LOCUS       DAWKQJ010000001        56452 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000001 DAWKQJ010000000
VERSION     DAWKQJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.001"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1005
                     /locus_tag="WJL20_000001"
     CDS             52..1005
                     /locus_tag="WJL20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ7682224.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1128..1451)
                     /locus_tag="WJL20_000002"
     CDS             complement(1128..1451)
                     /locus_tag="WJL20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682225.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVEIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1624..2985)
                     /locus_tag="WJL20_000003"
     CDS             complement(1624..2985)
                     /locus_tag="WJL20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ7682226.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAETIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3286..4986)
                     /gene="recJ"
                     /locus_tag="WJL20_000004"
     CDS             complement(3286..4986)
                     /gene="recJ"
                     /locus_tag="WJL20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ7682227.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLEAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5055..5768)
                     /locus_tag="WJL20_000005"
     CDS             complement(5055..5768)
                     /locus_tag="WJL20_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682228.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAG
                     AVLVLPMAALIAAANSSDK"
     gene            complement(5815..6384)
                     /locus_tag="WJL20_000006"
     CDS             complement(5815..6384)
                     /locus_tag="WJL20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ7682229.1"
                     /translation="MPSERGGILLKCAIGKNSRTKMFEQEDEWISKTQMKKQMNGLQD
                     LGMELTKLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIE
                     AFLAKLRGDDAAHNAFLQRVEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKK
                     EQEQNKPPKNFRALFQELKTVMENGDAEI"
     gene            6475..7806
                     /gene="pmbA"
                     /locus_tag="WJL20_000007"
     CDS             6475..7806
                     /gene="pmbA"
                     /locus_tag="WJL20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ7682230.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTLSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8103..8306)
                     /locus_tag="WJL20_000008"
     CDS             complement(8103..8306)
                     /locus_tag="WJL20_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ7682231.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8549..8851
                     /gene="clpS"
                     /locus_tag="WJL20_000009"
     CDS             8549..8851
                     /gene="clpS"
                     /locus_tag="WJL20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ7682232.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8853..11144
                     /gene="clpA"
                     /locus_tag="WJL20_000010"
     CDS             8853..11144
                     /gene="clpA"
                     /locus_tag="WJL20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ7682233.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAINKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQLEHRLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11183..14281)
                     /locus_tag="WJL20_000011"
     CDS             complement(11183..14281)
                     /locus_tag="WJL20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ7682234.1"
                     /translation="MSLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSDNSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQVKNWSKPESGGYIWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGNLH
                     PLKVTTIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EVRRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPIPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14278..15417)
                     /locus_tag="WJL20_000012"
     CDS             complement(14278..15417)
                     /locus_tag="WJL20_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7682235.1"
                     /translation="MGRSLTEIAEQLKGSNKKVQLIYAFNGTGKTRLSGEFKNLIAPT
                     SSEGADGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDNFSEIVFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKSSE
                     SELKFIITTHNVLFYNVLYNELKLKNKNGSYLLLKNEDGSFDILEKRGDSNKSFSYHL
                     HLKGVIEKAIQNQQVERFHFILLRNLYEKTANFLGYEEWSNLLPEDGRQNYFQRIINF
                     TSHSTLSNEEFIEPTRQEQETIKLLLKHLLDNYNFFQSNEQRDNP"
     gene            complement(15460..16678)
                     /locus_tag="WJL20_000013"
                     /pseudo
     CDS             complement(15460..16678)
                     /locus_tag="WJL20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(16844..17854)
                     /locus_tag="WJL20_000014"
     CDS             complement(16844..17854)
                     /locus_tag="WJL20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ7682236.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTIIAEQELEEEATVNFQLTVQNENGRKVSRKIAHYSLPMIIAVGYRVRSAQGTQF
                     RQWATERLDEYLTKGFAIDNERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(17851..19395)
                     /locus_tag="WJL20_000015"
     CDS             complement(17851..19395)
                     /locus_tag="WJL20_000015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ7682237.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPSQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSSRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTIKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(19469..20473)
                     /gene="rfaD"
                     /locus_tag="WJL20_000016"
     CDS             complement(19469..20473)
                     /gene="rfaD"
                     /locus_tag="WJL20_000016"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ7682238.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(20602..21633)
                     /locus_tag="WJL20_000017"
     CDS             complement(20602..21633)
                     /locus_tag="WJL20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7682239.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMSQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(21635..22870)
                     /gene="dcm"
                     /locus_tag="WJL20_000018"
     CDS             complement(21635..22870)
                     /gene="dcm"
                     /locus_tag="WJL20_000018"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7682240.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(22907..23878)
                     /gene="hldA"
                     /locus_tag="WJL20_000019"
     CDS             complement(22907..23878)
                     /gene="hldA"
                     /locus_tag="WJL20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ7682241.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(23933..24673)
                     /gene="pyrF"
                     /locus_tag="WJL20_000020"
     CDS             complement(23933..24673)
                     /gene="pyrF"
                     /locus_tag="WJL20_000020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ7682242.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(25199..25846)
                     /gene="adk"
                     /locus_tag="WJL20_000021"
     CDS             complement(25199..25846)
                     /gene="adk"
                     /locus_tag="WJL20_000021"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ7682243.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            26061..26900
                     /gene="htpX"
                     /locus_tag="WJL20_000022"
     CDS             26061..26900
                     /gene="htpX"
                     /locus_tag="WJL20_000022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ7682244.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(27203..27763)
                     /locus_tag="WJL20_000023"
     CDS             complement(27203..27763)
                     /locus_tag="WJL20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682245.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(27877..28269)
                     /locus_tag="WJL20_000024"
     CDS             complement(27877..28269)
                     /locus_tag="WJL20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7682246.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(28477..28869)
                     /locus_tag="WJL20_000025"
     CDS             complement(28477..28869)
                     /locus_tag="WJL20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7682247.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(28986..29396)
                     /locus_tag="WJL20_000026"
     CDS             complement(28986..29396)
                     /locus_tag="WJL20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7682248.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(29410..29847)
                     /locus_tag="WJL20_000027"
     CDS             complement(29410..29847)
                     /locus_tag="WJL20_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7682249.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(30258..31556)
                     /locus_tag="WJL20_000028"
     CDS             complement(30258..31556)
                     /locus_tag="WJL20_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ7682250.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(31660..32811)
                     /locus_tag="WJL20_000029"
     CDS             complement(31660..32811)
                     /locus_tag="WJL20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7682251.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            33146..34525
                     /gene="norM"
                     /locus_tag="WJL20_000030"
     CDS             33146..34525
                     /gene="norM"
                     /locus_tag="WJL20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ7682252.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            34704..35744
                     /gene="murB"
                     /locus_tag="WJL20_000031"
     CDS             34704..35744
                     /gene="murB"
                     /locus_tag="WJL20_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ7682253.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            35756..36406
                     /locus_tag="WJL20_000032"
     CDS             35756..36406
                     /locus_tag="WJL20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ7682254.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            36461..37258
                     /locus_tag="WJL20_000033"
     CDS             36461..37258
                     /locus_tag="WJL20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7682255.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPSGRRLKN
                     IIEPRHPTRREFFTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            37309..37875
                     /locus_tag="WJL20_000034"
     CDS             37309..37875
                     /locus_tag="WJL20_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ7682256.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            37895..38785
                     /locus_tag="WJL20_000035"
     CDS             37895..38785
                     /locus_tag="WJL20_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ7682257.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            38810..39568
                     /locus_tag="WJL20_000036"
     CDS             38810..39568
                     /locus_tag="WJL20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7682258.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWESDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(39623..40288)
                     /locus_tag="WJL20_000037"
     CDS             complement(39623..40288)
                     /locus_tag="WJL20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7682259.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYAEMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(40488..41384)
                     /locus_tag="WJL20_000038"
     CDS             complement(40488..41384)
                     /locus_tag="WJL20_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ7682260.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLHRLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            41453..42610
                     /locus_tag="WJL20_000039"
     CDS             41453..42610
                     /locus_tag="WJL20_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ7682261.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(42673..43674)
                     /gene="hemB"
                     /locus_tag="WJL20_000040"
     CDS             complement(42673..43674)
                     /gene="hemB"
                     /locus_tag="WJL20_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ7682262.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(43857..44141)
                     /gene="yhbY"
                     /locus_tag="WJL20_000041"
     CDS             complement(43857..44141)
                     /gene="yhbY"
                     /locus_tag="WJL20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ7682263.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            44251..44871
                     /locus_tag="WJL20_000042"
     CDS             44251..44871
                     /locus_tag="WJL20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ7682264.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            44935..46902
                     /gene="ftsH"
                     /locus_tag="WJL20_000043"
     CDS             44935..46902
                     /gene="ftsH"
                     /locus_tag="WJL20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ7682265.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            47124..47555
                     /locus_tag="WJL20_000044"
     CDS             47124..47555
                     /locus_tag="WJL20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ7682266.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSTTLVEAFVKEADRRYA"
     gene            47548..47826
                     /locus_tag="WJL20_000045"
     CDS             47548..47826
                     /locus_tag="WJL20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ7682267.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFSKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            47873..48451
                     /gene="pth"
                     /locus_tag="WJL20_000046"
     CDS             47873..48451
                     /gene="pth"
                     /locus_tag="WJL20_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ7682268.1"
                     /translation="MSNKIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            48511..48717
                     /locus_tag="WJL20_000047"
     CDS             48511..48717
                     /locus_tag="WJL20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ7682269.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKLCQTWLEEEQA"
     gene            48714..49076
                     /locus_tag="WJL20_000048"
     CDS             48714..49076
                     /locus_tag="WJL20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ7682270.1"
                     /translation="MNLPRNRFILLSALWFAGSIYSLLFKAAETAPPPFPHFDKVAHL
                     ALFFAQIWLLTKAFRTDNRPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFTARAACRPD"
     gene            49307..50722
                     /locus_tag="WJL20_000049"
     CDS             49307..50722
                     /locus_tag="WJL20_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ7682271.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            50841..51350
                     /locus_tag="WJL20_000050"
     CDS             50841..51350
                     /locus_tag="WJL20_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7682272.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            51517..52899
                     /locus_tag="WJL20_000051"
     CDS             51517..52899
                     /locus_tag="WJL20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ7682273.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(52919..53674)
                     /locus_tag="WJL20_000052"
     CDS             complement(52919..53674)
                     /locus_tag="WJL20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7682274.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(53684..54400)
                     /locus_tag="WJL20_000053"
     CDS             complement(53684..54400)
                     /locus_tag="WJL20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7682275.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(54390..55217)
                     /locus_tag="WJL20_000054"
     CDS             complement(54390..55217)
                     /locus_tag="WJL20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7682276.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSSLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            55502..56410
                     /locus_tag="WJL20_000055"
     CDS             55502..56410
                     /locus_tag="WJL20_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ7682277.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13537 a        15341 c        13763 g        13811 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tatccgtatc cgtttcttta tccgtccaac cgatacatca atcgaatttt
     1141 ctgcatcgga ttgcctcatc ttcgcaaaac attgccgaag tttcgcggtt gcacgcctga
     1201 cgcactttct gccgcgccac gccttcattc cgatcgcttg cctcaaacgt cttctgaaaa
     1261 ggcgtaacgc tgcatacata catggtggaa tttgaaccga aaaggtcgat ttcgacggta
     1321 ttgccgccgc tttccaatac ggcgacacgg tgttccaact gcgccagacg tgcttccaaa
     1381 tcgccgattc tgtcggcaaa tgcaggcatg gcggcacacg cggtcaaaat gcccaaaata
     1441 cattttttca tcaatttttc cttgttgctt gaaccctgcc cacccggaca tcctttttcc
     1501 gagaggcagg gttagacttt atttacgggg atgaatcccc ttacaaatca ggataatgca
     1561 tagaacggca ctttatgctt catcccatgt gttgaaactt cattgctcct tgtctgttga
     1621 cccttattcc caatccgttc cctcaacctt cggcttgggt ttgcgcctgc gacggcggcg
     1681 ttttttcgcc tgtccttcgt ttttttcgtg tcgggcggcg gcttcgtttt tggtcatctc
     1741 cgaccgctgt tccgtcgatg ccgtctgaaa cgccgtccac cactcggcaa gggcgcggtc
     1801 cgcattgccg gtttcggcgc gcaagagcag gaaatcatag gcggcacgga aacgcgcctg
     1861 tgcaaacagt ttgtgcggac gtgcgccttt gcgattttca aactgcggct ggaacatcca
     1921 aatttcgcgc atcgtggcgg aaaagcgttg cggcacgccc caaccgcgtt cgacggtttc
     1981 gcgcatcgta ttgattgcat cggacagggc gggcgcgggt ttcaaaccct gttgcagatt
     2041 gcttttccaa tggcgttcca actcaggcca catcagagcc gccaatacaa aaccgaccga
     2101 aaccgatttg tcggcacgca gccgctcatc ggtatttttc agggcaagca ccgtcatttt
     2161 tccggcgatg ccgtctgaaa cacgcaaggc attgagcagc ggatggatgt cgtccggtat
     2221 gtcaaatccg ttcaaacgtt tcagacactc gcgcgcgtgc cctgaaaaca gcagtttcat
     2281 gatttcgtca aacagcctcg ctaccggttc gtgcttcaga cggcatatcg attcggcaat
     2341 cggtgcggtg gtttcttccg acagctcaaa gcccaatttg cccgacaggc ggatggcgcg
     2401 caaaatcctg acagggtctt cctgatagcg ttcggcggca tcgccaatca taaccagcct
     2461 gcgggcggca acatcggcaa tcccgttgtg gaaatccaaa atctcttctt tttcaggatc
     2521 gtaatacaag gcattgcagg taaaatcgcg ccgcatcgca tcttcttcga tgctgccgta
     2581 ggtgttatcc ttcatgattc tgccgcgcgc gttctgatga accttcgcac cgccccggaa
     2641 agtcgttacc tctatggttt ccgcgccgtt cataacgtgg acgatttgaa aacgcctgcc
     2701 gatgatgcgg ctgcggcgga agagtttgtg cacctgttcg ggcgtggcat cggttgcgac
     2761 atcgaaatcc ttgggttcga tgccgagcag caggtccctg accgcgccgc cgaccacata
     2821 agcctgaaac cccgcccctt tcaggcggcg tatgacgttt tcggcggcaa agctcaacat
     2881 ttcggcacgg atgttgtgtc tttcggcagg aatgaccgtt ttactttccg cttttttact
     2941 gctccgaccg gaaggcagca tcttattcaa ccattttttc agcataaata ttctaacccg
     3001 aagagcggca tctccacggg caaccgtgtt cagacggcat caggcggtac gattccgcaa
     3061 gatgccgtaa ccataaacaa aaggcgtaga aactacgccg tccgaatcac gccgtcctcg
     3121 cggcggcaac gcgtcattat aacagattgc cctccaaaaa aatcagaccc tgcttttgtg
     3181 gcagagtctg aaatttgatg cgccttctcc gattgggaac attcccgtcg tacaaacagg
     3241 caaatgaagg aacagaaatc acattcaaac ggcgttccgc cgcctctacg ccgcttccca
     3301 gtagtcgata taaagctgca attcgaggtt gttgcgccat tcgttggcaa cggggcggta
     3361 caccgtgcgg atgtattcgg gaatgtcttc gctgcaccgc cagaacattg cttcaaattc
     3421 gcagccgtct ttttgcagcc acgctttttt gtgtttgcct tccgcgccca aaggctgctg
     3481 gcggacgacg tggaactcgt cggtaaagct cggcggcgcg aagccctgcc cccaaacgtg
     3541 acgggcaagg ttttgcgcct gttccaacgt gatgtcgcag gcgggcaggc tgccgtcggt
     3601 gatgaaggtt tgcgacaaat cgtcttcgca caccatttcg cgcacggctt cttcaaaggt
     3661 cgtctgaaac gcgggaatgt tgtgttcaag tatgctcaaa cccgccgcca tcgcgtgtcc
     3721 gccgaatttc aaaatcaaat cgggatggcg tttggacacc aagtccaaag catcgcgcag
     3781 gtgcaaattg ggaatggaac gtcccgaacc gcgtacttcg ccgttgtcgg caggcgcaaa
     3841 cacgatggtc ggacgataaa aacggtcttt gaggcggctg gcaacgatgc cgaccacgcc
     3901 ttgatggaag tcgtcgcgat acgccaccaa agtcatctga cctgaaggca gggtttcagg
     3961 aaaagcattc agcgcgtctt gcagcataga ctgctcgatt tcgcgacgct cgatattgag
     4021 gttgtttaac tgagccgcca gttcctgagc ttcggaatca tctcgcgcca acaggcaggc
     4081 gatgccgacc gacatatcgt ctagccgtcc ggcggcattg atgcgcggcc ccaacgcaaa
     4141 gcccatatca aacggctgcg ctttgcgcca gtcgcgccgc gccacttcaa acaaggcgcg
     4201 gataccgggg cgcattttgc ccgaacgcat ccgtttcaaa ccttgcgaca cgaggatacg
     4261 gttgttgtga tcgagggaga cgacgtcggc aacggtaccg agtgcgacca aatccaaaag
     4321 gtcgcccaga ttcggctctt ttaggccgtc tgaaaaataa ttgcggcggc gcagttcggc
     4381 acgcaaagcc atcagtacat aaaaaatcac gcccacgccc gccaagcttt tgctttggaa
     4441 accgcagcct ttttggttcg gattgacgat gatgcagtct ggcacggtct cggcaggcag
     4501 atggtggtcg gttacaatca catccaaacc caattcctgc gccctcgcca cgcctgcgat
     4561 gctggcgata ccgttatcaa ccgtaatcag caaatccaca ccttgctctg ccgcgatttc
     4621 agccagctcg ggcgttaagc cgtagccgtg ttcaaagcgg ttgggcacga ggaaatccac
     4681 tttcgcgccc atcgcctcca aaccgtccaa accgacggca cacgccgtcg cgccgtcggc
     4741 atcatagtcg gcgacaatca ggattttttc ctgacgctca accgcatccg ccaaacggcg
     4801 ggcggcggct tcgcaattcg tcagcgattg ataaggcagg agggaagcga gtttgtcgtc
     4861 caattcggca ggactttgca caccgcgcga agcacaaagc cgggcgatta aaggatcggc
     4921 accggcggtg agcaaatgat taaaaacgtc ggtattgacg gagcgggttt ggattttgac
     4981 ggacataacg tggaacttta cagtgcgggt gtgcttgaaa tgccgtctga aggttttgca
     5041 gacggcattg ccgttcattt gtctgaggaa ttcgcggctg caatcaacgc cgccataggc
     5101 aagaccagta ccgcacccgc cgcatccagt atcaacgtgg tgggcgtata tgcaatattt
     5161 tcaaacaact tggatttgtc ggtatgtttt ttcgtaaccg tgtaataaat atcggcaggc
     5221 acactttgct caaaatgata atcggcgttc agtttttgcg gtgtggcgta gtatttgcct
     5281 ttggcggaga cgcagcgcgt gtaaatggtc cgattgtcga gttcgaccgc ttcaaactca
     5341 agctgtttca gcttggcgat gtcggcaggt ctgtcggtat cgtagcgcag gcaaaggtcg
     5401 tcggtactga aattctggct ggcgggcgat tcgagtttga ccggcagggc ttggtaggca
     5461 aagcgcgggt tgggctcaac catttgaaac tgcttgtcca acccggcctt caaaatgccc
     5521 gtcagcttcg ccgaatcttc aggattgacg acgaaccagt atttcccgcc catcatcacc
     5581 aggctgccct tttccaattg ggcattgtct ttggcaacca caccgaaggc gcggatttgg
     5641 tctttgtcaa cgtgtttata ggcggtcgtt tggctgaacg ggtcgttcat gccccacatc
     5701 attgccgtac agccattcag catcaaggtt gccgccaaaa cggcggcggt cgttttacgg
     5761 aacaacattt tatcctccaa tctaaataac ggcggatgtc gtctgaaaat atgcctaaat
     5821 ttccgcgtcc ccgttttcca taacggtttt caattcttga aacagggcgc ggaaattttt
     5881 cggcggtttg ttttgctcct gctctttttt ggtgttgcgg atcaaggtac gcaatttacc
     5941 ggcgtccgca tgcggaaaat ccgacataaa ctgcgtcaac gcgccgtcgt ctgccaacag
     6001 ccgtacgcgc gcctgttcca cgcgttgcaa aaaggcgttg tgcgccgcat cgtcgccgcg
     6061 cagcttggca agaaacgcct cgatgggcgc gggatccgtg tcgcgcatca gcctgccgat
     6121 aaattgcgcc tgccgcttga gcgcgccgtt ggacgtaatt tttttatagg taacgacggc
     6181 ttcgtacaaa tcttcgtcca agccgatttt tttcagcgta tcgtttgaga gcttggtcag
     6241 ttccataccc aaatcctgca aaccgttcat ctgctttttc atttgggttt tactgatcca
     6301 ctcgtcttct tgttcaaaca tcttggttct cgaatttttt ccaatagcgc attttaacag
     6361 aatgccgccg cgttcagacg gcattttgca ccgcacgccc tgcaagcggc gcgcttttgc
     6421 ccctgccgcc cgaaacggtt aaaatgccgc aaagcatcat caagaaagat ttctatgctg
     6481 ttcaaccaca ccgcttccga actcctcgac ctctgccgcc gcacgctcga cttggcaaaa
     6541 gcgacgggcg caaccgccgc cgaagccgat ttcagcgaat cattgggaca aagcgtcagc
     6601 gtgcggctgg gcgaaatcga acaaatcgag tttcagcagg acaagtcgct ggacattacc
     6661 gtttacgtcg gcaaacgcaa aggccgcgcc ggcaccgccg atttctccga acaagccctg
     6721 cgcgacaccg tccgcgccgc cgccgacatc gcccgccaca ccgccgaaga cggttgcgcc
     6781 ggacttgccg atgcctgtct gatggcaaaa cacatcggcg accccgacct ttaccacgaa
     6841 tgggatttgg atacggaagc cgccgtcgag ttggcaaaac aatgcgaaca agccgccctg
     6901 aatgaggatg agcgcatcga aaactccgaa ggcgcggcgg tgcaaaccgg ccattaccaa
     6961 tacgtttacg gcaacaccca cggttttgcc gcacaccggc aaggcacgca ccacagcatt
     7021 tcctgcagcg tcgttgccgc cgacgaaaac gggatgcagc gcgactactg gtacgattcc
     7081 gcctgccgcc atccggatat ggacagcccc gaaaccatcg gtcaaaccgc cgcccgccgc
     7141 actttgcgcc gactcggcag ccgcagcatc ccgacaggca gctaccccgt cctcttcgat
     7201 accacccttt cgggcggtct tatcggacac ctcgtcggcg cactctccgg cggcgcgctc
     7261 taccgccaaa gcagtttcct gatcgacagc atcggcaaaa aagtcctgcc cgatttcctc
     7321 aacctgcgcg aagaaccgca catcccccgc tctttccgca gcagctattt tgatgcggaa
     7381 ggcgttgcca ccgcaccgcg cttcgtgatt caaaacggca ttgtcaaagg ctatttcctc
     7441 agcagttaca gcgcgcgcaa actcggtatg cagaccacgg gcaacgccgg cggcgcgcac
     7501 aacctgtatt tgaaccatac gcacgaaacg caatccgacc tgctgaaaga aatggggacg
     7561 ggattgttgg taaccgaact gatgggacag ggcgcgaaca ccattaccgg tgactactcg
     7621 cgcggcgcgg cgggtttttg ggtggaaaac ggcgtgattg cctaccccgt ccacgagatt
     7681 accgtagccg gacgcttgca ggatatgtac cgcgacattg tcggcgtggc ggatgacgct
     7741 ttgcggcgtt cgtccaacaa aatcggctcg attctgattg cggggatgac ggttgccgga
     7801 agctgaacgc cctgccgatg ccgtctgaag acgaaggcag cacgttttgc cccgtgcctg
     7861 cccgtttgcc gttttatgcc gcaccgcctt ttgccgacaa cattgcacat tttttcatca
     7921 gcactgattt tcccttatcc gtcaaaatac taagccactt gtccacaaca gggtacacac
     7981 aagttcagga taaggcgtgc ggggattggg gtttttccgc cgccggcaaa acggcagaca
     8041 aaaacggcgg gacattgcct gtcccgccgt tttcaaatat acatttgacc gtacatcagg
     8101 aattaagcag cctgaatgtt ggcggcctgt ttgcctttag ggccggtggt tacgtcgaaa
     8161 gagacgcgtt ggccttcttt cagggttttg aaaccttcca tattgattgc tgagaagtga
     8221 gcgaacaaat cttcgccgcc ttcatcaggc gtgatgaaac caaaaccttt agcgtcgtta
     8281 aaccatttta cgataccggt tgccattaga aacttcctat actcaaaaat taacaaaatc
     8341 agcaaaacaa ggcatacggc aaaccgagcc gtctggcgtt ccttccagct tgatatactc
     8401 ggcggatgcc caatcctgct taaccgtctt tttacatttg ttaagacaaa acgtcaagct
     8461 atgttttcaa aaaagtggga aaataggcaa aagtaccgaa aatacaacaa aaatgccgtc
     8521 tgaacgcggc tttcaaatgg gacacactat gaccgcgcaa caccaatccg acacgctctt
     8581 gcaccgtctg aacaccctgc cgccgaaacg ttacggcgtt ttcctattga acgacgatta
     8641 caccacgatg gaatttgtcg ttgaaatcct gaccgaaatc ttcatgctcg gacaagaaca
     8701 ggcggtagcg gtaatgctct tggttcatca cgaaggcaaa ggcctgtgcg gcacttacac
     8761 gcgcgatatt gcccaaacca aacagcaaca agtcatgcag cgggcaaaag ccgaagggca
     8821 tccgctgcaa tgtattgtcg aggagattta atatgcttgc acccgaattg gaacagattt
     8881 tgcagcagct ttaccgcgag gcgcgtaagg ctcattatga atttatcagc ctcgagcatc
     8941 tgcttttggt actcatcgaa gaagatgcct ccgtgccgaa cgtcttaaaa ctctgcggcg
     9001 cggatttgaa agtggtgtcc gaacagctcg ccgccagcgt tgccgaaaac acccccctga
     9061 ttcccgaaca ccttttagac acggtcgaaa cccggcccac gctcggcttc caacgcgtga
     9121 tgcaacgggc gatggtgcat acccagtctg ccggaaaagc cgcagtcgaa ccgttggacg
     9181 ttttggtcgc gctgatgagc gaaaccgaca gccacaccgt ctatttcctc aagctgcaat
     9241 cggttacgcg ttttgaagtt ttgcgctgta ttgcccacgg ctctcccgat gaagatgaag
     9301 acgatggcaa ctattcttca gacggcatgg acgacgataa tgaaaaccgc accaaaccgg
     9361 gcaaaaaccc tttatcggcg tacaccgtca acctcaacgc cgaagtcaaa gccggccgta
     9421 tcgacccttt gattggtcgc aaacacgaaa tggaacggct ggtgcaaatc ctatgccgcc
     9481 gccgcaaaaa caatccgctt ttggtcggcg aagcgggcgt gggcaaaacc gcgctggcgg
     9541 aaggtttggc acatcaaatc gtcaacggcg gcattccaga cgcgctcaaa gatgccgaag
     9601 tgtacgcgct ggatatgggc tcgctgttgg cgggcacgaa ataccgcggc gactttgaag
     9661 cgcgggtcaa atccgtcttg aaacagctcg aaaaaatccc gcacgccatt ttgtttatcg
     9721 acgaaatcca caccatcatc ggcgcaggca gcaccagcgg cggcaccatg gacgcgtcca
     9781 acctgctcaa acccgcgctg gcaaaaggtt cgctgcgctg catcggcgcg accacttacg
     9841 acgaataccg caccattttc gacaaagacc acgccttaag ccgccgcttc caaaaaatcg
     9901 acgtggtcga acccaccgtt tccgaaaccg tacaaatcct gcgcggcttg aagccgatgt
     9961 ttgaagcctt ccaccaagtc cgctatacgc aaggcgcact cgaagccgcc gccgaactct
    10021 ccgcacgcta catcaacgag cgtttcctgc ccgacaaagc catcgacgtg atggacgaag
    10081 caggcgcggc gcaacggatt ctgcccaaat ccaaacagaa aaaagtcatc ggcaaagcac
    10141 aaattgaaac cgtcatcgcc aaagttgcgc ggattcccga aaaaaccgtg tcgcacgacg
    10201 acaaacaagt gctgcaattc ctcggtcgcg atttgaaaaa catggtttac ggtcaagaaa
    10261 acgccatcga cgcgctggtt gccgccgtca aaatgtcgcg ttccggcctt gccctgcccg
    10321 acaaaccgat aggcagcttc ctcttctccg gcccgaccgg cgtgggcaaa accgaagccg
    10381 ccaaacagct tgcctactcg ctgggcgtac cgctgcaacg ctttgatatg tccgaataca
    10441 tggaacgcca tgccgtatcg cgcctcatcg gcgcgccacc gggctacgtc ggctttgaac
    10501 aaggcggcct tttgaccgaa gccatcaaca aacagccgca ttgcgtattg ctcttggacg
    10561 aaatcgaaaa agcccacccc gacattttca acgtcctcct gcaagtcatg gacgcaggca
    10621 aactgaccga caacaacggc aagagtgccg atttccgcaa cgtcatccta attatgacca
    10681 ctaacgcagg tgcggagagt ctcagccgac ccagcctcgg ctttaccgcc aaacgcgagc
    10741 gcggcgacga aatgcaggct atcaacaagc tcttcacgcc cgagttccgc aaccgcttgg
    10801 atgcgattat cccgtttgcg cccttatccg aacccgtcat caccaaagtc gtggacaaat
    10861 tcctgctcca gctcgaacac cggctcctcg acaaaaaagt cgaagccgaa ttcacgtcgg
    10921 cattgcacaa atatctggcg gaaaaaggtt ttgacccgca aatgggcgcg cgcccgatgc
    10981 accgcctgat tcaggaaaaa atccgcaaac cgctcgccga cgaactcctg ttcggcaaac
    11041 tatccgacgg cggcttcgta cggatagact gggatgcggc aaaagaagaa gccgtgttga
    11101 agtttaagaa aagcaaggtc aaaataaaaa ccgcctccgc ataaaatcaa aaatgccgtc
    11161 tgaaatttca gacggcattt tgtcaaatat cggtccctat tccggcaaac ttctccacaa
    11221 acgccgcaat cttttgaaaa acactttgtt tcttcgtcag atattgcggg ttgagcggac
    11281 tcattttcgg cagggtttcg gtcagttccg tgccgttttc gctggcatag ccgcgtttga
    11341 gcgagctgat taaatagcgt ttggcggcgg tttcgttcag gccttcggcg gcaatcaatc
    11401 ctgccgcttc gtgccgcatc acctcttgcg cgtaggtgta gaaggtttcc agtatggcgg
    11461 gaacgtcggg tactttgtcc aaatccgtat cgttgatgaa atccacaatc agaccctctt
    11521 tggcgcggtg gccgatgctg gcgcggatga tgcggcggat ttcttccacc agctccgctt
    11581 tgcctttgat ttttttgtgg tgttcgaaaa ccagttgcag gatgtaatcc agattgattt
    11641 cctgtgattt gagcaaatcc acctcaaaaa ccacatcgtc ccaatcgatt tttgactgtt
    11701 ctttctgttc gcctgctttt tggcggcgca accaatcgcg gatgtcgttg taggcggaac
    11761 ggtagtcctg caccgccctt tcagacggta tcggcacttg ccgcatttcc tgcacgtctt
    11821 catcgctcaa gtagtatttt tcttgaaacg ccttcatcgc atcttcgtcc gccgcgtcca
    11881 cactctgcaa ttcacgcaac gcggcaaatt catcgtagtt ctgcaatacg ttttccgccc
    11941 gcaggtattc gccgaagagt ttggcaaaat cttttttgtc tttttccgtt tcgattttgt
    12001 cgggatcggg gaaacgctcg cgcaattctt ttgccacatc caaataaccg cgccgcactt
    12061 cgcccgtccg atcatcgata tagccgttca tgtattcttc gtagcttttt tccaacacca
    12121 cgtttttggt gtttttatcg ccgaacaggg taatcgcatc aatggttgcc tgctccaaat
    12181 cgcggaagca gacaatattg ccgaaggttt tggtggcatc gtaaatgcgg ttggtgcgcg
    12241 aaaacgcctg catcaggccg tgatagcgca ggtttttatc gacaaacagc gtgttcagcg
    12301 tcggcgcgtc aaaacccgtc aaaaacatac cgaccacaat cagcaaatcc acttcctggt
    12361 ttttcacccg ttttgccaaa tcgcggtagt agttctgaaa ggctttgctg tccgtgccga
    12421 aattggtttt gaaacaggcg ttgtaatcgt tgatggcagc ctgcaaaaat tctttcgcgc
    12481 tgctgtccat cgcttccggt tcaaaggtct catcaacaat ttcaccgacg gcgttctgct
    12541 cttcgttggc cgcaaaggaa aaaatggtgg tcactttcag cgggtgcagg ttgcctgcct
    12601 gttgtgtttt gaacgcttcg taatagcact tcgccgcatc cacgctgctg accgcaaaca
    12661 tggcgttgaa gcctttaccg cccgcattca ggcggtgcgt tttctgcctg aactggttca
    12721 ggatatattg cgtgatttcg cggatgcgtt cggggtgcag cagggcttgg cggttttcgg
    12781 cagcactcag tttcttctca tcctgttccg cttccacggc tttgaactgc gggcgcacat
    12841 cgttgtaatc cactttgaat ttcaatactt tttcatcgcg gatggcatcg gtaatcacat
    12901 aagaatgcaa ctcacgcccg aacacgcccg ccgtggtttc cgcgcccaaa gcgttttcgg
    12961 gaaaaatcgg cgtaccggta aagccgaact ggcagaattt tttaaatttc tttttcagat
    13021 ttttttgcgc ttcgccgaat tgcgagcggt ggcattcgtc gaaaataaag acaacctgct
    13081 ggtgataaac cggcagatta tcttcactct tcatcaggtt gttcagcttt tggatggtgg
    13141 taacgatgat tttgttgtcg tctttttcca aattgcgttt caagcccgcc gtgctttccg
    13201 aaccgttcac gctgtcggga gaaaaacgct ggtattcctt catcgtctga tagtccaaat
    13261 ccttcctgtc caccacaaag aaaaccttgt cgataaacgc cgattctgtc gccagacgcg
    13321 ccgccttaaa gctggtcagc gttttgccgc tgcccgtggt gtgccagata tagccgccgc
    13381 tctccggctt gctccaattc tttacctgcg ccgagctgtt gattttccac aaaatgcgtt
    13441 cggcggcggc aatctgatac ggccgcataa tcagcagcgt atcattcgca tcgaacacgc
    13501 tgtaatgcag caaaacgctc agcaatacgc ttttttgtag gaacgtggcg gtaaagtctt
    13561 tcaaatcctt aatcggatga ttgtccgacc gcgcccaatt catcgtgaaa tcgaagctgt
    13621 ttttgtcgcg cttggtggtg ttggcgaaat agcgcgtgtc cgtgccgttg gaaatgacga
    13681 agatttgcag gaatttgaac aacgaattgt cgctgttgaa gctctctttg ctgtaacggt
    13741 gcacctgatt gaatgcctcg cgcaccgcca caccgcgctt tttcaattca atctgcacca
    13801 gcggcaggcc gtttaccaac acggtaacgt catagcggtt tgcatgcgtg cccgtctgct
    13861 caaactggtt gataagctgc acatggttgc gggcaaggtt tttcttgtcc agcagataaa
    13921 tgtttttcag acggccgtca tcgaaagcga aatcgtaaat atggtcgtcg tggattttgc
    13981 gggttttatc ggtaatgttt tcagacggcc tgtccagata ttccgtcaaa aaacgcgccc
    14041 attcgccgtc tgaaaacgcc acatcgttca gccgctgcaa ctgcgcgcgc aggttttcca
    14101 gcagcctgct ttggctgttc aaatccttgc ggtattcgta accctgattc tgcaaatcgg
    14161 cgattaactc cgcttccaac cggttttccg attggtagct gcccgactgt tcgatttttt
    14221 catattggtc gagcacgatg aaattcggcg tttcggcgat gggtttggtt tcgaggctca
    14281 tggattatct ctttgttcat tactttgaaa aaaattatag ttatccagca agtgtttcag
    14341 aagtaatttg atagtttctt gttcttgccg tgttggttca ataaattcct catttgataa
    14401 tgtagaatga cttgtgaagt taataatacg ttgaaaatag ttttgtctac catcttcagg
    14461 cagaagatta gaccattctt cataacctaa aaaattagct gttttctcat acaggtttct
    14521 tagtaaaata aaatgaaatc tctctacttg ttgattttgt atagcttttt caataactcc
    14581 ttttaagtga aggtgatatg aaaaactttt attggaatcc ccacgttttt caagaatatc
    14641 aaaactacca tcttcatttt ttaacaaaag ataacttcca tttttatttt tcaactttag
    14701 ttcattgtat agaacgttgt aaaataaaac attatgtgtg gtaataataa actttaactc
    14761 tgattcgcta ctttttatca aatcagccaa atcaaccgct tgctgaatca gatgattttc
    14821 atccaaagag ctgacagggt catcaataaa aatatattcc aaatcgtcaa actgatctgt
    14881 ggaacgtcct tcttcgctat cttctggaat attcaattca gcgatgactt gtctgattaa
    14941 tacatagaaa atgctccaaa taaaattact ttcttcacct ttggaaattt tgatattttc
    15001 aatctgctca tcattaccac gagcaaaata aaatacaatt tctgaaaaat tatcattaaa
    15061 atcaggagtc aacttatcat ctgtataata ttgaaagttt ttaataacag ctccatccag
    15121 tccattatcc ctaagcaacc agtcggtaaa actattcttt tgaattttta atcttggagc
    15181 ttcgttcgct aacaaatcat tgtcccaaaa gaataaatcc tcagtaaatg cattatagta
    15241 gagaaatttt cttcttgttg gctctccgtc tgccccttct gaactggttg gagcaattaa
    15301 attcttaaac tctccggata aacgcgtttt ccctgttccg ttaaaggcat agataagctg
    15361 aaccttttta ttgcttcctt tcaattgttc agcaatctca gttaaacttc ttcccattat
    15421 atttccttat caatattttc agatgacatt atcccaattt caggctgcct tggggaaggc
    15481 aagcagctgt tcacggtaat attcgtattg ttttcggcgc agggtgattt cgtacggcag
    15541 gccttcgctg atggagtggg tcagggtgtc gaatttgtcg aggatggcga cgattttttc
    15601 ctgttcgggg agtgagggga tggggatttt aaattcttta acctttgcat ctgatattgc
    15661 tggataagct gagccacttt gatttacttc aacatattga ttaaaccttt ctgttgagat
    15721 ttgataataa atccactttg gcaatacctc tttgcaattt gctcttaata cacaatatcc
    15781 cgtgcttgca atttcaccag aaaatttctt actaattatt gtaaaacgtt gtagcgttgg
    15841 ccgagtagtt gcaaaaatca catcatgttc ttttacaatt ttttgagctc tacttggcgc
    15901 attatttgct gatattgcaa tagtatcagt aatggtttta tattcccgac taactgaagt
    15961 caaatcaata tattggtaaa ttttatctgt atatttccat ttaatatttt cagtaggtaa
    16021 ggttacctcc cccaacgtct tccaaaccac atctttcaga cggcattgat agccatcagc
    16081 tccccccccc gatttgattg tcaaaatcta aaagaaagtc acggtaatac tggtattggc
    16141 gtttgcgcag ggctaattct gcttccagcg tagcttccag ctcagtgaat ttgtcaagta
    16201 tttttacaat tttttgttgg gtttccaggg gtgggatggg gatttcctta ttggaaaaat
    16261 tactaatcca ttgtcgttta tggtctccat cggttaatga attgggaagc gtattcaacc
    16321 agtaataaac atatttcagt aaaacgtcct gttcattttt tgaggtaatt attttcatag
    16381 cagaagattt tactttgaaa tcaaaatcaa cccatttatt tgctgttgtg aaatcatcaa
    16441 agataatgac aggagacccc gaggctttat atatgccttc tgtttcatct gtataaccca
    16501 aaataaatgt tttgcctgca gttaaaacag gtgtatcaaa cttatcatca taattcttag
    16561 aagcaactaa atattttgtt ggctgttcgt aatcagccac ctcccccaac ggcttccact
    16621 ccaccggtgc cgtctgaatc atctcaatca attttttcgc tttgctttgc atatccatgg
    16681 ttgttttccg tttctttccc gctgtggcgg gatgtggggc ggttgtgggg tgcttcggct
    16741 cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc gtcctgcttt caggctgcct
    16801 gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg tagttatttt gtgcggtctt
    16861 ttttctgttt gatctgttgc acggctgttt tttccaacgc tttaacgctt tccagatagg
    16921 cttgctccac cggcgacagg atgcgcgctt cataggcgcg gtattcgcgt tcggctttct
    16981 gctctgcctg tttgcggctg atgctgcctg caccctgtaa tgtgccttgt ccgtacagcc
    17041 cggaaaattt gtccaattcg gctatccagt cgcgcatata catggggctt tgctcctgcg
    17101 ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg gttcaggcgg aacagttcat
    17161 cttcggtcag gtagtttttg gcgattttgg cttcattcag ggtagggatt gcaccttgaa
    17221 aggtggtcag ccccataaag tctttgctgc tgtcggcacg gctgtatatc agttcggctg
    17281 cggtttgccg actggcggca tagtgcagtt tgttttgaac ggcggcaaaa aaggtttggc
    17341 tttcgctgct tttggggttg tagtcttggc tggtggcata taaatcaagc acttgccggt
    17401 atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt gagcagttct ttccaatagt
    17461 cgccgccgcc tgtgcctttc aggcgttcgt tgtctatggc aaaacctttg gtcaggtatt
    17521 cgtccagccg ttcggttgcc cattggcgga attgggtgcc ctgcgcggaa cggacgcggt
    17581 agccgacggc aataatcatg ggcagggaat aatgggcgat tttgcggctt accttgcgcc
    17641 cgttttcatt ttgaactgtc aactggaagt tgacagttgc ctcctcttcc aattcttgct
    17701 ctgcaataat ggttttaatg tgtttgctga tattttgttt ggtggtttgg tatagttctg
    17761 ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc ctgcaaggca aattgggcag
    17821 tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca atctctgcaa tcacttcgtc
    17881 aatttcgcgc cgcagccgtt cgattttggc gacggtttcg ccgatttcgg cgttaagctg
    17941 tttgatgtcg ataatttcgc gtgtgtcttc gggttcaacg tagctgctga cggcgaggtt
    18001 gtagccgttg tctttgatgg tttgctgggc ggcgttttgg gcgatatgcg gcacatcggc
    18061 tttgtcggcg aagagtttga cgatttcggc aatgtgttct tcggttaaga cgttgttgtt
    18121 ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg atgtcggtat tgtctttgtg
    18181 tttggacaaa accaggatat tgacggcgat gccggtgccg taaaagagat tgggcgcaag
    18241 ggcgatgacg gtttccacgt agttgccctc caccagatat tggcggattt tctgctctgc
    18301 gccgccgcga tagaaaatgc cggggaatga gacgatggcg gcgcgacctc tgctagaaag
    18361 gtagtttagt gcgtgcagga tgaaggcaaa atcggctttg gatttcgggg cgagtacgcc
    18421 tgctggggca aagcggtcgt cgttgatcaa ggtggggtcg tcgctgccta tccagttgat
    18481 ggaataaggc ggattggaaa cgatggcatc aaagggtttg ctgtctttga gtttgggatt
    18541 ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa ttgacgttgt gcagaaacat
    18601 attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc tgcccgaaga agccttcttc
    18661 gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc aggctgcccg agccgcaagc
    18721 tgggtcgtag attttgttta ctttctcctg cccgtgcacc gccagccgcg caatcagctt
    18781 ggatacgctt tgcggggtga aaaattcgcc gccggatttg cctgcgttgg cagcgtagtt
    18841 ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg cggtggtctt caaaactgcc
    18901 gaaatcgagt tccgccacgc cttttaggac ggcggcaagg cgtttgttct tgtcggcaac
    18961 tgtgctgccg agccggctgc tggtggtatc gaagtcgtca aacaggcctt taatgtcttg
    19021 ttcggacgga tagccggagg cggagctttc aatcgcggta aaaatttctt tcagcttggt
    19081 gttgagctct tcgttttgat gggcttcggc ggcaatattg caaaaaagct ggctggggta
    19141 gatgaaataa cctttgactt tgacggcatc gtctttgatt tcgggtgtga tgatgctgtc
    19201 cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc atatagtcgg tgaagttttc
    19261 gctgataaat cggtagaaaa gtgtgccgag aacgtattgt ttaaagtccc agccatccac
    19321 cgcgccgcgt acttcgtcgg caattttcca aatttggcgg tgcagttggg cgcgttgttg
    19381 catttcggtc atcatcgaaa tccatataaa agttaaacaa atcaaaatcg cctgatattt
    19441 tcagacgatt tttttacggg cattcaaatt aagccaaatt ttccagcatc catttgacgt
    19501 agcggtcgac gcctgatttg acatcgaaaa attcttcctt atatccggct tcgcgcaatt
    19561 tggtgatgtc ggcttgggtg aagctctggt atttgccttt gagtgcgtcg gggaagggga
    19621 tgtagcggat aagttcttct tccaccagct ctttcaagct catttcaggc ttgccttcgg
    19681 cggcgcggca ggcgttgacg gtggcggcgg cgagttcgtt aaactgttgg ctgcggccgg
    19741 taccgaggtt gtagatgccg gaaagttcgg gatggtcgaa gaagtagagg ttgactttgg
    19801 caacgtcttc gacgctgaca aagtcgcggg tttgttcgcc gttgccgtaa ccgtcattgc
    19861 tgccgaacag gttgacgtaa ccgtgttcgc ggtattggtg gaaatggtgg aaggtgacgg
    19921 atgccatgcg gcctttgtgt tgttcgtgtt gtccgtaaac gttgaagtag cggaagccga
    19981 cgacttgggc ggtgagacct tctttcatgc ggcggcgcaa tacttggtca aacaggaatt
    20041 tggagtagcc gtagacgttg agcggttttt ccagttcgcg ctcttcgcgg aagatttcgc
    20101 ctttgccgta aaccgccgca ctggaggcat aaaggaaggg aatgcgttcg tcctgacacc
    20161 agtccaacaa atccagcgtg tactggtagt tgttttccat catatacaaa ccgtcgtgat
    20221 tcatggtgtc ggaacacgcg ccttgatgga aaacggcttc gatgttttga taaggtaaaa
    20281 tgtgttccct cacttggcgg atgaattcat gtttgtcgag ataatgggcg atttcgcact
    20341 cggcaaggtt tttgaatttt tcgcctttgc tcaaattatc gacggcaaca atgtcagtaa
    20401 taccgcgttg attaagtgct ttgacgatgt tgctgccgat aaagccggcc gcgcctgtta
    20461 cgatgatggt catattcggt ttcctttgtt ttgagtaaaa ttaataaact tagttacaag
    20521 aaatcatcga tattgtcctg aaacacaaat ttgcgatgat attccaaata tttttctctt
    20581 ccttcgggat atattctcat cttaattttt tgcatttgga ttgatattaa gtttttccca
    20641 tgtgtaacgg cttagtcgag taccgcaaat caaacgacca tcatccaaga aagtaatata
    20701 accttgatcg aacaaccaat cataagtcgg cgttaaagct aacccattta aataatctaa
    20761 tgcctctttc tcatttttct cagtaataca aaccatataa ggtttaatat gactagccct
    20821 taacaaaatc tcatctgtaa ttaatgtaaa tggacattgc gacatatgat ttataacatc
    20881 cttgcgatat ttctgctgcc cttttctact tttctgttta atgatttctt gctctacgaa
    20941 ttgattaaat tctgaagccg gtatttctaa tttttctctt gataagagtt gcgggtgcac
    21001 gatagagcga aattgataat ccaaaaatat tcggaaataa aataatggtt ctgaatcttc
    21061 tatatctttg acaggcaata gtttaagaat tgataaataa ctaattttgg gcaatacaat
    21121 cttacgccaa attgaccata tatcatcctc cgaacggata taacccctac ggtaaccttt
    21181 agaattttcc aaatggtctg atacatcatg gattgaaaag aaaatatttt ctgtaggcag
    21241 attattaata cttaaaacgt aatcatcata aaaccttgaa atatcatgat gatatttctg
    21301 ttcttgtttg tgatattcaa cttttgcatc aaccaaataa cgctgtaaat ttttcttatt
    21361 aaaaaaacaa tttttctgaa tgaaaggata ttctaccctc cttccatttt ttgtctcagt
    21421 aatcccccaa ttttcatact caaaaaatgt acttagtttt cgaccactat catcaatata
    21481 actacctaca tatttgcgtg cttccccatt accaccaaaa acagataact tgttattcct
    21541 gtgaataaaa ctatcttccg cacgtaaatt ttgaatacta tcgacaataa aatactctat
    21601 gttttcaata gtaattcttt gaaaatccgg cattttactc tccataaagc gtaatatcca
    21661 tttgttttaa tatagcgatt aatacatcaa caacaatact gtttcccgct tggcgataca
    21721 tttgagtatc actaactaaa attttaaaat catctctaaa acccattagc cgcaaacatt
    21781 ccctaggtgt taatttacga atacgtccac gattatgagt aacataatta tcaaccccgg
    21841 cacgatgcat tttatgcata gtttgcaata atgggcgagc tacaggcaaa tcagtttccg
    21901 tactggtttt aaaatttttt gttcctcctg ccaaaacata atttttgatt ttttcagaaa
    21961 gataatattt ttcctcaacg ttatttacat caaaaataaa gtcatcaaat tcagattcgc
    22021 gggctgcctg aaaaataaaa tcaccatgcc aattaaattg ttgatttgct ttttggcata
    22081 aggcaatttc tccattaatc tgtgtataac gtttttgtct atttttagaa ctggtaacaa
    22141 atttcacacc cttttcacgt aaaaaatatt tgctatcagt atagtcctcc aaaaaatctt
    22201 gcatagtatg ttctaattca atcttttcag gaaactgaaa accatttata ggaggggtat
    22261 gaaagccaac aacaaaaata cgctcacgat gttgaggaat cccataatcc ttactattca
    22321 ttatttggaa atataagtca taaccaagtg aataaaaaac actttttaca actttccaag
    22381 tttttccatt atcatgatta agcaagccct ttacattttc ataaataaat atctttggtt
    22441 ggacttcatc cacaacgcga gcaaattcat agaataatgt tcctcgtgta tcttctaatc
    22501 ctgcacgttt gccaaccatg gaaaatgctt ggcaaggact gcctccaact aaaatatcaa
    22561 cttgatttct aaactttctc gcatcaaatt gagtaatgtc gttatgccaa aaatcttcat
    22621 ttaatttata gtttccaaga taactttttt taacgtatgg atcaatatct cctgaaaaaa
    22681 caatggtatg gtttaaattt aatcggtgga atgcctgttc aaccgcacca atcccgctga
    22741 atacggttgc taatctaata tgtgaatcag gtttaagaaa agttttagat ttccaacctt
    22801 gttgactggg aaagagcaaa gttttttgta atcgagtatc gtgtgtctgt gccattgtcg
    22861 aaatagtcat acttatatcg ttctgtttat cttatcaata tgaaaactac attgttgatt
    22921 gccctgacaa tgccttgatc aattcggcaa acgagcaaac cgccgtaccg agtttcgcca
    22981 cgacaacccc ggccgcagta ttggcaaggt acatggcttc gggcatggtg cagcctgccg
    23041 ccaaacccaa gcccattccg gcaatgacgg tgtcgcccgc accggatacg tcgtaaactt
    23101 cttgggcgcg ggtgggctgg taaatcggtt cgccttcgct gaacaaggtc atgccttctt
    23161 cgcttcgggt cagtaaaacg gcggtcaggt cgaggtggcg gcgcaggttt tgcgcttttt
    23221 cggtcagctc gctttcgttt ttccaactgc cgaccacttc tttcaattcg gcgcggttag
    23281 gcgtaatcag agttgcaccg acatattttt cgtaatcgtc gcctttgggg tcgattaaga
    23341 cggttttgcc ggcgtgtttc gcccaatcga tcatatcgga gatatgcgac aggccgcctt
    23401 tgccgtagtc tgaaaaaatg attgcgtcgt attcgggcaa gatttcgcgg tatttctgct
    23461 tgatttgttc caacacttcg cagttgggat gttcttcaaa atcaagacgg ataagctgct
    23521 ggttgcgggc gacgacgcgc agtttgacgg tggtggcgat ttgtttgtcg cgcatcagat
    23581 aggaggcgac gccgtcctgc accatcagcg catcgagcgc gtcggcggct tcgtcgttgc
    23641 cggttacgga caacagccct gccctgccgc ccaacgaagc gatgttgcgc gcgacatttg
    23701 ccgctccgcc cgcgcgttgg tcgattcgtc cgattttcgc caccggcacg ggggcttcgg
    23761 gcgaaatacg ggacacatcg ccgaaccaat agcggtcgag catcacgtcg ccgacaacca
    23821 ggactttggc ttgcgcgaaa cgggatttga gggtttcttg ttggaacttg gcggacatat
    23881 tgcctccgtt gtattttttc agacggcctc agcctgtaag gccgtctgaa aatcagtttg
    23941 cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc taccggatcg gcagcttggg
    24001 ttacaggacg acccattacc aaataagtcg aaccggcagc caaggcttcg gccggtgtca
    24061 tgatgcggcg ctggtcatca ttattgccgg caacgtccaa gcggatgccg ggcgtgacca
    24121 agacaaaatc ctgtcccaat tcgcggcgca gcggcgcggc ttcttgggcg gaacagacca
    24181 cgccgtccaa gcccgaactt tgcgccagtt ttgccaagcg gatgacttgt tcttcagggg
    24241 cggtgttcaa accgatttcc gccaaatcgc tttgttccat gctggtcaac acggttacgc
    24301 cgattaagag cggcttcgtg ccgtatccgg caacggcttc tgctgcggct tccatcatac
    24361 ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc catatcggca gcgactttgc
    24421 aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa atcgaggaaa agtttgaaac
    24481 cttgattgat taagctttct gccaaattgc gtcccgtcgc ggtaaacagc tctttgccga
    24541 ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa tccgagcgtg tctttttcgt
    24601 tggaaaaatc aagggcgacg atgacggggg tgcgttgttg cggagtttgg aagtcggaga
    24661 ttaaaggatt catggtgttt tcgtttcggg ttttcagacg acctttatcg tctttttcgc
    24721 agtatagtgg attaacttta aaccggtacg gcgttgtctc gccttagttc aaagagaacg
    24781 cttctctaag gtgctgaagc acaaagtgaa tcggttccgt actatctgta ctgcctgcgg
    24841 cttcgtcgcc ttgtcctgat ttttgttaat ccactataac aacgtaaatt caaaccgtag
    24901 gtcgggcatt gatgtccgac ctacgcggac tcaagccccc gacaaactgt ttcggataaa
    24961 tttgaaccgg tacatcagcc gtcattcccg cgcaggcggg aatccattga taaaactcag
    25021 ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg gattcccgcc tgcgcgggaa
    25081 tgacgccaaa tgattatttt tcggttgcaa cacgctgttt atcaaaaaca ggtcgtctga
    25141 aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt gggtacgacc ttttcgattt
    25201 atttgcccaa tgcgcccaat acttcggctt tcacggcttc taccggctga gtgccgtcaa
    25261 ctttgatgta tttaggcgcg tgttcgcctt ccagtttgct gtaaaaatca accaaaactt
    25321 cggtttgctc gtggtaaacg gcaaggcgtt ttttcacggt ttcttctttg tcgtcgtcgc
    25381 gctgaatcaa atcttctccg gttacgtcgt ctttgccttc aactttgggc gggttgtagg
    25441 taacgtggta agtacggccg gaagccaaat gcacgcggcg gccgctcatg cggtcgacaa
    25501 tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa atccacgcct gcttcaacca
    25561 tcgcttcggc ctgtgccagc gtgcgcggga aaccgtcgaa caggaaaccg tttttgcagt
    25621 cgtcttgcgc gatgcgttct ttgaccatgc cgataatgat gtcgtcgcgc accaagccgc
    25681 cttcgtcaat gatttttttc gcttccaaac ccaagggcgt gccagcctta atcgcggcgc
    25741 ggagcatgtc gccggtagag atttgcggaa tgccgaacgc ggcggtgatg aattgcgcct
    25801 gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc tttcatgaag tgatcctttg
    25861 aaatgtgttt attggaatca aacagatggt ttacgattct acacggcttg gcacaatagt
    25921 tcaaaaacca actgcacaaa ctgccatttg caagcaaatg ttttgcatac ctttgccgag
    25981 tctgatattc acaagccggc aactttttgg gacaatcccc aaaatttcaa aacatcacac
    26041 cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt tttggctacc aatatcgctg
    26101 ttttggtcgt aatcaacatt gttttggcgg ttttgggcat caacagccgg ggcggcacgg
    26161 gcagcctgtt ggcgtattcc gccgtcgtcg gcttcactgg ttcgattatt tcgctgctga
    26221 tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt tatcgacacg ccgcgcaccg
    26281 aagaagaagc ctggcttttg aacactgtcg aagcccaagc gcggcaatgg aacctgaaaa
    26341 cgcccgaagt cgccatctac cactcccccg aacccaatgc ctttgccacg ggcgcatcga
    26401 gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga ccatatgacg cgtgacgaag
    26461 tggaagccgt attggcgcac gaaatggcac acgtcggcaa cggcgatatg gttacgctga
    26521 cgctgattca aggcgtggtc aatacctttg tcgtgttcct gtcgcgcatt attgccaacc
    26581 tgattgcccg aaacaacgac ggcagccagt cccagggaac ttatttcctg gtcagcatgg
    26641 tattccaaat cctgttcggc ttccttgcca gcttaattgt catgtggttc agccgacaac
    26701 gcgaataccg cgccgacgcg ggcgcggcaa aactggtcgg cgcgccgaaa atgatttccg
    26761 ccctgcaaag gcttaaaggc aacccggtcg atttgcccga agaaatgaac gcaatgggca
    26821 tcgccggaga tacgcgcgac tccctgctca gcacccaccc ttcgctggac aaccgaatcg
    26881 cccgcctcaa atcgctctga gccgatttga aacaacaaaa aaaccgtacg cgcaagcagt
    26941 acggtttttt gtatatgctg tctgaaaaca tcaggtttcc ataccgacac caaccgtatc
    27001 catttccgca ccggatacgc cgcattgata taccctgata aaccgtatcc ggacagaaaa
    27061 tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gaaaacgatt
    27121 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttatactg tctgcggctt
    27181 cgtcgccttg tcctgatttt tgttaatccc ctatatcaga taaaacagcg gtaaaattgc
    27241 cgtctgaaac caactctgcg ccatacggga cacgctctgc acgtctattt gcctccgata
    27301 tatcctccct actgtaggaa cgtctgccga tttgcacgaa atcggcacct ctagcaccct
    27361 tcaggatggc ggacgttatc cgttcttggg attctgcaag acggtcgacg gcattataca
    27421 gacgctcggt ctgataccct tccgaagcaa cctgcttcag gtattccaaa taaccgtaac
    27481 ccaacgcccc tccgacaagc agggtcgtca acgctaccag cgttgccgaa cggattttag
    27541 ggggcatatc ttgaagcata tctctaccga accgaaccaa agtctcttta aagtcggact
    27601 ctacacgcgt agaaccctcg gaaatcttga agttcaactc atactgcttc cgtttgtccg
    27661 ctgtcaaccg cttgaggttg tcatccccgt attggacggt tgacacaacc ctgtacagac
    27721 tgtcctgtat ttcgaccagc gtctgtgcaa agttggcggt cataactgga ttcccaaccc
    27781 ttgccgccga ttctgatact caaaacggta tccaggcgtt ccagcagggc ttcgggagac
    27841 agcagtgcgt attcttcaat attttccata ggttgattat agcagaacct tatacctgtt
    27901 acagacaagg ttgtcgggat ttgaacgtcc gtcgagcttg gttcgtatag agccgtaggg
    27961 ggtgtttagg gctttggcgg cagcttgtac gctggggaac gtgagtgttt cgcctgtggc
    28021 ggtgtcgagg atggagacag gtttttggtt ttggggaggc gtattttcct tcattttaag
    28081 tttcccgcac tccggacacc catactttga ctttatagct gtgtcgtaat acaaaagttg
    28141 gacaaccccg tgcgtaggac atctaacact ggaaggttta taaatgccac tgaactccaa
    28201 caattctaaa tgtggaaatt tctcttgaaa gcgtgatttg gcggtatcga aagtcattcg
    28261 cttacccata atttgtctcc ataggttatg tgcaaatgga ttacacaacc aaaatataga
    28321 aattttcaaa ttttttgaga cctacctgaa atcctgcgca agcacaatgt aaagaaaaat
    28381 ggtatacgtc tttactaaat ctttacataa attaactcta accgggattt gaacgtccgt
    28441 cgagcttggt tcgtaggatt tagggtgttg tgaaccttac ctatcgtacc caagcacttt
    28501 gtacctgttt gaaataaggt tgtcgggcga cgttcgaccc ttgagcctgt gattgatttg
    28561 ttggaaatgt acacctaaag cagcacctgc ggcagtaacg cttgggaacg tgagtgtttc
    28621 gttggtcgtt gtgtcgagga tgaggagggg cttccctaca tttggaggtg tttgggtttt
    28681 tgacatctta gcacactcag gacacccgta ctttgacttt ataagggcat taaatgtcga
    28741 ataacgaacg ttcccgtgca taggacaatt aacggttact gggtaattca caccgtcaaa
    28801 gtcggttaga gagatattag ggaatatatc agtaagttca gaagctgctt ggttgaaaga
    28861 tttggccatt tgagactcca aaattgattg tcaaatggat ttaatagcaa atttataaaa
    28921 atgtcaaatt tttcgagaag cacctgatag tggcgagggg tatacactgt taaaaagtgt
    28981 atacgtcatt tagtgcagat gtgaacctta tacctccctg caagtaaagt ctctgtgtga
    29041 acccgacctt ttatacggtc agtgatagct gctgggttta tacccaatga ccttgcagca
    29101 gatgctcttg aggggaatgt aagtgtttcg ccagttacag tatcctccaa tactgtattt
    29161 ttagataatt tatctattgt atcttctgga attttgtggg aattgacacc ataagatgca
    29221 cattttggac acccactttt agagtccaac atacttttga atgtagagca agtaaccctc
    29281 ccgtgtatag gacagacgat taccgtcggg taacgaacgc cattgaactc cacaagattc
    29341 aaatgtggaa atttagaggt tagcttagaa actgcttgag taaatgtaag tgccatagtg
    29401 gttgtgaact tactttacca acctataacg atacctaatc aatcggtcaa acggagtgcg
    29461 tccgcttaaa cgtacagcta aagtgctgtt ggagcaaccg ataaatttac tggcagccag
    29521 tgtacttgca aactcgtaga tttcccctgt ctctgtgtcc tccaacctaa tggcaacggc
    29581 gtttggagag ggcttttcgg gatttcgagt acattcagga catcctgttg aggatttcat
    29641 ggcagcttgt agattcccgt aaacaaccga cccatgcaaa gggcattgga ttgttaccgg
    29701 ttcgtagttt ttagtgaact tcaaaaccgt cttatcagga aatttactgg aaaaccttaa
    29761 ctgaacatct tgtagcgaga gtgattttgg cattatagac ctttctaaaa tgaaagcatt
    29821 atacaaccaa aatatagaaa ttttcaaatt ttctctgaac gcactggtat ccgatggagc
    29881 taaatgtaaa gaaaaatggt atacgtcttt actaaatctt tacacatttt aactgtatag
    29941 tgtttgacaa acaacaggca taggcgcata atgcacccgt agttagatag atagtaagga
    30001 gataaccaat gacttagcac ctgccccctc tctctcgcgc accccgcccc tcgcttgcgg
    30061 ctaatgggct ttgcgcgaca tatacacttt catctcctgc atggcttaac tgcctatgca
    30121 atagtacaca catcaacaaa tcaggctggc acgtcttggc attggctgac gtaccattgt
    30181 agcccgaaat gttgataccc tactatttat gaccaacggc ggattagggc ttcagacggc
    30241 aattttactg caaaacctta tgcgaacgga tgatgcagca caatcgtttc ttcgcggtcg
    30301 gggccggtgg agacgatggc gaccggcgca ccgcagactt cttcaatccg tttcaaatat
    30361 gctttggcat tggcaggtaa tgcgtcgtag cttttcacgc cgaaagtgga ttcacgccag
    30421 ccgggcatgg tttcgtaaat cggcttgcag gtttccaccg catcggaacc gcaaggcagg
    30481 atgtcggttt tgccgccgtc gggcaattca tagccgacgc agatattgat ggtttcaacg
    30541 ccgtccatta catcgagttt agtaatacac ataccggaaa tgccgttgat ttggatggag
    30601 cgtttcaatg cggcggcatc aaaccagccg cagcggcgtg cgcgtccggt taccgaaccg
    30661 aattcgtgtc cgcgttctgc caaacctgcg ccgacttcgt cgaacaattc ggtcgggaac
    30721 gggcccgaac cgacgcgcgt ggtataggct ttgacgatgc ccaaaacata atccagcatt
    30781 tgaggaccta cgcccgcgcc tgcggaagcg gctcccgcca gacagttgga cgaggtaacg
    30841 aagggataag tgccgtagtc gatgtccaac aacgcacctt gcgcgccttc aaacagcagt
    30901 ttttcgccgt ttttgttttt ctcgttcaac acgcgggaca cgtcggtaat catcggcgca
    30961 atgcgcggcg cgactttttc gataaccgcc atcacgtctt ccgctttaac cggctcggca
    31021 ttgtgcagat gttgcagttg gacattgtaa taggcaagga cggcatccag tttttcacgc
    31081 agtttttcag gatgcagcaa atcggcggcg cgaatggcgc ggcgtgccac tttgtcttcg
    31141 taggcagggc cgatgccgcg gccggtcgtg ccgattttgc ctttgccgcg cgatgcttcg
    31201 cgggcttggt cgagcgcgat gtggtaaggc aggatcagcg ggcaggtcgg cgcgattttc
    31261 agacggcctt cgacgttttt cacgcctgcc gcgttcaact cgtcgatttc gcccaacagg
    31321 gcttcggggg agacgacaac gcccgaaccg atgaagcagt ccaaactttc atgcaggatg
    31381 ccgctcggaa tcaggcgcaa aatggttttt ttgccgccga caaccaaggt atggcccgca
    31441 ttgtggccgc cttggaagcg caccacgccg ccggcttctt ccgccagcca gtcaacgatt
    31501 ttacctttac cctcgtcgcc ccactgtgcg ccgattacta caacattttt agccatagcc
    31561 atataaccta tcgatattaa aaattattgc ggaaatcccc aaaccggtgc ggctttgcgc
    31621 cccgccacga tttgcctttc atccgccatg aacgggtatt tagcgtttca cgacctgcca
    31681 aacgccgtcc gtctttttca gacggcctgc aagctcttcc gaaacattgt gtccgatacc
    31741 gtagtcaatc acgacacact gcccttgttc acgcaaggct tcgactgctt cgcgcgccgc
    31801 ttcggcatct tccgcatcga ccaacacggc gggctgccgt tcgatggtgg gcaaacgccc
    31861 gataaagctg cgcaagtcga aactgaatcc cgttgccggg cgcgccctgc cgaaatatcc
    31921 gcccaatccg tcataacgcc cgccgcgcgc gaccgcgtcg tggaaatcgg cggcataggc
    31981 ggcatacagc aagcccgtgt ggtaattgtc gacacgcagc tcggacaagt cgatatggat
    32041 ttcacaatcg gggaatgcgt cgcacaccgc ctgcaattcg cccaacgcgc cgccgaccgc
    32101 cgacaaatcc ggcaaccgtc cgcgcgcgtc ggacaacact tcacgcccgc cgtacaggcg
    32161 cggcaacagc gagaatgctt ttgcccacat gccgtccagc ttccaagcct tgacctgcgc
    32221 ttcgaccgcc ccggtatctt tatcctgcat caaggcaagc agcgttgcgg actgccccgc
    32281 atccaaatgt gccgcatcgg acaaggcgcg aaatatgccg atatgcccca gcgaaagcag
    32341 cactttgccc atatcggcaa ttttcatgct tttcagcata aggtctatca gctcgatgtc
    32401 gccacggatg tcggcaaaac cgtacatttc cgcccctgcc tgcaagggtt cgcgcatatt
    32461 cagcagaccg tcgggctgcg cgtgcaacac cggaccggca taacacaacc gattaatccc
    32521 ttggttggcg gataaaagat gggcatcgat acgcgccacc tgcggcgtga tgtcggcgcg
    32581 tatgcccaac tgcctgccgc tgagcctgtc cgttaccaaa atggttttca gggaaagccc
    32641 cgcatcgata tgcgtcagca gggaatgtgc gtactccatc agcggaggct gtaccagttc
    32701 ataaccgtgt acgcggaaca gtgccaacaa ctgctccctc gcgctttcaa gctgccgcgc
    32761 gttcgtgggc agtacgtcgg cgatatgttc cggaagctgc catgtctgca tgagaatctg
    32821 ccttctattt tatcatgtaa atacaaaagg ctgcccgatc cgcaatcaga cggcatcttt
    32881 gccgcaacgc atcacgccgc tgtcaaaacg gcgatgcttc cttccgattc agaatccgaa
    32941 cagcccctgt ccgacggctc aatgccgtca agtcgttaaa accaaacttt accataaaat
    33001 acacacaatc tgaatatact tttataccct gtatccgccg tctgacggca cggcattttt
    33061 ttactatata atgtgcacca ttatttctca tcacttcctg ctgccgtttt ctgcttcaga
    33121 cggcattttt gttgattgaa cacttatgct gctcgacctc aaccgctttt ccttttccgt
    33181 cttcctgaaa gaagtccgcc tgctgaccgc tcttgccctg cccatgctgt tggcgcaggt
    33241 cgcgcaggtg ggcatcggtt ttatcgatac tgtgatggcg ggtggtgcag gcaaggaaga
    33301 cttggcggcg gtggctttgg gcagcagcgc gtttgccacg gtttatatta cctttatggg
    33361 cattatggcg gcgctgaacc cgatgattgc ccagctttac ggcgcgggta aaaccgacga
    33421 agtgggcgaa acggggcggc aggggatttg gttcgggctg tttttgggcg tgttcggcat
    33481 ggtcttgatg tgggcggcga ttacgccgtt ccgcaactgg ctgaccttga gcgattatgt
    33541 ggaaggcaca atggcgcagt atatgctgtt caccagcttg gcgatgccgg cggcaatggt
    33601 acaccgcgca ctgcacgcct acgcctccag cctgaaccgc ccgcgcctga ttatgttggt
    33661 cagctttgcg gcgtttgtgt tgaacgtgcc gctgaactat attttcgttt acggcaaatt
    33721 cggtatgccc gctttgggcg gcgcaggctg cggactggcg acgatggcgg tgttttggtt
    33781 cagcgcgctg gcattgtgga tttatatcgc caaggaaaat ttcttccgcc cattcggact
    33841 gacggcgaaa ttcggcaaac cggattgggc ggtgttcaaa cagatttgga aaatcggcgc
    33901 acccatcggg ctgtcttatt ttttggaagc cagcgcgttt tcgtttatcg tgtttttgat
    33961 tgcgcctttc ggcgaggatt atgtggcggc gcagcaggtc ggcatcagtt tgtcggggat
    34021 tctctatatg attccgcaaa gcgtcggctc ggcggggacg gtgcgcatcg gcttttcgct
    34081 tgggcggcgc gaattttcgc gggcgcgtta tatttcgggc gtgtcactgg tgttaggatg
    34141 gatgctcgcc gtgattaccg tgctttcctt ggtattattc cgttcgccgc tggtaagtat
    34201 gtacaacaat gatccggcgg ttttaagcat cgccgccacc gtcttactgt tcgccggctt
    34261 gttccaaccg gcagacttca cccaatgtat cgcctcctac gccttgcgcg gctacaaagt
    34321 tacaaaggtg ccgatgttca tccacgccgc cgccttttgg ggctgcggtc tgctgccggg
    34381 ctacctgctc gcctaccgtt tcgatatggg catttacggc ttctggacgg cattgattgc
    34441 ctcgctcacc atcgccgcca tcgccttggt gtggtgcttg gaattgtgca gtagggagat
    34501 ggtcagatcg cataaggccg tctgaaaact ccgccgatta aaaatatagt ggattaacaa
    34561 aaaccagtac ggcgttacct cgccttgccg tactatttgt actgtctgcg gcttcgttgc
    34621 cttgtcctga tttttgttaa tccactatag aaacgatggg ctgaagcccg ccactggcaa
    34681 tactcatttt aaagttcaaa cccatgcaac ccatccgata ccgaaccgac cttaccccct
    34741 acaacacttt cggcctgcgc gcccaagccc gagcctttat tgcgcttgaa cacgccgacg
    34801 ggttgcgcga catcgtccgg ctacccgagt tcaaccgaga tactgtttta tggctgggcg
    34861 gcggcagcaa tatccttttg atggaggact acgccggtct ggtcgtacat atggaaaaca
    34921 aaggcatacg cgaaattgct cgttcagacg gcatggttct gattgaagcg caggcgggcg
    34981 aaatctggca cgattttgtc ctgcacgccg tcgcgctggg tttgaacggt ttggaaaacc
    35041 tgagcctgat tccgggtacg gtcggcgcgt cgcccgtgca gaacatcggc gcatacggcg
    35101 tagaggcgaa agatgtgatt cacagcgtgc gctgcttcga tttggatacg gaaacctttg
    35161 taaccctttc caatgccgac tgccgcttcg cctaccgcga aagcctgttc aagcaggaag
    35221 gtaaagggcg ttatgtgatt gtttcggtcg tatttgcatt aaaaacgcat tttgtgccga
    35281 acttgggtta cggcgatttg gcggccgccg ttgccgaact gagcgcgggc agggtgccga
    35341 cggcgaaaga tgtttccgat gcagtgtgtg caatccgcaa cagtaaactt cctaatccta
    35401 acgtgcttgg caatgtcggc agtttcttta aaaaccccgt cgtcagcgca gaaaaagccg
    35461 ccaccttgtt gcagcggcat cctgatatgc cgcgctatcc gcagcccgac ggttcggtca
    35521 aactcgccgc cggctggctg atcgaccaat gtcgtctgaa aggctttcaa atcggcggcg
    35581 cggcggtaca tgacaggcag gctttggtct tagtgaacaa aaacaatgcc tcggcaaacg
    35641 atgtccggca attggcgcaa cacatcaaat ttacagtatt tgctcggttt caggtagaat
    35701 tgcacgccga acccaattgg ctgcctgctt cgttcagcct gtaaatccaa ggagtatgcc
    35761 cgtgacccgc cccgccaaaa tcaatactta cacacgcatc atcgacgcca gccttgcgct
    35821 tttcaacgag gaaggcgagc gcaacatcag caccaaccat attgccgccc acttgggcat
    35881 cagtccgggc aacctctatt accacttccg caacaaagac gaaatcatcg tccaactgtt
    35941 caaacgttac agcgaagccc tgctggaata cctcaacgaa gccgtgttgc cgtctgatgt
    36001 ggaagactcc atcaattata tggcgggcat ttacgacgtg atgtgggaat accgcttcct
    36061 attcagcgac gtgaacaccc tgcttgcacg cagtgccgaa ctattgggcg aacacaatac
    36121 cttcacccaa gccaaagtct ccccgctctt ggtcaacctg ctgacccagc tcaacggtct
    36181 gaacatcatc caagccgacc aaaccgccat gaacgacctc gccgtcaata tgtggatgat
    36241 cacgaaatac tggttcgact tcgacagctc cctgcgcggt cgtaccaagc tgaccgaaga
    36301 ctccaaagca cgcggcatcc gccgcacctt aagcctcctt cgtccttatc ttttgcccga
    36361 acaccgcaag aaatacgacc ggaaaatcgg caacggcaac ccgtaaaccc acagcgtccg
    36421 aatcagcctt cagacggcac ttacccgacg gcatcagcga atgcgcgcag tccccccgcc
    36481 ccatatctcc atcacaggcc taggttacct cggcctgccg ctggcacaaa agttttacca
    36541 acacggcagc cgcgtcgccg ccataaaacg cagcctgact tcggacgaca tcaacctgcc
    36601 catacacctc gataccatcg acctcaatca agacagcgcg tttcaaagcg cgaaccttgc
    36661 ccgagataca agcttttggc ggcaccatgc caacaaaccc gtttggttct gcctcttgcc
    36721 gccatcatcg ctgacacatt acgccgatac cgtcaaacaa tgggcagaac ttgcccgggc
    36781 gtgcaacgtg caacacctga ttttcacaag cagtaccagc gtttacggcg atacagcgcg
    36841 cgaatgcgac gaaatcgccc tacccgatcc gcaaaccgag tccgcccgcc aaatcctcgc
    36901 cgccgaacaa cacctgctcg acagcggcgt tccgaacatc gacatcctgc ggctgggcgg
    36961 gctttattgc gccgaacgcc atcccgtcag ccgccttgtt caaaagcaaa acatcccggg
    37021 cggcaaccgc cccatcaaca tcgtccaccg taatatcgcc gtcgaaagcc tgtttcagac
    37081 ggcatttaac cccagcggca ggcggctgaa aaacattatc gaaccgcgcc acccgacacg
    37141 ccgcgaattt tttacggaag aagccgccaa actcggcctg cccgcgccgg atttcgcacc
    37201 cgacgacagc gtgggcaaaa tcatccgtac cgtttgcgat aacggcttaa gcctgtaaaa
    37261 taagcggcaa cagcaacaaa caggcattct ccgccccaat cagaaaatat gtccgccttc
    37321 ctccccatca tcaaccgcct gattctgcaa agcccggaca gccgctcgga acttgccgcc
    37381 tttgcaggca aaacactgac cctgaacatt gccgggctga aactggcggg acgcatcacg
    37441 gaagacggtt tgctctcggc gggaaacggc tttgcagaca ccgaaatcac cttccgcaac
    37501 agcgcggtac agaaaatcct ccaaggcggc gaacccgggg cgggcgacat cgggctcgaa
    37561 ggcgacctca tcctcggcat cgcggtactg tccctgctcg gcagcctgcg ttcccgcgca
    37621 tcggacgaat tggcacggat tttcggcacg caggcagaca tcggcagccg tgccgccgac
    37681 atcggacacg gcatcaaaca aatcggcagg aacatcgccg aacaaatcgg cggattttcc
    37741 cgcgaatccg agtccgcaaa catcggcaac gaagcccttg ccgactgcct cgacgaaata
    37801 agcagactgc gcgacggcgt ggaacgcctc aacgaacgcc tcgaccggct cgaacgcgac
    37861 atttggatag actaaccttc agacggcatc cgacatgaac agcccttttc acaacatcgg
    37921 catcgtaacc cgccccaaca cgcccgacat ccaagacacc gcacacacgc tgattacctt
    37981 tttgaagcag cacggcttta ccgtctatct cgacgaagtc ggcataaagg aaggctgcat
    38041 ctatacccaa gacaccgtcg gctgccatat cgtcaacaag accgaactgg ggcaatactg
    38101 cgacctggtc gccgttttag gcggagacgg cacctttctc tccgtcgccc gcgaaatcgc
    38161 cctgcgcgcc gttccgatta tcggcatcaa ccaagggcat ttgggcttcc tgacccaaat
    38221 tccccgcgaa tatatgacgg acaagctatt gcccgtttta gaagggaaat accttgccga
    38281 agagcgcatc ctgattgagg ccgcactcat ccgcgaaggc aaaaccgccg aacgcgccat
    38341 cgccctcaac gatgccgtcc tctcccgtgg cggtgccgga cagatgattg agtttgaagt
    38401 cttcgtcaat cgggaattcg tctataccca gcgttcggac gggctgattg tctccacccc
    38461 caccggatcg accgcctatt cgcttgccgc cggcggcccc atcatgcagg caggattgca
    38521 cgccttcacg ctcgtcccca tctgcccgca atccatgacc aaccgcccca tcgccattcc
    38581 agacacgtcc gaaatcgaaa tcctcgttac ccaaggcggc gacgcgcgcg tccatttcga
    38641 cggtcaaacc catatcgacg tgcaaaacct cgaccgcatc accatccgcc gctaccgaaa
    38701 ccccttacgc atcctacatc cgaccgacta ccaatatttc aaaaccctgc gccaaaaact
    38761 gcactggggc gaacaattgg tctgaaccga cccttaccgg aaaacaccta tgaatccaaa
    38821 aactcccccc gaccatacag aagatgccgt gcgcctgtcc aaacgcatgg cgcaactggg
    38881 gctttgttcg cgccgcgaag ccgacggcta tatcgaacag ggttgggtaa cggtcaacgg
    38941 caaaaccgcc gtactcggtc agaaagtttc gccggcagac cgtatcgaac taaacaagaa
    39001 agcccacgaa cagcaggcgg cacgcattac catcctgctg aacaaacccg tcggctatgt
    39061 cagcgcacaa gcggaaaaag gctataaatc cgccgccgaa ctgattacct ctgaaaaccg
    39121 ctgggaaagt gacaccagcc gcatccgttt cgatccgaaa cacaaaatcg gtcttgcccc
    39181 cgccggcagg ctcgatatcg attcagtcgg attactggtg ttgacgcagg acggccgtat
    39241 cgccaagcag cttatcggcg aaaacagcgg cagtgaaaaa gaatatttgg tgcgcgtgcg
    39301 cggaaaattg gacgaaaaag gacttgcctt actgaatcac ggattgagtt tggacggtga
    39361 gaaactgcgt cccgcccaag tagaatggca aaacgaagac caactgcgtt tcgtattgaa
    39421 acaaggtaaa aagcggcaaa tccgccgtat gtgcgaactg gtcggactgc gcgtcgtcgg
    39481 gctgaaacgc atccgcatgg gtaaggtcaa gctcggcagg ctgccgcccg gcaaatggcg
    39541 ttatcttgct cccggcgaat cgttttaaat aagcattaaa aaatgccgtc tgaacgtcct
    39601 tacgttttca gacggcattg ccttatgccc aaataacggt ttcttccaag ggcctgcgcg
    39661 ctttcgtggc gatttcctga acgcggtagc cgaatgtcgc ggcaacggac acgccccatt
    39721 ctgccgcatc gaacaaaccg aactgcccgg acaatacgcg ttccatctcg gcatagttga
    39781 aaccttccac cgggcaggaa tcgatacctg ccatcgccgc acccgtcatc atgttgccca
    39841 acgcgatata ggtctggcgg caacaccagt caaacaaggc gcgcgaatcg tccaaaatct
    39901 tgatgtcgtc ggcttgaaac gcctgatacc tcgccaaaga ttttgccatg gcatccggtt
    39961 cggtaacgcc gcgccgtttg aggctttcca acataaacgg gctgtcgaag cgggcatttt
    40021 tctttgccaa aaacaccacc aaatgactgg cggtatccaa agcatccgcc ataccccaag
    40081 aaaacggctt gattgcctgt cggatttcgg ggttttgaac cacaacaaac tgccaaggct
    40141 ccgaaccgac cgaactgggc gacaaacgcc cgagttctaa aataaactga aaatcctcgg
    40201 cactgatttt acgtgccgca tcgtaatgcc ggcatgattt gcggttttta aatgcggata
    40261 gaacctgctc tttgcttaat actgccatcg cacatccttt cattacttgt attgcaaatt
    40321 gatttcaaac cgatacggca ctgccgcacc atacaatccg tcctcgccat caagcttcat
    40381 ccggacttta cccatctctc tacggctggg aaaacaaagc ggcggcagac tcgggaataa
    40441 cgccccaatg cctttcagac ggcatttggg catcattgca aacgcgccta cgggtaggcg
    40501 atataggcat aagccgaatg gttgtggata gactcgaaat tctcgctctc gacaacgaaa
    40561 ctcttgatgc gtttgtcggc gatcagcgaa gtagcgacat cgcgcaccat atcttccacg
    40621 aatttcgggt tttcgtaggc tttttcggta acgtattttt catcggggcg tttgagcagg
    40681 ccgtagagtt ggcagcttgc ctgtgcctcc acataatcga tgacttcctc aataccgact
    40741 tcggcatcgg cagtcaggct gacggtaacg tgcgaacgct gattatgcgc gccgtattgg
    40801 gaaatttctt tggaacacgg gcaaagcgag gttacgggaa tcatgacctt catactgtgg
    40861 ccgtatgccc cgtctttgat ttcgcccgtg agacagacat cataatccag taaggaccga
    40921 ataccggaaa ccggcgcggt tttcttgcgg aaaaacggaa aagaaacgct gattttgccg
    40981 gcgcgggaat ctaaaagcgc gaccatttcg gtagtcagcc tgtgcaattg tgcaaaatcc
    41041 aaggcttcgg catgttgctc catcaatgcg acaaaacgcg acatatgcgt ccccttctgc
    41101 tcagcaggca ggtaaaccgt catggtcaga cgggcaatgg tggactggat gccttctgca
    41161 gtttgcaggg taatcggaaa gcgcaggtct ttgataccga cctgattgat cggcagattg
    41221 cgcaaatctc ggctggattg cacgtctgca atagtgttca tgagttgttt ttccttaata
    41281 ttcatgtaat tgttgagatg tgtgcatggt atggcgtttc cgccggggaa tataccgtca
    41341 atctgcaaat gcgagattat atcactcgct tttagcagct gcattgattg tttctatttt
    41401 tcagatagtg ataaaatctt cgattattca catctacata ggcagagacc aaatgaaatt
    41461 cgccacgaaa gccattcatt ccagctacga ttgcgacgaa cacaaccgcg cgctgatgcc
    41521 gccgatttat caaaacagta tgtttgcgtt gcacgagatt ggcgaaaatg tgccttaccg
    41581 ttattcgcgc ctgagcaacc cgacccgtca gattttagaa gacaccgttg ccgatttgga
    41641 acacggtgcg gcaggttttg cgttttccag cggtatggcg ggaattgatg ccgtatggcg
    41701 cactttcctg cgcccgggcg ataccattgt cgccgtcgcc gatatttacg gcggcgctta
    41761 tgatttattg gtcgatgttt atcaaaaatg gggggtgaac gttgtttttg ccgatttagg
    41821 caatccggat aatttggacg aactgcttaa agcgcacaag gtcaaactgg tttggctgga
    41881 aacgccgtcc aatccacttt tacgcttggt agacatcaaa gtccttgccg caaaagccaa
    41941 agcagccggt gcgctggtcg gtatcgacaa cacttttgcc acgccgtatc tgcaacagcc
    42001 gttggatatg ggttgcgatt ttgtattcca ttccgctacc aaatatttgt gcggccattc
    42061 cgacgtgttg atgggcatcg tcgttgccaa aaccaaagaa ctggcgcagc ctttgcacga
    42121 tatgatggtg cataccggcg cggttgccgg cccgctggac tgctggctgg tgttgcgcgg
    42181 catcaaaacg cttgccctgc gtatggaggc acactgcaaa aacgcactcg aaatcgcgcg
    42241 ccgtctggaa gaccatcctg ccattgaaaa agtgttccat cccggcctgc cgtcgcacga
    42301 acattatgaa ctcgcccaag cgcaaatgcc taaaggcatc ggcggcgtgg tgacggttta
    42361 tctcaaaaac gacacgcgcg aagcggcaaa cagcgtgatt aaaaacatga aactggtcaa
    42421 aatggcttcc agcctcggcg gcgtggaaag tttggtcaac cattgctatt cccagtccca
    42481 cagcggcgtg ccgcataatg tgaaaatgga aatgggcatc aaagtcggcc tgctgcgttt
    42541 ctccatcggc atcgaagacg cggacgatat ttggaacgat atttccgccg cactcgatac
    42601 gactttgtaa actgtaaaaa tgccgtctga aaccatggtt tcagacggca tttcaattaa
    42661 cccggccgaa aatcaacgct tcaacatctt tgccgcctca atagcgtaat aggtcaaaat
    42721 cccgtccgca cccgcacgtt tgaatgccag caggctttcc aaaaccactt tgccgccgtc
    42781 cagccagccg ttggcaatcg ctgcctgcaa catcgcgtat tctcccgaaa cctgataggc
    42841 ataagtcggt acgccgaact cgtccttcac gcggcggaca acgtccaaat acggtaaacc
    42901 gggcttcacc atcaccatat ccgcaccttc ccgaatatcg agcgccactt cattcagcgc
    42961 ctcatcggta tttgccggat ccatctggta ggttttcttg tccgccttgc ccaaattgcc
    43021 cgaactgccc accgcatcgc ggaacggccc gtaaaacgcc gaagcatatt tggcggaata
    43081 cgccataatc cgcgtgtgga tatgcccggc atcctccaac gcctcgcgaa tcgcaccgat
    43141 acgcccgtcc atcatatcgg aaggggcaac cacctgtgcg cccgcttcag cgtggcacaa
    43201 agcctgcttg accaaaacct ctacggtttc atcgttcatc acataaccgt tttcgtccgt
    43261 cagcccgtcc tgaccgtgaa ccgtataagg atcgagcgcg acatccgtca taatgcccag
    43321 ttcgggaaac ctctcgcgca gggcgcggac ggctgccggc acgagtcctt cgggattgta
    43381 cgcttcctct gcacgctcgg ttttgtttgc cgtaaccacg ggaaagagtg ccaacatcgg
    43441 aataccgagc tttaccgcct cttccgccgt aaacagcagc ctgtccaaac tttgacgctt
    43501 cacacccggc atagaaggca catcctcctc gcgcgccgac ccctccaata cgaacaccgg
    43561 ataaatcaaa tcatcggcgg tcagcgtgtg ttcgcgcatc aggcggcgtg aaaaatcgtc
    43621 cctgcgcata cggcgcatac gcgaagccgg aacattgcgg taaggaaact gcataagccc
    43681 tccaatcatc ctgtcaacaa ttcaaaccat acggaagccg ccgcaacttg cagacagcat
    43741 cgggcggcaa tcaaaatccg ttttcaccgg aaagggtttc ggcccgccga aatgccgcgc
    43801 cgaaggacgg cgtcccgccc attaaagaac aatagcaaaa caacagcttc aggctgtcag
    43861 ccttcgatat tcttacgcca caataccaaa agtttcccga tatgctgaac aggttgcgcg
    43921 tcaaccgcct cacataaggc agtgcagatt tcgatacgtt cggcacggtc gtcgccgaat
    43981 acgcgcactt taatcagctc atgcgccgtc aatgccgcat cggtttcctt gatgaccgcg
    44041 tccgtcagac cctgctgacc gaccatcaca acaggatgga gatggtgcgc gcgcgctttc
    44101 agttccaaaa tttctttggt gttcaatttg gtatccgtca ttttcctact gcttgaataa
    44161 aaagaataat gcggcattgt acgcgatttg gcacgacatc ggcaaaatta acgtacaata
    44221 cgcggtttcc attccgaccc gcgaaccact atggctgtac gttccaaatc ctcaaaagcg
    44281 tggctgcacg aacacgtcaa cgaccactac gtccatatgg cgcaaaaaga cggctaccgc
    44341 gcccgtgccg catacaaact tttggaaatc aacgacaaag acaaattaat caaacccggc
    44401 acggtacttg ccgacttggg cagcgcgccg ggaagctggt cgcaggttgc cgccaagctg
    44461 acgggttctt ccggagcagt tttcgccttg gacatcctgc ctatggaagc catagggggc
    44521 gtctccttca ttcagggcga cttccgcgag aacgacgtac tggcacaatt cgaaaccttg
    44581 ttggacaacc gcccgctcga ccttgtaatt tgcgatatgg cgcccaatat gtcgggaaac
    44641 gccgtaagcg atcaggcacg cagcttttat ctgtgcgaac tggctttaga cttcgcctcg
    44701 caacacctga aaaccggcgg cagctttttg gtcaaagtct ttcagggtgc aggctatcag
    44761 gaatacatgg cagccatgcg cgaaattttc ggcacggtgc agacgcgcaa acccgaagcc
    44821 tcgcgcaatc gctccagtga gatttattta ttgggcaaaa ataaacgctg acaatacaga
    44881 cggcgtgctt tacaatcatt tccgttttac acctcaatta tggagccttg ctaagtgggg
    44941 aacaccttta aatcaatcct tgtctgggtc gccttgggta tcggcctgat ggctgcgttc
    45001 aacgctttag acggtaaaaa agaagacaac gggcaaatcg aatattctca gttcatccaa
    45061 caggtcaaca acggcgaagt atccggcgtc aacatcgaag gatccgtcgt cagcggctac
    45121 ctgattaagg gcgagcgcac cgacaaaagc accttcttca ccaacgcgcc tttggacgac
    45181 aacctgatta aaacactgct cgacaaaaac gtccgtgtaa aagtaacgcc ggaagaaaaa
    45241 ccgagcgcgc tggctgccct gttttacagc ctgctgcccg tcctgctgct gattggcgcg
    45301 tggttctact ttatgcgtat gcagacgggc ggcggcggaa aaggcggcgc attctcattc
    45361 ggcaaaagcc gcgcccgcct actggacaaa gatgccaaca aagttacctt tgccgatgtc
    45421 gccggctgcg acgaagccaa agaagaagtg caggaaatcg tcgattacct caaagcgccg
    45481 aaccgctatc aaagcctggg cgggcgcgtg ccgcgcggca tcctgctggc gggcagcccg
    45541 ggtacgggta agacgctttt ggcgaaagcg attgcaggcg aagccggcgt gccgttcttc
    45601 agcatttcag gttccgactt tgtcgaaatg ttcgtcggtg tcggtgcaag ccgcgtccgc
    45661 gatatgttcg agcaggcgaa gaaaaacgcc ccctgcatca tctttatcga cgagattgac
    45721 gcagtcggcc gccaacgcgg cgcaggtttg ggcggcggca atgatgagcg cgagcaaaca
    45781 ttaaaccaat tgttggttga aatggacggt tttgagagca atcagactgt aattgtgatt
    45841 gcggcaacca accgccccga cgtactcgat cctgcgctgc aacgccccgg ccgtttcgac
    45901 cgccaagtcg tcgtccccct gccggacatc cgggggcgcg aacagatttt gaacgtccat
    45961 tctaaaaaag tgcctttgga cgaatctgtg gatttattgt ccctcgcgcg cggcacgccg
    46021 ggtttttccg gtgcggattt ggcaaacctg gttaacgaag ccgccctgtt tgccggccgc
    46081 cgcaacaaag tcaaagtcga tcaaagcgat tttgaagacg caaaagacaa aatctatatg
    46141 ggtccggaac gccgcagtat ggtgatgcac gaagacgaaa aacgtgcgac ggcgtatcat
    46201 gagtccggac acgcgattgt tgccgaaagc ctgcccttta ccgaccccgt ccacaaagta
    46261 accattatgc cgcgcggacg tgcgctgggt ctgacttggc agcttccgga gcgcgaccgc
    46321 atcagtatgt ataaagatca gatgttgagc cagctctcca tcctgttcgg cggacggatt
    46381 gccgaagaca tcttcgtcgg acgcatctcc accggcgcat caaacgactt tgaacgcgca
    46441 acccaaatgg cgcgcgaaat ggtaacgcgc tacggcatga gcgacaaaat gggcgtgatg
    46501 gtttatgcgg aaaacgaagg cgaagtcttc ttaggacgca gcgtaacccg ttcgcaaaac
    46561 atttccgaga aaacccagca ggacatcgat gcggaaatcc gccggatttt ggacgagcag
    46621 tatcaggttg cctacaaaat cctcgatgaa aaccgcgaca agatggaaac catgtgcaaa
    46681 gccctgatgg aatgggaaac catcgaccgc gatcaggtac tggaaatcat ggcgggcaaa
    46741 caacccagtc cgcccaagga ttacagccac aacctgcgcg agaatgcgga cgcggcggaa
    46801 gataacgcgc cgcacgctcc gactcgggaa gaaaccgaag cacctgcccc ggcagacacc
    46861 gcttcgacag agtccgagca gcagcctgaa aacaaggctt aacttcccga acaaacggca
    46921 gcccgcaagc tgccgttttc ccatcccgtt ttcagacggc attttccact caatgccgtc
    46981 tgaaacgcca aagccgtatc ggtttttacg aatctgcgat atggcggttt aaattgaaat
    47041 cagaacgggg caaggctgta ccggtttaaa attaaaccgc tataataccg cccttcgaat
    47101 acacgcccgc attggtgatg cccgtgaaaa aagttgaaaa aaacatcttg gtgctgcacg
    47161 gcgcggacaa aatgttcgag ctggtcgaca aggttgagga ttacccgcac tttctgccgt
    47221 ggtacagcaa gaccgaagtc atcgggcgta gcggcaacga actgaaggcg cggctgttta
    47281 tggattatat gcacgttcgc caatcgtttg ccacgcacaa ccgcaacatt ccgggcaggg
    47341 aaatccgtat ggaactgctc gagggtccgt tcaaaacctt acgtggaacg tggaaattta
    47401 tcgatttggg cgacgatatg tgcaaaatcg aattcaattt ggaatacgat ttttccaatg
    47461 ccgttttgtc cgccttaatt tcccccgtct tcaaccacct ttccaccacg ctggtcgaag
    47521 cgttcgtcaa agaggcagac cgccgttatg cttgaaattg agattgtgta cgggctgccc
    47581 gaccgacagg ttttgaaaac catgcagctt gccgagggaa caaccgtccg cgccgccgca
    47641 ctgcaaagcg gtttggacgg catatttgaa gatttaaacc tgcattccgc gcctttgggc
    47701 attttcagca aagccgtcaa agacgacacg ccgctgcgcg acggcgaccg catcgaagtg
    47761 taccgcccgc tgttgatcga ccccaaagaa gcgcgccgca aacgcgttca aaatcaagaa
    47821 gaataaccat gccgtctgaa ccccgtccgg acggcatctg atggaaaacg acatgtcaaa
    47881 caaaatcaaa atggttgtcg gcttgggcaa cccgggcaaa gaatacgaac agacccgcca
    47941 taatgcaggc ttttggttcc tcgacgaact ggcttggaag tggaaggctt cgtttaaaga
    48001 agaaaaaaaa ttcttcggcg aagtcgcccg tgctaccctg cccgacggcg atgtctggct
    48061 gctcaagccg accacgttca tgaaccgttc cggacaggca gttgccgccc ttgcgcagtt
    48121 ttataaaatc aaacccgaag aaatcctcgt cgtccacgac gaactcgaca ttccctgcgg
    48181 acggatcaaa ttcaaactcg gcggcggcaa cggtggacac aacggcttga aagacattca
    48241 ggcacggctc ggcacggccg actattaccg tctgcgcctc ggtatcggcc atccgggcga
    48301 ccgcaacctc gtcgtcggct atgtcctgaa caaacccagt acggaacacc gccgacagat
    48361 tgacgatgcc gtcgccaaat ccctgcaagc catacccgac atccttgccg gcaaatggga
    48421 agaagcaaca cgtttcctgc acagcaaatg acccaatgcc gtctgaagcc ctttcagacg
    48481 gcattcccgc catcagcgag aacccgcact atgtccgaac tcaaccggct catccaaacc
    48541 gaatccgttg ccgtcattga agaaacttta aattttctgc tctacgaatg cagcatagac
    48601 gatgccccct ccgccgaaga aattgccgtt tggcgcgata tgctggccgc acgcggcgga
    48661 aaattcctgc gcctatccaa actatgccag acatggcttg aagaggaaca agcatgaatc
    48721 tgccacgcaa ccgctttatc ctgctctcgg cattgtggtt tgcaggcagc atttactcac
    48781 tgcttttcaa agctgccgaa accgcgccac cgccttttcc gcattttgac aaagtggcgc
    48841 acctcgccct gtttttcgca caaatctggc ttctgaccaa agcattcaga accgacaacc
    48901 gccccatccc ctatcgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat
    48961 gcgcgcaggc atggtttacc gcaacgagaa ccggcagttt gggcgatgtc cttgccgacc
    49021 tgacgggcgc agcccttgcc ctctttaccg cgcgagctgc ctgccgcccg gactaaatcg
    49081 gtttattttc cccaaacagg atgcacctct tcccgatata tcctattttt ccccttacat
    49141 atcaatccca tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca
    49201 gattacaaat atactcgaac cagttgcaga agcggcgatt ccacaaaccg tttcggattg
    49261 cacgacacaa aagaaaaacc gattttgttg ccttaaagga gtattcatga acctgcatgc
    49321 aaaggacaaa acccagcatc ccgaaaatgt cgagctgctc agtgcgcaga agccgattac
    49381 cgactttaag ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca
    49441 catcctgcct tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc
    49501 actttggttt accgaggccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc
    49561 cgtcgtactc ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc
    49621 gattatctac atttttttcg gcggcttcgc gcttgccacc gccctgcata tgcagcggct
    49681 ggaccgtaaa atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt
    49741 tttgatgttg ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc
    49801 cgcgatgatg ctgcctctgg caatgggtat gctgagccac ctcgaccagg aaaaagaaca
    49861 caaaacctac gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg
    49921 cacgctcgtc ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt
    49981 cggctggatg aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc
    50041 cctgtacgtc atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat
    50101 cccttggacg ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg
    50161 gatattcagc tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc
    50221 cctgagtgcc gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg
    50281 caataccgac tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt
    50341 gaaaacatcc ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc
    50401 gcccgcattt ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac
    50461 cagcaacacc gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat
    50521 ggggctgccc gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat
    50581 gctgccggtt gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg
    50641 cgaaatgatg aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg
    50701 ggcttatgct gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat
    50761 ttttcagacg accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccag
    50821 attctaacga aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg
    50881 aactcgattt cgacaaagcc cctgttaccg ccaaaaactt cgagcaatac gtcaaagacg
    50941 gcttctacga cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg
    51001 gcatggatga aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca
    51061 acggcctgcc caacgataaa tacaccatcg ccatggcacg cacttccgac ccccattccg
    51121 ccagcgcgca attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag
    51181 agctgtacgg caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg
    51241 acggttttga cgttgtcgat gccatcgaag gcgtttctac caaacgtcat ggttaccacg
    51301 acgacgtacc gagcgaacct gtcgtcatca ttaaagccga agcggtataa accgacaatc
    51361 cgaaagcagc ctgcacaaag gctgctttta tttcagacgg catgttattt gatgtaaccg
    51421 tgctatgccg tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata
    51481 tgcccgtttt tcctaaacgg acacaaagga aaccttatgg caaatattgc ccgcgacatc
    51541 ttcaaagcct acgacatccg aggcattgtc ggcaaaaccc tgaccgacga tgccgcctat
    51601 ctcatcggca gggccatcgc cgccaaagcc gccgaaaaag gcatgacccg catcgcgctc
    51661 ggacgcgacg gacgcttgag cggtcccgaa ctgatggagc acatccaacg cggctttacc
    51721 gacagcggca tcgacgtcct caacgtcggt atggtcgcca cgcccatgct ctattttgcc
    51781 gccatcaacg aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcccgat
    51841 tacaatggtt tcaaaatgat gctcggcggc gacacgctcg caggcgaagc cattcaagaa
    51901 cttttagcca ttattgaaaa agacggtttt gttgccgccg acaaacaagg caacgtaacc
    51961 gaaaaagaca tctccggcga ataccacaac cacatcgtcg gccacatcaa actcaaacgc
    52021 cccatgaaaa tcgccatcga cgcaggcaac ggcgtaggcg gcgcattcgc aggcaaactc
    52081 tacaaaggct tgggcaatga agttaccgaa cttttctgcg aagtggacgg caacttccct
    52141 aatcaccacc ccgacccttc caaaccgaaa aacctgcaag acctcatcgt cgagctcaaa
    52201 aacggcgagg ccgaaatcgg cttagcattt gacggcgatg ccgaccgctt gggcgtggtt
    52261 accaaagacg gcaacatcat ctatccggac cgccaactga tgcttttcgc gcaagacgtg
    52321 ttgagccgca atcctaaagc caaagtgatt ttcgacgtca aatccacccg tctgcttgcg
    52381 ccttggatta aagaacacgg cggcgaacct gtgatggaaa aaaccggcca cagctttatc
    52441 aaatccgcca tgaaaaaaac cggcgcgccg gttgccggcg aaatgagcgg acacatcttc
    52501 ttcaaagaac gctggttcgg cttcgacgac ggtctgtacg ccggcgcacg cctcttggaa
    52561 atcctgtctg cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc
    52621 acgcccgaac tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa
    52681 ctcgccgcca aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtctgcgc
    52741 gttgaatttc ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattctggtg
    52801 ttgcgttttg aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc
    52861 gtcatcgaaa gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc
    52921 aaatcttggt agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt
    52981 cgaacaactc tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg
    53041 tcgtggcaac ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt
    53101 cccgcgccaa ttccttcatg gcgttcaaca cgtcttgcac caactcgggg tcaagcgcgg
    53161 aagtgggttc gtcaaacaac atcagctcgg gctgaatcgc caatgctcgg gcaatgccga
    53221 cgcgctgctg ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc
    53281 cgactttttc cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta
    53341 cggcaaccgg tccttccatc acgttttcca aggcggtttt gtgcggaaag aggttgtatt
    53401 gttgaaacac catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt
    53461 tagaaaaatc gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca
    53521 tttccaacgc gtttaggcat cgcagaaacg tcgttttacc cgagccggaa ggcccgagga
    53581 tgacgaccac ctgccctttg cacacatcca aattgatgcc gcgcaaaatg gtatttttgc
    53641 cgaaggtctt atggatattg cggattttaa tcatgacaac tccttatttg gcgacatagc
    53701 ggtcgaagcg tttttccaag cgcgcctgaa tcaggaacag cactttgcaa aaacaccagt
    53761 aaaccaaagc ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct
    53821 gcgcgacgcg gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca
    53881 aaccgataaa ctcgttgctc aaaggcggca cggcaacgcg aaatgcctgc ggcgcgacga
    53941 tgcggcggaa cgtctgcata taggtcatgc cgatggagaa acctgcttcc cattggcctt
    54001 tcggtacgga caaaattgcc gcgcgtatgg tttcggaagc atatgcgccg acattgagcg
    54061 aaaagccgat gatggcggca ggaatcgggt cgatatagat gccgacggaa ggcagcccgt
    54121 aaaacacaat cacaagctga accaacagcg gcgtaccgcg aatgacggaa atataaaatt
    54181 ccaccaattt cagcaggatt ttccgcacga tgccgccggc gggcataatc cgcaccaaag
    54241 ccacggctac cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa ggcagagaga
    54301 ccgcgaagcc ggctttgacc ataggcaaaa acgcgctgac aatcatatcg gcgcgtgttt
    54361 ccgtcataaa cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt tccgaagaat
    54421 tgttcgccca gttttttcag cgtaccgtcg gctttcagct cgttgattgc cgtactgaat
    54481 ttcgccacgg cttcgtcatt gcccttgttg acaatcaggc tggaaccgac tttttcatcg
    54541 gcaggtgcgg accaaacgat tttcacgccc gcattcgggt ttttcttcag atagtccaaa
    54601 accgccaatt cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag ggtcagcgat
    54661 tgcgccaaac cgtcaacagc caccaaatct gcgcctgcag ctttggcttt ttcgccgtag
    54721 ttgctggtca gggattgtgc ggttttcacg cctttgatgt cgtcgataga tttgatgttg
    54781 ctgtcgttac gggcaaccaa tacggcaccg ctccagctgt aaggatcgga tttgtcgaat
    54841 gtcgcttggc gttcggggct ggtcagaccg acttggtttg ccaccacgtc gaaacgcccc
    54901 gccttcaaac ccgccatcat cgaatcccat tgcgtttctt taaactcgac tttcacgccc
    54961 agtttttccg ccacggcgcg ggttacttcc acatcgtaac cggtcagttt gccgtccttg
    55021 tcgtggtagg taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt gcctttattg
    55081 ttgatgcgct cgattaaaga accggaaact gccgattgtg caggcgcgga agatgctccg
    55141 ctgccgcttt ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc gccgagtacg
    55201 aattttttca acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt ctaagtgaaa
    55261 ttgcggcaaa cacaaagaat acttttttta tatggttatt ccaaatcgga atggaaaaga
    55321 tatgccgaag ccgccggaag cggaaataaa gggggcggga aaggcgacgg gcggcacgaa
    55381 accgaaaagc ggcatcaggt atgcaaacgg cggattcacg cacgccgttc cggcaaaaat
    55441 ccgcctgccg attcggtgct ataatccatc cgaaacagat gacaccaaaa aggaacatct
    55501 catgaacacg ccccaatccg ccatcattcc cgaccacgcc caagccggca tctttatcga
    55561 agccgacttc gccgccaacc gcctcaacga tattaaagcc gcctgccgcg cttcgctcga
    55621 cgcgttatcc gccttgaaaa cccgctttcc ggacgatatt ttgggtctga ccatcgcctt
    55681 cggcagcaaa gcctgggcaa cattcggaca tacggacgaa ggcagtgaaa tcaaaccctt
    55741 ctccgaaatg ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca tccacatcca
    55801 atctttccgc caaaacgccg cctacgcgct tgcccaatcc gttttgggcg cgttcggcga
    55861 cagcatatgc gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc gcgggctgga
    55921 cggtttcgtc gacggcacgg aaaacccgca gggcgatgaa aatgtccgcg aagtcgccat
    55981 catccccgaa ggaaaacccg atgcgggcgg cagctatgtc ctgctgcaaa aatacctgca
    56041 cgatttgaaa aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg tcggacgcgg
    56101 caaggaaacc gacgacgaat tcggccacga tgtccgcctg cccgattcgc acctcggccg
    56161 cgtcaacctg aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc tgcccttcgg
    56221 caaaatcagc ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc tgcacaatat
    56281 cgaagcgcaa ttattgagta tgttcggcga cacggatggc aaaaccgacc tgctgctccg
    56341 gcacctctcc gccgccgttt cgggcggata ctactacgcg ccgtctgtcg aacgcctgca
    56401 aaacctctaa gccagacgat gccgtctgaa accatttttc agacggcatt tt
//
LOCUS       DAWKQJ010000002        42408 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000002 DAWKQJ010000000
VERSION     DAWKQJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.002"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            329..1294
                     /locus_tag="WJL20_000056"
     CDS             329..1294
                     /locus_tag="WJL20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7682278.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNLLPYLDEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1458..2426
                     /locus_tag="WJL20_000057"
     CDS             1458..2426
                     /locus_tag="WJL20_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7682279.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFVAY
                     EFEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADQLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVVVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
     gene            2416..3390
                     /locus_tag="WJL20_000058"
     CDS             2416..3390
                     /locus_tag="WJL20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ7682280.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3435..4061
                     /locus_tag="WJL20_000059"
     CDS             3435..4061
                     /locus_tag="WJL20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682281.1"
                     /translation="MTQERFPSFFNQAPTITVRDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGGRDEGTTGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASAEELERFRQLWQARVKAFLTESADDPQFVIVREM"
     gene            4146..4904
                     /locus_tag="WJL20_000060"
     CDS             4146..4904
                     /locus_tag="WJL20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7682282.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPSAECRRIVNGAIEEFHLKDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGQVALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4963..5301)
                     /locus_tag="WJL20_000061"
     CDS             complement(4963..5301)
                     /locus_tag="WJL20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ7682283.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5452..9414
                     /gene="purL"
                     /locus_tag="WJL20_000062"
     CDS             5452..9414
                     /gene="purL"
                     /locus_tag="WJL20_000062"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ7682284.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRSDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9509..10267
                     /gene="gloB"
                     /locus_tag="WJL20_000063"
     CDS             9509..10267
                     /gene="gloB"
                     /locus_tag="WJL20_000063"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ7682285.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10574..15046
                     /gene="mspA"
                     /locus_tag="WJL20_000064"
                     /gene_synonym="ausI"
     CDS             10574..15046
                     /gene="mspA"
                     /locus_tag="WJL20_000064"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ7682286.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFTVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGNSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNNTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNSPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDITGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKNTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPLKLEGDSRGAFQIH
                     VKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPL
                     KEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARID
                     SQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELDT
                     YIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLA
                     ELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRP
                     YQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGA
                     LFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNG
                     NRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSV
                     NDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            15278..16732
                     /gene="mgtE"
                     /locus_tag="WJL20_000065"
     CDS             15278..16732
                     /gene="mgtE"
                     /locus_tag="WJL20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ7682287.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDASLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDNLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16818..17726)
                     /gene="hslO"
                     /locus_tag="WJL20_000066"
     CDS             complement(16818..17726)
                     /gene="hslO"
                     /locus_tag="WJL20_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ7682288.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            17971..18726
                     /locus_tag="WJL20_000067"
     CDS             17971..18726
                     /locus_tag="WJL20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7682289.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18745..18963
                     /locus_tag="WJL20_000068"
     CDS             18745..18963
                     /locus_tag="WJL20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682290.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19108..19452
                     /locus_tag="WJL20_000069"
     CDS             19108..19452
                     /locus_tag="WJL20_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ7682291.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19452..20144
                     /locus_tag="WJL20_000070"
     CDS             19452..20144
                     /locus_tag="WJL20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ7682292.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20211..21431
                     /gene="argJ"
                     /locus_tag="WJL20_000071"
     CDS             20211..21431
                     /gene="argJ"
                     /locus_tag="WJL20_000071"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ7682293.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21491..22900
                     /locus_tag="WJL20_000072"
     CDS             21491..22900
                     /locus_tag="WJL20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7682294.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23090..24481
                     /gene="hrpA"
                     /locus_tag="WJL20_000073"
     CDS             23090..24481
                     /gene="hrpA"
                     /locus_tag="WJL20_000073"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ7682295.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24582..25709
                     /locus_tag="WJL20_000074"
     CDS             24582..25709
                     /locus_tag="WJL20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682296.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            25886..27463
                     /gene="lpt3"
                     /locus_tag="WJL20_000075"
     CDS             25886..27463
                     /gene="lpt3"
                     /locus_tag="WJL20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ7682297.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            27565..30840
                     /locus_tag="WJL20_000076"
     CDS             27565..30840
                     /locus_tag="WJL20_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ7682298.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFSATTANPLPNPLP
                     QEGEQSAAASTISDDLHPANPQQTALSPVGEGWGEGKTVASQTNFSATTANPLPNPLP
                     QEGEQSAAASAVSNDPKPKKQPAPPKGRLKPLPLADIRTFQAWIKTAERDNLRLLFLS
                     RDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPA
                     ALEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAI
                     AKTAGDIRILEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAA
                     AVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDT
                     TEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVC
                     DRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPL
                     THLLRLRLQTLLAPGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQE
                     LEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWE
                     KIF"
     gene            30886..31737
                     /locus_tag="WJL20_000077"
     CDS             30886..31737
                     /locus_tag="WJL20_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7682299.1"
                     /translation="MNTISSIMSLENFQLIKTQLGVPSYENDRVAIYQGDCIQLMDKI
                     SKGSFQMTITSPPYNIGKEYESILDLEHYLEWCERWMQSIHHLTEDSGCFWLNLGYLH
                     VEKKGLAVPIPYLLWDKTNFFLLQEVVWNYAAGVACRNRFSPRNEKLLWYVKNPAKYT
                     FNLDAVRDPNVKYPNQKKNGKLKCNPLGKNPTDVWQIAKVTSGRNRSSRERTAHPAQF
                     PLELIERIIRASSNQNDVILDPFIGSGTTALAGLKNNRFVIGFEINDEYIDIIKERFK
                     EYDLQNT"
     gene            complement(31794..32393)
                     /locus_tag="WJL20_000078"
     CDS             complement(31794..32393)
                     /locus_tag="WJL20_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScaI family restriction endonuclease"
                     /protein_id="HEZ7682300.1"
                     /translation="MNRSKISPYASLNEELWISKTEELIKQHPLSEKEIVQLCLDSWE
                     NIFSSRIGNLQIGKNFFPSPQIVGALLHALIPANIEARFENWQGEKNKYDKDIVYIKD
                     DYYSIELKTSSDPKYIFGNRSYAQPQDGITGKSKNGYYLTINFEKFMPDLKLRPEIKI
                     IRFGWLDHTDWIAQTAATGQQARLSPEAYRSKLKILYQK"
     gene            complement(32395..32610)
                     /locus_tag="WJL20_000079"
     CDS             complement(32395..32610)
                     /locus_tag="WJL20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7682301.1"
                     /translation="MENELVEFGKRVKHYRLLNGWSQEELAEKVGLHRTYIGSVERGE
                     RNISLLNISLLAKSFSINISELVEGIK"
     gene            complement(33042..33509)
                     /locus_tag="WJL20_000080"
                     /pseudo
     CDS             complement(33042..33509)
                     /locus_tag="WJL20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            33741..33953
                     /locus_tag="WJL20_000081"
     CDS             33741..33953
                     /locus_tag="WJL20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682302.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            34250..34405
                     /locus_tag="WJL20_000082"
     CDS             34250..34405
                     /locus_tag="WJL20_000082"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ7682303.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(34495..34944)
                     /locus_tag="WJL20_000083"
     CDS             complement(34495..34944)
                     /locus_tag="WJL20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7682304.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(35032..35496)
                     /locus_tag="WJL20_000084"
     CDS             complement(35032..35496)
                     /locus_tag="WJL20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ7682305.1"
                     /translation="MLYPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPAPKGPAKPVLPADKK"
     gene            complement(35532..35645)
                     /gene="rrf"
                     /locus_tag="WJL20_000085"
     rRNA            complement(35532..35645)
                     /gene="rrf"
                     /locus_tag="WJL20_000085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(35742..38633)
                     /locus_tag="WJL20_000086"
     rRNA            complement(35742..38633)
                     /locus_tag="WJL20_000086"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(39039..39114)
                     /locus_tag="WJL20_000087"
     tRNA            complement(39039..39114)
                     /locus_tag="WJL20_000087"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39079..39081),aa:Ala,seq:tgc)
     gene            complement(39120..39196)
                     /locus_tag="WJL20_000088"
     tRNA            complement(39120..39196)
                     /locus_tag="WJL20_000088"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39160..39162),aa:Ile,seq:gat)
     gene            complement(39298..40838)
                     /locus_tag="WJL20_000089"
     rRNA            complement(39298..40838)
                     /locus_tag="WJL20_000089"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(41178..41834)
                     /locus_tag="WJL20_000090"
     CDS             complement(41178..41834)
                     /locus_tag="WJL20_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ7682306.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
     gene            complement(41866..42378)
                     /gene="coaD"
                     /locus_tag="WJL20_000091"
     CDS             complement(41866..42378)
                     /gene="coaD"
                     /locus_tag="WJL20_000091"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ7682307.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
BASE COUNT        10436 a        12446 c        10000 g         9526 t
ORIGIN      
        1 gttgcgggaa tgacgaaaag tggtgggaat gacggttcgg gcattcctta aattacccgt
       61 gtatcgctgt aaatcttaga gatggcggaa tatagcggat gaacaaaaac cagtacgtcg
      121 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaaccg
      181 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
      241 ctatagatta tcatttatcc tttctaaagc cgttccggtt tgtccgactg gcggctttgc
      301 cccaatatcc ccattttgga gacaccctat gttgcgtttg accgcattga ccctctgcac
      361 cgtcctcgcc ttgggcgcgt gttcgccgca aaattccgac tctgccccac aagccaaaga
      421 acaggcagtt tccgccgcac aatccgaaag cgcgtccgtt accgtcaaaa ccgcgcgcgg
      481 cgacgttcaa ataccgcaaa accccgaacg catcgccgtt tacgatttgg gtatgctcga
      541 caccttgagc aaactgggcg tgaaaaccgg tttgtccgtc gataaaaacc tgctgcctta
      601 tttggacgaa tatttcaaaa ccacgaaacc tgccggtacg ctgtttgagc cggattacga
      661 agcactcaac gcttacaaac cgcagctcat catcatcggc agccgcgcag ccaaagcgtt
      721 tgataagttg aacgccattg cgccgaccat cgagatgacc gccgataccg ccaacctcaa
      781 agaaagtgcc aaagagcgta tcgacgcgct ggcgcaaatc ttcggcaaac aggccgaagc
      841 ggacaagctg aaagcggaaa tcgacgcgtc ttttgaagcc gcgaaaactg ccgcacaagg
      901 caaaggcaaa ggtttggtga ttttggtcaa cggcggcaag atgtccgcct tcggcccgtc
      961 ttcacgactg ggcggctggc tgcacaaaga catcggcgtt cccgctgttg acgaagccat
     1021 caaagaaggc agccacggtc agcctatcag ctttgaatac ctgaaagaga aaaatcccga
     1081 ctggctgttt gtccttgacc gcagcgcggc catcggcgaa gagggtcagg cggcgaaaga
     1141 cgtgttgaac aatccgctgg ttgccgaaac aaccgcttgg aaaaaaggac aagtcgttta
     1201 ccttgttcct gaaacttatt tggcagccgg tggcgcgcaa gagctactga atgcaagcaa
     1261 acaggttgcc gacgctttta acgcggcaaa ataatgaaac ggcggcattc gatgccgtct
     1321 gaaacacgga tgcaaaccgc ttcctgtgtt tcagacggca ttgcccgata cggaggcttc
     1381 aaacaaggct ttccgctccg acggttcgga ctgccttgtt tgaatcttct acgccttaac
     1441 gcttttccct tctgtttatg actgccaaac ctttttccct caacctgacc aacctcctgc
     1501 tgctggcggt gttgtttgcc gtcagcctgt cggtgggcgt tgccgatttc cgctggtctg
     1561 atgtgttttc gctgtcggac agccagcagg ttatgttcat cagccgcctg ccgcgcacgt
     1621 ttgcgattgt gctgacgggc gcgtcgatgg cggtggcggg gatgattatg cagattctga
     1681 tgcgtaaccg ttttgtcgag ccttctatgg cgggcgcggg tcagagtgcg gctttgggtt
     1741 tgcttctgat gtccctgctg ctgcctgccg cgccgctgcc ggtcaaaatg tcggttgccg
     1801 ccgttgccgc gctaatcgga atgctggtgt ttatgctgct gatacgccgc ctgccgccga
     1861 cggcgcaatt aatggtgccg ctggtgggga ttattttcgg cggcgtggtt gaggcggtgg
     1921 cgacgtttgt cgcgtatgag tttgagatgc tgcaaatgtt gggcgtatgg caacagggcg
     1981 atttttccgg cgtgttgctc gggcggtatg agctgttgtg ggcaacgggg attttggctt
     2041 tgtttgccta tttgattgcc gaccagctga cgattttggg tttgggcgaa acggtgagtg
     2101 tgaacttggg gctgaaccgg acggcaattc tgtggtcggg gttgattatt gtggcgctga
     2161 tcacgtcttt ggtggtcgtg acggtcggca atattccgtt tatcggcttg gtcgtgccga
     2221 acatcatcag ccgcctgatg ggagacaagc tgcgccaaag cctgcctgct gtggctttac
     2281 tgggcgcatc tttggtgttg ctgtgcgata ttgtcggacg cgtgattgtg tttccgtttg
     2341 aaattccggt ctctacggtt tttggcgtga tggggacggc tttgttttta tggcttttat
     2401 taaggaaacc tgctcatgcc gtctgaaaaa aatatcggtt ttatggcagg aagcagccgt
     2461 ccgttgtggg tcgcttttgc actgttgctg gtttcctgcg ttctgtttat gacgctcaac
     2521 gtcaaaggcg attgggactt tgttttgcac ctgcgcctga ccaagcttgc cgcgctactg
     2581 atggtcgcct atgcggtcgg cgtttccacc cagctttttc aaacgctgac caacaatccg
     2641 attctgaccc cttcgatttt gggtttcgat tcgctgtatg tgtttttgca gaccttgctg
     2701 gtgtttacgt tcggcggcgt gggctatgct tccctgccgt tgacgggcaa attcggcttt
     2761 gaactggtcg ttatgatggg cggctcgctg ctgctgtttt acacgctcat caaacagggc
     2821 gggcgcgatt tgccgcgtat gattttaatc ggcgtgattt tcgggatttt gttccgcagc
     2881 ctgtcgtcgc tgctttcgcg catgatcgac cccgaagaat ttacggcggc gcaggcgaat
     2941 atgtttgccg gattcaatac cgtccacagc gagcttttgg gcataggcgc gctgattctg
     3001 ctcgtcagcg cggcggtcgt ttggcgtgaa cgctaccgct tggacgtaca ccttttgggg
     3061 cgcgaccaag ccgtcaattt gggcatcagc tacacgcgca acaccttatg gatactgctt
     3121 tggattgccg cgctggtggc gacggcgacc gccgttgtcg gtccggtaag ctttttcggg
     3181 cttctcgccg cctcgcttgc caaccacttt tccccgtccg tgcgccattc cgtccgccta
     3241 ccgatgacgg tttgtgtcgg cggcatcctc ttggtcggcg gacaaaccgt attcgaacac
     3301 ttcttgggca tgaaggcggt attaagcgtg gtggtcgaat ttgcgggcgg actcgttttc
     3361 ctctatctcg ttttaaaaca caaaaaatga cggatgccgt ctgaacggca gcccgactcg
     3421 aaaggacaaa ccatatgacg caagaacgtt tcccatcatt cttcaaccaa gccccgacca
     3481 tcaccgtcag agacccgctt gccgcattcc tcggcgcggc cgaaaacggc atcctcactt
     3541 accgctacgc cgatgccgtg cgcctgtgcg gacattcctg cccgaccgtc gcgggcgcgt
     3601 acctgatggt tatcaaaggt ctgaaagccc tttacggcga agagctgccc gaacgcggcg
     3661 gcatcgaagc ctttatgcag ggcgggcgag acgaaggcac gaccggtgtt accgcttccg
     3721 tcgtccaact cctcaccggc gcagccccgg aaaccggctt cggaggcatc ggaatgcagg
     3781 gacgcttcgc ccgccgcaac ctcttgtcct ttggtgcagg cgaaatcaac ggcacactcg
     3841 cactccgccg ccgcgatacc ggcaaaaccg ttgccgtcag cctcaacgcc gcgctccaac
     3901 cttttgcacc gcaaatgcgc gacatcatgc ccaaagccgt cagcggcagc gcaagcgcag
     3961 aagaactcga acgcttcaga caactctggc aggcgcgcgt taaagcattt ttgaccgaat
     4021 cggcggacga cccgcagttc gtcatcgtcc gcgaaatgtg agcgttcaga cggcattccg
     4081 aatttcaaat accgtccgaa cccccgctaa acatacaaca aacctacgcc cgacaagcat
     4141 ccgccatgat taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca
     4201 acgtcagcct cgacatcccc gaaggcggca ttaccgcgct cgtcggcccc aacggcgcgg
     4261 gcaaatccac cctgttttcc tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg
     4321 cctaccgcgg caaaaatctt gccggcaccc ccaccgccga actcgccaaa actctgtcca
     4381 tcctcaccca agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctgatgttcg
     4441 gccgttaccc ctaccatcaa ggcagaccgt ctgccgaatg ccgccgtatc gttaacggtg
     4501 caatcgaaga attccacctg aaagacctct ccgaccgcta tctgaccgag ctctccggcg
     4561 gacaacgcca acgcgccatg attgcgatgg tgttctgcca aagcaccgac tacgtccttt
     4621 tggacgaacc gctgaacaac ctcgatatgt atcacgcccg ctcgctcatg cagattctgc
     4681 aaaagctcac gcgcgaacac aaacgcacca ccgtcgccgt attgcacgac attaaccagg
     4741 ccgccgctta cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc ctgaccggca
     4801 cgcccgaaga agtgttcacc gtacacaaca tcaaagaact gttcgacatg gacgtggacg
     4861 tactcgacta cgaaggcaaa aaactcatcg tccaccatat ctgaagctca aaaagaaaat
     4921 gccgtctgaa aggctttcag acggcatttc cattccaaac gctcaaaccg ccgcgtccgc
     4981 acgttcgccc gtgcggatac ggattgcctc ctcgaccggc agcacaaaaa tcttgccgtc
     5041 gccgattttg cccgaacgcg ccacctcgac aatcacgtca atcgcgcgtt ccacagcgtc
     5101 atccgccaac accagctcga ttttgacctt gggcaggaaa tcgacagcgt attccgcgcc
     5161 ccggtaaatc tccgtatgcc ccttctgcct gccgaaccct ttgacctcgc tgacggtcat
     5221 acccgtaatg ccgatttccg tcaacgcctc gcgcacgtcg tcgagtttga acggtttgac
     5281 aatcgcctcg atttttttca taaaatttcc tttgaacaaa caatacaaac acatccgaaa
     5341 aacgggaacc tcccgtcaga ttgtcaacat tttaagccaa aatacccaag caatacagcc
     5401 ccgttgcgcg tataatgaca gattttccaa ccgcatttga gagccgaatc catgtctgtc
     5461 gttttgccct tgcgcggcgt taccgccctt tccgatttcc gtgtcgaaaa actcttgcaa
     5521 aaagccgccg cactcggact gcccgaagtc aaattaagca gcgaattttg gtatttcgcc
     5581 ggcagcgaga aagcattgga tgccgcgact gtcgaaaaac tgcaagcctt gttggcggcg
     5641 caaagcgttg aacaaacgcc aaaagcgcgc gagggcttgc atttgttttt ggtcacgccc
     5701 cgtttgggta cgatttcgcc gtgggcttcc aaggcgacca atatcgcgga aaactgcggt
     5761 ttggcaggca tcgagcgcat cgagcgcggc atggcggtgt ggctggaagg tgcgcttacc
     5821 gatgaacaga aacggcaatg ggcggcgttg ctgcacgacc gcatgaccga atcggtgctg
     5881 cccgattttc agacggcatc caaattattc caccatctcg aatccgaaac cttttccacc
     5941 gttgatgttt taggcggcgg taaagaggct ttggtcaaag ccaataccga aatgggtttg
     6001 gcactctccg ccgacgaaat cgattatctg gttgaaaact atcaggcttt gcagcgcaat
     6061 ccgtccgatg ttgaattgat gatgttcgcg caggcaaaca gcgaacactg ccgccacaaa
     6121 atcttcaacg ccgatttcat cctcaacggc gaaaaacagc cgaaatccct gttcggtatg
     6181 atacgcgaca cgcacaacgc gcatcccgaa ggcacggtcg tcgcttataa agacaattcg
     6241 tccgtaattg aaggcgcgaa aatcgagcgt ttctatccga atgcggcgga aaaccaaggt
     6301 taccgtttcc acgaagaaga cacgcatatc atcatgaaag tggaaacgca caaccacccg
     6361 accgccatcg cgccgtttgc gggcgcggca acaggcgcgg gcggcgaaat ccgcgacgaa
     6421 ggcgcgacgg gcaaaggttc gcgtccgaaa gcaggcttga ccggctttac cgtctccaac
     6481 ctcaacatcc ccgacctcaa acagccgtgg gaacaagact acggcaagcc ggaacacatt
     6541 tcctcgccgt tggacattat gattgaaggc cccatcggcg gcgcggcgtt caacaacgaa
     6601 ttcggccgcc ccaacctctt gggctacttc cgcacttttg aagagaagtt tgacggtcag
     6661 gttcgcggct atcacaaacc gattatgatt gccggcggct tgggcagcat tcaggcgcag
     6721 cagacgcata aagacgaaat ccccgaaggc gcattgctga ttcaactggg cggcccgggc
     6781 atgcttatcg gcttgggcgg cggcgcggct tcttcgatgg ataccggcac gaacgacgcg
     6841 tctttagact tcaattccgt acaacgcggc aaccccgaaa tcgaacgccg cgcgcaggaa
     6901 gtcatcgacc gctgctggca gctcggcggc aaaaacccga ttatctccat ccacgacgtg
     6961 ggcgcgggcg gtttgtccaa cgccttcccc gaactcgtca acgatgccgg acgcggcgcg
     7021 gtattcaagc tgcgcgaagt gccgttggaa gaacacggtt taacgccttt gcaaatctgg
     7081 tgtaacgaat cgcaagagcg ttatgtgttg tcgattttgg aaaaagattt ggacaccttc
     7141 cgcgccatct gcgaacgcga acgctgcccg tttgccgtgg tcggcacggc gaccgacgac
     7201 ggccatttga aagtgcgcga cgatttgttc gccaacaacc ccgtcgattt gccgctgaac
     7261 gtcttgctcg gcaaaccgcc caaaaccacg cgcagcgaca aaacggttac gccatctaaa
     7321 aaaccgtttc acgccgacga tatcgacatc accgaagccg cctaccgcgt tttgcgcctg
     7381 cctgccgtag ccgccaaaaa cttcctgatt accatcggcg accgcagcgt cggcggcatg
     7441 acccaccgcg accaaatggt cggcaaatac caaacccccg tagccgactg cgccgttacc
     7501 atgatgggct tcaataccta tcgcggcgaa gcaatgtcta tgggcgaaaa accgaccgtc
     7561 gccctgtttg acgcgcctgc ttcgggcaga atgtgcgtcg gcgaagccat caccaacatc
     7621 gcggcggtca acatcggcga catcggcaac atcaagctct ccgccaactg gatggcggcg
     7681 tgcggcaacg aaggcgaaga cgaaaaactc taccgcaccg tcgaagcggt ttccaaagcc
     7741 tgtcaggcat tggatttgag cattcccgtg ggcaaagaca gcctgtcgat gaaaaccgta
     7801 tggcaggatg gcgaagagaa aaaatccgtc gtttcgccgt tgagcctgat tatctccgcg
     7861 ttcgcgccgg ttcaagacgt acgcaaaacc gttacgcccg aattgaaaaa cgtcgaggac
     7921 agcgtattgc tgtttgtcga tttgggcttc ggcaaagcgc gtatgggcgg ctctgcattc
     7981 ggtcaggtat acaacaacat gagcggcgac gcgcccgatt tggacgacac aggccgtctg
     8041 aaagcgtttt acaacgtgat tcaacagctt gtcgccgaag acaaactctt ggcataccac
     8101 gaccgcagcg acggcggctt gtttgccgtt ttggtagaaa tggcgtttgc agggcggtgc
     8161 ggcttggata tagatttaaa tttattgctt gcacaaacat ttattaccaa ccataccgct
     8221 ctgtctcaat cattgcggac tgaagaggta aaagcgttgg ctgaatggca agaaaccatt
     8281 gcccgcacat tatttaatga agagttgggt gctgttatcc aagttagaaa acaagatgtt
     8341 gccgatatta tcaatttatt ctatcaacaa cagctgcatc ataatgtctt tgaaatcggt
     8401 acgttaactg atgagaacac gttaatcatc cgcgacgggc aaacgcacct tatttctgac
     8461 aacctaatca aactgcaaca aacctggcaa gaaaccagcc atcaaatcca acgcctgcgc
     8521 gacaaccctg cctgcgccga cagcgagttt gccctgattg gcgacaacga ccgcagcgca
     8581 ttgtttgcca acctgaaatt cgacgtgaac gaagacatcg ccgcgccgtt tatcaacagc
     8641 ggcgcgaaac ccaaaatcgc catcctgcgc gaacagggcg tgaacgggca aatcgaaatg
     8701 gccgccgcct tcacccgcgc cggattcgat gcttacgacg tgcatatgtc cgacctgatg
     8761 gcaggccgct tccgccttgc cgacttcaaa atgctggcgg cgtgcggcgg cttcagctac
     8821 ggcgacgtac tcggcgcggg cgaaggctgg gcgaaatcca tcctgttcca ccctgccttg
     8881 cgcgaccaat ttgccgcctt ctttgccgat ccggacacgc tgacattggg cgtgtgcaac
     8941 ggctgccaga tggtcagcaa cctcgccgag attatcccgg gtgcagaggc atggcctaaa
     9001 ttcaaacgca acctgagcga acagtttgaa gcacgcctga gcatggttca cgttccgaaa
     9061 tcagcgtcgc tgattctgaa cgaaatgcaa ggctccagcc tgcctgtcgt ggtcagccac
     9121 ggcgaaggcc gcgccgactt cgcgcttcac ggcggcaata tttccgccga tttgggcatc
     9181 gcgctgcaat atgtggacgg tcaaaaccaa gtgacccaaa cctacccgct caaccccaac
     9241 ggctcgccgc aaggcatcgc cggcgttacc aacgccgacg gccgcgtgac catcatgatg
     9301 ccgcatccgg aacgcgtgta ccgtgccgca caaatgagct ggaaaccaga agactggacg
     9361 gaattgtccg gctggtaccg cctctttgcc ggcgcacgta aagccttggg ctaaccgccc
     9421 tactcaaacc aatgccgtct gaagaatatt tcagacggcg ttccggcata ccatccttta
     9481 aacggtatcc gtccaccgag gaacactcat gaaaatcacc cccgtcaaag ccctaaccga
     9541 caactacatc tggatgatac agcacggcaa ccatgccgtc tgcgtcgacc cttccgaacc
     9601 ctcgcccgtc ttggaattcc tcgtccgcaa ccgcctcatg cttgcccaaa catgggtaac
     9661 tcacccccac cccgaccacg agggcggtgc ggcggcactc tggcgcggct acatggaatc
     9721 gcccgtttac ggcgaatccg acatcgaagc agcaacccac accgtaaccg ccggcaccca
     9781 attcaccttc ggcgacggac aggttaccgt ttgggcaaca cccggccaca cagaccgcca
     9841 caccagctac cttctcgaaa cttcagacgg catacacgtc ttttgcggcg acaccctttt
     9901 ttccgccggc tgcggacgcg tgtttaccgg cacaatcgaa cagctttacg acagcttcca
     9961 acgcttcaac cgcctgcctg aaaacaccct gttctatccg gcgcacgaat acaccgccgc
    10021 caacctgcgt ttcgccgccc atatcgagcc ggacaacgcc gacattcaga cggcactgaa
    10081 agcggcggcg catacgccta ccctgcccgt taccctcgcg cacgaacgcc gtgtcaatcc
    10141 gtttttgcgc gtcgacctgc cgcacgtcag agaccgcgcc gaggcattga gcgggaaaac
    10201 gttaaacagc agcctcgata cctttgtcgc gctgcgtgaa cttaaaaacc aataccggac
    10261 gaaataaaac aacgggaaaa cgcagccatt cctaggattt ttattaaaat cttaaataaa
    10321 atcatacaat catcgccaat agacgaaagg acaccgttgc cttataatca aacaaaaaca
    10381 aaatatataa tatagtggat tgaatttaaa tcaggacaag gcgacgaagc cgcagacaat
    10441 acagatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc actatataaa
    10501 tccagcacaa aacgggatcg gtgattcttg tccgcaagaa tcgttgattt tctctattac
    10561 acggataatc atcatgcgct tcacacacac caccccattt tgttccgtat tgtccaccct
    10621 cggtcttttt gccgtttccc ctgcttactc atccattgtc cgcaacgatg tcgattacca
    10681 atattttcgc gactttgccg aaaataaagg cgcgttcacc gtaggtgcaa gcaatatttc
    10741 catccaagac aagcaaggca aaatattagg cagggttctc aacggcatcc ccatgcccga
    10801 cttccgcgtc agcaaccgcc aaaccgccat cgccaccctg gttcaccccc aatacgtcaa
    10861 cagtgtcaaa cacaacgtcg gctacggttc catacaattc ggcaacgaca cccaaaatcc
    10921 agaagaacaa gcctatacct accgcctcgt atcacgcaac ccgcacccgg actacgacta
    10981 ccaccttccc cgcctcaaca aactggttac cgaaatctca cctgccgcac tcagcagcgt
    11041 acccttgctt ggaaacggcc agccaaaggc caatgcctac ctcgataccg accgcttccc
    11101 ctactttgta cgactcggct caggcacgca acaagtccgc aaagcagacg gcacgcgtac
    11161 acgaaccgcc ccagcatacc aatacctgac cggcggcacg ccgctgaaag tattggggtt
    11221 ccaaaaccac ggcttactcg tcggcggcag cctgaccgac caacccctta acacctacgc
    11281 aatcgccgga gacagcggtt cccccctgtt tgccttcgac aagcatgaaa accgctgggt
    11341 gcttgcgggc gtactcagca cctacgccgg cttcgacaac ttcttcaaca aatacatcgt
    11401 cacgcaaccc gaattcatcc gttccaccat ccgccaatac gaaacccggc tggatgtcgg
    11461 gctgaccacc aacgaactca tatggcgcga caacggtaat ggcaacagca ccctgcaagg
    11521 gctcaacgaa cgcatcaccc tgcccattgc aaacccttcg cttgccccac aaaacgacag
    11581 caggcacatg ccgtctgaag atgccggcaa aacgctcatc ctatccagca ggttcgacaa
    11641 caacacactg atgctggcag acaatatcaa ccaaggcgca ggcgcattgc agttcgacag
    11701 caacttcacc gtcgtcggta aaaaccacac atggcaaggt gcaggcgtta tcgtagccga
    11761 cggcaaacgc gtcttctggc aagtcagcaa ccccaaaggc gaccggctct ccaaactggg
    11821 cgcaggcacg cttatcgcca acggacaagg catcaaccag ggcgacatca gcatcgggga
    11881 aggcactgtc gtactcgccc aaaaagctgc ttcagacggc agcaaacaag cattcaacca
    11941 agtcggcatc accagcggca ggggcacggc cgtcctcgcc gacagccagc aaatcaaacc
    12001 cgaaaacctc tatttcggct tcaggggcgg acggctcgac ctcaacggca acaaccttgc
    12061 ctttacccat atccgccatg cggacggcgg cgcgcaaatc gtcaatcaca accctgacca
    12121 agccgcgaca ctgacgctga ccggcaaccc cgtcctcagt cctgagcatg tcgagtgggt
    12181 gcaatggggc aaccgtccgc aaggcaacgc ggcggtttac gaatacatca acccgcaccg
    12241 caaccgtcgg accgactact tcatactcaa acccggcggc aacccgcgcg aatttttccc
    12301 gttaaatatg gaaaactcaa caagctggca atttatcggc aacaacaggc aacaggccgc
    12361 caaacaagtc gcccaagccg aaaatgcccg ccccgaccta attaccttcg gcggatactt
    12421 gggtgagaac gcgcaaacgg gcaaagccgc accgagttac agcaaaacca atatcgaatg
    12481 ggtgaatagt ccaaacaccc cacacaagcc ctcgccctat acgaatacgc caacccgcac
    12541 aggggcaggc gtaccgacga ttaccgcctc aaagaaggcg gcaaccccgg cgcctacttc
    12601 ccatgggatg cctacagcac ccaagactgg gaatttctcg gacataaccg gcgaagcagc
    12661 catagaaaaa acccgccata tcgcaaatgc cgccgtatac ggccggcccg aataccgtta
    12721 caacggcgca ctcaacctgc actatcgtcc caaacgcacc gacagcacgc tgttgctcaa
    12781 cggcggcatg aaccttaacg gggaagtctt gattgagggc ggcaatatga ttgtgtcagg
    12841 caggcccgta ccccatgcct acgaccacca ggccaaacgc gaacccgttc ttgaaaacga
    12901 atggaccgac ggcagcttca aggctgcacg gttcaccctg cgaaaccatg cccgactgac
    12961 ggcagggcgc aataccgcgc atctggacgg cgacataacc gcatacgatc tgtccggcat
    13021 cgacctcggc tttacccaag gcaaaacacc ggaatgctac cgctcctacc atagcggcag
    13081 cacccactgc acacccaacg ccgttttaaa agccgaaaac tatcgtgcac tacctgcaac
    13141 gcaagtacgc ggcgacatta cccttaacga ccgttcagag ctccgcctgg gcaaagcaca
    13201 cctgtacggc agcatccgtg ccggcaaaaa caccgcagtc cgcatggaag cagacagcaa
    13261 ctggacactt tcccagtcca gccacaccgg cgcactgacg cttgacggcg cacaaattac
    13321 cctgaacccc gatttcgcca ataatacaca caacaaccgc ttcaacacac tgaccgtcaa
    13381 cggcacactt gacgggttcg gcacattccg attcctgacc ggcatcgtcc gaaaacaaaa
    13441 tgcccccctc aaactggaag gggacagccg cggcgcattc caaatccacg tcaaaaacac
    13501 cggacaagaa cctcaaacaa ccgaatcgct tgcacttgtg agcctcaatc cgaaacacag
    13561 ccaccaagcc cgattcaccc tccaaaacgg ctatgccgat ttgggtgcct accgctacat
    13621 cctccgcaaa aacaacaacg gatacagcct gtacaacccg ctcaaagagg ccgaacttca
    13681 aattgaagcc acgcgtgcgg aacatgagcg caaccaacag gcatacaacc aattacaggc
    13741 aaccgacatc agcagacagg ttcaacatga ctctgacgcg accaggcagg cactacaggc
    13801 ctggcagaac agtcaaaccg aacttgcccg catcgacagc caagtccaat atctgtccgc
    13861 ccaattgaaa cagacagacc cgctgaccgg cattctgacg cgtgcccaaa acctgtgtgc
    13921 cgcacaagga tacagtgtcg atatctgccg tcaggttgcc aaagccgccg acacgaacga
    13981 cctgacactc ttcgaaaccg aactggatac gtatatagaa cgtgtagaaa tggccgaatc
    14041 cgaacttgac aaagcacggc aaggcggcga tgcgcaagcc gtcgaaacag cccggcacgc
    14101 ctacctgaac gcactcaacc gtctgtcccg acaaatccac agtttgaaaa ccggcgttgc
    14161 cggcatccgt atgccgaacc tggccgaact gatcagccgg tcggccaaca ccgccgtttc
    14221 cgaacaggcc gcctacaata ccggccggca acaggcggga cgccgcatcg accgccacct
    14281 taccgatccg cagcagcaaa acatctggct ggaaaccggt acgcaacaaa ccgactacca
    14341 tagcggcaca caccgtccct accaacaaac taccaactat gcacatatcg gcatccaaac
    14401 cggcatcacc gaccgtctca gtgtcggtac aattttaacc gatgagcgca caaacaaccg
    14461 ttttgatgaa ggcgtatccg cccgaaaccg cagcaacggc gcacatctgt tcgtcaaagg
    14521 ggaaaacggc gcactctttg ccgcggcaga tttaggctac agcaacagcc gtacccgatt
    14581 taccgattat gacggggctg ccgtccgccg ccacgcatgg gatgcaggca tcaacaccgg
    14641 catcaaaatc gataccggca tcaacctcag accctatgcc ggcatccgta taaaccgcag
    14701 caacggcaac cggtacgtac tcgacggcgc agagataaac agcccggcgc aaatccaaac
    14761 cacatggcat gccggcatcc gtctcgataa aaccgtcgaa ctgggtcaag ccaagctgac
    14821 ccccgctttc agcagcgatt actaccatac ccgccaaaac agcggttccg ccctcagcgt
    14881 caacgaccgt accttactgc agcaagccgc ccacggcaca ctgcataccc tgcaaatcga
    14941 cgccggatac aaaggctgga acgccaaact tcatgccgct tacggcaaag acagcaacac
    15001 cgcccgccac aaacaggcag gaatcaaaat aggctacaac tggtaacaag ccgataaaaa
    15061 tgccgtctgg aacccgcgtt tcagacggca tttgcgttaa aaatagtaaa ccgttccaaa
    15121 agggagtaga atagtgccgt ttccaaccct gcgcctgtac cgtcaggctt ttattatgga
    15181 ccttcccagt tcgtttttac tgaacacccc ttccgattcc aaacgacaat aaccatcccg
    15241 ccggaatgcc tccccgcgca cggcgggcgg agcatttatg agcatcgaac caacccctcc
    15301 gaaccttgaa aacgacggta tcgaaaacga tgtagaacgc gtttccgccg atttcgaccg
    15361 tgtccactcc ctctgcgaaa tcctcgaacc tgcttttgaa caaatcgaaa acggcacacc
    15421 gctcgaagac gcgtcgctgc gcgacaagct gaccgagctg accgtcctct tgagcgagct
    15481 gcaccctgcc gacgtggcgg cggtattgga atcgctgccg ccgcgcgaac gcaatatcgt
    15541 ctggattctg gtcaaaccgg aagacgacgg cgaagtcttg ctggaagtgt ccgacgcggt
    15601 gcgcgaaacg ctgatcgagt cgatggacaa agacgaattg ttggcggcag ttgacaattt
    15661 ggacgcggac gaattggcgg aactggcaga cgatttgccg caccaagtgg tttatgaagc
    15721 cttgcagacg cgcgacgagg aagaacgcgc ccaagtcaaa gcggcaatgt cctacgaaga
    15781 caaccaagtc ggtgcgatta tggacttcga gttggtcagc atccgcgccg atgtcgcctg
    15841 cgaagtggtg ctgcgttacc tgcgccgctt cgacagcctg cccgaccata ccgacaagat
    15901 ttttgtcgtc gatgaaaacg acgtgctgca aggcgtgctg cccatccgca aacttttggt
    15961 cgccgacccc gaagacttgg tggaaaacgt gatggcgaaa gatgtcgtgc gtttccgcgc
    16021 cgaagacgac gtggaagaag cggcgcaggc attcgaacgc tatgacttgg ttaccgcgcc
    16081 cgtcgtcgat gaaaacaaaa agctcatcgg cagggtgacc gttgacgaaa tggtggacgt
    16141 gatccgcgaa gaatccgaag cggatatgtt gaacatggca ggtttgcagg aagaggaaga
    16201 cctgttcgcc cccgtcttgg attccgtcaa aaaccgctgg atgtggctcg ccgtcaacct
    16261 ctgcaccgcc ttcctcgcca gccgcgttat cggcgcgttt gaaggcagca tcgaaaaaat
    16321 cgtcgcactc gccgcgctga tgcccatcgt cgccggcatc ggcggcaact cgggcaacca
    16381 gacgattacc atgattgtcc gcgcgatggc gatggggcaa ctgacaggca tgcaggcagg
    16441 caggctgttg aaaaaagaag tcggtgtcgc cttggtcaac ggcatcattt ggggaacggt
    16501 catgggcgca gtatcttggc tgctttacgg cagcctcggc atcgggctgg ttatgattgc
    16561 cgcgatgacg ctcaacctcc tgctggcggc aaccgtcggc gtattaattc ccgtggtaat
    16621 ggaaaagttc ggacgcgatc ccgcgctggg cagctcggtg ctgattacgg cggtaacgga
    16681 ttccggcggc ttcctgattt tcttgggtct cgccaccctg ttcctgcttt aaatgccgtc
    16741 tgaacccgcg caaaaatgcc gtctgaagcg gaagctgctt cagacggcat ttgactattt
    16801 atccttgttg cacaagatta ttggacggta tgccggggca gccctttggc aacgccgacc
    16861 acatcctccc cgaacagcgc gttgacatcg gtttcgtcaa acacatattt gctgtggcag
    16921 aaatcgcaat cgacttcgat gctgccttgt tccaccacca cgccgccgac ttcttccccg
    16981 cccagcatca acagcatatc gctgactttg ccgcgcgaac aggtgcatga aaattcaaac
    17041 gtttccggct cgaacacgcg cggcggcgtt tcgtggaaca agcggtacaa aacatgttgc
    17101 gcatccaatt ctgccaactc gcccgccgtc agcgtgcgcg ccagcgtact gacgtgttcc
    17161 catgcctctt catccaacac ctcttcaggc agacgctgca ccagcagacc gcccgccgct
    17221 tcatcgcttg cagacaggac gatgtgcgta tcaagctgtt cggaacgttt catatagttc
    17281 atcaacattt gcgcgatacc gtcgccttcc aaaggcacta cgccctgcca gggttcgccg
    17341 tctttgggct gcagcgtcag cacgaatacg ccgccctcgc ccaaaagatc gaccaaactt
    17401 tcatcttcac cgatttctgc cgcctcgtcc caccgcgcgg ttgcacggac ggtacggtcg
    17461 gaagtcgctt ccgcaaccag cattttcagc cgcccctgcc cctgaacctg cacaatcagc
    17521 gtgccttcgt ttttgaggtt gcccgacagc agaacaccgg cagccaacag ctcgcccaag
    17581 gcgcggcgga tggcggcggg atagtttttc tgttttacaa tgtgctgcca cacgttttcc
    17641 agacggacgt gcagcccgcg cacgggcata tcgtcgaaga taaagcgggt acgcacatcg
    17701 gcgcggttga tggcggtttg attcatgatt ttctctgact gattgttcgg atggcggcta
    17761 tatggttgcg gtcggcgcga aaacaagacg gacggcggat gcgcttccca aattatcaat
    17821 aaattatata aaaatcaaca tattaactca atctaacaag ccgttttttg ccaaacagcc
    17881 gtttttttat atacaatcaa caagatattt tcgactgata cagcataaca tcgcacggcg
    17941 gcacgatgcc tcctgcgcgg aaacaccgat atggattctt ttttcaaacc ggcagtttgg
    18001 gcggttttgt ggctgatgtt tgccgtccgc cccgcccttg ccgacgagtt gaccaacctg
    18061 ctcagcagcc gcgagcagat tctcagacag tttgccgaag acgaacagcc cgttttaccc
    18121 atcaaccgag cccccgcccg gcgggcgggc aatgccgacg aactcatcgg cagcgcgatg
    18181 gggcttaacg aacagcccgt tttacccgtc aaccgagtcc ccgcccggcg ggcgggcaat
    18241 gccgacgaac tcatcggcaa cgcgatgggg cttaacgaac agcccgtttt acccgtcaac
    18301 cgagcccccg cccggcgggc gggcaatgcc gacgaactca tcggcaacgc gatgggactt
    18361 ttgggtattg cctaccgcta cggcggcaca tcggtttcta ccggttttga ctgcagcggc
    18421 ttcatgcagc acatctttaa acgcgccatg ggcatcaacc tgccgcgcac gtcggcagaa
    18481 caggcacgga tgggtacgcc ggttgcccga agcgaattgc agcccggaga tatggtgttt
    18541 ttccgcacgc tcggcggcag ccgcatttcc catgtcggac tttatatcgg caacaaccgc
    18601 ttcatccacg cgccgcgcac ggggaaaaat atcgaaatca ccagcctgag ccacaaatat
    18661 tggagcggca aatacgcgtt cgcccgccgg gtcaagaaaa acgacccgtc ccgctttctg
    18721 aactgatttc ccaaggaata cgcaatgagt atgcccgaaa tacccaaatg gtacgacgat
    18781 gacggacaga tcgtgtcctg taccgaaaag atcaaagtga tgtccgaaaa tatggccgag
    18841 ctgtatcaga cggcacaaga cgcgtttgaa gacgcgctgc tgatgggttg cggcgaacgt
    18901 cagttgcgcg attacctgct cgcgctgatt gaaggtttgg aaaatcccta ccgcaaagtc
    18961 tgaacacgcc ccggttgctg cggcacggtt tatccgtgcc gtttttgcgt ttgtgcgcgg
    19021 cttcggcttt tcagacggca tatttgacgt tatgattaaa cagttaacaa gatttatcac
    19081 aacgccgtca aaagacagac acacaacatg aacatcatcc gcgcgctcct catcattctc
    19141 ggctgcctcg ccgccggcga aaccgccgtt ttcctagcag gcatcaaact gcccggcagc
    19201 atcgtcggca tgggcgtgct gtttgcgctt ttgcaggcag gttgggtcaa aacgtcttgg
    19261 ctacaacagc ttaccgacgc gctgatggcg aatctgacgc tgtttctcgt gccgccctgc
    19321 gtggcggtca tcagctattt ggatttgatt gccgacgatt ggttttcgat actggtttcc
    19381 gcctccgcca gcactttgtg cgtactgctg gttacaggca aggttcaccg ctggatacgg
    19441 agcattatct gatgaacgaa atcctcaggc agcccagcgt tctgcttttc ctcacgcttg
    19501 ccgtgtacgc gcttgcgatt atcgtgcgca cgcgcacggg caatatcttc tgcaaccccg
    19561 tactcgtcag cactatcgtg ctgattgcct acctgaaaat cctcgacatc gattatgcgg
    19621 tgtaccacaa cgccgcgcaa ttcattgatt tttggctgaa acccgccgtc gtcgtgcttg
    19681 ccgtgccgct ctaccaaaac cgccgtaaaa tcttcaacca gtggctgccc gttatcgttt
    19741 cgcaacttgc aggcagcgtt acgggcattg ttacagggat gtattttgcc aaatggctgg
    19801 gcgcggaacg cgaagtcgtc ctctcgctcg cgtccaaatc tgttaccaac cccatcgcca
    19861 ttgaaatcac ccgctccatc ggcggcattc ccgccattac cgccgccacc gtcatcattg
    19921 ccggtctggt cggacagatt gccggttaca aaatgctgaa gaacacagtc gtcatgccct
    19981 cgtccgtggg tatgtcgctc ggcacggctt cgcacgcgat ggggattgcc gcctcgctcg
    20041 aacgcagccg ccgcatggcg gcatacgcgg gactggggct gacgttcaac ggcgtactga
    20101 ccgcgctgat tgcgccgctg ctcatccccg ttttgggatt ttgaacccgt ttcagacggc
    20161 atttcagccc atgctgtctg aacgccgaca cactcgcaag gagaaccgtt atggctgtca
    20221 acctgaccga aaaaaccgcc gaacaactgc ccgacatcga cggcattgcc ctctacaccg
    20281 cccaagcagg cgtgaagaag cccgggcata ccgacctgac actgattgcc gtagccgccg
    20341 gcagcaccgt cggtgcagtc ttcacgacca accgtttctg tgccgcgccc gtccacatcg
    20401 ccaaatcgca ccttttcgac gaagacggcg tgcgcgccct catcatcaac acgggcaacg
    20461 ccaacgcggg tacgggcgca cagggcagaa tcgatgcttt ggcagtgtgt gccgccgccg
    20521 cccggcaaat cggctgcaaa ccgaaccaag tgctgccctt ctccaccggc gtgattctcg
    20581 aaccgctgcc cgcagacaaa atcatcgccg cgctgcccaa aatgcagcct gccttctgga
    20641 acgaagcggc acgcgccatc atgaccaccg acaccgtgcc caaagccgcc tcgcgcgaag
    20701 gcaaggtcgg cgacaaacac accgtccgcg ccacgggcat cgccaaaggc tcgggcatga
    20761 ttcatcccaa tatggcgacc atgctcagtt tcatcgccac cgatgccaaa gtttcccaac
    20821 ccgtcctcca actgatgacg caggaaatcg ccgacgaaac cttcaacacc atcaccgttg
    20881 acggcgacac cagcaccaac gacagcttcg tcatcatcgc caccggcaaa aacagccaaa
    20941 gcgaaatcga caacatcgcc gacccgcgtt acgcccaact caaagaattg ttgtgcagcc
    21001 tcgcgctcga actcgcccaa gccatcgtcc gcgacggcga aggtgcgacc aagttcatca
    21061 ccgtccgcgt cgaaaacgcc aaaacccgcg acgaagcccg ccaagccgcc tacgccgtgg
    21121 cacgttcgcc gctggtcaaa accgcctttt tcgcctccga ccccaacctc ggcaggctgc
    21181 tcgccgccat cggttatgcc ggcgttgccg acctcgatac cgacctcgta gaaatgtatc
    21241 tcgacgatat tttggttgcc gaacacggtg gacgcgccgc aagctacacc gaagcacaag
    21301 ggcaggcggt gatgtcgaag gccgaaatca ccgtccgcat caagctgcat cgcggacaag
    21361 ccgccgccac cgtctatacc tgcgacctgt cgcacggata cgtttccatc aacgccgact
    21421 accgttcctg acccgacacg gcttcagacg gcatacataa aatgccgtct gaaccgccgg
    21481 acaacatacc atgacctcca cattcccccg ccgcctcgcc cgcaaaatcc gccaaacccg
    21541 ccgcctgtcg cgcaaaagca tcgcctttct gttccttttg gcaggttcgg cactcgtcgc
    21601 cctgaccgcg ctgttttttg cccatcttgc cgattttgcg ctggaactga acgccaaact
    21661 ggttcaacaa tacccgtggt tcgcgtgggt cgcgcttcct ttgggtttgc cgcttattgc
    21721 gtggctcaca cgcaaattcg cccccttcac cgccggcagc ggcatcccgc aggtcatcgc
    21781 ctcactgtcg ctgccctacg gcgcacagaa aacgcggctg atccgcctcg ggcagacgct
    21841 gctgaagatt ccgctgacct ttttgggtat gctgttcggc gcgtccatcg gacgcgaagg
    21901 tccgtccgtg caggtcggcg cggcagtgat gggcgcgtgg ggcgcgtggt gcaagaaaca
    21961 cggcttggca ttcaaaggga tgcaggaaaa cgatttgatg gcggcgggcg cggcgggcgg
    22021 tttggcagcc gcgttcaacg cgccgctggc gggcgtgatt ttcgccattg aggaactcgg
    22081 gcgcggcatc atgttgcgct gggagaggca aattcttttg ggcgtgctcg cctccggttt
    22141 catacaggtc gccattcagg gcaacaaccc gtatttttcc ggcttcaacg gcggcgtatt
    22201 ggaacatatc tttctgtggg tcgcactgtc cggcctggtt tgcggcgcgg cgggcgggct
    22261 gttcggacgt ttgctctatc gcggtgcggc ggcatttgca ccacgcaaga tacgcggctt
    22321 catccgcaac cgtccgctgc tgctggcggc actgatgggg ctgctgctcg ccctgctcgg
    22381 cacgttctac caaggcaaaa cctacggcac cggctaccac gaagccgccc aagccctgca
    22441 cggcatctat gaagccccct tcggactcgc cgccgccaaa tggctcgcca ccgtattcag
    22501 ctattgggca ggcgttccgg gcggcatttt cactccctcg ctgaccatag gcgcggtttt
    22561 gggcgagcat atcgccgcca tcgccgacat atcgcagggt gcaaacatca tcgtcctcat
    22621 ctgcatggcg gcatttctgg cgggcgcgac acaatccccg attacttccg ccgtcgtcgt
    22681 catggaaatg acgggcggac aaagcctgct gttttggatg ctaattgcct gcattttcgc
    22741 ctcgcaggtt tcgcgccagt tttcgccgcg tccgttctac cacgcatcgg gaatgcgctt
    22801 ccgccagcgc gtgcttcagg aaaccaccgc ccaaaccggc aatgcgcccg caagaccgca
    22861 aacagcaaac agcaaaacgg gaatgccgtc tgaaaattaa aacgcccccg atcaaacgcc
    22921 ggcagccgcc ttgatttgaa taccgttccg ccgccgcttg aaatttcagc aacaatgccg
    22981 tctgaacgac agaatgcggt tttcagacgg catttccaca tcccgatatt gcctaaacaa
    23041 aaccgaagcg tttgctataa ttctattttt taccgcacac gcaccaatca tgtttcccga
    23101 tttctcccaa accctctcca aagaccgcca cttcctacgt tccgccttca aaaatcccaa
    23161 caaatacggc gggttgtcca aagtcgaaga aaaataccga aaatcgcacg aaatcttttt
    23221 gaaacgtttg gcagccttgc ccaaacccga attcgacaac accctgcccg ttcacgaaaa
    23281 acttgacgaa atcaaaaaaa ccgttgccga acatcaggta acgattattt gcggcgaaac
    23341 cggttcgggc aagaccactc agttgcccaa gatttgtctg gaactcgggc gcggggcggc
    23401 gggtttgatc gggcataccc agccgcgccg tttggcggcg cgttccgtag cagagcggat
    23461 tgccgaagag ctgaaatccg aaatcggcag cgcggtcggc tataaagtgc gcttcaccga
    23521 ccacacctcg cgcgatgcct gcgtcaagct gatgaccgac ggcatcctgc tggcggaaac
    23581 ccagaccgac cgttatctcg ccgcctacga cacgattatc atcgacgaag cgcacgagcg
    23641 cagcctgaac atcgacttcc ttttgggcta tttgaaacaa ctcctgccgc gccgccccga
    23701 tttgaaagtc atcatcacct cggcaacgat agacgcagaa cgcttttccc aacacttcaa
    23761 cggcgcgccc gtgctggaag tgagcggacg cacctatccc gtcgaaatcc tctaccgacc
    23821 gctgatcggc aaagacgaag acgacgcgga aatagaattg accgacgcga ttgtcgatgc
    23881 ggcggacgaa ttggcgcgat atggcgaagg cgatattttg gtattcctgc cgggcgaacg
    23941 cgaaatccgc gaagccgccg aagccctgcg caaatccacg ctacgccgca acgacgaaat
    24001 cctgcccctg ttcgcacgcc tgtcgcacgc cgagcagcac aaaatctttc acccctccgg
    24061 agcgaaacgc cgcatcgtgc tggcaaccaa cgtcgccgaa acctcgctta ccgtgccggg
    24121 catcaaatac gtcatcgaca ccggcctcgc gcgcgtcaaa cgctattccg cacgggcgaa
    24181 agtggagcag cttcatatcg aaaaaatctc ccaagccgcc gcccgccaac gctccggccg
    24241 ctgcggacgc gtctccgcag gcgtatgtat ccgattgttt tcagaagaaa atttcaacaa
    24301 ccgccccgaa tttaccgacc ccgaaatcgc ccgcagcaac ctcgccgccg tcatcctgcg
    24361 catggcatcg ctgaaactcg gcgacgtagc ggcattccca tttttagaaa tgcccgattc
    24421 gaggtatatt aatgatgggt ttcaggtgtt gctggaatta ggggcagtgg aggccgcctg
    24481 aaaacaagta ggatatacac ttctacctga caaagaccaa caaacgataa aactacggca
    24541 catctagatg cccaacctac aacattgcac acggtaaaaa catgaaacac ttactcatcg
    24601 attttgaaaa cgtccagccg caaaacttag acaaattact aaccgaaaat acccatattt
    24661 ggctatttat aggtgtatta cacaaaatgt tacctattag tctggtgcaa tccctactac
    24721 gtttcggcga acgtgtccat cttgtccagt tacaaaaaac ggggaaaaac gcattggatt
    24781 tttacctgtc ctattacctc ggacaaatta ccgccacaga ccccaatgcc caaatcggca
    24841 tactctcgcg tgatggagga tacgatgttc tggtcgaaca tattttgaaa aaccaccagg
    24901 cgaagggtat cgtgcgccta gccaatatag atgaagtaca acatcagaaa attactaccg
    24961 aaccgccgtc agcattgctg gaaaacactc ctcagcctga aaccaccctc aaaccacagc
    25021 aaccattaac ttcctatttc caagcagccc taactgcact gcgccgcccc gacgctttcc
    25081 gcccctgccg cctgcataac ctgcgacaaa atctgcgtaa gcatattttg agtgatttgt
    25141 ttaaagaaaa aaccgatgaa gaatgcgaaa taaccactgc taacgttatc aataaactca
    25201 aagcacaaaa cttcatcagc attgatgaac aggaaaccgt ttcctaccat ctcagtgata
    25261 atgatttgtt acaaagaatc caacgccata ttttaagcca acgtcccaaa acctacgctg
    25321 attttcaagc cgtcgtgcaa aaccgagcag atgcacttca cttaacagtc ggtaccaacg
    25381 acattcaatc ctttgcgcga catttgcgcg accaaaacct gatccgccaa aacaatggga
    25441 aaattgaata tgcaccgttt actgaaccta aaccacagcc aacgcccaag cagcctaaaa
    25501 aaaccgcatg ggaacctgat gaaattattt ggaaaaaagt gattgccgcg ttatcgttaa
    25561 agaaccgtcc taataaaacc aaaactttac gcaatacaat ccaggcactc acaaaatcca
    25621 atgcacaaga aactgacaaa ctgctacaac atttacaaga tacccaagtc ctacgtattg
    25681 acggtactaa aattgtgtat atcaagtagg atgggcattt atgcctgacc tacaaaacca
    25741 aaaacaacct accgccctta atcaactcca caaacccttt tcagacaacc tcgttttttg
    25801 aaaaacaatc tgtaaacaga taactgctga agaataccgt tgccgagccc caaaacctgc
    25861 ctgcaacttt tattgtgaac ttcccattat gaaaaaatcc cttttcgttc tctttctcta
    25921 ttcatcccta cttaccgcca gcgaaatcgc ctatcgcttt gtattcggaa ttgaaacctt
    25981 accggctgca aaaatggcag aaacgtttgc gctgacattt atgattgctg cgctgtatct
    26041 gtttgcgcgt tataaggctt cgcggctgct gattgcggtg tttttcgcgt tcagcattat
    26101 tgccaacaat gtacattatg cggtttatca aagttggatg acgggcatca attattggct
    26161 gatgctgaaa gagattaccg aagtcggcag tgcgggcgcg tcgatgttgg ataagttgtg
    26221 gctgcctgcg ttgtggggcg tgttggaagt catgttgttt tgcagccttg ccaagttccg
    26281 ccgtaagacg catttttctg ccgatatact gtttgccttc ctaatgctga tgattttcgt
    26341 gcgttcgttc gacacgaaac aagagcacgg tatttcgccc aaaccgacat acagccgtat
    26401 caaagccaat tatttcagct tcggttattt tgtcggacgc gtattgccgt atcagttgtt
    26461 tgatttaagc aaaatccctg tgttcaaaca gtctgcgcca agcaaaatcg ggcagggcag
    26521 tgttcaaaat atcgtcctga ttatgggcga aagcgaaagc gcggcgcatt tgaaattgtt
    26581 tggctacggg cgcgaaactt cgccgttttt gacccagctt tcgcaagccg attttaagcc
    26641 gattgtgaaa caaagttatt ccgcaggctt tatgacggca gtatccctgc ccagtttctt
    26701 taacgtcata ccgcatgcca acggcttgga acaaatcagc ggcggcgata ccaatatgtt
    26761 ccgcctcgcc aaagagcagg gctatgagac gtatttttac agtgctcagg ctgaaaacca
    26821 aatggcaatt ttgaacttaa tcggtaagaa atggatagac catctgattc agccgacgca
    26881 gcttggctac ggcaacggcg acaatatgcc cgatgagaag ctgctgccgc tgttcgacaa
    26941 aatcaatttg cagcagggca ggcattttat cgtgttgcac caacgcggtt cgcacgcccc
    27001 atacggcgca ttgttgcagc ctcaagataa agtattcggc gaagccgata ttgtggataa
    27061 gtacgacaac accatccaca aaaccgacca aatgattcaa accgtattcg agcagctgca
    27121 aaagcagcct gacggcaact ggctgtttgc ctatacctcc gatcatggcc agtatgttcg
    27181 ccaagatatc tacaatcaag gcacggtgca gcccgacagc tatctcgtgc cgctggtgtt
    27241 gtacagcccg gataaggccg tgcaacaggc tgccaaccag gcttttgcgc cttgcgagat
    27301 tgccttccat cagcagcttt caacgttcct gattcacacg ttgggctacg atatgccggt
    27361 ttcaggttgt cgcgaaggct cggtaacggg caacctgatt acgggtgatg caggcagctt
    27421 gaacattcgc gacggcaagg cggaatatgt ttatccgcaa tgagtggcgt aaaaaccaat
    27481 aaagacaaat ttagatgatg tcggggaaga tgcccgaccg acaagactat gcaaaatatg
    27541 aaaaaccaag tacgcggatc aggcatggat gcacgatcca atccggccaa tgtttcagac
    27601 ggcctgcaaa acagttcggg tcatatcggt accaacacgc gttaccgcct gaccaaactc
    27661 ggcgaacaaa tggcgcgcct gcccatcgac ccgaaaatcg cgcgcatttt gttggcggcg
    27721 aagaagcacg actgcatggc ggaaatattg gtgatcgcgt ccgcgctgtc gattcaagac
    27781 ccgcgcgagc ggccgctgga agcgcgcgat gccgccgcca aggcgcacga gcgttttacc
    27841 gacaagcaat ctgactttct tgcctatctg aacatttggg acagcttcca gcgcgagcgc
    27901 gacaagggct tgtccaacaa gcagttggtg cagtggtgcc gtcaatattt cctgtcgcac
    27961 ctgcggatgc gcgagtggcg cgagctgcac caccagcttg cccaaaccgc gattgaaatg
    28021 ggtttgacca ccaaggaagc ggctttcaga cgactttccg aaatcaagca gcttacgtcg
    28081 tctgaaaacc aaggcgatca agacttatcc gccaaacgca aacaaaaaca actggataag
    28141 aaacaacacc gcgcccaaat ccgcgccgcc aaagaagcgg gctacgaaca aatccaccgc
    28201 gccctgctca ccggccttat cgccaacgtc ggcatgaaat cgcccgacgg caacgactat
    28261 accggcgcgc gcggcagccg tttccacctt ttccccgcct ccgccctgtt caaagccaaa
    28321 cccaaatggg tgatggcggc agaactcgtt gaaacgacaa aactttatgc gcgcgacgtc
    28381 gccgttatcc agcccgaatg gattgagcag gaagccccgc acctcgtccg ctaccactat
    28441 ttcgagccgc actgggaaca aaaacgcggc gaagtcgtcg ccagcgaacg cgtgacgctt
    28501 tacggcctga ccgtattgcc gcgccgcccc gtgccttacg gcaaagttgc gcccgaagaa
    28561 gcacgtgaaa tctttatccg cagcgcgttg gtggcgcagg aatgcgattt gaaagcggat
    28621 ttttttgtcc acaataaaaa gctgattaaa gaaattaccg aactcgaaca caaatcgcgc
    28681 aagcaagacg tattggtcga cgacgaagcc ctgtttgcgt tttataacga acgcctgccc
    28741 gaaatggctt ggaaagacgc gcaaggcagc gtttggggaa gtgaagattc cgtacggatt
    28801 attgaatctg acaaagccga gaggtcgtct gaaaatgagc gcaacgagtt tcgtaaaaac
    28861 aagcgtaatg ggtctcgcca aaatgaaaat cacggcaaca ccgtaggttg ggttgaaaac
    28921 ccaacatcag ccgcaactgc aaaaactgtt gggtttgaca acccaaccta cgctactcag
    28981 caacccaccc cctcccccgc gagggagggt cggggagagg gcaaaacagt tgccgcacaa
    29041 accaacttct ccgcaaccac agcaaaccct ctccctaacc ctctcccaca ggagggggaa
    29101 cagagtgccg cagcttcaac gatttcagac gacctgcatc ctgcaaatcc ccaacaaacc
    29161 gccctctccc ccgtggggga gggctgggga gagggcaaaa cagttgcctc tcaaaccaac
    29221 ttctccgcaa ccacagcaaa ccctctccct aaccctctcc cacaggaggg ggaacagagt
    29281 gccgcagctt cagcggtttc aaacgacccc aaacccaaaa agcagcctgc acccccaaaa
    29341 ggccgtctga aacccctccc cctcgccgac atccgtacct tccaagcctg gatcaaaacc
    29401 gccgaacgcg acaatctgcg cctgctgttc ctcagccgcg acgatttgat gcaacacgcc
    29461 gccgcacaca ttaccgaaga gcaattcccc aaattctggc aaaccgcaga cggcaagttc
    29521 aaactttcct accgcttcga gccgcaccat ccgctcgacg gcgttaccct caccctaccg
    29581 cttaccgtgc tcaaccgcat cagccccgcc gccctcgaat ggctcgtgcc cggtatgttg
    29641 cgcgagaaaa tccagctgct gattaaagca ctgcccaagc aaatccgccg catctgcgtg
    29701 cccgtacccg atttcatcac ccaattttta agccaaaacc ctgatcgcaa cgcccccatc
    29761 ctgccccaac ttgcccaagc catcgccaaa accgcaggcg acatccgcat actcgagcaa
    29821 atcaaccaag acgaatgggc cgcgttcagg ctgcccgaac actgctattt caacctccgc
    29881 attatcgacg acggcggaca agagcttgcc ggcggccgca aactgcacga attgcaacaa
    29941 cagctcggcc aagccgccgc cgtcaccttc cgcgacaaca cccaagaatt cgagcgcgac
    30001 aacgtgacca catgggacat cggaaccctg cccgaatcca tcaaattcgc ccgcggcaaa
    30061 caacagctca ccggctacct cggcctgcaa aaagaaaaag acggccgcat cgccctgcgc
    30121 ctgtttgaca ccaccgaagc cgccgaacag gcacaccgtc aaggcgtcat cgaattgatg
    30181 aagctgcaat taaaagagca ggtaaaagac ctgaacaaag gcatccaagg ctttacccaa
    30241 gccgccatgc tgctcaaaca catcaacgcc gacaccctgc gcgacgacct cacccaagcc
    30301 gtctgcgacc gcgcctttat cggcgaagac gagctgccgc gcaacgaaaa agccttcaaa
    30361 gaacaaatca aacgcgcccg tagccgcctg cccgccgtca aagaagccct cagccgctac
    30421 ctgcaggaaa ccgcagccgc ctacgccgaa ctcaacggca aactcggcaa acatccattg
    30481 acccacctcc taagactacg cctgcaaacc ctgcttgccc ccggcttcgc cacccgcacc
    30541 ccgtgggcac aatggccgcg cctccccatc tacctcaaag ccatgaccct gcgcctcgaa
    30601 aaatacagca gcaaccccgc ccgcgacgca gcccgcgaag ccgatatcca agagctggaa
    30661 caaatgtggc aggaaaaaac agacagcctg attaaacaag gtctccccat ttcagacggc
    30721 ctcgccgcgt ttaaatggat gattgaagaa ttgagggtgt cgctgttcgc gcaggaattg
    30781 aagacaccgt atccggtgtc ggtgaagcgg ttgttgaaag agtgggagaa aattttttaa
    30841 ctgaataatt ctgccgatca aaatttcttg acaaaacaaa ttttaatgaa tactataagt
    30901 agcattatga gtttagaaaa ttttcaatta ataaaaacac aattaggtgt tcccagttat
    30961 gaaaatgata gagttgcaat ctatcaagga gattgtatcc agttaatgga taagatttca
    31021 aaaggatcat tccaaatgac aatcacatct ccaccataca atattggtaa agaatatgag
    31081 tctattttag atttggaaca ttatcttgag tggtgtgaaa gatggatgca atcaatacat
    31141 cacttaactg aagatagcgg atgtttttgg ttaaatttag gatacttaca tgttgaaaaa
    31201 aagggactag cagttcctat tccttatctt ttatgggata aaactaactt tttcctttta
    31261 caagaggtgg tttggaatta tgcagctgga gttgcatgcc gtaatcgatt ttccccaaga
    31321 aatgaaaagt tattatggta tgtgaaaaat cctgctaaat atacattcaa tttagatgct
    31381 gtacgcgatc ccaacgttaa atatccaaat cagaaaaaaa atgggaaatt aaaatgtaat
    31441 cctctaggaa aaaacccaac ggatgtttgg caaatagcaa aagtcacttc tggaagaaat
    31501 aggtcatcga gagaaagaac tgctcatcct gcccaatttc cattggaatt aatagaaaga
    31561 ataatccgag caagtagcaa tcaaaatgat gtaatcttag atccatttat cggctcagga
    31621 acaaccgcct tagccggact aaaaaacaac cgttttgtca taggatttga aattaacgat
    31681 gagtatatag atattataaa agaacgcttc aaagagtatg atcttcaaaa tacttgatac
    31741 ttgtatataa cggtatagag gatagaccct cctctatact gtatttacaa tatttatttt
    31801 tgataaagta tttttagttt acttctataa gcctctggag agagcctcgc ctgctggcct
    31861 gtcgctgcag tttgagcaat ccaatccgta tgatctaacc aaccaaatct gataatttta
    31921 atttctggac gcaattttaa atcaggcata aatttttcaa aatttatagt tagataataa
    31981 ccatttttac tcttaccagt aattccatct tgtggttgtg cataactcct attaccaaat
    32041 atatattttg gatccgatga agtttttaat tctattgagt aatagtcgtc tttaatatat
    32101 acaatatctt tgtcatattt attcttttct ccttgccaat tttcaaatct agcctcaata
    32161 ttagcaggaa ttaaagcatg taataacgcc cctacaatct gaggagaagg aaaaaaattt
    32221 ttgccaattt gcaaattacc aattctagac gagaaaatat tttcccaact atctaaacaa
    32281 agttggacaa tctctttttc tgacaatgga tgctgcttaa tcagctcttc tgttttggaa
    32341 atccataact cctcattcaa agaagcatac ggcgatattt ttgatctatt catattattt
    32401 aatcccttct acaagttcag aaatattaat tgaaaaagac ttagctaaca gcgagatatt
    32461 caaaagactt atatttcttt ctccgcgttc aactgatcca atataagtac gatgtaatcc
    32521 aactttttct gctaattctt cttgtgacca accattaagt aaacgataat gttttacacg
    32581 cttaccaaac tcaactaatt cattttccat aatctgtatt attttaataa gtgaatactt
    32641 taagtctaca ctctataagt agcacactta acaaaccata tcctactgcg tgaataccat
    32701 agcaaactgt agcctgcatc aaaccgcgtg catgccttcg ggcacacact cggtctttct
    32761 aaaccactcc ccctttccac ccaaaccaaa ggtcgtctga aacccatttt cagacgacct
    32821 tttgccactt tgcaaagcaa gtattcccac caccctctcc ccaaatatcg cccaaaccag
    32881 taaacttccc atatttcaac aacttcttga aagccaacca tgtccggtat ctacctaccc
    32941 cacctatttc cgccccatat cgccgaacgc ggccttttgt attttcagca gggcaaggtt
    33001 cgcgatgtcc gaaaaacttc cgccgggcgt tatcgggcct agcgctgttt gctgccggcg
    33061 gtctgaataa tggtggcgtc aacgacggca gcggatgctt tctctacttt taggtttttt
    33121 tcggtcagtt ggcggttaat cagtttgagc aattcggaca gggtgtcgtc ttgcgccagc
    33181 cagttgcggt aacggcataa ggtgctgtaa tcggggatgc tcagttcgtc aaagcggcaa
    33241 aacaggttga agtcgatgcg ggtgatgagg ctgtgctcga gttcgggatc ggagaggctg
    33301 tgccattgtc cgagcagaac ggctttgaac atggacaaca gcgggtaggc gggacgaccg
    33361 cggtggtctc ggaggtaacg ggttttttga cggttcaggt attgttcgat cggttgccaa
    33421 tcaatcacct ggtccaactt caatagtggg aaacggtcga tgtgtttggc aatcatggct
    33481 tgggcggttt gctgaaagaa ggtgctcatg agaaatctcc taaatgtctt ggtgggaatt
    33541 taggggattt tggggagttt tgcaaaggtc tcagtctttt taaaccacag cccttcccaa
    33601 ctaaaccaaa aggtcgtctg aaccctattt tcagacgacc ttttgccact ttgtaaaaca
    33661 aatcttccca ccatcctctc cccaaacatc gcccgaacca gtaaacttct catatttcaa
    33721 caacttcttg gaagcaaacc atgccccata tccacccgct ccacctgttc ccgccccata
    33781 tcgccgaacg cggactgctc tattttcagc agggcaaagt tggtgctgtc caaaaaactt
    33841 ccaccgggcg ttatcgggcg gaggtatgcg gtttggaaaa ctattgggta tggctgaagc
    33901 tggatagtga tttgtatatt aaagacgaaa gctgcgattg cccttatgtc taaaggtgca
    33961 aacatacctt aaattactat attacatagg caaaatacaa gcctataacg aattggaaac
    34021 aaaatgccgt ctgaaagcat tataaaatct gttcaccttt tcagatgagt aatgtacacc
    34081 cctatacaat ttttgctact ataccccata aatccacggc taaagatatc cttattatgt
    34141 cctatgattt atcgaaacga cttgtaatcg gcttagcatc aagtgcccta ttcgacttat
    34201 ccgaatcgga taatatattt agaatggaag gggcagaaac ctataggcaa tatcagagag
    34261 aaaaacaaaa ccatccccta aaaaaggcgt tgtctttcca tttattaaaa aacttctgtc
    34321 aatcaatgaa ataaacccaa acgacccaac aattgggttt attcttttat ccagaaacaa
    34381 tccagataca gattacgagt cataactata ggcttaatat tacacgattc tcattccatc
    34441 aaggcggaaa accgcacaaa tactgaaaca ctatcgatcg atttgtaaac aagcctactt
    34501 aagtaacttg cagtccttat catttccttt aaaataatcc agcccgtcac tacacgaact
    34561 ggcggacttc ttgcaaataa aggttactag attttcattc atcttaataa taaaaggatt
    34621 tttatcttta tctatggcta ccgccttcaa catgaattta ctgtctaagg ccccgcgcgc
    34681 gattccattc aaacggatac aaaagccttc tgcctcttta atcggcaaac ttggccattt
    34741 ggtagatgtt tgtttaaatc tcccattctg cagataaaac ttttccataa aatgtgcatt
    34801 ttctaacaag gctgcccgca ctgcatttat ctttgctttc tcaacataat tgcgatagct
    34861 cggataaaca attaaagcaa gtacagacaa tatcaagacc actgatatta attcaaccag
    34921 cgtaaacccc cgattatcag tcattacttt acttccaata agaacagatt attcaacata
    34981 tttctttgaa cagacttact atcccattca gcagtatgca tatttcccac tctatttttt
    35041 atccgcgggc agcaccggtt tggctgggcc tttgggtgcg ggcgcaccga cggaagcctg
    35101 atccttcagc tttgccagca ccgcggggcc gatacccttt accttggtca aatcgtctac
    35161 agacttgaac gcaccgtttt gcgcacggta ttccgcaatg gccttcgcct tcgccgggcc
    35221 tatgcccggc agcgcctcca actcctgctg cgaagccgca ttgatgttta ccgccgcaag
    35281 ggagaaggcg caggagaaca gcatacagaa cagcacgaac attttcttca tggtttttcc
    35341 tttaagggtt gcaaacaata aaccgcatct tgcgacgata aaacgagtca ttctaaaatg
    35401 aatatcccaa agtttcaagc cgttcctccg caaacccgac cggacgccgt acggatgccg
    35461 tcccgccatc cccgacattt tttccgggta aagcaaaaac ccccgaataa tcgggggttt
    35521 tctgaaaggg tgtttggcag tgacctactt tcgcatggaa gaaccacact atcatcggcg
    35581 ctgagtcgtt tcacggtcct gttcgggatg ggaaggcgtg ggaccaactc gctatggccg
    35641 ccaaacttaa actgttacaa atcggtaaag ccttaatcaa tatattcggt aatgactgaa
    35701 tcagtcagta agcttttatc tcttgaagtt cttcaaatga tagagtcaag cctcacgagc
    35761 aattagtatg ggttagcttc acgcgttacc gcgcttccac accccaccta tcaacgtcct
    35821 ggtctcgaac gactctttag tgcggttaaa ccgcaaggga agtctcatct tcaggcgagt
    35881 ttcgcgctta gatgctttca gcgcttatct cttccgaact tagctacccg gctatgcaac
    35941 tggcgttaca accggtacac cagaggttcg tccactccgg tcctctcgta ctaggagcag
    36001 cccccgtcaa acttccaacg cccactgcag atagggacca aactgtctca cgacgtttta
    36061 aacccagctc acgtaccact ttaaatggcg aacagccata cccttgggac cgactacagc
    36121 cccaggatgt gatgagccga catcgaggtg ccaaactccg ccgtcgatat gaactcttgg
    36181 gcggaatcag cctgttatcc ccggagtacc ttttatccgt tgagcgatgg cccttccata
    36241 cagaaccacc ggatcactat gtcctgcttt cgcacctgct cgacttgtcg gtctcgcagt
    36301 taagctacct tttgccattg cactatcagt ccgatttccg accggactta ggtaaccttc
    36361 gaactcctcc gttacgcttt gggaggagac cgccccagtc aaactgccta ccatgcacgg
    36421 tccccgaccc ggatgacggg tctgggttag aacctcaaag acaccagggt ggtatttcaa
    36481 ggacggctcc acagagactg gcgtctctgc ttctaagcct cccacctatc ctacacaagt
    36541 gacttcaaag tccaatgcaa agctacagta aaggttcacg gggtctttcc gtctagcagc
    36601 gggtagattg catcttcaca accacttcaa cttcgctgag tctcaggagg agacagtgtg
    36661 gccatcgtta cgccattcgt gcgggtcgga acttacccga caaggaattt cgctacctta
    36721 ggaccgttat agttacggcc gccgtttact ggggcttcga tccgatgctc tcacatcttc
    36781 aattaacctt ccagcaccgg gcaggcgtca caccctatac gtccactttc gtgttagcag
    36841 agtgctgtgt ttttaataaa cagtcgcagc cacctattct ctgcgaccct ccggggctta
    36901 cggagcaagt ccttaacctt agagggcata ccttctcccg aagttacggt atcaatttgc
    36961 cgagttcctt ctcctgagtt ctctcaagcg ccttagaatt ctcatcctgc ccacctgtgt
    37021 cggtttgcgg tacggttcga ttcaaactga agcttagtgg cttttcctgg aagcgtggta
    37081 tcggttgctt cgtgtccgta gacactcgtc gtcacttctc ggtgttaaga agacccggat
    37141 ttgcctaagt cttccaccta ccggcttaaa caagctattc caacagcttg ccaacctaac
    37201 cttctccgtc cccacatcgc atttgaatca agtacaggaa tattaacctg tttcccatcg
    37261 actacgcatt tctgcctcgc cttaggggcc gactcaccct acgccgatga acgttgcgca
    37321 ggaaaccttg ggctttcggc gagcgggctt ttcacccgct ttatcgctac tcatgtcaac
    37381 attcgcactt ctgatacctc cagcacactt tacaatgcac cttcatcggc ctacagaacg
    37441 ctcccctacc atgccggtaa accggcatcc gcagcttcgg ttatagattt gagccccgtt
    37501 acatcttccg cgcaggacga ctcgaccagt gagctattac gctttcttta aatgatggct
    37561 gcttctaagc caacatcctg gctgtctggg ccttcccact tcgtttacca cttaatctat
    37621 catttgggac cttagctggc ggtctgggtt gtttccctct tgacaacgga cgttagcacc
    37681 cgctgtctgt ctcccgagga accacttgat ggtattctta gtttgccatg ggttggtaag
    37741 ttgcaataac cccctagcca taacagtgct ttacccccat cagtgtcttg ctcgaggcac
    37801 tacctaaata gttttcgggg agaaccagct atctccgagt ttgtttagcc tttcacccct
    37861 atccacagct catccccgca ttttgcaaca tgcgtgggtt cggtcctcca gtacctgtta
    37921 cggcaccttc aacctggcca tggatagatc actcggtttc gggtctacac ccagcaactc
    37981 atcgccctat taagactcgg tttccctacg cctcccctat tcggttaagc tcgctactga
    38041 atgtaagtcg ttgacccatt atacaaaagg tacgcagtca caccactagg gcgctcccac
    38101 tgtttgtatg catcaggttt caggttctgt ttcactcccc tcccggggtt cttttcgcct
    38161 ttccctcacg gtactggttc actatcggtc gatgatgagt atttagcctt ggaggatggt
    38221 ccccccatat tcagacagga tttcacgtgt cccgccctac ttttcgtacg cttagtaccg
    38281 ctgttgagat ttcgaatacg ggactgtcac ccactatggt caagcttccc agcttgttct
    38341 tctatctcga cagttattac gtacaggctc ctccgcgttc gctcgccact acttgcggaa
    38401 tctcggttga tttcttttcc tccgggtact tagatggttc agttctccgg gttcgcttct
    38461 ctaagtctat gtattcaact taggatactg cacagaatgc agtgggtttc cccattcgga
    38521 catcgcggga tcattgcttt attgccagct cccccgcgct tttcgcaggc ttacacgtcc
    38581 ttcgtcgcct atcatcgcca aggcatccac ctgatgcact tattcacttg actctatcat
    38641 ttcaagaact tctttgactt tgcctaacat tccgttgact agaacatcag acttgaattt
    38701 cctactttga taaagcttac tgctttgttg tgtcttaatc ctgccttttg tgtttcagga
    38761 ttaagtcgat acaatcatca cccaaatact gtgtttgttt tcttttctct cgcgagagat
    38821 ttttatcctt tgcaaagaat aaaaaatcaa aacaaacgct ttgtctttgt ttgttgattt
    38881 cggctttcca atttgttaaa gatcgatgcg ttcgatattg ctatctactg tgcaaatcaa
    38941 aacgagctga ttattatatc agcattttgt tcttggtcaa gtgtgacgtc gccctgaatg
    39001 gattctgttc cattcttccg ttttgatttg tacagtattg gtggaggcaa acgggatcga
    39061 accgatgacc ccctgcttgc aaagcaggtg ctctaccaac tgagctatgc ccccgttctt
    39121 ggtgggtctg ggaggacttg aacctccgac cccacgctta tcaagcgtgt gctctaacca
    39181 gctgagctac aaacccggat tctcttctta agcgaatctt gccttcactc aagcttcttc
    39241 cgcatctttt tcagtttacc gataagtgtg aatgcctaaa gcctcttctt tctctagaaa
    39301 ggaggtgatc cagccgcagg ttcccctacg gctaccttgt tacgacttca ccccagtcat
    39361 gaagcatacc gtggtaagcg gaccccttgc ggttatccta cctacttctg gtatccccca
    39421 ctcccatggt gtgacgggcg gtgtgtacaa gacccgggaa cgtattcacc gcagtatgct
    39481 gacctgcgat tactagcgat tccgacttca tgcactcgag ttgcagagtg caatccggac
    39541 tacgatcggt tttgtgagat tggctccgcc tcgcggcttg gctaccctct gtaccgacca
    39601 ttgtatgacg tgtgaagccc tggtcataag ggccatgagg acttgacgtc atccccacct
    39661 tcctccggct tgtcaccggc agtctcatta gagtgcccaa ctgaatgatg gcaactaatg
    39721 acaagggttg cgctcgttgc gggacttaac ccaacatctc acgacacgag ctgacgacag
    39781 ccatgcagca cctgtgttac ggctcccgaa ggcactcctc cgtctccaga ggattccgta
    39841 catgtcaaga ccaggtaagg ttcttcgcgt tgcatcgaat taatccacat catccaccgc
    39901 ttgtgcgggt ccccgtcaat tcctttgagt tttaatcttg cgaccgtact ccccaggcgg
    39961 tcaatttcac gcgttagcta cgctactaag caatcaagtt gcccaacagc taattgacat
    40021 cgtttagggc gtggactacc agggtatcta atcctgtttg ctacccacgc tttcgggcat
    40081 gaacgtcagt gttatcccag gaggctgcct tcgccatcgg tattcctcca catctctacg
    40141 catttcactg ctacacgtgg aattctacct ccctctgaca cactcgagtc acccagttca
    40201 gaacgcagtt cccgggttga gcccggggat ttcacatcct gcttaagtaa ccgtctgcac
    40261 ccgctttacg cccagtaatt ccgattaacg ctcgcaccct acgtattacc gcggctgctg
    40321 gcacgtagtt agccggtgct tattcttcag gtaccgtcat cagccgctga tattagcaac
    40381 agccttttct tccctgacaa aagtccttta caacccgaag gccttcttca gacacgcggc
    40441 atggctggat caggcttgcg cccattgtcc aaaattcccc actgctgcct cccgtaggag
    40501 tctgggccgt gtctcagtcc cagtgtggcg gatcatcctc tcagacccgc tactgatcgt
    40561 cgccttggta ggcctttacc ccaccaacca gctaatcaga tatcggccgc tcgaatagcg
    40621 caaggcccaa aggtcccctg ctttctctct caagacgtat gcggtattag ctgatctttc
    40681 gatcagttat cccccactac tcggtacgtt ccgatatgtt actcacccgt tcgccactcg
    40741 ccacccgaga agcaagcttc tctgtgctgc cgtccgactt gcatgtgtaa agcatgccgc
    40801 cagcgttcaa tctgagccag gatcaaactc ttatgttcaa tctctaactt tttaacttct
    40861 ggtctgcttc aaagaaacca acaggacaat gttcaaaaca ttatcttgtc tgtctttcaa
    40921 acagtgtgag gctcaaggca ctcacactta tcggtaatct gttttgttaa agagcgttgc
    40981 gaattataaa gcatcccttc cgcctgtcaa gatatctctc gatatcccca acattctgtg
    41041 ctatactttt cagttcgtcc gccacttctg cagcagcgaa gaaccgaact atacgcccac
    41101 agggaaaaac ggtcaatgct tttctgaaga aattttttta aaaatattta tctatttgtt
    41161 tataaattta atttatatca gtcaatttta ttttccatac agaattcttc cagtgcccga
    41221 tggatatttt cagtctgcca ttcgtttttt aagggtgcaa caatttcgat ttgtcggttt
    41281 tggtagtcaa attgtatttt ccatgcatac agaaacatgg tttcggattc tgttccgccg
    41341 tataagctgt cgcccagaat cggactgccc aaacttttca tcgccactct caattggtgc
    41401 gttttgcccg tatgcggttc taggatgaac agccgcagtt tttcggcgat actgatgctg
    41461 tggaatcggg taacggcgat attttctgta ttgcgcgtca acttccacat tccacatctg
    41521 gatttttcca ttccgccttt aatccaaccc tgctttttgg acggcttgcg gtcggacagt
    41581 gccaaatagg tttttttgat gcttttgccg gcaaactgtc cgctaagggc ggacgcgctt
    41641 tctttgttga gggcaaacag taaaatgccg ctggtctgtt tgtccaatcg gtgcagcagc
    41701 cacacacgct ctacgcccaa ctgtatggcg agtgttcggg ccagtccggt ctcgccgctg
    41761 tcttggtgga cggatatgcc gcccggtttg ttgatggcga cgaagtcttg atggcggaac
    41821 aaaatttcca acatatccat atatgccttg caaaaataga agggttcaat tttcgtgttg
    41881 atgttcggca aggatttttt cgtacacagc ttgcggcacg tagcggtgga tcgtttccgt
    41941 ccagccttcc ggcccgacca gtcctttgac catagtggac gacacttcgg cgatttcgcg
    42001 cggcggcatg aggaatacgg tggatatttc gggggcgagg tcgctgttga tatggcgcat
    42061 ggaacgttcg tattcgtaat ccgaagcaga acggatgccg cgcacgatga atcctgcatc
    42121 tacctcacgg gcgtaatgca ccagaaatcg gttttcaaat acatcggttc tgacgttggg
    42181 aaacatttta gtaatatcgc acaacatatc ctgcctttca gcgacggtat aggtgctgcg
    42241 tttgtcgggg ttaatgccga tggcgacgat gagttcgtca aacatagatt gcgcctgccg
    42301 tatcatccac agatgcccca atgtgggcgg atcgaaactg ccggcataaa cggcgcggcg
    42361 cggtgtattc ggtaacattt gattcctccc ggtttcatag ccggctgt
//
LOCUS       DAWKQJ010000003        36847 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000003 DAWKQJ010000000
VERSION     DAWKQJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36847
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.003"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(162..2024)
                     /gene="hscA"
                     /locus_tag="WJL20_000092"
     CDS             complement(162..2024)
                     /gene="hscA"
                     /locus_tag="WJL20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ7682308.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGCVTLPSVVRYLENGGIEVGKTALSAQKSDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRIIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2195..3067
                     /gene="hpnD"
                     /locus_tag="WJL20_000093"
     CDS             2195..3067
                     /gene="hpnD"
                     /locus_tag="WJL20_000093"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ7682309.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGKMPEHPVNQALRQVKETFMLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCHCVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYCE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3465..4778
                     /locus_tag="WJL20_000094"
     CDS             3465..4778
                     /locus_tag="WJL20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ7682310.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPHAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            4861..5580
                     /locus_tag="WJL20_000095"
     CDS             4861..5580
                     /locus_tag="WJL20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7682311.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5745..6416
                     /locus_tag="WJL20_000096"
     CDS             5745..6416
                     /locus_tag="WJL20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ7682312.1"
                     /translation="MKTVSTAVVLAAAAVSLPGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6426..6794
                     /locus_tag="WJL20_000097"
     CDS             6426..6794
                     /locus_tag="WJL20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ7682313.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6791..7438
                     /locus_tag="WJL20_000098"
     CDS             6791..7438
                     /locus_tag="WJL20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ7682314.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            7880..9023
                     /locus_tag="WJL20_000099"
                     /pseudo
     CDS             7880..9023
                     /locus_tag="WJL20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9133..9342)
                     /locus_tag="WJL20_000100"
     CDS             complement(9133..9342)
                     /locus_tag="WJL20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682315.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(9339..9635)
                     /locus_tag="WJL20_000101"
     CDS             complement(9339..9635)
                     /locus_tag="WJL20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ7682316.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(9614..10219)
                     /locus_tag="WJL20_000102"
     CDS             complement(9614..10219)
                     /locus_tag="WJL20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682317.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            10299..10586
                     /locus_tag="WJL20_000103"
     CDS             10299..10586
                     /locus_tag="WJL20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ7682318.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            10558..10860
                     /locus_tag="WJL20_000104"
     CDS             10558..10860
                     /locus_tag="WJL20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ7682319.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(11543..13516)
                     /locus_tag="WJL20_000105"
     CDS             complement(11543..13516)
                     /locus_tag="WJL20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ7682320.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGAAADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIKGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPAGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(13527..14087)
                     /locus_tag="WJL20_000106"
     CDS             complement(13527..14087)
                     /locus_tag="WJL20_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7682321.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPSSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(14084..15139)
                     /locus_tag="WJL20_000107"
     CDS             complement(14084..15139)
                     /locus_tag="WJL20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7682322.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADELGAAANVRDGEAQLMAAPAGVSTECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDDAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            complement(15153..15500)
                     /locus_tag="WJL20_000108"
     CDS             complement(15153..15500)
                     /locus_tag="WJL20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ7682323.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(15600..16268)
                     /locus_tag="WJL20_000109"
     CDS             complement(15600..16268)
                     /locus_tag="WJL20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ7682324.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(16265..17410)
                     /locus_tag="WJL20_000110"
     CDS             complement(16265..17410)
                     /locus_tag="WJL20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7682325.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(17400..18731)
                     /locus_tag="WJL20_000111"
     CDS             complement(17400..18731)
                     /locus_tag="WJL20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7682326.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(18731..20902)
                     /locus_tag="WJL20_000112"
     CDS             complement(18731..20902)
                     /locus_tag="WJL20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ7682327.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            21057..21659
                     /locus_tag="WJL20_000113"
     CDS             21057..21659
                     /locus_tag="WJL20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682328.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(21703..21972)
                     /locus_tag="WJL20_000114"
     CDS             complement(21703..21972)
                     /locus_tag="WJL20_000114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682329.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(21912..22277)
                     /locus_tag="WJL20_000115"
     CDS             complement(21912..22277)
                     /locus_tag="WJL20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682330.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(22281..22658)
                     /locus_tag="WJL20_000116"
     CDS             complement(22281..22658)
                     /locus_tag="WJL20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7682331.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(22723..24132)
                     /locus_tag="WJL20_000117"
     CDS             complement(22723..24132)
                     /locus_tag="WJL20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ7682332.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(24135..24332)
                     /locus_tag="WJL20_000118"
     CDS             complement(24135..24332)
                     /locus_tag="WJL20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7682333.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(24329..24979)
                     /locus_tag="WJL20_000119"
     CDS             complement(24329..24979)
                     /locus_tag="WJL20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7682334.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(24969..25391)
                     /locus_tag="WJL20_000120"
     CDS             complement(24969..25391)
                     /locus_tag="WJL20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7682335.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(25391..25789)
                     /locus_tag="WJL20_000121"
     CDS             complement(25391..25789)
                     /locus_tag="WJL20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682336.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(25854..26762)
                     /locus_tag="WJL20_000122"
     CDS             complement(25854..26762)
                     /locus_tag="WJL20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7682337.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(26762..27823)
                     /locus_tag="WJL20_000123"
     CDS             complement(26762..27823)
                     /locus_tag="WJL20_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682338.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPEGAALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(28054..28470)
                     /locus_tag="WJL20_000124"
     CDS             complement(28054..28470)
                     /locus_tag="WJL20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7682339.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(28598..29923)
                     /locus_tag="WJL20_000125"
     CDS             complement(28598..29923)
                     /locus_tag="WJL20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ7682340.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(29910..31478)
                     /locus_tag="WJL20_000126"
     CDS             complement(29910..31478)
                     /locus_tag="WJL20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7682341.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(31478..33100)
                     /gene="terL"
                     /locus_tag="WJL20_000127"
     CDS             complement(31478..33100)
                     /gene="terL"
                     /locus_tag="WJL20_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7682342.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            33112..33624
                     /locus_tag="WJL20_000128"
     CDS             33112..33624
                     /locus_tag="WJL20_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682343.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            complement(33621..34127)
                     /locus_tag="WJL20_000129"
     CDS             complement(33621..34127)
                     /locus_tag="WJL20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7682344.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(34130..34327)
                     /locus_tag="WJL20_000130"
     CDS             complement(34130..34327)
                     /locus_tag="WJL20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682345.1"
                     /translation="MSAPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(34324..34590)
                     /locus_tag="WJL20_000131"
     CDS             complement(34324..34590)
                     /locus_tag="WJL20_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682346.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(34602..34940)
                     /locus_tag="WJL20_000132"
     CDS             complement(34602..34940)
                     /locus_tag="WJL20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682347.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(34937..35155)
                     /locus_tag="WJL20_000133"
     CDS             complement(34937..35155)
                     /locus_tag="WJL20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682348.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            35168..35338
                     /locus_tag="WJL20_000134"
     CDS             35168..35338
                     /locus_tag="WJL20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ7682349.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(35383..35688)
                     /locus_tag="WJL20_000135"
     CDS             complement(35383..35688)
                     /locus_tag="WJL20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682350.1"
                     /translation="MSLLKTVKMQATVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(35585..35875)
                     /locus_tag="WJL20_000136"
                     /pseudo
     CDS             complement(35585..35875)
                     /locus_tag="WJL20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35910..36074)
                     /locus_tag="WJL20_000137"
     CDS             complement(35910..36074)
                     /locus_tag="WJL20_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682351.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(36077..36283)
                     /locus_tag="WJL20_000138"
     CDS             complement(36077..36283)
                     /locus_tag="WJL20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682352.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(36280..36822)
                     /locus_tag="WJL20_000139"
     CDS             complement(36280..36822)
                     /locus_tag="WJL20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7682353.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
BASE COUNT         7698 a        10377 c         9716 g         9056 t
ORIGIN      
        1 caacgctgta ccggtttaaa tttaattcac tatacacgcc cttgatgacg gttttcatcg
       61 aatagtcaat acctgacttt tccatatcga tgtttttcaa gtgttttcga gccttcagac
      121 ggcatcaggt tatatctatg ccgtctgaaa ccgtttaagt atcaaatatt atcgacactc
      181 tggcctgtca atgcgcgttg gatattgcgg ttcatgcgtt tggcggcgaa gttgtcggtg
      241 ctgcggctga gtttggcgac ggcggagcgg atgtcttcgg cttttccgtc tttcagacgg
      301 ccttgcaaat cggcgatgcc ttgccggatc tgttgcaatt ctttggcatc cagtaaatcg
      361 ctgtccaact cgagggcggc gttgacggca tcggtcaggc tttcggcttc gactacggct
      421 tcggcgcggg cgcgtgccgc catatcttcg gcggcgttgt ccatactgtc tttaagcatt
      481 cgggtaatcg cgccgtcgtc caagccgtag gaaggtttga cttcgatttg cgcctgtacg
      541 ccggtgcttt gttcttgggc ggaaacggac agcagcccgt ccgcatcgat ttggaaggtt
      601 acgcggatac gcgccgcacc cgccgccatc ggtggaatgc cgcgcagggt gaatttggca
      661 aggctgcggc agtcagaaac gagctcgcgc tcgccttgca cgacgtgtat cgtcatcgcg
      721 gtttgaccgt ctttgaaggt ggtaaagtcc tgcgcgcgcg cggtggggat ggtggaattg
      781 cgcgggatga ttttttccgc caagccgccg taggtttcca aaccgagcga caaaggtgta
      841 acgtccagca gcagccattc gccgtcggtt ttgttgcctg cgaggacgtt tgcctgtatg
      901 gcggcgccga gcgcgacgac ttcgtcggga ttgaggttgt tcagcggggt ttgtccgaag
      961 aaggtggcga ctgcctgttg gacgtgcagc atacgggtcg aaccgccgac cataatcacg
     1021 cctttgactt cgtttttacc gacaccggca tctttcaacg cctgtgtgac cggttcgagc
     1081 gttttcatca ccaaatgctg cgtcaggttg tggaactcgg cgcgactgat gcttgtgtcg
     1141 attgccatgc cgtctgaaag cgtcgcctga atgcgcgctt cggtttgcgt ggttaattgt
     1201 tctttggcgg cgcggacgag cgagagcagg agttggctgt cttgttcgtt gagttgggag
     1261 agtccgtttt gttcgagcag gtagcagaac aaacgatggt cgaaatcatc gccgcccaac
     1321 gcgctgttgc cgccggtggc tttgacttca aacagtcctt tggtcagttg caatacggat
     1381 acgtcgaatg tgccgccacc caagtcgtac acgacaaacg tgccttccga ggcgttgtcc
     1441 agcccgtagg cgattgcggc ggcggtgggt tcgttgagca ggcgcaatac gttcaaaccg
     1501 gccagacgcg cagcatcttt ggtggcttgg cgttgggcat catcgaaata ggcgggaacg
     1561 gtaatcacca cgccgaccaa atcgccaccc aaggtttctt cggcacgcga tttaagggtt
     1621 ttgaggattt ccgccgacac ttcgacaggc gttttcaccc cctgccgcgt atgcagttcg
     1681 ataatgcgtt gattgtcgcc gaaacggtaa ggcaggtaat gcgtattttg atgcagatcg
     1741 gcaagcgtcc gtccgataag gcgtttggcg gagctgacgg tgttcagcgg gtcggatttt
     1801 tgcgcggaca gggcggtttt gccgacttca atgccgccgt tttccaaata acggacgacg
     1861 gaaggcaggg taacgcaccc gtcggcatcg ggcaggcagg ccgcactgcc gctgcggacg
     1921 gtggcaacca agctgttggt cgtacccaaa tcgatgcctg ccgccaaacg gtgccggtgc
     1981 ggagcggcgg acatacccgg ttctgaaatc tgcaaaagag ccataaattc tgcctgtcgg
     2041 tgcggtgtgt aaaggaagga aagcggcatt ttaacagaat ttgcgccgat agttgagcaa
     2101 accgctaagg attgagccgt gccgccgaaa ttgccctcta ccgccaaatg ccgataaaat
     2161 ccgcctttcc cgcccatcat taggaatata aatcgtgaaa ggacttgact attgccgtca
     2221 aaaagcagaa gaaagccgct ccagtttttt gtcgggcttc cgtttcctga ctcaggaaaa
     2281 acgggatgcg gtaacggttc tgtatgcctt ttgccgcgaa ttggacgatg tggttgacga
     2341 atgttccaac cccgatgttg cacaggcgac attgaactgg tggcgcggcg atttggacaa
     2401 ggtgttcggc ggcaagatgc cggaacaccc cgtcaatcag gccttgcggc aggttaaaga
     2461 aaccttcatg ctgccgaaat atgaactgga agccttaatc gacgggatgc agatggattt
     2521 ggttcgggcg cgttacggca gttttgaaga attgaaactg tattgccact gcgtcgcggg
     2581 cgtagtcggc tgcctgattg cgcggatttt ggggttttca gacggcaaaa cactagaata
     2641 cgccgacaag atgggacttg cgctgcaact gaccaacatc atccgcgatg tcggcgaaga
     2701 cgcgcgcagg ggacggattt acctgccgat ggaggaaatg cggcggtttg acgtacccgc
     2761 aagcgtgatt ttgcaatgca gcccgacggg caattttgcc gaattgatgg cgttccaaat
     2821 caaacgcgcc cgtgaaacct actgcgaagc cgtatcgctg ctgccggctg ccgataaaaa
     2881 agcccaaaaa gtcggactgg ttatggcggc ggtttattac gagctattga acgaaatcga
     2941 ccgggacggc gcgcaaaacg tcctcaaata caaaatcgcc ctcccttcgc cgcgcaaaaa
     3001 acgcattgcc ctgaaaacct ggttattcgg attcaaaccg cgccccggca cgccggaacg
     3061 ggcataaggc gcataccgcc cgccgcccgt caggcaaacc cgattcccac agccccgccg
     3121 gacggggttt caaccatcgt ctgaaagtct gcatcgtaac cgcgcaatcc cgttggcgtt
     3181 cgcgctttga aatcgaattg ctgggaattt aaacaggagc agaagtaaat gaaagcagtc
     3241 agggatatag ccttatggct ggcagtaacg gtttggatca actttttccc cgacaattac
     3301 cgctatgata ccgttccaca gggacggtac ggatactggc atgcagacca tccgtggtat
     3361 ccctatgcgc gctttctgct gcctttattt ttagcctgta tccttttcta ccgtcatttt
     3421 cggaaacgga aatagatacg gctgcaaaac cattggagga ataaatgatg aacacgccgc
     3481 atccgcgccc gaaaatcgcc gtcatcggcg caggctgggc cggcttgtcc gccgccgtta
     3541 ccttggcgcg gcacgccgac gttaccctgt ttgaagccgg ccggcaggcg ggcggcaggg
     3601 cgcgcgcact ggccggaaat accgacggtt tcggtttttt ggacaacggg cagcatattt
     3661 tactcggcgc ataccggggc gtgttgcgcc tgatgaaaac catcggttca gacccccatg
     3721 ccgccttttt gcgcgtaccg ctgcactggc atatgcacgg cggtttgcag ttccgcgccc
     3781 tccccctgcc cgcgcccctg catattttgg gcggcgtgct gcttgcccgg cgcgtaccgt
     3841 ccgcattcaa agccaaactg cttgccgata tgtccgattt gcagaagtcc gcacgcctcg
     3901 gacagcccga cacgacagtg gcgcaatggc tgaaacagcg gaacgtgccg cgtgccgccg
     3961 tgatgcagtt ttggcagccc ttggtttggg gcgcgctcaa cacgcctttg gaaaccgcaa
     4021 gcctgcgcgt gttgtgcaac gttttgtccg acggcgtgct gacgaaaaaa tccggcagcg
     4081 actatctcct acccaagcag gatttgggcg caatcgtcgc cgaacctgcc ttggcggagc
     4141 ttcaacggct cggcgcggac atccgcctcg aaacgcgcgt atgccgtctg aaccccctcc
     4201 cggacgggaa agtgctcgtc aacggcgaag ctttcgatgc cgccgtcccc gccaccgcgc
     4261 cctaccacgc cgccgcgctc ctgcccgaag gcacgcccga acacgttcag acggcatatc
     4321 aaaaccttcg ctaccacgcc atcaccaccg tctatctgcg ctacgccgaa cccgtccgcc
     4381 tgcccgcccc gctgaccggc cttgccgacg gcacggtgca atggctgctt tgccggggca
     4441 ggctcggact gcctgaaaac gaagtgtccg ccgtcatcag cgtttccgac cgcgtcggcg
     4501 cgtttacaaa ccgagcgtgg gcggacaaag ttcacgccga cctcaaacgc atccttccgc
     4561 atttgggcga acccgaagcc gtgcgcgtca tcaccgaaaa acgcgccaca accgcagccg
     4621 atgccccgcc gccggatttg tcgtggttgc accggcaccg catcttcccc gccggcgact
     4681 acctccaccc agactacccc gccacgctcg aagccgccgt acaatcaggt ttcgcgtcgg
     4741 cggaagcctg cctgcaaagc ctgagcgatg ccgtctgaaa acgccggccg gcatcgggac
     4801 gctttacaag gtgcggcaaa acgctaaaat accttaaccg accaccaaaa aggaaaaagt
     4861 atggcgacac tgtccgacaa aaccatctta gtaaccggcg catcgcaagg tctgggcgaa
     4921 caggtcgcca aagcctatgc ggcggcaggc gcaaccgtga ttctggtggc acgccatcag
     4981 aaaaaactgg aaaaagtcta tgacgcgatt gtcgaagccg gacaccccga acccttcgcc
     5041 atccgtttcg acctgatgag tgcggaagaa aaagaattca atcatttcgc cgccaccatt
     5101 gccgaagcca cgcaaggcaa actggacggc atcgtccact gcgccggcta tttttacgcc
     5161 ctctcgccgc tggatttcca aaccgtcgcc gaatgggtca accaataccg catcaacacc
     5221 gtcgcaccta tggggctgac ccgcgccctg ttcccgctgc tgaagcagtc gcccgacgcg
     5281 tccgtcatct tcgtcggcga aagccacggc gaaacaccca aagcctactg gggcggcttc
     5341 ggcgcgtcca aagccgcgtt gaactacctg tgcaaagtcg ccgccgacga atgggaacgc
     5401 ttcggcaacc tgcgcgccaa cgtcctcgtc cccggcccca tcaattcccc gcaacgcatc
     5461 aaatcccatc cgggcgaagc caaaagcgaa cgcaaaagct acggggacgt gctgcccgca
     5521 tttgtctggt gggcaagtgc cgaaagcaaa gggcggagtg gcgaaatcgt ttacctctaa
     5581 atccggaaaa ggcaggcatc ccgcccgcgc cgcgattccg tttgaaaaat atccgtacct
     5641 tccgcgcgca tccgtcccgc acagacaaac accttgactt aaatttacat aaggacaata
     5701 atgataactc gttatttcct taacttgacc ttagggtaaa ttttatgaaa accgtttcca
     5761 ccgccgttgt ccttgccgcc gctgccgttt cactgcccgg ctgtgcgacc gaatcctcac
     5821 gcagtctcga ggtagcaaaa gtcgcctcct acaatacgca atatcatggt gttcgtaccc
     5881 cgatttccgt cggaacattc gacaaccgct ccagcttcca aaaaggcatt ttctccgacg
     5941 gggaagaccg tttgggcagc caggcaaaaa ccattctggt aacgcacctg caacagacca
     6001 accgcttcaa cgtactgaac cgcaccaatt tgatcgcatt aaaacaggaa tccggcattt
     6061 ccggcaaagc gcataacctg aaaggcgcag attatgtcgt taccggcgat gtaaccgaat
     6121 tcggacgcag agatgtcggc gatcatcagc tcttcggcat tttgggtcgc ggcaaatcgc
     6181 aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt caatacttcc gaaatcgtct
     6241 attccgcaca gggcgcgggc gaatacgcac tttccaaccg tgaaatcatc ggtttcggcg
     6301 gcacttccgg ctacgatgcg actttgaacg gcaaagtttt agacttggca atccgcgaag
     6361 ccgtcaacag cctggttcag gctgttgaca acggcgcatg gcaacccaac cgttaaggag
     6421 aaatgatgaa tccgaaaacc ttgagccgtt tgtcgctgtg tgcggcggtc ttggctctga
     6481 ccgcctgcgg cggcaacggg caaaaatccc tgtattatta cggcggctat cccgataccg
     6541 tctatgaagg tttgaaaaac gacgacactt cgttgggcaa gcagaccgaa aagatggaaa
     6601 aatactttgt ggaagccggc aacaaaaaaa tgaatgccgc cccgggtgcg cacgcccatc
     6661 tgggactgct gctttcccgt tcgggagaca aagagggcgc gttccgccag tttgaagaag
     6721 agaaaaggct gtttcccgaa tcgggcgtat ttatggactt cctgatgaaa acaggtaaag
     6781 gaggcaagcg atgaaacctt tgattttagg gcttgccgcc gtgttggcgc tgtctgcctg
     6841 ccaagttcaa aaagcgcccg atttcgacta cacgtcattc aaggaaagca aaccggcttc
     6901 aattttggtg gttccgccgc tgaacgaatc gcccgatgtc aacggaacat ggggtgtact
     6961 ggcttcgacc gccgcgccgc tttccgaagc cggctattac gtcttccccg ccgcagtcgt
     7021 ggaggaaacc ttcaaacaaa acggcttgac caatgccgcc gatattcacg ccgtccggcc
     7081 ggaaaaactg catcagattt tcggcaatga tgcggttttg tacattacgg ttaccgaata
     7141 cggcacttca tatcaaattt tagacagcgt gacgaccgta tccgccaaag cacggctggt
     7201 cgattcccgc aacggaaaag agttgtggtc gggttcggcc agcatccgcg aaggcagcaa
     7261 caacagcaac agcggcctgt tgggggcttt ggtcagcgca gtggtcaatc agattgccaa
     7321 cagcctgacc gaccgcggtt atcaggtttc caaaaccgcc gcatacaacc tgctgtcgcc
     7381 ctattctcac aacggcatct tgaaaggtcc gagattcgtc gaagagcagc ccaaataagc
     7441 atatgccgtc tgaacctctc cgagcttcag acggcatatg cgaatcttga tacttgcaag
     7501 ccgcaccgcc caaaatcgag cggccggttt caatatccga acaaggttcg ggttctgtat
     7561 tttgcagaac ccgaaccttt tattcaaatt taaacttaat tccggcgtgg ttgtatggta
     7621 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
     7681 aaccgactca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
     7741 caacgacgta ctggtttttg ttaatccact ataaattaac acgcaatgta ccctgaccgg
     7801 caaaacttcc ccaaacttca tcaaaaaatc aactgtaccc gtctttttca gacggcattg
     7861 ataccgtaag aaatttccca tgcagaaatg gcaaaccgag ctttattcca caccgctttg
     7921 gctttgtctg acactgaaaa gcgcggtcaa agtcttgctg ctgattacgg cgatgattac
     7981 gctggtgtta accgaaatgc ggctgaatgt gttgagtacc tttatgtcca aagggcttta
     8041 cgactcgatg caggatttga aatgcttcgg cattttggat gtttgccgca atgaacgcgg
     8101 gcgtggtgct gatacgggcg tttaacaatg tcgtcaacga cttcctcgat caaggcttgg
     8161 cgattaaatg gtcggagcag ctcaatgaag tgctgacgac gcgctggctt gccgacaaaa
     8221 actactaccg cctgcaaatg cgccgccacg cgccggacaa catcgaccaa cgtatccaac
     8281 aggacgcgca ggatttcatc gcttcgacca tcgaatttgt acgcggcatg gtcaattcgg
     8341 tcgttacctc tttggaattt gccgttgttt tgtggggctt ggctggcatc ctgaccgtgt
     8401 tcggcttcga cattccgcgc ggcattgttt ggctcgtctt tatttttgtg attttggcga
     8461 cctttatcgc gatgtggata ggcaacccct tgattcgtta caactatgaa aacgaaaaac
     8521 tcaacggcga ctaccgttat tccctcatcc gcgtacgcga ccacgccgaa agcgtagcgt
     8581 tttacaacgg cgaacaacac gaacacggcc ggctttccga ccgctttaaa gccattatcc
     8641 gcaaccgttg gcgcatcgcg cggcaaagcg tctgcttaag cggctttaac gatatgttca
     8701 ccaacggcat caaactcttc cccattattt tgcaagcgcc gcgcctgttt gccggacaaa
     8761 tcaaaatcgg cgacatccag cagaccgtcc aagcctttgc gcgactgcaa aacgcgctat
     8821 cttttttccg gatgttctac aacaaattca ccgcctaccg cgcccgattg gagcgtctgt
     8881 acggcttttt gctgagtacc gaagaacaac acagcgcgcg gcaacccgag attagcgaag
     8941 tttcagacgg cattgcactg gaatgtaagg cggcggcagc ttttgcacga attggcggca
     9001 gcttttgcac cgccgaagcg taggaaattg tattagcttt tgcacaaatt tatccccgtt
     9061 taatggtctg ttaaacgggg attatcttat gcctttaaaa gtagtacgct acttgcggtc
     9121 agaacttata aatcaggtgg tcgtcgtagc ttgaattgct gttgatgtgc tgctcacagt
     9181 caaggtttgg ctgcctgcaa aacgctccca tccggtcagg cgcagttctg ccacgaggtc
     9241 aagataagta ggcagttcgc tgcgggtgcc ggagaagaag acaaatggcg gccgcaccat
     9301 tgccatcagg cgcagaaact ctaccatgcc gaagtagttc atcgcgtccc cttttccccg
     9361 gcggctcggc tttggttgaa ccgcgcgtcg tgctgcctgc ctttcagttc gctgatatac
     9421 gcctgccggc agtggtggcg gtcgaaaaat acgccgttga tcagggtgta taaaaccttc
     9481 cagcgggttt tgggcttttg ggcgagccgc gcgccgcgat aggtgcggct tgagagggtt
     9541 tcgtccgccg cgccgccggt tagggcgttg aacagttggt cgatggctac caacaggtgg
     9601 taaacgtatt ttttcagccg atgtttagcg tccattcttc gatttccttt tccagcgcgt
     9661 ccaatcctgg cgcggtttcg atggcgttca atttgtcttc gagctgctga cgctttccga
     9721 taatcgcgcc ggcggcaaca gccaggcggg cggatttttc gataactttt tcaatcaaaa
     9781 cgtccaattc cacgcccctt gcggcggcga tttgcgccag catcggggtc ggggcgttgt
     9841 tgtccgcctg ccgcgcgagg gcttcttttt cctgcctgta aaagctgtcg atttccactt
     9901 ggggatagcc cgccaagagg ctgtttttga gttcgtccgc ctttgccgcg aggcggaatg
     9961 ccgtggcggt tttttgttcg gcgaaacggg cggcggcagc ggcttcgccg atttcccatt
    10021 tcttgccgtc ccattcgtgg tattcggacg ggcgcggcgg ggttaaaacg gggcggccgt
    10081 cggaatctgc ggcaatctgc ccgccctgcg cctgtcctgc caaaagggcg gcgtattctt
    10141 cggcgcggac ggcaaccgcg ccttcgggga tgctgcccaa ggtgtcgtcg taaaagccgt
    10201 ttttgaaata gatggtcatt tcggggtttc cttttttgta tttacatttt ggttttttgc
    10261 cgcttataat gtaacgacaa aatacgctgc aagctgcaat gaagattgaa tttgactccg
    10321 aaaaaaatca acgcaatatc gaagagcgga atttgccctt tgaatcagtc gggcaaatac
    10381 gctggacaac ggccgttatt gtgcccgatg tctgctttga ttaccccgaa ccgcgttatg
    10441 ttgccgctgc ttatctaggc gatacgcaac gcctgcacat tgtctgtttt acgccgataa
    10501 aagacggcat ccgtgtcatc agttttcgca aagcgaataa aagagaggtt aaaaaatatg
    10561 caacgtcaat ccttaacaaa cgctgacggc gaagtgcggg aacttgccgc cgaagatttt
    10621 gcgcttgccc gccccattgc cgaagccctg cccgaagact tggcacaggt gcttttttcc
    10681 catcaaaaac aattggaaga aaagggcgta atgcaaaaac gaaacacagg caaaaccccg
    10741 aaacaactcg ttaccatacg cctgtccgcc gatgtggtcg aaaaattccg cgccggcggc
    10801 aagggctggc agacgcgcat caacgaggtt ttgcgccaat atgtcgccca attgaaataa
    10861 cgggggcggc cgctgcttat cgggcgcgcc cgactatcct ttcccgcgcg gtttcggccg
    10921 tgcggttttt tgttgccggt gccgtccgaa gggggcagcg gctttttgcg cggtcattcc
    10981 cgcctttcgt tattcccgcc actttccgtc attcccgcga aggcgggaat cccgaaaccc
    11041 ctgccgccgt cattcccgcg caggcgggaa tccagtccgt tcggtttcag ccgtttccga
    11101 tagattcctg ccgcgtttgg ggtctggatt cccgcctgcg cgggaatgac ggcggcgggg
    11161 gtttctgttt tttccgatag attcctgttg cgtcggggtc tggattcccg ctttcgcggg
    11221 aatgacggcg gcgggggttt ctgttttttc cgatagattc ctgccgcgtt tggggtctag
    11281 attcccgctt tcgcgggaat gacggtggcg ggggtttctg ttttttccga tagattcctg
    11341 ccgcgtttag gggctggatt cccgctttcg cgggaatgac gaaagcggcg ggaatgacga
    11401 agagtggcag gattggcggg gcataggttt cgggggattg gcggcgcggc cggtttcggt
    11461 cggattgctt ggatcggcgg gctgaagccc atctctacag cccatctcta cagcccgtct
    11521 ctacagccat ctctacagcc cgctagtctt gcagtgcctc gagcaagccg tctcggcggc
    11581 ggcggtttcg gggtccggtt gatgttcttc cactctctct ttgcggttct tcgttggttt
    11641 caggtatttc ggggcctatc ggggagctgg cggcgtttcc cgaggcggct ttgccgatgg
    11701 ccatccagtc gcaactgcct tcgtaccagt tggtaataat ggcggcggcg tggttttttg
    11761 cgcggataaa cagccagttg tgttgccttc gatcgccggt aacgcgtccc gaatggcgtt
    11821 cggatacgaa gcatttgacg ttgccgtcgg caaaggcgac ggggaagacg acttcgtttt
    11881 cgatggggcc gccgcttctt gtaaacctgt aactgccggt ctgtatcatt gtgccgtcgg
    11941 ggaatctgac aatatccacg ccgccgatgg tttggcgtgt gaattgcgcg gcaacggcgg
    12001 tgctgatttt ttcgtccagc ccttggattt gggcggcggt gtgggtgtgg tcgcggtcgg
    12061 ctttgtcttg caaaccttgc gccagcgtgc ccgcgtccag cgcgcctgcg ttgatggttt
    12121 cgccgtggga ctttatccaa aacacggctt cgcccaaggt gtcggcggct ttgatgcaca
    12181 gttttaaaac cagggctttg gggcggtttt cgtcgccgcc cgttgccatt ttgctgtccg
    12241 agcgcggggt taaaaagccg ttgtcgttta aatcgccgtc cgtccaagtc gatacgagcg
    12301 cgcttctctg cctttcgttg tggtcttcgt aaccgacggc tgccgtgtct gtgtggtttg
    12361 tccagtgtga aaatactttg tggacgtgcc gtttgatttc gtcttcctgc ttcgttccga
    12421 ctgccaagct gttgcctgcg ttgcggataa agcggtcttc cgcctgcggg acgttttgga
    12481 tgctgccgta ttttccggtc agcagacggt acagctcggg ataagcggtt tcggttacgc
    12541 gcgtgcggat gtcgtcaaac gccagccagc cggtcgggat ttggtcggac ggaaaccacg
    12601 cggtgatgcc gatgtcggta cggctcaaat cgggcaggcg gttgctgttg cccaaggcgc
    12661 ggtagaggtc ggggaaggtg ttttgcgcaa aggtcgtgcc gttggctttg agatagcctg
    12721 cggggttttg tacggctttg ggaaaggata cgatcgcgcc ggcggggatg ccgtccgaat
    12781 tgagtttttt ccatgcggac cagttgtcgt ttgcgctgct cgtttggtgg cgttcgtaca
    12841 cgtcggacgt gtaggcggga taacccaact gcctgcacca gccgggttgc gttccggcga
    12901 tgacttggat atggcaggcg gtgttttcaa cgggcaggtt ggagctgccg accgccgtcg
    12961 gcagggaata gatgccgtct gttttgaggg tgttcaaatc gccccggaat gtttcgactt
    13021 tgaaattgcc gatgccgtat ccggacagtg tgttcggttt gcctttgatg tcttcggcaa
    13081 aacgcggttt gttggctttg tggaagatgt cgtggttgtt gtaggaaatg cggttgtttg
    13141 cgccgtgcag gcggagggtg ttgtcgccga agacgatgtg ggcgttgtcg gcagccgcgc
    13201 cgatgtaggc tttgtggccg ccgttccagt tcgtctggtt ggcggataaa tggatgccgc
    13261 tttggaattg ggtttgggca gtaaaggttt tttggccggt gatggtttgg tcgccggtgg
    13321 taactacagc cagcctattt agtgctgcca gtaattgatt aggctgcgac ttatccggct
    13381 ggataccggc cgcagtcagc acggccagca gctcggattg cacttggttg agccaccacg
    13441 cgggcaggat ggtgcccaat tcggacacgc cgtcgccgtc gtggaaggtc ttgtcggggg
    13501 tttcgatggg gtgcatggtt tagtccttat cggtaggtaa atcggatagc ggtgtgggcg
    13561 ggcttgaggc ggttgaacag gctctcgatc acggcatcgc tgtaatcggt cagcctgtcg
    13621 cccgccgccg agataccggc gcggaatcgg gtaatgcggt tgttgccgcc gcgcacgtta
    13681 acgtgccaca cccacatgat ttcctgcggc gcaagacggt cgccggcgcg gtttacaccg
    13741 gcgcggaacg gctgcggttc gtcgatttgg atttgatagc ccgccgcctc ggccaaacgc
    13801 acaaaataag gaatactcaa gccgcctgtt tcgtttagct tggccatgac ggccaacaca
    13861 cggcgctggc ggtttttgcc cgtaccgtcc aaacctaata cgcgctccca atcggccagc
    13921 atttggccgg cgctgctcgg gtcgacggca tcggcaacgc tttgagccga ttccgccacc
    13981 gcatccagcg ctgcgccgtc tatttctgcc tgcgcccgca cacgcggggc attgcgggca
    14041 tacgacacgg ggggcaacag accccgcaag atgtcttgat agctcatgac ggcatctccg
    14101 ttacattaat cgcgcccgct ttaaaccact cgatgcggtt aaccgtatcg gcggcacggt
    14161 tggccgtcgg cgcagtcaga cggcggtcga tcacaccatc cacattgctg atagccgcct
    14221 cgatttgcga cacagtcaga ccgtcgccgg ggatcagggt gtcgaaatat tcttttagag
    14281 ccgtccgtat gcggcgcttg gcatcgtcca agtcgatgcc gtcgagcttg acttgcacgg
    14341 taacaggcac cgccgttacg gttggcttga gtaccagcgc attttttgcc gttaccgggc
    14401 gcatctcgtc gatataagcc tgtacgcggc gcacagtttc ttccgacggc acaccgtcgg
    14461 cggaggtaat ggcaatatcc accgtaccca agccgcggcg cagcggataa acatatgcgc
    14521 tggttacgcc gtcaacactc aacgcccagt ttttatagtc gtaacggttg ccgcctgcgg
    14581 gcggtcggcg gatgatttcc aacagacgcg ccagcagtga ggcatcgctt tctcggtcgg
    14641 tgccgccttg tacggtaagg cggcattcgg tggacacacc ggcgggggcg gccatcagtt
    14701 gcgcctcgcc gtcgcgcaca ttggcggccg cgcccagctc gtcggcgatt gccggtattt
    14761 ccgccgtgcc gccgctgccg ataacggcgc gggcggtagt gcggtaaaaa cgctggccga
    14821 tacgcacttg caggccgtct gaaagcatgg attgcgcaat accgcttacg gtcagcgtgc
    14881 cgctggccgt ggtaggattg cggcggcgca agccgcgcat ggaggcatgc cgctccaaat
    14941 actcgcggtc ggcggtatca gggaaaatct gccgcacaat ccagctttga tgcgcatatt
    15001 gcccttcggc gcagctggcc aaacggctgg cgtgcacata atggtcgctg tcggggctga
    15061 tatcggcatc cggccacagg cttttggtat cgcgcaggat acgctcgcgg atttgctcaa
    15121 atgtcggcgt ttcaaacacg tttaaaacct ctttaaatca cgggcacttc gtggcggtaa
    15181 tcaaacccgc ccgccgccgt atccacccgg atatgcagga tcaggcggcc gtcgtgcggc
    15241 tgctctgcgc gcacggtaat cttgtcggca cgcccgctct taacaatcgg ttgcagtgcc
    15301 tcatcggcat attgctcggc caacaggctg acccgttgca ggtctttttc gcgctgcaac
    15361 aaatgcagca gcgagccgag cgttttatcc gcccaccagc tgcccaacgg tgtcatcaaa
    15421 cggatataca cggcattttg cagcgtatcg acggtgcggc cggtatagtc gccggtgccg
    15481 gggtttagct ctttgtccat gttggcattt tgccgttttc agacggcctc acggagtaca
    15541 cgggcttcaa tcccgcacgc acaaaaaagc agcccgaagg ctgccaaagg tctgcttgtc
    15601 tatgccggtt ccgccggtaa ggttttgccg ccgatgctgt cggtatgcgg gtgctggcgc
    15661 aacgatatat tgccggccac cacgtcgccg tcggtgttaa agctgccgcc cgtttggtta
    15721 acatcgccgc taaaggtggc tccgtcgccg ccctcgacgg ccatgccgcc gttgccgttg
    15781 atttggcctt gcgccgtcaa cactgcactg gtctccacca acggagcgtt aaatttggca
    15841 tccgtggccg cattaacctc gtattgtttg cagttaaccc ggtacacgtc gcaatcggcc
    15901 tcaatgattt tgccttgctt aatcacgatt tttgcaccct catgattaaa aatcgccgtc
    15961 tcgccgggct taaggttttt gatgcggtag ctgccgtgct ggctgcacac aatcacaccg
    16021 tgcgaagtat tgccgcccag cggtatcact accgcttcgc tgccgtcggg cggatggctg
    16081 gcaaagccgt attcctgcaa atgttcaagg tcttgcaggg tttcgtcggc caagccgctc
    16141 aactgcacgc gctgtatcgg ctcggacgac accaccagcg tgatttttcc ccgaaaggcc
    16201 gcgcgcaggg tttcgccgat atttttagca gtttgtgccg tttttttcgc caatttactc
    16261 aaactcattc aaacaccgcc gtttctgctt gttttttgcc gccttttttg cccttatggc
    16321 tcacgccttt gcgtttgcct ttgcgcttgc gcgccgcctc ggcctttttg gggtaagcgt
    16381 cgggtgtcca aataccgtcc tctttgagcc gcagctcggt ttgcgtgcca tccatgcggg
    16441 atagcataaa ccgccgcccc atcagaaaaa acaccgcatc gataccgtgc tcgtcgtcga
    16501 tcacatgcac acgctggcca ggttgccaca atacgccgtc gcgggtttta tggccgccca
    16561 cggttatcgt gagtgtaaat ccctccagcc gccagtcggc cagctgcttt ttagcctgct
    16621 tttgcaatgc ggccaaattg tcggcatcgg acaccaccac cgttttaggg cggtgcagcg
    16681 tcatcgtcgg gtctttgtac acccacttta aatcgtgttt ggcgctgtcg ccgctgcggc
    16741 cgtgcgattg cgccaaaaaa gtaacctcgg aaaagcggtt gtcggtatcc cactcaatgt
    16801 ccatgcgctc gatattgcag cggctgtcgg tgcggctcca acacaatgtc gccaccggcg
    16861 ggctgctgta atccgcaccg cccaccacca acgtgccgtc cggctccagc cacggatgca
    16921 gcccgaccga gttggcaata tgggttaatg cctgccatac ggtttcgccc ggctcgatgt
    16981 cgattttgtc caaagcgggg ttgttttcgg ccttaagcac caccgcttta atctgcggcc
    17041 acggcgcggc cagctttttg gctgcatcca ataccgtcat gccctttaca ttgagctgcg
    17101 gcgcggagca atccaccaaa aaaccggcca aatcacgccc gctcaagctc aactcgcggc
    17161 tgcccttgct tttgccgtgg cgctggctgc cgatgatgcc cgtcatcacg atttgcccgt
    17221 ctatcactac ctcgcagctc tctccgctta aatcgggtat ggccgcctcc ggccccaacc
    17281 tgccgatgac aaaatcgaag ctgtcggcag ggattaaaaa gtcgctgtcg atgtcgtagc
    17341 gctcccagtg gcggtgctct ttaccgccca cgcgcaccga cacggcatag ccgtatgaat
    17401 tattttgcat agctgttgac caaagtgccg cgcttgataa acgcggggtg gtggatatgg
    17461 ggattgagcc gcaccagctc tgctgcgcgg gccatatcgc cgtaaaactc gtgggcgatt
    17521 tggtgtatcg taccgtcgat tggggcttgg cgcacaatca gcggcggctt ttggttgatg
    17581 gccgccgcaa ccaacgcatt cagacggcct gccgccgcgc gcagggattc tgccgtttgg
    17641 taagcctcgg tatacacggc gttggccgtc agcccaccag actcggcagc agccgtctgc
    17701 accgcccgca aggcggcaat ctcggcctgc atacggcggc gcatggcgcg gttaacctca
    17761 atcaaatcgg gcgcggtcat ctcttcgcca tgcgcctcga ttaatgccgt tgccaccgac
    17821 aacagcgatg acgtggataa caggcgcacc gcctgcgcta ccggctgcac ctgtttggcg
    17881 gttaaccggt tcaggcggtt ttgcaggccg tctgaaaagc ggccggtcag taaattatca
    17941 gggatagcgg cggcacggtc ggcaacagcc acagccccgt caaaccgctg tcgcggcgac
    18001 caaccggcat ggtgcatggc attatcggcc aaacccgcct cacggcgtat gccagtatct
    18061 accatgaccg atatatccgc aaacagcttg gccgagccgt ttttaaacgc cgctgcgctg
    18121 tatccgcccc gatcgggaaa ggcaattttg tccaaatcaa acaaacggcg caccgcacca
    18181 aacgtacccg atgccgcact ccaaatgccc agcgcgctgc cccataaagc cgatacgccc
    18241 gcatccaccg ccaacaccgc atcaacaaag ccgatagccg cctcgcggta ggtgtcgata
    18301 tcagcaatca acgcctcaag ctcgaccaaa aaggcgtttt caaaaacaaa gatttcctgc
    18361 gcttcggccg cctcgcggaa agtaatatcg atgcccgcat aatccacata atcggcctca
    18421 tgtcggtaac tccatgatgc cgcaatcatg ttgtgcatcc gcccccaaac agggtgcacc
    18481 agcacgccgc cgcccggctg ctccagcgca tccagcagct ttttcagacg gcctgcatag
    18541 cccttgcccc aaaacaccgc attaatctgc acctgccgcc cggtcatgcc catgtcttca
    18601 aggtcgatac cctgcacaaa cggccgcgca tgctcggcca atgccttgcc gttgctctcg
    18661 tccaccacct caatatcaaa gccgacgccc ttgtaagatg cgtcctgcaa taaggtatgc
    18721 catccgctca ttattgaccc gctccacggc cgaacatggc cacttgatac cgcgatacct
    18781 cattcgcgat aacacgtccg tcgatattca ggctcacatt ggtattgatg gtctgacttg
    18841 ccgacgccat cgcactttgc acttgtgcca aacctgttgt aactgcagcc gtatcctgcg
    18901 ccaatgctgc ctgatacgcc gccgtctgct gctgaatggc tgcctgatac gccgccgtct
    18961 gagttgaaaa caacggagac aacatcttat cggtaggctg cgctgacgga gcagccaaat
    19021 actgagggac accaccccgc gcctgttccc gcttcggcgc aggttcaaat cggataccgt
    19081 tgtttttcag acgattaatc atttcaggag ttgtcttggt accacgcaaa ccctttggca
    19141 atgtgccgtc acccaaagac tcggaataca gcaacatacc taacaaaccc gcccgtttaa
    19201 ctaacgctgg attattcatg agcccgctac cggcagattt tccccatgac agcaacttac
    19261 cgcctgccgc tgtggcacct gctgccacgc cgcctgcgga agccttaccc cataccaacg
    19321 ctttactacc tacatctttc agaaagcctg cacctttacc gccaccggcg gttaataaca
    19381 ttgcggcaga cgccgctgtt gccgccgtcg tcaatgcttg cagggctaga gtggcattag
    19441 ggtattccgc tgccagcttt ttaaacgagg tttccatatc caccaatgtt cccgtcagac
    19501 tttcccgcaa cattgccagc gattcctgtt gctcttgttg cgccgctgcc gacaacattc
    19561 gcgcctcgtt gttcttagca atttttccgt tatccaacgt tacgccagcc aacgaagcca
    19621 tatattcacg gatttgcgtc atatccgttg cagccagcaa accttgtttt gcctgcaaat
    19681 caggcagcag ttgcgccaaa agcgcgccct taagcatatt tgcctgctcc gccgccgtct
    19741 tatcgcctgc agccgcgcgt ttcttataat cttggtattg cttatccttt actagcatgg
    19801 catcggcaag acgggacaac acctgcactg cgttttcgcc gttttgcttg ccttgcacaa
    19861 ccgagcctat ccaatcgaca cctttcttcg ggtcatttgg atttgccatc ttcttcagac
    19921 gacctatcgt gtcaggcgac agagttttac tcaaaagatt ttgcacatta gtcgccgctt
    19981 cggcaggact gcccgattta ttcgccgcag attgtaagag tgagagcagg tagtccaaac
    20041 cgccgacacc attcatccct gcctgttgcg cggcagggag caggctcggc agctcccgaa
    20101 ccatatcccg cacctcgaaa gtgccgtcta aacccgattg caagacgtgc tcaagcccga
    20161 gttgcaggtc tttaccgctc atgccgccat ctttcagggt tttaatcagt tttgccgtat
    20221 cctcgccact gccttctgag gcaagtgcaa aagcatatgc cgcctgcgct tcattcttgg
    20281 tttgggcaaa attcagactg gtggtcatca tgccgctgat taaatccaaa gccttatcgt
    20341 gggtcccgcc atttttctcg acaagttcaa gtgccaaatc cttgatctgt tgcgcacctt
    20401 cagttgcaat ccacgctgcc gatttactgt tatcctcaat aaatgcctgt ctggacacgc
    20461 ggttgatgtt ctcatcaagc tgctttctgt tgtccatagc aggcttaagc accgtatacg
    20521 ctgccgcgcc accagccacc gcagctgcac cgaatcttcc gatttttccc atcttgtccg
    20581 caaatcccgt gccctgtttc agttccgcgt tcagctcgcg aattcggtta cgcgtagcga
    20641 ccgccgcccg tgccaaatca ttttgtgatg ccttgccgct gcgcgccaaa cggttaaacg
    20701 cagcctgtgt cagcatgatt tcgcgttgaa tctgtttttc tgagcggatg ccggtcaacg
    20761 cataagtatg gctttgccgt tcaagttgcg cccgcgattt cgcggtacga tctaattgtc
    20821 gttgagtatc agccagtagc cgtctcgctc cgtcatctcg ggcggttaac accaacgaca
    20881 gtttcatgtt cccgtttgcc ataaaaagca gcctttaaac gtattaactc aaggtcaatt
    20941 atcgtttaaa ggctgtttaa gggaggcggt atgggcttca accccgtttt cgtgctttat
    21001 ttgtttgttg cgttataata ccagaatgat aaccatttta ttcaaaggga tattttatga
    21061 atatgaaaaa attgatttcc gcaatttgtg tttcaattgt tttatcagcc tgcaaccaac
    21121 aatcaaaaac ggcacaagcc gaagaacctg tccaaagtat ccaggctgct gattgtaccg
    21181 ccccaatgga catcacagtt gaacaatatc tcatcaattt ggagcaagca tttaaaactc
    21241 agaacgtctc aacaaaaatc cataataaaa atattgtcaa gaccgattgt ggttatgacc
    21301 ttactttggt aatggatttt ggggcgattg cgctcaaact ggacgagcag caaaaaatta
    21361 gagctatctc agtaggctac attttaaaaa ccgacggaga gaaaggacaa aatctagtca
    21421 ataatgccat aaatggatta cacagtattc aggcagttct gtctttaact accacagaca
    21481 aattgggcga atcggaagca ggaaaacaac tttttacagc tttaaccgaa gtcgtcaaag
    21541 aatccaatca gacaggagca acagcgcaaa aagacgttcc ggcagatggt attttatata
    21601 gcgttgtttt tgaaaaagaa acaaacacca ttgcaataat cggcagaaaa caaccctaac
    21661 cgtcaaataa aaaaaaggtc gtctgaaact cagacgacct ttttattttc cctccggctt
    21721 tctcagccgt ttcgacacaa tcactccctc ttcctttggc gtttttatgc ccaggctcga
    21781 caggatgtct tcaaaccatg ccgacagctc ggcgtggcac atatcgcgca ctgcgtccgc
    21841 ccctatcccc gcctttgcca tcaggatgac cgcctgacgg tagcttttgt gtgccgtcag
    21901 gtagctgcgt tttactgctc cgccgccgac ggcgcgtcgg ggctcgccgt agcggcggac
    21961 ggttttaggc acaaatcctc ctgagccgcc atcacggtat ggtagtcttc ggtagagagg
    22021 ttttccatca gccactgcgc cgtcaggatg tcggatggga taccttgggc ttcaatctgc
    22081 tgcgaccagt aggtcagcgt ctctaggacg tcgcgttgca gcagttcggc ttttgtggcg
    22141 ttttcgggca gctcgggcaa gtcatcaatc agggcgaacg ccgccagttc gccgccgacg
    22201 gtcagtggct tgaggcgtaa ttcagatacg actttgccat tgtaggtcag cccgtgggtc
    22261 agtttgacgg taatcacgca ttactccttg attttgtgca aagcaatcat ctcaatgtcg
    22321 atgcgtgcct cgttatcgac ttcatattgc tcgctggtat cgacggtaaa gcagtcgagg
    22381 taggaagtgc ggcgaccttc gtcgttgatg gggtaaatcg tgattttcgc cttggtgatg
    22441 ttgtcccagt cgatttccgt accgtcgatc ggaatggcgg cggtaacgct taatttgtgt
    22501 tcggttacgc cgtcacaata accgttgact cggccgttgc ggttcatcgt tttgaccggc
    22561 ttgcggcctg tagtggtttg cggtttgatg ctcacaatct cgatatcgcg gccgttcacc
    22621 tccataatca ccgcgccggt ataggtagcg tcgctcatgc tttatccttt tttaaggttt
    22681 cagacggcct ttaaacctgt tttaaacgcc gtctgaaaag ggttacaaaa tcaaatcaat
    22741 gcgcccggca aagacgtgca ggccgttgac cacatcggcg gggataatgg cattaacacg
    22801 gttggggtcg ttttgcgcac gcgccaccac cagcttgcct ttgttggcct cggcgttttc
    22861 gatgatttcg gcttggtcga gcttaatcag cacgtccaaa atctcgctct taaccttggg
    22921 cagcaggcgg tcgctcaatt tgtcgcgcgg aaaacgcagg gcaatgcgct ctttaacgct
    22981 gcggcgcaca taatccagcg tgcgaatggt ggtaatgtcg agtagtgccg ggtcgtcggt
    23041 gttgttggcc gacttggtat aggttgatac ggcacgcata atctgcacgc ggttgttgac
    23101 cgctgtgagc ggggtcaagc cgttgtacag cgcattgttg cattctgcaa accacggcca
    23161 ttgcgcatca ggtgtaacgg ccagcccttt gatttccagc gtgttcagcg gcttggcagg
    23221 gtcttcttca aaggccaaca ccgccgcata acctgcggcg atgatgccgt ttggctctac
    23281 cgcacctttg taccaagcac aggtaatgcg gccgtcgttg attttgccgg tagcggtggt
    23341 ggccgtgctc aaggccgcac tcatacccaa tacgccgata cagccgcgct gctcgatggc
    23401 gttgaatacg ttggtaatat ggttgctcaa ggctttggcg ttggcgtcat cgctaaacgg
    23461 gctgcaaata atgtgataat gcttgcccgc cactttggac aaggccgtgg caatgtccgc
    23521 atttttggca ccgccggtaa agacattggc ttgataagtc aggccggtat tgccggtgct
    23581 cacggttaag ccgctctcgt tgccgatctc gcctttgtgt ttggccgtca gcgtaacggc
    23641 tgcgccgctg ccggatgcgg taacggttac atcggcggca gtgatggcgg ttttcagacg
    23701 gcctgccact gtggcggcgg tctcgccggt gttaacggcc gtgcttacct gcttgccgcc
    23761 aatcgtgatt tccaccacgc ccggcgcggt ggccgtgccg gaaagggtaa cggttgcggt
    23821 tgcctgcacg cctgcgctgt ggtcggcaat accgataacg gtcaaatcca aataagggtt
    23881 gttggcaaat gcttggcgca ccatcaaatg cgccagcgag ccttgtccga acaaatcggc
    23941 cgcctcggca tcgctaaata gttgcaccgg ctctaaggcg ggctgtatgc ccgcggtcag
    24001 catgggtgca accatcaata ccttttgcgg attttgcggc aaaccttgca cggcattgcg
    24061 ggtgttaaat tcaatatact gcccgggcac gcggatgctg cccggaatcg tgtcaaaatc
    24121 aatatggggc atcattactc tcccttagta ttgcgggttt tggtatttgg ggcggcatcc
    24181 tcaaccacca ccaaatcgcc gtcatcaatc atgcggcggt aatacaggct gttgccgtcc
    24241 acctccaccg gctcttggcc gatatattcg tgcgggttgt gttcggtcgg cacacgcagg
    24301 ccatctgccg ccgttacttt gattttgctc attgcttatc cttaagggtt aggtttacag
    24361 gtatttcatc ggcggattgc gggtcgtaaa ttttgccgtc caacccctcg aaatcaggcc
    24421 acggctcgct caatgtaccc tgatacttgg taaagatatg gttggggtcg tcgggattgt
    24481 cggtgcgttc ggggtagcgg tcattttcca acccgcaggt gttaaagcgg atggcatact
    24541 caaccgcata tatgcttact gcggcgtttt gcaccagcac atgattggca atcgcgcgca
    24601 ccgctttggg caccaagccg cggctatcgg caaaaccgag ccgctggccg tcaagcaggc
    24661 ggcgaacagc gcggattaaa tcgttgctgc cgatttcgcg gctgtcgatg ccgccttgcc
    24721 gctgcgcctg ctcgttgcgc agattgcggg ccgccaccat caccacaaat tcggcggtat
    24781 cctgataacg tccgcatacg ccgccggtgc tggcaggctc aactttgctg ccgccatacg
    24841 ttacccaaac cgcaggcagc gtatggattt gccccgccaa atcgtcggcc tcgccgttgt
    24901 agcttttaac cgtgcgcacc atgcgaccca agccccgttt cagacggtct gttatcgctt
    24961 gctcaatatc agtgatcacg cccgaacacc ttatttttac cgtttacaaa cacaataccg
    25021 tcttcgcccg cggccacttc ttcgccgcta tcatccacgc ccagctgcac ttcgcctttg
    25081 gccatagcac gcagcaggtc gagcacgtcg attttgtagc ggttgcggat ttcgtcggta
    25141 atcaacacgc cctgagccgc cgtcagacgg tagcgggcaa tgtcgcagca aaggcgcacc
    25201 aagatgggcg gcagatcctc aaaaggtcgt ctgaaccgcc ccagatacgc gtcgatttcg
    25261 gcagtggcgt ccaccagcgc ggtttgtgcg acctcgcggt caatcagccc ctcgttgttg
    25321 cggtcggtga gctgcaagac ttccagctca ccgaaacgcg caaccatatc ctcaaccgtc
    25381 gcgtatgcca ttactcgacc gccttgcgtt gcagcatagg ctcggcgcag attgccttcc
    25441 acaccgcttc gccgacttcg gcgcgcttca cttcacgcca gccgccgtca aacagcaggc
    25501 cgccgcgcca aaattctttg ccgtctgcgc cggtactgac gagcatcaca tcgcggctgt
    25561 ccgccaaagc gtcggcggcg cgttgcgtat gctgcacttt gagttcggca agttcggcgg
    25621 acagtgcctt tttgtcgtct tcggcttttt ccaaggctgt ggtcagcatt tcgacatcgt
    25681 ttcgggcggc ggcaagctct gcctgcacgg cgtccaattc ggctttgatg tcttcaaacg
    25741 acggggcggc ggtttcggcg gtttctggtt tgttgttggt ttttgccatg atgactcctt
    25801 gtttcagacg gcctttaaat aggtgttaaa ggtcgtctga aaacggttcg gggttacagg
    25861 tggacgcaaa cgtgcagttt cagacggcct ttgaaggtat tggtcgtgcc gttgattttg
    25921 tcggcttcca ataattcacg ggctttgtct tccagctcgg gcggaacgac caagaggctg
    25981 ggcttcacat tcaacacata gccaccgtcg gctttgattt tctgcatttt gacgataatg
    26041 gcggcgaggt tttcgcgggt cagcgcggtt ttttccgcca tgtgcatcag ttgccacaaa
    26101 ccgaaaccgg cattgcagcg gcggcgcgat ccgtacaggt attcgtcttc cataaacacg
    26161 cggtcggatt tggacgggtc gaatttggtt tcaaactcgg agtcggtgcg gttttggaaa
    26221 atcagcggct tcagggtctt ggtgtcatcg acgacgtaga aggtcggcgc gtcgttgtcc
    26281 gtaccggtcg tcagattgga gtgcggcgta ttctgacccg tgccgtccga tttctcgtaa
    26341 acggggtggt cggtatcgaa gaaatattgg ccgtcataac cgacagtggt tttacctttt
    26401 ttgagcagac cccacaccag cgtatcgggc agggcggcgg cggactcgcc cattgccgcc
    26461 atcatcgggc ggtacatgcc gacttggtcg tcttcgatgt ccgtacgcgc tacgccgacg
    26521 gttgcttcaa actttttgtt gtcgaggctc atcgcctgct tgttcatttt ttcgatttgg
    26581 cgcgagccga cccattcgcg catttggggg aatttgccga gccacgcata agtattggtt
    26641 gccgtattgg acggaatggt catcgcaacg gcggtatagt cgggcttgac ggattccaaa
    26701 cccgtttgaa attctttgcg gaatgctgcc gtgattgcgg tcaaaatcgc tgatttgtcc
    26761 attacttacc ttcgctttct ttgattttta caaattcttc gccggacatg cccagcattt
    26821 ttgctgcggc tgcctcttct gcagtcagtg cggcgacgcg ttcgtcgggt gctttaccgc
    26881 ccgtttgcga gcctgccagt gcagcgaccg gctgggcgtt ttcaataaag ccggtcaaaa
    26941 atgccaagcc gcccggctgt ttcaatacgc cttctgccca ctccttctga gcaggcagca
    27001 atttgcctga agtcagcgcg gcggtaatca attcgttgcc tttgtctgct tcctgcttgg
    27061 cagtcagcgc ggcgactttg ctttgcagct cttgaaccac tgagataggc gcgtatttag
    27121 tcaaatcagg cttggcggtt tgcgccgtca atgccgcgat gcggctgtct ttttccgcca
    27181 gctgcgcgaa tacatcggcg gacaaagccg caccttccgg cttggcttcc acgagcgcgg
    27241 acaatgccgc cttcagttct tcttcgcccg catcaggcag gccgaacagt tgctgtaaca
    27301 actctttcat agggttttgc tccgtttccg gttttaaaat ttgcgccgac gctgccgcca
    27361 gcacttcgtc cataccgtcc aacgcgggga aatttgtcag cgcggcgtga aaaattttgc
    27421 ttacatatcc ctttgtgtca taggaaaaca cagcagagat gtagcgatac tcttttgcgg
    27481 caattgccgc agccgccttg tccgtccact ccacttcggc aaacatgcct ttaggcgtga
    27541 actccagcca acgcatccaa ccggcggcag gtgcaggttg tccgtttttc tctttgtaga
    27601 gcgtctggtg ttcataatcg acaaccaact gattgcgcga gctgttggcc aacaacgcga
    27661 catcatgacc gttttcttcg gtcagatacc acgcagggac atcagtcgga cgaccgtcta
    27721 ctgcgcgaaa ttcgccatat ggcagcagtt ggattcgccc gtcttttggc tgcacctcga
    27781 aactgcacac ggcaagtagg gttttttgtg catttttgga catgtgtacc tctctcaata
    27841 cggtcatttt gcctatttga aaaaacaaca aagagtaggc gtgcttcaaa ccctcccgcc
    27901 atttttttaa aacctgtccg cgcgatacac aattaatcgt tttcaggggc tttttagaag
    27961 cgtctaggat tgatttttat atcttgggca ggggtttgta ttacccgcat aaaaaaacgc
    28021 gctaaaaagc gcgtttttga gggttttata aattcacggt atcagacccg aaaaataatc
    28081 ctgcacatcg tccatcaaag cctgtttgtc gtcgtccgtc agcgtcaaaa attcccgttg
    28141 cggaatccga actttgcggt tgcgccccgc cataccgccg aagttgtgga tggcggcata
    28201 gacgatattc gtaccgacaa gggctgtatc gttgtctgac agtgtggaaa aactgtcttt
    28261 cagacgaccc gaatccgaaa gcggcttgcc gtcgcggtat tttagcccca accatttcgg
    28321 acggcctgcg tagcggaaat tgagcttgac cgccgtgtgc atggtttcgg acagtcggcg
    28381 catcagcagg tagcggtttt cgatcccgtt gccaagccgc tcgatttggt ttaggacgac
    28441 aaagatattg tctattttga catcaatcat ggtattatcc tgcctaggct actaaaaaag
    28501 cggtgaattt gtctgccctc cgcagccctt atgcagtcct cggactggga ttggtatccg
    28561 ttacataagc gagagtgtgt cgggattgca gtccgactta gtagcctttt ttcatttcat
    28621 agcgtttctt acttttcaca atttcttttt catccgccct gcgaaacgaa tccaaaaaaa
    28681 tcgaatcgta ctgtggcaga tatttcaaaa ctgccatcaa tctgtatttt tcgttcccct
    28741 ccaacggttt tccgagcaaa acaaaacttt cttttcctct gcttttgtca taaaaaatat
    28801 attcgggctc atgaatcaga tcggggagca tggcatataa atctgttcca aacgattgcc
    28861 cctcccgact gtccacctgt ttgaccaatg tatcatcaga cagccacacc gtcgctcgtg
    28921 tcatacctgc caattcttgc gtttccttgc tcaatacacc cgcagcaaat ttaagctgtc
    28981 ttgatagcgc atttcggatt ttgattttct gctctttatc gggcttgcca tcaatatcca
    29041 aacgttgctt gacttcataa aactcttttt caagctgttt aaagctggtt ttaaaatccg
    29101 caccacccat ttccgctttg gcaaattgat gcgccaacgc acggtcgtac ttgtctaaat
    29161 cggggcggta gttcattcgt ccggcgttgt aatcaaatcc tcgatcggtc gtgtacagac
    29221 tgccatccgg tgctttatag gcaagggtca gataagtatc gccttttttg ttgtagattt
    29281 tatgggtctc gactagattg tccgccgtgc tttgcccgac aatccgcccc tggcgttcca
    29341 catcccgctc cgacagcgcg atgaccgagc aacggcagtt gtagccgttg ggcgggtaaa
    29401 acgttgccca aaacgggtcg tcgtagcggt acaccagccc gtctattgcc gaatgcgccg
    29461 gacgggtgcg gctgtcgcct accgcgtcat acatccaata aggccgtgca tcaatatttg
    29521 ccatatatcc ttgatattga ccggcgttgt aggcagtttg catattggta cggtaaatcg
    29581 tctccaacct ccgcggcgaa ccgaatacct cgccggtggc tgggtcgatg atatccttac
    29641 cgttatgccc gttcggatgc agccagccct tgttgccgag cagattcaag ataccttttc
    29701 gccaatcgtc aaacgactgt ccactttttg ccgattcaac catcgaagtt ttgatgtcgt
    29761 tgagcatatc caagtcggtc atgcgggcaa tcgtatagac tttggcaatt tcggaggctg
    29821 tcagattgcg gtagctctcc gccgtaacct ttttactttc cagccactcg attgcctttt
    29881 ttggcggcag acttaaggca aatcccaaat caggcgcggg catggtcttg tcccaaaata
    29941 tcgctgataa acaaggcctg ctgcatatag gtacgcagtt tcgcgttgtc caaattcgga
    30001 taaagcgcat tcagtgcggc atctgcctcc tcgtagctgt tgcaagcatt aagtgcggca
    30061 accgcctgac gcaccatcgg gttgagctga gaattgaaat cgggctcaac cagcgcgtca
    30121 tccaacgcgc cgtccaatat ttcctgatgc ctgcccgtag ccttagatgg tacggtgtgg
    30181 gcggataaag ccgccaatgc agttctgttt atcggattgt ccggtacctg ccgcaccaac
    30241 acagcctcac cctcctgcac atctggaatg accagtttgt cgcgcaccca gctttcgggg
    30301 atttgtacgc cgacatccac cagtttcggg atagcgtcgg caaagaccgc gatgtctttc
    30361 ggctcgcgcg tgtcaaattc aaatttcggc acgcggtttg ggtcggcatt gggatagttg
    30421 atttgcagga acggtccgat gatttggctt gtgatggttt gcgccacctg ttttgcgtcc
    30481 gacaccagca aatcgcggcg tacctcgttg tggatattgc ccagcgcgtt ggtgctggat
    30541 tttccgtccg caccgctggt tagcgtttgc cccaaaatca gccgcgccgc cgatttttcg
    30601 caccagtcgg ccatctgcaa aaacggattg ctggttgcca tcgtaccgct tgcagcgttg
    30661 tgcagctcga tttccatacc ttctggcatg atgcctgccg cgttgtgacc gatttccgcc
    30721 accgctcgaa gcagggtgtt tttttcctct ttggttgcgc ccgcgccgta tttgccgata
    30781 cggatgggca tgccgtacag ctccaaaaac tcggcaaaat cgtggacggc gtagtgtttg
    30841 aacatataca gccaggaaag cgtgcggaaa agcccgttgc gcgcctgctg gacgctgcgc
    30901 gatttttggg tatgaacgac ccagcccagc ggccacaacg cttcgccttc cggattttcg
    30961 cgggtacgca gcagcagccc gttgtctttg tcccatttga accagctttg cgggcggtgg
    31021 ataaagtttc ggggtaggta aaggccgtct gaaaataccc actcgacctc caacgcagaa
    31081 aatccgtgcc ctaccgcgtc catcaaatcc ataatcaggt cttcgagggt aggcaggctg
    31141 tccatcattt cgtaggcttg gtcggacagc ttttcttctt cgggcgtcgc atttcgcggc
    31201 ggggcgacgc gccagttgag cgtcagcagc gcgcgtttgc gcgtccccat atttgccgcg
    31261 atgtcgctgt cgcgctcctc aatgtccgcg aaaagctcgt gttgggcgcg gatgtcgccg
    31321 ctttccgcat cctcgaagag ggcccgcatc ttttgcggtg taataaaatt ggacggatgc
    31381 tcggcgataa cccgaccggt cgccgtaatt tgagccacgt ccgtctgcaa tgcagcttcg
    31441 ggcttttgga ttttagtttt attgttcttt tttgccatta tcggctccat ttacttttta
    31501 tctcgtcatc aaaatcgtta tcggtagggc tttgccattc tatcggcaca caattggcca
    31561 ccgcccccga ccagagcata tgcactgcat caggaccatc atcatgatca gcctttggaa
    31621 aatggcggaa ctgctgtatc agcgtctgtt ggctctcatt caacagaatc aaaccgttcg
    31681 ccatgtgagg ctgtaaagtc tcaatccgca agagcttgtc cgataccggc ttgaccgccc
    31741 gcgccgggac aggtattcca cgcgccgcgc tgcgcttgac cagctcatct ttcaaaaatt
    31801 cctgaaactg aaccgtctca acaaaccaca gtttgcaacg atattgacgg tgcaatctga
    31861 taacgtcctc aataatcaaa tcagggagac gttttttaat ctgagcttcc acgacataca
    31921 gtttgccggt ttcacgttga taaccgccaa tgataatcgc cgacgggtca cggctcgccc
    31981 ccgctttacc cagcgacggg tcgagcgcac cgaaatacac caaatcggac ggcaggtcgt
    32041 tccaaaactt catcgacttg gcaaacggcg catcttcgcc gctgaccggg tcgttttgat
    32101 attcgctgtc aaatgtcgca tgaccgtcac gcgcacggat tttcatcagc gcgagtacgc
    32161 cgcgagccgc ccagcttgtt tgcgcaccgc gctccatctc gtctttattg gcgagataaa
    32221 acgcctcggc cacagtctcg ccgtcgtttc ggaaaagttc ctcccatctg tcccacaaat
    32281 ccatgcggtc gggccagcgt ttcatcgcct taaacttaat accgtgccaa aacgggttat
    32341 tcaaagtgcg gttaagtacg ctgtcgtaat gcaaaatcgt gccgatatat atcacatcgt
    32401 atttctggcc gacaccgccc aaaggcaata cagtcttagt cagccacgca ttgagcttgt
    32461 cgcgctgttc ggggttgcgg acttgctcgt cattctcaat atcgtccaat atggtcaggt
    32521 caggacggta agggccgtga cgcaaaccgc gcagcttttt accgctaccg gccacttgga
    32581 ctttaacgtc attggccgtc acaatcgtac cggcctgcca tacgcggccc tgcccgcata
    32641 cttccggaaa gtcggttttc aagcgcggat taaattccaa ttccgccttg atggcttcga
    32701 gcatcgggta tgcctgatct atgctgtcca tcacaataac ggcataatgt ttttggcctg
    32761 tcacaataca ccacagcgta aacaactgag taacctgcgt cgacttaccc tcgccacgcg
    32821 gcgcacccac cgcctcattt tcccctttgg gggagcggat aatctccggc agacggctga
    32881 ataaaaacgc atgcagttcg gatttttcag gggagcggat ataatgcgga aagtaggtgt
    32941 tgacgaaata ctcgtaaccg cctaccgggt caaacacctt ggcacggcgt gcagcaatag
    33001 ccttgggcga cgcatcaaag ccgtccactt ccgcttcgat gacttggcgc aaactggcgg
    33061 ccagttcggc aagggatttg aggaattctt ttgttttcat gagtatttat aatgactgga
    33121 ttcatttcta acgcatttat aggcctaaaa gaagcaggat cacttttaaa agggtttaac
    33181 cagctcaaaa cagaggcaga ggtagcgcaa aaaaccatcg aacttaatca aatcattgct
    33241 ggtattcaaa ctgatttatt tgaagcccaa tcttcttata cggcggcaat tagccgtata
    33301 gatgagttgg aaaaagaact ggtggcagta aaaaattggt ctacagagaa gcaacgctac
    33361 caattgtatg aacttgctcc tggttctttc gtctatcgat tgaaaacaga gatggcgaac
    33421 agcgagccaa ttcatgatat ttgtccccaa tgttacgagc agggtgtcaa atcgattctt
    33481 caattcaatg ggtatgaagg tgcttttcat aaatattcct gttcccgttg ccaaaccata
    33541 atattgggag agcgtttaaa gtcagatgtt gaagtatttt cagtaggagg atcacgttat
    33601 gggagattga tagacggcta ttaaccaaac ttcttctcca cttccgcccc aaacggctcc
    33661 aatacctcca caaaggcagg caaatgtttg ggatgttttt cttgcacaaa cgccatcaag
    33721 aactcaatca attccaaagc cgtcgccagt tttgacgttt ccggcatcac acgcgcattg
    33781 gccgatacgg ttttcgtaaa cgcatcggcc aggctggcca acagcttggc gcggtcggac
    33841 gggggcaaat cttcggtact cgaatcctgc agcatcgtca tcgtgctgtt gtactgcacc
    33901 ataaaaccgg ccaacatcgc acggctcaag tcctcaatac cgccgccggc caaagtgtag
    33961 gcggcgcgca tcttatccca gtcgtcgcct ttttccttat ccgcacgttt ccacgcacgc
    34021 gcagtggtct gcgggatttc gcacatcaag gccgccgttt ccaaagtttg ctcgccgctc
    34081 acatagagcc gacgtaactt ttcacggatt tcttgcgggt gagccataat tacagtccca
    34141 ttttcgcttt aagcaattcc cagccgaccg taatcacgcc gccgcccagt gcgccgaatg
    34201 taatggccgt gcgtttcgtg tcttggcgga tttgcgcaat ttccgcctgc atttctttct
    34261 gatttttcag cgtttgatca gtcttgtttt caatacgcgc caaggcttcc aaaatcgggg
    34321 cgctcatgat ttgtctgctt tcctgtccag tttttcgttt actttttcca acttgttttc
    34381 gatgcgtccc aacgaagccg caatattgtc gcggtctgcc ttggcatctt gcttggtgtg
    34441 ataagagagc ttgaccgcgt gcagctcctc tttcaggtcg tcgatacgct tgtccgcctc
    34501 tttcagacgg cctgaaatgc cgttgaccca aaaccaaaat gccgccgtcg caatcggcca
    34561 cagggttctg aaaccaaatt caaagtccat ttaaaacccc tttaaaccgg cacatcgccg
    34621 aatacgatac ggacggcgta accgtcaggg cgattgctgg caatctccag tccatcacca
    34681 tcattacaaa cacagtaata cgcccccaaa gtttgccaaa ccgcacgttt aaaggtgtca
    34741 tagttcgtaa ttggatattc aaggtgaaac gtcgtctgaa aatccttatc catgcgtacc
    34801 gcgtactcaa tgcctgcctt atccaacaga tcggaaacat gaatgacaaa cggctcttgc
    34861 tcgcgtgcgc ggcttaagcc caattccaaa tcggcatggc gcacagccaa ctgacgttca
    34921 actaattcac ggtaggtcat tctttgatac ccattaaata ttttgtccgc ttgtacaatt
    34981 tcttaaccca cgaaatattt acaaatgtat aaacctttgc tacaacttcg ccgtcatagt
    35041 gcgcattttc tcgtgcagcc cgaaatttcg ccctggcttc ttcagggttg tccgcccaaa
    35101 tgctcaatga ccaggacttg ccgttaaagc ggtaagaaaa cgtgtactca ttcataggag
    35161 aaaccttatg tattttgaaa tctataaaga cgcaaaaggc gaataccgtt ggcgtttgaa
    35221 agcagccaac catgaaatca tcgctcaggg cgaaggctac accagcaagc aaaactgtca
    35281 gcacgcagtc gatttgctga aaagcactac cgccgctacc cctgtaaaag aggtataaaa
    35341 tccgctttca ccctcagccc gcgccctacg cgggcttttt tgtcagtttc cgactttgcg
    35401 ggagtgattg cccgcccagt cgcgccaagc ctgattttga ttttccaact ccgccacata
    35461 gccgccaaac tcggcggcgt gttccaacag cgtggccgtc ttgccgtctt tcggcggatt
    35521 tgggcgtacc ggcgcgacca tcaatgcggc aggcggtgtc ggcatgaccg ccttttcgac
    35581 aaccttaatt tccgtagccg agggcgcgct tgtagagctg caagccgtga tggccaaagc
    35641 cgtcaataca accgtcgcct gcatttttac ggtcttgagt aaggacattt tcgatttcct
    35701 ttttattttc cgttttcaga cggctgactt ccgcctgttt tttcgccaaa gccatgccga
    35761 cggcgtgcgc cttgacttca tattttttcg cttccgcgcg cgcctgttcc agttcgcggg
    35821 cgtagttttg agccgacaac agcagggctt gcgctttgtc tttttcctgc ctgtcgctgg
    35881 gtttttaaaa atatccaaaa tacgcattgc tattccacac ttttggtttg caggtgccgt
    35941 ttcagcattt cctgataatt ggccagttcg ccctccgcaa attcaaacgc cgccaagtct
    36001 gcctgttcgc ttgcctcacg gcttttggcc gaccacagcc caatcatctt ttcgtaaaac
    36061 gcaacctgtc ccatgattaa cgacgattct tgcgtttacg cgccgcccgt tttgcggctg
    36121 ccacgcctga gtttcttaaa tttgggcgtg gataggtgtg tatcggacga acatttggca
    36181 tctgtataag tgcgcggttt acctgctcat tactaggatt ttctccaatc aattttgtaa
    36241 gaaaatagga agcagcttct ttcaatttac caataatatt catactcttg ccgcccccaa
    36301 tttcatcgca atcgcgtccg caatcgcgcg gcagatgccc catttggtcg tcttaaacaa
    36361 ggccaaatca gtgtcgttgc tgatgaaaaa aggctcaaca atgccgccag cctgcgcata
    36421 agccaggcgc gaatgatggc ccgcattgtc gggtttaaag ccgtcttcgc cgcgcagttt
    36481 ccagccggtt ttcttggcaa cagctttgct caacacctga caccagcgtt tgtttttaac
    36541 ggtactcaac gcctcaatgc ccgtagctgc tttaccggcc gccgagttgg tatggaactc
    36601 aatcgccaca tccgagccgc gaatcagttt tacagcttca cgcagcggca tattgccttt
    36661 gcctgtgccg tcggttttaa cagtcaaacc gtagtcatcg cgcaaaatag cagccacgat
    36721 gttgcgcata tcctgcgcca agtccgcctc acggtcgctg ccgttgaccg cacccgggtc
    36781 ggtgttgctg tgtccagcgg ttaaggttac ggttttgccc attcatcatc tccaaaaaat
    36841 attgatt
//
LOCUS       DAWKQJ010000004        35153 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000004 DAWKQJ010000000
VERSION     DAWKQJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.004"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(144..234)
                     /locus_tag="WJL20_000140"
     tRNA            complement(144..234)
                     /locus_tag="WJL20_000140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(270..356)
                     /locus_tag="WJL20_000141"
     tRNA            complement(270..356)
                     /locus_tag="WJL20_000141"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Leu,seq:cag)
     gene            complement(392..478)
                     /locus_tag="WJL20_000142"
     tRNA            complement(392..478)
                     /locus_tag="WJL20_000142"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Leu,seq:cag)
     gene            567..2942
                     /gene="rnr"
                     /locus_tag="WJL20_000143"
     CDS             567..2942
                     /gene="rnr"
                     /locus_tag="WJL20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ7682354.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKSDLVKCRIEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFGEACAKAAKKIPNHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPHVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIEHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3281..4744)
                     /gene="guaB"
                     /locus_tag="WJL20_000144"
     CDS             complement(3281..4744)
                     /gene="guaB"
                     /locus_tag="WJL20_000144"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ7682355.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4891..7449)
                     /gene="glnD"
                     /locus_tag="WJL20_000145"
     CDS             complement(4891..7449)
                     /gene="glnD"
                     /locus_tag="WJL20_000145"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ7682356.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKAKRVSVFFREYTAAVEILL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPSPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(7498..7818)
                     /locus_tag="WJL20_000146"
     CDS             complement(7498..7818)
                     /locus_tag="WJL20_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7682357.1"
                     /translation="MMGNKLTPPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8143..8616)
                     /gene="bfr"
                     /locus_tag="WJL20_000147"
     CDS             complement(8143..8616)
                     /gene="bfr"
                     /locus_tag="WJL20_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7682358.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8644..9108)
                     /gene="bfr"
                     /locus_tag="WJL20_000148"
     CDS             complement(8644..9108)
                     /gene="bfr"
                     /locus_tag="WJL20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7682359.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9571..10554)
                     /gene="lipA"
                     /locus_tag="WJL20_000149"
     CDS             complement(9571..10554)
                     /gene="lipA"
                     /locus_tag="WJL20_000149"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ7682360.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10547..11170)
                     /gene="lipB"
                     /locus_tag="WJL20_000150"
     CDS             complement(10547..11170)
                     /gene="lipB"
                     /locus_tag="WJL20_000150"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ7682361.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11172..11447)
                     /locus_tag="WJL20_000151"
     CDS             complement(11172..11447)
                     /locus_tag="WJL20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ7682362.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11632..12702
                     /locus_tag="WJL20_000152"
     CDS             11632..12702
                     /locus_tag="WJL20_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ7682363.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12809..13756)
                     /locus_tag="WJL20_000153"
     CDS             complement(12809..13756)
                     /locus_tag="WJL20_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ7682364.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13771..14178)
                     /locus_tag="WJL20_000154"
     CDS             complement(13771..14178)
                     /locus_tag="WJL20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ7682365.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14259..14918
                     /gene="ung"
                     /locus_tag="WJL20_000155"
     CDS             14259..14918
                     /gene="ung"
                     /locus_tag="WJL20_000155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ7682366.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHTLLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(15426..15941)
                     /locus_tag="WJL20_000156"
     CDS             complement(15426..15941)
                     /locus_tag="WJL20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682367.1"
                     /translation="MKSKLPFILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(16407..18326)
                     /locus_tag="WJL20_000157"
     CDS             complement(16407..18326)
                     /locus_tag="WJL20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7682368.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGRRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(18403..18798)
                     /locus_tag="WJL20_000158"
     CDS             complement(18403..18798)
                     /locus_tag="WJL20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ7682369.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            18971..20278
                     /locus_tag="WJL20_000159"
     CDS             18971..20278
                     /locus_tag="WJL20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ7682370.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            20271..20705
                     /locus_tag="WJL20_000160"
     CDS             20271..20705
                     /locus_tag="WJL20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682371.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(20764..21033)
                     /locus_tag="WJL20_000161"
     CDS             complement(20764..21033)
                     /locus_tag="WJL20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7682372.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(21215..23665)
                     /gene="lon"
                     /locus_tag="WJL20_000162"
     CDS             complement(21215..23665)
                     /gene="lon"
                     /locus_tag="WJL20_000162"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ7682373.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLEQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            23916..25019
                     /locus_tag="WJL20_000163"
     CDS             23916..25019
                     /locus_tag="WJL20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7682374.1"
                     /translation="MSSFIFPDDGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(25091..26836)
                     /gene="recD"
                     /locus_tag="WJL20_000164"
     CDS             complement(25091..26836)
                     /gene="recD"
                     /locus_tag="WJL20_000164"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ7682375.1"
                     /translation="MERQTDEFAQAAARAAIRFLERYAGSGSEVLANCTERLFQALQN
                     GHSFIRLKKGEASFLSTLKPVVGESASPLILEGEKLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSHKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAISGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFALTVHKSQGSEY
                     REVWLLPPSAGGSDDADDALSGLSRGLLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(26903..27598)
                     /gene="lolD"
                     /locus_tag="WJL20_000165"
     CDS             complement(26903..27598)
                     /gene="lolD"
                     /locus_tag="WJL20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ7682376.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(27591..28838)
                     /locus_tag="WJL20_000166"
     CDS             complement(27591..28838)
                     /locus_tag="WJL20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ7682377.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            29043..29321
                     /locus_tag="WJL20_000167"
     CDS             29043..29321
                     /locus_tag="WJL20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682378.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(29377..29994)
                     /gene="recR"
                     /locus_tag="WJL20_000168"
     CDS             complement(29377..29994)
                     /gene="recR"
                     /locus_tag="WJL20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ7682379.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(30061..31599)
                     /locus_tag="WJL20_000169"
     CDS             complement(30061..31599)
                     /locus_tag="WJL20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ7682380.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQAGGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(31659..32033)
                     /locus_tag="WJL20_000170"
     CDS             complement(31659..32033)
                     /locus_tag="WJL20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ7682381.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGMEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFKRQAPCRQG"
     gene            32183..33811
                     /locus_tag="WJL20_000171"
     CDS             32183..33811
                     /locus_tag="WJL20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ7682382.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     PQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            33883..35136
                     /locus_tag="WJL20_000172"
     CDS             33883..35136
                     /locus_tag="WJL20_000172"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ7682383.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PMREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         7711 a         9077 c         9644 g         8721 t
ORIGIN      
        1 taccgcaaaa cataccataa aagaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggaa taatttattt ggtgcccggg agaagactcg aacttccaca
      301 cccgtgagga taccagcacc tgaagctggc gcgtctacca attccgccac ccgggcaatc
      361 taaatattga aataaagcaa agcatttgat ttggtgcccg ggagaagact cgaacttcca
      421 cacccgtgag gatactagca cctgaagcta gcgcgtctac caattccgcc acccgggctt
      481 tgctttattt gctgttttgc ggtacagtgt cctgccgcaa agaagtcgct attatatagt
      541 tcataaagag aatgtcaaca gtccaaatga ataaaaatat taaatcttta aatttacggg
      601 aaaaagaccc gtttttaagt cgtgaaaaac agcgttatga acatcctttg cccagtcggg
      661 aatggataat cgaattgttg gagcgcaaag gtgtgccttc aaaaatcgaa tcgcttgcgc
      721 gcgagctgtc gattacggaa gacgagtatg tcttttttga acgccgtctg aaggcgatgg
      781 cgcgggacgg tcaggtttta atcaaccgtc ggggcgcggt ttgcgcggcg gacaaatcgg
      841 atttggtcaa atgccgcatc gaggcgcata aggacggttt cggttttgcc gtgccgctca
      901 cgcccgccaa agacggtgat tttgttttgt atgaacgcca gatgcgcggc attatgcacg
      961 gcgatattgt cactgttcgt cctgccggca tggaccgtag gggccgccgc gaagggacgg
     1021 ttctggatat tgtcgaacgc gcgcaaagca aagttgtcgg ccgtttctat atggataggg
     1081 gcgtggcgat tttggagccg gaagacaagc gtctgaacca aagcatcgta ttggaaccgg
     1141 acggcgtggc gcgtttcaaa cctgaatccg gtcaggtcat cgtcggcgaa attgaggttt
     1201 atcctgagca aaaccggccg gcagtggcaa aaatcatcga agttttgggc gattatgccg
     1261 acagcggcat ggagattgaa attgccgtgc gaaagcatca tttgccgcac caattcggtg
     1321 aagcgtgtgc caaagccgcg aaaaaaattc ccaaccatgt acgcaaaagc gatttgaaag
     1381 gccgcgtcga tttgcgcgac ctgcctttgg taacgataga cggcgaaacg gctcgagatt
     1441 ttgacgatgc ggtgtttgcc gagaaaatcg gacgcaatta ccgtctggtc gtggcgattg
     1501 ccgatgtcag ccattatgtc cgccccgatg acgctatcga cacggacgct caggaacgca
     1561 gcaccagtgt ttacttcccg cgccgcgtga ttcccatgtt gccggaaaac ctgtccaacg
     1621 gcatctgctc gctcaatcct catgtcgagc gtttgtgtgt ggtgtgcgat atggttatca
     1681 cttacgcggg caatatcaaa gaataccgct tctaccccgc cgtgatgcgc tctcatgccc
     1741 gcctgaccta caaccaagtt tggaaatggc tttcaggcgg catcgagcat ccgttcaaaa
     1801 cccaaatcga cacgctttac aaactcttca aaatccttca gaaaaagcgt ttcgaacgcg
     1861 gggcggtgga gtttgacagc atcgaaaccc aaatgctttt cgacgacaac ggtaaaattg
     1921 aaaaaatcgt ccccgttgtc cgcaacgatg cccacaagct gattgaagaa tgtatgttgg
     1981 cggcaaacgt ttgcgcagcg gattttctgt tgaaaaacaa gcataccgca ttgttccgca
     2041 accatttggg gcccacgccc gaaaaactcg ccgccttgcg cgagcagctc ggtctgttgg
     2101 ggcttcaact tggcggcggc gacaacccgt cgccgaaaga ctatgccgcg cttgccggac
     2161 agttcaaagg caggccggat gccgaattgc tgcaagtcat gatgttgcgc tccatgcaac
     2221 aggcggttta cgaaccgcat tgcgacggac actttggtct tgcctacgaa gcatacgccc
     2281 acttcacctc gcccatccgc cgctatcccg acctgaccgt acaccgcgcc atcaaagccg
     2341 tgttgaatca gcaaacctac acgccaaaaa aaagctggca ggctttgggc gtgcatacct
     2401 cgttctgtga gcgccgtgcc gacgacgcca gccgcgacgt ggaaaactgg ctgaaaacct
     2461 actatatgcg tgacaaagtc ggcgaaatat ttgaaggcaa aatttccggg gtggcaaatt
     2521 ttggaatatt tgtcactttg gacgatatcc atatcgacgg tctggtgcat atcagcgatt
     2581 tgggcgaaga ctatttcaac ttccgccccg aaatcatggc aatcgaaggt gaacgcagtg
     2641 gcatccgttt caatatgggc gacagggttg ccattaaagt ggcccgtgcc gatttggatg
     2701 atggaaaaat cgattttgtc ctgattgccg aggggagcgg cagggggcgg aaagttaaat
     2761 catccgcgtc tgccaaaccg gcagggacgg cggggaaagg gaagccgaaa accgccgccg
     2821 ggaaaaaaac agcccgaggc ggcaaagtaa ggggaagggg cgcgtctgcc gccgcagaat
     2881 cggggaaaaa ggcaaagaaa ccggttccga ttaaggtaaa aaaacggaaa ggcaaatcat
     2941 aatgctgacg gggcggcttg gggagggcgg ggcatacttt cccgaagccg ccgcccgatt
     3001 ttttgatatg gcggccggcg catcatttca ggccaatcga aacgcccgta ttgaaagatt
     3061 gcgtttattt ccaccgccgt tttaaaggcc ggcggtattc ggcagacggg gcgcaaacgg
     3121 cgttcagacg gcattttcat tctttcggcg tgtccgtccg aattgctttg cccgtccgcg
     3181 caatcagccc gtgcggcttg ccttgaacgg acaaaaaatg ccgtctgaaa cccgaaaatc
     3241 aggtttcaga cggcattttg ccttgaaaag gctgttcaaa tcagcggtgg tagttcggtg
     3301 cttctttagt gatttgaacg tcgtgaacgt gcgattcgct catacctgcg gaagtgattt
     3361 ccacaaattc ggctttttcg tgcatttcgg caatattggc gcaacccaag taacccatac
     3421 tggaacgcag accgccggtc agctggtgga tgatgttcac aatcgggcct ttgtaaggaa
     3481 cgcggccttc gatgccttcg gggacgtatt tgtctgcgct gtcggttttg tcttggaagt
     3541 agcggtcggc agaaccttgg ctcatcgcgc ccaaggaacc cataccgcga taggatttgt
     3601 acgagcggcc ttggtagagt tcgatttcgc ccggcgcttc ttccgtgcct gcaaacatac
     3661 cgccgagcat gacgctgtac gcgcctgcgg cgagggcttt ggcgatgtcg ccggagaagc
     3721 ggatgccgcc gtcggcaatc agcggaacgc ccgtgccttt gagggcttcg gcaacgttgt
     3781 gaatggcggt cagttgcggc acgccgacac ctgccacgat acgggtggtg caaatcgatc
     3841 ccggaccgat accgactttg acggcatccg cgccggcggc gaccaaatcc aatgcggctt
     3901 tggcagtggc gatgttgccg ccgatgactt ggatgtgtgg ataggtttct ttcacccatt
     3961 tcacgcggtc aatcacgcct tggctgtgcc cgtgggcagt atcgacgaca atcacgtcca
     4021 cgccggcttc gaccaaggct ttgacgcgct cttcggtatc gccgccggtg ccgactgccg
     4081 caccgacgcg cagacggccc tcggagtctt tgttggcgtt gggaaactcg gtggttttta
     4141 aaatatcttt aacggtaatc agacctttga gttcgtcttt ttcgttcaga accaaaacgc
     4201 gctcgacttt gtgcgtgtgc atcagttcgc gcgcttcgtc tatgcttgtg ccttcgggga
     4261 cggtaaccag acgttcgcgc ggggtcataa tggcggaaac gggcaaatcg acgcggtttt
     4321 caaaacgcag gtcgcggttg gttacgatgc cgacgacttt gccgttttca acgacgggca
     4381 ggccggacat tttgcgtttg cgctgcgcgc gcatttccaa gacttcgcgg atgagcgtcg
     4441 tcggtgcaac ggttacgggg tctttgacca cgccgctttc gtggcgtttc actttggaaa
     4501 cggcgcgcgc ctgcatttcg ggcggcatgt ttttatggat gatgccgatg ccgccttcct
     4561 gtgccatcga aatggcgagg cgcgcctcgg taacagtgtc catcgcggcg gaaagcaggg
     4621 ggaggttaag tgtgatttcg cgggtgagct tggtttgaag tttaacgtct cgcggcagca
     4681 cggtcgaatg tgcgggaacc aacaaaacat cgtcgaaagt ataggctttt tctacgatac
     4741 gcataatgct cggtctttca gtttgtgcaa gatgcacggc attatagcac gttaccggcg
     4801 gcttgacagt ttatattgtt tgcaatatcc cccgccttca gacagcgtac ggcgcggcgg
     4861 cgggataccg tctgaaaacc ggcagggtgc ttatgtcaac tgttccagca aagcctgctt
     4921 caatgaggac tggatttttg ggtttttcag gtcggggcta aaaacggtaa aactgtcttc
     4981 ggcgcgttca tccagtgtgg agattttggc atagcgcagg ctgacgttgt gggcgaaaaa
     5041 gacttccgcc atatcggcga gcaggaaggg gcggttgacg gcggtaattt cgacggagta
     5101 ccagtccgga tagtcttctt cgggggtgat ggtgatgctt ggcgcgatcg gcatgtagcg
     5161 gctgcggcgg ctgatgcggc ggctatggct ggagatttcg gcaacggtgt gtccgtggat
     5221 aaagctgttg agttcggctt cgagcgcgct ttggatgtcg gggtagtctt cgggagcgtg
     5281 ctgcgagggg atttgcacga tgaaggtgtc gaggatgtag tcgtgttcgg taataaaggc
     5341 gcgggcggcg aggatatcga agccgtggcg gctgaagatg cggcagaggc gggcgaacag
     5401 gcgcgggccg ttgggcatga aaaccatgac ttgaaagctg tcgcttttgg gcaggatgcg
     5461 gctgcggacg atgggggttt caaagtcgtg gactaggttg gcggcgtgcc acaggatttc
     5521 gcgggactgg tggcgggcga agtaggcgga accgagcgcg ttccatagtt ttttctgctg
     5581 tttttcgggg acggcggcgc gggtgagtaa gtcggcggct tcctgccggc ggcggccgaa
     5641 gagggtgtgc gggttgccgc cgttgcctgt aaggtagcgt ccggcggcat ggaagaggct
     5701 ttccagcagg ctggcgcgcc atgcgttcca cagcttggga ttggtgccgc gtatgtcgga
     5761 aatggtcaga aggtagagcg cgctgaggcg ttcgtgggtt tggacgcgtt tgcagaaagc
     5821 atcgagtacg tcggggtctt ggatgtcttc tttttgggcg acggcagaca tcagcaggtg
     5881 gttttcgacc agccaggcga gcaggtcgct ttcttctccg gtcaggaagt ggtcagcggc
     5941 aaattggcgc gcgtctgcga tgccttgtat ggcgtggtcg ccgccgcgtc ctttggcgat
     6001 gtcgtggaag aaggcggcga gatagaggat gtcttgtttt tcaaaggact gcatcagcgc
     6061 ggaggcatag ggcagctcgt ggctgtgcat atccaaggca aggcggcgga cgttgcggac
     6121 gacggcgagg atgtggtcgt ccacgggata aatgtggaac aggtcgtgct ggagcaggcc
     6181 gacgattttt tcccacgcgg gcaggtagcg tcccaatacg ccgtagaggt tgaggaagcg
     6241 cagggtttgg gtcagtccgt tgccgttgcg gaaaaagccg gcgaagcggt ggcggttttc
     6301 agaattttgg tagaagctgc ggttgatttt gcgcgtcgcg ccccaccatg cgcgcagggt
     6361 ttgcggttcg agcgcggtaa tatcgttgcg ctgctgcatg atttcgacga ttttgaaaat
     6421 gtgttcgggc cgtctgaaaa aaatatcggt gtgccgcgcg gcgatttggt tgttgacttg
     6481 gatgtagtcg tcgtcaatcc gcagggtaac gcgcaacggg gtggaggaaa cgcggctttg
     6541 cagcataggc gtgaggatgc cgcccagttg tttgacggtt ttaatcgcgc ggtaaaacac
     6601 gcgcatcagt tcttcgcttt ggcggcggag gttcaagcct tcataaccca tgctttcggc
     6661 gacttgcggc tgcaaatcga acagcaggcg gtcttcggcg cgattggcgt ttaaatgcag
     6721 atggatgcgg atgtgggcga ggcggcggta gccgtgcgaa agcataccgg cttcggcacg
     6781 cgtcaaaatc cgctgtttga gcaggtcggg caggtcggtc gccaagcctt gcgccttcgc
     6841 tatccaaagc agggtgtgga tgtcgcgcag accgcccgga cagcttttga tattcggctc
     6901 taatactgcc cccgaacctt gcgatttggc gtggcggtgt tccatctcca ccagttttgc
     6961 ctcgacaaac gccgccacat tgcgctgcgc gttcattttt tccgctagtt tatccgccgt
     7021 ttggcggttg ccaaacaaaa acctagcctc taaaaacgct gtgtcccccg taatatcatt
     7081 gcgcacgctt tcacatagtt catcaacgct gccgcttttt acagacggca tcagtttgca
     7141 gtcccacagg gtttgaacaa atcgggcgat ttgttcctga atgccgtctg aaagcgggga
     7201 aggggagaca accgccaaat ccacatccga acagggatac agttcgccgc gcccgaagcc
     7261 gcctaccgcc atcaggcata acgcgctgtt ttgaaaatac tctgcccaca atgccgccag
     7321 caagatttcg actgccgctg tgtattccct gaaaaatacc gacacgcgct tggctttcaa
     7381 ataatgcgct tcggcggcat cgcgctgccg tttgaaggtt tccagtgctg aagacaggtt
     7441 tgcaggcatt tttattcttt cgattggcgg gaaaaaagga gacggatggt tcggcggtca
     7501 aataccgctt tcagacggca tttgtcgggt atcggcggaa ttggtcatca ggttcaaccg
     7561 ttcctgcggc ggcagcagca actgatacgc cgccacgccc aaagccgccg ccattttttc
     7621 gatattcgac agggcgatgt tccagcgttt gcgttcgact gccgacacat aagtcctgtc
     7681 caaaccgcat tgccgcgcca attcttcttg cgaccaaccc ttgttcacgc ggaaaagccg
     7741 catattgtat gccaataccg cccgcaaatc ctgttcgtca ggcagttcgg caggcggggt
     7801 caatttgttg cccatcattt gcttccagat aaatggttaa aattaagcat ttgctgttac
     7861 ggattttact tcacataaaa gccaaaaaaa aggggtggcg gcagccacac ccaaacacac
     7921 acacatcaag aggaaagagg taaaatcaag acagattggg aaacagcaaa caagcaatct
     7981 agatttcata tcggtattat caaacaaaaa ccgttatcga gtattgattc aaatcaattt
     8041 agattttatt cgcaaaccgg caaagaaaaa cggcataccc gtttatacgg atatgccgtc
     8101 tgatgatgca ggggactgct gtatttggca gtggtttgtg ttttagtcct cttgcgccgc
     8161 tgtttgcagg tagttcggca aaccaatttt gccgattagt tcctgctggg tttcgagcca
     8221 gtcgatgtgt tcttcgttgg tgtctttttg tttttccaac aaatcacggc tgacgtaatc
     8281 ctgttgcgct tctgctgtgg cgatggcggc aagcagggct tcgtgttttt cctgttcttt
     8341 ggtcaaatcg caggcgatga tttcttcggt ggactcgcca atcagaagct tgcccagttc
     8401 ttgcaggttc ggcaggcctt caaggaacag aatgcgctcg atcaaatcgt cggcagcttt
     8461 catttcaacg atggactgtt tgaagaaatg ttcgcccagt tcttcaaagc cccagttttt
     8521 caaaatacgg gcgtgaagga aatattggtt gatggttacc agcagcaagc ctaagttttt
     8581 gtttagctcg cggataacca gacggtcgcc tttcatacgg actcctttta ttccgaatac
     8641 ggattacagt tggctttggt agtagttgcc ttcgccaatt aactcgatca ggcgaagctg
     8701 ctgttccagc cagtgggcgt ggtcttcttc ggtatctttc agttgggcaa ccatcaggtc
     8761 gcgcgtaaca tagtcttgag cctcttcgca cagtttgatg ccttttttca acgcatcacg
     8821 tacttcatat tcggtttgca ggtcggcttt gaggcaggaa accacgtccg tgccgatatt
     8881 cagttcggcg cgtgccattt tcggcgtgcc gcccagcatc aggatgcggc ggatgaagtc
     8941 ttcggcgtgt gtggtttctt cttccatctc gtggttgaga cgttcaaaaa gtttggtgta
     9001 gccccattcg gagtagaggc gggagtggat aaagtattgg tcgcgtgccg ccagctcgcc
     9061 agacagcaat tcgttcatat aatcaacaac agcttgattg ccttgcataa tatctctctt
     9121 tctgtaactt ggttttcggt atgtctgaac agcatctgtg tcgctattcg ggatgcgtgc
     9181 attgtatgca aaagctgtcg gcatgacaaa ttttctattt aaaatacaaa caattatcaa
     9241 aagaaatagg gcgtattccc cgatgccttc caaatcaatg ctctgttttc tatcggtttt
     9301 tttgctgcta aaactaagaa tccatctcat ccataaagaa catttacact tattaaccat
     9361 agcgaaaaac aaatagagca cggtttttta tcaaaattta taatgaatcg ttctcattaa
     9421 ccgacagatt cttttgagat tatcggattt tgggaataaa ttatgccgtt tgaggggctt
     9481 tcagacggca tagacaatta aagctgatta aacaacgcgg cagacagcag cgtaggaaat
     9541 tattttttac ttcggaacgg tattttgggt ttaatgatgc ccgcaaccgc cgtggctttc
     9601 gcgtaaggct tcggcagcct gttcgtcggc gtggtagctg gaacgtacca tcgcgccgat
     9661 ggcggcattg ctgaagccca gttcgtatgc ttctttttca aagattttga actgctcggg
     9721 cgtaacgtag cgcaggacgg gcaggtgtcc gtctgaaggc tggaggtact gtccgatggt
     9781 aatcatttcg atattgtgcg cccgcatatc gcgcataatt tcacgcacgt cttcgtctgt
     9841 ttcgcccaag ccgaccatga tgccggattt ggtcgggatg tgcggcatca tttctttata
     9901 acgttttaat aagtctaaag aatgttgata attggcaccg ggacgggctt ttctgtacag
     9961 gctcggatgg gtttctaggt tgtggttcat cacgtcgggc ggggtttcgg caaggatttt
    10021 gagtgcgatg tccaagcgtc ctcggaagtc ggggacgagg atttcgattt tggtgttcgg
    10081 gctggtttcg cggatggctt tgatgcagtc ggcgaaatgc tgtgcgccgc cgtcgcgcag
    10141 gtcgtcgcgg tcgacggagg tgatgacgac gtaacgcagg ttcatggctt tgacggattc
    10201 ggcgaggttt ttcggttcgt cggggtcgag catattgggc cgaccgtgtc ccacgtcgca
    10261 gaacgggcag cggcgggtgc agatgtcacc cataatcatg aaggtcgccg tgcctttgct
    10321 gaagcattcg ccgatgttgg ggcaggaggc ttcctcgcaa acggtgtgca tcttttgttc
    10381 gcgcaaaatg tctttgattt caaagaattt gcgcgatggg agtttggcgc gtatccattc
    10441 gggctttttc agtttctcct gaagggggac gactttgatg gggatgcggg cggttttgtc
    10501 cgcgcctctg agtttgatgc cgcgtttggg gtcgtcggtt ttgatttcac tcattgttgt
    10561 ctgctttcgg tgtgaattgt gtttcaaggt gtgcggtaag tttggcggcg acttcgtccg
    10621 gcgtggggca gggttggaca aaatccgcga tttgcgtcat ttccataccg gcgtagccgc
    10681 aggggttgat gtgggtaaac gggcttaaat ccatattgac gttgagcgca agcccgtgat
    10741 agacggagcc gtttttgata cgcagcccca gtgaggcgat tttgcgttct ccgacataaa
    10801 cgccggggcg tttggggtct gccgccgctt cgatgccgta ttctgccaat gtggcgatga
    10861 tgctgttttc aagcgcggaa acgatgttgc ggacgctggt tttgcgccgt ttgaaatcaa
    10921 tcatcgtata aacgaccaat tgcccgggcc cgtgataggt aatctgcccg ccccggtcga
    10981 tttggacgac gggaatgtcg tcgcgaatca gcaggtgttc gggttttccc gccagtcctt
    11041 gtgtgaacac gggcgggtgt tcgacgaccc acagttcgtc ttcggtgtca gcattccgtc
    11101 cggcattaaa ggttttcatc gcttcaaaag tcggcagata ttcgaccaaa cctttgtgta
    11161 tgattttcat ctcaaagtac cactttgacc agttcgtgcg aagtcagcgc gcggtagatg
    11221 ttgtccaatt gttcttggtt ttcaaccttt acctgaacgg tggcgccggt atagttgcct
    11281 ttgctgctcg gacgcgtggt gatgtggtgc gcctgcgtgt cgggggcgtg gaggcggacg
    11341 gtgtctaaaa ccgcctgctc gaactcggga tgcaccgcgc ccattacttt caatgggaag
    11401 gtgcagggaa attcgatgag ggatgttttg tttttttgtt cggtcatgat gtgctgcctt
    11461 gtcgtgtacg gtatgccgtc tgaaggcggg tttgcctttc agacggcatc ggatgtgcgt
    11521 tattttagcc taaaccgcga taacaggcta tcgggaaggc ggaggctttt ttgacggcgc
    11581 ggcggttctg ctatactggc gcacaatatt atttttagaa gggtggtttt tatgtatcgg
    11641 aggaaagggc ggggcatcaa gccgtggatg ggtgccggtg cggcgtttgc cgccttggtc
    11701 tggctggttt tcgcgctcgg cgatactttg actccgtttg cggttgcggc ggtgctggcg
    11761 tatgtattgg accctttggt cgaatggttg cagaaaaagg gtttgaaccg tgcatccgct
    11821 tcgatgtctg tgatggtgtt ttccttgatt ttgttgttgg cattattgtt gattatcgtc
    11881 cctatgctgg tcgggcagtt caacaatttg gcatcgcgcc tgccccaatt aatcggtttt
    11941 atgcagaaca cgctgctgcc gtggttgaaa aatacaatcg gcggatatgt ggaaatcgat
    12001 caggcatcta ttattgcgtg gcttcaggcg catacgggag agttgagcaa cgcgcttaag
    12061 gcgtggtttc ccgttttgat gaggcagggc ggcaatattg tcagcagtat cggcaacctg
    12121 ctgctgcttc ccttgctgct ttactatttc ctgctggatt ggcagcggtg gtcgtgcggc
    12181 attgccaaac tggttccgag gcgttttgcc ggtgcttata cgcgcattac aggcaatttg
    12241 aacgaggtat tgggcgaatt tttgcgcggg cagcttctgg tgatgctgat tatgggtttg
    12301 gtttacggct tggggttggt gctggtcggg ctggattcgg ggtttgccat cggtatgctt
    12361 gccggcattt tggtttttgt tccctatttg ggcgcgttta caggactgct gctggcaacc
    12421 gtcgccgcct tgctccagtt cggttcgtgg aacggcatcc tatcggtttg ggcggttttt
    12481 gccgtaggac agtttctcga aagttttttc attacgccga aaatcgtggg agaccgtatc
    12541 gggctgtcgc cgttttgggt tatcttttcg ctgatggcgt tcgggcagct gatgggcttt
    12601 gtcggaatgt tggccggatt gcctttggcc gccgtaacct tggtcttgct tcgcgagggc
    12661 gtgcagaaat attttgccgg cagtttttac cggggcaggt aggcggttcc gaaacatatt
    12721 tgaagcggaa tacaaccttg cccgggttta aataaaaatg ccgtctgaac cccgaaaaca
    12781 gagcttcaga cggcattttc atcacggctt atttggcggt tttgctgctg tcgataattt
    12841 tcataccggc agaaatcagg ctgccgatgt cggcaacatt ggcgggcata atcagcgtat
    12901 tgctttcttt ggcaagattg ttgaacgcgg cgacgtattg ttccgcaatc ttcagattga
    12961 ccgcatccgc gccgccttgg gtttgaaggg cggcggcaat ttgacggatg gcttcggcat
    13021 tggcttcggc aacaaggcgc aaggattccg cttcaccttt ggcgcggttg atgcgggcga
    13081 ttttctcggc atttgacgca ttgaccgcag cctgagcctc gccttcggat tgttggattt
    13141 cggcttcgcg ctgaccactg gcaaggttga tttgttcgat tttacgacct tcggattcgg
    13201 cgatacgggc gcgtttttcg cgttcggcag taatttgcgc ctgcattgag cgaaggattt
    13261 cttgcggcgg aaccaagtct ttaatctcat aacgcaaaac cttcacaccc caagccccgg
    13321 ccgcctcgtc caaagccgca acaacagtac tgttgatttc gtcgcgttct tcaaacgttt
    13381 tgtccaactc catacgcccg ataacggaac gcagcgtcgt ttgggcaagc tgggtaattg
    13441 ccatgatgta gttgctcgaa ccgtatgagg cgagtttggg gtcggttact tggaaataga
    13501 tgatgccgtc aacagtcagc tgcgtattgt cgcgcgtgat gcagacctgg ctgggtacgt
    13561 ctaaagggat ttctttcagc gaatggcggt aggcaacgcg gtcgataaag ggaatcaaaa
    13621 tattcaaacc ggccgtcagg gcgcgatgga aacgcccgag cctttcgaca acgtggactt
    13681 cctgttgtgg gatgacaaca aaggatttga aaccgaaaac ggcaacggct gccaacaaga
    13741 taatgaaaaa ttccataatt cctccgagtg ttaaggtttt gcgatgataa gaaggttgcc
    13801 ttccttgcgg acgattaggg cgcgcgttcc tggttcaagc tcttcttgcc ccgtattttg
    13861 cgcctgccag tgcgtaccgc gataaaaaac ttcgtaacgg ttgccgcctg tgtgtcggag
    13921 gatttcgaca tattgtccgg catccaaatc ctgatatgaa tccgtttcaa cttttctaac
    13981 ggcggttttg gcgtgtacga accaaatacc cagcgcggaa agcagagcgg cggtcaagac
    14041 ggcggcaggc gtgctgccgg tcagcccgta agcaatgccc gaacccgcca aagccgcgct
    14101 gacaaccaaa agataaaccg ttcccgtcaa taattcgatg attaagacgg caacagcagc
    14161 aacaaaccat acagtcatac atttccccac aaagcgcgtc gtttgacaaa atcacgcgat
    14221 atcagcagta tagccgaatt tgaaaggata gggcagatat ggacacttgg cacgatgcac
    14281 tcggcggcga aaaacagcag ccgtattttc aggaaatttt aaatgcagtc aggcaggaac
    14341 gtttgtcggg acaaatcatc tatccgccgg cggcggatgt gttcaacgca ttccgcctga
    14401 cagcgttcga ccgggtcaaa gccgtcattc tcggacaaga tccgtatcac ggggcagggc
    14461 aggcgcacgg tttggcattt tccgtccggc agggtatccg cataccgccg tctttactca
    14521 atatctacaa ggagttggaa accgacatcg aaggcttttc cattcccgcg cacggctgtc
    14581 tgacagcgtg ggcggagcag ggcgtattgc ttctgaacac ggttttgacg gtgcgtgcag
    14641 gacaggcgca ttcgcacacc cttttaggct gggaacgctt taccgatacc gtcatcaggc
    14701 agcttgcgac acaccgcaag caccttgtct tcatgttgtg gggtgggtat gcacaacaaa
    14761 aagggaggct gatagacagt caaaatcatt tgatattgac cgcaccgcat ccgtctcctc
    14821 tgtcggcata tcgcggtttt ttcggctgcc gccatttttc acaggcaaac agctatttga
    14881 gccggcacgg tatcgatccg ataaactgga agctgtgaat gccatctgaa tgccaagttg
    14941 cttaaacccc actgaaggct gcttttgaaa ccggaaacaa agtgattaag gaaaaccggc
    15001 ccataatgca aaaccgtctg aatcctaggg ttcagacggc attttttgcc gccatttcca
    15061 aaaacaactt ttgttcgcct gccgacaaat ccgacaaagt cgtttggttg agggctttgg
    15121 caataattta acataatata cattatgcga actatcggaa acagttgtac gtgtccctgt
    15181 ggtctttcca agtaggaaaa ttaaagtatg gacaatgcgg ctgaatgtat agcccggagc
    15241 atccgcgtat ccgaaggtac tgaacgatat gccctgcatc cctttcgatg cttccatcca
    15301 gccggtattg ggaaatacgt tgaccttcaa tataatatac aggcagccga cacacaggat
    15361 atgccgctgc tttttcatca tttcttctgt caaatccttg gaacggtcga tttgaaaaca
    15421 cggctttaca tacgtcccaa ttctctttgc agtgcttgga tattttgctg cctgtccaat
    15481 acattgcttt gtaatgcatt tatttcttga tggttgatgt tgccgccctt tgccagacgt
    15541 gcttgtgata acattttttg ggcttcagtc agggctttgc gttcgttgct caattctgct
    15601 tcgagaatgg agcgtctgct gttgtttaca ggtgcttgtt gaggcggggg cgtattggat
    15661 tttgccggct tggatactgt tttgaccggg gctttatatt tgacagcctg tccgccgttt
    15721 gacggtgatg gtgtcggttc ggatgtttgg ggcgggatat agcgttcgct gctgtagttg
    15781 ccgattgggg gcaaatcggt tgagaggcag cttttggacg gcttggtggt gtaaacggtt
    15841 tctccgttga ttgtgcaggt gtagattttg gccgcattcg cacccaatgg gcttgaaatc
    15901 agggaaaagt tgattaggat gaaggggagt tttgatttca taatgtgctt gatgctcgaa
    15961 taattatttg attttttggt attttttgta atgctttctt ggcgttttac gggcttttct
    16021 tttcggttgg ttgcttttga ttttctttcg gggctttggc agcctgcctg ttctttttcc
    16081 cggcaggttt tttgtttttc tttttacttt ctatgtgtat tttggcttct taactgagtt
    16141 ttttaatttt caggcggtat ccgcctccct gatggctgct gattttaggt aaatccgcat
    16201 cggcgcacga tcctgctggg gctgatgtat atacgctttt gccatttgag ttgcaatggt
    16261 atacggaggc ttgcgccgcc gcgccagaaa gggacaggag acccaaggcg gcaaaaagtc
    16321 tgatgtttat accggtaaaa ggcggtgata acgcccgaat tataccgtta ttggcaggca
    16381 aagataagca ccctgcccgc gcttctttat cgctcggcaa actgtttctc gatttcagtt
    16441 tcaatccgtt ccaattcttc ttgacaagcc agccaaacct cttcgatttg ggcaagttgt
    16501 gttttgactt ttgccagctg ggataaggta ttttgcaatt tttctttgtt tgcttcaagg
    16561 taagcgtctt cttgtgctaa aaatgcttca catgccgtct gaatttcgga aagctgcgcc
    16621 atttcttttt cggcacggtc tattttctgc tgtatcggct tgccgcgtcg ggctttttcc
    16681 tgacggattt gcgcttcgat gcgcttggtg tctttgcggc tttggctttg tgcggatgct
    16741 gctggtgcgg cggcgttttc ctgtgccaaa cgccattggc ggtaatcgtt caaatcgccg
    16801 tcgaagttct tcagacggcc tttgtcgatc aggaggaagc tgtcggtagt cgcttcaagc
    16861 aggctgcggt cgtgcgatac gatgattaag gcgccttgga aactttgcag agcgagcgtc
    16921 agggcgtggc gcatatccaa gtccagatgg ttggttggtt cgtcaagcag cagcaggttc
    16981 ggcttttgcc agataatcat ggcgagggcg aggcgggctt tttctccgcc ggaaaaaggt
    17041 tcggttttct gtaacgccat atcgccaaca aaattgaagc cgccgaggaa atttcggatt
    17101 tcttgttcgc gtacttcggg agaaagctgc tgaatatgcc aaacagggtt ttggtcggcg
    17161 cggatggtgt cgagttggtg ttgggcaaaa tagccgatat tgagtttttc ggaacggatg
    17221 atgctgccgg aaagcaaatc gattttgcct gccaaggctt tgataaaggt ggatttgccg
    17281 ctgccgttga cacccaatag cccgtagcgt gcgccgcttt ccagcgacag ggtaatgtcg
    17341 tgcaggacag ttttgccttc gtaacccaaa tcagcgtgtt ctagctttaa caaaggattg
    17401 ggcagatggt cgggatggta aaactcaaag gaaaactcgc tgtccagatg cgcgggagcg
    17461 atgcgttcga gcttcgccaa agccttcatg cggctttgcg cttgaacggc tttggtggct
    17521 ttggctttga agcggtcgat aaaggattgc aaatgtttga tttgcgcctg ctgtttgaca
    17581 taggcagctt gttgttgcgc gagacgctgc gcacgttcgt tttggtaaaa atcgtaattg
    17641 ccgccgtatt gcgtgagttt ttgctgcgat aattcaatgg tttgggtagt tgccgcgttg
    17701 agaaaatcgc ggtcatggga aatgatgatt tgcgtgcagg gtaaagaagc aaggtggttt
    17761 tccagccaca agacggtttc caaatccaag tggttggtcg gttcgtcaag caagagcaaa
    17821 tcggcgcggc aaatcagggc ttgcgcaaga ttcaggcgca tacgccagcc gccggaaaag
    17881 gatttgacgg ggcggctgtg ttcttcttgc gaaaaaccca gcccgttcaa caattttgcc
    17941 gcacgcgccg gcgcggtata agcgtcgatt tcttccaatt tagcatgata ttccgcctgc
    18001 ttcatgccgt cattttgcgc ttctgcctgc ctcaatgccg tctgaaaagc ctgcaactcg
    18061 gcatcgccct gcaaaacgta atccaaagcg gaaatatcca aatcgggcgt ttcttgggaa
    18121 acggaagcga gccgccagtt tttcggaatc gagacatcgc cgccgtcctg agtgatttca
    18181 cccttgatta aggcaaacag gctcgatttg cccgttccgt ttttgccgat caaaccgacg
    18241 cgccgaccgg gattgacggt agcgttggct ttgtcgagca ggactttcaa accgcgttgc
    18301 agggtgaggt ttttgatttc aatcataacg gaaacatcgt cgggcgggaa aagcccgtat
    18361 tttacctgaa agtcagtgcc gatgccgtct gaaacgggaa atttacggct gaagccaagc
    18421 ccaagccctg cgcccttccg agtgcaggaa aaccaatgtc ctgaatgccg aatcggtatt
    18481 catgcattcc acgctgattc cgattcggga aaaatccgcc atgattttgg gatggataaa
    18541 ctcctgagcc gcgcccgtcc cgataatcaa tatttccgga tagtcaacag gtttgacgtc
    18601 ggacaacagg ttttccggag tcagatcgga caaggttcgg cattgcgaca ggcagaccga
    18661 atccttatgt acaagcacgg gtttatggaa actttgcccc gccagccgga ttccgcccgc
    18721 accgcattca tattccgcaa actgtccgtc tatcggattt tcttcaaaca acattttttt
    18781 accccgttgc cgcatcatct acaccgaaag ggatgcaaaa tcagacaaat tcatgtagga
    18841 ttggcagatt tcatctgacc cgcctgccga tttcagacgg catttgattc aaagtgcggc
    18901 acaattatat cggcagcgga tattttcgtc tttcaatatt tacatttcag tcggcttaca
    18961 aggagacaca atgaagccag taaacatcgg tcttttaggt ttgggtacgg tcggcggcgg
    19021 tacggctgcc gtgttgcggg acaacgcgga ggaaatttcc cgtcgcttgg ggcgcgaaat
    19081 ccgtatttct gccgtgtgcg atttgagtga agaaaaagcc cgacaaacct gcccgtccgc
    19141 agcctttgtc aaagacccgt tcgaactggt cgcgcgtgaa gacgtcgatg tcgtcgtcga
    19201 attgttcggc ggtaccggca ttgccaaaga tgcggtgttg aaagccattg aaaacggcaa
    19261 acacatcgtt accgccaaca aaaaactgct cgccgaatac ggcaacgaaa tcttctcgct
    19321 ggcggaagaa aaaaatgtca tggtgcaatt tgaagccgca gtagcgggcg gtatcccaat
    19381 catcaaagcc ctgcgcgaag gtttggcagc aaacaggatt aaatccatcg ccggcattat
    19441 taacggcacc agcaacttca tcctctccga aatgcgcgaa aaaggcagcg cgtttgccga
    19501 tgtactgaaa gaagcgcagg cattgggtta tgccgaagcc gatccgacct tcgacatcga
    19561 aggcaacgat gcgggccata aaatcaccat catgagcgca ctggcattcg gcacgccgat
    19621 gaacttttcc gcctgctacc tcgaaggcat cagcaaactc gacagccgcg acatcaaata
    19681 cgccgaagaa cttggctatc gcatcaaact gttgggcatt acccgcaaaa ccggcaaagg
    19741 catcgagctg cgcgtccacc ctaccctgat tcccgaaagc cgcctcttgg caaacgtcaa
    19801 cggcgtgatg aacgccgtgc gcgtcaacgc cgatatggtt ggcgaaacct tatattacgg
    19861 cgcgggcgcg ggcgcattgc cgaccgcttc cgccgtggtt gccgatatca tcgacatcgc
    19921 ccgcctggtt gaagccgata ccgcccaccg cgtaccgcat ctggcgttcc aacccgcgca
    19981 agtccaagcg caaaccatcc tacctatgga cgaaattacc agcagctact acctgcgcgt
    20041 ccaagccaaa gacgaaccgg gcacgctggg gcaaatcgcc gcgctgttgg cacaagaaaa
    20101 cgtgtccatc gaagcactga ttcaaaaagg cgtgattgat cagaccactg ccgaaatcgt
    20161 gattctgacc cacagcacgg tcgaaaaaca catcaagtcg gcaatcgcag ccatcgaagc
    20221 actggattgc gtggaaaaac cgattaccat gatccgcatg gaaagcctgc atgactgagc
    20281 cgaaacacga aatgccgacg gaagagcagg ttgccgcgcg caaaaaagca aaagccaaaa
    20341 tccgcaccat ccgcatttgg gcgtgggtca ttttggcgtt gctcgcttta accgccctgc
    20401 tctcccaatg cgcgatgtcc aaaccgcagg caaaacagaa aattgtcgag tcttgcgtga
    20461 agaatattcc gtttgccgaa aaatggcaaa acgatttgcg ggcccgcggt ttagattcaa
    20521 acaatacccg cctcgccgtc gactactgca aatgtatgtg ggagcagcct ttggacagat
    20581 tgagcgagaa acagattaga tccttcggca aactcggcgc acaagaacag cttgacctgc
    20641 tcggcggcgc aaatgccttt gaagcacgtg acaagcagtg tgttgccgat ttgaaatcag
    20701 aataatgtgg accgataaaa aagccgattc tttaaagaat cggctttttt cataaaaaac
    20761 ggcttacagt gcgtctttca atgctttacc tgcacggaat ttaggcgttt tggcggcggc
    20821 aatggtcaga ggctcgccgg ttttggggtt gcggccttgg cgttccgcac gttcgcccac
    20881 gtagaaagta ccgaaaccga ccaaagtaac ggtgtcgcct tgtttcaggg cggtggttac
    20941 tgcattggta gtggcatcca aagctttttg tgcggcggct ttggaaatgt cggcttcttg
    21001 agcaatcgct tcgatcaatt cagacttgtt cacaatcagt cccttcctgt cttaaaaaat
    21061 gatgaaatgc ccgaatactc ggggtttgta ctgcttgagc aactttcgct ttatagcaat
    21121 tctgaaattg ccgtgtcaag caaaaaatac ggaatcaccc tatttgacag gctttcagga
    21181 cgaaaccgca tttttacaac acatttcctg cgtttcaatg tttggttgcc ctgctgcggg
    21241 gttttggttt tgaagcggat tccgccgccg cttccgcacc agaaggttct gcccaaggct
    21301 caggctggct ttccaaaccc agagccaata cctcgtctat ccatttgacc ggatggatgg
    21361 tcaggccggt tttcacgttt tcagggattt cttccaagtc tttgaggttg tcttttggaa
    21421 ttaaaacgtg tttgatgccg ccgcgaagcg cagccaacag tttttccttc agaccgccaa
    21481 tcggcaggac ttcgccgcgc agggtaattt cgcccgtcat ggcaacatcg gcgcgcaccg
    21541 ggattttggt gaatgcagac accatcgcca aagtcatggc aatgcccgca ctcggaccgt
    21601 ctttcggcgt cgcaccttcg ggaacatgga catggatgtc ttttttctcg taaaaatcag
    21661 gagccaagcc cactgattcc gcgcgggagc ggacaaccga ccacgctgcg gacacggatt
    21721 ccttcatcac atcgcccaac tggccggtgc actgaatcac gcccttaccc ggcaatgctg
    21781 cggcttcgac ggtcagcaat tcgccgccga cttccgtcca cgccaaaccg gttacttgtc
    21841 cgatacggtt ttcgctttcg gcaacgccgt aatcgaagcg gcgcacaccc aaatagtcgt
    21901 gcagattttt ctcatttact ttaaccgccc tgggcttggc ttttccggtt tttttggttt
    21961 ttgagtcttc gtccaaggta atctgcatca ccaccttgcg gcagattttg gcaatctcgc
    22021 ggtcgagcga acgcacgccc gcctcgcggg tgtagtaacg gataatatcg cgcaccgcgc
    22081 tttcttcgat tgccaattcc ccttctttta cgccgttgcg cttcatttgc ttcggcacga
    22141 ggtactgcat cgcgatattg attttttcgt cttcggtata gccggacaga cggatgattt
    22201 ccatacggtc gagcagcggt gtcgggatat tcaggctgtt tgaagtcgca ataaacatca
    22261 catcgctcaa atcgtaatcc acttcggcat aatgatcggc gaacttgttg ttttgctcgg
    22321 gatcaagcac ttcgagcaac gcgctggcgg gatcgccccg gaagtcgcta cccaatttgt
    22381 cgatttcgtc gagcaggaac aatgggtttt ttacgccggc ttttgccata ttctgcaaaa
    22441 tcttaccggg catggagccg atataggtgc ggcggtgtcc ccggatttcg ctttcgtcgc
    22501 gcacgccgcc caaagccatg cggacatatt tccgccccgt tgctttggcg atggattcgc
    22561 ccaaagaggt tttgcccacg cctggagggc cgaccaggca cagaatcggg cctttgagtt
    22621 tgtccatacg tttttggacg gcgaggtatt ccaaaatccg ttctttgact ttttccaggc
    22681 cgtagtggtc ggtatccagc accaatccgg ctttggcgat gtctttgctg acgcgggatt
    22741 ttttcttcca cggcagttcg agcagggtgt cgatgtagtt gcgtacgacg gtggattcgg
    22801 cagacatcgg cggcatcatt ttgagttttt tcagttcgga caggcatttt tcttccgctt
    22861 ctttgctcat ccccgccttt ttgatgcctg cttccaaggc atccagttcg ccgttttcgt
    22921 cttcttcgcc caattctttg tgtatcgctt taatctgttc gttcagataa tattcgcgct
    22981 gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg tttttcggcc tgcataatgt
    23041 cgagttcgga ttccagctgt gccagcagga attccatccg tttgccgatt tcgggaattt
    23101 ccaaaatctg ttggcgttgc tccagtttca actgcaaatg cgctgcgacc gtatcggtta
    23161 gccggctgtt ttcggcaatg ccgttaatgc tgccgataat ttcggcgggg atttttttat
    23221 tgagtttggc gtattgttca aactgcgcca acagggtgcg gcgcacggct tcgaggtcgg
    23281 tattgccgcc cgtgtcttct tccacgaccg tctctatatg ggaaacgaac agaccgcccg
    23341 tgtcttcaat ggtcagaaca cgtccgcgat acagcccttc gaccaatact tttaccgtgc
    23401 cgtcgggcag tttcaacact tgcaggactt gtgcgaccgt accggtctga tacaggtcgg
    23461 cggcaaccgg ttcttctacc gccgcatcgg tttgcgccaa caggaaaacc ggctcctcgc
    23521 gggtaatggc gttttccagt gcggcgatgg atttcggcct gccgacaaac agcggcagaa
    23581 ccatatgcgg gtaaacgacg acatcccgca aaggaagggt tgccaaggcg gcatattcct
    23641 caaaatgctt ttctttttgt gtcataggta ctctcttgtg tctgacagat tgccgatttt
    23701 cgcgtacatt ggggttgaag gtattatttc aagcatatgt ggtttattta tggagtttga
    23761 tgcgatgccg tctgaaacat tccggcttca gacggcatgg gcttggaaag acaaggcggg
    23821 aacaaaaaac tgttctgtgt tgccgctcct tgctgtacca tccgtatggt ttgcggttct
    23881 gccgccctat tttcaaaaca tgacgcaggt atcccatgtc ttcttttatt tttcccgatg
    23941 acgggttcga atggcgcaac gaaagtctgc aaaagccgcc caaaggttgg cattatgtcc
    24001 gcgaaagcgg tgcaaacggc attttgaagg ctgcctatca aaatattgca actgtctggc
    24061 agggcgattt ccacaatgcc aaacaggtgc tttccgcaat gaagaagagg gttcgcaaac
    24121 ctgccgccgt ccgttccgat gcggatatcg ccgccctttt ccacgcccac cgtatgaagc
    24181 aggcgcagca gagccgtatt ctgaatatgc ttgccgttga aatccgcccc ggttttgtgt
    24241 tggacaacaa acgcgcgccc gatatacgcg ccgccctgct cgacgtgtac ggagaggcgg
    24301 acggcaaacc gtttttcctg ccgctcaatc tgctgctggg gtttatgggt gcgcacgagt
    24361 ggcataagaa aggggttgcc gttccgcagc tgggcggcag catacacgtt cctttcggcg
    24421 tattctcgcc gttgcgcggc gaatacctcg acctgctcgc ccatgcgccg tcaacgggtt
    24481 ttcagacggc attcgatatc gggacgggct cgggcgtgct tgccgccatt ttggcgaaac
    24541 agggcattcc ttccgtcatc ggcacggata ccaatccgaa agccgtcgcc tgcgcccgtg
    24601 ccaatattgc ccgtttgggc tttgaaaaac aggttgagat acgggaaacc gatctgtttc
    24661 ccgaagggtt tgccgatctg attgtctgca atccgccctg gcttcccgcc aagccgactt
    24721 ccgccgtcga atccgcgtta tacgaccccg aatctgcgat gctggctgcg tttttgcggg
    24781 atgcgccgaa acatctgaat cccgacggag aaatccgcct gatcatttcc gatcttgccg
    24841 aacatctgca cctgcgtccg tccgattttc tggataaggc atttgctcag gcgggtttgc
    24901 gcgttgcgga tgttttgaaa accaaaccgg ttcacaaaaa agctgccgat ccgaacaatc
    24961 cgctggcttt tgcccgaaac agggaaacta ctttcctgta ccgtttgaaa aaggcataag
    25021 gggcggcggt gcgcttccgg cggcaggcat cggaacgccc gcgccgccgg cacggcatca
    25081 ttcgggcgga ttattcttgt gaaaataccc gctcgagcat actgcccaat gccgtctgac
    25141 gcgttttgac ggtggcggca gcttgccgga aggcttcttc cccgccgaaa aatacgaact
    25201 tttccctcgc gcgggtaatg gctgtgtaaa gcagccctct gctcaagccc gaaagcgcgt
    25261 cgtccgcgtc gtccgaaccg ccggcggagg gcggcagcag ccaaacttcc cgatattccg
    25321 aaccctggct tttgtggacg gtcagcgcga aagcgggttc gaaatcgggc aggcagctta
    25381 ccgctacctt tttaaatccg tccgcatcgg caaaataggc ggcaaggctg ccctgccgtc
    25441 cgacatcttc cataatcagt ccgatgtcgc cgttgaacag ttcaagcgcg tagtcgttct
    25501 gtctgatcat aatcggttct ccggcgaaat aggcctgatg ttccggtatg ttcattttgc
    25561 ggcgtacatg gctgcaatag gcttcgttga agtcttccgc atcctgccgc cacgctgcca
    25621 ggaccacgat atccgagatg cctgcgtatg cggcttcgat attgccgtct tttaccgcct
    25681 gccaataggc tttgtgcgcc cggtacaacc tttcgactcg agcgttcgga ctgcattccg
    25741 aatgttccag ttcgtccgga aaccggtcaa acaatgccca cgccccctca tcgcccgata
    25801 tggcggcacg ggcaaggcag ccgatgccgc tgttgtcgcc gaagcggtgg ctgaacgaca
    25861 gatgggcggt gttttgcgcc agcacgggcg gatttgcgct gacgctgaaa ccgtgttccg
    25921 gaaggaagcc ggccagcctt tggtgcgttt ctccgtccaa aacggttttg tgcgacaaaa
    25981 cggacagcac cgccccgatt ccgacggacg ggagctggtt ttcatccccc agcagaatca
    26041 cgcgcgcgcc ggttttgacc gcttttaaaa gttgcaacat caatgcggta tccaacatgg
    26101 aggcttcatc gacaatcagc acatcgaacg gcagcgggta aacagggttg aacgccgcct
    26161 gcattttggg cgggcgcagc ttcagcagtc ggtggacggt ttgcccttcc agtttgagca
    26221 aatggcggcg gacggcctcc ggcgcgtcaa aaccgttgat tgcacggtgc agcgcgcgcg
    26281 ccatatgtgc cgccgctttg cccgtcggtg cggcaagcgc gatgttggga agattttcgt
    26341 cttcaccgca aatcagcgcc agcagtttgg caaccgttgt cgttttgccc gttcccggcc
    26401 cgccggtaat caccataaaa gactgcaaca gtgccaaggc ggcggcatcg cgctgccctt
    26461 cgctgcccgt gccttgaaac cattttgcga ggttttgccg cgcgcctgcc gcgtcggggg
    26521 cggatgtgcc ggctgccgcc aaacgtttta tttcggcagc caaatcgtat tccaactgcc
    26581 acatcctgcc caaaaacagt ttttcccctt ccaaaatcag aggggaggca gattccccga
    26641 ctacgggctt gagtgtcgaa aggaaggacg cctccccttt tttcaaacgg ataaacgaat
    26701 gaccgttctg caatgcctga aacaggcgtt cggtacagtt agcaagcact tcgctgcccg
    26761 aacccgcata gcgttccaaa aaacggattg ccgcccttgc cgccgcttgg gcaaattcat
    26821 ctgtctgccg ttccatcgta ttccttgtcg aaatgctgtc tgaaggcgcg aggcttcaga
    26881 cggcgcgtgt tgtttcggct gtttaggcat ttgcgccctg tttggggtgc aggctgccgt
    26941 ctttcatgac catcacgcgc tcgaagcggc cggcgagttc gtcgtcgtgc gttacgacca
    27001 ccagccccgt tcccaattct gttttcagtt ccagcatcat atccaaaaca ttcctggcgt
    27061 tcgcacggtc gagattgccg gtcggctcgt cggcaagcag gcatttgggt tgcgtaacca
    27121 aggcgcgcgc aatggcggca cgctggcgtt cgccgcctga aagttcgccc gcgcggtgcg
    27181 tcgaacggtg tttcagcccg accttttcca gcatcgccat tgccgtttct gctgcctctt
    27241 cacggctttt tttgccgatc agaagcggca tcatcacgtt ttccagtgcc gaaaattcag
    27301 gcagaagatg gtggaactgg tacacgaaac cgagatggcg gttacgtaaa tcgcccaaac
    27361 gccgctggtt taaggcacgc aaatcctcgc ccatcagcag caccctgcct tcagacggca
    27421 tatccagccc gcctagaata tgcagcagcg tcgattttcc gctgcccgaa gaaccgatga
    27481 tgccggtgct ttcccccgcg cggatttcca aatccaagcc gtgcagcacc cgaacgtcca
    27541 aaccgccgtc ccgatagcgt ttgcccacgc cttcgcattt caaaatcaac tcactcataa
    27601 cgcaaagcct ccgccggttg ggtttttgac gcgcgccggc tcgggtagag cgtggcaacg
    27661 aaagacagtc ccaaagaaat gcaggcaatc agggcaacgt cgcccatatc aacatcgctg
    27721 ggcaggtagt cgataaaata aacctgcgaa ttgataaggt ggacaccgag caggttttca
    27781 aaaaatgcca cgaccctgcc gacgttccaa cccaaaagca cgccgcagac cacacccgcc
    27841 agcgtgccga aaaagcctga aaacgcgccc tgcaccataa aaatcttcat cacgccggca
    27901 ggggaaagac ccaaagtccg caaaatcgca atgtccgcct gcttttccgt aaccgccatc
    27961 accaaggaag agacaaggtt gaacgccgcc acggcgataa tcagcgtcag gatgatgaac
    28021 atcatccgtt tttccaactc gaccgcctca aaatagctgc ggttgctgaa cgtccaatcg
    28081 cgtacccaaa ccgtatccct ttgcgcctcc ggaatcagct ttgccgtcaa ggcgggggcg
    28141 ttttgcgggt cggcgagttt cagccgcagc cccgcaactt ccttatccaa gcggtacagt
    28201 acgcgcgcgt cttggatatg cgtcatcgcc aatgagttgt ccacttcgta aacgcccgtc
    28261 ttaaccagac cgaccacggt aaactgcttc aggcgcggca cgactccggc aggggtaacg
    28321 ttgccctccg gcgtgatgac ggtaacttta ttgccgactt ccgcccccaa agcctccgcc
    28381 aagccgacac cgaggataat gtcaaactcg cccggaatca ggtcttcaaa cttgcctgcc
    28441 ggcattttgt cgccatattc caccactttg cgttcttcag acggtaaaat gccgcgcatc
    28501 tgcacgcccc tgatttcgcc cgcgtttgcc agcagagcct gattggaaac atagggcgcg
    28561 gcagccaaaa tacctttgcg gttttccgta aaccgaagca ggttgcgcca atccgtatcc
    28621 gtattgtcga tatagccgat ttcggcgtgc ggcgcgacat tcaggagctg cccgcgtatt
    28681 tctttctgaa agccgttcat aaccgacaag acgacaatca gcgcggttac gcccaaggcg
    28741 attccggcaa tcgaaaccat cgtgataaac gacataaagc cgttgcgctt tttcgccctg
    28801 agatacctca agcctatcca agcctctaga gaaaacataa cgctacctta aaaatgtctg
    28861 caaacgtgcc gccccggacg gcggtttggg ggcggcggca aaagtttatt gtaccgtaaa
    28921 accccggcag cgtccgaacg cccgagtgcg ggggacgggg cggcagagcg ggcggaaaac
    28981 ttgcacaacg cgtcaaactg ccctatcctt tccttcagaa aaaccgtttc ttgaggaaaa
    29041 caatgaatat ccgaactgct tttgctttgt gcgccatcgc cttatccgcc gctgcggctg
    29101 cctacgccaa agaaatcaaa atcgatgcca acaacacgcc ttattccgaa gccgacgcgc
    29161 aaaagctggc ggcaacggca gtcggtatgg gtgttaagga acctatcagc ctgaacggcg
    29221 gcagcgacag cattaccgtg tccggcagca gcgcgacgca gtgcgtgttc aaagtcggca
    29281 acggaggcgc attgcagatt caagggctaa actgcaagta aaccgcccgg aaaaatgccg
    29341 tctgaaggct tcagacggca ttttgcattg gcggcgttat gccccgcctt ctttaattag
    29401 gcggcgttcg tacaccgcct gcgccagcgt tcccgcatcg acatattcca attcgccgcc
    29461 caaggggatg ccctgcgaca gcctgctgac tttgtaaggc aggtttttaa aaaactcgga
    29521 caggacatac gccgtcgcat tgccttctgc ggtaaaagcg gttgcaataa tgatttcttc
    29581 gacttccccg ccgcccagcc gttgcgccag cctgtccaat gcgatggcgg atacgtccat
    29641 tcccagtgcc gtattgattt gccccatcag gacgaaatac agcccgtcgt ggcagtttgc
    29701 cgcttccata ttcgacacgt cggcaggcat atgcaccacc atcagccgcc gcccgtcgcg
    29761 tgtttcatcg gcacaaatat cgcacaatcc gccttcgcaa aacgtgttgc acatcgcgca
    29821 atggtaaacc tgcttcaatg ccgtctgcaa ggcatccacc agtttttcag cctctttgcg
    29881 cttgtgttgg agcaaatgat acgctatccg ctgtgccgat ttcggcccga cgttgggtaa
    29941 aaccttcagc gcgtcgatca atccttggaa ggcatcttgt tttttgtggc tcatcatatt
    30001 ccgccgtatg ggaaaacggc cggaatattc cgaccgttat tttgtcaaca aaagtgtcaa
    30061 ttactgaccg tcgccgttgt cgaccgattg cgctcctttg gtctgtttga ttttgccgtt
    30121 gaaataacgt atcaacaagt cgaaagtatt ggcagactgc tgttgcgcca aagcctgttt
    30181 tgcaagcgga agctgtgcgg cgatatcatc cggcggggtt acagcctgta cttcgacaat
    30241 cacgggtgcc ggcagaccga tcagcctgac gtaggcgggt ttgccgtttg ccggttttgc
    30301 tttcagcagt tccgcataag cctcgggcgg catggactgc cttgcctgct gtgcgcccaa
    30361 aacggacact tccgaccatt tcacgtcaac agccttgccg ccgttcagtt gggtaagcac
    30421 gtcttttgcc ttgttttcgg caagtttggc ggcttcggta cggatataag cctgacgtac
    30481 cgcgtctttg gcttcggcaa acggcagggt tttctcttcg cggacttctt tggcgcggac
    30541 gacccacgcg gtttcgctgt tgatggtcag cacttcggaa ttgtgttttt tcttcaatac
    30601 gtcgtcgctg aatacggcat tgatcaggtt ttcgggcata ccggacattt gcgcatcctg
    30661 cctgctcagc caagtttctt gggtttcgac tttcaaaccg ctgtttttgg cggcttcggc
    30721 aagcgaggaa ggatggttga acgcgtcatc gcccagcttt tcttttgcct tattgaagtc
    30781 ggcaaccgcc tttttcattt tcaattcgtt ttcgacggcg gctttttcct gctcgaaaga
    30841 aggtttggct tcatttgccg gcaaacgcgc cacgcgctct tcaaacgcat ttttcacttc
    30901 tgtttcgctg acggtctgtt tgtctgcaaa atccttcaga ttcaaggcga catattccaa
    30961 tttgaccgct ttgggaagca ggtagtcttt tttgtttgcg ttataaaact tctgcaaatc
    31021 ggcttcagac actttgactt gggcgatgaa ttcgtcgggg ttgaaagtgt gcgaacggat
    31081 ggtgcggttg acctgcgtca gcctgatcag ctgttccgcc tgcgcgtcgc cgaccaatac
    31141 gccgttttgg acgaggttta ccaaattctg caaggcaaac tgatcgcgga tttcttcgac
    31201 aaactggtct tcagacatat ggcgttggga aaggtagcgg tttaaaagcg cgtggtcgaa
    31261 tttgccgttt gcgtcgtgga aattgggatc gtccacgata atctgcttga tttgttcgga
    31321 agaaaccgaa atgcccatca gcttcgcgcc ctgtttcagg taggcgcgtt gcagcaggga
    31381 ttggaacacc gcgtcgcgcg aagggctgcc gccgcccgcc tgttcgttct gtatggcgtt
    31441 gttgatggag tggtcgctga ttttttcgtc gcccacttgg acgatgtagt cggcacccgg
    31501 atgggatacc gtgctgaccc cgaagccgac gaaggttaat gcaatcaggc ccaaaaggac
    31561 ttgggcgggc gttctgtatt tttcgatgga atggaacata ttttaaatcg ggatatagaa
    31621 tgggaacggg aaattcaagt cgggtattgt aacggttttt atccctgtct gcacggggct
    31681 tgccgcttga agatgccgtc gtaggtttct tcgctgaagc cggcgtaaac cttgccgccg
    31741 cactccaata cgggacgctt gatcaggctc ggcatttcgg acatcagttt gacggcctcc
    31801 gccgtcgagg acagcacttt ttgctgtgtt tcggcatcga gtttgcgcca gcttgtcccg
    31861 cgtttgttga gcagggttgc caaaggcact tgttccagcc acgagcagat ttccgcttca
    31921 gacggcctct gttttttgaa atcccgaaat tcaaattcca taccatatcc ggcaagtcgg
    31981 tttttggctt ttttgaccgt atcgcaattt gggatgccgt gaaggactat catttggaaa
    32041 ccttttgtct gaaataataa aacggatatt ttactataag tgtctgaaaa tttgcccgtc
    32101 tgtttcagac ggcggggcgg ttatgttaca atccgaaaat tcgaaaaatt taatctcttg
    32161 ttcaataaag gctttaccaa tcatgatttc taccaacggc atcaccatgc agttcggcgc
    32221 gaagccgctg tttgaaaacg tatccgttaa attcggcgaa ggcaaccgct acggtttgat
    32281 cggcgcgaac ggctcaggca aatccacctt catgaaaatc ctcggcggcg atttggaaca
    32341 gacggccggc gaagtggcga ttgaaaacgg cgtgcgtttg ggtaaattgc gccaagacca
    32401 gtttgcctac gaagacatgc gcgtgctgga cgtggtgatg atggggcata ccgaaatgtg
    32461 ggcggcgatg accgaacgtg atgcgattta cgccaatccc gaagccaccg aagacgacta
    32521 catgaaagcc gccgaactgg aagccaagtt cgccgaatac gacggctaca ccgccgaagc
    32581 gcgtgccgcc gaactgttga gcggcgtggg catttccgaa gatttgcaca atgcgaaaat
    32641 ggcggaagtc gccccgggct tcaaactgcg cgtattgctg gcgcaggcgc tgttctccaa
    32701 gccggatgta ttgctcttgg acgaaccgac caataacttg gacattaata ccatccgctg
    32761 gttggaaggc gtgttgaacc aatacgactc cacgatgatt atcatcagcc acgaccgcca
    32821 ctttttgaac gaagtctgca cgcatatggc ggatttggac tacaacacca tcaccatcta
    32881 tccgggcaac tacgacgact acatgctcgc ctccgcccaa tcgcgcgaac gcgccctgaa
    32941 agacaatgcc aaagccaaag agaaactgca agagctgcaa gagttcgtcg cccgcttctc
    33001 tgccaacaaa tccaaagccc gtcaggcaac cagccgtctg aaacaggccg acaaaatcaa
    33061 atcggagatg gtcgaagtca aaccttccac ccgtcaaaac ccgtatatcc gttttgaagc
    33121 cgatgaaaaa gccaagctgc accgtcaggc tgtggaagtt gaaaaactgg cgaaacgctt
    33181 tgaaccccag ttgtttaaaa acctgaactt tatcctcgaa gcgggacaac gccttgccat
    33241 catcggcccg aacggcgcag gcaaatccac cctgctgaaa ctcttggccg gcgcgtacaa
    33301 ccccgaatat tcagacggcc tcttgccgga cgaaggcagc atcaaatggg cggaaaaagc
    33361 cagtgtcggc tactatccgc aagatcatga aaacgacttc gacgtcgata tggacctgag
    33421 cgaatggatg cgccaatggg ggcaggaagg cgacgacgaa caagtcatcc gcggcacttt
    33481 ggggcgtttg ctcttcggca gtaacgatgt cgtgaaaaaa gtgaaggttc tctccggtgg
    33541 tgaaaaaggc cgtatgcttt acggcaaact gttgctgttg aaacccaatg tcttagtcat
    33601 ggacgaaccg accaaccata tggacatgga aagcatcgaa tccttgaaca tggcgctgga
    33661 aaaatacaac ggcacgctga tttttgtctc ccacgaccgt cagttcgttt cctccttggc
    33721 aacccaaatc atcgaactgg acggcaaagg cggatatgaa cactacttgg gcgattacga
    33781 aagttacttg gagaaaaaag gcgtagcata accgccggtt ggaacaatgc cgtctgaagc
    33841 cgcttcagac ggcattgttg ataactttaa aataggaagc atatgcagac ttacctcgtc
    33901 ggcggcgccg tccgcgatta tcttttgggc ttgcccgtca aagaccgcga ttgggtggtc
    33961 gtcggcgcag acgcacaaac catgctggcg caaggcttcc agccggtcgg caaagatttt
    34021 cccgtgtttc tccatcccga aacgcacgaa gaatacgccc tcgcccgcac cgagcgcaaa
    34081 accgccaaag gttacgtcgg tttcagtttc cacgccgaca aagacgttac gctggagcag
    34141 gatttgatgc gccgcgacct gaccatcaac gcgatggcgc aagatgcgga cggcaagatt
    34201 atcgaccctt tcggcggaca acgggatttg gcggcaggca ttttgcgcca cgtttccccg
    34261 gccttcgccg aagaccccgt ccgcatcctg cgtactgccc gctttgccgc gcgttacagg
    34321 tttgaaatcg ccgaagaaac cataaagctg atgcggcaga tggtggaaaa cggcgaagcg
    34381 gacgcattgg ttgccgaacg cgtctggcag gagttggcga aaggtttgat ggaaaaaaat
    34441 ccgcgcaaaa tgattgaagt gttgcgcgaa tgcggcgcgc tcaaagtctt gctgcccgaa
    34501 gtcaatgccc tcttcggcgt gccgcaacgc gccgactacc atcccgaaat cgacagcggc
    34561 atccataccc tgatgacgct gcaacgcgcc gccgatatgg gcttgagcct gcccgaacgc
    34621 tatgccgccc tgctgcacga cttgggcaaa gccaaaacac cgtccgacat cctgccgcgc
    34681 caccacggac acgacctcgc cggtgtcgaa cccatgcgcg aagtcaatca gcggctgcgc
    34741 gcgccgaaac attgcgccga gcttgccgaa ttggtttgcc gttggcacat tattttccac
    34801 caagtcggac agcttaaaag ccaaaccatt ctgaacgttt tgaaaaaaac cgacgctttc
    34861 agacgacccg aacgctttca gacggcattg aacgtctgca ttgccgacac gcaaggccgt
    34921 ctgaaccgcg aacacacgcc ctacccgcaa cgcgcgcact ggctcgcctt actcgaagcc
    34981 gccaatcagg cggattcggg caaaatcgcc gccgaatgcc gcgcgcaggg aaaagcgcac
    35041 cttatcgccg aacaaatcga ccgggcgcgg ctggcacaaa tcgccccatt gcaaaaagcg
    35101 tttcgagcgg cgcaagacaa aacagaaaaa cattaaaacg tccaatgcag cca
//
LOCUS       DAWKQJ010000005        29776 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000005 DAWKQJ010000000
VERSION     DAWKQJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.005"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(49..1230)
                     /gene="odhB"
                     /locus_tag="WJL20_000173"
     CDS             complement(49..1230)
                     /gene="odhB"
                     /locus_tag="WJL20_000173"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ7682384.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1330..4158)
                     /locus_tag="WJL20_000174"
     CDS             complement(1330..4158)
                     /locus_tag="WJL20_000174"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ7682385.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4363..5646)
                     /gene="gltA"
                     /locus_tag="WJL20_000175"
     CDS             complement(4363..5646)
                     /gene="gltA"
                     /locus_tag="WJL20_000175"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7682386.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5758..6006)
                     /locus_tag="WJL20_000176"
     CDS             complement(5758..6006)
                     /locus_tag="WJL20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ7682387.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6010..6717)
                     /locus_tag="WJL20_000177"
     CDS             complement(6010..6717)
                     /locus_tag="WJL20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ7682388.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6838..8601)
                     /gene="sdhA"
                     /locus_tag="WJL20_000178"
     CDS             complement(6838..8601)
                     /gene="sdhA"
                     /locus_tag="WJL20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ7682389.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8604..8945)
                     /gene="sdhD"
                     /locus_tag="WJL20_000179"
     CDS             complement(8604..8945)
                     /gene="sdhD"
                     /locus_tag="WJL20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ7682390.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFALPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8939..9316)
                     /gene="sdhC"
                     /locus_tag="WJL20_000180"
     CDS             complement(8939..9316)
                     /gene="sdhC"
                     /locus_tag="WJL20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ7682391.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9595..10998)
                     /locus_tag="WJL20_000181"
     CDS             complement(9595..10998)
                     /locus_tag="WJL20_000181"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7682392.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPAGKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11247..11984)
                     /locus_tag="WJL20_000182"
     CDS             complement(11247..11984)
                     /locus_tag="WJL20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ7682393.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIHMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12263..14539)
                     /gene="metE"
                     /locus_tag="WJL20_000183"
     CDS             complement(12263..14539)
                     /gene="metE"
                     /locus_tag="WJL20_000183"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ7682394.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLNLLPKLLPVYVEILNALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAAGSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSTGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14675..15553)
                     /gene="metF"
                     /locus_tag="WJL20_000184"
     CDS             complement(14675..15553)
                     /gene="metF"
                     /locus_tag="WJL20_000184"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ7682395.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15754..16029
                     /locus_tag="WJL20_000185"
     CDS             15754..16029
                     /locus_tag="WJL20_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ7682396.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16029..16154
                     /gene="ykgO"
                     /locus_tag="WJL20_000186"
     CDS             16029..16154
                     /gene="ykgO"
                     /locus_tag="WJL20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ7682397.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16260..16460)
                     /locus_tag="WJL20_000187"
     CDS             complement(16260..16460)
                     /locus_tag="WJL20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682398.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16603..17742
                     /locus_tag="WJL20_000188"
     CDS             16603..17742
                     /locus_tag="WJL20_000188"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ7682399.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17739..18320
                     /gene="metW"
                     /locus_tag="WJL20_000189"
     CDS             17739..18320
                     /gene="metW"
                     /locus_tag="WJL20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ7682400.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18437..19282
                     /locus_tag="WJL20_000190"
     CDS             18437..19282
                     /locus_tag="WJL20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ7682401.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19332..19892)
                     /gene="efp"
                     /locus_tag="WJL20_000191"
     CDS             complement(19332..19892)
                     /gene="efp"
                     /locus_tag="WJL20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ7682402.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19935..21083)
                     /gene="earP"
                     /locus_tag="WJL20_000192"
     CDS             complement(19935..21083)
                     /gene="earP"
                     /locus_tag="WJL20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ7682403.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRFARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLNI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLDMWQQAGSALTLLLAGAQIIDS
                     LKQSGVIPQDALQNDGDVFQTTSVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21102..21380)
                     /locus_tag="WJL20_000193"
     CDS             complement(21102..21380)
                     /locus_tag="WJL20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682404.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMFLFIPIVLLIVYLFHYFGAF"
     gene            21773..22714
                     /gene="miaA"
                     /locus_tag="WJL20_000194"
     CDS             21773..22714
                     /gene="miaA"
                     /locus_tag="WJL20_000194"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ7682405.1"
                     /translation="MPIPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAECSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFHALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPEDRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            22718..23437
                     /locus_tag="WJL20_000195"
     CDS             22718..23437
                     /locus_tag="WJL20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ7682406.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            23442..23930
                     /locus_tag="WJL20_000196"
     CDS             23442..23930
                     /locus_tag="WJL20_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ7682407.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            23996..24748
                     /gene="rlmB"
                     /locus_tag="WJL20_000197"
     CDS             23996..24748
                     /gene="rlmB"
                     /locus_tag="WJL20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ7682408.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(24810..26201)
                     /locus_tag="WJL20_000198"
     CDS             complement(24810..26201)
                     /locus_tag="WJL20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ7682409.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            26508..27383
                     /locus_tag="WJL20_000199"
     CDS             26508..27383
                     /locus_tag="WJL20_000199"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ7682410.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPESFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            27392..28588
                     /gene="bamC"
                     /locus_tag="WJL20_000200"
     CDS             27392..28588
                     /gene="bamC"
                     /locus_tag="WJL20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ7682411.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTFPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(28645..29577)
                     /gene="pip"
                     /locus_tag="WJL20_000201"
     CDS             complement(28645..29577)
                     /gene="pip"
                     /locus_tag="WJL20_000201"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ7682412.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         7448 a         8138 c         7369 g         6821 t
ORIGIN      
        1 aaattcagac ggccttttca ttaacaaata aaaggccgtc tgaaacgatt acagatccaa
       61 caacaggcgg gctgggtctt ccaacgcgtc tttaatggct accaaggtca atacagcttc
      121 gcggccgtca atgatacggt ggtcgtaaga cagagccaga tacatcattg gacggacaac
      181 aacttggccg ttttcaacca cagcgcgctc tttagtggcg tgcataccca aaatcgcaga
      241 ttgaggcgga ttgatgatcg gggtggacat catagaaccg aaagtaccgc cgttggtaat
      301 actgaatgta ccgccggtca gatcttcgat agcgattttg ccgtctttgg cttttttcgc
      361 gtaatcaaca attgcttgtt cgatgtcggc aatgctcatt tggtcggcat cgcgcaggat
      421 tggcacaacc aaaccgcgtg ggctgccaat cgcgataccg atgtcgaagt agccgtggta
      481 cacgatgtct ttgccgtcaa cagaagcatt cacaaccggg tattttttca gggctgcaac
      541 agcggcttta acgaagaagg acataaagcc cagtttcacg ccgtgttctt tctcgaattt
      601 ttctttgtac ttcgcacgca agtccatgat tggtttcatg ttgacttcgt tgaatgtagt
      661 cagaatggcg ttttcttgtt gagaagccaa gaggcgttct gcaacacggg cacgcaggcg
      721 gctcatcggt acgcgttctt caggacgtgc gccggcagga agtgcaacgg caggagcagc
      781 ggctgcggca ggtttggctg cggcattttg tacgtcttct ttcaatacgc gaccgtcacg
      841 gccggaacct tgcaatgcgt tcacgtcaac accgctctct gcagccagtt tggcggcggc
      901 aggcatagcg gcgttgtttt gtgcagcagc aggagcggcg gccggggcag cttcggcagg
      961 agcggcggca ggtgcttcag cagcggcagt agcagcggta tcgatacgtg ccaaaacttg
     1021 gtcggcaaca acggtttcac cgtcttgcgc tacgatttca accaatacgc cggcttgtgg
     1081 agaaggtact tccaaaacca ctttgtccgt ttcgatatcg atcaggattt cgtcacgggc
     1141 aactgcttcg ccaacttttt tcttccattc caagagcgtg ccttcagata cgctttcaga
     1201 cagcataggt acttttacat caataatcat tttgtgtctc caatggccct ttcagacggc
     1261 ctgttgtgtg tttttcattc tgcatatggg tgttccgcct gagcagcctc agacggaatg
     1321 ctacttggct tacaatgcca aagcgtcttc aaccaattgt ttcaattgag caatgtgttt
     1381 gcttgagtag cccactgcag gcgatgcgct gcttggacga ccggcataag acagtttttg
     1441 ctcttcgctg ataacgtctt cgatgcggtg gcggatttgg tagaacgcgc cttggttttt
     1501 cggctcttct tgtgcccaaa ccacagattt tgcgttcgga tatttcgcca gttcggcttt
     1561 aacctcgtcg tatgggaacg gatacagctg ctcaacgcgg acaatagcga catcatcttc
     1621 cagtttacgt tcggctcgac cggcttccaa gtcatagtaa acctgaccgg cacacaatac
     1681 cacgcgtttc acgctgtcgt tgcttgcgcg ttcggcggta tcgccgataa ccggacggaa
     1741 agtcgaacct tcggtgaagt tttccagcgg gctcattgcc cctttgaatc gcaacaggcg
     1801 tttggacatg aaaatcacta gcggtttgcg gtatgaacct aagacttgac gttgcaagag
     1861 gtggaacatt tgcgacgctt cagacggcat gatgacttgc atattgttct cagaacacag
     1921 ttgcaaccaa cgttctacgc gtgcagaaga gtgctcggga ccttgaccgt cgtagccgtg
     1981 cggaaggatg gtggtcagac cacacaaacg accccatttg gtttcgcctg aagacaggaa
     2041 ttggtcaata gtcacttgcg cgccgttggc gaagtcacca aattgagctt cccaaatggt
     2101 cagcttgtca ggcgcggagc aggcaaagcc gtactcgaac gccatcacgg cttcttcgtt
     2161 caaaatggag tcgataacca agaactcgcc cataccttcg cccatatggc gcagaggaac
     2221 ataagtaccg tcatcccatt tttcacgttt ttgatcgtgc aatactgcat gacggtgtga
     2281 gaatgtaccg cgcccggagt cctcaccgga gatacgcacg ccatgacctt tggttacaag
     2341 gcttgcgtat gccaaggttt cggccatacc ccagtcgatg gcttgtttgc cggatgccat
     2401 ggctttacgc gcttcaatca cacgttttgc agtcggatgc agggcaaagc cttccggtac
     2461 ggcggtaaat ttctcggtaa gacgctcaat atccgcggca ggcaaaccgg tttcgatgtg
     2521 ttcgcgccaa tctttacctt ggtatttgct ccagtcaatt tgtgtgcgtt ggaagttgct
     2581 caacgttgtt tgctcgacgt gttcgccttt atccaaagca tcacggtagg cttggatgta
     2641 accgtcagcc tcggcttggg ttaccacgcc ttcggcaatc agttgctcgg tgtacaaagc
     2701 acgcgcaccc gggtgttgcg atactttttt gtacatcatc ggttgggtca aggtcggatc
     2761 gtcgccctcg ttgtggcccc atttacggta gcagacaacg tcgatcacga tgtctttatg
     2821 gaattttttg cggtaatcca aagcggcttg gatggcaaag caaacacgct caggatcatc
     2881 gccgttcaca tggataaccg gagcggaaac catttttgcg atatcggtac agtgtacggt
     2941 tgaacgggta tcgcggatat cggaagtggt aaagccgatt tgattgttga tgacgatatg
     3001 aaccgtaccg ccggtggtat aaccgcgcgt tttagacaga ttgaatgtcg cttggttgac
     3061 acccaaaccg ataaatgcgg agtcgccgtg aatcaatacg ggcaagactt tatcgcggcc
     3121 gttttcgccc aaacgtttct gcttggcgcg tgcagaacct tcgactaccg ggttaacgat
     3181 ttccaagtgt gacgggttga acgccaaaga aacgtgcatc ggaccatgtg gagtggcgat
     3241 gtcggagctg aagcccatgt ggtatttcac gtcgccgcta ggcaatttga tttcggcacg
     3301 gccttcaaac tcagcgaaca aatctccggg ttttttaccc aaaatgttca ccaaaacatt
     3361 cagacggcca cggtgcgcca taccgatgat gacttcttcc acgccgtctt taccggcgtt
     3421 ttgaatcagg tagttcaaac cggcaatcgc gctttcgccg ccttcgacac cgaaacgttt
     3481 ctgaccgaca tatttggtat gcagataacg ttccaaagtt tcggcagcag tcatctcttt
     3541 caagatacgg cgtttttgat cggcattgta atgcggtgtg gacaatacgc tttcaaaata
     3601 attgcgtacc cagcggcgct cttcggtatt gggaatatag atatattcca atgcgatgtg
     3661 gccgcagtag gtttgtttga ggttgctgat gatttgggac aaaggcagtt tgccgcgatt
     3721 ggcaaaatcg ccctcgccca tattgaattg aagcgccata tcggcatctg acagaccgtg
     3781 gaatttcgga tcgagggctt caatatcgcg cggggggata cgtttgagcg gatcaagttg
     3841 ggctgcaccc acgccttgga tacgataggc ggaaatcagc cgtaaaacgc tgacttgctt
     3901 tttcagcatt gcctcatccg caccgcccgc aacggcagat gcaattttct ttttcgccaa
     3961 agtaacaaat gattcgcgaa tcggtgtgtg tgcgacatcg acagcaaccg tccccggctg
     4021 tttgctcaaa tcggtgaaat actgcttcca tttttcatca accgcatcgg ggttttccaa
     4081 aaaagcctcg tacaattcct caatgtaggg tgcgttcgaa ccgaacaggt aagagaaatt
     4141 gagtttttcg tccatcatgg cgtgcgctct tttctttaaa atataaacaa agggtagaag
     4201 tccgcgtcct gacggctaaa ctgatgccgt ctgaaaagac attttattct accctatttt
     4261 aaaccggatt gcttgtcttg atttcatccg actacaatca gacggattcg ggaaacaatc
     4321 cggttctctg atattgcctg tttgacaata tattaatgta tgttacctct cgcctggcgg
     4381 cacataatcg cggcgttccg aaccggtata aagctggcgc gggcggccga ttttcagcga
     4441 aggatcgcta atcatctcgt gccagtgcga aatccagccc acgctgcgcg acagggcgaa
     4501 gatgacggta aacatttcgg tcgggatgcc cagcgcggac aggacgatgc cggaatagaa
     4561 atcgacgttt ggatacagtt tgcgttcgat aaagaacggg tctttcagcg caatctgttc
     4621 caattccatc gccagtttga atttcggact gtcttccaag cccaattcct tcaaaacttc
     4681 atagcaggtt tcgcgcataa tgctggcacg cggatccata ttgcggtaca cgcggtgacc
     4741 gaagcccatc agacggtatt tgcgttgttt cacaccttcc atgtatgcgg caacattaga
     4801 cacatcgccg atttcgtcca acattttcaa cacggcttcg ttcgcaccgc cgtgcgaagc
     4861 accccacagg caggcgatac cggcagcaat acaggcaaac ggattcgcac ccgaagagcc
     4921 tgccagacgg acggttgaag ttgaggcgtt ttgctcgtgg tcggcatgca aaataaagat
     4981 gcggtcgagc gcgcgtgcca aaacgggatt gggtttgtag tcttcacacg gcgtggcgaa
     5041 catcatatga aggaagtttt cggaataaga aagattattc ttcggataat tgaacggcag
     5101 accgtttgaa tagcggtagc acattgccgc aatggtcggg attttagaaa tcaggcggta
     5161 aatcgcgatt ttgcggtgtt cgggattgct aatgtccaag ctgtcttggt agaacgcaga
     5221 cagtgcgccg accacgccga ccatcatcgc catcggatgc gcgtcgcggc ggaagccccg
     5281 gaagaaccaa gtcagctgtt catgcaccat cgtgtggcgg cggacggtat tgtcaaattc
     5341 tgccttttgc tcgggagtcg gcagttcgcc gtaaatcaac aggtagcaga cttccaaata
     5401 atcggacttt tcggccagct gctcgatggg gtatccgcga taataaagca agccttgatc
     5461 gccgtcgatg taagtaattt tagactcaca gcttgcggtt gaaacaaatc cggggtcgaa
     5521 ggaaaacaaa cctgtatttt ttgtcagccc ccgaatgtca accacatcgt gcccgatgct
     5581 ggcttccaat accggcagct ccaaacctgc ctgacccggt acgttgagtt tgattgattt
     5641 ggacatatta ttgctccttt gcaagttata acccacgtta ctgcatccgc agcacgttct
     5701 ttgccttcag acggcttttg cattttgaaa tctgcctgca ggcagatttc agatgcctca
     5761 tgcccgtctg attttttcaa gcatcgggat aaggtgccct ttgtccgttt ccgaatgccc
     5821 gttaatcaag gcaagcaatt cttgatcttg aaattcaagg atttcggaaa actcggacag
     5881 ctctttatcg ctcaaatgct cgaattcttt ttccataaac ctgccgaaga ttaaatctaa
     5941 ctccaacaat ccccggcggg tttgaaaacg gattttccgt ttggcaatat cgtcaaaaac
     6001 catcatttct taaacggccc gtttcaacat aatctcttta atcttaccga tggctcgggt
     6061 cggattcaag tgtttgggac atacgtctac gcagttcata atggtgtggc aacggaacaa
     6121 acggtatggg tcgttcagat tatccaaacg ctcattagtg atggtatctc ggctgtccgc
     6181 aatgaagcgg taagcattca gcaaaccgga cggaccaacg aatttatcag ggttccacca
     6241 aaatgacggg caggcagtcg aacagcaggc gcacaaaata cactcgtaca aaccgtccaa
     6301 ctctttacgc tcttcctgag tttgcagacg ctctttgtcc gcatcaatcg gattatcgtt
     6361 gacaacataa ggtttgacgg aatggtattg tttgaagaac tgggtcatat ccacaatcag
     6421 gtcacggata acaggcagac ctggcagagg acgaatttta accggctgtt tcaagccacg
     6481 cagatcggtc aaacacgcca agccgttttt gccgttgatg ttcataccgt cagaaccgca
     6541 aatgccttcg cggcaggagc ggcggaaaga caaggtatcg tcttgtgctt tcaggcgtac
     6601 caaagcatcc aaaagtttca cgtcggtcgg ttccaattcc aactcgtaac gctgcatata
     6661 aggcttggca tcaacatccg ggttgtaacg gtaaatttca aaactcattt tttccatgag
     6721 aagtgttcct tttctcatca actgcccaat gattttgtat tataaattca agcagttcaa
     6781 tgtgggtatg gttagatttc agacgagctt atgaggcgaa aaccgtctga aaacgcatca
     6841 ataaacgcgc ttggccggtt tgatgtattc cacgctcaaa ggcttggtat gtaccggttt
     6901 gtaagacaaa gtattggtat ctgaatggta cagcgtatgt ttcatccagt tttcatcatc
     6961 gcgctcagga tggtcgtctg aagcgtgcgc accgcgtgat tctttacgtg cttcggcaga
     7021 caccaaagtc gctttcgcca cttcaattag gttatccaat tccaaagcct cgatacgcgc
     7081 ggtattccac actttgctct tgtctttgat ttcggtacgt ttcacacgct cggcaatcgc
     7141 catgacttct cgaacgcctt tgctcagaat ctcatcagta cggaacacgc cggcgtgcag
     7201 ttgtacggag cgttgcaatt cgcgacgcaa tgcatcaacg ttttcgccat cagtttgatt
     7261 gtccaaacgc tcgatacgtt ggcgggtcag ctcgcccgca tccgcaggca aaggtttcca
     7321 gtcgctttgc tctttgatga atttaatcat gctgtcgccg gcagctttac cgaataccac
     7381 caagtccaac agggagttgg tacccaagcg gttcgcaccg tgtacggaag cacaagcgca
     7441 ctcacctgcc gcatacagac cttttacagg cacttcgtat tcgtcgcctt gaggaacgac
     7501 aacttcgcca tggtagttgg tcggaatacc gcccatcata tagtgggtag tcggcacaac
     7561 gggaatcggg tctttaatcg gatcgatacc ggcgaactga atggaaatct cgcggatgcc
     7621 cggcagtttt tccataattt tttctgcgcc gatatggtcg attttcagta agacatggtc
     7681 tttgttttta ccgcagccgc gaccttcgta gatttccatc gccatcgcgc gggaaacaac
     7741 gtcgcgagaa gccaagtctt ttacggtcgg cgcatagcgt tccataaagc gttcgccgtc
     7801 ggcattcaac agaataccgc cctcgccgcg tacgccttcg gtaatcaaca cgcccgcacc
     7861 tgccacgccg gtcgggtgga attgccagaa ttccatgtct tccaacggga tacctgcacg
     7921 cgcacaaata cccaaaccat cgccggtatt catataggca ttggtagaag acgcataaat
     7981 acggccgccg ccgccggtag caaacatcac agctttagcg tggaaaatat aaacttcgcc
     8041 ggtttccatt tccatggcgg ttacgccgac gacatcgccg ttttcatcac gaatcaaatc
     8101 ttgtgccgtc cattccacaa agaattgcgt attggcacgg acattttgtt ggtacaaagt
     8161 atgcagcatc gcatgacctg tacggtcggc aaccgcacag gcgcgttcta ccgcgcgttt
     8221 accgtgttcg gcagtatggc cgccgaaagg acgctgataa attttaccgc tttccacacg
     8281 gtcaaaaggc atacccatgt gttccaactc aattacggct tcaggcgcgg cgcggcacat
     8341 aaactcaatc gcatcttggt cgcccaacca gtcggaacct ttcacggtat cgtacatgtg
     8401 ccagtcccaa cggtcttcct gcacattacc cagagaggcg gaaataccgc cctgcgccgc
     8461 tacggtatgc gaacgggtcg ggaacacttt agacaaaacg gcacaattca gaccggattt
     8521 ggataattgg agggctgcgc gtaaacctgc accaccaccg ccgacaatca cggcatcaaa
     8581 cttgcgaaca ggaaaaccca tacttacccc caaattactt taattgaata caccaagcag
     8641 ccgaccagcc agacgatggt ggcaacctgc aaaaacaaac gcacgccgaa gggtttgata
     8701 tagtccatcc acaaatcgcg gatacccacc caagcgtgca agaatacggc gatgaagctc
     8761 acttgggtaa atacttttac ccaagtttga ctaaaaaatg cctgccatgc cgaatattct
     8821 ttaggcagag caaatagaac cactaaaagt gcaacggtat aaatcaacat aataaccgcg
     8881 gtcgcacgtt gcatcgccca atcccgcaaa ccgtaatggg caccggtcaa tttacgttct
     8941 accataacaa cgctcccaaa acgacagtca aaaccaatgc agaagcaaat acggctttag
     9001 cggtattgcg cgcagtattc agctcaaggc ctttgtgcgc atccaaaaat aaaaagcgga
     9061 taccggcgag agaatggtgc agataagccc acaatacacc gattaaaacc agcttgacca
     9121 aaggatggga aacaatggca cggtaagttt caaatgcaga ctcttgactc agggtaccgg
     9181 acaggaaata cagcaggaaa ggcagcataa taaacagccc gaccccgctg atgcggtgaa
     9241 ggatggaaac tatcccgggt atcggcagac ggatgttcgg caaatccaga taaacaggac
     9301 gcggtttggc agacatagtc aattcctctg tagttatata tcgactcaaa aaccgtaaac
     9361 ccattgctac actgggttac ataaacgtta atttacctgt tttatcccat tttgaacagt
     9421 gtattttaaa aacaaactga tagattttga caaacacgcg tggaaacagc agattaaaag
     9481 aaagattttt agttttgccg cccccttccc gacacggtat cagcatccga ccccatcccc
     9541 aaaaacaaaa tgccgtctga aaaaatttca gacggcattg ttttgccata tcggtcaggc
     9601 tttcaatttc gcatcggcat cgcgcaacgc ggtacgcaga ccttcctcga taacgggatg
     9661 gtagaacggc atatccagca tttgcggaac ggtcatcttc atttgatgcg cccatgccaa
     9721 caagtgcgcc aaatgttcgg cggcagggcc tacgatttcc gcgccgataa agcggccggt
     9781 ggctttttcg gcatacaggc gcatatggcc tttgttcacc agcatcacgc ggctgcggcc
     9841 ttggtttttg aacgatactt cgccgatgac aaattcgtcg gcttggtatt gcgcggcaac
     9901 ctgcgcgtat ttcagaccga caaagccgat ttgcggactg gtaaacacca cgccgatggt
     9961 gctgcgccgc aaaccgccgc cgatattcgg gtaacggcct gcattgtcgc cggcaatctt
    10021 gccttggtcg gcagcttcgt gcagcagggg cagttggttg gacgcatcgc ctgcgatgaa
    10081 gatatgcgga atactggtct gcatggtcag cgggtcggca acaggtacgc cgcgcgcatc
    10141 tttttcgata ttgatatttt ccaaaccgat attgtcaacg ttcggacggc ggcccacggc
    10201 tgccagcata tattcggcaa caaatacgcc tttttcgcca tcctgctccc aatggacttc
    10261 tacattgccg tctgcatcga gtttgacctc ggttttagca tccagatgca gtttcaattc
    10321 ttcgccgaac acggctttcg cctcgtctga aacgacgggg tcggaaatgc cgccgatgat
    10381 tccgcccaaa ccgaaaattt caactttcac gcccaaacgg tgcaatgcct gacccagttc
    10441 caaaccgata acacccggcc cgaacacggc aacgcgctta ggcagcgtat cccatgagaa
    10501 aacgtcatcg ttgataatca aacgattgcc caaagactgc cattgcggca ggatgacggg
    10561 acgcgaaccg gtagcaatca cgaaactttt tgccgtaatt tgagtatgct cgtcgatttg
    10621 gacggtatgc tcgtcgataa atttagccga acccatgatg cgcttgccgg caggccactc
    10681 ttccacatcg gcaacgacaa agccgacaaa acggtcacgc tcggatttaa cgcgctgcat
    10741 gacctcttca ccgttgacga cgatgctgtc tttgtccaaa tgcacgccga acgggtcggt
    10801 atgcaatgcg tggtggcgcg cttctgcggc ggcaatcaag agtttggaag gcatacaacc
    10861 cacgcgcgcg caggttgtgc cgaacacatt gttttcaatc aggtaaacat tatccgaatg
    10921 taaacgggca ttgcgaaacg cacccatacc ggcagtaccg ccgccgatta cgacgacatc
    10981 cgcttgaatt tttttcataa tctttgtcct tccgttttcg tctgaaacat tgccgacaca
    11041 gaacatatcg gtttcagacg gcatttaaat attttcccaa tcaaaaacat cattcagata
    11101 aataaaaaca aaatttcgat tgagtgcaac aatatccaaa ttctcttata aatatagagt
    11161 tattcggatc ggcagtgtag aaccgcccga ataactctat ccggtatatc agacagttta
    11221 atcaaaccgc cttaagcaaa cagctatcag tttttagcca agtaagcttc taaatcttcg
    11281 ctgccgccga tgtatttacc gccgatgaag acttgagggg cagtcatctt gccggtaatg
    11341 gcgcgaacgg aagtaacggt tgcatctttg cccaatacga tttcttcgta agacaaacct
    11401 ttgtcttgca aagcttgttt ggctttagcg cagaattggc aacctggttt agtgaaaatt
    11461 gccacagact cttgagcttt ccaatcagga gcaacgaatt tcagcatagt atctgcatcg
    11521 gatactttga acggatcgcc cggttcttca ggttcgatga acattttttc aaccacgccg
    11581 tcgttaacca gcatggagta acgccaagag cgtttgccga agcccaagtc ttctttaccg
    11641 accagcatac ccataccttc ggtaaattcg ccgttgccgt caggaatcat gtggatgttg
    11701 tcggattctt cttcggcagc ccaagcgttc attacgaacg tatcgtttac agatacgcag
    11761 cagattgcgt caacgccgtt ttctttgaac gcgccgaaca attcattgta acgcggcagg
    11821 tgtgaagaag aacaagtcgg ggtaaatgca ccgggcaggg agaatacgac tacttttttg
    11881 cctttgaaca aatcatcggt agacacatct ttccaagtgt cgccgacgcg ggtgcggaat
    11941 actacggaag gtactttttg accggtacga tcttgcaaag ccattttaag ctccttaata
    12001 gattggtttg agaaataatt gtgaatcact gggcattcta ccccatgcaa aatgatttgt
    12061 ataatttata gttcaaataa tataaattat ttaaaactat caaacacaac tccgatttgc
    12121 ctgtttccca catataaagc cgaattagaa aaaaacaagc tgcaatgaaa agattttccg
    12181 gcaactgcgc ccttgcgccg caaatcagga caaaataccg tctgaaaggc tttcagacgg
    12241 tatttattca tacggtctcg gcttatttcg ccaattcggc acgcagtttt cgggttacgt
    12301 tcatcatcac ttggagttgt tccagagttt ctttccagcc gcgtgttttc aggccgcagt
    12361 caggattcac ccacagacgc tcaaccggta caacctcgat ggctttgcgc aacaggtgct
    12421 caacttcggc ttcagtcggt acgcgcgggc tgtggatgtc gtaaacaccc gggccgatgt
    12481 cgttcgggta tttgaattcg ccgaacgcgg tcaagagttc catatcggaa cgtgaagttt
    12541 cgatggtgat gacatcagca tccatagcgg cgatggcagg caggatgtcg ttgaactcag
    12601 agtagcacat atgagtgtgg atttgggtgc tgtcttcgca accggtagag gacaggcgga
    12661 aggattcgcc ggcccagttc aggtaggcat cccaatcggc acgtttcaaa ggcagacctt
    12721 cacggatggc aggttcgtca atttggatga ctttaatgcc ggctttttcc agatccaata
    12781 cttcgtcgtt cagggccagt gcgatttgtt tgcacacggt agagcgagga atgtcgttgc
    12841 ggacgaaaga ccattgcaga atggtgacag gaccggtcaa catgcctttc atcgggcgtt
    12901 tggtcaggct ttgagcgtaa gtagaccaag cgacagtcat ggcttcagga cggcttacgt
    12961 caccgaagat gatcggtggt ttaacacaac gtgagccgta gctttgtacc cagccgtatt
    13021 gggtgaatgc aaaaccgctc aacaattcgc cgaagtattc gaccatgtcg ttacgctcgg
    13081 cttcgccgtg taccagtacg tccaaatcca gtttttcttg ttcttcaacc accaaggcga
    13141 tttctttttt catcgcggct tcgtaatcgg cggcagacag ttcgcctttt ttgaaggctg
    13201 cgcgtgcctg gcggatttcg gtggtttgcg ggaaagaacc gatgttggtc gtcggcagca
    13261 gaggcaggtt caaccatgct tgttgcgctt tgatacggtc ggcaaatggg gatttgcgtt
    13321 ggtctgcgtt tgcaggcaaa tcggcaaggc gtttggcaac gtctgcacgg tggatttcgc
    13381 tgctgttggc gcgtgagccg gcggcagctt ggctggcggc cagttcttcg gcaacagaat
    13441 cacggccttc gttcaatgcg gctttcagaa cgcgcaattc ttgggttttt tgcagggtaa
    13501 atgccaacca agagtacagg tcgggtttgt tggctttcag tttttcttca actgacaagt
    13561 caaatggagt gtgcagcaga gagcaagagc tggagatcca caaacggtcg cccagtttgg
    13621 cttgcagagg ctcaacagtt tccaaaactt tgttcaggtt ggcgcgccaa atattgcggc
    13681 cgtcaatcac gccggcggac aggactttgt cgtagtcggc gaacgcgtcc agttgctcgg
    13741 gcgcgcgtac caagtcgatg tgcagaccgt caacaggcag ggatttcaac aatgcggcgt
    13801 gttcggcaac agaaccgaag taagtgctca acaggatttt ggcacttact ttgctcaaag
    13861 tagcgtaaac gtctttgtac gcttctaccc attctttagg caggtcaaca gccaaagcag
    13921 gctcgtcgat ttgaatccat tctgcaccgg cttcaaccaa agcgtttagg atttcaacgt
    13981 aaacaggcag cagtttaggc aacaggttca gacggtcaaa ttcgacagcg cctttttctt
    14041 taccaaccca caggaaggtc aacggaccaa caacggtcgg tttggctttc aggcccaaag
    14101 cttgggcttc ttgcagctgt tgaacgtagt gtttggcatt ggctttgaat tcagtatcgg
    14161 cgtggaattc aggcaccaag tagtggtagt tggtgtcgaa ccatttggtc atttcgatag
    14221 cgaattggtc tttgttgccc cgcgccagtt ggaagaattg ttccaaagac aggttttggc
    14281 tgtcgaagcc gaagcgggcg ggaatcgcgc cggtggcgac ttgcaggtcg aggatgtggt
    14341 cgtagaaagt gaaatcgcct acggcaacga aatcggcgtt ggcagcggcc tggtgtttcc
    14401 agtttttctc gcgcaagtct ttagcaacag ccagcaattc ttgctcgctg atttctttgc
    14461 gccagtattt ttcttgtgcg aatttcaatt cgcggaaggc gccgacacgc gggaagcctg
    14521 agaaatgtaa tgttgtcatg ttgaactctc cagttggatt ttgtaaggac cggttcaatc
    14581 cgaatcggcc ggtattgtaa atcagaatgg ctaggatggg gcggcgtttt gcttttcaaa
    14641 cggcctcgga tgccgtctga agccgttttc agctttaagg gcgcacgcct aaaatatggc
    14701 aaatggcgta agtcagctcg ctgcggttaa gcgtatagaa gtggaaatct ttcacgcctt
    14761 cgcgggacag gactttgacc atatcgatgg cgatacttgc cgccaccaga ttgcgcgtgc
    14821 cttggtcgtc gtccaaacct tcatacattt gcgacagcca gcttgggatt ttgacgttgg
    14881 ttacttgagc cattttaccg agctgcttga agttggtaac aggcaaaata ccggggacga
    14941 tttccacatc gatacccaac atcacgcagc ggtcgcggaa gcgcaggtag cgttctacgt
    15001 caaagaaaaa ttgggtgatg acgtggttcg cgcccgcatc gattttgcgt ttcaaattaa
    15061 tcaggtcggc ttgtgcggat ttcgcttcgg gatgcacttc gggatatgct gctacagaga
    15121 tgtcgaagtc ggcgacggag cgtaatagct taaccaagtc ttcggcataa aacggttttt
    15181 tctcgtaacc gggcggctcg tcgccgcgca gggcgacaat gcggcggatg ccgctgtccc
    15241 aatagtcttt ggcgatttgg cgcaattcgt cgggagaagc gtcgatgccg gtcaggtgcg
    15301 gcgcggcttc caagccggtt tcttgcttaa tgcgtttgac gatgccgtgc gtgcggtcgc
    15361 gctcgcctga gtttgcaccg taagttacgg aaacaaattt cgggtgcaag gtttgcaggc
    15421 gatggataga atcccacagc atggtttcca tttgttcgtt tttcggcggg aaaaattcaa
    15481 acgaaacgtt aatgtcgcct ttcaaatcgg aaaggctgtt gtttaacgca ttgatttctt
    15541 ttgcgtaatt catggctgcg taccttttat tatcttttat atgatatttt caggcaacat
    15601 aataaagttg cgccaaacat gagtcaattt caaaattttc agatttatca tgaaaatttc
    15661 taatatatta caaaaatagt taaatttcct tgtacaagcc ttctaattta tgataaagtt
    15721 tgttatgtta taacaattca actaaggcga tatatgaaac cgaatattca tcctgacaat
    15781 tatcgaaccg tccttttttt cgacagcagc gccaatgaag gctggttgat tcgttcttgc
    15841 gccgaaacac acggaaaaac gatggtttgg acagacggca aagaatatcc gctgttttcg
    15901 ctggatactt cttccgcatc ccatcccgtc tatactggca aacagcgcaa cgtcaacacc
    15961 gaaggccgtg ccagcaaatt caaccaacgt ttccaatccg tcatgtcatc gtttagaaag
    16021 gacaaataat gcaggttctg tcttcactca aaaccgccaa acaacgccac cgcgactgcc
    16081 aaatcgtcag gcgcaggggc aaagtttacg tcatctgcaa aagcaatccg cgttttaaag
    16141 cgcgccaacg ctgaccccct gcccttcctg cttcggttgt aaatcccctc ctaccgcaag
    16201 cccttttcaa aaaatgccgt ctgaaacaga aaaatgcgtt tcagacggca ttttttccgt
    16261 caatacgaat ccggaatgtg tttgaaatac agcataaaca gataccaaaa cagcgtcgcg
    16321 gcggccgcac cgagcagcac ccgggcactg atttcggcaa cttcgcgcag ggcgcgtttc
    16381 tgcctgcggc tgaagaggcg gttcataatc agcgcggcaa gtgcgaaaaa gaaaaaaata
    16441 cggaaaagtc tgcctatcat gtcgggaagc gaaaagtatg gttcgggcgc ggcggcatgg
    16501 ggaaggatgt ttcagcgcgt caggctgctt gtccgaccgt gtcaaaaagt catgccgttt
    16561 gcgatataat aacgcttttt gccgtatttg ttggaacagg atatgagtca aaatgcctcg
    16621 gtgggcattg taacgcccca aaaaattccg tttgaaatgc cgctggtttt ggaaaacggt
    16681 aaaactttgc cgcgtttcga tctgatgatt gaaacctacg gcgagctgaa tgccgaaaaa
    16741 aataatgcgg ttttaatctg tcatgcgctg tcaggcaacc atcatgttgc gggcaggcat
    16801 tcggcggagg ataaatatac gggctggtgg gacaatatgg taggacccgg caaaccgatt
    16861 gatacggaac gttttttcgt ggtcggtttg aacaatctgg gcggctgtga cggcagcagc
    16921 ggacctttgt cgatcaatcc tgaaacgggt agggaatacg gcgcggattt tccggtggtt
    16981 acggtgaagg actgggtaaa atcacaagcc gcgcttgccg actatctcgg catcgggcaa
    17041 tgggcggcgg ttgtcggcgg cagtttgggc ggtatgcagg ctttgcagtg ggcgatttcc
    17101 tatcccgaac gcgtgcgcca tgccttagtg attgcgtctg cgccgaaact gtccacgcaa
    17161 aatatcgcgt tcaacgatgt ggcgcgtcag gcgattttga ccgaccccga tttcaacgaa
    17221 ggacattacc gcagccataa taccgttcct gcacggggct tgcggattgc ccgcatgatg
    17281 gggcacatta cctatcttgc cgaagacggt ttgggcaaaa aattcggacg cgatttgcgt
    17341 tccaacggct atcaatacgg ttatggcgtt gaatttgaag tagaatccta tctgcgctat
    17401 cagggcgaca aattcgtcgg gcggtttgat gccaacacct atttgctgat gaccaaagct
    17461 ttggactatt tcgatccggc ggcggatttc ggcaacagcc tgacccgcgc cgtgcaggat
    17521 gtgcaggcaa aattctttgt cgccagcttc agcaccgatt ggcgtttcgc gcccgaacgt
    17581 tcgcacgaac tggtcaaggc cctgattgcc gcccaaaaat ccgtgcagta tatcgaagtc
    17641 aaatccgcac acgggcacga tgccttttta atggaagacg aagcctatat gcgtgcggtc
    17701 gccgcctata tgaacaacgt ttataaggaa tgtcagcaat gaacctgcgc gatgatttgc
    17761 aactgattta cgaccggata cccgaaggca gccgcgtctt ggacttgggc tgcggcgacg
    17821 gcgaactgct tgccgcattg gtcgaacaca aaaaatgcag cggctacggc atcgaaatcg
    17881 acaccgacag cgtgattgcc gcgatgtcgc gcggcgtgaa tgttatccaa gccgatttgg
    17941 aagaaggttt aaccgcattc aacgatcaaa gttttgatgt gattgtgttg agccaaacca
    18001 tccaagcgat gcagaatacc gaaaaaatcc tgcgctgcct gatgcgcgtt gccaaacagg
    18061 cgattgtcag cttcccgaat ttcggctact ggcgcaaccg cgtccaaatc gcactcggcg
    18121 gacatatgcc ggtttccgaa cgtatgccct accattggta cgacacgccc aatatccatt
    18181 ggtgcacgct caaagacttt gatttgttgt gcgccaaaaa caacatccgc gtcctcgaac
    18241 gcgccgtgat gacgggcaac agacaggtca aacatttccc caaccttttg ggcagccttg
    18301 cattttaccg cgtcgggtaa gacagattga gaaccttgtc ggcattaaca ggttcttcgg
    18361 ttctattaac aacaggccgt ctgaaaaatg aaacggtagg ttttcagacg gcctctttaa
    18421 tactggaaac aatagaatga aaaacaaaac ctcatcactt ctcttatggc ttgccgcaat
    18481 gatgctgacc gcgtgttccc cgagcaaaga agataaaacg aaagaaaacg gcgcatccgc
    18541 cgcctcgtcc acggcatccg ccgcttcgtc ttccgcgccc caaaccgatt tgcaaccggc
    18601 cgcatccgcc cctgataacg tcaagcaggc agaaagcgtg ccgccgtcaa attgcaccga
    18661 cctgcacccc gccaccggca ttgacgatct catgcagcaa atcgccgaac acattgactc
    18721 ggactgtctg tttgcccttt cccatcacga actggaaacc cgtttcggct tacccggcgg
    18781 cggctatgac aacatacagc ggctgctgtt tcccgacatc cgccctgaag atcccgacta
    18841 ccatcagaaa atcatactgg caattgaaga cttgcgttac ggaaagcgca cgatcagccg
    18901 gcaggcacaa gatgccttga tggaacagga acgccgcctc cgagaagcga cgctgttgct
    18961 gatacagggc agtcaagaaa cccgcggaca aggcgaggag ccgaaacgca cgcgttattt
    19021 tgaagtttcg gcaacccctg cctattcgag ccggcacaac aacggacttg gcggcaattt
    19081 ccaatacatc ggccaattgc ccggctatct gaaaatacac ggagaaatgc ttgaaaacca
    19141 atcactcttc cggctgtcca accgtgaacg caatcccgac aaaccgtttt tagacatcca
    19201 ttttgacgaa aatggcaaaa tcacgcgtat tgtcgtttac gaaaaaaaca tctacttcaa
    19261 tccaaacttg gggcgaagat aaaaaaatgc cgtctgaaac gttttcagac ggcatttttc
    19321 aatgcaacaa atcaagcgcg tttgcggaat tcgccagtgc gggtgtcgat ttcgattttg
    19381 tcgccgtttt cgatgtaaga catcacttgg atttcggtgc ccccgaccag gcgcgcggtt
    19441 ttcatcactt tgccggaagt atcgcctttg acggcaggct cggtatactc gacttcgcgt
    19501 acgatgatgg tgggcagttc tacggagatg gggttgcctt cgtagaaggt aacttcgcat
    19561 tggtcttcca tgccgtcaac gatgaatttc aacgcgtcgc cgatgttgtc ggcttcgatt
    19621 tcgtattggt tgaattcttc gtccataaag acgtacatcg gatcggcaaa gtagctgtac
    19681 gtacagtttt tgcgggacag gatgaccacg tcgaatttgt cgtcggcttt gtaaatggtt
    19741 tcggaagccg cgccggtcag caggtttttc agtttcatgc tgactttggc ggaagagcgg
    19801 ccgcctttga tgtattcggt tttttgaacg accataggat cgttgccgac cataaataca
    19861 ttgccggcgc gcagttcttg tgctgttttc attgtgattt tccaatcaaa tcggttgtcg
    19921 taaaacgcgc tattctagcg tattttttga tgctttgaaa caaaggcggc gagtttttcg
    19981 gatgcggaag gctgcccgaa aagataacgg ctccaatcct ccgcgccttg ccgccagccg
    20041 ttttgatgtt gttgcagggt ttgccaacat tcgaggcgtt gcgtggcgga taaagcctct
    20101 ccgccgttga ggtcgtctga aaggcggcgg tgtaccgatg cggtttcggg cgagtaaaat
    20161 ccatgcgcct tatcccaaaa ggcgtggagt ttatcgagat ggacattctc gtcttgcggg
    20221 tagatgtgcc aaaagaaggg tttgcccgca agctgggcgc gcacgaaact gtcttcgcct
    20281 cgtatcacag cgcagtcggc gaggtgcagc agtttgtcga agtcctgttg cggaacgaaa
    20341 gggattttga caaggcggac cgaggtcgtc tgaaaaacat cgccgtcgtt ttgcagggca
    20401 tcttgcggaa taacgccgct ttgtttgagg ctgtcgatga tttgcgcccc cgccagcagc
    20461 agggtcaacg cgctgcctgc ctgttgccac atgtccagcc acttcgccca aacatcgctc
    20521 cgatagccga aaagcagcca ttcgggcgcg tttttttcgg gaagcatcag cctcttgcgc
    20581 aaggctccgc tatcgaaacg gacggcttcg cagtaatcgc gttcgcgtat cagtccgccg
    20641 cttttttcgc tgaagcccat aaaccaaaaa tatttttgaa caccttcctg cggcgaaggc
    20701 atcaggtgca gcctttcatt gctttcctcc gcgctcaaat attcccaatt cagccaaagc
    20761 ggcttgtgtc tgcggataat gttcaacaca ttttcgggca ggtcgcaggc aaaagtttcg
    20821 atgacggcat cgggaacagg cgcggtatca atatctgccg catcggaatg ccaagtgcgg
    20881 acatgaatat cctgatgaac gcagggaaca tcgggcaaat cagggcaaag cgcacgcaag
    20941 gcggacacat cgtccgtcca caaatgcacc tgccaaccga gttcgcggtg caaaacacgg
    21001 gcgaaccgcc acgaaacgcc gatgtcgccg aaattgtcga tgaccttgca aaaaatccaa
    21061 cagacaaaag gaggagtatt catagaaata acggaaacgg gctaaaacgc gccgaaatag
    21121 tggaacaaat acacaatcag caaaacaatc ggaataaaca gaaacatagt ttgacgtttc
    21181 aaatcaaact cttgcttcaa atgatgcttg atggtgtcga aaaggacgaa tttgaacaaa
    21241 aacgtcgctt cgcaggcaag gatgatgccg ttaatcaaat gctggggttt gtcttcgggc
    21301 ggattggcgg cgaaaacggc aagcgttgcc aacgggatga ggcagacggc aagccagcgg
    21361 acaagcaggt ttcgagacat tgcaaggctt tcaaaaaaag caaaacgcca ttatcgcccc
    21421 aagcgcacgc cttgtctaac ggacgcaccg gagatgccgt ctgaagcctt gccgccggaa
    21481 aacgccgcac cggagatgcc gtctgaagcc ttgccgccgg aaaacgccgc accgccgaac
    21541 ctttaaatac gcccgggacg ggcgttccct gaaaaatcgg acgctgccgc cgatgcctgc
    21601 ggacaaggcg gcatcgggcg cgggcatatt gaaaacccgg tgcgtcggtt tgaacgcatt
    21661 ttaaaaggca tacagcaagc ccgtcccttg ccgtcggcgt gcttccgcaa agcaaaatat
    21721 aagggcggcg cgctttccgc tataatccgc attttcgcaa ccctgaaaca tcatgccgat
    21781 cccgaaagcc tttgccctgc tcgggccgac cgcctgcggc aaaaccgcgc tcgccctcaa
    21841 aattgccgaa accctgcctg tcgaaatcat cagcctcgat tccgcgctgg tttaccgcga
    21901 tatggacatc ggcacggcga aaccgtctca tgccgagtgt tcctttgtac cgcaccacct
    21961 tatcgacatc attacgcctg ttcagacata cagtgccgcc cgttttgtcg aagactgcac
    22021 gcgtctgatt ggcgaaatca cggcgcgtgg gaaatatccg ctgattgtcg gcggcacgat
    22081 gatgtatttc cacgccctta cccaaggttt gaacgatttg cccgaggccg atgcctgcct
    22141 gcgtgccgac ttggacgaac aaaaacagat gtacggcttg gatttcctct accgcaccct
    22201 gcaaaaagtc gaccctgaaa cagcatgccg tctgaaaccg aacgacagcc agcgtatcgg
    22261 acgcgctttg gaagtttatt atttaaccgg caaaccgatg agcacgcatc tcggcagccc
    22321 aacctcccat acgctccctt ttgatttgca taccgccgcc ctgattcccg aagaccgcgc
    22381 ccgactgcat gaaaacatcg ccctgcgttt ccacctgatg cttgaacagg gctttatcgg
    22441 cgaagtagaa aacctgcgcc gcctctatcc cggcctgacc gccgactccc ctgccatacg
    22501 ctgcgtcggc taccgtcagg catgggaata tctggacgga aaaaccgatt tcccggcatt
    22561 tgtcgagaaa ggcattgccg ccacgcgcca acttgccaaa cgccaactga cttggttgcg
    22621 caaaacacct ttagactgcg ttgccgaccc gttttcagac ggcacttcat gcacacgcct
    22681 gattgaggcg gcaaaacggt ttttcggcgc ataaaccatg ctgaccacac cgccgctcgc
    22741 ccccaaaacc gtcgccgccc tgcacaggct cggtatccgt acgctcgaag aactgcggca
    22801 aaacggttcg gtcaaagcct ttctgctgct caaagcatcg ggtttgaccc tcacgaaaag
    22861 cacgctgtgg cagcttgagt cgctgctgaa cggcacactc ccgcaagaaa tcacgcaaca
    22921 cgacaaaacg cgcctttttg ccgaattgaa aaaccatccg cccgtcgccg cctttccgcc
    22981 gcaggaagaa atggaacact tcatgcgcga agcactgcgc caagcagaac aatccgccgc
    23041 cgacggcgaa atacccgtcg gggcagtcat cgtttcagac ggcaaaatca tcgcatcggc
    23101 acacaacacc tgcattgccg actgcaatgt cagccggcac gccgaaatca acgccttggc
    23161 acaggcaggc agcgaaatgc aaaactaccg ccttgacgga tgcgacatat atatcaccct
    23221 cgaaccctgc gccatgtgcg cgtccgcact gatacaggca cgaatcaggc gcgtgatcta
    23281 cggggcggca gaacccaaaa ccggcgcggc aggcagcatc gtcaacctgt ttgccgacaa
    23341 acgcctcaat acgcacaccg ccatacgggg cggaatcctg caagaagaat gccgcgccgt
    23401 gttaagccgt ttttttcaaa acaaaagaaa aggttgaacc tatgaccgta ctgaccgtca
    23461 tcctcgccct cgccctgata gccgtcggca cggcgggcat cgtttacccc gccctgcccg
    23521 gattggcatt gatgtttgcc ggaacatggc tgcttgccta tgccggcggc taccaaatct
    23581 acggcgcggg cgttttgtgg acggtcggac tcatcagcct tgccggcata ctggcggact
    23641 atgtggcagg catatggggg acaaaatata ccggagcggg caagctcgcc gttcgcggcg
    23701 cattggtcgg cagcatcatc ggcatatttt tctcccttcc cggactaata ctcggtccct
    23761 ttatcggcgc ggcggcaggc gaactgatcg atcggcgcaa tatgcttcag gcaggtaaag
    23821 cgggcttggg tacgctgttg gggcttatcg tcggtacgac attcaaaatc ggctgcgccg
    23881 tatccatctt gtttatcctg ttggtgaaat acatcgccta cctgttttaa ccccttcaga
    23941 cggcataccg gcaaattgct ataatgccgt ctgaaccctt tttacaaagc accatatggc
    24001 aaaccaaaga cctatctacg gcttccatgc cgtcaacgcc cgattgtggc aaaaccccaa
    24061 atccatcgtc gaactctatg tccaagaagg caaaaacgat gtacgcacgc gcgaagtgtt
    24121 ggaaaaggcg gcaaacgaaa acatccgcgt atattttgcc gatgccgacc gcctcaacgc
    24181 catcagcaaa ggtgcgcgcc atcagggcgt ggtcggattt atcgatgcct ccaaaaacca
    24241 cgtccacctc gaagacgtat tggaaaactt aagcgaaccg ccgctgctgc tgatactcga
    24301 cggcatcacc gacccgcaca acctcggcgc atgcctgcgt accgccgacg caatgggcgt
    24361 acatgccgtc atcgcaccga aagacaaaag cgcggggctg aacgccaccg tcagcaaagt
    24421 cgcctgcggc gcggcggaaa ctgtccccta catcactgta accaacctcg cccgcaccct
    24481 gcgcgagctg aaagaatacg gcatctggat tatcggcacc gacatgggcg gcgacgccga
    24541 cctttaccat tgcaatctgc ccgacagcgc ggcgtgggta atgggcaacg aaggcgacgg
    24601 tatgcgccgc ctcacgcgtg aacactgcga catgctggtg tccataccca tgttcggcac
    24661 ggtcgaaagc atgaacgtct ccgtgtccgc aggaatggta ttaagtgaaa cccgccggca
    24721 acgcgtattg aaaaacgaaa aagcgtaagc ctcaaaaaca atgccgtctg aaacagggat
    24781 tctgtttcag acggcattca ttttcaaaat cagtgttcca agtggtcggt atcaaacttg
    24841 accgccgcct cggttttatc ttcgggcaag accaaattca acaagactgc cgtaatgccg
    24901 ccggcggaaa tagagttttg gaacaagacg ggcagattct taaacacttc cggttcaaac
    24961 gcgacaccca agcccaaacc gaccgacgtt gccgcaatta ccgcctcgcg cctgcggatg
    25021 ccgtggctga ccaaaatccg cacgcccgca atcgcaatca agccgaacat caaaaccatc
    25081 gcgccgccca acaccggact cggaatcgtc gtaaacgcgc gtccgacaac ggggaacaga
    25141 cccaacagca ccaaaatcac ggcaatatat ttgcccacat ggcgcgaagc cacgccggtc
    25201 atctgaatca cgccgttgtt ttgcgcaaac gtcgtcagcg gcagcgaacc caaagccgtc
    25261 gcaatcaccg acaccaagcc gtcagccaac acgccgccgc gcaggcgttt ggtgtattcc
    25321 tcgccttcaa tcggctggtc ggacaccatt gccgtcgcgg ttaaatcgcc gaccgcctca
    25381 aacacgctca acaagaaaat cgcgcccgcc acaataaacg cgtgccagtc aaaagcaaaa
    25441 ccatatttaa acggtacggg cagcgtaacc agcggcaggt tttgcagcgc ggaaaaatcc
    25501 accttgccca aaaacagcgc gacaatgtaa ccggcaatca gaccgaccgc gatgccgctc
    25561 atccgcaaca gcgggttttt catgcagttg aatatcaaca caatcagcaa caccagcgaa
    25621 gccagcccca agttttccat cgagccgaac gtgccgtccg ccttcgcgcc gaagccgccg
    25681 ccgaaatcgg taataccgac gtgcaccaaa ctcaagccaa tcagcatcac gaccacgcca
    25741 ctgaccgtcg gcgtaatcac ttttttcaaa tacggcagca gccacgccga gaaacacacc
    25801 aaaaacgcgc cgacaaacga tacgcccaag agcgtcgaaa tcatcgcatc cttagtcaaa
    25861 ccgccctctt tcatccccgc gccgagcgca atcatgacgg taacgaacga gaaattcacc
    25921 gactggatgg acagcatccc cgaaccgacc ggcccgaagc ggttgacctg caaataagtg
    25981 ccgacacccg acgcaaccat cgccatcgac acgagatacg ccgtcatctc caccggcaat
    26041 tccagcgcgc cgcccacaat cagcgcgggc gtaatcatcg gcacaaaaat cgccaaaaga
    26101 tgggtaaccg cgctcaagag cgcattaccg aacggcggcc tatcctccaa accgtacacc
    26161 aaatcaggcg catccgcccc ttttcccata tgaccgctca taatgtttcc ttgcttcaaa
    26221 tgttgaaaaa accaaagcgc ggattgtagc gaatccgcca taccgcttca atttcgattt
    26281 cggacggcat acgcgcccgt ccggcaaaca gattccgaac cgtcgccgcc ggaatatcgt
    26341 ttacaaaagg caataacctg atttcaaaca caaaaataca aacccgacgc atccgccgtc
    26401 aagccgcccc gtgcataccg gacccgccgg atttcacacc tgcggtaaag taggctacaa
    26461 tcagaagcat taccgccccc tacaccacaa ctgataaaag gttctgtatg ttacaaggta
    26521 gcctggttgc cctgattacc ccgatgaatc aagacggcag catccattac gaacaactcc
    26581 gcgatttgat cgactggcac attgaaaacg gcacggacgg catcgttgcc gtcggcacga
    26641 caggcgaatc cgccaccctc tccgtcgaag aacacaccgc cgtcatcgaa gccgtcgtca
    26701 aacacgttgc caaacgcgtc cccgtcatcg ccggcacagg ggcaaacaac accgtcgaag
    26761 ccatcgccct ttcccaagcc gccgaaaaag ccggggcgga ctacaccctc tccgtcgtcc
    26821 cctattacaa caaaccttcg caagaaggca tgtaccgcca tttcaaagcc gttgccgaag
    26881 ccgccgcgat tccgatgata ctctacaacg tacccggcag aaccgtcgtc agcatgaaca
    26941 acgaaaccat cctgcgcctt gccgaaatcc ccaacatcgt cggcgtgaaa gaagccagcg
    27001 gcaacatcgg cagcaatatc gaactcatca accgcgcgcc cgaaagcttc gtcgtccttt
    27061 ccggcgacga ccacaccgcc ctgccgttca tgctctgcgg cggacacggc gtgattaccg
    27121 tagccgccaa cgccgcgccc aagctctttg ccgatatgtg ccgcgccgcg cttcaaggcg
    27181 atatcgccct tgcacgcgaa ctcaacaacc ggctgattcc gatttacgac accatgttct
    27241 gcgaacccag ccccgccgcg ccgaaatggg cggtatccgc cttgggcaga tgcgaaccgc
    27301 acgtccgcct gccgctcgtt ccgctgacgg aaggcggaca ggcaaaagtc cgcgccgccc
    27361 tgaaagcctc aggacaactt taaatatagc catgccgtcc gaaccgttcg gacggcataa
    27421 cgcaacaaac actttaatat ccatcacaca ggatgacacg atgacccata tcaaacccgt
    27481 cattgccgcg ctcgcactca tcgggcttgc cgcctgctcc ggcagcaaaa ccgaacagcc
    27541 caagctcgac taccaaagcc ggtcgcaccg cctgatcaaa ctcgaagtcc cacctgattt
    27601 gaacaacccc gaccaaggca acctctaccg cctgcctgcc ggttcgggcg ccgtccgcgc
    27661 cagcgatttg gaaaaacgcc gcacacccgc cgtccaacag cctgccgatg ccgaagtatt
    27721 gaaaagcgtc aaaggtgtcc gcctcgagcg cgacggcagc caacgctggc tcgttgtcga
    27781 cggcaagtct catgccgaaa tctggccgct cctgaaagcc ttttggcagg aaaacggctt
    27841 cgacatcaaa tccgaagaac ccgccatcgg acaaatggaa accgagtggg cggaaaaccg
    27901 tgccaaaatc ccccaagaca gcttgcgccg cctattcgac acagtcggtt tgggcggcat
    27961 ctactccacc ggcgagcgcg acaaattcat cgtccgtatc gaacagggca aaaacggcgt
    28021 ttccgacatc ttcttcgccc acaaagccat gaaagaagtg tacggcggca aagacaaaga
    28081 cacgaccgta tggcagccct ccccgtccga tcccaacctc gaagccgctt tcctgacgcg
    28141 ctttatgcaa tatttgggcg ttgacggaca gcaggcggaa aacgcatcgg caaaaaaacc
    28201 tacctttccc gccgccaacg aaatggcgcg tatcgaaggc aaaagcctga ttgtctttgg
    28261 cgactacggc agaaactggc ggcgcaccgc gctcgccctc gaccgcatcg ggctgaccgt
    28321 cgtcggtcaa aacaccgaac gccacgcttt cctggttcaa aaagccccga acgaaagcaa
    28381 tgcagttacc gaacaaaaac ccggcctgtt caaacgcctg ctgggcaaag gcaaagcgga
    28441 gaaacctgcc gaacagccgg aactgattgt ctatgccgag cctgtcgcca acggctcgcg
    28501 catcgtcctg ctcaacaaag acggcagcgc atatgccggc aaagacgcat ccgcattatt
    28561 gggcaaactc cattccgaac tgcgttaaac cattcaaaac aaaagcagct tccaaacaga
    28621 agctgctttt ttatgcggaa ctttttacaa caaatggggc aaaatatcct caactgcctg
    28681 aaccaacgca tccaccaaag gcggatcgaa cgcacgatgc cctgcctgaa ccaccctcaa
    28741 ttccgcttcg ggaaaggctt tcgacagcgc ccacgcactc tgcatcggcg tacacaaatc
    28801 ataccgcccc tgtacgataa tagtcgggat atgccgtatt ttgccgatat tgttcaaaat
    28861 cgccctatcg ccctgcaacc aaccgccgtt gacaaaataa tggttttcca aacgcgcgat
    28921 tgccagcgag gcataagcat cttcatccac ttcctccggc tcgaaacgga tcagatagct
    28981 ttcccaatcc gcccaagcct tcgcggcaga caggcacact tcttcatctt gatgaaacag
    29041 caatccgtga tacgcctcaa tcagccggtt ccgccgattt tcagcaatcg gcgcgacaaa
    29101 tttttgccat tgttccggat aaatccggct cacaccgccc gcctcgttca gccacaccgt
    29161 ttcagacggt ctgcacaaaa atatcccgcg caacaccaat ccctttaccc gttcaggatg
    29221 ggtttgggca taagccagcg acaaagtgct gccccacgaa ccgccgaaca ccagccattt
    29281 cccgataccc agcatttcac ggactttttc aatatccgcc accaaatccc aagtcgtatt
    29341 gtcttccgca caagcatacg ggcgcgaacg tccgcaaccg cgctggtcga tgatgacgat
    29401 gcggaacaca tcgggattga aaaaaccccg acattcaggc gaagcccccg cgcccggccc
    29461 gccgtgtaaa aaaataaccg gcacaccgtc gggattgccc gattcctccc aataaatttg
    29521 atgaatttca gacacctgca agtatccgct atgaaaaggc tgttttatct catacatcat
    29581 cttctcccat attgcttctg acaatccgaa cattcccaca cgcaacatca cgcacgagac
    29641 acaagccgcc ccgaaaggca aatgccgaaa aatcgcgata tagtgaatta acaaaaatca
    29701 ggacaaggcg acgaagcctc agacagtaca gatagtacgg aaccgattca ctcgtgcttc
    29761 agcaccttag agaatc
//
LOCUS       DAWKQJ010000006        28960 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000006 DAWKQJ010000000
VERSION     DAWKQJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.006"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(287..4456)
                     /locus_tag="WJL20_000202"
     CDS             complement(287..4456)
                     /locus_tag="WJL20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ7682413.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMHRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASATNWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4511..6358)
                     /locus_tag="WJL20_000203"
     CDS             complement(4511..6358)
                     /locus_tag="WJL20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ7682414.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAS
                     DNVKTMLRSKGYFSSKISLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGSKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNALHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6888..8117
                     /gene="sstT"
                     /locus_tag="WJL20_000204"
     CDS             6888..8117
                     /gene="sstT"
                     /locus_tag="WJL20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ7682415.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8229..9509
                     /locus_tag="WJL20_000205"
     CDS             8229..9509
                     /locus_tag="WJL20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7682416.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGNWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9585..9800)
                     /locus_tag="WJL20_000206"
     CDS             complement(9585..9800)
                     /locus_tag="WJL20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682417.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9793..10008)
                     /locus_tag="WJL20_000207"
     CDS             complement(9793..10008)
                     /locus_tag="WJL20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ7682418.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10083..11426)
                     /gene="argG"
                     /locus_tag="WJL20_000208"
     CDS             complement(10083..11426)
                     /gene="argG"
                     /locus_tag="WJL20_000208"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ7682419.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            11753..11893
                     /locus_tag="WJL20_000209"
     CDS             11753..11893
                     /locus_tag="WJL20_000209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682420.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11890..12690
                     /locus_tag="WJL20_000210"
     CDS             11890..12690
                     /locus_tag="WJL20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ7682421.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12730..13716
                     /locus_tag="WJL20_000211"
     CDS             12730..13716
                     /locus_tag="WJL20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ7682422.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14373..14635
                     /locus_tag="WJL20_000212"
                     /pseudo
     CDS             14373..14635
                     /locus_tag="WJL20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14673..15047)
                     /locus_tag="WJL20_000213"
     CDS             complement(14673..15047)
                     /locus_tag="WJL20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682423.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(15220..15576)
                     /locus_tag="WJL20_000214"
     CDS             complement(15220..15576)
                     /locus_tag="WJL20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ7682424.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15719..16062)
                     /locus_tag="WJL20_000215"
                     /pseudo
     CDS             complement(15719..16062)
                     /locus_tag="WJL20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16221..16621)
                     /locus_tag="WJL20_000216"
                     /pseudo
     CDS             complement(16221..16621)
                     /locus_tag="WJL20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16625..17365)
                     /locus_tag="WJL20_000217"
                     /pseudo
     CDS             complement(16625..17365)
                     /locus_tag="WJL20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17435..17752)
                     /locus_tag="WJL20_000218"
     CDS             complement(17435..17752)
                     /locus_tag="WJL20_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682425.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17749..18360)
                     /locus_tag="WJL20_000219"
                     /pseudo
     CDS             complement(17749..18360)
                     /locus_tag="WJL20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18444..18746)
                     /locus_tag="WJL20_000220"
     CDS             complement(18444..18746)
                     /locus_tag="WJL20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682426.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(18925..19131)
                     /locus_tag="WJL20_000221"
     CDS             complement(18925..19131)
                     /locus_tag="WJL20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682427.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19097..19846)
                     /gene="mafB"
                     /locus_tag="WJL20_000222"
                     /pseudo
     CDS             complement(19097..19846)
                     /gene="mafB"
                     /locus_tag="WJL20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20272..20616)
                     /locus_tag="WJL20_000223"
     CDS             complement(20272..20616)
                     /locus_tag="WJL20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682428.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20654..20977)
                     /locus_tag="WJL20_000224"
     CDS             complement(20654..20977)
                     /locus_tag="WJL20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682429.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(20980..21168)
                     /locus_tag="WJL20_000225"
     CDS             complement(20980..21168)
                     /locus_tag="WJL20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ7682430.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21238..21573)
                     /locus_tag="WJL20_000226"
                     /pseudo
     CDS             complement(21238..21573)
                     /locus_tag="WJL20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(21679..22050)
                     /locus_tag="WJL20_000227"
     CDS             complement(21679..22050)
                     /locus_tag="WJL20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7682431.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(22047..22298)
                     /locus_tag="WJL20_000228"
     CDS             complement(22047..22298)
                     /locus_tag="WJL20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682432.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(22430..22636)
                     /locus_tag="WJL20_000229"
     CDS             complement(22430..22636)
                     /locus_tag="WJL20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682433.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(22646..23491)
                     /gene="mafB"
                     /locus_tag="WJL20_000230"
                     /pseudo
     CDS             complement(22646..23491)
                     /gene="mafB"
                     /locus_tag="WJL20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(23588..23800)
                     /locus_tag="WJL20_000231"
     CDS             complement(23588..23800)
                     /locus_tag="WJL20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682434.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(23876..24235)
                     /locus_tag="WJL20_000232"
     CDS             complement(23876..24235)
                     /locus_tag="WJL20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7682435.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(24240..24956)
                     /locus_tag="WJL20_000233"
                     /pseudo
     CDS             complement(24240..24956)
                     /locus_tag="WJL20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(25173..25556)
                     /locus_tag="WJL20_000234"
     CDS             complement(25173..25556)
                     /locus_tag="WJL20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682436.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(25522..26967)
                     /gene="mafB"
                     /locus_tag="WJL20_000235"
     CDS             complement(25522..26967)
                     /gene="mafB"
                     /locus_tag="WJL20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ7682437.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(27007..27969)
                     /gene="mafA"
                     /locus_tag="WJL20_000236"
     CDS             complement(27007..27969)
                     /gene="mafA"
                     /locus_tag="WJL20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7682438.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAVYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(28153..28872)
                     /locus_tag="WJL20_000237"
     CDS             complement(28153..28872)
                     /locus_tag="WJL20_000237"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ7682439.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
BASE COUNT         7043 a         6625 c         6873 g         8419 t
ORIGIN      
        1 acctgtgtat cgctgtaaat cttagagatg gcggaatata gcggattaac aaaaaccggt
       61 acggcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctca agcaccgagt
      121 gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttatcct gatttttgtt
      181 aattcactat atctattctg ttcccttgca ggcggacggc ggcggcggac ggtggaaaag
      241 caggttttca aatgtccggt ttggcggcgc gccgtctgaa aaccgcttat tttcctttgc
      301 tgtttccggc ggagtctttt ttgtccgaac cggagaagcg gtcgaaacgt atggtgtatg
      361 tcagctcgcc gcccgacgaa cggctgccga tacgggcaac cgcctgtatg gcgcgggtca
      421 gccggtaaat cagtttgacg gactgttccg cgctggagat gctgtattcg tagccgatgt
      481 agagtttgcc ggtcagttgt ttgccgacgg tcagcacctg ttcggcgggg ttgagttcgc
      541 cggtttgcgc gttgcggctg cgcttgctgg taaagcccaa atcatccacc agcccgatgc
      601 ggtcgttgat ttgcccggca agcagcgcgc ctgcggctgc ggacagggcg gcattgtcgc
      661 cgctgctgcc gctgccggcg cggttgagga tgagccaaga gagcttgtct ttttcactca
      721 tcggttcgtt tgccgtcagc gtaatgcgcg ggctgttgag gctgcccaat atttccacgc
      781 ccgcaccgac gggggaaagg cggcgttcgg cgcggatgtt gaggttgggg tcgttgagcg
      841 ggccgacaaa ggagaccgtg cctttggtaa tgtccaaatc ctgcccgtat gccttataac
      901 gccctttgat gacgcggacc gtgcccacgc cccgcacgct tccgcccgat tgggcggtca
      961 gggtcagttt gccgcctatg gtaacgtccg cgccgtagcc ggcgaagcgg atgccgtcat
     1021 tgaggtctaa agtcaggttc atattgacgg ggagcggtgc cgccgcctct tttttgactt
     1081 cgcctaatac gacgacatcg tcgccgacgg acggcatcga ggatttttgc gaaccgaaca
     1141 gcccctgatc cgttttaatc atcccggtaa cggatatgcc tttttgcggc gaatagcgca
     1201 ggcgggtgtt gccggaaacc gtcaggcggc ggttggggcg ggacaggatg cggtatttgt
     1261 cgaacaccgc gccgatatcg acatcgggtc cgctgttttc cataccgacc gtaccggaga
     1321 gttccgccgt cccttcgtga cggaatttca ggctgtcgat tacccatttc ctgcctgcga
     1381 tatgcgaacg cagcgaaccg ttgtccaaga tgatgccttg ggtttggttg cggtaataga
     1441 gcttgtcgcc gttgatgctg ccgcccaagt gcgggtcggc gatgctgccg ccgagggtta
     1501 cggcggcatt caggctgcct ttgacggttt gtccgacggg caggaagttg cggaatactt
     1561 cggcatcggc aacggtcagg ttgagtttcc cgcccagggg cgaggtactg aaagaattac
     1621 tttgcccgac ggtgatgttg ccgttgattt tcccgtagtt gctgctgccg ttgacggcgg
     1681 cattgacgga cggagagccg acccgtccgc cgatttgcgc ggcggcattc aggctgccgg
     1741 taatgttttg cgcggcggcg ggcagaaagg gcttcaatgc gcccaagtcg ggaagggagg
     1801 cggtaatcct gccgccgagc ggtgcatttg ccatattgcc gccgaaggcg ttgccgatgt
     1861 ccaaatcggc gttaatccgc ccgaaacgcg cgccgccgtc aagcaggatt ccgatgcggt
     1921 cgttttgaaa gcgcgttttc agggaaaatg cgttcaaacc caaagcctgc ccgccgggca
     1981 atacggcatc gccgctttgc cggctgatat tgaggtagcc gcgcgcgttg cgcccgtagg
     2041 cgacatccca gtcgccgttt aaaaccagat tgtgttcgaa gggcggtttg aagaaattgt
     2101 gcaactcggc gatatgcaga ccgtgtgcgc cgccttttgc cgatatgccg gtttttttat
     2161 cccaagaaaa gtgttgcagg ttgaggctgc cgcccattgc ctgccaattt gtcgcacttg
     2221 ccgccacgcg ttccgcaccg gcttcgagcg tcatacggtt ttgcagcttg aggttgaatg
     2281 cgccgccgat gtcgaggatg ccgatgctgc ctttccatcg ggtaagttcc ctgttgatgc
     2341 cgcctgaagc gtccaaatcg aatttgaacg gtttgccatc cagcgtcatg gcggcgtgtg
     2401 tgcggatgcg gtgctgcacg cccgtgccgt ccagcatcag gtcggcggta tcgacaaccg
     2461 ccgctccgcc cgacagcgaa aggcggctgc ctttgatgtc ggcgcgtatc gggcggcttg
     2521 tgtcgggcga acctttgagc gtgaaatcga gcgaacggat gtctgccgcc ttgccgatgt
     2581 gcaggttgcg cgccgcgccg gaaaggtcgg tttcaaaggt tcggatgccg ccgtccaaat
     2641 cgccggaaag gtgtccgcgt acatttaaag accccgcgag tccgaaaccg aaacgggata
     2701 aatcgggtgc ggtgatattg aggttaagcc ggtcgccttt tttgccgaag ccgccgtctg
     2761 ttttgatgat gttccgcccc agccgcaaat cgacggcggc acgcggaagg tggcgggact
     2821 cgtaaacaat gtcggcactg ccggcaatcg gtacgccgtt gaacgtgccg ggtaaaaacc
     2881 gcattttgcc tgtgaatttc tcttttgcca gttcgccggc aaggtttatt gagccgttga
     2941 tattgcctgc cggaagttgc ggatcgatgc gcgaagggtc gaatgcgcgg gaacggatgt
     3001 ccagcttgag caggcggtct ttaaacagct cgagatagcc ttgcgcggtc aggctgcctt
     3061 gcccggcggc gatgttgacg gtgtcgagca ccagtttccg ctgtccgttt gcggggtcgc
     3121 ttgcaatggc gaggctgccg tccgtgcgcg ccgtgccgat gccgagttgc caagagattt
     3181 tgggcgaggc ggtcgtgcca ccgatgccga tgctgccgtc caacctgcct ttgaacgcgg
     3241 tttgcagtac gtcttccgcg ccgacggagt tgatgcctat atttaaatcg aggatgtctt
     3301 ttccggtgtc gattttgccc gacagcctga tgccgccccg tccgagcagg gcggcggacg
     3361 tattgccgat atgcaccgtg ccgtcctgcc ggatgacaaa gctgcctaaa acctgacgga
     3421 cggggatgcc gttgcggtcg gcaaaaccgg ctttggtgtt ttccaaatcg agcgagcctt
     3481 ccagcgcgat gccgtctgaa aacgacggga tggcggtcag gtcgaaattc agcccggcat
     3541 cgggcaggga aggtacgaag gcggccggat tgatgttgaa ccctttgacc agtacttctt
     3601 ccaatgtttt atccaatgat tcggcaaacg ggtggatgac ggattttccc gagaggcgga
     3661 tattgccgcc gtcgatcgcc agttcggcgc gcacatcctt caggctgccg ctcagccgag
     3721 ccgtactgtg tatggttttg ccttcgagtc cgcctttggt gtaaatggcg gtatcgaggg
     3781 caaacggttt tttcaagccg accgaggccg cccccgacga actgctccac ggcgtgtcgg
     3841 cggccttcag gtcaaggcgg tgtcctttgc ggtcgtaacg gtatgaagca tccagccgtt
     3901 cgagatagac ggtttgtttg tcaaaggctt tgcccatgct gattttgccc gtctcgaagc
     3961 ggtcgagata gacggcggca ggcaggtcta tgctgtcggg aaggctgagc ggcgggcgtt
     4021 cttctttagg cggagtcggt ttggtaacga tggcgatgtc gccggcggaa atttcggtaa
     4081 tgtgcaggct gcggtgcatc agttcggacg gtttccacgc gaagcggaag cggctgattt
     4141 taaggtctgc cccctcggtt tctatcgacc agttgtcgcc gtcgaagccg tcgagcagcg
     4201 tgcctttgag attttgggag gaaatgttta cgccgaacca agacgggatt tggtacagcc
     4261 cgaagcgcaa acccgcttcc gtaccggcga gccagccgag gaaacatact gccaaaatca
     4321 ggacagacag cagtgccgcc gacagcttca gcagcgggcg gcgttttttt gccggcgggg
     4381 tggggcggtg ttcagacggc attttgcgcg tgccgttttc ggtcggatcg gtatctgtcg
     4441 gtgcggtatc ggtcataatg tctgtttcaa aatagtttgc tgcaatgccg tctgaagcgg
     4501 ccgtatcggt ttagaagcgc gttcccaggc tgatgtgcca gcggattttc ttgtcgctgt
     4561 gcccgtaggc gatgtcgaag gaaaacggcg cgagtgggct gaaccagcgt acgcccagtc
     4621 ccgaaccgtg tttcagcttc atacgtttga aattggcggc ggcatcgccc atatcgtgga
     4681 acaccgcgcc ggaaagggtg cgcgtaaacg gtagttggta ttccaggctg cccaccaaga
     4741 gggcgcgttc gggtaggacc gatccgttcg ggccggcaag tccgatgctg tcgagttcgt
     4801 aaccgcgcac ggaagacgcg ccgccgctgc ggaacatcag tcccgaagga acgtcggcat
     4861 tgtcgcgggc aacggtgtaa cccgcttgtc cgcgtatgat gaacgtgccg agtttcttgt
     4921 tttcgggcgt gaagaaataa cctgcgcggg cagaggtgcg gatcagcgcg gtggaggaca
     4981 ggaatgtgcc caaagtcgta ccgattttgc cgtcgaggta atggccgttt tcggggtgca
     5041 acgcgttgtt gagcagctgg cgtttccaag aggcggtcag catcgtggcg tggctgttgc
     5101 ccaaatcgac atccgagccg gggattttcc ggccttctgc gagaaactcc gcccccagcc
     5161 tggcatcaat gcccgcgcgg tcgcgcacat accagacgcc gccggagaag gcgcgttttt
     5221 cgaggttttg ggtggtcgaa cggttgtagg aaacgttgct tgtccagtag ttgccccgat
     5281 agttgcgcgg ctggctgatg ccggcggcaa gcgtggtttc gtatttgtcc atatcccaga
     5341 cgaccgaacc gatatagcct ttgttgaaga ggttgtaata gtcgtaggcg attttgctgc
     5401 ccaaaccgta ttccgaatcg aggcggatgc cggtttcgag tttgtggcgt ttgacctcgg
     5461 ttacgctgac tttgacgggg acgcggtcgc cttggaggcg gtcgaagtcg gcctgtacgg
     5521 acgcgccgga ataatgcccg ttttgttcga gcgcctgttg gaagtcgagc agcaggtcga
     5581 ggtcgtaggg cgtgcccggt tggaagcgcg ccaggccgga gacgatttgt tcggggtaac
     5641 gctgcgtgcc ggtaatttca aagtcgccga aggcgatggg gcggccgctg tccacgacga
     5701 cgttcaaatc ggcggtggcg gtatcggggt tgacggccgc ccgggtgttg ccgagcttgg
     5761 caagcgggta ggctttgcgc gttaccgcgc cgaggacgga agttttgctg ttttcccaac
     5821 tgtcctgatc gaaatcactg cctaccggct gctgccagtt ttccagcgcg ttgcggtagt
     5881 attcggcgag gttgccgtct gaaaggatgt cgccgaggat ggcgacgccg acgttggcga
     5941 ttttggtgcg cgggcccggt gtgatgtgta ccgtataagc tccgtctttt tccgtcaggc
     6001 tgattttgct gctgaaatag cctttgctgc ggagcatcgt tttaacgttg tccgatgctt
     6061 cttcggcgag gaagcccgtc tgttctttgt ccaatacttc ttcctgctgc tgcgtgatga
     6121 gcggcaggtg ttcttcgacc atatctttga tttcactgtc ctgcgtgtcg atgcggacgg
     6181 ggaatttggg ttttaatttg actgattcgg tgtcggggct tttgtttttg aacaatgcga
     6241 aacccgccgc cttgttttcg gaaaggtcgg cggcaggcgc gtatgcgtgc ggaaagaaaa
     6301 ataaagccgg caggagcagg gcggtcggtt taatcatcat ggtacgggta tcgtgcatcg
     6361 gtgtggcgga tttcgattct aacactgttc ggacggggcg ggacgcttca gacggcatac
     6421 tgccgccggt atttcggagg ttgcggcccg ctggaaatgt atctgcttgt tttaaggtat
     6481 tttgtttcta atccgcatat ggaaaacagg ctgcgtttcg gaatagtgtc ggaaaataaa
     6541 cgggattaaa taaatttatt ttcgttcaat aaattaaacc gtcgcgccga atgataaagc
     6601 aaagcctgtc cggcactgca tagggcaaat caacgcaacc ttcatagtgg attaacaaaa
     6661 accagtacgg cgttggctcg ccttagctca aagagaacga ttctttaagg tgctgaagca
     6721 ccaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcatgatt
     6781 tttgttaatc cactataaaa cacagccgaa aaataaaaaa aacacagaga atgagttttc
     6841 ttttaaaata caacattttt taacatatca caacataagg aagaattatg gcgttcggca
     6901 aatccttatt tcatgcgatc ggccgcgtca gcctggtcag gcagattgcc gccggtttgg
     6961 cgttgggcat cgtaatcggt tcggtttccc cgcaactggg cttggcggca ggcttgttcg
     7021 gcagcctgtt tgtcggcgcg ctcaaagcgg tcgcgccggt tttggtattt attttggtgg
     7081 cggccacaat cgcgcagcac caaaaaggca acaaggcgca tatcaggccg attatcatcc
     7141 tttacctcat cggtacgttt tccgcagccc tgaccgccgt catcgccggt atggttttcc
     7201 cgacgcacat tgttttggcg ggcgcgggcg atgtgtccgc cgcgccgcct tccggcattg
     7261 tggaagtatt gaaatcgctg ctgatgaacc tggtcgccaa cccgattaac gcgattgcca
     7321 atgccaacta tatcggcatt ttggcttggg ctttggtttt gggcgcggcg ttgcggaatc
     7381 acggttcgga cgttacgcgg caggtcgttg ccgatttggc ggaagcggtt tccaccgtcg
     7441 tgaaatggat tatccgtttt gcccctttgg gcattttcgg gctggtgtcg tccacaatcg
     7501 cggagacggg tttcggcgcg ctggcgggtt atgcgaagct gcttgcggta ctgttgggct
     7561 gtatggcgtt tatcgcgctg gtggtcaatc ccgccatcgt gtggtggaaa atccgccgca
     7621 acccttatcc gctggtgttt acctgcctgc gcgaaagcgg cgtgtatgcc ttctttaccc
     7681 gttcttccgc cgccaatatc cccgtgaata tggctttggc gaaaaaactg gggctgcacg
     7741 aagacactta ttcgatttcc atcccgttgg gggcgaccgt caatatgggc ggcgcggcga
     7801 ttacgattac cgttttggct atggcggcgg cgcacactca aggcatacag gttgattttg
     7861 ccaccgcgct gctgttgagc ctggtggcga cggtcagcgc gtgcggcgcg tccggtgtgg
     7921 caggcggttc gctgctgctg attccgctgg cgtgcagcct gttcggcatc agcaacgatg
     7981 tcgctatgca ggtggtcgcc gtcggcttta tcatcggcgt gattcaggat tcggcggaaa
     8041 cggcgttgaa ttcttcaacc gatgttttgt ttaccgctgc cgccgatttg ggtcgacaga
     8101 gaaaccgggc ggaataaacc aagctatgcc gtctgaagcc cgtttggcgt tcagacggca
     8161 tatttttaca attttatggg catatggcgg aaacaatacc ggctgccttg aatatgaagg
     8221 ggatgatgat gtttaaacgc agtgtgattg caatggcttg tattgttgcc ctttcagcct
     8281 gtgggggcgg cggtggcgga tcgcccgatg ttaagtcggc ggacacgccg tcaaaaccgg
     8341 ccgctcctgt tgttgctgaa aaagagacag aggtaaaaga agatgcgcca caggcaggtt
     8401 ctcaaggaca gggcgcgcca tccacacaag gcagccaaga tatggcggca gtttcggcag
     8461 aaaatacagg caatggcggt gcggcaacaa cggacaaacc caaaaatgaa gacgagggac
     8521 cgcaaaatga tatgccgcaa aatgccggca atacggctgc ccaaggtaca aatcaagccg
     8581 aaaacaatca agtcggcggc tctcaaaatc ctgccccttc aagcaatcct aatgccacga
     8641 atggtggcaa ttttggaagg gttgatttgg ctaacggtgt tttgattgac ggaccgtcgc
     8701 aaaatataac gttgacccat tgtaaaagcg attcttgtaa tggtgataat ctattaagcg
     8761 aagaagcacc gtcaaaatca gaatttgaac aattaagtga tgaagacaaa attaagaaat
     8821 ataaaaaaga tggagaaaag tttaccggtt tggttgctga taggttacag atgaaaggaa
     8881 ccaatcaata tattattttt tacaaaccta aaaccacttc atctgcgcga ttcaggcgtt
     8941 ctgcacggtc taggaggtcg cttcccgccg agatgccgct gattcccgtc aatcaggcgg
     9001 atacgctgat tgtcgatggg gaagcggtca gcctgacggg gcattccggc aatattttcg
     9061 cgcccgaagg gaattaccgg tatctgactt acggggcgga aaaattgtcc ggcggatcgt
     9121 atgccctcag tgtgcaaggc aaaccggcaa aaggcgaaat gcttgctggc acggcagtgt
     9181 acaacggcga agtgctgcat ttccatacgg aaaacggccg ttcgtacccg accagaggca
     9241 ggtttgccgc aaaagtcgat tttggcagca aatctgtgga cggcattatc gacagcggcg
     9301 atgatttgca tatgggtacg caaaaattca aagccgccat cgatggaaac ggctttaagg
     9361 ggaattggac ggaaaatggc ggcggggatg tttccggaag gttttacggt ccggccggcg
     9421 aggaagtggc ggggaaatac agctatcgcc cgacagatgc ggaaaagggc ggattcggcg
     9481 tgtttgccgg caaaaaagag caggattgat gttgatgccg tctgaagcga gggcgttcag
     9541 acggcattgt gatgtttggg tcgcgggtcc ggttcaagtc cgcatcagtt gataaacccg
     9601 tcgtctttca ttttcttttc ggaaaaatat tgaccgcata ccagtaaggc ggcgaggatg
     9661 atgacaaaca gccccgccat agtcagtgtg attttgaaga acaattccgc ttcgagaaaa
     9721 gaaaaccaaa gttgcacaat cagcagcagc acccagccgg caaacagtcc gagacaggca
     9781 taaacgccga gcttaaacac ggtcccgctc caaaatgacg acgtaggcct cgcgtttgaa
     9841 aaacaacagc atacggcgca aatctttttc catcgttaca acccgccagc cttcgggggt
     9901 ttgtttgttg aggaacgcgc tgaaattgac ggggttgacc tttgccgcgc cgaaaaacag
     9961 gctggacaac aggctttcct gataaatgac gactttgtat tctttcatag gattcctttt
    10021 aacataaggg atcatctttt cagacgaccc cttaagttgc taatgatgca aaacagcaaa
    10081 ccttatttct tattgcccaa ctgcggcaat accgaacctt cgcccaatgc caacaaaccg
    10141 ctttgcgagt aaatgcccag tttggcgcgg gtgtcggtaa tgtcgaggtt gcgcatagtc
    10201 agttgtccga tacggtcgag cggtgtgaac gctgcgtctt cgactttttc catactcagg
    10261 cgttcgggtt ggtaggtcag gttgggcgat tcggtgttca gaatcgagta gtcgttgccg
    10321 cgacgcaatt cgagggttac ttcgccggta atggctttgg caacccagcg ttgggcggtt
    10381 tcgcgcaaca tcagggcttg gctgtcgaac cagcggcctt ggtagagcaa acgtccgagg
    10441 cgcaggccgt tgatgcggta ttgttcgatg gtgtcttcgt tgtggatacc ggtcaccaaa
    10501 cgctcgtagg cgatgtggaa caacgccatt cccggggctt cgtagatgcc gcgcgatttg
    10561 gcttcgatga tgcggttttc gatttggtcg ctcataccca gaccgtggcg gccgccgatg
    10621 cggttggctt cgaggaagag ttcgacggga tcagcgtatt ctttgccgtt cagtgcaacc
    10681 ggcacgcctt cttcaaagcg cacgctgact tcttcgggtt tgatttcgac gttttcgtcc
    10741 cagaaggcaa cgcccataat aggtttgacg attttgatgc ccgagttcaa aaattccaaa
    10801 tctttggctt cgtgggtcgc gccgagcata ttggaatcgg tggaataggc tttttccacc
    10861 gacattttgt agttgaaacc gttggcaatc aaaaattcgc tcatttcatg acggccgccg
    10921 agttcgtcga taaattgctg gtcgagccag ggtttgtaga ttttcagcgc gggattggtg
    10981 agcaaaccgt agcggtagaa acgctcgatg tcgttgcctt tgtaggtgct gccgtcgccc
    11041 caaatattca catcgtcttc tttcatggcg gaaacaagca tagtgccggt tacggcgcga
    11101 cccagaggcg tggtgttgaa gtaggcgatg ccgccggtgg aaacgtggaa cgcgccgcat
    11161 tggatggcgg cgatgccttc gtgtgccagc tgtgtgcggc agtcaatcag gcgggcgttt
    11221 tccgcaccgt attccattgc ttttttggga atggcattgt agtcgtcttc gtcgggctgg
    11281 ccgaggttgg cagtgtaggc ataaggtagc gcgcctttga gtttcatcca caacagcgcg
    11341 gcagaggtat caagaccgcc ggagaaggcg atgccgactt tctgaccgac ggggaggttt
    11401 tgtaaaatag tatggttttg gctcataagg gtatccttcg gacttggaaa aaggagttcg
    11461 tcgtgtttcc ttttcgattg aaaccccgcc acttggacat ctgtccttcg gggcggtaga
    11521 atcagacttt atttgggagg ggcgtaatcc cttccaaatc aggacggcac atagggcggt
    11581 gctttatgtg tcgtcccgtg tgttgaaaca ttatcttata aatataaccc attgtcagcc
    11641 gttaaggctg tctgaaaaga agctttgccc gtttcgctga aaatcttttc agacggtctt
    11701 tacgccatcc tcaacctgac tccctcccct gcgggggagg gttggggaga gggtgcagac
    11761 agcagccctt aagcaaacgc tgcctgaaaa acgcaagctc gaccctctcc ctaaccctct
    11821 cccatgggag agggaacaaa tcacagcacc atcgacattt tcagacggca tcggtaaaga
    11881 aacccatcta tgaacgcgtt caacctcacc atcatcggcg acgaaatctt acacggcagc
    11941 cgccaagaca agcatttcgc ctttttcaaa tccctgctcg aatccaaagg gctgaagctc
    12001 aatcaggtgc aatatctgcc cgacgaaccc gatttgctgg tcaggcaact gcgccgcagc
    12061 ttttcagacg gcataccgac gtttgttacc ggcggcatcg gcgccacgcc cgacgaccac
    12121 acgcgccaag ccgcagcggc tgctttggat ttgcctgtcg tccgccatcc tgaagccgcc
    12181 aagtttatcg aaggcgtaac ccggaaacgc ggtgagccgc tcgattcgcc cgaacacgcc
    12241 caacgcctga agatggcgga ttttccccaa ggcgcggaat tggtgcctaa tccgtttaat
    12301 aatatcgccg gattttccat tcgcgagcat tatttcttcc ctggttttcc tgtgatggcg
    12361 cacccgatgg cggaatgggt tttggatact tattacgtcg gccgcttcaa ccaaaccaaa
    12421 cgcggcagcc gcagcgtgta tgtgtttgag cagcccgaat cgcgcattac gccgcttatg
    12481 gagcatatcg agcaaactta tcccggtgtg cgttcgtaca gcctgcccag cgtcggttgg
    12541 acgcattcag acggcacgca ggtcaaaccg cacattgagt tcggcatcaa agccgaagga
    12601 gaagccgtca accttttgga cgcggcgtgg gcggaagtgt tgcacagctt ggacggattg
    12661 ggagcagagc tgaaaaatcg ggtaaactga aaaccgtttg cccaaatcgg acgatattta
    12721 aggaaaacca tgcaataccg aatcagaaga gaaaacgaag cacccgaagc aaaaaatacg
    12781 ggggaaacct tatgggagcg cgacatcatg cgcgaagtgc tgctgtccgc ctatcaggac
    12841 aggcgcaggg agcggatgtg gaaaaacatc tggcgtgccg tcagcaccct gattctggtt
    12901 gccctgattg caggcatttt ccgaaaagac gaagccgcat tgcagttggc gggcaacacg
    12961 ccgcataccg cagtcgtcaa cctgtacggc gaaatcggca acggtgtaga agaccaggtc
    13021 aaaaaactca aagacggtat ggaggccgcc tacaaaaacc cgcaggcaaa agccatcgtc
    13081 atccgcgcca acagccccgg cggttcgccc gtcgtctcca acaccgcttt tgaagaaata
    13141 cgccgtctga aggcgcagca ccccggcatt cccgtttatc tcgtggcgga agatatgtgc
    13201 gcgtccggct gctactacat cgcggcggcg gcggacaaaa tctatgccga cccgtccagc
    13261 atcgtcggca gcatcggcgt tatcggcagc agtttcgacg cgaccggcct gatggaaaaa
    13321 atcggcgtga aacgcagggt taaaattgca ggcagcaaca aaggcatggg cgatccgttt
    13381 tcgcccgaaa cgcccgaaca gtcgaaaatc tgggaagaaa tgctgaccgg catacacggc
    13441 gaattcatca aagccgtcaa aaccggacgg ggcggccgcc tcaaattccg acaatatccc
    13501 gacgtgttca gcggtcgcgt gtacacaggc gcggatgcgc tgaaagtcgg gctggtggac
    13561 ggactcggca atatttacag cgtggcgcga gacgtggtca aagcccccga tgtggtggat
    13621 tacactccga aggacgattt cggcagaatc ttgggcagac gcttcggcgc agaactcaaa
    13681 gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt gtttacagta tttctaaggg
    13741 aataccaaaa taaaaatata cacaaaaaca ctcacctccc cagtatgctg ccactaagtc
    13801 agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag gcaggttgtc cccgggaggt
    13861 tttgtgcatc ctgataccga tcggttgttg ttgctcaaag gacagaaggc cgctgataaa
    13921 cgagattacc tgtttgtcgc tattgacgat ttttatactc tgccattttg ccagacaaaa
    13981 ccgcagacag tgctgccaag tttctgaccg aacatctggc cgacccctgc ttgtacctga
    14041 ttgagtacgt ttactctgac aatgataggt aatataaaga gccgtccaac atgctttcgg
    14101 tgcagcttgt tataataatg ggattggttg gaggcttgcc cgatttgctt gtccgcagac
    14161 caacggtaag gaggagcggg ttatccgtac cttgatggag atgtggcatg aggaacagtc
    14221 gtttgacagt ccggaacatc ggcaaaagga gttgtgctgc tttattaatt taggggctac
    14281 cctagatcac tagggaaatt caaattaagt tagaattatc cctatgagag aaagtcgtct
    14341 aagccggtat aaacaaaata aactcatttt gttatcgtgg ctatgatgta ttggatgcgg
    14401 gcgaatttag ccatttccgt atcaatcaca gcacacattt tgccgaacga caaaaccata
    14461 ttaatggaac tgagaacttt tggaatcaag caaaacgtca tttacgcaag tttaacggca
    14521 ttcccaaagc gcattttgag ctgtatttaa aggagcgcga atggcgtttt aacaacagtg
    14581 aaataaaagt tcttgttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc
    14641 cagggcagcc tcatctcttt atagatagct gcttaccaag tatccttaat tgctaattca
    14701 agccaatctt caaaactatt aaaaaattca tggttttcta tattatctaa attaatgaca
    14761 ataaccctag aatttcctgt tacatcatta ccatcaaaat aagcttccca ttctccatta
    14821 ctagcgaatg ggataagatt taaatcaggc aggatagaat ttctaaatat ataagctact
    14881 tctgcccctt ctgttccata attctcaaac caccagtgaa aattatgtgg ctgaatataa
    14941 ggtattaaag atgtatcgtt agagatacga acatactttt caggataaca aaacctttca
    15001 ggaagcatat tactaggata taaattaaat tcattttgaa taatcatttc gttaccttag
    15061 gatcataaat actaccatta ggaaactggg tagaccaatt tggtggtgct agttcatttt
    15121 caatttgttt tttaattcta aattgaatag cttctcccca tgtttgcggc atctaatgct
    15181 gcttgctctg catcaatatt actaaaaatt ggattagtct caaaaatgaa aaattactgt
    15241 tttttcattt tttatagcat attcaagtac atgaataata tttttcacta agggctctgt
    15301 agaaaaatct gaattttgta atttttcttt tacagccaaa atactttcct tatctattag
    15361 ccaatccccc tcacttggtc caaataacca attatcggta atttcagaaa caagtctcca
    15421 taatccacga ttttgatatt caataatttg actttccgta atttctacaa ctgtttcaga
    15481 ttgaatgttt ttataagccc aatcaatcat ttcagtatat tcactaattg aaacattgtc
    15541 ttttaaacag aaaattaaca ggttgtgttc attcatttgt ctaagttctt aatatgagtt
    15601 tgttgttgta aataatcttt tgcttgctta ccttgtaaat taaaggatta accgcacctt
    15661 ggatgatgcc gcgaatatca tccatacggt taccccacca aattttattg gtgtatcgtt
    15721 aatcatttat ttcaaaaaag ttatttatta tcatattatc agggagcagg tcgttaaaaa
    15781 aaaccccctc aaattccatt aaattagtat ttatccagca cataaacgaa ccaaagcgtt
    15841 ctttccctga gtgtaaatat ttaaaattaa actcaaaata agtttttttg cttagaaaat
    15901 ttaatggttt ttctatagtg aaaaaaagcc cttactcgtt ttagttcgag atactatttt
    15961 aaataaattt ttattttcaa taaaataaga atattcattt actaagttgt tatgataaaa
    16021 aagtaatttt attaaaatgt tttcataatt ttctaggttc atttctagct caatttaaaa
    16081 tgatttcatt gttgccgtac caatgcgaaa tttaccatca acaaaatttc ccttcactga
    16141 tacaggtacg ccatcaactt gggtagtttt attttcaggc tgcctgaaag aaagataggc
    16201 aacttattac tcatcttacg ctagccctca ataattgaat acgttgcact agaaccaatt
    16261 atttctatga atgtttgctc aaacataaaa atatagtatt taaagaattc tctattcaaa
    16321 ggaaaataac tttctttcaa tatccattcc gcataacact catcaagaag ttcaaaaaag
    16381 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
    16441 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
    16501 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
    16561 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
    16621 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
    16681 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ctatcaggta
    16741 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
    16801 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
    16861 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcacct
    16921 taccggtttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
    16981 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
    17041 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
    17101 agccggtatt tttaacccaa tcccatttag tcgggttaag ttctactttt ttacctctcc
    17161 aaaccgtacc tgcggcctct gctacggaaa gggcagtttg ggctgttgca gttatattcg
    17221 gatgggcatc agcccattgt ctggcggaat tgatgccgtc ttttaccgca aaagcactgt
    17281 cttgtaatgc ggttgcggcc gcaagttgtg cttcgggact taaatttcct aaattattaa
    17341 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
    17401 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
    17461 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
    17521 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
    17581 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
    17641 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
    17701 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
    17761 atatccagaa tgatgacgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
    17821 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
    17881 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagcctcatc
    17941 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
    18001 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
    18061 cataggtcgg gcagcaggtt ttttatagcc ggtattttta acccaatccc atttggtcgg
    18121 gttaaggttt acttttttac cgccccaaac cgtacctgcg gcctctgcta tggcaagagc
    18181 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
    18241 gctgtctttt accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc
    18301 tggacttaat tttcctaaat cattaatacc ttgtagagtc tgctgagcgg ctgtatctga
    18361 gccggtcagt gtaggttgga ttaaaaccca gcattttcaa catcttgatg ttttactata
    18421 aatctattct tgtgcttcct ttgttagata aatttcaaca caaaagatac gtatgcattc
    18481 tctaatttta tttgagataa ataatgcatt gctttgtcga tactgttaaa taaattactt
    18541 ccctcgtaat accaattatc atatgtttga taaaaataat tatctttctt gatataaata
    18601 tctcccccca atacaagtat tctctcattg ataaataaat ttaatatatc tattgcaata
    18661 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
    18721 taaatttcaa aaatttcatt taacatgtta atctctatct actatttttc aatcggtata
    18781 aaccctattc acattttatt tttcctaaaa ccaaatcttc gactaactgg gttgcataaa
    18841 taatatcatt tatgatttta acaggtaaat tatttgatag gtaaccatgg gattccgtca
    18901 tcattgattt ctctaggtgc ttatttatct gattggtaag ccttttgcgt aagcttcttt
    18961 cgcctccgca agtgtcattt cattaggtcc accatctata ttagtttctc ctgtattttg
    19021 ccactgacaa acgtcacaga tatcatagtc cataacttca gttccgcaaa caggacaatg
    19081 tagccatatt tctccgtcaa tttcccatgt cttttttgaa ttctccatca taatacttct
    19141 ttcctaagtt tggtttaaac attgttttga ttttgaatgt ttttggattg gccaaaacat
    19201 agatattgtt attgatatca tagcgcactc gtctatgttc cgtctgataa cctaatatct
    19261 tgtccgatgt ctgttttgat aacaaatcag acgccatttt ttggtagtct tcaatggtta
    19321 tatcgccaaa ttcttttcca tgagctttga aatgtccatt taaagatttc tcagtaggaa
    19381 acttggactt tgtccatctg atgcggtctt gaattgttcc tgtcggagaa atagatgtat
    19441 caggtttaac atatgaggca ggtaagccga tattgttatc agagtgtgta gggtgttgct
    19501 gcgtacttgg ttttggtggc ttattcccac ctgccatctc cccatctaaa gtctgcatag
    19561 ggcgggcagt aggtgtttta tagccggtat ttttaaccca atcccatttg gtcgggttaa
    19621 gttctacttt tttaccgccc caaaccgtac ctgcggcctc tgctatggca agggcggttt
    19681 gggcagtagc tgtaatgttc ggatgggcat cagcccattg tctggcggaa ttgataccgt
    19741 cttttaccgc aaaagtactg tcctataatg cgcttgcggc agcaagttgt gctttcggac
    19801 ttaaatttcc taaatcatta ataccttgta aagtttgctg cgcagagatg taggtcggat
    19861 atttgtatcc gacaaaaata tttgacgcat ctatcgtttc cgaaatgccg ctgttggaaa
    19921 atgtcggatt cgagaatccg acctacggct atttcgttta agtcataaat agccattcat
    19981 gttccttctg aatttgcttt tactatcctt agaaccaatc atataaacat tatcatcatt
    20041 aattaatgtt ctaagtttat gaagcggtca aatctgtcaa actaccagag atgaagcata
    20101 tcctcctgga agtaattctg ttcgtgctgt ttccatatct tctttgccat catcagttac
    20161 ttctctgtct aaataaaatc ttacgataag tttttgtcta atttcatcaa attctgccgt
    20221 aattaatctt atattgatat gaatacacgg ttcatacctg ctattagcca gtcaggtgta
    20281 gttggatggg gattcatttt taataatatc ttcaagcaag tgaaatgtaa gaaaagcgta
    20341 gatacttgaa ggccggctat ctgattggca aaaagaaatt tcggagataa agaatttccg
    20401 tttatatttt tcataaatta aatcaacacc ttgttttaca taaaaactaa cttcctcagg
    20461 aaggatattt ataacattct ctccttttac ttttatagtt aatggtttga tttttatgtt
    20521 ttcatgaata tctgatagga ttatttctaa aaaattattt ttatttccag tcattctggc
    20581 tattttatgt atatttccat tgtgttcgat tatcatttta ttttttccaa ggatttcctt
    20641 ctatattgat agtttatcta aagatttcat ttaaaatatc ctgactaatt tcttgtttat
    20701 ataattctac aattttctgt ttctgtgtat ttgtataaaa tttttcgttg gtatctgctt
    20761 gatattttaa ttgtctcaat atttccatga tttcatattc tgatttttca tcatattctt
    20821 caatcatgta atcaacaaat tgttttagaa tatcaaaggc ttcgtaactt tccaaaattt
    20881 taatatgttt atcggcaaaa ttggacatat accattcgtc agtgattgga ttagtattta
    20941 aatcatctaa aaaaacattt acatcatcta aaaacatgac taatccttaa acttaaaatc
    21001 tgttttctca ccggttcttg agttttcaat gtaatgtatt tcaattttgt ttacgttttg
    21061 aacacgctta acccaaccgt cttttgctaa ccaaccattt ttagtatcgg acataggagg
    21121 tattctcgca ggagttttgc cctgtgggtt tgccttaact tcttccattg ctaattgttc
    21181 ctttaaatta ttaggtattg tacgaccggt tgatttttca acagattgtg tccctatttt
    21241 taatgatttg tttcccccag ccatttcccc atctaaagtc tgcatagggc gggcagcagg
    21301 ttttttataa ccggtatttt taacccaatc ccatttggtc gggttaagtt ctactttttt
    21361 accgccccaa accgtacctg cggcctctgc tatggcaaga gcagtttggg ctgttgcagt
    21421 tatattcgga tgggcatcag cccattgtct ggcggaatta atgccgtctt ttaccgcaaa
    21481 agcactgtcc tgtaataggc tcgcggcagc aagttgtgct tccggactta aatttcctaa
    21541 ttcattaata tcttgtaaag tttgctgcgc agagatgtag gtcggatatt tgtatccgac
    21601 aaaaatattt gacgcatcta tcgtttccga aatgccgctg ttggaaaatg tcggattcga
    21661 gaatccgacc tacggctgct accaaccatc attttcagct tcttttagcc aagtatcaaa
    21721 attttcaaaa gtctcgtaat attttgtatt atctaaatta actactataa ttttaggatt
    21781 acctgattta tctgaaatat caaaacaggc agcccactct tggtttctag caaaaggtaa
    21841 taggtttgga atgccagtta tttcaaaata aatgtcaatt acttctgata tattgctttc
    21901 tgcattttca aaccaccaag gaaaagaata ttgttcatcg tagttaatgg catgagtgga
    21961 ttgagctaat tttaaataac tttgcggata tttaaatcct ttaggaagca ttgcttcagg
    22021 atacggaaca aaactatttt gttttatcat ttcctaagta cctttacatc ataaatactt
    22081 ccgttaggaa atcttttgct ccacccttct ggaactcctt tttgtgtttc ttgctttcta
    22141 attcgtagct ggatagcttc tccccaagtt tgaggctcaa taccatcatg cttaaacctg
    22201 attctctctt ttgatgataa tcggtcatta aaattatgaa cattcgcttc cacttttact
    22261 cctgcttcac gtgctgccat aatacgcgtg ttatccatac tagtaggtgc tccatcaggc
    22321 atattaacaa catcaaccct gtcgccaacc catgattttt ttctttcatt tcggctacaa
    22381 tagaatcata gttgtaattt gcactaggct ctgacaaaaa tttgggttgt taagttgtca
    22441 tttttttata aattccagca aactgttgtt gtaaattatc tacttcatgt tttctaacac
    22501 cagatacatt ttcaaattct cttgaatcta cacatacgcc cgcatatatc tcattcaaga
    22561 tagctcgtaa aacaaaaact tcatcttgag ttagtgaaat agttgagttt tgattattta
    22621 tggatagtat attcatggct ttttcctata aatcatcata atatttttta cctgatgttg
    22681 gtctaaatgc tgtacctcca tcgtcagaat ttttatctcg gataactatt gtccctgttt
    22741 tatcatccca atacgcagtt ctaccgcgag gtaactcttt catatttgtt ggatggctaa
    22801 caatattttc aatatgccga gcaaaatctg ctggtgaatt gatatttaaa tccgtaaatt
    22861 cttgttgtct tatgacatgc ttgttataag catgcccact actaatttgt tctccaatta
    22921 gttgcgcttg taaagacggt tgtgtggaag catctgcttt gctgttggac gttatagatt
    22981 taggcggtct attcccaccg gccatttccc catccaaagt atgcatggtg cgaacagcag
    23041 gtgttttata gccggtattt ttaacccaat cccatttggt cgggttaagt tctacttttt
    23101 taccgcccca aaccgtagtt gcggcctctg ctacggcaag ggcagtttgg gctgttgctg
    23161 ttatattcgg atgggcatca gcccattgtc tggcggaatt aatgccgtct tttaccgcaa
    23221 aggcactgtc ctgtaatagg ctcgcggcag caagttgtgc ttccggactt aattttccta
    23281 aatcattaat accttgtaga gtctgctgag cggctgtatc tgtgacaggg cttaccgcac
    23341 tgtctgtaat tgccccaatc cctactcctt gaaaacccat taaaaaagga ttaaccgcac
    23401 cttggacgat gccgcgaata tcatccatac ggttagccca ccaattttta ttggggtcgt
    23461 tttcccatat cagttttcct gcttcatcag cacagttagg attggcattg atgcccaccc
    23521 agtttcaggc agcctgaaaa ggatttttaa tatacggcaa aaggtgggca acaagtgccc
    23581 accttaccta gctaacattt atcccaagta tcatatattt ttttaagttc tttatccaaa
    23641 gaaagaactt tattttttca acaccatgaa ttatctcaaa atctctacaa tccacgcata
    23701 cgcctgaata aatttctcct acaattgctt gaataatata caactcatct ttatcgagac
    23761 taattttaac tgaactctct tcaaaattaa ttaatttcat gaataattcc ttttatttta
    23821 attcgtcatc aaaataccgt ttgcctgttt ttggtctaaa tgctgtccac catctttagt
    23881 cttggatatt tagattataa tcgtcaaatt cccagtcaaa atattttgcc aaatttgata
    23941 tagacttatc ttcagacgtt gaattacata tttccaatat atcatttatc aattttaata
    24001 tgtgttcaat attaaaacgg tttgtaacaa ttgagttttt taatgacatc ggtctatgta
    24061 tatttgaaag taaccattca gtgctatata tataaacata aaaataatca gcaccattac
    24121 tttcatcaaa acctattgat aatgtcaaaa gtatacaaaa attttctaac ttgtcttgtg
    24181 ggaaatcttc caaatcaaag tcagaacaat cataattttt taattttaat ctcatataat
    24241 tacctaatat ttaaatgtcc atttttaatt ttattccttt ttccatttga atcaatagta
    24301 taagtttcaa aatttgcttg aatacctgta gttgcaaatt gtgaattttt ttctgttggt
    24361 ggccgatatt gtcgagtgcc atctatactt aaccatccgc ctccactagt ttgtcttgca
    24421 ccctcaccaa cccaaatttt acctagccta tctgcctctt cataagttgc agttcctatt
    24481 ggaaaatttt tttcactctt atgaagagct agactcaatc ttggatcttg agccgcaatg
    24541 ttttttaagt tttgcccagt taattgatta actaattgag gataagttgc agcattggct
    24601 tttcctccag acgttataga tttaggcggt ttattcccac cggccatttc cccatctaaa
    24661 gtctgcatag gtcgggcagc aggtgtttta taatcggtat ttttaaccca atcccattta
    24721 gtcgggttaa gttctacttt tttaccgccc caaaccgtac ctgcggcctc tgctatggca
    24781 agggcagttt gggctgttgc agttatattc ggatgggcat cagcccattg tctggcagag
    24841 ttgatactgt cttttaccgc aaaagcactg tcctgtaata ggctcacggc ggcaagttgt
    24901 gcttccggac ttaaatttcc taaatcatta ataccttgta gagtctgctg cgcagagatg
    24961 taggtcggat atttgtatcc gacaaaaata tttgacgcat ctatcgtttc cgaaatgccg
    25021 ctgttggaaa atgtcggatt cgagaatctg acctacggct gctgactatt ttcttgttta
    25081 tataattcta caactgcaaa ctactatcaa atctttttat ctctggctct acactcagca
    25141 agaatatttt tattttacaa aacataggct agttatttga gtatattgtc aaattctaat
    25201 ttgttttttt gatagatttt ttttatacca ttaatacatt cattagttgt aattctacca
    25261 agcgaatgta atttatcaat atcaaaacaa tcggtataaa ccctattcac agttttatct
    25321 tgtatttcct tatgaatact taatatattg agatagttta gaaattttat tttctcagat
    25381 tgaggtaact catcaaaacc aagccaataa taaaaattgt tatatgattt taaaaaatca
    25441 acaattttta tttttcctaa aaccaaatct tcaactaact gggttgcata aataatatca
    25501 tttatgattt taacaggtaa attatttgat gggtaaccat ggggttccgt catcatttgt
    25561 aactcctggt acatgtgcgt gtcctgtaag tgcgcgtggg tcttgatggg tatttgcatg
    25621 atgagcatca tctattctgg ctccagtcaa gtttccatta ctatctcgga cttggataaa
    25681 acgagcatca ggcgaacctg ttatataacc gcctattggt gttggtattg catttccttt
    25741 gctgtctaca taaattgttc ctgtcggaga aatagatgta tcaggtttaa catatggggc
    25801 aggtaagcct atattgttat cagagtgtgt agagtgttgc tgcgtacttg gtttcggtgg
    25861 cttatttccc ccagccatct ccccgtctac agtctgcata aggcgaacag caggtttttt
    25921 atagccgtta tttttaaccc aatcccattt ggtcgggtta agttctactt ttttacctct
    25981 ccaaaccgta cctgcagcct ctgctacgga aagggcagtt tgggcagtag ctgtaatgtt
    26041 cggatgggca tcagcccatt gtctggcaga gttgatactg tcttttaccg caaaagcact
    26101 gtcttgtaat gcggttgcgg ccgcaagttg tgcttccgga cttaaatttc ctaaattgtg
    26161 aataccctgt aaagttttcc gtgctgccgc ataggttacc gggtttaccg cactgtctgt
    26221 aattgcccca actcccaatc cctgaaaacc cattaaaaaa ggattaaccg caccttggac
    26281 gatgccgcga atatcatcca tacggttagc ccaccaattt ttattggggt cgctttccca
    26341 tatcagtttt cctgcttcat ccgcacggct gaaaaaacca gaggcggcac cggcattttc
    26401 ttttagccaa cggtctgaaa atggggcttg agggacaata ttagtctttg tttttgttga
    26461 agttcctttg acataatagc tgtatatatc ccttgctcct ccggggggcg gataatcgct
    26521 gccttgcggc ccgtcatatc catcctccgg atgaatttcc gaccctgtcc gatgaagttg
    26581 gtaaacagta aaaccaccgt ctacaccgcc gctgaaatca gaagtgcttt ttgaatcatg
    26641 attatcgaac ggactgtgta cttcatgtcc atgtcctgaa aaatgggttt cataaccgat
    26701 aatgccttca aaccctgtcc gttcgtgtgt aataggcagt acaggactga ccgcagttgc
    26761 atcaaatgtc tggacggcgt aaacccgatt tttaacactg ccgcgagcat taccaaacag
    26821 atggtattta ccacccggtt cgtagtgttt tgcctgcata tcatcgcgta aacgggcatc
    26881 caaaccgttc gcatgactaa tggggatttg cagcaacgat attgccgctg caaacagcat
    26941 catgaatttt tgaataggca atttcatttt gttttccttg gtagcaagcg gttatggtcg
    27001 tgtgaatcaa ggttgccctt gtctatgttg tcgcactact tcatcgctgt atccataccc
    27061 ctcatgactg ttatcagtct ctacggatgg ggcggagtta cccgtatgat tgccgtatgg
    27121 tcggatatcg gagaaatcga ccattaatcc ttccgtcggt ttaattcctt tgcttacttt
    27181 atacggcccc atccacaatg cgtaattttc tttatagaca gcttcaaacg cattggtttt
    27241 tggtttgatg agcaattttt tattggttct gtctactgcg aaatattcca gttttgtttg
    27301 ggctttcagt gtttcggcat tgtataggtg catttcggtt ctgttgcgta tcgttccaaa
    27361 tacgtcgatg ttaataaaca catctgtatc ggcattggca ggagaaacaa cgtctatgcc
    27421 gcgcaggaaa aatacggttt gcaccaagtg ggaaagaaag gcagtgtcgc gagggttagt
    27481 cgtcaaggtt tcatttcgat aatcccccat cccgccaata tttaagccca gactgctcct
    27541 acttccgcta ccgtctgatt gggtgcgcga gcgtgcaggg gcattaagtg tagataaaga
    27601 agtggttaaa cctgtcaaac cgcctgatgt tgtttcagcg gtggtttcgt aacgtggata
    27661 ggtgtaatcg gtacggacgg cagggctgtt tatgtattcg ccacgaatca gtgcatcaat
    27721 ggagtagcga cctcctgtca aactgcctga accttggtcg cccatagtgg caatgtacaa
    27781 tgcaactttt cgtccgtgta atgcctgtaa atccatgtct ttaacggcag ctctggcaga
    27841 agcggccaca agttcttgtt cgaccgcaaa gcgtttacct ccgccatgcg atggaatacc
    27901 tgtcagtgtc ccgcaggcgg ataaaataaa aactgaaaaa agaataggta tcagcagccg
    27961 tgcttgcata gattttctcc tttgatgaaa aacaaattgt atcaaaattg taaatatagt
    28021 ggattaaatt taaaccagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
    28081 cactataatc tccatataat aaatggctgt ctgaagccca tatgcgacta tatctgccga
    28141 cactatggtc aatcagcagt gaaccagcgt accttcgttt tcaccggtta ctacacgttt
    28201 gagcgagcct tctttagcaa tgccgaatac tacaatattg agcttgcgtt cgcggcagag
    28261 ggcgaaagcg gtggcatcca tcactttgag gtttttcaac aaggcttcgt caaaagtaat
    28321 ggtttcgtag cgcgtggcgg atgagtcttt tttggggtct gcggtgtaca cgccgtcaac
    28381 gttggtggct ttgagcatca cgtcgcagtt catttccgca ccgcgcaatg cggcggcagt
    28441 gtcggtcgtg aagaacgggt taccggtacc ggcggcaaaa atcacgactt tgccttcttc
    28501 caaatattgg atggctttgg ggcgggcgta ggtttcagcg atttgctgca tagacagtgc
    28561 ggattgtacg cgcgctttga tgcctaaagt ttcaaatgcg tctttgagtg ccaacgcgtt
    28621 catcacggtc gccatcatgc ccatgtagtc ggcggtggcg cgatccatgc tgcctgcttg
    28681 ggcggatacg ccccggaaaa tattgccgcc gccgacaaca ataccgactt gcacgcccat
    28741 tttaacgact tcggcaattt cgccgacagt ttgaacgatg gtatcgtgat tgatgccgaa
    28801 cggatcggaa cccatcaggg attcgccgga gagtttcagt aatacgcgtt tgtatttgat
    28861 ttgctgtgtc atgggatacc ttgctttctt gaatgtcgtc ggtcggagtt tgggccgtct
    28921 gaattttcaa acggtcggaa ttacaatgat atggtgtgtc
//
LOCUS       DAWKQJ010000007        28912 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000007 DAWKQJ010000000
VERSION     DAWKQJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.007"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            221..577
                     /locus_tag="WJL20_000238"
     CDS             221..577
                     /locus_tag="WJL20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ7682440.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            568..1050
                     /locus_tag="WJL20_000239"
     CDS             568..1050
                     /locus_tag="WJL20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ7682441.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1063..1656
                     /locus_tag="WJL20_000240"
     CDS             1063..1656
                     /locus_tag="WJL20_000240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ7682442.1"
                     /translation="MASIQNLYETVIGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1646..2902
                     /gene="nuoD"
                     /locus_tag="WJL20_000241"
     CDS             1646..2902
                     /gene="nuoD"
                     /locus_tag="WJL20_000241"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ7682443.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2902..3375
                     /gene="nuoE"
                     /locus_tag="WJL20_000242"
     CDS             2902..3375
                     /gene="nuoE"
                     /locus_tag="WJL20_000242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ7682444.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3765..5066
                     /gene="nuoF"
                     /locus_tag="WJL20_000243"
     CDS             3765..5066
                     /gene="nuoF"
                     /locus_tag="WJL20_000243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ7682445.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEILKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIIMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSIGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5066..5503
                     /locus_tag="WJL20_000244"
     CDS             5066..5503
                     /locus_tag="WJL20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682446.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            5791..8052
                     /locus_tag="WJL20_000245"
     CDS             5791..8052
                     /locus_tag="WJL20_000245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ7682447.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTASRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8055..9131
                     /gene="nuoH"
                     /locus_tag="WJL20_000246"
     CDS             8055..9131
                     /gene="nuoH"
                     /locus_tag="WJL20_000246"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ7682448.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9212..9691
                     /gene="nuoI"
                     /locus_tag="WJL20_000247"
     CDS             9212..9691
                     /gene="nuoI"
                     /locus_tag="WJL20_000247"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ7682449.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9703..10374
                     /locus_tag="WJL20_000248"
     CDS             9703..10374
                     /locus_tag="WJL20_000248"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ7682450.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            10371..10676
                     /gene="nuoK"
                     /locus_tag="WJL20_000249"
     CDS             10371..10676
                     /gene="nuoK"
                     /locus_tag="WJL20_000249"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ7682451.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            10971..11843
                     /locus_tag="WJL20_000250"
     CDS             10971..11843
                     /locus_tag="WJL20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ7682452.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKPFLIKNILFIGI
                     VFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIVLETAYLKTGRSASMIAYGTIF
                     SLIAISIYILLVQI"
     gene            11844..13868
                     /gene="nuoL"
                     /locus_tag="WJL20_000251"
     CDS             11844..13868
                     /gene="nuoL"
                     /locus_tag="WJL20_000251"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ7682453.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLTSPVLYLAAAGVAAAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            14052..15215
                     /locus_tag="WJL20_000252"
     CDS             14052..15215
                     /locus_tag="WJL20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7682454.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            15325..16821
                     /locus_tag="WJL20_000253"
     CDS             15325..16821
                     /locus_tag="WJL20_000253"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ7682455.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16831..18276
                     /gene="nuoN"
                     /locus_tag="WJL20_000254"
     CDS             16831..18276
                     /gene="nuoN"
                     /locus_tag="WJL20_000254"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ7682456.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            18388..18687
                     /locus_tag="WJL20_000255"
     CDS             18388..18687
                     /locus_tag="WJL20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ7682457.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18796..19692)
                     /locus_tag="WJL20_000256"
     CDS             complement(18796..19692)
                     /locus_tag="WJL20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ7682458.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19676..19900)
                     /locus_tag="WJL20_000257"
     CDS             complement(19676..19900)
                     /locus_tag="WJL20_000257"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ7682459.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(20161..21084)
                     /gene="rsgA"
                     /locus_tag="WJL20_000258"
     CDS             complement(20161..21084)
                     /gene="rsgA"
                     /locus_tag="WJL20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ7682460.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(21089..22945)
                     /locus_tag="WJL20_000259"
     CDS             complement(21089..22945)
                     /locus_tag="WJL20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7682461.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVLIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22971..23108)
                     /locus_tag="WJL20_000260"
     CDS             complement(22971..23108)
                     /locus_tag="WJL20_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682462.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(23108..23692)
                     /gene="ruvA"
                     /locus_tag="WJL20_000261"
     CDS             complement(23108..23692)
                     /gene="ruvA"
                     /locus_tag="WJL20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ7682463.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(23774..24100)
                     /locus_tag="WJL20_000262"
     CDS             complement(23774..24100)
                     /locus_tag="WJL20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7682464.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24154..24873)
                     /locus_tag="WJL20_000263"
     CDS             complement(24154..24873)
                     /locus_tag="WJL20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ7682465.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24870..24992)
                     /locus_tag="WJL20_000264"
     CDS             complement(24870..24992)
                     /locus_tag="WJL20_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ7682466.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(25003..25467)
                     /gene="trmL"
                     /locus_tag="WJL20_000265"
     CDS             complement(25003..25467)
                     /gene="trmL"
                     /locus_tag="WJL20_000265"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ7682467.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25532..26029)
                     /locus_tag="WJL20_000266"
     CDS             complement(25532..26029)
                     /locus_tag="WJL20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7682468.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            26085..26999
                     /gene="bioH"
                     /locus_tag="WJL20_000267"
     CDS             26085..26999
                     /gene="bioH"
                     /locus_tag="WJL20_000267"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ7682469.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            27001..27789
                     /locus_tag="WJL20_000268"
     CDS             27001..27789
                     /locus_tag="WJL20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7682470.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(27870..27971)
                     /locus_tag="WJL20_000269"
                     /pseudo
     CDS             complement(27870..27971)
                     /locus_tag="WJL20_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            27960..28196
                     /locus_tag="WJL20_000270"
     CDS             27960..28196
                     /locus_tag="WJL20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682471.1"
                     /translation="MFVNPLYADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATL
                     LALDWQGNKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            28527..>28912
                     /locus_tag="WJL20_000271"
     CDS             28527..>28912
                     /locus_tag="WJL20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ7682472.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTST"
BASE COUNT         6481 a         7067 c         7952 g         7412 t
ORIGIN      
        1 aggcaacgct gtactggttt ttgttaatcc actataccac gaattacggt gtaaaaattt
       61 atatgacctt ataaaatcaa ataagaatcg ttatcataac atgattgtat ttattgggtt
      121 tttttgggcg ttttgccgat atttaccttt taatggtttt tgaaattcgc taaaatacga
      181 aattattgta gaaattttgt taacggattt gggtgtaacc atgttgtccg cttactttcc
      241 cgtctttgtc tttatcctcg tcggcctcgc ggccggcgtg ctgtttatcc tgctcggcac
      301 gattttaggc ccgaaacgcc actacgccga aaaagacgcg ccttacgaat gcggtttcga
      361 agcctttgaa aacgcaagga tgaagttcga cgtgcgctat tacctcgtcg ccatcctctt
      421 tatcttgttt gatttggagg tcgcgttcat gttgccgtgg gcagtcgtgt tcaaagattt
      481 gggcgcgtac ggcttctggt ctatgctggt gtttatcgtc gttctgacgg taggctttgt
      541 ttacgaatgg aaaaaaggtg cgctggaatg ggaatagaag gcgttttgaa aaaaggtttc
      601 atcaccacca gcgcggatac ggtgttgaac tatatgcgta ccggttcgtt gtggccggtt
      661 actttcggct tggcctgctg cgccgtggaa atgatgcacg cgggtatggc gcgttatgac
      721 cttgaccgtt tcggcatcat tttccgtccg tccccacgtc aggccgacct gatgattgtg
      781 gcgggtacgc tgaccaacaa aatggcaccc gccctgcgcc gcgtgtacga ccagctcgcc
      841 gaaccgcgct gggtattgtc tatgggctcg tgtgccaacg gcggcggcta ttatcactat
      901 tcttattccg ttgtgcgcgg tgccgaccgc gtcgtgccgg tagacgttta tgtgccgggt
      961 tgtccgccga ctgcggaagc cctgatttat ggcctgattc agctccaaca aaaaatcaag
     1021 cgcacttcca ccatcgcgcg tgacgaataa ggagaggacg atatggcaag cattcaaaac
     1081 ttatacgaga ccgtcatcgg cgtgcttggc gatcaggcaa gcaaagtcat ttcagctttg
     1141 ggcgagatta ccgtcgagtg tctgcccgaa cactatattt ccgttatgac tgcattgcgc
     1201 gaccatgaag agctgcattt cgagctcttg gttgacttgt gcggtgtcga ttacagcact
     1261 tacaaaaacg aagtatggca gggcaaacgc tttgccgtcg tcagtcagct gctttccgtt
     1321 aaaaacaatc aacgcatccg cgtacgcgtc tgggtttcag acgacgactt ccccgtggtc
     1381 gaatccgtcg tcgatattta caacagcgcg gattggtacg aacgcgaagc cttcgatatg
     1441 tacggcatta tgttcaacaa ccatcccgac ctgcgccgca tcctgaccga ttacggcttt
     1501 gtcggacatc cgttccgcaa agacttcccg atttccggct atgtggaaat gcgttacgac
     1561 gaagagcaaa aacgcgtgat ttaccaacct gttaccattg agccgcgcga gatcacgccg
     1621 cgtatcgtcc gtgaggagaa ctacggtggc caataaatta agaaactaca ccatcaactt
     1681 cggcccgcaa caccctgcgg cgcacggcgt attgcgtatg attttggagc tggacggcga
     1741 acaaatcgtc cgcgccgacc cacatatcgg cctcctgcac cgcggtactg aaaaactggc
     1801 tgaaaccaaa acctatttgc aagccctgcc ctatatggat cgcttggact acgtttccat
     1861 gatggtcaac gagcaggcgt attgtttggc agtagaaaaa cttgccggta tcgatgtacc
     1921 tatccgcgcc cagtatatcc gtgtgatgtt tgccgaagta acgcgcatcc tcaatcactt
     1981 gatgggcatc ggttcgcatg ccttcgacat cggcgcgatg accgccatcc tctacgcctt
     2041 ccgcgaccgc gaagagctga tggacttgta cgaagccgtg tccggcgcac gtatgcatgc
     2101 agcctacttc cgtcccggcg gcgtttaccg cgacctgccc gacttcatgc ctaaatacga
     2161 gagcagcaaa ttccgcaatg ccaaagtatt gaagcagctc aacgaatccc gcgaaggcac
     2221 catgctcgac tttatcgatg ccttctgcga acgcttcccc aaaaatatcg acacactcga
     2281 aaccctcctg accgacaacc gcatctggaa acagcgtacc gtcggcatcg gcgtcgtctc
     2341 ccccgaacgc gccatgcaaa aaggctttac cggcgtgatg ttgcgcggtt cgggtgtgga
     2401 atgggacgtg cgtaagaaac agccttacga agtgtacgac aaaatggatt tcgacatccc
     2461 tgtcggcgtg aacggcgact gctacgaccg ctacctctgc cgtatggaag aaatgcgtca
     2521 atccgtacgc atcatcaaac aatgttccga gtggttgcgt gtcaatccgg gtccggtcat
     2581 taccacaaac cacaaattcg ctccgcccaa acgtaccgaa atgaaaacag gtatggaaga
     2641 cctgattcac catttcaaac tctttaccga gggtatgcac gttcccgagg gcgagaccta
     2701 caccgctgtc gaacatccga aaggcgagtt cggcgtttac atcatttcag acggcgcaaa
     2761 caaaccctac cgcctgaaaa tccgcgcacc cggcttcgcc catctgcaag gcatggacga
     2821 aatggcaaaa ggccacatgc tcgccgacgt cgttgccatc atcggtacgc aggacatcgt
     2881 attcggggag gttgaccgat aatgttatcc gcagagtcct taaaacaaat cgacacagaa
     2941 ctggcgaaat atcccgccga ccaacgccgt tccgccatca tgggtgcgtt gcgtatcgcc
     3001 caaaccgaaa aaggctggct cgcccccgaa accatcgctt ttgttgccga ctatatcggc
     3061 atcacgcctg cacaagccta cgaagtcgcc actttctaca atatgtacga ccttgagcct
     3121 gtcggcaaat acaaactgac cgtttgtacc aacctgccct gcgccctgcg cggcggtatg
     3181 gctaccggcg aatacctcaa acaaaaactc ggtatcggct acggcgaaac tacccctgac
     3241 ggcaagttta cccttgtcga aggcgaatgc atgggcgcat gcggcgacgc tcccgttatg
     3301 ctggtcaaca accacagcat gtgcagcttt atgaccgaag aagcgattga gaagaaactg
     3361 gcggagttgg agtaggtcgt ctgaaacgac gatttaaacg taggtcggat acttgtagcc
     3421 gacagagtgg gtaaaaaggc aaaatgtcgg atttaagaat ccgccctact gaaataccga
     3481 aatgccgtca ttcccgcgca ggcgggaatc caccggtaag attcggtttc tgaatttaat
     3541 aagacattgc ttaccattga ggatggattc ccgcctgcgc gggaatgacg acagacaagc
     3601 aagtggtcga gatccaacaa aaacgattaa aggtcgtctg aaaatatcga tttgataaac
     3661 tagattttat ttcagacgac gttacaagcc ggtacacaaa gacatcttaa ggtcgtctga
     3721 aacagcggcc gcaaccgata cgaaaacaaa caggcacacc aaaaatggct atttaccaat
     3781 caggcgtgat ttttgaccaa gtggataccg ccaatcccga ttgctggaca ttggacgaat
     3841 acgtcaaacg tggcggttat accgccctgc gtaaaatcct gtccgaaaac atctcgcaaa
     3901 ccgatgtgat tgacgaagtc aaaacctccg gcttgcgcgg tcgtggcggt gcgggctttc
     3961 cgaccggctt gaaatggagc tttatgcccc gttccttccc cggcgaaaaa tacgtggttt
     4021 gcaacaccga cgaaggcgaa ccgggtacgt ttaaagaccg cgacatcatc atgttcaacc
     4081 cccatgccct gattgaaggc atgattatcg ccggttacgc gatgggcgca aaagcgggct
     4141 acaactatat ccacggcgaa attttcgaag gctaccaacg ctttgaagcc gctttggagc
     4201 aggcgcgtgc cgcaggtttt ttgggtaaaa atattttggg ttcggatttt gaattcgaac
     4261 tcttcgccca ccacggttac ggcgcatata tttgcggcga agaaaccgca ttgctcgaat
     4321 cgctggaagg caaaaaaggc cagccgcgct tcaaaccgcc attccctgct tcgttcggcc
     4381 tgtacggcaa accgaccacc attaataata ctgaaacctt ttcctccgtc ccattcattg
     4441 tccgtgatgg cggacaggca tttgccgata aaggtattcc gaatgcaggc ggtaccaaat
     4501 tgttctgtat ttccggccat gtcgagcgtc cgggcaacta tgaagtgcca ttgggtacgc
     4561 cgtttgccga aatcctgaaa atggcgggcg gtatgcgcgg cggcaaaaaa ctcaaagccg
     4621 ttattcccgg cggttcgtct gcgcctgtat tgcctgccga tatcatcatg cagaccaata
     4681 tggactacga ctcgatttcc aaagccggct cgatgctcgg ttccggcgcg attatcgtga
     4741 tggacgaaga cgtgtgcatg gtcaaagccc ttgagcgtct gagctacttc tactacgacg
     4801 agtcttgcgg tcaatgtacg ccttgtcgtg aaggtacggg ctggctctac cgcatcgtcc
     4861 accgcatcgt agaaggcaaa ggccgcatgg aagacttgga tttgctggat tctatcggta
     4921 accaaatggc aggccgcacc atctgcgccc tcgccgatgc cgccgtcttc cccgtacgca
     4981 gctttaccaa gcatttccgt gatgagtttg tgcattacat cgaacacggc gggccgatga
     5041 aagagcataa gtggggaggg tggtaatggt ggaagctaaa atttttattc tatacggtgc
     5101 agccaacaaa ggtaagagta cgacacttaa tacgcttttt aatcagattt gtcggaaatt
     5161 ttctaaattt ctagtctttt ttgaaagaca tggaaacggc ttagattttg ttgcagtatt
     5221 tgatcatgaa ggtcagagaa ttggttttta ttcatctggt gataatgaat acgaggttag
     5281 gagaaattta tacaaacttt attcgcataa ttgtgatttt atttttggca cgtcaaggac
     5341 acggggtggt agttgcgatg cagtaggatg ttatgcagag ttattgcatg gcgatgtaaa
     5401 tataattaat tggtgtgaaa agtttgagcc tacagatgaa gacaatgagc gtgctgttaa
     5461 agagttattt aagtcattta aaaatataat aaataagtta tagttttagt tggttttata
     5521 ttggttaaaa gcaaaatgct aaaaatttaa ctttgccgtc attcccgcgt aggcgggaat
     5581 ccatagtgga atctaaagaa accgatattt gaaaagcagt tgccgaaatt caaaaaatgg
     5641 attcccgcct acgcgggaat gacggcaagg tggcagtaaa cgttttaaac agtattgatt
     5701 gtcaatgaaa ctcaaaaggc cgtctgaaac ccatttttca gacgacctcc ataagattat
     5761 ttatcaaata cccgtaacta ggaacgaacc atgttacaaa tcgaaatcga cggcaaacaa
     5821 gtatctgtgg agcagggcgc gacggtgatt gaagccgcgc acaagctcgg tacttatatt
     5881 ccgcatttct gttaccacaa aaaactttcc atcgccgcca actgccgtat gtgtctggtg
     5941 aacgtagaaa aagccccaaa acccctgcct gcctgtgcca cgccggttac agacggcatg
     6001 attgtgcgta cgcattcggc aaaagcccga gaggcgcagg aaggcgtgat ggagttcctg
     6061 ctcatcaacc atccgcttga ttgtccgacc tgcgaccaag gcggggaatg ccagttgcag
     6121 gatttggcgg tgggctacgg aaaaaccgcc agccgctaca ccgaagaaaa acgttccgtc
     6181 gtcggcaaag acatggggcc tttggtttcc gccgaggaaa tgagccgctg tatccactgc
     6241 acccgctgcg ttcgtttcac tgaagaaatc gccggtttgc aggaaattgc gatggtgaat
     6301 cgcggcgaac actccgaaat catgcccttt atcggcaaaa cggtggaaac cgagctgtcg
     6361 ggcaacgtca tcgatttgtg tcctgtcggc gcattgacca gcaaaccgtt ccgcttcaac
     6421 gcgcgtactt gggaattgaa ccgccgcaaa tccgtttccg cccatgacgc tttgggcagc
     6481 aacctgatta tacaaaccaa agaccatacc gtccgccgcg tgttgccgct ggaaaacgaa
     6541 gcgattaacg aatgttggct gtctgaccgc gaccgttttg cctatgaagg cctgtatcac
     6601 gaaagccgtt tgaaaaaccc gaaaatcaaa cagggcggcg agtggatgga cgtggattgg
     6661 aaaaccgcgt tggaatatgt ccacagcgca attaaatgta tcgccaaaga cggcaaccaa
     6721 aatcaagtcg gcgtttgggc caatccgatg aatacggttg aagagctgta tttggccaaa
     6781 aagcttgccg acggcttggg tgttaaaaac tttgccaccc gtctgcgcca acaagacaaa
     6841 cgtctttcag acggcattaa aggtgcgcaa tggttgggac aaagcattga atcgctggcg
     6901 gacaacgatg ccgtattggt agtcggtgcg aacctgcgca aagaacagcc gcttctgacc
     6961 gcccgcctgc gccgcgccgc taaagaccgc atggctttga gcgtattggc aagcagtaaa
     7021 gaagaattgt ttatgccgct tctgtctcaa gaagccgtac atcccgacga gtgggcaggt
     7081 cgtctgaaaa acctgtctgc cgatgcggaa cacgccgtta ccgccagcct gaaaaacgct
     7141 gaaaaagcag cggtgatttt gggcgcggaa gtgcaaaacc atcctgatta cgccgccatt
     7201 tatgccgccg cgcaagagct ggcggacgcg accggcgcag tgctgggtat tttgccgcaa
     7261 gccgccaaca gtgttggtgc ggatgtcttg aatgtaaact ccggcgagag cgttgccgaa
     7321 atggcaaacg cgccgaaaca ggcagtcttg ttgctcaacg tcgagccgga aattgatacg
     7381 gttgacggtg caaaagccgt agccgcgttg aaacaggcaa aaagcgtgat ggcgtttacg
     7441 ccgtttgtca gcgaaacgct gctggacgtg tgcgacgtgt tgttgccgat tgcaccgttt
     7501 accgaaacct caggcagctt catcaatatg gaaggccgtc tgcaatcttt ccacggcgtg
     7561 gtacaaggct tcggcgattc gcgtccgatg tggaaagtat tgcgcgtatt gggcaatctg
     7621 tttgacctga aaggttttga atatcacgat accgctgcga ttctgaaaga cgcgctggat
     7681 gcagaaagcc tgccgtccaa actggacaac cgtagcacat ggacagggga gggcgttcag
     7741 acggcctcag accgccttgt ccgtgtcggc ggcgtcggta tttatcacac tgattctatc
     7801 gtgcgccgtt ccgcaccgtt gcaagaaacc agccatgccg ccgtgcctgc cgcgcgtgta
     7861 aatccgaaca ctttggcacg cttgggcctg caagacggac aaattgctgt cgccaaacaa
     7921 aacggcgcaa gcgtatcggt tgccgtcaaa gccgatgccg gtttgcctga aaacgtggtg
     7981 catctgccgc tgcataccga aaatgccgcg ctgggtgcgt tgatggacac tattgaactg
     8041 gcgggagctt gatcatgcaa gaatggttcc aaaacctctt tgccgcaacg ctcggtctgg
     8101 gcgatttggg tattaccgta ggcttggtgg tatccgtcat cgtcaaaatt gtgattatcc
     8161 tgattccgct gattctgacc gttgcctacc tgacgtattt cgaacgtaaa gtcatcggct
     8221 ttatgcagct tcgtgtcggc ccgaacgtaa ccggcccgtg gggtctgatt cagccgtttg
     8281 ccgacgtgtt caaactcttg tttaaagaag taacccgtcc gaagttgtca aacaaagccc
     8341 tgttctacat cggcccgatt atgtcgctcg ccccgtcttt tgcggcatgg gcagtgattc
     8401 cgttcaacga agaatgggtg ctgaccaata tcaatatcgg ccttttgtac atcctgatga
     8461 ttacctcgct gtcggtttac ggcgtgatca tcgcgggctg ggcttccaac tccaaatatt
     8521 cgttcctggg cgcaatgcgt gcctccgcgc aaagcatttc ttacgaaatc gccatgagtg
     8581 ccgcgctggt gtgcgtcgtg atggtatcgg gcagtatgaa cttctccgac atcgttgccg
     8641 cgcaagccaa aggtattgcc ggtggttcgg tattctcgtg gaactggctg ccgctcttcc
     8701 ccatcttcat cgtttatctg atttccgccg ttgccgaaac caaccgtgcg ccgtttgacg
     8761 tggcagaggg tgagtctgaa atcgttgccg gtcaccacgt cgaatattcc ggcttcgcgt
     8821 tcgcgctgtt cttccttgcc gaatacattt tcatgattct gattgccgcg ctgacatcgt
     8881 tgatgttcct cggtggctgg ttgtctccgt tcccgcaaag ctggggcttt atcggtacgc
     8941 cttccgcatt ctggatgttc gtgaaaatgg cggcggttct gtactggtac ctgtggatcc
     9001 gtgcaacctt cccgcgctac cgttacgacc aaatcatgcg tttgggctgg aaagtgctga
     9061 ttccgatcgg cttcgcctac atcgtggttt tgggcgtgtg gatgatttca ccgctgaatt
     9121 tgtggaaata agtttcagac ggcatcttga ggccgtctga acaaagcgat tttgaatacc
     9181 taacgaaatc cctgttttga gggaacataa tatggctaac ttagtaaaaa cctttctgct
     9241 tggcgaattg gtaaaaggta tgggcgtaac gctcaaaaac tttttcgccc gcaaagacac
     9301 gatttatttt cccgaagaga aaacgccgca atccgtgcgt ttccgcggcc tgcacgcgca
     9361 gcggcggtat ccgaacggtg aggaacgctg cattgcgtgt aagttgtgcg aggcggtgtg
     9421 tccggcaatg gcgatcaata tcgaatcgga agaacgcgaa gacggcaccc gccgcaccaa
     9481 gcgttacgac atcgacctga ccaagtgcat cttctgcggc ttctgcgaag aggcctgccc
     9541 aaccgatgcg attgtggaaa cgcatatttt tgaataccac ggcgagaaaa aaggcgactt
     9601 gcacatgacc aagccgatcc tcttggcaat cggcgacaaa tacgaagccg aaatcgccaa
     9661 acgcaaagcc gctgacgcgc cgtaccgtta aggagcagac gaatgacttt ttccgcgatt
     9721 ctgttctata tccttgccac catcgtctta tacggcgcgc tgcgtaccgt taccgctaaa
     9781 aaccctgtcc acgccgcttt gcatctggtg ctgaccttct gcgtgagcgc gatgctttgg
     9841 atgctgatgc aggctgagtt tttgggcgtg acgctggtgg tggtttacgt cggcgccgtg
     9901 atggtgttgt tcctgttcgt cgtgatgatg ttgaacatcg acattgaaga aatgcgtgcc
     9961 ggtttctggc ggcacgcgcc tgttgccggt gtggtcggca cattgttggc ggttgcgctg
    10021 atcctgattc tggtcaaccc gaaaaccgac cttgccgcat ttggtctgat gaaagacatt
    10081 cctgccgatt acaacaatat ccgcgatttg ggcagccgta tttataccga ctatctgttg
    10141 ccgtttgaat tggcggcggt attgctgttg ttgggtatgg tggcggcgat tgcgctggtt
    10201 caccgtaaaa ccatcaatcc gaaacgcatg gatcctgccg accaggttaa agtacgcgcc
    10261 gaccaaggcc gtatgcgtct ggtgaaaatg gaagcggtca aaccgcaagt cgaatctgcc
    10321 gaagaaagcg aagtttcaga cggtctcaag ccggaagggg agggcaaagc atgattacct
    10381 tgacgcatta tctggtattg ggcgcgctcc tgttcggtat cagcgcgatg ggcatcttta
    10441 tgaaccgcaa aaacgtgctg gtcttgctga tgtccatcga gctgatgctt ttggcggtga
    10501 acttcaactt tatcgccttc tcgcaacatt tgggcgatac cgccggacag attttcgtat
    10561 tcttcgtact gaccgttgcc gctgccgaat ctgccatcgg tttggcgatt atggtattgg
    10621 tgtaccgcaa ccgacaaaca atcaacgttg ccgatttgaa caaacttaaa ggttgatttg
    10681 gaatttttaa agaaaaaagg agatagataa tgtttgatat tgctgctgtt ggattaggtg
    10741 cagcaggagt tagcttaatc aaacagttac aggtaaaaac tcttgtttgc catatcaagg
    10801 cactttggtc tccacacgcc catatatgca taaattgtgg attagtatga gtgaaagaga
    10861 atgtttacaa tttcaagggc tcattggcag ccattgggct gtatggcggg taattgggta
    10921 aaacaatggg tttttgaaaa ttttagaatt tcagaaagtg gagagtgatt atgtcatttt
    10981 caattgctct tccattggta tatctgttta ttggcatttt gattgggaaa acccctttta
    11041 gaaaaattaa aggcactttt tctgtaatat tgagcagatg gattatccct tttgttattg
    11101 tctataacat aacgacatat cgtgatggta tgcagtatgt tatgttatgg atgattattt
    11161 cgatggggtt gattatccta atcgggaaaa gagttgataa agatcctgtt tggaatttgt
    11221 gtttgtgcta tttgaacatc ggatggttgg ggcttcccat agttgcaacc ttgtttggag
    11281 atgaggcggc gcaagtgctt atttcggcat acgtgggaag ttctttattc ggaaattcgg
    11341 tcggggtcgg gttgttattg aataatagta atttcatatc cggattgaaa aagactttaa
    11401 aatcccctcc cgttgttgca ttggtaatag gaatgttcgg tattccgcta gggcattttg
    11461 tagcagatta ttttggggaa gtttattttt tctcgaaaat agcaatggga atattgggga
    11521 tggtgatact cggtatttgg ctggctgaga taaaactaaa tttgaatgat ttgaagaaaa
    11581 gtattaaacc gtttctaata aaaaacattt tatttatagg gattgttttt ctttttatcc
    11641 aaatagctgc atattttgac ttgaagttga ttcttgaaaa ccaaaaaaca ttgtatttaa
    11701 taccgttgct gccgcctgct gccaatatta ttgtgttgga aacggcatat ttgaaaacag
    11761 ggcgttcggc ttctatgatt gcgtatggta cgatttttag tttaatcgca atcagcattt
    11821 atattttact ggtgcaaatt taaatgaacg acatgacttt atatctcatc attgcccttg
    11881 tgcctttggc aggctcactg atcgcgggtt tgttcggcaa caaaatcgga cgtgccggcg
    11941 cgcatacggt tacgatactc ggtgtggcgg tgtctgccgt gttgtcggct tatgtgctgt
    12001 ggggctttat cgacggcagc cgcgccaagt ttgacgaaaa cgtctatacc tggctgacaa
    12061 tgggcggctt ggatttttcc gtcggcttct tggtcgatac gatgacggcg atgatgatgg
    12121 tcgtggtaac gggcgtgtcg ttgatggtgc atatctacac catcggctat atgcacgatg
    12181 aaaaagtcgg ctaccaacgc ttcttcagct atatttcttt gtttactttc agtatgttga
    12241 tgctgattat gagcaacaac ttcatccagc tcttcttcgg ctgggaggct gtgggcttgg
    12301 tatcgtatct cttgatcggt ttctatttca aacgcccgag cgcaacattt gccaacctga
    12361 aagccttttt gatcaaccgt gtcggtgact tcggcttttt gctcggtatc ggcttggtgc
    12421 ttgcctattt cggcggcagc ctgcgctatc aagatgtatt cgcttatctg cccaacgtcc
    12481 aaaatgccac catccagctt ttccccggtg tggaatggtc tttgattacc gtaacttgtt
    12541 tacttctgtt tgtcggtgcg atgggtaaat cggcacaatt cccgctgcat gtctggctgc
    12601 ccgattcgat ggaaggcccg accccgattt ccgcattgat tcacgccgca accatggtta
    12661 ccgccggtct gttcatggta tcgcgtatgt cgccgattta tgagatgagt agcactgcgc
    12721 tgtctgtcat catggtgatt ggtgcgatta ccgccctgtt tatgggtttc ttgggcgtga
    12781 ttcaaaacga catcaaacgt gtggttgcgt attccaccct gtcgcaactg ggctatatga
    12841 ccgtggctct gggcgcgtct gcctattccg tggcgatgtt ccatgtgatg acccacgctt
    12901 tctttaaagc cttgctgttc ttggcggcgg gcagcgcgat tatcggtatg caccacgacc
    12961 aagatatgcg ccacatgggt aacctgaaaa aatacatgcc gattacttgg ctgactatgc
    13021 tgatcggcaa tttgtcgctg atcggtacgc cgttcttctc cggcttctac tccaaagatt
    13081 cgattatcga agcggcgaaa tacagcaccc tgccgggcag cggctttgcc tatttcgccg
    13141 tcctcgccag cgtgtttgtt actgcgttct acgcattccg ccaatacttt atggtgttcc
    13201 acggcgaaga gaaatggcgc agcttgcctg aacatcatga cgatcatcat ggcgaagaac
    13261 atcacagctt gggcaaaaac gacaatccgc acgaaagccc gttggttgtt accctgcctt
    13321 tgattttgct tgccattccg tccgtcatca tcggctacgt tgccatcgag cctatgcttt
    13381 acggcgattt ctttaaagac gtgattttcg tcaataccga cgcgcatccg accatgcaca
    13441 tcatgaagga agagttccac ggcgcattgg caatggtgtc gcacagcttg acatcgcctg
    13501 ttttgtattt ggcggctgcc ggtgttgccg ccgcgtggct tttgtacgtc aaactgccgc
    13561 acctgcccgc caaaatcgcg caggcgttcc gtccggttta cgttttgttt gaaaacaaat
    13621 actacctcga cgccctgtat ttcaacgtct tcgctaaagg cacgcgcgca ctgggtacct
    13681 tcttctggaa agtcggcgat accgccatta tcgataacgg catcgtcaac ggctctgcca
    13741 aactggtcgg cgcgattgcc gcgcaagtgc gtaaagccca aaccggcttt atctacacct
    13801 acgccgccgc tatggtattc ggcgtattgg tactgctcgg catgaccttc tggggcttgt
    13861 tcagataaag gcggagtttc agacgacctg ttcatagttt tgtagaggtc gtctgaaaag
    13921 tttgagaagt gggcattaag tcaaagggtg gaacaaaatg gtacccaaca ggcgtgaaat
    13981 agccaacatg caaattatct gtttcctaaa ttttttaggc aatctcaaca aatatagagg
    14041 agtcaaaagg gatgacacag ttatttttac attcagatac accaaatgag aaggaactga
    14101 tttcaatata tgagaaagct gtttcagagg cagtagagct gtatattttc tctgcgtatc
    14161 tcactcattg ggatgtaaaa aataagctca atagcaaatt gcaagagttt aaatttattt
    14221 ttggacaaga tttcggattg tctaaaaaag aggctattcg gaaagtgttg aaatggcttc
    14281 cgtcccattt gaaattcaca ctgatggtag ctcaaggcat tcagggtttt catcctaaag
    14341 ctgtattttg gaagaatgat aagaatgaat attatgcttt aatcggttct tcaaatttga
    14401 ctcatgctgc gtttaatagc aattatgaag caaatatttt gactaaaatt tccgagcagg
    14461 attttataaa agtcaaatct tgggctgatg aaattgcgat gaaatctatc ccagtatcgg
    14521 aagattggct tgaggagtat caggaagctg aaattaatta taagaaatca actgttagcc
    14581 aatcattgat ggataagtta tttatggaaa tgccaaatta taatcaagaa ctgattgcag
    14641 cacggagaaa acagatgaga aaccatcaaa ctgtgtgtaa tcaattaaaa agtcttatta
    14701 agcaatgtgc tgcaggaaag atagacaatg atgagtttta tgtggaattt aataaattat
    14761 ggtcttggaa aagcgaaaat aaaggagaag gggtaggtaa tcgatttcaa gatcaaacgt
    14821 ggaaaaggac cggaaagtct tcagatttta gaaaattatg cattgctatc caatcagttt
    14881 ttgatgcacc acttgctgag agagataatg ttgttgccaa gcaaattgat tggctaaaag
    14941 aatgtggagt ttccactcgt ggttcagttt tttctgaaat gctttgtcaa gaatatccag
    15001 atagatatcc agtgttaaac gctcctatta aaaaattttt agaaaaaaat aaatttaagt
    15061 ctgcaaaagg agcaagtgaa ggctctagat atattgattt aagtatgaag ttaagagctt
    15121 tactagcaat gcagtcagaa ataaaaaatt tggctgaatt agatgtttta gtgcaggcag
    15181 aatatcgcga tagtacagat attgattggg agtgagtaaa gaggggagag agtattcatc
    15241 cctttttcag acgacctcct gaaacgtcgt ctgacaaacc aacccgtaca acgatatttt
    15301 tattttaacc acaggttaac cactatgttt tccaactacc tactcagctt ggcaatatgg
    15361 atacccatcg ccgcaggtgt gctggttttg tcgaccggta aggacagccg tgcgccgctg
    15421 gcgcgtgtgc ttgcctttat gggtgcgctt gccggtttct tggtaacgct gcccctgttt
    15481 accggtttcg accgtttgag cggcggctat caatttaccg agttccacga gtggattccg
    15541 ctgctgaaaa tcaactatgc attgggcgtg gacggtattt cagtgctctt tatcatcttg
    15601 aatgcgttta ttacgctgtt ggtggtgctg gcgggttggg aagttattca gaaacgcccg
    15661 gcgcagtata tggcggcatt cctgatgatg tcgggtttga ttaacggtgc gtttgccgcg
    15721 caggatgcga ttctgtttta tgtgttcttc gagggtatgc tgattccgct gtacctgatt
    15781 atcggtgtat ggggcggccc gcgccgcgtc tatgcgtcgg tcaagctgtt cctctatacg
    15841 ctgatgggtt cgctcctgat gctggttgct atggtttacc tgtactatca aacaggcagc
    15901 ttctctattg tcgatttcca aaacatcaaa cagattccgt tgggcgtaca acagctttta
    15961 tttgcagcgt tcttcctgtc atttgccgta aaagtgccga tgttccctgt gcacacttgg
    16021 ttgccggatg cccacgttga agcgccgacc ggcggttcga tggtgttggc ggcgattacg
    16081 ctgaagctgg gtgcgtatgg tttcttgcgc tttatcctgc cgattatgcc ggatgcggca
    16141 cgctattttg cccctgtgat cattgtattg agcctgattg ccgtgattta catcggtatg
    16201 gtggctttgg tgcaaaccga tatgaaaaaa ctggtggcgt attcgtccat cagccatatg
    16261 ggttttgtca ctttgggtat gttcctgttt gtgaacggcc aattgaatga ttgggcattg
    16321 aaaggcgcaa tcattcaaat gatttcccac ggttttgtgt ctgccgcgat gtttatgtgt
    16381 atcggcgtga tgtacgaccg cctgcatacc cgcaatattg ccgattatgg cggcgtggtc
    16441 aatgtgatgc ctaagtttgc ggcgtttatg atgttgttcg gtatggccaa tgccggtctg
    16501 ccggcgactt ctggcttcgt gggcgagttt atggtgatta tgggcgcggt taaagtgaat
    16561 ttctgggtgg gcgcgttggc ggctatgacg ctgatttacg gtgcgtctta taccctgtgg
    16621 atgtacaagc gcgtgatttt cggcgcgatt cacaatccgc acgttgccga aatgaaagac
    16681 atcaactgtc gcgagtttac gattctggcg gttttggcgg ttgcggtatt gggtatgggc
    16741 ctgtatccga acgcatttat cgaagtggtg catcaggcgg caaacgattt gattgcccat
    16801 gtggcacaaa gcaagatttg aggtgtgtaa atgaactggt ctgatttgaa tttaatgccc
    16861 gccatgcccg aaatcgtgct gcttgcgctg ctggtgttat tgttgctggc ggacttgtgg
    16921 gtcagtgatg acaaacgccg gtggacgcat tacggcgcgt tggcaacggt ggcggttacg
    16981 gctgtggtgc agttggcggt gtgggaacag ggcagcacgt cttcgttcaa cgggatgtat
    17041 attgcagacg gtatgtcgcg tttggcaaaa atggttttat atgccttgat gtttaccctg
    17101 tttgtctatg ccaagcccta caaccaagtg cgcggtattt tcaaaggcga gttttacacc
    17161 ctgtcgctgt ttgccctctt gggtatgggc gtgatggtga gcgcggggca ttttttaact
    17221 gcctatatcg gtttggaact cttgtcgctt gccctttacg ccctgattgc cctgcgccgc
    17281 gattccggct ttgccgccga agccgccttg aaatattttg ttctgggtgc gctggcatcc
    17341 ggcctgctgc tctacggtat ttctatggtt tacggcgcaa ccggttcgct ggaatttgcc
    17401 ggcgtgctcg cctcttcctt caatgaagaa gcaaacgaat ggctgttgaa actgggtctg
    17461 gtgtttatcg tcgtcgccgt tgcgttcaaa ctcggcgcgg tgccgttcca tatgtgggtg
    17521 cccgacgtgt atcacggcgc gcccacttct gttaccgccc tggtcggcac tgccccgaaa
    17581 atcgccgccg ttgttttcgc tttccgcatc ctcgttaccg ggttgggaac cgtgcatcat
    17641 gactggtctc tgatgtttgc cctgcttgcc gccgcctcgc tgctggtcgg caaccttgcc
    17701 gccatcatgc agaccaatat caaacgtatg ctcgcctatt ccaccgtatc gcatatgggg
    17761 tttatcctgc tggcgtttat ggcgggcgcg gtcggctttg cggcgggtct ctattacgcc
    17821 attacctacg cgctgatggc ggcggcaggg ttcggtgtgt tgatggtgtt gtcggacggg
    17881 gacaacgagt gcgaaaacat cagcgatttg gcagggttga accaacaccg cgtatggctt
    17941 gcctttttga tgctgctggt tatgttctct atggcgggca ttccgccgct gatgggcttt
    18001 tacgccaaat tcggcgtgat tatggcactc ttaaaacaag gctatgtttg gttgtccgta
    18061 tttgccgtcg tgatgtcgct gattggcgcg ttctactacc tgcgcgtggt caaagtcatg
    18121 tattttgacg aatccggccg cgcccgtccc gccgccggcg gcaacaatgc cgcaaaattc
    18181 ctcttaagca tcaacgcgct gctgttggtg ctgtggggca ttatgccgca aaccgttatc
    18241 gactggtgcg ccaaggcgtt ggagaacacg ctgtaagccg ccgcaacggc agccgtgtca
    18301 gaggctgccg tttttgttaa gatatgccgt tccgcaatgc agttcagacg gcatcgccgc
    18361 cgacaatgcc caaacagaaa gcccaccatg accgcatcca tgtacatcct tttggtcttg
    18421 gcactcatct ttgccaacgc ccccttcctc accaccagac tgttcggcgt ggccgcgctc
    18481 aagcgcaaac atttcggaca ccacatgatc gagctggcgg caggtttcgc actgaccgcc
    18541 attcttgcct atattctcga atcccgtgca ggatcggtac acgatcaggg ttgggagttt
    18601 tatgccacag tcgtctgcct gtacctgatt tttgcgtttc catgttttgt gtggcggtat
    18661 ttttggcaca cgcgcaacag ggaatagaca agcatagaaa tgccgtctga aacctgttca
    18721 gacggcattt gtttcattca agtgcaggcc ggcatcgctg tgccggcacg tttcagccgg
    18781 cgatatacgc cggttttaat atttgcgggc gactgcaaat tctgccaact gccgcaggcg
    18841 cagggctttg tcgccgaagg gttcgagcag cgcgaccgct tcggcaacca gtttgtgtgc
    18901 gtatgagcgc gccgcttcca agcccatcag tttcacataa gtcggcttgt cgttgtctgc
    18961 atctttgccc gccgtcttgc ccaaagtcgc cgtgtccgct tcacaatcca acacatcgtc
    19021 aatgacttgg aacgccagcc ccagttttgc cgcataatcg tccaataccg ccagactgtc
    19081 ttcagacaga ttcgggcagg acatcgcccc caataaaacc gccgcacgga ttagcgcacc
    19141 cgttttcagg ctgtgcatct gttccaaatc ggcttgaacc atttgtttgc cgacattcgc
    19201 caaatcgatt gcctgaccgc ccgccatgcc cgcgctgccg cccgctttcg ccaacaccga
    19261 caacattgcc aactggcgtg ctgcgggcag ttctgtcgga cggcttaaca cgtcgaaggc
    19321 ttgggtttgc aaagcgtcgc cgaccagtag cgcggtggct tcgccatatt tgatgtggca
    19381 ggtcggtttg ccccgacgca ggctgtcgtt gtccattgcc ggcatatcgt cgtgaaccaa
    19441 cgaataggca tggatcattt cgattgccgc cattgcctgt tctactgctt catgcacggc
    19501 ttcgcctaat tccgaagctg ccagaaccag catcggccgc agacgtttgc ccccatccaa
    19561 aaccgcataa cgcatggctt cgtgcagtgt gtgcggtatt tcgttttcag acggcaaaaa
    19621 gcgttccagc agcagctctg tttgcgcctg cgccctctgt tgccacgctt tcaaatcatt
    19681 cgtcggattc aaggttcaac tccttcggcc cgtccgcgtc caaaacctgt aatttctgtt
    19741 cgacttgcgc cagcttggtt tggcagtatc tgaccagctc gttgccttcc tgataggcgg
    19801 caagcgcgtc ttccaagggc atttcgccct gcatagcttg tgtcagcgat tcaaggcgcg
    19861 acaaggcttc ttcaaacgat ttcggggcgt ttttcttcat cgtgtttcct tttcggttga
    19921 aaccccgcca cttggacatc tgtccttcgg ggcggtagaa tcagacttta tttgggaggg
    19981 gtgtaagccc ttccaaatca ggacgacaca tagggcggtg ctttaagtgt cgtcctgcgt
    20041 gttggaacat agtttcggat gttccagtaa aaagcggatt gtagcatttt tgaaaaacgg
    20101 atgccgtctg aaacccgaat ccggcttcag acggcatttt tccgcccagg cggcaaggcg
    20161 ttacccgggc agttcgtcgg tgatgccctg caaaaaggcg aggcgttcgg ggcttgccgc
    20221 cccggtttgc gcggcggctt tgaaggcgca gccgggttcg gcgcggtggg tgcagttgtg
    20281 gaagcggcat tgcccgacaa ggtggcggaa atcggggaaa tagcgcggca aatcggcggc
    20341 ttggaggtgg tgtaaaccaa attcttgcaa acccggggag tcgatgagtt gggtttcgcc
    20401 gttcaaatca taaagccggg cgtgggtggt ggtgtgtttt cccgagtcga gtgcggcgga
    20461 aatgtcgccg gtgcgggcgg tttggctgcc caaaagggcg ttggtcaggg tggatttgcc
    20521 cataccgctc tgcccgagca ggatgttgct gtgcccttgc agggcggggc gcaggctgcc
    20581 ggcgttttcc agtgcgcggg tttcgatgac gggataaccc agcgtttcgt agaatttgag
    20641 tttttcgcgc caaagggcgg tttcgggcag gtcggctttg ttcaggacga tgacggcgcg
    20701 gatgccggcg gcttcggcgg caagcagggc gcgttgtagc agccgcatgc tcggactcgg
    20761 gacggcggcg gttacgatga ggagttgggt aacgttggcg gcgatgagtt tggttttcca
    20821 cgcgtcttgg cggtagagca ggctttggcg cggtaaaaaa tcttcaatca caacttgttc
    20881 ggcgttgacg gggctgatgc ggacgcggtc gccgcaggcg aaatcgacgc gttttttgcg
    20941 ggtgctggct tcgtaggttg tgccgtcggg cgtgcggaca atgtagcggc ggccgtagct
    21001 ggcggtaatt tgggcggtgt cgttcatggt ttctttgggg ttgggtgtgg aaatgccgtc
    21061 tgaaaacggg tgttcggacg gcatcggttc agtcgtgctg ccactcgacg tgttcgttga
    21121 ggaagccgcc gctctggtgc gcccagagtt tggcgtaaag cccgcgtttt tcgaggagtt
    21181 cggcgtgtgt gccttcttcg atgatgcggc ctttgtcgag gacgacgagc ctgtccattg
    21241 cggcgatggt ggagaggcgg tgggcgatgg cgatgacggt tttgccgtcc atcattttgt
    21301 cgaggctttc ctggatggcg gcttcgactt cggaatcgag cgcgctggtg gcttcgtcga
    21361 gcagcaggat gggcgcgtct ttgagcatca cgcgggcgat ggcgatgcgc tggcgttgcc
    21421 cgccggagag tttcacgccg cgttcgccga cgtgcgcgtc gtagccgcgc cgccctttgg
    21481 catcggaaag gtcggggatg aagccggcgg cttcggcgcg ttcggcggca gaaaccattt
    21541 cggcatcggt cgcgtcgggg cggccgtaaa taatgttgtc gcgcacggaa cggtgcagca
    21601 gcgaggtatc ttgcgtgacc aaaccgattt gggcgcgtaa agattcttgg gtaacgccgc
    21661 ttatgtcctg cccgtcaatc aaaaccgtgc cgctttgcgg ttcgtagaag cgcaaaagca
    21721 ggttgacgat ggtggatttg cccgcgccgc tgcgtccgat caagccgact ttttcgcccg
    21781 ggcggatggt gaggttgaag ccgttgagca gcggtttgcc cgcttcgtag gagaaatcga
    21841 cgtgttcgaa cttgatttcg ccttgcgaca ctttcagcgg cagggcgttc ggcttgtcga
    21901 ggatggtgtg cggtttggac agggttgcca tgccgtcgcc gacggtgccg atgttttcaa
    21961 acagccgcgc ggattcccac ataatgtatt gcgacaaacc gttgacgcgc aacgccatgg
    22021 cggtggctgt agcaaccgcg cccacgccga cctgcccgtt gtgccagagc cagatgccca
    22081 gtgcggcggt ggagagggtc agggaggtgt tgacgatgaa gctgcacgaa tgcagcagcg
    22141 tcgccagccg catttgggcg cgcaccgtaa ccataaattc ttccatcgac tgcttggcat
    22201 aggcggcttc acgcgcgccg tgggagaaga gtttgacggt ggcgatattg gaataggcat
    22261 cggtaatgcg gccggtcatc agcgagcggg catccgcctg ccatgcggcg gtttgcccca
    22321 atttgggaat cagcaggcgc atcaccgaag cgaaaccgac aatccagccg ataaagggca
    22381 gcagcagcca tgagtcgagc gaggcgagaa tcacgccgga ggtaatgaaa tacaccgaca
    22441 cataaacgac catatcggca accgtcatca ccgcgtcgcg caacgccagc gcggtctgca
    22501 tgactttggc ggacacgcgt ccggcaaatt cgtcctgata aaaaccgagg ctttggttca
    22561 gcatcaggcg gtggaagttc cagcgcaggc gcatggggaa cacgccctga agggtttgca
    22621 ggcgcacgtt ggacgcggca aacgcccacg caaccgaaaa caccatcatc gccgccattg
    22681 ccgccagttc ccaacctttt tcggcaaaca gttcggcggg cgcgtatttg ccgagccact
    22741 ccacgatttt gcccataaat tgaaaaacca gggcttccat aatgccgatg ccggcggtca
    22801 gcgcagccag ggcggctatc catttccgca cgccggccat gctgctccag acaaaccgcc
    22861 acaagccttt ttctggcgtt ttcggggcgg cttcgggata agggtcgatt cgggactcga
    22921 accaggaaaa tattttgttc aacattgttt tcgatttcgg taaaacagtt tcagacggca
    22981 tcaaacacaa tgccgtctga aaggaaggac aataacgcca ttttacggga aaagccgtcg
    23041 ggaagacagc gcgaggcgga aacgcagggt ttcgtcaggg caaacgtcgc gccgccttca
    23101 gacggcatta tttcagcagg tttttcaaag caaggcgcac gccttcgccc acatccgtcc
    23161 ctttcggaac gcctttgacc gccgcttttg cttcgcgttc gctgtaaccc agtgcaagca
    23221 gcgtgctgac gatgtcttcc gtttcgtcgg cggcgggtgc ggcggcaaac agcccgtccg
    23281 ttaccgtatg cgcgaccagc ttgccgcgca gttccaaaac catacgttcg gcggtttttt
    23341 tgccgattcc cggggcggag gagaggcgtt tgacatcttc ttctgcaacc gcctgcgcca
    23401 gttcgtcggc agtcattgcc gacaaaatgc ccaaagccgt tttcgcgccg atgccgccga
    23461 ccttaatcag ttggcggaag gtcttgcgtt cttccgcagt ggcaaaacca aataaaagat
    23521 gtgcgtcttc ccgaatgata agctgggtaa acagttgtac gctttcaccc acgggcggca
    23581 ggttgtagaa ggtctgcatc gatacgtcgg cctcatagcc gacaccgttg acatcgatga
    23641 cgatttgcgg agggtttttt tcaaccagtt tgccggtcag tctgctgatc atgtatgccg
    23701 aatcctgaag tgtcgggtgc aaaatgccgt ctgaaaccgg ttcgggcttc agacggcacg
    23761 gattgtatca aattcagtcg tcgcggcggg aggaaatcac gcggccggta cgggcatcga
    23821 caacgacttt gtattcctgt ccgtttttga cgatttcgac atcatagtgc ggacggccgt
    23881 tgtcgtgttc gagatcgatg tcggtgattt tgccgccgac acgcgccaac gctgcttttt
    23941 cggcttgggc gcggctgatg attttgtctt gtttgctgtg ttggtgtgcg gcgtgtccgt
    24001 ggtcgtcatc gccgtgtccg tcgtggtggg cgagtgcggg ggcggaaatg cccatcagtg
    24061 cggttgcggc ggcggtcaag agaaggcgtt tgaagttcat gttttgctcc tgcgaagtat
    24121 ctggtggtgt ttgaaagacg taaaggcggg acatcaaccg gcagtcaata ccgcccgaac
    24181 cataccgcgc gcctgagctt cggcttcggc ggcgcgttcc tgcgaggcaa acggtcccat
    24241 tttgacgacg tattcgtaac ggcgtttttc aaccgagagg ttcgtacccg atgacgaaac
    24301 ggcgaagttt tgggcggctt ggttcagata ggcttgtgct tcgtgttccg taccgaaaga
    24361 tttcaagtcg ataaagatgt ctttgttttc ggcaaccggt gcggatttgc ccgggacgat
    24421 ttgttcgatt ttgacgtgtg ccgtcccttg gttgacaaag cccaattttt gcgcggcggc
    24481 tttggatacg tcgatgatgc ggttgccgtg gaaggggccg cggtcgttga cgcggacgat
    24541 gacgcttttg ccgtttttgg tattggttac gcgcacatag ctggggatgg gcagggtttt
    24601 gtgggcggcg gtaaaggcgt tcatatcgta tcgttctccg ccggaagttt tgcgcccgtg
    24661 aaacctgccg ccgtaccacg aggcgttgcc ggtttgcgtg aattcggcga cttggttttt
    24721 cggcgtgtag cgttttccgg cgactttgta gctgcggttg gcggaggcgt gcagtttttc
    24781 tgccttgacc actgcgtcgg cggatgccgt ctgaagggag tgtgtgccga atgcggcggt
    24841 gagaaggaaa agggtttttc gggttaaagt caaaacgtgt tccgttcttg agttgaagac
    24901 gaatgggcat catgcccgcc ggatacgttc cgaaccgccg tacagtgcgg acggcggttc
    24961 ggaatgtgtc cggataggtt ttcagacggc atgaacttgc gttcaaacgc cgcctgcgta
    25021 accgtgttgc cgccacgctt caaagagaat cacggcaacg gtattggaaa ggttcatact
    25081 ccgactgccg ggctgcatgg gcaggcggat tttttgcgcg gcgggcaggc tgtcgaggat
    25141 gtcggcaggc agtccgcgcg tttccggccc gaacagtaaa acgtcgcctt tttgaaacgc
    25201 ggtttcatcg gggcgcgccg tgcctttggt ggtcagggcg aaaatgcgcc tgcctgcgag
    25261 tgccttgagg cagtcgtcga agttttcgta caccgtcagg ctggcgaatt cgtggtagtc
    25321 gagcccggcg cgtttcattt tggcggaatc caatgggaag ccgagcggtt tgacaaggtg
    25381 caaatccgcg ccggtattgg cgcacaggcg gatgatgttg cccgtgttcg gggggatttc
    25441 cggttggtat aaaacgatgg taaacataaa tatcaatcac ttataggcgc gtaaccttgc
    25501 cacaaggcgg atggggtgtc aaaaaattta gttatttttt cattggcgtg cgtgccagcg
    25561 tccagcagca gatgcggttt gcgcctaatt ttttcagcgt ctttgccaat tcgtccagcg
    25621 tcgcgccggt ggtaaagaca tcgtcgatta acagaatatt acagttttcc ggtatcggtg
    25681 tgcggatttc aaaggcgttt ttgatgtttc gccgccgttc gccgcctttg agtgtgcttt
    25741 gcggcgggcg gtggtgtcgg aaaacggtgt gtcggggcag tatctgccag ccgtagcgtt
    25801 gtgccagcag cccgacgatg ctttcacttt ggttgaaccc gcgttgcagc agccgctccc
    25861 tgcttagcgg tacgggcagg acgaaatcga aacattcgtc tgcaagccgg tcgggcggat
    25921 tctgcatcat caggtctgcc agcggctgca ccatgctcaa atcagccaag tgcttcagcg
    25981 cgtgtatcat attgctgacg ggcggttcgt aatgcagcga agcccacatc cggtcgaatg
    26041 cgggcggttt tttctgacag ccgccgcaca ccgcgccgcc ttggatgtgt ctgaaacaca
    26101 gggggcagct gtttgccgcg tcggtgcggt atgccgccaa atccgccgcg cagccggcgc
    26161 agatgccgtc tgaaacgccg gacgaaccgt ggcataatac gcaacgcctg atagtgggcg
    26221 cgtctgcgat gcgccgccaa caagagagaa aatccatgcc tgatgccgtc aaaaaagttt
    26281 acctgataca cggttggggg gcgaaccgcc acgtgttcga cgatttgatg ccgcgcctgc
    26341 ctgcaacgtg gccggtgtcc gccgtcgatt tgcccggaca cggcgacgcg cccttcgcgc
    26401 aacccttcga tattgaggca gccgccgacg ctgttgccgc acaaatcgat acgcccgccg
    26461 acattctcgg ctggtcgctc ggcggattgg tcgcgctgta tctggcggcg cgccatcccg
    26521 acaaagtccg ttcgctctgc ctgacggcga gtttcgcacg gctgacggct gccgaagatt
    26581 atcccgaagg gcttgccgcg cctgcattgg gcaaaatggt cggcgcgttc cgaacggatt
    26641 atgccaaaca tatcaaacag tttctacaac tccagctttt gcacacgcat gatgcggaca
    26701 gaatcatagg cagaatcctg cccgatttgg cgcgctgcgg cacgccctcc gccttacagg
    26761 aagctttgga cgcggcggaa agggcggatg cgcggcattt gttggacaag atagatgttc
    26821 cggtactgct ggtgttcggc ggcaaagacg cgattacacc gccgcgtatg ggtgagtatc
    26881 tgcatcggca tttgaaaggc agcaggctgg ttgtgatgga aaaggcggca cacgcgccgt
    26941 ttttgagcca tgcggaagcg tttgccgcgc tgtaccgcga ctttgtcgaa ggggtttgac
    27001 atgaatccgc aggacaaatg ttggcaggtt caccgccatc ttgccgaaca tgccgaccaa
    27061 cggctgacac tcgtccgcaa cgcgcccaaa catatcctgc ttgccggggc ggatgcggac
    27121 atcagccgca gcctgctggc gaaacgctat ccgcaggcgg tatttgaaga atacgattcc
    27181 cgtgcggatt ttttggcggc tgccgcagcc gtgcgcaagg gcggtttttg gcaaaggttt
    27241 acgggtaagg gcgtggtgca acactgccaa tccccgaccg cgccgctgcc cgaagcgtgt
    27301 gccgatatgt tgtggtcgaa tctcggactg ttggcggcgg aacaaatcct tcctgtgctg
    27361 cacaactggg cgcgcgcgct ggagacggac gggctgctgt ttttcacctg cttcgggcga
    27421 gataccttgg cggaactgaa atgccgtctg aaagaaaatg gcattgaaag ccgcagcgcg
    27481 cttttccccg atatgcacga cttgggcgat atgcttgctg aaaacgggtt ttacgacccc
    27541 gttaccgata cggcgaagct ggtgttggat tacaaaaagg cggaaacgtt ttgggcggat
    27601 atggacacgc tgggcgtttg gcgggcgatg tcgtggaacg atgaaaacgc cgcgcgttcg
    27661 tgtgtcggga caatatttga acgggaaggc ggtttgagta ttacgctgga aacagtgtac
    27721 ggacacgccg ttaaaaaact gatgctgccg caaggggaga acgtggtgca gttttttccg
    27781 aagagatgat gtgcagatgc cgtctgaagc cgtttccaag tttcagacgg catttgtttg
    27841 tgaaaaccga cagaactaaa ggaaatgccg atgtatagtg aattaaattt aaaccagtac
    27901 agcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga
    27961 tttttgttaa tccactatat gctgatgccg gagacgtata ttgcgtctat aacatcagac
    28021 tgaagcagta cactgcctgc caggttaccc gagttgaaga acacggtggc aaaaaaacac
    28081 atgcgaccct gctggctttg gactggcagg gcaacaaacc gcttggggcg gaggagctgg
    28141 cggatttgaa accactttac aaagacttaa agaataatat tggaaatatt gtatgaacaa
    28201 aaaattaaac tatattttta tgttggactg tttagggttg gtgatattgt ttacttgtat
    28261 aatagctact tttgaaagag attatggatt taaaattttt actaattcta agaggcctga
    28321 attttattat tggattggaa tgttttatta tggaattatt tcttgctggt ttgattatca
    28381 attaatttca acaaaggcga attcgtataa aagaaaagtt aaacaatata aaattttttc
    28441 agtaatattt tcagttttga tatttatttc tactatagta aaactttaaa ttttggagca
    28501 aaaatttatg agcgattcaa ttgaatatgt attgggaacg cggtctgcac atgtataagg
    28561 caagtgctgt cgtgccaacg ggatatgtac gggttgggaa taccgcgccg ctggtcggcg
    28621 aagacacgca acggtatgcc tctttttggg gcgacggcta cgacgtgtac cgtcagttga
    28681 gatggcagca gatacccgaa aaacagagaa aggcattcaa aaaagccgcc aaaagcaaaa
    28741 agaccgtgat gtttgccgga cgggaatacg gcatatccaa acagaatttg agcgatgttt
    28801 gggatgattt tgaagacgcg atggaactga aggcgtttcc ctgcctgtct tcgctgtttc
    28861 tgaccaaatg gcataaaaat ctatatgata gtggattaac aaaaaccagt ac
//
LOCUS       DAWKQJ010000008        25901 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000008 DAWKQJ010000000
VERSION     DAWKQJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.008"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   375..1862
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=441..476
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(1926..2252)
                     /gene="cas2"
                     /locus_tag="WJL20_000272"
     CDS             complement(1926..2252)
                     /gene="cas2"
                     /locus_tag="WJL20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ7682473.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2245..3159)
                     /gene="cas1"
                     /locus_tag="WJL20_000273"
     CDS             complement(2245..3159)
                     /gene="cas1"
                     /locus_tag="WJL20_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ7682474.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAKHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(3226..6474)
                     /gene="cas9"
                     /locus_tag="WJL20_000274"
     CDS             complement(3226..6474)
                     /gene="cas9"
                     /locus_tag="WJL20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ7682475.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRACRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            complement(6752..10186)
                     /gene="dnaE"
                     /locus_tag="WJL20_000275"
     CDS             complement(6752..10186)
                     /gene="dnaE"
                     /locus_tag="WJL20_000275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ7682476.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLANGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAEPQIQELIEVEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQDNIKNTTGDIVDVGTIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10726..10959
                     /locus_tag="WJL20_000276"
     CDS             10726..10959
                     /locus_tag="WJL20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7682477.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            10937..11146
                     /locus_tag="WJL20_000277"
     CDS             10937..11146
                     /locus_tag="WJL20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682478.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSEDENAV"
     gene            11442..12278
                     /locus_tag="WJL20_000278"
     CDS             11442..12278
                     /locus_tag="WJL20_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ7682479.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDETDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIQAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGVGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCKPVWAHFS
                     RH"
     gene            complement(12329..13621)
                     /locus_tag="WJL20_000279"
     CDS             complement(12329..13621)
                     /locus_tag="WJL20_000279"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ7682480.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13679..15589)
                     /gene="pglD"
                     /locus_tag="WJL20_000280"
     CDS             complement(13679..15589)
                     /gene="pglD"
                     /locus_tag="WJL20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ7682481.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDHIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15637..16812)
                     /locus_tag="WJL20_000281"
     CDS             complement(15637..16812)
                     /locus_tag="WJL20_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ7682482.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGQGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVGELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLTNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            complement(16937..17131)
                     /locus_tag="WJL20_000282"
     CDS             complement(16937..17131)
                     /locus_tag="WJL20_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682483.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPRDLDSISD"
     gene            complement(17179..17865)
                     /locus_tag="WJL20_000283"
     CDS             complement(17179..17865)
                     /locus_tag="WJL20_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7682484.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
     gene            complement(17852..19429)
                     /locus_tag="WJL20_000284"
     CDS             complement(17852..19429)
                     /locus_tag="WJL20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ7682485.1"
                     /translation="MNQFFKRLFDIVASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMIKFRSMRDAIDKDGNPLPDSERLTDFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(19401..20590)
                     /gene="pglH"
                     /locus_tag="WJL20_000285"
                     /pseudo
     CDS             complement(19401..20590)
                     /gene="pglH"
                     /locus_tag="WJL20_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            complement(20610..21683)
                     /locus_tag="WJL20_000286"
     CDS             complement(20610..21683)
                     /locus_tag="WJL20_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7682486.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPPEFHFRKTKKECAVLGTLSRLNTVRAVHLMLDIFKKMVDRDMPVRLNVAGIG
                     EEMDNLKAQAKRLGIEDKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            complement(21680..23101)
                     /locus_tag="WJL20_000287"
     CDS             complement(21680..23101)
                     /locus_tag="WJL20_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ7682487.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYLHTLFCLTSSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(23296..24405)
                     /gene="ribD"
                     /locus_tag="WJL20_000288"
     CDS             complement(23296..24405)
                     /gene="ribD"
                     /locus_tag="WJL20_000288"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ7682488.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARKLNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGGGSGLFCLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(24437..24901)
                     /gene="nrdR"
                     /locus_tag="WJL20_000289"
     CDS             complement(24437..24901)
                     /gene="nrdR"
                     /locus_tag="WJL20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ7682489.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAIRFAALHKRFDSPEHFASWLAQAVKTGGKA"
     gene            complement(24930..25772)
                     /locus_tag="WJL20_000290"
     CDS             complement(24930..25772)
                     /locus_tag="WJL20_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ7682490.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVQHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         5432 a         6385 c         7565 g         6519 t
ORIGIN      
        1 tttgcaagat tttagtttaa tcatcaaaca gggaacagca aaaatccccg ctgcaacggg
       61 gatttttgtt cggctctcgc ctgtgtgatt aacttcacta ggagaagcta aaatgcttaa
      121 attccttatg cgtgggaaaa agttgctgct gtccgttgaa gtgaaccaca agttcatttt
      181 agcggttgct ttgctgttaa gtcaatgcag tagctaaccc gaccacaatc aaaaaaacgc
      241 aagttttccg cctactctgt gagtgggcgg ttttttctta tttcaaatct gtgacacaat
      301 atctgtcgcc tttcttgcca taattgcact gtgtctcaat gactttaacg cacgttcgct
      361 tatcgcaacg gctggttgta gcgcccattc tcatttcgca gtgctacaat gcggaacggg
      421 ttgacagact gaatttgagt gttgtagctc cctttctcat ttcgcagtgc tacaataggg
      481 gtcgtgaatg gagttcttgc agttcggttg tagctccctt tctcatttcg cagtgctaca
      541 attaagcgtt tcttttttgg tctgcttctg ctgttgtagc tccctttctc atttcgcagt
      601 gctacaataa tcagaccgac tttacaacga aggtttgagt tgtagctccc tttctcattt
      661 cgcagtgcta caattgctac cagcgacaac aggcattacg cccagttgta gctccctttc
      721 tcatttcgca gtgctacaat tgagaaagat ttaagaacta agttcccatc gttgtagctc
      781 cctttctcat ttcgcagtgc tacaattcag cctcaaatac atccgccaaa tcgttggttg
      841 tagctccctt tctcatttcg cagtgctaca atacacaatc tccgccgttc caatcaaatg
      901 tggttgtagc tccctttctc atttcgcagt gctacaatct gatgaagata caaccgcact
      961 aactttacgt tgtagctccc tttctcattt cgcagtgcta caataatgat gtatataaaa
     1021 tgccgtaaaa aatggttgta gctccctttc tcatttcgca gtgctacaat cgtctgaagg
     1081 tttgcccatc tcctgacagg gttgtagctc cctttctcat ttcgcagtgc tacaattcga
     1141 ttgtctatga aagcacttga tgatttgttg tagctccctt tctcatttcg cagtgctaca
     1201 atctcttcat tgaagaagtt ataagccttt gtgttgtagc tccctttctc atttcgcagt
     1261 gctacaattt tggtaaaggt ttctgttgcg acccgaatgt tgtagctccc tttctcattt
     1321 cgcagtgcta caatgcctgc gtacttggta cgctggtcgc ctccgttgta gctccctttc
     1381 tcatttcgca gtgctacaat ttcatagatc gatgcatgag gttttcatgg gttgtagctc
     1441 cctttctcat ttcgcagtgc tacaataggt caaagtgtca ggcaaattta aaggtggttg
     1501 tagctccctt tctcatttcg cagtgctaca ataaatccat ttatcgccca ccctttccgg
     1561 ccgttgtagc tccctttctc atttcgcagt gctacaattg ctgaaattat ggcaagtatg
     1621 gggcaaaggt tgtagctccc tttctcattt cgcagtgcta caattagcaa acaaggaata
     1681 ttcaaagtcc tttagttgta gctccctttc tcatttcgca gtgctacaat cggcaatgcg
     1741 gcggctgtgt tggttggcga gttgtagctc cctttctcat ttcgcagtgc tacaatcagc
     1801 tatatcgcgt tctgactttc tgcctggttg tagctccctt tctcatttcg cagtgctaca
     1861 atacttcgac gggaaatcct tatttcataa gggtttcccg ttttatttgt tatgaaaaaa
     1921 tgggcttaaa ataacaataa ttggtctgaa ttgacctttt tttcttgggt tttcagctcg
     1981 cccaacagca gtttcatggc ggcatattgc ttttctgtta tctccaaaca gcggattgag
     2041 ccttcttgcg gaaggtttgc acacagtctg ttgtggtgtt tttgcaacga atcgcggcct
     2101 ttgacgatac ggctgtatac ggaaagttgc agcatttggt atccgtcttt taataaaaac
     2161 tggcggaatt gattggcggc tttgcgcttt gccgccgtga taaccggtag gtcgaagaag
     2221 acgataatcc tcataaattt ggcctcactc atattggtat tctttcaatg gtaggatttc
     2281 aggcagtttc agttgtttgg cgtttttatc ggttacgccg gcttgaaatg acgaaatcat
     2341 tttgtcgatg gcagccaagg tgctgaaatg ttgccgttcg atgctggttt gatagtagag
     2401 gattttgatg aggtgttgtt tgatgccgag cgtcagttca gttttcagac ggccttgttc
     2461 gtacaaatgt atcacggtca ggtcggccaa tgggcgtaag ggttcgataa agtcatcggc
     2521 gagattaaat ggattgagtt cacttctatg aaacaagcct aatgcaggta accaaccgta
     2581 taaggtcagg gcgcgtgcaa ctgcggcacg gagtacggcg taggtgtagt tcaatgcggc
     2641 attgacggcg ttgttgtcat tacgggtaaa tttttcgcca aagagggctt gaaagtagag
     2701 ggcggcagct tgggcttcac ggtttcctgt atcgcctgaa cggacttcag atgccaaagt
     2761 gcgcagccgt ttggctgcaa gatcattgcc tgtttcgtct gcgacaaatg cttggttcag
     2821 gattttttgg cggacgatat gttgccagag ttgctttttt aagggttcgc ttatgttcag
     2881 ttgcagtttg aggattttaa gttggcggtg atattgggca taaggcagcc attgtccgca
     2941 gggcaggaac tgctcgtcgc aggtcagcag ggttgcacca tgcttggcta aggctgatag
     3001 gagcggggcg gtaatcaggg tttcgcgatt ttcgatgatg ataacggcga tgtcctctaa
     3061 tggtacggtg tgggactcgc cgttttgttg aatcagcagt tgccggcgtt gcaaggatag
     3121 tttgccgccg ttttgaataa gcaggctgcg ccaagtcatg tttttcccct tatttttata
     3181 taaaaaatcc ctgcttcagg ccgcctgaaa cagggataga ttaatttaac ggacaggcgg
     3241 gcgttttttc agacggcatg gtctgatttc tttgcccagt tcgtcaattt ggtatttttg
     3301 gaatgaaagg gcggttttga cgccgatacc ttccagtatt ccatttttgc caattttatg
     3361 atcaagatca tgaatgcgta tattgatatt acctgtgcct cgatggcagc tggcaaagta
     3421 accaaacatt ctagcttttt ttgttataac ctcgactaaa tcattagggt gtaatgagaa
     3481 tttaaagttg aaactatcat caataagttg ccaatcttct tcatcttttc cttgtacaac
     3541 agccctatcc ggcaaaatcc ctttcgctac ctgccaactg taaatcggta ccagataata
     3601 cttgtcgcct ttctcaaaca catctacgcg caccatggtt gcgttgtcgg caataccgtt
     3661 atggttgcgc acccatacgc cggttttctg tacttgctct acgcgtacgg cttttacctg
     3721 ttgggtgcgg ttgcctgctt tatcgtattt gtaaaacggc tcggcaaagg ctttggcagg
     3781 atcgtcttta tgtgcttcca gccgtgcttt cagtgcttcg tatagcttag gttcgcgctc
     3841 ccgattgacc attttttcca agtctttcag ttttaactgt gttaacggta cgcgcaacac
     3901 gctgacgcct tcgtccagtc gtttggcgga tttgacggtc tccatatgcc cttgcccgct
     3961 catcttccga ttgggcgcgc gtgaaacaaa cagtggcgta acgtattcgt gtacggcttc
     4021 ggggcgagat gataattttt cggcaagcaa cgtgcgcagt ttttctgggg tatcggcttc
     4081 ttcgaattcg ggtttgccgt ccggtttgcc gaagacgcga atcatgactt cttgtgcgaa
     4141 aaattcccaa ggttgtggga agtgtgtttt ttgatgcagc acttctcctg tttctttgtc
     4201 tatggtttta ccgtcaaacg cgttcatctc tttatagcgt acaaaacggg taattttctg
     4261 ctgcatggca acggtcgagc aggcaacgac gacggcgtcc aaggcgtgat ggcggtcgtt
     4321 ttccgcacgc actttgcgca atccccaaaa gccgcgcaac agattggtaa tttgtccgtt
     4381 ggatgcaaag acacgtttct tgcctttacc tgtcagccgc atacggtcgg caacaaattg
     4441 acacaggaaa cggttgacgt agcgcgtgtc gttcagattg cgttctttaa agccgtcttc
     4501 atcgaatttt tgcagcagaa tccgttgttt tttactgcgc gggaaacggc tggtttcgac
     4561 acgcgcttta aattcctgcc attcgcggct gttgtctttg ccgttgaagt attcgtaagg
     4621 ggtttgattg cctttgtttt ggttttcgct gcccaatacc agtactttat tgttgaaact
     4681 gtcgtcccat gtgcgcgaga acggcagggc atggtcgatt tcgacatagc ctttttcgtt
     4741 cagacggcct aagttgattt ctttgcccga atacaggcat ttgccgtgtt gttgctcgta
     4801 caggcgcagt ttcagaatat ctttggattt gggttctccg acaaaattgg ggaaatactc
     4861 tcggaatttg gcggcggctt tttccctgtc tttgcggttt tcttcttggc gtttctcaat
     4921 ttctttgcgg tctttaaacg atttacctac ttcccttgca gtttcaatat ggatacgagc
     4981 tggggagccg taacggcgta ccacgccgtt aatgacctta cgtgcttgag ataaggcgcg
     5041 caagacgacg gggttgcgga tttcgtcggc gggaatcggc ggcagataaa tcttttcttc
     5101 cgtattcttc ttgccgtaat ggtctccgta gatttcggcg caggcttcat cgtaacgttt
     5161 gccttgttcc attagaggca caattcggcg caatgctttc aaggaaattt ggacgaactt
     5221 atcgaagctg atgtgtttca acagcgcttc taagatttcg ggctgtatac ggtctttcag
     5281 acggcctgta atgtcttcat cggttttgaa cagggagaat gccgtgccga tttcgtcttg
     5341 taattcggga gaaaggttta atggggattt tttgtctttc aatccttctt tttccagtgc
     5401 acggctgatg gcatggtagg ccttcatttc catcaatgtt gaggcttcgg cattgtcttt
     5461 accatagcgc aagcctttga aaaaggcggt atcttctaaa cccagcagct tacgggcttg
     5521 tgcgtaagtc agtttggatt ttctgtatgg ctcgtccata agcgtggcgc gttcggtatc
     5581 ggtcaatggc cgctcgctgc cttgctctaa aatacgcagg ttgttcagct tggtcagcca
     5641 gatgaaacgt tcggctgtgt aggtgttttt agcggctttc ggctctgccg gttcgaaggt
     5701 gcaatgcccc aacatttttt gaacggcatc gccggacagg gcagggcgtt gcgtcatcag
     5761 tagggtttca ataccttctt taaggccgcc tgaaacatgc ggattgccaa attctttttg
     5821 tttttcaaac agcaaaatca gctccgcctg taaatctttg cggctgaacg tatgcgaata
     5881 atcgccgcgc tgattgcgga tatggccgct ttctttctca aatttattta aagccaattc
     5941 ggccggtgtg cggaaatcgc ctgtctgtaa ggcatgggca ttgtcggcta cgcctttgag
     6001 caaagcgcca agctctttat cggcagtttc gccctcgttt tttctttgcg acaaataacc
     6061 gcggtgtttg attaaatgca acaagactgc cgaccactct aaaggcgtca gtttgcggtc
     6121 taatgcggct gcgcgaagtt gccatggtgt attcggtaag gatttgatca agccgttttc
     6181 gtcaaaatca gcggcttgta atacgccttc gcgtttcaat aggcggcagg cccgaagcag
     6241 gcgatgggcg cgacggcggg tcaggcggcg gacactgcgc gccaaacgcc ttgccatggc
     6301 aagggagtcg cctgttttcg gtacttcggc acgctcaaat acgcgcacgc ccaaatcaat
     6361 caggcagatt gggttttcat cttcgtcaat ttctaccatc gcccagccga cggatgcgat
     6421 gccgatatcg aggccgagga tgtagttgat tggattaggt ttgaaggcag ccatttatta
     6481 ctccgtaaac aacgttgcaa ataatgataa tatatcttat tgtcttattt atatacaaat
     6541 agattattga cttatcatct cacaacggct agaatcccaa acatattgtc gcactgcgaa
     6601 atgagaaccg ttgctacaat aaggccgtct gaaaagatgt gccgcaacgc tctgcccctt
     6661 aaagcttctg ctttaagggg catcgtttat ttcggttaaa aaatgccgtc tgaaaccgat
     6721 tttgggcttc agacggcatt tatattttgg gttaccagtt gacgcgcacc gaccggctgc
     6781 cgagcaatgt ttccagttcg ccgaacaatg cggagctcgg tgtaaccgtc catttcggcg
     6841 gcacttgaag cctgcccgac gctttttcgt tggcatacga cagttgcagc gggatgcgcg
     6901 gcgtgtcggg cagttggtgg gaggagagca gccgtaccag tccgccgatg tcgtgatgcg
     6961 gggcgagggc gaggctgagg ctgcgggcgt agcgttcgcg tgccgtttgc agggtcatga
     7021 cttggtttgc catgatgcgc agtccgtcgc cgccgccgta gtcgtcgcgg ctgactttgg
     7081 attcgataat cagcacttgg tcggctttga ggcagtcggc gcagttttcc agcgtctgac
     7141 cgccgaccat gatttcaacc tgcccgctca aatcttcaag gctgacgaag gcgattttgc
     7201 cgcgcttgcc catcatcgta cgcacggcgg taacgaatcc ggcgaggcgc acgctgtctt
     7261 gcggcttcag acggcctaat ttggtcggtg cgatttggcg gacttcttgg gcatacgggc
     7321 cgaacgggtg gccggagagg taaaagccga tgacggtttt ttcttcggcg agtttttccg
     7381 attcgctcca catcggcgcg tcgatgagcc gcaccggttc gatggcgtct tccatcatgt
     7441 cgaaaagtcc gccctgattg gtgttggcgg ctttttggtc ggcgttgttc atggcgaggt
     7501 cgatgtttgc caagagcatg gcgcggttgg gttcgatgct gtcgaacgcg ccgccgcgta
     7561 tcaaggcttc gagggtgcgg cggttcatgt gttctttgcc gacgcgctcg cagaagtcca
     7621 agaggccggt aaacttgccg ccgctttgcc gcgcggcggt gatggattcg acggcggctt
     7681 cgcccgtgcc tttaatcgcg ccgagcgcgt agcggatttt catgtccgga tacggcgtga
     7741 agcggtagtc ggattcgttg atgtcgggcg gcaggaactc gatgccgttg gcgcggcagt
     7801 cgtcgtagaa atgcttgagc tggtcggtgt tgtccaattc ggacgacatg gtcgccgcca
     7861 taaattcggc ggggtagtgg gctttgagcc atgcggtctg gtaggaaatc agggcgtagg
     7921 cggcggcgtg ggatttgttg aaaccgtagc cggcgaattt ttccatgtag ttgaagattt
     7981 cgtcggattt ttcgcgcgaa atgccttgtt tcgccgcgcc ttcggcaaag atctcgcggt
     8041 gtttcaccat ttcttcgggc tttttcttac ccattgcgcg gcgcagcaga tccgcgccgc
     8101 cgagcgagta gccgccgata atttgcgccg cctgcatcac ttgttcctga tacaccataa
     8161 tcccgtaggt cggcgcgagg atgccttcga gcagcggatg gatgtattgg aattcctggc
     8221 ctttcatgcg cgcgacgaag tcggggatgt tgtccatcgg gccggggcgg tagagcgata
     8281 cgaaggcgat gagttcttca aacttggtcg tatgcgccgt tttcagcatt tttttcatgc
     8341 cggtcgactc aaactggaac acggcggtgg tgttcgcatc gcggaagatt tggtaggcgg
     8401 cttggtcgtc gagcgggatt gtgccgacat cgacgatatc gccggtggtg tttttgatgt
     8461 tgtcctgcgc catttcgata atggtcaggt tgcgaagtcc caaaaagtcg aacttcacca
     8521 aacccacgtc ttccacgtcg cccttgtcgt acatggatac gggcgaggcg gattcgtccg
     8581 cctgatacac agggctgtaa tcggaaatct tgcccggcgc aatcaacacg ccgcctgcgt
     8641 gcatacccaa accgcgcgtc aggtcttcca gctttttcgc cagcgtaatc agttcgtccg
     8701 cttcttccac ctcgatcaat tcctgaatct gcggctccgc cttcatcgcg tcgtccaaac
     8761 tcaaaggttt gttggcttcc agcggaatca gcttggacag cttgtcgcac agcataaacg
     8821 gcagctccag cacacgcccc acgtcgcgga tgaccgcttt ggacgacatc gtgccgaagg
     8881 taacaatctg gctgaccgcc tgagcgccgt atttatcgcg cacatattcg atcacgcggc
     8941 cgcggtttgc ctgacagaag tcaacgtcga agtcgggcat ggagacgcgt tcggggttta
     9001 agaaacgttc aaacagcagc gcgtatttca gcgggtcgag gtcggtaatt ttcagcgaat
     9061 acgccaccag cgaacccgcg cccgaaccgc gtcccggccc gaccggacag ccgtgcgttt
     9121 tcgcccagtt gataaagtct tgtacgataa ggaaatagcc ggggaatttc atctggatga
     9181 tgatgttcag ctcaaaatcc aaacgttcct gatattccgg catttttgcc gcacgctccg
     9241 cctcgtcggg ataaagctga accatgcgtt cctgcaagcc cttgtttgag agtttgatga
     9301 gatagtcatc gcgcgataaa ccgtcgggcg tggggaaaag gggcaggaag tttttgccca
     9361 atgtgatgtg caggttgcag cgtttggcga tttccaccgt gttttccaag gcttcgggca
     9421 agtcggcgaa acgctccagc atcgtttccg gcggaatgaa aaactggtcc ggcgtgaaat
     9481 cgcgcggacg ttttttgtcc gtcaataccc agccgcccgc gatacacacc cgcgcctcgt
     9541 gcgcgttgaa atcgtcgcgg ttcataaact gcgtcggatg cgtcgccacc accggcaaac
     9601 ccagttcctc cgccagcttc acgctgccgg aaacgcaagc ctcccattcg gggcgttcgg
     9661 gcaggcgttg cagctctaaa taaaacgcat cgggaaacca tgccgcatat ttcaacgccg
     9721 ccgcgcgcgc cgcgtcttca ttgccgttca acagattcac gcccacttcg ccgtaatgcg
     9781 cgccgctcaa acaaatcaag ccgctgttgt cgccgtttgc cagccattcg ggattgagtt
     9841 ccgcatgatg gatattgcgg tctttgccga cataggccgc cgttaaaagc tcgctcaagc
     9901 gcagatagcc cgcatcgttg cggataatca gcatggcgcg aaacggcttg tcgggcgcat
     9961 tcggattgcc tatccatacg tccgccgcgc cgacgggctt aatccccgcg ctgcggcagg
    10021 ctttatagaa tttcaccaag ccgaattcgt tcatcaaatc gctgatgccc aaagcaggca
    10081 aaccgtattc ctgcgctttg gcaatcagtt ttttaatccg caccataccg tcggtaatcg
    10141 aaaactcggt atgcaggcgc agggggatgt aggtcggctc ggtcatggca aaatcggcgt
    10201 gaacaataaa aggcgtattg tagcagggtt gtctttagat ggcggtgtag gtcatgccgc
    10261 ttcggattca gacggcatga cctgcaaatg tttttgagct tttacgacgg caaaaaaatg
    10321 cctcctgccg tatggcggag gcttcccaag gagtattgat agatataaag gactatcaaa
    10381 ctagttataa agaactatat accttattcg gacggatggc aagcagttaa attaatttta
    10441 cgttcaaaca ggtttttgat ttcgttttga tggctgatgc cgatgaccag tgtatcgggc
    10501 agttcgtgtt tgaggatgtg catcagcgtc aatgcggatt cgtcggggaa caggatttgc
    10561 agtctgccgt tggacaggat ttctttgatc ggatgacggc atatggggtt tcagacggca
    10621 tttttacgga acaggcgggg cgggatgtgc tgcctgtttt gcggtaattt tgggtacggc
    10681 ggcggcaggt gctacaataa cggcctcttt ctgcaaggga acattatgga agccaccgtc
    10741 tatctcgaag acaacgaata catcgccctg tgcgacctct tgaaattggc cggacttgcc
    10801 gaaagcggcg gacaggcgaa ggcgttgatt gccgaagggt tggtgtcgcg caacggcgaa
    10861 accgaaaccc gtaaaaccgc caaaatacgc ggcggcgaag tcatcgagtt tgacggtgcg
    10921 cgcttggaaa tcgccgatgg atatgaccct gaagcataaa gccgaagccc ttttgggcga
    10981 gccgctttta gacgaacccg tccgccccga gtcgtgggaa tgctgcggca gcgactgcgg
    11041 cgaggcgtgc attcagacga tttactgggc ggacaaggcc cgctacgacg cgcaacggcg
    11101 gaaattgaaa gaagcgggtt ggtcggaaga cgaaaatgcc gtctgaaaac ggttcggctt
    11161 gataaaacca cgttttcaga cagtctttta tagtagatta aaattgaaaa cggtcatatc
    11221 gccattccct cgaaagcagg aatccggaag tttgaaatag cggttaacct taaacatttc
    11281 caataaatca aagtctggat tcccgcctgc gcgggaatga cggtataggg actttttctt
    11341 tgcatttgcc atacacctgt tttttaaaat gaccgctgcc gccacactgc ccgacaacat
    11401 tgaaacatcc cgaccttcat tcacaacaca cataacaccc tatgatagac ctgcactgcc
    11461 attccaccgt ttccgacggt atgctttccc ccgccgaagt cgtgcgcctc gcgcatcaaa
    11521 acggctgcac gctgctggcg ttgaccgacc acgaccacac cggcggcatc gccgaagcgc
    11581 gcgccgaagc cgacaggctc ggtttgcgcc tgattaacgg cgtggaaatc tccgtaacgt
    11641 ggcgcgggcg taccatacac gttgtcggtt tggattttga cgaaacagac gaaaacctgc
    11701 aaaacttgtt ggcgcaagtg cggcaaggcc gtctgaaacg cctcgaagcc atcgccgcca
    11761 agctcgaaaa gaaaggcatc ggcggcgcat acgacggcgc gctcgcgctg gcggcaaaca
    11821 aagaaatggt cagccgcact cacgtcgccg agttcctcat ccaagcggga catgtcaaaa
    11881 acaagcagca ggcgttcacc aaatacttgg gcgacggcaa atcctgcgcc gtccgacacg
    11941 aatgggcgac gctggaagac tgcgtctccg ccgtcaacgg cgcgggcggc atggcagtca
    12001 tcgcccaccc catgcgctac gatttgtccg ccaccgccaa gcgcaatctg tttgaagaat
    12061 ttaaaaacct cggcggcgtg ggcatcgaag tccacagcgg caactgctgc aaaaacgacc
    12121 gcctcaacta cgcgcttttg gcagaacgct tcggtatgct cgccagcgcg ggcagcgact
    12181 tccaccgttt aaacgacttc agcggcggca ttctcggcgc gtgtcccgag ctgccggaaa
    12241 actgcaaacc ggtgtgggcg catttttccc gacattgaat gcggataaaa atgccgtctg
    12301 aaaacctttc agacggcatt tttgcggttt aaacgttgtc gtacagtttt cggacggttt
    12361 taccgatggc ggcaatgcct ttttccagcg tttgagcgtc ctgcgcgatg ctcatacgaa
    12421 tgcactcgcc cgcgtgcggg taatcctgcg tgtcgatgcc gacgaagaaa tgttcgcccg
    12481 gaataatcag cgtaccttcg gctttgagca tttcgtacag ggtttgcgaa gaaacgggca
    12541 ggttttcaaa ccagagccac aggaaaatcg cgccttcggg tttgtggatt ttcatcgggt
    12601 acgcgcccag ctcgcgcttg agcagcgaga cggcggtttg cgcctgattg cggtaaaacg
    12661 gccggatgac ttggtcggca agccgtttca tctcgccgct ttccagtagc ggggcggcga
    12721 tggccgcgcc gaagcgcgtg ggggcaaggt tcacaatcgc gttcaggctg ctgacggctt
    12781 tgaccacttc cggcgcggcg acgatgatgc cggtgcgaac gcccggcagg ccgactttgg
    12841 aaaggctgaa gcagaggatg atgttttcgt gccaattcag cgttacgtcg ctgtaaatga
    12901 tgttggggaa cggcattccg taggcgttgt cgataatcag cggaatcccg tgttcacgcg
    12961 ccaaagcgtc caaacgtgcc atttcgccgt cggtcaacac attgccggtc gggttggtcg
    13021 ggcgcgaaca gcaaatcgcg ccgattttgc ccgctttgag ttcgggcagg ttttccagtg
    13081 cgtcaaagtc cacgcggtat ttgaagaagc ctgcttcgcc ttcgtgttcg acgttttcga
    13141 ttttgggctt gacagaaacg aagtgctgcc cttcgatatg tacgtcggca tagccgatgt
    13201 attcgggcgc gagcggcaac aaaatggctt tttctgcgga tgtgccgtct gaaaggttga
    13261 atttgccgcc gaagaggttg aataaataga aaaacgcgtt ttgcgaaccg ttggtcagcg
    13321 cgatattgtc ggcggtcaaa ttccagccgt attcgcggtt gaggaaggcg gtcagcgcgt
    13381 caatcagcgc ggcatcgcct tggggattgg agtagttgcc gatgttttcg acggcgtgtt
    13441 ccgccgccag tttggagaat atgtcggcga acgcccgatc gatttccgga atgcgcgccg
    13501 gattgccgcc gccgagcata ttgacgggct tgtcgctttt gagcgcgtcg ccgaggtcgt
    13561 ccatcagttg gaggatgccg ctgtgttgcg tgaatttttc gccgaatgcc gaaaactgca
    13621 tatcaaactc cgtgtgtgtg tcaggcaggt tggtttgtac gttatgccgt ctgaaggttc
    13681 agacggcatt ttttctgtgt gtttctcgta cccaaagcag gtcgcagata ccgtcgcttg
    13741 gggtaaagcc ggtcggggcg ttgatgagca gggtgcggat tgcctgctgg tcgtaacggt
    13801 cgcaggccgc acggatgtgg tcgagcagga cagagagctc gtgccacggc agcatcgcct
    13861 cgtcagccgt cataatgcgc ggatggccgg ttttgcggac gttgtcgccg atgagcagct
    13921 cttcgtagag tttttcgccg ggacgcagtc cggtaatgag gatttcgatg tcgccgtcgg
    13981 gttgttcggg tgttttgggt ttgaggccgc ttagggtaat catttggcgg gcaaggtcga
    14041 tgattttgac ggattcgccc atatcgagga cgaatacgtc gccgcccgta cccatcgcgc
    14101 cggcttggat aaccagttgc gccgcttcgg gtatggtcat gaaatagcgc gtgatgtcgg
    14161 ggtgggtcag ggtaatcggg ccgccttcgg caatctgttt ttcaaacagc gggacgacgg
    14221 agccggacga acctaaaaca ttgccgaaac gcaccatgct gaagcgggtt ttttgtccgg
    14281 gttcggcggc gagtgcctga aggcagagtt ccgccatgcg tttgctggca cccatggtgt
    14341 tggtggggcg gacggctttg tcggtggaga tgaggacgaa agtttccacg cccgaagccg
    14401 tggcggcaag cgcgcactcg agtgtgccga agatgttgtt gcgtatgcct tcgacggtgt
    14461 tgaactcgac catggggacg tgtttgtagg cagcggcgtg atagacggtc gcaacggaaa
    14521 aggcggtcat gacgtgttcg agcagcgtgc ggttttgcac cgaaccgaga aagggcagga
    14581 tttcggtgtc gaggcgtttt tggatgcagg tttcgcgcaa ttctttttcg atggcgtaca
    14641 gggcgaattc ggataactcg aacagcagca gcttttcggg gcggcggcgg ataatctggc
    14701 ggcagagttc cgaaccgatg gagccgcccg cgccggttac catgacggtt ttgccttcga
    14761 tgtcggcatt catgaggcgg tcgtctggtg cgacggaatc acgcccgagc aggtcggaca
    14821 cagagatttt tttgagcgtg ccgatgctga ttttcccgtc catcaggtct ttcattccgg
    14881 gaatggtcaa cacttcgcac ggataggctt ccagtttgtt gatgattcgg cggcgttgtt
    14941 cctgggtcgc gccggggatg gcgagtagga ttttttccac gccgtagcgt tcgatgagga
    15001 aggcgatggc atcgggctgg taaacggcaa ggtcgtagat gacggtgtgc cacagtttgg
    15061 ggtcgtcgtc cacaaaggcg gcggcggaat attcgcgcat ttgtttgacg gcctcaagca
    15121 gttgtctgcc cgaccgtccc gcgccgtaaa tgatgacggg gatcatctgt tttttggggt
    15181 gttcggacaa cagtccgcgc aaaaccatac gcgagccggt cacggaaaca aacagcagta
    15241 agaaatagac aatcggcagg gcgaggcgca gcctttcttc aaaaatcaat gtgttgagga
    15301 agaacaggac ggcggaaaca atgctgccgg caaaagcggt ggtcaggacg tggaaactga
    15361 caaagcgtgt aacggcacgg tagagcccca ttctgacgaa caggccgatg gtcaacagcg
    15421 cggtcaatag gaaagactgc cagttggcag ggtcgaacca ttcgttcgag tagtcggcct
    15481 tcaggctttg ggtaaaccaa aaggcaatga aaatcatcag aaaatcgtgt atgaggaaac
    15541 agattttctt gatgttgcgc ggtagggcaa gcagggtttc cagagtcata tcgtggggcg
    15601 gtatgtgttt tcaggcggca tatgccgtct gaagggttat cgtgcggctt cggtcaagac
    15661 ggcttcgatg tgttttttgc aaaacgcaat ttcgtcgtcg gtcagcgtcg ggtgcaccaa
    15721 gaacatcagg ctggtgtcgc ccaactcgac agcatttgtc aaacgctctt tcggtcgcca
    15781 cggcgtgttg tcgaaggctt tttccaaata gacttcggag cagctgcctt gatagcaggg
    15841 gactttgcgc gcgttcagtt cgccgacgat gcggtcgcgc gtccagccgt ctttgaggtg
    15901 ttcgggtttg acgaaggcgt agaacttata ttgcgcgtgt ccgatgtagt cggcgacttc
    15961 aaccaagcgg atacttttga atttgcctag gctttccgcc agcttggcgg cgtgcgcttg
    16021 acggcaggca gtccattcgg gcaggcgttt gagctggatg cgcccgattg ccgcctgcat
    16081 ttccatcata cgccagtttg tgccgaaact ttcgtgcagc cagcggaaac cgggcgcgtg
    16141 ttcgcggtgg tacacggcat cgtagctttt gccgtggtct ttgtacgacc acattttttc
    16201 ccacagggtt ttgtcgttgg tcgtaaccat accgccttcg ccgccggtgg tcatgatttt
    16261 gtcttggcag aacgaccacg cgccgacgtg tccgatagag ccgacggatt tgcctttgta
    16321 ttttgcgccg tgcgcttggg cgcagtcttc gattacccaa agattatgtt cttttgccaa
    16381 agccataatg ccgtccattt cggcgggcat accggcgagg tggacgacga tgacggcttt
    16441 ggtagtcggt gtcagcgcgg ctttgacggt ttccgcgctg atgttttggc tgttcaaatc
    16501 cacatcggca aacaccggat ttgcgcccgc gttcacaatg caggacgcgg aagcgaggaa
    16561 ggtgcgcgag gtaacaatca catcgtcgcc ttggccgatg cccattgctt tgagcgcgac
    16621 atcgagcgcc agcgtgccgt tggcaagggc gacggcgtac cgcgtgccgg caaaggcggc
    16681 aaattctttt tcaaattcgc ggcattcgtt gcccgtccag tagttgactt tgttggacag
    16741 caggactttg gaaacggcat cggcttcttc ttgggtgaag cagggccacg gggaaaggaa
    16801 agtgttcagc atgatggttt gtccgtcggt tttcagacgg catttccgac cctatgccgt
    16861 ctgaaggggg gcgtgttccg aagaatcggg cgcgcgccgc aggtgttgtc aaaatcggtc
    16921 tgtacgaggg tgtattttaa tcgcttatgc tgtcgaggtc tcggggtttt tgcgcggcag
    16981 cggctttgcc ggattgcccg cgacggtcat gccgtccggg atgtcgcaca cgataaccgc
    17041 ccccgcgccc attcaacttc catcgtttcc aaatggtgtc gaaaacgtag ggcggaaagc
    17101 cgtttacgcc ccatgaataa tattttgtca tgaaatagtc cttttaatat tgtgagtgca
    17161 tgtttgatta tgtaatggtt aagtatttga atcaattata ggaactaaat cttgaaagga
    17221 gttaacccaa aagtcgggat gatattctgt gttaaaactt tcaggctggt ggcggtggat
    17281 gttctgtccg gagggaagca atcctatggt tatccagccc aaagtgttcg gcgcgataaa
    17341 gtctttggaa atgttgtcgc cgatatagat gaagcagcct gccttgtcgg catatttgtc
    17401 ttgaagatgg cggaagcgtt tgccgtcggg cttttccgaa gagcaggctt cagagatgag
    17461 gatgtcgtca aacagggaag acagacccaa ggcttccagc ttcaggcgtt gggtcaggct
    17521 tcttccgtcg gtaatcagcg cgcgtgcggc gaagggcgcg gtcagttcgg acaggaaatc
    17581 cggcggagca tacggagtca gcgtcgggcg gtgcagccgg tattgccaca aaagaacctg
    17641 tttttccgat tcattaaaac cacagtggcg gcacaatttg tccagccaat ctttgccgtc
    17701 ggggtcgatg ttgcgccata ggttgtctga attccaatcg ggatacagtg cggcaacggt
    17761 atcgatgacc gaacggatac cggagagctt gtattcgtat tccgaataca gggtatcgtc
    17821 caagtcgaag acgatgacgg tggtttcagg attaatcgtt ttcatgaacc aagaccttgg
    17881 catcgtagcg caacataatg aggttgtttt cccagtcgtc tgaaaacggg atttcttcgt
    17941 cgccgaggta ttcgcgcatc aaccggctcg gataattgcc gcctgcggca tacgtcagcg
    18001 ggaagccacc gccgaagcgg gggttgattt ccacgccgta gaattcgccg gtttcttcgt
    18061 tgtagaagaa ctgcgccgtg atacagccgc gcgcgccctg caataccgcc attttttcca
    18121 gcagcgtttg gtagaggctg tttttgcagg taacgccttt gctgatttcg ccggagcgca
    18181 cttccaagcg ttttcgcggg atggcgcatt tcagacggca ttgacggtcg taatacatat
    18241 cgacggtaaa ctcggaaaat ttattttcga tgtcgataaa ctgacagaac attaatttgg
    18301 tgtctgccaa gacttccggt gtcaggtcgg caggtgtgtc aatccgtttt gcaccgatgg
    18361 cgcggctgcc gtcataaggc ttggcgaagc aagggaaaac caacttcttg cggtcgtaaa
    18421 tttcgggtga gcgtatgccg tagcgggcaa acaaggtggc ggttttccgt ttgtcgcggc
    18481 attgtgcgat gaagtccgaa tcggaaacga cgatgtggac acttgccgct tcaaagcgtt
    18541 cgcgctcgtc tgccagcttt tgcagttcgg tgtctatggt tgggatgatt aaaccgatat
    18601 tttcgcgtac cgccagattg aaaatgctgt cgatatatga atcggtacta atcggcggca
    18661 cttcaaatga accgtccgaa acatggcagg cagaagacat gcgggggttg aggtcagtgg
    18721 caaaaacgct gatgccgtct gaaaaacgtg cggcttcggt ttggaagtcc tgcaccaatt
    18781 cgacacggcg acctgccgac agaatcagta tattattttt ttcattagtt tgatttccag
    18841 tgaaataagg catagtggct tccccttccg ccgaaatccc ttctttgata aagacttttc
    18901 tgactgtcaa aaacaggatt ttcatatcca gccaaaaact gtaatggtcg atataccaaa
    18961 catcgcaggc gaatttttcg tcccacgaaa gcgcgttgcg cccgttgacc tgcgcccagc
    19021 cggtaatgcc gggtttcatt tcgtggcggc ggttttggaa gttgtcgtac agcggcagat
    19081 attgcatcag cagcgggcgg gggccgacca ggctcatctc gcctttgagg atgttccaca
    19141 gttcgggcag ttcgtccaaa ctggcggcac gcagtttttt gccgaaatcg gtcaggcgtt
    19201 cgctgtcggg cagagggttg ccgtctttat cgattgcatc gcgcatggag cggaatttaa
    19261 tcattttaaa cggcttacca tccttgcccg gccgctcttg gatgaagaac acgggcgagc
    19321 ctaggttttt gcggatgagg tacaccaaaa tcaaaaacac aggcgacagg ataatcagcc
    19381 ccgaagcgga ggcgacaatg tcaaacaggc gtttgaaaaa ttgattcatg agctaatctt
    19441 tcaatcaggt tgacgatttt ttgataggca acgtcgcgtt tgaagcggcg gacgatttcg
    19501 tcggactgaa cggggtcgtt tttgcgtttc aaaatatctt tggcggcttg aacgaagctg
    19561 tccacgtcgc cggaacggta gttttcgtgc ggtagtaggt tcaagaggcc gaggacttcg
    19621 gcattggtct ggctgtttaa aatcggtttt tgcaaagcca tatagtcgga gagtttgttg
    19681 gtaaccgact gcatggcgtg gctatggatg gcgttgacgg caatgtcgca cgctttggca
    19741 atcgacatca tttcagaata gggtaaataa ccgtaaaact tgatggcgcg attttcatat
    19801 tgtttgagtt tctccaaatc gggtcctccg cccataatgt gcaattcgac gttttcgccg
    19861 tcgtctaaaa gtttccgaac gcctttgcac acggtttcca cgtcgtagct gtaactgagc
    19921 gtgcccaagt agaaaaggcg gacggttttg gagcggaatc gcgggggggg ggggcgatgg
    19981 cggcaaaatc cgtaccgata tagacggttt cgccgggtac gttcggattg gcttctttgg
    20041 cgcggtcgag ataagtctgc gatacggcaa tcagcgcgtc ggcgcagcgg taggcacggt
    20101 tggcgcgtga agcaaagggc agcagcttgt gcggtacttt tttcaaaaac ggtacgaccg
    20161 aggagaaaga ctccggccat acgtcctgca catcgacaat caatttgtag cccaaacgcg
    20221 ctttgtgttt gcccaacagc aggttggttg ccatcagcgg ataggcggaa tagacgatat
    20281 cttgttccgc cgcctgcggg ctgtgcagcc agcgtgccaa attgcggacg aatacgcggt
    20341 ggctggcgac gcgggcgagg gagacgtttt ttcggtagcc gggttcgtcc agcagcttga
    20401 cgtgcagcct gccgtttgaa gtggcggcag catcttcatg ccgtctgaag gatttgtcgt
    20461 agtggcggaa acggctggta atcagcaaca cgtcgtgcga ttgcgacaat cgttctgcca
    20521 gataccaaaa gcggttgaaa tacggttcgg acggcagcga gcagtagggg gcgactatgg
    20581 tgatgttcat cggcggcttt cagacggcat tacatggtat aggcggccat ggtggtgcgg
    20641 tagatttcgt cgtcggaaca gagtgcggcg acgtgtgcgt gcagcctttt gcccattgct
    20701 tcgcgcaact ccggttgtct gacgagcgtg tctacggcgg cgataaaggc ttcttcgtcg
    20761 ccgaagggga tgcagtaacc cgtttccccg ttgacgacca tttcggaaat gcccgccata
    20821 tcgtaggtaa cgacgggcgt gtcgtaaagt ccggcttcta agatgttgtt gcctacacct
    20881 gcgccgtggt cgccgatgca gtggggcgtg ttcaccaaaa tatccacgtc tttgaagtag
    20941 gcggtcaaat cgcgcacgcc gccgaggaag gtgactttgt cttcaatacc caaacgtttg
    21001 gcttgggctt tgaggttgtc catttcttcg cctatgcccg ccacgttcag gcgtacgggc
    21061 atatcgcggt cgaccatttt tttgaaaatg tccagcatca gatgcacggc gcggacggtg
    21121 ttcaaacggg aaagcgtgcc gagtacggcg cattcttttt tggttttccg gaaatgaaat
    21181 tccggggggg gggggagtgc gttgtaggcg taggcaatcc ggccggcggg gaagccgtgg
    21241 cggatgagtt tttcgcgctc gtgtttacag ttgccgatga tgtacacgcc cagcttgtcg
    21301 aagagcttgg cgattttggg gtaggttgcc gcgtccaaac cgtgcgagtg gtagatgatt
    21361 ttggttttcg gcgaaacgat tttggcggca agggcgcagg ctggaacaac gcgcgccatt
    21421 tggcagtcga tgatgtcggg ttgttcgcgg cgcagcatcc gggcgtaggc aaaggtgctt
    21481 ttgaggtagc cggcaagccc gccccggtaa aaatcaaccg gctgccaacg gatgcccgat
    21541 gcttgcgcct ctccgacaaa aggcccgtcc gaagaagcaa ggatgatgtc gtgcccgcgc
    21601 cgtttgagca gccgcccgag gcggacggtg gcggtttccg tgccgcccaa gcccgacata
    21661 gaagtggtca ggatgatttt cataatggga aaccttgttt tttcaaataa tgaaacagtt
    21721 tgtgcaaatc tttccggtgg cgcaggatgc agcctgccag atatgccgcc catacgccgg
    21781 caaacagggg atagtttgcc ggcgtgccga agcaggtgta ggccgccgag gaggtcaggc
    21841 agaacaatgt gtgcagataa agcggcaggc gtttgagcgg ctgccacagg cggcaggagc
    21901 tttcggtctt gaaaacaaaa aacagccaaa atgaggcggc acaggcaacc gccgcgccgc
    21961 gcgcgccgcc ggacggtacg gcaagcccca gcagcagcag gtttgccgcc agcgcgccca
    22021 aggtggcgag cgcgatcggg cgtgtttttc ggacgacgtt caaaccgatg ccgctgattt
    22081 ctaccagcgt gcaaaacagc ggcggcagca tacacgatac gacgataaac cggacggcgg
    22141 cgtagttttc cggcagcagg agggaggcga ggggcgagaa aatgccggtc aggcagaggg
    22201 cggaggcaag cagggcggcg gcggattctg ccgttgccga gaggcgggcg ggcggggcgt
    22261 ttgcttcgat tgcgcggaaa atatacggtg tccagaccgt tgaaaagatg ctttggaaca
    22321 ataatgccgc tccgccgaac gaaataccca tcgaataaac gccgagctgt tctaggccgg
    22381 catatttttt caggaacaaa cggtcggcgg atgccagccc ccaataggcg atgctgctca
    22441 gtgcgatcgg tatgccgtag cgcaggccgc gatgcaggac ggcggatgaa aacggtgcgc
    22501 gccggacggc cttcagacgg catcggtttt gaaacagcaa aaaggcggcg gcggcaaggt
    22561 ttgccagcgc gtaaacggcg gtcaggacgg cggtgttcgc tggaaagtgc agcagcccga
    22621 ccgtcagcgg cagcagcagc aggatggcga gcttgggcac gagttgcgcg gacgaaaagg
    22681 caagggcgcg tccttccata cgcaaaacca gtaagagaaa gcggatgggc aggaagctca
    22741 gttcaaacag caccagcccg atgccggcgg cggcatcgtc gagtgaaaac aggatttcag
    22801 acggcaggga cgggcgggaa agcagcaggg cggctatcgc ggcggcagac agcagcggcg
    22861 gcaggaacag ggttttgaac aaggtgtctt tgtcggcggc ggcatagtat tcgcggacgt
    22921 atgcctgatc cagcccgagg cacaacaccg acaccgtcag ccccgccgcc gtctgcatca
    22981 gcacgatgcg cccgatgtcg tcggcgggga aataccacga cagcagcggc aggatgatga
    23041 cggctaaaac cgcgctgccg atcgagcctg ccgcgtagcc gaggatttct tttgtgtcca
    23101 tttttgatgt ccgggcggcg gcgggatgct gcctgtgccg tctgaagcct ttcttgatcg
    23161 gaatttgacg gctttcagtc cgttgcggct gccggcgggg tgcggcagtc cgggttgcgc
    23221 ttttccgggc gggcggcggt ctgaacgggg ccgtttttta tcggcgttat tatatagtga
    23281 aacggcgcaa accctttaaa aggcgttatc gtttttccgg aacacggttt tgatgtcgtg
    23341 tccgaggatt tcgcttgaaa cgggtgtcca caagggcggt gcggaaaggg cggcgcggtt
    23401 ttcgggcagg caaaataaac cgctgccgcc gccgaggatt ttgggcgagc ggtataaaac
    23461 gatttcgtcc gccaaatctt ctgccaaaaa tgcggatgtg agttcggagc ctgcttcgac
    23521 catgatttcg ccgaaacctt cgtcggcaag gaggcgcatc aggtggtgca ggtcgatttt
    23581 gccgtctgcc gtttcagacg gcatcaggat gcggacgtgt gcgtgttccc gataggggtg
    23641 cagtctgtct tcgttgcgtt cgagtgtggc gatgtaggtc ggagattgtc cgtcggtaac
    23701 caaatggctg ttcgggggca ggcgcaggcg gctgtctaaa acgatgcgtg cgggttggcg
    23761 caaagttgga aaagcgcgga cgttgagccg gggattgtcc gccaacaccg tgccgatgcc
    23821 ggtcagcacc gcgcagcttt cggcacgcaa aacctgtacg tcggcacgcg cgtcttcgcc
    23881 ggtaatccaa aagctgctgc cgtctgaaag ggcggttttg ccgtccagcg aaacggcgca
    23941 tttgaggcgg acaaaggggc ggcggcgttc gatgcgcgac aggaagcctc ggttgagttt
    24001 ccttgcctga tgttcgagta aaccgcattc cgtcttgatg cctgctgctt cgagcagggc
    24061 aagccctttg cctgcaacca gcgggttggg gtcgcgcatg gcggcgacga cgcgtgttac
    24121 gccggaacgg agcagagctt cggcgcaggg cggtgtgcgt ccgtaatggc tgcacggttc
    24181 gagggtaaca aaggcggtcg cgccttgtgc catttcgccc gcctgacgca gggcgtggac
    24241 ttcggcatgg ggttcgcccg ctttgacgtg gaagccctgc ccgacaattt ggctgccgtg
    24301 tgcgataacg cagccgacgc gcggattggg cgaagtggaa aaacgcccca aagcggcaag
    24361 tcggagggcg gtttccatca tggatatatc tgtgtccgaa aacataggga taccgtatca
    24421 gtatgggttg ggggaatcag gctttgccgc ctgttttgac ggcttgcgcc agccacgagg
    24481 cgaaatgttc cgggctgtcg aagcgtttgt gcagtgcggc gaagcgtatg gcggcttccg
    24541 tgttgtgctc aagcagctct tttagcacca tgtcggcaag ttcggtggag gggatgtcgc
    24601 gctgacccga agtgtagagc ctgtgttccg tcaggcggac ggtttcgtcg atctgttcgg
    24661 gtgtcagggc ggattttcgg gcggcggcgg tcaggtcgtt gcggaggcgt tgtgcattaa
    24721 agggcgaacg ttttttgtcc ggaccgatga cggcgggcat tttgagttcg gcggtttcga
    24781 gcgtgccgaa gcgtttgccg cagctggggc agtggcggcg gcggcgcacg gcgttgcgtt
    24841 cttccatcag gcgcgaatcg gcgacgcggg tgtcggggtg ggcgcaaaac gggcatttca
    24901 tagcgtttct tcctgatgcc gtctgaacgt caaaccgata cgccggcggc gcgggcgatt
    24961 tccagacctt cttcggcact catatagacg gggttttcgg gacggtcgtg ttggacgatg
    25021 ttgccttcgc ggaacatgac cagttcgtcc acggcaagtt gggaccatga ttcgtcgcgg
    25081 gtcagcggca gggtggcgat aacggcgacg cggtcggagg gcgtggttac ttcggcaaaa
    25141 tcgaccatca cgtcgtcgtc gagcaggcgc gccttgccga acggggcttg gcggacgatg
    25201 tagtgcagca gtgtgctggc gtgggcaaac agggcaatgc cgtctgaaag cataaagtta
    25261 aacagcccga acttgcggat ttcgtgcgtc agccccgcaa tcgcgtcaaa cagcgtgtcg
    25321 tcgtcgggac gggcggcaaa gcgggtgcgc aggcggttga ggatgtggca gaacgcgcgt
    25381 tcggaatcgg ttgtgccgac ggggtggaaa aattcgccct gttcggggaa aaaatcaatc
    25441 aaatgtccgt tgtgggcaaa cagccagtag ccgccccaca tttcgcgcat aaagggatgg
    25501 gtgttcgcca gcgaggtttg tccttgtgat gctttgcgga tatgggcgac gacgttttcc
    25561 gatttaatct ggtaggcgcg cacgaggtcg gcgacgggcg aattggcact gggtttgtcg
    25621 tcatggaaca ggcgcacgcc tttgccttcg aaaaagccga tgccgaaacc gtcggcatga
    25681 tggtcggtaa tgccgccgcg ccggcggaag ccttcaaagg aaaacataat atcggtcggc
    25741 gtattgcagt tcatgcccag cagttgacac atggtttgtc caaatgattc agatggtcgc
    25801 aagtattcgg attatacccc gaactgaaaa tgccgtctga aatacggctt gttccccatt
    25861 attcccacga aaacagaaaa caaagacgga aacttaagat t
//
LOCUS       DAWKQJ010000009        24256 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000009 DAWKQJ010000000
VERSION     DAWKQJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.009"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(248..964)
                     /gene="trmB"
                     /locus_tag="WJL20_000291"
     CDS             complement(248..964)
                     /gene="trmB"
                     /locus_tag="WJL20_000291"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ7682491.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1086..2489)
                     /locus_tag="WJL20_000292"
     CDS             complement(1086..2489)
                     /locus_tag="WJL20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7682492.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EERLPEAQVRLMYLLYASRHFEEALEWAKTSTKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(2607..4493)
                     /gene="uvrC"
                     /locus_tag="WJL20_000293"
     CDS             complement(2607..4493)
                     /gene="uvrC"
                     /locus_tag="WJL20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ7682493.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEQVEGISKALAETIY
                     NHLH"
     gene            complement(4548..6725)
                     /locus_tag="WJL20_000294"
     CDS             complement(4548..6725)
                     /locus_tag="WJL20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ7682494.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            6864..7814
                     /gene="trxB"
                     /locus_tag="WJL20_000295"
     CDS             6864..7814
                     /gene="trxB"
                     /locus_tag="WJL20_000295"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ7682495.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            7970..8338
                     /gene="rpsF"
                     /locus_tag="WJL20_000296"
     CDS             7970..8338
                     /gene="rpsF"
                     /locus_tag="WJL20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ7682496.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8339..8641
                     /gene="priB"
                     /locus_tag="WJL20_000297"
     CDS             8339..8641
                     /gene="priB"
                     /locus_tag="WJL20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ7682497.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            8648..8878
                     /locus_tag="WJL20_000298"
     CDS             8648..8878
                     /locus_tag="WJL20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ7682498.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8895..9347
                     /locus_tag="WJL20_000299"
     CDS             8895..9347
                     /locus_tag="WJL20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ7682499.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            9361..9468
                     /locus_tag="WJL20_000300"
     CDS             9361..9468
                     /locus_tag="WJL20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ7682500.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            10080..10307
                     /locus_tag="WJL20_000301"
     CDS             10080..10307
                     /locus_tag="WJL20_000301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682501.1"
                     /translation="MGGSRIAWNQHGSRIAWNRHGRRCADAFGREGGAIGSLEKGNGI
                     CGQKKLRVKVGRTVRLSVLLSEQEAGQLCPD"
     gene            complement(10335..11141)
                     /locus_tag="WJL20_000302"
     CDS             complement(10335..11141)
                     /locus_tag="WJL20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7682502.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYTQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGPQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11142..11888)
                     /gene="pssA"
                     /locus_tag="WJL20_000303"
     CDS             complement(11142..11888)
                     /gene="pssA"
                     /locus_tag="WJL20_000303"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ7682503.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12471..12953
                     /locus_tag="WJL20_000304"
     CDS             12471..12953
                     /locus_tag="WJL20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ7682504.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(13005..14216)
                     /locus_tag="WJL20_000305"
     CDS             complement(13005..14216)
                     /locus_tag="WJL20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ7682505.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLTGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14439..16877
                     /locus_tag="WJL20_000306"
     CDS             14439..16877
                     /locus_tag="WJL20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ7682506.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            17085..18395
                     /locus_tag="WJL20_000307"
     CDS             17085..18395
                     /locus_tag="WJL20_000307"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ7682507.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18491..19105
                     /gene="clpP"
                     /locus_tag="WJL20_000308"
     CDS             18491..19105
                     /gene="clpP"
                     /locus_tag="WJL20_000308"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ7682508.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQL"
     gene            complement(19159..20424)
                     /gene="ispG"
                     /locus_tag="WJL20_000309"
                     /gene_synonym="gcpE"
     CDS             complement(19159..20424)
                     /gene="ispG"
                     /locus_tag="WJL20_000309"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ7682509.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIASEFL
                     AIVEEYVKTNYGENSSKRNKGKVIPIQSL"
     gene            complement(20440..21201)
                     /gene="pilW"
                     /locus_tag="WJL20_000310"
     CDS             complement(20440..21201)
                     /gene="pilW"
                     /locus_tag="WJL20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ7682510.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(21204..22298)
                     /gene="rlmN"
                     /locus_tag="WJL20_000311"
     CDS             complement(21204..22298)
                     /gene="rlmN"
                     /locus_tag="WJL20_000311"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ7682511.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMIPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22442..22867)
                     /gene="ndk"
                     /locus_tag="WJL20_000312"
     CDS             complement(22442..22867)
                     /gene="ndk"
                     /locus_tag="WJL20_000312"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ7682512.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(22926..24077)
                     /locus_tag="WJL20_000313"
     CDS             complement(22926..24077)
                     /locus_tag="WJL20_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ7682513.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
BASE COUNT         5644 a         5955 c         6690 g         5967 t
ORIGIN      
        1 ccagcaagcc gtagcctaca tcacaccaac cgcgtgcgta gctatcacat actacctgcg
       61 ggcggcacaa actttaaaac cgatgtgcag gttaaaacct acaaggatgc gtgcggtaca
      121 cacgcgtttt gctgttcgca ccattgcttt gaactttata tcgaaacatc actcgctgct
      181 ttatcttgaa aaaatgccgt ttgtaagcaa acatgcttca gacggcattt ttttatccgg
      241 cggtttgtta tccgatccgt ttgaatacca agtcccaaac gccgtgtccg aggcgtttgc
      301 cgcgcgcttc gaatttggtt tcggggcggt agtccggggt gggggcgtag tctgccgccg
      361 tattttgcag gctgtcgaag ctgctgagga cttcaagcat ctgctgtgca tattcttccc
      421 agtctgtcgc caggtggata tagccgccgg ttttgagttt gggcagcagt ttggcgatga
      481 agggggcttg tatcagacgg cgtttgtggt ggcgtttttt gtgccacggg tcggggaaga
      541 atatgtggat gccgtcgagc gagccgtctt gcagcatatt ttcgacaact tctacggcat
      601 cgtgccgcat cacgcggata ttttccaaat ggttttcgtc tataagtttg agcagattac
      661 ccacgccggg accgtgtacg tcgatggcga gaaagtcggt ttcgggcagc ctgcgggcga
      721 tttctgcggt tgctgtcccc ataccgaagc ctatttcgag gattttgggc cggttgcttc
      781 cgaaattggc atcaagatcg gccggtgttg cttggtaatc gatgccgaag ctgtcccata
      841 aggtatcgat ggcgcgttgc tgcgcggcgg tcatatggct ttggcggagg acgaagctgc
      901 ggatgctgcg ggggtgtccg gcttgacggt cgtttggtgt ttggttttcg gcggtgtctg
      961 tcataatgct tccgtgcgct tgtgcaatca ggatgccgtc tgaatgtgaa aggcctgttc
     1021 ggacggcggg aagggaaagg ggcgattata cctgtttcgt ttcggcaggg taggtgtaac
     1081 gctgttcaga cggcattttg ccattgttgc agcagttctt gcaggacgct tttttgcccg
     1141 tatccgttgt ttatggcttc ttgcaggtag cggcaggctt tttctttgtc cggcccgaca
     1201 ccgtgtccgc tgtaatggat gcaggcgagg ttgtagaacg ccatactgtc tttttttgcg
     1261 gcggcttttt caaaccattt gcgtgcttca ttgtagtcgg cggtcatacc ctgtccgaag
     1321 taatacatgc tgcccagatt ggtttgggct ttgctgtgtc cgagttctgc ggcttctgtg
     1381 tagagtttga gcgcggctgc gtattcgggc gcgcattgta atccgtaatg cagggcttgg
     1441 gctttttgat aaagctgttc tgccctttgc cgcctaatgg cggaatcttt aatgccgccg
     1501 tattggtcag gatggtttaa cgcgctgtcg gatatgagtt tctgataggc ggtaaggctg
     1561 ccggcttcgg ctgcctgccg gtaataatgc cgggcttttt ccgtatcggg ggctatgccc
     1621 aaaccgtagc ggcagatgtc gcccagtatc cgcaaacctt cgggatggcg ttcggcggcg
     1681 gctgcttcgg cgtgatgcag ggcaagtttg tagtttcttt ccggatgctt gccttgcagg
     1741 tagatgtcgg caagtgcggc atgggcatcg gggtcatttt cctgtgcggc ctgttgaaac
     1801 cggtaaacgg ctttatcggg agccgtaggt aggatgagct cggcaagcgg ggtgtaggcg
     1861 ggaatgtatc cttgttgtgc ggcggcgcgc aaatgattga tagcttgggt cgtgtcggtt
     1921 ggtgtacctt gtccgaaacg gtattgaagg ccgagctgcc attgtgccgc cgccaagcct
     1981 tgtgctgcag cctcgcggta gagaaggtgt gccgtttcaa aatccggcgg cgtgccgtac
     2041 cggcagtatt gggcgagcag gtattgcccg tggggattgt tgttttttgt gcttgttttt
     2101 gcccattcca aggcttcttc aaaatgtcgg gacgcataca gcaggtacat caggcggact
     2161 tgtgcttcag gcagtctttc ttctgccaac ggtctgtaaa gcggtatcag ggtttccggg
     2221 ggcgcgcctt ccaattcttg gatatatgcc agattgtaac gcgcataggg atgcccctgt
     2281 tcggcggcga tgcggagcat ttccatacct tgttcgcggt cttggtaggg gctgtccgcg
     2341 tccatcaggt gttttgccaa ctcgaatgcg gcggcgggat gacctgccga ggccgcttct
     2401 gtcagcagtc ggcggacttc ggtaaagtta tattgttttt gagacaggcg ttccaaggcc
     2461 tgccgatagg gtaagtcggg attgttcata tcagatcgtt ttccgttttg tcgaattatc
     2521 gttattcgtt atgaagttag gtttatatcg gcacgccgac ctactgtatc atatttttta
     2581 cagacggttt ttgctttggc ggcatactaa tgcagatgat tgtaaatggt ttccgccaat
     2641 gccttgctga tgccttccac ttgttccaaa tcctcgcggc tggcggcaat cacgccgcgc
     2701 aggccgccga agcgggtgag cagggcttgg cggcgtttgc tgcctacgcc ggggatgtcg
     2761 cttaaggagg aggtaacgcg ggctttgtcg cgttttttgc ggtggccggt aatggcgaaa
     2821 cggtgcgatt catcgcgtac ggtttgcaat aaatgcaagg ccgggctgtg tgggggcagg
     2881 cggaagactt cgccggtaaa aggcagtatg agctcctcca taccggcttt gcgctccggg
     2941 cctttggcaa tgccgaccaa agggatgtgc agcccgagtt cttcccatac cgatacggct
     3001 acgccgattt gccctttgcc gccgtcaatc aacacggcat ccggccattt gacggtctcg
     3061 ccgttggctt cggcttcctg cattttgccg taacggcgcg tcaacacttc gcgcatggcg
     3121 gcgtagtcgt cgccgggttt ggcggtcgtg atgttgtagc ggcggtattg cgagggctgg
     3181 atgttttgct catcgtacac gacgcaggag gcgatggtgg cttcgccttg ggtgtggctg
     3241 atgtcgaagc attcgaggcg gttgaggccg tctgaatcca taccgaggat tttggcgagt
     3301 tcgtcgatgc ggtgttgctg gttgctttgt tgcaggcggc gttgtgcaat cgccatttgt
     3361 gcgttttgtt ccgccatttt caaccaaact ttgcgttcgc ctatggtctt ggtgacaaat
     3421 tgcatttgtt tgccgtgttc gccttccaaa gcctctttca gcgcatcggg aacggaaaag
     3481 ttgctaataa tgatgtcggg tttgcttttg cccaaataat gttgggcgac gaaggcttcg
     3541 gcgtaatctt gtccgtttgg ctcgggatcg tttttggtgt cggggaagaa acttttgtct
     3601 ccgacgtgcc gcccaccgcg tatgctgacc cagtgtacgc agacgatgcc gtctgaaacc
     3661 gccagcgcga gcaaatcgat atcgttgggg ttgttcgggt ttttgctgtc gatgaactgg
     3721 ttgctctgca taatgccgag tgcttggatt tggtcgcggt agcgggcggc ttcttcaaat
     3781 tgcagattcg cggcggcagt ttgcattttg tgttgcaggg tgcgggtcag ttcgtcggtt
     3841 ttgccattga gaaaagtggc ggcttgacgc acgctgtcgc ggtagtcttc ttcgctgatg
     3901 tgtccgacgc agggcgcggt gcagcgtttg atttgataca gcaggcaagg gcggtcacga
     3961 tgctcgaata cgctgtcttc gcaggtacgc agcataaaga ctttttgcaa cacttgaatg
     4021 ctgtcgcgca cggcgttgct gttgggatac gggccgaagt attggtttgg ctttttcagc
     4081 gtgccgcggt aatacgccat ctgcggatat tgatggccgc tgagcatcaa ataaggatag
     4141 cttttgtcgt cgcggaaaag gatattgtat ttcggcgaca aggctttgat gaggttgttt
     4201 tcgagaatca gggcttcggc ttcggaacgc gtgatggtgg tttcgatatg atgaacctgt
     4261 tttaccatca atgcgatgcg cggcgaatgg tcgttttttt ggaaatagcc ggacacgcgc
     4321 cgcttgaggt tgaccgcttt gccgacgtat aagactttgc cgccttcgtc aaaaaaacgg
     4381 tacacgcccg gcagcttggg caggtttttg aggaagagcg ggatatcgaa ttgttccgga
     4441 gaacggtttt ctgtattcat atcaattatt tgaaaaacgg cttcagacgg catatcggca
     4501 gcggcagcct tatccgttcg gaaaagctgt cgggtggtta tatgcggtca atcgattttt
     4561 acccgtttca ggcgcaaggc gttgctcaac acggaaaccg agcttgccgc cattgccgcg
     4621 cctgcgatga cagggtttaa aaaaccgagc gcggcaagcg gaatgcccaa aatattgtag
     4681 aagaaggcga aaaacaggtt ttgcttgatg tttctcaacg tcgcccgcga taccgatagg
     4741 gcatcggcga gttggttgac cgaatgttgc atcagcgtgg cggatgcggt gtgttcggca
     4801 acgtccgcgc cgcctttcat ggcgaagcta acgttggcgg cggcgagcgc aggtgcgtcg
     4861 ttgatgccgt ctccgaccat cgccacggtt ttgccggcgg ctttgagttt ctgcacttcg
     4921 gcggctttgt cgcgcggact catattaccg aaggcgtgtg cgatgcccag ttgtttggcg
     4981 acgtattcga ccgtgccttg gttgtcgccg ctcataatat agacatcgat attgtgtttt
     5041 ttcagacggc ctatggcttc ggcggtatcg gctttcaacg cgtcggcaag tgcgaatgcg
     5101 ccgatgggtt tgttatcgac tgagaccgca acaatgcttg caatatccca aacgccgtct
     5161 aaaaacttcg gcaaggccag ttcggcaaat tcggctttgc ctgctttcac caaacccacg
     5221 ccttccactt cggcggtaat gcctgcgccg acaacggttt gtgcgttttg tgcggcggga
     5281 atgtccaaac cgcgcgcttg ggcggcggag acgatggcac gggcgagcgg atgggcggcg
     5341 ttttgttcga cggcggcggc gatgcggtac aaagcgtctt cgtcaaagcc gctgtcggga
     5401 acgcaataaa cggcggcaac ctgcgggctg ccttcggtca gcgtaccggt tttgtccaac
     5461 acgacggcat cgacgtgggc ggcttcctcc attgctgccg cgtctttaaa ccaaataccg
     5521 tgtttcaccg ctttgccaat gccgaccata atcgcggcgg gtgtagccag accgagcgca
     5581 cacgggcagg caatcaccaa aacggcgacg gcgtgcatca gcgcaaccgt ccaatcgccc
     5641 ttaatcagcc aagtaacaat aaaagtcaac aacgcgatgc ccacgacggc aggcacgaac
     5701 accgccgccg ccttgtccgc cacgcgcgca atcggtgctt tactgccttg cgcttcagag
     5761 agtgcgttca tcatgtcgcc gagcagggtt tggctgccga gctgcgcggc gcggtacacc
     5821 acgctgcctt cagtcatcag cgcgcccgcc aatactttgc cgcctgcctt tttctcttcg
     5881 ggattggatt cgccggtaag atggctttcg tccgcccagc cgctgccgct ttctatgatg
     5941 ccgtcggcgg caatgcgttc gccgtgattg gcgcggatta ggtcgccgat ttgcacttgg
     6001 tcgatgggta gctgccgcca ttcgccatcg cgttgcacgt tgacttgggt tggcgtgagt
     6061 ttgagcagca agcccaagct gttcaggctg gattttttgg tgcggtgttc caaaaattta
     6121 cccagtgaca caaaaccaat caccattatg cctacttcaa aatacacatg cgccataccg
     6181 tacgccgcgt gcgggctgaa aaacagcata tagacggaat acaggtaaat cgagaccgtg
     6241 ccgatggtaa cgagtacgtc catattcgcc agcccgcctt taatgctcgc ccacgcgctt
     6301 ttgtaaaatg gcaccgccag ccaaagctgc accacactcg ccaatgcaaa ctgcaacaag
     6361 ggcgacaaca tccaatcatg ccgtgtccaa ttcagccctt ttagcatcat ccctaccata
     6421 ccgataagga acgggatatt gatggccagc aaaagccaca acctccagcc gatatggtgt
     6481 tctgcttcgg gttgcggcaa tgtatcttcc gtcttttcct tcgcgccgta accggttttc
     6541 tcaatgattt tggcaatgtc ggcagcggaa gttttgctgt cgtcaaacgt aacctgcgcc
     6601 tcctcgctgg cgaagttcac tcccgctgat ttgacaaagt cttttttgtt caacactttt
     6661 tcaatgcgcg aagcacatgc ctgacaggtc atgccttcga tttggaaacg gattttttgt
     6721 tgcataagcg tgattcctgc tttgatattc agacggcagt taaggctgcg ggatgccgtc
     6781 tgagcgccgg tatggtcgaa atttctcaaa cgcgccgata attcgagtag aatccgaaaa
     6841 cttgacacag caaaggaaaa acaatgtccc aacaccgcaa actgattatt ttgggttccg
     6901 gccccgccgg atacaccgcc gccgtctatg ccgcgcgtgc caatttaaac cccgtcatta
     6961 ttacaggtat cgcgcaaggc gggcaactga tgaccaccac cgaagtggac aactggcctg
     7021 ccgatgccga cggcgtgcaa gggccggaat tgatggcgcg gtttctcgcc cacgccgaac
     7081 gtttcggaac ggaaatcatt tttgaccaaa tcaacgccgt cgatctgcaa aaacgcccgt
     7141 ttacactcaa aggcgatatg ggcgagtaca cttgcgatgc cctgattgtc gcaacaggcg
     7201 cgtccgccaa atacctcggt ttgccgagtg aggaagcgtt tgcaggaaaa ggcgtttccg
     7261 cttgtgccac ctgcgacggt ttcttctaca aaaaccaaga tgttgccgta gttggcggcg
     7321 gcaatacggc agttgaagag gcactctacc ttgccaatat cgccaaaacc gttaccctga
     7381 tccaccgccg cagcgagttc cgtgccgaaa aaatcatgat cgacaaactg atgaaacgcg
     7441 tggaagaggg caaaatcatc ctcaagctgg aaagcaacct gcaagaagta ctgggcgacg
     7501 atcggggcgt aaacggcgca ttattaaaaa acaacgacgg ttctgagcaa caaattgccg
     7561 tcagcggcat ttttatcgcc atcgggcaca agccgaatac cgatattttc aaaggtcagt
     7621 tggaaatgga cgaagccggt tacctgaaaa ccaaaggcgg cacggcggac aatgtcggcg
     7681 caaccaatat cgaaggcgta tgggcggcgg gcgacgtaaa agaccatacc taccgtcagg
     7741 cgattaccag cgcggcttcc ggctgtcagg cggctttgga cgcggaacgc tggctgggca
     7801 gccaaaacat ttgaatgccg tctgaaaccg aaattttcgg cggaatgcaa gtttttcgca
     7861 ccaaatcctt gattccgtcg gcaaacccca atataatgcc cgttctttga accttgcctt
     7921 ctgctttcgc atggcagaag gctgttttta ccatccataa ggagataaca tgcgtcatta
     7981 cgagatcgtg tttatcgttc atcctgatca aagcgagcaa gtgcccgcta tggttgaacg
     8041 ttacaaaacc atgattgctg aagcaaacgg taagattcac cgtttggaag attggggtcg
     8101 ccgccaactg gcttacccga ttaacaaaat ccataaagcc cattacgttt tgatgaacat
     8161 cgaaaccact cccgaagtgg ttgaagagct ggaaaccgca ttccgcttca atgatgccgt
     8221 attgcgccat ctaaccatca aaaccaaaca cgccgttacc gaagcatccc ctatgttggg
     8281 tggtgaaaag gctaagaacc tgttgagcgg tgcgtctgaa gaagcggtcg cccaataatt
     8341 gggattcaat aatcttgttt cgcttgccgc gttaattgaa aaggttttcc ctattcgata
     8401 tacgcctgcc ggaatccctg ttttagatat tattttaaag cacaaatcgt ggcaggagga
     8461 aaacgggcag caatgccttg tccaattgga aattccggca cggattttag gcaggcaggc
     8521 ggaagagtgg cagtatcggc aaggtgtata tgttcacgtc gaaggttttt tagctcaaaa
     8581 aagcagacgt tcccttatgc cgatgctcag gatacaaaat attcaagaat ataaaggtta
     8641 aacgacaatg gctcgtcaat cattcaaacg tagaaaattc tgccgtttca cggctgaaaa
     8701 aatccaagaa gtcgattaca aacaagttga tttgctgaaa gactttatct ctgaaaacgg
     8761 taaaatcatt cctgcacgca tcacaggaac gaaggcattc taccaacgcc aattggctgt
     8821 tgccgtaaaa cgcgcacgct tcctggctct gctgccttac accgaccaac acaaataatt
     8881 ttggagattg aatcatgcaa attattctgt tagaaaaaat cggcggtctg ggcaacttgg
     8941 gcgacatcgt aaccgttaaa aacggctacg cccgcaactt tctaattccc gcaggtaagg
     9001 caaaacgtgc gaccgaagcg aatatgaaag agtttgaagc acgccgtgcc gaattggaag
     9061 ccaaacaggc tgaaattttg gcagatgccc gagtccgtca ggaaaaattg gacggtcaaa
     9121 ccgttaccgt tgctcaaaaa gccggtgtgg acggccgcct gttcggttcc gttaccaatg
     9181 ccgacattgc tgctgcaatc gttgctgccg gcatcgaagc cgtgaaagca aatgtacgtc
     9241 tgccgaacgg tcctctgaaa gccgttggcg agtacgaagt ggaagtggct ttgcacactg
     9301 atgccgttgc taaaattacc gttgccgtcg ttgccgcaac cgagtaattg aaattatcag
     9361 atgccgtctg aaaccctgcc gattgtcgga atgtggtttc agacggtatt tgttttgttt
     9421 tccgtatatt ctgcaaaaat cgggatggtt gtttttatag tggattaaat ttaaaccagt
     9481 acagcgttgc ctcgccttgc cttgccttgc cgtgctatct gtattgtctg cggcttcgtc
     9541 gccttgtcct gatttttgtt aatccactat atttgcctgt ggtctttgca ggaatagttt
     9601 gttaacgaaa tttgatgcat aaaatgcgcc aaaaaatatt ggctgtgttt taatatagtg
     9661 ttgataagcg tacctggctt caatatactc aataggatag ctctcttcct aaccgaaaaa
     9721 ataattgtct tttcccttgc aaataattga aatcaacaga ttactaaaac acagccaaaa
     9781 tattcaattg acaaaaactt ttctgatttt gagaaatctg tgaaaatcag gggattattt
     9841 attttgaaaa tggtatcggg cataaaattt ggctgtgttt caatatggtg ctgataacat
     9901 actttgtttc aaaatgttca ataggataac ttcctgattg accgaaaaat aatggccttt
     9961 tcccttgcaa gcagttgaaa tcaacagatt gtcaaaatac atcctaaaaa tttaataact
    10021 tatatatatg attgcaaagg ttgcggcttg tatgccggtc attatggcgg aaataccgtt
    10081 tgggaggcag ccgcatcgcg tggaatcagc acggcagccg catcgcgtgg aatcggcacg
    10141 gcaggcgatg cgccgatgcc ttcgggcgtg aaggcggggc aatcggcagt ttggaaaaag
    10201 gcaatggaat ctgtgggcag aagaagttaa gggtaaaagt cgggcgcacg gtcaggttgt
    10261 ctgtcctttt gtcggaacag gaggcagggc agctttgtcc tgattaattg cttgtgcaaa
    10321 cggtaagaag gtttttaaag caggttgtac agcatttttc cggcaatcaa aagcaacata
    10381 atgccgaagg attttttgag tttggcagaa gaaagtttgt gggcggtttt gacaccgagc
    10441 ggggcaaagg caatagttgc cgcgctgagg acggcgacgg cgggcaggta aaggaagccc
    10501 agtgaccctt cgggcaatcc tgcaatattc aggccgttga gcagatacga tattgcgccg
    10561 gagagtgcaa tcggccaggc aaggccggat gatgtgccga tggctttatg ggcggggaag
    10621 ccgcagtgga ttaagaaggg gacggaaagt gaaccgccgc ctatgccgac ccagctcgac
    10681 attgcgccga acagtgtgga aaccgcagtc agtccgggca gtccgggcag cgggcgggat
    10741 gccgtctgag ggccggtatg cagtgttttg aatgcgacgg cggttaaaaa caggatgaag
    10801 aaaatttgaa gcccgaacgc tgggatatat tttgcggaga gtgcgccagc gaatacgccg
    10861 aataccatac ccggcatcat cgtaaatacg gttttccagt cgaccgcctg ttttttgtgc
    10921 tgccccagca tactggaaaa ggcggtgaag accatgacgg cgaaggatgt gccgacggcg
    10981 aggtgttgcg tgtaaggatg ttgtgccaaa ccctgcaaat caagtaccca taaaacgaca
    11041 gggacaatca gcgtgccgcc gcctacgccg aacagaccgg caataaaacc tgccgcactg
    11101 cctacggcaa gcaggattaa gataatgtcc caatgccaca tttaatccgc ctttctgtat
    11161 tttttccaga atcggcgtgc cgccataatg tagccggaca ggctgtatcc gagaaagaac
    11221 aggaagagga cgagcgacgg ttcccaagtg accagaagca gcaataagac ggcaagcagc
    11281 attccgacaa aggggacttg tctgcggatg ttgatttctt taaaactcca aaaagggatt
    11341 tggacaatca tcgacaggcc ggcaaacagt gtgatgccca atgcccacca gtggacggcg
    11401 gggaattttt cgacgctgtg gttgacccaa atcagcccga caatcagcgc ggcggcagtc
    11461 ggactgggca cgccgataaa ccagcgtttg tccaccttgc cgatgagcgt gttgaacagg
    11521 gcgaggcgca gggcggcgca ggcgcagtag atgaaggcga cggaataacc gattttgccg
    11581 aactgccaaa gctgccattt gtaggcaata agcgcggggg caacgccgaa gctgaccata
    11641 tcggcaaggc tgtcgagctg ttccccgaac gcgctttggc tgttggtcag ccgcgccacg
    11701 cgcccgtcca taccgtccag caacatagag atgaataccg cgatggcggc ggtttcataa
    11761 cgtccgtgca tggattgggt gattgcgtaa aatgcggaaa acagcgcggc gatggtaaag
    11821 gagttgggca gcaggtagat actgttttga cgcagggagc gttgcggggt gttggtcggg
    11881 gtttccatag ctgaagtgtc tcacggacgt ggagaatcga taggggatta taacccaaac
    11941 cattatcgaa caatacgccc tttaggatga agccctcgga ggcggtttgt ttttctgatg
    12001 gcggatgctt tgcggggcgg tttgactctg cctgccaccg ggtgtgttgt ttcggatttt
    12061 tgccggatgg atattgttgc cgggcatttc tgggcttgcc ggaaggtatc ggggggaggg
    12121 catcggaata tgcggcgcac cgttcaaacc ggggcggcgt ttccctattt tcagggggtt
    12181 gcaggtttca acaaattgaa agatattttt tgcatttccg accgtatgct gattttgaag
    12241 cgggataaat tataggacaa ggtttttcgc ccgccgcaaa ggttggccgc aatgccgtct
    12301 gaaaacaacg gcttgccgct aaggcggcgg tatgcggatt tgcgctgccg aaatcggttt
    12361 gccatacccg gcggtgcaat ctgcggggag ggataagccg ataaggcggg acttatcctg
    12421 ttttttgttt ataatgcaac agaatttgga cgaaccgtcg ggaatttatc atgatgaaaa
    12481 agatactggc cgtatcggca ctatgcctga tgactgcggc ggcacgggct gccgatactt
    12541 acggctatct cgccgtttgg cagaatccgc agaatgcgga cgatgttttg caggttaaaa
    12601 ctacaaaaga agattcgacg aaaagcgaag cgtttgccga gttggaagcc ttttgcaaag
    12661 gtcaggacac gcttgcgggc attgccgaag acgagccgac cggatgccgg tcggtcgtgt
    12721 cgctgaacaa tacctgtgtc gcgctggcat acccgaaagc cttgggcgtg atgcgcgttg
    12781 aaaacgccgt cgtgattact tctccgcgtt ttacgagcgt tcatcaggtc gcactcaacc
    12841 agtgcatcaa aaaatacggc gtacagggac aatgcggctt ggaaacagtg tattgcacat
    12901 cttcttctta ttacggggga actgtgcgct ctttgattca aaatctcaaa taaaacggaa
    12961 aatgccgtct gaaagatgtt cagacggcat ttctatatcg acggtcagga ttctttcgga
    13021 tcgggcagca ggctgttcaa cataatggca agtacggcgc acaagcccac gccggcaaag
    13081 ctgaagctgc ccaatttgag cgtcatgccg ccgatgcccg tggtcagtac cgagctgacg
    13141 atgaccaggt ttttcggcag catcaaatcg actttggcat caatcagcgt tttcacgccc
    13201 aaagaagcaa tcgtgccgaa cagcagcagc ataatgccgc ccattactgg catcggaatg
    13261 gaagccaaaa acgcattgaa tttgccgaaa aacgccatgc agacggcaaa aaccgccgcc
    13321 caagtcatga tgacggggtt gctgtttttg gtaatcatca ccgcacccgt tacttcgccg
    13381 taggtcgtaa ccggcgggcc gccgatcaga cccgcaacgc atacgcccaa accgtcgccc
    13441 gtaagggttt tgtccaagcc cgggtctttt gtgtagtctt tccccgttac attgccgatt
    13501 gccatgatgc cgccgatgtg ctcgatggcg ggggcgacgg caacgggcag cataaacagt
    13561 gcagcctgcc agttgatctg aggcgtttca aaatggggaa cggcgaacca gggcgcgtgt
    13621 gcaatgcttg ccgtgtccac cagtcccatc agcagtgcca aaacataacc cgaagcgaca
    13681 ccgatcaaga tgggaatcag cttcatcatc ctgctgccga aaaccgatac gatagcggta
    13741 acggcaaagg taaagccgga aagaatcagc gaatcggtat agtcgatgac ctgtttgccg
    13801 tccgcctgac ccattgccat gctgcttgcc gccacggcaa cagacagacc gatgaccatg
    13861 atgacggggc cgatgacgac cggaggcagc agtttgtgta ccgctgccag tccgcgccaa
    13921 cggatcagcg cggcaaacac aaaatacata aagccggcgg caaacagtcc gaacatggtg
    13981 gaaggcagcc cccattcgcc gacggagtag ataatcggtg cgataaaggc aaacgaagaa
    14041 ccaagaaaaa tcggcacttt gcgtttggtt gtgatttgga acagcagcgt tcccaagcct
    14101 gcgcccaaaa gcgcaagagc cggattcaga ccggtcagca ggggaaccag caccattgcg
    14161 ccgaatgcca caaataaaat ctgcgcgccg gaaacggcaa gtttcagttg gttcatacca
    14221 tcttctttca atcggttcaa acggcacgga ttatactggt ttgccgaggg tctgcgtaaa
    14281 aatacatatg gcgggcaaca gatatgccgt ctgaaaggca atgtctgtct gttgcaggat
    14341 gaaagcagga gaaatggagt ataatccgac actttccata ttcaatccgc aagccgtccg
    14401 gcttccttat ttcagacggc attttccggt aatacggcat gacagaaaaa tcccataaaa
    14461 aaacagcaaa aggcagggcg ggaagcccgt ccccaacgtc cgcacgcaac aagaaagccg
    14521 acaacggcgc acggggcaat aaagtttccg agcggctcaa ggcggtcaaa gagttgcaga
    14581 aaaccgaaac caaaaaggcg cgtcccgaac atgtcgtcaa ccttatcggc gacgcactgt
    14641 ggctgatggg tttggcggca accttgtatt tggcgatttc cctgattagt ttcgatatgg
    14701 gcgatccgtc ttggtcgcac agttcgccgg ttgtggaaga tgtcgccaat tggggcggac
    14761 tgttcggcgc gtatgttgcc gatgtcggct attatctttt cggctggtcg ttctggtggt
    14821 ggatagcggc tgcctgcgtc atgctgtata aaaattttcg tctgcacgca aaacagacgg
    14881 aaaacgaggc atacaaccac aaaatcgctg ccgccgcgct gtttgtcctg acggtcttca
    14941 gccccgtctt ggagtatttt gtgctgggcg gaaaatatgc cgactccctg cctgtcggag
    15001 caggcggtat ggtcggcata cgcgtcggcg cagtgtttgc gtggctgctg gggaaatcgg
    15061 gcagcctgct gattatcttg gttgttctgc tgttgtcgtt gtccctgctg gtgcagattt
    15121 catggctgga atttttgaac ggtgcgggca gggcggttca aaaccgcctg agtgccttat
    15181 ccggcaaggt catggctttg ggaaaacgcc ggccgaatac caaaacagac ggtgtcgata
    15241 cccaaaatac acggcgcatg gtaaaagaag ccaagaatat tacggccaaa cccgttgcct
    15301 tgcccgaagg cagcagcagc aaccgcaaat ccgtcgcggt ttccgtcgcg ccgccgccca
    15361 aaattcaggt ttctctgttt gaagatgacg aacctcggca ggcgggcgaa taccacaagc
    15421 ctacattgaa cctattgcgg attcctgaca gcgaacccgt cagcatcaat cccgccgaat
    15481 tggagcgcac tgccgaactg atcgaatcca aactggcaga attcggcatc ggcgtacaag
    15541 tcgtatccgc cacatccggc cccgtcatca cgcgctacga aatcgaaccc gcgcaaggtg
    15601 ttaaaggcag ccaaattgtt gccttgtcga aagatttggc acgctctatg tcgctgcagt
    15661 ccgtgcgtat cgtcgaaacc atcgcaggta aaaacacgat gggcatcgag ttgcccaacg
    15721 ataaacgcca agacgtgatg ttgagcgaaa tcttgtcctc gcccgtgttt gccggagcca
    15781 aatccaagct gaccgtcgcg ctgggcaaag acattgccgg tacccccgtt gtcggcgact
    15841 tggcgaaaat gccgcacctt ttggtcgccg gtatgactgg ttcgggcaag tccgtcggcg
    15901 tgaacggcat gattatgtct atgcttttca aagctacgcc cgacgaagtc cgcttcatta
    15961 tgatagaccc gaaaatgctc gagttgagca tttacgacgg cattccgcac ctgctctgtc
    16021 ccgtcgtgac cgatatgcgc gaagcagggc aggcgttgaa ctggtgcgtc gccgaaatgg
    16081 aaaaacgcta ccgcctgctt tcccacgccg gtgtacgcaa tctggaaggc ttcaaccaaa
    16141 aagtcgaagc cgcaaaagcg gcaggcaagc cgctgctcaa tccgttcagt ctgaaccccg
    16201 acaaccccga gcctttggaa aaactgccga tgattgtggt cgttatcgac gaacttgccg
    16261 acctgatgat gaccgaacgc aaagccgtcg agcagcaaat cgcccgtctc gcccaaaaag
    16321 cgcgcgccgc cggtatccat atgattgtcg ccacccaacg tcccagtgtc gatgtcgtta
    16381 ccggcctgat taaagccaac atcccgacgc gtatggcgtt taccgtgcaa agcaaaatcg
    16441 acagccgtac catcctcgac caaatgggcg cggacgaact gctcaaatat ggcgattcgc
    16501 tgttcctcca gcccggcagt gccgaaccga ctcgcctgca aggcgcgttt gtttcagacg
    16561 acgaagtaca tcaagtcgtc aactacgtca aatcgcaagc cccagccgac tatattgaag
    16621 gtctgctcag cggcgaggcc gcgctggaaa ctgccaatat cgttaatccg aatgcagaca
    16681 gcgacgaatt gttcgatcag gcagtcgcct atgttttgga aagcaaaaaa acctccattt
    16741 cgtctttgca gcggcagttg cgtatcggct ataaccgcgc ggcaaacctg atggaggcac
    16801 tggaaaatgc gggtgtcgtt tctcccaccg acctcaacgg cagccgtaaa attttggcgc
    16861 acaaggacca tttgtagctc gtattgcaaa atgccgtctg aacggcggaa ttggcgtttc
    16921 agacggcata ttatgtttca ggcgaaacat tgtgatatac ttgccagcta aaatttcccc
    16981 tttgcggcaa tgcggttcaa atatcgtacc gttgcgctgt ttgcttcccc atgtagggaa
    17041 gaaagtttat cattttatca acacaacaaa tttaagggct tatgatgagc gtaactgttg
    17101 aaactttaga aaatctggaa cgcaaagtag tgttgtccct gccttggtcc gaaatcaacg
    17161 cagaaaccga taaaaaactg aaacaaaccc aacgccgtgc aaaaatcgac ggtttccgtc
    17221 cgggtaaagc acctttaaaa atgattgccc aaatgtacgg tgcgagcgca caaaacgacg
    17281 tgatcaacga gctggtgcaa cgccgcttct acgatgttgc cgttgcccaa gagttgaaag
    17341 tggcaggctt cccccgtttt gaaggcgttg aagaacaaga cgataaagag tctttcaaag
    17401 ttgccgccat ttttgaagtg ttccccgaag tcgttatcgg cgatttgtct gcacaagaag
    17461 ttgaaaaagt aaccgcttcc gtcggtgatg ccgaagtgga ccaaaccgta gaaatcctgc
    17521 gcaaacaacg cacccgcttc aaccatgtcg aacgcgaagc ccgaaacggc gaccgcgtca
    17581 tcattgactt tgaaggcaaa atcgacggcg aaccttttgc cggcggcgca tccaaaaact
    17641 acgccttcgt attgggcgca agtcaaatgc tgcctgaatt tgaagccggc gtagtcggca
    17701 tgaaggctgg cgaaagtaaa gacgttaccg tcaatttccc tgaagactac cacggtaaag
    17761 acgttgccgg taaaactgcc gtgttcacca ttacgctgaa caacgtttcc gaagcgactc
    17821 tgcctgaagt cgatgcagat tttgcaaaag ccttgggtat tgcggatggc gacgttgcca
    17881 aaatgcgcga agaagtgcag aaaaacgtaa gccgcgaagt ggaacgccgc gtaaacgaac
    17941 aaaccaaaga atccgtaatg aacgcgctgc tcaaagccgt agagctgaaa gcacctgttg
    18001 ctttggtcaa tgaagaagcc gcacgcttgg caaacgaaat gaaacaaaat tttgttaacc
    18061 aaggtatggc tgatgctgcc aacttggatc tgcctttgga tatgttcaaa gaacaagccg
    18121 aacgccgcgt gtctttgggt ctgattttgg ccaaactggt tgatgaaaac aaactggaac
    18181 cgactgagga gcaaatcaaa gccgttgtcg ccaacttcgc agaaagctac gaagatcctc
    18241 aagaagtaat cgactggtac tacgcagagc cttcccgcct gcaagccccg acttctttgg
    18301 cgatagaaag caacgtcgtt gatttcgttt tgggtaaagc caaagtaaac gaaaaagctt
    18361 tgtcttttga cgaagtgatg ggcgcgcaag cctgattatc ctgaaaatgc cgtcttaggg
    18421 cagtttttga aagcaccgaa gccgctttta aagcccgctt tggtgctttt acatcatgaa
    18481 aggagacgaa atgtcttttg ataactatct tgtccctacc gttatcgagc agagcggtcg
    18541 cggtgagcgt gcattcgata tctattcccg gcttttgaaa gagcgcatcg tattcttggt
    18601 cggcccggta accgacgagt ccgccaatct ggtggttgcc caactgttgt ttttggaaag
    18661 tgagaatccg gataaggata ttttcttcta tattaactcg ccgggcggtt cggtaacggc
    18721 cggtatgtcg atttacgaca ccatgaattt catcaagccc gatgtatcga ctttgtgctt
    18781 ggggcaggca gcaagtatgg gcgcgttctt attgtcagca ggcgaaaaag gcaaacgttt
    18841 cgccctgccc aacagccgga ttatgattca ccagccttta atcagcggtg gtttgggtgg
    18901 tcaggcatcc gatattgaaa ttcacgcacg cgaactgttg aaaatcaaag aaaaacttaa
    18961 ccgcctgatg gcgaaacatt gcggccgcga tttggcagat ttggagcgcg acaccgaccg
    19021 tgataatttc atgtccgccg aagaggctaa agaatatggt ctgattgatc aaatcttgga
    19081 gaaccgcgct tctttgcagc tttaataaga aacctgagag agacaaatgc cgtctgaaca
    19141 tttcagacgg catttatttt atagagactg tatcgggatg acttttcctt tattgcgttt
    19201 agaactgttt tcgccataat tggttttaac atattcctct acaatagcta ggaattcgct
    19261 tgcaatatta tcacctttca gtgttatttt gcgctcccca tctacataaa ccggcgcgac
    19321 gggtgtttcc cccgtaccgg gcaggctgat gccgatgtcg gccaatttgc tttctccggg
    19381 cccattgaca acgcagccca ttacggcaac gttcagggat tcaaccccgg gataaagggt
    19441 acgccatata gacatttttt ggcgcaggta attttgaaca tcttgtgcca gctcttgaaa
    19501 tacggtactg gtggtacgcc cgcagccggg gcaggcggta accatcggcg taaacgaacg
    19561 caatcccata gtctgtaaaa tctcttgccc gacgacgacc tcctgagtac gcgggctgcc
    19621 aggttccgga gtcagtgaaa tgcggatggt gtcgccgatt ccttcttgaa gcaagacgga
    19681 taatgccgcc gttgatgcga caatgccttt gctgcccata ccggcttcgg tcaaacccaa
    19741 atgcagcgga taggcgcaac ggctgcccag ttcgcggtaa acctgaatca aatcctgaac
    19801 cgcgctgact ttgcacgaca ggatgatttt gtcttcgggc agtcccaata gaacggcttt
    19861 ttcggcagat tccaaagcgg agacaatcag tgcttccttc gtcacttctt cgggcggttt
    19921 cggcgcggaa gaagcgaggt tggcatccat catacgtttg gcgaggcttt gatccaaaga
    19981 accccagttt acgccgatgc ggacggcttt atcgttttca gcagcagtcc gaatcataaa
    20041 ggcaaatttt tcatcgcctt ttacgccttt gccgacattg ccgggattga tgcggtattt
    20101 ggacaatgct ttgccgcatt ctggaaattc cgccaacagg cgttcgccgt tgaagtggaa
    20161 atcgccgata agcggtgtgg catagcccat atcgtccaag cggcggcgga tttcggcaac
    20221 tttggacgcg gcttcggggc tgttgacggt aatacgcacc atttcggatc cggcatcgct
    20281 caattcctta atctgcaatg cggtggcttt tgcatcggca gtgtcggtgt tggtcataga
    20341 ttggataacg acgggtgctt ctgaaccgac ggtaatatga tcgatgcgga cttgatgcgt
    20401 cttgcggcgt tggagtgtgt tcatatggtt tgaatctgtt tattgaccgg tgaggacggt
    20461 ttgcaattct tccgagtagg ggaaattcgc ctgcaattgt gcttcatatt cgtatgccgc
    20521 ctgtgcgttg ccgagggctt tggcaatttt ccagcctagc agcaaatcat cggcctgaag
    20581 gacttctacc ctgctttggt attttttaaa gtagtaatcg gcatcgccca actgcccggc
    20641 cagcattttg gtgcgcgcca gttctttaaa tgcgggtggg aactgcggct gggcggcgag
    20701 ggaacgtttc aaataggctt ccgccaatcc gaattgcccc tgttttgcgc tgcatatgcc
    20761 tttattcagg ttggcaatat aaggggtcgg gtaggtgggg tcggccaggg ctttgtcgaa
    20821 atatgccata gattcggcag ggcggttgag cctgccgcac aggaaccagc cgtagttgtt
    20881 gttgatttcg gcactgtcgg gtttgatgga gagggcttgc cggaaacttt cctgcgcctt
    20941 gtcgttaact ttcaggtatt gatagatttc ggcacggacc agccaggcaa gctcgttttt
    21001 agggtctgat ttcaaggcgt cttcaatact tgccgtcgcc tgacggtagt cctgaccgcg
    21061 catatattcc attgccaact gggttttgat attggaaacc tgattggctt tttctgcccg
    21121 cgaggggcgg taggaagtgc tgcacgcgcc caaggcaagg acaagtaata aagagattcg
    21181 tttggatggc ttaaaaggca taattacccc tgttgtccga ttaaaatctg ctgccatttt
    21241 tgttggcggc gcgttttatc ctgaacctgc cccgccaact gtccgcaggc ggcatcgatg
    21301 tcgtcgccgc gcgtttttcg tacggtaacg acaaatcctg cctgctgcaa aatatcgcgg
    21361 aacacacgga tgttctcatt gctggagcgt tcgtatccgg agtttgggaa gggattgaac
    21421 ggaatcagat tgaacttgca gggaacatct gtgaccagtt cgatcagttc gcgcgcatgt
    21481 tgcgccttat cgtttattcc gtccaacatg acgtattcga aagtgatgaa atccctgggt
    21541 gctttgacca gatagcgttg gcatgcggcc atcaattctt tcaagggata ttttttgttc
    21601 aacggtacga tttggttgcg gacttcgtca ttggaagcgt ggagggaaac cgccaaagcc
    21661 accggcatga catcgcgcaa cctgtccatt tggggaatca tacccgaagt ggaaacggtt
    21721 acgcggcggc ggctcaaacc gtagccgtgg tcgtccagca tgatgcttaa ggcggtaacg
    21781 acattgtcga agttcgccat cggctcgccc atgcccatca tgacgacgtt ggaaatcacg
    21841 cgctcgtttt tcggtgtaac gcccatcgct ttgtttgccc accacaattg cccgatgatt
    21901 tcggcagcag tcaaattgcg gttgaagccc tgccggccgg tcgaacaaaa tgtacattcc
    21961 aaagcgcagc cgacttgtga ggaaatgcag agcgtgccgc gatccgattc ggggatgaag
    22021 acggtttcca ccccgttgcc cgtaccgaca tccaaaagcc attttcgagt gccgtctgaa
    22081 gatttttgag acatcatcag cttgggaatt tcgatgcctg cctgttcgtt cagtttatgg
    22141 cgcaacgatt ttgccaaatc ggtcatttcg ttaaaatttt gcgcgccgga ttggtgcatc
    22201 caacgcataa cctgtttggc acggaaaggt ttttcgccca tatcggcaaa atgtcgggtc
    22261 agcccttgaa ggtcgtagtt gagcagattg gttttcatgt gtttgtgttt ttcttaaatc
    22321 aaggcttcaa ataaaaaatt gcagggcagg caagtttgat ttgaagcctt ttgcaacaga
    22381 gttttcagac ggcatggata tattttatgc cgtctgaaga ggagttgtgc ggtgtattgt
    22441 atcaacgggg gcagatttcg gtttggctga aaaagtaggc aatttccaaa gcggcatttt
    22501 ccggagtgtc cgagccgtgt acggcattga tgctgaccga agtggcaaag tccgcgcgta
    22561 tcgtgccttc ggcggcttcg gcgggattgg tcgcccccat cagttcgcgg tttttcagaa
    22621 cggcgttttc gccttccagt acttgaatca taaccggacc gccggtcata aattcaacca
    22681 atccggcgta gaaggggcgg tctttatgaa ccgcataaaa ttcttgcgcc tctttgaggg
    22741 taagctgctt cattttggcg gcaacgattt tcagaccgtt ctcctcaaag cggctgtata
    22801 ttttgccgat gacgtttttg ccgacggcat cgggtttgat gatggagatg gtacgttcaa
    22861 tcgccatgct atatccttat ttttacttaa gaagaatcaa atcggtattc tatcaaaaaa
    22921 ataacttatc cgcctgaatc cttgggagat agggtcaggt gcggctgttc caaataaact
    22981 tcggactgca tttcgaccat ccggctggcg gtgcgggtaa attcttcggc aaaatcgcct
    23041 tccgtataga tatggttgac atctaccgcg ccggtggcac acagtttgac ccggaaatcg
    23101 tagagtacgt caatcagcca agtcagccgc cgcgcctccg ccttttcttg cggtgagagt
    23161 tgttccaaac ctgaaataaa aaccatttca taatgttcgg ccaaatacag atagtcggac
    23221 tgtgagcggg ggccgaagca cagtgcgcgg aaatcaaacc atatggcacg gccggactcg
    23281 gctttgtggg gaatctcccg accgtggatg gtgctgatgc cggggttcaa atcggtaatg
    23341 cctgtcattt ctttgaacag ttttgccagt tttgcctcat tttcttcatt ggcaggcgta
    23401 aagaaaatct cggcggggcg gagggtacgc agtcggtagt cttcaccgcc gtcaacgttt
    23461 aagacggtca ggctggactc gatgagcgcg attgtgggaa gaaaactgct ccggttttga
    23521 ccttgcgggt agagttcgga aggcgcgtag tttgaagtcg ccaccaaaac aacgccctcg
    23581 ttaagcaggt tttccagcag acggcctaaa atcattgcat ccgcaatatc gctgacatga
    23641 aattcgtcaa aacataatac gcgggtttct ttggcaatct cggcggcaac ggatttcaac
    23701 gggttgcttt cgcttttcag ggttttcagc ctctggtgga tttctgccat aaaggcatga
    23761 aagtggacgc ggcgtttgcg gcggtaaggg aggcagccga aaaaagcgtc catcagaaag
    23821 cttttgccgc gtccgacccc gccatagaaa taaagccctt tggggacttg cggggaacgc
    23881 aaactcctgc ctaaaaaacg gtttcttttg cgtttgaaca tcatcaattc ggtccaaagc
    23941 cgatcgaggt gttcgatggc ggctgcctgc gcgtcgtcgc ggatgaagtt gggcagttgt
    24001 gaggcagcct gataccaggt cagcgggctg tggttttcaa acggcggggc tttaaaaagt
    24061 tggtctctat tactcatatt taaccttgtt atacttcttg cggcggagaa agaaaatgct
    24121 taccgtcggt attataaatg atttccttca aacttggcgt tttccgtgga aaagaataat
    24181 gacgcgcaca aggggggtat gagcaaaggc ggcgtttcaa tatggtgcag atatgagctc
    24241 aaatgccttt tatagt
//
LOCUS       DAWKQJ010000010        23711 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000010 DAWKQJ010000000
VERSION     DAWKQJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.010"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(65..904)
                     /locus_tag="WJL20_000314"
     CDS             complement(65..904)
                     /locus_tag="WJL20_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7682514.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WJL20_000315"
     tRNA            1189..1264
                     /locus_tag="WJL20_000315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WJL20_000316"
     CDS             complement(1329..2522)
                     /locus_tag="WJL20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7682515.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WJL20_000317"
     CDS             complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WJL20_000317"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ7682516.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3774..4874
                     /gene="aroC"
                     /locus_tag="WJL20_000318"
     CDS             3774..4874
                     /gene="aroC"
                     /locus_tag="WJL20_000318"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ7682517.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGCEHISQNPFFAANQSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4966..5370
                     /locus_tag="WJL20_000319"
     CDS             4966..5370
                     /locus_tag="WJL20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ7682518.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLITALPQYDPTLIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(5444..7429)
                     /gene="parE"
                     /locus_tag="WJL20_000320"
     CDS             complement(5444..7429)
                     /gene="parE"
                     /locus_tag="WJL20_000320"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ7682519.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7496..8020)
                     /locus_tag="WJL20_000321"
     CDS             complement(7496..8020)
                     /locus_tag="WJL20_000321"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ7682520.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8186..9481
                     /gene="serS"
                     /locus_tag="WJL20_000322"
     CDS             8186..9481
                     /gene="serS"
                     /locus_tag="WJL20_000322"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ7682521.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9554..10552)
                     /locus_tag="WJL20_000323"
     CDS             complement(9554..10552)
                     /locus_tag="WJL20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7682522.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10677..11753)
                     /gene="prfA"
                     /locus_tag="WJL20_000324"
     CDS             complement(10677..11753)
                     /gene="prfA"
                     /locus_tag="WJL20_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ7682523.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDTDDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11843..12613)
                     /locus_tag="WJL20_000325"
     CDS             complement(11843..12613)
                     /locus_tag="WJL20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7682524.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12728..13717)
                     /locus_tag="WJL20_000326"
     CDS             complement(12728..13717)
                     /locus_tag="WJL20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ7682525.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIATALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13784..14467)
                     /locus_tag="WJL20_000327"
     CDS             complement(13784..14467)
                     /locus_tag="WJL20_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7682526.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14656..15990)
                     /gene="glmM"
                     /locus_tag="WJL20_000328"
     CDS             complement(14656..15990)
                     /gene="glmM"
                     /locus_tag="WJL20_000328"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ7682527.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16120..16971)
                     /gene="folP"
                     /locus_tag="WJL20_000329"
     CDS             complement(16120..16971)
                     /gene="folP"
                     /locus_tag="WJL20_000329"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ7682528.1"
                     /translation="MARHGWQAGRFEIGLDKPKIMGIVNLTPDSLSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVAPVLAEVAGWGVPVSLDTRH
                     TAVMEKALALGGIDIINDVAALTDEGALELLARQADTGICLMHMQGLPKNMQINPKYQ
                     DVVGEVTRYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGELTGETDAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17111..18004)
                     /locus_tag="WJL20_000330"
     CDS             complement(17111..18004)
                     /locus_tag="WJL20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ7682529.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18095..20209
                     /locus_tag="WJL20_000331"
     CDS             18095..20209
                     /locus_tag="WJL20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ7682530.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGTPAIRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20274..21752)
                     /gene="ubiD"
                     /locus_tag="WJL20_000332"
     CDS             complement(20274..21752)
                     /gene="ubiD"
                     /locus_tag="WJL20_000332"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ7682531.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21772..22287)
                     /locus_tag="WJL20_000333"
     CDS             complement(21772..22287)
                     /locus_tag="WJL20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ7682532.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22294..22794)
                     /locus_tag="WJL20_000334"
     CDS             complement(22294..22794)
                     /locus_tag="WJL20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682533.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            complement(23026..23595)
                     /locus_tag="WJL20_000335"
                     /pseudo
     CDS             complement(23026..23595)
                     /locus_tag="WJL20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23635..>23711
                     /locus_tag="WJL20_000336"
     CDS             23635..>23711
                     /locus_tag="WJL20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7682534.1"
                     /translation="MLPSKYEDNTLQMKEKSTERTAPFFV"
BASE COUNT         5364 a         6783 c         6217 g         5347 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa acctgcattg ccgcctttgg caggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaata atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaacctta cgttttacgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagaggccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatc ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacag tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctca ggccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaagccttg gtaggcaatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgctgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccaac agtggcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcctt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg caggaagtcc gccccgactt tgagtgcgcc cgagccgccc
     2221 aaggtttgta cggtagcgat gcgtccttgc gcgagcgcag ggttgtcttt gccgaacaat
     2281 aaatgctgca ccgcgctgcg gtaagtgtcc aagccttcca tcggcaggta gggcgacggc
     2341 gcaggcgtgg cggcgcgggc ggtttcggca cggcgcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcagggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcggg tcgcccggat agtattcgat gtgtcggtac
     2521 atagtcctta ccttttactt tttcaaagga ttttcttttt caacaataca ccactttcga
     2581 tatggtgcgt aaacgggaat tggtcgaaca gggcggcgcg ttcgaccgca tgggtttcca
     2641 ccaaggtgtc caaattggca cgcagcgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gatggaggtt ttggagattt
     2941 cggttgccaa aacacgttca aacttttcag ataaaggaag cgtaaagttg ccgtttccgc
     3001 aatacagttc gagcaaatcc ccatccaatc cttctgccgc accgcaggcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccgatg acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc attatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtagacaa aaattcgacg gcataccaac ggtttctcag ttcggcagat tgggcggcaa
     3361 cggcaatcaa ttctggcatc aggcggttga cggcttcgga agccgcttca aaacggtcgc
     3421 agcgtatcat gcttgcgccg ctggctttct gccctttttc aaacatggca taaaacattt
     3481 ccccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tggtcttttt
     3601 tatcttgaag ctgctgcgtg tagtcgctca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat tcactgcaaa acacgcaacc cctttaccgg catataaccc gttttcctcc
     3721 aacttttatg ctaaaatccg ccgtctccaa cataccgccg acagaaggaa aatatggcag
     3781 gcaacacttt cggacaactc ttcaccgtga ccacgttcgg cgaaagccac ggcgcaggtt
     3841 tgggctgcat catcgacggc tgcccgcccg gactggcatt aaccgaatca gacatccaag
     3901 ccgacctcga ccggcgcaaa cccggcacca gccgccacgt tacccaacgc cgcgaagccg
     3961 accaagtcga aatcctctcc ggcgtcttcg aaggcaaaac cactggcacg cccatcgccc
     4021 tcttaatccg caataccgac caacgcagca aagactatgg caacatcgcc caaagtttcc
     4081 gccccggcca cgccgactat acctactggc acaaatacgg cacgcgcgac taccggggcg
     4141 gcggtaggag ttccgcccgc gaaaccgcag cccgcgttgc cgccggagcc gttgccaaaa
     4201 aatggttgaa agaaaaattc ggcacggaaa tcaccgccta cgttacccaa gtcggcgaaa
     4261 aaaaaatccg gtttgaaggc tgcgaacaca tttcccaaaa tcccttcttc gccgccaacc
     4321 aaagccaaat tgccgagctg gaaaactata tggacagcgt gcgcaaatcg ttggattctg
     4381 tcggcgcgaa gctgcatatt gaagccgcca acgtccccgt cggcttgggc gaacccgttt
     4441 tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg catcaacgcc gtcaaaggcg
     4501 tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg cagcgaacac ggcgacgaac
     4561 tcaccccgca aggcttcctg tccaaccact caggcggcat cctcggcggc atcagcaccg
     4621 gacaagacat ccacgtcaat atcgccatca aacccaccag ctccatcgcc accccgcgcc
     4681 gcagtatcga catcaacggc aaccccatcg aactcgccac gcacggcagg cacgacccct
     4741 gcgtcggctt gcgcgccgcg ccgattgccg aagctatgct cgccttggtt ttaatcgacc
     4801 acgccctgcg ccatcgtgcg caaaatgccg acgttcaggt taatacgccc gacattaccc
     4861 tttcaaacaa ataaaaattt agccaaaaca cagactttat aatagaatac caagcattgc
     4921 cgtgcagcct tgacgcacgg gtttgtttag aggaatacaa ccgaaatgac acaagaaacc
     4981 gctttgggcg cggcactgaa atccgccgtc caaactatga gcaaaaagaa acagaccgaa
     5041 atgatcgccg accatatcta cggcaaatac gatgtattca aacgatttaa accgttggca
     5101 ctcggtatcg accaagactt gattaccgcg ctgccccaat acgatcctac acttattgca
     5161 cgcgttctcg ccaaccactg ccgccgtccg cgctatctga aagccttggc gcgcggaggc
     5221 aaacgtttcg atttgaacaa ccgtttcaaa ggcgaagtta cccccgaaga acaggcgatc
     5281 gcgcaaaacc atcctttcgt gcagcaggct ttgcaacagc agtccgccca agctgccgcc
     5341 gaagcagccg gatcttccgc agcagaataa atccccaaac gaaatgccgt ctgaaaaccg
     5401 atttggtttc agacggcatt ttttcgtatg cggcaatcac ggttcaaata tcgagttccg
     5461 ccgtatcgcc ttcgcgctcc atccaggcac ggcgggcggc ggcttcgcct ttgcccatca
     5521 gtttgacgaa gatgtcgcgc gtctcgtcat ccgcgccttc ggggatttgt acctgcaaca
     5581 ggcggcgggt gtcggggtgc atggtggtgt ctttgagttg gtcggggttc atctcgccca
     5641 agcctttaaa acggctgatg gaataagcag tttctttaaa gccttctttt tgtagtcgct
     5701 ccaaaatgct gtcgagttcg ttttggtcga gggcgtagaa tttgcgggcc ggtttgtttt
     5761 tgccttgtgc attgacatcg acgcggaaca gcggcggctg ggcgacgtag atgtgtccat
     5821 cggcgaccag tttcgggaag tggcggtaga acaaggtcag cagcaaaact tgaatatgcg
     5881 agccgtccac gtcggcatcg gacaggatgg cgattttgcc gtagcgcagg ccgcttaaat
     5941 cgggatggtc gttaataccg tgcggatcga cgccgatggc gacggaaatg tcgtggattt
     6001 cggcgttgcc gaagagttgg tcggggtgga cttcaaagct gttgagcact ttgccgcgca
     6061 ggggcaggat ggcttgggtg gctttgtcgc gggcgagttt ggctgagccg ccggcggaat
     6121 cgccttcgac gaggaagagt tcgttttcgc ggatgtcttc gctttcgcag tcggtcagtt
     6181 tgccgggcag gacggcgacg ccgctgcctt ttttcttttc gattttctta accgaacgca
     6241 tccgcgcctg cgcctgacgg atggcgagtt cggcgatttt tttaccgaag tccacgtttt
     6301 ggttcagcca caattccaaa gggtcgcccg atacggcggc aacgagtttc aacgcgtcgc
     6361 ggttggtcag cttgtctttg gtttgacctt ggaactgcgg gtcgaggacg cgggcggaga
     6421 ggacgaaggc ggttttaccg aacacgtcgt cactttgtac tttcacgccg cgcggtagga
     6481 ggttgtgcag attgatgaaa ttgttgacgg cgttgaacac ggcttgtttc aaacctgctt
     6541 cgtgcgtgcc gcccagcggg gtggggatga ggttgacgta gctttcgttg gcgcacgagc
     6601 cttcttccag ccaggtcagg gcaaacgccg ctccttcgcc gatgctgaaa tcgccgttgt
     6661 ggtcgtctga aatgtagttt tcgcaagaga agataggcac ggcttcctgc gcgtcggcaa
     6721 tcaggtcggt cagatagctt ttcagaccgt cggggtaatg ccaggtttgg gtgtgcgctt
     6781 cgtcttcgcc tttgaccgga cgggtcaggg aaacgcgcac gcccggcagc agcacggctt
     6841 tggcgcgcag caggcgttcg agttcgggaa tgctgtaatt cgggctttca aaatatttgc
     6901 cgtccggcca cacgcgcact tcggtgccgc tgtctttgac ggcgcatttg cccacttgtg
     6961 ccaacggttc gaccacgtcg ccgccggcaa acacgatacg gtggattttg ccttcgcgtt
     7021 tgaccgttac ctcaaggcgg gtggaaaggg cgttggtcac ggatacgccc acgccgtgca
     7081 ggccgcctga aaaggcatac gcgctgccgc cgtctttttt gttgaacttg ccgcctgcgt
     7141 gcagacgggt gaacacgagt tcgactacgg gtacgccttc ttcagggtgc agaccgacgg
     7201 gaataccgcg gccgttgtcg cgtacggaaa gcgaaccgtc ttcatgaatt tgcacgtcga
     7261 ttgccgtagc gaaaccgccc aacgcctcgt ccgccgcgtt atcaatcact tcctgacaga
     7321 tatgggtcgg gctgtcggtg cgggtgtaca taccgggacg ttctttgacc ggttccaaac
     7381 ctttgaggac ggtgatgctg gattcgctgt attggttgtt tttagccata ggaataatct
     7441 gaaagtaaga aaaacaacgc tttcagacgg cctgaaagcg ttgcgttccg ttgttttacc
     7501 ggttgccgga aggttggcgg gcggcaaagt cttcataact ttccataccg cgcaggaagc
     7561 gggaagagag ttctttcaac gcccccaaat aaacgtcgcg tttgaactcg atgacttcgt
     7621 caacgggcgc ccaatattga tgccaacgcc agccgtcaaa ttcggggtgg cgggtggcgc
     7681 gcaggttgac atcgcaatcc cggccggtca ggcgcaggag ataccaaatc tgcttctgcc
     7741 cgcgataaga gccgcgccat tcgcggcgca cccagttgtt cggcacgtcg taacgcagcc
     7801 agtcgcgcgt ccgcccgacg attttgacgt gttgcggcag aagtccgact tcttcgtaaa
     7861 gctcgcggta catcgcggtt tcggggcttt cgcccggctt gatgccgcct tgcggaaact
     7921 gccaagaatg ttcgcgcacg cgcttacccc aaaagacttc gttacgttcg ttaatcagga
     7981 taataccgac attggggcga tagccttccc tgtccaacac ggtgtcgccc tccgttaaat
     8041 tcaatcgtac gattttccca caaatcgggc ggttttgaca aatcagacgg catggcggta
     8101 cgcgtaccga aacacggggg gatttgggaa aatatcttga atttggttta caataatatg
     8161 tttcaaatta ttcgggaatc agaaaatgtt agacatccaa ttgctccgca gcaacaccgc
     8221 cgctgtggcg gaacgcttgg cggcgcgcgg ttacgagttt gatgccgcgc gtttcaatgc
     8281 gctggaagaa caacgcaaag ccgtccaagt gaaaaccgaa gaactacaag cctcgcgtaa
     8341 cagcatttct aaacaaatcg gcgcactgaa aggtcagggc aaacacgaag aggcgcaggc
     8401 ggccatggat caagtcgccc aaatcaaaac cgatttggaa caggctgccg ccgatttgga
     8461 tgccgttcaa aaagaattgg acgcatggtt gttgagcata cctaacctgc cgcacgaaag
     8521 cgtacctgcc ggtaaagacg aaaccgaaaa cgtcgaagtc cgcaaagtcg gcaccccgcg
     8581 cgaatttgac tttgaaatca aagaccatgt cgatttgggc gaacctttgg gtttggattt
     8641 tgaaggcggt gcaaaactct ccggcgcacg atttaccgtg atgcgcggac aaatcgcccg
     8701 tctgcaccgc gccttggcac agttcatgct ggatacgcac acgctgcaac acggctacac
     8761 cgagcattac acgccttata tcgttgacga tacgacgctg caaggtacgg gccaactacc
     8821 aaaatttgcg gaagatctgt tccacgttac ccgtggcggc gacgaaacca aaaccaccca
     8881 atacctgatt ccgacagccg aagttaccct gaccaatacc gttgccgaca gcattatccc
     8941 gtccgaacaa ctgccgctga agctgaccgc gcattcgccc tgtttccgca gcgaggcggg
     9001 ttcgtacggc aaagacacgc gcggtctgat tcgccagcac cagttcgaca aagtggaaat
     9061 ggttcaaatc gttcatcccg aaaaatcata cgaaacgctg gaagaaatgg tcggccatgc
     9121 cgaaaacatc ctgaaggctt tggaactgcc ctaccgcgtg attaccctgt gtaccggcga
     9181 catgggcttc ggcgcggcaa aaacgtatga cttggaagtt tgggtgccgg cgcaaaatac
     9241 ctaccgcgaa atctcaagct gctccaactg cgaagatttc caagcccgcc gcctgaaggc
     9301 gcgtttcaaa gacgaaaacg gcaaaaaccg cttggtacat actttgaacg gctccggttt
     9361 ggcagtcggc agaaccttgg tcgccgtgtt ggaaaaccat caaaacgccg acggcagcat
     9421 caacatccct gccgcactgc aaccgtatat gggcggcgtt gccaagttgg aagtgaaata
     9481 agccttaacc ctgaacataa atgccgtctg aaacttggtt cagacggcat ttcctttaaa
     9541 cttttaaaac acgtcagccg tcggcacgaa ccgcattgcc gcaatcgccg gtctgtccga
     9601 cctcgcggat attggacagc gtaacttccg aaatattacc caacgcctct tccgtcaaaa
     9661 atgcctgatg gccggtaaac agcacattat gacaagacga caggcggcgg aacacgtcgt
     9721 cggtaatcac atcgttggat ttgtcttcaa aaaacagctc gcgctcgttc tcgtacacat
     9781 ccatgcccaa tgcgccgatt ttccggcgtt tcaacgcctc aatcgcggcg gcactgtcaa
     9841 tcagcccgcc ccggctggtg ttgataatca tcacgccgtc tttcattttg tcgaacgccg
     9901 cttcgttcag catatagtgg ttttccggcg tggaggggca atgcagcgtg atgatgtccg
     9961 actgggcata cagctcgtcc aaatccacat attttccgcc gagtttttcc acttcggggt
    10021 tgcaaaacgg atcgtaagcc agcaggttca taccgaaacc ctttaaaatc cgcatcgttg
    10081 cgataccgat tttccccgtg ccgataacgc ccgccgtttt gccgtacata ttgaaaccgg
    10141 tcagaccttc cagcgaaaaa ttcgcatcgc gggtacgctg ataggctttg tggatacggc
    10201 ggttcaacgt cagcatcaga ccgaccgtat gttccgcaac cgattcgggc gaataggcag
    10261 gcacgcgcac gactttcaag cccaactctt cagccgcctt taaatccaca ttattgaagc
    10321 cggcacaacg caacgccaca gttttcacgc caatttgcgc caatttttcc aacacgggcc
    10381 ggctgccgtc gtcgttcaca aaaatacaga ccgcctccgc accttccgcc attttcgccg
    10441 ttttcgcatc cagcataaaa tcaaaaaact ccagctcgaa gccgaaatgc cggttggcgc
    10501 gggtaaaatg ttcgcggtca tagcttttcg taccgtaaat cgcaatcttc atcaatatgt
    10561 ccagttgtcg tctatggttg agaaacggca taatacactg aattcaaaca aatcagtaga
    10621 atatggtgga ttaaaattga ttgcatgcac ggcatttcca tttcaaaaca caaaactcaa
    10681 tcgcccattg ccgccagaag ctccgcctga tgctcggcaa tcagggcatc ggtgatttct
    10741 gccaaatcgc cgtccatcac aaaatccagc ttgtgcaggg tgaggttgat gcggtggtct
    10801 gttaccctgc cttggggata gttgtaggtg cggatgcgtt cgctgcggtc tccgctgccg
    10861 atgagggatt tgcgttcggc ggcttctttg gcttgggctt cgcgtttttg cgcgtcgttc
    10921 aggcgggcgg cgaggacttt catcgcctgc gctttgttgg cgtgttggct gcgtccgtct
    10981 tggcattcga ccaccatgcc tgtgggcagg tgggtgatgc ggacggcgga atcggtttta
    11041 ttgatgtgct gcccgcccgc gccggatgcg cgaaaggtgt cgatgcgaag gtcgacgggg
    11101 ttcaactcga tgtcttccag ttcgtccgct tcgggcatga cggcaacggt gcaggcggag
    11161 gtgtggatgc ggccttggct ttcggtggcg gggacgcgct gcacgcggtg tccgcccgat
    11221 tcaaatttca aacggctgta cgcgcctaag ccgataatac gggcgataac ttctttatag
    11281 ccgcccaact cgctttcgtt ggcggacacg atttcaacct gccagcggtt gcgttcggcg
    11341 tagcggctgt acatacgcag caaatcgccg gcaaacagcg cggcttcgtc gccgcccgtt
    11401 ccggcgcgta tttcgatgaa gatgtttttg tcgtcgtcgg tatctttggg cagcagcagt
    11461 ttttgcaatt cggtatcgag tgcgccgatt ttggctttcg ccgcttcgat ttcttcggcg
    11521 gcaaagtctt tcatttcggg gtcggacaac atttcttcgg catccgccaa atcgctttgc
    11581 gccagttgat agttttggaa tacttcgacg acgggggtca gttcggcgtg ttcgcgcgtg
    11641 agcttgcggt agttgtccat atctgccgtg gcttcgggtt gccctaaaag gtgggtaact
    11701 tcttccaacc ggtcgccgag ttgttgcagt ttttctaaga tagacggctt cataattctt
    11761 ccataacaaa cgccgcctga atttcagacg gcatcaaaac gcggtcatta taatatattt
    11821 tctgaatatt caaaaagata atctagggac ggagcaggct gtcggcattg cgttccgtta
    11881 tcgcggcgat gtccgccacg ccgacaccgc gtatctgcgc ggcaatttcg gcaatccaaa
    11941 gaatattggc aggcgtatta atctcttttt tcagcataaa cgggctgtcg gtttccaata
    12001 cgaaatcccc gtcgttcaag gctttaagcg tatcgcgcac tttacgcgcg ttcggattga
    12061 gcaacaacga accgatgccg attttgaaac ccagtttcgt caacacgcgc gcttcttccg
    12121 cgctgcctga gaaggcgtga acgatgccgc cttgggcaaa gcctgtctgt ttgacggcgg
    12181 cggcgatgtc ggcggtggct ttgagattat ggataatcac gcggcggcgc agggtttgcg
    12241 cgatggcaag ctggcggacg aaaacttgga tttgccgttc gcgctgcggc ggcgtttggg
    12301 ttttgtcgta aaaatccaag ccgatttcgc cgacccatgc ctgcggataa cgtgccaaca
    12361 tcgcttccag gcggacgcaa tcacgctcgg caatgccgtc tgaaaaccaa ggatggatgc
    12421 cgagcgcaat acggatttga ccgtgttcgg acggcatttc cgacagttcc gccacgcttt
    12481 gccaatcctg cggacaggtg gcgggaacga taaaccgctt caccccaact ttccgcgcgg
    12541 cggtcaggat gtgcggcagg ttttcgcgca gggcggcatc ggcaaggtgg cagtgggtgt
    12601 cggtgaagtt catttcgatt ccgcaatgcg gcaggttcac ataaaaataa ggcagggcag
    12661 caagggatgc cgtgtacggg cggcgcataa ggcgcggtca acgccccgtc attgccattt
    12721 caatccgcta taacttcagt cttgccaact ctttttccac ctcttcctta ccccagcctt
    12781 gcgatacggc gagggtcatg agtgcggtgg cggtttcgag gttgcatttg ccgccgttga
    12841 tgatgcctga gttgcggaac gcgttgcctt gcgcgtaaac ggcagcggtt ctgccttgtc
    12901 ggacttggct gatgttgagc agcagcctgc cttgctgcgt aaagtcttgc acggcgcgga
    12961 tgaaaccttc gtctgcgggc gtgttgccgt gtccgtaact ttgcaggata agggcgtggg
    13021 cgggaagctg tccgagtccg tcggcaagtt cttggacggc aaagccggga ataagcgtgc
    13081 ggacggcgat ttttgcctgc gggtcaggat agcggatttt gaggccgtct gaaacggccg
    13141 ccgcatcttg ggacggaata cgaagattgt gccagccctg ggtttcgtcc cactccgcca
    13201 gtgcgccgaa atgcggattg tcgaaaccgg cggcggtttc ggtgctgact ttgctgctgc
    13261 cgacggcggg ataaagtttg ccgtcaaagg caataagcgt ctgcttcaat ccgagtggga
    13321 atgcggcaac ggcagtcgcc agattgcgcg gcgcgtcgct gcccacggcg ttgtacggcc
    13381 attgggagcc ggtcaggacg attggcttgt ccaaaccctg cagcgcgagg gcgaacagat
    13441 tggcggtata ggcgagcgtg tccgtgccgt gcagcaccaa aacgccgtcg tactgcggca
    13501 gcgcggtggc aatgatgtcc agccaagcgc accagttttg cagttggacg gcggaagaat
    13561 caatcagcgt cccacaaacg tgccaatcga aatccatgcc gtttgaaaac ggcgcgagtg
    13621 cctgcccgac cagcgcggta tcggggcgca ggccttcgct gctttgcgtc atgccgatgg
    13681 tgccgccggt ataaaggaca aaaattttct gtttcatggc tcatcctgaa aatgcaaaga
    13741 ccgcctgaaa cgcgaaatgc gtccggacgg tcgaaaatct gatttactgc tgcttctgtg
    13801 ccgctttttt cgctgccttt tccgccttgc gtttggcgcg tttttcgctc aattgtgcgc
    13861 ggtaaagctg ataatgtcgg cgtttgcgcc accagaacca cgccagcgcc gccgccagca
    13921 cgcccaatgc gatgaagatg ccggattgca ggctgtgcat tttcgccatc agccaatcga
    13981 tgttgtgcgc gccgtactcg cccaagtaaa tccaaacggg cacggaaatc agcgcggcaa
    14041 gcccgtccat aatcagaaag cgcagatacg atactttgcg gctgatgccg gcggtaacga
    14101 aaacggcagt ccgcaaaccg ggcaggaaac gagcgacaaa taacacccag ttgccgtatt
    14161 tgtcgaattt ttcctgaacc tgtgcgtaac gtttcggcgt catgatgcgc gcaatcggtt
    14221 tgaacttgag gattttctgc ccccagatgc gtccggcggc gaacatgatg ccgtccccga
    14281 ccaatacgcc gagcataccg actgcaaaca taatatgcgg attggtataa cccatacccg
    14341 aaatcacgcc gcctgttacc aaggtcaaat cctcggggat cggcacgccg aaaccgcaga
    14401 tgaccaaaac gaaaaacacg gccgcatagc cgtattcgac aaaaaaggct tccaaaaggg
    14461 caaacatagc ggatattcca ttgtcggaga taaaaagtca gaacaagccg aaacattttc
    14521 tgcgcgaaac ggcattctat caaagattat gccgtctgaa agcggcaagg ggcagattcg
    14581 cgccctctgc cggacgttca gacggcattt aaacaggaaa accgccgctg ttgaaacggt
    14641 tttgcctgat tttgcctaaa ggctgccgat ggcggaagca atacgttccg caccgtcgcg
    14701 cgcccaatcc gcctgccgcg cctccaccat cacacgcacg acgggttcgg ttcccgaagc
    14761 gcgcaacacg acacgccctt tgccttcgag ttctttttcc acttccgcca acacgtcttt
    14821 tgaggcttcc tgccatttct gacctttttg gatgcgcacg ttaatcatag tttgcggata
    14881 cggctgccaa tcggcgcaaa cggtggcgag gtcttggttc agcgtttgca gtgccgccaa
    14941 aacttgcagc gcggaaataa tgccgtcgcc ggtgttgtgt ttgtccatgc acaaaatatg
    15001 gccgctggct tcgccgccga tgagccagcc gcgttggttc agctgttcca acacatagcg
    15061 gtcgccgact ttggcgcggc agaaatccac gccctgctct ttcagggcga tttccatcgc
    15121 catattggtc atgaccgtgc cgaccacgcc gccgatgttg ataccttctc gggcgcgggc
    15181 tttagcaatg acgtaaatca ggctgtcgcc gtcgtaaacc ttaccgtttt tatcgaccat
    15241 catcaggcgg tctccgtcgc cgtctaaggc gatgccgtag tcggcttcat gctgtaaaac
    15301 ggctgcctgg agtgtcttgg tataagtcgc accgcatttt tcattgatat tgtagccgtt
    15361 gggttcgtcg ccgatgctga cgacttgtgc gcccagttcg tgaaacacct tgggtgcgac
    15421 accgtacccc gcgccgttgg cagtatcgat aaccaacttc aaaccccgaa ggtcggaatg
    15481 gctgggaaag gtagatttgc aaaattcgat atagcggtcg tccgcaccac tgatgcggcg
    15541 tgcgcgaccg aggcgtgcag aaggctgggt tttcatttct tcgtcgattt tggcttcgat
    15601 ttccaactcg atttcatcgc tcagtttcac gccgccctcg gcaaagaatt tgatgccgtt
    15661 gtcggaatag gcgttgtgcg acgcggaaat catcacgccg gcggacaggc gcaacgcgcg
    15721 ggtcagataa gccacgccgg gcgtgggcag cggtccggtc tgtaccacat tcacacccgc
    15781 cgccgtaaaa ccggccacca aagcggcttc cagcatatag ccggaaatgc gcgtgtcttt
    15841 gccgatgagg acggtcggtt tctggtcggt gtcgtgctgc accaaaacct gccccgccgc
    15901 atagccgagt ttcaatacga aatcgggcgt aatcggaaac tgcccgactt cgccgcgcac
    15961 gccgtccgtg ccgaaatatt tttttgccat atgttgctcc gagaatgtga accgttgtcc
    16021 gagattatac agtctgtttg tgccctgctg tctgcaccgt tgatgccgtc tgaagccgcc
    16081 ccgtcctttt cagacggcat gaagtatgcg aaccgctgtt tacagattga tgcccaacgc
    16141 ttcccacacc ttcaacgcat ccgccgtcgc cttcacatca tgcacccgca cgatttgcgc
    16201 gccgcgcgcc acggaagcca gcgccgccgc cacgctgccg tgtacgcgtg ccgccgcgtc
    16261 ggtttcgccg gtcagctcgc ctatcatgct cttgcgcgat acgccgataa gcagcggcaa
    16321 accggtttcc gccatcaatt cgggcaaatg ccgcatcagc gcgatattgt gttgcagggt
    16381 tttgccgaag ccgaaacccg gatcgagcgt gatgcgttgc ggggcgatgc ctgccgcaat
    16441 acattccgcc gcccgcgctt tcaaataccg cgtcacttca ccgacaacat cctgatattt
    16501 cggattaatc tgcatgtttt taggcaaacc ctgcatgtgc atcaggcaaa tgcccgtatc
    16561 cgcctgacgc gccagcaatt cgagcgcgcc ttcgtcggtc aacgccgcca catcgttaat
    16621 aatatcgatg ccgccgagtg ccaacgcttt ttccataacc gccgtgtggc gcgtgtccaa
    16681 actgacggga acaccccacc ccgccacttc cgccaaaaca ggcgcaaccc gcgcccattc
    16741 ttcttcaggc gaaacataat ccgcacccgg ccgcgtcgat tcgccgccga tgtcgagaat
    16801 gtccgcaccc tctttcaaaa gccgttcggc gtgcgccaaa gcggtttggg cgttttgcga
    16861 atacgcgccg ccgtcggaca aagaatcggg cgtgagattc acgatgccca tgattttcgg
    16921 cttgtccaaa ccgatttcaa accgtcctgc ctgccaaccg tgtcgtgcca tctgaactcc
    16981 tcccaaaata aaaaacagat tatatgccgt ctgaaaccgt cttgtgcgct tcagacggca
    17041 ccgctattcg ggcggcagac ggcatgttgt ccgaatatct gctccgcctt tgaatctgcc
    17101 ggtatgcctg ctatccgccc gacttttcaa aacaggttcc gacgattccg cacgcgcctg
    17161 ccgcctttgc caagccgtac aggatttcct gcggcatatc gcggttccat aatcccgtaa
    17221 tattcgcaat cacgggcaga tggctgattt ggcggacttt cacgatggat tcgacatcca
    17281 aacggtaggg atggtctttg gtatggctca acacgcccga ctcgcccaga atcaggtggc
    17341 ggttgccgcg cgagacgaca tattctgcgg cattcaacca atcttcggca gaatggtgct
    17401 tgtctttaca cagcaccaag ggaatgttca ggtttccggc ttcattcaat acggcaagat
    17461 cggacatcag cccgccgccc aaatacagga tgtccgcccc cgcattcaaa gccgcttcga
    17521 catggcggac gttgcggacg cgcaccaata cgggtttccc tgcgccatgc gccgatgcgg
    17581 tctgttccgc caaccgtctg caccgtcccc gcccttcatc cgcacttgaa gtgtcgtata
    17641 agtttgccga agtgaaaaac ggatctagaa acactgcatc cgcattgcgc catactgacg
    17701 gttctgcggc gatacggacg gtttccccgc cgccgaaagc cacgcctttg gcggcaacgc
    17761 ggctgtcttc cgcccgattt tcccgactga cggttttcca tgtatccaaa atgcggacgg
    17821 ctttctcgac ctccggcagc gtctgcacct ccctgacgct caaaacccta tcgtcgccga
    17881 ttgcgccgat gacggtacgc tcgtcgccgt gagaaatgtg ttctcgcaga cctctgctgc
    17941 ggataaaggc gacaacgccg gcaatgtccg cttcggcggc acgcctgctc atgacaataa
    18001 tcatatttcc tcctgacaca agaaacggcc tgcccaaaat aggatttttg caagccgtgt
    18061 tatactgtgg cgtgttttac agattgttcg ggctatggat ttattatcgg ttttccacaa
    18121 ataccgtctg aaatatgcgg tggccgtgct gacgatactg cttttggcgg cagtcgggct
    18181 gcacgcttcc gtatatcgca ccttcacgcc tgaaaacatc cgcagccgcc tccaacaaag
    18241 cattgcacac acacaccgga aaatctcgtt tgatgcggac attcagcgca ggctcctgcc
    18301 ccggccgacc gtcatcctga aaaacctgac cattaccgaa cccggcggcg accagactgc
    18361 cgtttccgtc caagaaacca aaatcggatt gagctggaaa aacctgtggt cggatcagat
    18421 acagattgaa aaatgggtgg tttcgagtgc ggaacttgcc ctgacgcgcg acgggaaagg
    18481 tgtttggaac atccaagacc tgatcgacag ccaaaaacgc caagcctcag tcaaccgcat
    18541 tatcgtcgaa aacagcaccg tccgcctcaa tttcctgcag gaacagctta tcctgaagga
    18601 aatcaacctc aacctgcaat cccccgattc gtcggggcag ccgtttgaaa gttcgggcat
    18661 actggtttgg ggaaagctgt ccgtcccgtg gaaaagcagg gggctgttcc tttcagacgg
    18721 catcggcacg cccgaaatct caccgttcca ttttgaagct tccacttcgc tggacggaca
    18781 cggcatcacc atttccacca ccggcacacc cgccatccgt ttcaacgccg gcggagcgga
    18841 tgccgccggc ctcggcctgc gtgcagacac ttccttccgc aacctccacc tgaccgcgca
    18901 aatccccgcg ctggcactca ggaacaacag cattaaaatt gaaaccgtca acggcgcatt
    18961 taccgccggc ggcgaatatg cccgatggga cggttcgttc aaactcgaca aagccaacct
    19021 gcactccggc atcgccaaca tcggcaacgc cgaaatctcc ggcagcttca aaacaccgcg
    19081 ccaccagacc aacttctccc tcaattcgcc gctcgtatgg acggaaaaca aagggctgga
    19141 cgcgccgcgc ctgtatgtat cgacccttca ggataccgtc aaccgcctgc cgcaaccccg
    19201 tttcatcagc cggctcgacg gttcgctgtc cgtaccgaat ctgcaaaatt ggaatgccga
    19261 attaaacggc acattcgacc gccaaaccgt tgccgcgaaa ttcagataca cacatgaaga
    19321 cgcaccgcat ctggaagccg ccgtcgcact gcaaaaattg aacctgaccc cctatcttga
    19381 cgacgtgcgg caacaaaacg gcaaaatatt tcccgacacc ctcgccaagc tgtccggcga
    19441 catcgaggcg cacctgaaaa tcggaaaagt ccaacttccc ggcctgcaac tggacgatat
    19501 ggaaacctac ctccacgccg acaaaggcca tatcgcgctc agccgtttca agtcagggct
    19561 ttacggcggc cataccgaag gcggcatcag catcgccaac acccgtcccg ccacttaccg
    19621 cctgcaacag aatgcaagca acatccaaat ccaaccgctg ctgcaagacc tgttcggctt
    19681 ccacagcttc agcggcaacg gcgatgcggt catcgacctg accgcgggcg gcgaaacccg
    19741 aaaagagctt atccgctcgc ttcagggcag cctgtcgctg aatatttcca acggcgcgtg
    19801 gcacggtatc gacatggaca atatcctgaa aaacggcatt tcgggcaaaa ctgccgacaa
    19861 tgccgcaccc agcacaccct tccaccgatt cacgctcaac agcgaaattt cagacggcat
    19921 cagccgccac atcgataccg aactcttctc cgacagcctc tatgttacca gcaacggcta
    19981 taccaatctg gatacgcagg aattgtctga agatgtcctt atccgcaacg ccgtccatcc
    20041 gaaaaacaaa ccgattcccc tgaaaatcac cggtacggtg gacaaaccgt ccattaccgt
    20101 cgattacggc aggctgaccg gcggcatcaa ttcgcgcaaa gagaaacaga aaatcctcga
    20161 agacaccctg ctggaacaat ggcagtggct caaacctaaa gaaccgtaaa catcctgcgt
    20221 acaaaaatgc cgtctgaaac acccccgcgc ttcagacggc atgaccgtaa aacctacaac
    20281 cccaattcct cccaaatccc atcaatctta gccgtaaccg cagggtcttt tttgatgacg
    20341 cgtccccatt cgcggtcggt ttcgcccggc catttgttgg tcgcatccaa acccattttg
    20401 ccgccgagtc cgctgacggg gctggcgaag tcgagataat cgatgggcgt gttttctacc
    20461 aaaacagtgt cgcgcacggg gtccatgcgc gtggttaccg cccagatgac ttctttccag
    20521 tcgcgcacgt tcacatcgtc atccaccacg atgatgaatt tggtatacat aaactggcgc
    20581 aggaacgacc agcagcccat catcacgcgc ttggcgtgtc cggcgtactg ttttttcatg
    20641 ctcaccaccg ccatgcggta ggagcagcct tcgggcggca ggtagaaatc ggtgatttcg
    20701 gggaactgct tttgtaaaag cggtacgaac acttcgttca acgccacgcc caaaacagcg
    20761 ggttcatcgg gcggtttgcc cgtgtaggtg gaatggtaaa tcgggttttc gcgcatggtg
    20821 atgcgttcga ccgtgaacac gggaaaatga tcctgctcgt tgtaatagcc cgtgtggtcg
    20881 ccgtatggac cttccaacgc ggtttcgttc ggatgaatca cgccttccaa cacgatttct
    20941 gcgcgtgcag gcacttgcaa atcgttgccg atacatttca ccagctccgt ccgcgaaccg
    21001 cgcagcagtc cggcaaactg gtattcgctc aaggtatcgg gaacaggcgt taccgcgccc
    21061 aaaatggtgg cggggtcgca gccgagcaca acggagacgg gatacggcgt atcaggattg
    21121 agtttgcgga attcctgata atccaacgcg ccgccgcgat gcgacagcca acgcataatc
    21181 agcttgtttt tgccgatgag ttgttggcgg taaatgccga gattttggcg ttttttgtgc
    21241 ggcccgcgcg tgacggtcaa gccccatgtt accagcggcg caacgtcttc cggccagcaa
    21301 tgctgaatcg gaagttgata caaatcaacg tcttcgcctt cccacacgat ttcctgacac
    21361 ggcgcgtttt tcaccacgtt cggcgccatg ctccaaatgt ctttcaacag cggcagtttg
    21421 gaaaacgcgt ctttaatgcc tttgggcggt tcgggttctt tcaaatacgc cagcgtctgc
    21481 ccgatttcgc gcagcttgga cacgctgtcc gcgcccatgc ccatcgccac acgttcgggc
    21541 gtgccgaaca ggtttgccaa cacaggataa ccgtagcgcg taccgtcggg cttaaccggg
    21601 ttttcaaaca gcaacgccgg cccttcggcg cgcagcacgc ggtcggcgat ttcggtcatt
    21661 tccaaatgcg gggaaacggg gtgcgcgatg cgtttgagtt tgccctgctg ctcgagcatg
    21721 gcgatgaagt cgcgcaggtc tttgtatttc atatttatcc tttttgtcct tttatcctga
    21781 acaatccgat tcggataccg ctcctatcct tgcctgcgct tcggcatatt ctatgccgtg
    21841 ataaaagtcg cgtaccagcg gatgttcgct gccttgatgg agttgcaaca aaggacgttg
    21901 accatcgggt tgggtaacga cattgcaatg caaaccgaag gtgtcggttt cataaggggg
    21961 cagccggttg cagatcatgc cgaaataaac ggcgttttcc ggattgtcgt gaaagtggct
    22021 ttgatagtcg ttaaaactct tttcgctgac ggacacccac acgccatatt ccagcgtttc
    22081 ctcatgcccg ataatcggaa tcggcagcac cgcgcggata aagcggtcgg tttggtcggg
    22141 atagcggatg atgcagaaat cagaatcgca ttctgcttga taagcaatgc gttcttcttc
    22201 actgagttga ttatagggat cgggggcggt aaagccgatt gcgggcatat cttcatgttt
    22261 ttcgccgcag gaagtgcaag tgtacataag gtttcagact ttcaaaacga gtttgcggta
    22321 aagccattcg ccggcaaaca gcatccccat caatacatag gcaatcacgc ctgtataaac
    22381 cgcccaccaa tcatatcgcc ccaaccgggc caacaaagcg gcaagcgtcc cgttggcaat
    22441 aaagaatacg caccaaacct gcgttacccg gcgggtatag cgcacggctt tttcaggcag
    22501 gtcgggctgt tgcagccgcg cgagcctttc tatgaccgtc tgtccggcaa acaggctgcc
    22561 gccgaacacc gccaacatca tcagattgac gaggacggga taccaataca tcgaatcatg
    22621 ccgcccgaac accaatactg cggcaaaaaa cagtgtaata aacaaagccg cataacgctg
    22681 ttggggcagt ttggcagtca gggcgcgcag cagccacaac ccgcacatcg ccgccgccag
    22741 ccagacaaac cagcccgcct ccctgccgta atatagtgga ttaacaaaaa ccagtacagc
    22801 gttgcctcgc cttgccgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    22861 cactatacca caaagcggga taggcaatgc ttaatacggt cagaaaaata tgtccgaaaa
    22921 aaccgggttt cattttgaat ccgcacaaat gttttcagac ggcatccgat aaaaacatgc
    22981 cgtctgaaaa ataattagaa atacccgatt agcccgcctg aatcttcaat accgcctgta
    23041 ccacgtcgtt gacggtgcgg acattgcgga aatcttcagc ctgcagcttg cgggctgcca
    23101 cagccattca cgattgcgat atacccggcg gcattcccga ctgccgttca gcagaaagcg
    23161 cacccattcc aaaccgctcc aatatgcctc gggcagcata ccggcttcag acggcatatc
    23221 gtccgaagca gataaagtca ggctgtaacg cgtccctttg gtcagaacca tcgccaaagc
    23281 ataagcaaac ggcgcgcgcg ttgccgatac ggcatatcct tccggcagcg gatcgtccgc
    23341 cgccaaaacc aaaaccgacc cgcatccctc ttccaacagt gatgccgctt ccgccaatgc
    23401 cgtttccaca ccgtcggcac acacggccaa tgccgtctgc tcgctcatat cccgcctcaa
    23461 tatcgaccat taccccgccg ttgcattgtg caccgacaaa ccgaacgaag tcggcgacac
    23521 ggtttgcgat ttcaacagtt ccaaccacaa atcgaaactg cgcgccattt ccccgtcgtg
    23581 cgaggcataa actatagtgg attaaatttt aggggctgta ctagattagc agatatgtta
    23641 ccctcgaaat atgaagataa cacactgcaa atgaaagaaa aaagtacaga aagaactgct
    23701 ccgttttttg t
//
LOCUS       DAWKQJ010000011        23600 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000011 DAWKQJ010000000
VERSION     DAWKQJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.011"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            294..938
                     /gene="rplC"
                     /locus_tag="WJL20_000337"
     CDS             294..938
                     /gene="rplC"
                     /locus_tag="WJL20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ7682535.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            938..1558
                     /gene="rplD"
                     /locus_tag="WJL20_000338"
     CDS             938..1558
                     /gene="rplD"
                     /locus_tag="WJL20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ7682536.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1555..1869
                     /gene="rplW"
                     /locus_tag="WJL20_000339"
     CDS             1555..1869
                     /gene="rplW"
                     /locus_tag="WJL20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ7682537.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1875..2708
                     /gene="rplB"
                     /locus_tag="WJL20_000340"
     CDS             1875..2708
                     /gene="rplB"
                     /locus_tag="WJL20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ7682538.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2715..2993
                     /gene="rpsS"
                     /locus_tag="WJL20_000341"
     CDS             2715..2993
                     /gene="rpsS"
                     /locus_tag="WJL20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ7682539.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3002..3331
                     /gene="rplV"
                     /locus_tag="WJL20_000342"
     CDS             3002..3331
                     /gene="rplV"
                     /locus_tag="WJL20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ7682540.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3341..4033
                     /gene="rpsC"
                     /locus_tag="WJL20_000343"
     CDS             3341..4033
                     /gene="rpsC"
                     /locus_tag="WJL20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ7682541.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4017..4433
                     /gene="rplP"
                     /locus_tag="WJL20_000344"
     CDS             4017..4433
                     /gene="rplP"
                     /locus_tag="WJL20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ7682542.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4433..4624
                     /gene="rpmC"
                     /locus_tag="WJL20_000345"
     CDS             4433..4624
                     /gene="rpmC"
                     /locus_tag="WJL20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ7682543.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4624..4887
                     /gene="rpsQ"
                     /locus_tag="WJL20_000346"
     CDS             4624..4887
                     /gene="rpsQ"
                     /locus_tag="WJL20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ7682544.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5109..5477
                     /gene="rplN"
                     /locus_tag="WJL20_000347"
     CDS             5109..5477
                     /gene="rplN"
                     /locus_tag="WJL20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ7682545.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5489..5812
                     /gene="rplX"
                     /locus_tag="WJL20_000348"
     CDS             5489..5812
                     /gene="rplX"
                     /locus_tag="WJL20_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ7682546.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5822..6361
                     /gene="rplE"
                     /locus_tag="WJL20_000349"
     CDS             5822..6361
                     /gene="rplE"
                     /locus_tag="WJL20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ7682547.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6364..6669
                     /gene="rpsN"
                     /locus_tag="WJL20_000350"
     CDS             6364..6669
                     /gene="rpsN"
                     /locus_tag="WJL20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ7682548.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6685..7077
                     /gene="rpsH"
                     /locus_tag="WJL20_000351"
     CDS             6685..7077
                     /gene="rpsH"
                     /locus_tag="WJL20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ7682549.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7091..7624
                     /gene="rplF"
                     /locus_tag="WJL20_000352"
     CDS             7091..7624
                     /gene="rplF"
                     /locus_tag="WJL20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ7682550.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7638..7991
                     /gene="rplR"
                     /locus_tag="WJL20_000353"
     CDS             7638..7991
                     /gene="rplR"
                     /locus_tag="WJL20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ7682551.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8010..8528
                     /gene="rpsE"
                     /locus_tag="WJL20_000354"
     CDS             8010..8528
                     /gene="rpsE"
                     /locus_tag="WJL20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ7682552.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8521..8706
                     /gene="rpmD"
                     /locus_tag="WJL20_000355"
     CDS             8521..8706
                     /gene="rpmD"
                     /locus_tag="WJL20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ7682553.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8708..9142
                     /gene="rplO"
                     /locus_tag="WJL20_000356"
     CDS             8708..9142
                     /gene="rplO"
                     /locus_tag="WJL20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ7682554.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9154..10464
                     /gene="secY"
                     /locus_tag="WJL20_000357"
     CDS             9154..10464
                     /gene="secY"
                     /locus_tag="WJL20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ7682555.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10469..10687
                     /gene="infA"
                     /locus_tag="WJL20_000358"
     CDS             10469..10687
                     /gene="infA"
                     /locus_tag="WJL20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ7682556.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10708..10821
                     /gene="rpmJ"
                     /locus_tag="WJL20_000359"
     CDS             10708..10821
                     /gene="rpmJ"
                     /locus_tag="WJL20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ7682557.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10887..11249
                     /gene="rpsM"
                     /locus_tag="WJL20_000360"
     CDS             10887..11249
                     /gene="rpsM"
                     /locus_tag="WJL20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ7682558.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11269..11664
                     /gene="rpsK"
                     /locus_tag="WJL20_000361"
     CDS             11269..11664
                     /gene="rpsK"
                     /locus_tag="WJL20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ7682559.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11684..12304
                     /gene="rpsD"
                     /locus_tag="WJL20_000362"
     CDS             11684..12304
                     /gene="rpsD"
                     /locus_tag="WJL20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ7682560.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12330..13316
                     /gene="rpoA"
                     /locus_tag="WJL20_000363"
     CDS             12330..13316
                     /gene="rpoA"
                     /locus_tag="WJL20_000363"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ7682561.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13340..13708
                     /gene="rplQ"
                     /locus_tag="WJL20_000364"
     CDS             13340..13708
                     /gene="rplQ"
                     /locus_tag="WJL20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ7682562.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13855..14568
                     /gene="minC"
                     /locus_tag="WJL20_000365"
     CDS             13855..14568
                     /gene="minC"
                     /locus_tag="WJL20_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ7682563.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14597..15412
                     /gene="minD"
                     /locus_tag="WJL20_000366"
     CDS             14597..15412
                     /gene="minD"
                     /locus_tag="WJL20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ7682564.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15416..15679
                     /gene="minE"
                     /locus_tag="WJL20_000367"
     CDS             15416..15679
                     /gene="minE"
                     /locus_tag="WJL20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ7682565.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15683..16603
                     /gene="oxyR"
                     /locus_tag="WJL20_000368"
     CDS             15683..16603
                     /gene="oxyR"
                     /locus_tag="WJL20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ7682566.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16600..16806)
                     /locus_tag="WJL20_000369"
     CDS             complement(16600..16806)
                     /locus_tag="WJL20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682567.1"
                     /translation="MPYWFLLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKID
                     DDGNILAYRVVGSGKWRFENNACA"
     gene            complement(16933..19770)
                     /locus_tag="WJL20_000370"
     CDS             complement(16933..19770)
                     /locus_tag="WJL20_000370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ7682568.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLANNPAEGVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPGFALPEKYANLDRFVARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGTVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPGMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEVALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESEDAPIAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNTKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="WJL20_000371"
     CDS             complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="WJL20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ7682569.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(20857..22113)
                     /locus_tag="WJL20_000372"
     CDS             complement(20857..22113)
                     /locus_tag="WJL20_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ7682570.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22192..23583)
                     /locus_tag="WJL20_000373"
     CDS             complement(22192..23583)
                     /locus_tag="WJL20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7682571.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
BASE COUNT         6120 a         4985 c         6023 g         6472 t
ORIGIN      
        1 tttgacgaac tgagcatccc cgattacagt catcaaccat attccggttt gtcggagaaa
       61 gatgcatacg ctgtgatgac cggataccga ccctttaaaa gagtctgacc ctatgccgtc
      121 tgaaaattca aaacgcttca gacggcatat tgaagatatt tctgatattt ctgttgatat
      181 ttctttaact tgtcagatat aatgccgagc ttggtacatt tgtgccaagt ttaactttgt
      241 ctgaaagaca ggccaatcgt agcctgtccc tttactttaa aaggaaaata atcatgactt
      301 taggtctggt tggacgcaaa gttggtatga cccgcgtgtt cgacgaacag ggtgtttctg
      361 ttccggtaac cgttttggat atgtctgcca accgcgttac acaagtaaaa tccaaagata
      421 ctgacggcta tactgccgtt caagttacct ttggtcagaa aaaagccaat cgtgtcaaca
      481 aagccgaagc cgggcacttt gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt
      541 ttgctttgac tgaagaaaaa ctggctgaat tgaaagctgg tgacgaaatc accgtttcta
      601 tgtttgaagt cggtcaactg gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg
      661 gcacgattaa acgtcataac ttcggcgccc aacgtacttc ccacggtaac tcccgctctc
      721 accgcgttcc aggttctatc ggtatggcac aagacccggg tcgcgtgttc cccggtaagc
      781 gcatggccgg ccaatacggc aacaccaagg caactgttca aaaattggaa gttgtccgtg
      841 ttgatgcaga acgccaactg ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg
      901 atgttgtagt tcgtcccagc gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc
      961 taaaggacaa gtttcaggca gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa
     1021 tgaagcgttg gttcatcagc tggtaaatgc ctacttggca aacgcccgct ccggtaaccg
     1081 cgctcaaaaa acccgtgccg aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg
     1141 taccggccgt gcccgttccg gtatgacttc ttctccgctg tggcgtaaag gtggtcgcgc
     1201 gttcccgaac aaacccgacg aaaacttcac tcaaaaagta aaccgcaaaa tgtaccgtgc
     1261 cggtatggcg actattctgt cccaattgac tcgtgacgag cgtttgtttg cgattgaggc
     1321 gttgactgcc gaaactccta aaaccaaagt ttttgctgaa caagtgaaaa atctgggtct
     1381 agagcaagtg ttgtttgtaa ccaaacagct cgacgagaat gtttacttgg cttcacgcaa
     1441 cttgccaaac gtgttggttt tggaagctca acaagttgat ccttacagct tgctgcgtta
     1501 caaaaaagta atcatcacta aagatgcagt tgcacaatta gaggagcaat gggtatgaat
     1561 caacaacgtt tgactcaagt gattttggca cctatcgttt ctgaaaaaag caacgtattg
     1621 gctgaaaaac gcaaccaaat gacgtttaaa gttttggcaa atgcaaccaa acctgaaatc
     1681 aaagcggctg ttgagctgct gtttggtgtt caagttgctt ctgtaactac cgttacaact
     1741 aaaggcaaaa ctaagcgttt tggtcgtact ttgggccgcc gcagcgatgt taaaaaagct
     1801 tatgtaagct tggctgccgg tcaagagttg gatttggaag ccgctgctgc agctgcagat
     1861 aaggaataaa caaaatggca atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca
     1921 tggttcgcgt ggtaacagaa ggtttgcaca aaggtgcgcc ttatgcacct ttgctcgaaa
     1981 agaaaaattc tactgccggt cgtaacagta atggtcatat caccacccgt cacaaaggcg
     2041 gcggtcataa acaccattac cgtgttgtag actttaaacg taacaaagac ggcattcctg
     2101 ctaaggtaga gcgtatcgaa tacgatccta accgtactgc cttcatcgca ctgttgtgct
     2161 atgcagacgg cgagcgtcgt tacatcatcg ctcctcgcgg tattcaagtc ggtgccgtat
     2221 tggtttccgg tgctgaagct gccatcaaag taggcaacac cctgccgatc cgcaacattc
     2281 ccgttggtac gactatccac tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac
     2341 gttctgccgg tgcttctgcg gtattgctgg ctaaagaagg cgcgtacgct caagtccgtc
     2401 tgcgctctgg cgaagttcgt aaaatcaacg taaattgccg tgcgaccatc ggtgaagttg
     2461 gtaacgaaga gcaaagcctg aaaaaaatcg gtaaagccgg tgccaaccgt tggcgcggta
     2521 ttcgtccgac cgtacgcggt gttgtcatga atcctgtcga tcacccgcat ggtggtggtg
     2581 aaggccgtac gggcgaggcc cgcgaaccgg tcagcccatg gggtactcct gctaaaggct
     2641 accgcactcg taataacaaa cgcacggata acatgattgt tcgtcgccgt tactcaaata
     2701 aaggttaatt tagtatggct cgttcattga aaaaaggccc atatgtagac ctgcatttgc
     2761 tgaaaaaagt agatgctgct cgcgcaagca acgacaaacg cccgattaaa acctggtctc
     2821 gtcgttctac cattctgcct gattttatcg gtctgaccat tgctgtgcac aacggccgca
     2881 cccatgtgcc tgtgtttatc agcgacaata tggttggtca taaattaggc gaattctcat
     2941 tgacccgtac ctttaaaggc cacttggccg ataaaaaggc taaaaagaaa taaggtgaat
     3001 catgagagta aatgcacaac ataaaaatgc ccgtatctct gctcaaaagg ctcgtttggt
     3061 agctgatttg attcgtggta aagacgttgc ccaagctttg aatattttgg ctttcagtcc
     3121 taaaaaaggt gccgagctga ttaaaaaagt attggagtca gctattgcta atgccgagca
     3181 caataacggt gcggacattg atgaactgaa agtggtaact atctttgttg acaaaggccc
     3241 aagcttgaaa cgttttcaag ctcgcgccaa aggtcgcggt aaccgcatcg aaaaacaaac
     3301 ttgtcatatc aatgtgacag tgggtaacta aggaaaagct atgggacaaa agattaaccc
     3361 tacaggcttt cgcctggcgg taactaaaga ctgggcttca aaatggtttg ctaaaagcac
     3421 cgacttttct actgttttga agcaggatat cgatgttcgc aattatttgc gtcaaaaatt
     3481 ggccaatgct tcggttggtc gagtggttat tgaacgccct gcaaaatctg cacgcattac
     3541 cattcactcc gctcgtccgg gtgtggttat cggtaaaaaa ggtgaggata tcgaggtttt
     3601 gaaacgtgac ttgcaagtct tgatgggtgt acctgttcat gtaaatattg aagagattcg
     3661 ccgtcctgag ttggatgctc aaattattgc tgacggtatt gcccagcagt tggaaaagcg
     3721 cgttcaattc cgtcgtgcta tgaaacgagc aatgcaaaat gcaatgcgtt ctggtgctaa
     3781 aggcattaag attatgactt caggccgtct gaatggtgcg gatattgccc gtagcgaatg
     3841 gtatcgtgaa ggtcgtgtgc cactgcatac tttacgtgca aatgtagatt atgcaaccag
     3901 cgaagcgcac accacatatg gtgtattggg tctgaaagtt tgggtttata cggaaggcaa
     3961 tattaaatct tccaaacctg aacatgagag taaacaaaga aaggcaggta gacgtaatgc
     4021 tgcagccaac tagactgaaa taccgtaagc aacaaaaggg tcgcaatact ggcatcgcta
     4081 ctcgcggtaa taaggtaagt ttcggtgagt tcggcttgaa agccgtaggt cgtggtcgtt
     4141 tgactgcccg tcaaatcgaa gctgctcgtc gtgcaatgac ccgtcatatc aaacgtggtg
     4201 gtcgtatttg gattcgtgta ttccctgata aaccgattac tgaaaagcct attcaagttc
     4261 gtatgggtgg cggtaaaggt aacgtggaat attacattgc cgaaattaaa ccaggtaaag
     4321 tgttgtatga aatggatggc gttccagagg aactggctcg tgaagcattc gagttggctg
     4381 ctgccaaatt gcctattcct acaacctttg tagtaagaca ggtgggtcaa taatgaaagc
     4441 aaatgaattg aaagacaaat ccgttgagca gttgaatgca gatttgttgg acttgttgaa
     4501 agctcagttt ggcttacgta tgcaaaacgc taccggtcaa ttaggcaaac caagtgaatt
     4561 gaaacgtgta cgtcgcgata ttgctcgtat taaaaccgtt ttaactgaaa aaggtgctaa
     4621 gtaatgagcg aaactaaaaa tgttcgtact ttgcaaggca aagtagtaag cgacaaaatg
     4681 gataaaaccg taacagtatt ggttgagcgt aaagtaaaac atccgctgta tggtaagatt
     4741 attcggttat ctactaaaat ccatgcccat gatgaaaata atcaatatgg aattggtgat
     4801 gtggttgtta tatcggaatc ccgtccattg tcaaaaacta aatcttgggt tgtcagtgag
     4861 ctggttgaga aagcacgttc tatttaagaa ttaaagcaac gtgcttggaa tgggaaacga
     4921 agtattgcag caaatttaat ttgcgtgtaa acttcgtttc ctgtctttca gtttcttctg
     4981 gaagtttctt ccctttcggg gtccaagact ggtttacttg aaccgcaagg tttcatttaa
     5041 taagcagcgg ctttgctgta agttatctga aagtggtaaa ttaagttggt taatttaaag
     5101 gtaataacat gattcaaatg cagaccatct tagatgtggc tgataactct ggtgcgcgtc
     5161 gcgtaatgtg tatcaaggta ttgggcggat ctaagcgtcg ctacgcttct gttggcgata
     5221 ttattaaagt ggcagttaaa gatgcggctc cgcgtggccg tgtcaaaaaa ggcgatgtat
     5281 ataatgcggt agttgttcgt actgctaagg gtgtacgtcg tcctgatggt gcgttaatta
     5341 aattcgataa caatgccgcc gtgttactga ataataaact tgaacctttg ggtactcgta
     5401 tctttggtcc ggtaacccgt gaattgcgta ctgagcgatt tatgaaaatc gtttcattgg
     5461 cacctgaagt attataagga atggcacgat gaataaaatc attaaaggcg atagggttgt
     5521 agtaattgct ggtaaggata aaggtaagca gggtcaagta gttcgagtgt tgggtgataa
     5581 agttgttgtt gagggcgtta atgttgtaaa acgccatcaa aaacctaatc caatgcgtgg
     5641 cattgagggc ggtattatta ctaaagaaat gcctttggat atttctaata tcgcaatcct
     5701 gaatccggaa actaataaag cggaccgtgt tggtattaag ctgattgaaa atgaaggcaa
     5761 agttaaacgc gttcgtttct tcaaatcaaa tggctctatc attggggcat aaggagataa
     5821 catggctcgg ttgagagagt tttataaaga gacagttgtt cctgaattgg ttaaacaatt
     5881 tggttacaaa tcagtaatgg aagtcccgcg tattgaaaaa atcaccttga atatgggtgt
     5941 gggtgaggct gttgctgaca aaaaagttat ggaacatgct gtttccgatt tagagaaaat
     6001 tgccggtcaa aaaccggttg ttactgttgc ccgtaaatct atcgcaggtt ttaaaatccg
     6061 tgataactat ccggttggtt gcaaagtaac attgcgtcgt gatcaaatgt ttgaattctt
     6121 ggatcgtttg attactattg cattacctcg cgtacgtgac ttccgtggtg tgagcggtaa
     6181 atcatttgat ggccgtggca attacaatat gggtgttcgt gagcaaatta tttttccgga
     6241 aattgaatac gataaaattg atgctttgcg tggtttgaat attactatta ctactacagc
     6301 aaaaaccgat gaggaagcga aagctttatt gtcattgttt aaatttccgt tcaaaggata
     6361 atcatggcta agaaagcact tattaatcgt aatctgaaac gtcaagcttt ggctaaaaaa
     6421 tatgcggcta aacgcgcggc aattaaagcg gtaatcaatg attcgaatgc aactgaggaa
     6481 gagcgttttg aggctcgttt gaggtttcaa tccattcctc gtaatgcggc acctgtgcgt
     6541 caacgtcgtc gttgtgcttt gacaggtcgc cctcgtggta ctttccgtaa atttggtttg
     6601 ggtcgtatta aaatccgtga aatcgccatg cgtggcgaaa ttccgggtgt tgttaaagcc
     6661 agctggtaat aggagtaatt aagaatgagt atgcatgatc ctatttccga tatgttgact
     6721 cgtatccgca atgcgcaacg tgctaataaa gcagcggttg caatgccttc ttcaaaatta
     6781 aagtgtgcta ttgcaaaggt attgaaagaa gaaggatata ttgaggactt cgcagtttca
     6841 tctgacgtaa agtctatatt ggaaattcaa ttaaaatact atgcaggtcg tcctgtaatt
     6901 gaacaaatca agcgtgtatc tcgccccggt ttgcgtattt ataaagcgtc tagtgagatt
     6961 ccaagtgtta tgaatggctt gggtattgct attgttagta cttctaaagg tgtaatgact
     7021 gatcgtaaag cacgttctca aggtgttggt ggtgagttgt tatgcattgt agcctagtgg
     7081 aggaaaagaa atgtcacgtg tcgcaaaaaa cccagtgact gttcccgctg gtgtagaagt
     7141 aaaatttgga acagaggcat tagttattaa gggtaagaac ggtgaattgt cttttccttt
     7201 gcattctgat gtagccattg aatttaatga tggcaaattg gcttttgttg cgaataacag
     7261 cagtaaacaa gcaaatgcaa tgtctggtac tgctcgcgca ttagtcagca atatggttaa
     7321 aggtgtttca gaaggttttg agaaaagatt gcaattgata ggtgtgggtt atcgtgctca
     7381 agcacaaggt aaaatcttga atctgtcttt gggtttttct catccgatcg tatatgaaat
     7441 gcctgaaggt gtctccgttc aaactcctag ccaaacagag attgttttaa ccggctcgga
     7501 taaacaagtt gttggtcaag ttgctgctga gattcgtgcg ttccgtgctc ctgagcctta
     7561 taaaggtaaa ggtgttcgct atgtaggaga agtagtggta atgaaagaag ccaagaaaaa
     7621 ataattgagg ttcactaatg gataaacata caacccgact ccgtcgtgca cgcaaaaccc
     7681 gtgctcgtat tgcggacttg aaaatggtaa gattatgtgt gttccgaagc aataatcata
     7741 tttatgctca agtaattagt gctgaaggtg ataaagtatt ggctcaagcc tctacattgg
     7801 aagctgaggt gcgcggtagt ctgaaatctg gaagcaatgt tgaagcagct gcaatagttg
     7861 gtaaacgtat cgctgagaag gctaaagcag caggtgtaga aaaggttgct tttgatcgtt
     7921 caggtttcca atatcacggt cgtgtgaagg ctttggctga agctgctcgt gaaaatggtt
     7981 taagcttcta aatatttgga gactttcaga tggcaaaaca tgaaattgaa gaacgcggtg
     8041 acggtctgat tgaaaagatg gtcgcagtta accgtgtaac caaagtagtt aaaggtggtc
     8101 gcattatggc tttctctgcg ctaactgttg ttggtgatgg agatggtcgt attggtatgg
     8161 gtaaaggtaa gtcaaaagaa gtgcctgttg cagtccaaaa ggcgatggat caagcacgac
     8221 gctctatgat taaggtgcca ttaaaaaatg gtacgattca tcatgaggtt attggtcgac
     8281 atggtgctac taaagtattt atgcagcctg ctaaagaggg tagtggcgta aaagccggtg
     8341 gacctatgcg tttggttttt gatgctatgg gcattcataa tatctccgcc aaagtgcacg
     8401 gatctactaa cccatataat atcgtacgtg caacattaga tggtttgtct aagttgcata
     8461 ctcctgctga tatcgcagcc aaacgtggct tgacagtgga agacattttg ggagttaacc
     8521 atggctgaac aaaaaaagat tagggttaca ttggttaaaa gcctgattgg tacaattgaa
     8581 tctcatcgtg catgtgcacg cggtttaggt ttgcgtcgtc gcgagcatac ggtagaggtt
     8641 ttagataccc ctgaaaaccg tggtatgatt aataaaatca gctacttgtt gaaagtggag
     8701 tcttgatatg tttttgaata caattcaacc tgctgttggt gctacgcatg ctggtcgtcg
     8761 tgttggacgc ggtattggta gtggtcttgg caaaacgggt ggtcgtggtc ataaaggtca
     8821 aaagagccgg tctggtgggt ttcataaggt gggtttcgag ggtggtcaaa tgcccttgca
     8881 acgacgcctc cctaaaagag gttttaaatc tttaacagca tcagctaatg cacagcttcg
     8941 tttaagtgaa ctggaatcaa ttgctgttaa tgagattgat attttggtct taaagcaagc
     9001 gggtctgatt gcatctacag tctctaatgt taaagttatt gcttctggtg aaatttctaa
     9061 ggcagttgct ttgaagggta ttaaagttac caaaggtgcg agagctgcta tcgaggctgt
     9121 tggtggtaag attgaaatgt aaggtttaat attgtggcta atcaacaaac gtcatcaggt
     9181 tcatccaaat ttggagatct taagaaacgt cttttgtttc tatttggagc attgattgtt
     9241 tttcgaattg gtgcccatat acccgtacct ggagttgatg ctgttgcttt agctaaatta
     9301 tacgaaagcg ctggaaacgg catcctggga atattgaata tgttttccgg tgggtcgtta
     9361 gagcgcttta gtatatttgc aataggaatt atgccatata tttcagcttc tattattgta
     9421 cagctcgctt ctgaaatttt gccatcattg aaggctttaa aaaaagaagg ggaggctggt
     9481 agaaaggtaa ttacgaaata tactaggtat ggtactgttt tgttagcaat tcttcaaagt
     9541 ctaggtgttg catctttcgt atttcagcaa ggaattgttg taacaagttc atttgagttt
     9601 catgtttcca cggtagtttc tttggtaacg ggaaccatgt ttcttatgtg gcttggggag
     9661 caaattactg aaaggggtat cgggaacggt atttctttaa tcattacggc aggtattgct
     9721 tcaggtattc cttcgggtat tgcaaagctg gttacactga cgaaccaagg ttctatgagc
     9781 atgcttacgg cgttgtttat tgtatttggt gccttattat taatttattt ggttgtatac
     9841 tttgaaagtg cacagcggaa gattcctatt cattatgcaa aacgccagtt taatggtagg
     9901 gcgggtagtc aaaatacgca tatgcctttc aagttgaata tggctggtgt tattccccca
     9961 atttttgctt ccagtattat tctatttcca tctactcttt taggttggtt tggttcggct
    10021 gatacaaata gtgttttgca caaaatagct ggattgttac aacacggtca attgctgtat
    10081 atggctttat ttgcagcgac agttattttc ttttgttatt tttatacggc tttggttttt
    10141 agccctaaag aaatggcaga gaatttaaaa aagagtggtg cttttgttcc tgggattaga
    10201 cctggtgagc agacctctag gtatttagaa aaagttgtat tacgtttgac attgtttgga
    10261 gctctttata ttacaactat ttgtttaatt ccagagttct taactacggt tttaaatgta
    10321 cctttttatt tgggtggcac gtctttgttg attctagttg ttgtaacgat ggattttagt
    10381 acacaaataa attcgtatag gcttactcaa cagtatgata agttaatgac tcgttcagaa
    10441 atgaaatcat tttctcggaa atagaattat ggcgaaagaa gatactatcc aaatgcaagg
    10501 tgaaattctt gaaactttac ctaatgcaac atttaaagta aaacttgaga atgaccatat
    10561 tgtattgggt catatttctg ggaagatgcg gatgcattac attcgtattt ctccgggaga
    10621 taaggtcaca gtagagctga caccttatga tctaactagg gctcgaatcg ttttcagagc
    10681 aagataaacc aataaaagga aaataaaatg cgtgtacaac catctgttaa gaaaatttgc
    10741 cgaaattgca agattattcg tcgaaatcgt gtagttcgtg taatttgtac tgatctccgt
    10801 cacaaacagc gtcaaggtta atggaatatt tcttttaatg tgattctgtg atatagtgac
    10861 acactttgcc ctaaaaagga aaaaatatgg ctcgtattgc aggggtaaat atccctaata
    10921 acgcacacat cgtaattggt cttcaggcta tttacggtat tggtgctact cgtgctaaat
    10981 tgatttgtga ggctgcaaat attgcgcctg atactaaagc aaaagatttg gacgagactc
    11041 aattagatgc tttgcgtgac caagttgcca agtatgaagt agaaggtgat ttgcgtcgtg
    11101 aggtaactat gagtatcaag cgattgatgg acatgggctg ctatcgtggc ttccgtcatc
    11161 gtcgcggctt accatgccgc ggtcaacgca ctcgtacaaa tgcgcgtacc cgcaaaggtc
    11221 cgcgtaaagc gattgctggt aagaaataaa ttttaaggaa ttttattaat ggctaaagca
    11281 aacacagctt cacgtgtacg taaaaaagta cgtaaaaccg tgagtgaggg tattgtgcac
    11341 gttcatgcat ctttcaacaa taccatcatt acaatcactg accgtcaagg caatgcgttg
    11401 tcttgggcta cctctggcgg cgctggtttt aaaggttctc gtaaaagtac accatttgca
    11461 gcacaagttg cagcagaagc agctggtaaa gttgcccaag agtatggcgt taaaaattta
    11521 gaggttcgta ttaaaggtcc aggtccaggt cgtgaatcct ctgtacgtgc tttgaatgct
    11581 cttggtttca agattaccag cattactgac gttaccccgt tgcctcataa cggttgccgt
    11641 ccgcctaaaa aacgtcgtat ttaatattgg agtgatttga aacatggcac gttatattgg
    11701 ccctaaatgt aagttggcac gtcgcgaagg tacggatttg tttttgaaga gtgcgcgccg
    11761 ctctttggat tctaaatgta aaattgattc cgctcccggt cagcatggtg caaaaaaacc
    11821 gcgtttgtca gactatggtt tgcagttgcg tgaaaaacaa aaaatccgcc gtatttatgg
    11881 cgtattagaa cgtcagttcc gtcgttattt cgcagaagct gatcgtcgta aaggttctac
    11941 cggcgagttg ctgttgcagt tgctggaatc tcgtttggat aatgtcgttt atcgtatggg
    12001 tttcggttct acccgagctg aagcaagaca gcttgtttct cataaggcga tagttgtgaa
    12061 tggacaagtt gtcaatattc cttctttcca agtgaaagct ggtgatgttg tctcggttcg
    12121 tgaaaaagcc aaaaaacagg tacgtattca agaagcattg ggtttggcaa ctcaaatcgg
    12181 cttgccgggt tgggtttctg tagatgcgga taaacttgag ggtgtgttca aaaacatgcc
    12241 ggatcgctcg gaattgaccg gtgatattaa tgaacagctg gtggtagagt tctactctaa
    12301 ataatgctag ctcagtgagg gacagttaaa tgcagaatag cacaaccgaa tttttgaaac
    12361 ctcgtcaaat tgatgtaaat actttttctg caactcgtgc aaaagtatct atgcagccat
    12421 ttgaacgtgg tttcggtcat accttaggta atgctttgcg ccgtatctta ctgtcatcca
    12481 tgaatggttt tgctcctact gaagtagcta ttgccggtgt attacacgaa tattctactg
    12541 ttgatggtat tcaggaagat gttgttgaca ttttgctgaa tattaaaggt attgtgttta
    12601 aactccatgg tcgtagccaa gttcaacttg tgttgaagaa atcaggttca ggtgtcgtat
    12661 ctgccggtga tattgagttg ccgcatgatg tagaaattct gaatcctggt catgtcattt
    12721 gtcatttggc tgataacggt caaattgaga tggaaattaa agtagagcaa ggtcgtggtt
    12781 atcaatctgt ttcaggtcgt caggtagttc gtgatgagaa ccgtcagatt ggtgcaatcc
    12841 agttggatgc gagcttttcg cccatcagcc gtgttagctt tgaggttgaa cctgcacgtg
    12901 tagagcagcg gacggatctt gataagttgg ttttggatat cgaaaccgac ggttctattg
    12961 atcctgagga agctgtacgc agtgcggcac gtattttgat tgatcagatg tctatttttg
    13021 ctgatttgca gggtacgcct gtggaggagg ttgaagaaaa agcacctcct atcgaccctg
    13081 ttcttttgcg tccggtggat gatctggaat tgacagtacg ttcagctaat tgtttgaaag
    13141 ctgaggatat ttattatatt ggcgatttga ttcaacgcac tgaaaccgag cttcttaaaa
    13201 cgccgaattt gggacgtaaa tctttgaatg agattaagga agtattggca tctaaaggtt
    13261 tgacactggg ttctaagttg gaagcatggc cacctgtagg cttggaaaag ccttaatgaa
    13321 gaattaaagg ataattgata tgcgtcatcg taatggcaat cgcaaattaa accgtaccag
    13381 cagtcatcgt gctgcaatgc tgcgtaatat ggcgaattca ttattgactc acgaaactat
    13441 tgtaacaact ctgcctaagg ccaaggaatt gcgccgtgta gtagagccgt tgattacatt
    13501 gggtaaaaag ccgtcattgg caaaccgccg tttggcattt gaccgtactc gcgaccgtga
    13561 tgttgtagta aaactgtttg gcgatttggg tcctcgtttt actgctcgta acggtggtta
    13621 tgttcgggtg ttgaaatacg gattccgtaa aggtgataat gcacctctgg cactggttga
    13681 attggttgac aaaccggctg ctgagtaatt ttagtcatat aacgccatct gccgaaaagc
    13741 aggtggcgtt atttttgcaa tatctgatag gtaatagggt attggctatc atgtttaaaa
    13801 tattaattga atagctaagg tttgcgcggt aaacttacat cattaaaaaa ttctatgatg
    13861 gtttatataa tgaatgcttt cgatataaag tcgacaaaga tggacgtatt gtctatatct
    13921 ttgcatacgt cagacttgtt tgatttggaa gatgtgctgg tcaaattggg caagaagttt
    13981 caagagtctg gtgttgttcc atttgtgctg gatgttcaag agtttgatta tcccgagtct
    14041 ttggatcttg ctgcattggt ttcgttgttt tcaaggcatg gtatgcaaat tttgggtctg
    14101 aagcattcta atgaacgttg ggctgctgcg gctatgaagt atcatttgct gttttgtctg
    14161 tctcattcgg aaaatgttaa agaactgggt caggttgagg tgcagaacac ggaggatggt
    14221 cagaaagcaa ggaaaacagt attgattaca tcccctgtcc gtaccggtca gcaggtttat
    14281 gccgaagatg gcgatttgat tgttacgggg gcggtcagtc agggggcgga attgattgcg
    14341 gatggcaata tacatattta tgcgccgatg agggggcgtg ctttggccgg cgccaagggt
    14401 gatacttctg cccgcatatt tatccactcc atgcaggcag aactggtttc tgtggcgggt
    14461 atttaccgta attttgaaca ggatttgccg gatcatctgc acaagcagcc ggtacagata
    14521 ttgttgcagg ataatcggtt ggttatcagt gcaattggct cagagtaatt gtttgatatt
    14581 ttaaaaagga aatattgtgg caaaaattat tgtagtaact tcaggtaagg gcggtgtcgg
    14641 taaaacgact accagtgcca gtattgcgac aggtttggca ttacgcggat ataaaactgc
    14701 ggtaattgat tttgatgtgg gtttgcgtaa cctcgacctc attatgggtt gcgagcgtcg
    14761 tgtcgtttat gacctgatca atgtcattca gggggaggcg acgctcaacc aggctttgat
    14821 taaagataaa aattgtgaaa acctgtttat tttgccggct tcccagactc gggataaaga
    14881 cgctttgaca cgcgagggcg tagaaaaagt gatgcaggag ctgtccggca agaaaatggg
    14941 ctttgagtat attatttgcg actctcctgc cggtattgag cagggtgcat tgatggcgtt
    15001 gtattttgct gatgaagcca ttgtaacgac caatcctgag gtttccagtg tgcgtgactc
    15061 cgacaggatt ttgggaattt tgcaaagcaa atcccataag gcagagcaag gcggttcggt
    15121 taaagaacat ctgttgatta cgcgttattc tcccgaacgt gtggcaaaag gcgaaatgct
    15181 gtctgtacag gatatttgcg atattctgca tattcctttg ctgggtgtga ttcctgaatc
    15241 ccaaaacgtc ttgcaggcat ccaattccgg agaaccggtc atccatcagg acagcgtggc
    15301 ggcttccgag gcatataagg acgttattgc ccgtcttttg ggcgagaacc gtgaaatgcg
    15361 tttcttggaa gctgagaaaa aaagcttctt caaacgtctg tttggaggat aaggtatgtc
    15421 attaatcgaa tttttattcg gcagaaagca gaaaacggca accgttgccc gcgaccgcct
    15481 tcaaatcatc attgcccaag agcgcgccca agaaggtcag gctccggatt acctgccgac
    15541 tttacgtaaa gagttgatgg aagtcctgtc caaatatgtg aatgtttcat tagacaatat
    15601 ccgtatttcc caagaaaagc aggatggtat ggatgtgctt gagttgaaca ttactttgcc
    15661 ggaacagaaa aaggtatagg acatgacctt aaccgaattg cggtacatcg tcgcagtcgc
    15721 ccaagaacgt catttcggca gggcggcgcg gcgttgtttt gtcagccagc ccactttgtc
    15781 tattgccatt aagaaattgg aagaagagct tgccgtctct ttgtttgacc ggagcagcaa
    15841 cgatattatt acgaccgagg cgggggaacg tatcgttgca caggcgcgta aggtgttgga
    15901 agaggcagag cttatcaggc atttggcaaa tgaagaacaa aacgagctgg agggtgcgtt
    15961 caaactcggg ctgattttta cggttgcgcc gtacctgctg ccgaaactga ttgtctcgtt
    16021 gcgccgtact gcaccgaaaa tgcctttgat gttggaagag aattacacgc atactttgac
    16081 cgagtcgctc aaacgcgggg acgttgacgc gattatcgtt gccgaaccgt ttcaagagcc
    16141 gggcattgtt accgaaccct tgtatgacga accgtttttc gtgattgtcc cgaaagggca
    16201 ttcatttgag gaactggatg ccgtttcgcc acggatgctg ggtgaggagc aggttttgct
    16261 gctgacggaa ggcaactgta tgcgggatca ggtactctca agctgttccg aattggcggc
    16321 gaaacaacgt atacaggggt tgaccaatac attgcagggc agctcgatca atacaatccg
    16381 ccatatggtc gccagcggtt tggcaatcag cgtgttaccg gcaaccgcgc tgaccgaaaa
    16441 cgatcatatg ctgttcagca ttattccgtt tgaaggtacg ccgccaagcc ggcgggtcgt
    16501 attggcgtac cgccgcaatt ttgtccgtcc gaaggcgttg tcggcgatga aggcggcgat
    16561 tatgcagtcg cagcttcacg gggtaagttt tatccgcgac taggcgcagg cattgttttc
    16621 aaaacgccat ttccctgagc cgacaacacg gtatgccaag atattgccgt catcatcgat
    16681 tttgagtata gcatcgccac ggaaactgcc gtcctgaaga tattcgactt ttgcatcact
    16741 gtgaatgttt tcatcagtgc cgatgcaatg ccatgtatag tggattaaca aaaaccagta
    16801 cggcattacc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    16861 atttttgtta atccactata aaagaggccg tctgaaaaac atttttcaga cggccttgtt
    16921 tattcaatca aatcagtctt tcaacttcgc caactgattt tgaacttttg ccattttgtc
    16981 ttccaactcc gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt
    17041 gtagccgggt ttggagagtt tggtgttgag tttgtccaag gctttttgca gcttctcggc
    17101 ttctttgctc aagcgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg
    17161 cgcaccgttg cagacggcga tgggcgcgtc ttcgctttcg ggcagggcgg cgacttgccg
    17221 tgcttcggtc aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtctgc
    17281 gctttcgaca aacagcgggg ctttcacgtt gggctggatg cccatttcgc cgcgcaggtt
    17341 gcggactgcg ccaatcaaat cctgcaacac agtcatttgc tcgaatgccg tctgaacaat
    17401 atcgccgccg tcggcttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttcgcgtc
    17461 gcacataggg gcgacggttt gccacagttc ttcggtaatg aacgggataa tcgggtgcag
    17521 caggcgcagg gcgacttcga gtacgcgcaa taaggtatgg cgtgtggcgc gttggcggct
    17581 ggcgcagccg gtttgaagct gcactttggc aagttccaaa taccagtcgc aatagtcgtt
    17641 ccatacgaag ctgtacaggg tttctgccgc caaatcaaag cggtaggttt cgtaggcttg
    17701 cgtaacctgc tcgatggtct gattcagacg gcctacaatc cacatatcgg ggaaggaata
    17761 gccgcgcggt tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa
    17821 gacgaagttg gtggcgttcc agattttgtt acagaagttg cggtagcctt cggcgcgttt
    17881 gaagtcgaag ttgacgctgc ggccgaggct ggcgtagctc gccatagtga agcgcaatgc
    17941 gtccgcgccc ataccgggga tgccttcggg aaagagtttt ttggtggctt cttccacttt
    18001 cggcgcggtt tcgggtttgc gcaggccggt ggtacgtttt accagcagct tgtccaagtc
    18061 gatgccgtca atcaaatcga cagggtcgat gacgttgcct tctgatttgg acattttttt
    18121 gccttcgtgg tcgcgcacga tgccgtggat gtacacgtct ttaaacggta ctttgccggt
    18181 gaagtgggtg gtcatcataa tcatgcgcgc cacccagaag aagatgattt cgtagccggt
    18241 aaccaagacg ttggacggca ggaaggcttt gagttcgtcg gtttcagacg gccagccgag
    18301 cgtggagaac ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt
    18361 caaaccggtt ttgccggctt gtttttcggc ttcttcctga ttgcgggcaa cgtacacatt
    18421 gccttcgttg tcgtaccatg cagggatttg atgtccccac cacagttggc gcgagataca
    18481 ccagtcttgg atgttgttca tccattggtt gtaagtgttg acccagtttt cggggataaa
    18541 gcgtaccgtg ccgctgtcga cggctttttt ggctttgtcg gcgaggctca agcctttgaa
    18601 ttcgctgtca ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt
    18661 cagcatcggc tcaatgaccg aacctgtgcg gtcgcctttc ggcgtcatca gcgtatgcgc
    18721 tttgatttcg accaagaagc cttgttcctg caaatcggca accatttgtt tgcgcgcgac
    18781 aaagcggtct aagtttgcgt atttttcagg cagggcaaag cctggttgcg cttcgccttt
    18841 gaagttgaac acttcggcgt ttgccagcac tttggcttcc aagtcgaaca cattaaccaa
    18901 gcgcgtgtcg tggcgtttgc cgacttcgta gtcgttgaag tcgtgcgcag gcgtgatttt
    18961 cacgcagccg gtaccgaagt ctttttcgac gtattcatcg gcaatcacgg ggatggtgcg
    19021 gccggtcagc ggcaggatta attccttgcc aatcaaatgg gtataacgtt cgtcttcagg
    19081 attgacggca acggccacgt cgcccagcag cgtttcaggg cgggtggtcg ccacgataac
    19141 gccttcggca ggattgttgg ccagcggata gcggatgtgc cacatagagc cttgttcttc
    19201 cacgctttcc acttccaaat ccgataccgc cgtgccaagc acgggatccc agtttaccaa
    19261 gcgtttgccg cgataaatca agccctgttc atacaggcgt acgaacactt ctgtcacggt
    19321 ttcggcgcgc acgtcgtcca tcgtgaaata ctcgcgcgtc cagtcggcag agcagcccac
    19381 gcggcgcatt tgttgggtaa tcgtgccgcc ggacacttct ttccattccc acactttttc
    19441 caagaatttt tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc
    19501 gaccacgatt tgcgtggcga tgcccgcgtg gtcggtgccg ggaatccagg cggtgttgca
    19561 gcctttcatg cggtagtagc gggtcaggcc gtccataatg gtttggttga aggcatggcc
    19621 catgtgcagc gtgccggtta cgttgggcgg cggcagttgg atggagaaag acggtttggt
    19681 caaatccata tcaggttgga aatagccctg ctcttcccag ttttgataat gtttggattc
    19741 gatttcggct ggattgtatt tgtctaacat gatggaactt tgtgaaatta aggttatttt
    19801 tgatgtgcgg attataacgc aaaaaggccg tctgaatcat ttcagacggc ctttggcata
    19861 caggttttaa aaatggaaca ataccaggct gacggcaatc accgccatgc ccattgtcag
    19921 gccgtaaacg gtttcatggc cgtctgaata gcgtttggca gccggcagca gctcgtccaa
    19981 cgccaaaaac accatcacac cggctatcac gccgaatacc gaaccaaaca cggcaggcga
    20041 caaaaacggc tgcaaaacca aatagcccaa agccgccccc aacggctcgg ccaagccgga
    20101 tagcagacac gcccacaccg ttttcttacg gctgcgggtg gcaaaataaa ccggcgcggc
    20161 gatggaaatg ccctccggaa tattatggat ggcaatcgcc aaggccaaag gcatcccgac
    20221 tgctggattt tccaatgtgg caaaaaacgt cgccaagcct tcggggaaat tgtgcgcagt
    20281 aatcgcaaac gccgccatca tgccgactcg cgcgatatgg cggcgtttgc tttcttgaaa
    20341 cgacgggtct tgcgcgtcta aagtttcatg cgggttcggc accagacggt caatcagcgc
    20401 aatgccgccc atcccggcca aaaatgccat ggtcgccgcc gcaaacgcgt ggtctttatc
    20461 ataaatttca gcgaacgcct cgctggactt actgaaaatc tccgtcaggg aaacatacac
    20521 catcgcaccg ccggcaaatg ccaaaccaaa cgacaacacg cgcggattgg gcgttttgga
    20581 aaacatcacc aagccgctgc ctaatacggt aaacaaaccg gcagccaacg taatggaaaa
    20641 ggcaacggcc aaattggaca tcgaaaaatc gggcatggga aaacctgcgc taaaagctgg
    20701 gacaggttca gactaacact ttttaatgcg tatgataata gttattattt attttattga
    20761 ttggataccc gaattttgaa acaaaaggcc gtctgaaaaa tgattttcag acggccttta
    20821 aatttgaaat gtcgctaaac cttatcgctt tccagcttaa gcctgatagc gcgacaggct
    20881 caaatcgtcg cttcggattt cggtgtcttt gccgctcacg atatcggcgg ttaattttgc
    20941 cgaacccagc gacatggtcc agcccaaagt gccgtggcct gtattcaaga ataagttttc
    21001 aaagcgggtg cggccgatta acggcgtgct gtcgggcgtc atcggtctga ggccgctcca
    21061 gaacaatgct tggctcaaat cgccgccttc cgggaacaaa tcgttgacga ccaaagccaa
    21121 ggtttcgcgg cgtttttcgg gcagtttgat ttcgtagccc gacaattccg ccataccgcc
    21181 gacgcggatt ctgttgtcaa agcgcgtgat ggcgactttg tagctttcat ctaaaacggt
    21241 ggacaccggt gcgccgtcgg aatttgtgac cggcagggtc aaggaatagc ctttgacggg
    21301 ataaatgggc agattgagat ccaactgcgc caaaaccgtc ctgctgaaac aaccgagcgc
    21361 gcagacaacg gcatctgctt caaaccgccc tgtttcggtt tcaacggttt tgatgcgcag
    21421 cccgttgtgg tcgatgcggc tgatggtttg gttgaaatga aaccgtacgc ccttttcctg
    21481 acacaatttg tataggtttt cagtgaagag gcggcagtcg ccggttgcat ccgcaggcag
    21541 gtgcaggccg ccggcaattt tggcggtaac gcgtgccagc gcaggctcga attctgcgca
    21601 ttcttcgggc ttcagacggc ggtacggcac gccgtagcgt tccaaaacgg caatgtcttg
    21661 ttctgccgct tcaacttctt tagcctgacg gaaaatctgc aacgtccctt ttttgcgtcc
    21721 ctcaaaattc atgccggttt gcgcttcaaa acggcggaac atttcacggc tgtattcgga
    21781 catcctgacc atgcgctctt tattgatttg atagcgggat gccgtgcagt tttgcagcat
    21841 ccgccacagc cattcgattt gatacaggct gccgtcgggg cgaaacagca aaggcggatg
    21901 gcttttaaac agccatttca gcgctttggt cggaataccg ggtgcagccc aaggcgtggt
    21961 atagccgtaa gaaagctggc ctgcgttggc aaaactggtt tccatcgcca caccctcggc
    22021 gcggtcgatg accgttactt catgtccggc ctctgccaga taccacgcgg aagacacgcc
    22081 ggcaacaccc gcacctaaaa caagcacttt catgtttctc cctccggctt tttcaaaaca
    22141 gacttaatat gccgtgccgt ctgaagattc ggattcagac ggcctcggat attaatgcgg
    22201 caattcgcca tttgtgattt tttgtttgaa gtcgcgcgtt tcattgacga tgactttcgc
    22261 catcaataaa agtgcaatca ggttgggcaa tgccatcaag ccgttgaatg tgtccgaagc
    22321 cagccacacc aaatcaaggc tcaacacggt acccagcata acggaagaaa cataaccgac
    22381 gcggtacaaa ccggcaaatt tctcgccgaa aacatacacc gcgcattttt cgccgtaata
    22441 gcaccagccg agaatggttg agtaggcaaa gaaaatcagg ccgatggtaa caatccagcc
    22501 gccgatgccg ggcagcattt tttggaatgt gacggttgtc agtgccgcgc cgctcacttc
    22561 aggtttgaca aactcgccgc ccgcgccgag cagtcccatt accaacacga tgccggtaat
    22621 cgagcaaacg acgatggtat ccaaaaacgt accggtcata gaaaccaagg cctgacggac
    22681 gggatggtcg gttttcgcgg ctgcggcggc aataggcgca gaacccatac ccgcctcatt
    22741 ggagaacacg ccgcgcgcca cgccgtagcg gatgaccgta ccgatagcgc cgcccgccac
    22801 tgcctgcgcg ctgaacgcat cggagaaaat cagcttgacg gcaggcatca gtgcatcgga
    22861 attaatcgcg ataatggaaa gaccgcccaa cacataaaac accgccatag caggcacgat
    22921 gaaagaagcg gctttggcga tgcctttaat accacctaaa acgacaacgg cagtcagaac
    22981 ggtcaacgta atgccggtat aggcaggttc gataccgaag ctggtttgca ccgcctgtgc
    23041 aaccgagttg gactgcaccg agctgccgat accgaaggaa gcgaatgtgc cgaacagcgc
    23101 aaacgcgacg gccatccatt tccagttttt gcccaagcct ttttcgatgt aatacatcgg
    23161 gccgccggac atttcgcctt tggaattgtt gacgcggtat ttcaccgcca acacgccttc
    23221 gccgtatttg gtggccatgc cgaaaatggc ggtcatccac atccaaaata ccgcgcccgg
    23281 gccgccggtt accaccgcag tcgccacgcc ggcgatgtta cccgtaccga tggtggcgga
    23341 cagcgcggtc atcaacgccg cgaaatggga aatatcgcct tcgtggcctt cgccgccttc
    23401 atacttcttt gacggcacaa atgcctgttt cagcgcatag cccaacatag tgaactgcaa
    23461 gcctttcagc ataacggtca gcacaatgcc ggtgccgacc agcagcatca acataacaga
    23521 tccccagacc cagccgctga cggtttcgaa aaaggctttg agattgtcta aaaacaattg
    23581 catggctttc tcctttgtct
//
LOCUS       DAWKQJ010000012        22473 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000012 DAWKQJ010000000
VERSION     DAWKQJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.012"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..637
                     /locus_tag="WJL20_000374"
                     /pseudo
     CDS             15..637
                     /locus_tag="WJL20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            673..1434
                     /locus_tag="WJL20_000375"
     CDS             673..1434
                     /locus_tag="WJL20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ7682572.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGNPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDYLREQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1489..1830
                     /gene="fdx"
                     /locus_tag="WJL20_000376"
     CDS             1489..1830
                     /gene="fdx"
                     /locus_tag="WJL20_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ7682573.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2117..2704)
                     /locus_tag="WJL20_000377"
     CDS             complement(2117..2704)
                     /locus_tag="WJL20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ7682574.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2917..3114
                     /gene="iscX"
                     /locus_tag="WJL20_000378"
     CDS             2917..3114
                     /gene="iscX"
                     /locus_tag="WJL20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ7682575.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3182..3583)
                     /locus_tag="WJL20_000379"
     CDS             complement(3182..3583)
                     /locus_tag="WJL20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7682576.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3794..4753
                     /locus_tag="WJL20_000380"
     CDS             3794..4753
                     /locus_tag="WJL20_000380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ7682577.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQRLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4821..6131
                     /gene="tilS"
                     /locus_tag="WJL20_000381"
     CDS             4821..6131
                     /gene="tilS"
                     /locus_tag="WJL20_000381"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ7682578.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDILALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6205..6990
                     /locus_tag="WJL20_000382"
     CDS             6205..6990
                     /locus_tag="WJL20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7682579.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            7000..7197
                     /locus_tag="WJL20_000383"
     CDS             7000..7197
                     /locus_tag="WJL20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682580.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7459..7812
                     /locus_tag="WJL20_000384"
     CDS             7459..7812
                     /locus_tag="WJL20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682581.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7824..8129
                     /locus_tag="WJL20_000385"
     CDS             7824..8129
                     /locus_tag="WJL20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682582.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGADDHLDSVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8191..9567
                     /gene="mpl"
                     /locus_tag="WJL20_000386"
     CDS             8191..9567
                     /gene="mpl"
                     /locus_tag="WJL20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ7682583.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            9774..10826
                     /gene="bioB"
                     /locus_tag="WJL20_000387"
     CDS             9774..10826
                     /gene="bioB"
                     /locus_tag="WJL20_000387"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ7682584.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(10904..11539)
                     /locus_tag="WJL20_000388"
     CDS             complement(10904..11539)
                     /locus_tag="WJL20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7682585.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQIGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            11862..12122
                     /locus_tag="WJL20_000389"
     CDS             11862..12122
                     /locus_tag="WJL20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7682586.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12185..14044)
                     /gene="ilvD"
                     /locus_tag="WJL20_000390"
     CDS             complement(12185..14044)
                     /gene="ilvD"
                     /locus_tag="WJL20_000390"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ7682587.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHALTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14217..15986)
                     /gene="cysI"
                     /locus_tag="WJL20_000391"
     CDS             complement(14217..15986)
                     /gene="cysI"
                     /locus_tag="WJL20_000391"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ7682588.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRRPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(16013..17827)
                     /locus_tag="WJL20_000392"
     CDS             complement(16013..17827)
                     /locus_tag="WJL20_000392"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ7682589.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17824..19110)
                     /locus_tag="WJL20_000393"
     CDS             complement(17824..19110)
                     /locus_tag="WJL20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ7682590.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19149..20072)
                     /gene="cysD"
                     /locus_tag="WJL20_000394"
     CDS             complement(19149..20072)
                     /gene="cysD"
                     /locus_tag="WJL20_000394"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ7682591.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20110..20850)
                     /locus_tag="WJL20_000395"
     CDS             complement(20110..20850)
                     /locus_tag="WJL20_000395"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ7682592.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(20861..22303)
                     /gene="cobA"
                     /locus_tag="WJL20_000396"
     CDS             complement(20861..22303)
                     /gene="cobA"
                     /locus_tag="WJL20_000396"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7682593.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNV
                     VDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAENAPKPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQWAA"
BASE COUNT         4854 a         5831 c         6466 g         5322 t
ORIGIN      
        1 gccgccttgt cctgattttt gttaattcac tatataaaat cggtatttcg gacgtaagca
       61 attttgaagg cagaatgcgc catttggaaa acaacggcta tgtgaacgtt gctgggttgg
      121 aacgcatcct cgccgtcaaa accgacaatt acaaagaaaa agaaaacctg ctccatgaaa
      181 ttttcagcaa aagcaggata ggcgataccg aattgttcgc cgtggacgag aatcttgtaa
      241 aacgtttgtt tttatcgctt cgcggcgaaa tcgtgttccc gaaaaacgaa acggcggaat
      301 cggaatttga aaaagcgtcc acgaacgcag gcaggaaggg aatgccggat ccggccgcaa
      361 acaactgctt gatttggtac ggcgcggaca ccgggaatac ccttacgcgc tgcctcggct
      421 tttggcgggt gcggcgtcct acaagccgaa aaaatcgaaa atccgccttt ttaaagaagc
      481 atatttcggc aaaagcggca cgaggctgac cgacgaaatt gcagacggca tccatattta
      541 cacctgtttt tcgcgggcgg atttggaaaa agcctattcc gaatatttgg aatttttcaa
      601 atccgaatcg gatgccgaag gcagaaaacc acgctaaggt gcaaacagat accgtacacg
      661 ttgaggagca gtatgatggg cgattccgtc atttattatg tagaacaggc agacgaaccg
      721 gtaaaccgtg ccggcgaacg cgctcgtaaa acattcaaat acttttggcg cgaacttttt
      781 tgggaacgcc gccgcattat tcccgccctg gattttgcca tggtcaaagt cccttttttc
      841 caagacggcg aagacggcga aatttgcgaa catatgtgga tcgacgatat ttattttgac
      901 ggtctttaca tttacggtgt gctgaacaat gaacccggcg gactgaccaa tgtcgaacaa
      961 ggcgaaagcg tttgcgttcc ggttgacgac atcagcgact ggatgttcgt gtgcaacggc
     1021 attccctacg gcggctttac catacaggca atgcgtggac agatgacgga agaggagcgt
     1081 accgaacacg atgccgcatg gggaatcgat ttcggcaatc ccgggcaggt attgctggtg
     1141 tatgaagaaa aagaacatcc cgaaaatctg gaagagcatc cgatgtgccg gaactgtatt
     1201 gacgattttc ggcaacagtt gtcccaaaac tcggattatc tgcgggaaca ggacgaagac
     1261 ggctatacgc cgcttcatca tgaagccatc gcaggaaatg cacttatggt tcaagccatg
     1321 cttgaatacg gcgcaaatcc tgcctcaaag acatcggaag gctataccgc cctcgatttt
     1381 gcccgcctgg cgggctggca aaatgttgcc gacctgctcg aaccgcgaca ttaggcggac
     1441 agttttccga aaacgaacac aaacactttt tacagaaaga caataaaaat gcccaaaatc
     1501 accgtacttc cacacacgac attatgcccc gaaggcgcgg ttatcgaaga cgcgcccgaa
     1561 ggcaaaaccg tccttgacgt gctgctcgac catgatatcg aagtcgatca cgcctgcgaa
     1621 aaatcctgcg cctgcacaac ctgccacgtg attatccgca aaggtttcga cagcctggaa
     1681 gagccgaccg aattggaaga agacctgctc gatcaggctt ggggtttgga agccgattcg
     1741 cgcctgagtt gtcaggcggt tgtcgccggc gaggatttga ttgtggaaat ccccaaatac
     1801 accatcaacc acgcgcgaga agaacactga aaacaggccg cctgaagccg gcacgcttca
     1861 gacggcattg ttgcgcggat aaggcgcaat cgcccgaaaa caggcgttcg tacaggcgga
     1921 actttcgatt ctatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta
     1981 ccaatagtac ggcaaggcga ggcaacgctg taccggttta aatttaattc actatatatt
     2041 gatttttatc ggttttctga cggaacggat tggtgcggca tccgaggcgg attactcggg
     2101 cgcgatgcac gggtatttat cggttttgca gccggaaaaa ccgccggcgg gttactttca
     2161 gggcggcggt cgggcggtat gccggatagc gttccacttt cgcttctata ttgattttga
     2221 ttggttttct gacggaatga tccgatgcgg catccgggac ggcttgtgtt tcttcctgcc
     2281 cgcctgccgg atattcctgt acttgcacga aaccgaaaga ggtggcggtg cggcggacaa
     2341 gctcgagata gcgtccttca agctccggac aggcggcgga cacgctttct gccgtaaccg
     2401 tgaagccgcc gcccgacccg gcaaggcgtt ccgcaatctg cgtgtagatg cgccaacgtt
     2461 gggcgcgcaa cattgccgca tcgcaaccgg cagccgcttt atccaaggca atcagaccgc
     2521 gcctgctccg gtagtgaagg caaaccgtta ctctggagag gatatgttgc ccgtccaata
     2581 tttgatacag tttgcgtatc agcagaatca ggcggtcaaa ctcctccatc tccgacaggg
     2641 aatcaggatg gtcggaagcg gtataaacca gtttggacaa ttttgcggca aacatattgt
     2701 tcatcaatct tccttgtcgg ttgaaacccc gctcttcggg gcggtagaat cagatttgtt
     2761 tgggagggac aactcctccc agatcaggac gacacatagg ctggtgcttg atgtgttgtc
     2821 cggcgagttg aaacattcag ccatcctcaa ggtgcggcag ttttgccgaa acatattcta
     2881 cacggcttca atgccggacg ataaaaggaa attcatatga aatggaccga cacccggcgt
     2941 atcgccgaag aactctatga cctgcacggc gaaaccatcg atcccagaac cgtgcgcttt
     3001 acccaactgc gcgacctgat tatggcattg cccgaatttg acgacgaccc cgcccgttgc
     3061 ggcgaacgca tcctcgaagc cgtgcagcag gcatggatag acgaggcgga ataagtttcg
     3121 ggaatgccgt ctgaaatgcg gcggtacgcg gttcgtgctt ctgtttgcag cgggaatggt
     3181 tttaccagtc tccttttttc agcctgtcca gttggcggcg gtcgcgcttg gtcggtctgc
     3241 cgtcgggata ggcggaagtg atgcggctga attggtcgag ctgtttgcgc tcttccctca
     3301 atgttgccgt tttcgcgtcc tcttcataca gaagccgcgc ctcggatgcc gggcggcgtt
     3361 ggtggttcaa acctttaacc ttgattttat agggaaggga attgagcgtc aggtcgataa
     3421 tatcgccgat gtctatggtt ttactgtttt tgaccttcga gccgtttact tgaaccctac
     3481 ccagttcgat gtgcttttgc gcaagggaac gggtcttgaa aaaacgtgcc gcccaaagcc
     3541 atttgtccag tcgcatggcg gaagaatcgt gcttgtcttt catacgattt tgtttgaaat
     3601 aattgaattt gtttcaagtt tagcataaga tacgccgcct ataactagta tatatgcact
     3661 aatccactgt tttccatact gtccgaacac aaaaagaggg tatggaaaag ccgttttgga
     3721 caataaatta actgcggaat atgcacaaat agcgtatgat agcggcagaa tctgttgatg
     3781 agagcttcat tctatgaaac ctgttttttt ggattttgaa caacccatag ccgaactgac
     3841 caacaaaatc gatgagctgc gtttcgtcca agacgagtct gccgtcgata tttcggacga
     3901 aatacaccgt ttgcagaaaa aaagcaacga tttgaccaag tctattttca gtaaacttac
     3961 acccgcccaa atttcacagg tttcccggca tccgcagcgt ccctatactt tggattacat
     4021 tgaggcactg tttaccgatt ttgaagaact gcacggcgac cgccactttg ccgacgatta
     4081 tgcgattgtc ggcggattgg cgcgtttcaa cggacaaagc gtgatggtcg tcgggcatca
     4141 gaaagggcgc gacaccaaag aaaaaatccg ccgcaacttc ggtatgcccc gtcctgaagg
     4201 ctaccgcaaa gccctgcgcc tgatgaagac ggcagaaaaa ttcggcttgc ccgtcatgac
     4261 ctttatcgat acgccgggcg cgtatcccgg catcggcgcg gaagaacgcg ggcagtcgga
     4321 agccatcggc aaaaacctgt acgaactgac acgcctgcgc gttcctgttt tgtgtaccgt
     4381 catcggcgaa ggcggttcgg gcggtgcgtt ggcggtcgcc gtaggcgatt acgtcaatat
     4441 gctgcaatat tcgacctatt ccgttatttc ccccgaaggc tgcgcgtcta ttttgtggaa
     4501 aacggcggaa aaggcggcgg atgcggctca ggctttgggc attaccgctg accggctgca
     4561 aaggctggac ttggtcgata ccgtcatcaa agaaccattg ggcggtgcgc atcgggattt
     4621 cgggcaaacc atgaaaaacg taaaagccgt tttggaaaaa caactgcacg aagcgcaaag
     4681 catcccgctt gccgatttgc tttcgcgccg tttcgaccgc attatggctt acggcaaatt
     4741 ttcggaacaa taattcaggt agaacaagca gcaagcagtt tgtctgaaac tgcttgcttt
     4801 ttctttatcg ggacggaacc gtgctgactt tagatgcgtt tgagcaatgc ttgaaggatt
     4861 gttttcctca aggtctgaat ggaaaaaaaa cagcggtggc attaagcggc ggcttggatt
     4921 ccgtggtctt gctgcacctg cttgtccgcg ctgggaaacg ggcaggattt gtgccggagg
     4981 cattgcatat ccatcacggc ttgagccccc gtgccgacga ttgggcggat ttctgtcaaa
     5041 actattgcga tatgctaggg gtggggctgg aaacggttaa ggtctgcgtg gaaaaaaacg
     5101 gtttgggcat cgaagcggcg gcgcggcaaa agcgttatgc cgcgtttgcc gaaaaaggct
     5161 ttgacatttt ggcgttggcg caccacaggg acgatcaaat cgaaaccttt atgctggcgg
     5221 tcgcgcgcgg cggcggtttg cgcgctttgg cggctatgcc cgccgtccgc ccttttgggg
     5281 aaaaaggcat catctggcgg cctttactgc ctttttcgcg ccaagacata tgggattatg
     5341 cccaaaaaca cggtttgccg aatatcgagg acgaaagcaa tagcgatacg gcttatttgc
     5401 gaaaccgctt ccggcaccgt attttgcccg aactttcggc gcagattccc catttcgggc
     5461 ggcatgtact gaacaatgtc cgcgctttgc aggaagattt ggctttgttg gacgaaatca
     5521 tcggtcagga ctgccgttgg gtctgtgagg aaggcagttt cgatacggtg cgttggcagg
     5581 cgttttcccc acgccggaaa acccatattt tgcggtattt tttgaaggaa aacggcatcc
     5641 ccatgccgaa tcagaatgcc cttgccgaca ttgcccgggt tttgacggag gcaaaaaccg
     5701 gacgttggaa cttgcaaggc tttgaattgc atcattatgc aggcaggctg tttgtgttcc
     5761 gactggaaaa aacggataaa ctgcggtttt tgaaagacag gcagataagc ggaaatttaa
     5821 gggaaatatt gacggggcag ggatttgttt taaaacgtca tccgtttggg cttcctgagc
     5881 atcttttgga gcaggacgga attttgagga cggtagcggt atcggatacg ttggctgtcg
     5941 gcggcatcca taaggatgtg aaaaaaatcc ttcaggggaa acgggttttg cctgtcctgc
     6001 gcccaatttg gccgcttgtt gccgacagcg gaaaccgtcc attggcgttg gcaaactgtt
     6061 gtgcggattt ccaatactcg gtttcagacg gcattttgcc cgtccatcct gactttccca
     6121 ttttattttg ataatatcgc aaacagattt cggcggcgtt cagacgggta ttgtccggtt
     6181 gcatatttct aaaaggcttg tgaagtgaaa cacatcagtt cgaccaataa tgaacacatc
     6241 cggcacctgc accgcctgtt gtcgcaagga aagttcagac ggcaatacgc ccaaaccgtt
     6301 ttggagggcg tgcacctgct tcaggttttc ctgcaatccg gcagaaaacc ggtcggggta
     6361 tatattcccg agggcaaaat gccgtctgaa gaagtccgca ggctgagggc ggttgtgccg
     6421 gaaggcaagg ttttttccgt ttcagacggc atattgaaaa aaatcagcag cttgacttgt
     6481 gcggatgatg tgcttgcgct gattgatatt ccggatgcgg gtgctttgcc tgccggcggc
     6541 gactgcgtgg ttttggacgg cgtgcaagac ccgggcaatg tcggcacggt gttgcggagc
     6601 gcggcggcgg caggggtagg tacggtcgtt ttgggcaggg gttcagcgga tgtttggtcg
     6661 cccaaggtat tgcgcgcggg gatgggcgcg catttcttgt tggacattta ttcgcaggcg
     6721 gatttggaaa tatggttggc gagctataaa ggccgtgtgt ttgccaccgc cttgcgagag
     6781 gaaaagcagg cggttttgta cggcgaagat ttgtgcgaac cgacagcctg ggtgtttggc
     6841 aacgaaggcg cgggggtcgg taaagcggtt ttggacaggg cggacaagtg tgtcaggatt
     6901 ccgatgcacg atgcaaccga gtctttaaat gtcgcgatgg cggcgacaat ctgcctgttt
     6961 gaacagatgc ggcagcggtc ggcgtattga ggaaaagaaa tgccgtctga aaaaatctat
     7021 tacggcgtat tgattttctt atgtatcgct tctatgctgc tgtcgccgtt tttttatgcg
     7081 ggtgctttga agcccaagaa ggcggcattg cggaaggacg ggcagtggaa actcatctta
     7141 ttgtccaata ccgtggcggc ggcggttttg ttttggatgt ggtggaaatg gttttgacag
     7201 atattgctca aaattgggtt aatggaatcc gaacaaataa ataatttggt aaaaaatttg
     7261 ttaaatcaat ggattaaagt ttttcgggaa acaaaacagc tctaagcaaa tagggcgttt
     7321 gtcggtaaat acggaagagt tgcggcatta tcgggcatct ttaacaagta gtgccggctt
     7381 ggcaggcaat tggtttttat gggcagtttg caaaattgca gatataaaat tgcgaatcgg
     7441 ttaaagcgtg aggataccat gaaaaattgc aaattttttt atgatccgac aagggcaatc
     7501 tatgatagcg gtgcagatta cttaactagg gaaaaacata gattagtcgt tatcgcaaat
     7561 agtgcttggg ggctattgct taatttatct tgttattatg acgaggtttt ggaaaagcgg
     7621 aaaataccgt tcggcaaaca ggaaattgat gacgatatgg acaaagtgtc cgcccataag
     7681 cggaagttta aagatatttc tgaaatcaaa gtaggggatg gttgggaata cccgttcaat
     7741 tatgagcagg gaatgaaaga attagatgaa gtgctattga aatacattcc cttttttgaa
     7801 gaagaacgat aaaggaggtt gatatgcgcg tatctaaaat aattggaagt atgttgcttg
     7861 ttacagcggt tcagaccgta ttttcggcaa atgtttacgc gtgccgccat aatggtaaaa
     7921 ccagttacag ccaaactccg ggaaaacatt gtaccaacgc gggtttgggg cgggacctgg
     7981 tgtacagttc ggtcaggcct gccgtgaaag acagggcgga agacgcaggg gctgacgatc
     8041 atttggattc ggtgagggac gaacacgtcc aaaatccgag agagaatacg cataaggatg
     8101 cttcgaatac cggtgcaaag acgcattgat tgaaaccgaa tcagccatga cggcgttgaa
     8161 taatagaatt tgaacgatta gggaactctg atgaaacaca tccacattat cggtatcggc
     8221 ggcacgttta tgggtgggat tgccgccatt gccaaagaag cagggtttga agtcagcggt
     8281 tgcgatgcga agatgtatcc gccgatgagc acccagctcg aagccttggg cataggcgtg
     8341 tatgaaggct tcgacaccgc gcagttggac gaatttaaag ccgacgttta cgttatcggc
     8401 aatgtcgcca agcgcgggat ggatgtggtt gaagcgattt tgaaccgtgg gctgccttat
     8461 atttccggcc cgcaatggct ggctgaaaac gtgctgcacc atcattgggt actcggcgtg
     8521 gcggggacgc acggcaaaac gaccaccgcg tctatgctcg cgtgggtttt ggaatatgcc
     8581 ggactcgcac cgggcttcct tatcggcggc gtaccggaaa acttcagcgt ttccgcccgc
     8641 ctgccgcaaa cgccgcgcca agacccgaac agccaatcgc cgtttttcgt cattgaagcc
     8701 gacgaatacg acaccgcgtt tttcgacaaa cgctccaaat tcgtgcatta ccgtccgcgt
     8761 accgccgtgt tgaacaatct ggaattcgac cacgccgaca tcttcgccga tttgggcgcg
     8821 atacagaccc agttccacca cctcgtgcgt accgtgccgt ctgaaggcct catcgtctgc
     8881 aacggacggc agcaaagcct gcaagacact ttggacaaag gctgctggac gccggtggaa
     8941 aaattcggca cggaacacgg ctggcaggcc ggcgaagcca atgccgatgg ctcgttcgac
     9001 gtgttgcttg acggcaaaaa agccggacac gtcgcttgga gtttgatggg cggacacaac
     9061 cgcatgaacg cgctcgccgt catcgccgcc gcgcgtcatg ccggagtcga cattcagacg
     9121 gcctgcgaag ccttgagcac gtttaaaaac gtcaaacgcc gcatggaaat caaaggcacg
     9181 gcaaacggca tcaccgttta cgacgacttc gcccaccacc cgaccgccat cgaaaccacg
     9241 attcaaggtt tgcgccaacg cgtcggcggc gcgcgcatcc tcgccgtcct cgaaccgcgt
     9301 tccaacacga tgaagctggg cacgatgaag tccgccctgc ctgtaagcct caaagaagcc
     9361 gaccaagtgt tctgctacgc cggcggcgtg gactgggacg tcgccgaagc cctcgcgcct
     9421 ttgggcggca ggctgcacgt cggcaaagac ttcgatgcct tcgttgccga aatcgtgaaa
     9481 aacgccgaag taggcgacca tattttggtg atgagcaacg gcggtttcgg cggaatacac
     9541 ggaaagctgc tggaagcttt gagatagccc gggcgatgcc gtctgaaagc ccttcagacg
     9601 gcatcgcccg gctgcgcggc acaaaggcgg aaaaaccgtt tgccccgtat tttcaaacgc
     9661 gttacacttg ccgccgctgt tttcagccat ttgattaccc gcaaccgccg tcattgcgcc
     9721 ggcggtttgc ctgtcagcgt cattgcgccg ctgtaaatac gaaagaacac attatgaccg
     9781 tatcccccgt cgccttgcgc cgtaagaccg agtgcaagcc tcatcccacc gcgcgctatt
     9841 ggaaaaaatg cgatgtcgaa gccctgttcg gacttccctt cctcgacctc atttaccaag
     9901 ccgccgaaat ccaccgccaa aatttcaacc cgcgcgaaat ccagctttcc acgctgttgt
     9961 ccatcaaaac cggcggttgt cccgaagact gcgcctattg tccgcaatcg gcgcaccaca
    10021 ataccaatct gggcaaagag cagatgatgg atgtggatga aatcgtcgaa aaagccaaaa
    10081 tcgccaaatc gcgcggcgca agccggtttt gtatgggcgc ggcgtggcgc ggccctaaac
    10141 ccaaagacgt ggagacggtt tccgcaatca tcaaagccgt caagggcttg ggtatggaaa
    10201 cctgcggcac gttcggtatg ctcgaagaag gtatggcgga agacttgaaa gaggcgggct
    10261 tggattatta caaccacaac ctcgacaccg accccgaccg ctacaacgac atcatccaca
    10321 cccgccaaca cgaagaccga atggacacct tgggcaaagt ccgcaacgcc ggtttgaaag
    10381 tctgctgcgg cggcatcgtc gggatgaacg aaacccgcgc cgaacgtgcc gggctgattg
    10441 ccagtctcgc caatctcgac ccgcagcccg aaagcgtgcc gattaaccgg ttggtcaaag
    10501 tggaaggcac gccgcttgcc gatgccgaag atttggactg gacggaattt gtccgcacca
    10561 tcgccgtggc gcggattacg atgccgcaaa gttatgtccg gctgtcggca gggcgcagca
    10621 atatgccgga agcaatgcag gcgatgtgct ttatggcggg cgcgaactcg attttttacg
    10681 gcgacaagct gctgaccacg ggcaatcctg atgaggacgg cgacagaatc ctgatggaaa
    10741 agctcaacct gtatcccttg cagtttgaac cggaaggcga ggtcgccgaa gtggaaaaag
    10801 cctctgggat taaagtggat tattgacgat tgaaaaatgc cgtctgaaac ccggaaaaag
    10861 gctttcagac ggcatttgtc cggacggcat ttccaatatc tttttaccgg cgcgtgatgc
    10921 tgccgtcggg cgagacatcc agcccgttcc ccttggggaa atcgctgcgg ttcagataaa
    10981 taatccgccc gataacggtt ttctcggggt cgattttggg gattttatcg ccgatttgag
    11041 tgatggggat aatgttgccg gaaacgaacc gcccctgttt gtcggtgata attttgaaaa
    11101 tcggcgcaat gccgctgatg ccggaggtgt tgattgcgcc gtaggtggca aagttgccgc
    11161 cgctgtagga gatgaagcgg tcgcggtaaa gttcgacggc gcgagtaacg tgcgacccct
    11221 gcccgaatac gacatccgcg ccggaatcga cggcaagccg tgcaaactcg acgacgttgc
    11281 ccctgttttc cccatagaag atttcggtat cgaacggcag gtgttccgcc tgtttccctt
    11341 ccgcgccgcc gtggaacatc acaatgacga tgtcggcttt ctgtttggtt ttggtaatcc
    11401 gttttctaac tttggcataa tcgttcagtt tgacggcggc aaggttgggg gcgaaggaga
    11461 cgaagccgga tcttacgccg tttttcttca ggatggcggt ttcaaacctg ttttcgatgc
    11521 ccgaatattt gatgttcaat tcgtcaaggt tcgcccttta tgccgtttcc gcaccgcaaa
    11581 ccgccggggt caagccctcg gcgcatcctg ccggaacgga atccccgtgc tgccgattga
    11641 cggttcaaac cccgcgcccg tttcaaatac cggcgatgtg gacggacagg atgcgcctga
    11701 cgaaaagaca gccgataccg tttccattat cggcgtgggc gacattatgc tcggcagcaa
    11761 ttatccggtc gattacctgc ccgataccaa tattctgaaa aacgtcgaat ctgccttgca
    11821 agacgcggac attaccgtcg gcaacctcga aggcacgctg tttgacgaag gcggtacgcc
    11881 gaaaaaatgt gcaaaccccc aaaatatgct atgcattccg aacgccctcc gcatacgggc
    11941 aataccttgc cgacgcggga ttcgactacc tcagcttcgc caacaaccac agcaacgact
    12001 tcggcgcgca aggcatcacg gcaacggcgg cgagcggcag ctcttttaca tactcgatcg
    12061 cgctaaagcc gctgccgata acgaggccaa aattgcggaa aataccgcca tcgcccagat
    12121 aaatttgtcc atcatcagac ctttactgtt cagacgagac agcatttgcc gcacgttttg
    12181 gggcttatct ttcgatttgc gctacgtcgc gcaccgcgcc tttgtcggcg gaagtcgcca
    12241 tcgcgccgta agctcttaat gcggcggaga cgtagcggtc gcggttttta ggcttccatg
    12301 ctttgctgcc gcgcgcttcc atttcggcac ggcgtgcggc aagctcttcg tcggaaatgg
    12361 caaggtggat gctgcggttg gggatgtcga tttcgacggt atcgccttcg tgtaccaaac
    12421 cgatcgcgcc accttccgcc gcttcgggcg aggcgtgtcc gatggacaaa cctgatgtgc
    12481 cgccggagaa gcgtccgtcg gttaagagag cgcaggcttt gccgaggcct ttagatttca
    12541 ggtaggaagt cggatacagc atttcctgca tgcccgggcc gcctttcggg ccttcgtagc
    12601 ggatgatgac gatgtcgccg gcgacgattt ggttgcccaa aatgccttct actgcgtctt
    12661 cttggctttc aaacacgcgg gcgcggccgg tgaatttgag gatgctctca tccacgcctg
    12721 cggtttttac cacgcagccg cgttcggcga tgttgccgaa caagaccgcc aaaccgccgt
    12781 cttgcgagta ggcgtgtgcc acgtcgcgga tacagccttt ttcgcggtcg aggtcgaggg
    12841 ttttccacat acggttttgc gagaacgctt gggtggtgcg tacgccgccg ggcgcggctt
    12901 tgaagcgttc gatggcgcgg gtgttttcag ggttggttac gtcccatttt tcaatcgcgt
    12961 ctttcagggt cagcgcgtga atggtgtaca cgtcggtgtg cagtttgccc gctttgtcca
    13021 attctttcag gatggcgaag atgccgccgg ctcggtgcac gtcttccata tagtagtcgt
    13081 ggttgttggg cgcggttttg cagatgcaag gcacaacgcg gctcaggcgg tcgatgtccg
    13141 ccattttgaa atcgacaccg gcttcgttgg caacggccaa caggtgcaaa atggtattgg
    13201 tagagccgcc catggcgatg tccatggtca tggcgttttc aaacgctttt ttggtggcga
    13261 tgctgcgcgg cagtacggtt tcgtcgtctt gctcgtaata gcgtttggtg atttcgacaa
    13321 tcatacggcc tgcttcgagg aacaattctt tgcggccggc gtgggtcgcc aaatacgaac
    13381 cgttgccggg cagggaaagg ccgagtgctt cggtcaggca gttcatcgag tttgccgtaa
    13441 acatacccga acacgagccg caggtcgggc aggcgttttg ttcgacttcc tcgacttgcc
    13501 ggttgctgac attgtcgtcc gccgattcga tcatcgcgtc aatcaagtcc aaacggcgtt
    13561 cgggttggat gtttgccacg ccgataactt tgcccgcttc catcgggccg ccggaaacga
    13621 agatggtcgg gatgttcagg cgcatcgcgg caatcagcat gcccggggtg attttgtcgc
    13681 agttggaaat gcacaccagc gcgtcggcgc agtgggcatt gaccatatat tcgatggagt
    13741 cggcaatcaa atcgcggctg ggcagggaat acagcatacc gctgtgtccc atagcgatgc
    13801 cgtcgtcgat ggcgatggtg ttgaattctt tggcgatggc gcccgccttt tcgatctcgc
    13861 gggcaaccag ctggcccata ttgtgcaggt ggacatgacc tggcacgaat tgggtgaacg
    13921 agttggcaac ggcgataatc ggtttgccga agtcggtttc catcacgccg gtggcgcgcc
    13981 acaacgcgcg cgcgcccgcc atattgcgac cgtgggtgga ggttttggag cggtattctg
    14041 gcatagtgtg tttccttgtg cctataccgt ctgaaagaca gggctgtttc agacggtatc
    14101 gggtacggtt ttttagagtg ggaaaagagg gtattttata ccaagtatcg gaattttgcg
    14161 ggattgaaac ggcgtgcggc aaaaaagaaa atccccgcag gaatgcgggg acgggttcag
    14221 gcgcgggcaa tcgcgacggc tttggatgcg tcccaaaaat caacgggtgc atttaatacg
    14281 ggtttgacga tgcccgtccg tatggcgaaa tcgccgaaac cttcgccgat attgcgttct
    14341 gccgcccatt tgccgatcag gtcgtccaat tcggcaagga tttcgggcag ggtgatgttt
    14401 tctttgtaaa gacgggggat gcgtacgcct tcacggtcgc cgccgatatg gaggttgtag
    14461 cgtccgacgg ctttgccgac cagtccgatt tccgccaaca tcgcccgtcc gcagccgttc
    14521 gggcagccgg taatgcgggt aacgatgtag tcgtccgacg tgccgtgttt cgccataatc
    14581 ttatccagct cgccgatgaa gtccggcagc acgcgttcgg cttccgccat tgccagcggg
    14641 caggtcggga aggaaacgca cgacatcgca ttttcgcgca gtttgctgac accgtcgtgt
    14701 atcagcccgt atgagcgggc gagctgttcg atccgggctt tgcctccttc gggtacattt
    14761 gccacgatga ggttttggtt ggcggtgatg cggaagtcgc ctttgtggat tttggcgatt
    14821 tccaacacgc cggtcagaag ctgtttcccg ccttcgtcaa ccaaacgccc gctttcgatg
    14881 aaaagggtca aatgccagtt gccgtctatg cctttcaccc agccgatgcg gtcgccgcgc
    14941 ccggtaaatt tgaacgggcg tatgggttcg aacggcatac ccatacggcg ttcgacttcc
    15001 gcgcggaagt tgtccaagcc catattttga atggtgtagc gggtgcgggc gtttttgcgg
    15061 tcgctgcggt tgccgaagtc gcgctgtgtg gttaccaccg cttcggcggc cttcagcgcg
    15121 tgttccggag gcacgaaacc cagttccagt gaaatgttcg gataggtttt ggtgttgccg
    15181 tgttccatcg aaagcccgcc gcccgccaaa acattgaatc cggcaagctg tccgttgccg
    15241 tctgaaatgg cgacaaaatc caagtcgttg ccgtagcagt ccacatcatt taagggcggg
    15301 atgacgactg cggttttgaa tttgcgcggc agataggttt tgcccaaaat cggctcgtct
    15361 tcttgaagga agtcgtcgga actttgaact tttttgccgt ccacccacac atccagataa
    15421 ccgcgcgtgc gcggcagcag gtgttcggaa atctttttcg cgtattcgta agcctgccgg
    15481 tgcagttcgg actcgatcgg gttggacgtg caaagcacgt tgcggttcat atccgccgcc
    15541 gtggcgatgg aatccaaacc cagtttgtgc aagaggcggt gcatcgtctg caacttggct
    15601 ttcggcacgc cgtgaaattg gaaggtttgc cggttggtca gccggatgga gcggtaatga
    15661 ctgttttccc gggcaaattt gtccagttct atccattggg acggtttgat gatcccgccc
    15721 ggccgccggc agcgcaaaag cataaatttc aagggctcga gttttgcctc ggcgcgttcg
    15781 gcgcggatgt cgcggtcgtc ctgctcatac ataccgtgga agcggatgag ttggaagttg
    15841 tcgcctttga agccgcccgt gagcgggtct ttcaaatcgt ccaaaatcgt gccgcgtaaa
    15901 aaattgcttt cggttttcag acgttcgttg tcggatagcg gtttttcttg ccacgccaaa
    15961 ccttttgtct tggtctgtac ggtcattttg tgttcctccc gattatattt aatcaataaa
    16021 catcacgctg atagcgtttt tcttcgcgca gcatatccaa atattcttct gcgccctctt
    16081 cgtccaaatg tcctgccccg ataatcacat ccagcaaggc ggcttccacg tcttttgcca
    16141 tttttgccgc atcgccgcac acatagatat gcgcgccttc ctgcagccat tgccaaagtc
    16201 cttccgcctg ttcgcggatt ttgtcctgca catagatttt ttcttcctga tcgcgggacc
    16261 aggcgaaatc gtacctgtgc aggaagccgt ctttggcaaa ctgctgccat tcggtttgat
    16321 agagaaaatc acgggcaaaa tgcggattgc cgaaaatcag ccagtttttg ccttccgcat
    16381 tttctgcggc acgttgttgg acgaaggcgc ggaacggtgc gacgccggtg cccgagccga
    16441 tcatcacaat cggcttgcgg ctgtcttcgg gcagcctgaa gccgtcgttg cgttccacaa
    16501 acacgcgcac cgtgccgtcc tcttccagcc ggtcggcaag gaaacccgat gcgccgcccg
    16561 ccctggcgcg cccttcgtgt tcaaaacgca ccgcgccgac ggtcaggtgc acttcgtccc
    16621 ccacttccgc ctgcgacgag gaaatcgaat acaggcgcgg cgcaagtggg cgcagcaggc
    16681 cggcgaattg ttccgccgtc agttttgccg ggaagcggtg cagcacatcg gcaatcggcg
    16741 tgctttgcac aaagccttgc aaaacggcgt tgtcggcagc aatacggtcg agttcgtcat
    16801 catcggcgaa cggggcatag cctttgacaa aggcgggggt gttttgcgtg agttcaaaat
    16861 gggataacag tgcggaggca accggcaggg tttttccgcc cgcctgtatt tccgttgccg
    16921 gatcgatgcc gagcaggtct aggatttccc tgaccagtgc cggatcgttg tcaaaccaaa
    16981 cgccgagcgc gtcgcccggg aggtagtgca aatccgaacc gctcaaatcg atttcgatgt
    17041 ggcgcacgtc tttatcggat tggcgggcgg tgattttctg attggccagc agggcggcgg
    17101 gaaaggggtc tgccttgcag tacctgccct ccgatgccgt ctgaaggccg gcggggggcg
    17161 ttgcctgcgg cgcgggcgtt gcccggtttt ttgcggcttc ttcttttaag agtgcggcga
    17221 tattgtctgt ccaggcgttt gcggcggcgg caaagtccaa atccgcatca acgcgttcga
    17281 gcaggcgttt cgcccccaat tcttcaaaac gtttgtcgaa atctttgccc gcccggcaga
    17341 aattcggata ggaactgtcg cccaaaccca gtacggcaaa ttggagtttg tccaatttcg
    17401 gggctttttt gccgttcagc agtttgtgca gcacgacggc ttcttccggc ggttcgcctt
    17461 cgccttgggt ggacgtaacc agcagcaggc ggcgttcgcc ggcgatgttt ttcgccttat
    17521 agtctttcag ttcggcgcga ctgacttgga tgccggcggc ttccaggctg tccgccgctt
    17581 tgtcggcaac ggatttcgca ttgccggttt gcgaggcgga aaggacggtt acggaaaaag
    17641 gttctgccgt cggcaatgcc gtctgaagcg cgggcagtcc tgcagatgcc ccgtttcctg
    17701 cttttgccca agcgtagccg gacagccacg cccattgtgc cgcgtccagc cccgacagga
    17761 gctgcgtgat ttcgggcggc agaggcggta atggcggatt tgtgttctgc atatcgtgtt
    17821 cactcataaa atcatacctg ccgcaacagt gccgtatgtc gcttcgtcta tcaggataaa
    17881 cgaaccggcg gcggtgtttt ccgcataagg cgttgccgta acgggttttt gaaggttgat
    17941 gcggactttg gcgatgtcgt tcatcttcaa ggattccgcg ccggcctctt gttccagcgt
    18001 gcggacatcc aaaacgcttt caatttcccc gacttttgcc ggcacggttt gcgtgccgtg
    18061 cttgagcagg tatttgcgcg cggtgttgag cggacgttcg tcaaaccagc aaagcgtggc
    18121 ttccagatgt ttttgcgggg cgagcgggga atttttatcg acaaaaaggt cgccgcgcga
    18181 aacatcgatg tcgcggtcca gccggagggt tgccgcctcg ccggcaaaag cctgcgccac
    18241 ttcccctttc ggcgtgatga tttcggacac ttcggcggtc agcccgttcg gttcgatgcg
    18301 gacggtttgc ccgacggcga ccgaaccgcg ttcgatgcgc ccctgatagc ctcggaaatc
    18361 atcggccttg tcggcatctt ggcggacgac cagttgcacg gggaaataaa aatcgtcggc
    18421 ggtgcggctg acttcgtccg cccccggcag ggtttccaaa atggacaata agggttcgcc
    18481 tttataccaa ggcatattgc cgccggggta aacaatgttg tcgcccaaga gtgcggacac
    18541 cggtacgaaa tgcacgtctt tcaaaccgag ctgttcggca agtcggcggt atgcctccac
    18601 aatggcgttg aatttgtctt cgctgtaatc cagcaggtcc attttgttga ccgccaccac
    18661 aatatgcggg cagtcgagtt ggcggaggat ggcggaatgg cgtttggtct gcggcagaag
    18721 ctgcaagggc tgcgcgccga aatccagttg ggatgcgtca accagcacga ctgccgccga
    18781 agcggtgctt gcgcccgtaa ccatattgcg cgtgtattgt tcgtgccccg gcgtgtcggc
    18841 gatgatgaat ttccgtttcg ccgtggaaaa atagcggtat gccacatcga tcgtaatgcc
    18901 ctgttcgcgt tcggcttcca gtccgtcggt caggatggag aagtctatgg tttctttcaa
    18961 acctttgctt ttgccggatt ccaaggtttt gatttggtcg gacagcaggg ctttgctgtc
    19021 gtagagcagt cgtccgatta gggtgctttt gccgtcatcg acgctgccgg cggtaatgaa
    19081 gcggagcggg gtttggtgtt gtgccgtcat attttcttcc tcatatctgc ttaaagggtt
    19141 tttgaaattt agaaatagcc ttcttttttg cgtttttcca ttgccgcctc gcttgcctga
    19201 tcgtccagcc gggtcgcgct gcgttcggaa atgtcggcaa ccgctgtttc tctgataatc
    19261 tccgtcggcg tggacgcggt gctttctacc gggcaggtgc agctgatgtc gccgacggtg
    19321 cggaagcgga catcaaggat ttcggaggtt tcagacggca ttttcggggt gagcggcgtt
    19381 acagggacca gcagccccct gcgtctgacc acttcgcgcc tgtggctgta ataaatcggc
    19441 ggcagctcga ggttttcgcg ggcgatgtat tgccagatgt cgagttccgt ccagttggaa
    19501 atcgggaaga cgcgcatatt ttcgcctttg tgcagcctgg tgttgtacag cgaccacagc
    19561 tcggggcgtt gcgccttcgg atcccattgt ccgaactcgt ctcggaacga gaaaatccgt
    19621 tctttggcgc gggctttttc ttcgtcgcgc cgcgcgccgc ccatcagcgc gtcaaagccg
    19681 tttgcctcga tggtttccaa cagcgtaacc gcctgcgccg cattgcgcga atcggtttct
    19741 ttgcgtaaaa ctactgtgcc tttggcaatg gagtcttcca cgcgccccac tatcaggcgg
    19801 gcattgagtt ttgccgcctg cgcgtcgcgg aaagcaatca cttcggggta gttgtgcccc
    19861 gtgtcgatat gcaccagcgg gaagggcagt tttaccggcc tgctgcccag ccggaaggct
    19921 ttgcaggcga gggcgagcag gaccacggaa tctttgccgc cggaaaagag cagggcgggg
    19981 ttttcgcatt ccgccgccac ttcgcggatg atgtggatgg attcggattc caaccagtcg
    20041 agttgggcgt tgttcggttc ggttttcgtc ataccatatt ccttatttct tctgtctgat
    20101 atttatgaat tatttgtgca gcccgcattc tttgctgttt ctgccttccc accaccaccg
    20161 cccggcgcgg atgtcttcgc ccgccttgac ggggcgggtg caggggtcgc agcctatgct
    20221 gggaaatcct tgccggtaca aatcgttgta aggcacattg ttggcgagga tgtatgccca
    20281 cacgtcgtgt tccgaccagt cgaaaatcgg gttgtatttg ccgatgcccc gtccggcatc
    20341 gtattcggca aacggcagtt ccgtgcgtgt ggcggattgt tcgcggcgtt gcccggtaag
    20401 ccaggcgtcc gcgcctgcaa tggcgcggtt gagcggttcg atttttcgga tgcggcagca
    20461 ttcgcggcgc gcttcaacgc tgtcgtaaaa ggcaaacctg cctttgcttt ccacataacg
    20521 gtcggcatct tctcgaaccg gccggaaacg ctttatccgc aaatggggat atgcgcgtcc
    20581 gagcctgtcc agcaggttca gggtttccgt gtggagcaga cccgtatcca aggtaaaaat
    20641 gccgatattg aggttttcgc cggcgataag gtcggtaatc accatatctt ctgccgcaag
    20701 gctgctggca aaccgtgcat cccggtggct gccggcaatc cggtgcaggc gttgtttgag
    20761 ggtttccgtt ttttccgcaa gggcggtttc gccgccggat ccgatatgcg gtatctgcca
    20821 cagggcgggt ttgaacagtg tcgtttccat ttttcccgcc ttatgccgcc cattgtccgg
    20881 cattcagtcc gcccaatgcg ggatacgcct gcccgactcg gttttctcct tcgccgtttt
    20941 caccgaacca ggcgagtttt tcgtgcagcc ccaccacttc gcctatgaca atcaatgccg
    21001 gcttcggcgc gttttcggcg agttcggcaa ggttggcgag cgtgccggtt gcggtttttt
    21061 gagccggcag cgtgccttgg ctgataacgg ctgccggcgt gtcgggcgag cgtccgtgct
    21121 gttgcagccg ttcggcaatc agggcggctt tgagcgcacc catataaatc accaaggtct
    21181 ggcggctgcg ggcgagggtc tgccattcga tgtcgggcgc atccgccttg cggtggccgg
    21241 ttacgaaaac cgcactttgg gcataatcgc ggtgcgtgag cgggatgccg gcataggcgg
    21301 tcgcgccgac ggcggcagta atgccgggga cgaccgaaaa cggaatctga tggcgtgcca
    21361 aggtttccaa ttcttcgccg ccgcgtccga acacgaaagg gtctccgcct ttcaaacgca
    21421 ccacgcgcct gccttcgcgg gccagcctga ccataagcgc attggtgtcc tcttgcgggg
    21481 tgcggccgcc ccgggcgcgc ttgccgacaa aaatccgttc cgcatcgcgg cggacgaggg
    21541 acagtatgcc gtctgaaacc agcgcgtcgt aaagcaccac gtctgcctgc tggatttcct
    21601 gtagcccttt gagcgtcagc agccccgcat cgccgggacc cgcaccgacc aaagacacaa
    21661 aaccgccttg ataattttgt tccaactgct ttgccaactc ctgttcggct tgggcggttt
    21721 gttgattttt aacgagggcg gcaaaacggc cgttgaactg cttttcccaa aaacggcggc
    21781 gttcggtaac ggatttcagt ttggctttga ccgcatcgcg ccatcttccg gagatttccg
    21841 ccatatcgcc caaagaatga ggcagcaggg cttccagttt ttcacgcagc aaacgggcga
    21901 ggacgggcgc gctgcccgaa ctggaaacgg caatttgaat cggattgcga ttgataatcg
    21961 aaggaaaaat aaaactgcaa taatcgcggt cgtccaccac gttgaccggt ttttggcagc
    22021 tttcggcaag ctggaaaacg cgccggttga gggcttggtc gctgcttgcc gcaatgatga
    22081 ggaaaactgt gcggatgtgt tcggcacgaa attcttcggc aagccacagg attttgtttt
    22141 ctgccgccag cgcacttaat tcagcattca actgatttgc aacaaccctg acttgcgcgc
    22201 cggcatccag caacaggctg attttgcgcg cggcgaccga accgccgcct acgaccaata
    22261 cggggcggcc ggcgaggttg gcgaaaatgg ggaaataatt cactggcgaa ctcctttgct
    22321 gtttgcccgc accttgtttc cgatacggtg cgtcgcggca tttttgtcgg aatgcgggtc
    22381 attttagaca aaaggatttt cccggttaaa taataaaaag gcatttgtta gaagctgaaa
    22441 gctatatgag ggcggctgcg gatgcggcgg ttt
//
LOCUS       DAWKQJ010000013        22436 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000013 DAWKQJ010000000
VERSION     DAWKQJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.013"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..380)
                     /locus_tag="WJL20_000397"
     CDS             complement(<1..380)
                     /locus_tag="WJL20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682594.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WJL20_000398"
     CDS             complement(438..803)
                     /locus_tag="WJL20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682595.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1333)
                     /locus_tag="WJL20_000399"
     CDS             complement(785..1333)
                     /locus_tag="WJL20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682596.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
     gene            complement(1496..1768)
                     /locus_tag="WJL20_000400"
     CDS             complement(1496..1768)
                     /locus_tag="WJL20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682597.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2330..2803)
                     /locus_tag="WJL20_000401"
     CDS             complement(2330..2803)
                     /locus_tag="WJL20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682598.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(2891..3115)
                     /locus_tag="WJL20_000402"
     CDS             complement(2891..3115)
                     /locus_tag="WJL20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682599.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(3386..3673)
                     /locus_tag="WJL20_000403"
     CDS             complement(3386..3673)
                     /locus_tag="WJL20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682600.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIRKTINKIK"
     gene            complement(3985..4356)
                     /locus_tag="WJL20_000404"
     CDS             complement(3985..4356)
                     /locus_tag="WJL20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682601.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITLEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(4492..4935)
                     /locus_tag="WJL20_000405"
     CDS             complement(4492..4935)
                     /locus_tag="WJL20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682602.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(4978..5847)
                     /locus_tag="WJL20_000406"
     CDS             complement(4978..5847)
                     /locus_tag="WJL20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ7682603.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            6011..6934
                     /locus_tag="WJL20_000407"
     CDS             6011..6934
                     /locus_tag="WJL20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7682604.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     SNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(7012..7653)
                     /locus_tag="WJL20_000408"
     CDS             complement(7012..7653)
                     /locus_tag="WJL20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7682605.1"
                     /translation="MQKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCIAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(7708..8499)
                     /locus_tag="WJL20_000409"
     CDS             complement(7708..8499)
                     /locus_tag="WJL20_000409"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ7682606.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLNVFEGRVLELPAKSHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFHL"
     gene            8665..8740
                     /locus_tag="WJL20_000410"
     tRNA            8665..8740
                     /locus_tag="WJL20_000410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8698..8700,aa:Thr,seq:tgt)
     gene            8882..9673
                     /gene="panB"
                     /locus_tag="WJL20_000411"
     CDS             8882..9673
                     /gene="panB"
                     /locus_tag="WJL20_000411"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ7682607.1"
                     /translation="MITVNTLQKMKAVGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            9796..10632
                     /locus_tag="WJL20_000412"
     CDS             9796..10632
                     /locus_tag="WJL20_000412"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ7682608.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            10797..12635
                     /locus_tag="WJL20_000413"
     CDS             10797..12635
                     /locus_tag="WJL20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682609.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLEKAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            12775..13356
                     /locus_tag="WJL20_000414"
     CDS             12775..13356
                     /locus_tag="WJL20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ7682610.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            13366..14211
                     /gene="ispE"
                     /locus_tag="WJL20_000415"
     CDS             13366..14211
                     /gene="ispE"
                     /locus_tag="WJL20_000415"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ7682611.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            14219..14294
                     /locus_tag="WJL20_000416"
     tRNA            14219..14294
                     /locus_tag="WJL20_000416"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14252..14254,aa:Gln,seq:ttg)
     gene            14307..14382
                     /locus_tag="WJL20_000417"
     tRNA            14307..14382
                     /locus_tag="WJL20_000417"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14340..14342,aa:Gln,seq:ttg)
     gene            14391..14466
                     /locus_tag="WJL20_000418"
     tRNA            14391..14466
                     /locus_tag="WJL20_000418"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14424..14426,aa:Gln,seq:ttg)
     gene            14634..15617
                     /locus_tag="WJL20_000419"
     CDS             14634..15617
                     /locus_tag="WJL20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ7682612.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            15684..16256
                     /locus_tag="WJL20_000420"
     CDS             15684..16256
                     /locus_tag="WJL20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ7682613.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(16381..17550)
                     /locus_tag="WJL20_000421"
     CDS             complement(16381..17550)
                     /locus_tag="WJL20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ7682614.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            17698..19224
                     /gene="ilvA"
                     /locus_tag="WJL20_000422"
     CDS             17698..19224
                     /gene="ilvA"
                     /locus_tag="WJL20_000422"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ7682615.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(19281..20354)
                     /locus_tag="WJL20_000423"
     CDS             complement(19281..20354)
                     /locus_tag="WJL20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ7682616.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(20351..21211)
                     /gene="cysW"
                     /locus_tag="WJL20_000424"
     CDS             complement(20351..21211)
                     /gene="cysW"
                     /locus_tag="WJL20_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ7682617.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(21463..22299)
                     /gene="cysT"
                     /locus_tag="WJL20_000425"
     CDS             complement(21463..22299)
                     /gene="cysT"
                     /locus_tag="WJL20_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ7682618.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         5630 a         5145 c         5807 g         5854 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcaccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccccctcag tctccttcat ctccttctga gaaagctccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccatcgcc ggagcggcaa tcattgtcag aatcatcatc gctgcggtaa
     1321 tttgtttttt cataataact cctttggatt acaaggttgg aaaatcaaag ccctgcttag
     1381 aacgtatgtt gcacacccaa tttcaatagg taaatcagat tgcaaatcca gcaatttgaa
     1441 tattgtcatt gttccgtgca aaagggattt ttattgatga gttgtgtact gggtttcagc
     1501 ttggcttttt agataatctt ttcacaagaa catacatcaa tactgtccaa aaaaaaagat
     1561 ttgataaata tattgcaaca tattttattc cacctccatc cgaaacagaa accaatatat
     1621 ttttaattaa tacatagccg attattacat ttattattcc tataatatat aaggatcttg
     1681 ccaaaatttt ttttgatttt attttaggaa tatctcttat ccatgctaaa atacagccca
     1741 atgtcgaaaa gaaaataaca gcaaacacta ccttacctcc tctcacttcc aaccaaaccg
     1801 atagcaggtt tggttggaag ttggtttatt tattatttaa cggcgaatgt cagtgttctt
     1861 acccgtagaa cctgcataac cagttatgcc accagcaaca gtccccactg ctacacctcg
     1921 cataccatta atcagaattc ttcctatttt ttcgaatcca ctatttcttc caaagatgca
     1981 gcaaacgcag gttgagccta gacccaatct acgtttgctg ccgcatctga tccctattgt
     2041 ttcttatcct tacatcttcc tgccttgtca atcaaataaa gacagaagac tatacaaaaa
     2101 ctgaccgaca tactgaaaat acctatcccc ttccatgcaa tctccgcacc attgacccaa
     2161 tagatcagac tgagaaacaa caaagccaca gtataaatca acgtaaaaaa tattgcgtaa
     2221 agcacaataa agggaacttt tatctcacgg ttgtttttta taatatattc agcaacttga
     2281 tttccgaata tacctgataa aaaatatagt attagatcat ccatttcgtt tatcttctat
     2341 gttttcccat tgcggcgcta gaagactgat ttaatgctgc accatttgca cgaataactg
     2401 cattaggaat agcatttccc cctttccaag cagaacgggc aaaaacacta gtagtaatcc
     2461 ctgcaccgcg caacatcgca ctgctatatc cgcctcctat cataccacca gcggttgcag
     2521 ccaaggtact tcttgttgaa gcaaattgcc ctgttttaat tttagaaatg ccgtgattag
     2581 cccaagcact aattgcccct cccatcaaag cacctgccac aataggaaga aacgccccct
     2641 cagtctcctt catctccttt tgagaaagct ccgccaactg catcggcgca tctgcctgcg
     2701 tgtggaatac gtatttttca aatgcctgat tgtccaatcc gtttgccatt gcgggggcga
     2761 ttatagacag catcatcgtg gcttcagtga tttgtttttt cataacaaat tcttagggag
     2821 gagataatgt tccatcaaaa tcctcctgaa aggctgcctg aaatttccaa gcagcctttt
     2881 tgattgctta ttaatcggat agtgcgacaa atataaagtg ggcggcaatc attgccgtaa
     2941 cggcagccat aactgtttta ttttcttcaa accaaagcaa gttgagaaac ataaacatca
     3001 agaaatatga tggttcttca gttttgccgc tgctttcttg agttagaaga taaaatatag
     3061 atatggcggt tatgccgatt gctgctttta ttaaaatata tttgtttttc atcatcttgt
     3121 atggtttttc atgcaagttt caacaataaa attcggaata agatagagag atgttgatat
     3181 ttcagataaa tatacaaaac aatcaagaaa ctattggcgg tatgtataat cttgaatttc
     3241 tttttttgat gaaattaaaa taatgaaata tatggtaaac gttacaacgg aagccttagc
     3301 ctcccgattg cagtatttca attccaagat aaatgccgca tagaaaagat taaatactta
     3361 aatggttttt agcggcttaa atatcttatt taattttatt gattgttttc ctaatagttt
     3421 ctgcaataaa taataagaaa tagaataata aacaaacaga acatgaaaat aaaaataaat
     3481 ctttcattat cccagtaata aaactccaaa gattttcctt atttccatca acaataaaaa
     3541 ttatcagttc atgaataaag aatactgcaa agaaaatgct tcctgccgtt agagatattt
     3601 ctaatctgct tattttccct tcgctatatt tacctgataa atagcctaaa aaagctgcaa
     3661 tcaaatcatc cattttaaaa atttctgtaa aaacaggtaa tgtctttaat tcattacctg
     3721 ttttgccatt atttatagtg gattaaattt aaatcaggac aaggcgacga agccgcagac
     3781 agtacaaata gtacggcaag gcgaggcaac gcaacgccgt actaatccac tatatatatc
     3841 cattcatata gagaaaaatt cttataccca atataaaaca aggtctttca ttatattttg
     3901 aaaaacctag aagctttgta tctattggtt tatcaaaatt aataattatt gcagaagaca
     3961 tgtaggctaa taatcttgat ttatctatat tttattaatt atatatttaa ccccgaagtc
     4021 aatatagaaa tttagtgatt ttaaagttct cttatcaatt ttaatctcag ggagtacata
     4081 aaaatcctca gaataggcaa actctatata tgcatcatca ttcgataata attcaataat
     4141 atttttcatt tcatccgaaa gaaatgtaac catctcatat tgacatttat ttttagatac
     4201 catttttgaa tattgattaa ataattttgg attactattt ttagtaatta atccttccag
     4261 agtaataaaa ccactttgta taaagtcatc acttatatta acagccttcc gaaaaaaaat
     4321 ataatcacac tctttgttat caatataaaa tctcatactc tttaataatg aaatataaga
     4381 atttccagaa ttacaagcaa tttgaaaaaa catcattact cctaaaactc ataaatagta
     4441 aggaatatat atatatatgc atattcctta ctattttttc acaaattatg cttatctatg
     4501 cttatttcca taaaaagcat gccctcgaat tttttgactt gagttctgga aatgagaata
     4561 atttgaatgg atttccttct gtccatgtct aataacattt tgtcttcccc aagcttggtt
     4621 tgctaccgct cttgcttgtt gagatgttcg tgcatagaca ttagctcctc ttgataaagc
     4681 ttgtgctgca acacgctgcg taacccaacg ctgcgcaagg aatctgcccg catgcaaaat
     4741 tcctattgcc accaacggtg ccgcagcccc ttcagtttct ttcatctcct tttgagaaag
     4801 ctccgccaac tgcatcggcg catctgcctg cgtgtggaac acttggtttt caaatgtctg
     4861 attgtccaag ccgtttgcca ttgcgggggc aatcatagac agcatcatta cggctgaggt
     4921 gatttgtttt ttcataataa ctcctttgga ttacaaggtt ggaaaatcaa aacctgctta
     4981 aaatgtatgc tgtacgccaa atttcagttc ggaactgctt tgccctgaaa cgttgaaacg
     5041 tgcggatgcg tttaaagccg tggttttggt gaaaccgaaa cctgtgccga aatgagcgta
     5101 ggtggatgtg tttcttgcgg tttctttttt gccgtctatg cggtcgggct gtgtgcccag
     5161 ccattggatg cctccggtca ggctgattct gtcgttggcg gcaaatgaga tgttggggtt
     5221 gagcagcagg tagttgcccg atttgtagcg gatgccgtct gaaagggttt tgctgccgtt
     5281 gatgcggtag gcggcggtga gggaaaggac aatcggatct atggctttgt aggtggtggc
     5341 gccgatgagc caggattttc ccgacgaggc tttgttgcgc gatttttcgt aaaccgtgct
     5401 ttcaagaaag ctgattaggg cggggttttt gtcgtcttta aggaaagtgt ggctgatgcc
     5461 gagggatacg tcggacatcc gtttgttgcg ggttttgccg ttgccgtcga gtttgcgttc
     5521 ttcgtgccac agatagctgc cgctgccgta aatgtcggta ttcccggtca gtccgtagcg
     5581 caaaccgagc gtgccgacga gcatatcggt attgctgccg ttttcttgga tttcggtcgg
     5641 aatggggata aacgaggttg cgccggtttg aatgtaaacc ggtgcggcaa gttcggcgcg
     5701 gttgttttcg ctgttcaggt aggtaaggga agtttccagt ttccattttc ccttgtcggt
     5761 cattatgtct tcaatcgtca agggcaggtc ggcataagtg gataaaggca ggatggcggg
     5821 caaggcgggc aaaaagatgc gcttcatatt tctcctattg atattaattc ttatttactg
     5881 ataataatga atatccgata atcttgtcta aataaatttt taaatcataa aaaacaatat
     5941 gtttgttttt gtcaattatg tttcgatatg ccgtctgaaa agtttgtgaa aaacggttac
     6001 aatccgccgc atgaaaaaac gcaataatcc tcttccgctg ttgaacggtg tcaaacccag
     6061 ttatttggtg ctgccgcatg aaaagcagtt ttacgggctg ccgctgctgc attttctgtg
     6121 catccgcttt ccttttgtgg gcgcggacga ttggcgcagg cggttgaaca gcggttttgt
     6181 ggtcggttcg gatggtgcgg cgttggacga acatactttg ttcgagccgg gtaaggtgat
     6241 gttttattat cgggaaacca gtcgcgagag cgagccgcgt attccgtttg aagaaaagat
     6301 tttgcatatt gatgaacatt tgattgtggt ggacaagccg cattttctgc ccgtcatccc
     6361 cagcgggcgt tttttgaggg aaacgctgct cacacgtctg cgcctgcgtc ctgaattgca
     6421 gcatttgaat gttgaagaca ttacgccgct gcaccgcttg gacaaggata cggcaggcgt
     6481 gatgctgctg tcgcacaatc ctgccacgcg gggggcttat cagacgatgt ttcaaaacaa
     6541 aacggtatgg aaaacgtatg aggcgcttgc cccgacaagg acggatttgc cgtatccgct
     6601 tgatgtggtt tcgcgtttgg taaggggtga aaaatttttt acgacgcaag aggcggaagg
     6661 cgaatcaaat gcacatacga cggttgaact aattgaaaac aggggggaat tcagccttta
     6721 ccgccttacg ccgcataccg gcaagaaaca ccagttgcgc gtacacatga tgggcttggg
     6781 tatgccgtta ttgaatgacg cgctctatcc cgtgccgtct gaagcgggca gcgaggatta
     6841 tcggaaacct ttgaagctat tggcaaaaaa gattgcgttt gcagatcctt tgtcgggtcg
     6901 ggaaagggtg ttttgcagcg gattttgcct ataatgagag ggaacgcaaa cccgtatcgg
     6961 cggatgccgc cgatacgggt tttacattat cgatgccgtc tgaaaggggt tttatccgtt
     7021 caggcatacg ggcgcgaagc ggaggatggt gtcgcgcagg acgatgagtt tgccgtgaaa
     7081 gaaatgcgtg gaaccggcga tggtaatgac gggcaaatct tgcggttccg cccatttcag
     7141 tgctttcccg atttcgacga cttcgtcttc cgcgccgtgt atcatcagcg ttttggcaac
     7201 attgggaacg gcggacggct cggggcggcc ggtatagtgg cagactgccg cgccgatgag
     7261 cagcaataaa tcgggtatgc gcgcttgtgc ggcaaatgtg gcgacataac cgccgaagga
     7321 gaagccggat aaggcaaatt cgggggcttc gggatgttgg gcgcgggcat agtcgatgac
     7381 ggcgatacag tcttgcgttt caccgcgtcc gtaatcgtgt gtgccttcgc tgccgcctac
     7441 gccgcgaagg ttgggcaggt agcagtggaa gccgagtttg cttaaggctt tggcggcggt
     7501 ttggatgact ttgttggtgt tcgtcccgcc ttggaggggg ttggggtggt tgatgacggc
     7561 aacaccgcgt gccggtactt gttcggacgg gatatggatg gtttctaaaa tgccggcagg
     7621 gccgggtatg tgtatggttt caggtttttg cataatggtt tcctcttgat tttccgcata
     7681 ttccgatgca gccgcgaaga tggcggctta aaggtggaaa tgcttgccgt tgttggggtt
     7741 ggatgctttc aggtcggcga gcatgcggtg gaagtccaat ccgtaagcgt tgctcagttt
     7801 gtcggcacgt tttttgagtt tgtctatgtt ttgctctttg gggctgcttt ggaatgccat
     7861 taccgcgaca ttgccgtgac ttttggcagg aagttccaat acgcgccctt caaagacgtt
     7921 taacaggcgt tcgatgaagc gttgatagcg tttgtcgccg ctccaccagt tggtaacgaa
     7981 tatgccgtct gaagagagtg cgttgcggca gtctcggaag aacggttctt caaccagcgt
     8041 atcgatgatt tgttcgccgt cgaatccgtc caccaagatg atatcggtgt tgtggcggaa
     8101 gactttgatg tactctgcac catctgcttc aataatttca aatttttcgc cctcgaaagg
     8161 caactcgaac aggttgcggg caatggcgat aacctgcggg ttgatatcta cggcagtttg
     8221 acgcgtatcg ggcaggtagg tatctatcca gcgtgcaaac gagccgccgc ccaagccgat
     8281 ttgggtgata tgttgcggaa gagcgtcggt aaacagcagc cagcccatca tcgcgcggct
     8341 gtaagagagc accagctcgg acgggtggtc gaggttcatc gagctttgaa cggtgtcgct
     8401 gcccaagtga agcgaacgga tattgccttc ttcggaaatg ccgacttcgg gaaagccgga
     8461 ttttgcagga cgcaggcggc ggtagggatg tcttgccatc ggtgtgaacg gtcggtttga
     8521 aaggcggata ttttatcaga atgccgatgc ttgttctgtt tcaaattaat ttcttttcaa
     8581 ataaattact tattcggatt tgccggggct ttcggataaa ttccttgcca aggtgcggca
     8641 ttgcctgcat aattcgcttt ctttgccggt atagctcagt tggtagagca cctgacttgt
     8701 aatcaggggg tcccgagttc gactcttggt gccggcacca gatttgacag cccgattgtg
     8761 taaaacagtc gggctgtttt gcatttgcgg cacggtcggt ggcagggctg cgggcatttc
     8821 actataatag cccgtttcaa actgaaaagg ccgtctgaaa agggcggggt aacaatatct
     8881 gatgattact gtgaacacac tgcaaaaaat gaaggcggta ggtgagaaaa tcgcgatgct
     8941 gaccgcttac gaatccagtt ttgccgcgct gatggacgat gccggcgtgg aaatgctgct
     9001 ggtcggcgac tctttgggta tggcggttca agggcggcag tcgacgctgc ccgtcagcct
     9061 gcgcgatatg tgctatcaca ccgaatgtgt agcacgcggt gcaaaaaatg cgatgattgt
     9121 cagtgatttg ccgtttggtg catatcagca gagtaaggag caggcgtttg ccgccgccgc
     9181 cgaactgatg gcggcaggag cgcatatggt taaacttgaa ggcggtgtgt ggatggcgga
     9241 aacgactgaa tttttgcaaa tgcgcggtat tccggtttgt gcgcacatcg gtctgacccc
     9301 gcaatccgtg tttgccttcg gcggatataa agttcagggg cgcggcggca aggcgcaggc
     9361 gttgcttaac gatgccaagg cgcatgacga tgcgggggcg gcggtcgtgc tgatggagtg
     9421 cgtaccggcg gaactggcaa aaaaggtaac tgaaactgtt tcctgtccga ccatcggcat
     9481 cggcgcgggt gcggattgcg acgggcaggt tttggtgatg cacgatatgc tcggcatttt
     9541 cccgggtaag acggcgaaat tcgtcaaaaa ctttatgcag gggcatgata gtgttcaagc
     9601 ggcggttcgg gcgtatgttg ccgaagtcaa agccaaaact ttccctgctg cggaacatat
     9661 ttttgcagat tgaacggttt acatgcagca tgccgtctga aagccgtttc agacggcatt
     9721 ttgttttgcc gtgcgcggcg cgtataatcg gcgcgttttg tcgggcagga agcccgaagg
     9781 ataaggatta ccgttatgca aatcatacat accattcgag aactgcgcgc gtggcgtaaa
     9841 aatgcgggaa aggtggcatt tgtgccgacc atgggcaatc tgcatgaagg gcatcttgcg
     9901 cttgtgcgtg aggcgaaaaa acgcgcggac agtgtcgtgg tcagcatttt cgtcaaccgc
     9961 ctgcaattcg gtcagggcga ggatttcgac aaatatccgc gtactttgca acaggatgcg
    10021 gacaagctgg aagccgaggg tgttgccgtt gttttcgcgc ccgatgagaa agaactctat
    10081 ccgaacgtgg aacagcgtta caacgtcgaa ccgcccaatc tgcaaaatga gttgtgcggc
    10141 aagtttcgtc cgggacattt ccgcggggtg gcaacggtag tgtccaagct gttcaatatt
    10201 gtcgcgccgg atgttgcctg tttcggcaaa aaagactatc agcagcttgc cgtgattaaa
    10261 ggtttgaccg aggatttgaa ttttgatgtt gaaatagtgc ctgtcgatac ggggcgcgcg
    10321 gaagacgggt tggcactctc cagccgcaac cagtatttga gtgcggcgga acgcgacgaa
    10381 gcaccgcgcc tgtaccgcga attaaaggct gttgccgaat ccttggcgca gggcagtttg
    10441 gattatgcag gtttggaaaa acgtgccgtc caatccctga cagaatacgg ctgggtggtc
    10501 gattatgtcg aaatccgccg cgtcgatacg ctcgaagtgg cgcgggcggg agataagaaa
    10561 ctggtggtct tggccgccgc ccgtctgggg acgacgcgcc tgattgacaa tttggaaata
    10621 aaactccctt aaaccgcaag cgtcgggaat gccgtctgaa gcggatttgc gtttcagacg
    10681 gcatttattt ttttgaacgg ggtttcgcaa atctacaaac gattctgctt gtgataaagt
    10741 tacgcctgat tatatgccgt ctgaaggttc ggacggctgt cggataaagg aagattatgt
    10801 tacccgcccg tttcaccatt ttatctgtgc tcgcggcagc cctgcttgcc gggcaggcgt
    10861 atgccgccgg cgcggcggat gcgaagccgc cgaaggaagt cgtaaaggtt ttcagaaagc
    10921 agcagcgtta cagcgaggaa gaaatcaaaa acgaacgcgc acggcttgcg gcagtgggcg
    10981 agcgggttaa tcagatattt acgttgctgg gaggggaaac cgccttgcaa aaggggcagg
    11041 cgggaacggc tttggcaacc tatatgctga tgttggaacg cacaaaatcc cccgaagtcg
    11101 ccgaacgcgc cttggaaatg gccgtgtcgc tgaacgcgtt tgaacaggcg gaaatgattt
    11161 atcagaaatg gcggcagatt gagcctatac cgggtaaggc gcaaaaacgg gcggggtggc
    11221 tgcggaacgt gctgagggaa agaggaaatc agcatctgga cggactggaa gaagtgctgg
    11281 ctcaggcgga cgaaggacag aaccgcaggg tgtttttatt gttggcacaa gccgccgtgc
    11341 aacaggacgg gttggcgcaa aaagcatcga aagcggttcg ccgtgcggcg ttgaaatatg
    11401 aacatctgcc cgaagcggcg gttgccgatg tggtgttcag cgtacagggg cgtgaaaagg
    11461 aaaaggcaat cggagctttg cagcgtttgg cgaagctcga tacggaaata ttgcccccca
    11521 ctttaatgac gttgcgtctg actgcacgca aatatcccga aatactcgac ggctttttcg
    11581 agcagacaga cacccaaaac ctttcggccg tctggcagga aatggaaatt atgaatctgg
    11641 tttccctgca caggctggat gatgcctatg cgcgtttgaa cgtgctgttg gaacgcaatc
    11701 cgaatgcaga cctgtatatt caggcagcga tattggcggc aaaccgaaaa gaaggtgctt
    11761 ccgttatcga cggctacgcc gaaaaggcat acggcagggg gacggaggaa cagcggagca
    11821 gggcggcgct aacggcggcg atgatgtatg ccgaccgaag ggattacacc aaagtcaggc
    11881 agtggttgaa aaaagtgtcc gcgccggaat acctgttcga caaaggtgtg ctggcggctg
    11941 cggcggctgt cgagttggac ggcggcaggg cggctttgcg gcagatcggc agggtgcgga
    12001 aacttcccga acagcagggg cggtatttta cggcagacaa tttgtccaaa atacagatgc
    12061 tcgccctgtc gaagctgccc gataaacggg aggctttgag ggggttggac aagattatcg
    12121 aaaaaccgcc tgccggcagt aatacagagt tacaggcaga ggcattggta cagcggtcag
    12181 ttgtttacga tcggcttggc aagcggaaaa aaatgatttc agatcttgaa aaggcgttca
    12241 ggcttgcacc cgataacgct cagattatga ataatctggg ctacagcctg ctttccgatt
    12301 ccaaacgttt ggacgaaggc ttcgccctgc ttcagacggc ataccaaatc aacccggacg
    12361 ataccgctgt caacgacagc ataggctggg cgtattacct gaaaggcgac gcggaaagcg
    12421 cgctgccgta tctgcggtat tcgtttgaaa acgaccccga gcccgaagtt gccgcccatt
    12481 tgggcgaagt gttgtgggca ttgggcgaac gcgatcaggc ggttgacgta tggacgcagg
    12541 cggcacacct tacgggagac aagaaaatat ggcgggaaac gctcaaacgt cacggcatcg
    12601 cattgcccca accttcccgc aaaccccgga aataatgcag gtccatcctt tcagacggca
    12661 taaggtttgc cgggaagctg cggcattcgg gcaaaccgca cgcagttcgc acgcgttttg
    12721 cacggcacgc cgaacccatc ggccggcagg atggcatccg ttaaggaaat tctgatgaaa
    12781 cataccgtat ccgcatcggt catcctgctt ttgaccgctt gcgcgcaatt gcctcaaaat
    12841 aacgaaaacc tgtggcagcc gtccgaacac acccgcagtt tcacggcgga agggcggttg
    12901 gcagtgaaag cggaagggaa aggttcgtat gcaaatttcg attggacata ccaaccgccc
    12961 gtggaaacca tcaatatcaa tacccctttg ggcagtacgc tcgggcagtt gtgtcaagac
    13021 agggacggcg cattggcagt ggacggcaaa ggaaatgtct atcaggcgga aagtgcggaa
    13081 gaattgagca ggcagctggt cggtttcaaa ctgccaatcc aatatctgca tatctgggca
    13141 gatggcaggc gtgtggcggg cgcgccttac cgcatcctgc cggacggcat attggaacaa
    13201 tacggttgga ctgtcggcag aaccgccgac agtggggggc aagtccgaac gttgcaactg
    13261 aataacggaa atttgaacat caggctggtt ttcaccgaaa tcggtatgcc gtctgaaact
    13321 gaaaccccgg aacgctgtgc ggcgcgcacg agataaggcg gacagatgaa tattgcggac
    13381 ggacggcagg cgtttcccgc acctgcaaaa ctgaatctcg atttgaggat tactggaaag
    13441 cgggaagacg gttatcacaa tatcgaaagc atattctgcc tgatagattt gcaggatacc
    13501 gtatatttga aactaaggga cgacggcaaa atcatcctgc acaatccggt tgatggcatg
    13561 ccgcaggaag tagatttaag ctaccgtgcc gcatcgttgc tgcaaaaata tgcgcgcaac
    13621 cccgccggcg tggaaatatg gctggacaaa aaaatcccga caggggcggg tttgggcggc
    13681 ggaagctcgg atgcggcaac cgttttgctg gtgttgaacc gttggtggca gtgcggtctg
    13741 acgcagcggc agctcattga ttcgggcgcg gctctggggg cggacgtacc gttttttatt
    13801 ttcggcaaaa atgcgtttgc gcggggtata ggcgacaggc tggacgaaat ggatattccg
    13861 aaacagtggt atgtcatcgt caaaccgccc gtccacgttt ccactgcaaa aattttcaca
    13921 cacgaaggct tgacacgaaa ttccgcctca agcataatgc cgactttcca aaacctgcaa
    13981 ccgtttagaa atgatatgca ggcagttgta tttaaagaat accctgaagt ttggaaagcc
    14041 tattccgaat tgtcccgata tggatttgcc ttaatgacag gttctggtgc gtgtgtattc
    14101 acggcgtgtc aagataggaa tagcgcatac aatatatacc gacaagtttc agatttgtac
    14161 gaggcatatt tggcagaggg tctttcaaaa catccttggt tgtccgtata aacattgttg
    14221 gggagtcgtc aagcggttaa gacactggat tttgattcca gcatgcgaag gttcgaatcc
    14281 ttcctcccca gccaagccaa acgagttggg gagtcgtcaa gcggttaaga cactggattt
    14341 tgattccagc atgcgaaggt tcgaatcctt cctccccagc cagccctttt tggggagtcg
    14401 tcaagcggtt aagacactgg attttgattc cagcatgcga aggttcgaat ccttcctccc
    14461 cagccagata aaagcgtgta agcctgctta cacgcttttt atttcataga aataaaaata
    14521 ttgaaatgcc tttgtttgtg tcggatgttg caggtataat gtcgggcttg gtacaagcag
    14581 agggaagcat tgtgttttct gagcggaagt taaacataaa atcaggtgag aatatggctg
    14641 cgtacgacag tttgatggta tttacaggca atgccaatcc cgaattggca caacgtgttg
    14701 tcaggcattt ggacatttct ttgggcaatg cttccgtatc caagttttca gacggcgaag
    14761 ttgccgtcga actgttggaa aacgtacgcg ggcgcgatgt tttcatcctt cagccgacct
    14821 gtgcgccgac caatgacaac ctgatggaaa tcctgacgat ggcggatgca ctgaagcgtg
    14881 cttcggcagg tcgtattacc acagccattc cgtatttcgg ctatgcgcgc caagaccgcc
    14941 gtccgcgttc cgtccgcgtt ccgatttctg ccaaactggt ggcaaatatg ctgtattcgg
    15001 cagggatcga ccgtgttttg actgtcgatt tgcatgccga ccagattcaa ggtttcttcg
    15061 atattccggt ggacaatatt tatgccaccc cgattctgtt gaacgacatc aaacaacagc
    15121 ggattgaaaa tctgaccgtc gtcagcccgg acatcggcgg tgtcgtccgc gcccgcgccg
    15181 tggcaaaatc cctgaatgcc gacttggcaa tcatcgacaa acgccgcccg aaagccaatg
    15241 tggcggaagt catgaacatc atcggcgata ttcaaggtag aacctgtctg attgtggacg
    15301 atatgattga cactgcaaat acgctgtgca aagccgccgt cgccctgaaa gagcgggggg
    15361 ctgaacgtgt tctagcctat gccagccacg ccgtattctc cggagaggcg gtcagccgta
    15421 tcgcctcatc cgaaatcgac caggtggtcg taaccgatac cattcctttg tctgaagcgg
    15481 ctaaaaactg cgaccgtatc cgtcaggtaa cgattgccgg tctgttggcc gaaaccgtcc
    15541 gccgcattag caatgaagaa tccgtctcat atcttttcaa tgaagaagtg atgacaggca
    15601 gcatgttgct gccataagcc cgaagccgtc ttaagctggt cgcggccgat gacggcgatt
    15661 ttacctaact tggagtattt aacatgactt atgaaattca agcctctgtt cgtgaagcac
    15721 aaggcactgg tgcgagccgc cgcctgcgtc gcgaaggcca aatccccggc attctgtacg
    15781 gtgaaggtca agagcctgtt gcaatcgctg tggatcacaa aaccgtattc tacgcattgg
    15841 aaaaagaatc tttccatact gcgttgatta agttgtctct gaacggtgaa accaaagacg
    15901 ttatcgtccg tgatttccaa atgcacccgt tccgccgcga agttcaacac atcgacttcc
    15961 aagctgtgaa agccgatcaa cttgtacgca tccgtgttcc cctgcacatc gttaacgctg
    16021 aaaattccca agcggtcaaa ctgcaaggcg gccgcgtatc tctgttaaac acttctgttg
    16081 aagtagttgc tttgcctgcc aacatccctg ctttcttgga tttggattgt gctgaagtgg
    16141 ttgccggcga cattctgcac ttgtcagaca tcaaactgcc tgaaggtgta gaaagcgttt
    16201 ccctgaaacg taacgaaaat ctggctgttg ctaccgttac cggtaagaaa cgctaattga
    16261 tttcagcagc agggcggcgc gtatgcaata cgtaccgccc tgttgtttta tgccgtctga
    16321 accgtgtttc agacggcatt tctttatttg ttggaaaaac gggatatttg aaacggcaga
    16381 ttactgccct gtcagacacg cccaaagcct ttgccaccgg cttctttttt ttacattttc
    16441 cagtgcgacg atttcttttt cggcaatggt gtatccgttt tgtctgattt tgatttttcc
    16501 taaaatttgc ccttttttta ctggggcggg aatcggctgt atggtttcta gaatttgttc
    16561 tgccattttc gcttccttat gtggcagagt gatgtaggct tctttgagga agcctgcgcg
    16621 gacggttttt ttgctgcctc cggaaatttg gatttgggca acggttttgc ctttcggata
    16681 tattttgggc gtatcgaagg cctgcaatgc ccagttcagc agcttgctgt tgtctgatgc
    16741 gcgtgtttcc gccgattccg aacccaatgt gatgacaagg atgtgcctgc cgttgccgga
    16801 gtatgacacg gcaaggttgt agccgccgct ttctgtgtgt ccggctttca gaccgtttac
    16861 attgttgtcc ctatataaaa ggatattgcg gttgttttgt tctatatttt tgaatttgaa
    16921 agatttgatg gaaaacagcg ggtaatattc cggaaagtcg cgcatcaatg cttcagacag
    16981 cagggcgagg tctttggcgg tggaaacctg tccttctcta ctcaagcctg tcgggttttt
    17041 gaatacagtg ttcttcatgc ccaagcgtcg ggcttctttg ttcatttgtt gcacaaaatt
    17101 ttcaatcgag ccgttgccca gccggccggc aagggttagg gcggcatcgt ttgcggatag
    17161 tgcaatcatg ccttttaaga gtttgtcggt gctgaccgta tcgccgggac gtacaaacat
    17221 tctgcttcct tctgaagccc atgcggattc gggtattttt aagttttctt cagattggat
    17281 atttcccgat ttcatgtttt tgaaaaccaa atatgcggtc atcagttggg ttagtgccgc
    17341 cggttcgaca ggggtattga tgtttttggc ggataaagtc tgtctgcttt gaaggtcgat
    17401 aacgatgtgt gccgctgtga gggtttcggg tgtttggaac gtgggggcgg cgtgtaccgc
    17461 cggtttgttg ggcgcgggcg atgcggccgt tgcgtgagaa acgcctaaga tgatggaaag
    17521 caggacgggc aggattttat gtgctgtcat gaaatattct aattgtgtgc gtgtttcagt
    17581 ctgccgatta tacgcttagg gtgtctgatc gggcggattt ttcttgcttt cgcgccgtct
    17641 tgggcgtatg gttttgggtt ttgcgatttt aataaaccga ttatcccata ttgaattatg
    17701 aacacgcccc ttccttattc caattacctc atccgcatcc tgacggcatc tgtctatgat
    17761 gtcgcggtcg aaacgccttt ggaaccggca cgcagccttt ctgtacgttt gaaaaacaac
    17821 atccttttga aacgcgaaga tttgcagccg gttttttcgt tcaaaatacg cggcgcgtac
    17881 aacaaaatgt ccaagttgcc gaaagatgcg ctcgcttgcg gcgtgattgc ggcaagcgcg
    17941 ggcaatcatg ctcaaggcgt ggcattgtcc gcacagcgtt tgggctgccg tgccgttatc
    18001 gttatgccgg agactacgcc gaaaatcaaa gtggatgcgg ttaagagccg tggcggcgag
    18061 gtggttttgc ggggcgtttc atacaacgat gcctacgatt atgcgatgga gttggcggaa
    18121 aaagaagggt taacctatat cgcgccgttt gatgatcctg atgtgattgc gggacagggg
    18181 acggtgggga tggaaattgt cagccagcat cccgatccaa tccgcgccgt attcgtaccg
    18241 ataggcggtg gcggtttggc ggcgggcgtg gcggcattta tcaagcaggt ccgtcccgaa
    18301 atcaaagtta tcggcgttca gaccaacgat tcctgctgta tgaagcagtc ggtcgaagcg
    18361 ggtgaaatcg tccatttgaa agatgtcggg ctgttttcag acggcactgc ggtcaaagtc
    18421 gtcggaaacg aaaccttccg cctctgcaaa gaacttttgg atgaaatcat tacagtcgat
    18481 accgatgcgg tttgcggcgc ggtcaaggat attttcgatg acacgcgcag cattaccgag
    18541 ccggcgggcg cgttggcgtt ggcgggtctg aaagcctata tcgcccgaga aggcgcggaa
    18601 aaccaaaccc tgattgccgt taccagcggt gcgaatatga attttcaccg tttgcgccat
    18661 gtgtcggagc gtagtgaatt gggcgagggc aacgaaggta tttttgccgt taccatcccc
    18721 gaagaacgcg gcagcttcct taagtttgtc aatatattgg gaaataggaa tattaccgag
    18781 ttcaactacc gctacggaga cgatgaaaaa gcgcatatct ttgtcggact tcaagcggca
    18841 ggtccgcagg atttggcggt tatcggtagc cggttggatg aggcgggatt gcccaatgtc
    18901 gatttgacca acaatgagat tgccaaaatc catatccgct atatggtcgg agggcggacg
    18961 gacaaagtag aaaacgagcg tctggtcagt tttgagtttc cggagcgtcc gggggcgttg
    19021 gcgcgttttt tgaaccatat gcagggcggt tggaacatta cgctcttcca ttaccgcaac
    19081 cacggtgcgg attacgggcg gattttggtc ggtatcgatg tgccgccgca cgatgccgcc
    19141 gcatttgacg gtttcttgga aagtctggga tacagctatc acgaggaaac gcaaaatgcc
    19201 gcgtacaagc tgtttcttgc ctgacgcttg aaagcacaat gccgtctgaa agcctttcag
    19261 acggcattgc gctttcatgg ttaaatcgaa tattcaatca gttcgttttg agagaagaca
    19321 taaacctgtt tcggaatcag cttcaattct ttgccttcgg cgattgggta acgcgcggca
    19381 tcgctgccag ccagcgtgat atgcacgtct tgtttgccgt gtttcaccaa aatatgcgtc
    19441 aatgccccga cggcgtggac tttttcgatt tcggcacaaa tcatcggtgt ttcgtgttcg
    19501 gcggcgatct gccattcgtg cgggcggata tagccggtgg cggtttgttc ctgccatttg
    19561 tattgtgcgt ccaatttcca cgcgaagccg ttataatgcc agaagccttt ttcgatacgt
    19621 ccttcaaaag cgtcggtttc gccgaggaac tcggtaacga aggcattttc gggtttgcgg
    19681 taaatagctt cggcgctgcc ggtttgttcg attttgccgt ggttcatcac gacgatttcg
    19741 tcggaaactt cgagggcttc ttcttggtcg tgcgtaacca gaatgctggt tacacccagg
    19801 ttgtgatgga tgtcgcgcag ccaggtgcgt aattctttgc gtactttggc gtccaacgcg
    19861 ccgaagggtt cgtccaaaag caggagtttc ggttcgaccg caagcgcacg ggcgagggcg
    19921 atgcgctggc gttgcccgcc ggagagttgg tgcggatagg attttgccaa atgcgagagt
    19981 tgtacgagtt tcagcaattc ttcgacttta gtgcagattt gttctttgga cgggcgttcg
    20041 gacttgggca atacggtcaa accgaaagcg acgttgtcaa acacgttcat gtggcggaag
    20101 agggcgtagt gttggaacac gaagccgact ttgcgctcgc ggacgtgttt ggcggttacg
    20161 tcttgcccat caaacagaat cttgccgccg tcggcgtttt ccagtccggc gataatgcgt
    20221 aaaagcgtgg ttttgccgca gccggacggg ccgagcaggg aaacgagttt gccggtgggg
    20281 acgttgaggt tgatgttttt cagcgcgtga aaattgccga agtgtttgtt taagttttgg
    20341 atggtgatac tcatactgcg ttcctttcgg cggcggcgag ttttttgtct tgtaatttgg
    20401 tgatgatgtt ctgcaccgcc agcgtcgcca gtgccaaaag tgccaatacg ccggagaggg
    20461 cgaatgcgcc ggtgaagttg tattcgttgt agaagatttc gaccaaaagc gggacggtgt
    20521 tggtttcgcc gcgtatgtgt cccgatacca cgctgaccgc gccgaactcg cccatcgcgc
    20581 gggcgttggt gaggatgatg ccgtagagta acgcccattt gatgttgggc agggtaacgc
    20641 gccaaaacat ctgccagccg cttgcgccga gtatcaatgc cgcctgttct tcgctgtcgc
    20701 cctgagcctg catcagcggg atgatttcgc gcgcgacaaa ggggaaggta acgaacagcg
    20761 tcgccagaat aatgccgggg atggcgaaga taatctgtat gccttgcgct tcgagccagc
    20821 cacccaatgc cgtgtgcgcg ccgaacaata agacgaacat taaaccggcc accacgggcg
    20881 atacggaaaa cggcaaatcg agcagggtag tcagcaactg cttgccgcga aaatcaaaac
    20941 gggtcagcag ccacgccatc gccacgccca agacggcatt gacggggacg acaatcagcg
    21001 cggtaatcag cgtcaatttg atggcagacc acgcttcggg atcgtttaag gatttcaggt
    21061 acaaatccca accgcctttt aaggcttcgt aaaatacggc gacgagcggc acgaccagca
    21121 tcagcagcag aaagcccagc gcggcggcaa tcagcaacac gcgcagccga cgcggttcgg
    21181 tcaggttggg attggcggaa tagggtttca tggcggtggt gttctctggt tggttttaaa
    21241 atgtttggag gtcgtctgaa aaactgtgtt tttgggagcg gattcgtttt cagacaacct
    21301 tttattgatt aagggaataa aaacgtctag ctaccgtcat tcccgcgcag gcgggaatcc
    21361 accacagggc aacaggaaaa cagaaaataa ataaggcagc cgaaattcac caatggattc
    21421 ccgcctgcgc gggaatgacg gtaacaggta tttcagacga cctcaaccct tcgcgcccga
    21481 acgcctgccc aacgcccact gcatcacgtt cagcgcaaac agaatcacaa acgaaaccag
    21541 cagcataaac aacgccaccg ccgacgcgcc ctgtacgtcg aactgttcca gcttgcccgt
    21601 aataatcagc ggcaggattt cagaaaccat cggaatattg cccgcgataa aaatcaccga
    21661 gccgtattcc cccgttgccc gcgcgaacat cattcccgcg ccagtcaaga gtgccggcgt
    21721 gatttccggc agcagcacgc ggcggaacgt cgtaaagcgg tttgcgccca aagtcgccgc
    21781 cgcttcctca tattcgcccg ataattcctc caacaccggc tgcacggcgc ggacgataaa
    21841 gggcaggctg acgacgacca gcgcaatcca aatgccgacg ggcgtaaacg cgattttgat
    21901 gcccaaaggc tcgaaaaaac ggcctatcca accgttgggc gcatacaggg tcgccaacgc
    21961 gatacccgta accgccgtcg gcagcgcaaa cggcaaatcg accagcgcgt tcgccagacc
    22021 cttgcccggg aattcataac gcaccaatac ccacgccacc agcgtgccga acacgacatt
    22081 ggtcagcatc gcataaaacg acatccgcaa actcagccag acggccgcca agacattggg
    22141 ttcggcaatc gtgttccaaa agccgcccca gccgatttcc gccgccttcg ccgccatcat
    22201 cgcaaacggc aagaccacaa gcagcgacag gcacaatacg gtcagaccaa ggctgagttt
    22261 gaagccgggc agtacgccgg gcgttttgag cggtaacata aaacaatgct gaaaataagg
    22321 aaaaggaagg actactttaa cgatgccgtc cgaaaaacgg aaagaatgga aagtttggtg
    22381 caaagacgaa tttgttataa agcggttggc agtttctcaa gcgggcgcga tgtttt
//
LOCUS       DAWKQJ010000014        22329 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000014 DAWKQJ010000000
VERSION     DAWKQJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.014"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1034)
                     /locus_tag="WJL20_000426"
     CDS             complement(120..1034)
                     /locus_tag="WJL20_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7682619.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WJL20_000427"
     CDS             1290..2759
                     /locus_tag="WJL20_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ7682620.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WJL20_000428"
     CDS             3011..3382
                     /locus_tag="WJL20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682621.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WJL20_000429"
     CDS             3455..4864
                     /locus_tag="WJL20_000429"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ7682622.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRNPL
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDAIIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WJL20_000430"
     CDS             4910..5704
                     /locus_tag="WJL20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682623.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WJL20_000431"
     CDS             5892..6668
                     /locus_tag="WJL20_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7682624.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="WJL20_000432"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="WJL20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7429..9900)
                     /locus_tag="WJL20_000433"
     CDS             complement(7429..9900)
                     /locus_tag="WJL20_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ7682625.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNLRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="WJL20_000434"
     CDS             complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="WJL20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ7682626.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIATVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(11080..12360)
                     /locus_tag="WJL20_000435"
     CDS             complement(11080..12360)
                     /locus_tag="WJL20_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ7682627.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLMLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12513..13238)
                     /locus_tag="WJL20_000436"
     CDS             complement(12513..13238)
                     /locus_tag="WJL20_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ7682628.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13311..13988)
                     /gene="radC"
                     /locus_tag="WJL20_000437"
     CDS             complement(13311..13988)
                     /gene="radC"
                     /locus_tag="WJL20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ7682629.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="WJL20_000438"
     CDS             complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="WJL20_000438"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ7682630.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15720..17129
                     /gene="leuC"
                     /locus_tag="WJL20_000439"
     CDS             15720..17129
                     /gene="leuC"
                     /locus_tag="WJL20_000439"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ7682631.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17226..17480
                     /locus_tag="WJL20_000440"
     CDS             17226..17480
                     /locus_tag="WJL20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682632.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17542..18183
                     /gene="leuD"
                     /locus_tag="WJL20_000441"
     CDS             17542..18183
                     /gene="leuD"
                     /locus_tag="WJL20_000441"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ7682633.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18352..19623
                     /locus_tag="WJL20_000442"
     CDS             18352..19623
                     /locus_tag="WJL20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7682634.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19639..20370
                     /locus_tag="WJL20_000443"
     CDS             19639..20370
                     /locus_tag="WJL20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7682635.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20404..21474
                     /gene="leuB"
                     /locus_tag="WJL20_000444"
     CDS             20404..21474
                     /gene="leuB"
                     /locus_tag="WJL20_000444"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ7682636.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21637..22200
                     /locus_tag="WJL20_000445"
     CDS             21637..22200
                     /locus_tag="WJL20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ7682637.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5396 a         5860 c         5808 g         5265 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 aattttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgggaagcc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaatcat gcgcttgttc gttacggata ttccggagga
     3661 cgatttgcgc gatattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggt aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcagatgta cagcttgcaa gacgagaata tccacaatat
     4021 cgccgtgaag gggatgtttg acgactgtca ggacattgtg aaggcggtgc agaatgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acagatgggt ttgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gagcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt ttgacctgat
     4441 ggatcgtaat cctctggaaa tcaatacgct gtgggcggaa gtggcggcgg gaaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgatcatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ctatactgct ggacgaactg gcggcaaaac aggaggcagc
     4981 catcgctccc cacctgcttg ccgacggaac caaggtatgg gtacgcaaag caggcaggca
     5041 caatgcgcgg tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg caggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga ggtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcagatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggtccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc tcctttcttg agcatcacca aagaccccga aatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggtcg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccgcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgt gcgtttgtag
     6661 atcaataagc tgtttgaaaa tcccgtctga acggcaccag caatcaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaatcaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaggcggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatggatt
     6961 cccccctacg cgagactgac gacggtgttc tgcagtagcc aaagcagaaa accatcctta
     7021 ttgtcatttc atcatgatat aacaataagg atgttaaaaa ttagttatca ttatatgcat
     7081 gatatataag aaaaattgtc ccaataagaa atgttatgca gaaaataaaa tgaaaaggat
     7141 ggttttcatt gaaaatggtt gttctgccac cgtcatacac agaaccggta tttggaaaaa
     7201 aaataatcca tattgacatc attattccat ttatcgcata aatccacttt ttaatttttt
     7261 tcctcattaa tacaatctct tatttaataa aatcaaatac ctagcaattc gatctcaaat
     7321 tttgaacgcc aacgtgtttg agcggtaaca atacatactt taagtttgca attcttcatc
     7381 gtcttatttt tgttttcatc aactatacgt ctaaagcaac gccgtccgtc attgttcgga
     7441 cggcattttt tcagactgca ttttcccccg tttgtgaagg cgcagggcgt tgcccaaaac
     7501 cgccagcgaa ctgaagctca tacccagtgc ggctatccac ggttggacat agcccaaaac
     7561 ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt gccggataat
     7621 atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat cttcgttcaa
     7681 taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg cggcggatac
     7741 gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca gcactttttt
     7801 cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg cttgggcgcg
     7861 gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc ggtcgccgct
     7921 gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg ccgcgctgtc
     7981 tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc cgaggtaaac
     8041 cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga cataggatgc
     8101 cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc ccacgccttc
     8161 gccgatgcgg ttgaggcgtt gtttaatagc gatgtcgggg acgctgccgt ctgaaatgcg
     8221 gcagttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag cctgcgccac
     8281 cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga cggcgggttt
     8341 gccttgggtc agcgtgccgg ttttgtcgaa gatgatgtcg gtggtttggg cgagggtttc
     8401 gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc agcgtaccgg tagaagctgc
     8461 cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca gcagggcgac
     8521 ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga agacggggac
     8581 ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca actcggcagt
     8641 gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca gtcgcgtgcc
     8701 accgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg tgccggcggt
     8761 tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca tagattcgtt
     8821 gacggcactg cttccttcca gcaccgtgcc gtcaacgggg atggtttcgc ccggtttgac
     8881 cagtaccaca tcgcctgctt ggagcttgac gacggcggct tcgcggacat cctgtacggc
     8941 aggatagccg ggcatccggt ggcagaaggc gggtatcagt ttcaccagcc tttcggcagc
     9001 atcgcctgcc ttgcgccggg caatgtgttc cataaagcgt ccgcccagca ggaaaaacag
     9061 cagcatggcg atggattcga aatacatccc ctgccccgca ttggtcgcaa ggctgtaaac
     9121 gccggcgata aaggtcatga tgatggcgac ggcaatcggc gtatccatgc cggcgcggag
     9181 gtttttcagg tcgcgcaacg cgccttgata aaacgggacg gcgcaataga atacgacggg
     9241 cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt cgatgtcgcc
     9301 gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca gcccggcaac
     9361 ggcgaggcgg acgatgtatt gttttcgttc tttttggttg gcggcttcga ttttttgcgc
     9421 gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt cggaaaggcg
     9481 gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga ggtcgatgcg
     9541 gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg cggcgcaggt
     9601 gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca caaagtcgga
     9661 ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg gcggcagctc
     9721 ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg cgtcaataat
     9781 gctttgtgcg accgcctgac aaccggcgca gcaggtttcg cggtcttcgt tttcgtaacg
     9841 gacagtcagg tggaggtgtt cgggaacatc cagaccgcag tggaaacagg tttttttcat
     9901 ggcatttcgg tttcgtctgt gtttggtgcg gtggcacaat actcggcaat ggcttcgcgc
     9961 agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggtggcaat ttttcccgca
    10021 gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt tttgttgtac
    10081 accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc cacgtcttca
    10141 atctgctgtc cgctgttcgg ggcggcggca tcgacgacgt gcagcagcac atcggcttgc
    10201 gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt tgtgcggcaa atcactgacg
    10261 aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag ccgccgcgcc
    10321 gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt ggtcagccgg
    10381 ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt tttgattgtg
    10441 cccgattcgc ggggcttgcg gcgcagggcg cgctgttttt tgaggttgac aagctgtttt
    10501 ttcaaggcat tgatccgatg ggcgatcaat cggcggtcgg tttccagttt ggtttcgccg
    10561 gggcctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc gcgtatcaag
    10621 cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc ctgcgtgcgg
    10681 gcgcggcggg cgaaaatcgc cagaatcagc cctaccctgt ccaatacgcg gcatttcagt
    10741 tctttttcaa ggttgcgttc ctgcgtgggc gtgagttcgt ggttgaatac gaccaaatcg
    10801 atgccgtctg cggcaactgc ttctgacagc tccgccgcct tgcccgtgcc gacaaacagc
    10861 gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca cggaatcgcc gcccgccgct
    10921 ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac cgttcagacg ggtggcactg
    10981 ctgcccgtac catctttgtc caacattacg cccaccagca tgacgcgttc gggttgcgct
    11041 tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt caatcctgta aataaagggt
    11101 tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg cggctgcccg ccattccgtc
    11161 aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc tgccgggtcg
    11221 ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc agtggatgaa
    11281 atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa aataagggta
    11341 atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt tgcggacata
    11401 gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc ttgagcggga aaagcgcgaa
    11461 ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc tgacggggcg
    11521 gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc ctgccgcctc
    11581 ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg ggtctggacg
    11641 ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga taggctgtat agagcagctc
    11701 ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg cgttgcggta
    11761 atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct caaacctgcc
    11821 gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg ccgctcctgc
    11881 ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca ttaatccgtc
    11941 cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt cctcttccgt
    12001 gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc gttcggtcag
    12061 cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt cttcgggatt
    12121 gagcatgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg cgtgtgccag
    12181 cttgtgcaaa ccgatttggg ctcggatgcg gttgaggtag tgcagcgcat cgccgccgtc
    12241 cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt ggttttgggt
    12301 atggtagaaa acgcctgccg ccgaacccaa caggagcagc cgaataaaaa gggatttcat
    12361 aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac ttgccaatgc
    12421 ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg ccgaacccga
    12481 tgccgtctga accttcagac ggcatcgggt gtttatttgt ccacccggat gggggcaggt
    12541 tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg aatgcgtccc
    12601 tgcgatttgc ggtgggcatt ggaatcgcgc aaggaatagg cgcaaccgca atattcctgc
    12661 tggtagaagt tttcacgttt gctgatttca atcatgcgcg cgctgccgcc gcctttgcgc
    12721 cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcacggcg gccgcagtcg
    12781 ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa gacggggaac
    12841 ccgtgttcat gcgcgtattg cgccgccttt tcaaaacgca tatcgaaaca catggtgcag
    12901 cggatgccgc gttccggctc aaactccatg cctttggctt tggcaaacca ttctttgcgg
    12961 tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc cgaacttttc cgcaaagcgc
    13021 acgttttcct cttttcggag catatactct ttgtgcggat ggatattggg attgtaaaaa
    13081 taaatggtgt agtcgatgcc ggaggcaagc atcgcctcca tcacttcgcc gctgcacggg
    13141 gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag tatggggcgg
    13201 tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat atctgtcgaa
    13261 tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacaggggg tcagggcatc
    13321 agcccgagct gcctgaacga acggacggtt tgcgccgtta cgataaaatg gtcgagcagc
    13381 gacacatcga ccagcgacat tgcctgtgcc agccgccttg tgaacatgat gtcttcctgc
    13441 gaaggttcgg gcgagccgcc cggatggttg tgcgcaataa tcaggctgtc ggcatattcg
    13501 tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac cgtaccgcgc
    13561 gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc gacgctgact
    13621 tcgacttttt cctgccccaa atgaaagcgt aaataatcgg ccaccgtatc cggatcggac
    13681 aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc cctgaccacg
    13741 gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt tccgacctcc
    13801 gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc caaatcgacc
    13861 gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc atcactcaac
    13921 gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg ccattgcttg
    13981 atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag atgttcccac
    14041 acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc atctgtattt
    14101 caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccagaagc gacaggcgtg
    14161 ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag tctttttcag
    14221 agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct tcgggcgctt
    14281 cttgggaatt atcgccgtta ccggtaggaa tgctgttaga cagcggaaca aacaccatac
    14341 cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa aagccgccga
    14401 tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt aaggtttcat
    14461 aagtataaat cccttcttgg acaatcactt cgctgacttc caagccttct ttgactttcg
    14521 ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat ttgacgctca
    14581 tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt ttgtcggtaa
    14641 tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc gcttccgcca
    14701 acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca aaataagggt
    14761 taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact tggttgtacg
    14821 cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc ggcaaaacgg
    14881 tttggctgat gcctttgagg atgtcgggaa tgccggcaga caaatcgttg ttcaacaaaa
    14941 ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc aataaaggtt
    15001 ccaacaggat tttgtcgccc aatgcagttt caaattcggt cggttcggtt acttccggat
    15061 tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg agggcgtaaa
    15121 tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat acggatttcg
    15181 ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc gggataaagt
    15241 tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac gaaattttgt
    15301 aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgtacg ctccactgcg
    15361 tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca aacgcctgaa
    15421 gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg cctctgtgtg
    15481 ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt attctttcgt
    15541 atcgaatcat gctttagata taactgttta ctgaaatgga caatacgtct aattttttcg
    15601 ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt tgcgcggtat
    15661 aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag aacaacatca
    15721 tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc gaagaagaag
    15781 acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc agccctcagg
    15841 catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc gtcgtctcca
    15901 ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac ccgatttcca
    15961 agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc gcctattttc
    16021 cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa ggcgcgaccc
    16081 tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc gcattcggcg
    16141 cactggcgca cggcatcggc acttccgaag tcgagcacac gatggcgacc cagtgcatta
    16201 ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa gcgggcgtta
    16261 ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcgggc ggtacaggct
    16321 acgccgtcga gtttggcggc gaagccatcc gcagcctttc tatggaaggc cgcatgactt
    16381 tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc gaccaaacca
    16441 ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg gacaaagccg
    16501 tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa gaataccgtt
    16561 tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa atggttttag
    16621 acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa cgcagcggta
    16681 tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac gaaatccccg
    16741 tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg cgcgaagccg
    16801 ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta atcgtccccg
    16861 gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt ttcattgaag
    16921 ccggttttga atggcgcgaa ccgggctgtt caatgtgtct cgccatgaac gccgaccgcc
    16981 tcgcaccggg gcaacgctgc gcctccacct ccaaccgcaa ttttgaaggc cgtcaaggca
    17041 acggcggacg tacccacctc gtcagccctg ccatggcagc cgcagccgca gtgaccggtc
    17101 gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa gaatttgacg
    17161 cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa ccaaggagtt
    17221 taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc gcctgcgccg
    17281 gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca caggccgccg
    17341 ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac aaaaccaaag
    17401 aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc ggcggaaaaa
    17461 tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgctta agacggcatc
    17521 gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat cgtcgccccg
    17581 ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt aaaatccatc
    17641 aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga ccacggcgaa
    17701 ccgggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa ccagccgcgt
    17761 taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc ttcacgcgaa
    17821 cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc cagcttcgcc
    17881 gacatcttct ttaacaactg ctacaaaaac ggccttttac ctatcgtgtt gaccgaagaa
    17941 caagtcgatc ggcttttcaa agaagttgaa gccaacgaag gctatcagct ctccatcgac
    18001 cttgccgaac aaaccctgac cacaccaagc ggcgaaacat tcacattcga cattaccgaa
    18061 caccgcaaac actgcctctt aaacggcttg gatgaaatcg gtctgaccct gcaacacgcc
    18121 gacgaaattc acgccttcga agaaaaacgc cgccaaagcc agccttggct gtttaacggt
    18181 taagcctatg ccgtctgaag accgttcaga cggcattcct gataacatat aattgctgca
    18241 actaatgtaa acagaggggc tataaattgc tttcatcttt caagcgggct tagcaacgct
    18301 ctaaattaat gcaatacagc tctatttcaa ataaattttt agatttttaa tatgcaacag
    18361 attaaattta ttgacttatt ttccgggatg agcggtatca ggaaaggatt tgaacaagcc
    18421 tgtcggaaac agtcggttgc ttgcgaatgt gtttttacct cagaaatcaa accggcagct
    18481 ttggaagtat tgaagcaaaa ctaccctgat gaagtgccgt atggagatat aacgaaaatt
    18541 gaaacgggag atattcccga ttttgacatc ttgttggcag gcttcccttg ccaggctttt
    18601 tctttcgccg gaaaaagatt gggctttgaa gatacgcggg gaacgctttt ctttgatgtg
    18661 gcaaggattt taaaggcaaa aaaaccaaaa ggttttattt tggaaaatgt ggaaggattg
    18721 gtgacgcacg atagaaaaga tccgacacaa aaaataggac gcaccctgac cgttattttg
    18781 gaaaccttgg aaacattggg ctactatgtt tcttggaaag ttttaaatgc aaaagatttc
    18841 ggcattcccc aaaacaggaa gcgcatttat ctgacaggca gtttgaaatc caaacccgac
    18901 ttgtcttttg aaacaaccct aagtccgaaa ttaaaaaata ttttggaatc gggactgcct
    18961 accgaaagca gtcctttcat caaaaaattg ctaaagaaat ttcccccgtc cgaactgtac
    19021 ggaaaatcag taaaggacaa acggggaggg aaaaacaata tccacagttg ggatattgaa
    19081 ttaaaaggca cagtaaccga agaagagaag caattattaa atatgcttct aaaagaacgg
    19141 aggaagaaaa aatgggcttc agaaatcggc atcgattgga tggatgggat gcctttgaca
    19201 aaagcgcaaa tttcaacttt ctataaacac cccaatcttc aaaatatttt ggacagcctg
    19261 acagataaag gctatttggt tttggagcat ccgaaacaaa aaattggcgg gcgaagaatc
    19321 aaagatgaat ccctgcccaa aggctacaat attgtttcag gaaaaaaatc atttgaaatc
    19381 aataaaatat tagacccaaa tgatgttgcg ccaaccttag ttgcaatgga tatggagcac
    19441 cttttcgtcg ttgacaacgg cggtttgcgc acacttaccg gaaaagaagg tttacgctta
    19501 ttcggctatc cggacgatta ttcgtttgat attcctaaaa aagacaaata cgatttattg
    19561 ggcaataccg ttgccgttcc tgtgattaag gcggtatctg aaaggcttct gcatacttta
    19621 taaaccgata gggataaaat ggtaaaactt actgcacaac aaatatttga taagttgctg
    19681 aatgaggaaa aaatcttatc ggtcaatggt caaattagat ttttcttggg cgatgtggat
    19741 attatcgtca aacaaaaaga tgtcgtcggc aacatcattc aagaatggct cggcggatgg
    19801 ttgagaaaaa gggaaattga atttgatgtt tcaaccaata cccaaatgcc ccccgatttc
    19861 tttttaaata aaaaagaccg cagtagagaa ttgttggaag taaaggcgtt caaccgaaat
    19921 gccggcccgg gttttgatat tgcagatttt aaaatgtatt ctgatgaaat cattcataaa
    19981 ccctatatgc tggatgtaga ttatttaata ttcggttacg atatggacga caacggcaat
    20041 gtaaccatca aggatttatg gcttaaaaaa gtatggcaaa ttacgagaag catggatggg
    20101 tgggcaatta atcttcaagt caaaaaaggc gtggtgcata aaatccgccc gggtgtttgg
    20161 tacagcataa ataaaaagaa tatgcctatg tttgaatgct tggaagattt tgtttccgca
    20221 attgaagaaa ccgtttatca aaacccggca acacggcata acgcatcctt atggaaaaag
    20281 aaatttgagg aagcctataa aaaacactac aaccgatcga tttccatacc tcgttggcac
    20341 gaaattgcac acaaatacaa aaagaaataa cccttcaacc atcaacccat aaggaaactt
    20401 gaaatgacca aacatatcgc catcctccga ggtgacggca tcggtcccga aatcgtcgcc
    20461 gaaaccgtcc gcgtactcga caaactcatc gcccaaggct tggacatcag ctacgaatac
    20521 gccccattgg gtggtgaagc ctacgacgaa tacggccatc cttatcccga atttacgcaa
    20581 aacctctgcc gcaaagccga tgccgtcctg cttggtgcag tcggttctcc tcaatacgac
    20641 aacctcgacc gtccgttgcg ccctgagcgc ggcctgttgg caatccgtaa ggatttgaac
    20701 ctgtttgcca atttgcgtcc tgctgtttta tatcccgaat tagccaacgc ctccacgctg
    20761 aagcctgaaa tcgttgccgg tttggatatt ttgattgtgc gcgaactcac cggcgacatc
    20821 tatttcggcg aaccgcgcgg catccgcgtt ctggaaaacg gcgaacgcga aggctacaac
    20881 accatgaaat acagcgaaag cgaaatccgc cgtattgccc atgttgcctt ccaatccgcc
    20941 caaaaacgca gcaaaaaagt ctgttccgta ggcaaagcca acgttttgga aaccaccgaa
    21001 ctgtggcgcg aaatctttga agaaatcggc aaacaatatc ccgatgtcga actctcccat
    21061 atgtatgtgg acaacgccgc tatgcagctc gtgcgcgcgc ccaaacaatt tgacgtgatt
    21121 gccaccggca acatcttcgg cgatatttta tcggacgagg cctctatgct gaccggctcc
    21181 atcggtatgc tgccttctgc ttctttggac gaaaacggca aaggcctgta cgaaccgtct
    21241 cacggctccg ctcccgacat cgccggacaa aacaaagcca acccgctggc caccatcctc
    21301 tcgctcgcaa tgttgctgcg ttacagcctg aacgacgaag cgcgcgcgca acaagttgaa
    21361 aacgccgtcc aaaaagtgct gcaacaaggc ttgcgtacca gcgacattta cgaagaaggc
    21421 acaaaactcg tttcctgctc cgaaatgggc gatgcgatac tcgccgcctt gtaaaaaaac
    21481 aaagcgcgga tcacaccgcg ctttttcatc tgcccttttc cgcccgcacc gttcgtcaac
    21541 cgcctgcaaa cccaccgttt accggcagga aatgccataa atccccgcct atgttaggat
    21601 aaacaccttt atcggcccac acaaaggatt atcatcatga aaaaaatcat cttcgccgca
    21661 ctcgcagcgg cagccgttgg cactgcctcc gccgccacct acaaagtgga cgaatatcac
    21721 gccaacgccc gtttcgccat cgaccatttc aacaccagca ccaacgtcgg cggtttttac
    21781 ggtctgaccg gttccgtcga gttcgaccaa gcaaaacgcg acggtaaaat cgacatcacc
    21841 atccccgttg ccaacctgca aagcggttcg caacacttta ccgaccacct gaaatcagcc
    21901 gacatcttcg atgccgccca atatccggac atccgctttg tttccaccaa attcaacttc
    21961 aacggcaaaa aactggtttc cgttgacggc aacctgacca tgcacggcaa aaccgccccc
    22021 gtcaaactca aagccgaaaa attcaactgc taccaaagcc cgatggcgaa aaccgaagtt
    22081 tgcggcggcg acttcagcac caccatcgac cgcaccaaat ggggcgtgga ctacctcgtt
    22141 aacgttggta tgaccaaaag cgtccgcatc gacatccaaa tcgaggcagc caaacaataa
    22201 atcttgatgc gaatcagcac aaatgccgtc ttcatcaaac cgccccgcca acggtaagcc
    22261 gatatggcgg agcacaatcc gccgaaagat atttcagcgg caggcagggc ttgccggaaa
    22321 gtccggaaa
//
LOCUS       DAWKQJ010000015        22308 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000015 DAWKQJ010000000
VERSION     DAWKQJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.015"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(31..1557)
                     /locus_tag="WJL20_000446"
     CDS             complement(31..1557)
                     /locus_tag="WJL20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7682638.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQLEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(1669..3075)
                     /locus_tag="WJL20_000447"
     CDS             complement(1669..3075)
                     /locus_tag="WJL20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7682639.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            3359..4138
                     /locus_tag="WJL20_000448"
     CDS             3359..4138
                     /locus_tag="WJL20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ7682640.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4135..4836
                     /locus_tag="WJL20_000449"
     CDS             4135..4836
                     /locus_tag="WJL20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ7682641.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAIEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4833..6287
                     /locus_tag="WJL20_000450"
     CDS             4833..6287
                     /locus_tag="WJL20_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ7682642.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6470..6955)
                     /gene="purE"
                     /locus_tag="WJL20_000451"
     CDS             complement(6470..6955)
                     /gene="purE"
                     /locus_tag="WJL20_000451"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ7682643.1"
                     /translation="MTQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7004..7720)
                     /locus_tag="WJL20_000452"
     CDS             complement(7004..7720)
                     /locus_tag="WJL20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ7682644.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(7720..8388)
                     /locus_tag="WJL20_000453"
     CDS             complement(7720..8388)
                     /locus_tag="WJL20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ7682645.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8399..8584)
                     /locus_tag="WJL20_000454"
     CDS             complement(8399..8584)
                     /locus_tag="WJL20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ7682646.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8727..10703
                     /gene="mutL"
                     /locus_tag="WJL20_000455"
     CDS             8727..10703
                     /gene="mutL"
                     /locus_tag="WJL20_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ7682647.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIHVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAATHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDADLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10798..12927
                     /gene="dnaX"
                     /locus_tag="WJL20_000456"
     CDS             10798..12927
                     /gene="dnaX"
                     /locus_tag="WJL20_000456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ7682648.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHLLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    11987..12026
                     /gene="dnaX"
                     /locus_tag="WJL20_000456"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            13007..13342
                     /locus_tag="WJL20_000457"
     CDS             13007..13342
                     /locus_tag="WJL20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ7682649.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(13689..14735)
                     /gene="recA"
                     /locus_tag="WJL20_000458"
     CDS             complement(13689..14735)
                     /gene="recA"
                     /locus_tag="WJL20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ7682650.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGVDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            14989..15753
                     /locus_tag="WJL20_000459"
     CDS             14989..15753
                     /locus_tag="WJL20_000459"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ7682651.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            15773..17788
                     /gene="rep"
                     /locus_tag="WJL20_000460"
     CDS             15773..17788
                     /gene="rep"
                     /locus_tag="WJL20_000460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ7682652.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVTCQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPDDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQTFMDMFVSYLAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(17856..18914)
                     /gene="dinB"
                     /locus_tag="WJL20_000461"
     CDS             complement(17856..18914)
                     /gene="dinB"
                     /locus_tag="WJL20_000461"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ7682653.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            19227..19643
                     /locus_tag="WJL20_000462"
     CDS             19227..19643
                     /locus_tag="WJL20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682654.1"
                     /translation="MNKNILYIFSLLIAIVISFIFEKNVISKISFNYNKKEFLISDIT
                     NFNWDYVKLYIINSDFQKIVFYYKGKIVFEELIELDGEGNVLPQYLFDSDLENIEYYE
                     CDYKNGKMRLLKKEKSHFFDGYFYYYKPINCRPKLL"
     gene            19817..20596
                     /locus_tag="WJL20_000463"
     CDS             19817..20596
                     /locus_tag="WJL20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7682655.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(20807..22219)
                     /locus_tag="WJL20_000464"
     CDS             complement(20807..22219)
                     /locus_tag="WJL20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7682656.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLIAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGAINYIGTFERVYSEVSALLDSGKASFDWCNTEAVRFFP
                     ALGSLHAYKIKAELVRHYGADCYVSAKNLLKTFTTVRFEKGSDGMLDAKTAALKNGVA
                     DNPPTGLFVNKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT         5633 a         6196 c         5748 g         4731 t
ORIGIN      
        1 cgtactggtt tttgttaatc cgctatattt ctataataaa ctttctatgg gcagcaggga
       61 taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt
      121 tcgggtggcg ggatcgtgcc attgcaggcg gttgtgcctg tcgagggtaa cgcggtaggc
      181 gtattcgggg gtggtatcgg caagggtgcg ctccatctgt tctgcgattt tggggctttc
      241 gataacaacg cccatttcgg tgttgagacg cgcggaacgg gggtcgaggt tgaacgaacc
      301 gatgaagatg cgtttgccgt ccacaatgaa ggttttggcg tgcaggctgg ttacggagct
      361 gccggtcagg cctttgtctt ttgtggcagg gacggcatgg ttgggttgca gctcgtagag
      421 tttgatgccg gctttgagca gcggttttcg gtatttgaca tagccggaat ggacggcggc
      481 aacgtcggtc gcctgtagcg agttggtcag aacggtaacg tctatgccgt cctgcaccag
      541 ttttgccagt gcgtctgtgc cggattttgt ggggacgaaa tagggtgaaa ccagatagac
      601 gcttttttcg agctgtttga gcgcgtcttg cagccgcccg gcaatcggcg gtttgcggcg
      661 gtcgcggtcg agtccttttg cagggtcgtc gctgatcagg cgggtttgga cgctctgcca
      721 gtcgatgcgt cctgtctgta ttttttggta gaggggcgac tgttcgacgg tttcgcggta
      781 gcgcaggagc gcgtgtctgg acgtttcgtc gttgtatccg agtgcttgaa gacccttgcc
      841 gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg
      901 gtcgaagtcg tgcgatactt cgccgacgac gctgccggta gcgaggatat ccaggtcggc
      961 gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat
     1021 ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga ggcgggggaa
     1081 gtcagtcagg tagccgagtg cgcgccattt gcggaagacg aaggggttga acaggcgcac
     1141 ttcgatattg ggatggctgt cgagggcgag caggaggtcg tccaatccgc gcgtgttgtt
     1201 gtcgtccaac agcaggcgta cgcgcacacc gcgttctgcg gcaaggtaca cgaggttgaa
     1261 cagcagtcgg ccggaaatgt cgttgcgcca gatgtagtat tgcaaatcga ggctgtgttc
     1321 ggcagattcg ataagggcgg cgcgggcggc aaaggcttcg tgggggtcgt tcagcagata
     1381 gatatcggat agcccgttgg tatgaggggt gtgccggatt tgcaggatgt tgtccaggcg
     1441 gacgggtttg gaagtattga aatgacggct ttccgtccgt tcttccagtg ggggcaacca
     1501 tgaagaacat gaacagagaa ggaggcataa aagggaaatt aggctgcgtg ttttcatcag
     1561 ggatatggtt tcagacggca ttgcctgtgt tttggggttg gcgcgcatgg aagtgcggaa
     1621 tcataatcca aacgttgaaa cgggtaaaag ttttgcgtgc ggaccgcttt aggacggtgt
     1681 gttccgtgtc aggtttgcac cgtctgaaac gtgcagacgc ttgaaaacca acgacgaggc
     1741 aagggtgatg ccgccgatac tgagcagggt catacggaag gcggaatgca gacctgaaga
     1801 agccggtatc agaaatgtcc agtttttaag gattaatgcg ccgacaacaa tgcccatgct
     1861 gatggcaagc tgttggttga ccgccatcag gctgttgccg ctgccggtct gttgcgggcg
     1921 caaatcggcg agggtcagtg tattcatggc agaaaactgc agggagttgc acgcgccgat
     1981 cgccagcgag aggaaaatcc aaatccacag cgacgagttt ccgtcaggca gggcgagcag
     2041 catgatgaag gcggcaagca gcttggtgtt ccaaagcagt accgtgcggt agccgaaacg
     2101 tttcatgagc ggtgcaatca gcggtttgac cagcagcgaa gacagggcga cgggggcgac
     2161 cagccaaccc gacaggcttg cgccgaagcc gaaagcgact tgaaacatca ggggcatcag
     2221 aaaaggaatc gagctgatgc cgagacggct gaacagattg cccgccagtc ccagacggaa
     2281 agtgcgtatc agaaacaggt cggcggaata aatcggtttg gacgcggttt tcatatgtcg
     2341 gaaataacgg cgtgcaaaca gcagtccgcc gcacagcggc aacagtgcaa aatacggagg
     2401 cagcgcgtgc gacaggcttt ctgccgaaag taacaagagg cacgcggcgg cagaaaaaat
     2461 cagatagcct ttgaaatcca gagcggtatc gttgcctttg acatcgggca tgatgttgcg
     2521 tcccaatatg aaacccagca gaccgatggg caggttgagc aggaaaatcc agtgccacga
     2581 agcgtattcg accaaataac cgcccgccaa aggccctaaa accggcccga ttaatgcagg
     2641 catgaccgca taattgatag cattgagcag cttggacttg tcgtacatac gcaagatggt
     2701 cagacgcggt atcggaacca gcatcgaacc gccgatgccc tgaacgacac gggaaagcgt
     2761 caattcaaac agcgaacccg atgcggcgca caatgccgat ccgagcataa aaacggcaat
     2821 cgaaccgaaa aagacttttt tcgttccgaa cctgtccgcc aaataaccgc tcaaaggaat
     2881 cagcagggca accgtcagcg tataggcaac gactgccagt tgcatatcca gaggcgactc
     2941 attcatgtcg gcggcaattt caggcagtgc ggtatttaaa atggtcgcat ccaacatctg
     3001 cataaaaatg gcaattgcca gcagaagcgg cagccaaggg gatggtgcgc gggcggatag
     3061 ggtgtttttt tccatagggc gattgtaccc catccttgtg ccgttattgt tttcagatgc
     3121 tgtctgaatg ccgtcagagt cggcgtcctg aatgttcaca agcaaacgaa tcagcatttt
     3181 attgtaatga taattattat cgaaaattat cagagtaagg tacagtaagc gttatggggc
     3241 agtttgtaaa aaaaaccgga ttatttttta aaattagact tgacccgcaa cagtcaatta
     3301 cttaaagtaa acgcttacct ttctacagag aaaaacgggt ttcccgttat caaaaaacat
     3361 gagcgcaacc attcccccaa aaatcatccg atacgacagc aatccgaccg atgtctattt
     3421 tttcggcact tgcgtccttg atctttttat gcccgaagca ggcatggatg ccattgccct
     3481 aatcgagcag cagggcatac gcgtccattt cccgatggcg caaagctgct gcggccagcc
     3541 tgcctattca tccggccatc cgaccgaagc cttcgatgtc gccaaagcgc aacttgacct
     3601 tttccccgaa aactggccga ttgtcgtacc gtccggttcg tgcggcggta tgatgaaaca
     3661 ccattggccg acgctgttta aaggcagcga gtacgaggaa agggctgtgg attgcgccgg
     3721 ccgcatcatc gagtttaccc atttcctgct tgccatcggt ttcaaacccg aagacaaggg
     3781 cgaaccgctc aaagtcgccg ttcacacttc ctgcgccgcc cgccgcgaaa tgaatgtcca
     3841 tctttcaggc tggcaactga ttgacggtat ggaaaacgtc gaacgcatcg tccacgacca
     3901 cgaaagcgaa tgttgcggct tcggcggcac attctccgtc aaacaagccg atatttccgg
     3961 cgcgatggta accgacaaag tcgccgcgct gaaagaaacc ggcgcaaccg aaatcatcag
     4021 cgcggactgc ggctgtatga tgaacatcgg cggcaaaatc gccaaggacg agccggatat
     4081 gccgcgtccg aaacatatcg catccttctt gttggaacgc accggaggca aagcatgagc
     4141 gcgcgtgaaa atattttggc aaaactgaaa aaagccgatg ccttgccgat ggaagagcct
     4201 gcggttttcg attattaccg cgaaaagggc gtttcttggg acagcgaagc cgagcgtctg
     4261 aaacattggg ctgccgcaat gcgcgcggtc aaaaccgaaa tttattgggt gacgaaaagc
     4321 aattggatgc aggttttccg cgaagcggca gaaggcaagg gtttgaaaaa catcctgctg
     4381 cccttggcga ccgaacacgg acaaattgcc cgtgccgcat tggcgggcag caatatcgaa
     4441 ccgattgtct tcgagcgcga aatcgatact tggaaaaccg agtttttcac gaacatcaat
     4501 gcgggcttca gcggcgcgca atgcggcatc gcccgcaccg gcacgctgat gctgttttcc
     4561 agccccgaag aaccgcgtac tttgagtctc gttccgcccg tgcatttctg cctgttcgat
     4621 acgtccaaga tgtacaacga gtttcataat gccatcgaag gcgaaaaact ggtggaaaac
     4681 ggtatgccga ccaatgtatt cctgatttcc ggcccgtcca aaaccgcaga catccaactg
     4741 acgcttgctt acggcgcgca cggcccgcgc gatttggtca tcctcgccat cctgcccgac
     4801 cacatttccc ctgccgattt ggaggaaaac gcatgactac gcaaaccatc aaatttcaca
     4861 tgaagccgga aactttcaag caaaacgccg caatttccct tcaagacaag cctttgcgca
     4921 aaagcctgcg taccgcgatg gatatgctga tgaccaaacg caaagccgtt ttgaccgacg
     4981 aagaagagct gcaaagcttg cgcgatttgt gcgaacacat ccgccagcgt tccctgtcca
     5041 aattgcccgc gctgttggag cagttggaag aaaacctgac caagttgggc gtaaaagtac
     5101 actgggcgga aacaccggcc gaagcctgcg aaatcatcca cgacatcatc acagccaaaa
     5161 acggcaagct gatggtcaaa ggcaaatcga tggtcagcga ggaaatcgag ctgaaccatt
     5221 atcttgaagc aaaaggcatt aaagcggtag aaagcgactt gggcgagttc atcgtccaaa
     5281 tggcaggcga aaaaccgacc catatcgtga tgcctgcaat ccataaaacc aaagaacagg
     5341 tgagcgaact gttccaccaa aacctcggca caccgcttac agacgatgtg gaccaactga
     5401 ccggcttcgc ccgcaaagcc ctgcgcgata tttacagcac tgccgatgtc ggcttgagcg
     5461 gcgtgaactt cgccgttgcc gaaacaggta cgctgtgtct ggtggaaaac gaaggcaacg
     5521 gtcgcttgag taccaccgta ccgcccgtgc atatcgctat taccggcatt gaaaaagtgg
     5581 tggcgaaatt gtccgacatc ccacccttgt acagtctgct gccgcgctcc gccatcggtc
     5641 aaaacatcac aacctatttc aatatgatta ccggcccgcg ccgcagtgaa gaattagacg
     5701 gtccgcaaga aatgcacttg gttctgctcg acaacggccg cagccaggct tatgccgaag
     5761 accaaatgcg ccgcaccctg caatgtatcc gttgcggcgc gtgtatgaac cattgcccgg
     5821 tttatacccg catcggcggc gcggcatacg gcacaaccta tcccggcccg attggcgaga
     5881 ttatttcccc tcacctgttg ggtctggatg ccacccgcga tctgccaacc gcctgtacca
     5941 tgtgcggtgc gtgtgtggaa gtctgtccgg tacgcatccc gattaccgaa caaatgcagc
     6001 gtttgcgcgt tgaagcgcaa cgttcgccga ccgaaaccgt gccgcacccc atccgagggc
     6061 aaggcgcatc gcataccttc ggcgaacaaa tggcgtggcg cacattcaac ggtattttca
     6121 gcggcagcaa aacctaccgc gccttcggtt gggcagccac caagttccgc aacctgaccc
     6181 cgcgcaaaca gttgggttgg acgcaaaacc gcgtgccgat gaaaccggcg aagaaaaccc
     6241 tgcacgaact gatggcagaa aaaatgcgcc aaaaagaaca ggcataaaca gttgtttgca
     6301 aaaatgccgt ctgaaacccg aaacagggct tcagacggca tttgtatagt ggattaacaa
     6361 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggtaac
     6421 gccgtactgg tttaaattta agccactata tattcgcgga cggcgggttt taaatctgtt
     6481 ccaattccat actcaaaaca gcctgttcct gtttggctcg gaagtctgcc agtttttgcg
     6541 ccagttcggg gttttcgttg gccagcatgg aaacggcgaa caatgcggca tttgccgcgc
     6601 ctgcctcgcc gatggcgaat gtggcgacgg gtacgccttt gggcatttgt acaatcgata
     6661 aaagcgaatc ttcgccgcgc aggtatttgc tggggacggg tacgcccaaa acggggacgg
     6721 tggtcttggc ggcaaccatg ccgggtaaat gcgccgcgcc gcccgcaccc gcgatgatgg
     6781 ctttgatgcc gcgcgcccgt gcggtttcgg cgtattggaa cattaaatcc ggagtgcggt
     6841 gtgcggaaac gacgcgcgcc tcatattcta cgccgaactc ttcaagaaac tgcgctgcct
     6901 gccgcataac gggccaatcg ctgttgctgc ccatgatgat gccgatttgt gtcataaatc
     6961 tctccttggt gcggatgggg taaaaagcgg aaaaatggaa aaactatcgt ttgcgtacgg
     7021 ctgcggcggc gcgttttgcc gccgggctgc tcgggtaagc ctgtatcagg ctgcgccaag
     7081 ttgcccttgc aatgtctttc tgctgcaacc tgtattggca ttcgccgatt ttgaacatgg
     7141 cttcgggcgc ggttgggctg tctttgaaac ggttggcgta acgccctccg atttcgatga
     7201 cggattcgca gttgcccata cgcgccctgc tttgcagcaa caggtacata ctgcgttgcg
     7261 cgatgctgcc gccgtcgcct ccgtccgcgc ctttcaacag ggaggcagcg gcagaaaacc
     7321 tgccgctttt atagtgtttg agtgcctgat tgtagaggtt ttgtgcggtt tcgacggtat
     7381 gtgcggatgc gctgccgcct tcggtattga ggtaatgctc tttcaacttg cggtcgtcga
     7441 gtttttggac gtatgccctg ctggaagggt gtgttttcgc atgctccagt gctttgactt
     7501 tgccgtttaa ggtttccact tcgttcgaca ggcggacgag tgtgccttcc agataatcca
     7561 aacggtcttg cagagtggga acgggatagg gaatagcgtc cgaactacct gcctctgcct
     7621 gcggttcgag ccgcatatcc tgaatattgc gggaaacagg ggaagaacag gcggcggata
     7681 cggacagcca aatgataaaa agcggtaatt tggtcttcat tattttttca gaagcagggt
     7741 caaaccgtcg ccgacgggca gggtgatggg gacgatgcgc gggtcgttcg gcaggttttg
     7801 attgaaatct ttgaggatgc cgacactggg cggcgcgtcg gaagccgctt cgcgcatcac
     7861 ccttccgttc aacaaaatat tgtcaatggc aatgatgccg ccttgccgga cgagtttgag
     7921 gcaacgctcg aaatattgcg gcgtgggcgg tttgtctgcg tctatcagtg ccaaatcgta
     7981 gcttccggct tcaccctgta caatcaaatc atccaatgtc agcaatgcgg gttgcaggtg
     8041 caggctgatt ttatgtgcca cacctgcctc gttccaaacc tgacgcgccg tatcggtaaa
     8101 ggttacattg atgtcgcagg cggtaatccg cccgtgttct ggcaacgcca atgcaagcgc
     8161 ggtgctgctg tatccggtaa acacgccgac ttccagatat ttttccgcgc gaatcagctt
     8221 tgccagccaa accaaaaccg ccgcctgttc gcgcgcaatc gccattttgc ccatacggtg
     8281 atgctcggtc ttctcacgca gccgcgtcaa aacgggatgt tcgggttcgc cgatggcgtt
     8341 caaatagttt tgcaggtcgg gggcgacatt ggacagatgg gtcgtcattt cggcgggttc
     8401 agtcttggta ataggtataa ggttttttcg ctacttttgc cgcctcgaag ttttcctgtt
     8461 cttcgggatt gagttcgaca tcccacaaaa gcccccggtt ttccaaacgc tgctgttcca
     8521 actcaggttt ttcttcaatc agacggttga ggaattgtgt ggcttcggat tggtagtgat
     8581 acatctttgc gctccaattt tacgggatac ggcgtgatta tactggtatt ttccaaacgg
     8641 gataaacggc ttttatcaag aatacaggca gaaaagtaag gggttttatt atagaataaa
     8701 gcgtttttgc aacggaagcc taccttatgc cacgcattgc cgccctgccc gaccatctcg
     8761 tcaaccaaat cgccgccggc gaagtagtcg aacgccctgc caacgccttg aaagaaatcg
     8821 ttgaaaacag tatcgatgca ggcgcaacgg cgattgatgt cgagctggac ggcggcggca
     8881 tccgcctgat tcacgtcagc gacaacggcg gcggtatcca tccggacgac atcgaacttg
     8941 cgctccaccg ccacgccacc agcaaaatca aaaccttaaa cgatttggaa cacgttgcca
     9001 gcatgggctt tcgcggcgaa ggcttggcaa gcatcgcctc cgtcagccgc ctgaccctga
     9061 ccagccgcca agaagacagt tcgcacgcaa cccaagtcaa agccgaagac ggcaaactca
     9121 gcagccccac cgccgccacc caccccgtcg gcaccaccat cgaagccgcc gaactcttct
     9181 tcaacactcc cgcgcggcgc aagttcctca aatccgaaaa caccgaatac gcccactgcg
     9241 ccaccatgct cgaacgcctc gcgctggcgc atccgcacat tgccttctcg ctcaaacgcg
     9301 acggcaaaca agtgttcaaa ctccctgcac aaagcctgca tgaacgtatc gccgccattg
     9361 tcggcgatga ttttcagacg gcatcattgg aaatcgacag cggcagcggc gcgctgcggc
     9421 tctatggtgc gattgccaaa ccgactttcg ccaaaggtaa aaccgacaaa caatactgct
     9481 tcgtcaacca tcgcttcgtg cgcgacaaag tgatgctcca cgccgtcaaa caggcatacc
     9541 gcgacgtatt gcacaacgcc ctcacccctg ctttcgtcct ctttctcgac ctgccgcccg
     9601 aagccgtgga tgtcaacgtc cacccgacca aaaccgaaat ccgcttccgc gacagccggc
     9661 aggtgcacca actcgtgttc cacacgctca ataaagccct tgccgacaca cgcgccgact
     9721 tgaccgaaag cgtcagcaac gcaggcgaag tgctgcatga cattaccggc gttacgcctg
     9781 caccaatgcc gtctgaaaac gacagcgaaa atctgtttaa tcgcgcatcc gactacccga
     9841 caggcaacaa acccgatacg cgcaatgcct ttggtgcatc aggcaaaacc gcgcctatgc
     9901 cctatcagtc cgcatatgcg ccgcaacaac gcagcctgtc cctgcgcgaa agccgcgcgg
     9961 caatgaacac ctatgccgaa ctctacaaaa aaaccgacga tgccgacctt gagttaagcc
    10021 gattcgagca ggcacgtttc ggcaatatgc cgtctgaaac gcctgctccc caaacagata
    10081 cgccgctttc agacggcatc ccgtccccat ccgaactgcc gccgctcggt ttcgccattg
    10141 cccaattgct aggcatctac atccttgccc aagccgaaga cagcctgctg ctcatcgata
    10201 tgcacgccgc tgccgaacgc gtcaactacg agaaaatgaa acgccaacgg caggaaaacg
    10261 gcaagctgca aagccaacgc ctgcttattc ccgtaacctt tgccgcgtcc cacgaagaat
    10321 gcgccgccct cgccgattat gccgaaacgc tggcaggctt cggcttggaa ttgtccgata
    10381 tgggcggcaa caccctcgcc gtccgcgccg ttcccgccat gctcggcaaa gccgatgtcg
    10441 tctccctcgc caaagacgta ttaaacgaac tcgcccaagt cggcagcagc caaaccatcg
    10501 aggaacacga aaaccgcatc ctcgccacga tgtcctgcca cggctcgatc cgcgccggcc
    10561 gccggcttac cctgcccgaa atgaatgcgc tcctgcgcga tatggaaaac acgccgcgca
    10621 gcaaccagtg caaccacggc cgtccgactt gggtcaaact gactttgaaa gaattggacg
    10681 cactgttctt gcgcggacaa taagccgaaa gtgctagaat acgccgcccg agaccgccgt
    10741 tcagacggca ttccgacgca ccgacagaaa catcacgacc gaaaccaaga gaaaaatatg
    10801 gcctatcaag ttctcgcccg aaaatggcgg cccaaaacct tttccgacct agtcggtcag
    10861 gaacacgtcg tcaaagccct gcaaaatgcc ttggacgaag gcaggctgca ccacgcctac
    10921 ctgctgaccg gtacgcgcgg cgtgggcaaa accaccatcg cccgcatcct tgccaaaagc
    10981 ctcaactgcg aaaacgcgca acacggcgaa ccttgcggcg tatgcgaaag ctgtacgcag
    11041 atcgatgccg gacgctatgt cgacctgctc gaaatcgacg ccgcctccaa cacaggcatc
    11101 gacaacatcc gcgaagtctt ggaaaacgcc caatacgcgc cgaccgccgg caaatacaaa
    11161 gtctatatca tcgacgaagt gcatatgctt tccaaaagcg cgttcaacgc tatgctcaaa
    11221 acgctggaag agccgcccga acacgtcaaa ttcatccttg ccacgaccga tccgcacaaa
    11281 gtccccgtta ccgtattaag ccgctgcctg caattcgtct tacgcaatat gaccgcgcag
    11341 caggttgccg accaccttgc ccacgtcctc gacagcgaaa aaatcgccta cgcccccccc
    11401 gccctgcaac ttttgggacg cgccgccgcc ggatcgatgc gcgatgcctt gagcctgctc
    11461 gaccaagcca tcgccatggg ttcgggcagc gttgccgaac aagacgtccg ccaaatgatc
    11521 ggtgcggtcg ataaacaata cctgtacgaa ctgctgacag gcatcgtcaa ccaagacggc
    11581 gaagccctgc tggcaaaagc acaggaaatg gctgcgtgtg ccgtcggctt tgacaacgcc
    11641 ttgggcgaac ttgccatact gctgcaacac ctcgccctga tacaggcagt gccgaatgcc
    11701 ttggcgcacg acgaccccga ttccgatatt ttgcacctcc tcgcccaaac cataagcggc
    11761 gaacaaatcc agctttacta ccaaatcgcc gtccacggca aacgcgacct gagcctcgcc
    11821 cccgacgaat acgccggctt tatgatgacc ctgctgcgta tgctggcgtt cgcacccttg
    11881 gcggcaacat cgtgtgatgc aaacgccgtg attgaaaata ccgaacttca atccccatcg
    11941 gcacaaaccg ccgaaaagga aaccgccgca aaaaagcccc aaccgcgccc tgaaacggaa
    12001 accgcccaaa catccgttca gacggcatcc gcagcggcaa tgccgtctga aagcaaaact
    12061 gccgaacccg tttccaatca agaaaacaac gatgttccgc cttgggaaga cgcgccggac
    12121 gaagcccaaa ccgcagccgg cacggcgcaa acgtcggcaa aaagcattca gacggcatcc
    12181 gaagccgaaa cgccgcccga aaaccaagtt tccaagaaca aggcagccga caacgaaacc
    12241 aatgccccct tgtccgaaac gccgtctgaa aaccccattc aggcaacacc gaataatgaa
    12301 gccgttgaaa cggaaacatt tgcacacgaa gcccctgcag aacctttcta cggttacggc
    12361 tttccggata atgactgccc cgtagaagac aacgcagaaa tcccgccgcc cgattgggaa
    12421 cacgccgccc ctgccgatac cgcaggcgga ggcgcggacg aagaagcaga agcgggcggc
    12481 atcggcggaa acaacacgcc gtccgccccg ccgcccgaat tttccaccga aaactgggca
    12541 gccatcgtcc ggcatttcgc ccgcaaactc ggcgcggcgc aaatgccggc gcaacactcc
    12601 gcgtggacgg aataccatcc cgacaccggc ctgatggttt tggcgatgac cgccgaagcc
    12661 cgcgccaccg ccgacaaaaa acgtctcgac aaaatccgcg acacccttgc ccaagcctac
    12721 ggactgcaac tcaccctgca aacccaagac tggcgcgacg aagccggcag ggaaaccccc
    12781 gcgatgcagg acaagcgcgt ccaagccgaa gacaggcaaa aagcacaagc attgctcgaa
    12841 gccgaccccg ccgcacaaaa aatcctccaa gcattcggcg cgcaatggca gcccgaatca
    12901 ctggaattgg cagcaaaccg gccataaaca gatataatgc cgcccgaacc cttcggacgg
    12961 cattgccgtt tccctcattc aatcaaaaca gacaggagta ttcagtatgt tcggaaaagc
    13021 cggattaggc ggcctgatga aacaggcgca gcaaatgcag gaaaatatga aaaaagcgca
    13081 agccaaactt gccgaaaccg aaatcgaagg cgaagcaggc aacggcttgg tcaaaatcac
    13141 aatgacctgc gcgcacgaag tacgcaaaat cgacatcagc cccgatttga ttcaagaagc
    13201 cgccgacgac aaagaaatgc tcgaagacct catcctcgcc gccctcaaat ccgcccgtga
    13261 caaagccgaa gaaaccgcaa acaaaacaat gggcgcattc acgcaaggtc taccccccgg
    13321 agtgggcgac ttcttccgct gatacccaac cgtcattccc acgcaggcgg gaatctagaa
    13381 cgtagaatct aagaaaccgt tttactcgat aaatttccgt gccgacgggt ctagattccc
    13441 gcgttcgcgg gaatgacggc atattttttg catttgatat aaagggtcgt ttgaattttg
    13501 ttcagcaagt gcaaagtgtt gcacataaaa gggcgcagga tagaggcaaa gcgggcgtag
    13561 gtcgggctgt agcaactgta tttttcaccc cgtcgggcaa aaataccaaa actcaaatca
    13621 agccgttcgg aggcggctca aaaaacggta cttcgcagca gaagtaccgt ttatcgggat
    13681 ttcaggtttt attcttcggg gcgttcgccg tcggtttcgt cctgcgtccc ttcggtgatg
    13741 tgcatttcta cgccgttgag ggcgcggatt tttgcgtcga tttcatcagc aatttcggga
    13801 ttttccttca gccagacgcg gacgttgtct ttgccctgac cgattttcgc gccgttgtag
    13861 ctgtaccacg cgccggattt gttgatgatg tcgtttttca cgccgatgtc gatcaattcg
    13921 ccttcccaac tgatgccttc tccgtagagg atgtcaaact ctgcctgacg gaacgggggg
    13981 gcgactttgt ttttgatgac tttgacgcgg gtttcgttgc ccaatacttc ttcacctttt
    14041 ttgatggatc cggtgcggcg gatgtcgagg cggacggaag aatagaattt cagcgcgttg
    14101 ccgccggtcg tggtctcggg gctgccgaac attacgccga tcttcatccg gatttggttg
    14161 atgaacacaa ccagcgtgtt ggtttttttg atgtgtccgg tcagtttgcg caaagcctga
    14221 ctcatcaggc gcgcctgcag tccgacatgg ctgtccccca tatcgccttc gatttcggct
    14281 ttggggacga gtgcggctac ggaatcgacg actaccatat ctacgccgcc ggagcgcacg
    14341 agcgtatcgc agatttccaa agcctgttcg ccggtatcgg gctgggagag gtaaagctct
    14401 tcgactttta cgccgagttt gcgggcgtaa acgggatcga aggcgtgttc ggcatcgaca
    14461 aaggcgcaca cgccgccgtt tttctggcat tgggcgacgg cttcgaggca gagggtggtt
    14521 ttgccggagg attcggggcc gaagatttcg acgatgcgcc cgcgcggcag accgccgact
    14581 ccgagggcga ggtctaatcc gagcgatccg gtggaaatga cttcgaggtt ttcttcctgc
    14641 tggctgccgt ccattttcat gatggcgcct ttgccgaaac ttttttcgat ttgcgccagt
    14701 gcggcggcaa gggctttgct tttgtcgtct gacattgggg ttactccgga acaaatgcgg
    14761 tatgtgggat gcggcgcaac acgggctgcg gcgcgggatt tgtatcgttt tcccgatgtg
    14821 cgggctatcg gtaatgctgc ttcacggggt tgccattatc gcatatttcc ttgcttgccg
    14881 atatgcggca ggacgcggcg gcttgtgccg gaatggaatc tggatgccgt ctaaaaggcg
    14941 gccggctttg ttataatggc ggctgttttt tctgtgtgtg cctgttttat gtgttcctgc
    15001 cttgttgtca aaaataccgt tatcggaagc ggacgcacca aaatcgccgt gccgcttgtc
    15061 gcccgcgatg ccgccgtact ttccgccgta cttgaccaaa tcaaaaacct gcccttcgat
    15121 atcgtcgagt tccgcgccga ctttttggaa tgcgcgggca gcatcggcga agtattgcgc
    15181 cacacgcaga ccgtccgcga cgcgctgccc gacaaaccgc tgctgtttac gttcagacgg
    15241 catggcgaag gcggctcatt cccgtgttcg gacgattatt attttgaact gctcgacgcg
    15301 ctgatcgaaa gccgcctgcc cgacattatc gatattgagc tgttttccgg cgaaaccgcc
    15361 gtccggtgcg ccgtggcaaa tgctcaaaaa aacggcatcg ccgccctgct ctgcaatcat
    15421 gagtttcacc gcacgccgcc gcaagaagaa atcgtatgcc gtctgaaaca gatggaggac
    15481 tgcggcgcgg acatctgcaa aattgcggtg atgccgcaaa gcgcggaaga tgtgctgact
    15541 ttgctttccg ccacgctcaa agcgaaagag cttgccgcca aaccgattgt tacgatgtcg
    15601 atggggcaga cgggggcggt cagccggctt gccggacagg tgttcggctc aagcatcacg
    15661 ttcggttcgg gaacgcaaaa ctccgcgccg gggcaaatcg gcgtatccgc cctccgtgcg
    15721 acactcgact gcctcgaaaa cggcgcagac tgatttcaga cagcatcaaa acatgatgaa
    15781 actcaatccc caacagctcg aagccgtccg ctacctcggc ggcccactgc tcgtccttgc
    15841 cggtgcaggc agcggcaaaa ccggcgtgat tactcaaaaa attaagcatt tgattgtcaa
    15901 tgtcggctac ctgccgcata ccgttgccgc aattaccttt accaacaaag ccgccgcgga
    15961 aatgcaggag cgcgtcgcca aaatgctgcc caagtcgcaa acgcgcgggc tgacaatttg
    16021 cacgttccat tctttgggca tgaagatttt gcgcgaagag gcgaaccata tcggttacaa
    16081 aaaaaacttc tccattctcg attctaccga cagcgcgaaa atcatcggcg aactcttagg
    16141 cggtacgggc aaagaagccg tattcaaggc gcagcaccag atttccttgt ggaaaaacga
    16201 tttaaaaacg cctgaagatg tcgttcagac ggcatcgaac atttgggaac aacaaaccgc
    16261 acgcgtgtat gcgagctatc aggaaacctt acaaagctat caggcagtgg acttcgacga
    16321 cttgatccgc ctgcctgccg tgctgttgca gcaaaacagc gaagtgcgca acaaatggca
    16381 gcggcggctg cgttatctgc tggttgacga atgccaagat acgaatacct gccaatttac
    16441 gttgatgaaa cttctgaccg gcgcggaagg tatgtttacc gccgtcggcg acgacgacca
    16501 gtccatctac gcatggcgcg gcgcgaacat ggaaaacctg cgcaaaatgc aggaaaacta
    16561 tccgcagatg aaggtcatca aactggagca aaactaccgc tccaccgcgc ggattctcaa
    16621 aatcgccaac aaagtcatcg agaacaaccc caagctgttc accaaaaaac tttggtcgaa
    16681 attgggcgaa ggcgagcccg tcaaagtcgt tacctgccaa aacgagcagc acgaagccga
    16741 ctgggtcgtc agccaaatcg tcaaacaaaa actcatcggc ggcgacaaaa cccaatatgc
    16801 cgatttcgcc gtgttatacc gcggaaagca tcaggcgcgg attttcgaag aagcattgcg
    16861 cggcgcgcgc atcccctacc ggctctcggg cggacaaagc tttttcgaca aagccgaaat
    16921 caaagacgtg ttgtcttatg tgcggctgct tgccaacccc gacgacgatc ccgcctttct
    16981 gcgcgccgta accacgccca aacgcggcat cggcgatgta acgctgggca aactcaacac
    17041 ttacgcgcac gaacacgaat gcagcctgta tgaagccgcg caaaacgaag aagcccttgc
    17101 cacgctgaac aataccaacc gccaacacct gcaaaccttt atggatatgt tcgtcagcta
    17161 cctcgccaaa gccgaaacca gcgaagcggg cgagttcatc aacagcctgc tcgaagaaat
    17221 cgactatgaa aaccatttga tgcaaaacga agaaggcaaa gccggcgaaa tcaaatggcg
    17281 caacgtcggc gatttggtat catggtttgc gcgaaaaggc ggggaagacg gcaaaaacat
    17341 catcgaactc gcccaaaccg tcgccttgat gacgcttttg gaaggaaaag acgaagaaga
    17401 aaccgatgcc gtctcgctat ccacgctaca cgccgccaaa ggtttggagt atccgtatgt
    17461 tttccttgtc ggttgcgaag aaggcgtttt gccgcacaac gacagtatcg aagagggcaa
    17521 cgtcgaagaa gaacgccgcc tgatgtacgt cggcatcacc cgcgccaaac gccaactcac
    17581 actgacccac tgcgtcaaac gcaaaaaaca aggcacatgg cagttccccg aacccagccg
    17641 attcatagac gaaatgccgc aggaagattt gaaaatcctg ggtcgcaaag gcggcgagcc
    17701 gattgtcagc aaagaagaag gcagacgcaa ccttgccgat ataatcggaa ggctcgacaa
    17761 cctaaaaaaa agcggcgcgg cggattaaac cggagccgca atgccgtctg aaggcttcag
    17821 acggcatatt ttttggacgg cgcgcgtaaa gcggtttacg cccacaaatc ctgctgctgg
    17881 tttttcggca caagatgccc cacgccgata ccgataaggc ggaacgcgtc ttccgtctgc
    17941 ggcgggacgc gcgccatcaa catttgcgca gcctgcagca gagctgcgca gtcgggcaat
    18001 acggaggaat aagtcagtgt gcgcgtgatg atgcggaaat cgtaggtctt cagcttgagc
    18061 gttacgcttt gggcttcgac gtttttgcgc gtgatttgcc gccacaggtc ttcagcaaga
    18121 tggggcaggt gtccggcagc ctgctcgagc ggcaggtctt caggcagggt gatttctgtg
    18181 gagatttgga ggcgttcgcg ttcggctttg acggggcgtt catccgtacc gcgcaccaaa
    18241 tcatagaggc ggtatccgta gcgtccgaaa tggtttaaga gctcgccgcg ctcgaaacgg
    18301 cgcaagtcgc ccgccgtccg catacccagc gactgcattt ttttcagcgt aaccttgccc
    18361 acgccgggga ttttgcccaa aggcagggtt tccaaaaatg ccatgacttt gtgcggcggc
    18421 aacacaaact gcccgttcgg cttgcgccag tccgacgcga ttttcgccag aaatttgttc
    18481 ggcgcgatgc ctgcggatgc agtcaaacct gtttccgcaa aaatggcggc acggatttct
    18541 ttggcaactt cactggcgta agggatgttt ttgaaattac gggtaacgtc aagataggct
    18601 tcgtccagcg acaagggttc gattaaatcg gtataacgcc tgaatacggc gtgaatctgc
    18661 gcggaaacct gacggtacaa atcgaaatgc ggcggcacat acaccgcttg cggacacagc
    18721 cttttcgccg ttgccaccga catcgcggaa tgcagcccga actgccgtgc ctcatacgat
    18781 gcggcgcaaa tcaccgaacg cgcgccctcc cacgcgacga ccaccggccg ccctttcaaa
    18841 tgcggctgtt cgcgcagctc taccgatgcg tagaacgcgt ccatgtcgat gtggatgatt
    18901 ttgcgtgaag acatcggctc tgctgaggat aaaagggata ttctactgcc ggcatcgggc
    18961 aaattccaaa tatactaaca ggttaaatac aaaaaagttg gctggattaa ttttggaagc
    19021 ttataaaaat aatggttttt atcaagcaga aagaaacagc aatggaaatt atgatatcgt
    19081 aagaaaaaaa ttatctgaaa aaagattacc aaaatacaag caatatatta atttactcag
    19141 ataataatag tgactgattt aaaattcagc agcgtagaaa acaaatcaga gcacaaattt
    19201 cagccagaca atggagaaga taaaagatga ataaaaatat actttatata ttttctttat
    19261 taatcgctat tgttatttct tttatatttg aaaagaatgt aataagcaaa ataagtttta
    19321 attataataa aaaagaattc ttaattagtg atataacgaa ttttaattgg gattatgtaa
    19381 aactgtatat aatcaattca gatttccaaa aaattgtttt ttattataaa ggtaagatag
    19441 tttttgagga attaatagaa ctagatggag aagggaatgt tttacctcaa tatctatttg
    19501 attcagattt ggaaaatatt gaatattacg aatgtgatta caagaatggc aagatgcggc
    19561 ttttaaaaaa agaaaaatca catttttttg atggatattt ttattattat aaacctataa
    19621 attgcaggcc gaaacttttg taaaaccgtc catactcatt accgcctagt cggtaatgca
    19681 ttttcttagt agtatttgaa aaagtatgat gataaaaacg gtcaagccta actttttcat
    19741 aaaatccagc agagcaaggc agaaaaaaac acccaaattg gctataatcc cgacaaacac
    19801 actcaaggac aacaacatgg cagcctcgcc cgaagcaaaa ttcactgaag aaaagatttt
    19861 gtgggtcaaa caccacacgc cgaaactcat cactttcgcc atcagccgtc ccgaatccta
    19921 ccgttttaaa gccggacagt tctcccgact cggtttctac gaaggggaag gctttatttg
    19981 gcgtgcctac tccatcgttt ccgcagaata tgccgacacg ctcgaatatt ttgccgtact
    20041 catccaagac ggccccatgt cggcccgttt cgccaaaatg caacagggcg acaccatcct
    20101 gctcgataaa aatgccaccg gcttcctcct gcccgaacgc ttccccgacg gcaaggattt
    20161 ggtgatgctc tgcaccggct ccggcatcgc ccccttcctt tccattctcg aacaacccga
    20221 aatccggcag cgtttcgata ccgtcaacct gatacattcc gtatcttttc ccgaagaatt
    20281 gattttcaac gaccggctcg ccgcattgtc cgaacatccc ctggtaggcg aatacagaca
    20341 ctcgttccat ttcgtccctg ttaccacccg tgccgccaac ccctcgggct taagcggaaa
    20401 acgcattccg gaactcttaa aaaacagcag catcgaacag gcgctgcata ccaagttcac
    20461 cccggaatcc acacggttta tgatttgcgg caacccggaa atggtcaaag atactttcca
    20521 aacgctgctt gacatgggtt acgccatgca ccgcaaccgc attcccggtc aaatcatgat
    20581 ggaaaacggc ttctaaaaac caccctgctt gtccgatgcc ttcggatgga cggtcaaacc
    20641 gacacggcac gaaaaccgcg tcggcaaaaa tgccgtctga aaaaattcag acggcatctt
    20701 cggacacatt acctgcaaac ggcaacacat tccaacccaa accgattagg caatcaatac
    20761 gaacggctgt ttacatactt accggctttc atcaaccggt atcgatttaa ccgatttcct
    20821 taatattttt cctgtccgtt ttaaacttcg ccttaaacgc atccggtaaa tctttatcga
    20881 aataccaaag cccgtcaccc atttccaatg cgccgcccat tccatgcaga acaacttttt
    20941 cccccgttaa aggatcagtt tcggtaattt ctacctcatg catttttccc caatccaaaa
    21001 taggcaactt gtgtgcaaac gccaaaacct gttcgtcaga acggccgcac gccttatcgc
    21061 atcggcctga aacatatacg ggacaaggct catcctctgc ataaccgtcc ggaaggatac
    21121 cggctgcggc aggacacaat ccgtatgttt ccgcccacaa cgacaaagcc cgtctcgctg
    21181 cctttttatt gacaaataat ccggtaggcg gattgtccgc cacaccgttt ttcaaagccg
    21241 ctgttttcgc atccaacatg ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt
    21301 taagcagatt tttggcagat acgtaacaat ccgccccata atgcctgacc aattccgctt
    21361 taatcttata tgcatgcagg ctgcccaatg cgggaaaaaa acggactgcc tccgtattgc
    21421 accaatcaaa cgaggctttt ccggaatcca ataaagccga aacctcgcta tatacccgtt
    21481 caaacgtacc gatataattt attgctcccc cgccgtcgaa acaggcaagc acccccatac
    21541 cgtcaggcaa accgtacaac tgttccctca accgttcggg cagcgcggca ggcagcggtt
    21601 tcggattcat caaacggaaa cactgcctga tccactcctc gaccccgtgt tccgacagac
    21661 tgtattccaa ataatcacac aatgccgata catccgccat cgcacgatgc ctgtcttcca
    21721 caacaatccc caacctttcg atgatactgt ccaggctgtg cttgtaaaat tgcggataca
    21781 gacaccggga cagctgcaca ctgcacaaag caggcgatga aaatccgata cccgcacgat
    21841 gaaactcatg ctttaaaaac gtatagtcga aacggctgtt atgtgcaatc aacacacaac
    21901 ccttcaatac cgaaaacaac tcgccggcaa tctctgcaaa aacaggtgcg tcggcaacca
    21961 tgccgtctga aatccccgtc agccccgcca caaactgcgg aataggtttt tgaggattaa
    22021 ccagccactc atgccttacc acccttccct gctcaaactt gaccaaagcc acttcggtta
    22081 ccctgtcttc atacagattg ccgcccgtcg attccaaatc aaccacggca acaggcattc
    22141 caaaccgtaa aaataccttt tccagcaagg gccagcggga agcaacaatc attttattct
    22201 ctttaaattc aaacaacaaa ccgatatttt acacttttaa ggcatttcat ccaacaaaac
    22261 aattgacaga atccgatgat taccctaaaa ttcaaatctt tcttgcag
//
LOCUS       DAWKQJ010000016        20745 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000016 DAWKQJ010000000
VERSION     DAWKQJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.016"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(103..794)
                     /locus_tag="WJL20_000465"
                     /pseudo
     CDS             complement(103..794)
                     /locus_tag="WJL20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1053..1772)
                     /locus_tag="WJL20_000466"
     CDS             complement(1053..1772)
                     /locus_tag="WJL20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682657.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFVPIIAAAAF
                     GGALGTWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGGLAGNLAWR
                     PGIHALGFGANAANNAVNSRK"
     gene            complement(1899..2057)
                     /locus_tag="WJL20_000467"
     CDS             complement(1899..2057)
                     /locus_tag="WJL20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682658.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2054..2896)
                     /locus_tag="WJL20_000468"
     CDS             complement(2054..2896)
                     /locus_tag="WJL20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7682659.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPADERVLLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3131..4051
                     /gene="pyrB"
                     /locus_tag="WJL20_000469"
     CDS             3131..4051
                     /gene="pyrB"
                     /locus_tag="WJL20_000469"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ7682660.1"
                     /translation="MPNPLYKQHVISISDLTREQLELLLQTALKLKSEPHGNLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4061..4519
                     /locus_tag="WJL20_000470"
     CDS             4061..4519
                     /locus_tag="WJL20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7682661.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4584..5249
                     /locus_tag="WJL20_000471"
     CDS             4584..5249
                     /locus_tag="WJL20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682662.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5312..6529)
                     /locus_tag="WJL20_000472"
     CDS             complement(5312..6529)
                     /locus_tag="WJL20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7682663.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6671..7216
                     /gene="def"
                     /locus_tag="WJL20_000473"
     CDS             6671..7216
                     /gene="def"
                     /locus_tag="WJL20_000473"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ7682664.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7304..8230
                     /locus_tag="WJL20_000474"
     CDS             7304..8230
                     /locus_tag="WJL20_000474"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ7682665.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TVNEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRADVVAQQGTAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8309..9568
                     /gene="rsmB"
                     /locus_tag="WJL20_000475"
     CDS             8309..9568
                     /gene="rsmB"
                     /locus_tag="WJL20_000475"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ7682666.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9534..10133
                     /locus_tag="WJL20_000476"
     CDS             9534..10133
                     /locus_tag="WJL20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ7682667.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10133..12250
                     /locus_tag="WJL20_000477"
     CDS             10133..12250
                     /locus_tag="WJL20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ7682668.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPIPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            12243..13520
                     /locus_tag="WJL20_000478"
     CDS             12243..13520
                     /locus_tag="WJL20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ7682669.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13611..14798
                     /gene="dprA"
                     /locus_tag="WJL20_000479"
     CDS             13611..14798
                     /gene="dprA"
                     /locus_tag="WJL20_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ7682670.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14824..15285
                     /locus_tag="WJL20_000480"
     CDS             14824..15285
                     /locus_tag="WJL20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ7682671.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15357..17663
                     /gene="topA"
                     /locus_tag="WJL20_000481"
     CDS             15357..17663
                     /gene="topA"
                     /locus_tag="WJL20_000481"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ7682672.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGGHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17736..18431)
                     /locus_tag="WJL20_000482"
     CDS             complement(17736..18431)
                     /locus_tag="WJL20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682673.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLIICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18451..18804)
                     /locus_tag="WJL20_000483"
     CDS             complement(18451..18804)
                     /locus_tag="WJL20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682674.1"
                     /translation="MEFLSLYPWWSFFIILTIILSYIGFCAHLHIQNIVWGKLLLSTI
                     VWAILAISFGLASIMGNSKRKKYERRDRHTERNEKAFIGTLLVGFGLSINLLNLCCLH
                     KDFSTPSKNIEVNRS"
     gene            18922..19299
                     /locus_tag="WJL20_000484"
     CDS             18922..19299
                     /locus_tag="WJL20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7682675.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19388..19957
                     /gene="rsmD"
                     /locus_tag="WJL20_000485"
     CDS             19388..19957
                     /gene="rsmD"
                     /locus_tag="WJL20_000485"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ7682676.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVRETL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20080..20331
                     /locus_tag="WJL20_000486"
     CDS             20080..20331
                     /locus_tag="WJL20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ7682677.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20428..20511
                     /locus_tag="WJL20_000487"
     tRNA            20428..20511
                     /locus_tag="WJL20_000487"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20462..20464,aa:Tyr,seq:gta)
     gene            20542..20615
                     /locus_tag="WJL20_000488"
     tRNA            20542..20615
                     /locus_tag="WJL20_000488"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20574..20576,aa:Gly,seq:tcc)
     gene            20625..20699
                     /locus_tag="WJL20_000489"
     tRNA            20625..20699
                     /locus_tag="WJL20_000489"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20657..20659,aa:Thr,seq:ggt)
BASE COUNT         5172 a         5302 c         5656 g         4615 t
ORIGIN      
        1 gctgaagcac taagtgaatg ggtttcgtac tatttgtact gtctgcggct tcgtcgcctt
       61 gtcctgatta ctatataaaa taaatgggca aaaatcggtt tattatcgtt tttgccgcat
      121 ttggatttgt tctaccgtaa aacgtgtttg acgaacggga ttcttattaa aaaacatctg
      181 atttctaaca aaatcagtat tttttggcac gatggctaaa atttttcctt ccatttcgcc
      241 atcacgtgtt ttccatgcgc tcaagaattg tgatttgctc attgagacgt gccccagcga
      301 tggatcagcc agcaaaacag tttctccgtt aataccgttc aataccgaaa aatggttgtt
      361 tttacggtat tttaaataca caattacagg aatttttagt tgtaccaact gttcaaatgg
      421 caaagcataa ccttgtgctt caaaacccag ttcgggcatt atgcgttgca tatcatcaaa
      481 agaagcacgc atttgggttt tatccatttt gtctaagatt tccgcttcag aataatgtct
      541 gccataaaaa ttattcagta acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc
      601 ttgttttact atgccggaat ctcgccgtgc tttccaactt cgtacatgga tgttttggta
      661 agaagcgggg ggtcaacaaa cataggccaa gcaaaaacta tatttggggc gaaaccaatc
      721 aaagccgcat aatttatcaa tttataaaga ttttttatca taatatgtat acgcggaata
      781 aacgcatgaa acataaaaaa ataaaatcat atgcaatttt attgcatgaa taaatatgaa
      841 taaaatagat aatgatggga gtaaatacgc catgtatttt ggaagtttaa atttattaat
      901 aataaaataa tttatcgtag cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat
      961 taatacaccc atcatcatgc aaactctata ttaagaaata gctaactaat gctattgtgg
     1021 aaattagtta gctaactaaa agttattaat gattattttc gagaattgac tgcattatta
     1081 gcagcattgg caccaaaacc tagtgcatga atacccggtc tccatgccaa attcccagcc
     1141 aatccgcctc cggcagcagc agccagccct gttgccgcta ctcctgttgc cgcaccgatt
     1201 cctgccgcag tagccgcgcc ttgcgcagtt cctaatttac catgattata caaattagca
     1261 ccatgatacc cccatgtacc taatgcaccg ccaaaagcag cggctgcaat aatgggaaca
     1321 aattcacctt gtgtttcttt catttcagcc tgtgataatt gaattgcttt cacattttgg
     1381 ctgtcaaaaa cttggctgtc taaattttgc gccattacag gtgtaatcat catagccatt
     1441 acagttgcaa ttttcgttgc gctggtttgc acataaatag gattagcaaa ttcgctttga
     1501 ttgcgttcag tgttgatgta gctaatactg ctttctagtt tgaatttacc cttgtcagtc
     1561 aataattctt ccaaacttaa aggtaagtca gcataagcaa tttggctggc aacagtcaaa
     1621 ataaaatcta ttagacattt gtgtttttgc atcatttcgt ttgattttct agtttttgag
     1681 aatgatacaa agttttttac aaagtaaaga gtcactctga aaaacatttt ttcattataa
     1741 atcaaaatat tgatagaata aatagcgagc atcgattcac ggtgtacttt agtgcaaagc
     1801 gtacaacgcg agcctgaacc accagccgca acaggaaaag aaagccgata gagtgcaatg
     1861 cttgccaacg tgcaagcgag cttgcaagaa cgcttggctc aacgagagca ggcaagacag
     1921 aaagcagaaa gcaggatagg agcggtaacg caaaggtctc gggctttgat ttcgccgtaa
     1981 accctgctgc cgccttgtcc ggaaagggtg caggcggcga gtgccgacag ggtgcagatg
     2041 gggagggggg ttttcatttg gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt
     2101 ttttgaaata caggcggtgc gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga
     2161 ttttggttac ccctgtttcc gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt
     2221 agcctttgcg gcggctttgc ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc
     2281 tggcgagggt gcaggcttcg cggaagccgc agacggcgac ggcattgtgt ttgccttctt
     2341 caaaaatacc cagcaggcgg tagccttggg ggcgttggac tttgttgatc tgttcggtaa
     2401 agcggttgat gtcggtcagg gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg
     2461 tgtcgtccgc gccgatttcg cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg
     2521 ctttctcggc ggcgtgtttt tcttcgattg cctgtgccag caggacgcgc tcgtcggcag
     2581 ggtcgttttg tccgccctgt tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca
     2641 ggtcgttgtc ggcggcaaag tccatcagga agcggaacat ctgcggattg tcggtttcca
     2701 gttttttatc gacggcgacg cagcggatgt tgtccaccag tatggggcgc acccatttcg
     2761 aataggaaag cctgtggtct ttggtgaacg aggacagaat gccgcacagg cgttccgccc
     2821 agtcgctggg acggaaaatc ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg
     2881 ggttgcagac taacatggcg gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg
     2941 cttatgcggg cgtgtttgga tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt
     3001 tgcggcggta aatgtgcgat aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca
     3061 aacatacggg cggcgtggac gtataaccgg atgcctgctt ggcgcgggtc ttttacggag
     3121 gaggggtgaa atgcctaatc cgctatataa acagcatgtt atttctattt ctgatttgac
     3181 gcgggaacag ctcgagctgc tgcttcagac ggcattgaaa ctgaagtcgg agccgcacgg
     3241 caacctgttg gaagggaaac ttatcggttc gtgcttcttt gaaccgtcca cgcgtacgcg
     3301 cctgtcgttt gaaacggcgg tacagcggct gggcggcaag gtcatcggct tttcggacgg
     3361 cgcgaatacc agtgccaaaa aaggcgagac gcttgccgat accgcccgca tcatttccgg
     3421 ctacaccgac gcaatcatcc aacgccatcc caaagacggc gcggcgcgcg tggcggcgga
     3481 gttttcgcgc gtccccgtta tcaacgccgg cgacggcacg aaccagcacc ccagtcagac
     3541 gctgctcgac ctggttacca tttatgaaac gcagggacgt ttggacaagc tcaaaatcgc
     3601 catggcgggc gacttgaaat acggacgtac cgtgcattcg ctttgtcagg cgttgaaacg
     3661 ctggggctgc gaatttgcct ttgtttcgcc gcccagcctc gcgatgcccg actacattac
     3721 cgaagagttg gacgaagccg gctgccgata ccgtatcctc ggtagtttgg aagaagcggc
     3781 ggaatgggcg gacatcctgt atatgacccg tgttcaacgc gaacgtttcg acgaacagga
     3841 atttgccaaa atccaaggca aattcaatct cgacgcatcc atgcttgccc gcgccaaacc
     3901 gaacctgcgc gtgctgcacc ctttgccgcg cgtggacgaa atccatcccg atgtcgatgc
     3961 tacgccgcat gcctattatt tcgagcaggc gaccaacggc gtttatgcgc gtatggcgat
     4021 attgtcgctg gtgttgaacg aagaagtgtg aggaaccgat atggaaaccc cgaaactcag
     4081 tgtcgaagcc attgaaaaag gtacggttat cgaccatatt cccgccggca gggggctgac
     4141 catcctgcgc cagttcaaac ttttgcacta cggcaacgcg gtaaccgtgg gcttcaacct
     4201 gcccagcaaa acccaaggca gcaaagacat catcaaaatc aaaggcgtgt gcttggacga
     4261 caaagccgcc gaccgcctcg ccctgttcgc ccccgaagcg gtggtcaata ccatcgacaa
     4321 tttcaaggtc gtgcagaagc ggcatttgaa cctgcccgat gaaattgccg aagtgttccg
     4381 ttgcccgaac acgaattgcg ccggccacgg cgagccggtc aaaagccggt tttatgttaa
     4441 aaaacacaac gggcagacgc ggctgaaatg ccattactgc gaaaaaacct acagccggga
     4501 ttcggtggcg gaagcctgac ggattccctt aaaccgagcg ggcggcattt cgtctgccgc
     4561 ttgttttgcc aatctgaaat ggaatgatga tgcacgcttc tgtccaaagc cgtttcgcac
     4621 cgatacttta tgttttgatt ttctttgccg gttttttgac cgcgcaaatt tggttcaatc
     4681 agaaagccta tactgaagag ctgcctccgc ttctgtccgc attgtccgcc gtcgcgctgg
     4741 tgtggctggc gtgggcgttc gtgtcggcgc gttcaaaggc taaggcggaa aagttctacc
     4801 gcgaaaaaat gatacagaac gaaagcatac accccgtcct gcacgcttct ttgcaacact
     4861 tggaacacaa gccgcaaatg ctcgccctgc tggtcaaaaa ccacggcaaa gggatggcgg
     4921 aacaggtcag gttcaaggcg gaagtgctgc ccgacgacga agacgcgcgc acgattgccg
     4981 ccgagttggc aaaaatggat atgtttgcat tggggacgga cgcggtcgcc tcgggcgaaa
     5041 cctatggacg cgtgttcgcc gatattttcg agttgtcggc ggctttggaa gggcgcgcgt
     5101 tcaaaggaat gttgaaactg acggcggaat ataaaaacat cttcggcgat gcctgccgtt
     5161 cggaaacggc gttggatttg ggcgcgctca atcaggcgtt gcaggagatt tcaaaaacat
     5221 cggaaaagtc caaacggata ttttattgaa gatggaaaaa tgccgtctga aacggaaggt
     5281 gtttcagacg gcatttttgt cggatgatta attattcgga gcggtcgaag ccaaacttca
     5341 cgcggctgcg gccctgatcc ggtatattgt ccaaatcgcg tcccggattg gcggcggtgt
     5401 cgcctacgga aatatcggag atgttttcca aaatgatggc ggacgacagg tgttcggagg
     5461 tgcggtaaac cattgccaag cccacttctt cggcaggagt ggaaatcagc tcgacggtat
     5521 ccctgctttt gaaattgttg gagaggtcga cctgcatcgt tttcttgcgt ttgtagaggc
     5581 tcaaaaccgt gcctttgtcc aaaccgtccg cctcgccttt gtcgatggtg atggtttgaa
     5641 actggccggc aatccttgta ccttcaaaca cggaaacgat tttagcctga accgggcggg
     5701 acggttcgtg cggcatcatg ttgaagcggt cggtgtcttc cggcattttc atcaggtagt
     5761 cgccctgctg tatttcggaa atggcggttt cgaccaccag cggctgtatc gaaggggtgc
     5821 gcagcggggt aatcaaagga tgggtgcggg tatggtattc gttgtctttc ggccgttctc
     5881 cagcctgttt cgagcgttgt tcgaggacag agtcggtata gtcgagggag cgcacgatgc
     5941 cgctgaatgc gacttcctgc ccgaggaatt tacccgtatc cggatcggtg atgtttttat
     6001 tgattcggta ggtcaggtag cggcccggct ctttcaggcc tttggtgtaa accctggtgc
     6061 ctttggtgta cagcagcctg ccttccgggc ccgagagcag gcgcggcgcg gcagcggttt
     6121 ctttgcggga aacgatttgc ggatgccgca taaagatgcg gtagaagttg acatcgatgg
     6181 cgggaatacc gtatccggac acttccttat ccggactcat tttgacgacg gggatgccgt
     6241 ctgtctgttc caagccgagg cgcggttcgc cgtcaacgtg gcgcaacacc aatacctggt
     6301 ccggataaat caggtcggga ttgtggattt gatcccggtt cgcgtcccac aggcggctcc
     6361 attgccacgg gctgtacagg tatttgcccg aaatgcccca cagggtgtcg ccctgtttga
     6421 ccgtgtagcg ttccggcgcg ttcgggcgca cctccaaatt tgccgccaaa gtttgtgttg
     6481 agaatgccat acctgccgcg cagagcaggg ttataatacg acgttgcata accgttcccc
     6541 ttatctgata aatttcggtt tgtcttgctt gattgggttg gaaaaagcgg cggcagcccc
     6601 tcgggatgtg ccgcgtgata aaaaatgttc cgcattttaa catcgaatta tccgcaccat
     6661 cacggtaatt atgaaaaaca ggcggcgtat ccgccgaagg aaagagaaaa ttatggcttt
     6721 attgaatatc ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca
     6781 agtcgacgag cgcatccgga agctgattgc cgatatgttt gaaacgatgt acgaatcgcg
     6841 cggcatcggg ctggcggcga cgcaggtcga tgtgcacgag cgcgtggtcg tgatggattt
     6901 gaccgaagac cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg
     6961 cgaaaccact tacgaagagg gctgcctgtc cgtgccgggc atttacgaca ccgtaacccg
     7021 cgccgaacgc gtcaaggtcg aggctttgaa cgaaaaaggc gaaaagttca cgctggaggc
     7081 ggacggcttg ctggcgattt gcgtgcagca cgagttggac cacctgatgg gtatcgtgtt
     7141 tgtcgaacgc ctttcccaac tcaagcaggg gcggattaag accaagctga aaaaacgtca
     7201 gaaacatacg atttgaccct tttgccgtgc cgtctgaacg ctgcaaagtt tttcagacgg
     7261 cacggtcttg tccgacaatt ttacgcacac gcaggaacac gctatgaaag tcatcttcgc
     7321 cggcacgccc gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat
     7381 cccgctggtg ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgactgcccc
     7441 gcccgtcaaa caagccgcgc tggaactcgg tttgcgcgtc gaacagcccg aaaagctgcg
     7501 caacaacgcc gaagccctgc aaatgctcaa agaggtcgag gcagacgtaa tggtggttgc
     7561 cgcctacggt ttgattctgc cgcaagacgt gttggatgtg ccgaaacacg gctgcctcaa
     7621 tatccacgcc tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga
     7681 ggcaggcgat gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg
     7741 cgatgtggtc agcgaacacc gctacgccat ccaaccgacc gataccgtca acgaagtcca
     7801 cgacgcgctg atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag
     7861 caaaggccgt ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact
     7921 aagcaaagaa gaggcgcgta tcgattggag cgaaagtgcg gcggttatcg aacgcaaaat
     7981 ccgcgccttc aaccccgtgc ctgccgcgtg ggttgagtat cagggcaagc cgatgaaaat
     8041 ccggcgggcg gacgtggtgg cgcaacaagg cacggcaggc gaagtgttgt cctgttcggc
     8101 ggacggtttg gtcgttgcct gcggcgaaaa cgcgctgaag attaccgaat tgcagcctgc
     8161 cggcggcagg cgtatgaata tcgcggcgtt tgcagcagga cggcatatcg aagcagggac
     8221 gaagctgtaa atcccttcag acggcattcc gatccgcaaa cgggaatgcc gcctgaaacc
     8281 atcagttgaa gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc
     8341 cgacagcatt gcggcggttg ccgaaggacg caaccttcag gacgtgttgg cgcaaatccg
     8401 caccgcgcat cccgacctta cggcgcagga aaacggcgcg ttgcaggaca tcgcctacgg
     8461 ttgccagcgt tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaaaccgat
     8521 tgacaatccg cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg
     8581 caacgcgccg cacgccgtgg tcaatgaggc ggtggaaagc atagccaaaa tcgggcgcgg
     8641 gcagtaccgt tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gcgacaagct
     8701 cgcggcttcc tgtaaaaaag acgatgtggc gaaacacaac ctgccgctgt ggtgggtggc
     8761 ttacctgaaa aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca
     8821 tccgccgatg acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa
     8881 actggtggcg gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga
     8941 agccgtgccg gtaaaccgtc tgcccggttt ttcagacggc attgtttcgg tacaggactt
     9001 cggcgcgcag caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc
     9061 gtgcgccgcg ccgggcggca agacggggca tattttggaa ctggcggatt gccgcgttac
     9121 cgccttggac atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg
     9181 ctttcagacg gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg
     9241 aaagcctttc gacgcaatcc ttgccgacgt gccatgcacc gcttccggcg tggcgcggcg
     9301 caatcccgac gtgaaatggc tgcgccgtcc gaccgacgcg ctaaaaaccg cccgccagca
     9361 ggaagccctg ctggacgcac tttggcagac gctgaaacag ggcggcagaa tgctgcttgc
     9421 cacctgttcc gtgttcgtcg aagaaaacga cgggcaattg caaaaattcc tcaaccgcca
     9481 tgccgatgcc gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt
     9541 ttattacgcg cttattcaaa agcattaaac aatggcttgt gctgctgccg atgctttccg
     9601 ttttgccgga cgcggcggcg gaggggatag atgtgagccg cgccgaagcg aggataaccg
     9661 acggcgggca gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac
     9721 aggcgttgcg ccggggcgtg ccgctcaact ttaccttaag ctggcagctt tccgccccga
     9781 taatcgcttc ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact
     9841 acaaactgag tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcgttttcga
     9901 cagactacga caccttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag
     9961 tcctgaacaa aggcgcgctg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc
    10021 tgacgctgtc cacttcaaaa ctgcccaagc catttcaaat caatgcattg acttctcaaa
    10081 actggcattt ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg
    10141 ttttctaccg atcgcagcca tatgcgccgt cgtcctgttg tacggactga cggcggcaac
    10201 cggcagcacc agttcgctgg cggattattt ctggtggata gttgcgttca gcgcaatgct
    10261 gctgctggtg ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg
    10321 cgacggcgta ttcggttcgc agattgccaa acgcctttct gggatgttta cgctggttgc
    10381 cgtactgccc ggcgtgtttc tgttcggcgt ttccgcacag ttcatcaacg gtacgattaa
    10441 ttcgtggttc ggcaacgata cccacgaggc gcttgaacgc agcctcaatt tgagcaagtc
    10501 cgcattgaat ctggcggcag acaacgccct tggcaacgcc atccccgtgc agatagacct
    10561 catcagcacc gcctccctat ccggcaatat gggcagggtg ctggaacatt atgccaacgg
    10621 gtttacacag ttgtcccttt acaatgccgc aagcggcaaa atcgaaaaaa gcatcaaccc
    10681 gcacaagctc gatcagccgt ttccggataa ggcgcgttgg gaaaaaatcc aacaggcggg
    10741 ttcggtcagg gatttggaaa gcataggcgg cgtattgtac gcgcagggct ggctgtcggc
    10801 gggtacgcac aacgggcgcg attacgcctt gtttttccgc cagccgattc ccaaaggcgt
    10861 ggcagaggat gccgtcttaa tcgaaaaggc aagggcgaaa tatgctgagt tgagttacag
    10921 caaaaaaggt ttgcagacct ttttcctggc aaccctgctg attgcctcgc tgctgtcgat
    10981 ttttcttgca ctggtcatgg cactgtattt cgcccgccgt ttcgtcgaac ccgtcctatc
    11041 gcttgccgag ggggcaaagg cggtggcgca aggcgatttc agccagacgc gccccgtgtt
    11101 gcgcaacgac gagttcggac gcttgaccaa gttgttcaac cacatgaccg agcagctttc
    11161 catcgccaaa gaagcagacg agcgcaacag gaggcgcgaa gaagccgcca gacattatct
    11221 tgaatgcgtg ttggaggggc tgaccacggg cgtggtggtg tttgacgaac aaggctgtct
    11281 gaaaaccttc aacaaagcgg cggaacagat tttggggatg ccgcttaccc ccctgtgggg
    11341 cagcagccgg cacggttggc acggcgtttc ggcgcagcag tccctgcttg ccgaagtgtt
    11401 tgccgccatc ggcgcggcgg caggtacgga caaaccggtc catgtgaaat atgccgcgcc
    11461 ggacgatgcc aaaatcctgc tgggcaaggc aaccgtcctg cccgaagaca acggcaacgg
    11521 cgtggtaatg gtgattgacg acatcaccgt tttgatacac gcgcaaaaag aagccgcgtg
    11581 gggcgaagtg gccaaacggc tggcacacga aatccgcaat ccgctcacgc ccatccagct
    11641 ttccgccgaa cgtctggcgt ggaaattggg cgggaagctg gatgagcagg atgcgcaaat
    11701 cctgacgcgt tcgaccgaca ccatcgtcaa acaggtggcg gcattgaagg aaatggtcga
    11761 agcattccgc aattatgcgc gttccccttc gctcaaattg gaaaatcagg atttgaacgc
    11821 cttaatcggc gatgtgttgg cattgtatga agccggtccg tgccggtttg cggcggagct
    11881 tgccggcgaa ccgctgatgg tggcggcgga tacgaccgcc atgcggcagg tgctgcacaa
    11941 tattttcaaa aatgccgccg aagcggcgga agaagccgat gtacccgaag tcagggtaaa
    12001 atcggaagcg gggcaggacg gtcggattgt cctgacggtt tgcgacaacg gcaaagggtt
    12061 cggcagggaa atgctgcaca acgccttcga gccgtatgta acggacaaac cggcgggaac
    12121 gggattgggt ctgcccgtgg tgaaaaaaat cattgaagaa cacggcggcc gcatcagcct
    12181 gagcaatcag gatgcgggcg gcgcgtgtgt cagaatcatc ttgccaaaaa cggtagaaac
    12241 ttatgcgtag cagcgatatt ttaattgtag acgacgaaat cggcatccgc gacctgctgt
    12301 cggaaatcct gcaggacgaa ggttattcgg tcgcattggc ggaaaacgcc gaagaggcgc
    12361 gcaagctgcg ccatcaggcg cgtccggcga tggtgctgct ggatatttgg atgcccgatt
    12421 gcgacggcat cacccttttg aaggagtggg cgaaaaacgg gcagctcaat atgccggtgg
    12481 tgatgatgag cgggcacgcc agcatcgata ccgccgtgga agccaccaaa atcggcgcga
    12541 tcgatttttt ggaaaaaccg atttccctgc aaaagctgct gtctgccgtc gaaaacgcgt
    12601 tgaagtacgg tgcggcgcaa accgaaacgg ggcctgtatt cgacaagctg ggcaacagtg
    12661 cggcgattca ggaaatgaac cgtgaggtag gggctgcggt gaaacgtgcc tctcccgtac
    12721 ttttgacggg cgaggcaggt tcgccgtttg aaacggtggc acgctatttc cataaaaacg
    12781 gtacgccgtg ggtcagcccg gcaagggtcg aatatctgat cgatatgccg atggaactgt
    12841 tgcagaaggc ggagggcggc gttttgtatg tcggcgacat cgcccagtac agccgcaaca
    12901 tccaagccgg tattgccttt attgtcggca aggcggaaca ccgccgcgtc agggtggtcg
    12961 catcgggcag cagggcggca ggttcagacg gcattgcctg cgaggaaaag ctggcggaac
    13021 tgctgtcgga gtcggtcgtc cgcattccgc cgctgcgtat gcagcatgaa gatattccct
    13081 tcctgataca ggggattgcc tgcaatgtgg cggaaagtca aaagattgcg cccgccgcat
    13141 tcagtgaaga ggcacttgcc gcattgaccc gttacgactg gccgggaaat ttcgaccaac
    13201 tgcaaagcgt cgttgcaacg ctgttgttgg aggcggacgg acaggaaatc ggcgcggcgg
    13261 cggtttcttc ccttttgggg cagaatgtgc ctgccgaggg agcggaagat atggtgggcg
    13321 ggtttaattt caacctgccc ctgcgcgaat tgagggagga ggtggagcgg cgttatttcg
    13381 agtaccacat cgcccaagaa ggtcagaata tgagcaaagt ggcgcagaag gtcggtttgg
    13441 aacgcacaca cctttaccgc aaactcaaac agctcggcat cggcgtttcg cgccgggcgg
    13501 gggaaaaaac cgaagaatag gcccggacgg tcggttttcc ggctgcgggc ttttgttttc
    13561 agacggcatt tggtgcaaat gccgtctgaa atcgtaaggg gacggatttt atgacagagg
    13621 acgaacgttt cgcgtggttg caactggctt ttacgcccta tatcggtgcg gaaagtttct
    13681 tgctgctgat gcgccgtttc ggcagcgcgc aaaatgcctt gtccgcaccg gcggaacagg
    13741 tcgccccggc ggtgcggcac aagcaggcgg ccgaggcttg gcgcaatgcg gaaaaacgcg
    13801 ctttggcgcg gcaggcggca gaagcggcgt tggaatggga aatgcgggac ggatgccgcc
    13861 tgatgctgct tcaggatgaa gatttccccg aaatgctgac gcaggggctg accgcgccgc
    13921 cggttttgtt tttgcgcggc aacgtgcggc tgctgcacaa gccttccgcc gccatcgtcg
    13981 gcagccgcca tgccacgccg caggcgatgc ggattgccaa agatttcggc aagtcgttgg
    14041 gcgggcaaaa tatccccgtt gtgtcgggta tggcttccgg tatcgatact gccgcccatc
    14101 agggcgcact ggaggcagaa ggcggcacca tcgccgtgtg ggggacgggt atagaccgca
    14161 tttacccgcc gtccaataaa aaccttgcct atgaaatcgc cgaaaaagga ttgattgtca
    14221 gcgagtttcc catcggtacg cgcccgtatg ccggcaactt tccgcgccgc aaccgcctga
    14281 ttgccgcgct gtcgcaggtt acgctggtgg ttgaagctgc gttggaatcc ggttcgctga
    14341 ttaccgccgg cttggcggca gaaatggggc gcgaagtgat ggcagtaccc ggttcgatag
    14401 acaatccgca cagcaaaggc tgccacaaac tgattaaaga cggcgcgaaa ttggtggaat
    14461 gcctggacga catcctgaac gaatgcccgg ggctattgca aaatacgggt gcttcatcat
    14521 attctataaa taaggatacg cccgacacgg gacgccgcgc cgttcagacg gcatacgccc
    14581 cgccgcctgc cgcaaaaatg ccgtctgaag cggcggcagg cggcacagcc gtcggcggca
    14641 tactggataa aatgggtttc gaccccatcc atcccgacgt gcttgccgga cagttggcta
    14701 tgcctgccgc agatttgtat gccgcactgt tggaattgga attggacggc agcgttgccg
    14761 caatgcccgg cggcagatac cagcgtatcc gaacttgaac gcactttata ttaaggaaca
    14821 cgaatgaccg aagtcatcgc ctacctcatc gaacatttcc aagatttcga tacctgtccg
    14881 ccgcccgaag acttgggtat gctgcttgaa gaagcgggtt tcgatacgat ggaaatcggc
    14941 aacaccctga tgatgatgga agtattgctc aacagctccg aattttccgc cgaacctgcc
    15001 gacagcggcg cattgcgcgt gtacagcaaa gaagaaaccg acaacctgcc gcaggaagtg
    15061 atggggctga tgcagtatct gattgaagaa aaagccgtca gctgcgaaca gcgggaaatc
    15121 atcatccacg cgctgatgca catccccggc gacgaaatta ccgtagatac cgccaaagtg
    15181 ctgaccctgc tgcttttatg ggcaaacaag agcgagctgc ccgtgttggt cggcgacgag
    15241 ctgatgagcg cgcttttact cgacaacaaa cccacgatga actgaagcgg cttcagacgg
    15301 cccgcccgag gccgtctgaa acgccggcat caaaaccacc atccagagaa cgacaaatgg
    15361 cgaaaaacct attaatcgtc gaatccccgt ccaaagccaa aaccctgaaa aaatatttgg
    15421 gcggcgattt tgaaatcctt gcatcctacg gacacgtccg cgacctcgtc cccaaaagcg
    15481 gcgcggtcga tcccgacaac ggctttgcga tgaaatacca actcatcagc cgcaacggca
    15541 aacacgtcga tgccattgtc gccggtgcca aagaagctga aaacatctac ctcgccaccg
    15601 acccggatag ggaaggcgaa gccatttcct ggcatctttt ggaaatcctc aaatccaaac
    15661 gcggcttgaa aaacatcaag ccgcagcgtg tcgtgttcca cgaaatcacc aaaaacgccg
    15721 tgctcgatgc cgttgcccat ccgcgcgaaa tcgaaatgga cttggtcgat gcacaacaag
    15781 cccgccgtgc tttggactat ttggtcggct ttaacctttc gccattgtta tggaaaaaaa
    15841 tccgccgcgg tttgagcgca ggccgcgtgc aaagccccgc tttgcgcctg atttgcgaac
    15901 gcgaaaacga aatccgcgcg tttgaagcgc aggaatattg gacggtgcat ctcgacagcc
    15961 acaaaggccg cagcaagttc accgccaaac tcgcccaata caacggcgcg aaactcgaac
    16021 aattcgacct gtcgaacgaa gccgcgcaag ccgacgtatt gaaagaactc gaaggcaaag
    16081 aagccgtcgt taccgccatt gaaaagaaaa agcgcagccg caatcctgcc gcgccgttca
    16141 ccacttctac catgcagcag gatgccgtgc gcaaactcgg cttcaccacc gaccgcacca
    16201 tgcgtaccgc ccaacagctt tatgaaggta tagacgtagg gcagggcgcg atcggtctga
    16261 ttacctatat gcgtaccgac agcgtgaatt tggcggatga agcgttaacc gaaatccgcc
    16321 attacattga aaacaaaatc ggcaaagaat atctgccgag tgccgccaaa caatacaaaa
    16381 ccaaatccaa aaacgcccaa gaagcccacg aagccatccg cccgacttcc gtgtaccgca
    16441 cgcccgaaag cgtcaaaccc ttcctgagcg ccgaccagtt caaactctat caaatgattt
    16501 ggcagcgcac cgtcgcctgt cagatgacgc ccgccaaatt cgaccaaacc accgtcgata
    16561 ttaccgtcgg caaaggcgta ttccgcgtaa ccggacaagt gcaaaccttc gcaggcttcc
    16621 tcagcgttta cgaagaaagc agcgacgatg aagaaggaga agacagcaaa aaactgcccg
    16681 aaatgagcga aggcgacaaa ttgcccgtgg acaaactcta cggcgaacaa cactttacca
    16741 ctccgccgcc acgctacaac gaagccacgc tggttaaagc cctcgaagaa tacggcatcg
    16801 gccgcccctc gacctacgcc agcatcatct ccacgctcaa agaccgcgaa tacgttaccc
    16861 ttgagcaaaa acgcttcatg cccaccgaca caggcgacat cgtcaacaaa ttcctgaccg
    16921 aacacttcgc ccaatacgtc gattaccact tcaccgccaa actcgaagac cagcttgacg
    16981 aaattgccga cggcaaacgc caatggattc ccgtgatgga caaattctgg aagccgttta
    17041 tcaagcaagt ggaagaaaaa gagggcatcg aacgcgccaa atttaccacg caggaactcg
    17101 acgaaacctg ccccaaatgc ggcggacaca aactgcaaat caagttcggc aaaatgggtc
    17161 gcttcgtcgc ctgcgccggc tatcccgagt gcagctacac gcgcaatgtc aacgaaaccg
    17221 ccgaagaagc tgccgaacgc atcgccaaag ccgaagccga acaggcagaa ctcgacggac
    17281 gcgagtgccc gaaatgtggc ggtcgcctag tgtacaaata cagccgcacc ggcagcaaat
    17341 tcatcggctg cgccaactat ccgaaatgca aacacgtcga gccgctggaa aaaccgaaag
    17401 ataccggcgt ccagtgtccg caatgcaaaa aaggcaacct cgtcgagcgc aaatcccgct
    17461 acggcaaact gttttacagt tgcagcacct atcccgactg caactacgcc acttggaacc
    17521 cgcccgttgc cgaagaatgc ccgaactgcc attggccggt cttgaccatc aaaaccacca
    17581 aacgctgggg cgtggaaaaa gtttgtccgc aaaaagaatg cggctggaaa gaacagattg
    17641 aaccgcccgc gccgaaggag taagattagg ttggtttgaa agagaaaagg tcgtctgaaa
    17701 aattttcaga cgacctttgc ttttctgtga ttggtttatt tgaatccgcg tgttgtttta
    17761 aagtccgata aaatccggtt catttcaggc gcaaacaagg cgatgtaatc gtaagataga
    17821 ccgcgactgg cactgggatg gggaaagcag acgacttcgc aatcttcaaa cgattggaat
    17881 ttgacattga aacgtgtacc gtcaaattct ttttgcaccg tctccagcgg tttggtctgc
    17941 ttaccgacca actgctcgaa gcgtggcagt acattttggt tgttcagaaa atccgccaac
    18001 ctgctgccca tgaagaggat gactttcgga cgcagttttt cgatgtggta gagaaaatta
    18061 tcgatgtgct cgggttgtgt gaacttgtcg ggattgtcga tagtgttgcc ctgtgtagca
    18121 gcccagttgg tttgaaccag ggatttttca aatgcaccgc ccaatccatt ttcgtctaag
    18181 gggtgtcccc acatttcaaa ccaatttttt atcgtattgt cgtaacgcca cttttttgcc
    18241 tgctctccga aatagaggga tttgtttgca aatgtatggt cgattttgtt ttcagggagt
    18301 ttgtattcac ctgctacata agcagcctca tcggctttac tccaacccca ttcataccca
    18361 caaattatta aaccatgttt gtcattgtag cctttgaaca gactgttgct caaattcaaa
    18421 tccttcatca tgaacccttc cttttaaaat ttaagagcga ttgacttcaa tgtttttaga
    18481 tggggtggaa aaatccttgt gtaggcaaca taaatttaat aaatttattg ataatccgaa
    18541 acctactaat agcgtaccta taaaagcttt ttcattacgt tcagtatgac ggtcacgtcg
    18601 ttcatatttt ttacgcttgc tgttccccat tattgaagct aagccaaatg atatggcgag
    18661 aattgcccaa acaattgtac ttaataacaa ttttccccat actatatttt gaatgtggag
    18721 atgtgcacag aatccaatat agctaaggat aatagttaat ataataaaaa aagaccacca
    18781 agggtaaaga gataggaatt ccatgttttc ctttttttgt aaaaaggaaa aaattttatc
    18841 taagagttaa ttgcctaacc agccagaaga agtttaaaat ctatcccaat aattcaacca
    18901 tctatacaga aagttcagct tatggaaacc cacgaaaaaa tccgcctgat gcgcgaattg
    18961 aataaatggt cccaggagga tatggcggaa aagctggcga tgtcggcagg cgggtatgcc
    19021 aaaatcgaac ggggcgaaac gcagttaaat atcccgcgtt tggagcagtt ggctcagatt
    19081 ttcaaaatcg atatgtggga cttgctcaaa tcgggcggtg gtgggatggt gtttcagatt
    19141 aatgaaggtg atagtggtgg cgatattgcg ttgtatgcgt cgggtgatgt ttcgatgaaa
    19201 atagaatttt taaaaatgga gttgaaacac tgcaaagaaa tgttggaaca aaaagacaaa
    19261 gaaatcgagc tgctccgcaa gctgaccgaa accgtttaaa cagatatgcc gtctgaaaaa
    19321 agttttcaga cggcatattc tttgacaggt cttgtataat accgtttgaa cttacaggtt
    19381 tttgattatg gcggcaggca aacataccaa acacagcaac cgggtacgca ttatcggcgg
    19441 gcaatgccgg ggcaggaaat tgagttttac atccgccgac ggactgcgtc cgacacccga
    19501 cagcgtgcgt gaaacgctgt ttaactggct gggacaggat ttgacgggta aaacggtttt
    19561 ggatctcttc ggaggcagcg gcgcactcgg tatagaagcc gcttcgcgca acgccaaacg
    19621 cgtgctgatt tcggataaca accgccaaac cgtgcagacc ttgcagaaaa acagtcgcga
    19681 actgggtttg gggcaggtgc aaatcgtctt ttcagacggc atcgcatatt tgaagaccgt
    19741 atccgaacag tttgatgttg tctttctcga cccgccgttt gcatggcagg actggcaaat
    19801 cctgttcgat gccttgaagc cgtgcctgaa cccccgggca ttcgtctatc tcgaggcggg
    19861 tacgctgccg aatattcccg attggctgac ggaatataga gaagggaaat cggggcagag
    19921 tacatttgaa ttaagggttt tccaagtggc tgaataatat gcgctttgat gatcatttcc
    19981 gagttgtaaa cattcgtttg caaccgtccg gttcaaaaaa accttgtgct ataatccgcg
    20041 cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa tgtcgctttt tattaccgac
    20101 gagtgcatca actgcgacgt atgcgaaccc gaatgcccca atgatgccat ctcgcaaggc
    20161 gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt gcgtcggaca ctacgatgaa
    20221 ccgcagtgcc agcaggtttg cccggtggac tgcatcctga ttgacgaaga acatcccgaa
    20281 acccatgacg agttgatggc gaaatacgaa aagattatcc agtttaaata aattcttttt
    20341 aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac ggaaaagcaa
    20401 gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa gggggcagac
    20461 tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc aaaattctta
    20521 cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag ccttccaagc
    20581 ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat gtagctcagg
    20641 ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca tgggcaccat
    20701 ctctcgatta ttcatttctt taaggcttag atatatagga tattg
//
LOCUS       DAWKQJ010000017        20709 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000017 DAWKQJ010000000
VERSION     DAWKQJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.017"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..239)
                     /locus_tag="WJL20_000490"
     CDS             complement(63..239)
                     /locus_tag="WJL20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682678.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WJL20_000491"
     CDS             complement(242..1600)
                     /locus_tag="WJL20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ7682679.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMVAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALINNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1724..3369)
                     /locus_tag="WJL20_000492"
                     /pseudo
     CDS             complement(1724..3369)
                     /locus_tag="WJL20_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3392..4870)
                     /locus_tag="WJL20_000493"
     CDS             complement(3392..4870)
                     /locus_tag="WJL20_000493"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ7682680.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANDYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4895..6640)
                     /locus_tag="WJL20_000494"
     CDS             complement(4895..6640)
                     /locus_tag="WJL20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ7682681.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="WJL20_000495"
     CDS             complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="WJL20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ7682682.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="WJL20_000496"
     CDS             complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="WJL20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ7682683.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQAALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="WJL20_000497"
     CDS             complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="WJL20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ7682684.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8678..9895
                     /locus_tag="WJL20_000498"
     CDS             8678..9895
                     /locus_tag="WJL20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7682685.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLGRGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            10027..10103
                     /locus_tag="WJL20_000499"
     tRNA            10027..10103
                     /locus_tag="WJL20_000499"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10061..10063,aa:Met,seq:cat)
     gene            10111..10186
                     /locus_tag="WJL20_000500"
     tRNA            10111..10186
                     /locus_tag="WJL20_000500"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10144..10146,aa:Ala,seq:ggc)
     gene            complement(10477..11298)
                     /locus_tag="WJL20_000501"
     CDS             complement(10477..11298)
                     /locus_tag="WJL20_000501"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ7682686.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11331..11975)
                     /locus_tag="WJL20_000502"
     CDS             complement(11331..11975)
                     /locus_tag="WJL20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7682687.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11978..12841)
                     /gene="ftsN"
                     /locus_tag="WJL20_000503"
     CDS             complement(11978..12841)
                     /gene="ftsN"
                     /locus_tag="WJL20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7682688.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQTGQNAFKSPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12952..14448)
                     /locus_tag="WJL20_000504"
     CDS             complement(12952..14448)
                     /locus_tag="WJL20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ7682689.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSANVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVLKAIGFRRAL"
     gene            complement(14462..14782)
                     /locus_tag="WJL20_000505"
     CDS             complement(14462..14782)
                     /locus_tag="WJL20_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ7682690.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15265..16791
                     /gene="putP"
                     /locus_tag="WJL20_000506"
     CDS             15265..16791
                     /gene="putP"
                     /locus_tag="WJL20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ7682691.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17042..20647
                     /gene="putA"
                     /locus_tag="WJL20_000507"
     CDS             17042..20647
                     /gene="putA"
                     /locus_tag="WJL20_000507"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ7682692.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4836 a         5995 c         5067 g         4811 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcgccc agttcgccca tatcgcccat tacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc cgggttcgca ttataagtat
      601 cgtcaatcag ggtcgcgccc ttgatgccgg ctttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtca aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgatca aagcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatgc ccatttcgat gacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttgcc gcccgaaccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccac catttgcaac gcggtaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt ttttatcgcc tgttttactg tggtaaaaca
     1681 cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt ttagacgtat
     1741 ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga
     1801 taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa aaatcatagc
     1861 cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat
     1921 taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac gaaattttaa
     1981 ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc
     2041 ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag ctttctttat
     2101 tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa
     2161 catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta tccaaacgtg
     2221 tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg
     2281 catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc aaccccgcgc
     2341 ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg gcataggtgg
     2401 aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc gtccgaaaac
     2461 ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc cccaaagtct
     2521 gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc aacagccctt
     2581 tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc atataatccg
     2641 aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca acatgacgga
     2701 tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg gcggcatatt
     2761 gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac aggccggcat
     2821 caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag gacaaaatca
     2881 aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt acgtcagcaa
     2941 ataccccccc gatacacaat atctccaaac tgtcaagcca aaaatgaaaa ataatgcctg
     3001 cacaaaataa agcgacccgg gaagattgcc gacaaattca cgcgcctcgg caggattgct
     3061 ttccaatatc gaaccgacta tctgataaga cggcgcacca tacagccagc cgaccggcaa
     3121 atatagggcg gttgtgccga cgtaaatcag taaaacaact gaagaaacgc gcgggaaacc
     3181 gcgtgccaat aataataaaa atacaaccga agccaaagca cccacggcag aataacggta
     3241 gccgtattca tattccaagt gataacctga tgcgatggcc gcgccaaaac aaaaggctaa
     3301 aagcgcgcaa atgcccaatg tattttttaa tgtctgatcc atatttttta ttcgggcgga
     3361 ttgtttcata cgatgccgtc cgaatatccc attatttacg agttaacaaa gcctgtccga
     3421 cgatttcaag atcggaaaaa cggtgcttca cgccttgtac atcctgatag ttttcatgcc
     3481 ctttgccggc aatcaggatg atgtcgtttg cggcggcttg ttcaaccgca taacggatgg
     3541 cggcggcacg gtcggcttcg acgcattcgg gcgcgggaac ggcaggcagg atgtcgttga
     3601 tgatgtcgtg cggattttcc aaacgcgggt tgtcgctggt gacgacgact ttatccgcac
     3661 cctgtacggc tgccgcgccc atcagcaggc gtttgccgcg atcgcggttg ccgccgcaac
     3721 cgaatacgca ccacaaagcc gcaccctgcg gtttgatttc ctgcaaggtg gcgagtgctt
     3781 tttccaatgc gtcgggcgtg tgggcgtaat cgacgacgac caagggcttg ccgctgttca
     3841 tgatgcagtc catgcgccct gaagcgggac ggatttttgc cagcacatcc aataccttat
     3901 ccaacggata gtcgttggcg cacagcaagg cgatgcaggc ggcgaggttt tgcgcgttga
     3961 accgtccgag caggcgtgtg cggcatttcc cttcacccca cggggttcgg aatacggctt
     4021 ctatgccgtc tgaagaggcg gtaaagtcgg taatgcggat gtcggcgtgt tcgctgaagc
     4081 cgtagctata aacggccaaa tcggggcagt cttttttcag acggcctatg agttctgcgc
     4141 cgtatccgtc atccacgttg atgactgcgt gtttcaagcc atgccagtaa aacaggcgcg
     4201 acttgatggc gccataggct tccatcgtgc cgtggtagtc gaggtggtct cgggtgagat
     4261 tggtaaagat tgcgctgcgg aatggcacgc cgttgacgcg cgactggtca agaccgtggc
     4321 tggagacttc catcgcggcg gctgttgcgc cttgttgacg gaaacggtag agcagggttt
     4381 ggacatcgac gggggcgggt gtggtatgcg tggtttcttc caatgcaccc caaaagccgt
     4441 tgccgaccgt gccgacaatg gcggtttttt cgcccaacaa atcggcagct tgcgccagcc
     4501 attgcgtgat ggaggttttg ccgttggttc cggttacgcc ccagaatttg agaccgtctg
     4561 aaacgttgcc gtaaacttgc gccgccaata tgccggcacg gtgtttcaaa tctttgatgc
     4621 cttgattggg gactttccat tcgggattcc acgcaaattt gccgtcgtcg tcccaaaaaa
     4681 caaaagccgc gccgttggca acggcggcgg ggatataact gcggccgtcg gcatattcgc
     4741 ccggacaggc aacgaaaata tcgccttgtt tgatttggcg gctgtctgaa tgcaacaaac
     4801 gccctgctgc gtttgcacac gacagggtcg ggatgccggt ttcagccaaa ggggttaact
     4861 tgctgaacat aaaacaatct cgttgatact cggattaaga cggtgttttg acggctgcgg
     4921 cggtcagtgg cttggtcggg gaaatgccca agatgttcag gctgccgccc ataatttttt
     4981 tgaagggcgg ccctgccact acgccgccgt aataaccgtg ggcagtcggt tcgtcaatgg
     5041 ttaccgccac aatcacacgg gggtttttgg cgggggcaaa accgataaag gtagcgatgt
     5101 gtttgttgtc ggcataacgc ccgttgacga acttgcgcgc cgtgccggtt ttcgcgccga
     5161 catcgaaacc gtccaccgca cccgccgtac cggtgccgcc cggctcggtt acggaaacca
     5221 tcagattgcg tacctcgcgc gcggtcgatt ctttgaatat gcgtttgcct tgcggcgcaa
     5281 ccgcctgttt ttcaaagctg accggcagca aaacgccgtc gtgcgtcagt gcggtatagg
     5341 cgcgcgccaa ttgcagcagg ctcaattgca ggccgtaacc gaaagacatc gtcgcctgtt
     5401 cgataggccg ccacctgcgc caatttctca acaaacctgc agtttcgccc ggaaagcccg
     5461 agtgcatacg cacaccgatg cccaactcat gatagaagtc atacatttct tcggcaccga
     5521 aacgcgcaga cagtttgctt gtgccgacgt tggacgattt ctgcatgatg ccgcgcacat
     5581 ccaaagaggg gtaaacatga gtatcgcgca cgggagacgg tccgatttta taaggctgcg
     5641 tattcagccg ttcgttcaaa tcggttttgc ccgcatccaa tgccttcgca atcacaaacg
     5701 gtttgattgc cgaaccgggt tcgatcatat cggttacggc acggttgcgc cgctgttcgc
     5761 tgtctgcccg gccgggcctg ttgggatcgt aggcgggcgt attggccaag gcgaggattt
     5821 cccccgtgcg ggcatccaaa accaccaccg ttccggcttt tgcctgatgg tattcgaccg
     5881 ccttgttcaa ctcttcatag gccaaggtct gaatcctctg atcgagggaa aggatgatgt
     5941 ctttgccgtt tttcggggct ttattgcgcg gggagtccaa gctgtccaca atattgccct
     6001 gccggtcccg caaaacgact tccgcgccgt cttcgccatg caggctgtct tcaagcgaaa
     6061 gttccaaacc ttcctgacct ttgccgtcaa tatcggtaaa tccgatgacg tgtgcaaaca
     6121 ggttgcccat cgggtaatgg cgttttaatt ctttttcaaa tacaaagttt tccaaaccca
     6181 aggctttgac ctcttcggca accttgggat cgagctgccg cttaatccag ataaacgact
     6241 tgcctttctg ttcgagcttg ttcctcaaaa catcaaccgg cacatcgaca agctcggaca
     6301 ggcgttccaa ttgtgcggca gacggcattt ccttcatctc tttaggcacg gcaaacaggg
     6361 actccgtcgg cgcgctcaac gccaaaaccg caccgttccg gtccgaaacc gtaccgcgtg
     6421 tagccggcaa tgtttgagtc cgcacaatcc ggttgtcgcc ctgttctttc aaaaagttat
     6481 acgttaccgt ctgcagatac agtccgcgag caatcagacc ggcaaacaag accgctattg
     6541 ccatcaggac gaagctgatc cgtccgttac tggtcatcgg ctttttgacc tgctcttctt
     6601 tgggcagcat ccgaggctta tattcgctct taatcaacat ttttacttct cgttattatt
     6661 atcctgacgc aggaatccga ttccggcaca caggctgctt ctatctttga tgcgccacca
     6721 taaaggtatt gcccgaaacc ggcggatgga ggttttgttt ttctgccgcc gccctgatcg
     6781 cttcgtggtt cgccaaacgc gcctgttgca gcctcatttg cgcataatcc tgctccaagg
     6841 cgatttcctg ttttttcgcc ttgtccaaag ctgtgaagtt gagcctgtac tggttttgct
     6901 gcatcacaac ggaaaaagcg gaaacgcaca ccgcaagcag cagaaggaaa ttcaatttgt
     6961 tcattgccat tcagacgttt ttctctgtga ttgttccggt atcgaaccgg cagtccgctc
     7021 cgccacacgc aaaaccgcac ttctcgccct cggattggcg gcaatttccg cctcacccgg
     7081 ctttaatgcc ctgcccacga ttttcagggg cagctcgggc aaatccgctt ccctgaccgc
     7141 cgcccaacgc ggcagaggcg cgtgttgcga atattttttg acaaactgct tcacaatgcg
     7201 gtcttccaac gaatggaaag caatgaccgc caaacgtccg ccctctttca gacgacacat
     7261 gacctgcggc aatactgccc ctacttcttc aagctcgcgg ttaataaaga tgcggaccgc
     7321 ctggaaggtg cgcgtcgcag gatcctgccc ccgctcgcga gtacggacgt tttgtgccac
     7381 gatctgcgcc agcttgcggg ttgtatcgat tggactttcc gcccgttgcg caacaatggc
     7441 gcgcgcaatc tggcggctaa accgctcttc accataattc ttgattacct cgtgcaaatc
     7501 ctgttccgac gcaaccgcta tccactctgc ggcagacata ccgcgcgtcg tatccatacg
     7561 catatccaaa ggggcatcga aacgaaagct gaaaccgcgg ctgccgtcat cgatttgcgg
     7621 agacgaaatc cccaaatcaa acagcgcacc gtccaccttg ccgataccca aaccgtccaa
     7681 tgccgcctga aacgaagcaa aaccgccatg cacgacaccg acccgcttgt ccgaacgcgc
     7741 cagctcttct gccacagcaa tcgcctgagg gtctttgtcg aaaacaatca accgccccgc
     7801 atcgcccaaa cgcgacaaaa tcagccggga atgccctccc ctgccgaacg taccgtccac
     7861 atagacaccg tcttcgcgca cggcaagcgc atccaccgcc tcattcagca agaccgtgat
     7921 atgccggtaa ctttctgctc cactcacaat tgcaaatccg tctgactcaa ctggaaggca
     7981 agttcgtcag gatcgtcatc caaagcctga accatctcag cctcccactg ctcgcgaccc
     8041 caaagctcca aacggttggc acgaccgacc aaaacgactt cacggtcgaa atccaccctc
     8101 ttcctcagtc cggcaggaac cagcacccgg ccggcgctgt cccattccaa aatttccgcg
     8161 ttatgcagca aaagattttg aaaccgccgc aaaacagggt tatccgccac ttttaagttt
     8221 aaaagttgcg ccgcaacctt ttcccactcc gcaacagggt acatcaacag cttgtgtttc
     8281 gactcgagcg ttaccactac ggcaggcgta tagaggcgcg acagaatgtc acggaatttg
     8341 gcaggaacag ccaaccgccc cttactgtcg atgcttaatt cgtgtgcgcc gccgaacatg
     8401 acatgtccca agccgaaatc aaaatcacaa gggtaaaaga gacactttgc cccacaattc
     8461 cccaccaatc gacactataa gaaattttaa acactcggtc aaatcagggc atgaaaactc
     8521 attaacatat ctgaaatttt tattcctttt aaaaacaata agataaaaaa tgacgacaac
     8581 ggcacggcgg gtgcggtaca gaataatcga accaataaac aactatatat gattaattta
     8641 ataatataaa cacaatatat agtattaaga taaagccatg acagcacccg taccaacgtg
     8701 taatatgtcg ggaaatccaa taaatttaca caagctaaca cttatcatgc ccctccccct
     8761 cccctctccc gaagcacggc aattctcgct caaactgcaa accctcattg ccgaagaaat
     8821 cggcaaacac ggcaactgga ttccattttc acgttttatg gaattggttt tatacgctcc
     8881 gcaatacggc tactacaccg gcggcagcca taaaatcggc aataccgggg attttattac
     8941 cgcaccgacc ctcacctctc tgtttgcaca gacactggca cgccaacttc aagaacttct
     9001 atcccaaacg gcgggcaata tctatgaatt cggcgcggga accggacagc ttgccgccga
     9061 tttgttgggc agcatttcgg acggcatcag ccgttactat attattgaaa tatcgccgga
     9121 gctggcagca cgtcagaaaa acctgattca agcacgcgca ccggaagcat ctcaaaaagt
     9181 tgtccacttg accgcacttc ccgaagcgtt tgacggcatc atcatcggca acgaagtact
     9241 cgatgccatg cctgtcgaaa tcgtccgtaa aaatgaaggc ggctcattcg agcatgtcgg
     9301 cgtttgccta gataatgacc gttttaccta ttcggcacga ccgctgcacg acttacagct
     9361 atctgccttg gcttccctct attttcctca aacagattat ccctacacca gcgaactaca
     9421 tccgcaacaa tatgccttta tccgcaccct tgcctcaaga cttggacgcg ggggtatgat
     9481 attcatcgac tacggttttg atgcagcgca gtattaccac cctcaacgca atcaaggtac
     9541 tctgatcgga cactaccgac atcacgttat ccacaatcct tttgacttca tcgggttggc
     9601 cgacctgacc gcgcatgtca actttaccga cattgcccaa gccgggacgg atgccggatt
     9661 agatttgata ggttaccttc cccaatccca tttcttattg aacctgggca ttaccgagct
     9721 attggcacag acggggaaaa cggattcggc agcctatatc cgtgaagctg ctgccgttca
     9781 gaaactgatt gaccagcatg aaatgggcga actgtttaaa gtcatcgcat tcggaaaaaa
     9841 tatcggcatc gactgggcag gattccgctt cggcgacatc tgccacaaac tctaaccctc
     9901 atgccgcctg aatccgcttc agacgacata aactttttaa catttaaaaa cagtcaacta
     9961 attcaaaatt aaaaaatacg gcttgtcaaa aaaacagaaa aacatataat agtgtcttca
    10021 cgaaacggcg aattagctca gtcggttaga gcagaggaat cataatcctt gtgtccgggg
    10081 ttcgagtccc tgattcgcca ccaaattttc gggggtatag ctcagttggt agagcgcttg
    10141 catggcatgc aagaggtcag cggttcgatc ccgcttacct ccaccagata aaaaagcaca
    10201 gaccgtaaaa aggtatgtgc ttttttatta cctgattacc ggcaaataaa gaataaacca
    10261 ctaccttcaa aacaggcaat cgactttaaa cctatcgccc cgcctgtcct gattttatag
    10321 tgaattaaat ttaaaccggt acagcgttgg ctcgccttgc cgtactatct gtactgtctg
    10381 cggcttcgtc gccttgtcct gatttttgtt aattcactat atcagcccgc cagacaaacc
    10441 cgacccgaat aatgtcttca ggtcgggttt atggtttcat tcccaactta tccagcccga
    10501 caaccacaat attatgatga cgatgccgaa aacaatgcgg taataggcaa acgggatata
    10561 gtttttcttg gaaacaaact tcagcagtgc tttgaccgct accaaaccgg aaacaaaagc
    10621 ggcaataaag cctatcagaa tcaaaccgac atcatgcaga gtgaaaaacc ggtaatgctt
    10681 caggacatca taagccgttg cggcaaccat catcggcaca gccaagaaaa acgagaattc
    10741 tgtcgcagtt ttccgttcga tgccccaaag catcccgccc ataatcgtac tgcccgaacg
    10801 ggacgtaccc ggaaccagtg caaacacttg ggcaacgccg atcatcaagg catcaatcgg
    10861 acgcaatgca tcaacatcgg caattttagg ctctgctcgg ctttggcgtt tctccaccca
    10921 caaaataaaa aaaccgccca aaaccagcat gactgcaaca ctcaaggggt taaacagata
    10981 ctctttgatt tgtttgccga acaacagccc catcacggcg gcaggtataa aagcaatggc
    11041 aagattaagg acgaaacggt tggctttccg gtcttttccc acgccatgca acacattgct
    11101 gaaacgttgc cggtattcaa acactaccgc caaaactgca ccgagctgga tggcaatttc
    11161 aaaaaccttg tgattgctgt gaaaaccaat cagattgccg aacacaatca aatgcccggt
    11221 gctggaaatc ggtaaaaatt cggtaaaacc ttctaccaag cccatcatca gggctttcag
    11281 gacaatcaga aaatccattg cttgcgcttc tttcggatac gggagttcgg ctatttctgt
    11341 acagcagggg tctgacgctt gcgttcttcc ctgactttgg caaccaattc tttaatcgta
    11401 tgaacgccgc cgtcaaagcc gttattgaag ataacgcggt attttccgcc gacaataacg
    11461 gtcggcgtgc tgtcaatacc gtattgttcc gtcagtttct gcatttttaa tgcggcggcg
    11521 gcagcttcgg gagaatcata ggcgcgcatc agttttttgc cgtcaaagcc tttttgagac
    11581 aaagcccact ttccggcaac cgacctgttt tccaagcgga ttttttgttc gtaaactgct
    11641 ttaaacacag cagggtttgc ctgatatttc aaacccgaca aattgacggc ggccgccatc
    11701 ctggccagac cgagcatttc aggctgccag accacgtgct ccgtcctcaa ataggcatca
    11761 gacggcaatg ccttgcccaa tttcaataac aaaggatcga aatgatggca atgtacgcag
    11821 aaatagccga aaaattccaa aacctcaatt ttgcccgact gttcttgagg aatgggttta
    11881 tccaacacaa gatagtcttc cctttccgtc agggcatatg cctgcgcgga caacactgcc
    11941 gacagcagca gcggcaacag atgtttgagc ttcataatta tttgctttcg atagaacgga
    12001 tcaggctggc gacttcatgt tttttcaact cgtcctgcat ttttttcacc gcatcggcag
    12061 acatattgcc gctttgcacc cggtaaagcg ttttatgtcc cgcctgataa ccgaccacct
    12121 tggaagatat gcccaagatt gccagtttgg cacgctgtcc ttccgcgctc cggcggtcgg
    12181 catacgcgcc catttgcaga taatgcgttg cttccgcctt gtcgggcgtt ttcattttct
    12241 gcacttcttt ggcagcggca ctgcgcgctt tttcgatgct gccgctgttg aggatttgtt
    12301 ccggggtcgg tttgggtgca actttttcct tctccgcctt tttctcttct tttgaagctt
    12361 ttttctctgt ttctttagat ggttttaccg cttgtttttt aaccgtttcg gcatctttct
    12421 tctgcgcttt ttccccgacg gtttgttcac gctcttccgt cagtgctttc ttgcgcactg
    12481 cctgtccgtc cgacttttcc cgctccggct cgtccgcctt ttcctcaacc tcgtcggctt
    12541 tgtcggcaac gggctgcttg tcggcagctt tttccgcatc cgactgctct gcctctttcg
    12601 cagcatccgg ttcggacaag gcgttttgat cggccggttc aggttggatg tcttccttag
    12661 gctggttttt cggtttcagg atttccgttt ctgcaggctg cttcgacgaa gccgggcttt
    12721 taaacgcatt ttgacctgtt tgattcagat aaaacaaaat accggcaata atgactgttg
    12781 ccagtatcag accgaagaag aaaccggaca gaccttttcc ggattgggaa aatttgttca
    12841 taaacatacc ttaatgtatt tcagacggca ttaacgccgt ttgcgtgcgg acggatattc
    12901 taacaatatt gccatatttg ggcaaaaccc gctccgatgc cttttataaa gtcataaagc
    12961 gcggcggaaa cctatggctt tcaaaacatg gcttctgccg acttcttcgt cgcctgccaa
    13021 atccgccagc gtacgcgcca cgcgcataat gcggtggaaa ctgcgggcgg agagggagag
    13081 tttttccaac agtccgccca aggcttcctg cgcttctttt tggatgcggg cggacgtgtc
    13141 gagttcgctg acgctcaagg cggcattcac tttgccctgc cgtgcgtatt gtttgtcacg
    13201 ggcggcgatg acgcgttcca aaacgtttgc actgctttcc cctgcttcct gctgcatcag
    13261 ttcggcggcg gacaggctgg ggacttcgat ggtcaaatcg atgcggtcaa gcagcgggcc
    13321 ggaaattttg ctgcggtaac gcgcgacact ttcgggcgtg cagcggcagg gtttgacggg
    13381 atgcccgaga taaccgcacg ggcaggggtt catggcggcg acaagctgga atttggcggg
    13441 atagacggct tggcgcgccg cgcgggaaat gtggatttca ccgttttcca aaggttcgcg
    13501 caacacttcc aaaactttgc ggtcgaactc gggcaattcg tccaaaaaca aaacgccgtg
    13561 atgtgccaat gaaatctcgc ccggacgcgg atccgaaccg ccgccgacca tagccgccgc
    13621 gctggcgctg tgatgcggac tgcggaaagg acggttgctg tcgagttgtt gttggtggtt
    13681 gggcaggagc gaacgcaatg cccacacttc gaccaattcg tcttcggtca gcggcggcag
    13741 gatgccgggc agccgttggg agagcataga cttgcccgtt cccggcggac ccatcatcaa
    13801 aaggctgtgt ccgcctgcgg cagcgatttc caaagcaagg cgggcagtgt gctgaccttt
    13861 cacatcacac aaatcaggtt gtccgccatg ttcagacggc atttgcggta cggaacattc
    13921 ggtttgcgcc aaaggttcga taccgttcaa atgggcggcg acttcgccca aagagcgcgc
    13981 gccgtaaacg gtaatgccgc gcatcacggc ggcttgtcct gcgttttctt caggcaaaac
    14041 aaatgcacgt tttgcctgca taccctgcca cgccattgcc aacgcgccgc gcacggggcg
    14101 caacagcccc gacagtgcca attcccccgc aaactcgtat tccgccagct tttcgggcgc
    14161 aacctgcccc gatgcggcaa ggatgccgat tgcaatcggc aaatcgaaac gccccgactc
    14221 tttgggcagg tcggcggggg cgaggttgac ggtgattttt ttggcgggga attcaaaacc
    14281 gctttgaata atggcggcac ggacgcggtc gcgactttct ttgacttccg tatcgggcag
    14341 tccgacgatg ttgaaatgtg gcaggccgtt ggcaaggtgg gcttccactt cgaccaacgg
    14401 cgcattcata ccgctcaagg cgcggctgta aaccaaggca agcgacatat ttcagacggc
    14461 cttattcgcc ggcttcggtt tgctgcctga tttcggcgac ggcttcttcg gcagcggctt
    14521 cagccgcttc caatgctgcc cgttcgggat tttgcgcggc ttcgagtttt gccaaacgcg
    14581 cttccaaagc cgccagtttg gtacgagttt tgattaaaac ctgctgctgg atgtcgaatt
    14641 cttcgcgcgt aaccagatcc atacggttga acgcgccgcc cagcatcgcc ttaatatttt
    14701 tttccacatc tttggcaggg ctgttggcga tggtttcgct gattttcgaa ccgacttctt
    14761 caaaaagctg cttgccgaac ataatctgta tccttcctga acatatcaaa ttcaatcggc
    14821 tattgtataa ggaaaaatgc cgtctgaaaa cgggcggcgg ataatcggca aaacataccg
    14881 cgcctccttt gcggttgcta aattacacct caaaacacct gccgcctgaa aacggatttc
    14941 atattccgcg caacgccaga ttacaagaca ctacaaaaca atatactgtt ttaaatgatt
    15001 tttccgacgc gcatcgtttc aaactcggct ttttaagcca ttaagtgctt tgcaaacaac
    15061 agaaattggg ttatgctgaa acggattatt tacaatttca tatagtttta ttacatatct
    15121 tattgtgatt gaagataatt tatccgaatt cccctttcgg gtatccggat tttccgttgt
    15181 acttttatta gaaaaacatt tcaggcgcaa gttgcttgca atttcaaagc ctgtaattct
    15241 gaagttttag acgaggaata gcacatgaat cccatgtaca ttacttttgc aatctatctg
    15301 gttgcagtcc ttctcatcgg gcttgccgcc tatttttcta cgcgcaattt cgacgattac
    15361 attttgggcg ggcgcagcct gggcccgttt gttaccgcga tgtcggcagg cgcgtccgat
    15421 atgtccggct ggctgctgat gggtctgccg ggcgcgattt acctgagcgg tttgaatgag
    15481 gcttggattg ccatcggcct cttggtcggc gcgtatttca actggctctt ggtggcgggc
    15541 cgcctgcgcg tgcataccga atacgccaac aatgcgctga cgctgccgga ttatttcttc
    15601 caccgcttcg gcgcgggcgg acacttgatg aaagtggttt ccgcactgat tatcctgttc
    15661 ttcttcacga tttattgcgc ctcgggcatt gtggcgggcg caaccctgtt ccaaagcctg
    15721 tttgaaggta tgacttacaa tcaggcaatg tggctgggcg cgggcgcgac catcgcctat
    15781 accttcttgg gcggtttctt ggcggtcagc tggacggata cgctgcaggc ttctttgatg
    15841 attttcgccc tgattttaac gccggtgatg gtctatctgg gcttgggcgg cgcggaacag
    15901 atgtctgccg cgattcaaag cgtcgccgca ggcacgggca aagaatacgg cagcctgttt
    15961 gccggtacga ccgtcatcgg catcatttcc accgccgcat ggggcttggg ctatttcgga
    16021 cagccgcaca ttttggcgcg ctttatggcg gccgaaagcg cgaaatcgct ggtatccgca
    16081 cgccgcatcg gcatgacttg gatggcgttg tgccttgcgg gcgcggtggc ggtcggttat
    16141 ttcggcattg cgtatttcgg tgcaaatccc gacaaagttt cttctatgag cggcaaccac
    16201 gaacgcatct tcatcgcgct ttccaccctg ctgttcaacc cttggatcgc cggcatcatt
    16261 ttgagcgcga ttttggcggc ggtgatgtcc accctgtcct gccagctttt ggtttgctcc
    16321 agcgcgatta ccgaagactt ctacaaaggc tttttgcgta aaaacgcgca acagtcggaa
    16381 ctggtatggg tcggccgcct gatggtcttg gcgattgccg tgatttccat cctgattgct
    16441 tccgatccga acagcaaagt attgggactg gtgtcttacg cgtgggcagg tttcggtgcg
    16501 gcattcggcc cgattgtgat tctgtccgta ttgtggaaac gcatcactgc ctacggcgcg
    16561 ttgtcgggta tggttgcagg tgccttaacc gtggtcgtat gggcggaatg ggtgaaaaaa
    16621 ccggctcaag cggcaggcga aagcggcttg ttgacgatgt acgaaatcgt cccgggcttt
    16681 attgtttgct tgatcgttgc cgtgttggtt tccctgttca acaaagaacc ttcgcgcgaa
    16741 acccaagaac gctttgaaaa agccgacgca gattaccgcg ccgcccgata aacccctttc
    16801 agacggcacg gcatcccggt tcgtgccgtc tgaacccgtt tccgggtcaa acacaaataa
    16861 accttgcctg catacaggcc ttatttgtgt tttacccgtt tcattttcaa agaaacggaa
    16921 gcacctaccc tgcccgcatt tgaagccttg tatttcatct gcggatttat tacgatatta
    16981 ccgtattcag accgcaccga tgccgtctga accccccgaa aaactttgga gaatccaaaa
    17041 aatgtttcat tttgcatttc cggcacaaac tgccctgcgc caagcgataa ccgatgccta
    17101 ccgccgtaat gaaatcgaag ccgtacagga tatgttgcaa cgtgcacaga tgagcgacga
    17161 agagcgcaac gccgcctccg agcttgcccg ccgtttggtt acccaagtcc gcgccggccg
    17221 caccaaagcc ggcggcgtgg atgcgctgat gcacgagttt tcactctcca gcgaagaagg
    17281 catcgcgctg atgtgtctgg cagaagccct gctgcgtatc cccgacaacg ccacgcgcga
    17341 ccgcctgatt gccgacaaga tttcagacgg caactggaaa agccatttga acaacagccc
    17401 ttccctcttc gtcaacgccg ccgcctgggg cctgctgatt accggcaaac tgaccgccac
    17461 aaacgacaga caaatgagtt ccgcactcag ccgcctgatc agcaaaggcg gcgcaccgct
    17521 catccgccaa ggcgtaaatt acgccatgcg cctgttgggc aaacagttcg taaccggaca
    17581 gaccattgaa gaagccctgc aaaacggcaa agaacgcgaa aaaatgggct accgcttctc
    17641 cttcgatatg ttgggcgaag ccgcctacac ccaagccgat gccgaccgct actaccgcga
    17701 ctatgtcgaa gccatccacg ccatcggcaa agatgcggca ggacaaggcg tttacgaagg
    17761 caacggcatt tccgtcaaac tctccgccat acatccgcgc tactcgcgcg cccaacacgg
    17821 ccgtgtgatg ggcgaactgt tgccgcgcct gaaagagctg ttccttttgg gtaaaaaata
    17881 cgatatcggt atcaacatcg atgccgaaga agccaaccgt ctggagctgt ctttggattt
    17941 gatggaggca ctcgtttccg atcccgattt ggcaggctac aaaggcatcg gtttcgttgt
    18001 ccaagcctac caaaaacgtt gtccgttcgt tatcgactac ctgatcgacc ttgcccgccg
    18061 caacaaccaa aaactaatga tccgcctcgt caaaggcgcg tattgggaca gcgaaatcaa
    18121 atgggcgcaa gtggacggct tgaacggcta tccgacctac acccgcaaag tccacaccga
    18181 catctcctac ctcgcctgcg cgcgcaaact gctttccgcg caagacgcgg tatttccgca
    18241 attcgccacc cacaacgcct acaccttggg cgcaatctac caaatgggca aaggcaaaga
    18301 ctttgaacac caatgtctgc acggcatggg cgaaaccctg tacgaccaag tcgtcggccc
    18361 gcaaaactta ggccgccgcg tgcgcgtgta cgccccggtc ggcacacacg aaaccctgct
    18421 cgcctacttg gtgcgccgcc tgttggaaaa cggcgcgaac tcgtctttcg tcaaccaaat
    18481 cgtcgatgaa aacatcagca tcgacacgct catccgcagc ccgttcgaca ccatcgccga
    18541 acaaggcatc cacctgcaca acgccctgcc gctgccgcgc gatttgtacg gcaaatgccg
    18601 tctgaactcg caaggcgtgg acttgagcaa cgaaaacgta ttgcagcagc ttcaagaaca
    18661 gatgaacaaa gccgccgcgc aagacttcca cgccgcatcc atcgtcaacg gcaaagcccg
    18721 cgatgtcggc gaagcgcaac cgattaaaaa ccctgccgac cacgacgaca tcgtcggcac
    18781 agtcagcttt gccgatgccg cgcttgccca agaagcggtt ggcgcagccg ttgccgcgtt
    18841 ccccgaatgg agtgcgacac ctgccgccga acgcgccgcc tgcctgcgcc gtttcgccga
    18901 cctgctcgaa cagcacaccc ccgcgctgat gatgcttgcc gtgcgcgaag caggcaaaac
    18961 gctgaacaac gccattgccg aagtgcgcga agccgtcgat ttctgccgct actacgcaaa
    19021 cgaagccgaa cataccctgc ctcaagacgc aaaagccgtc ggcgcgattg tcgccatcag
    19081 cccgtggaac ttcccgctcg ccatctttac cggcgaagtc gtttccgcat tggcggcagg
    19141 caacaccgtc atcgccaaac ccgccgaaca aaccagcctg attgccggtt atgccgtttc
    19201 cctcatgcac gaagccggca tcccgacttc cgccctgcaa ctcgtcctcg gcgcaggcga
    19261 cgtgggtgcg gcattgacca acgatgcccg catcggcggc gtgattttca ccggctcgac
    19321 cgaagtggcg cgcctgatca acaaagccct tgccaaacgc ggcgacaatc ccgtcctgat
    19381 tgccgaaacc ggcggacaaa acgccatgat tgtcgattcc accgcacttg ccgaacaagt
    19441 ctgcgccgac gtattgaact ccgccttcga cagcgcggga caacgctgct ccgccctgcg
    19501 tatcttgtgc gtccaagaag acgttgccga ccgtatgctc gacatgatca aaggcgcgat
    19561 ggacgaactc gtcgtcggca aaccgattca gctcactacc gatgtcggcc ccgtcatcga
    19621 tgccgaagca cagcaaaacc tgttgaacca catcaacaaa atgaaaggcg ttgccaagtc
    19681 ttaccacgaa gtcaaaaccg ctgccgatgt cgattccgaa aaatccacgt tcgtccgccc
    19741 catcctgttt gaattgaaca acctcaacga actgcaacgc gaagtcttcg gtcccgtcct
    19801 gcacgtcgtc cgctaccgcg ccgacaaact cgacagcgtc atcgaccaaa tcaacagcaa
    19861 aggctacgcc ctgacccacg gcgtacacag ccgcatcgaa ggcacagtac gccacatccg
    19921 cagccgcatc gaagccggca acgtttacgt caaccgcaac atcgtcggcg cagtcgtcgg
    19981 cgtacagccc ttcggcggac acggtctgtc cggcacaggc cccaaagcag gcggttcgtt
    20041 ctacctgcaa aaactgaccc gcatccccga atgggttgcc ccgaccctga gccaaatcgg
    20101 acaggcagac gaagccgcgc tcaaacgcct cgaagcactg gttcacaaac tgccgttcaa
    20161 cgccgaagag aaaaaagccg cagcggccgc tttgggacac gcccgcatcc gcaccctgcg
    20221 ccgtgccgaa accgtcctta ccggcccgac cggcgagcgc aacagcatct catggcacgc
    20281 gccaaaacgc gtctgggtac acggcggcag cctcctgcaa gccttctgcg ccctaaccga
    20341 acttgccgcc tccggcgtac aggcagtggt cgaacccgac agcccgctgg cttcctacac
    20401 cgccgacttg gaaggtctgc tgctggtcaa cggcaaaccc gaaaccgccg acatcagcca
    20461 cgttgccgcc ctgtcgcctt tggacagcgc gcgcaaacag gaacttgccg cccacgacgg
    20521 cgcactcatc cgcatcctcc cttcggaaaa cggactcgac atcctgcaag tgtttgaaga
    20581 aatctcttgc agcgtcaaca ccacagccgc cggcggcaac gccagcctga tggcggttgc
    20641 cgactgattt taccgaaatc cccgagcgcg gcccgtgaac caatgccgtc tgaaaacctt
    20701 tcagacggc
//
LOCUS       DAWKQJ010000018        20487 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000018 DAWKQJ010000000
VERSION     DAWKQJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.018"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WJL20_000508"
     CDS             complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WJL20_000508"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ7682693.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPSKVNPVVQTQLIVDHSLAVECGGYDLDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVCQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2826..3614)
                     /locus_tag="WJL20_000509"
     CDS             complement(2826..3614)
                     /locus_tag="WJL20_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ7682694.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPILLIFVALYFVFSPKLDGSKEGKARISFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIISMF"
     gene            complement(3620..4273)
                     /locus_tag="WJL20_000510"
     CDS             complement(3620..4273)
                     /locus_tag="WJL20_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7682695.1"
                     /translation="MRLVGNFKGGGATKLENEDVSKVDKSSHSGIALGAHTEAPYHAA
                     TKIFNNHSPAPSSLVLTARYNPLSEPTTVIPLQPILEKLHYLDLLALTTENFDFTRSE
                     TFTSGKGTGGSKVSILEFNNDGTIGVKYNSYRFTTNVHAPQSVKDALNRFNELINNSE
                     QYKINLQPNTCMIINNTLALHCRDVIQDNRRLLIRIFGYKNNIEYISLEDDPVLAQG"
     gene            complement(4490..4672)
                     /locus_tag="WJL20_000511"
     CDS             complement(4490..4672)
                     /locus_tag="WJL20_000511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682696.1"
                     /translation="MDKFQSIINLTADAALFVQNKATEIATIGGCQAKGVREIASNAG
                     KTLLEKYLNSEQKDEI"
     gene            complement(4805..5959)
                     /gene="prpC"
                     /locus_tag="WJL20_000512"
     CDS             complement(4805..5959)
                     /gene="prpC"
                     /locus_tag="WJL20_000512"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ7682697.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPEREGHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     EEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(6045..6920)
                     /gene="prpB"
                     /locus_tag="WJL20_000513"
     CDS             complement(6045..6920)
                     /gene="prpB"
                     /locus_tag="WJL20_000513"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ7682698.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(7546..8856)
                     /locus_tag="WJL20_000514"
     CDS             complement(7546..8856)
                     /locus_tag="WJL20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ7682699.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8995..10173)
                     /gene="ftsZ"
                     /locus_tag="WJL20_000515"
     CDS             complement(8995..10173)
                     /gene="ftsZ"
                     /locus_tag="WJL20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ7682700.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(10292..11536)
                     /gene="ftsA"
                     /locus_tag="WJL20_000516"
     CDS             complement(10292..11536)
                     /gene="ftsA"
                     /locus_tag="WJL20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ7682701.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(11622..12350)
                     /locus_tag="WJL20_000517"
     CDS             complement(11622..12350)
                     /locus_tag="WJL20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ7682702.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(12340..13254)
                     /locus_tag="WJL20_000518"
     CDS             complement(12340..13254)
                     /locus_tag="WJL20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ7682703.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(13367..14776)
                     /locus_tag="WJL20_000519"
     CDS             complement(13367..14776)
                     /locus_tag="WJL20_000519"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ7682704.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14931..15998)
                     /gene="murG"
                     /locus_tag="WJL20_000520"
     CDS             complement(14931..15998)
                     /gene="murG"
                     /locus_tag="WJL20_000520"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ7682705.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(16002..17327)
                     /locus_tag="WJL20_000521"
     CDS             complement(16002..17327)
                     /locus_tag="WJL20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ7682706.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(17453..18790)
                     /locus_tag="WJL20_000522"
     CDS             complement(17453..18790)
                     /locus_tag="WJL20_000522"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ7682707.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18875..19255)
                     /locus_tag="WJL20_000523"
     CDS             complement(18875..19255)
                     /locus_tag="WJL20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7682708.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGDADAQ
                     NNLGAMYAEGQGVRQDDTEAVRWFRQAADQGLAQAQFNLGAMYYKGHGVRQDRALAQE
                     WLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(19382..20464)
                     /locus_tag="WJL20_000524"
     CDS             complement(19382..20464)
                     /locus_tag="WJL20_000524"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ7682709.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4947 a         5587 c         5082 g         4871 t
ORIGIN      
        1 aaggtttcct caattccact ttctggattc ccgcctgcgc gggaatgacg atttacagta
       61 gatgcctgcc atatcgagaa ttacgtgaat gagcaaaatg ttgaacccga cccacgctac
      121 gtcttgcttt tcagacgacc tctaagccgc gttcccttcc aaaaaatcct gtgcaaaccg
      181 ttgcaacacg ccgccggctt catataccaa tacttcttct gcagtatcga ggcggcaggt
      241 aacgggaact tcgacggttt ctccgttttt acggtgaatc acgagggtca ggtcgcagcg
      301 cggtgtacgt tcgccgataa cgtcgtaggt ttccgtgccg tccagttgca gggtgtggcg
      361 gttggtgccg ggtttgaatt gcagcggcaa gacgcccatg ccgatgagat tggtgcggtg
      421 gatgcgctcg aaaccttcgg cggcgatggc ttccactccc gcgagccgca cgcccttcgc
      481 cgcccagtcg cggcttgagc cttgaccata gtccgcaccg gcaatgatga tgagcggctg
      541 tttgcggttc atataggttt cgatggcttc ccacatgcgc atggtttcgc cttcgggttc
      601 gacgcgggcg agcgagcctt ggcacacgct gccgtcttcg tttttcacca tttcgttaaa
      661 cagtttcgga ttggcgaagg tagcgcgttg ggcggtcaag tggtcgccgc ggtgggtggc
      721 gtaagagttg aagtcttctt caggcaaacc cattttcgcc aaatactcgc ctgcggcact
      781 gctggccaaa attgcattgg acggtgagag gtggtcggtg gtgatgttgt cgggcaaaat
      841 cgccagcgga cgcatacctc ttaatgtgcg ttcccctgcc agtgcgcctt cccagtaagg
      901 cggacggcgg atgtaggtgg acatcggacg ccaatcgtac agcggactgg gtgctttttg
      961 cgctgtgccg gtgtcgaaca tcggtacata cacatcgcgg aactgctgcg gtttcacata
     1021 ttcggcaacg acggcatcga tttcttcatc ggcaggccaa atgtctttca ggcggatttc
     1081 cttgccgtct gcaacgccga gtacgtcgtt ttcaatatcg aaacggatac tgcctgccag
     1141 cgcgtaggca acgaccaacg gaggcgaagc gaggaaagcc tgtttcgcat acgggtggat
     1201 acggccgtcg aagttgcggt tgcctgataa tacggcggtg gcgtacaaat cgcggtcgat
     1261 gatttctttc tggattttcg gatccagcgc gccactcatg ccgttgcagg tggtgcaggc
     1321 gaaggcgacg ataccgaagc cgagtttttc catttcgggc aacaggcccg cttctttcaa
     1381 atagatttcg gctacttttg aacccggggc aaacgaagat ttcacccaag gtttgcgttt
     1441 caagccgaga cggttggcat tgcgtgccaa gagcgcggcg gcaacaacgt tgcgcgggtt
     1501 ggaagtgttg gtgcaactgg taatcgcggc gatgatgacc gagccgtcgg gcatttggcc
     1561 gtccgaaggc tcttcgtaag gcttcgccag ccctttcgcc gccaaatcgg cggtcgcaaa
     1621 acgggcatgc gggttacttg ggcctgccat attgcgcgtt acgctgctca aatcaaattt
     1681 caaaacgcgc ggataaacgg cggttttcaa ggcatctgcc cacaagcctg cggttttggc
     1741 gtaggtttcc accaatttca cctgcgcgtc gtcgcggccg gtcagtttca aataatcaat
     1801 ggtttgctca tcaatagcga acatggcggc agtcgcaccg aactccggcg tcatgttgga
     1861 aatggtcgcg cggtcgccga tggacaggct tctcgcgccc tcgccgaaaa attcgacaaa
     1921 cgccccgacc acgcgctctt tgcgcagaaa ctcagtcagc gccaacacaa tatccgtcgc
     1981 cgtaatgccc gcctgccgtt tgccggtcag ctccacaccg acaatatcgg gcaggcgcat
     2041 catggacgcg cgacccagca ttacggtttc cgcttccaaa ccgcccacgc ccacggaaat
     2101 cacgcccagc gcatcgacgt gcggcgtgtg cgaatccgtg ccgacgcagg tatcggggaa
     2161 agccacgccg tttttgactt ggacgacggg cgacattttt tctagattga tttggtgcat
     2221 gatgccgttg cccgccggaa tcacgtccac attttcaaaa gcggtttttg tccagttgat
     2281 gaagtggaaa cggtcttcgt tacgtctgtc ttcgatttcg cggtttttgc ggaacgcatc
     2341 gaggtcgtag ccgccgcatt ccaccgccaa agagtggtca acgataagct gggtttgcac
     2401 caccggattc actttggaag gatcgccgcc tttttcggca atcgcatcgc gcaagcccgc
     2461 caaatccacc agcgcggtct gccccagaat atcgtggcac accacccgcg cgggatacca
     2521 cggaaagtcg atttcctgtt ttccctcaat caactggccc agccagcttt gcagcgtcgg
     2581 caaatcgact ttgtccgcgc ggttgaccaa attctccgcc aaaatgcggc tcgtgtaagg
     2641 cagcttgtcg taagagccgg gcttgatgtc ctcacacgcc gcacgcgcgt cgtagtattc
     2701 caaatccgta ccgggcagcg gtttgcggta acgttggttg gcagccatgt cggttctcct
     2761 ggggatctgt ttttcttgtg gtttgaggtt tcagccgacg ttttgaaggg gtcgtctgaa
     2821 agggtttaaa acatcgaaat aatcatctga tacagcggat ttctctcgtc tatcaacaat
     2881 ttcacagcca tcgaaatgct gatgacaatc agcagcggct taatcagctt cgaaccgaag
     2941 cggacggcaa atctcgcacc taaattcgca ccgacaaacg caccgaccgc catcgttgcc
     3001 gcaatcggga aaataatcga accgtgcagc aggaataccg atagcgaacc aagattgcag
     3061 gcaacgttcg ccaatttggt gtaagacatc gcgttcaaca gcttgcagcc gagcaaaaca
     3121 ataaaggcaa tcagaaaaaa cgagccgaca cccggtccga acacaccgtc gtaaaaaccc
     3181 aaaagcggtg caaccgtcag cccgaataga aaaaaagata ttctggcttt gccttcctta
     3241 ctgccgtcga gcttgggcga aaacacaaaa tacagcgcga caaatatcaa caaaatcggc
     3301 acgaccgcca gcagaatatc tttggaaacc aagctgaccg ataatgcacc gaccacgccg
     3361 cctgcaaacg atgctgccgc aatcgggaga cctttcttcc aatcaatcaa acctttgcgt
     3421 gcaaaagaaa ccgtagccga aaacgtagca gcggctgctt gcagcttgtt ggtggcaatt
     3481 gccgacacgg gaggaatacc tgccaacaag agtgcaggca gcgtaatcaa accaccccca
     3541 cccgcaatcg catcgataaa tccggcaatc atcgcaacca aacccaaagc gagtattata
     3601 tataaatctt ccatgtttct tatccttgag ccaatacagg atcatcttct aaagaaatat
     3661 actcaatatt atttttatat ccaaaaatac gaattaacaa tcttctgtta tcttgaataa
     3721 catcacggca atgcaaagcc aacgtgttgt taatgatcat acaagtgttt ggttgtaagt
     3781 tgattttata ttgttcagag ttgttgatta attcattaaa tctattcaaa gcatctttaa
     3841 ccgattgggg tgcatgcaca ttagtagtaa atctataaga gttatattta acaccaatag
     3901 ttccatcgtt attaaattca agtatagata ctttacttcc ccctgttcct tttcctgacg
     3961 taaacgtctc actacgagta aagtcaaaat tttctgtcgt taatgccaat aaatccaaat
     4021 aatgcaactt ttccaaaata ggttgtaatg gaattactgt tgtcggttct gatagcggat
     4081 tgtatcttgc agttaaaaca agagatgatg gtgcggggga gtgattatta aaaatctttg
     4141 ttgccgcatg gtaaggagct tcagtatgcg cccctaatgc aattcctgaa tgtgaacttt
     4201 tatctacttt gctaacatct tcattttcaa gttttgttgc tccacctccc ttgaaattac
     4261 caacaagtct cattattttc ccatgattat caatattata agcaaatgca aattgctcta
     4321 ctgttgccaa taaaatctga ttagatgcag ccaatttcaa acattctaag ttttcttcaa
     4381 gctcttccat ttttggtaaa gaacttggaa tgctaattat tgaattatca atgtttttat
     4441 cataaagatt attaaaaata actacacaag ttttttgatg aatgaatttc tagatttcat
     4501 ccttttgttc tgagtttaaa tatttttcca ataaggtttt ccctgcatta cttgcaattt
     4561 ctcttactcc ctttgcttga cagccaccta ttgttgcgat ttccgttgct ttattttgaa
     4621 caaacaaagc agcatcagca gttaaattaa taattgattg aaacttatcc atttaataca
     4681 tacccttttt attaggttaa atttttaaac ttatttcaga caacctttcc aataaggcaa
     4741 ggtcgtctga aatcctcagc tttgcatacc gaaataaaga acaaactgct attgcattct
     4801 tcaattatcg ttcttcaatc tccacaaacg ccaaatcttc tggacctgtg tagtttgcgc
     4861 tcggacggat gattttgccg tctttgcgtt gctcgagaac gtgtgcgctc caaccggttg
     4921 tacgggaaat tacgaacagc ggtgtgaaca tagcggtcgg tacgcccaat ttttggtagg
     4981 aaacggcaga gaaccagtcc agattcggga acattttttt ctcttcccac atcacgcttt
     5041 ccaaacgttc ggcaatgtca aagaggcgca tatcgccggt ttctttgctc aaaccgcgtg
     5101 ccacttcttt aatgacaacg ttgcgagggt cggaaatggt gtacaccgga tgaccgaaac
     5161 cgatcacgat ttctttgcgg ccgatgcgtt cgcggatgtc ggcttcggct tcgtcggcat
     5221 tgcggtagcg tttttgaata tcgtaagcca cttcgttcgc gccgccgtgt ttcggacctt
     5281 tcaacgcgcc gattgctccg gtaatgctgg agtacatatc agagcctgta ccggcgatca
     5341 cgcgggcggt aaaggtagaa gcgttgaact cgtgttcggc atacagaatc agtgaaacgt
     5401 gcatggcttt gatgtgtgat tcgcttgggc gtttgccgtg caacaggtgc aggaaatgac
     5461 cgccgatggt ctcttcttcg ctttcaactt caatgcgttt gccgttgtgc gaatattgat
     5521 accagtacaa gaggatactg ccgaggctgg cgatcagttt gtcggcaatg tcgcgcgctt
     5581 cgctttccgg atggccttca cgttcaggat gaacacagcc cagcatggat acgccggtac
     5641 gcatcacgtc catcggatgg gtatgtgcag gcaggctttc caaaacttta atcacacgga
     5701 taggcaggcc gcgcatggat ttgagcttgg ctttataagc ggccagctcg aatttgttgg
     5761 gtaaatggcc gtgaatcagc aggtgggcaa cttcttcaaa ctcacatttt tgtgccaaat
     5821 ccagaatgtc gtaaccgcga tagctcaaat cgttgccggt acggccaacg gtacacaaag
     5881 cggtattacc ggccgcaacg ccagaaagcg caacggattt tttaggtttg agggtcgggg
     5941 tttgagtagt ttcagtcatg gtatttctcc tttgtgtttt tatgggtttc gggttttcag
     6001 acgaccgatg cggatttgtt gaaaggcagt ctgaaagcgg taaatcattt ttgaaacaat
     6061 ttatccagtt tttgctcgaa ggcatgatag ttcagatgct cgtacagctc ggcacgggtt
     6121 tgcatactgt ccaccaccgc cgcctgagtg ccatcgcgca taatcgcttc gtaaacattc
     6181 agagcggctt tgcttgctgc acggaacgat gacagcggat acagcaccag cgacacgccg
     6241 ttttcagcca gctcgctttg ggtataaagc ggagtggaac caaactcggt aatgttcgcc
     6301 aacacgggca ctttcaccgc atctgcaaat tggcggtaca tgttcaaatc ggtcatggct
     6361 tcagggaaaa tcatgtccgc accggcttcg acacaagctt gggcgcgttc gatagcggca
     6421 tccaaacctt ctaccgccag cgcatcggta cgcgccataa tcacgaagtt ctcatcaacg
     6481 cgcgcatcta cggcagcttt gatacggtcg accatttcat ctttagatac aatggctttg
     6541 ttcggacggt ggccgcagcg tttttgcgct acctgatctt cgatgtgaac cgctgcaaca
     6601 ccggcgcgtt caaagttgcg aatggtacgg gcaatattga atgcaccgcc ccaacccaca
     6661 tcgatgtcca ccagcagagg cgtatccacg ttgtccgtaa tgcgtcgcgc gtcaatcagc
     6721 acatcttcca ttgtggtaat gcccaaatca gggataccgc aagaacaggc tgccacgccg
     6781 ccgccggaca gatagatggc tttgaaaccg ctttgggtgg ccaatcgtgc aaagtaagca
     6841 ttgacgcaac cgacgacggc aagcggattc gattctttca cggcttggcg gaaacgtgct
     6901 ccggcagagt gttgactcat catatttctc ctttatagac ttttttcagt atggacaggc
     6961 ttccatcaca ttcggacggc aaaacacatc catccgacgc gtcgggcatc ccaatccaat
     7021 cattaaaaat atatgggaac aattatctta ttgatattta aaacgaatca aagaaaaaca
     7081 gcagaccgtt cggaattatg cggcaaaacc gcagacaaga agaaaacaag gggattattc
     7141 agaaaagggg aaaacatctg aattggtttc atagtaatgt tcctttgtag tattgtgtag
     7201 ttttattttt cgctaatcta aaacacaaga ctgaaaatgt caaatatttt taaggagact
     7261 cattacataa ttttaatatt ttattttaat tccatgatta aactagatta attttgaatt
     7321 atttgtttcc gataaaatta acaggaaatt gagaaaaatt ttcgataatc aaaatcctga
     7381 aaacttataa aatattttat acataaaaac aatgtattat ttttaagcaa tccgatgtat
     7441 tttattatgt atttgatttt tatataattt aataaaaatt aaatataccg gcaaacaaaa
     7501 tgccgtctga aaacctttca gacggcattt ttaatatcga atcaatcagc ccaaatacca
     7561 gaatttcagt gcccacaata cggcaacaat ccataccata ggcggaacgt ctttggtgcg
     7621 gcggcataaa agtttaacca cggcatagct gatgaagccg aaggcgatgc cgtctgcaat
     7681 cgaataggta aacggcatga agacaatggt caggaatgcg ggtgcggctt ccgtcatatc
     7741 gtcccaatca atatccctcg cactgcggag catctgcgtg ccgacataaa gcagggcggg
     7801 cgcggtggca aaagcgggaa cacttttcgc caaaggtgaa aacatcaggc aggcgagcat
     7861 caatacgccg acggtaaccg ccgtcaggcc ggtccgtccg cctgccgata cgcccgccgc
     7921 gctttccaca taaggcgtgg tggaagaagt acccaaagcc gcacctgcca caatggcggt
     7981 agagtctgca agcagtgctc gtttcaggcg gggcagctta ccgtccacca gcagcccggc
     8041 acggtgggat atgccgacca gcgttccggt actgtcgaac agatcgacca agaagaagac
     8101 gaaaatcaca ctgaccatgc tgacggtaaa caggccttca aaatccatct gcataaaagt
     8161 cggcgcaatg ctcggtactt cgccgatgat gccgtgaaat tcattcaaac ccatcaggct
     8221 ggcaatgacg gtaatcgtca aaatggtgat gatgattgcg ccttgaacgc ggaaatgtcc
     8281 caatacgacc accatagcaa aaccgaacag tgccaacaac gcggacggct gatgaatatc
     8341 gcccaaaccg accaaggttg ccggattggc aacgataatg cctgcgcctt tcagggaaat
     8401 cagtgccaaa aacaaaccga taccggcagc aatcgacatt ttcaaaccca taggcagtgc
     8461 gttgaccagc atttccctga ctttaaaaaa gctgaacagg atgaaaatca gaccggagat
     8521 gaacaccgca cccaacgcaa cctgccaagg cacgcccata cccttaacga cggcaaaggt
     8581 gaaataggca ttcagcccca tccccggtgc gagtgcaatc ggatagttgc cgacaaaacc
     8641 cataacaaaa cagccgatgg cagacgcgat acaggtagcg acgaataccg cccccatatc
     8701 catgccggtc tcgcccaaaa tcagagggtt gacgataacg atgtagcaca tcgtcaaaaa
     8761 agttgtcaaa cccgccatca actcggtacg caccgtcgta ccgtttgcct tcagcttaaa
     8821 aatcccgtcc aacagtgttt gttttgaagt gtccatatct gaaagtctgt atcaagtgga
     8881 aaaatctgaa tccgccgacc gcaaaccgta cgaaattgca acatcttgaa aaacatacct
     8941 cccaaaccgg acaaaccgat tcgggaggca gcaggtttac gggcaaacag cacattattt
     9001 gtctgaattg tgttgacgac gcaaaatcgc agggatttca aagtcgtcaa gtacggactg
     9061 attgtcgaaa tccgcagcgg taaggttcat cgtgcggata ccgcgattgg tgcggatcat
     9121 accttcgaca ttgtggcttt gctcctgttt ggacggggca accgcttcta cctcccttgc
     9181 cggaacaaaa tcgaccgcgc ctttttcttt cagaccggta gcgataatgg taatccggat
     9241 ggcatcttcg ctcatggtct cgtcttcagc agcaccgaat ttgcattcca aatcgggatg
     9301 cgcgctttgg ttgacgattt tcatgacttc ggacaactcg gacattttca agcaacccgg
     9361 agcagtcgta atattgacca gcacaccgcg cgctccgtcc aaggttacat cgtccagcag
     9421 cggactggaa atggcctggt cggtcgccat acgcgcacgg tcgatacctt gggcataacc
     9481 cgaacccatc atagcgatac cgcggttgct catcacggtt ttcacgtcgg caaagtcgag
     9541 gttgatgatt tcgctcgggc aagttaccac ttcggaaatg cctgcgaccg catcgcgcaa
     9601 tacattgtcg gcggcacgga aggcttcgcg catcgttacg tcttcaccca atgcagtcat
     9661 cagtttgtcg ttcgggatga taatcagcga atcgacgtgt tctttcaact gttccaaccc
     9721 tgcctgtgcg acatggacgc gcttaccttc atatgcgaac ggtcgggtaa ccacggcaac
     9781 ggtcagaatg cccaaagact tggcaatctc agcaacaacc ggcgcggaac cggtaccggt
     9841 accgccgccc ataccggtcg tgataaacag catattcgca ccgcgaatgg cttcttcaat
     9901 ggcttcccgg tcttcctggg ctgccgcacg gccgatatcg ggattcgcgc ccgcgcccaa
     9961 accgcgtgtc agattcgtac ccaactggat tctcttcgcc gcatggtttt ttgccagaga
    10021 ctgcgcatcc gtattggcac tgataaactc cacaccgcgc acattgttgg caaccatgtt
    10081 attgattgca ttgcaaccgc cgccgcccaa gccgattact ttaatcaccg cagggctgac
    10141 tgccgattct gccacgtcgt aaacaaattc cattcaaaaa ctcctgctcg ccccattcag
    10201 aggacggttt aaataaatta ttattcatta tataagaagt atcttgctgc tggcaaaata
    10261 cttctcacct gtcaaacggc aatccacctg ttcagaagct gttttcaatc caccgtttca
    10321 atcttgccaa caaaccgccg cccccttccc tctcttgcac tgcaccgttt tccggctgcg
    10381 gcaagtttcc ttccagcttg catgctgcat gaagcagccc gatggcggta gaaaagcgcg
    10441 gtgtgcggac gcggtcggac aaaccgccca tttcttgggg cgcaccggtg cgtacaggca
    10501 aatcgaagat tttttcggca aattccacaa tcccggtcat catggacaca ccgccggtca
    10561 gaacgatacc cgcattcagc acttctttgg ggaaacccga tttttgcagc tcgcccagca
    10621 ctacgccaaa aatctcctga atacgtgcac tgatgattgc tgccaaaacc ttactggaaa
    10681 cctgacgcga tgtccggtca cccacgcccg gaacttcaat catctcaccc aagccttccg
    10741 tatcgcatga tgtcacgcca taatggattt taatgtactc ggcggcatcg agaggtgttc
    10801 tcaacgattt ggacaaatct ttggtaatca gattaccacc ggccggaatg acggacgtat
    10861 ggcggatggc accgttcata taaacggcaa tatcggtcgt tccgccacca atgtcgatga
    10921 cgcatacgcc gaggtctttt tcatcttcag tcagcaccgc ctgcccgctt gccaacggct
    10981 gaagcatgat ttgatcgctt ttcaagccgc accgctcgat acatttttgg acattctgca
    11041 ctgccgtact tgcaccggta atgatgtgca cccgcgtatc cagacgcaca ccgctcatac
    11101 cgatgggctc cctcacgcca agttgggtgt cgataatgta gtcttgaacc acggcatcga
    11161 gaattttttg atcgggcggg atattgattg cctttgccgt ttcaatggcg cggtcgatgt
    11221 ctgcctgcgt gacttccccg tctttaattt taaccacacc ttgcgaattg agactgcgga
    11281 tgtggttgcc tgcgatacct gtggtaacgt gggtaatttt ggtatccgcc atcagctcgg
    11341 catcattgac cgcctgcctg atggcttgga cggtggcatc gatattggtt accatgcccg
    11401 cgcgcaagcc ccgtgaagga gcctgcccca aaccgacgat gttgattttg tcgtcatctt
    11461 gaacttcccc gatcagtgcg aggactttag acgtaccgat atccagtacg ctgatgtatc
    11521 tttgctgctg ttccattgtt cgtctgctct taaaactgat tgaaatttgc gtcgcaccgt
    11581 ttcagacggc acggtcgtaa tctgtccgat acctgttccc actattcttc ggattctttt
    11641 tcgggtaaac cgtcggaagc atagcggact gaaaatccgt ccttatacct catatccaca
    11701 taggataacc gatttttatt tttacgcaac agatgctgcc acgcttcggt aaaaagccgg
    11761 aggcgtttca tctcgttttc ccgtccgagc ctgacggtga tgccgttgtc caaaacgaca
    11821 atccacgccg aacgtgccgt ataggtcatc tctttgatgc ccaaaccctg ttttgccaaa
    11881 acagtcgaaa attcgtcata acggcggagc atttcggcag acgttccttc cgcgcctctg
    11941 aataccggca ttccgggtct gtccaagcgg gcttcaaaaa cattgccttc gccgtccacc
    12001 aaggcatggt cgccccaacg cgcgaccggc ttgcgctcgg tcaggacgac ctcaaccgtg
    12061 tcgggaaaac ggcggcgcac catgaccgac gcaatccacg gataccggcg gtaggcctcc
    12121 tgtgcgccat tgatgtccgt cctcaaaata ttcccatgga tgtattcttt cgccaaactg
    12181 cccaatgcct tcttatcgga ataaaccagg ttgcccttca gcgacacctg cttgacgggc
    12241 agatgattcg aattgtaaaa ccaaaccagc ccggacgcag caagcagcat cgccatcatg
    12301 acaagcagcc agcgcgtcag ccgttccatc gcttcggcat tatcccacat gtgcggtctt
    12361 caaaatttca atacataaat cggcaaaacc cacgcccgta acggcagcgg atttcggtac
    12421 taaactatgg ctcgtcatac cgggcagggt gttgatttcc aacagataga gtttgccgtc
    12481 ggtatctttg aggaaatcga cgcgcacgca gccttccgca ccgattgcct gcgcgccgcg
    12541 aaccgccagt tcgcgcatca ggctttcttc ggcttcggtc aaatcttccg aaggacattg
    12601 ataaatggtg tcgtctcggt tgtacttggc ttcgtagtcg taaaactcgg ttgcgggaat
    12661 gatgtgtatg ccgggcagcc ctttgccgtt caggacgggg caggaatatt cgccgccgcc
    12721 gataaaacgt tcggcaatga tttcgccctg aaggtgtttc aattcttcgt aaacgctttt
    12781 cagacggcct tttcctttga cttttaccac gcctacgctg ctgccttcgg ctgccggttt
    12841 cacaaacatc ggcaggccca atttttcttc gacggcatcg aaatcagtgt cgtcgtgcag
    12901 gacggcgaac tcgggaacgg gcaatcccaa tgcctgccaa atcagtttgc agcggtattt
    12961 gtccatgccg atggcggatg cggcgacacc gctgccggta tagggaatgc ccaacagttc
    13021 caatgcaccc tgaaccgccc cgtcttcgcc gtaagtaccg tgaaggatgt tgaatgccgt
    13081 ctgaaaacct tgtgccttca attcagacaa tggggtttct ttaggatcga aggcgtatgc
    13141 gtctatgcct ttgcttttta aagcattcaa aatggcggtg ccgctgtcca gcgagatttc
    13201 tcgttcgctg gaaaaaccgc ccatcaatac ggccactttg ccaaaattct gcattgtttt
    13261 tgttctttcc tgattgcttt atgcttgttg ccagaggtcg tctgaaacct gatttgcggt
    13321 ttcagacgac ctttatatga tgttccgtct gtcaggaggg tgtgcctcaa atctgtttcg
    13381 acaattccag tagcgcggcg gggacgcggt tgatgcttcc cgcgcccata ttcaacacga
    13441 tgtcgccgtc ctgcaaaacg ttcaacagca tttcgggcag atcggcaacg ttttcgcagt
    13501 aaatcggctc gagtttgccc aacacgcgga tggcgcgggc aagagcgcgg gaatcggcgg
    13561 cggcaatcgg ctcttcaccg gcggcataaa cttcggtcag caccagcgcg tcaacggtat
    13621 tgaggacttt ggtaaagtct tcaaacaaat cgcgcgtgcg ggtatagcgg tgcggctgga
    13681 aggcgagtac caaacgtttt tccagatacg cgccgcgtgc ggcggcaagg gtcgccgcca
    13741 tttcgacggg gtggtgtccg tagtcgtcca ccaagagcgc ggtcccgccg tttggcaact
    13801 tgatgtcgcc gtatttttgg aagcggcggc cgacgccttc aaagccgagc aagccttttt
    13861 ggatcgcttc aaccgatgcg ccgacttcca gcgccacgcc gatggctgcc aatgcgttca
    13921 gcacgttgtg tctgccgggc atattcagca cgacttcaaa cgacccctgc tcatgtcctt
    13981 tcatttgaac atggacggtg aatttcattt gcgcgccgac gttttcgatg tcggtggcgt
    14041 agatgtcggc ggtatcgtcc aaaccgtaag tagcataagg tttgctcact ttgggcaaaa
    14101 tcgcgcggac gtgttcgctg tcaatacaca aaaaggcttt gccgtagaag ggcatacggt
    14161 ggatgaaatc gataaacgcc tgatgcagtt tttcgacgct gtgcccgtag gtatccatat
    14221 ggtcttcgtc gatattggta acgacggaca taatcggtgt caggtgcaga aaggatgcat
    14281 ccgactcgtc ggcttcggca acgatgtatt cgcctttgcc caagcgggcg ttggtgcctg
    14341 cggcgttgag tttgccgccg ataacgaaag tcgggtcaag tcctgccgcg ccgaggatgg
    14401 aggcggtcag gctggtggtc gtggttttgc cgtgcgtgcc ggcaatggcg atgccgtcac
    14461 ggaagcgcat caactccgcc aacatcaggg cgcgcggaat aacgggaatt tgctgctcca
    14521 acgcagcgac aacttcggga ttttcttttt tgacggcggt agaggtaacg acgacatccg
    14581 caccgttaac gtgttctgcg gtatggccgg gataaacttg aatgcccagg ctgcccaaat
    14641 gctcggtagc ggcattacgc gcctgatccg aaccggaaac tttaaaaccc aaattgtgca
    14701 agacttcggc gatgccgctc atgccgacgc cgccgatacc gacaaaatgg atgttggtca
    14761 ctcgattttt catcataatg ttgcgttccg gtggattttc gatgcgtaaa ggcgttattt
    14821 taaagggctg accgtttgcg cgccatagtt ttctgacaaa tatatagcgg attgaaataa
    14881 aaaacatcca tgctgtctgg acggcatttc agacggcatg gttcggcagt ttacgccgca
    14941 cacgcaatcg cggcttcggc aacgtcgtcc gcgctgtgcg gcaatgccaa agtacgggcg
    15001 ttttctgccc atttgagaca tttttcgcgg ttcaggctgc cgaggatttc ggcgagtttt
    15061 tccgccgtca actgggtttg cggcaacagc aatcccgcct ccgcctgcac cataaaacgc
    15121 gcgttggcgg tttggtgatc gtcaaccgcg tgggggtacg gaacgagcaa cgcacccaat
    15181 cccgccgccg tcaactcggc aatcgtcagc gcgccggcac ggcaaatcac caaatcggca
    15241 tcgcggtagg cggacaccat gtcggtaata aattccacgc attcggcttt cacgcccagc
    15301 gcgtcgtaat ccgcctgcaa gctgcccagc ttgccccgtc ccgattggtg gtacatctgc
    15361 ggacgcgcat tgtcgggcag caaagccaat gcctgcggta cggttttgtt caaaacgtcc
    15421 gcgcccaaac tgccgccgac caccaaaatt ttcagacggc cttcacgccc ttggaagcgt
    15481 tcggcaggca cgggcaggtt gctaatatcg gcgcggacgg ggttgccgac caagccgcct
    15541 tcgtggctga acgctttcgg aaaagcgtac aacacccgct tcgcccagcg cgacaggtgg
    15601 cggttggaca aacctgccac ggcgttttgc tcgtgaatca caatcggcac gcccaagagt
    15661 ttcgccgcca aaccgccggg gaaggtaacg aagccgccga agccgatgac gcactcgaca
    15721 cggtgtttgc ggataatccg ctgcgcttcg cggacggttt gatacaaagt aaacggcagc
    15781 atcagcttgc gtttgatgcc gttgccgcgc acgcctttaa tcgccagcgt ttccaagcgt
    15841 atgccgtatt gcggcacgat acgctcttcc atcgaatcct tgctgcccag ccaaatcaca
    15901 tgatggccgc gcgcgcgcaa tgaatccgcc accgccagcg cggggaaaat atgtccgccc
    15961 gttccgcccg ccatcagcat aaaggtttta ccgcccatga tttactccac ccgataaccg
    16021 cgcattttcc ggcggttttc ataatctata cgcaacagca gcatcatgct gatcagcatg
    16081 aaaaagactg acgaaccgcc ataggacatc aacggcagcg tcagaccttt ggtcggcaaa
    16141 gcaccgatgt tcacaccgat attgaagaaa ctttggatac cgatccaaat gccgataccc
    16201 gaagcgatat aggcgttgaa agtcaaaccc aaatcgcgcg actgcttgcc gatggaaaac
    16261 gcccgcacca ccagccagcc gtaacagaat atcagcacgc acataccgaa gaaaccgaat
    16321 tcttcggcga tgatggcaaa aataaaatcg gtatgcgctt ccggcagaaa gccgcgtttg
    16381 ctcaaactcg cacccaaacc cataccgaac cactctccgc gcccgattgc catcagagag
    16441 tgggtaagct ggtagccggc accctgcggg tctttccacg ggtccaaaaa tgccactacc
    16501 cgctgcacac ggtagggagc ggcggtaatc atcagcacca tcccgcccaa gacgctgcct
    16561 accaggacga aaaaatattt ccacggcaat cctgccaaaa acagcattcc aacggcaatg
    16621 acggtaatga cgacaaacga accgaaatcc ggctgtacca ttatcagcac caaaccgaac
    16681 gccaccagca taatcggcag gatgatcgcc cggaaacggc cgtacatttc taatgtttca
    16741 cgacgtgcct gcggattggt ggcggacatg atcagattgg ccgtcccccg ccaaatcgac
    16801 tgccaaccca aactttccat gctgcgcaac acttcttcac ggcgcgtgaa caggcttgcc
    16861 aaataaagga tgaccgccag cttgaacagc tcggtcggct ggaaattcaa cggacccaaa
    16921 ggtatccaac gggtcgcgcc attgatttcg cgcccggcaa tcaatacgac taccagcaac
    16981 aggccggata gggcaaaaat ccacggcaca agccgccgcc atgtcctcat cctgcaaaga
    17041 aaccataaca aaccgctcgc tatcaagccg gcaacgacga accccgcctg tctggtcaaa
    17101 tagaaaaact gatcgccgcc ttcttttgat gccaaataca cagaagccga ataaatcatc
    17161 agcaggctga acgccgtcat cagcaccacc atccacaaaa gcggcgcgtc gaatttcctg
    17221 ccgtcgcgca caatcggcct gtcgagcagc agagtgtgga caccgtcgcc cacttttacc
    17281 aatacttccg aaatcttcaa aaaaaccacc tgccagtctg tttgcacctg ccgcaaaggg
    17341 caaaaatttc agacggcaga caatgccgtc cgaacatacg atacatccca aatcggtatt
    17401 ctaaatcttt acttgccgcc caacaatgac ggcgtttgca tttcagacgg catcacaaag
    17461 ccttaaacgc ttcgataaac acttccgaac ggtgcgcgta gcctttaaac atatcaaaac
    17521 tcgcacaagc cgggctgagc agcacgatat cgcacgcttc ggcttgggca tatgccgtct
    17581 gaacggcttc ttccaaagtg gcgcagtcgg tcatattcag accgcagccg tccaaatcgc
    17641 ggcggatttg cggcgcatca acgccgatca ggaacacgcc ttttgccttg ccggcaagcg
    17701 cgtcgcgcag aggcgtgaag tcctgccctt tgcccatgcc gcctaaaatc acgaagagcg
    17761 gattttgcaa accggcaatc gcggcggcgg tcgcaccgac gttcgtgcct ttgctgtcgt
    17821 cgatgaacac cacgccattt ttctcgccga ttttttccac gcggtgcggc aggccttgaa
    17881 aggttttgac gtgttcgagc aatgcttcgc gcggcaaacc gacggcttcg cacaaggcaa
    17941 cggcagccat cacgttagtg gcgttgtgca agccttgcag cggaatatct tgcgtggcaa
    18001 tcaaatcttc attgccttgt ttcaggcgac ctgtctcacg ttccaaccaa aaatcggctt
    18061 cgtattccaa cgaaaaccat ttcacctcgc gcccggcgcg cttcatcgca cgacagaacg
    18121 catcgtccgc attcaaaacc tgcacgccat cgccacggaa aatcttggct ttggtatgcg
    18181 cgtagtcgag caagtcgtcg tagcggtcga gatggtcttc ggaaatgttc agcaccgtcg
    18241 ccgcagtcgg gcgcaggctt tcggtgtttt ccagttggaa gctggaaagc tccaacaccc
    18301 acacgtccgc ctttttgcct tcgcgctgcc attccgcctc caaaaccggc gtgccgatat
    18361 tgcccgcgat aacggtatcc agcccgcact tgatacagag atagccgacc aggctcgtta
    18421 ccgtggtttt gccgttgctg ccggtaatcg caattacctt gtcgccccgg cggttcacaa
    18481 tgtccgccag caattcgatg tcgcccaaca cgcgtccgcc gttttgcttg aacgcctcga
    18541 tatccggctg ccgctcgctg atgccgggac tgagcgccag aatatcgaaa ccgttgtcca
    18601 gcgcatcttt cagacggccc gtgtaaaaca ccagcccgtc aaacatctta ccgatttgcg
    18661 acacgcgttc cggcttcagc tccgcatcat acgcagcaac ctccgcgccg tttttgcgca
    18721 ggtaggcaat catggaaata cccgtgccgc caagtccggc gacgaggatt tttttgtttt
    18781 ggaaagtcat tttggtttgt cctaaaacaa atcatattgg gcagggggat gtctactcct
    18841 gctcaagccg ctttcagacg gcattgcgag ctgttcaata acccgctttc aggcgttggt
    18901 cattgtcgca gccgtcttgg tttccgtttt gacacgcctt gccaagccat tcttgtgcaa
    18961 gggcgcggtc ttggcgcacg ccgtgccctt tgtaatacat tgcgcccaaa ttaaattggg
    19021 cttgtgctaa cccctgatcc gccgcctgcc gaaaccatct gaccgcttcg gtatcatctt
    19081 ggcgcacgcc ttgtccttcg gcatacatcg cgcccaaatt gttttgggca tcagcatctc
    19141 cctgctctgc cgcctgcaag ttttcccgaa aatccgatac gtcatccgcc cacaccgctt
    19201 ggttcaagcc caaggcaatc agggcggcgg caagccattt gactgtctgt ttcatgtttt
    19261 ctctcctatt tcggtgtggg gcggtttttc agccaccgtt aacgatagga cgggacagat
    19321 tgccgccgca ggtttattgc gcgttcaaat gccgtctgaa aggtgttcag acggcatagg
    19381 ttcagcggat tttgagggta ctcaaaccga tcaataccaa gacgatggta ataatccaaa
    19441 agcggacgac gacttgggtt tctttccagc ctttttgttc gtagtggtga tggatgggcg
    19501 ccatcaggaa gatgcgtttt ttggttttct tataccagcc aacctgaagc ataacggata
    19561 cggcttctac gacaaataat ccgcccataa tgacgaggac aaactcttgg cggacgatga
    19621 cggcgacggt accgagcgcg gcacccaatg ccaatgcacc gacatcgccc ataaagactt
    19681 gcgcgggata ggcgttaaac cacaagaaac cgaggcacgc gccgcacatg gcggtacaga
    19741 atatagcgac ttcgttcgca ccggcgacat agggaagctg gaggtattgg gaaaattggt
    19801 agtgtccgct gacgtaggcg aaaatggcga ggccggcagc aacgaggacg acggggaagg
    19861 tcgcaaggcc gtccaagccg tcggtgaggt tgacggcgtt ggatgtgccg acgatggtca
    19921 ggtaggacaa caccaaaaag ccgaccacgc ccagcggcag ggcgatttgt ttgaagaacg
    19981 ggacaatcaa aatattgttg gcggaattgg cggcaaggta aaacaatgcc aaaccggcga
    20041 taatggcaac gcttgactgc cacaccattt tgaatttggc ggacacgccg ttggggtctt
    20101 tatagacgac tttgcgccag tcgtcgtaaa aaccgagcgc accggtggca agcagtacgc
    20161 ccaagagaat ccagatatac gggttcgccc agttgcccca caacagagtg gacacggtaa
    20221 tggcggtcag aatcagcgaa ccgcccatcg tcggcgtgcc gtttttgacg aggtgggttt
    20281 gcgggccgtc ggtacgcact gcctgcccgc atttgagcgc ggtcagcctg cgtatcgtcc
    20341 acgggccgaa catcagggaa aacgccaagg cggtcaacgc cgccatgacg gcgcggaatg
    20401 tggtgtattg aaaaatattc agaccggtta accagttgct gaaatgtgcg agccataaaa
    20461 acatggggct tccttttttt gttttgt
//
LOCUS       DAWKQJ010000019        20276 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000019 DAWKQJ010000000
VERSION     DAWKQJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.019"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            78..1262
                     /gene="coaBC"
                     /locus_tag="WJL20_000525"
     CDS             78..1262
                     /gene="coaBC"
                     /locus_tag="WJL20_000525"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ7682710.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRCITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
     gene            complement(1329..3485)
                     /locus_tag="WJL20_000526"
     CDS             complement(1329..3485)
                     /locus_tag="WJL20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7682711.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTIDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3578..3784)
                     /locus_tag="WJL20_000527"
     CDS             complement(3578..3784)
                     /locus_tag="WJL20_000527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ7682712.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3843..4460)
                     /gene="gmk"
                     /locus_tag="WJL20_000528"
     CDS             complement(3843..4460)
                     /gene="gmk"
                     /locus_tag="WJL20_000528"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ7682713.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGIHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4588..5187
                     /locus_tag="WJL20_000529"
     CDS             4588..5187
                     /locus_tag="WJL20_000529"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ7682714.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5233..6021)
                     /locus_tag="WJL20_000530"
     CDS             complement(5233..6021)
                     /locus_tag="WJL20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ7682715.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6346..7701
                     /gene="yegQ"
                     /locus_tag="WJL20_000531"
     CDS             6346..7701
                     /gene="yegQ"
                     /locus_tag="WJL20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ7682716.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            7835..8311
                     /locus_tag="WJL20_000532"
     CDS             7835..8311
                     /locus_tag="WJL20_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ7682717.1"
                     /translation="MLAVCQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8564..8905)
                     /locus_tag="WJL20_000533"
     CDS             complement(8564..8905)
                     /locus_tag="WJL20_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7682718.1"
                     /translation="MPQVKLIITRPAQSDLVRIYNFLNDLGAEHQANTVMRLLKNSFV
                     STQGSPSNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFR
                     ENAYRLSFPFL"
     gene            complement(8893..9180)
                     /locus_tag="WJL20_000534"
     CDS             complement(8893..9180)
                     /locus_tag="WJL20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682719.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVDGLGGDESKDMPECRK"
     gene            9250..9360
                     /locus_tag="WJL20_000535"
                     /pseudo
     CDS             9250..9360
                     /locus_tag="WJL20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(9550..10794)
                     /locus_tag="WJL20_000536"
     CDS             complement(9550..10794)
                     /locus_tag="WJL20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7682720.1"
                     /translation="MRLRSNPRKHRQIKLPPPPTDTGNPATGADTASSSAGGDGGDSK
                     TEIPPKDTQGEGASAGTDGGVQNDAPQHNDNAPAAGTSDTQPENNRDMPGSDAGRADG
                     GNQDTDAPKNAESQDGTEGNAVSEGTGMPANPSSENAEHTEVATNTEGANPPSEAEDS
                     KDAGQDGMQADEASGRQDAEEGLQGDDPAAHPPQDQTPKNAADNQPKTGVFGEKYFDS
                     TGEITALVIGGETIDLTKADEPVSSKNHPGLNSDFDKVMVSQKQYKHLIYGAAKLEDG
                     TYTLFVQGKPIEQLPTGMAKYKGNVLHFVGKYKDDWLNNSAYKAAGTFTADVDFSGKT
                     LTGKIDTKDPSGMGMQEFAANIDGNGFKGKWKSDNDKGDISGRFYGQDGAEMAGRYDY
                     KNQDDRKDGFGVFAGKKEVSTR"
     gene            complement(11006..11635)
                     /locus_tag="WJL20_000537"
     CDS             complement(11006..11635)
                     /locus_tag="WJL20_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ7682721.1"
                     /translation="MSETENQALTFAKRLKADTTTVHDSVDNLVMFVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     AGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            11810..13270
                     /locus_tag="WJL20_000538"
     CDS             11810..13270
                     /locus_tag="WJL20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ7682722.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRFFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDSGEAAFCPR
                     CGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRAMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
     gene            13263..14924
                     /gene="pqiB"
                     /locus_tag="WJL20_000539"
     CDS             13263..14924
                     /gene="pqiB"
                     /locus_tag="WJL20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ7682723.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTAFNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            14924..15442
                     /locus_tag="WJL20_000540"
     CDS             14924..15442
                     /locus_tag="WJL20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ7682724.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            15446..15997
                     /locus_tag="WJL20_000541"
     CDS             15446..15997
                     /locus_tag="WJL20_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ7682725.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            15987..16628
                     /locus_tag="WJL20_000542"
     CDS             15987..16628
                     /locus_tag="WJL20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ7682726.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(16621..16851)
                     /locus_tag="WJL20_000543"
     CDS             complement(16621..16851)
                     /locus_tag="WJL20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7682727.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            16799..17158
                     /locus_tag="WJL20_000544"
     CDS             16799..17158
                     /locus_tag="WJL20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682728.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVLAGFMVLVSGFPLSLLLVVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            17189..20041
                     /gene="gcvP"
                     /locus_tag="WJL20_000545"
     CDS             17189..20041
                     /gene="gcvP"
                     /locus_tag="WJL20_000545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ7682729.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLETLGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFDFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
BASE COUNT         4900 a         5596 c         4936 g         4844 t
ORIGIN      
        1 tccaaacggt aactaatgta tccgcacttt gttatagaat ggctgctgtt ttttcttcgt
       61 aattagaaat tgtcaaaatg ggtaaacata ttcttttagg tgtaacgggc agtattgcgg
      121 cgtataagtc ttgcgagttg gttcgtctgc tgaaaaaaca ggggcattcg gttacggtgg
      181 ttatgagccg ctcggcaact gaatttgttt ctccgctgac ttttcaggct ttaagcggca
      241 atcctgtcct gaccgacacg cacggcggca acggttcaaa cggtatggaa catatcaacc
      301 tgacccggaa tgcggatgtt tttctgattg cgccggcaag tatgaatacc gtggcaaaaa
      361 tctgtaacgg cgtggcagat aacctactga ccagtctggc agccgcacgg aaatgtccgc
      421 tcgccatcgc tcccgcgatg aatgtggaaa tgtggctcaa ccctgccaac caacggaata
      481 tcgcacaact ggtttcagac ggcattactg tctatatgcc gggcttgggc gaacaggctt
      541 gcggagaaaa tggtatggga aggatgccgg aacctgccga attgctggat ttgcttccgg
      601 acttgtggac accgaaaatt ttaaggggca agaaaatctt gattaccgca ggtgcgacat
      661 ttgaagccat tgaccctgtc aggtgcatca caaatacctc cagcggaaaa atgggggtag
      721 ctttggcgcg ggcgtgccgt gccgccggtg cggaagtcag cctgattcac ggacagcttc
      781 aaaccgcgct gcctttcggc atatccgata cggttcaagc cgtcagcgct gaagatatgc
      841 atcgcgcagt gcatcgtttg attgaaaaac aagatgcttt tatttctgtt gccgccgtct
      901 cagactataa ggttaaaaac aagagtacgc aaaaattcaa aaaagataaa aatgccaaac
      961 cgttatccat cgaattggat gagaaccccg atattttggc ttctattgcc tcattgccga
     1021 acccgccgtt ctgcatcggt tttgccgctg aaacggagaa tgtaatggca tatgcacgag
     1081 aaaaacgtat taagaaaaaa ataccggtga tagttgccaa tgatgtttca atcgcgatgg
     1141 gcaaaccgac caaccggatt accattatcg gggacgacgg ggaactatct tttcccgaaa
     1201 caagtaaaga tgaagcggcg atgcagattg ttgaaaggct tgccggatat ttgaacaaat
     1261 aagcaattga acggataaac cataaagcgg gttgcctgtt aatcaaaagg caacccgttt
     1321 tacctgcttc aacttctgat gactttgcgg atatatggaa tactatgcag attttgaata
     1381 atctgattca attgattcag attcttgact ttcaataaga atttgaattc gacaaaacct
     1441 tccgttcccg actgggattt agacggtgtt tcgaccgact caatgtccgc accggaatcg
     1501 gaaatcgctt gcgccattaa tgccaacagg ccgtggctgt cttccgattg gacttgaagc
     1561 ccgacacggt agttctgccc gttcatattt tcccagtctg catccagctg ctgttcggga
     1621 tcggacttca acaacgtcgg gcaggtatct ctgtggataa tcatgccttt tcccttaacc
     1681 aacagcaaac ggatggaatc accaggaaca gggtggcagc actctgcaaa atgaatatgc
     1741 ccgctttcct gaccatcaac tttaatggaa ctgagcctga cctcgctgcc gaaatgctcc
     1801 cctgccaact cggcaatgtg catggcgaca taaacaggca gggtatgccc cataccgacg
     1861 ttgtacagca cttcctcaaa tgaagtctgt ttgtcgttga gatcggcaag gtatttttct
     1921 ttgatgccgt ctgaaagcag gacatctttg ggcagcaaac tggacagggc tttttgtaag
     1981 aggctctctc ccaaaacgac cgcatcgtgc cggttaaggt ttttaatata ttggcgtatg
     2041 gcactgcgcg ccctgcctga cacggcgaaa ttcaaccatg caggattggg tttggcatgt
     2101 tcggatgtaa taatttcgac ggaatcgcct gttttgagct tcgtacgcaa cggcatcatg
     2161 atattgttga tacgtgcggc aacggttttg tgcccgatat cggtatgcac cgcataagca
     2221 aaatcgacag gcgttgcccc tttgggcaaa gttaggattt ttccttttgg cgtaaggatg
     2281 tagatttcgt tcggaaacaa atcgactttg acgtgttcga gaaactcaat ggcattggca
     2341 ctgcttgcct gcaaatctaa gatatttttc agccaccggt ttgtgtgaag caccgcctga
     2401 tcgatcgtct tagaatatga tttatagctc caatgtccgg cgattccacc ttcggcaaca
     2461 gcatccattt ccttggtacg tatctgaact tcaatcggca agccgtaagg gccgaccaaa
     2521 gtcgtatgca gactttgata cccgttgctt ttcggaatgg cgatatagtc tttgaaccgc
     2581 ccgggcttgg gctgatagag ggtgtgcaat gcgccgagtg cggcataaca ggctggaatg
     2641 ctgttgacaa tgacgcggaa accgtaaata tccataacct cggcaaagcg cagctttttc
     2701 gccatcattt tctgatggat gccgtacagg tttttttccc tgcctttgat tttggcctct
     2761 atattcgcgc ctaccagccg ctggccgaat gcgcgcaaga ctttgccgac aacgtcctgc
     2821 cggttcttcc ggctcttgtc catcgctttt tttaaagtct cgtagcggtt gggatgcagg
     2881 ttttggaacg ataaatcctg aagctcttga tatgcgttat tcaaacctat acggttggca
     2941 atctgtgcat agatttcaag ggtttccctt gcaatccggc ggcgtttgtc cgggcgcatc
     3001 gaaccgagtg tccgcatatt atgcagacgg tcggcaagtt taacgacaat cacgcgcaca
     3061 tctttggtca ttgccaaaat cagtttgcgg aaactctccg cctgatgttc cgcatgatct
     3121 tcaaatttga gtttttcgag tttggacaga ccgtccacca tctcggcaat cgtattgccg
     3181 aacaccgccg ccatttcccc ttttgtcacg cccgtatctt ccaatacgtc gtgcatcacg
     3241 cctgcacaca gaccctgtat gtccatatgc caaagggcga gctgcgtcgc aacggcaatt
     3301 ggatgcgtaa tgtatggttc gccacttttc cgggtttgtc cgtcatgagc gtgaaatgca
     3361 taggcaacgg ccttctcgag ctcaacctgt tcttcaggct tgagatagga agcggtacgg
     3421 aaaagcaagg tgcgtgcttc ggcagttaaa tggtcgtaag gtgcggtggg ttgagggaca
     3481 ggcatttcag acggctttcg gaacatattt tatttttcgt ttcaaaccgt cggactgcac
     3541 ggcggcagaa tgtgccgacg tgcgtttccg gcagaattta tttattgcgc gtcaacagtt
     3601 ccgtaccgat atgcccggcg gcgatttccc ttaaggcggt aacggtcggt ttgttattgc
     3661 ggacatcgtc cacaagcggc gtattgccat tttcaagctg gcgggcgcga cgggccgcta
     3721 ccaatgtcag gtcaaaatgg ttggaaattt ttccggtaca gtcttcggtg gtaatacgtg
     3781 ccatattatt tgctttcttt caaaaatatt taaaatagga aactgggtat tttcgcggtt
     3841 ttttaggaat tttccaacaa atctgcaata aatcccagtt gccgcgacct tttcagacgg
     3901 caggcattca caatatggcg caaatcctcc tccgcccgcg ccaagtcgtc attgaccacg
     3961 acaaagtcga acaatacgga ctgttcgatt tcatgccttg ccttcgcaag cctcctttgg
     4021 ataacttcct gactgtccgt cccgcgtccg ttgagacgcg cggcaagcac atcgaaagac
     4081 ggcggcagga taaagatgcc gacagcttca ggcagcgcat ctcgaacctg cgccgcgccc
     4141 tgaacgtcga tttccaaaat cacgtcataa cctgccgccg ccaacgcatt cacgccctcc
     4201 gcgcttgtgc cgtaatagtt gccgaatacg tctgcgtatt ccaaaaaagc ctcctgtgcg
     4261 ataagcgact caaactcttc tttggaaaca aagtgataat gtatgccgtt tgcttcgcct
     4321 tcacgcggcg ggcgcgtcgt gtgcgacacg gaaacgcgca aaccgttatg gtttgccaac
     4381 agccgcgaca ccagcgtggt tttgcccgtg ccggaagcgg ccgaaatgat aaagatgttg
     4441 ccttttcgat aagcggacat attttttacc tgtatatttt ccagccgatt gtatcacaat
     4501 ggacacccag tttcctattt gccgatgccc atattttgcc gctattgttt tgattgattt
     4561 ggcaagcggc aggctgacgg ctacaatatg gcgttaaaaa catcaaactt ggaacacgca
     4621 atgctggttc atcccgaagc tatgagtgtc ggcgcgcttg ccgacaaaat ccgcaaaatc
     4681 gaaaactggc cgcaaaaagg catcttattc cacgacatca cgcccgtcct gcaaagtgcg
     4741 gaatacttcc gccttttagt tgatttattg gtttaccgct atatggatca gaaaatcgac
     4801 atcgttgccg gtttggacgc gcgcggcttc attatcggcg cggcactcgc ctaccatctc
     4861 aacgtcggtt ttgtccccat ccgcaaaaaa ggcaagctgc cttttgaaac cgtatcgcaa
     4921 agctacgcgc tcgaatacgg ggaagctgcg gtggaaatcc acaccgatgc cgtcaaaccc
     4981 ggttcgcgcg tgctgctggt cgatgatttg gttgccacgg gcggcacaat gcttgccggg
     5041 ctggaactga tccgcaaact cggcggagaa attgtcgaag ccgccgccat tttggaattt
     5101 accgaccttc aaggcggcaa gaatatccgt gcaagcggcg cgcccttatt taccctgctt
     5161 caaaacgaag gctgtatgaa gggctgaaaa ccgaccctgc cgtctgaaac cggcagggtt
     5221 gttatgatgc gttcaaatca cgcccaaatc ttgcaagccc ctcaacacgc cgtcttcatc
     5281 aacgccgggg caaacatatt tcgccgcctc tttcactgcc tgttccccgt tacccattgc
     5341 cacaccgaac ccgacttcag acagcatttc cacatcgttc aaaccgtcgc cgaacgccat
     5401 cacgtccgcc atttccaaac ccaatgcttc aaccacgctt ctgatgccgt ctgttttcga
     5461 cattcccgca ggcagcagat cgaccgcttc ctcgtgccag cgcaccgttt tcaagccttc
     5521 ccgttccaca atatccgacc agggcggcat ttcgttttcc tccgcaaaca ccagcatctg
     5581 atacaccggt ttgcttgaaa aataatcctt atcggcaaaa aaatcgctgg cgatatgctg
     5641 caaggcgcgg cacacgcatt ccgacagcgc ggacacggcg atcccctctc cgccgacaaa
     5701 cgcataatcc atacccaagc catccaaatg cgcgcaaacc ctgcccatca aaccggcatc
     5761 catcggtact tcgcgcacag tttttccgtg cagcagcgca aactgtccgt ttatcgttac
     5821 cacggcatcc atgcccgttt ccgccatcat gtccctgacc ttttcgggaa tcgtcgccaa
     5881 agaccgcccc gttgccaacg ccgtcaatat gcctttgccg cgcaaagccg ccaccgccgt
     5941 tttcacggaa ggacgcaagg tatccgtata tttgcggtac agcgtatcgt caatatcgaa
     6001 aaacacgatt ttaggattca tcacattctc tctcgcattc aaactgccgc attgtatccc
     6061 aatcaggcaa atacttgata aatccttata aatttcccgg caaaattgac cgcgaacacg
     6121 aaaaggcgga taatccgccc atcctcaaac ccttttcaga cggcatttgc agcaatgccg
     6181 tctgaaacat ttttacaaag catacaaatc atgtttcaac acacaggacg acacataaag
     6241 cgtcgcccta tgtgttgccc tgattcggaa ggggttacgc ccctcccaaa taaagtctga
     6301 ttctaccgcc ctaaagggcg gggtttcaac cgaaaaggaa acacgatgaa agcacccgaa
     6361 ctcttattgc ccgccggcgg attggaaaga atgcgcaccg cctacgacta cggcgcagat
     6421 gccgtttacg ccggcagccc gcgttattcc ctgcgcgccc gcaacaacga atttgccaaa
     6481 cttgatgttt tagaacaagg cattaaagaa gcgcacgagc gcaacaaaaa attctttttg
     6541 accgtcaaca ccctgccgca caattccaaa ctcaaaacct ttgtttccga tatggagccg
     6601 ctgattgcga tgaagcccga cgcgctgatt atggcggatc cgggcttgat tatgaccgtg
     6661 cgcgaaaaat ggccggaaat gccgatccac ctgtccgtac aggcgaacac caccaactac
     6721 tggggcgtga aattctggca gaacatcggc gtcgaacgca ttattctgtc acgcgaattg
     6781 agtatggaag aaatcgccga aatccgccaa caatgccccg acatcgaact cgaagtcttc
     6841 atccacggcg cattgtgcat cgcctactcc ggccgttgcc tattgtcggg ctatttcaac
     6901 caccgcgacc ccaaccaagg cacttgcacc aactcttgcc gttgggatta caaagtccac
     6961 aacgctacgg aaagcgatgc gggcgatgcc cagcttctgc aaggttttaa ctttgaaaaa
     7021 gcccaagaag aagccaacca aaacttcgaa ggcatcaacg gtcagaaacg ccatccctac
     7081 gccgacaagg ttttcctgat tgaagaatcc aaccgtccgg gcgagatgat gccgattatg
     7141 gaagacgaac acggcaccta catcatgaac tccaaagacc ttcgtggcat tgaagtggtt
     7201 gagaaactcg ccaaaatcgg cgtggacagc ctcaaagtcg aaggccgcac caagtcgctc
     7261 tattatgtcg cacgcgtcgc ccaatcctac cgcaaagcga ttgacgatgc cgtcgcaggc
     7321 cgtccgtttg attacagcct gttgagcgaa ctcgaaggcc ttgccaaccg cggctacacc
     7381 agcggcttcc tcgaacgcca ccacactcag gattatcaaa actacctgac cggccattcc
     7441 accgccaaac aaagccaata cgtcggacac gttaccgaaa tcgacaaaaa cggctgggcg
     7501 accatcgaag ttaaaaaccg cttcgccgtc ggcgattcac tcgaaatcat ccacccgaac
     7561 ggcaaccaaa ccatcaaatt ggaacaaatg gcccgaaaag gccagcctgt cgatgtcgca
     7621 cccggcaacg gcatccaggt caaaatcccc aatatgcggg gcaaagaaaa agccctcatc
     7681 gcacgcgtat tgaacccctg agccacaatg ccgtccgaac cccgttttca gacggcattt
     7741 tgttgaatct tacggtacaa tggcaatgat ttcccaccgc aagcaaggat atccgacatg
     7801 aaaaaaaatc ctcatactct ccgccgccgc cctgatgctg gcagtctgcc aatccggcag
     7861 cgcacctaaa aacgcgcaca cggcatcccc cggcacaccg gccggagaat catgcaaagt
     7921 catccggggc gacaccgcag gaagcggcaa aaacatcatt taccgttgct gcaacggtca
     7981 ggcggcaata aatgcagcca ttgcagacgg cgtattggaa tcagggattc ccgtcagctt
     8041 cggcggcggg ggcgtaccgg ttgcaaacgg caggaatcta gttacccgtc aggcggcaaa
     8101 cggcataggc aaatccgact cagaagccta cgaacgcgcc ctaatcaatg ccgcccgcaa
     8161 attccaacag accgcaggca aactcggcgg acgcggcgtt accggcttcc acagctactt
     8221 tgacaagcaa ccgctgcaag gcgggcaata cgactgtcag gcaggctcgt tccacgtccg
     8281 cgtcgtcatg cgcgccaatg tcgtccgtta acatatcggc aatcaaaaaa tgccgtctga
     8341 aacatttttc agacggcatt tttaatcccc ttgccttatt gtgcggcaga ttcagatcgg
     8401 gacactccat acttccatat ctgcaccgtt caaacatccc gcctgcggaa cggaatcaaa
     8461 gcggcagtcc ggttatgttc ttgaaggcgg tttatccgaa cgtgccgcaa caaagccgaa
     8521 acgcattgcc ccgcacaagt gaaagccgcc aaaccaaata ccgttataaa aaaggaaagc
     8581 tcaggcggta ggcattttca cggaaatgcc tcaccgtcaa aatcaatatt tgcttacctg
     8641 atttatcgtg ccaaaacaaa tagctataac cgcttttgcc ataaggcacg attacttccc
     8701 ttacattttg caaagtttcc cccgccaccg acaaatcata aggtttccca ttactcgggc
     8761 tgccttgtgt agaaacaaaa gaatttttaa gcagccgcat cactgtattt gcctgatgtt
     8821 ccgcacccaa atcattcaaa aaattataaa tcctgaccaa atcagattga gcggggcgtg
     8881 taattatcag ctttacttgc ggcattcggg catatccttc gattcatcgc cacccaagcc
     8941 gtccacccag tctgcaaact cattaagcgt aatatgcaat cctgtttccc gataatcctg
     9001 aaaagcctgc atcgcccgct tatcgtaggc ttgtttggct ttcaagcgtt ctatatgacc
     9061 atccaccgca tccaatacga tactgtgcac actgcccccg aaatcccggg ccagctcttg
     9121 taaaacggct ttgcggtatc ccagtttgac accggttact tcctgattgg taagcatcat
     9181 tattttccca taaatcaaac gtctgacacg gcattataaa cacaatgcgg catctgccgc
     9241 cacccttgcg gacgcggcgt taccggcttc ctcagctatt tcgacaagca gccgctgcaa
     9301 ggcggacaat acgactgtca ggcaggctcg ttccacgtcc gcgtggtcat cgtccgttaa
     9361 acatatcggc aatcaaaaaa tgccgtctga aacatttttc agacggcata gttcataaat
     9421 caaatcggta atacagataa tcgttcaatt aggattgctt ctgcaggtgg gacaacacat
     9481 caaggtgttg cccgatgccc tgtcggaagg aaacgccact gtttccgact gtgaaacaca
     9541 ggataaatat caacgggtgc ttacttcttt tttgcctgca aatacgccga agccgtcttt
     9601 tctatcatct tgatttttat agtcgtatct gcctgccatt tccgcgccat cttgaccata
     9661 aaatctcccg gaaatatcgc ctttatcatt atccgatttc cacttacctt taaacccatt
     9721 tccatcgata ttcgcagcaa attcttgcat ccccatacct gaaggatctt tggtatcaat
     9781 ttttcctgtt aaggttttcc ctgagaaatc aacatctgcc gtaaacgttc ctgcggcttt
     9841 atacgcacta ttattgagcc aatcgtcctt atattttccg acgaaatgca acacattacc
     9901 tttatattta gccattcctg tcggcaattg ttctatcggc ttaccctgta cgaaaagggt
     9961 gtatgtaccg tcttctaatt tagctgcccc ataaatcagg tgcttatatt gtttttgaga
    10021 aaccattact ttgtcaaaat cactgttaag acccggatga tttttacttg ataccggttc
    10081 atcagctttc gttaagtcaa tagtttcccc accaatcacc aacgccgtga tttctcccgt
    10141 actgtcaaaa tatttctcgc caaacacgcc tgttttaggt tggttatcgg cagcattttt
    10201 gggggtttga tcttggggag gatgggcagc cgggtcgtcg ccttgcaacc cctcttccgc
    10261 atcctgcctg ccacttgctt catcagcctg catcccgtct tgccccgcat ctttggaatc
    10321 ttccgcttcc gacggcgggt ttgcaccttc ggtattcgtc gcaacctcag tatgttcggc
    10381 gttttcagaa gaagggtttg ccggcatccc tgtgccttcg cttacagcgt ttccttccgt
    10441 accatcttga gattccgcat ttttcggcgc atcggtatct tgattgccgc catccgccct
    10501 tcctgcatca ctgccgggca tatcacggtt gttttcaggc tgcgtgtcgg atgtaccggc
    10561 tgccggcgca ttatcattat gttggggcgc atcattttgc actccgccat cagtgcctgc
    10621 cgacgcgcct tccccttgcg tatctttagg cggtatttct gttttagaat ccccgccatc
    10681 cccacctgcc gaggagcttg ccgtatcagc acctgtagca gggtttcccg tatcagtggg
    10741 gggggggggc aactttattt gacggtgttt ccgaggattt gaccgcaggc gcatcccctc
    10801 ctcctgaaat acaggcggaa agggcaagga cggatgccat tgcaagcagg cttggtttga
    10861 aaggaatcgt cataattatc tcctaatggc atatttttaa taaaaaatat gataattatt
    10921 tatatttgat ggtttgaaaa atgccgtcat acgcggcaga aaatacggtt ccgcttcacg
    10981 agccgaggcg aaaccgtaaa atatatcaat gcctgtgcgg catcatacct tcgggggctt
    11041 cggcatcggc tgccaagccg aaggtttcgc gcaacacgac tttgtagaat gcaaatgctt
    11101 cgcgcgcgcc ttggatggct tccgcttcgg cttcgggagt caggttcaag gcgttcagat
    11161 gctcgacgaa ggcgcgccag tgtttgccgc gcccatcggg atggggtgcg aggtggcgcg
    11221 cgccgtgttc gccgttgtaa tcgagttttt gggcgtgttt gaacaaaaac gccgcgccca
    11281 aattggatcc ttcggcgcaa taaagccagc cgattgcttt gttgccggct tcgtatggca
    11341 attctttgtc gaatttgtaa ggctcttcgc ccaagtcttt caagtcttgc gttacggcat
    11401 cgtatcgcgc catatattcc agctcgggaa tggctttgtt taattcggca tctttataga
    11461 tatgatcgac ggccttgtgg aaaacggatt ggagtttcaa aaatttgatg tagttttctt
    11521 tgctgacaaa cggttggaca aacataacga gattatccac gctgtcgtga acggtggtgg
    11581 tatctgcttt caagcgtttg gcaaacgtca gggcttgatt ttcggtttcg ctcatagctt
    11641 ttcctttgtc ggtaaggggt aaggatgcgg ggcgggcaaa atccgtacct gaccgcatac
    11701 aaaaataata aacgagattt attatcacat atttttggaa aaaatatcat ttgcgtgatg
    11761 tttttaagca ggtattttac tattctttac agaatcggga ttttatcaaa tgggttcggc
    11821 agtcggcgga cagccgctcg aaaaaatatt tttgccggac gacaagggct tgttcatact
    11881 gccgaatctg ccggttttgc atcctgattg ggtgtatcgc tttttttcct ttataatgcc
    11941 gccacttata tttgccactt tcccgatgaa gccgtttgcc gaaaatatcc cacacagcct
    12001 tcgcggcaac tgccgcgacg aagccctgcc gccgcatacg gtagattgcc cggaatgcgg
    12061 ctgccgcgcg gatgtacctc ggttggacag tggagaggcg gcgttctgtc cccgttgcgg
    12121 acacaaactc ttcagggtgg gcaggcatcc tttttccgcc ccgcccgcct atgcggcggc
    12181 ttcgctgatt ttaatggcgt ttgcttacgg tatgacgtat atcgaggtcg ggataccggg
    12241 tgcggcatcc gtcctttcgc tgcccgagat gatgcgcctg atggtgtttc aggattatgg
    12301 ttttttggcc gaagtgatgt ttgtgctgac tttcggcgcg ccggttctgt ttctgctgct
    12361 gtgcctgtat gtctatgccg cgctgatacg gaaacaggcg tatcctgcgc tgcgtttggc
    12421 aacgcgtgcg atggtgcgct tgaggcaggc gatgatggtg gatgtgtttt ttgtttccac
    12481 tctggtggcg tatatcaagc tctcgtctgt ggcagaggtt cgcttcgggc cggcgtttta
    12541 tctgatgttc gcgctgtcgg ttatgctgat tcggacttcg gtatcggttc cccagcattg
    12601 ggtgtatttt caaatcgggc ggctgacggg ggataatgcg gttcagacgg catcggaagg
    12661 caaaacctgt tgcagccgct gcctgtattt ccgcgacagt gccgaatccc cctgcggtgt
    12721 gtgcggcgcg gaactgtacc gccggcggcc gaaaagtctg agtatttcgt cggcgtttct
    12781 gacggcggcg gttattttgt atttccctgc caatatcctg ccgattatga tttcgtccaa
    12841 tcctgccgcc acggaggtca ataccatcct taacggcatc gcttatatgt gggacgaggg
    12901 cgacaggctg attgcggcgg ttattttcag cgcgagtatt ttggtgccgg tactgaagat
    12961 tgcggcaatg tcggttttga ttgcgtccgc ccgcttcgct ttgccaacgg gcgcaaagaa
    13021 attgtcgcac ctctaccgca tcaccgaagc ggtcggccgc tggtcgatga ttgatatttt
    13081 tgtgattatt attttgatgt gttcgttcca cacttatgcc gcgcgcgtca ttccgggcag
    13141 tgcggcagtc tatttctgcc tggtcgtgat tctgacgatg ctgtccgcct attatttcga
    13201 cccgcgcctg ctttgggaca aacgcgcttc agacggcatt gctttcaatg aaacggaaaa
    13261 acatgactga caacagccct cctccaaacg gacacgccca agcacgcgtc cgcaaaaaca
    13321 acaccttgct ctccgccgtc tggctggtcc cgctgattgc gctgattgcc ggtggctggc
    13381 tgtggattaa ggaaatccgc aacagggggc ctgtggttac gctcttgatg gacagcgcgg
    13441 aaggcattga ggtcaacaat acggtcatta aggtattgag catcgatgtc ggacgcgtta
    13501 cccgaatcaa actgcgcgac gaccagaaag gcgtggaagt aaccgcccaa ctcaatgcgg
    13561 acgtatccgg cctcatccgc agcgataccc agttttgggt ggtcaagccg cgtatcgacc
    13621 aaagcggcgt aaccggtttg ggtacgctgc tttcgggttc gtacatcgct tttacacccg
    13681 gcaaaagcga cgaggcaaaa gacgtgttcc aagtgcagga cattccgccc gttaccgcca
    13741 tcgggcaaag cgggctgcgc ttgaatttga ttggtaaaaa cgaccgcatc ctcaacgtca
    13801 acagccctgt tttgtatgaa aactttatgg tcgggcaaat cgaaagcgcg catttcgacc
    13861 cgtccgacca aagcgtgcat tacaccatct tcatccaaag ccccaacgac aaactgattc
    13921 attccgccag ccgtttttgg ctggaaagcg gcattaatat cgaaaccaca ggcagcggca
    13981 tcaaactcaa ttccgcccct ctgcctgccc tgctgtcggg cgcgatttca tttgattcgc
    14041 cgaaaaccaa aaacagtaaa aacgtcaaaa gcgaagacag cttcacgctt tacgacagcc
    14101 gcagcgaagt cgccaacctg cctgacgacc gctcgctgta ctacaccgcg tttttcaaac
    14161 aatccgtgcg cggactgacc gtcggttcgc ctgttgaata caaagggctg aatgtcggcg
    14221 tggtttccga tgtcccttat tttgaccgca acgacagcct gcacctgttt gaaaacggct
    14281 ggattcccgt gcgcatccgc attgagcctt cccgtttgga aatcaatgcc gacgaacaaa
    14341 gcaaagagca ttggaaacaa caatttcaga cggctttcaa caaaggcctg accgccacca
    14401 tctccagcaa caacctgctg accggcagca aaatgattga gttgaacgat cagccttcgt
    14461 cttcgcccaa gctgcgaccg cataccgttt atgcaggcga taccgtcatc gctacacaag
    14521 gcggcggttt ggacgatttg caggcgaaag tggtggattt gctggacaag ttcgacaaac
    14581 tgcctttgga taagacggtt gccgaattga acggttcgct tgccgagctc aagtccgcac
    14641 tcaaatctgc caatgccgcc ctaagctcca ttgacaaact ggccggcaat ccgcagacgc
    14701 aaaacattcc gaacgagttg aaccaaaccc tgaaagagtt gcgcacaacc ctgcaaggcg
    14761 tatcgcctca atcgcctatc tacggcgacg tacaaaatac gttgcaaagt ttggacaaaa
    14821 ccttaaaaga cgttcaaccc gtaattaata ctttgaaaga aaaacccaac gcgctgattt
    14881 tcaacagcag cagcaaagac cccatcccga aaggaagccg ataatgcgcc tcttcccgat
    14941 tgccgccgcc ctgacgcttg ccgcctgcgg tactgtgcaa agcacacaat atttcgtgtt
    15001 gcccgacagc cgctacatcc gtcctgcaac acaaggcggc gaaaccgccg tcgaagtccg
    15061 tcttgccgaa ccgctcaaac gcggcggact ggtctatcaa accgacccct accgcctcaa
    15121 caccgcacaa aaccacgttt gggcagacac cttggacgat atgctcgaag cggcgttgag
    15181 caatgcattc aaccgtttgg acaacacacg catctttgtt cctgcctcac gcagcggcag
    15241 taccgaaaaa tggacggtct atatcgacgc attccaaggc agctacacgg gcaaaaccct
    15301 catcagcggc tacgccgtcc tacccgacgg tacgaacaga cccttccata tcgaaaccga
    15361 acagcagggt gacggctacg ccgcgatgac cgccgcactc gaacagggac tgaaacaggc
    15421 ggcgcaacag atggtcgagt aaaccgtgaa ctattgcgaa tttgccgcct cacttcccga
    15481 aaacaccgat aacccgaaca aacattacca cgacacgcaa tacggttttc cgattgagga
    15541 cgacaatgaa ttgtttgagc ggctggtgtt ggaaatcaat caggcaggat taaactggac
    15601 gctgatgctg aagaagcggc aggcgtttca gacggcattt gaaggtttcg acatcgatac
    15661 ggttgccgcc ttcgacgaca ccgaccgcga acgcctgctt gccgacgcgg gcattgtccg
    15721 caaccgcctg aaaatcgatg ccgccatttt caatgcacgg caaatccaag cgttgcaaca
    15781 agaatacggc tcgttcaaga actggctcga cacgcaccat ccgcgaagca aagacgaatg
    15841 ggttaaactc tttaaaaaac atttcaaatt cgtcggcggc gaaatcgtcg gcgaatttct
    15901 gatgagtacc ggctacctca aaggcgcgca cgccgaaagc tgtccggttt accgtgaaac
    15961 cctgaaatac cacccgaaat ggctcgatgc catctgaaaa accaatgaac agaagaacct
    16021 tcctcctcgg cgcaggcgcg ttgctgctta ccgcctgcgg cagaaaatcc gcccgaaccc
    16081 acgccaaaat tcccgaagga agcaccgtac ttgccttggg cgattcgctt accttcggct
    16141 acggcgcaaa ccctggcgaa tcctaccccg cgcaactgca aaaactgacg ggttggaata
    16201 ttgtcaacgg cggcgtatcg ggcgatacat ctgcccaagc cctgtcgcgc ctgcccgcgc
    16261 tgttggcacg caaacccaag cttgtgattg tcggcatagg cggcaacgac tttctgcgca
    16321 aagttcccaa ggagcagacc cgcgccaata tcgcgaaaat catcgaaacc gtgcagaagg
    16381 aaaacatccc cgccgtcctc gtcggcgtgc cgcacatcac actgggtgcg ttgttcgggc
    16441 atttgagcga tcatccgctg tatgaggatt tgtccgagga atacggcatt ccgctgttcg
    16501 gcggcgcgtg ggcggaaatt ttgggcgata ataatctgaa atccgaccaa atccacgcca
    16561 acggcaaagg ctatcggaaa tttgccgaag atttgaatca atttttgaga aaacaggggt
    16621 ttagataaac aaaggtttat ccgcacccaa gttgtttata taatcatgaa ccgactggga
    16681 caccaaactg cttcgggacg catatgccgt ctgaagtgca aagcctacgc catacagccg
    16741 catgaagttg cagcggtatg gcgttttttg aaaaagacgg cctgccggtt cagacggcat
    16801 gaccgaccgt ccgagcctgc tgtggataaa gcccggacag gctgaaatca tggaatattg
    16861 cgaacctgaa gaagcatccg acccgtacgc aacatacagg cgtgccaacc tgatggcggg
    16921 tctgccgctg tttgtcgtga ttttggttct gctcaatatt gtttttccgc ttccggcgta
    16981 tccattggct tggctggtgc ttgcaggttt catggttttg gtcagcggct ttcccttatc
    17041 gctgctgctt gtggtgctgc ttgtcctgac ctgctgcatt ctagcgcgtt gtccgccatt
    17101 atcccgtctt ttgtgctacc cttgcccgaa tcatccgatg tctaaaaatt ctgcctgatg
    17161 gcagccctac aaacccgaag gagtagaaat gaaactgtcc gaactgttca accccaacga
    17221 atttgccgcg cgacatttga gtttcggcga cgaggccgcg cttttggaaa cgctcggcga
    17281 gaagagtatg gacgattttg tcggcaatac cgtgccgcaa agcatccgta tgccgtccga
    17341 actcgatttg cccgaggcct tgaccgaggc ggacgcattg gcgaaattga aaggcattgc
    17401 gtcgaaaaac gtaatcaaca agtcctatat cggtttaggc tattacccga cccgcgtgcc
    17461 gaacgtgatt ttgcgcaacg tgttggaaaa tccgggctgg tacaccgcct acacgccgta
    17521 tcaggcggaa attgcccagg gtcgtttgga agcgttgtta aacttccaac aagtgtgtat
    17581 cgatttgacc ggtttccctg tggcgggcgc gtctttgctg gacgaggcaa ctgctgcggc
    17641 tgaagcgatg gcgatggcgc accgcgtggg caaggtgaaa tccgagcgtt tctttgtgga
    17701 tgcgcgcgtg tatccgcaaa ctttggacgt gatgaaaacc cgcgccaaat atttcgactt
    17761 tgaactggtg gtcggcgatt ttgctcaagc cgacgagggc gaatacttcg gcgcgctgtt
    17821 ccaatacgtc ggcaaagacg gcgatgtgca agacttgcag gacgttatcg gccgtctgaa
    17881 agccaaaggc acaatcgtgg ccgtttccgc cgacattatg agcttggttt tgctgaaatc
    17941 gcctgccgaa ttgggcgcgg atattgcttt gggcaacacc caacgcttcg gcgtaccgat
    18001 gggcttcggc ggtccgcatg ctgcttattt cgcgtttaaa gacgagttca aacgttccgc
    18061 cccgggccgc atcatcggcg tatccaaaga cgcatcgggc aaacctgcct tgcgcatggc
    18121 gttgtccacc cgcgagcaac acatccgccg cgaaaaagcg acatccaata tttgtaccgc
    18181 gcaggcattg ctggcgaact tggcgggtat gtacgccgtt taccacggcc ctgaaggtgt
    18241 gaaacgcatc gccaaccgca ttcacgcgct ggcttctgcc tttgccgatg cgctggtttc
    18301 agacggcctg aatgtggttc ataaggtctt cttcgatacc gttaccgtcg atttcggcaa
    18361 caaagagaaa gcagaccaag tgtttgccgc tgctttggaa tcgggctata acctgcgccg
    18421 cgttaacgat actcaagttg cggctgcatt ccacgaaaca tcggcatgcg aagatttggt
    18481 tgatttgtac cgcgcgttta ccggcaagga tgcgttcgca tttgccgatg atgtcaaagg
    18541 ccgtctgaac gccgaattgc tgcgtcaaga tgacattctg caacatcctg tgttcaaccg
    18601 ttaccacacc gaacacgaaa tgctgcgcta cctgaaaaaa ctcgaagacc gcgatttggc
    18661 gatgaaccgc agcatgattt cgctcggtag ctgcaccatg aaactcaacg cgactgcgga
    18721 aatgctgccg attacttggg cggagttctc cgacatccac ccttacgctc ccgaagcgca
    18781 aaccgtcggc taccgcgaat tgcttgccga tatggaaaac agcctgaaag ccatcaccgg
    18841 ctttgacgca atttccttcc agcccaactc cggcgcgcag ggcgaataca gcggtatgct
    18901 tgccatccgc cgctatcagg aagcgcaagg cgaagcgcag cgcaacatct gcctgattcc
    18961 caaatccgcc cacggcacca accccgccac cgccgccatg ctcggtttga aagtcgtcgt
    19021 cgtcgatacc gacgaacacg gcaacgtcaa catcgacgat ttgaaagcca aagccgagca
    19081 acaccgcgac gctttgtccg ccatcatgat tacctatccg tccacccacg gcgtgtacga
    19141 agaaggcatc cgcgacatct gccgcatcat ccatgaaaac ggcggacagg tttatatgga
    19201 cggcgccaac ctcaacgccc aaatcggcat catgcagcct gccgaagtcg gcgcggacgt
    19261 gttgcacatg aacctgcaca aaaccttctg tatccctcac ggcggcggcg gtccgggcat
    19321 gggtccgatt ggcttgaaag cccatctggc tccgttcgca ccgggacacg ctttgaccga
    19381 cacccacagc gccagtgccg accaaaccgc cgttgctgct gcggccttcg gttctgcgtc
    19441 catactgccg attacttgga tgtatctgac catgatgggc aaacaaggca tggaacaggc
    19501 gacacgctgg gcattgctca acgccaacta cgttgccaaa cggctgggcg aagactatcc
    19561 gatcctgtat acaggcaaaa acggccgcgt cgcgcacgaa tgtatcgtcg atttgcgtcc
    19621 gctcaaagcc gaaagcggca ttaccgaaac cgacatcgcc aaacgcctga tggactacgg
    19681 cttccacgcc ccgacggttt ccttccccgt tgccggtacg ctgatgatcg agccgaccga
    19741 gagcgaaagc aaagccgaac tcgaccgctt catcgccgcc ctgaaacaaa tcaaacagga
    19801 agtgctgaaa gtcgggcgcg gcgaatggcc gaaagacgac aacccactgg tcaacgcgcc
    19861 gcacaccgcc gcagatataa ccggcaactg ggcgcatccg tattcccgcg aagaagccgt
    19921 cttcccgttg ccgttcgtcc gcgaacacaa gttttggccc ttcgtcaacc gcgtggacga
    19981 cgtgtacggc gaccgcaatc tcgtgtgcag ctgcccaccg atggaaaatt atgaagactg
    20041 actgttgata tcttaaaaaa tgccgtctga aacattttca gacggcattt tcatcaacgg
    20101 caaaccagtt gcaccaatac acgtatctcg actataactt taaaacaaat gagttaaacc
    20161 agtatccata catcagcttt tttatcatcc tactttttat tcatccgatc gtgcaaacag
    20221 atttcaaaga tgaaaagcct attcacaccc tttgatgtca tttccacacg gacaaa
//
LOCUS       DAWKQJ010000020        19791 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000020 DAWKQJ010000000
VERSION     DAWKQJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.020"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(209..284)
                     /locus_tag="WJL20_000546"
     tRNA            complement(209..284)
                     /locus_tag="WJL20_000546"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="WJL20_000547"
     CDS             complement(377..736)
                     /locus_tag="WJL20_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ7682730.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="WJL20_000548"
     CDS             complement(759..1403)
                     /locus_tag="WJL20_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ7682731.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="WJL20_000549"
     CDS             complement(1405..1941)
                     /locus_tag="WJL20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ7682732.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="WJL20_000550"
     CDS             complement(1953..3050)
                     /locus_tag="WJL20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7682733.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="WJL20_000551"
     CDS             complement(3053..4489)
                     /locus_tag="WJL20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ7682734.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WJL20_000552"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WJL20_000552"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ7682735.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5479..6126)
                     /locus_tag="WJL20_000553"
     CDS             complement(5479..6126)
                     /locus_tag="WJL20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ7682736.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WJL20_000554"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WJL20_000554"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ7682737.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7501..8577
                     /locus_tag="WJL20_000555"
     CDS             7501..8577
                     /locus_tag="WJL20_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ7682738.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8784..10250)
                     /locus_tag="WJL20_000556"
     CDS             complement(8784..10250)
                     /locus_tag="WJL20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ7682739.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WJL20_000557"
     CDS             complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WJL20_000557"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ7682740.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WJL20_000558"
     CDS             complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WJL20_000558"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ7682741.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVCPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13529..13813)
                     /locus_tag="WJL20_000559"
     CDS             complement(13529..13813)
                     /locus_tag="WJL20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7682742.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WJL20_000560"
     CDS             complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WJL20_000560"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ7682743.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15830..16531)
                     /locus_tag="WJL20_000561"
     CDS             complement(15830..16531)
                     /locus_tag="WJL20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ7682744.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16674..17816)
                     /locus_tag="WJL20_000562"
     CDS             complement(16674..17816)
                     /locus_tag="WJL20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ7682745.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGLMFNDLDGI"
     gene            complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WJL20_000563"
     CDS             complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WJL20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ7682746.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18542..19684
                     /locus_tag="WJL20_000564"
     CDS             18542..19684
                     /locus_tag="WJL20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7682747.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5104 a         5100 c         4588 g         4999 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctaggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccagaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt ttcgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccagg taagcagctt ctcactgctg
     1261 accatttcgt ccacatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa ggccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accctggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gtgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaaccc tcttctgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catccggaat gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg
     4861 ttacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta
     4921 ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggcga
     5101 taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccgca acccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgccat tagattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg
     5581 attggcaggc ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcgaatcaa
     5641 atctgcgcgc ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gtcaaacgga cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggcgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 ggattttaat cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc
     5941 caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cgttccccaa cctgcaaaat acaggattaa atgttcgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatcca ttgtcatatc
     6181 tgccgtcaaa gacaatcgta ttctggatgt accttttggt actgtcggtg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcaggta ttcagctttg gcaagggtgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcgc ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgagc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata
     6961 gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcggttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc
     7801 aaatccatac agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac
     7861 cctttggggc cggtttttgt ccgcttaggc gaccctaaac tcggtttggg catacacggg
     7921 accaatgcgc cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg
     7981 cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat
     8041 caaatggcgg gtctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac
     8101 ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca
     8161 aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc
     8221 ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg
     8281 ctggcgtgga tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag
     8341 gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg
     8401 cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa
     8461 ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggctgcttc cgagccggtt
     8521 gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata
     8581 accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt
     8641 tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca
     8701 tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat
     8761 tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg
     8821 ataacgaaaa aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct
     8881 cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg
     8941 agggtcatca taatgttaga tgcggccgcc atcatcagcg cggtcggcat cgccggggca
     9001 ttcagtgaaa cggcagcagc gaaaaatgcg ccgaacatag cggtaatatg tgcagtagta
     9061 ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg
     9121 ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt
     9181 ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc
     9241 cacgcgcttt tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg
     9301 cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata
     9361 agggcgggaa catctgccca caacagcaac aagataccga aaatgaccgc cataatgatt
     9421 tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg aacggcattg
     9481 ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag
     9541 gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta
     9601 ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata
     9661 aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc
     9721 ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc
     9781 ggatgtataa tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt
     9841 tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac
     9901 aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg
     9961 atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg
    10021 cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc
    10081 gcgcccaacg gcatggcctt gccgataatg gcggcaatca caccgacaaa catggccagc
    10141 agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac cagggcgcac
    10201 aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc
    10261 gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat
    10321 atattccgat atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat
    10381 ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc
    10441 cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac
    10501 cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac
    10561 cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc
    10621 cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag tctatgtcga
    10681 aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa
    10741 cactgtcttc attaacttgg ggggcggtca acacagtaaa aggcaatttt ttactgtgtt
    10801 cggtacgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg
    10861 tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt
    10921 caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacgggaggg
    10981 taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg
    11041 cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc
    11101 cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct
    11161 cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac
    11221 gccctgaaac cgccatatca taaggcacgc cggtaataac ccaatcggca tgactttcat
    11281 acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt
    11341 cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat
    11401 cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc
    11461 ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt
    11521 taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg
    11581 tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc
    11641 ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc
    11701 agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa
    11761 aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat
    11821 gccgtctccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa
    11881 cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg
    11941 atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc
    12001 ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa
    12061 caattcgccg acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc
    12121 caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc
    12181 gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc
    12241 ccacatactg tgcaacacac gcggcgcttc gggcgatggc gcatccagcc ggcgcggttt
    12301 gtaacgctca acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg
    12361 cccgctctcg gtgatgattg tcggatgcgg cagcccgtgt tcgagacaag cctgactgat
    12421 gccccatacg actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg
    12481 tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt
    12541 tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac
    12601 atcacggatg ttcccaagct gcgagcccaa atggaaatgc aaaagctgca ggcaatccag
    12661 cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa
    12721 ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc
    12781 gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat
    12841 ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc
    12901 gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc
    12961 ggcgtgtgcc aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg
    13021 tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac
    13081 caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat
    13141 gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg
    13201 atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acagtttgat tgtgttgcga
    13261 gggattgggg cagacgatga tttcgccgga atcgtcaaca tcataataac ctatgcccca
    13321 atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct
    13381 gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga
    13441 aaagcctgcc tcatacctga tgaggaacag gcaaaatgtc gtctgaacgc ttcagacggc
    13501 attttggttc atctttccat caaaggagtc agcgatcgag ctgctctttg atgattttca
    13561 ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca
    13621 aaacaggttt gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgtt
    13681 ccaacatttt tacggctttt gtgcggtttt gttcaaaatg gggatcaccg taaatctgac
    13741 gctcggcaga atcgtcggca aatgcggttg cggcactcag ggaaacaacg gcagccaata
    13801 acagtttttt cattttcagt cctttcttaa tcggttgatt gaataagatg tgtttttcaa
    13861 tgctgccatt aaaacacagc aaaattaggt ttgaattagc aggaagttaa gaagcgtaaa
    13921 tgccgtctga aaaaaacaga gccgtcaaac ggctccgttt tccttatgct tctttggttt
    13981 cagccacctt ttgacgcagg cgcaagctca attcgcgcaa ctgcttgtcg tccacgctgt
    14041 tgggcgcgtt ggtcagcagg cattgggcac gttgtgtttt cgggaaggca atcacgtcgc
    14101 ggatagattc ggcgccggtc atcagggtaa ccaaacggtc gaggccgaat gcaaggccgc
    14161 cgtgaggagg cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc tcttgttgct
    14221 cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgca cggtggatac
    14281 ggatagagcc gccgccgatt tcccagccgt tcaataccat atcgtaggcg cgcgccaaac
    14341 aatttgccgg gtcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat
    14401 ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aaatcgacaa
    14461 cccacaaagg tttccattcg tctgtgaagt agccgttgtc tttgccgtgt tccaagccga
    14521 ctttgatacg caatgcgccg atggcttcgt tcacaacttt ggttttgtcc gcaccgaaga
    14581 aaataatatc gccgttttgt gcgccggtac gctcgatgat ttctttcagg gcattctcag
    14641 acaggaattt tacgattgga gattgcaggc cgctgtcttc gccgtttgag aggttgccca
    14701 catcgtttac tttgatgtat gccagacctt tcgcgccgta gatgccgaca aatttagtgt
    14761 attcgtcgat ttctttgcgg ctgaattttg cgccgttcgg tacgcgcagg gcgactacac
    14821 ggccgccttt catatctgcc gcgccacgga agactttgaa ttcttccgtt ttcatcaggt
    14881 cggtcaactc ggtaaatttc aagttgatac gcatatccgg tttgtcagag ccgtagtaga
    14941 acatggcttc ggcgtaaggc atacgcggga agtcgcctaa atctacgttt aaagcatctt
    15001 tgaagacttg tttcgccata ccttcggtga tgtccataat ttcatcctcg ttcaagaacg
    15061 aggtttccaa gtcgatttga gtgaattcag gctgacggtc ggcacgcaag tcttcgtcac
    15121 ggaaacattt ggtaatttga tagtaacggt cgaaaccggc caccatcaac agttgcttaa
    15181 ataattgcgg tgattgcggc agcgcgaaaa actcgcccgg atgaacgcgg ctcggcacga
    15241 ggtagtcgcg cgcgccttcc ggagtggagc gggtcagcat cggcgtttcg atgtcgatga
    15301 agccttgcgc gtccagataa cggcgaacgc ccatagcgac ttgataacgc aggcgcaggt
    15361 tgcgttgcat caccggacgg cgcaagtcga taacgcggtt ggtcaggcga acgttttcgc
    15421 tgatgttttc atcgtcgatt tggaacggcg gcgtggcggc ggcgttcaag acttcgattt
    15481 ctttggcaag gatttcgatt ttgccggaaa tcattttgtc gttggtcgta ccttcgggac
    15541 ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgagcggaa tcggcagcgg
    15601 caaacgcttc gggcgtgtcg gggtcaatca cgacttggac gatgccttcg cggtcgcgca
    15661 ggtcgataaa aatcacaccg ccgtggtcgc gccgacggtg tacccagcct ttgacggtaa
    15721 cggtttggtc taagtattgc tcgctgatca ggccgcagta gttggtacgc ataaaatcac
    15781 cttttattga tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt
    15841 cgggcaaatc cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt
    15901 cagggatgac catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga
    15961 gttcgcgcac atcgcttttc tttaccataa tatagtaacc gccggtcggg ttgggcgtgg
    16021 tcgggacata cacggaaaga taatcgccat cctgcggcaa tgcggcctta accgcattcg
    16081 acacctgccc tgacacgaaa gcaatcgtcc aaatacccga ttggggaaac ggcacgagta
    16141 ccggcgtttt aaacgagcgg ctgctgtcgg acagcagcga ttcggatact tttttcacac
    16201 tcgagtagat ggatttgaca accggaatcc gccccaacag gctgtcccac gcggcaagaa
    16261 tctgccggcc caatacgttg gcggcaaata atccggttac aaacaatacg gcaatggcaa
    16321 caataacgcc cagccccggg atattgaagc ccaaaacata ttgcggccgc cattgcttcg
    16381 gcagcaggtt gacgagctga tcggacgcgg aaacgatata ggaaaccacc caaaccgtta
    16441 ccgcaatcgg cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag
    16501 ctttgccgcc ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa
    16561 accgcacatt atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac
    16621 cgccggcggc ttcagacggc acggcaactt gatatatcgt ctgaacacgc ggttcagatg
    16681 ccgtccaagt cgttgaacat caacccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cataaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atctgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaacc gcaccctttc cgccatttcg
    17881 ggattttgcc cccgcaatac agaaaacccc gaaaccgtcg ggcttcaggg ttttccgctt
    17941 atcgcgtatc aaccgccttg gcggttttgc aaaaatcaag ccagtgcttt tacttttgca
    18001 gacagacggc ttttgtggcg tgccgctttg tttttgtgga acacgccctt gtcggcgatg
    18061 cggtcgatga ctttgacaga ctcttggtaa accgcttgtg cggcagcttt atcgcctgct
    18121 tcgactgctt tcaatacttt tttcactgca gtgcggaatg cggtacgcag gctagcgttg
    18181 tgggcgcgtt gtttgacgga ctggcgggca cgtttgcgtg cttgtgcgct gtttgccata
    18241 ttggaatctc ctgaaagaaa aataaattct gtaaacgcgc aattttaaag ggggtttaca
    18301 ttatatgcaa gccttttctc tctatccgcc cgtcggacac cctgacagcg caaaaaaacg
    18361 gcaaaatcgt gcggacgttt tctccctctt ttgaaaacaa ggtaattttt taggaaaatc
    18421 aggtcaatat cgctttgagt ttgcacaaat ttactttaca atgcccaaac cttgcgccgg
    18481 ggtgtttatg gggcgcgggg ttttcttttt ttccaacctc tttcatcagg aatctgcccg
    18541 tatgaaaaaa tccgtattag ccgttttggc cgcgctgtct ctggcagcct gcggcggcag
    18601 cgaaaaaaac gccgtgcaac ctcaagcaga cgctgcgtct gctgccaatg ccgaagccgc
    18661 tgccaccgat actctgaaca tctacaactg gtcgaactat gttgacgaga gtacggtcga
    18721 agactttaaa aaagccaaca atctgaagct gacttacgac ttgtacgaaa acaatgaaac
    18781 gctggaagcc aaaatgctga ccggcaaatc cggttacgat ttggttgtgc cgggcatcgc
    18841 cttcctgccg cgccaaatcg aggcgggcgc gtatcaaaaa gtcaacaaag acctgattcc
    18901 caactataaa aacatcgatc ccgaactctt gaaaatgctg gaaaccgccg acccgggcaa
    18961 ccagtatgcc gtcccctatt tctccggcgt gaacacgatt gcgattacgg cgaaaggcaa
    19021 agagctttta ggcggcaagc tgcccgaaaa cggctgggat ttgctgttca aacctgaata
    19081 cacccacaaa ctgaaatcct gcggcatcgc cctgtgggac accccgagcg aaatgttccc
    19141 gattttgctg aactacttgg gcaaagaccc caaaggctcg aatcctgaag atttgaaggc
    19201 ggcggcggaa gtgttgaagt ctatccgtcc tgatgtcaaa cgtttcagcc cgtccatcat
    19261 cgacgagctg gcgcgcggcg acatctgcct ggcggcaggc aacggcggcg atttgaactt
    19321 ggcgaaagca cgttccgaag aagtgaaaaa caacgtcggc atcgaagtgc tgaatccgaa
    19381 aggcatgggc ttctggattg agtcttggct gattcccgcc gatgcgaaaa acgtcgccaa
    19441 tgcccacaaa tacatcaact acacgctcga ccccgaaatc gcggcgaaaa acggcatcgc
    19501 cgtaaccttc gcccccgcca gcaaaccggc gcgcgaaaaa atgcctgccg aactggtgaa
    19561 cacccgttcc atcttcccga acgagcagga tatgaaagac ggtttcgtga tgccgcaaat
    19621 gagtacggat gcgaaaaaac tgtctgtcag cctgtggcag aaaatcaaag tcggcaccaa
    19681 ctgatttgaa gcattaaaaa tgccgtccga acatcgttcg gacggcattt tatattggat
    19741 tgaaatagaa atatttatat agtggattaa caaaaatcag gacaaggcga c
//
LOCUS       DAWKQJ010000021        19674 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000021 DAWKQJ010000000
VERSION     DAWKQJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19674
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.021"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(367..921)
                     /locus_tag="WJL20_000565"
     CDS             complement(367..921)
                     /locus_tag="WJL20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682748.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1006..1506)
                     /locus_tag="WJL20_000566"
     CDS             complement(1006..1506)
                     /locus_tag="WJL20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682749.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1910..3106
                     /locus_tag="WJL20_000567"
     CDS             1910..3106
                     /locus_tag="WJL20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ7682750.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLNHVREQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            complement(3209..4453)
                     /gene="clpX"
                     /locus_tag="WJL20_000568"
     CDS             complement(3209..4453)
                     /gene="clpX"
                     /locus_tag="WJL20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ7682751.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4643..5014
                     /gene="rbfA"
                     /locus_tag="WJL20_000569"
     CDS             4643..5014
                     /gene="rbfA"
                     /locus_tag="WJL20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ7682752.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            5047..5970
                     /gene="truB"
                     /locus_tag="WJL20_000570"
     CDS             5047..5970
                     /gene="truB"
                     /locus_tag="WJL20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ7682753.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYT
                     EIGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            6187..8397
                     /locus_tag="WJL20_000571"
     CDS             6187..8397
                     /locus_tag="WJL20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ7682754.1"
                     /translation="MKTDIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNSQPASQPASQPASQPASQPASQP
                     ASQLYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQAVGEIKRHSDG
                     TPTENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSFGYNTNFKLSTW
                     LTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKG
                     NNDEYAFAGTHEYTLVFAKNKDKSTFYGFPIDEDNFLEKWEEDEIGFYKKGAPMRATG
                     TEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGY
                     NFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPK
                     TIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGT
                     TAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELA
                     PFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQ
                     KQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            8387..11164
                     /locus_tag="WJL20_000572"
     CDS             8387..11164
                     /locus_tag="WJL20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7682755.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFKKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNDR
                     TSNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYSAFLNWAENIQADNFSFLTTFWASLNDSDNANEQGKTRTEQALKFMQDNKF
                     GFAHLANWVKQNYQKHNIIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFTVD
                     RLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEGK
                     QPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLATF
                     KLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQETQF
                     TADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLDI
                     QNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGTK
                     EFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARLG
                     DLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFIE
                     PKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTESF
                     PLGAASLEKMK"
     gene            complement(11308..12480)
                     /locus_tag="WJL20_000573"
     CDS             complement(11308..12480)
                     /locus_tag="WJL20_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ7682756.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            12830..13276
                     /gene="iscR"
                     /locus_tag="WJL20_000574"
     CDS             12830..13276
                     /gene="iscR"
                     /locus_tag="WJL20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ7682757.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13305..14519
                     /locus_tag="WJL20_000575"
     CDS             13305..14519
                     /locus_tag="WJL20_000575"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ7682758.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            14783..15169
                     /gene="iscU"
                     /locus_tag="WJL20_000576"
     CDS             14783..15169
                     /gene="iscU"
                     /locus_tag="WJL20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ7682759.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15256..15576
                     /gene="iscA"
                     /locus_tag="WJL20_000577"
     CDS             15256..15576
                     /gene="iscA"
                     /locus_tag="WJL20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ7682760.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(15536..15757)
                     /locus_tag="WJL20_000578"
     CDS             complement(15536..15757)
                     /locus_tag="WJL20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ7682761.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            15839..16339
                     /gene="hscB"
                     /locus_tag="WJL20_000579"
     CDS             15839..16339
                     /gene="hscB"
                     /locus_tag="WJL20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ7682762.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16437..19187
                     /gene="gyrA"
                     /locus_tag="WJL20_000580"
     CDS             16437..19187
                     /gene="gyrA"
                     /locus_tag="WJL20_000580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ7682763.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19344..19674
                     /locus_tag="WJL20_000581"
                     /pseudo
     CDS             19344..19674
                     /locus_tag="WJL20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5551 a         4964 c         4593 g         4566 t
ORIGIN      
        1 cttttccgct ttggcaacga ttgaaaattt ccgtaaattc aaatatctag attccttcct
       61 gcacgggaat gacacggaag ggtttcagat gcagggtggg cattcctgcc cacccaatcc
      121 cgcccttgca acggtgggca agaatgctcg ccctatggtc tgacggttcg atatgatgcc
      181 gtctgaaaac ccaacggcgg catgacaatg ccgccctgcc aacgcacgta aatcagaatt
      241 gccatcccga catcaaacgc ttggaaacaa aatgccgtct aaaaatcaaa cggcaacata
      301 acaatgcccc taacaaatgc aaaaatgccg tctgaaagcc cttcagacgg cattggcacg
      361 ccggatttac cgccttctgc cgaaaccgcg catggcgggg cggcggtaat tggcgggcgg
      421 gcgcgttgtc gggcggtaac gctgcgcctg cgccgcctgt tgttttgcac ggaggctgcg
      481 cgtgttcaaa tccctgctgg tgcgcgcatt gggatgtgcg gactgatggt aggctgcacg
      541 cgcgcgccgg gcgacgggac tgtcttggtt gcctgcccgt gtgaatttgt ttgccagcgc
      601 gttgccgata aacgcgcctg ccgccgcgcc gactaggctt tgcagcagcc agcttcctgt
      661 cgattggtcg taaatatact gctgcccgtc tttaccggta acgggttgcc cgttgttgcc
      721 gtttgcctgt gcttcggcag gaatggtgtc tttgactgct tcgggagtca gttggtaaac
      781 cgtatcgtct gcctgctgtg cgagctgctg ttgcagggct tcaatctgtt tctgctgctg
      841 ttcgagccgc gcctgcgtgt cgtcttggca ggcggcgagt gcgaatgttg cgataagcgc
      901 ggaggcgatg atttttttca tgtgtgtcct gtttgggtgg aaaatcggtt ttattgtatc
      961 gccgtcggga attttggcaa gcattctgcc gacaaatcgt gatgtttaca ggggcagggt
     1021 gtgcagtttg cggacaaatg cgaggctgtt ggcgactggg ttgcctttgt tttcgacttc
     1081 gtgttcggtt tctacggtca gcaggcggcg gtttttgtgt ttgttcaggc tcaattcggc
     1141 ttgtgcgggt gtgaaaaaca ggcggtgttt ttcggcaaag cggcgggcgc ggctgttggc
     1201 ggttttcaaa atctgaagca gcgatacgtc cagatggaag cgtcgcacgc ccaatacgag
     1261 aatccgtgcg gcaaaaaaat cggcggcatc ggtaaactcg gcggggctgc tgcggctgaa
     1321 gtcgtgccgt tcggcggcta tcagggcggc aacagtgcgg atttgcggaa tcatcgattc
     1381 gctgataagt gtgtcgtccc gccctgcatc gagaagcatg ggggaaaaat cctgtgcatc
     1441 atcgacaata atgctacaag tgtgcagggt ttcgttttgt gcggcggttt ggggcattgc
     1501 attcatggtc attttcctga ttctgtcgtg tgttgccgaa tcgggcgacc tgtgtgaagg
     1561 taacaaaaaa gccgccccgt tttcgagcgg cctgttttgc gtatgggatg gatttcaagc
     1621 aagcgcaaaa aagtaccgca cgtctgtgtg gtaccaatag caataagcgg ttgtaaattt
     1681 tttgccttgc atgatgaaat gccgtctgaa gataaaaata ttggggagat tctaaatcaa
     1741 aacgctgccg cgcctcaagc attttatcga aatttttttg atttttcatc tatccgattg
     1801 aaaatatttc ggtttatttt taccgctgcc cgatattgtc ggcaatttcc ctttatctgc
     1861 tttgaaaaac ggtgcataat cccgagcaaa accgcaatca ggagcaatta tgcaaaacta
     1921 tctgaccccc aatttcgcct ttgccccgat gattcccgaa cgcgcttcag gcagccgcgt
     1981 ttgggatacg aaagggcgtg aatatattga tttttcaggc ggtatcgccg tcaatgcgct
     2041 gggacactgc caccctgccc ttgtcgatgc tttaaacgcg cagatgcaca agctgtggca
     2101 catttccaat atctatacga cgcgtccggc gcaggaattg gcgcaaaaat tggttgcaaa
     2161 cagttttgcc gacaaggttt ttttctgcaa ctcgggcgcg gaagcgaatg aggcggcgtt
     2221 gaagctggcg aggaaatacg cccgcgaccg tttcggcgga ggaaaaagcg aaatcgtcgc
     2281 ctgtatcaac agttttcacg gacgcacgct gtttaccgtg tccgtcggcg gtcagccgaa
     2341 atacagcaag gattatgcac ccctgccgca aggcattacg cacgttccgt tcaacgatat
     2401 tgccgcgctg gaagctgccg tcggcgaaca gacctgcgcg gtcatcatcg agccgataca
     2461 gggcgaaagc ggcatcctgc ccgccactgc ggaatatttg caaaccgcgc gccgtctgtg
     2521 cgaccggcac aatgcgttgt tgattttgga cgaagttcaa accgggatgg ggcatacggg
     2581 caggctgttt gcctatgaac attacggcat tgttcccgat attttgagtt cggcaaaagc
     2641 cttgggctgc ggctttccga tcggcgcgat gctggcgaca gaaaagattg ccgccgcctt
     2701 ccaaccgggc acgcacggat cgactttcgg cggcaacccg atggcgtgtg cggtcggcag
     2761 ccgcgccttc gacatcatca acgcgccgga aactttaaac catgtccgtg aacaggggca
     2821 gaaacttcag acggcattgc ggtctttggg agaacaaacg ggcgtgttta agcaagttcg
     2881 cggcatgggg ctgctgctcg gctgcgtgtt ggacgaagcc tatcgcggac gcgcatccga
     2941 aatcaccgcc gccgccttga aacacggcgt gatgattttg gttgcgggtg cggacgtatt
     3001 gcgtttcgcg ccttcgctac tgttgaacga tgaggatatg gcggaaggtt tgcgacgttt
     3061 ggaacacgcg ctgacggaat ttgccgcgac atcagacaat ccgtaaaact caaatgccgt
     3121 ctgaaggcgg gaaggcttca gacggcatca gaaacaaaaa accgcttcag aaacgtggtt
     3181 caacgttccg aagcggtttt gtttgccatc aggactcgaa caccaattcc ggttccctgc
     3241 cctcttcgat gactgcctta ccgaccacca cttttttcaa gcctttcaaa tcgggcaggc
     3301 ggtacatcgt atccaacagg cagcgttcga cgatggaacg caggccgcgc gcgccggttt
     3361 tgcgctccat tgcctgacgc gcgatggaac gcaatgcgcc ttcttcaaac tccagttcga
     3421 cattttccat accgaacaag gcttgatact gcttgaccaa agcatttttc ggctcggtca
     3481 aaatattaat cagcgcgtcc tcatccagtt cttctaacgt tgcaatcaca ggcaaacgtc
     3541 cgattaattc cggaatcaga ccgaatttga ttaaatcttc cggttcgacg atgccgaaca
     3601 gcttggtaat gtcggcattt tcgtccttgc tgtgaacgga cgcaccgaaa ccgataccgc
     3661 ctttctcggt gcgctggcgg atgacttttt ccaagcctgc aaacgcaccg ccgcagataa
     3721 acaggatgtt ggtggtatcg acgttgataa attcctgatt cggatgcttg cggccgcctt
     3781 ggggcggaac acttgccacc gtaccttcaa tcagtttcag caaggcttgt tgcacacctt
     3841 cgccggacac gtcgcgcgtg atggacggat tgtcgccttt gcgtgaaatt ttatcgattt
     3901 cgtcgatata gacgatgccg cgctgggctt tttcaacatc gaaatcacat ttgcccaaca
     3961 gtttggtgat gatttgctcg acatcttcgc cgacataacc tgcttcagtc agcgtggttg
     4021 catccgccat cacgaacggt acgtccagtt tacgcgccaa agattgcgcc agcagggttt
     4081 tacccgatcc ggtcgggccg ataagcagga tgttggattt cgacaattcg acattagctc
     4141 cggctttcgg gtggcgcagg cgtttgtaat ggttgtacac cgacaccgcc aaggctttct
     4201 tggcttgttc ctgaccgata acgtggtcgt tgaggttggc gacgatttcg gcgggcgtgg
     4261 gcagcttgcc ggattcttcc ggctcccctc cggcactttc cgaaggtgtg ccgtcatttc
     4321 cgtcttcatg caatatttca atacagtttg agacgcattc gtcacagata aaggcgtttt
     4381 cgccctcaat taaatgtttg acgtgtgatt tggattttcc gcaaaaggaa caggtacggt
     4441 tttcgttgga catagctttc tttacaatgt atgcgttaca gaaacggcac gcgccgttcg
     4501 ggttgccaag tataataact atatccgttc ttatcaatgt attaccttaa aatcccgccg
     4561 attaggctat aatacgccct ttcgcaaccg ccccggcggc aaaaatgccg tctgaaacca
     4621 aatctgaaat ctgaggatat tcatgagaaa accccaacgc ggctatgccc gccaagaccg
     4681 tgtcaaagaa caaattatgc gcgagcttgc cgaactcgtc cgtaccggac tgaaagaccc
     4741 gcgcgccggc ttcattaccg tcaacgaagt cgaagttacc cgcgattaca gccacgccac
     4801 cgtgttctac accattttaa accaagacgc gcgcgaaatt acggaagaag tgctggaaca
     4861 cgcgcgcgga cacctccgca gcgaattggc caaacgcatc aagctgttca aaatccccga
     4921 gctgcatttc aaatacgacg aatccttgga acgcggtatg aacctgtccg cccttatcga
     4981 ccaagtagcg gcggaaaaac cggttgaaga ctgacggata tgcccatgcc gtccgaacat
     5041 cgaaccatga atacaggcaa accccaaaaa cgtgccgtca acggtgtttt gctcttggac
     5101 aaacccgaag gcctttccag caacaccgcg ctgcaaaaag cgcggcgttt gttccgtgcc
     5161 gaaaaagccg gacataccgg cgtgctcgat cctttggcaa ccggactttt gcccgtctgc
     5221 ttcggcgaag cgaccaagtt ctcccaatac ctgctggatg ccgacaaagc ctacaccgcc
     5281 acgctgaaac tcggcgaagc cagcagcacg ggtgatgccg aaggcgaaat cgttgccacc
     5341 gcccgtgccg atatttcctt agccgaattt cagacggcct gcaaagcatt aacgggcgac
     5401 atccgccaag tgccgccaat gttttccgcg ctcaagcacg aaggcaaacc gctgtacgaa
     5461 tacgcccgca aaggcatcgt catcgaacgc aaagcgcgct acattaccgt ttacgccatc
     5521 gatattgccg aatttgacgc gcccaaagcc gtcatcgacg tacgttgcag caaaggcacc
     5581 tacatccgca ccctcagcga agacatcgcc aaacacatcg gcacgttcgc ccacctgacc
     5641 gccttgcgcc gcaccgaaac cgccggcttt accatcgccc aaagccacac gcttgaggcc
     5701 ttggcaaatt tgaacgaaac agaacgcgac agcttgctgc tatcctgcga cgtattggtt
     5761 tcacactttc cccaaaccgt tttaaacgat tatgccgtcc atatgctccg ctgcggacaa
     5821 cgtccgcgtt tcgaggaaga cctaccttcc ggcacgccgg tacgcgttta cactgaaatt
     5881 ggagaatttg tcggtttggc agaatatcaa aaggaaatat gccgtctgaa agccttgcgc
     5941 ctgatgaaca cggcggcatc agccgcctga acgacggtta aaaatacggg ctgtgtttga
     6001 ataatgtgtt gatatttccg caaaatcccg acacactcgg acaccctccc cgcttatcgc
     6061 aactttgcga acgccccccg aaacagaaaa gacatcaaag gattgatttt attagaattt
     6121 atttgcaaag ccatttgccg ttacacaaga atggcacatt aaaatgactg atgaggattt
     6181 ataacgatga agacagacat tcaaaccgaa ttaacccaag ccctgctatc acacgaaaaa
     6241 gtatgggcga acgaagaaaa aaccatttta gccaaaaaca ttctgttgga tttggtggaa
     6301 aaaaccgacc cgaccattat cggtttgtta ttgaataatg atgagttaaa acgccatttc
     6361 tttgtggaag tgaatggtgt tttggtgttt aaattgcagg atttacgttt tttcttggac
     6421 aaacacagcg tcaataattc ctacacaaaa tacgccaacc gcattggttt gacggacggc
     6481 aaccgctttt tgaaagacaa ttcggatatt gtgttggatt ttccgtttaa agattgcgta
     6541 ttgaatggcg gacaaagcac cgaagaaggc gaagagattt attttaaacg cagtaatagc
     6601 cagccagcca gccagccagc cagccagcca gccagccagc cagccagcca gccagccagc
     6661 cagccagcca gccaattata cactaaatta acccgaaaaa gacaagaaat cttttttaat
     6721 caaacgcttg cttttgatga aatcgatcgg ctttttgacg caaaagcatt ctcaaaattc
     6781 tctcgctata ccgcagacgg caaacaagcg gttggcgaaa tcaaacgtca ttcagacggc
     6841 acacccaccg aaaatctcat tatcaaaggc aataatctga tagccctaca ttcgcttgcc
     6901 aaacagttta aaggcaaagt gaagttgatt tatattgatc cgccttatta ttttgttaaa
     6961 aagaaacaac aagatagttt tggttataac actaacttta aattatctac ttggcttaca
     7021 tttatgaaaa atcgtttatc aattgccaaa gaattattaa ctgatgatgg gattattgtt
     7081 atttccattg atgatgacgg aaatgcttat ttaaagattt tgttagatga aatttttgga
     7141 tttgaaaatt ttatcggaaa tttacccact attatgaatt taaaaggtaa taatgatgaa
     7201 tatgcttttg caggcactca cgaatacaca ctagtctttg ctaaaaataa agataaatct
     7261 actttttatg gatttccaat agatgaagat aattttttag agaaatggga agaagatgaa
     7321 attgggtttt ataaaaaagg agctccaatg cgagcaacag gaaccgaaga taaaagagaa
     7381 gatagacctg aaatgttcta tccttttttg gtaaaaaata acacagttag tacaattaca
     7441 gatgaagaat tttctcaaat ctataataaa gatttagaag tattcaatga tgattttatt
     7501 caaaaactaa aagagaaata cgaaaatctt ggatacaatt ttattttacc tattgctgat
     7561 aaacaatggg ggcgttggcg ttgggggtat tcaataaaga ataaggcaag gctgcagacg
     7621 gatattattg ttagccaatc taaaaatgga atttccttgt ataaaaaaca aagacctgaa
     7681 ttagatgatt tacctacaaa aaaacctaaa actatttttt ataaacctga atacagtagt
     7741 ggtaatggaa ctgaacagat gaaaaatctt tttggtgaaa aggcatttaa aaatcctaaa
     7801 ccagaagaac ttatccaaga ttttattacc atcacaacca acgaatccga catcgtctta
     7861 gactaccatc ttggtagtgg cacaaccgcc gccgttgccc acaaaatgaa ccgacaatat
     7921 atcggtattg aacaaatgga ttatattgaa acgcttgccg ttgaacgctt gaaaaaagtg
     7981 attgatggcg agcaaggcgg tatttccaaa gccgtgaatt ggcaaggtgg tggcgagttt
     8041 gtttatgccg aacttgcccc atttaacgaa accgcaaaac aacaaatttt ggcttgcgaa
     8101 gattcagacg gcatcaaaac gctgtttgaa ggtttatgcg aacgctattt cttgaaatac
     8161 aacgtcagcg taaatgaatt tagtcaaatc attcaagagc ctgaatttca atccttggca
     8221 ttagacgaac aaaaacaaat ggtgcttgaa atgttggatt taaatcaaat gtatgtttca
     8281 ttatccgaaa tggatgatga gcaatttgca ggttgcttaa atgatgacga taaagcgtta
     8341 agccgtgaat tctatcaatc agcaaaaaat caagcggaga aaaaagatgg cgaataataa
     8401 aacgttgttt gaagtgattg aaaatgaacg taaagcggtt aaaaaataca agcctgaatt
     8461 acttgaaatg ccagaattta cgtccaaaaa cttaaaatat gatttttttg aatggcaaaa
     8521 atctgccctt gaaaactttt tgatttttga ccgcacttca aagctagacg atttccctga
     8581 tttaaaaaat aagccaacgc atttgctgtt caatatggca acaggggcgg gcaaaacaat
     8641 gatgatggcg gcgttgattt tgtattattt taaaaaaggg tatcggcatt ttttgttttt
     8701 tgtgaatcaa aataatatcg tggataaaac ggaaaataat tttattgacc cgacacacgc
     8761 aaaattttta tttaccgaga agattttgca gggcgatacg gtaattccca ttcgcaaagt
     8821 ggaaacattt agcccacatt cagacggcat tgaaattaaa tttaccagta ttcaaaaact
     8881 gtataacgat attcacaccg agcgagaaaa ccaaaccaca ttggcggatt tgcataaatt
     8941 gaaccttgtg atgctgggcg atgaagcaca ccatttaaac gcgcaaacca aaggcaaaaa
     9001 acaaggcgaa ttagatttag aaaaggaaat gaacgaccgc actagcaatg ccgaaattga
     9061 acgcaaaggc tgggagcata tggttttgga gttgttactc aataaaaatg gcaatcctag
     9121 ccaaaatgtg ctgttggaat ttaccgccac gctgcctgaa aatgctgacg tgcaacaaaa
     9181 atacgcggat aaaatcatca caaaatttgg cttaaaagag tttttgcaaa aaggttatac
     9241 caaagaaatc aatttggtat ccagtacgct gggtaagaaa gagcgggtgt tgcacgcttt
     9301 attatttgct tggtatcgcc atcaaatcgc gttgaaatat ggcattgcca atttcaagcc
     9361 tgtgatgttg tttagaagta agacgattga tgaatcaaaa gcggattatt cggcgttttt
     9421 aaattgggca gaaaatatac aggcggataa tttttcgttt ttaacgacat tttgggcaag
     9481 cttaaacgat agcgataacg ccaacgaaca aggtaaaacc cgcaccgaac aagccctaaa
     9541 atttatgcag gacaataaat ttgggtttgc acatttagca aattgggtaa aacaaaatta
     9601 tcaaaaacac aatatcatta ttaccaactc cgaaaccaac aaaaccaaaa ccgaaaaaac
     9661 cgacagcgaa acggaaaaat tgctgaataa tttggaagcg gctgataatc caatccgtgc
     9721 catttttacg gtagatagat taaccgaagg ttgggacgtt ctgaatttgt ttgatattgt
     9781 gcgtttgtat gaagggcaaa atggcggtgg ttcaaataaa aaatcaggca aaacggctgc
     9841 cgctaccgta tcagaaaaac agttgattgg tcgtggcgtg cgttattttc catttgcgtt
     9901 tgaaggtaaa cagccgaata aacgcaaatt tgacaacgac atgcaacacg aattgcgtat
     9961 cttggaagaa ttgttttatt acacgcacga tgagcaatcc cgctatattt cagaactgaa
    10021 aaatgagttg cgaaaagacg gttatttgcc tgaaaaagac gatgataagg tattggcaac
    10081 atttaaactc aaatctgaat ttgccgctaa taaggatttt agagaattgt taatttgggc
    10141 gaataaaaaa atccccaacc caaacgccaa aagcaataat gcagacagcc taaaagccaa
    10201 tccacaaacg ctttcattcc aagttcacgg taatcaactg ttgcaggaaa cgcaatttac
    10261 agccgatgaa aatgatgaaa ccgcccgaca aatcggcaca caaaataatt ttactcaaac
    10321 cataaaaatg agtgaaatgg agcggcacat tttcaataaa gcattacata tcaaagggaa
    10381 aaatagtcaa tctttattcc atttcaaccg tttgcaaagc aagctcgaca ttcaaaatcg
    10441 caatgaattg caaaataact tgttgaaaga ctggcaaatt gaatttttag gattagggca
    10501 agacaaacag gttcgcctgg acgataaact tgcaggctgc ctgaaaatct tggaaatggt
    10561 tgaaaaacat ctgaatgaaa gtgatatgcc gtttatcggt acaaaagaat ttacacctaa
    10621 aaaattatgg gaaattttcg gcacgccaaa acaaaaatgg gtcaaaaaag atgatgtaaa
    10681 aacgactatt gccacgcaaa atgattggta tgtgatgaat aattttgccg gaacgggttt
    10741 ggaagaagcg ttaattcaat ttatttcagc gcgtttgggc gatttgaaat ctcaatatga
    10801 cattcattta atccgtaatg aagaagtgtt taaattgaat aactttgccg atggtgaagg
    10861 atttatgccg gactttattt tattgctgaa agataaacaa aaatcttctt ccaatggcgt
    10921 gaaggacttt ttgcattacc aaattttcat tgagcccaaa ggtgagcatt tagtggaaac
    10981 tgatggatgg aaagaagaat ttttagaagc catcactgtg gaatatggga tggataagat
    11041 cctgcaaaaa gatacaccgc attatcgttt gatcggttta ccgtttttta ctgatcatca
    11101 gaaaaatgga caatttacgg agtcattccc tttaggtgcg gcatcgcttg aaaaaatgaa
    11161 gtagtgcatt gcaggcaacc ccgtttgaca aaacttcctt tacaaaaggg cgttttgtca
    11221 gatatttaat caacacatta ttaaaataca gccaaatttt aatgccgtcc gaaccctgtg
    11281 ttcagacggc atcgtatttt tcagtatcta aaccgtttcc ctgccccaat ctttgcctct
    11341 caaaatcgaa gcatcgacat cttgaatatc gcggtgtccc gtaaacgcca tagatatatc
    11401 catttcttta tacaggattt ccagcgcacg ggttacgcct tcttcaccat acgcacccaa
    11461 accatacaga aacgcccgac ctatcattgt acctttcgcg cccaaagccc acgccttcaa
    11521 aatatcctga ccgctgcgga tgccgctgtc catccaaact tcgatgtcgc tgcccactgc
    11581 gctgacgatg tcgggcaagg ctttgatggc agacacggta tcgtcgagct gtcggccgcc
    11641 gtggttggaa acaatcaatg cgtccgcgcc gcttttcgct gctttttccg cgtcttcggg
    11701 ttccataatg cctttgataa tcagcttgcc gccccacaaa tctttaatgc gcgccacatc
    11761 gtcccagctc aggcgcgggt cgaattgttc ggaagtccat gaagacagcg aagacaaatc
    11821 gccgacgttc ttcgcgtgtc cgacgatatt gcggaacgtg cggcgttccg tgttcagcat
    11881 tttcatgcac cattccggtt tggtcgccag attgattaaa ttggcgatgg tcggtttcgg
    11941 cggcgcggac aggccgtttt tgatgtcttt gtggcgttga cccaaaacct gcaaatcggc
    12001 ggtcaatacc aatgccgaac atttggcatc cttcgcgcgc ttaatcaggt tttccataaa
    12061 ctcgcggtcg cgcatcacat aaagctgaaa ccaaaatggt gcggaagtgt tctcggcaac
    12121 gtcttcaatc gagcagatag acatcgtgga caacgtaaac ggaatgccga acttctccgc
    12181 cgcccgcgcc gccaagattt caccgtcggc gtgtgccata ccggtaaaac ccgtcggcgc
    12241 aatcgccacc ggcattttca catcctgccc gatcattttg gtttccaggc ttcgaccttc
    12301 catattgacc aatacctttt gtcggaagcg gatatcttta aaatccgaag tgttttcgcg
    12361 gtaggtagtt tccgtccacg aacccgaatc gatgtaatcg taaaacatac gcggcatttt
    12421 gcgcttggca acgcggcgca agtcttcgat gcaggtcatt ttgctcaaat cacgtttcat
    12481 cttgtttttc cctgtttgac agcctgatgg aatatgccgt ccgaaaccgc ttgagccttc
    12541 agacagcatt tcaggcaata cccaaataaa tcactaagat ttagataatc tatatcaata
    12601 tcaaaaataa atcaaatcgt tttgaaaccg cttgccggca aaataccccg accaccgctc
    12661 ctcaaaaaac ataaccaact gatttaaaaa accaaacaaa taaagcaaaa tcagactata
    12721 ccgccattta aacaaacaat cggcaacagc tcccccttac ttgactgaaa cactcagata
    12781 ttggacaatt ctccccacta taaaaaagcc gacacgggca accaccacca tgagactgac
    12841 caccaaaggg cgttttgccg ttaccgctat gctggatttg gcgatgaacg cgcaaaccgg
    12901 cgccgtcaaa ctcagtgcca tcagcgaacg ccaaaacata tccctctcct atctcgagca
    12961 attgttcggc aaactccgcc gcgccggact ggttgaaagc ctgcgcgggc ccggcggcgg
    13021 ctacatcctc gccgcaccgg cggcacgcat caacatcgcc caaatcatcg ccgccgccga
    13081 agaccggctg gacgcgaccc aatgcggcag caaagccaac tgccaccacg gcgcgccctg
    13141 cctgacgcac gatctttggg agaatttaaa caaaaccatc aacgactacc tcggcagcgt
    13201 taccctgcaa agcatcatcg aacagaaaaa caacggcgac ggcagccgcg tcgtccaatt
    13261 tacacacatc cattaaataa cacccgaaaa agaaagagca aaccatgacc gtcaaaaccc
    13321 ccgtttacct cgactacgcc gccaccaccc ccgttgacaa acgcgttgcc gaaaaaatga
    13381 ttccctatct gaccgaaacc ttcggcaacc cagcctccaa cagccacagc ttcggctggg
    13441 aagcagaaga agctgtagaa aaagcacgtg cagacattgc cgccctgatt aacgccgact
    13501 ctaaagaaat cgttttcacc agcggcgcaa ccgagtccaa caacctcgct atcaaaggcg
    13561 cggcgcactt ctacaaatct aaaggtaatc acctcatcac tgtaaaaacc gaacacaaag
    13621 ccgtactcga caccatgcgc gaactcgaac gccaaggtta cgaagtaact tatctggacg
    13681 tacaagaaaa cggtttggtt gatttagacg tactgaaagc cgccatccgc gaagacacca
    13741 tcctcgtttc cgtaatgtgg gtaaacaacg aaatcggcgt ggttcaagat attcctgcca
    13801 tcggcgaaat ctgccgcgaa cgcaaaatca ttttccacgt tgacgcagca caagcatgcg
    13861 gcaaagtgcc tgtcgatgtc gaagccgcca aaatcgacct gctctcgatg tccgcgcaca
    13921 aagtgtacgg ccccaaaggc atcggcgcgc tgtacgtccg ccgcaaaccc cgcgtccgcc
    13981 tcgaagccca aatgcacggc ggcggccacg aacgcggctt ccgcagcggc acattgccga
    14041 cccaccaaat cgtcggtatg ggcgaagcct tccgcatcgc caaagaagaa ctcgaacaag
    14101 atatggcgca ctaccgcaaa ctgcgcgaca tcttcctcaa aggcatcgaa ggcatcgaag
    14161 aagtctatgt caacggcgac ctcgaacacc gcgccccgaa caacctgaac gtcagcttca
    14221 acttcgtcga aggcgaaagc ctgattatgg cggtgaaaga actcgccgta tccagcggct
    14281 ccgcctgtac ttctgccagt ctcgaaccca gctacgtcct gcgcgcgctc ggccgcaacg
    14341 atgaactggc gcactcatcc ctgcgcatca ccttcggtcg catgaccacc gaagaagaag
    14401 tgcaattcgc cgccgaactg attaaatcca aaatcggcaa actgcgcgaa ctgtcgccgc
    14461 tgtgggaaat gttcaaagac gggattgatt tgaactcgat tgaatgggca gcgcattaaa
    14521 gcgtaccaac atgccgtccg aacctttaga cggcattcca aaaacaaagc aatcaagaga
    14581 aaatatgaac gaacaagatt tagatttgga caatctcgac aacctgcttg aagattttga
    14641 cggcgttacc gtggaaggcg gcgtcgattc ggaaaacgac gacggctgcg aaggcggggc
    14701 gtgcaaaatc taaacttcag gccgtctgaa aatcggcaaa caaaccactt aaaccatcaa
    14761 aaaacattaa ggaaaccaca tcatggcata cagcgataaa gtaatcgacc actatgaaaa
    14821 tccgcgcaac gtcggcacat tcgacaaggg agacgattcc gtcggcaccg gcatggtcgg
    14881 cgcgcccgcc tgcggcgacg tcatgcgcct gcaaatcaaa gtgaacgacg agggcatcat
    14941 cgaagatgcg aaatttaaaa cttacggctg cggctcggcc atcgcttcgt ccagcctgat
    15001 taccgagtgg gttaaaggca aaagcctgga tgacgcgctg gcaatcaaaa acagcgaaat
    15061 cgccgaggag ttggaattgc cgccggtaaa aatccactgc tccatcttgg ctgaagatgc
    15121 ggtaaaagcg gccgttgccg actaccgcaa acgtcaggaa aacagataaa gcccttcaga
    15181 cggcatcatc ccgcaatgcc gtccgaacca cccgccgctt caggtccgtc cggggcggta
    15241 caacaaggaa gaaatatgat tacccttacc gagaatgccg caaaacacat caatgactat
    15301 ctcgccaaac gcggcaaagg cttgggcgta cgcttgggtg tgaaaaccag cggctgctcg
    15361 gggatggcgt acaaccttga atttgtcgac gaagccgatg gcgacgacct gattttcgaa
    15421 ggacacggcg cgcgcattta tatcgatccg aaaagcctgg tttatctgga tggcacgcaa
    15481 gtcgattaca ccaaagaagg tttgcaggaa ggtttcaaat ttgaaaaccc caatgtcaaa
    15541 gactcctgcg gctgcggcga aagcttccac gtttaaggca taaaaacggc gggaccgtat
    15601 caaaaccgtc ccgccatttt ttcgcttcct gcctgttgta gctgcctttg cctttccttt
    15661 tccgttccac cttgtgccgg aacaaatcgg atttcactaa ggcttttaaa gcattgtcgc
    15721 gtattttgcc tttattgtgc tgcactttgc cgcccatatt cagtcctttc gtttaagaag
    15781 cggcagatta taaggcaaaa acagttttct gccaaaatct tacatttatc atcctactat
    15841 gtcccaatat ttcaccctct tccggattga acccgctttc gatatcgaca ccgaaaactt
    15901 ggaacaaacc taccgcgcct tggccgcccg tttccatccc gataaattcg cttcagcttc
    15961 cgcctttgag caaaagcagg cagtgatgat gtcttccacc atcaacgatg cctaccgcac
    16021 cttgaaaaac cccatcgacc gcgccgccta cctgctgaaa acatcgggca tcgatgccga
    16081 cgcgccggag cataccgctt tcgcccccga attccttatg cagcaaatgg aatggcgcga
    16141 aacgctgatg gaggcacggg caggcaacga ccttgaatcc ttgaaaaatc tcgacaacga
    16201 aatccgcgac gaacaagaaa aactgttctg cggtctgaaa cagtcgtttg cccgacaaga
    16261 ttacgacaca gccgcacaac aagtccgcca aggcaggttt ctcgacaaac tccgcaacga
    16321 aatttcctcg gcattataat ccgcaccgtg tttcagacgg cgtaaccgcc gcaccgttcc
    16381 gcgtcaaaat atgctaaaat aagcaacaat tttttgccat acgaaacatt gaaaccatga
    16441 ccgacgcaac catccgccac gaccacaaat tcgccctcga aaccctgccg gtaagccttg
    16501 aagacgaaat gcgcaaaagc tatctcgact acgccatgag cgtcattgtc gggcgtgcgc
    16561 tgccggacgt tcgcgacggt ctcaagccgg tacaccgccg cgtactgtac gcgatgcacg
    16621 aattgaaaaa caactggaat gccgcctaca aaaaatcggc gcgcatcgtc ggcgacgtca
    16681 tcggtaaata ccacccccac ggcgataccg ccgtatacga caccatcgtc cgtatggcgc
    16741 aaaatttcgc tatgcgttat gtgctgatag acggacaggg caacttcgga tcggtggacg
    16801 ggcttgccgc cgcagccatg cgctacaccg aaatccgcat ggcgaaaatt tcccacgaaa
    16861 tgctggcaga cattgaggaa gaaaccgtca atttcggccc gaactacgac ggtagcgaac
    16921 acgagccgct tgtactgccg acccgtttcc ccacactgct cgtcaacggc tcgtccggca
    16981 tcgccgtcgg catggcgacc aatatcccgc cgcacaacct ttccgatacc gtcaatgcct
    17041 gcctgcgcct gctcgatgca cccgacaccg aaatcgacga actgatcgac attatccaag
    17101 cccccgactt cccgaccggg gcaaccatct acggcttgag cggcgtgcgc gaaggctata
    17161 aaacaggccg cggccgcgtc gttatgcgcg gtaagaccca tatcgaaccc ataggcagaa
    17221 acggcgaacg cgaagccatc gttatcgacg aaatccccta tcaggtcaac aaagccaagc
    17281 tggtcgagaa aatcggcgat ttggttcggg aaaaaacact ggaaggcatt tccgagctcc
    17341 gcgacgaatc cgacaaatcc ggtatgcgcg tcgttatcga gctgaaacgc aacgaaaatg
    17401 ccgaagtcgt cttaaaccaa ctctacaaac tgactccgct gcaagacagt ttcggcatca
    17461 atatggtggt tttggtcgac ggacaaccgc gcctgttgaa cctgaaacag attctctccg
    17521 aattcctgcg ccaccgccgc gaagtcgtta cccgacgtac gcttttccgg ctgaagaagg
    17581 cacgccatga agggcatatc gccgaaggca aagcggtcgc actgtccaat atcgatgaaa
    17641 tcatcaagct catcaaagaa tcgcccaacg cagccgaggc caaagacaaa ctgcttgcgc
    17701 gtccttggcg cagcagcctc gttgaagaaa tgctgacgcg ttccggtctg gatttggaaa
    17761 tgatgcgtcc ggaaggattg gctgcaaaca tcggcttgaa agagcaaggt tattacctga
    17821 gcgagattca ggcagatgct attttacgca tgagcctgcg aaacctgacc ggcctcgatc
    17881 aagaagaaat tgtcgaaagc tacaaaaacc tgatgggtaa aatcatcgac tttgtggata
    17941 tcctctccaa acccgaacgc attacccaaa tcatccgcga cgaactggaa gaaatcaaaa
    18001 ccaactatgg cgacgaacgc cgcagcgaaa tcaacccgtt cggcggcgac attgccgatg
    18061 aagacctgat tccgcaacgc gaaatggtcg ttaccctgac acatggcggc tatatcaaaa
    18121 cccagccgac caccgactat caggcgcagc gtcgcggcgg gcgcggcaaa caggcggctg
    18181 ccaccaaaga cgaagacttt atcgaaaccc tgtttgttgc caacacgcat gattatttga
    18241 tgtgctttac caatttgggc aagtgtcatt ggattaaggt ttacaaactg cccgaaggcg
    18301 gacgcaacag ccgcggccgt ccgattaaca acgtcatcca gttggaagaa ggcgaaaaag
    18361 tcagcgcgat tctggcagta cgcgagttcc ccgaagacca atacgtcttc ttcgccaccg
    18421 cgcagggaat ggtgaaaaaa gtccaacttt ccgcctttaa aaacgtccgc gcccaaggca
    18481 ttaaagccat cgcgctcaaa gaaggcgact acctcgtcgg cgctgcgcaa acaggcggtg
    18541 cggacgacat catgctgttc tccaacttag gtaaagccat ccgcttcaac gaatactggg
    18601 aaaaatccgg caacgacgaa gcggaagatg ccgacatcga aaccgaaatt tcagacggca
    18661 tcgaagatga aaccgccgac agcgaaaacg cactgccgag cggcaaacac ggtgttcgcc
    18721 cgtccggtcg cggcagcggc ggtttgcgcg gtatgcgcct gcctgccgac ggcaaaatcg
    18781 tcagcctgat taccttcgcc cctgaaaccg aagaaagcgg tttgcaagtt ttaaccgcca
    18841 ccgccaacgg atacggaaaa cgcaccccga ttgccgatta cagccgcaaa aacaaaggcg
    18901 ggcaaggcaa tattgccatt aacaccggcg agcgaaacgg cgatttggtc gccgcaacct
    18961 tggtcggcga aaccgacgat ttgatgctga ttaccagcgg cggcgtactt atccgcacca
    19021 aagtcgaaca aatccgcgaa accggccgcg ccgcagcagg cgtgaaactg attaacttgg
    19081 acgaaggcga aaccttggta tcgctggaac gtgttgccga agacgaatcc gaactctccg
    19141 acgcttctgt aatttccaat gtaaccgaac cggaagtcga gaactgaaaa tcatctcccg
    19201 atgccgtctg aagattcaga cggcatttat tttatccctc atccgtcatc cagcttctca
    19261 caatatagcg gattatagtc aattaaaaac aaggggctgt cctagataac tagggaaatt
    19321 caaattaagt tagagttgcc cctatgagaa aaagtcgtct aagccggtat aaacaaataa
    19381 actcattgaa ctgtttgtcg caggtgtaac tgcaagaacg acagcagagt tagtaggcgt
    19441 taataaaagt accgcagcct attattttca tcgtttacga ttacttattt atcaaaacag
    19501 tccgcatttg gaaatgtttg atggcgaagt agaagcagat gaaagttatt ttgctgaacg
    19561 acaaaaccat attaatggaa ttgagaactt ttggaaccgg gcaaaacgtc atttacgcaa
    19621 gtttgacggc attcccaaag cgcattttga gctgtattta aaggggtacg aacg
//
LOCUS       DAWKQJ010000022        19572 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000022 DAWKQJ010000000
VERSION     DAWKQJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.022"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(354..629)
                     /locus_tag="WJL20_000582"
     CDS             complement(354..629)
                     /locus_tag="WJL20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7682764.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(626..1909)
                     /locus_tag="WJL20_000583"
     CDS             complement(626..1909)
                     /locus_tag="WJL20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7682765.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2065..3483)
                     /gene="glnA"
                     /locus_tag="WJL20_000584"
     CDS             complement(2065..3483)
                     /gene="glnA"
                     /locus_tag="WJL20_000584"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ7682766.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3567..3716
                     /locus_tag="WJL20_000585"
     CDS             3567..3716
                     /locus_tag="WJL20_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682767.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            complement(3757..4143)
                     /locus_tag="WJL20_000586"
     CDS             complement(3757..4143)
                     /locus_tag="WJL20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682768.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVQVGIDSDNNTTFGDADSRTD
                     NRAWFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPS
                     YAEWNYVIPGTLGEAQYKFVCNGYPR"
     gene            4314..5123
                     /gene="aroE"
                     /locus_tag="WJL20_000587"
     CDS             4314..5123
                     /gene="aroE"
                     /locus_tag="WJL20_000587"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ7682769.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELSRLFGIEAVPMADLNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            5125..5826
                     /gene="mtgA"
                     /locus_tag="WJL20_000588"
     CDS             5125..5826
                     /gene="mtgA"
                     /locus_tag="WJL20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ7682770.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6151..6226)
                     /locus_tag="WJL20_000589"
     tRNA            complement(6151..6226)
                     /locus_tag="WJL20_000589"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6191..6193),aa:Phe,seq:gaa)
     gene            complement(6417..7151)
                     /gene="lptB"
                     /locus_tag="WJL20_000590"
     CDS             complement(6417..7151)
                     /gene="lptB"
                     /locus_tag="WJL20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ7682771.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7225..7755)
                     /gene="lptA"
                     /locus_tag="WJL20_000591"
     CDS             complement(7225..7755)
                     /gene="lptA"
                     /locus_tag="WJL20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ7682772.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7736..8317)
                     /gene="lptC"
                     /locus_tag="WJL20_000592"
     CDS             complement(7736..8317)
                     /gene="lptC"
                     /locus_tag="WJL20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ7682773.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8314..8850)
                     /locus_tag="WJL20_000593"
     CDS             complement(8314..8850)
                     /locus_tag="WJL20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7682774.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8915..9889)
                     /locus_tag="WJL20_000594"
     CDS             complement(8915..9889)
                     /locus_tag="WJL20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ7682775.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9985..11040
                     /gene="tal"
                     /locus_tag="WJL20_000595"
     CDS             9985..11040
                     /gene="tal"
                     /locus_tag="WJL20_000595"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ7682776.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRASCGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALEAQVANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11287..11646
                     /locus_tag="WJL20_000596"
     CDS             11287..11646
                     /locus_tag="WJL20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7682777.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTNDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            11720..11917
                     /locus_tag="WJL20_000597"
     CDS             11720..11917
                     /locus_tag="WJL20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682778.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            11934..12821
                     /gene="gluQRS"
                     /locus_tag="WJL20_000598"
     CDS             11934..12821
                     /gene="gluQRS"
                     /locus_tag="WJL20_000598"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ7682779.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRYRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(12891..13901)
                     /gene="dusA"
                     /locus_tag="WJL20_000599"
     CDS             complement(12891..13901)
                     /gene="dusA"
                     /locus_tag="WJL20_000599"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ7682780.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(13932..14423)
                     /locus_tag="WJL20_000600"
     CDS             complement(13932..14423)
                     /locus_tag="WJL20_000600"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ7682781.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(14496..15254)
                     /locus_tag="WJL20_000601"
     CDS             complement(14496..15254)
                     /locus_tag="WJL20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7682782.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(15325..16191)
                     /locus_tag="WJL20_000602"
     CDS             complement(15325..16191)
                     /locus_tag="WJL20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7682783.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(16240..16518)
                     /locus_tag="WJL20_000603"
     CDS             complement(16240..16518)
                     /locus_tag="WJL20_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7682784.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(16518..16808)
                     /locus_tag="WJL20_000604"
     CDS             complement(16518..16808)
                     /locus_tag="WJL20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ7682785.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16811..17341)
                     /locus_tag="WJL20_000605"
     CDS             complement(16811..17341)
                     /locus_tag="WJL20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ7682786.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17649..>19572
                     /locus_tag="WJL20_000606"
     CDS             17649..>19572
                     /locus_tag="WJL20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ7682787.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPGLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSE"
BASE COUNT         4660 a         5497 c         4919 g         4496 t
ORIGIN      
        1 gttgacatat tgtgtgatat cgagggctgt accgtatttt cccatcgtat tttcccatcg
       61 tattttccca tcgtattttc ccgttcttta tatgagcggc aggcatcctc ataaaagaag
      121 ggataccgat aaggaaaaat accccagcat aaaaaatata ctgattaatc aatacaaatc
      181 caacaaacag ttcatcgtat tcttttctgt tggcgtataa aaatactgat atattccttc
      241 actttcatag ggcgacgctt tatgtaccgt cctgtgtgtt gaaacgtcgg ttttccttac
      301 cgcctgcccc gcttttccct gattgctgcc aactgccgtc cgaacttgaa ccgtcaggtt
      361 tcagacggca ttttttctgc ctgctcccac aattgccgtt tttgtgcggc ggtcagtttt
      421 ttgacggcga tttcctgcct gagtgccgtg cctttatcca tcccgcctgc aacgatacgc
      481 atcgccaccg gtttgaatac gcgggtatat ttcgcgcctt taccggttgt gtgggcggca
      541 agccgctgtt gcggattcgg gctgatgccg caatagaacg cgctgttttc acacaatatc
      601 aggtaaacgc tccagtttga cgcgttcatc tgcctgcatc ctgagttttc tccccgttcc
      661 aaggcgcaac tttcagcagc agcagcatac ccggtagggc gagtgcgaaa cacagttgga
      721 aaaacggtac ataaccgagc cattcaatca gataaccggc aaaggaattg atgaccgtgc
      781 gcgggacggc ggacaggctg gtaaacagcg caagctgcgt tgcggtaaat gcgggattgg
      841 tctcgcgcgc catatacgat acgaacgccg ccgtccccaa acccacgccg actgcctctg
      901 cgccgataac tgccgccagc atcagcctct cgcctgtgcc gaccgtgtcg aaatgtccga
      961 accctgccag ccatacaaac cccaaaacgg ttacagcctg caccgcgcca aatagccaca
     1021 aggctttgtt tacgccgatt ttcagcatcc acacgccgcc caagatacct gccgccactg
     1081 ccggccacag tcctgcattt ttcgcaatca aaccgatgtc tgtcttgctg aaacccatat
     1141 ccagataaaa cggcgttgcc aacgcggttg ccatactgtc gccgagtttg taaaggaaga
     1201 taaacagcag cacgcacacc gccgaagcga tgcccttgcg cgtaaaaaat tctttaaacg
     1261 gctctaccac ggtctgcttc aacgttttag gaacggcagg cggcaacacg ggttcgcgcg
     1321 caagaaacag cgtcatcaga aggccgggca gcataaataa tgaagtgata acaaatactt
     1381 ctgaccacgg catcctgtct gccaacacca aactcaatga accgggaatc agggcggcaa
     1441 tccggtaggc gttcacatga accgagttgc ccaaacccaa ttcttcgtct gacaaaatct
     1501 cgcgcctgaa cgcatccaat acaatatcct gactggcgga aaaaaaagcg acaagcaccg
     1561 acaagccggc aatcagcggc agatgattac gggggtttaa aaaggcatag gccgccaaag
     1621 ccgccagcaa ccctgcctgc gtcagcagca tccacccgcg ccgccgtccc aaaacgggca
     1681 gcctgaccgc gtccatcagc ggcgaccaca aaaatttcca agtaaacggc agaccgatta
     1741 acgccatcag cccgatgctc ttcaaatcca cctgctcgct gcgcaaccac gccggaatca
     1801 ggttaatcag aaagtacagc ggcagccccg aggtaaaccc cgtaaaaata cagacaagca
     1861 tatttcggga aaagatttgc ccgataaaac ctgatttcga tgcagtcatc gttcgtgcag
     1921 cccaaaaaaa acaaaagcct attgtagcaa atatcgcccg cctgctgaaa aatgccgccc
     1981 cgcgccgccg gtttgccgtt caaatgcaaa atgccgtctg aaactgtgtt cagacggcat
     2041 tgcttttctg aaaaccagac gtgcttacag gctgtaatac atttcaaatt ccagcgggtg
     2101 cggcgccatg cggatgcggc gtacgtcttc ttctttgaaa gcgatgtagc tgtcgatcca
     2161 gtctttgctg aacacgccgc cgcgcaggag gaattcgtgg tcggctttga gggcggccag
     2221 tgcttcttcc aaagaagcgc aaacggtcgg caccaatgca tcttcttccg gcggcagatc
     2281 gtacaggttt ttatcggcag ggtcgcccgg atggattttg ttttgaatgc cgtccaaacc
     2341 cgccatcaac agggcggcaa atgccaaata cgggttggcg gtcggatcgg ggaaacgcgc
     2401 ttcgatgcgg cgcgccttgc tgctgttcac ggacggaatg cggatggaag cggaacggtt
     2461 tttggcggag tatgccagtt tggtcggcgc ttcaaagtgc ggcacgaggc gtttgtagga
     2521 gttggtggac ggattggtaa tcgcgttcaa ggctttggcg tgtttgatga tgccgccgat
     2581 gtagtagagc gcggtgtcgc tcaagccggc atagccgtcg cctgcgaaca ggttttgacc
     2641 gtctttccaa atggattggt gaacgtgcat accgctgccg ttgtcgccca taatcggttt
     2701 gggcataaag gtggctgttt tgccgaagtt gtgggcaacg ttttgaatca catatttcat
     2761 gtcttgggtt tggtcggcgc gtttgaccaa ggtggcgaaa cgcgtgccga tttccatttg
     2821 gctgccggta ccgacttcac tgtggtggac ttcgacttcg atgccgagtt cttccaaaat
     2881 gtttaccatc gcggagcgca ggtcttgtcc cgcgtcaatc ggagcaacgg gcgcgtaacc
     2941 gcctttgacg gcagggcggt ggccggtgtt ttgaccgtcc atatgcagac cgcttgccca
     3001 cgcgccgctt tcggacgtga tttcgtaacg ggttttgtgc atatcggttt caaattctat
     3061 gccgtcgaat acgaaaaact cgggttcggg accgaaatag gcggtgtcgc cgatgccgga
     3121 agatttcaaa taggcttcgg ctcggcgggc gatggagcgc gggtcgcggt cgtaaccctg
     3181 accgtcggcg ggatcgataa cgtcgcaagt caacacaaca gtcgcatcat cataaaaagg
     3241 atcgacgaag gctgtagacg catcggggcg caactgcata tcggaagcct gaatgccttt
     3301 ccagccgccg atagacgaac cgtcaaacgc ctgaccgttt tcaaaccact cttcggggtc
     3361 ttccaacacg atgcgcgcag gcacggtaaa gtggtgctgc ttgcctttgg tatcggtaaa
     3421 gcgcaaatcg acaaagcggg cttcgctttc ttcaatcaat tttacggcgt ttttaataga
     3481 catttttcag ctcctggaaa agggaaatag caaaatatgc ggaacgcggc ggcttgacgc
     3541 aaagtcggca tgattctacc acgtccatgc cgtctgaaac tcgccctgcg tcaaatttca
     3601 agctggaaaa tatagtttca gacggcaaga ttgtcaacaa tatgcttctg tttgtctgtt
     3661 caaaagcgga atacccgccc ttttgtccga atgccgacaa aacagatgcc gtctgaaagc
     3721 agttcagacg gcatctgttg tttggtttga aaacggtcag cgaggatagc cgttgcagac
     3781 aaatttgtac tgagcctctc caagcgtacc gggaataaca taattccatt cggcatagga
     3841 tggtgtataa gaagcgatag agtttcccga tttggaatac cacgttgccg tcaatatcct
     3901 aaaacgttta ccactgcaat ccatttcttc caacgtttta gctgtattaa aaaattttcc
     3961 attgccatac ttttgcggct tggcgaacct tgtttcaaac catgctctgt tgtccgtacg
     4021 gctgtctgca tcaccaaacg tggtattatt gtcactgtca atacctacct gcacccaatc
     4081 cgccgccacc gccggtgttg ccaaccccaa tgcaaatact gccaagcaca acatcttttt
     4141 catgtccgac cctctcgtcc ttgtcaacaa aataaagtta aattgtaaaa aaaaacgact
     4201 tttttcaaga gaaaaatatt ccgtcatgct atctgacaac cgtttcagac ggcatcgttt
     4261 ccatctgtac gccgccgtta taaaatagcc ttcccgtctt ttggaacacg accatgaccg
     4321 ccctcccccg ttactccgtt ttcggcaacc ccgttgcaca cagtaaatcg ccgcaaatcc
     4381 atcagcaatt tgcccttcag gaaggcgttg acattgaata cgaacgcatt tgcgccgaca
     4441 tcggcggttt tgcacaggcg gtttcgacat tttttgaaac aggcggttgc ggggcaaacg
     4501 ttaccgtacc gttcaaacag gaagcgtttc atctggcgga cgagcattcc gaacgcgcat
     4561 tggcggcagg tgcggtcaat acgctgattc cgttgaaaaa cggcaagctg cgtggcgaca
     4621 acaccgacgg tttgggtttg gtcggcgata tagtgaaggt gcaacacaca gaaattgaag
     4681 gcaaaaatat cctattgctc ggcgcgggcg gcgcggtgcg cggcgtgatt ccggttttga
     4741 aagaacaccg tcctgcccgt atcgtcattg ccaaccgtac ccgcgccaaa gccgaggaat
     4801 tgtcgcggct tttcggcatt gaagccgtcc cgatggcgga tttgaacggc ggttttgata
     4861 tcatcatcaa cggcacgtcg ggcggtctaa acggtcagat tcccgatatt ccgcccgata
     4921 tttttcaaaa ctgcgcgctt gcctacgata tggtgtacgg ctgcgcggca aaaccgtttt
     4981 tagattttgc acgacaatcg ggtgcgaaaa aaactgccga cggactgggt atgctagtcg
     5041 gtcaagcggc ggcttcctac gccctctggc gcggatttac gcccgatatc cgccctgtta
     5101 tcgaatacat gaaagccatg taatatgttc cgcatcgtca aatggctgat tgccctgccc
     5161 gtcggcatct ttatcttttt caatgcctat gtgtacggca acatcattac ctaccgcgcc
     5221 gtcgcgcccc atcggactgc ctttatgtcg atgcggatga agcagtttga acaggaaggt
     5281 cgcgatgtcg cactggatta ccgctggatg ccctacaaac gcatttccac caacctgaaa
     5341 aaagccctga ttgcttccga agatgcccgt ttcgccgggc acggcggctt cgattggggc
     5401 ggcattcaaa acgccatcag gcgcaaccgg aacagcggca aagtgaaggc gggcggctcg
     5461 accatcagcc agcagcttgc caaaaacctg tttttaaacg aaagccgcag ctatatccgc
     5521 aaaggcgaag aagcggcgat taccgcgatg atggaagccg ttaccgacaa agacaggatt
     5581 tttgaactgt atttaaactc aatcgaatgg cactacggcg ttttcggcgc ggaagccgcg
     5641 tcccggtatt tttatcaaat acccgccgcc aagctgacca aacagcaggc ggcaaaactg
     5701 acggcacgcg tccccgcccc gctctactac gccgaccatc cgaaaagcaa acggctgcgc
     5761 aacaaaacca atatcgtgct cagacgcatg ggttcggcag agttgcctga aagcgacacg
     5821 gactgattgt ttcagatatg gaaatgccgt ctgaactgcg gttcggacgg catatgtttt
     5881 ctggttttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
     5941 aatagcacgg aatcgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
     6001 taaggcgagc caacgctgta ccggtttaaa tttaatccac tatacctgcc cgacagccgc
     6061 aaagaccgta tcatacatag caggcattgc ggtgcgggat tactgccgca aacggcaggc
     6121 aaaaaaaata accgcttcaa ggcggttttt tggtggccag aggcggaatc gaaccgccga
     6181 cacacggatt ttcaatccgt tgctctacca actgagctat ctagcctttg tctgtctcgt
     6241 taagagatgc gtattaaacc aaattacggg acgacatgca agcatatcgg gcaatattct
     6301 ttgaaaacag gtttaagtca atgttttaaa tttgaattaa tttatgtaaa aacaacataa
     6361 atccgtattt ttgccgcctg cccgacgata ttaggtcgcc cgtctgaaaa atattttcaa
     6421 tatttgaagt tcttacccag ataaacagaa cgaacctgtt cgtttccgac caaatcatca
     6481 ggttttcccg atgccagcac cgcaccgtct gaaatgatgt aggcgcggtc gcagatgctg
     6541 agggtttcgc gtacgttgtg gtcggtaatc agtacgccga taccgcgcga cttgagaaat
     6601 ccgatgattt tttggatgtc gatgacggcg atgggatcaa caccggcaaa aggctcatcc
     6661 agcaaaataa aatgcggttt catggcgagt acgcgggcga tttcgacgcg ccgccgttcg
     6721 ccgccggaca gcgacggcgc ggggctgcgg cgcaagtgtc cgatattaag gtcggcgagc
     6781 agtttttcga tttccctgtc gatttgattt ttatctttgg ttctgatttc caagatggcg
     6841 cggatgtttt gttcgacggt cattttgcgg aatatcgagg cttcctgcgg caggtagccg
     6901 acaccgaggc gggcgcgttc gtgtatgggc aggtggcgca attcttgtcc gtctagggtt
     6961 acgctgcctg cgtcggcggc gatgagtccg acaatcatgt agaagctggt ggttttaccc
     7021 gcaccgttgg gcccgagcag accgacgact tctccgcttt cgatttcgag ggagaagctt
     7081 ttaacgactt ggcgtttttt gaaacttttt tgcaggtttt gaacaacaag gcggctgacg
     7141 tttgcgctca taaatatctc ttcaatatct tcggtcggca aatgccgtcc gaaccaggtt
     7201 tatgtttcag acggcatttt gggattattc ggatttttgc gtactcgaag gctggataac
     7261 gacgctgacc ctgccggatt tggaagcgga ttttgcgccg gattttgtgc tgccgctgat
     7321 ggtatagact tcggttttgg tgttgtatgt aatcaccgca ccttcggcga catcgccgcc
     7381 gcgctgtact ttggcattac cggttaagac tacggtgctg cctgcagatg aataagcaac
     7441 gttgtttgcc tgtccgcgca ccgtgccttt gccgccgtcc aatgtctggc tgaagcggac
     7501 tggcgaacct tccgccctca cggattcgcc gcctttgccg ccgcgtgtaa cattgacgcg
     7561 ggcggcggaa atattgagcg taccctgtct gatgacgacg tttccgctga atgtggtgct
     7621 ttggttggct tgatcgagcg aaccttggtc ggcctcaatc tgaataggct gcctgctgtc
     7681 gctttgaagg gcaaaagcgg gggacgccga aaaaaatgct attaaaacaa atagcttaca
     7741 tatcttttgt atcataaatc gtggctttca ctttagatga gaagttcaac atgcctgttt
     7801 tgtggtcgta agtcatgccg cccgcctgac cgtgcgatgc accatattgg aaactgacag
     7861 gcgtatcggt ttgggcatat tgagattcgg tatcgacgtg cagcttttcg gcttcaactt
     7921 tacccgcctg ccgtttgccg tcggcggttt tggtcagcac aacgttgttt ttaaaaagaa
     7981 cctgtttgtt ttcggtatgg taaacggctt catcgctgcc gacttcgtac aacaacctgc
     8041 cttcttggaa gaacacgaga tgcggtgagt cgaaatgaat gtcgctgctt tcgggaaact
     8101 gtttcgcgcc cttcgaactc aaatgttctt tcaagtatcc ctgttcgtca aaccgcctgc
     8161 catccaatcc gtccattgtg tattgcggtt cgtcgggatt gagcctgact tcttcaatct
     8221 cgacttcgct gatgcgtccc aaccatgccg acaggctgcc caaggcaacc gccaatatca
     8281 atgggaacgc aattccgtac cgccatctta ctttcatttg atgtactcgt tcaaagccgc
     8341 gcccaaagtc ccttgcgcct gcataatcag gtcgcacact tcgcgcaccg cgcccgcacc
     8401 gccggggcgt ttggcaatat aggcggcgtg ttgcagcgta aaccaatgcg cgtcggggac
     8461 ggcaaccggc aatccgcagc gtaccataac gggcaaatcg accacgtcgt cgccgacaaa
     8521 ggcacactcg gcttcttcca cgccgacctg agcgcgcagc tcttcatacg cggcgcgttt
     8581 gtccgaaatg cctttgaagt agtaatttat gcccaactgt ttgacgcgga tgccgacgga
     8641 gggcgcgtcc cgacctgtga taatcgcagt ctgcacgccg cttgcctgaa gcattttcag
     8701 accgtgtccg tccagtgtgt gaaacgattt gatttcttcg ccgttatcgc ggataaagat
     8761 gcgcccgtcg gtcaaaacgc cgtccacatc caggatcaac agtttgattt tggcggcgcg
     8821 cgcctgtaat tcgggagaaa ttgcctgcat gggatttcct tgttttcaga cggcatatct
     8881 taccacaaat gccgtctgaa acggctgcaa cgccttatac aatccgtgcc gccagcaggt
     8941 cgtgcatatt cagcgcgccg atcagcacgc catctgcatc ggtaaccaga agcccgttca
     9001 catggtttgc ctgcatgact ttcagggctt cggtggcgag acgttcggcg gagatggttt
     9061 taggatgcgt atgcatgact tcgtctatcg aaagaccggt aaaattgtcg cattcttgaa
     9121 acaggcggcg caaatcgccg tcggtgaata cgcctttcag acggccttgc ccgtccgtta
     9181 ccgccaacat gcccagccct ttttcactca tgctgacgat ggcttctttc aagggcgtgc
     9241 cgagtcggac ggcaggcagg ccgccgcctt tgtgcataat gtcggcaacg cgcaaaagta
     9301 ggcgtttgcc gaggctgccg gcaggatggc tcaaggcgaa atcgtcgggc gtgaacgcgc
     9361 gtgcgcgcag caggacgacc gccaacgcat cgcccaaagc catgacggcg gtggtgctgg
     9421 tggtcggggc aagccccagc gggcaggctt ctttggaaac cgacgccgtg atgtggatgt
     9481 cggcatggcg cgccatggtt gaatcggggc gggcggtgat gcagacaagc gtgatgtctt
     9541 tgcgtttgag cgcggggatg atggcggcga tttcgtcgct ttcgccggaa ttggaaatcg
     9601 cgacgaccac gtcgttgtcc acaatcatgc ccaaatcgcc gtgtgccgct tccgcagggt
     9661 ggacgaaaaa cgcgggcgtg ccggtcgagg ccatggttgc cgccattttg cgcccgatat
     9721 gtcccgactt gcccatgccc gtgataacaa ccctgccctt gcagtgcaac aacgcgtctg
     9781 ccgcaaggac gaagttttcg tccaattccg ctgcaatttc gcgcaagcct tccgcttcgg
     9841 tgtgcaacac ttcgcgcgcc cagtcgagat attttccgtt ttctgccatt gccatccgcc
     9901 tttgctgtaa caaaactacc caaaatttcc gaatcggggt agcataagca ttcaaacccc
     9961 gccatccatc caaaggaatc agaaatgact attttatcgg acgttaaagc attaggacaa
    10021 caaatctggc tggacaacct ttcccgctcg ctcgtgcaaa gcggcgaatt ggcgcaaatg
    10081 ctgaaacaag gcgtgtgcgg cgtaacttcc aatcccgcca tcttccaaaa agccttcgcc
    10141 ggcgacgcgc tttacgccga tgaaatcgcc gccctcaagc agcaaaacct cagccccaaa
    10201 caacgctacg aaaccatggc ggttgccgat gtgcgtgcct cctgcggcgt ttgccttgcc
    10261 gaacacgaat ccaccggcgg caaaaccggt ttcgtcagcc tcgaagtttc gcccgaattg
    10321 tccaaagacg cgcaaggcac ggtagaagaa gcgcgccgcc tctacgccgc catcgggtgc
    10381 aaaaacgcga tgattaaagt gcctgccacc gacgcaggca tcgatgcgct cgaaaccctc
    10441 gtttcggacg gcatcagcgt gaacctcacg ctgctgttct cacgcgccca aaccctcaaa
    10501 gcctacgccg cctacgcgcg cggcattgcc aaacgcttgg cagctggaca aagcgttgcc
    10561 catatccacg tcgtcgccag cttcttcatc tcccgcgtgg acggcgcgct ggacacaaca
    10621 ctgcccgacc acctcaaagg caaaatcgcc atcgcccttg ccaaagccgc ctaccaagac
    10681 tgggctcaat acttcggcag cccggaattt gccgcgctgg aagcccaagt cgcaaaccgc
    10741 gtgcagcttt tatgggcatc caccggcgtg aaaaacccgg cctatcccga cacactctac
    10801 gttgacagcc tgatcggcgc gcacaccgtc aacaccgttc cagatgccac gctcaaagcc
    10861 tttatcgacc acggcacagc caaagccacg ctgaccgaag gcgtagaaga agcacaagcg
    10921 cagcttgccg aaaccgccgc gctcggcatc gatgtcgaaa ccttggcggc acgtttgcag
    10981 gaagacggtt tgaaacagtt tgaagaagcg tttgaaaaac tgctcgcacc gttggtttaa
    11041 acttttcatc cccgatgccg tctgaaaggc tttcagacgg catcgcccgt ttgaccggac
    11101 acagtgcggc atatcaagga ttcaccctaa aaatattaat gggcaagaag ttaatgcacg
    11161 acattaccca ccaacacaaa aggaaataga atcatggcaa aagcactcga aatcatttca
    11221 cccaatgaaa tttattcaga tctgattttt aaggatccgg tacctcccca tactattgaa
    11281 tataaaatga ttaacctaga attgaaagcc atcaatcttt acgattgtgc ctttgaagat
    11341 ttcgttcccg aaatcaaaga caatttttgt gtgggaattg atttagacat aggaactaat
    11401 gatagtgatg ctgcagatat attttctgtt cgcatatgct ctcctaaatg gattttgcat
    11461 aattgtttcc aaaatcaaag ggttaaatgg ggagcgggta tgatgattat gaatgaattt
    11521 aatcattctg ttattaaatc ggaaattgag aaaattctta aagaatgttc aaaagaaact
    11581 tgggaaaagt cattgactta tttacttcgt tttttttcgt gggaatttga agattatcag
    11641 tgctaattaa atcaaaaaca gcgaaaccaa aactttttcg ctgttttatt tttccataaa
    11701 cttttcaaaa tatacagaat tgacgaaact ggttagtaga aatgcaagta atgagagaat
    11761 tgcagataga gcctttgatt ttttctcgcc tgctcttatg gatggttcag ctaccgaaga
    11821 gcaatataat gccctatatg atttaacctt gcttgaagag cccgggatgg aactgaataa
    11881 agatgagata atggctttga ttaatagtct taaataaagg ggctgtacca gatatgtaca
    11941 caggacgttt tgcccccagc ccgaccgggc tgctccacat cggctcgctg ctgaccgccg
    12001 tcgcttccta tgccgatgcg cgcgcgcacg gcggcaaatg gctgatccgc atggaagacc
    12061 tcgatccgcc gcgcgaaatg ccgggggcgg caagccatat cctgcacacg cttgaggcat
    12121 tcggattcga gtgggacgga gaagtcgcct atcagagccg ccgttacgcc ctgtatgaag
    12181 aaaccctatg ccgcctgaaa accgccggac tggtctatcc ctgccattgc agccgcaaag
    12241 actggcaggc cggggcaagg cggggcgcag acgggttcgt ctataacgga cgttaccgcc
    12301 accccggcca acgccctgca ctgcaaggca aacagccggc gtggcgcatc cgcgtccccg
    12361 accgcattat cggtttttca gacggcatcg tcggcggtta cgcccaaaac ctcgcccgcg
    12421 acatcggcga tttcgtcctg cttcgtgcag acggttactg ggcataccag ctcgccgtcg
    12481 ttgccgacga tgccgaacag ggcgttaccc acatcgtccg cggacaagac ctgctcgttt
    12541 ccacgccgcg ccaaatctat ttgcagcagt gtttggacgt tccgacaccg caatatgccc
    12601 acctgccgct gctgaccaac gcacaagggc agaaatggtc gaaacagacg ctcgcccccg
    12661 cattggattt aaaccgccgc gaacaactcc tccgccaagt gttccgttac ctcaagctgc
    12721 ccgaagcacc ggaaaccgac cgccctgccg aactgctcga ctgggcggtg gcacactggg
    12781 atatggacaa agtgccgaaa cacgccgtta ccgcccccta aacaccgccc gacaaacgca
    12841 caaatgccgt ctgaagcctt cagacggcat ttcacacata cagccccgcc ctattcgcgc
    12901 gtatttgccc gctcaacctc tttccacgct tcgagaatca ggctgccgtc gttgtctttc
    12961 aagagcgttg cgtcggaaag catacgccgc caagtccgcg cacctttcaa accgtgcatc
    13021 agcccgaggc tgtggcggac gatgtggcgc aagattgtgc cgcgtccggc ttggatctgg
    13081 gcttggctgt atgtgtagag gcactgcacc aaatcggcgt attcgatcgg gctgcgagta
    13141 tcgccgtaaa acaatctgtc ccattcgcgc atcaccatcg ggttgtggta cgcctcgcgc
    13201 ccgaccatca cgccgtcaac gtgttgcagg tgtccggcga ttgcttcgtt ggtggtgatg
    13261 ccgccgttga tgatgatttc cagcccggga aactcctgct tgaggcggta aacgtaatcg
    13321 tatttcaacg ggggaacgtc gcggttttct ttgggggaaa gaccgtccag ccaagcgttg
    13381 cgggcgtgga cgatgaaggt tttgcaggcg gttttgtcgc gcagcgtgcc gacgaaatcg
    13441 gcaacggttt ggtattcggt ctgcctgtcc acaccgatac ggtgtttgac ggtaacggga
    13501 ataccgaccg cgtcctgcat ggcgttgagg cagtcggcaa ccagcccgac ttcgttcatc
    13561 agacacgcgc cgaacgagcc tttctgcacg cgcggactgg ggcagccgca gttgaggttg
    13621 acctcgttgt aaccgtatgc ctcggcggct ttggcggctt tcgccaaatc ggacggatcg
    13681 ctgccgccca gttgcagggc gacgggctgc tcgccttcgt tgaacatcaa aaagcggtct
    13741 ttgtcgccgt aaacaatcgc gccggcgttg accatttcgc tgtacagcca agtattgcgg
    13801 gtaatctggc gcgccatata acggtagtgc ctgtccgtcc agtccaacat cggggcgacg
    13861 gacaggtggc gcgggggaag ggtgtgtgta ttgtcgttca ttgtcgatat tttatcattg
    13921 cggattcaat ttcaatccgg aacaaccttc atttttgggg aaatccgcgt catgaaagag
    13981 tccagcagta tggttaccgg cccgtcgttg cagagcgaca cctgcatatg cgtgcggaaa
    14041 cgccctgttt cgacatgaat tccgtgtccg cgcaacagtt ccgccgttcg caggtaaagc
    14101 tgctgcgcct gttctgcagg tgcggcttgc gaaaacgaag gccgccggcc gcttgtcgcg
    14161 tcggcataaa gcgtaaactg cgacaccagc agcaccgcgc cgccgacatc tttcaaagac
    14221 aggttcagct tgcccgcttc gtcttcaaac acgcgcaaat gggcgatttt gtcggcgata
    14281 tagcgtgcat ctttttctgt gtcgctatgc gttacgccga gtaacacgac aaacccgccg
    14341 tcgattttgc cacaggtttc cgtgccggct tcggacacga catccacctt tgcacctacc
    14401 gttttctgta tgaccgcacg cataagccgt cctccttacc tgaacgtgca atgccgtctg
    14461 aaaggcttca gacggcatcg gtattggaaa tgccattacg gtttgacacc gttttcttcc
    14521 aaaagggcat cgattttcaa gcgggctttt tcctgtctca aaccctgctc caactggttt
    14581 ctgaccaact cgaaaggctg cgcgtcgggg tttttcccga cctcgctgag tttgaacaga
    14641 taatagcgtt cgcccaattt gaccggatcg cgggtaacgt cgccccgatt catcgcggca
    14701 aactgcgaag ccagcggctc gggaagctgc tgcgccataa tgaaaccgtc aaaagcctgc
    14761 tcgtcgttcg gataacgctt catcagccct tcaaaagaca gccctttgag caggagctgc
    14821 tgcgcctgac gcgcctcctc ttcggttgcg aagctgacct gctgcaattt gatcatgcgg
    14881 atttgctgtt cgtaaaactt gtgcagctcg tcttcggaaa ccgtttccga acgttccaga
    14941 aaacggacgt actcctcggc ataaaaagac gcttcggcga ttttaaagcg gttttggaca
    15001 tccttatcct tatccaaacc ttccttcaat gccctgtttt tcaaaacttc caaagtttgt
    15061 agccggcgga cggcatcgtt tcggattgcc tgcccgtccg gtttttggga ctgctccgca
    15121 tgccggtctg cctgctgcat gatctgcgcc accagcgtat ccaccaaagc cgggtcgatt
    15181 tcgggtgctt tggctgccgc aaaacctgcc aacattgcag caataactgc ggcagcggtt
    15241 tttttctgtt tcatggtatt ccctgtcctg cgcggcggca aaacgcctgt ctgaacgtct
    15301 tgccgccata tcccgttttc agaattattt tgcaggtttg atgtttgcct tgcccaacag
    15361 tgcaccgacg gcacggtcaa tccgttccgc ctgaaggttg cccgcaatct gtcctttcat
    15421 ttcatcaaaa gaaggcactt ttacctcgcg gctgtcgttg acataataaa cgccgtagaa
    15481 atcgccgttt ttcagcggcg ttgccgtaaa ttcgcctttt ttcaagtcct taattgcctg
    15541 ataaagcggc ggaacaccct gttccaaatc tttcagcggc acatatccga ccggcgcacc
    15601 ggtctgtttg gtacggtcgt tgagggaata ttgtttcaag acggcatcga aacctttttt
    15661 cgccttcaag tcggcaaccg ctttttttgc attttcttcc ttgtcggtca ggatttcgcc
    15721 caactggact tcctgcgtac ctttgtaaaa accgctgata ttgtcatatg cggcttttac
    15781 ttcctgctcg gaaaccggtt gggttttggc gatatgcaat gcgtatgcct cgccgttcaa
    15841 gccatatttt accgcctgcc aaacggtttt gaaggacggt ttcttgtcgt cgcccgactt
    15901 tttcgcttcg gcacgcaatt tggcaagcgc atttttaaac tctgccgacc ggtcgagttt
    15961 caggcgtttc acttcctgtg cgaccacggt attgaccact tcgttttcca gcagggattg
    16021 gcgcagttgc ggcgtgtctt cggcacggct gttttccgca cggaatgcgg caacctgcgc
    16081 atcgatgacg gaactgtcga ttttctgacc gttgacggtt gccagcgttt gggcaaacag
    16141 gctgccggaa caggcaagca gtgcaacgga agtcaggatt tttgctttca taatcttctc
    16201 tctttaaatt ttaaattgaa atgttcgggc agacttcatt cagtccgccg tatggaaata
    16261 ctcgtcaggg gtagccgccc ggatgccgag cgcgtgaatc atgccgcctg aaaacaaatc
    16321 tttgagcagc gatttgaccg ttttctggcg gttcaggcgg cttacgcctt caaaacggcc
    16381 gctaacgacc aaaacggcat aatgtccgcc gccggtattg cccgcgtgtc ctttgtgcag
    16441 atggctctca tcgccgattt ccaacaccag cggatcgagc gtctgcaggc gttcgcggat
    16501 caaatcgacg gccggcatta tttgaacacc gctttaaacg gcttgatcag cacttcgctg
    16561 tacacgcctg catgaacata gggatcgtct tccgcccacg cctctgccgc atccaaagac
    16621 tcgaactgcg ccacaatcaa actgcccgaa acgcggttgg agtcctccgg caaaggattc
    16681 gggcctgccg tcaacagccg gccttccgac ttcagcgtct ccagccgttt gaggtgttcg
    16741 ggacgtgccg ccatacgcgc ctcgtgcaca tcctccccgt ctgttgccag caacataaaa
    16801 tattccacag tcaatcctcc tttttcagac aggtactcag ataaataccc tgaataataa
    16861 agaaaacaag catcagtgca gtcgagccga acattttata gttgacccat tgcgactcga
    16921 accgggtaaa cacaaaccag ttggcgatac ccataaaaat caggaaaccg acccacatat
    16981 acgtcaattt cgcccatacg gcatccggaa gctgaatctc cctgccgata ctcgccttca
    17041 agccgttttt accggcgagg tggctgcccc acaggaacag cgcgcccagc caaaacaaaa
    17101 cgctcggctt ccacataatg aagcggctgt cgcccaaaac aatggttgcg ccgccgaata
    17161 ccacaatcag caccaatccg acccactgca tcgtatccag ctttttatat ttccaataca
    17221 gaaaagccgc ctgaaccaca ccggcaacca atgcgaccgc cgttgcggca atcatgtttt
    17281 tggtaacggt ataggtggcg aaaaacagga tgacggacaa aaggtcgctg acgaatttca
    17341 tatccaaatg agaaagcaaa tcaaataaac gctaatcata caggcagaac cctgtttttg
    17401 cacctgcaaa accaacaaat tttgttttga ttgatgcaaa tatgcacaga tgtttttgaa
    17461 aaaagatgga gatatgtcat aatttgtaaa aacggcgaat gattgccgtt taaaatgtgg
    17521 cggcggtcgg tacattcaca tattaaatgc ccgccggccg actgccccgc cgatgcccga
    17581 tgatgacgaa atttacagac tgtacgcggt caaaccgtat tcagccgcca acccacaggg
    17641 gatacatctt gaaaaacaac agacaaatca aactgattgc cgcctccgtc gcagttgccg
    17701 catcctttca ggcacatgct ggactgggcg gactgaatat ccagtccaac cttgacgaac
    17761 ccttttccgg cagcattacc gtaaccggcg aagaagccaa agccctgcta ggcggcggca
    17821 gcgttaccgt ttccgaaaaa ggcctgaccg ccaaagtcca caagttgggc gacaaagccg
    17881 tcattgccgt ttcttccgaa caggcagtcc gcgatcccgt cctggtgttc cgcatcggcg
    17941 caggcgcaca ggtacgcgaa tacaccgcca tcctcgatcc tgtcggctac tcgcccaaaa
    18001 ccaaatctgc actttcagac ggcaagacac accgcaaaac cgctccgaca gcagagtccc
    18061 aagaaaatca aaacgccaaa gccctccgca aaaccgataa aaaagacagc gcgaacgcag
    18121 ccgtcaaacc ggcatacaac ggcaaaaccc ataccgtccg caaaggcgaa acggtcaaac
    18181 agattgccgc cgccatccgc ccgaaacacc tgacgctcga acaggttgcc gatgcgctgc
    18241 tgaaggcaaa cccaaatgtt tccgcacacg gcagactgcg tgcgggcagc gtgcttcaca
    18301 ttccgaatct gaacaggatc aaagcggaac aacccaaacc gcaaacggcg aaacccaaag
    18361 ccgaaaccgc atccatgccg tccgaaccgt ccaaacaggc aacggtagag aaaccggttg
    18421 aaaaacctga agcaaaagtt gccgcgcccg aagcaaaagc ggaaaaaccg gccgttcgac
    18481 ccgaacctgt acccgctgca aatactgccg catcggaaac cgctgccgaa tccgcccccc
    18541 aagaagccgc cgcttctgcc atcgacacgc cgaccgacga aaccggtaac gccgtttccg
    18601 aacctgtcga acaggtttct gccgaagaag aaaccgaaag cggactgttc ggcggttcgt
    18661 acaccttgct gcttgccggc ggaggcgcgg cattgatcgc cctgctgctg cttttgcgcc
    18721 ttgcccaatc caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa gagcctggcc
    18781 ttgacgacgc ggcagacgac ggcatagaaa tcacctttgc cgaagtcgaa actccggcaa
    18841 cgcccgaacc cgctccgaaa aacgatgtaa acgacacact tgccttagat ggggaatctg
    18901 aagaagagtt atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct tccaaccgca
    18961 tcgacttgga tttcgacagc ctggcagccg cgcaaaacgg cattttatcc ggcgcactta
    19021 cgcaggatga agaaacccaa aaacgcgcgg atgccgattg gaacgccatc gaatccacag
    19081 acagcgtgta cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc gtcatcgaca
    19141 cgcccgaacc ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc gtcgataccg
    19201 atttctccga caacctgccc tcaaacaacc atatcggcac agaagaaaca gcttccgcaa
    19261 aacctgcctc accctccgga ctggcaggct tcctgaaggc ttcctcgccc gaaaccatct
    19321 tggaaaaaac agttgccgaa gtccaaacac cggaagagtt gcacgatttc ctgaaagtgt
    19381 acgaaaccga tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac gccgccgcag
    19441 acgatttgtc cgcattgctt caacctgccg aagcaccgtc cgttgaggaa aatataacgg
    19501 aaaccgttgc cgaaacaccc gacttcaacg ccaccgcaga cgatttgtcc gcattacttc
    19561 aaccttctga ag
//
LOCUS       DAWKQJ010000023        19552 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000023 DAWKQJ010000000
VERSION     DAWKQJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.023"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            48..1226
                     /locus_tag="WJL20_000607"
     CDS             48..1226
                     /locus_tag="WJL20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ7682788.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(1293..1541)
                     /locus_tag="WJL20_000608"
     CDS             complement(1293..1541)
                     /locus_tag="WJL20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7682789.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(1632..2549)
                     /gene="ftsX"
                     /locus_tag="WJL20_000609"
     CDS             complement(1632..2549)
                     /gene="ftsX"
                     /locus_tag="WJL20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ7682790.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(2546..3196)
                     /gene="ftsE"
                     /locus_tag="WJL20_000610"
     CDS             complement(2546..3196)
                     /gene="ftsE"
                     /locus_tag="WJL20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ7682791.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3390..3872)
                     /locus_tag="WJL20_000611"
     CDS             complement(3390..3872)
                     /locus_tag="WJL20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7682792.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPVMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3996..4349
                     /gene="arsC"
                     /locus_tag="WJL20_000612"
     CDS             3996..4349
                     /gene="arsC"
                     /locus_tag="WJL20_000612"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ7682793.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4346..5002
                     /locus_tag="WJL20_000613"
     CDS             4346..5002
                     /locus_tag="WJL20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ7682794.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            5118..6512
                     /locus_tag="WJL20_000614"
     CDS             5118..6512
                     /locus_tag="WJL20_000614"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ7682795.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPADVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARSDGSPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIDANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMSKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6533..6928
                     /locus_tag="WJL20_000615"
     CDS             6533..6928
                     /locus_tag="WJL20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682796.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            6932..7423
                     /locus_tag="WJL20_000616"
     CDS             6932..7423
                     /locus_tag="WJL20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7682797.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(7546..10140)
                     /gene="mutS"
                     /locus_tag="WJL20_000617"
     CDS             complement(7546..10140)
                     /gene="mutS"
                     /locus_tag="WJL20_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ7682798.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAVEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTNLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            10584..11588
                     /gene="gap"
                     /locus_tag="WJL20_000618"
     CDS             10584..11588
                     /gene="gap"
                     /locus_tag="WJL20_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7682799.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11658..11777)
                     /locus_tag="WJL20_000619"
                     /pseudo
     CDS             complement(11658..11777)
                     /locus_tag="WJL20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11846..12526)
                     /gene="tsaA"
                     /locus_tag="WJL20_000620"
     CDS             complement(11846..12526)
                     /gene="tsaA"
                     /locus_tag="WJL20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ7682800.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12641..13276
                     /locus_tag="WJL20_000621"
     CDS             12641..13276
                     /locus_tag="WJL20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ7682801.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            13313..14281
                     /gene="waaC"
                     /locus_tag="WJL20_000622"
     CDS             13313..14281
                     /gene="waaC"
                     /locus_tag="WJL20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ7682802.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14508..15257
                     /locus_tag="WJL20_000623"
     CDS             14508..15257
                     /locus_tag="WJL20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ7682803.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIECGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15268..16203
                     /locus_tag="WJL20_000624"
     CDS             15268..16203
                     /locus_tag="WJL20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ7682804.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            16266..16877
                     /locus_tag="WJL20_000625"
     CDS             16266..16877
                     /locus_tag="WJL20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ7682805.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(16929..17246)
                     /locus_tag="WJL20_000626"
     CDS             complement(16929..17246)
                     /locus_tag="WJL20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ7682806.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17404..18675
                     /gene="purD"
                     /locus_tag="WJL20_000627"
     CDS             17404..18675
                     /gene="purD"
                     /locus_tag="WJL20_000627"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ7682807.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            18709..19311
                     /locus_tag="WJL20_000628"
     CDS             18709..19311
                     /locus_tag="WJL20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ7682808.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQAMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCLALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         4747 a         4408 c         5479 g         4918 t
ORIGIN      
        1 gcattaattg tttctgtatc gcaaacactg attgaaaagg actgattatg gcatttttaa
       61 aactgaccga acaaaacgtg cagggcaaaa ccgtcctcat ccgcgccgat atgaacgtgc
      121 cgttcaaaga cggcaaaatc agcgacgaca cccgtatccg cgcctcgatt gcgtccgtta
      181 aatactgcgt ggacaacggc gcatccgtta tcgtgatgac ccacttgggc cgcccgaccg
      241 aaggcgagtt ccaccccgaa gacgacgttg cgcccgttgc cgcgcatttg ggcggcctgt
      301 tgggcaaaaa cgtgaaagta ttgaacgact ggcgcgaaaa caaaccggct ttgaacgcgg
      361 gcgatgtcgt gatgctgcaa aacgtgcgta tcaacaaagg cgagaagaaa aacgatttgg
      421 aactgggcaa agcctacgct tctttgtgcg acgtgttcgt caatgacgcg ttcggcaccg
      481 cgcaccgcgc ccaagcctcg accgaagccg tcgcccaagc cgctcccgtt gcctgcgccg
      541 gcgtattgat ggcgggcgaa ctcgacgctt tgggcaaagc cttgaaacag cccgcgcgcc
      601 cgatggttgc catcgttgcc ggcagcaaag tgtccaccaa gctgaccatc ctcgaatcgc
      661 tggcggacaa agtcgaccaa ctcatcgtcg gcggcggtat cgccaacacc ttcctgttgg
      721 cggaaggtaa agccatcggc aaatctttgg cggaacacga tttggtggaa gaatccaaaa
      781 aaatcatgga gaaaatggcg gccaaaggcg gttctgtgcc gctgccgacc gatgtcgtcg
      841 ttgccaaagc ctttgctgcc gatgccgaag cggtcgtgaa agacattgcc gacgttgccg
      901 aagatgagat gattttggac atcggcccga aatccgccgc cgcgcttgcg gagttgctca
      961 aagccgccgg cacggtcgta tggaacgggc cggtcggcgt gtttgagttt gaccagttcg
     1021 caggcggtac gaaagccctt gccgaagcca ttgcccaaag caaagcattc tcgattgcag
     1081 gcggtggcga cacgctggcg gcgattgcta aattcggcgt taccgaccaa atcggctata
     1141 tttctaccgg cggaggcgcg ttccttgagt tcttggaagg caaagagctg cctgccgtag
     1201 ctgctttgga aaaacgcggc gcgtaagcgg tttgatataa aaaatgccgt ctgaaacgtt
     1261 gtgctgtttc tgacggcatt tttgtgtggc ggttattgtg ctttggctgc ccattccgcc
     1321 ggagtggcgg caaccgaaat ggaaagtgcg tgcagttcgt tactttccag ttgggctttg
     1381 agtccgtctt taatcaggcg gtggcgcgcg aggcgtgcct tgccttcaaa ttctgatgaa
     1441 acgatgacgg cgaaaaaatg gtgtccgtcg ccttctactt cgatatgttc gcattttgcc
     1501 acgccttcaa tcatggcttt tacttgttcc gaggtcagca tggtaggttc cttgttggaa
     1561 aaatgcgcca ttatactggc aaattgaaat gccgtctgaa aacgggttcg gacggcattt
     1621 ttgacggtgt tttatttttt ggctttgaag ccgagcaggt gctgggtggt ggcaagccac
     1681 gcgccgaata cgcccaacgc gatgacgaag ccgaacacca gtgcgatttc tcccgggtag
     1741 aagaaacgcc agccgatatt aagtccgtag ggtttgaaga tggcatcgac caaggggcgc
     1801 acggcagaga gcagccaacc gcaaagcccc aagctgacgg cggcggaaag gatgctctgc
     1861 cacatggctt gataaaggaa tgggcggcgg ataaacgacg cgggcgcgcc caagagtttg
     1921 gtgatttcga tttcttcttt gcggctgagg atttgcagtc ggatggtgtt gtgcgcgaca
     1981 aggacgaacg ccatccccag cgtcagggaa agaaaccata agattttacg gatgaattcg
     2041 ttgatttggt acagtgtctg tacccattcc gtatccatgt tggcggattc gaccataggc
     2101 agtttggtaa tgtcccggta gattgcctgc atttgggcgg gcgtggttgc cgggtcgggg
     2161 gtaacgataa agacatccgg cagcgggtta ccgtcaagca tggaaatcag attttggtcg
     2221 agattggact gtaattccgc caagccgtct tccttgccga tgaagcggac gttggcgatc
     2281 cgtttgtcgc gcgccagcag gctgcggacg gtatcgctgt cgctttgtgc ggcggcggtt
     2341 tccatataga cggtgatttg cggcgactcg ttgagtttgc ccaacacgct ttgcccgctt
     2401 tggatgccca aatacataaa cagcggcagg gtcatggcga cggcgagcat tatgagggta
     2461 agcagtgtgc cgaaaggttg gcgcagaagc tgtttgagcg cggtgcgcgc ggattcgacg
     2521 tgcagcgaga ggtagtggat gatgctcatg cgagtcgtcc tttcgagagg cgcaggatgc
     2581 ggtgtccgta gtccgccatc agggtttcgt catgtgcggc gacgatgacg gttgttcccg
     2641 cttcgtggaa ggttttgaac aattccataa tatcgagcgc gtaggcgcgg tcgaggttgg
     2701 cggagggttc gtcggcaatc agcaggccgg gctggtgaac gacggcgcgg gcgatgcaca
     2761 ggcgttgttg ttcaccgccg gagagggtta cgggatcgtc caattctcgt cctttcaggc
     2821 cgactttttc gatggcgatg cgggcacgct cttcggcttt gcgcggcgga tagccgataa
     2881 tccgaagcgg caggatgacg ttttgcagga cgttgcggtc gtagaggatt ttgtggtctt
     2941 ggaacacgat gccgatgtgt tggcgcataa agccgatttg gttgtcggtc agtgtgccga
     3001 ggtcttggtt gttgaagtgg actttgcccc tgcttggttt ggtaatgccc gaaatcagct
     3061 tgaggacggt ggatttgcct gaaccggagt gtcctgcgat aaaaatcatc tcgcctttgt
     3121 tgatttggaa gctgacgttt ttcagggctt caaaaccgcc gggataggtt ttggaaactt
     3181 gttcgaaacg gatcatagga ggtcctgtaa ggaagggcgg catatcgttt cgggcggttg
     3241 cgtccgcagg gatatgccgt ctgaacaata ggtaagacag tgccgattat aaagattccg
     3301 cctgctttta gatagtggat aagtttggcg gatatgaagg tcgggcggga aaaatgccgt
     3361 ctgaagcggc ttcagacggc atccggcgtt taacggcatt ttgaatgggc gagtttgacg
     3421 gcttcggtca ggcttttttc gttcacttcc ccggtaatgg tctgcctgta tccgcatttc
     3481 ggtgcttcga cgacggtaaa gggcagtacg ccgacagtgt ttccgtaggt tttcataaag
     3541 tttcggctgt tcgccccggt gtaacgccaa atcgggtagg aaacaggagt ttgtttgagg
     3601 aagttgccga tattgtcgga tgtgtcgagc gcgatgccga ccatatcgac gctgcctttt
     3661 ttctgggctt tgtaccattt ggacatcacc ggcatctctt tgcggcacgg gccgcaccaa
     3721 gttgcccaaa ggttgacgat gcggacgggg gctttgagcg attgcaggct ttgcgcggca
     3781 ccgtctttcc atccggccag ttcgtctgcc gaggcggtat gtgcggcaaa tgtggcggca
     3841 agggcgattg cggcaagatt caagtatttc atcgttttgt cctttgtatg tcgattcggg
     3901 tattgtaaac cgtttggtac ggttggaggc gggatattga gtaaaatcct gcgtaagccg
     3961 ataataaggg gattgtcgag aagtaaggaa atgtcatgtc tgaaatcaaa attttccaca
     4021 atccccgttg cagcaaatcg cgcgccgcct tgtccctatt ggaagaacgc ggcattgctg
     4081 ccgaagtggt caaatatttg gatacgccgc ccgacttgtc cgaattgaag gatattttca
     4141 acaaattggg tttggcatcg gcgcgcggga tgatgcgtgt gaaagatgat ttgtacaagg
     4201 aattgggttt ggacaaccct gatttggaca atgacgcgct gctgcgtgcc atcgccgatc
     4261 atcccgccct gttggagcgt ccgattgttt tggcaaacgg taaggcggct gtcggcagac
     4321 cgctggaaaa tatcgaagcg gtattgtgaa caaacggctg ttttgcagcc gcaacggctt
     4381 gcgctattac ctgttgggcg gtttctgttt gagtgttttt ccgcttttgc tggtgtttgc
     4441 ctcatccgtt tgggcggttt atcggacggg cgggcaggtt ttgccgccgt atgtccgggc
     4501 ggatgccgcg ctggtgttgg gcgccgccgc gtgggacaaa cgcccttccc cggttttccg
     4561 cgaacgcatc aaccacgcca tcgcgcttta tcagagccgc agggtaggca aaatcgtgtt
     4621 tacgggaggg cgaaccaaaa aaggctatat gaccgaagcc gaggtcgggc ggcggtacgc
     4681 gctcaaacag ggggttccgg cgcgcaacat cctgtttgaa aacacttcgc gcaacactta
     4741 tgaaaacctg aacaacatcc gtccggtttt gcgtgccaac ggtattgaca gcgtggtgat
     4801 tgtcagcgat ccctaccatt tggcgcgggc ggcggaaatg gcggaggatt tgggtgtccg
     4861 cgtttatatg tctgccaccc cgaccacgcg gtttgatgcg ggaaataaga aaaaaatctt
     4921 tatgctgcaa gagggatatg cgttatccct ctaccgtttg gaaaaatggg gcagccgctt
     4981 tttgggatgg ctgtctgatt gaagcggtag aaaatgccgt ctgaaactta tccgcaggca
     5041 gggttcggac ggcatttttg atgcccaagc acggtaaaac cagtaaaata aacgctttca
     5101 aagatttcgg agccatcatg accgtcaaaa cccgtttcgc ccccagcccc accggctacc
     5161 tgcacattgg cggcgttcgc accgccttgt tttcgtgggc gtttgcccgc catcataaag
     5221 gcgaattttt attgcgcatc gaagacaccg acttggcgcg ttctaccgcc gaatccgtca
     5281 acatcatcct cgacggcatg aaatgggtcg gcctgaacta cgacaacgcc gacaatgtcg
     5341 tgtaccaaac ccgccgtttc gaccgctaca aagaagtcat tgccgaactt ttagaaaaag
     5401 gccatgccta ctactgctat tgcagcaaag aagagctgga agccatgcgc gagaaagccg
     5461 aaaaagaagg cacggcgact tacgaccgcc gctggcgacc ggaagcaggc aaaaccctgc
     5521 ccgaaattcc tgccgacgtg caacccgtcg tccgcttcaa aacgcctttg gacggcgtta
     5581 ccaaatgggc ggacctggtc aaaggcgaaa tttccatccc caatgaagcc ctcgacgacc
     5641 tgattatcgc ccgatccgac ggctcgccga cctacaactt ctgcgtcgtg gtggacgact
     5701 acgacatggg cgttacccac gttatccgcg gcgacgacca tgtgaacaac acgccgaaac
     5761 aaatcaacat cttaaaagcc atcgacgcga acctgcccga atacggccac ctgccgatga
     5821 ttcttaacga acaaggcaaa aaaatctcca aacgcagcgg cgataccgtt gccattaccg
     5881 atttcggcgc aatgggcatc ctgcccgaag cgatgctcaa ctatctggca cgcttgggct
     5941 gggcgcacgg cgacgatgaa ttcttcacaa tggaacaatt catcgaatgg tttgatttga
     6001 aagacgtttc cccgtctcca agccgtatgg acttgaaaaa actctactgg atcaacggcg
     6061 aacacatcaa aatcaccacc aacgaaaaac tggccgaaat ggtgaaaccg cgcctcgccc
     6121 tgcgcgatat ccatgaaaca agcaagcctg ctttggaaga cgtgttggca ttggtcaaag
     6181 accgcgccca agatttgaac gccttggcag acgaatgtct gtatttctac aaaaaacaag
     6241 tccctgccga agccgatgtt gccaaacact gggacgacga agccgccgct cgtatgctgc
     6301 gctttgccga acgcctcgaa gggctggaag actggaatgc cgaagccatc cacgacctct
     6361 tcaaaccctt ctgcgacgaa gaaggcatta aaatgagcaa actcggcatg cccctgcgcc
     6421 ttgccgtctg cggtacggcg aaaaccccca gcgtcgatgc tgtgttggca ttaatcggca
     6481 aagaagaagt gttgaaacgc atccgcgctt aagttatcca caggcgtggg cgatgccgtc
     6541 tgatgtcgga atgcggcttc agacagcatt caaacggaag ttgaacatga aaaaaatatt
     6601 gtattttctg atgtttgttt tttctacaag cgtatgggcg ggggatgcgg aagacaatct
     6661 gctcagcatc caatccggct atcgtgcctt actgcaaaaa caaaacaatc tggacggaaa
     6721 aatcatcggg atgcagtcgg atttggaaga tgcgcgccgg cgtttgcaga cggctcaggc
     6781 agacatcgcc cgtttggaag cggaaattcc tgcagcaatg gcgcaaaaag cccggcaggc
     6841 tgaagagctg aggcaaatcg gagtgcgttt ggaccatgct tggaatgcgg tttacggcgc
     6901 agggggaacg aaagcgtcgg ggaattgacc gatgaattcc ctctttgtag acaatactgt
     6961 tttcattaca cggctgaaag ccgggcatat cggcaggttg gttcaggcgt tgtttgagga
     7021 gtggcacgga tttgaaccgt ggtcttctgt ggataagatt catgcctatt acggcaggtg
     7081 tttgaaggat gacgaactgc cgctggcttt tgcggctgtg gatgattccg gaaccctgtt
     7141 gggttcggct acggtcaagc ggcatgatat ggaaagtttt ccacagtatg aatattggtt
     7201 gggtgatgtc tttgttttac ctgaatatcg cggaaaaggc attggcagga ggctggtcgc
     7261 ccactgcata ggcgcagcgc gttcgctggg gataaagttc ttgtatcttt atacgcctga
     7321 tgtgcaaata ttttatgaat cattcggctg ggcggttgtc gggcgacatt tccataacgg
     7381 tgaatgggtt acggttatgc gtttggatat ggataaggtt taagccgaat ggacaaatgc
     7441 cgtctgagct gttcggacgg catttttatg tttggaaact tcttgtcaga taatgtttat
     7501 ccacagattt ttccatatgg tttgatgctg ttccgccaac ggaaattaag atacggattt
     7561 gcacaaatct ttcagacggt acagttctga caatgcttcg cgcggggtca ggctgtcggg
     7621 atcgagtttt tccaaggctg ctgccaatat accttcaaaa tgtttttcct ctgctttgtc
     7681 cacaaagttg cccacattcg gttcatctcc tttttcagac ggcatggtac tgaaaatatc
     7741 cagttgggga cggtttgcgg cggcttggtt ttccagtccg ttcaaatgct tttgggcgga
     7801 tttcaatgcg cgtacaggca ggccggcgag tttggcaacg gcgatgccgt agcttttgcc
     7861 ggcgggaccg ggttggattt ggtgcagaaa cacgatgtcc tgtccctgtt cgagcgcgga
     7921 aaggtgcata ttgagtgcgg cggcgtgggc ttcgggcagg taggtcagct cgaaatagtg
     7981 ggtggcaaac aggctgaagg atttgttttt ttgcagcagg tgttcggcaa cggcgtgcgc
     8041 gagggcgagg ccgtcgaaag tggaagtgcc acgtccgact tcgtccatta aaacaaggct
     8101 ttgttcggtg gcgtgatgca ggatgtaggc ggtttcgctc atttcgacca taaaggtgga
     8161 gcggttggag gcgaggtcgt ccgatgcgcc gatgcgggtg aagatttgat cgatgggccc
     8221 gattgtggcg gcatcggcag gcacgaaaca gccggtgtgt gccaataaaa caatcagcgc
     8281 gacttggcgc atgtaggtgg atttgccgcc catattgggg ccggtgagca gcatgaggcg
     8341 gtgtttgtgg tcaaggttgg tgtggttggc ggtgaagtgg cgtacctgct gttcgacaac
     8401 gggatggcgg ccgttttcga tgtggataac cggatagtcg gcaaactcgg ggcggacgaa
     8461 gttccgctct tttgccaagg ctgaaaatgt ggacaacacg tccagcgcgg cggcggcttt
     8521 ggcggctttt tgaagctgcg gcaatgccgt ctgaaggttt ttcaatacgc cgtcaaagag
     8581 ttgtttttct aaggcgaggg cttgctcttg agcagtcagc actttgtctt caaaggcttt
     8641 caactccggc gtgatgaagc gttcggcgtt tttgagggtt tgccggcgtt ggtagtcggc
     8701 aggtgcttgt tcggcttggg ttttggacaa ttcaatgtaa aagccgtgaa cgcggttgaa
     8761 ctcgactttg agtgtggaca aaccggtacg ttcgcgttcc ttggcttcca aatccagcaa
     8821 aaattcgtcg ccatggtttt gaatgcggcg caattcgtcc agttcgggat gaaaaccgtg
     8881 gttgatgaca ttgccgtctt tcagccagac ggaaggttcg ggcaaaatgg cttggcggag
     8941 ctgttcggct gtggataggt tttccgggaa aacggctttg agggttccta agagactgct
     9001 gcactcggcg gacaattcga tttcggacag ggcaaacagg ctgtcgcgca gggcggcgag
     9061 gtcgcgcggg cgggcgttac ccacggcaat acgggcggcg atgcgttcga tgtcggcaat
     9121 gcttttcaga cggcactgga ggggtttgta ttggctttcc agcgcggcaa cggcttcttg
     9181 gcgcgctcgg atgtgggcgc ggttgcgtaa agggtggtgc agccagagag ccaagaggcg
     9241 gctgcccata tgggtagcgc aaaggtcgag cgtggacatc agggtcggcg attttttgcc
     9301 ggagagggtt tgcgtgattt cgagattgcg gcgcgtggcg gcatccatac cgatatattg
     9361 gctgtcggtt tcgagcgaca ggccgtccaa atgttgcggc atcaggtttt gcgtcagacg
     9421 gatatagttc aacagtgcac ctgccgcgcc aatcgcaacg gcgtgttctt tgccgtccaa
     9481 accgaagccg cgcaaatcct ggcagccgaa atattccgtc agcagttttt cccccgcgtc
     9541 ggcggcaaac tgccacgcgt tcaggcgcgt aacacccgat gccgtctgaa gttgcggtgc
     9601 gtttttactg tcaggcaaca gaatttccgc cgcctgcagg cgcgccagtt cgtcgtccaa
     9661 tttatccaca gttgtcagct tggttttgaa ttcgccgctt tgcagcgatg cccacgccaa
     9721 accgatgtat tttttgtcgg gggacacggc aacgatgcgg ttggtttcct tgtcttccag
     9781 caatgcggaa tcggtcagcg tgccgggcgt tacgatgcgc acgactttgc gctccaccgg
     9841 ccctttgccc gcgccgactt cgccgacctg ttcgcaaatc gccacgcttt tgcccaactt
     9901 gaccaggcgc gccagatatt gttcgacggc gtgaaacggc acgcctgcca ttttgaccgg
     9961 ctcgccatcc acctgtccgc gcgtggtcag ggtaatatcc aaaagttttg ccgcttctac
    10021 cgcatcgtcg aaaaacatct cgtaaaaatc gcccatacgg taaaacacca gtttgtcggt
    10081 atgttgcgct ttgatgccga ggtattgctg catcattggg gaaacggcgg atttggacat
    10141 ggggctgcct tatcattagg aaaacggtat tgtagcaaaa ggttttgtct tcgacggaat
    10201 gagactgacc gtaagcctgt caataaggtt gtggagacaa ctaggaaaac agcaaagtta
    10261 tccacagaaa tattattaag tcaaaaaaca tccacgctta tctgtcttat acagcaagtc
    10321 ttaaactcaa acttatttga caaaaaggta atgaaaatat tggatatttg ctacttacgc
    10381 acaagcaggt tggcaatcat caaaatcatc attaaaattt taaattcaaa tattgatggt
    10441 aaaagttttg gaaatctgtg caaacagtaa aattcatcta tatcaaataa gcatcaagta
    10501 gttagatgta atagtggaat gtaactttat ttcagaatac aatacaggta ttgaaatttc
    10561 gtttcataac ttggagcata aacatgagca taaaagtagc gattaacggt ttcggccgta
    10621 tcggccgcct cgcattgcgt caaattgaaa aagcccacgg catcgaagtc gtcgccgtca
    10681 acgacctgac ccctgccgaa atgctgctgc accttttcaa atacgacagc acacaaggcc
    10741 gtttccaagg tactgccgaa ttaaaagacg atgccatcgt ggtaaacggc aaagaaatca
    10801 aagttttcgc caatccgaat cccgaagaat tgccttgggg cgagttgggt gtggatgtcg
    10861 tcctcgaatg taccggcttc tttaccaata aaaccaaagc cgaagcccac atccgtgccg
    10921 gcgcgcgcaa agtcgtgatt tccgcaccta gcggcaatga tgtgaaaacc gtcgtatatg
    10981 gcgtgaacca aaacattttg gacggcagcg aaaccgttat ctccgctgct tcctgcacca
    11041 ccaactgcct tgccccgatg gcggcagtcc tgcaaaagga atttggcgta gtcgaaggcc
    11101 tgatgaccac catccacgcc tacaccggcg accaaaacac ccttgacgcg ccgcaccgca
    11161 aaggcgattt gcgccgcgcc cgcgccgccg cgctcaacat cgtgcccaac agcaccggtg
    11221 ccgccaaagc catcggtctg gttatccctg aattgaacgg caaactcgac ggctccgccc
    11281 aacgcgtccc tgttgccacc ggctcgctga ccgaattggt ttccatcctc gaacgccctg
    11341 taaccaaaga agaaatcaac gccgcgatga aagccgccgc cagcgaatct tacggctaca
    11401 acgaagatca aattgtttct tccgacgttg tcggcatcga atacggttcg ctcttcgatg
    11461 cgacccaaac ccgcgtgatg accgtgggcg gtaaacaact ggtgaaaacc gttgcctggt
    11521 acgacaacga aatgtcctac acctgccaac tcgtccgcac tttggaatac tttgcaggca
    11581 aaatctaagg cttgaacaaa cctgtggata agtaaatgcc gtctgaacac attgcgttca
    11641 gacggcattg tttatctgta tagtggatta aatttaaacc agtacgacgt tacctcgcct
    11701 tgccgtacta tttgtactgt ctgcggcttc gttgccttgt cctgatttaa atttaatccg
    11761 ctataacttg atattgcggc ttatggcgaa caaacaacag ccgtttgttc aactgctatg
    11821 cggcaaaacc ggatttttgc ccgatttaaa gcggggttgg ggaaagatca ataacggttg
    11881 cacggttttc ctcgatttga aatctgactt cgtaatctgc aatattcatc acataaatcc
    11941 gttcgggaat attctgatag gcggggcgcg gatcttgggc aatgctttgg ctgataaggt
    12001 ttttggtgtt tgcagataaa ttttccgcgc cgatgttttc ctgccaaacg acttccaact
    12061 ctacgggttt gccgctgacg aaaccagatg cggcatcggg tttggattcg acaaagggga
    12121 tataaggctt gatgtccaca atcggcgtgc cgtcgagcag gtctgcgccg ctgcaataaa
    12181 ggcggacggg tttgccggtt tcgatgcggt tgagttttaa gagtgatagt ccgagatggt
    12241 tggggcggtg ggggctgcgc gtggcgaata cgcccatttt ttgtttgccg ccgagccgtg
    12301 gcgggcgcac catttgtgcc cagccttcat ccaatacgcc gtggaagata aaacttatcc
    12361 acacataatc gaaatcttcc agcccgcgca cgctgtctgc ggtaaattcg ggattcagct
    12421 cgatgcatac ttctgcggca gaggctaaac cgggctggcg ggcgatgccg aatttctgtt
    12481 tgtagggcga tcgggcggtg ccgatggggg taatggtata agtcataagg ttgtggataa
    12541 gtttttttgg aaaaacctta tcttatcaca atcggtttta ccgtttttgg gcagtaattt
    12601 gttataatac agggcatttt aatcgcgttg aaataatgag atgattgttt ccattgatgt
    12661 tgatgcacaa aaaacgttta cgccactgtg tcctgacgaa ctgcccgtga acgaggggca
    12721 tttgattgtc gaggagttga atgctcaagc cgctttggcg gatttgcgcg tgatgacgaa
    12781 agatgcgcat catatggtag cgaaatggct tgtggataac cctgttgata tgttgaagcc
    12841 gacaggtttg cctgatgcgg atttgacttg ggtggctcat gcgatggtcg gtacgcgcgg
    12901 ctatgaatta ttggacggac tgccttctgc caaagaatat gattattgcg tttggaaagg
    12961 tgttgatcct gaattgcatc cttacggcgc gtgttttcac gatattgagg aaaaactaag
    13021 cacagggctg attgaatggc tgcgttgtca aaatacggat acggttatta tcggtgggtt
    13081 agctacggat tattgtgtta aaacaacggt tttgcagtta ctcaaaggcg gtcgttggca
    13141 ggttgtcgtc aatgaagcgg cttgtcgggg tattgcgccg gaaaccatcg aaacagcatg
    13201 gcaggaaatg cattctgccg gggcaatcat cttaaaaaac gctgatgaaa taaataaata
    13261 tattaataat caataagtta aaattgtttc acgtgaaaca cccttcctaa atatgaaaat
    13321 tttgcttgtc cgcttgtcta gtatgggcga tttaattcac actttgcccg caatcgaaga
    13381 tttggcgcga caatgtcctg atgtggaact gcattggcta tgtgaggctg gatttgcaga
    13441 tattgcgcgc ctgcatccgt ttgtaaaaaa aatccatgtg atgaaatggc ggcaatggcg
    13501 caaacatctc tttcgggctg aaacttggcg ggaaatgggt catctgaaac agactttgcg
    13561 gcaggaagta tttgatttcg tattggacag tcaaggtctg attaaaagcg cgtgtttcgc
    13621 taaaatggca aaatccccga tttgtggttt ggataaaaac agtgcgcgcg agggatgggc
    13681 tgctttggcg tatgtaaaaa catacgctgt accgaaggga aaaaatgccg tttggcgcaa
    13741 ccgtgaactg tttgcccaag tatttgggta tgtaatgccg gaaacgcagg tatttggctt
    13801 gactgttcct gaagcaggcc gtctgaaaaa tttagcgcag ccgtattatg cggctttgca
    13861 tgcgaccagt cgggacagta agttatggcc tgtggaaaac tggcggtcgt tgctgcaaaa
    13921 gttgaatgaa gaacagcaat gcaatattta cctgccttgg ggaaatgaag atgaaaaaac
    13981 gcgtgccaaa caaattgcag atgggctgcc gtttgctatt gtgtgcgaca aaataaattt
    14041 attgcaggca gcgtatctgc tgaaatacgc ggtcggaatt gtcggcgtgg ataccggttt
    14101 gctgcatttg gcaaatgcct tggaaaaacc tgtggtcggc atttataccg ataccgatcc
    14161 gattaaaaca ggcgttcagg tttcgccaat tgcaaaaaat ttgggcaata tcgggcagat
    14221 tccgaccgca gatttggttt atcaagcgct gatggattgt gttgcagcag ataaaggcta
    14281 aagggtcgtc taaaactgat atggcggcat gatattttcc aatttattag atacaggaat
    14341 gtatatccgc tagtctgagt aaatgcttta aaaatggtat aatcggcaag cattatccga
    14401 aagatttttg aatgaattcg tttttaagga aaacggattt ggttttatag ggaagaaggt
    14461 ttggtttcgc tggatggtga aagcagcgtt ctttttagga gggttcaatg aaagcactgg
    14521 tcgcagtaaa gcgcgtagtg gactacaacg tcaaagttcg tgtaaaagcc gatggttcgg
    14581 atgtggatat cggcaatgtc aaaatgtcga tgaatccgtt tgacgaaatc gctgtggaag
    14641 aagccgtccg tttgaaagaa gccggaaaag taagcgagat cgtagcggtt tctttgggcg
    14701 aaaagaaatg cgaagaaacg ttgcgtacag ctttggcgat gggtgccgac cgtgcaattc
    14761 atgttgaaac cgatgcgaaa ttggagccgc tggcagttgc caagttgctg aaagccgttg
    14821 cggacaaaga aaatccgcag attttctttt tgggcaaaca ggcgattgat gatgatgcca
    14881 atcaagtggc gcaaatgctg gcagctttgc tgaatgcggc gcaaggtacg tttgcttcaa
    14941 aagtacacat tgaatgtggt gaagtacaga ttgtgcgcga aattgatggc ggcgaagaaa
    15001 ccatagcatt aaaactgcct gctgttatca gtgctgattt gcgtttgaac gagccgcgct
    15061 ttgtcaaact ccccaatatt atggcggcaa agaaaaaacc tttggaaaaa ctgactcctg
    15121 acgatttggt tgccgacatt tcacctcgtt tgaaaacggt gaaatttgcc gaacctaagg
    15181 cgcgtcaggc aggcgtaaaa gtagcaagcg ttgccgaatt ggttgaaaaa ttgaaaaacg
    15241 aagccaaagt gatttgagga ggctgaaatg agtgtattga ttattgtcga acacgacaac
    15301 aaacagttga atcctaccac tttgcatgct gttaccgctg ccgctaagct gggtaaaatc
    15361 gatttattgg ttgccggaag cggtgcatct gctgtggtag agttcgcaaa acaagtggcg
    15421 ggtgtggaaa aagttttggt tgcagatgct gcccattatg ccgaaggttt ggctgaagag
    15481 ttggctccgc tggttgttaa attggcagcg gattactgct atgttgcagc aacggcaact
    15541 acattcggta aaaacctttt gcctcgcgta gcagccttat tagacgtacc gcaaatttct
    15601 gatttgaccg aaattgtgga taacacgact tttgtgcgcc ccatttatgc gggtaatgca
    15661 tttgaaaccg tgcaagctga ttcagaaaaa ttggtgctga ccttccgtgc gacggttttt
    15721 gacgcagtgg cggcgcaagg tggtaatgct gaggtaatta atgttgaagc aacccctgcc
    15781 caaaacctga gtcgttttgt taaccgtcag ctttcccatt ccgatcgtcc tgaactgact
    15841 caggcaaaag tgattgtttc cggtggtcgt gcgttgggta gtgcggaaaa attcaatgaa
    15901 gtgctgacac cgttggcgga tgttttaggt gcggcaatcg gtgcatcccg tgcagcagtt
    15961 gatgccgaat atgcgccaaa cgatgctcaa gtcggacaaa ccggtaaagt ggttgcgccg
    16021 caactctatt ttgcaatcgg tatttcaggt gcaattcaac acgttgccgg tatgcaagac
    16081 agtaaggtga ttgttgcaat caataaagat gctgatgcgc cgattttcaa tgtagccgat
    16141 tacggattgg ttggtgattt atttgaaatc gttccacaat taacagaagt attgaaaaat
    16201 tgatgtttca cgtgaaactt tcagacgacc ttgttattaa tgaggtcgtc tgaaaacatt
    16261 tcagcatgat tacgacttat aaaaccatta cttcctcgac gcaggctgag tttaaagata
    16321 aaggcagccg ttttattgca tttgcctatc cgattcggac attggctgat gtgaaaaaat
    16381 acctcgatcc gttaaaggaa gagcatcata aagcacgaca ctggtgctat gcctatcgtt
    16441 tgggtgtgga tggtgtgcaa tttcgtgcca acgatgatgg agagccatca ggaagtgctg
    16501 gacgaccaat tttgggacag attgattcgg tgggtattac cgacgttttg gtcgtggtcg
    16561 tccgctattt cggtggtact ttgttaggtg ttccaggtct gatacatgcg tacaaagagg
    16621 cgacggctca agcgttggca gttgcagaag tagttgaaaa gaatattgaa aaaactgttt
    16681 ggctgaaatg tgaatatccg tttttgaatg aagcgatacg cattgctaaa caatatcagg
    16741 cggatgtatt ggagcgggat ttgcagttgg attgccgatt gacggtcagt ttgtcgttgg
    16801 taaattatga ggcctgtgtt gcggcttgga agagtacacg gcagattgaa gtaaatacgg
    16861 aaaagccttt tgaatgaaag gtcgtctgaa atgctttgtg atggtatttc agacgacctc
    16921 ttcgctgact aatcttcttg tgtatatttg tctaaggcat cgacgctgga gcgcaaatat
    16981 aaccaagcca gttgatggaa ttcgcccagc gcgttccata gtgcatcttc actcataatg
    17041 ctgtattcgc cttctgtgcg caaatccacc tccgctccat cttcaatagc ccgatgacaa
    17101 gcctgatatt cgtgggtata aaaattgtcc atatcgcgaa tcaaagcgac ggcatgtttc
    17161 caacggcgga tgtcttcttc caattgcggt agtaaatgac accattcgcg gtatttgctt
    17221 tggatttcat caatacgttt ttgcatggtt gcttgtcctt ttttaattta tgaataagtt
    17281 gaataggttg cagagtggaa tgaagcggtc ttttgtttga ttctactaaa atttcttcag
    17341 atgggataaa agtccctttg tatcgagttt tcgttaaaag tacatatttg tcaaggagat
    17401 ggcatgaaac tgctggttat cggtaatggc ggtcgcgaac acgcgctggc ttggaaattg
    17461 gcgcagtcgc ctaaagtgga aacagtattt gttgcgtctg gtaatgccgg tacagcgatt
    17521 gaacccaagc tgcaaaacat cgacttgact gcgcatcagg atttgattga attctgtcgt
    17581 aaagaaaata ttgtttttac cgtcgtcggc cctgaagcgc ctttggcggc aggtgttgtg
    17641 gatgatttcc gtgctgcagg actaaaaata ttcggtccga cacaatatgc ggcgcagttg
    17701 gaaagttcca aagatttcgc caaagcattt atggcgaaat acaatattcc gaccgcgcaa
    17761 tatcaaacct ttgaaaacgc cgatgccgca catgattacg tcaatcagaa aggtgcgcct
    17821 atcgttatca aagccgatgg tttggcggca ggtaaaggcg tgattgtggc gatgactttg
    17881 gatgaagcgc atgctgcgat tgatgatatg ctgctggaca acaaaatggg caatgccggc
    17941 gcgcgtattg tgattgaaga tttcctgcaa ggcgaagaag cgagttttat cgtcatggtt
    18001 gatggcaata atgtactgcc tatggcaacc agccaagacc acaagcgtct tttagacggc
    18061 gacaaaggtc tgaatacggg cggtatgggt gcgtacagcc ctgcgcctgt ggtaacgcct
    18121 gttgtgtacg aacgcgcgat gaatgaaatt attttgccga ccgtagcagg gatgaaagcg
    18181 gaggggcatg agttcaccgg tttcctgtac gcaggtttga tgattgatca aagcggtgca
    18241 ccccatacga ttgagtttaa ctgccgtttc ggcgatcctg aaacccagcc gattatgagc
    18301 cgactgaaca gcgacttatc ggatttggtt gaagcggcaa tagacggcaa actcgatagc
    18361 gtaaccgcgg aatggagccc gcaaactgcc gtgggcgtgg tactggcggc gcaaaattac
    18421 cccgaaacgc ccaaaaaagg cgatgttatt tccggtctgg atgctgccaa ccaagtaggc
    18481 aaagttttcc atgcaggtac aacggcaaac gagaaaggtg acgtattgac caacggcgga
    18541 cgcgtattgt gtgttgtggg attgggcgat aatgtggcgc aagcgaaagc taaggcatat
    18601 ggcgcgttgg aaaaaatcag tttcgacggg atgcaatacc gtaaagacat tgccgataaa
    18661 gcgattaacc gttaattaga aagaggatga tgccgtctga agccaagtat gactgatgtg
    18721 cttcagacgg tattttatat tatgttattt ccgagaatta tcgcagcatc agcgcaacgt
    18781 aagttaagcg tttatttgaa aaaaggcggt ttggtcgctt atccgaccga atcttgttat
    18841 ggcttggggt gtcttccgac attagccaaa gcactcggta agttggcgca tttgaaaaaa
    18901 aggccgcaac acaagggtat gattgtcatc gggaatcaat ttgaacagtt gcagccttta
    18961 cttcaaatgc cgtctgaaaa tctgcaggct atgcttagaa aagaatggcc cgcccctaaa
    19021 acattcctat tatctgccaa atcctgcgta ttacctgagt tacgcgggaa acagcgcagc
    19081 aagttggcgg tcagggttcc cgcccatgta ggtgcgcgcc gcttgtgtct ggctttgcaa
    19141 acgcctttgg tctcaacttc gtgcaaccgc gcaggcaaac gcgcgtgtcg gacggagagg
    19201 gaagtcagaa ggcaattcgg acgcgatgtg tggattgttg gtgggcgaat cggcaggcag
    19261 aaatcgccga gtcagattat tgacggggaa acaggtaaac gtttgcgtta gatatgggta
    19321 cttgaatgaa aacttttttt gcatacacaa agaaaaaatt tttcggatag catttctcta
    19381 catgattgtt attgcacaga gataaaggta gaaggacgca acgtctgctt taattttgag
    19441 gacggttttg tcgttccgga caataatccg aacaatcaag cgggcaagca tctgaaaaca
    19501 gatttttccc gtgttgtttt aatccataaa aatgaaaacg ccgaagatga tt
//
LOCUS       DAWKQJ010000024        18781 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000024 DAWKQJ010000000
VERSION     DAWKQJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.024"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            188..622
                     /locus_tag="WJL20_000629"
     CDS             188..622
                     /locus_tag="WJL20_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7682809.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WJL20_000630"
     CDS             complement(671..2014)
                     /locus_tag="WJL20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7682810.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WJL20_000631"
     CDS             complement(2019..2672)
                     /locus_tag="WJL20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ7682811.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WJL20_000632"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WJL20_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ7682812.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WJL20_000633"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WJL20_000633"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ7682813.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WJL20_000634"
     CDS             4618..5550
                     /locus_tag="WJL20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ7682814.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WJL20_000635"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WJL20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ7682815.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WJL20_000636"
     CDS             7115..8125
                     /locus_tag="WJL20_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ7682816.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WJL20_000637"
     CDS             complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WJL20_000637"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ7682817.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9109..10158
                     /locus_tag="WJL20_000638"
     CDS             9109..10158
                     /locus_tag="WJL20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7682818.1"
                     /translation="MSKQPTSKRQWRDGAAPSTKKTAKPFKSKARPKDETGKTASQAY
                     GQKASDGIKPQNLPKQRTAKARKLVVRNPNQKIMERARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10227..11768)
                     /locus_tag="WJL20_000639"
     CDS             complement(10227..11768)
                     /locus_tag="WJL20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ7682819.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
     gene            complement(12379..13734)
                     /locus_tag="WJL20_000640"
     CDS             complement(12379..13734)
                     /locus_tag="WJL20_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ7682820.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13931..14752
                     /gene="nadE"
                     /locus_tag="WJL20_000641"
     CDS             13931..14752
                     /gene="nadE"
                     /locus_tag="WJL20_000641"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ7682821.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14822..15295)
                     /locus_tag="WJL20_000642"
     CDS             complement(14822..15295)
                     /locus_tag="WJL20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7682822.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            15593..15925
                     /gene="trxA"
                     /locus_tag="WJL20_000643"
     CDS             15593..15925
                     /gene="trxA"
                     /locus_tag="WJL20_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ7682823.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(15978..16925)
                     /locus_tag="WJL20_000644"
     CDS             complement(15978..16925)
                     /locus_tag="WJL20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7682824.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            17253..18641
                     /locus_tag="WJL20_000645"
     CDS             17253..18641
                     /locus_tag="WJL20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7682825.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPTDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
BASE COUNT         4695 a         5462 c         4754 g         3870 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt gcaagcgtag
     1141 tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga aacagcgtga
     1201 taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc atcgcatcgg
     1441 tatcttcaac cgccttgagc gtgcccgcct catccaacca cgcgcctgcc aaaggaatgc
     1501 cgtctgaaac caaatcaaac aacgcctgac tgacgcgtgc cacgctgggc gcgggtttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcaaacctt acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg cgagcagttc gggagcgagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcagg cagcgtccga
     1801 cttcttcgca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcagacg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccatg
     2101 atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt ccctgcaaca
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat cagcgcgatg
     2221 tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact atggtcgaac
     2941 agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcgggtg
     3061 ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctatat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cctacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca aaaacccatg
     3601 gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac ccgcatcgca
     3721 ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg agcgcgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg gcctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttgcc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccggcgtgg tggaatgtgc tttgccgggc gtactgccgg taatgaaccg tgaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggac
     5881 ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaaccgcgcc ggcacgcctt tattggaagt ggtgtccgaa
     6061 cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca aatcgagatt
     6301 ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgaccccgat
     6421 ttgctgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagcaagc
     6601 gggcaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc actgaacaaa
     6661 gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc gctggtaggc
     6721 aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aagccaccat tgccgaaatc atcgaaaaac acggtttgca acagatgacc
     6841 gacaccggcg agattgaagc catggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaag
     6961 gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc caaactggcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcatt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatgg gcggacactc cgaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc cgtttgcctt
     7501 ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactgcac atccgcagac gtgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcattgtccg
     7681 cgttaaaggc ccgctcggtt cgtttacctt gcaggaagac agcggcaagc ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 aggcagcagc cgcgtcgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcagaccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc agagagtgct ggcaaggaag aaaaggccac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ctatgaccga
     8041 acaggcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccgtgaaa aaatgccgtc tgaaacccga
     8221 ttcctgtttt cagacggcat atgttttttc ctgttcaagg cgacagccgc tcgcgtatcc
     8281 agccgccatc cagcaaacgg tattggatgc ggtcgtgcag cctgctcggc ctgccctgcc
     8341 aaaactcaat caaatcggga atcacaatat agccgcccca atgcggcgga cgcggcacgt
     8401 gcaaaggatg tttgagtccg accgccgccg cctttgccac caataccgcc ttgttcggaa
     8461 tcacttcgct ctgcgcgctt gcccacgcac ccaaacggct ctgatacggg cggctctcaa
     8521 aatattcgtc cgacaatttc tccgccagcc tttcaacacg cccttccacg cgcacctgac
     8581 gctccagctc cggccaaaaa aacgtcatcg ccgcaaatgg atgagcatcc agcgaacgcc
     8641 ccttgcggct gtgataattc gtaaagaaaa caaaaccttc agaattaact tccttcagca
     8701 gcaccatacg gctgttgggt ctgccgcgtc cgtcgaccgc cgccacatta accgccgtcg
     8761 gctcgttaac ctgtgcgcgt accgcctcgt ccaaccaccg ctcgaactgc tcgatcggat
     8821 tgtcggcgca atcggcttcc gacaattccc gtttgctgta atcttcccga atattgtgca
     8881 aatccatgtt accgctcctc ttgttatttg aaagattcta cccccgcacg caaaccgatt
     8941 tcaaccgttg cacaaacttt gtccccaaag ccacagcgac gatttcatcc acaaacacgc
     9001 cgcataaggt acaatatcga accgtccgac tgaggacggc attttatcaa cccgtcctgc
     9061 cgcacacgcc gcagaagaac cgccttatca ggcgagttag gaaaaatgat gtccaagcaa
     9121 cccaccagca aacgccaatg gcgcgacggt gcagccccgt ctaccaagaa aaccgccaaa
     9181 ccgttcaaaa gcaaagcccg tcccaaagac gaaacgggca aaaccgcatc ccaagcttac
     9241 ggacaaaaag cttcagatgg catcaagccg caaaacctcc ccaaacagcg caccgccaaa
     9301 gccagaaaac tcgtcgtccg caatcccaac caaaaaatca tggaacgcgc gcgcgatttg
     9361 aaagaacgcc gcagcgactt gtcgcgcatg gaacccgaac gcctgcaaaa agtgcttgca
     9421 gcctccggcg tcggctcgcg ccgcgaaatg gaagaatgga ttaacaacgg ctgggtaacg
     9481 gtcaacggca aaaccgcgca actgggcgac aaagttaccc ccgacgacca cgttaccgtc
     9541 aaaggcagca tcatcaagct caaatgggcg gatcgcctgc cgcgcatcat cctgtattac
     9601 aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag gcagggtcag catcttcgac
     9661 cgcctgccgc aggccgccag cagccgctgg gtcgccatcg gacgcttgga catcaacacc
     9721 agcggacttc tgattctcac cacctccggc gaacttgtcc aacggttcgc ccaccccagc
     9781 ttcgaagttg aacgcgaata cgccgtgcgc gtactgggcg ggctgaccgg cgaacaaatg
     9841 cgcgtcctca ccgaagaagg cgtaatgctc gaagacggct tggcaaaagt cgaacgcatc
     9901 tacgaacaag gcggcgaagg cgcgaacaaa tggtacaacg tcgtaattaa agaaggccgc
     9961 aaccgcgaag tgcgccgcat attcgaaagc caaggcctca ccgtcagccg cctcgtgcgc
    10021 gtcggcttcg gtcccatcgg actgcccaac cgcctcaaac gcgggcagtt ctacgaactc
    10081 aatcccgccg aagtcgccaa catcatcaaa tgggcggaca tgctgctgcc gggcgaacgc
    10141 cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc cgtctgaaac atctactgtt
    10201 tcagacggca ttttgttcgg gcgttttcag gagaaaaggt cgagtgcttt gacaaagatc
    10261 atcaccacgc cgtaggcgag cggtatgacg gccaatgccc aacgccacca gaccgatatg
    10321 ccgccgccgg aaactttttc gcccacaagg taagcgtcgg atatggcggt ttcgtcatcg
    10381 gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt ggtgtttttc gtgtacggat
    10441 ttgacggcga ggttgcacaa caaaccgata atcagcagac ccgccatgat gtacatggtt
    10501 acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt ggctttggcg tatgtaattg
    10561 accagtaccg ggccgatgac ggcggcggtt gaccaggcca gcaggatgcg cccgtgaatc
    10621 gcgccgacct gataggtgcc gaacaggtct ttcaggtagg cgggaatggc ggcaaatccg
    10681 ccgccgtaca tggaaataat cacgcaaaag ccgatgatga acagggcttt gctaccaccc
    10741 tcgccgatgg agggaacggc gaaatacagc agcgaaccga gtacgaagaa aatggtgtag
    10801 gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata aaaagcgtcc gcccatgtta
    10861 aacaggctaa ggaggctgac gaagcctgcc gccgcacctg caccgactgc cgcctgcctg
    10921 cctacggagg tttcggaaaa gagttcctga atcatcacgg atgcctgacc caatacgccg
    10981 atgccggcag ttacgttcag gcacaatacc cagaacaaca gccaaaactg cggcgttttc
    11041 atggcttggg acacgttgac atgattgctg ctgaccagct tgttttgcgt tttcggcgcg
    11101 gtatagcctt caggtttcca gccgtcggca ggtacgcgga tggtaaacgc gccaaacatc
    11161 atcagtgcga ggtaaagcag acccaatacg gcgaaggttt cggcaacccc aaccgaagca
    11221 gcgtttgaaa aggcgttcat cagtgatacg gaaagcggcg aggcaagcat tgcgccgccg
    11281 ccgaaaccca taatcgccaa accggtcgcc atacccggct tgtcgggaaa ccatttcatc
    11341 agtgtggaaa ccggcccgat gtagcccaaa cccaagccta cgccgccgat gacgccgttg
    11401 cccaaataga gcaggaagag gttgtgcgta cgcacgccga atgcggatac gaagaagccc
    11461 aagctgaagc agcaggcggc ggcaaatatg gctttgcgcg gccctacccg ttccatccac
    11521 gtaccgaaca gggcggccga cgcgcccagc atcgcgagtg cgatactgaa aatccaacct
    11581 acggtcgtca gcttccaatc tccggccgcc gattcggtta tgccgataag tttggtcagc
    11641 ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg caaggtgtac cgccaatgct
    11701 gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa tgcttgcctc acggtctaaa
    11761 aatttcataa catcctcttt ctgtcagttg aaaaataaaa tttcatttgc ccaatggaaa
    11821 cttattgaaa attataaaaa aatatcgggt cgggttttta tccgccccaa gatgcgccgt
    11881 ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc cgacaaattc ctgcggcttt
    11941 tcgcttccgg attcccgctt ttgcgggaat gacgaattaa agattatctt aaggtcaagg
    12001 gactggattc ccgcttttgc gggaatgacg gcggcgggtt gctgtttttt atttataaat
    12061 ttctgtaact ttggatttca gccatcttca ataaattatc gaaacgtatc aatcgtcatt
    12121 cccgcgcagg cgggaatcca tccagttcgt tcgatttcga ttttttaagt ttcgagtaac
    12181 ttctgactcg tcattcccgc gtaggcggga atccggtttt ttgagtttca gtcatttcta
    12241 atacattccc accacttttc gcttccggat tcccgctttt tcaggaatga cgaattaaag
    12301 attatcttaa ggtcaaggga ctggactccc gcttttgcgg gaatgacggc ggagggggag
    12361 cggtttttcc gattgggttt aaatgcaatc gaacaaatcc tgctgccctt gttctttgct
    12421 tacgcgcacg tcggtttcgc cgtcggcgaa ggtaatgtgc agcttctgcc cctgcttcaa
    12481 aacatcggcg ttgcggatga cttgtccgcg tgtgtttttg acgacggaaa agccgcgctc
    12541 cagaatgtgc tgcggcgaaa cggcttcgag caatgcggct tgggcggtca ggctttggcg
    12601 gcggtgggta agcagttggc tgaaggcggc gggcaatgcc gtctgaaggc ggacgatgtt
    12661 gtttttgcaa acggaaacat caggacggta atgttgcagg gcttgggttt ggcgttcaaa
    12721 acgggcggtg tgggcacgga ggttttgctt catagagtaa gacagcgttt gcgccagctt
    12781 gccgattgaa gcgcgctgct catcaagttt ttggcgcgga tgacggattt gccgcgcaag
    12841 ccagtcgagt ttttggctgg catcgaaata gcgttgttcc aaaacggttt tcagacggcc
    12901 ttgcgcctga gcgagacgat gtagcgattc ttggcggttg gggctgacca gctccgccgc
    12961 accggtcggc gtgggcgcgc gcacgtcggc gacaaaatcg gcaagcgtaa aatcggtttc
    13021 gtgtcccacg ccactgacga cgggaaccgt gcaggcttcg attgcgcgca cgaccggttc
    13081 ttcgttaaac gcccacaagt cttcaatgct gccgccgccg cgacagacaa tcaacacgtc
    13141 gcattcggcg cgttgcgagg cggttttaat cgcttgggca atttgtaact cgctgcctgc
    13201 gccttgaaca ggtgtcggat aaacgataac gggaatttcg ggcgcgcggc gtttcaaggt
    13261 ggtaacgaca tcgcgcaatg ccgctgctgc caggctggtg acgatgccga tacattgcgg
    13321 acgggtaggc aacggctttt tgcgttccgc cgcaaacgcg ccttccgcct gcaactgtgc
    13381 cttcaaccgc tcgtaggctt cgtaaagctg ccccaaacct ttgagccgca cctcattcac
    13441 ggtaatttga aattcgcccc gcgcctcgta aatactgatt tttcctgata cttcgatatg
    13501 gtcgccttct ttcaaaggct tcgccaaacg cgccgccgca cctttgaaca tcgcgcaacg
    13561 cacctgcgcg cggctgtctt tgagcgagaa ataataatgt ccgctggcgg cacgggtcag
    13621 gttggacact tcgcccgcaa tccacaggcc ggcaaggtgg ttttccaaaa gacttttggc
    13681 aaatgcgttc aactcggaaa cggacaacac gtcagaatga aaaaaatcag acatagaatc
    13741 aatcaaataa taaaaaatat gaatatgttt tgaagcctaa ggcggtatta gtccgcctaa
    13801 attgtcaaca atattataac acgcgccatc ttgccgcccg ccttttcccg tatgactttt
    13861 ttaagcgggg aatgggaaaa atattcatca acccgccctc aatctattca aattacaccg
    13921 ccgacaagct atgatgcgga tattttcgac aggaggaaaa atggatacgc aggcagttat
    13981 cacacatatt gtccgatggt tggacgaata cgccgcccgg gcaaatgcaa aagggtttgt
    14041 cgtgggcgtt tccggcggca tcgattccgc cgtcgtctcc gcactcgccg cccgcaccgg
    14101 ccgcaccacg ctgcttctgg atatgccgat acgccaacac cccgaccagc ttgagcgggc
    14161 aaggctgcac atccgcaatc tgcaacggca atatgccaat gtaagcgcac aaacggtcga
    14221 tctgaccgac accttccaga cctttgaaca aaccgtcggc gttcatcaga cggcatttga
    14281 caatcagccg ctttccctcg ccaacgccag aagccgcctg cgtatgctga ccctgtacta
    14341 ctacgggcag atacacggac tactggttac ggggacaggt aacaaaattg aagatttcgg
    14401 tgtaggattt tttaccaaat acggcgacgg cggcgtggac atcagcccga ttgccgacct
    14461 gaccaaaacc caggtttacc ggcttgccga agcattgggc gtggacgagg cgattcaaaa
    14521 agccccgccg accgacggcc tgtgggatac ggaacgcacc gacgaggaac agatgggcgc
    14581 gagctatccc gaactggagt gggcaatggg cgtgtacggc acgcgcaaac ccgaagattt
    14641 tgaagggcgg cagcgcgaag ttctggaaat ctatacgcgg ctgcaccgcg cgatgcagca
    14701 caaaatcaac ccgattcccg tgtgccgcat tccgcccgaa ttgttgggct gaaacgtgga
    14761 aatgccgtct gaaacggaaa accgtatttc agacggcatg gaaatatccg actcctatcc
    14821 cttaagaatc gagtacgcgg gcaaacaaaa tatcgttttc caaatgaatg tggtcgttca
    14881 aatcctccac catttctttc gccagcgcgt aaagccgcgt ccagcttccg caagcccctt
    14941 ctggcggctg gaaattgtcg gtcagctctt tgagccgtgc gatggcgcgg tcgtgttctt
    15001 catgttcgtg catcatcacg ccgatgggca tcgccgcacc gcgtccgaca ccctgattaa
    15061 tcatcggaaa cagcatccgc tcctccttca tcatatgcat cagcagttcg ttctgcatat
    15121 aggcaagcag cccggcaatt tctgccggaa aggtatcggc atggacttgg gccactttct
    15181 gcgccagcgg caccaattct tcaaattgtg cgcggtggac attgtggtag cgttgcagga
    15241 tatgatcgac ggttgcacca aagggggcgg tctcccaaac ggaaaaatca gtcatcgcag
    15301 tgttcctttt acagggtttc gggtttggtt ttgaacattc atactttaag aatcaattca
    15361 aacggagcat acaccgcccg cgcgcttctg tacagcctca aacgtattcc ttacattttg
    15421 ataataaaag taattttcag aaataaaata ctgtccgaac cgttttttag aatttgcaaa
    15481 ggcgattggg gcggtacaga aaaactatta tcccgcccgc ccacttgaaa tttttatgcc
    15541 caagccctat cctgcacgct atcgtgccaa tcccaaccga aaaggaaaaa taatgagcag
    15601 cgaattgatt gtacacacca gcgatgccgc ttttgaaaaa gacgttttaa atgcagatat
    15661 ccccgtcctg ctggactttt gggctccgtg gtgcggcccc tgcaaaatga ttgccccgat
    15721 tttggacgac attgccgccg aatttgaagg ccgtctgaaa gtggtcaaaa tcaacatcga
    15781 cgacaacgaa gccaccccgt cccgtttcgg cgtgcgcggc attccgaccc tgatggtgtt
    15841 caaaaacggc gaagtcgtcg ccaccaaagt cggcgcattg gcaaaaggtc agctgaccgc
    15901 ctttgtcgaa gcctctatcg cctgataaag cgcaatcgaa aaagccgccg gaagattccg
    15961 gcggcttttt cgcaccctta agatttgtgg cggatttccc agcacctatg gatttttttg
    16021 ttgcggaaat cttcgggaac ggattgtttg gaaatgtctt tgacggcgta ttgttccgat
    16081 accaagtcgt ctaagacgaa gctgcgcagg ttgttggaaa agtacaaaat gccgtctgaa
    16141 gcgagcagct tcaccgcgcc gtcaatcagc tttttgtggt cgcgctggat gtcgaggatg
    16201 tcggacattt tcttgctgtt ggaaaaactg ggcgggtcca tcacaatgag gtcgaaccgc
    16261 ctgccttccc catatgccgt ctgaagatat tggaacacgt cggcgcggac gattttatgc
    16321 cgttccgtat cgatgccgtt caattcaaaa ttgcgtctcg cccaatcgag ataagtgtta
    16381 gacaaatcga cagtttcgct ggatgccgcg ccgccggtgg cggcatagac agtgaagctg
    16441 ccggtgtagg aaaacaggtt taaaaaacgt ttgcccgccg ccgtttcgcc gacttttttg
    16501 cgcgtgtttc gatgatccaa aaaaagcccc gtatccaaat acttatcaag gttgacccaa
    16561 aacttgcggc cgttttcggt gatgacgaaa tcgtcgcccg ccttgccggt tttttcgtat
    16621 tgctgcaaac ctttttggcg ttcgcggcgt ttgaggcgga tttgttcggg cgcaaaaccg
    16681 gtaacgaagg cgacggcttc caagacttcg gcaagccacg cttcgtattc ttcgggccgc
    16741 atcagccagc cggtatcgta ttcctgaagg tggattcgat cgccgtaaac atcggcggca
    16801 aaggggaatt gggggatgtc gcggtcgtaa atgcgccagg cttcgatgcc gttgcgtttc
    16861 gcccatttca taaggtgttt gatgtttttg cccaagcggt tggcaaacgg tgtgatgtcg
    16921 gtcattggtt tcaggcggaa taaagtggaa aacggcaatt ttactgtaat taacgcccga
    16981 ttgcttgacc gtttcgggca aaccctatac catccgccgc ttatcttgtc atacgaagcc
    17041 atcgccttcc aacctaaacc gcccttacgg gcgcgtttct tttgttgctt tgattttgca
    17101 aagcctttct gtgcaggttg tcgtcgatgt aaaccacaag caagccgctt gcgacaaccc
    17161 tgtaacttca cattccccgt atcgttaccc ttccctgctt caggccgtct gaacctttcg
    17221 gacgcgggcg ttgttgtctt ccaaggatag ccatgtctat taaatttgcc gatttgaacc
    17281 ttgataaaaa cattttgtcc gccgtcagca gcgagggtta cgaaagcccg acgccgattc
    17341 aggcgcaggc gattccgttt gctttggaag gccgcgacat catggcttcg gcgcaaaccg
    17401 gctccggcaa aaccgccgcc tttctgttac cgactttgca gcgactgacc aaacgcagcg
    17461 aaaaaccggg caaaggcccg cgtgctttgg tgttgacccc gacccgcgaa ttggcggcac
    17521 aagtggaaaa aaacgcgctg gcgtatgcca aaaatatgcg ctggttccgt accgtcagca
    17581 tcgtcggcgg cgcatcgttc ggctaccaaa cccgcgccct gagcaaaccg gtcgatctga
    17641 ttgtcgccac gccgggccgc ctgatggacc tgatgcaaag cggcaaagtt gattttgaac
    17701 gtttggaagt gctgattttg gacgaagccg accgtatgtt ggatatgggc tttatcgacg
    17761 acatcgaaac catcgtcgaa gccaccccga ccgaccgtca gactttattg ttctccgcca
    17821 cttgggacgg cgcggtcggc aaactggcac gcaaactgac caaagaccct gaaatcatcg
    17881 aagtcgaacg cgtggacgac caaggcaaaa tcgaagaaca actgctgtac tgcgacgata
    17941 tgcgtcacaa aaaccgcctg ctcgaccaca tcctgcgcga tgccaatatc gatcaatgcg
    18001 tgattttcac gtccaccaaa gcgatgaccg aagtcatcgc ggatgaattg tacgaaaaag
    18061 gtttcgccgc caactgcctg cacggcgaca tgccgcaggg ctggcgcaac cgcacgctga
    18121 tggatttacg caaaggccgc tgcaaaattt tggttgccac cgacgttgcc gcacgcggta
    18181 tcgacgtacc gaccattacc cacgtcatca actacgacct gccgaaacag gcggaagact
    18241 acgtccaccg catcgggcgc accggccgcg caggccgcac gggtattgcg attacgtttg
    18301 ccgaagtgaa cgaatacgtc aaagtccaca aaatcgaaaa atacattaac cgcaaactgc
    18361 ccgaactgac catcgaaggc atggaaccga cccgcaaacg caaatccgca ggcggtaagc
    18421 caaaaggcaa aggcggctgg ggcgatagga aatccggcgg ccggcgcggc gatcataaac
    18481 cgggcaaaga aggcttcggc ggcaaaacgc gcggcgaagg tttcaagaaa gaaggcttta
    18541 agaaagacgg tttcaaaaaa accggcgaag gcttcaaagg caaacgtaaa gccggcgatt
    18601 cttttgcagg cagaggcgaa cgccgttaca aagaccgcta agccccaacc tgcctcataa
    18661 accaatgccg tctgaaaccg atttcgagtt tcagacggca tttttgcaat gtttcaacac
    18721 cgcccggctt tgatacccaa aggattaatc tgcaatatag tggattaaca aaaaccagta
    18781 c
//
LOCUS       DAWKQJ010000025        18617 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000025 DAWKQJ010000000
VERSION     DAWKQJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.025"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1586)
                     /locus_tag="WJL20_000646"
     CDS             complement(<1..1586)
                     /locus_tag="WJL20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ7682826.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSV"
     gene            complement(1693..2088)
                     /gene="hisI"
                     /locus_tag="WJL20_000647"
     CDS             complement(1693..2088)
                     /gene="hisI"
                     /locus_tag="WJL20_000647"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ7682827.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRALRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(2119..2886)
                     /gene="hisF"
                     /locus_tag="WJL20_000648"
     CDS             complement(2119..2886)
                     /gene="hisF"
                     /locus_tag="WJL20_000648"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ7682828.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGNPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(2899..3636)
                     /gene="hisA"
                     /locus_tag="WJL20_000649"
     CDS             complement(2899..3636)
                     /gene="hisA"
                     /locus_tag="WJL20_000649"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ7682829.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(3669..4307)
                     /gene="hisH"
                     /locus_tag="WJL20_000650"
     CDS             complement(3669..4307)
                     /gene="hisH"
                     /locus_tag="WJL20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ7682830.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVWTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(4438..5940)
                     /gene="pta"
                     /locus_tag="WJL20_000651"
     CDS             complement(4438..5940)
                     /gene="pta"
                     /locus_tag="WJL20_000651"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ7682831.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(6125..7183)
                     /gene="fbpC"
                     /locus_tag="WJL20_000652"
     CDS             complement(6125..7183)
                     /gene="fbpC"
                     /locus_tag="WJL20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ7682832.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGSALFFPGNTL"
     gene            complement(7204..8721)
                     /gene="fbpB"
                     /locus_tag="WJL20_000653"
     CDS             complement(7204..8721)
                     /gene="fbpB"
                     /locus_tag="WJL20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ7682833.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(8789..9784)
                     /gene="fbpA"
                     /locus_tag="WJL20_000654"
     CDS             complement(8789..9784)
                     /gene="fbpA"
                     /locus_tag="WJL20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ7682834.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(10035..10504)
                     /locus_tag="WJL20_000655"
                     /pseudo
     CDS             complement(10035..10504)
                     /locus_tag="WJL20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(10561..11937)
                     /gene="argH"
                     /locus_tag="WJL20_000656"
     CDS             complement(10561..11937)
                     /gene="argH"
                     /locus_tag="WJL20_000656"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ7682835.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(11956..12825)
                     /gene="galU"
                     /locus_tag="WJL20_000657"
     CDS             complement(11956..12825)
                     /gene="galU"
                     /locus_tag="WJL20_000657"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7682836.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(12853..13452)
                     /gene="rdgB"
                     /locus_tag="WJL20_000658"
     CDS             complement(12853..13452)
                     /gene="rdgB"
                     /locus_tag="WJL20_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ7682837.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(13445..13678)
                     /locus_tag="WJL20_000659"
     CDS             complement(13445..13678)
                     /locus_tag="WJL20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682838.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(13786..14319)
                     /locus_tag="WJL20_000660"
     CDS             complement(13786..14319)
                     /locus_tag="WJL20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ7682839.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(14421..14879)
                     /gene="nudB"
                     /locus_tag="WJL20_000661"
     CDS             complement(14421..14879)
                     /gene="nudB"
                     /locus_tag="WJL20_000661"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ7682840.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            15090..15166
                     /locus_tag="WJL20_000662"
     tRNA            15090..15166
                     /locus_tag="WJL20_000662"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15124..15126,aa:Pro,seq:ggg)
     gene            15324..17723
                     /gene="mafB"
                     /locus_tag="WJL20_000663"
     CDS             15324..17723
                     /gene="mafB"
                     /locus_tag="WJL20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ7682841.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYSAGNLSK
                     KVLTLVYINGKLKFGPSSLPSSALKAPVPIVGKAEAPPGKAVPVGEARIPSNPKAVKG
                     TTVTLSQEQRLANGELIPKGSVVTWSDNAVKVVRPDGKKRRFNSRDWEQKALPKPQLP
                     KDYRDDINIGGNNPRFNQQRHEKIESHNPNRIEEIRQRNAVSSAERQADALKRMQTSG
                     RTRLPGQPQKGKPGAAREMSSSDNPNQTAEAFARRYLGRKPTLMERVEGEKLYGGKCV
                     GCWKAQMPDGTFVVYRPAGNASMTKGTTASVDINNPTLNNLNINLKGKPTELKFKFPA
                     R"
     gene            17734..18195
                     /locus_tag="WJL20_000664"
     CDS             17734..18195
                     /locus_tag="WJL20_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682842.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            18221..18469
                     /locus_tag="WJL20_000665"
     CDS             18221..18469
                     /locus_tag="WJL20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7682843.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
BASE COUNT         4075 a         4499 c         5530 g         4513 t
ORIGIN      
        1 accgaatgct cgcgcgtttc gtggaacacg atgtccggcc agcgttcttg cgtgagtccc
       61 aaattcacgc ggttgggggc gaggtaggcg aggttgccgc ccgcgtcgat ggcgaggttg
      121 cccgcgttgg ctttttcaaa ttccgccagt tttttcttgt cgtcgcacga tacccagcgc
      181 gccgaccaga tggatgcgct gtcgaacacg gcttccacgc cgtattcgtt ggcaaggcgc
      241 gaggtaacga cttcaaactg caacacgccg accgcgccca aaatcaaatc cgcgccgctc
      301 atcggtttga acacctgcac cgcgccttct tcgccgagct gttgcaagcc tttttgcagt
      361 tgtttgattt tcagcgggtt tttgatgcgt acgctgcgga acagttcggg tgcgaagaat
      421 gggatgccgg tgaacgccag ttgttcgcct tcggagaagc tgtcgccgat ttggatgttg
      481 ccgtggttcg ggatgccgat aatgtcgccg gcgtaggctt cttcaaccag ctcgcggtcg
      541 tgcgacatga aggtaaccac gctggaggcg gcgatttcgc ggttgatacg caggtgtttc
      601 atcttcatgc cgcgctcgaa tttgccggag cagacgcgca agaaggcaat acggtcgcgg
      661 tgtttcgggt ccatattggc ttggattttg aagataaatc cggaaaactt cggctcgtcc
      721 ggctcgacca tacgtacggt cgcgtcgcgc ggtttcggcg cgggcgccca gtcaatcaat
      781 gaattgagga tttcctgaat accgaagttg ttaatcgcag agccgaagaa tacgggcgtg
      841 agttcgccgg cgaggaattc gtcgagatta aactcgttgg aagccgcctg caccaattcg
      901 atttcgtcgc gcaactgctg gatttccaac ggaaagcgtt gttccaattc aggattatcg
      961 atgcctttga tgatgtcgaa ctcgtgcggc aggcgttcgc cgccagcttc aaagagataa
     1021 atttcatcgt tcaggatgtg gtacacgccc ttgaagtttt tgcccatacc gatcggccag
     1081 gtaacgggcg cgcagcggat ttttaaaatg ttttccactt cgtccaaaag ttccagggaa
     1141 tcgcgcactt cgcggtcgta tttgttcata aacgtaacaa tcggtgtatc gcgcaggcgg
     1201 cagacgttta agagcttgat ggtttgcgct tccacgcctt ttgccgcgtc gatgaccatt
     1261 aatgcgctgt ccacggcggt taaaacgcgg taggtgtctt cggagaagtc ttggtgtccc
     1321 ggcgtgtcca agaggttgac ggtgtggtct ttgtaatcga actgcatcac acttgatgcc
     1381 acggaaatgc cgcgctgctt ctcgatgtcc atccagtcgg aggtggcgaa tttgccggtt
     1441 ttcttgcctt ttaccgtacc cgcgctctga atcgcgcccg aaaacagcaa gagtttttcg
     1501 gtcagcgtgg ttttacccgc gtcagggtgg gagatgatgg caaacgtgcg gcggcggcgc
     1561 acttggtcga ggatttcttg ggacatggtt ttctttgcaa aaaggttcag gccgcttttc
     1621 agacggcccg gacagtgttt gagacggcga gattgtacaa aaaaatgcct gataattcaa
     1681 tgttggaggc ggtcagtgcg tgctgccgta aatctctttt tcgtctttca ggacggcatc
     1741 ggcggtttcc cacgcgccgc cgcgccagac tttgtaaaag cagctttctc gcccggtgtg
     1801 gcaggcgatg ccgccgtttt gggcgatgag catcacaatg gcgtcgccgt cgcagtcgag
     1861 gcgcagtgcg cggacttttt gcgtgtgtcc cgactcttcg cccttcatcc attgtttttg
     1921 gcgcgaacgg ctgtaatagt gggcaaagcc ggtttcgacg gttttttgca gggcttcggc
     1981 gttcatccac gctaccatta aaacacgttt ggtttcggca tcttgggcga tggcgcaaac
     2041 caaacctttt tcgtcaaatt tgacggcttc aagcaggttt ttatccatat ttcctttcag
     2101 acggcatagt cggggcggtc agaggcgcac ttcgatgccg gcttcgcgca tagcgcgttt
     2161 ggcttcgcgg atggcgattt ccccgaaatg gaaaatgccg gcggcaagta cggcatcggc
     2221 tttgccttcg gttatgcctt caatcaggtg ccggacattg ccgaccccgc cggaggcgat
     2281 gacggggata tcgacggctt cggcaacggc gcgggtcagc ggcaggttga aaccctgttt
     2341 cgtaccgtcc ctgtccatac cggtgagcag gatttcgccc gcgccgcgtt tttgcatttc
     2401 gaccgcccat tccaccgcat ccaaaccggt cggattgcgc ccgccgtggg taaagatttc
     2461 ccagcgtgtg ttttcggggt tgacggcttt ggcatcgacg gcggcgacga tggcttgcga
     2521 accgaaaaat ccggcggctt cgtcaatcag gtcgggacgg gtaacggcgg cggtgttgat
     2581 gctgactttg tccgcgccgg cattgagcag gcggcggatg tcggcaacgg tgcgtacgcc
     2641 gccgccgacg gtcaggggga tgaagacttg tccggcaacc tcttcgatga tgtgcaggat
     2701 ggtgtcgcgg ttgtcggatg aggcggtgat gtcgaggaag gtcaattcgt ccgcgccttc
     2761 gccgttgtag cgtttggcgg cttcgacggg gttgcccgcg tcgcgcaaac cgatgaagtt
     2821 cacgcctttg acgacgcgcc cgtcttttac gtcgagacag gggatgatgc gttttgccag
     2881 tgccataatc ggatgccttt agtcgaggga atctgccagt tgctgcgctt gggcaaaatc
     2941 gatgctgccc tcgtaaatcg cgcggccggt aatcgcgcct gctacgccat gtttttcggc
     3001 ggcacacagg gcgcggatgt cgtccaagcc ggtcagtccg ccggaggcga tgacgggaat
     3061 gcggacggct tgggcgagtt tgaccgtcgc gtcgatgttc acgccgctca tcataccgtc
     3121 gcgcccgatg tcggtgtaga tgatgctgtt gacgccgtcg tcttcaaagc gttttgccaa
     3181 atcaattaca tgatgcccgg ttacggttgc ccagccgtcg atggcggcca taccgtcttt
     3241 ggcatccagc ccgacgataa tcctgccggg gaaggctttg cacgcctcgc gcacgaagtc
     3301 gggatttttg actgccgccg tgccgataat cacgtcgttt aagcccaaat ccaaatattg
     3361 tccgatggtt ttcaaatcgc gtatgccgcc gccgagctgt acggggatgt ctttggcgac
     3421 ggcggcaagg atgtctttga tggcgggcag gttttgcgga acaccggcaa acgcgccgtt
     3481 caaatctacc agatgcaggc ggcgcgcgcc ttgttcgaac cagtgcagcg cggtttcggc
     3541 gggcgaatcg gaaaagacgg tcgcctcttc catcagccct tgtttcaggc ggacgcagcg
     3601 tccttctttc aaatcgatgg cgggtatcag cagcataatt tttctccttg tgcggggccg
     3661 tgtccggctt accagtttaa aaagtttttc aacatcgtca gcccggcatc gtggcttttt
     3721 tcggtgtgaa attgcgtggc gaatacgttg tctttgccga cgatgcaggc aaacggggac
     3781 gggtagtcgc tttcgcccaa tatggtttcg ggattttcgg gggcgaaata gtagctgtgg
     3841 acgaagtaaa aacgcgtgtc ttgggggatg ccttgaaaaa gcgggtggtt ttgggtttgg
     3901 cgcacggtgt tccagcccat atgcgggact ttcagacggc atccctgccg gtcgcggagg
     3961 tcgcgctcaa agcgtctgac tttgccgccg aaccagccca agccgtcggt gtttccttct
     4021 tcgctgtggt cgaataaaag ttgcgcgccg acgcagattc cgaaaaaggg tttgtttttt
     4081 aaggcatctt tgactgcctc gtccaagccg ccgcgcgtca gtgccgcaat acagtcgggc
     4141 atcgcgccct gcccgggaaa aatgactttg tcggcgcggg acacgcgttc ggggtcgccg
     4201 cttaaaaaga tttcggtatt ttttccggca agctgccccg ccgtccagac ggatttcaat
     4261 acggaatgca ggttgcccat accgtaatcg ataatggcga tttgcatggc ttcctcctct
     4321 ttttttgcaa tatggctgcg attttaacaa acaaatgtgc cgtgctgata aaaatgccgt
     4381 ctgaaaacgg gagtctgtct tcagacggca tagggtttaa acccggaaag ccgtttgtca
     4441 gccttccatt tgttttgcct gaacggcagt cagggcgatg gtgaacacga tgtcttccac
     4501 cagcgcgccg cgcgacaggt cgttgaccgg tttgcgcagg ccttgcagca gcgggccgac
     4561 gcttaagacg ttggcactgc gttggacggc tttataggtg cagttgccgg tgttcaggtc
     4621 ggggaagacc aaaacggttg cctgtcctgc caccgggctg cccggagctt tggatttgcc
     4681 cacgcccggc acggttgccg catcatattg cagcgggccg tcgatggcga ggtcggggcg
     4741 tttttcccgg gcaagtttgg ttgcttcgat gacggtatcg acatcggggc cgctgccgga
     4801 gttgacggtg gagtaggaaa tcatcgccac tttcgggtcg atgccgaagg cttttgcgga
     4861 atcggcagac tggatggcga tgtcggcaag ctgttgcgcg gtcgggttcg gattaaccgc
     4921 gcagtcgccg aagacgagga cttggttggg cagcagcata aagaatacgc tggacacgag
     4981 gcttgcgccc ggtgcggttt taatcagttg caaagcgggg cggatggtgt tggcggtggt
     5041 gtgaaccgca ccggatacca aaccgtccac atcattttgc gccatcatca tcgtaccgag
     5101 taccacggtg tcttgcagtt gcttgcgcgc gtcttcgggt gtcaggcctt tggatttgcg
     5161 cagttcgcac atcggtccga cgtattgttc gaccaatgag gcgggatcga tgatttccaa
     5221 agagtcgggc aggctgatac cgcgttcttt ggcaacggct tcgacttctt cgcgtttggc
     5281 aagcaggacg cagcgggcaa tgcctttttc gtggcagatg gcggcggctt ggacggtgcg
     5341 gggttctgcg ccttcaggca ggacgatgcg tttgtcggct tggcgggcga agtcgatcag
     5401 gttgtagcgg aattgcgccg gcgacaggcg ttttgcttcg cggcctgcca atacggatac
     5461 gtctttcagc gcgtcgctcg aaccgaagaa ggtcaggccg gttttttcgg ctgccgcttc
     5521 ggcaacggag gctgccgcgc cgtccacgac aaaaccttcc aatacgcccg gcgcggcggc
     5581 gaagaactgt ttggcaaggt tcagccgatt tgccagttcg tcggcatcgg cgttgtcgga
     5641 acggacggcg aagacggctg ccgcgtcaag ggacaatgcc agttcgacgt ttttgcctgc
     5701 gaggtagatt ttgtcggcat cgggcgcgat gccttcgatg acgaggttgg cggcatcgag
     5761 tgcggcaact ttgccgacca gtgcgtcgaa ccagtcgtcg cttttgcctt gcgcgagcag
     5821 ggtttcggcg gttgcgtcaa cggcttggaa aatttgtgcg tccagtgctt ttgcaaaggc
     5881 ttgtgcggcg gcggaggcgt ccagtccggc agatacgggt acgatgagta cttttgccat
     5941 gatatatcct ttcgtatgct gcggtgtgcg gcatatgtgg ttggaagggg cggcatatag
     6001 gcagaaacgg ctgcctgcgt gccgtgcgtg ccgtgtttgg cttgaggcgc gcaggttgaa
     6061 tatagcaaac aaattctgtt ttcaacaaga taaatatccg caggcttgtg gatgctgccg
     6121 cctttcagag ggtatttccg gggaagaaca gggcggaacc gtccaaatgg aggacggcgg
     6181 aaatgccgtc tgacagggtg ggggcggaag ggaggttgag cgtgaggacg gtttgtccga
     6241 cccggaggct gatttcggta tgccgcgctt tgggcgtggt tttgagaacc acggcgtgaa
     6301 tggaggcggt gggtgcggaa tgggggtgaa ggctgaactg ttccggacgg atgagcagtg
     6361 tgccgcgtgt gcctgcgggt gcgccgcttt gaacgggcag gcggcccaat ccgcaatcgg
     6421 cggtgccgtc ggcgttgagc gcggcgggga acacgatgcc ttcgccgata aacagggcgg
     6481 catcaaggtc ggcaggttgt cggtacaatt cgtgagggct tgcagtttgg aggatgcgcc
     6541 cctgtttcat cacggcaatc cggtcggcgt attgcagggc ttcttcgcgg tcgtggctga
     6601 cgaaaacggc agatttgccg ttggcgcgca gggcggcaat catgtcttcg cgaatctggc
     6661 ggcgcaactg ttcgtccagc gcgctgaagg gttcgtccaa caaaatcagt tcgggatcgg
     6721 gtgcgagggc gcgggcgagg gcgacgcgct gttgctgtcc gcccgaaagt tcgtgcggat
     6781 agcgtccggc aagttcggaa atgccggtca attccaacat agcttcgatg cgctgccgct
     6841 cttgcgccgt cttgcctttg ccgttgccca gcccgtaggc ggtgttgcgg taaacggtca
     6901 ggtgggggaa cagcacacct tcctgtacga cataacccaa acggcgttcg cggacgggga
     6961 ggttggtatt tttcgagaag atggttctgc cggaaagcga aatttcgcca aaatcgggtt
     7021 gttcaaaacc ggcaaggcag cgtaaaaggg tggttttgcc gcagccggac gcgccgacga
     7081 taaagaggat ttcgcccggg tcgaggctga gcgaaatgtc gtttaaaact ggggtgtttt
     7141 gaaaactttt ggacaggtgt ccgatgtgca gggcggcggt catggcggta cttcctcaag
     7201 ctgttatttg aaggcgtatt tcttcagcag gaatacgggg atgccggaaa ataataccag
     7261 catcagcgcg taaggggtgg cggcggcgta ttgtgcgtcc gatgtgtatt cccaaacggc
     7321 ggtggagagt gtgtggacat cgtcggtggt cagcagcagg gtggcggtca gctctttcat
     7381 cagtttgagg aagacgagtg cgaatgcggc ggtaatgccg ggcaggatgg acggcagtac
     7441 caacgtcctg aaaataaaga agtgtccgcg ccccaatgtt gcgccgacct gttccatccc
     7501 tttggggagt tgttccaagg aagtcctcag ggtggtttgc gccatcggca ggtaaagcat
     7561 gaaataggca aggatgacga cgataaaggt ttggtaaacg gcaggggtgt agttgatgct
     7621 gaaataaacc aaggataggg cgataaccaa accggggacg gcgtgcagta aaaacggcag
     7681 cctgtctatc caaacggtta aaaaattgcg atagcgaacc gatgcccaaa caaggggcaa
     7741 ggcacataat atagtcaaaa tcgcacctaa agccgatacg cttaaggaac ggataaaggc
     7801 atcaaatacg gatacgagcg cgaatgtgcc ggaagtgccg accatcatcc aatgtatcaa
     7861 tacgccaaag gggataataa tgcccaaagt caacaagctg cttaaaaaaa caatcgcgcc
     7921 gatctgaccg ggcagtttga gggttttgac gggataagga cgggcaacgc ctttgccgct
     7981 gtggtaaatc ttggctttgc cgcgaaatat gctttctcca aatacgacga tgccgcacac
     8041 cgccattaaa acagcggaaa gcagggcggc ggtattgttg ttgtaggaca tttcgtattc
     8101 ttggaaaatg gcggtggtaa aagtggggta gttcaaaata gataccgcgc caaattcgac
     8161 cagcatatgc agggcaatca gtaacacgct gctgccgatg gcgggtttga gctgggggag
     8221 gatggcggaa aaaaaggttt gcaggcggct tttgcccaag gacaggctga cttcttcgta
     8281 agacaggctg atgcgtttga gtgccgcctc gacgggcagg taggcgagcg ggaacgagga
     8341 caggctcata atcatcactg tcccccaaaa gccttcgaca cggaaggtca ggctgatcca
     8401 ggtgaaacag ctgacaaatg cggggatgca caaaggcagg gtgattgccg tctgaaaaaa
     8461 ggttttgccg aagaagcggt aacgttggaa caaaagggcg caggcaatgc ccaaaacaat
     8521 ggaaatcagg gtaacgcccg ccatcatcgt caaggtgttg gagagcaaat cccacatacg
     8581 cgggcggaac aacagttcga cggcgcggtt gatgccgacc tgccacgaac gcatagcgac
     8641 atataaaaaa ggcagggtaa gcggtagggc aatcagtagg atgaggccgg taagccaaat
     8701 gggtattttt ttaggagaca tagtgttttt tatcggcaaa acgggcggac agtataaatg
     8761 tccacccgtt tgacaatccg aaaacggctt atttcatacc ggcttgctca agcagccggg
     8821 tggcgtgttc tttttcggaa acagtggtgg cggacacttg gggtgcttcc aacttggcga
     8881 tgggttccaa attgaaagtg gataccacgt gcggattcaa aggatactcg gcacggacgg
     8941 cggtcagggc gcgctgtcct tccttgctgg cgaggaaggc gacgaatttt ttcgcctcat
     9001 ccttgttttg ggaggatttt aacacggctg cgccggaata ggtaacgagt gcgccgggat
     9061 ctctgtggcg gacgaaattc aggcgggtgt ggacattttg tacgcctttt tcacgcgcaa
     9121 aagcgtgcca gtagtagttg ttgatgaggg cggcatcgat ttcgccgttt tcaaccgctt
     9181 gaagggcgac ggagttttta gcgtaaggct tgccgtattc tttcagacct ttgagccatt
     9241 tcaatgcggc cgcttcgcct ttcagtttga cgatggcgac aacctgttcc aagaacgcgc
     9301 cggaagtggg ggcgtaaccg atgcggtttt tccatttcgg cgtggcgtaa ttcaggacgg
     9361 atttttccaa atctttttca gacagtttgc gggtgtcgta aacgacgacg cgcgaacgtc
     9421 cgctcagtgc cacccagtct tttttggcgg caaccggcac gcccttgccg cgtgtttcgt
     9481 tgatggtgga ggcgggcagg ggctctagga ggttggctgc ggaaagggtg gcgagtgccg
     9541 ggatttgttc ggaatagaat acgtcggcgg ggcttcggct gccttcttct ttgatttgac
     9601 cggcaagctg gtcgcctttg gcgctgttga gtttgacttt gatgccggta gcccgggtaa
     9661 aggcatctgc aacggcttgt gccgcttctt tgtgttggcc gttgtacacg gtaatgtctg
     9721 ccagcgcggg ggttgcggcg gtcagggctg cggcaagcag tgcgtatcgg atagatgttt
     9781 tcatatcgat tttctcctaa tgaatgagag tgtacacctt gttaagacat aacggcgtgt
     9841 agtgtattcc ttcttcttta taaatgcaaa taattatttt ttaaatttgt tattatccga
     9901 tccggttatt gtttgttctg acttgtattt tttccgtgca tcgcgcccgt aaggcggaag
     9961 cggcgggcaa tgcgtggcgg aatgtgggta aaggcggcat tttgatttgt cggaatgctt
    10021 gagaacccct ctctttaaaa cagcgtctga acagggttgc cggaatagta ttgccatccc
    10081 ggcagataca gtttgtcggg gtcttgccaa tattgttcat ccagactgtt cggcagtgag
    10141 gcggttttgt tgtcgagatg ttgcgttata tcccacattt ctttcaggtt ttaccttccg
    10201 attggaggtg gcggatttct tttcccaatg cgtctgccgc ccgatggatc agcattgcgc
    10261 tgtgtaccgc gccgattttt ttcagaacgg aacaagtctt ttcggcggcg gggaaactcc
    10321 agttgcagac aaattgcagt atgccgccgt tgccgatatc ggcttctgcc cgccaaatta
    10381 aaaccagctc ctgttcccac ccgtccatgc tttcgagttt gccgtcatgc tgttcaaaaa
    10441 gtttgtccac cgcctgccac atcatctgtt cgaagcggtc ggcttgggta tccgtatcgg
    10501 tcatagtgtt cttgtctttt aaaaatgccg tctgaacatt tcttcagacg gcatcggggg
    10561 ttaagctgac atttcccgcc agcgtttgac ttggaaacgg acctgttcag gcgcggtacc
    10621 gcccaagtgg ttgcgcgcgt ttaagctgcc ttcgggtgtc agcacgccgt aaacgtcgtc
    10681 ggcaatcaaa tcgctgaaac cttgtaagac ttcgagcggc agtttgctca aatcgacacc
    10741 cgcttggtcg gcgtggcgca cggcttgggc gacgacttcg tgggcatcgc ggaaaggcat
    10801 gccttttttt accaagtaat ctgccaaatc ggtggcggta gcgaagccct gcatcacggc
    10861 ggcgcgcata ttgtcgggtt tgacggttac gccgcgcatc atatcggcgt aaatccgcaa
    10921 cgtgtcgata agcgtgtcgg cggtgtcgaa caagggttct ttgtcttcct gattgtcttt
    10981 gttgtacgcc aagggctgcg atttcatcag ggtaatcaag ccgataaggt gtccgatgac
    11041 tcggccggat ttgccgcgca cgagttcggg cacgtcgggg tttttcttct gcggcatgat
    11101 ggacgaacct gtgcagaaac ggtcggcgat gtcgataaag ccgaaacgcg gactcatcca
    11161 caaaatcaat tcttcagaca ggcggctcag gtgaaccata accagcgagg cggcggcggt
    11221 gaactcgatg gcaaaatcgc ggtcggatac ggcatcgagc gagttttggc agatttgttc
    11281 aaagcccaac agtttggcgg tgatttcgcg ccgaatcgga taagtcgtac cggcaagggc
    11341 ggctgcgccg agcggcatac ggttgacgcg gcggcggcag tccgccatcc gttcgttatc
    11401 gcgtccgagc atttcgacgt aggcgagcat atggtgtccg aagctgacgg gctgggcgac
    11461 ttgcaggtgg gtaaagcctg gcatgacggt ttcggcgttt tgttccgcca aatccagcaa
    11521 tgccgtctga aggctttgaa tcaggcttcg gatgacggta atctggtcgc gcagccaaag
    11581 gcggatgtcg gtggcgactt ggtcgttgcg gctgcggccg gtgtgcaggc gtttgcccgc
    11641 gtcgccgatt ttgtcggtca ggcggcgttc gatgttcatg tggacatctt ccaaatcgga
    11701 cgaccattcg attttgccgc tgcggatttc ttcgaggatt tccgccatgc cccgacggat
    11761 gtccgccaaa tcgccttcgt ccaacacgcc ggtttctttc aacatttgcg cgtgtgccag
    11821 cgagccttga atgtcccatt cggcaagccg tcggtcgaaa ccgatggagg cggtgtattg
    11881 tttgacgagt tcggaaacgg gttcgttgaa acgtccggac caggttttgt cgtgcataag
    11941 gattccttga tggggttatt cggtgcggta tttttccaaa aggcggcgga agggttcgcc
    12001 ggtttcgggg tgtttcaggc cgtaggcgac ggtggcttcg aggtagccca gtttgctgcc
    12061 gcagtcgtag cgcgtgcctt caaaggggtg cgccaggaca aattcgtgat cgagcagctt
    12121 ggcgatgccg tctgtaagct ggatttcgtt gcccgcgccg cgcggcagtc cggtcagtaa
    12181 gtcgaaaatg cgcggggtaa ggatgtagcg tccgacgacg gcaaggttgg agggcgcgtc
    12241 ttcgggcttg ggtttttcga caatgccgat aatgcgttgg aactgtttga gctgttcggt
    12301 ttcgacgatg ccgtatgagc cggtttgcga tgcttcaacg gtttctacgc ccaaaatgct
    12361 gttgccgctg cgcccgtaca cttcgaccat ttgtttgagc gcgcctttgg gggcatcaat
    12421 caggtcgtcg gcaaggataa cggcaaaggg ttcgtctccg atggcggcgc gggcgcacaa
    12481 gacggcgtgt cccaagccca gtgcttccgc ctgacggatg tagaggcagg taatgttcgg
    12541 cggcaggatg ttgcggacgt gttccaacaa tttgtcttta tggcgcattt ccaactcggt
    12601 ttcgagttcg tatgccttgt cgaaatggtc ttcgatgctg cgtttgttgc gtccggtaac
    12661 aaacaccatt tccgtgcagc cggcttccac ggcttcttct acggcgtatt ggatcagcgg
    12721 cttgtcgacg atgggcagca tttctttcgg gcttgccttg gtggcgggca ggaagcgtgt
    12781 tcccatccct gcgacgggga aaacggcttt ccgtatgggt ttcattcttt ttcctttgta
    12841 ttgttttgat gtttaaaggg cgagtttgcg taagagttcg gcaagtgcct gcgcgcggtg
    12901 gctttcgcgg tttttgacct ccgaatccaa ttcggcggcg gttttgccgt gtccgggcag
    12961 ataaaaatac gggtcgtaac cgaaaccgtt ttcaccggcg ggaacgccgt tccattgtcc
    13021 gtgccatacg ccttcggcaa taatcggacg cgggtcgtct ttatggcgga caaaaaccaa
    13081 tacgcagacg tagcaacagc ttttgtcggc tttgccggca agttcggcgg caagtttcag
    13141 gttgttggcg gtatcggatt tgggattgct gccggcgtaa cgtgcggaat gtatgcccgg
    13201 agcaccgttt aaggcggcgg cacagatgcc gctgtcgtcg gcgagtgcgg gcagcccgct
    13261 gtgtccggcg gcgtgccggg cttttgccag cgcattttca acaaaggtgg aatagggttc
    13321 ggggcattcg ggtatgctga actcggattg cggcaatacg gtaatgccgt atggttcgaa
    13381 taggttgccg aattctttga gtttgcctgc attgccgctt gccaaaacga ttttttccgg
    13441 tttttcagac atagcggttt tcctttgcgg cggattgggc ggcgcgtagg gatttgtgcc
    13501 gcaggtagag ggcgaggctg ccgatttgtc cgaacagtgc gccgaatgcg aaaagaaagg
    13561 aggcgacggc gaaggctttg ctgccgacgg tcagcagata agcacccaag aggacgagca
    13621 gcataaatgc cagtgtgaag agggcaacag acaacagata gatttttcgg cggttcatgg
    13681 cgttcggtcg gaaacggtat gttcggatta tagccgattg ggacggtatt ccctagagct
    13741 tggaaaaatg ccgtctgaaa cggcgttcag acggcatggc gggcgttatg cctgtttgtt
    13801 ccaacgttcg atggattctt tgatgacttt tttcgcttct tccgcatcgc cccacgattc
    13861 gactttggtc gtacctgctt ttttcaggtc tttgtaatgg ttgaagtgga actcgatttg
    13921 tttgatgagc tgttgcggca aatcggacaa agttttgtag gcgttgccgt tgttgcggtc
    13981 gtcggcagga acgcagacga ttttgtcgtc tacttcgccg tcgtcaacga atttcatcac
    14041 gccgataacg cgcgcttcca agaatacgcc ggttgccaaa ggttgttcgg taacgagcag
    14101 cacgtccaat tcgtcgccgt cttcgtccaa agtttgggga atgaagccgt agttggtcgg
    14161 tttggcgaag atggcgggtt cgacgcggtc gagttggaat gcggcgagtt tgcggttcca
    14221 ctcgattttg tggttgctgc cggcggggat ttcgttgacg acgttgatga tgccgccgtc
    14281 cacatcgccg ggggtcagga tttggttgaa gtctgccatt tggtttcctt tgtttgggaa
    14341 agtttgaagt ttgaaagtat agcacaaacg tccggctgaa aatgcgcccg atgccgtctg
    14401 aaagggtgta cgggcgcgtg ttaccgtttg cccaaaaacc tgcccagttc cagaatcgcg
    14461 cgcctgttgg acggggaaaa cactttttcc gccgcttctt ccaagccgaa ccagccgtag
    14521 gagacgtgtt cctcgggttg caggacgacg ggcgtatcac gcgggatttc ggcagagaag
    14581 aggtgttcgc ggttttcaaa cacgcctttt ggatagcggt gccgccagtg gtggtagatt
    14641 tcgtaaaccg tgctgtcgtg ccagtcttga agctgcccgt ccgccagcag gatgccggtt
    14701 tcttcccaaa cttcgcgcct tgccgtttgt gcgacggttt cgcccggttc gaggctgccg
    14761 gttaccgact gccaaaatcc ttccggatgc gtgcgttcga tgagcaggat gccgccgtcc
    14821 ccgctataaa ggacgaccag tgcggaaacg gggtatttga gcggttttgc catcggcatc
    14881 tttcggcggg ctgcggtaat gaaggggcgg attatagcaa acgccgcacg ttatggcgtt
    14941 tatccttttc cgtatccttt tttctgcacg gatgggacgc gccggtgttt gccggtaaat
    15001 tttccgttgt gtcaaaaaga taagggcggt tgtgatttta atgcttgcca aagcgtcggg
    15061 cggaaactat aatccgcaac ttatcgagtc ggagtgtggc gcagtctggt agcgcacttg
    15121 catggggtgc aaggggtcga aggttcgaat cctttcactc cgaccaaaaa ttccgaaagc
    15181 cgctttcaaa agcggctttt ttgccgtccg tatgattatg atgtagagta cgcggcgaca
    15241 gacattcaaa tgccgtctga aaaccgttca gatggcatct ctttatctta gtttcattcc
    15301 gtaccatctt aaggaacatc aaattgggca tttcccgcaa aatatccctt attctgtcca
    15361 tactggcagt gtgcctgccg atgcatgcac acgcctcaga tttggcaaac gattctttta
    15421 tccggcaggt tctcgaccgt cagcatttcg aacccgacgg gaaataccac ctattcggca
    15481 gcagggggga acttgccgag cgcagcggcc atatcggatt gggaaacata caaagccatc
    15541 agttgggcaa cctgatgatt caacaggcgg ccattaaagg aaatatcggc tacattgtcc
    15601 gcttttccga tcacgggcac gaagtccatt cccccttcga caaccatgcc tcacattccg
    15661 attctgatga agccggtagt cccgttgacg gattcagcct ttaccgcatc cattgggacg
    15721 gatacgaaca ccatcccgcc gacggctatg acgggccaca gggcggcggc tatcccgctc
    15781 ccaaaggcgc gagggatata tacagctacg acataaaagg cgttgcccaa aatatccgcc
    15841 tcaacctgac cgacaaccgc agcaccggac aacggcttgt cgaccgtttc cacaataccg
    15901 gtagtatgct gacgcaagga gtaggcgacg gattcaaacg cgccacccga tacagccccg
    15961 agctggacag atcgggcaat gccgccgaag ctttcaacgg cactgcagat atcgtcaaaa
    16021 acatcatcgg cgcggcagga gaaattgttg gcgcaggcga tgccgtgcag ggtataagcg
    16081 aaggctcaaa cattgctgtt atgcacggct tgggtctgct ttccaccgaa aacaagatgg
    16141 cgcgcatcaa cgatttagcc gatatggcgc aactcaaaga ctatgccgca gcagccatcc
    16201 gcgattgggc agtccaaaac cccaatgccg cacaaggcat agaagccgtc agcaatatct
    16261 ttacggcagt catccccgtc aaagggattg gagctgttcg gggaaaatac ggcttgggcg
    16321 gcatcacggc acatcctatc aagcggtcgc agatgggcgc gatcgcattg ccgaaaggga
    16381 aatccgccgt cagcgacaat tttgccgatg cggcatacgc caaatacccg tccccttacc
    16441 attcccgaaa tatccgttca aacttggagc agcgttacgg caaagaaaac atcacctcct
    16501 caaccgtgcc gccgtcaaac ggaaagaatg tgaaactggc aaacaaacgc cacccgaaga
    16561 ccaaagtgcc gtttgacggt aaagggtttc cgaattttga aaaagacgta aaatacgata
    16621 cgagaattaa taccgctgta ccacaagtga atcctataga tgaacccgtc tttaatccta
    16681 aaggttctgt cggatcggct cattcttggt ctataactgc cagaattcaa tacgcaaaat
    16741 taccaaggca aggtagaatc agatatatcc cacctaaaaa ttactctcct tcagcaccgc
    16801 taccaaaagg acctaataat ggatatagtg ccgggaattt aagcaaaaaa gtcctgactt
    16861 tagtttacat aaacggcaaa ctcaaattcg ggccaagcag tctgccgtct tcggcattga
    16921 aagcacctgt tcccattgtc ggcaaggctg aggctccgcc cggaaaagct gtgcctgtcg
    16981 gcgaagcccg cattccttca aatccaaaag cggtaaaagg aacaaccgtt accctgtcgc
    17041 aggaacaacg cctagccaac ggagagctga taccgaaagg ctctgtggta acgtggtcgg
    17101 acaatgcggt gaaggttgtg cggcctgatg ggaaaaagcg gagatttaat agtcgggatt
    17161 gggaacagaa ggcattgccg aaaccgcaac taccgaaaga ttacagagat gacatcaaca
    17221 taggtggaaa taatcctaga tttaatcagc aacggcatga aaaaatcgaa tcccacaacc
    17281 caaacagaat agaggagata cgccaacgta atgccgtttc ttctgcagaa aggcaggctg
    17341 atgcattaaa aaggatgcaa acgtctggcc gcacccgcct gcctgggcag ccacaaaaag
    17401 gaaaacccgg cgctgctaga gaaatgtcct cttcagacaa tccgaatcaa acagccgagg
    17461 cttttgcacg tcgatattta ggcaggaaac ctacactaat ggaacgagtc gaaggtgaaa
    17521 aattatacgg aggtaaatgt gtgggctgtt ggaaagcgca aatgccggac ggaacctttg
    17581 tggtgtatag gccagcaggt aatgcttcta tgaccaaggg aactactgcg tctgttgata
    17641 ttaataaccc aacattaaat aacttaaata ttaatttaaa aggcaagcca acagagttaa
    17701 aatttaaatt ccctgctaga taaggatgtt ttaatgaatt tagagacagc agaaaatatt
    17761 caatgcgaat ttgagtattt agcattagat ggttattttc caatgcattt tgcatcgcat
    17821 ggtcaaggaa acaaagattg gcaatttgca gttgaattca tatatagatt attaatatgt
    17881 caattagcaa ctcttgaacc tatcaacttt gaaacgaaaa atgatatttt agacttttgc
    17941 cataatttag caaaacaaag tccttttaat aatgataatg aggtgtggta tcagggggaa
    18001 attgtattaa caaaaaaagg gatagattta ataaaggaat atattccaga ggcgtttgaa
    18061 gagtggaatg gaaagaaatt tgaattaaac attcctttca ttaaaacatt aaaaaatata
    18121 tttgttaatt atgaggtagc atgggatgag aataatccac tattccccat tatatcttta
    18181 aacctcggca cctgataaac atggggttta tcaagcgaca gtggaaatta aaaagcctga
    18241 tggaagttgg gaaatgaaaa aaggaagggg cggaaaacct atgacaaatc attctatgtt
    18301 cccaaaagat tgggatgagg ctagaattag ggctgaagtt acttcggctt gggagaaaag
    18361 aaaggaatta gaaggtcaaa aatggagagg tacaagtaaa tctggtgttg aaatagaagg
    18421 atttacggaa cctaatcgaa cagcttatcc actttataat agtaaatgaa gtattgatta
    18481 aaaaggtgtt tttaaatgta taaaataaaa tgtttttatg atcagtttag ttctggtgaa
    18541 ttatttaact actgccagtt tttagataat tcttctaatc aaaagataaa tacacgagga
    18601 acggtgtatc aatatat
//
LOCUS       DAWKQJ010000026        18382 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000026 DAWKQJ010000000
VERSION     DAWKQJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.026"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(151..1083)
                     /gene="cysK"
                     /locus_tag="WJL20_000666"
     CDS             complement(151..1083)
                     /gene="cysK"
                     /locus_tag="WJL20_000666"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ7682844.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1277..1882)
                     /locus_tag="WJL20_000667"
     CDS             complement(1277..1882)
                     /locus_tag="WJL20_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682845.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="WJL20_000668"
     CDS             complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="WJL20_000668"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ7682846.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2867..3163
                     /locus_tag="WJL20_000669"
     CDS             2867..3163
                     /locus_tag="WJL20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682847.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3177..3845
                     /locus_tag="WJL20_000670"
     CDS             3177..3845
                     /locus_tag="WJL20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7682848.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3918..6041)
                     /gene="pnp"
                     /locus_tag="WJL20_000671"
     CDS             complement(3918..6041)
                     /gene="pnp"
                     /locus_tag="WJL20_000671"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ7682849.1"
                     /translation="MMFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVREIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6275..7138)
                     /locus_tag="WJL20_000672"
     CDS             complement(6275..7138)
                     /locus_tag="WJL20_000672"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ7682850.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPTDQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7337..8212
                     /gene="rfbD"
                     /locus_tag="WJL20_000673"
     CDS             7337..8212
                     /gene="rfbD"
                     /locus_tag="WJL20_000673"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ7682851.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLLSSGY"
     gene            complement(8927..9160)
                     /locus_tag="WJL20_000674"
     CDS             complement(8927..9160)
                     /locus_tag="WJL20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ7682852.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9326..9526)
                     /locus_tag="WJL20_000675"
     CDS             complement(9326..9526)
                     /locus_tag="WJL20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ7682853.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(9698..10573)
                     /gene="xerD"
                     /locus_tag="WJL20_000676"
     CDS             complement(9698..10573)
                     /gene="xerD"
                     /locus_tag="WJL20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ7682854.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10625..11065)
                     /locus_tag="WJL20_000677"
     CDS             complement(10625..11065)
                     /locus_tag="WJL20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ7682855.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11386..12324
                     /locus_tag="WJL20_000678"
     CDS             11386..12324
                     /locus_tag="WJL20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ7682856.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12453..12746
                     /gene="hfq"
                     /locus_tag="WJL20_000679"
     CDS             12453..12746
                     /gene="hfq"
                     /locus_tag="WJL20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ7682857.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            12831..13397
                     /locus_tag="WJL20_000680"
     CDS             12831..13397
                     /locus_tag="WJL20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7682858.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            13451..13825
                     /locus_tag="WJL20_000681"
     CDS             13451..13825
                     /locus_tag="WJL20_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ7682859.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            13835..14329
                     /gene="folK"
                     /locus_tag="WJL20_000682"
     CDS             13835..14329
                     /gene="folK"
                     /locus_tag="WJL20_000682"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ7682860.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPCAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(14456..14935)
                     /locus_tag="WJL20_000683"
     CDS             complement(14456..14935)
                     /locus_tag="WJL20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ7682861.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(15154..15891)
                     /gene="ubiE"
                     /locus_tag="WJL20_000684"
     CDS             complement(15154..15891)
                     /gene="ubiE"
                     /locus_tag="WJL20_000684"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ7682862.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(15934..16302)
                     /locus_tag="WJL20_000685"
     CDS             complement(15934..16302)
                     /locus_tag="WJL20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ7682863.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            16422..18041
                     /gene="mnmC"
                     /locus_tag="WJL20_000686"
     CDS             16422..18041
                     /gene="mnmC"
                     /locus_tag="WJL20_000686"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ7682864.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLREHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         4699 a         5437 c         3942 g         4304 t
ORIGIN      
        1 aaatatttcc ctcaagcaat gcggcggcac caatacctga ttaaaacaca accggtgttt
       61 tcggcatata tgcaaaaacg cattttccgg cacggcgtta caccgatacc gaaaaatgcg
      121 tctgaatgtt tcgacaatcc gattaaagca ttatgccaaa tctgcaaaaa gtggcgtaga
      181 gagatagcgt tcgccataag aaggcagcag cacgactatc agcttgcctt cgttttcagg
      241 ctgtttggca agctgcaacg cactccaaac cgccgcaccg gaagaaatac ccaccaaaat
      301 gccttctttt tccgctattg cgcgggcggt ttcaaaagcc gcttcgttcg acactttggt
      361 aatgctgtcg taaattttgg tattcaaaac ggtcggaata aagcctgcgc cgatgccttg
      421 aatcgggtgc gggccttttt cgccgccgct caatacgggt gaagcttcag gctcgacggc
      481 aaccacttta acttcgggtt tgtatttttt caacacttcg cccacgccgg taatcgtacc
      541 gcccgtgccg acgccggcaa cgaagacatc gactttgccg tccgtatccc gccaaatttc
      601 ctcggcggtt gttttgcggt ggacttcggg gtttgcctca ttgtcgaact ggcgcggcat
      661 aaaataagtg tcgggatgcg cgtccaccaa ggatttcgct ttggcaatcg cgcccgccat
      721 accttcggcg gcaggggtca gaatcagctc cgcaccaaac gcgcgcaaca gcattttgcg
      781 ctccttactc atgctttccg gcatggtaat cgccagcttg tagccgcgtg cggcacatac
      841 cattgccaaa ccgacacccg tattgccgct ggttgcttcg acaatgacgg tgtttttgtt
      901 gattttgccc gctttttcgg caccctcgat cattgcttcg gcaatgcggt ctttgacgct
      961 gctgcctgga ttgaaaaatt ccagtttcac ggcaacctcc gccttcaaac cttcggtcag
     1021 acggttcagt ttgaccaaag gcgtgttgcc gattagttcg gtgatgctgt ttgcaatttt
     1081 catcttctgc tcctgaataa taaggtttcg acagtaaggc gcaatcccca taggcatcct
     1141 gtccgattgc aggggatttg cgccgaaaca taaacaaatt tcggatatgg ctttatccta
     1201 aacctatgcc gcctaaaaaa ccaatatcca aaatttcttt ctttataact ttttgttccg
     1261 atacggccgc accgtattat cttccaattt ctgaaagcag cagcaccagt accgagacct
     1321 gcatcaacgc tgccgtcaac ccgccgagtc gcagctgcga gccgccgaac gttgacggcg
     1381 gctgccggtc tataccgcag cggtggacat aggcaaccgc catcagcaca aactgcaaca
     1441 cgaaccaata aaccggcttc agcgcataaa gtttgagcag tgccgcaaac acaaacactt
     1501 gaaccgttcc cgaaaagcaa taatgcacca acgcacatat tccgacaaaa gcaagcgtca
     1561 tcgatacatt actgacggca aacagcccga gcagaggatg ttcggggtct gcctgccatc
     1621 cgtttccatc cgttttccgg ttccaatgcc cgatgaaaaa aaatatgctg cccaaagtca
     1681 ggtaaaaatg ggggatgaac aagggcgcgg cgcgggtcat tccccatatg ccgggcatca
     1741 gctttgcccc caaaaccgat atgcccagcc acagcataat actcgcccaa aaccattgca
     1801 acattcctgc gcggaatatc agaaacagaa aaaccgccgc atacagtaag gcagacaaaa
     1861 aatccaaatg cagcaaaatc atgaatacgc caactcgcct tcaaacaccg tttccgcagg
     1921 gccggtcatc atcacatcgc cgccgcaggc ccattcgata tataaagttc cgcccggcaa
     1981 aatcacttct accgttttcc cttcatccaa caaccccaga cggatacccg ccaccacagc
     2041 cgcacacgcg cccgtaccgc acgcttgggt ttcgcccacg ccgcgctcga acacgcgcaa
     2101 acgaatcgcg gttcggctga caatctgcat aaagccgacg ttgacgcgtt cgggaaactg
     2161 cctgtgcggt tcgataagcg aaccggtttc gcgcaccggc gcgcattcta cgtcatcgac
     2221 cacaatcacc gcatgggggt tgcccatatt gacgcagctg acaggctgaa tgccggattc
     2281 gagatgcacc ccgtaaatac aggcatcatc cccctcgccc gattcgggga caaacggtat
     2341 ttcagacggc ataaacttcg gtttgcccat attgaccgta accataccgt tatcggacaa
     2401 tttcggaaaa ataacgccat ttgccgtttc aacacaaatt tctttcttat cggtcaaacc
     2461 cttgtctgca acaaaacggg caaaacaacg cgctccgttg ccgcattgcc cgacctcgct
     2521 gccgtcggca ttgaaaatac ggtaacggaa atccacgcct tcggtttccg aacgcccgac
     2581 caccaaaagc tggtcgaagc ccacgccccg gaaacggtcc gcccatgccg caatcggcgc
     2641 gtcctcgggg gtaaaatcct gactgaccgc atcaatcacc ataaaatcgt tgcccaaacc
     2701 gtgcattttg gtaaatttca aagtgttcat attgttccga aatcccgtat cgtctgttgc
     2761 ttccgaatcc gaaatccccc gcaccgaagg gaaatgcttc catctcccgc cgcctgagga
     2821 ttaaaatcag ccattatact gtttgttacc ccttacaaga aacaccatgt tccgcatatt
     2881 actgttgctt ttcccttgtg caatccttgc cgcctgcacc cgaaacatac cggcaaaccc
     2941 ggccgtttca aattgcgcgc atcttcaaaa ccgcaccatc ctgtattttg acgcacacgg
     3001 caaacccgcc gacggcgcag taaagggcgg attttacaaa attatcgaaa tacagggtgc
     3061 aaaccgcatc cttattcaag actttaacag cacgggcaat gccctcggcg gatcctatgt
     3121 gccggactgc cgcaaatcat cccttttccc gaaaaatatt tgacttcaaa ctgcctatga
     3181 accacgacat cactttcctc accctgttcc tgctcggctt cttcggcgga acgcactgca
     3241 tcggcatgtg cggcggatta agcagcgcgt ttgcgctcca actccccccg catatcaacc
     3301 gcttttggct gatcctgctg cttaacacag gacgggtaag cagctatacg gcaatcggcc
     3361 tgatactcgg attaatcgga caggtcggcg tttcactcga ccaaacccgc gtcctgcaga
     3421 atattttata cacggccgcc aacctcctgc tgctcttttt aggcttatac ttgagcggta
     3481 tttcttcctt ggcggcaaaa atcgagaaaa tcggcaaacc gatatggcga aacctgaacc
     3541 cgatactcaa ccggctgtta cccataaaat ccatacccgc ctgccttgcg gtcggaatat
     3601 tatggggttg gctgccgtgc ggactggttt acagcgcgtc gctttacgcg ctgggaagcg
     3661 gtagtgcggc aacgggcggg ttatatatgc ttgcctttgc actgggtacg ctgcccaatc
     3721 ttttggcaat cggcattttt tccctgcaac tgaaaaaaat catgcaaaac cgatatatcc
     3781 gcctgtgtac gggattatcc gtatcattat gggcattatg gaaacttgcc gtcctgtggc
     3841 tgtaagcccc attcattccc gatacccgta aaaaataccg tctgaaacct gttcagacgg
     3901 cactttcacc ctaagcgtta ctcggcggca ttttcctcac gggcaggcgc gtccagcagg
     3961 gctttgatgg acagacggac acggcctctg tcgtccactt ccaatgcttt cacgttcacc
     4021 acctgaccga cttgcaggta gtcgccgaca ttgcgtacgc gctcgtgggc gatttggctg
     4081 atgtgtacca aaccgtcttt gcccggcatc acgctgacaa tcgcgccgac attgttgtcg
     4141 aggattttca ccacagtgcc ttcgtacact ttgcccactt ccacttcggc agtaatctgc
     4201 tcgatgcgtt ttttcgccgc atcgccggct tcttgagtgg ttgcggcaat ggtaatcgta
     4261 ccgtcttcgg caatattgat ttccgtaccg gtttcagcgg taatcgcacg gatggtttca
     4321 ccgcccttac cgataacttc gcggattttg tcttggttga ttttcatcgt gaacaagcgt
     4381 ggcgcgtgtg cggacagctc ttgcgggccc gcaacggcgg ctttcatctg atccaagatg
     4441 tgcagacgcg cttctttggc ctgtgccaaa gcgatttgca taatttcttt ggtaatgcct
     4501 tggattttga tgtccatttg cagcgcggta acgccttcgg tcgtaccggc cactttaaag
     4561 tccatatcgc ccaagtggtc ttcgtcgccc aaaatgtcgg tcaggacggc aaatttgttg
     4621 ccttccagaa tcagacccat cgcgataccg gcaacgtgtg ctttcaaagg cacgccggca
     4681 gacagcaggc tcaggcagcc gccgcagacg gaagccatag aggaagagcc gttggattcg
     4741 gtaatttcgg agaccacgcg catggtgtag ctgaaatctt caggtttcgg caatacggcc
     4801 aacaatgcac gtttagccaa acggccgtga ccgatttcac ggcgtttcgg tgcgcccatg
     4861 cggcccactt cgccggtaga gtacggcgga aagttgtagt gcagcataaa gcggtcggtg
     4921 tattcgccgg acagcgcgtc gatgatttgc tcgtcgcgcg aagtacccaa agttgcaacg
     4981 gccaaagctt gggtttcgcc acgggtaaac aatgcagaac cgtgcgtgcg cggcaatacg
     5041 ccggtttgaa tgttcagcgg acgaacagtg cgggtgtcgc gaccatcaat acgcggttgg
     5101 ccgtctaaaa tttggctgcg gacaacatcg gcttccaagt gtttgaaaat gcctttgatt
     5161 tcgttggctg ccaaagtgtc ggtttcctcg gtaatcaagg cttcttttac agcactccaa
     5221 gcttcgtcca atttggcaga acgcgcttgt ttttgacgga ttttgaatgc ttctttaatg
     5281 gtttcaccgg caatttcgcg tacttttgca accaattctt cattggtttc aggtgctttc
     5341 caatcccaaa cttccggatt gacttcatcg gcaaactcat tgatggcgtt aatggccact
     5401 tgcatttgat cgtgaccgta aaccaccgcg cccaacatca cgtcttcggg caggattttg
     5461 gcttcggatt ccaccatcaa cacggctttg gaagtaccgg cgaccaccaa gtccaattgc
     5521 gatttcgcca attcggcttt agtcgggttc aaaacgtaca cgccgtttac ataaccgacg
     5581 cgtgccgcgc cgatcgggcc ggcaaacggt acgccgctca acaccagcgc ggcagacgcg
     5641 cccaacattg caggaatatc agaatcgatt tcaggatcga cggacacgac catcgctacg
     5701 atttggatgt cgtggtagaa accttcaggg aacagcggac gaatcggacg gtcgatcaga
     5761 cggctggtca ggatttcttt ttcgctttgt ttgccttcgc gtttgaagaa accgccggga
     5821 attttgcctg cggcgtaagt gcgctcgagg taatcgacag tcagagggaa gaagtcttgg
     5881 ccttctttca cttctttgtt ggtggtaacg gcaaccaaaa ccacggtgtc gcccatagag
     5941 actttaacgg cagcggcggc ttggcgggca atttcgccgg tttccaaagt aacggtgtgg
     6001 ttgccgtatt ggaaggtctt aacgtgtttg tcgaacatca ttgttccttt caaaataccg
     6061 cactgctaaa acactaataa tgcacactaa aatccgaatg tgcatagtta gggtttcaga
     6121 ccgtgcggca ggttgtaaac aagctttcag acgacatttc ggacgctgaa agcaattcca
     6181 gattataaag gcaaccatcc ttaaaatcca gtattgatac aataaaaagg ccgtctgaac
     6241 catatttctt tcagacggcc ttcaaactta aaaatcaatc ctgagtcagc aaagtcaatg
     6301 cttcccgata cttctcgaca gttttctgaa tcacatcggc aggcactttc ggcgcagggg
     6361 cttttttgtt ccaaccgctt tgttccagcc agtcgcggac gaattgtttg tcaaaagacg
     6421 gcggattggt gccgactttg tattggtcgg taggccaaaa acggctcgaa tcgggagtca
     6481 atacctcatc catcagcgtc agcgtaccgt ttgtatccaa accgaattca aatttggtat
     6541 cgcaaataat aataccgcgc gatttggcat attccgccgc ttcggtgtaa agccgaaccg
     6601 ccttggcgcg cacttcttcc gccaattctt tgccgataat gcgtccgcat tcttcaaagc
     6661 tgatgttttc atcgtgatcg ccgactgcgg ctttggttga gggcgtaaaa atcacttcag
     6721 gcagttgttg cgcttcctgc ataccttcag gcagttgaat accgcaaacc gagccggttt
     6781 tttgataatc tttccaaccg ctgcctgcca gataaccacg cacaatcgcc tctactttca
     6841 ccggagtgag ctttttagcc acgacggcgc gtttctctaa agctttggct tcgttttcag
     6901 gcaaaacatc gtaaaccgtt tgaccggtaa agtggttggg cataatatgc gccagttttt
     6961 taaaccaaaa attggaaatc tgcgtcagaa tctccccttt gctcggaatc gggtcgtcca
     7021 aaatcacatc aaacgcggac aggcggtcgg aagcgaccat cagcatacgt ttatcgtcga
     7081 tttcatataa atcgcgcact tttccagaat agatctttac caaaccaatc tcactcattt
     7141 cgccccccct gaaaatatct tgaaaatacc gacccgacac ccgacaggtt tgaatcacaa
     7201 accgatattc taaccgaagt cggcgcaaaa caatacccat ggcacaaaaa gccaacccgt
     7261 caaccgtcgg caaagttttg gcactataat accgacagca agtcctacaa tacactttca
     7321 ccaaaggaaa tacctcatga gaatcctatt gacaggctcg aaaagccaac tggcacgctg
     7381 cctgcgcgac cgtcttccgg aagactggga aaccattgcg acggattccg catccctaga
     7441 cattaccgat gccgatgccg tctgcaacat ggtcaaaagt ttccaacccg acgccattgt
     7501 caacacggct gcctatactg ccgtcgacaa agcggaaggc gatgcggcag cggcatttgc
     7561 cgtcaatgct tccgccgttt acaaccttgc cttggcagca catcgcgccc atgcccgatt
     7621 catccacatc tcaaccgact atatctttga cggtaaaggg aaaaggccct atcaggaaag
     7681 cgactttacc aatccttcca atgtatacgg acaatccaaa accgccggcg aactgctcgc
     7741 actgtctgcc aatcccgaca gccttatcct gcggacttct tggctgttta gcgaatacgg
     7801 ggacaacttt gtccgcacca tgctgaacct tgcgcgggaa cgttccccgc tgtccgccgt
     7861 ccacaaccaa atcggctgcc cgacctatgc cggcgacctg gctgccgcca tcatccgcct
     7921 tttgcagcaa acgtcgcccg tgcgcggcat ttaccactac gccggcggca aatccgtatc
     7981 ctggtacgaa tttgcccaac atattttcca agcggcatcg caacagcaga catccttccc
     8041 cgttcccgaa ttgactgccg tttcagacaa ggaatatccg accgccgccc ccaggcccgc
     8101 atacagcatt ttggactgcc gcaaaatcga aaacgacttc ggcatcaaac cgtcagactg
     8161 gcagaaagcc cttgcacagg tcgtttccaa gctgctctct agtgggtact agatataatg
     8221 cagcatccga taaagtaaag ccccgaagtg gcagcttcgg ggcttatcgg atagaaagga
     8281 ggcttgaaga aatggcgaaa gttattttat tcctgtttct tttgttagtt tgctcgccag
     8341 cctactaatg aaaaactgaa ataacaaggg gtggcaaatt accgcgcccc tttcgccaac
     8401 ctttcttcga gccattttac tacaatggtt ttaaaaatca aggattgcct gatgccgccc
     8461 gcccctctgt ttcctccgtc aagcaccgcc ttggcggttt tcttatatag tggattaaca
     8521 aaaaccagta ctgcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     8581 ccttgtcctg atttttgtta atccactata aaatctaccg attacacaaa cacatatcat
     8641 ctttacacaa tcatgcttcc atcaacagta aaacatgata tgattgccaa caataacatc
     8701 tcacaataaa ttttctaatt tttattgaaa aaatcaataa ataagaattc tcccaaccga
     8761 caaataaaat aaagaaaggg ttaatatgca acatcgaaga agactagcaa tttaccaagc
     8821 atccaaacgt gcttccttta ccggcaggtc atcagccccg caaaaacgta aagaacgtta
     8881 atttgaaaaa aatgccgtct gaagtcctgc ttcagacggc atttttttac cgttcgagaa
     8941 actgtttcaa cctgtcctca tccaaaaacc agtggcagat ttcctcatct cccgccaaca
     9001 aaacgggaac cagctcattg tatttttctt ccaaaacagg attttcatcc acatcgacca
     9061 cttccagccc gaacccgtat tcattttgaa aaggtttgag tgcatcgcgc attttgtggc
     9121 acaagctgca atattcacga aacatcaagg tcaatttcat ccgcgtttcc ttatctgtca
     9181 atttgcacac gccaaagcct tagacgcagc agaatcatgg tctatttggg aaaaaacaat
     9241 gttttcaagg aagatgatac tcaagtcctg ccaaaaacag caaaaatgcc gtctgaacag
     9301 ttcagacggc atttccaaaa ccgttttacg cttgaacatt gatacccgcc gtaaccgtcg
     9361 gttgcgcatt gtgcgccgtc agaaacgaag cggcaagctc cccacagcag ccgcagcagc
     9421 tcgctacgcc caattgcgac tgcaaatcgc ccattgtggt cgcgccggcg gcgatggttt
     9481 ccttgatttg atggtcggta acggcattgc agatgcagac aaacattttg tgctccgtgt
     9541 gttctcaaac tatcgtaccg atagcggctt ttattatcgt atgcgaatat aaataaaaac
     9601 ggttcgcatt gcaaggtcgg tatacacggt ttgtcttggt aattttttat caagtttgca
     9661 ttttcgattg atttcttatt aaaccaaagt agaagcctca gtttcgggaa tggtgttcct
     9721 tcactacacc ctgcaaccat acgttggcaa catgggtata aatctgcgtc gtattcaaat
     9781 cggcatgtcc caacatatcc tgaaccacgc gcaaatccaa gccgtgccgc accagatgcg
     9841 tggcaaaggc gtggcgcagg ctgtgcgggc tgatgtgccc gatgcctgcc tgacttgcat
     9901 attctttgac aatcatccat gccaactgac gggaaatgcc cgtctttttc tgactgacaa
     9961 acaatgcgtc gcaattcctg cctttcagca gaagtgggcg tgcctccgta taatagcgtt
    10021 ccacccaata cgccgactcc tgccccatcg ggaccatcct ctgcttatca ccctttccca
    10081 gcgcggtaat acagcccctg tccaaatcca cattgccgaa gttcagcccg accgcctcgc
    10141 tgacgcgcaa gccggtcgcg tacatcaatt cgagcaaagc cttgtcccgc aaaccgtgcg
    10201 gcgtgtcggt atccggggcg gcaagcagtc gggaaatctg ctgctcggtg atcagggtcg
    10261 gaatattctt gtcgattttg ggcggtttca gcaaacgggt gggattgtcc gtccttatgc
    10321 cttcacgctc catccatata tacaggcgtt tgcatgccga taatgcgcgc gcctgcgaac
    10381 tccgttgctc tccgtcaaca taaaccgccg ccgccaaatc cgcttcgtcc gcatccttca
    10441 gcattctgcc cgattgggac aggcggcggg cgattttttc caaatcgcgc cggtaaccgt
    10501 ttaaagtatt ctgactgagc cgcctgtcca accacagcgt ttcaagcagc ctgtcgatca
    10561 aaccttcttc cataccgttc caaacaaatg ccgtctgaat cttcttcaga cggcatggtt
    10621 cacattatcg ggaaagcgtt tccaatactt cctgcgcgtg acccgccact ttgactttcc
    10681 gccattcatg ggcgatttct ccatccttat tcaagacgaa cgtactgcgc tcgataccta
    10741 acgactcttt cccgtacagt ttcttcaatt tgatgacatc aaacaggcgg cacactgttt
    10801 catccttgtc gctcaacagc tcgaaccgga aaccctgctt ggcgcaaaaa ttctgatgcg
    10861 cctttacgcc gtcgcgggaa ataccgacca cggtataacc caatgcctca aactgttcca
    10921 aacgcgcatt gaaatccaag ccttccgtcg tacagcccgg cgtactgtct ttcggataaa
    10981 aatacacgac caaaggcaga tgttctgccg aatgaaaatc cgcaccgctg ctcgaaggca
    11041 gggtaaattc atatttcaca tccatagtcc tactcccgat attcccatta ttcaaaacga
    11101 cacgcagacg caccgccgca attgccaaac caaccccgat tctaccgccc caaaggacag
    11161 ggattcaacc gccggaaaca tccaaaccga cacacgacgg catgaaaaat atccatgtca
    11221 aaccacaaaa tatgttccga tttaaaaaca gaatgttata aaacccaatc cccaaaacac
    11281 aacaagaccg cccgcctacg ggcagtctcc tgtcagacga catactttac agatggctgt
    11341 tttttcaaca aaataacgcc aatactcaaa aatatggaat caaaaatgtc catccatact
    11401 ctgaaacgcc tgccctcatc gctgctgctc ggtctctgcc tttccctgcc gtcagcccac
    11461 ctttttgccg acaacgacat tttagggcaa tttttagaac agaacatgct tacctcctcc
    11521 gatccgatag aaatattcgc cgaaagcacg atacacccca ccaacaccca agccattaca
    11581 ggcggtctga ttctctcctc acagtctgcc ctggtcgtca acaacaaaac cggacagata
    11641 ctgtatcaga aaaacgccga caggattatg cccatcgcct ccatttccaa actgatgagc
    11701 gcgatggtcg ttttggatgc aaacttggac atgaacgaaa ccgttaccat tacgcccgac
    11761 gaaatcgacc gcatcaaagg gaccggcagc cgtcttgcca taggtacggc acttacacgc
    11821 aaaaaactgc tgcacctgag cctgatgagc agcgaaaacc gcgccaccca tgcattgggc
    11881 agaacctacc ccggcggcat gggcgcattt gtcgccgcca tgaaccgcaa agcccaaagc
    11941 ctcggtatgt acggcagccg cttttacgaa ccgaccggac tcaacttcca aaacgtttct
    12001 accgccaaag acctgagcct tatggtcaac gccgccgccc aatatccgca aatccgcacc
    12061 aactcgactt ccaactacgc ctcggtacag accaaaaacg ggcagcagaa ctacaaaaac
    12121 tccaatgccc tggtcagaga aggcatgtgg aacatcgaat tgcagaaaac cggctacata
    12181 cgcgaagcag gcaggtctat ggttgtcaaa gccaacattc aaaaccaacc cgttaccatc
    12241 gtattgctga actcgcccac atccgccaca cgcgtcaacg acgcccgcaa aatcgaatcg
    12301 tggatgctgc agcaacgctc ctgacataca aatgcccggc ggaaaaccgc ccaaacacaa
    12361 aaaaaattgg taatttttcc tattcaaggc tacaattcga cacgcacagg gcgtctgccc
    12421 gatttcacaa tcataataac ggagttaaaa atatgacagc taaaggacaa atgttgcaag
    12481 atcccttcct gaacgccctg cgtaaagagc acgttccggt ttcgatttac ttagttaacg
    12541 gtatcaaatt acaaggtcag gttgagtctt tcgatcaata cgttgttctc ctgagaaaca
    12601 cttccgtcac ccaaatggtt tacaaacacg ccatttccac catcgtaccg gcacgctccg
    12661 tcaacctaca acatgaaaac agaccccaag ccgcaccgac ttcgaccctc gtccaagtgg
    12721 aaaccgtcca gcagcctgcc gaataatccg cacgaagcat gacgtgtcat atctttcaat
    12781 accttaccgg acaacggtaa ggtattttta ttttcagaca gcatttaaaa atgttattgc
    12841 aaaacatcct tccattcgcc cattgccttt tgcggaaggc acttcccgaa ggtggcaatg
    12901 ctttggacgg caccgccggc aacggacacg acaccctttt cctcgcacaa accgcaggca
    12961 tccgggggaa agtgtgggca ttcgacatcc agccgcaagc cctgagccgc actcgatgcc
    13021 gtctgaaaga agcgagatac gagaatgtgc aactcatctt agacggacac gaaaacctga
    13081 aacaatatgt ttcagaacca ctaaatgccg ccattttcaa ctttggctgg ctgcccggcg
    13141 gagataaaag ccttaccacc catacggaaa ccagcattcc cgccctttgc gcaaccctct
    13201 ccctgctgaa agaaggcggt atgcttattg ccgtcctcta tccgggacac gaaaacggca
    13261 aacaggaggc agaagcaatc gaacaatggg caaaaaacct gcctcaagaa cagtttgccg
    13321 ttttgcgtta cggctttacc aaccggaaaa acaacccgcc ctatctgttg gcatttgaaa
    13381 agttacgtca aaaataaatg tttgcggtaa aataagccga ttctaaccct tattttgata
    13441 cgagccgacc atgcagtatc tgattgtcaa atacagccat caaatcttcg ttaccatcac
    13501 cattttggta ttcaacatcc gtgttttcct actttggaaa aatccagaaa agcccttggt
    13561 cggcttttgg aaggcactgc cccaccttaa cgacaccatg ctgctgttta cgggattgtg
    13621 gctgatgaaa attacccatt tctccccgtt caacgcgcct tggctcggta caaaaatcct
    13681 gcttctgctc gcctatatcg cattgggtat gatgatgatg cgcgcccgtc cgcgttcgac
    13741 caagttctac accgtttacc tgctcgccat gtgttgcgtc gcctgcatcg tttaccttgc
    13801 caaaaccaaa gtcctgcctt tctgaaacac cgttatgaac aacagacatt ttgccgtcat
    13861 cgccctgggc agtaatcttg aaaaccctgc ccaacaggta cgcgccgcat tggacacgct
    13921 gtcgtcccat cctgacatcc ggcttgaaca ggcttcctca ctgtatatga ccgcccccgt
    13981 cggttacgac aatcagcccg atttcgtcaa tgccgtctgc accgtttcca ccactctgga
    14041 cggcattgcc ctgcttgccg aactcaaccg tatcgaagcc gatttcggac gcgaacgcag
    14101 cttccgcaac gcgccgcgca cattggattt ggacattatc gactttgacg gcatctccag
    14161 cgacgacccc cgactcaccc tgccgcatcc gtgcgcgcac gaacgcagtt tcgtcatacg
    14221 ccctttggca gaaatcctcc ctgattttgt tttaggcaaa cacggaaagg ttgccgaatt
    14281 gtcaaaacgg ttgggcaatc aaggcatccg tcttttaccg gataagtaat tccgcacgcg
    14341 gatgccgtct gaatgccggt ttcagacggc atttttcctt tgccgccaac acgcgtgcaa
    14401 aaaaatcgcc ccttggaaaa ggggcgcaaa aggaacacaa accactacca aaactttaaa
    14461 tctgcaacat tgcctgccgc atactgtatc cgacaggata taaagccctc actaaatcgt
    14521 ttcgagaaat ccaaacttct tcatcgccga cagaaaatct gcctttctcc ggcaccagct
    14581 ccaacaaaaa cggttgaacc gccgtatgca gcctgtcctt acaccgcccc agctttctgg
    14641 acaaggcgga cagggcacgc ttatagacac tatccttgcg gtcaagttcc cgcgcactgc
    14701 cgacccagct caaacgaagg ttgcggcttt catcctcaat cagcaaaatg cctgttttga
    14761 cttcccccac ctctggctcg catgaaagcg aaatataata tttgccgtcg atgttgacat
    14821 acgccgcctc atgcacctga cagttgcagc cggaagtggc aagcagcgaa taaacagaat
    14881 cccgatgcag tttcaaaata tcaagggaaa tctgtttgaa aatactttcc ggcatttttc
    14941 cctcctttcg tctgctgcgt tgtttactgg attgactgca ttatacagac atctcccaat
    15001 cttgcaaatg ataattgttc ctatttttta aaaaatttat aaattattga tagatatgtg
    15061 aattaatttt aagatttaaa atctctttct caaaaaacac aaaaatgccg tctgaaacgt
    15121 gttcagacgg cattggctgc acagccagtc cgtttaaaat ttcacgccct tatgcagcgc
    15181 aacgatcccc gcactcatat tatgatagtc cacactgtca aatccggcat ccagcatcat
    15241 ttgctttaaa gtttcctgat cggggtgcat acggatggat tcggcgagat actggtagct
    15301 gtccgcatct ttggcaatca gtttgcccat gaccggcagc agcttgaacg aatagaaatc
    15361 atacgcgcct tcgagcggct tgtagatttt ggaaaactcc aacaccagca acgtgccgcc
    15421 aggtttcaaa acacgataca tctctttcag cgcggcatct ttatgcgtca tattccgcaa
    15481 accgaacgcc acggaaacca agttgaaata attgtcaggg aaaggcagtt tttccgcatc
    15541 ggcaagcgac acgggcaaaa tcatgccttc gttcaaaaga cggtcgcgcc cgacggtcag
    15601 catagaggaa ttaatatcgg tcagccaaac ctcgccttcc ttgccgaccc gtttcgccca
    15661 accgcgcgac aaatcgcccg taccgcccgc aatgtccaac actttatcgc cttttttcag
    15721 gtgcgccgtg ttgatggtga aatgcttcca caccctgtgc agccctgccg acatcacatc
    15781 gttcataatg tcgtagtttt tggcgacgga gtggaacact tccgccactt ttccggcttt
    15841 ctcatcttcg ttgaccgtgc tgaacccgaa atgcgttttc tgtccgccca tattctaacc
    15901 acctccctga tgccgcccta cggcatccca aacttaacaa tctgattgct tttcaaaacg
    15961 ggaagcaccc gccgccgcca atttgtccaa atattcctgc cacatcgcat cgtatccgta
    16021 tgccagtcta tgcagatacg cccaatcgta aagaccgctg tcgtgcccgt ccgaaaaact
    16081 gattttcagc gcgtactgtc cgacaggctg caaatccgtg atttggacat ccgccttgcc
    16141 ggtctgcaaa acatcctgtc ccacgccgtg tccgcgcact tccgcactcg gcgaatagac
    16201 gcgtaaaaat tcggcaggca gatttttagg ttcgtcccca taagtcaaaa ccaacacggt
    16261 acggtttttc aacaaacgga tttcaacagg aatattattc aaaaaacacc tcggttcggc
    16321 atacaatatc cggcaggcgt acgcgtgcct ttaaacggcg cgtattttaa cagctttatc
    16381 ccgataccga tattcaaacc cacactttaa acggcattga tatggacaat ctcgtatggg
    16441 acggcgttcc cgacatccgc acactcgacc aaaccatccg caaacacgca cacccgctca
    16501 acctgattgt ctgcctcccc gataatcaga ttcccgattt tcaaaccgca caagatgctt
    16561 cggacgcgga atgccgtctg aagcaccgtt tggatcaggc aacgcagtgc ctccagttcg
    16621 acagcatcaa cctcatcgaa cacatcctgc ccgatgtccg cttctggctg gttccccctt
    16681 cacgcacccg ccgcctgcgc gaacacttcc accacatttc ctggcagacc gaagccatcc
    16741 cgcaaaccga aagtaagccc gacaaaccct ggtttgcact tccacaaaca tccgaacggc
    16801 aaaaaccgga acacatcctc gttatcggcg cgggcatatc cggcgcggca accgcccacg
    16861 ccttagcatc gcacggcatt tccgttaccg tattggaagc ccgaaaagcc gcccaagccg
    16921 ccagcggcaa ccgccaaggg ctgctctacg ccaaaatctc gccgcacgac accgaacaga
    16981 ccgaactttt gcttgccggc tacggctaca ccaaacgcct gctcggacac atcctgcccg
    17041 aatccgaaac ctggggcggc aacggcatca tccacctcaa ttacagccgc accgaacaac
    17101 aacgcaatca cgaattgggt ttgcaaaaac accataacca cctctaccgc agcatcacgc
    17161 aggcagaagc cgaaaaaatc gccggtatcc cactgtccgt cccatacgac cacccttcat
    17221 gcggactcta ctggcaacac ggcgtatggc tcaatccacc cgcattcgtc cgcaccctcc
    17281 tcaaccatcc gctcattgga ctacacgaag acacaccgtt aaccgacatt tcccacgacg
    17341 gggaaaagtg gattgcaagc acgccaaacg gcatattttc cgccacacac atcatctact
    17401 gcaccggcgc gcacagcccc cgcctgcccg aaaccaacct cgccgccctg cctctcaggc
    17461 aaatacgcgg acaaaccggc ctcacaccgt ccaccccgtt ttccgaacaa ctgcgttgcg
    17521 ccgtttcagg cgaaagctac atcagcccgt cgtggcacgg actgcactgc tacggcgcga
    17581 gttttattcc caacagcagc cataccggat ggaacgaagc cgaagaagcc tcaaaccgcc
    17641 aagcattggc acaccttaac cccgcccttt ccgaatcatt gtttgccgcc aacccaaacc
    17701 cccaaaaaca ccaagggcac gccgccatac gctgcgacag ccccgaccac cttcccctag
    17761 tcggcgcact cggcgacatt gccgccatgc ggcagaccta caccaaactc gcgctggaca
    17821 aaaactaccg catcgacacc ccatgcccat acctgcctaa tgcctacgtc aacaccgcgc
    17881 acggcacccg cggactcgcc accgccccca tctgcgccgc cgccattgca gcccaaatcc
    17941 taggcttgcc ccatcccttt tcacaacgcc tgcgccacgc cctacacccc aaccgcacca
    18001 tcatccgcgc catcgtcaga aggaaggatc taacccctta agcctttgcc gcatgccgtc
    18061 ccgaccacag aaaatcatat tcaaataaac ataccaatcc tgacggtaaa atatcgaaaa
    18121 cacccataac gttttcttcc caaaataagg agtaaggaat gcctaacaaa ttgcctttgc
    18181 tgtccaagat gaatattccg taacattcga agacttcctt aaccctgcca gccgtttcgg
    18241 tttctgtacc ccctgttaga taaggaagga atgaaaaagc cctgttgatt gtatgaagta
    18301 aagggcgtta atttttgtta atcttccctt ctcaggaact caatatataa ggatgttaat
    18361 ttttatttcc cttctcgagg ac
//
LOCUS       DAWKQJ010000027        17556 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000027 DAWKQJ010000000
VERSION     DAWKQJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.027"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(165..1121)
                     /gene="gshB"
                     /locus_tag="WJL20_000687"
     CDS             complement(165..1121)
                     /gene="gshB"
                     /locus_tag="WJL20_000687"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ7682865.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVSYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1214..2902)
                     /locus_tag="WJL20_000688"
     CDS             complement(1214..2902)
                     /locus_tag="WJL20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ7682866.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(3014..3787)
                     /locus_tag="WJL20_000689"
     CDS             complement(3014..3787)
                     /locus_tag="WJL20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ7682867.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3830..4732)
                     /locus_tag="WJL20_000690"
     CDS             complement(3830..4732)
                     /locus_tag="WJL20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ7682868.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            complement(4881..5597)
                     /locus_tag="WJL20_000691"
     CDS             complement(4881..5597)
                     /locus_tag="WJL20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ7682869.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6156..6578)
                     /locus_tag="WJL20_000692"
     CDS             complement(6156..6578)
                     /locus_tag="WJL20_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ7682870.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6696..7367)
                     /locus_tag="WJL20_000693"
     CDS             complement(6696..7367)
                     /locus_tag="WJL20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ7682871.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(7383..8009)
                     /locus_tag="WJL20_000694"
     CDS             complement(7383..8009)
                     /locus_tag="WJL20_000694"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ7682872.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8116..8934)
                     /locus_tag="WJL20_000695"
     CDS             complement(8116..8934)
                     /locus_tag="WJL20_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7682873.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9193..9633)
                     /locus_tag="WJL20_000696"
     CDS             complement(9193..9633)
                     /locus_tag="WJL20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ7682874.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(9696..11102)
                     /locus_tag="WJL20_000697"
     CDS             complement(9696..11102)
                     /locus_tag="WJL20_000697"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ7682875.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            11272..12387
                     /gene="lptF"
                     /locus_tag="WJL20_000698"
     CDS             11272..12387
                     /gene="lptF"
                     /locus_tag="WJL20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ7682876.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            12384..13454
                     /gene="lptG"
                     /locus_tag="WJL20_000699"
     CDS             12384..13454
                     /gene="lptG"
                     /locus_tag="WJL20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ7682877.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13764..16349
                     /gene="acnB"
                     /locus_tag="WJL20_000700"
     CDS             13764..16349
                     /gene="acnB"
                     /locus_tag="WJL20_000700"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ7682878.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16491..17486
                     /locus_tag="WJL20_000701"
     CDS             16491..17486
                     /locus_tag="WJL20_000701"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ7682879.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         3981 a         4871 c         4625 g         4079 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact atatataccg tctgaaaacg gggcggcggg
       61 gtgtccgtac ggcattaacc gcatccctgc cggctgaggg aaaaccctgc ctgcccgaac
      121 aaaccaaacg gttgtgaagc aaaagccttt caaacggcat cggtttaacg taccgaccac
      181 gcggcaacgg catcggcaaa cattgccgcc acgtcgaaac ctttttgttt cataatttct
      241 tggaatccgg tcgggctggt tacgttgact tcggtcaggt tgctgccgat aacgtccaaa
      301 ccggccagca ggatgccgcg ccgtttgagt tcgggggcga gcgtttcggc aatttcgcgg
      361 tcgcgtccgt ccaattcctg cgccacgccg cgtccgcctg ccgcaaggtt gccgcgtgtt
      421 tcgccgtttt gcgggatacg cgccaaagca taggagacga cttcgccgcc gataatcagg
      481 atgcgtttgt caccgtgtac gatttcggga atgtagcgtt gcgccataat ggtgcgggaa
      541 tcaagccgca tcagggtttc gaggatgctg ccgatgttgg ggtctttttc ggtcaggcgg
      601 aagatgccca taccgcccat gccgtcgagc ggtttaatga tgatgtcgcc gtgttctttc
      661 aaaaatgcgc ggacatcggc ggaacgggtc gttaccagcg tgggcgcggt aaagcggctg
      721 aagttcaaaa tcgccagttt ttcattgaaa tcacgcatcg cctgtccgct gttaaagacc
      781 ttcgcgccct gctgctccgc cagcgtcaga agctgtgtgg cgtagaggta ttgcatatcg
      841 aacggcggat cggtacgcat aatcacggca tcaaatgctt tcaatgccgt ctgaactttg
      901 tcggcggcgg caaaccagtt tttgtcgtgg tcgtcttgtg cgccgagaaa ctcaaaagca
      961 gacgcatcag caaccaccag accgttttta accgacaatt ccccgctcaa ggtatgaaac
     1021 agccgccaac cgcgttttgc catctcgcgc atcatggcgt aggtggtgtc tttgtaggtt
     1081 ttgaaacttg ccatcgggtc ggcgataaag aggactttca tcatatttcc tttccggtgt
     1141 gccgaatgtg ccgcatttcg cgggcaaagg ggaaatgccg tccgaacaat attcagacgg
     1201 caaggatggg gttttactta ggctgccaag aatctttcag cgtgaccgtg cggttaaaca
     1261 ccggcgtgtc tttgccgtgg tctttacggt cggttacgaa gtagccgata cgctcgaact
     1321 gccaacggct ttctgccggc aaatctttgg cggcaggttc ggcgtaggcg gtgatttcct
     1381 tgacggattc cggattgagg aaatcggtga acggcaggta ttcgccgtct tcgccgcgca
     1441 cggcatcggg acgctcgacg gtaaagaggc ggtcgtacag acggactttg atttcggcgg
     1501 cgtgttcggc ggaaacccaa tgaatcacgc ctttaacttt acggccttct ggatttttgc
     1561 ccaaggtgtc gtggtcgatg ctgcatttga gttcaaccac attgcctgct tcgtctttga
     1621 cgacttcatc gcacttgatg acatagccgt ggcgcaagcg tacttcgccg ccgggaatca
     1681 ggcgtttgaa gcctttgggc ggattttcgg caaagtcgtc ggcttcgatg taaatggttt
     1741 gtgaaacagg tacttcgcga tcgcccattt cttcgtggtt cggatggaac gcggcacggc
     1801 ggctttgggt tctgccggtt tcaaagttgg tcagggttac tttgagcggg ttcaacaccg
     1861 ccatcaggcg tggggcggaa ttttccaact cttcgcgaat cgcgccttcc aacacgctca
     1921 tatcgacgat gttttcagat ttggaaatac cggcgcgttt ggcaaacagg cgcagccctt
     1981 cgggcgtgta gccgcgtcgg cgcataccgg aaatggtcgg catacgcgga tcgtcccagc
     2041 cggaaacgtg tttttccaca accaactgat tcaatttccg tttggaggta atggtgtaca
     2101 aaagctccaa gcgtgaaaac tcgtattggc gcggacgtgt ggcgtgcggc gcggggatgt
     2161 tgtccaacac ccagtcatac agcggacgat gggcttcaaa ttcgagcgta cacaaggaat
     2221 gcgtgatgcc ttcgatggca tcggaaatac aatgcgtgta gtcgtacatc ggatagatgc
     2281 accatttgtc gccggtgttg tggtgatggg cgcggcggat gcggtagatg accggatcgc
     2341 gcatattgat gttgccggat gccatgtcga ttttcaggcg cagggttttg ctgccgtcgg
     2401 ggaactcacc gtttttcatg cgcgtgaaca ggtcgaggtt ttcttcgata ctgcggtcgc
     2461 gataagggct gtttttgcct gcttcggtca gcgtaccgcg gtattcgcgc atttcttctg
     2521 gcgtcaaatc atcgacatac gctttgccgt ctttaaccaa accgacggcg tagtcataaa
     2581 gctggtcgaa atagttggaa gcgaaacgcg gctcgcccgc ccaatggaaa ccgagccact
     2641 caacgtcttc tttgatggcg ttgacgtatt cgtcgttttc tttttcgggg ttggtatcgt
     2701 caaaacgcag gttgcacaag ccgtcgtaaa tatacgccaa accgaagttc aggcagatgg
     2761 atttggcgtg tccgatgtgc aggtagccgt tgggctccgg cggaaaacgg gtttggatgg
     2821 tttcgtgttt gccgcttttg aggtcttctt cgatgaaggt gcggataaaa tggttgtccg
     2881 caaattggtc tttattgagc atagttttct ttgaacagat ggcttcagac ggcattgggg
     2941 cggttcctta tgccgtctga agcggtttga tgatgtgttt attgtacccg acttgcgcgc
     3001 tttgacatag cgttcagacg gcatcggcaa tcaggcattc cacccccgcc tctttcagca
     3061 tcttctgcat cgcggtatcg ggcagccggt cggtaaatac tttgtcaaac gccgtaatgt
     3121 cgccgagcct gaccagcgcg ttgctgcgga atttactgtg gtccacgccg aggaagcgga
     3181 cgcgcgcatt ggcaatcatc gcctgcatca cgctgacttc tttgtagtcg tcgtccaaaa
     3241 gcgaaccgtc gctttccacg ccgtgcgtac tcatcacggc ataatcgact ttgaactggt
     3301 tgataaaatc gacggttgcc acgccggtaa taccgccgtc caaagggcgg acgactccgg
     3361 aagtgataat gaccgtgtaa tccgtccgcg ccgaaacgat ggaggcgacg tggatattgt
     3421 tggtaataat ccgcaggctg ccgcgccgcc tgaccagctc cgatgccacg gcttccatcg
     3481 tcgtgccgat actgacaaac agcgacgaac cgtcggggat gtgttccgca atcagccggg
     3541 caatggcgtt tttttcgttt tgacattgaa tttggcggct ggcaggcagc ccctccggca
     3601 agtttccgcc cgaggatgcg ccgccgtggt ggcgtttcag gctgccagca tcctccaatt
     3661 cgcggatgtc gcggcgtatc gtctgcgggg taacgtccaa tgcggcggca agctcgtcca
     3721 ccgacataaa ctgatgccgg cggacaaggc ttaaaatctc tccgtgcctt tggattttcg
     3781 gtttcatcgt tttctgcctc cttgcatcgg gatgccgatt ttaccgcgtt cagcccaaag
     3841 cggaaaacac caccatcaga aacggagcgg cgatattgac caccacgccg aagctgaccg
     3901 ctaccggtac ggcttccaag ccgcccgcac cccgaatcac gggcaatgtg aaatccatac
     3961 tggtcgcgcc gccgaccccc actgccgcat cgggaaaacg cttcatcagc agcggaataa
     4021 atgccagcgc gaacagctct cgtgccaaat cgttcaaaag cgcgatactg ccccataccg
     4081 cgccgtaagc ctcggtcatc accaaacccg agagggaata ccaaccgaag ccggaagcca
     4141 tcgccaaacc tttcacccac gacacaccgt ctgccgatgc ggcaaacagc agcccgcctg
     4201 aaagagatga aagcataaac cagaccgaca accgaatacc cctgcggttg accaaaacct
     4261 gccgcaacga tacgccgctg cttttgagct gtacgccgat gaggagcacc agcagcatca
     4321 gacaatacat acccgcgttt tcagacggca tccaaatatc gcgcatcagt ttgccggatg
     4381 caaatccgag cagcacgcat ccgagctgcc ccacactgcc cgacacaccg accgaaacgc
     4441 ccttcccttt cccctttatc cgccacggga ataactttcc caacactgcc aaagcaagca
     4501 ggttcgcccc gaccgtacaa acaaacagcc acagaaccgt caacgccata tcgtccaacc
     4561 gcgaacccaa atcctccacg cgcgacaacg atacgccgat cagcagcagc acggcataca
     4621 ccaagaccga tagcacctta tccaaagcgg gcaggtaagg cttgggcaca cggataaaaa
     4681 atccggcaaa catcggtatc aataccgaaa gcaatgtcat caggctgtcc atctactgct
     4741 ctcctttatt gccgcatgat atgtgcggtt taaaaattgc cgtctgaaaa ttgcagatac
     4801 ccgcatccat atttcagacg gcatcaggtt cgccattaaa aaaccgcctg aaggttcagg
     4861 cggcttatcc gctccggcat tcaatcttcc aaagtctttt ccaaacgctc catacagttg
     4921 cccaaatggc ggcgcaggat tttgaccacg cggttgcgcc tgcccgccag cagcaggtcg
     4981 aggatttcgc ggtgttcgga atgcgtatgc gtattgatgg cgtgtttttc ctcgcgatgc
     5041 acgcccgcca cggcgacaat cagggaagac cgcgcgcaca gcgtgttcat aatgtcgaac
     5101 agcacatcgt tgcccaccag gcgcgccagt tcgacgtgga aggcattgga caggcggttc
     5161 cagccgacgc ggtcgcccct gccggaagcc tcttcttcgc gccttatcat cgcataaagc
     5221 ggcttgaggc gcgtttccaa atccggcaaa tctgcgagga tgttcaaaat catcgtctcc
     5281 atttcgatgc gcgcattgaa cacatcctgc atttctttca aatcgggaac gtggacgaac
     5341 gcgcccctgt tgggttgcaa atcgacaatc ttgtcgtgcg ccaaaagcga cagcgcgccg
     5401 cggacggtgt tgcgcgaaca caccatctgg cggcaaagtt cggactcggt cagctttttg
     5461 ccgggcagca gcacctgatc ggtaatgccg tccaaaatca gggcgtaaac acggaacagc
     5521 tccgaatcgt gccgctcttc gagaatcagg gaagacgtgg tcggcgcatg gataatgtcg
     5581 tcgttttcaa agttcatgat gttttccgta tttttacgct ttcaaatttt ttaagatgtt
     5641 ttaaggcggc tgtgtttcaa atcgtgtcgg ggaaattaaa gcattgcaca aatttatttt
     5701 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaataattca
     5761 cttggtgctt cagcacctta gagaatcgtt ctctttgagc caaggcgagg caacgccata
     5821 ctggtttttg ttaatccact ataattcaat aaattaatat atggcttaaa ataacaggat
     5881 gcccgccttc cgcccgcccg cagaagcagg cgggtatcat tttaaaacgc ggcatttaaa
     5941 atttgaccga aaattgttga caatccggaa tcaagtctgc acaatacccc gacaagtcca
     6001 agtattataa aggctgaata aagaggaaac agcaggcaga tatattcggg aggtgcagtc
     6061 cgaatatatc tgctttttta tgcgcctccg gcttgcctgc cgcacctttc cccttcagac
     6121 ggcatcagcc gtttctccat aatgccgccc gatgcctatt tatctgcccc ggcaatttca
     6181 aaactgtggg taatctttgc cgctttgccc aacataatcg aagccgaaca gtatttttcg
     6241 gcagacatct gaacggcgcg ctcaatggcc gattctttca aatcatgccc gaatactttg
     6301 aaatggatgt ggatttcggt aaacacgcgc ggcgcatcgt ccgcccgttt cgccgtaacc
     6361 gtcgcacggc agtcagtcac tttctgacgc tgtttttcgg caatcatcac cacatcgatg
     6421 ctcgaacagc ccgccacgcc caacagcagc atttccaaag ggctgggccc gcgcttggcc
     6481 gcaccttctg ccgccgaacc ctccataacg acgctgtgcc cgccttccgt cgtgccgaca
     6541 aagcacattc cgtctatcca ttttgatgta acctgcatgg tgtcattcct gaaaatagcg
     6601 ttaaaaccgc tttgcatatg gcgttattgt aaacaatttc aagcggctta tgcagaaata
     6661 tggacaaaac ggcaaaaaaa cacttgaaaa ccgatttacg gtttggcgga ctggccgttg
     6721 atctgcaccg atttgagttt cagcgtatag gttttgccgt cgtcggtata gccgatttgt
     6781 gccggaatat tgttcaggga cggtgcgaag aaatacatta ccgtatcgtc gccgcgccgc
     6841 acccgatatt tgacgacttc ggtttccacg ccgcctatgc tgtattttcc tgtacccgcc
     6901 ttattcaaac cgccgacgga ataaagtttt ttgccgttgg tgattttcag ccccgagggg
     6961 agtttcgcgt catttgccgc caactgccag gcaagcgtga acaaatccat agccttgggg
     7021 ctttgctcgg ttttgctctc gcccgctttg ccgtaggtta cgctgccgtc ggcgaatttg
     7081 gcttccgcat acagtttgcc cctgcgtatg tctctatagt aggtagggtg cagggtattg
     7141 ccgacaaccg taccgccgga ctcgaaacgg atattgtata gcggcacttt aatcgtcgaa
     7201 acgattttgt aagcattgcc gctgcgttca aatgtcatcg tggcgggaat gccgtagctg
     7261 ccggaatagt gcagcacggc ggattggggc agccctgccg catacgcgca cggcagggcg
     7321 gcggacaaaa tggcggcgga aaatatattt ttaaaagtct tcatcatttg ctcccgcccg
     7381 gtttacgccg tcaggaaacg ggcggcatcg gcgttttccg aatttctgac gcggtttccc
     7441 tcgataatca ggcggccggc ggcaaaatcg gcaacggctt tcggataaag tttatgctca
     7501 acagccaaaa cccgggcggc aacatcgtct gccgtatcgc cgtcgagtat cggcacaatc
     7561 ccttgcgata caatcgggcc gcaatccagt tcggcagtaa cgaaatggat ggtgcagccg
     7621 gcaacgcggc agcccgcctc caaagcgcgt tcgtgcgtat gaagtccggt aaacgagggc
     7681 aggacggacg ggtgaatgtt catcagcctg ccttcgtaat gggcacagaa ctcaggggtc
     7741 agaatccgca taaaacctgc caaaaccacc aagtcgggtt gatatgcgtc gattttttcc
     7801 atcatggcgg tatcgaaggc aagtcgtgat tcaaagtttt tatgattcag gctgtcggtc
     7861 ggaataccct gaccggccgc ccattgcaaa ccggcagccg tttcgctgtt gctcaacacg
     7921 gcggcaatac ggacgttggg aatggcggca ttgacgattg cctgcatatt gctgccgcgt
     7981 ccggaaatca ggatgacgat gtttttcata atggtgcgct tttgaaaggg atgccatctg
     8041 aaccgctgtt tggtggtttc agacggcatt tgccgtaaaa atgcccgaaa acctgtttcg
     8101 ggcatggatt cggacttaat ttactttttt gatgtcgact tgagccggct gcttggcggg
     8161 cgcgttttcg ggtgcgccga ttttgaccag tttcacatca aataccaaag tggcgttcgg
     8221 gccgattttg tcgcccgcac cctgttcgcg gtaggcaagg ttggacggga tgtagaacgt
     8281 ggcttcgccg ccttctttca gaagctgtac gccttcggtc caacccggaa tcacttggct
     8341 caaagggaag gtgaccgggc cgccgttggc tttgctgctg tcgaataccg taccgtcaat
     8401 caggcggcct tcgtattcca cggtaacgat gtcgtctttg gtcggctgtt tgccttcgcc
     8461 ctgtttggtg attttgtatt gcaggccgga agcagtggtc ttcacgccgt ctttggcggc
     8521 attttctttc agaaaggctt cgcctttttc tttattggcc ttcgcgtccg ccttgtgttt
     8581 ttctacggct ttagcctgtt gttcctgaag gaatttcatc atgacttcct gagcctgctc
     8641 ttcagtcatt ttgatttctt tgccgtcata cactgcctgc atggcttcgg taaagacttt
     8701 caaatcgatt tccgcaccct gttccttcat ttgcttcagg gagcgtccga tgtccacgcc
     8761 catcgcatag cttgcctgct gcatcgtgct gccgatcgaa gaggtgtcgc cctgcgcggc
     8821 agaagcggcg gcaggttcgg atgcagatgc gggggcggct tcttttttgc cgcaggcgga
     8881 aagtgccaaa gcggcggaaa gggtcagtgc gctgattttg aaaatggtgt tcatgatgga
     8941 tcttcgctgt cgataaggtc ggaaaaacgg gattatagcc gagtttgaat gtttcaacac
     9001 acaggatgac acataaagcg tcaatcgtgt gttgccctgt tttggaaggg attgaacctt
     9061 ccaaaataag ttttgattct accgccccga gggacagatg tccaagtggc ggggttcaac
     9121 cgataaggaa attttaatca aatagaatca agcctgttta aattttgtaa atgcggcatt
     9181 tcagacggca ttttatgcct tgccctccat gccgtgatgt tcgatggcaa aaccgcttcg
     9241 gcggtaggcg gtaaagcgtt cgcgcgcgtc ggcgagatct tccaagctgt tgccgacgat
     9301 ttccaaaacg cgcgcgggca agaccggagc ggtgttccaa aaaccgtcgg acaggttcaa
     9361 aacagtcatg ccttcgggaa ttcggggcgg attgccgccg caagcaagca ggacggatgt
     9421 ttcagacggc atggcttctt ccatttccca aatttcgtgc ggaatgaaac tctcggcttc
     9481 gtattgccaa agcattttgt ccaattcctg aagccgcccg aacgaatcgg accacaccag
     9541 tatcctgccg ccgtcccgaa tcgcacgggc aatcaggcgg caggtgaaaa tcggaacttg
     9601 ggcaacgtgc gtgtaaaagg tggctttcgg catattgttt gagcatttgg caggacgatg
     9661 ccgtctgaaa ggcttcagac ggcattgtgg caaaattaaa gattccgcag atagttcagc
     9721 agcaagggaa cgggacggcc agtcgcacct ttttccgctc cggatttcca cgccgtaccc
     9781 gcgatgtcaa ggtgcgccca cgggtagcct tcggtgaagt aagacaggaa tgttgcggcg
     9841 gtaatcgtgc ccgcgccggg cgtgccgatg tttggaatgt cggcaaagtt ggatttgagt
     9901 tggtctttgt aggtctcaaa gagcggcagt tgccatgctt tgtcgtccac gttgtaggaa
     9961 gcggcaagca ggctgtcgat caaatcctga ttgttgccca tcacgccgct gacatcgtgc
    10021 cccaaggcaa caatacacgc gccggtcagg gtggcgacgt cgatgacggc tttgggtttg
    10081 aactgctcgg cgtaagtgag cgcgtcgcac aaaatcagac ggccttcggc atcggtgttc
    10141 aacacttcga tggtcagacc tttcatactt ttcacgacat cgcccggttt gtttgccgcg
    10201 ccggaaggca tattttcaca agtggcgacg acggcaatca ggttaatcgg cagttgcagt
    10261 ttgacggcgg cgcagaaggt gctgatgacg gttgccgcgc cgcacatatc aaacttcatt
    10321 tcgtccatgt tcaggccggg cttgagggag atgccgccgg tgtcgaaggt aatgcctttg
    10381 ccgaccaata ccacaggcgc ggcttctttg tcggctgcac cgaaatagct cagttcgacc
    10441 aaataggggg cttccgcgct gcctttggcg accgaccaaa acgaacccat gttttctttg
    10501 atgtagtctt tttcgatgat tttggcgtgc gcgcccagtt tttcggcttc ggctttggcg
    10561 gtgcgcgcta aaaattcggg cgtgcattcg ttgggcgcgg cgttgcccaa gtcgcggcag
    10621 aggctttgtc cgtaaacttg cgcttcggcg acgcgcaagg cttctttaac gacggcttcg
    10681 tgtgcgctgt ggaacacggc ggtttcaaat ttggcgggct tggcttcttt tttgtagcgg
    10741 tcgaaacggt aggcggcgtt gccgaacgca atcgcaaacg cttcggctac ggcggcggct
    10801 tgcgcttcat cgaaggcatg gatgtccaca ttgaccgttt cctgattttg cgcccatttg
    10861 gcggcttcgg cggcggcctt gttcaatgcg gcgcggccgg tgcttttcag acagcatacg
    10921 gcaacagcct gcaaaccgtt gcctgtcggg atttttgtgt cggcaaaatt ttgaccttct
    10981 tcaagcgaag acaaaagggc aagggcggtc ggatggttta attgcgcctc ttcggtgcag
    11041 acaaataact gtgcgcctgc ctgctgttcc tgcaagattt cggtttttgt gctaaattcc
    11101 acgtttattc tcctgattga gacggttgtc ggtagttttc ggacggcctt tcgctcaaaa
    11161 gaccgtctga agacggctgg cacgattgta ccccatttga agcaccgtct gaaaccttgc
    11221 gcggacaatc cgcctgcgcc gaaccgctta ccgccccctg accgcgattc tatgatttat
    11281 caaagaaacc tcatcaaaga actctctttt accgccgtcg gcattttcgt cgtcctcttg
    11341 gcggtattgg tctccacgca ggcaatcaac ctgctcggcc gtgccgccga cgggcgtgtc
    11401 gccatcgatg ccgtgttggc attggtcggc ttctgggtca tcggtatgac gccgcttttg
    11461 ctggtgttga ccgcatttat cagtacgttg accgtgttga cccgctactg gcgcgacagc
    11521 gaaatgtcgg tctggctatc ctgcggattg gcattgaaac aatggatacg cccggtgatg
    11581 cagtttgccg tgccgtttgc cgttttggtt gccgtcatgc agctttgggt gataccgtgg
    11641 gcagagctac gcagccgcga atacgctgaa atcctgaagc agaagcagga attgtctttg
    11701 gtggaggcag gcgggttcaa cagtttgggc aagcgcaacg gcagggttta ttttgtcgaa
    11761 accttcgata ccgaatccgg catcatgaaa aacctgttcc tgcgcgaaca ggacaaaaac
    11821 ggcggcgaca acatcatctt cgccaaagaa ggtaacttct cgctgaacga caacaaacgc
    11881 acgctcgaat tgcgccacgg ctaccgttac agcggcacgc ccggacgcgc cgactacaat
    11941 caggtttcct tccaaaaact caacctgatt atcagcacca cgccaaaact catcgacccc
    12001 gtttcccacc gccgtaccat tccgaccgcc caactgattg gcagcagcaa cccgcaacat
    12061 caggcggaat tgatgtggcg catctcgctg accgtcagcg tcctcctact ctgcctgctt
    12121 gccgtgccgc tttcctattt caacccgcgc agcggacata cctacaatat cttgattgcc
    12181 atcggtttgt ttttaattta ccaaaacggg ctgaccctgc tttttgaagc cgtggaagac
    12241 ggcaaaatcc atttttggct cggactgctg cctatgcaca tcatcatgtt cgtcatcgca
    12301 atcgtacttc tgcgcgtccg cagcatgccc agccagccct tctggcaggc ggttggcaaa
    12361 agtctgacat tgaaaggcgg aaaatgaacc tgatttcacg ttacatcatc cgtcaaatgg
    12421 cggttatggc ggtttacgcg ctccttgcct tcctcgcttt gtacagcttt tttgaaatcc
    12481 tgtacgaaac cggcaacctc ggcaaaggca gttacggcat atgggaaatg ctgggctaca
    12541 ccgccctcaa aatgcccgcc cgcgcctacg aactgatgcc cctcgccgtc cttatcggcg
    12601 gactggtctc tctcagccag cttgccgccg gcagcgaact gaccgtcatc aaagccagcg
    12661 gcatgagcac caaaaagctg ctgttgattc tgtcgcagtt cggttttatt tttgctattg
    12721 ccaccgtcgc gctcggcgaa tgggttgcgc ccacactgag ccaaaaagcc gaaaacatca
    12781 aagccgcggc catcaacggc aaaatcagta ccggcaatac cggcctttgg ctgaaagaaa
    12841 aaaacagcat tatcaatgtg cgcgaaatgt tgcccgacca taccctgctg ggcattaaaa
    12901 tctgggcccg caacgataaa aacgaactgg cagaggcagt ggaagccgat tccgccgttt
    12961 tgaacagcga cggcagttgg cagttgaaaa acatccgccg cagcacgctt ggcgaagaca
    13021 aagtcgaggt ctctattgcg gctgaagaaa actggccgat ttccgtcaaa cgcaacctga
    13081 tggacgtatt gctcgtcaaa cccgaccaaa tgtccgtcgg cgaactgacc acctacatcc
    13141 gccacctcca aaacaacagc caaaacaccc gaatctacgc catcgcatgg tggcgcaaat
    13201 tggtttaccc cgccgcagcc tgggtgatgg cgctcgtcgc ctttgccttt accccgcaaa
    13261 ccacccgcca cggcaatatg ggcttaaaac tcttcggcgg catctgtctc ggattgctgt
    13321 tccaccttgc cggacggctc ttcgggttta ccagccaact ctacggcatc ccgcccttcc
    13381 tcgccggcgc actacctacc atagccttcg ccttgctcgc cgtttggctg atacgcaaac
    13441 aggaaaaacg ctaaaccaat gccgtctgac ccccttcgga cggcattggt ttttattatc
    13501 atattcctgt agacagatag ccgttcccta ttatagtgga ttaacaaaaa ccagtacggc
    13561 gttgcctcgc cttgccgtac tattgtactg tctgcggctt cgtcgccttg tcctgatttt
    13621 tgttaatcca ctatacatta cctgtcataa cagttccatt tttgttaaaa ctagtctatg
    13681 atagcggtac aaatattgtt tacaatattt aacgcaaatc atttgcaacc cgacaaaaga
    13741 aaaacagaaa aaggaacaaa gagatgttag aagcctatcg taaagccgcc gccgagcgcg
    13801 ccgccctcgg cattcccgcc ctccctttga acgcgcagca aaccgccgat ttggttgagc
    13861 tgctgaaaag cccgcccgca ggcgaaggcg agttcttggt cgaactgctt gcccaccgtg
    13921 ttccgcccgg tgtggacgat gccgccaaag tcaaagcctc attcctggct gccgttgccg
    13981 aaggcagcgc gtccagcccg ctgatctccc ccgaatatgc gaccgaactc ttaggtacga
    14041 tgctcggcgg ttacaatatc cacgccttaa tcgaactctt ggacgacgac aaactcgcgc
    14101 ccattgccgc caaaggcttg aaacatacgc ttctgatgtt cgattccttc cacgacgttc
    14161 aagaaaaagc cgaaaaaggc aacaaatacg cgcaagaagt tttgcaatct tgggcagatg
    14221 ccgaatggtt cgcctcacgc gccaaagttc ccgaaaaaat caccgttacc gttttcaaag
    14281 ttgacggcga aaccaataca gacgacctct cccccgcgcc cgacgcgtgg agtcgtcccg
    14341 atattccgct gcacgcgctg gcaatgctga aaaacccgcg cgacggcatc acgcccgaca
    14401 aaccgggcga agtcggtccg attaaattgt tggaagaact caaagccaaa ggccatccgg
    14461 ttgcttacgt cggcgacgtg gtcggtactg gttcttcacg caaatccgcg accaactccg
    14521 tcatttggca taccggcgaa gacattccgt tcgtgccgaa caaacgcttc ggcggcgtat
    14581 gtttgggcgg caaaatcgcg ccgattttct tcaataccca agaagattcc ggcgcgctgc
    14641 cgattgaagt cgatgtatct gctctaaaaa tgggcgatgt cgtcgatatc ctgccttatg
    14701 aaggcaaaat cgtgaaaaac ggcgagactg ttgccgagtt tgaattgaaa tcacaagtat
    14761 tgctggacga agtgcaagcc ggcggccgta tcaacctgat tatcggccga ggtctgaccg
    14821 ccaaagcgcg cgaagccctg aaactgcctg cctctactgc attccgcctg ccgcaagcgc
    14881 ctgccgaaag caaagccggt ttcaccttgg cgcaaaaaat ggtcggccgc gcctgcggtc
    14941 tgcccgaagg acaaggcgtg cgcccgggta cttactgcga accgcgtatg acgacggtcg
    15001 gctcgcaaga cacgaccggc ccgatgaccc gcgacgagtt gaaagacttg gcttgtttgg
    15061 gcttctccgc cgatatggtg atgcagtctt tctgccacac cgccgcctat ccgaaacctg
    15121 tcgatgtaaa aacccataaa gaactgcccg cctttatttc cacccgtggc ggcgtgtcac
    15181 tgcgtccggg cgacggcgtc atccactcgt ggctcaaccg cctgctgctg cccgataccg
    15241 tcggcaccgg cggcgacagc catacccgtt tccccatcgg tatttccttc cccgccggct
    15301 ccggcttggt tgcctttgcc gccgcaacgg gcgtaatgcc gctcgatatg cccgagtctg
    15361 tattggtacg cttcagcggc aagctgcaac cgggcgtaac cctgcgcgat ttggtgaacg
    15421 ccatcccgct gtacgcaatt aaacaaggtt tgctgaccgt tgccaaagcc ggtaagaaaa
    15481 acatcttctc tggccgcatc ctcgaaatcg aaggcctgcc cgatttgaaa gtggaacaag
    15541 cctttgaatt gaccgacgca tccgccgaac gctccgccgc cggctgtacc gtgaagctca
    15601 ataaagagcc gattatcgag tacatgaaat ccaacgtcgt gttgatgaaa aacatgattg
    15661 ccaacggcta tcaagacccg cgcactttgg aacgccgcat caaagccatg gaaaaatggc
    15721 tggcaaatcc cgagttgctc gaagcggata aagatgccga atacgccgcc gtgattgaaa
    15781 tcaacatgga cgacatcaaa gagccgatta tcgcctgccc gaacgacccg gacgacgtgt
    15841 gcttcatgtc cgaacgctcc ggcaccaaaa tcgacgaagt attcatcggt tcgtgtatga
    15901 ccaacatcgg ccacttccgc gccgcctcca aacttttgga aggcaaggca gacacccccg
    15961 tccgcctgtg gattgcgccg ccgaccaaaa tggacgcgaa acaattgtcc gacgaaggac
    16021 actacggcgt actcggacgt gccggcgcgc gtatggaaat gccgggttgc tccttatgta
    16081 tgggtaatca ggcgcaagta cgcgaaggtg cgaccgttat gtccacctcc acccgcaact
    16141 tcccgaaccg tttgggtaaa aacacctttg tttacctcgg ttcggcggaa ttggcagcga
    16201 tttgctccaa actgggtaaa atcccgaccg ttgaagaata tcaagccaat atcggcatca
    16261 tcaacgaaca gggcgataaa atctaccgct atatgaactt caacgaaatc gacagctaca
    16321 acgaagtagc cgagaccgtg aacgtttaat ccccgtcatc cgtatgaagt aagggattga
    16381 ccgcaatgcc gtctgaacaa ccttcagacg gcattgcaac attccgctaa cccttctttc
    16441 cgcaaacgct gcaaatacgg cgttcacgcc cccacataaa ggaaacgaca gtgaacctga
    16501 aaaaccgcca ttttctgaaa cttttagact tcacgccgga agaaatcacc gcctacctcg
    16561 accttgccgc cgaattgaaa gccgccaaaa aagcagggcg cgagattcag cggatgaaag
    16621 ggaaaaacat cgccctgatt tttgaaaaaa cctctactcg gacgcgctgc gcgtttgaag
    16681 tcgccgcgcg cgatcaaggc gcgggagtga cttatttaga gccgtccgcc agccaaatcg
    16741 ggcataagga aagcatcaaa gacaccgccc gcgtgttggg caggatgtac gatgccatcg
    16801 aatatcgcgg tttcggtcag gaagttgttg aagaattggc gaaatacgcg ggcgtacccg
    16861 tgttcaacgg gctgaccaac gagttccatc ccacacaaat gcttgccgac gcactgacta
    16921 tgcgcgaaca cagcggcaaa cctttgaacc aaaccgcgtt tgcctacgtc ggcgacgcgc
    16981 gttacaacat gggcaattcc ctgctgattt taggggcaaa attggggatg gacgtgcgta
    17041 tcggcgcacc gcaaagcctg tggccgtctg aaggcattat tgccgccgca cacgccgccg
    17101 ccaaagaaac cggcgcaaaa attaccctga ccgaaaacgc gcatgaagcc gtgaagaatg
    17161 ttgattttat tcataccgat gtgtgggtca gcatgggcga gccgaaagaa gtctggcagg
    17221 aacgcatcga tttgctgaaa gattaccgcg ttacgcccga actgatggcg gcatcgggca
    17281 atccgcaagt caaattcatg cactgcctgc ccgccttcca caaccgcgaa accaaagtcg
    17341 gcgaatggat ttacgaaacc ttcgggctga acggtgtgga agttacagaa gaagtattcg
    17401 aaagccccgc cagcatcgtg ttcgatcagg cggaaaaccg tatgcacacg attaaagcgg
    17461 taatggtcgc ggctttgggc gactgacaga actgtgcctg tttaaattca tccgcaacac
    17521 agataccgtc tgaacacgat gttcagacgg tatcca
//
LOCUS       DAWKQJ010000028        17308 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000028 DAWKQJ010000000
VERSION     DAWKQJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.028"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..1165
                     /gene="tuf"
                     /locus_tag="WJL20_000702"
     CDS             32..1165
                     /gene="tuf"
                     /locus_tag="WJL20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7682880.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            1172..1247
                     /locus_tag="WJL20_000703"
     tRNA            1172..1247
                     /locus_tag="WJL20_000703"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1205..1207,aa:Trp,seq:cca)
     gene            1362..1640
                     /gene="secE"
                     /locus_tag="WJL20_000704"
     CDS             1362..1640
                     /gene="secE"
                     /locus_tag="WJL20_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ7682881.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            1642..2178
                     /gene="nusG"
                     /locus_tag="WJL20_000705"
     CDS             1642..2178
                     /gene="nusG"
                     /locus_tag="WJL20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ7682882.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQK
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            2279..2713
                     /gene="rplK"
                     /locus_tag="WJL20_000706"
     CDS             2279..2713
                     /gene="rplK"
                     /locus_tag="WJL20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ7682883.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            2713..3408
                     /gene="rplA"
                     /locus_tag="WJL20_000707"
     CDS             2713..3408
                     /gene="rplA"
                     /locus_tag="WJL20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ7682884.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            3638..4138
                     /gene="rplJ"
                     /locus_tag="WJL20_000708"
     CDS             3638..4138
                     /gene="rplJ"
                     /locus_tag="WJL20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ7682885.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            4196..4567
                     /gene="rplL"
                     /locus_tag="WJL20_000709"
     CDS             4196..4567
                     /gene="rplL"
                     /locus_tag="WJL20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ7682886.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            4757..8935
                     /gene="rpoB"
                     /locus_tag="WJL20_000710"
     CDS             4757..8935
                     /gene="rpoB"
                     /locus_tag="WJL20_000710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ7682887.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            9092..13267
                     /gene="rpoC"
                     /locus_tag="WJL20_000711"
     CDS             9092..13267
                     /gene="rpoC"
                     /locus_tag="WJL20_000711"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ7682888.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            13558..13815
                     /locus_tag="WJL20_000712"
     CDS             13558..13815
                     /locus_tag="WJL20_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682889.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            13812..13961
                     /locus_tag="WJL20_000713"
     CDS             13812..13961
                     /locus_tag="WJL20_000713"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7682890.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKISSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            14140..14511
                     /gene="rpsL"
                     /locus_tag="WJL20_000714"
     CDS             14140..14511
                     /gene="rpsL"
                     /locus_tag="WJL20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ7682891.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            14629..15099
                     /gene="rpsG"
                     /locus_tag="WJL20_000715"
     CDS             14629..15099
                     /gene="rpsG"
                     /locus_tag="WJL20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ7682892.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            15118..17223
                     /gene="fusA"
                     /locus_tag="WJL20_000716"
     CDS             15118..17223
                     /gene="fusA"
                     /locus_tag="WJL20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ7682893.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4591 a         3908 c         4494 g         4315 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggtgccgc acaaatggac ggtgcaatct tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ctgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 ggaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca aattcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc gctttgcgat tcgcgaaggc ggtcgtaccg tgggtgccgg
     1141 cgtggtttct tctgttatcg cttaagttta gaggccaata gctcaattgg tagagtatcg
     1201 gtctccaaaa ccgagggttg ggggttcgag accctcttgg cctgccaaat aaaaaattaa
     1261 ccggccttgt gtcggttaat ttttttgtat ttgttattta gtaaactctc ttgccattta
     1321 catggattga gaatagacag atgctatgat ggataaataa tatgacagaa catacgcctg
     1381 aaaaaaagaa cgttaaagtg gatcaactgg ttgttcaaga taaagaatct gcatctaatt
     1441 ccggtaagga agggtttttt gcatatttct caaattcttg gtccgaattc aaaaaggtgg
     1501 tttggcctaa gcgtgaagat gctgtcagaa tgactgtatt tgttatagtg tttgttgctg
     1561 tgctttctat atttatctat gcggcagata cagcaatttc gtggttattt tttgatgtat
     1621 tgctgagaag ggaaggttga gatgtcgaaa aaatggtatg ttgtacaggc gtattcgggg
     1681 tttgagaaga atgtccaacg aatattggaa gagcgcattg cccgtgagga gatgggagat
     1741 tatttcggac aaattctggt gcctgtagag aaagttgttg atatccgcaa tggtcgtaag
     1801 actattagtg aaagaaagtc atatcctggt tatgtgctag ttgagatgga aatgacagat
     1861 gactcttggc atcttgtaaa aagcaccccc cgtgtttccg gttttattgg agggagggct
     1921 aatagaccta cgccgattag tcagaaagag gctgaaatta ttttacagca ggttcagacc
     1981 ggcatagaga agccgaaacc aaaagttgaa tttgaggtcg gtcaacaggt tcgtgtaaat
     2041 gaagggccgt ttgcggattt taacggggtg gttgaggagg tcaattatga acggaataag
     2101 ttacgcgtgt ctgttcagat atttggtaga gaaacacccg ttgagctgga gttcagccag
     2161 gttgaaaaga ttaactgatt tttatacttg aaaaaaaagc aataagagga tagaatcaaa
     2221 aattaacttg gggagcggaa atggttccgc gtcttacccg tttttaggag ttcgttaagt
     2281 ggcaaagaaa attatcggct atattaaact gcaaattcct gcaggtaaag ccaatccatc
     2341 tcctccggtt ggtcctgctt tgggtcagcg cggtttgaat attatggaat tttgtaaggc
     2401 atttaatgct gcaacccaag gtatggagcc tggcttaccg attccggttg tgattactgc
     2461 atttgcagat aaatcattca catttgtgat gaaaaccccg ccagcttcta tcttgttgaa
     2521 aaaggctgcc ggtttgcaaa aaggtagttc taatcctctg accaacaaag tgggtaaatt
     2581 gacccgtgcc cagttggaag aaattgctaa aactaaagat cctgatttga ctgctgctga
     2641 cttggatgcg gctgtccgta ctatagcagg ttctgctcgc tcaatgggct tggatgtgga
     2701 gggtgttgta taatggctaa agtatctaaa cgcttgaaag ctcttcgctc ttctgtggaa
     2761 gccaataaat tatatgcaat tgatgaagca attgctttgg taaaaaaagc agcgactgct
     2821 aaatttgacg agtctgttga cgtatctttc aacttgggcg ttgatccgcg taaatctgac
     2881 caagttatcc gtggatcggt cgttctgcct aaaggcaccg gtaagataac ccgtgtggct
     2941 gtatttactc aaggtgcaaa tgcagaagct gctaaagaag ctggtgcaga tatcgtcggt
     3001 ttcgaagatt tggctgctga aatcaaagcg ggcaatctga actttgacgt tgttattgct
     3061 tctcccgatg caatgcgtat tgttggtcaa ttgggtacca tcttgggtcc tcgtggtctg
     3121 atgccaaacc ctaaagtagg tacggttacc cctaatgttg ccgaagcagt aaaaaatgca
     3181 aaagcaggtc aagtgcagta ccgtaccgac aaggcaggta ttgttcatgc aacgattggt
     3241 cgtgcttcat tcgctgaagc tgatttgaaa gagaactttg atgcgttgct ggatgctatc
     3301 gttaaagcca agcctgctgc cgctaaaggt cagtatctga aaaaagttgc tgtgtctagc
     3361 accatgggtt tgggtattcg cgttgataca tcaagcgtaa ataactaatc ttaaggaatt
     3421 ttcaagcagt ttggttttct gggctgcttg aatttgggct acttaaaatt aagtagatgt
     3481 ccaagaccgt agggatcgta agatttaatc gtaactgccc tacgcagacg gtagtcctga
     3541 aacacattgc aagattgctt gtaagatgtc tttttaggtt accgcgctgg tgggatatcg
     3601 ttttggtatc ctgtttataa acagtgggag gtagaccttg agtctcaata ttgaaaccaa
     3661 gaaagtggcg gtcgaggaaa ttagcgcggc aattgctaat gctcaaaccc tcgtagtcgc
     3721 tgaatatcgc ggtatcagtg tttccagtat gactgagctt cgtgcgaatg cacgtaaaga
     3781 aggcgtttat ttgcgtgttc tgaaaaatac tttggctcgt cgtgcagtgc aaggtacttc
     3841 atttgcagaa ttggccgatc aaatggttgg tccgttggtt tacgctgctt ctgaagatgc
     3901 tgttgctgct gctaaagtgt tgcaccaatt cgcgaaaaaa gatgacaaaa ttgtcgttaa
     3961 agccggttct tacaatggcg aagtaatgaa tgctgctcag gttgctgagt tggcttctat
     4021 tccgagccgc gaagagctgt tgtccaaact gttgttcgtt atgcaagctc ctgtatcggg
     4081 ctttgcgcgc ggtttggctg ctttggcaga gaaaaaagcc ggcgaagaag ccgcttaatc
     4141 gattttgttt ctgttaatca attatttttt aatacaatat ttggagtaaa atagcatggc
     4201 tattactaaa gaagacattt tggaagcagt tggttctttg accgtaatgg aattgaacga
     4261 cttggttaaa gcttttgaag aaaaatttgg tgtttctgct gctgctgttg cagttgcagg
     4321 tcctgctggt gccggtgctg ccgatgctga agaaaaaacc gaatttgatg tcgttttggc
     4381 ttctgccggc gatcaaaaag tcggcgtgat taaagttgtc cgtgcaatta ccggtttggg
     4441 tctgaaagaa gctaaagaca tcgttgacgg cgcacctaaa accattaaag agggtgtttc
     4501 taaagctgaa gccgaagaca tccaaaaaca actggaagaa gcaggcgcta aagtcgaaat
     4561 caaataattt gatgcttctt atgaaggctg gcagttttct gccagcctta ttttgcttct
     4621 taaaataaac atcaagtatt gtttacattt atttgcatag tttttatcaa gtcattgcaa
     4681 ataaatgtaa atatcagatt gatgcgtacc gttgtttcag acggcctatt attgaaaatt
     4741 acttttcgga gtgtgtatga actattcgtt taccgagaaa aaacgtatcc gtaagagttt
     4801 tgcaaagcgg gaaaatgttt tggaagttcc tttcctgcta gcaactcaga ttgattctta
     4861 tgcgaaattt ttgcaactgg aaaatgcttt tgacaaacgt accgatgacg gtctgcaggc
     4921 ggcatttaat tctattttcc cgattgtgag ccataacggt tatgcgcgat tggagtttgt
     4981 gcattacaca ttgggcgagc ctttgttcga tattcccgaa tgtcagttgc gcggaatcac
     5041 ttatgcagcc cccttgcgcg cgcgtatccg tttggtgatt ttggataagg aagcatctaa
     5101 accgacggta aaagaagttc gtgaaaacga agtgtatatg ggcgaaattc cgttgatgac
     5161 cccgagcggt tcttttgtga ttaacggcac agagcgtgtg attgtctccc agttgcaccg
     5221 ttcgcccggc gtattcttcg agcatgacaa aggtaagacg cactcttccg gcaagttgtt
     5281 attctccgcc cgtatcattc cctaccgcgg ttcatggttg gattttgaat ttgacccgaa
     5341 agatttgctg tatttccgta tcgaccgccg ccgtaaaatg ccggtaacga ttttgttgaa
     5401 ggctttgggc tacggcaatg agcaaatctt ggatattttc tacgacaaag aaacgttcta
     5461 tttgtcttca aacggtgttc aaaccgattt ggtcgcaggc cgtctgaaag gcgaaactgc
     5521 caaggtcgat atcttggata aagaaggcaa tgtattggtt gccaaaggta agcgcattac
     5581 tgcgaaaaat atccgtgata ttaccaatgc aggcctgacc cgtttggatg tagaaccgga
     5641 aagcctgctg ggtaaagcat tggctgccga tctgattgat tcggaaaccg gcgaggtatt
     5701 ggcttctgcc aatgatgaaa ttacagaaga gttgttggcc aaatttgata tcaacggcgt
     5761 aaaagaaatt acgacccttt acatcaatga gttggatcag ggtgcttata tctccaacac
     5821 cttgcgtacg gatgagactg ccggccggca ggcggcgcgt gttgcgattt accgtatgat
     5881 gcgtccgggc gaaccgccca ccgaagaggc ggtcgagcaa ttgtttaacc gcttgttctt
     5941 cagtgaagac agctacgatt tgtcccgcgt aggtcgtatg aaatttaata cgcgcacata
     6001 cgaacaaaaa ctgtccgaag cccaacaaaa ctcttggtac ggccgcctgc tgaacgaaac
     6061 gtttgccggt gctgccgaca aaggcggtta tgtcctgagc gtcgaagata ttgtcgcctc
     6121 gattgcgact ttggtcgagt tgcgtaacgg ccatggcgaa gtggacgata tcgatcactt
     6181 gggcaaccgc cgagtacgtt cggtaggcga gctgactgaa aaccaattcc gtagcggttt
     6241 ggcccgtgtg gaacgtgccg taaaagaacg tttgaatcag gcggaatcag aaaacttgat
     6301 gccgcacgat ttgattaatg caaaacctgt ttctgccgct attaaagaat tcttcggctc
     6361 cagccaattg agtcagttta tggatcagac caatcccttg tctgaagtaa cccataaacg
     6421 ccgcgtatcc gcattgggtc cgggcggttt gacccgcgaa cgtgcaggat ttgaggtgcg
     6481 ggacgtgcat ccgacccact acggtcgcgt atgtccgatt gaaacgcctg aaggtccgaa
     6541 catcggtttg atcaactcat tgtccgttta tgcgcgcacc aatgattacg gtttcttgga
     6601 aacgccttac cgccgcgtta tcgacggcaa agtaaccgag gaaatcgatt acttgtctgc
     6661 catcgaagaa ggccgctatg tgattgcaca ggcgaatgcc gatttggatt cagatggcaa
     6721 tctgattggc gatttggtta cctgtcgtga aaaaggcgaa accattatgg caacgcccga
     6781 ccgcgtccaa tatatggacg tggcaactgg tcaagtggta tccgttgcag catccctgat
     6841 tccgttcttg gaacatgatg acgcgaaccg cgcattgatg ggtgccaaca tgcaacgtca
     6901 ggcagtgcct tgcttgcgtc ctgaaaaacc gatggtcggt accggtatcg agcgttccgt
     6961 tgccgttgac tctgctactg caatcgttgc ccgccgaggc ggcgtggtcg agtatgtcga
     7021 tgccaaccgt gttgtgattc gtgtccatga cgacgaagcg actgccggcg aagtgggtgt
     7081 cgatatttac aacttggtta aatttacccg ttccaatcaa tctactaaca tcaaccaacg
     7141 tccggctgtc aaagcaggcg atgttttgca acgcggcgat ttggtggccg atggcgcgtc
     7201 caccgatttg ggcgaattgg ctttgggtca aaatatgacc atcgccttca tgccgtggaa
     7261 cggttacaac tacgaagact cgattctgat ttccgaaaaa gtggctgcgg acgaccgcta
     7321 tacttcgatt cacattgagg aattgaatgt cgttgcccgc gataccaagc tgggtgcgga
     7381 agacattacc cgcgatattc cgaacttgtc cgagcgtatg caaaaccgtt tggacgaatc
     7441 cggtatcgtt tacatcggtg cggaagtaga agccggcgat gtgttggtag gcaaggtaac
     7501 gcctaaaggc gaaacccagc tgacgccgga agaaaaactg ctgcgcgcca tcttcggtga
     7561 aaaagcatct gacgtaaaag atacttcatt gcgtatgcct accggcatga gcggtaccgt
     7621 tatcgacgtt caagtcttca ctcgtgaagg tattcaacgc gacaaacgtg ctcaatccat
     7681 tatcgattcc gagttgaaac gctaccgttt ggatttgaac gaccaattgc gtattttcga
     7741 caacgacgca ttcgaccgta tcgagcgtat gattgtcggt cagaaagcca acggcggccc
     7801 gatgaagctg gccaaaggca gtgaaatcac gaccgaatat ctggcgggtc tgccgagcag
     7861 gcacgattgg ttcgatatcc gtctgaccga tgaagatttg gccaagcagt tggaactgat
     7921 taaagtgagc ctgcaacaaa aacgcgaaga agcggacgag ttatacgaaa tcaagaagaa
     7981 aaaactgacc caaggcgacg aattgcaacc cggcgtacaa aaaatggtga aagtttttat
     8041 cgccatcaaa cgccgtctgc aagccggcga caaaatggcg ggccgccacg gtaacaaagg
     8101 cgtggtatcg cgcattctgc cagtggaaga catgccttac atggcggacg gccgtccggt
     8161 agacatcgta ctgaacccat tgggcgtacc ttcccgtatg aacatcggtc agattttgga
     8221 agttcacttg ggttgggcag caaaaggtat cggcgagcgt atcgaccgta tgctgaaaga
     8281 gcaacgcaaa gcaggcgagt tgcgcgagtt cttgaacaga ctctacaacg gcagcggcaa
     8341 gaaagaagat ttggatgccc tgactgatga agaaatcatc gaactggcct ccaacctgcg
     8401 taaaggtgca tctttcgcct ctcctgtatt cgacggtgcg aaagagtctg aaatccgcga
     8461 aatgttgaac ttggcttatc cgagcgacga tcctgaggtt gaaaaactgg gcttcaacga
     8521 cagtaaaacc caaatcacgc tgtatgacgg ccgttcaggc gaagcatttg accgcaaggt
     8581 tacagtaggt gtgatgcact atctgaaact gcaccacttg gttgacgaaa aaatgcacgc
     8641 gcgttctacc ggtccgtaca gtctggttac ccagcagcct ttgggcggta aagcccagtt
     8701 cggcggccaa cgtttcggcg agatggaggt ttgggcattg gaagcatacg gcgcggcata
     8761 cacgctgcaa gagatgctga ctgtgaagtc tgacgacgtg aacggccgta ccaaaatgta
     8821 cgaaaacatc gtcaaaggcg aacacaaaat cgatgccggt atgcccgagt ccttcaacgt
     8881 attggtcaaa gagattcgct cactgggctt ggatatcgat ttggaacgtt actaaacaaa
     8941 agttttcaga cggcctttca gggtcgtctg aaaaagtggt ttcagaataa gaatgaagca
     9001 atcggcattt aggccgtctg aaatcaaaag taccgtttcc ccatatcgaa aatccgccat
     9061 gcggtaaaaa tacttccttc aaggagcaaa aatgaatttg ttgaacttat ttaatccgtt
     9121 gcaaactgcc ggcatggaag aagagtttga tgccatcaaa atcggtattg cctctcccga
     9181 aaccatccgc tcatggtctt atggcgaagt caaaaaacct gaaaccatca actaccgtac
     9241 gttcaaacct gagcgtgacg gtttgttctg tgccaaaatc tttggcccgg tcaaagacta
     9301 cgaatgcttg tgcggaaaat acaaacgctt gaaatttaaa ggtgtaactt gtgaaaaatg
     9361 cggcgtggaa gtaaccctgt ccaaagtgcg ccgcgaacgc atgggccata tcgaattggc
     9421 tgcgcccgtc gcacatattt ggttcttaaa atccctgcct tcccgcttgg gtatggtgtt
     9481 agacatgact ttgcgcgata tcgaacgcgt attgtacttt gaagcatttg tggtaaccga
     9541 tcccggtatg actccgctgc aacgccgcca attgctgact gaagacgatt actacaacaa
     9601 gctggacgaa tacggcgacg acttcgatgc caaaatgggt gcagaaggta tccgcgaatt
     9661 gctgcgtacc ttgaatgtag caggcgaaat cgaaatcctg cgccaagagt tggaatcgac
     9721 tggttccgac accaaaatca aaaaaatcgc caaacgcttg aaagtattgg aagccttcca
     9781 tcgttccggt atgaaactgg aatggatgat tatggatgtg ctgccggtat tgccgcctga
     9841 tttgcgtccg ttggttccat tggatggtgg tcgttttgcc acttccgatt tgaacgattt
     9901 gtaccgccgc gttattaacc gtaacaaccg tctgaaacgt ctgttggaac tgcacgcgcc
     9961 tgacatcatc gtccgcaacg aaaaacgtat gttgcaagaa gcagttgact cgctgttgga
    10021 taacggccgt cgcggtaaag ccatgaccgg cgccaacaaa cgcccgctga aatcattggc
    10081 agacatgatt aaaggtaaag gcggtcgctt ccgtcaaaac ctgttgggca aacgtgtgga
    10141 ctactccggc cgttccgtga ttaccgtagg cccgtacctg cgtctgcacc aatgcggttt
    10201 gccgaaaaaa atggctttgg aactgttcaa accgttcatt ttccacaaat tggaaaaaca
    10261 aggtttggcc tctaccgtta aagcagcgaa aaaattggta gagcaagaag taccggaagt
    10321 atgggacatc ttggaagaag tcatccgcga acatccgatt atgctgaacc gtgcgccgac
    10381 cctgcaccgt ttgggtattc aagcgttcga acctatcttg attgaaggta aagcgattca
    10441 gttgcaccca ttggtgtgtg ctgcgttcaa cgccgacttt gacggcgacc aaatggcggt
    10501 acacgttcca ttgagcttgg aagcacaaat ggaagcacgc acgctgatgc tggcttcaaa
    10561 caacgtattg tctcctgcca acggcgaacc gattatcgta ccttcccaag acatcgtatt
    10621 gggtctgtac tacatgaccc gcgaccgtat caatgccaaa ggcgagggca gcctgtttgc
    10681 cgatgtgaaa gaagtgcatc gcgcatacca taccaaacag gttgaactgg gtacgaaaat
    10741 taccgtacgt ctgcgcgaat gggtgaaaaa cgaagcaggt gagtttgagc ctgtcgttaa
    10801 ccgttacgaa acaaccgtcg gccgtgcatt gttgagcgaa atcctgccga aaggcctgcc
    10861 gtttgaatat gtcaacaaag cgttgaagaa aaaagaaatt tctaaactga ttaacgcatc
    10921 gttccgcctg tgcggcttgc gcgatacggt tatctttgct gaccacctga tgtacaccgg
    10981 tttcggattt gcggcaaaag gcggtatttc cattgccgtt gacgatatgg aaattccaaa
    11041 agaaaaagcg gccttgctgg ctgaagccaa tgccgaggtt aaagaaatcg aagaccaata
    11101 ccgtcaaggt ttggttacca acggcgaacg ctacaacaag gtggtcgata tttggggtcg
    11161 tgccggcgat aaaatcgcta aagcgatgat ggacaacctg tccaaacaga aagttatcga
    11221 ccgtgccggc aacgaagtcg atcaagagtc attcaactcc atctatatga tggctgactc
    11281 cggtgcccgt ggttctgcgg ctcagattaa acagttgtcc ggtatgcgcg gtttgatggc
    11341 gaaacctgac ggctcgatta ttgaaacgcc gattacctca aacttccgcg aaggtctgac
    11401 cgtattgcaa tactttattg cgacccacgg tgcgcgtaag ggtttggcgg ataccgcatt
    11461 gaaaaccgcg aactccggtt acctgacccg tcgtctggta gacgtaactc aagatttggt
    11521 cgttgttgaa gacgattgcg gtacttcaga cggctttgtc atgaaggcag tggtacaagg
    11581 cggtgatgtg attgaagcat tgcgcgatcg tattttgggc cgtgttaccg cgtctgacgt
    11641 tgtcgatccg tcaagtggcg aaacattggt tgaagcaggc acgttgttga ctgaaaaact
    11701 ggtggatatg attgaccaat ccggtgtcga tgaagtcaaa gtccgtacgc cgattacttg
    11761 taaaacccgt cacggtttgt gcgcgcactg ttacggccgc gacttggcgc gcggcaaact
    11821 ggtcaatgcc ggcgaggcag tcggcgtgat tgccgctcag tctatcggcg aaccgggtac
    11881 ccagttgacc atgcgcacgt tccacatcgg tggtgcggca tcccgtgcgg cagcagccag
    11941 ccaagtggaa gccaaatcca acggtacggc acgtttcagc agccagatgc gttacgttgc
    12001 caacaacaaa ggtgagttgg ttgtcatcgg tcgctcttgc gaagtcgtga ttcatgatga
    12061 catcggccgt gaacgcgaac gccacaaagt accttacggt gccatcctgc tggtacaaga
    12121 cggtatggcc attaaagccg gtcaaacctt ggcaacctgg gatccgcata cccgtccgat
    12181 gatcaccgaa catgcaggta tggtgaaatt tgagaacgtg gaagagggtg ttaccgttgc
    12241 gaaacaaact gacgatgtaa ccggtttgtc cactttggtg gtgattgacg gtaaacgccg
    12301 ttccagcagc gcgtccaaac tgctgcgtcc gactgtaaaa ttgttggatg aaaacggcgt
    12361 ggaaatctgt attcccggta cgtccactcc ggtttcgatg gcattccccg ttggtgcggt
    12421 gattaccgta cgcgaaggtc aggaaatcgg taaaggcgac gtattggcgc gtatcccgca
    12481 agcctcttcc aaaacccgcg acattaccgg tggtctgccg cgcgttgccg aattgtttga
    12541 agcacgcgtg ccgaaagatg ccggtatgtt ggcggaaatt accggtaccg tttccttcgg
    12601 caaagagacc aaaggcaagc aacgtctgat tgttactgac gtggacggtg tagcatacga
    12661 gaccttgatt tccaaagaga aacaaattct ggtacacgac ggtcaagtgg taaaccgcgg
    12721 tgaaaccatc gtggacggag ctgttgatcc gcatgatatt ctgcgtttgc aaggtatcga
    12781 agcactggca cgctacattg tccaagaggt gcaagaggtt taccgtctgc aaggtgtgaa
    12841 gatttctgat aaacacatcg aagtcatcat ccgtcaaatg ttgcgccgtg tgaacattgc
    12901 ggatgccggc gaaaccgggt tcattaccgg agagcaggtc gaacgcggcg atgtgatggc
    12961 ggccaatgaa aaagctttgg aagaaggcaa agagccggca cgttacgaaa acgtattgct
    13021 gggtattacc aaagcctccc tgtccaccga cagtttcatt tctgccgcat cgttccaaga
    13081 aacgacccgt gttctgaccg aagccgcgat tatgggcaaa caagacgaac tgcgcggttt
    13141 aaaagagaac gtcatcgtcg gccgcctgat tcctgccggt accggtttga cttatcaccg
    13201 cagccgtcat cagcaatggc aaggggtaga gcaggagact gctgaaaccc aagtaacgga
    13261 tgaataatct tcggcgcgtt tattcaatac aaaaccgcaa gctttaagct tgcggttttt
    13321 ctttgtccga ttaaggcaaa aacaagcgtt ttcgtcattt tgaggcgtgt ggattattcc
    13381 ttaggtattt tcgggccgga gaccaacgag gtggcgggtg tcgtcggtac gtccggagac
    13441 caaaataact ttgccaagga tgttggtttc ggcggtcaaa aaagtagcgt cttaatgttt
    13501 tccatttaaa caaatgtcgt ctgaaacttc agacggcatt tcctttaaga aataaatatg
    13561 aaacccagaa atctcttttt tgcaggctgc ctgctgactt cggcgacgtt tgccgaggat
    13621 atcggcgtac ctgtcgaact gattaacgtc ggtaatcgga ttgcgatgcc gtctgaaggg
    13681 gaaagcctcg ccctcctgcc gtttgccgag gatgtaccgc cggttcgcga tgcaatgccg
    13741 tctgaagttc ctaaaagcgc ggcaggcggc gatgttcggg gtgaccggat aggaatgccg
    13801 attaacatcg gatgagcgcg gctttatggc ataaaaaact gtcgtggaaa ggatttacac
    13861 cccaaataaa tttccgttac aacaagatca gcagcaatat gcccgccttt tattcgcgca
    13921 gcggcaagga acggtttgtc agtatagaaa aaacgtattg acagtatttt cttcagtcgt
    13981 ccgactgatt gtgagggatg tcggtaaata tttatcggca aacaagaaaa tcatctttct
    14041 tcttgtcgtt atgcttgact gtatacttgc aataaaaata taattccact cttgccgaca
    14101 tggtgtcggc aagtatttaa ctcaacagga cgagaaaata tgccaactat caaccaattg
    14161 gtacgcaaag gccgtcaaaa gcccgtgtac gtaaacaaag tgcccgcact ggaagcctgc
    14221 ccgcaaaaac gcggcgtgtg cacccgtgta tacacgacta cccctaaaaa acctaactct
    14281 gcattgcgta aagtatgtaa agtccgcctg accaacggtt ttgaagtcat ttcatacatc
    14341 ggcggcgaag gccacaacct gcaagagcac agtgtcgtat tgattcgcgg cggtcgtgta
    14401 aaagacttgc caggtgtgcg ttaccacact gtacgcggtt ctttggatac tgcaggcgtt
    14461 aaagaccgta aacaagcccg ttccaaatac ggtgctaagc gtcctaaata attactggga
    14521 cttaaatagg cacgtcggcc gcctaagctg aacaacggcc gagtaagtga atactcaatt
    14581 gggtattcat gggaatagac ccgactgaat agattaaagg aaattaaaat gccaagacgt
    14641 agagaagtcc ccaagcgcga cgtactgcca gatcctaaat tcggcagcgt cgagttgacc
    14701 aaattcatga acgtattgat gattgacggt aaaaaatccg ttgccgagcg tatcgtttac
    14761 ggtgcgttgg aacagattga gaaaaaaacc ggcaaagtag caatcgaagt atttaacgaa
    14821 gccattgcaa acgccaaacc tatcgtggaa gtgaaaagcc gccgtgtagg tggtgcaaac
    14881 taccaagttc ctgttgaagt tcgtccttca cgccgtttgg ctttggcaat gcgctgggtt
    14941 cgcgatgcgg cccgcaaacg tggtgagaaa tccatggacc tgcgtttggc aggcgaattg
    15001 attgatgcgt ccgaaggccg tggcggtgcg ttgaaaaaac gtgaagaagt acaccgtatg
    15061 gctgaagcca acaaagcatt ctctcacttc cgtttctaat tttgaaaggc taataaaatg
    15121 gctcgtaaga ccccgatcag cctgtaccgt aacatcggta tttccgccca tatcgatgcg
    15181 ggtaaaacca cgacgacaga acgtattttg ttctataccg gtttgaccca caagctgggc
    15241 gaagtgcatg acggtgcggc tactaccgac tacatggaac aagagcaaga gcgcggtatt
    15301 accattacct ccgctgccgt tacttcctac tggtccggta tggcgaaaca attccccgag
    15361 caccgcttca acatcatcga caccccggga cacgttgact ttaccgtaga ggtagagcgt
    15421 tctatgcgtg tattggacgg cgcggtaatg gtttactgcg cggtgggcgg tgttcaaccc
    15481 caatctgaaa ccgtatggcg gcaagccaac aaataccaag tgccgcgctt ggcgtttgtc
    15541 aataaaatgg accgtcaggg tgccaacttc ttccgcgttg tcgagcaaat gaaaacccgt
    15601 ttgcgcgcaa accctgtacc tatcgtcatt ccggttggtg cggaagacaa cttcagcggt
    15661 gtggttgatt tgttgaaaat gaaatccatc atttggaatg aagccgataa aggtacaacc
    15721 tttacctatg gcgatattcc tgccgaattg gtcgaaactg ccgaagaatg gcgtcaaaat
    15781 atgattgaag ccgcagccga agccagcgaa gaactgatgg acaaatactt aggcggcgac
    15841 gagctgaccg aagaagaaat cgtaggcgcg ttgcgtcaac gtactttggc aggcgaaatt
    15901 cagcctatgc tgtgtggttc tgcatttaaa aacaaaggtg ttcaacgtat gttggacgca
    15961 gttgtagaat tgctgccagc tcctactgat attcctccgg ttcaaggtgt caacccgaat
    16021 accgaggaag ccgacagccg tcaagccagc gatgaagaga agttctctgc attggcgttc
    16081 aaaatgttga acgacaaata cgtcggtcag ctgaccttta tccgcgttta ctcaggcgta
    16141 gtaaaatccg gcgataccgt attgaactcc gtaaaaggca ctcgcgaacg tatcggtcgt
    16201 ttggtacaaa tgactgccgc agaccgtact gaaatcgaag aagtacgcgc cggcgacatc
    16261 gcagccgcta ttggtctgaa agacgttact accggtgaaa ccttgtgtgc ggaaagcgcg
    16321 ccgattatct tggaacgcat ggaattcccc gagccggtaa tccatattgc cgttgagccg
    16381 aaaaccaaag ccgaccaaga gaaaatgggt atcgccctga accgcttggc taaagaagac
    16441 ccttctttcc gtgtccgtac agacgaagaa tccggtcaaa ccattatttc cggtatgggt
    16501 gagctgcact tggaaattat tgttgaccgt atgaaacgcg aattcggtgt ggaagcaaat
    16561 atcggtgcac ctcaagtggc ttaccgtgaa actatccgca aagccgttaa agctgaatac
    16621 aaacatgcaa aacaatccgg tggtaaaggt caatacggtc acgttgtgat tgaaatggaa
    16681 cctatggagc cgggtggtga aggttacgag tttatcgatg aaattaaagg tggtgtgatt
    16741 cctcgcgaat tcattccgtc tgtcgataaa ggtatccgcg atacgttgcc taacggtatc
    16801 gttgccggct atcctgtagt tgacgtacgt atccgtctgg tattcggttc ttaccatgat
    16861 gtcgactctt cccaattggc atttgaattg gctgcttctc aagcgtttaa agaaggtatg
    16921 cgtcaagcat ctcccgccct gcttgagccg attatggcag ttgaagtgga aaccccggaa
    16981 gaatacatgg gcgacgtaat gggcgacttg aaccgccgtc gcggtgttgt attgggtatg
    17041 gatgatgacg gtatcggcgg taaaaaagtc cgtgccgaag tacctctggc agaaatgttc
    17101 ggttactcga ccgacctgcg ttctgcaacc caaggccgcg ctacttactc tatggagttc
    17161 aagaaatatt ctgaagctcc tgctcacata gctgctgctg taactgaagc ccgtaaaggc
    17221 taatcaggca aataggccgt ccgaaaggct gaaatgattt ttcagacggc attgttcttt
    17281 aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAWKQJ010000029        16761 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000029 DAWKQJ010000000
VERSION     DAWKQJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.029"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..1304)
                     /locus_tag="WJL20_000717"
     CDS             complement(135..1304)
                     /locus_tag="WJL20_000717"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ7682894.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1478..1588)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1641..2537
                     /locus_tag="WJL20_000718"
     CDS             1641..2537
                     /locus_tag="WJL20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ7682895.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2581..3645)
                     /gene="tsaD"
                     /locus_tag="WJL20_000719"
     CDS             complement(2581..3645)
                     /gene="tsaD"
                     /locus_tag="WJL20_000719"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ7682896.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3761..4948)
                     /gene="ccsB"
                     /locus_tag="WJL20_000720"
     CDS             complement(3761..4948)
                     /gene="ccsB"
                     /locus_tag="WJL20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ7682897.1"
                     /translation="MTEHYKTLPEHELLMQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLCAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4941..6893)
                     /locus_tag="WJL20_000721"
     CDS             complement(4941..6893)
                     /locus_tag="WJL20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ7682898.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7170..7793)
                     /locus_tag="WJL20_000722"
     CDS             complement(7170..7793)
                     /locus_tag="WJL20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ7682899.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7999..8628
                     /locus_tag="WJL20_000723"
     CDS             7999..8628
                     /locus_tag="WJL20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ7682900.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8749..11145)
                     /locus_tag="WJL20_000724"
     CDS             complement(8749..11145)
                     /locus_tag="WJL20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ7682901.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11297..12412
                     /locus_tag="WJL20_000725"
     CDS             11297..12412
                     /locus_tag="WJL20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ7682902.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12415..13014
                     /locus_tag="WJL20_000726"
     CDS             12415..13014
                     /locus_tag="WJL20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ7682903.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13015..13662
                     /gene="pilO"
                     /locus_tag="WJL20_000727"
     CDS             13015..13662
                     /gene="pilO"
                     /locus_tag="WJL20_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ7682904.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELTSIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13680..14225
                     /locus_tag="WJL20_000728"
     CDS             13680..14225
                     /locus_tag="WJL20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ7682905.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14244..16529
                     /locus_tag="WJL20_000729"
     CDS             14244..16529
                     /locus_tag="WJL20_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ7682906.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4495 a         4782 c         3815 g         3669 t
ORIGIN      
        1 attttgggga attttgcaaa ggtctctatc tgtactgtct gcggcttcgt cgccttgtcc
       61 tgatttttgt taatccacta tataaaaatg ccgtctgaac tgttcagacg gcatttttga
      121 ttttcaaacc ggaattacag ccccgctgcc gccctcaatg cagcagcttt gtcggtgcgc
      181 tcccaagtga actcaggttc ttcgcggccg aaatgtccgt aagcggcgga tttactgtaa
      241 atcgggcgca agagatcgag catttggacg atgcctttgg ggcgcaggtc gaaatgttct
      301 cgaactaagg taatcagttt ttcctcgctg attttgcctg tgccgaaagt gtcgatggaa
      361 atcgaagtcg gttcggcaac gccgatggcg taggaaactt ggatttggca ttgggttgcc
      421 aaaccggcgg cgacgatgtt tttcgcaacg tagcggcagg cgtaagcggc ggaacgatcc
      481 actttggacg ggtctttgcc ggagaacgcg ccgccgccgt gcggagccgc gccgccgtag
      541 gtatcaacga tgattttacg gccggtcaaa ccgcagtcgc cttgcgggcc gccgatgacg
      601 aagcggccgg tcgggttgat caggtatttg gtttcgtcgg tcagcagttc agacggcaaa
      661 acgggcttga taatctgttc gatgacggcg tttttcagct cttcataacc gatttccggg
      721 tcatgttgcg tagagaggac gactgtatca atgcgtttta ccttaccggt ttcgctgtcg
      781 taaaccacgg tcagttgggc ttttgcgtcg ggacgcagcc acggcaggcg gccgtcttta
      841 cgcaattcgc tttggcgttg catcaggcgg tggctgtaat agatggcaaa cggcatcagg
      901 gtcggggttt cgtcgcaggc atagccgaac atcaaacctt ggtcgcccgc gccctggttc
      961 aggtcgatgc cttcgccttc gttcacgcct tgggcgatgt cgggggattg ctggtcgtag
     1021 tacacgccga ccgcgcagcc gttggcatca aagcccagct cggacgagtt gtagccgatg
     1081 cgtttgatgg tttcgcgtgc gactttgatg tagtccactt gggcggtggt ggtgatttcg
     1141 cccgccaata cgcacaagcc tgtgttgacc aaggtttctg cagcgacacg cgctttgggg
     1201 tcttgcgcca agatggcatc caaaatcgca tcggacactt ggtcggcaac tttgtccgga
     1261 tggccttcgg ataccgattc ggaagtaaac agatattcgc tcattgctat tctttcatta
     1321 aaaacaggct gctgtgtttt cagacggcct tttatttcgg acagcggaaa tcataacaaa
     1381 aactccgctt tttctctata caagtgagaa acattcgcac ttgtctgctg tttcggaatc
     1441 ctgttgcgcg tttcttacag gcaaaaaaat tcccgcatta agcgggtttg gattgctctg
     1501 gtgagccaca tcggcttttc aaccgtccac cttacttttc cctttgaaaa gcaggttggc
     1561 atggaattcc caactcttaa tgcagcgcgg atggtagcag aaaagccttt ccgccgcaat
     1621 acttcccttt ttcagacggc atgtttcgtt tacaattcgg gctgtttccc cctttgcgaa
     1681 ccgccatgca catcctgttg accgccctgc tcaaatgcct ctccctgctg ccgctttcct
     1741 gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta aaggaagacc
     1801 gcgcgcgcat cgtcgccaat atgcgtcagg caggcatgaa tcccgacccc aaaacggtca
     1861 aagccgtttt tgcggaaacg gcaaaaggcg gtttggaact tgcccccgcg tttttcagaa
     1921 aaccggaaga catagaaaca atgttcaaag cggtacacgg ctgggagcac gtgcagcagg
     1981 ctttggacaa acacgaaggg ctgctgttca tcacgccgca catcggcagc tacgatttgg
     2041 gcggacgcta catcagccaa cggctgccat tccccttaac cgccatgtat aaaccgccga
     2101 aaatcaaagc gatagacaaa atcatgcagg cgggcagggt tcgcggcaaa ggaaaaaccg
     2161 cgcctaccag catacaaggg gtcaaacaaa tcatcaaagc cctgcgttcg ggcgaagcaa
     2221 ccatcgtcct gcccgaccac gtcccctccc ctcaagaagg cggggaaggc gtatgggtgg
     2281 atttcttcgg caaacctgcc tataccatga cgctggcggc aaaactggca cacgtcaaag
     2341 gcgtgaaaac cctgtttttc tgctgcgaac gcctgcctga cgggcagggt ttcgatttgc
     2401 acatccgccc cgtccaaggg gaattgaacg gcgacaaagc ccatgatgcc gccgtgttca
     2461 accgcaatgc cgaatattgg atacgccgtt ttccgacgca gtatctgttt atgtacaacc
     2521 gctacaaaat gccgtaacga aaataaaaat gccgtctgaa caatttcaga cggcattttg
     2581 tcatctgacg atttccgaca gcggccagcg cggacggaca ttgaacgcac cgacctccct
     2641 gcccttgccc aggtgcatcg caccggcaaa ggcaatcatg gcaccgttgt ccgtgcagta
     2701 tgccatcggc gggaaaaaca cgctgacttt ttcggacgga tgtttcggct tgcctttggg
     2761 ggtcgggatt tgcaccgtca tgttgccgaa agtttcgcgg agtttgcggt ttgcaccgac
     2821 cccgccggcg accactacgg ttctgaaccc tgtctgcaac agggcttttt tcactttcgc
     2881 cgccaacaca tctaccaccg catcttgaaa cgcacggcag atgtcgttgc gtgtctgctc
     2941 aggaatgtca tccgccccgt tttccgcgcg cactttctca acggcggtca atacggcggt
     3001 tttcaaacct gaaaaactca tctgcaaatc gtcggaatga atcattgggc gcggaaaaac
     3061 aaacgcttcg ggcctgcccg attccgcaag ttccgacagt ttcgcaccgc ccggatacgg
     3121 caagcccagc agtttcgccg ttttgtcgaa tgcctcgccc gccgcatcat cgacgctctc
     3181 gcccaaaagc gcgtagtcgc ctatacccct gaccgccata atctgcgtat gcccgcccga
     3241 aaccagcagc gcgacaaaag gaaagtcggg tttttcctcc gccaacagcg gcgacagcag
     3301 atgtccttcc aaatgatgga cggggatgac gggcttgccc aatgccaaag ccagtgcatt
     3361 ggcgtagctc gaacccgcca gcagcgcacc gcccaaaccg ggtccctgcg taaaggcaac
     3421 cgcgtcaatg tcgccatacg atgcgcccgc ctgcgccaaa cagccttccg tcaacggaac
     3481 aaggcggcgg atatggtcgc ggcttgccaa ttccggcaca accccgccat attcggcgtg
     3541 cattgccatc tgcgtgtgca gacagtgcgc ccgcaatcct cgttccgtat cgtaaagcgc
     3601 aacgccggtt tcgtcgcaag aagattcgat tcctaatacc aacatggtct gatgccgtta
     3661 aaaactgaaa aacgtattct agcggatttc ggcacgactg ccgtatccca aaaacggaac
     3721 atgccgtctg aagcccttca gacggcatcg tcgcaccgta tcaaagcgtt ccgtaagaat
     3781 gcagcccgct caaaaacata ttcacgccga taaaggcgaa tgcggttacg aacaaaccga
     3841 taatcgccca ccacgccagc actttgccgc gccaaccggc aaccagccgc aagtgcagcc
     3901 aaacggcgta attgagccag acgatgaacg cccacgtctc tttcgggtcc caactccaat
     3961 agcgtcccca agcatccgcc gcccacagcg cacccaaaat ggtggcaatg gtaaagaaca
     4021 gaaagccgac ggcaatcgcc ttatacatga cttcctcaat caacacggat tgcggcagcc
     4081 acgatttccc gccttttcct tccgcacaca gggaaaccag ttcggcaata ccgagcatcg
     4141 cggaaatgca aaacgcgccg taaccgataa agtttgccgg aacgtggatt ttcatccacc
     4201 aagattgcaa tgccggaatc agcggctgga tggtatgcgc ctcgcgggac acgctgtacc
     4261 acaagacaaa tccaaccacg accgccataa agccgaacac gaagccgccc aatttctgta
     4321 tggcgaactt accttcataa taaagataca tcagcgcggt aatcaccaaa aacaggatga
     4381 acacttcata caggttggaa accggaatat gccccgcatc gggacggagc agatagcttt
     4441 cgtgccaacg taccagcaga ccggtaaagc ctgctacggc agacacccat gcaaacacgg
     4501 ttcccatacc caacagcgtg ttggtcggca cattttttac gcttgccaaa accgcgcccg
     4561 aaatataggc gaacagggcg aagaagacaa aggcgcactg ccacatgatc gccgactggc
     4621 tgctgaggaa ataccgcaac aggaaaatct ctgccgattt gatgttgccg ccatacaaac
     4681 cgacggcggc ataggcaagc aatacgctta aaggaacaaa ccagcgcatc ggtttgaaaa
     4741 accaacccaa aaacacggca ataccggcac ttgcccacaa catgaccgtt tcgtaaatgt
     4801 ccatatgcat accggaacgg gtctgtacga aaaccgtagc cgcaaaaacc agcacggcaa
     4861 atacccaatc ccaaagattc agattgctga tcaaagactt ctgcatcagc agctcgtgtt
     4921 ccggaagggt tttatagtgt tcagtcatga ttcaagtcct tgccgagccg ttgcagactc
     4981 tcgacgtgtt ttggaaattc cttctgcaaa tcccgttcgc tgcgggccga agacatggca
     5041 aaacggattt tgccgtctga aaacaatacc cacgcccgtt tttcgcgcac ataaaacatc
     5101 aataccgtac ccaataccaa cagcaccgag ccgagataga ccaaaagcgc acccggggaa
     5161 cgggtcatct gcaaacccga cgaacgcacc tcggaaaacc catcaagttg cagcagcata
     5221 ggcgcgggat attcggtcaa acccgtgtac gcatccatac tgtgcagcag gaaacgattc
     5281 cgcgcttcat cctgctgcca ttcgggcaag ccgtaccggc gtatggtttc atccaaagca
     5341 gcgttcatca cgccgtaaag catttcgtag aaatagccct gcatcttatc ctgctgctct
     5401 ttcgggatat tggacgtaat aaattcgtcc aatcccaaat agcctttttg tgcaaagatg
     5461 ttcagcgtgt tttccgcagc cagcatgaat tgttcgcgga tttcggcagg tgcgcctttg
     5521 gttgcgtcgg caaccagacg tttgcgccct tccccatctt tcaaaaactc acgcaatgcc
     5581 ataaaggtgt ccgctttcaa ctgcttgtcc aaggggatac gcagccagcg gtattgctgc
     5641 tgcaagccgc tgcgcgtgcc ggtaatccaa aaataatcct gttcctgcaa aaccggcagc
     5701 atatagtttt tatattcgac cgcctgccct gccgcatcac ggatacggta aacaatggaa
     5761 gggccgatat tggtgtattt tttaccttcc tgagtaacgg cgcggacatc gttcagcgtg
     5821 gatttcaggc ttttttcccg ttccgcgccc tcgctcatgt cctccacatt catagaagta
     5881 aactgatcga actcaagacg atatttgtgt ttgccaattt ccaacggaaa ctggtgtatg
     5941 gatgttgcct tcaacacgac aggctcgcgc gaagcatcac ccaaattcca cgccttgaat
     6001 gtcaaatccg aaccgccgtc ggcaaaactc gcctgataaa tcgtgatgcc gtgcaaggtc
     6061 aaaggatggt tcacgcggat ggtgcgctcg agtttctcac cggttgcctt atccgttact
     6121 tcaatatcac tggcaaaatc gcgcggcata cccgtattgt aaaaatcgat atggaatttt
     6181 ttcagtttga cttcaaaagg caagtcctga accaatatcc cgttgtcggc attcaggaaa
     6241 accacatccg cactctgccc ctcggaaata ttgacgttgc ccctaaatga gagattggac
     6301 gcactcaaaa tactttcggg cttgaaatcc ttggcataaa ccgcctgatt gtccggaaca
     6361 atccgaccgg tcagcatacc cagtttcaac agcaggttac tgtctatcaa cccgcccagg
     6421 caaatgacaa tcaaagcaac atgggcaaag atatagcccc atttgttcat cgtgcctttt
     6481 ttggcggcaa tcagaaccga cccgccctca cggctgacgg tttttccctg aaaaccctgc
     6541 acctccagat aacgtttggc aacctcgggc gcaattttta catccaacag cgaagaatgg
     6601 cgcatcgccg ccagagattt ttctttaacc ttttcccgaa aagacttcat ttcgcgccag
     6661 aacggcggca cattgcgaat caggcacaaa ctggtagaaa ccaccaaaaa catcatgata
     6721 acgacaaacc atgccgaagc atagacgtca tacagtccca gaaaaccaaa aatctgcgcc
     6781 caaaaaggtc cgaatttgac caaataatcc gtctgcggct ggttttgctg caacaccgta
     6841 ccgataaccg atgcaatacc cagcagactg agcaaagcga ctgcaaagcg catggagctg
     6901 aaaaaagcga accacggacg ggaaagaagt gggggagatc tacgggattt actcattgtg
     6961 tgtttattcc gccatcagga atatgggaaa gcagaattgg gcaaacagaa aacaacgtcc
     7021 cgattctact gtcttgatgc tttttatttc aagacaatga agacagcctg catcgattcc
     7081 aacggttgcg attgaaaaaa cttatcgcag aattgcctga agccgtctga aaacttttca
     7141 gacggcctct aaaacagact attgcggaat taacgcaaac cttggataaa gttggcgacc
     7201 gctttcaaat cttcttcaga catacggttt gcaatatctt ccatgatggt atttttacgc
     7261 tgaccggact tgtaggcatt catctgttca acaatatatg cctgatgctg accgcccaaa
     7321 cgcggataag cctgaatttc gcttccgcct cccggcatac ccgcaccgct cggaccgtgg
     7381 caggacatac acgccggcac ttttttatcg ctcaaaccgc cgcgatagat tttcgcaccc
     7441 aattcgggat tttccttagg attggcttca ccggatttgg gctgctgttt ggcatagaat
     7501 gcggatacgt tcaaaatatc ctgatcgctc aaattcatta ccaccggttt catcacagct
     7561 gccgaaccgt gggtgcgttt accgtcgcgg atgccgatag tttgatgata gatgtaagca
     7621 gtatgctgtg ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc tgctgcatgg
     7681 caagccgcac aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc tttgggagag
     7741 gcggaaaccg caccggcagc caaaacaaag gccaataaag tcaatcgttt catggagtgc
     7801 tcctgattac agcattggat aacgcaacaa tgctcttttt atattcaaat acgggatttt
     7861 tgacccgatt aaaaccgatg attctgtaaa cgtgttattc tatactaaat ttacattaaa
     7921 ttaccactgt gtttcacata aaaccaaccg catatttttg ctgtcggaca aacggcggcg
     7981 gaaaacaagg atatgcccat gaaccttttt caaaacgcca aattcttcac gacggtcaac
     8041 caccttaaag acctgcccga cacccctctc gaaattgcct ttgtcggcag gagcaatgcc
     8101 ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc gtcttgccta cgtttcaaaa
     8161 acacccggac ggacgcagca tatcaacttc ttcgagctgc agaacggcaa ttttatggtc
     8221 gatttgcccg gctacggtta tgcccaagtc cccgaagcag tacgcgcaca ttgggtcaat
     8281 ctgctcggcg actatctgca acagcgcaaa cagcttatcg ggctggtttt gattatggat
     8341 gcccgccatc ctttaaaaga actcgacatc cgtatgctgg actttttcca cacgaccggc
     8401 agaccggttc acatcctgct gtcaaaagcc gacaaattat ccaaaaacga acagataaaa
     8461 accctgtccc aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa catcagcgta
     8521 cagctgtttt ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac tgtcggaagc
     8581 tggtttgacg cagcagatgc cgccgcttcc tctccaaagg aaaactgacc ccaattatac
     8641 ggaaaccgta tttcccccac ttgaccgacc gcaaacattt aaaaaattgc cactgccaaa
     8701 tctaaaatgc cgtctgaaag acttttcaga cggcattttg cggagtcttt aaaacagaga
     8761 atccaactgc tgctgtttgg aaccagtatt actcggaagc accggcgttt cctgcatatc
     8821 ttggcggact tcgtcatccg ccgcctgccg tccgccttct gccgcgcccc cgtcatcttc
     8881 ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg tccagcgtca agcccggatc
     8941 ggttaccata cgttccttca tatagtattc gccattgctg ctgaccacac cttcaggcat
     9001 tttcatcccc ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca cccaaaccgg
     9061 caccgcaatc gtaccgccgt agccgacacg ccccatactc ttaggtttgt cgaagccgat
     9121 atatacggca gtaaccacat cagggttaaa accgacaaac cacgcatcct tattgtcatt
     9181 ggtcgtaccc gttttaccgg caatatccgt tcttcccaac gcagctgccc cccttgccgt
     9241 accaacacgg accacatcct gcataatctt atacataata taggcattgc gcggatcgat
     9301 tgcctgaggc gcattttgcc cggccaccaa aggttgcatt tgggcgcgca acctgccgtc
     9361 tctgtcataa atcttatcga ttacgtgcga agaaacccta tatccgccgt tcgcaaatac
     9421 gctatatgcc tccgccactt tcaacggcgt tgtctcgccc gtacctaaag ccatagacag
     9481 gcttgccggc agctcggacg acctgaagcc gaaacgccgg atatactgtt gcgcgtaacc
     9541 gacaccgata gacatcaaaa tacggatgga aaccatattc ttggaagccg tcagagcctg
     9601 tctcaaagta atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc aaaccgaacc
     9661 gttcggccct ttccccggca gggaaatcgg cgcatcgtta accactgtgg acgcggtcat
     9721 ccccttagat aatgccgccg aatagacaaa cggcttaaag gtcgaacccg gctgccgcat
     9781 tgcctgaacg gcacgattga atgttttgct gtgaaaatca taaccgccga ccagcgcgcg
     9841 cacagctccg gtttttgcat ccagcgaaac caaagccccc tgcagcaacg gctcttgaac
     9901 caccgcccaa cgcccgccgt tgtttttgac acggatgacc gcgcccctgc ggatacggtc
     9961 ctcccccatt ttttcattat tgaccgcgcg ggccgcaaag cccaaggcgc gcctatcaag
    10021 cgtaacccgc ctgccgccgg gcagctgtat gacgacattt ttctttttag tcacatccaa
    10081 cacaacggcg ggaaccattt tatcgacggt atagagtccc gacagatact ggctgacagt
    10141 ctcctcgaca tcttcactct tactcaaatc gatatagttt tccgcaccgc ggtagctgct
    10201 gccgcgatcg aaattccgta gagccttgcg caatgcctcg gttgccacct tctgatgatc
    10261 ggcgcggacc gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac cgtatttctc
    10321 atacagttcc tgacgcacca tttccgccac atataaagca ctctgatcga ttttccgaac
    10381 aaaccgctcg taatgcagtt cctcattcaa cgcctgatcg cgctgttgca cggtaatcat
    10441 cttctcctcg agcatattgt tcaaaatata cttctggcgc aacttggcac gttctggatt
    10501 aacaatcgga ttataggcag acggagcctt gggcagtccc gcaagcatgg cggcttccgc
    10561 caaagtcaaa tctcggacat tcttattgaa atagatttgc gcggcagatg caaaaccata
    10621 ggcgcgctga ccgaggtaaa tctgattgaa atacaactcg aggattttgt ctttgcttaa
    10681 agactgctcg attttatagg caagcaacac ctcattgaat ttgcgtgtga acgttttttc
    10741 actgctcaaa taaaaatttt tcgccacctg ctgcgtaatc gtactcgcac ccgactgcac
    10801 gctgccggac acgacattgc cgacggcagc gcgggcaaca ccccaaacat ccacccccca
    10861 atgccggtaa aagcgtttgt cctcggcggc gataaccgca ttccgcaaca cctctgggaa
    10921 atcgccgatt tttgtaaatt cgcgccgctg ctccccatac ataccgatga cttccccatc
    10981 cgccgaataa atagtcaacg gcattttagg ctggtaatgc tgcaaagaat ccaaagacgg
    11041 cagtttcgga tacgttacca aaatagcaat ggcaaccaaa cccactccaa atacacaaaa
    11101 cccaaaaacc aaaccaaaac aagtcgttaa aatcttttta atcatagctg aataataatt
    11161 taccattatt ggtattaaat aaagtaaaat agcaaccgat ttctacaaag cacggtttca
    11221 atgtgcaaag aacaaggaat ccattacgga taccgaaacg gttactcact gtacaaataa
    11281 agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa aaacagatgc
    11341 caagctccct aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca tcgatatcga
    11401 ccagcattcc atcaaaatgg tccaattgtc aggacgtagt ttaaaccaaa ttcaattgga
    11461 aaaatacgtc attgccaaat taccaaagaa tatcattcaa ggcaataaag tccaaaatta
    11521 cgatcaactt gttacatatt tgcaacaagc ctatgccaaa ctgggtactt cgtgcaaaaa
    11581 catcatcgcg tccgtcccgc aaaatctggc aaccatcgaa caattgacct acacagacaa
    11641 agatgcagaa ttagacctgc aggggttcgt ggagtcctcc atctccgaag tcagctcgat
    11701 atcgctcgaa gaagccaatt acgactatca ggtcttgtcc caatcggccg ccggcgaagc
    11761 tgtgttggcc gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg cattcaacgc
    11821 cgccggtatg aaattatccg cgcttgatgt ggacattttc ggacaataca acgcctacgc
    11881 gctatggata aaccatttcg cccccgagct tgcagccgaa aaagtcgcca ttttcggcgt
    11941 atatgccgca cagacctacg ccttggtcat ccaagacgga aaaatcctat acaaacagga
    12001 aacctccgtc agcgaagaac agctcaacca actcatccag cgcacctatc aggtaacaga
    12061 agaaaaagcg gaagaaatca tcaactcccc gcaaaaacct tccgattacc aagaaagcgt
    12121 ggcaaactat ttcaaccagc agattaccca agaaatacaa agggtcttgc agttttatta
    12181 caccacgcag accgcagacg atatgaccga catcaagcat atcctgctga ccggggaagc
    12241 ggcgcgccag gaaggcatcg cccaaaccgt cgcctcacaa accaatgcag atgtacaatg
    12301 cgtccatccc gcgcgttatt ttgcggacaa cctcaaaaca gacaaacaac aattcgaact
    12361 tgatgcgccg acactgacca gggcgttcgg tttggcggta cggggattat aattatgaac
    12421 aatttaatca aaatcaacct cctcccctac agggaagaga tgaacaagcg caaacagcag
    12481 cagtttaaaa cgctgatgta cggtgccgtg ctgacgggcg ttgccgccgt tgcggcaacc
    12541 tacctgttta tcgacaatat gatcaataac cagtcggaaa gaaacacgct gctggaaacc
    12601 tccatcgcac acttggatac cgagctgtcg gaaatacaaa agctcaaaca ggaaaaagat
    12661 gccttcctga ttaagaaaaa caaaatcgag gagctccagc tcaaacgcct ccaagccgca
    12721 aaaatcctcg acagcctgaa tgaggccgtc cccggaagca cctacctgac ctcgctggat
    12781 gccgttaccg ccgactctta tcggctcagc ggcaggacat ccagcgacaa ccgcgttgcc
    12841 gccatgatga gggcgatgcc caataccggc atattcaagc aacccgaatt gttaagcatc
    12901 aagaaaaaca attcgcatca agaatttacc cttcaggcaa cattacaacc catcgtaaag
    12961 gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa ctgaatggct
    13021 tctaaatcat ctaaaaccaa cttggatctc aacaaccttc acctgctcaa ccttcctgcc
    13081 aggcttttta tcgccctgct ggccgttgcc gccgtgctgg ggctcggtta tgccggattg
    13141 ttcaaaagcc agatggaatc ccttgaggaa tacgaagcaa aagaaaccga actgaaaaac
    13201 acctacaaac agaaaagtat cgacgcggcc agcctgaaca acctgaggga cgaacttacc
    13261 tcaatccgct ctgccttcga tatcatgttg aaacagctgc cgacagatgc agaaattccc
    13321 aacctggttc aagagcttca tcaggcaggt tcgagcaacg gcctgcgctt ggacagcgtt
    13381 atgccccaac ctcccgtaga tgacggcccc atcaaaagat taccctattc catttccatt
    13441 accggaaatt acgaacagat cagccaattt acccgcgatg tcggcagcct ctcccgaatc
    13501 attacccttg agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa tcctgacggc
    13561 aagagcagca tcctgaacct cagcgccatt gccaccacct accaagcaaa atccgtagaa
    13621 gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt agggaaacca
    13681 tgaaacacta tgccttactc atcagctttt tggctctctc cgcgtgttcc caaggttctg
    13741 aggacctaaa cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa atcatacctt
    13801 tccaagcacc taccctgccg gttgcgccgg tatacagccc gccgcagctt acagggccga
    13861 acgcattcga cttccgccgc atggaaaccg acaaaaaagg ggaaaatgcc cccgacacca
    13921 agcgtattaa agaaacgctg gaaaaattca gtttggaaaa tatgcgttat gtcggcattt
    13981 tgaagtccgg acagaaagtc tccggcttca tcgaggctga aggttatgtc tacactgtcg
    14041 gtgtcggcaa ctatttggga caaaactacg gtagaatcga aagcattacc gacgacagca
    14101 tcgtcctgaa cgagctgata gaagacagca cgggcaactg ggtttcccgt aaagcagaac
    14161 tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc agcacctgcc gcagaacaaa
    14221 attaagaaga ggattactcc attatgaata ccaaactgac aaaaatcatt tctggtctct
    14281 ttgtcgcaac cgccgccttt cagacagcat cggcaggaaa cattacagac atcaaagttt
    14341 cctccctgcc caacaaacag aaaatcgtca aagtcagctt tgacaaagag attgtcaacc
    14401 cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa caaaccggca
    14461 tttccatgga tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa atcagtgccg
    14521 cacaaaacag cagccgtgcg cgtctggttc tgaatctgaa caaaccgggc caatacaata
    14581 ccgaagtacg cgggaacaaa gtttggatat tcattaacga atcggacgat accgtgtccg
    14641 cccccgcacg ccccgccgta aaagccgcgc ctgccgcacc ggcaaaacaa caggctgccg
    14701 caccgtctac caagtccgca gtatccgtat ccgaaccctt taccccggca aaacaacagg
    14761 ctgccgcacc gtttaccgag tccgtagtat ccgtatccgc accgttcagc ccggcaaaac
    14821 aacaggcggc ggcatcagca aaacaacagg cggcagcacc agcaaaacaa caggcggcgg
    14881 caccggcaaa acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc ggcattatcg
    14941 aattggcggc attgggcttt gccgggcagc ccgacatcag ccaacagcac gaccacatca
    15001 tcgttacgct gaaaaaccat accctgccga ccacgctcca acgcagtttg gatgtggcag
    15061 actttaaaac accggttcaa aaggttacgc tgaaacgcct caataacgac acccagctga
    15121 ttatcacaac agccggcaac tgggaactcg tcaacaaatc cgccgcgccc ggatacttta
    15181 ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac aatgcgccca
    15241 aaaccttcac aggccggaaa atctcccttg acttccaaga tgtcgaaatc cgcaccatcc
    15301 tgcagatttt ggcaaaagaa tccgggatga acattgttgc cagcgactcc gtcaacggca
    15361 aaatgaccct ctccctcaaa gacgtacctt gggatcaggc tttggatttg gttatgcagg
    15421 cacgcaacct cgatatgcgc caacaaggga acatcgtcaa catcgcgccc cgcgacgagc
    15481 tgcttgccaa agacaaagcc ttcttacagg cggaaaaaga cattgccgat ctgggcgcgc
    15541 tgtattcaca aaacttccaa ttgaaataca aaaatgtgga agaattccgc agcatcctgc
    15601 gtttggacaa tgccgacaca accggaaacc gcaatacgct tgtcagcggc aggggcagcg
    15661 tgctgatcga tcccgccacc aataccctga ttgttaccga tacccgcagc gtcatcgaaa
    15721 aattccgcaa actgattgac gaattggacg tacccgcgca acaagtgatg attgaggcgc
    15781 gtatcgtcga agcggcagac ggcttctcgc gcgatttggg cgttaaattc ggcgcgacag
    15841 gcaagaaaaa gctgaaaaat gatacaagcg cattcggctg gggggtaaac tccggcttcg
    15901 gcggcgacga taaatggggg gccgaaacca aaatcaacct gccgattacc gctgccgcaa
    15961 acagcatttc gctggtgcgc gcgatttcct ccggtgcctt gaatttggaa ttgtccgcat
    16021 ccgaatcgct ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc caaaaccgca
    16081 aagaggccaa aatcgaatcc ggttacgaaa ttcctttcac cgtaacctca atcgcgaacg
    16141 gcggcagcag cacgaacacg gaactcaaaa aagccgtctt ggggctgacc gttacgccga
    16201 acatcacgcc cgacggccaa atcattatga ccgtcaaaat caacaaggac tcgcctgcgc
    16261 aatgtgcctc cggtaatcag acgatcctgt gtatttcgac caaaaacctg aatacgcagg
    16321 ctatggttga aaacggcggc acattgattg tcggcggtat ttatgaagaa gacaacggca
    16381 atacgctgac caaagtcccc ctgttgggcg acatccccgt tatcggcaac ctctttaaaa
    16441 cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt cattaccccg aggattatgg
    16501 gtacggccgg caacagcctg cgctattgat gcgtcaaaat aagggcatat gttttacggc
    16561 atatgccctt tctttatgct ttttgccgcg accgaaatgc cgtcattccc gcgcaggcgg
    16621 gaatccaact tgtccggttc cggttgtttt tcgtctcgta acttttgagc cgtcattccc
    16681 gcgcaggcag aaatccagtc cgttcagttt cggtcatttc cgataaattc ctgttgcttt
    16741 tcatttctgg attcccactt t
//
LOCUS       DAWKQJ010000030        16750 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000030 DAWKQJ010000000
VERSION     DAWKQJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.030"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WJL20_000730"
     CDS             complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WJL20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ7682907.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAVVEGAVEPAEPSLHA
                     VSEEMPVQEVLLDELPSEEPVKSVQAKTAADILAERMKNLPHEPRHTAGSASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1912..2046
                     /gene="rpmH"
                     /locus_tag="WJL20_000731"
     CDS             1912..2046
                     /gene="rpmH"
                     /locus_tag="WJL20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ7682908.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2049..2414
                     /locus_tag="WJL20_000732"
     CDS             2049..2414
                     /locus_tag="WJL20_000732"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ7682909.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2479..2700
                     /gene="yidD"
                     /locus_tag="WJL20_000733"
     CDS             2479..2700
                     /gene="yidD"
                     /locus_tag="WJL20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ7682910.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2873..4510
                     /gene="yidC"
                     /locus_tag="WJL20_000734"
     CDS             2873..4510
                     /gene="yidC"
                     /locus_tag="WJL20_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ7682911.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDNLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4591..5304)
                     /locus_tag="WJL20_000735"
     CDS             complement(4591..5304)
                     /locus_tag="WJL20_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ7682912.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHDQRAVVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(5363..5953)
                     /gene="maf"
                     /locus_tag="WJL20_000736"
     CDS             complement(5363..5953)
                     /gene="maf"
                     /locus_tag="WJL20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ7682913.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6101..6604
                     /locus_tag="WJL20_000737"
     CDS             6101..6604
                     /locus_tag="WJL20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ7682914.1"
                     /translation="MSDPNLIDPEVFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6638..6817
                     /gene="rpmF"
                     /locus_tag="WJL20_000738"
     CDS             6638..6817
                     /gene="rpmF"
                     /locus_tag="WJL20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ7682915.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6992..7519
                     /locus_tag="WJL20_000739"
     CDS             6992..7519
                     /locus_tag="WJL20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ7682916.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7607..8662
                     /gene="plsX"
                     /locus_tag="WJL20_000740"
     CDS             7607..8662
                     /gene="plsX"
                     /locus_tag="WJL20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ7682917.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAVNQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            8798..9056
                     /locus_tag="WJL20_000741"
                     /pseudo
     CDS             8798..9056
                     /locus_tag="WJL20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
     gene            9083..9499
                     /locus_tag="WJL20_000742"
     CDS             9083..9499
                     /locus_tag="WJL20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682918.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTVYFEKGYYLPMIQTILEDGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHTSKNISTIQNIISEFMIQKQTPAPII"
     gene            9519..9722
                     /locus_tag="WJL20_000743"
     CDS             9519..9722
                     /locus_tag="WJL20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682919.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            9749..10711
                     /locus_tag="WJL20_000744"
     CDS             9749..10711
                     /locus_tag="WJL20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ7682920.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10758..11129
                     /locus_tag="WJL20_000745"
     CDS             10758..11129
                     /locus_tag="WJL20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ7682921.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            11155..12081
                     /gene="fabD"
                     /locus_tag="WJL20_000746"
     CDS             11155..12081
                     /gene="fabD"
                     /locus_tag="WJL20_000746"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ7682922.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDGQVKAICAESAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQIAAFIEAH"
     gene            12220..14085
                     /gene="msbA"
                     /locus_tag="WJL20_000747"
     CDS             12220..14085
                     /gene="msbA"
                     /locus_tag="WJL20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ7682923.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            14185..15750
                     /gene="guaA"
                     /locus_tag="WJL20_000748"
     CDS             14185..15750
                     /gene="guaA"
                     /locus_tag="WJL20_000748"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ7682924.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            15812..16558
                     /gene="fabG"
                     /locus_tag="WJL20_000749"
     CDS             15812..16558
                     /gene="fabG"
                     /locus_tag="WJL20_000749"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ7682925.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4236 a         5128 c         3953 g         3433 t
ORIGIN      
        1 ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg tctgaaaacg
       61 ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc aattccggat
      121 cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgcc
      181 caccaaacga atcgccgata gacggcaggc tcaaagtggt cagttctttg gtcaggctca
      241 tggcaacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
      301 ttttgatgcg gtaatatttc gccaccgcat cgatgatgat gtcggcggtg atgactttgt
      361 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
      421 ggttcataaa gcggctgctc gctccgacac ggttaaacgc gccttcaagc tcgcgcacgt
      481 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcaatactg atgcccgccg
      541 cttccgcctt tttctgcaaa atggcgatac gcatttccaa ttcgggcggc tcgagttcca
      601 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc cataccttcg attttggcgg
      661 gtaaaacatc gcaagtgagg atgagctgtt ttttctcgtt gtggaaatgg ttgtacagat
      721 agaagaattc ctccatcgta cggtcttttc ctttaataaa ctgaatatcg tcgataatca
      781 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta ggtattgttg cgaaccgcct
      841 tcataaaact gcggatgtag tcgtccgaat gcatatagcg cactttggca tcgggacggt
      901 ttttcaacag ttcgttgccg accgcctgca caaggtgggt tttacccaaa cccgtactgc
      961 catagaggaa gaacgggtta taactctgtc ccggactttc cgcaatcgcc tgcgcggcag
     1021 ctgccgcaag gcggttgccc ttgccttcta ccaacgtatc aaatgtgtaa tccggagaca
     1081 ggttggtttg ttcgtaacgc gcctcttccg catcacgctg cgcatccgtc cgcgctttcg
     1141 caagtacctc cgattccgga cgggaagcag acccggcagt atggcgcggc tcatgcggga
     1201 ggtttttcat acgttccgcc aaaatatccg ccgccgtttt cgcctgtacg gatttgacag
     1261 gctcttcaga cggcagctcg tccaacagaa cctcctgcac gggcatttcc tccgacaccg
     1321 catgcaagga cggctcggca ggttcgacag caccttcaac caccgccatc tcataacgca
     1381 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     1441 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg ttcttgccgt
     1501 acaccaccca tacgccaccc tcctcaccga cggtaagggg cgcaatccat tgcgcaaact
     1561 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctagtg
     1621 tcatagataa gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     1681 aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc cgattatacc
     1741 gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa ggcggcagaa
     1801 atccgcaggc aatcgggcaa tcctcctgca aagtttctat attgattgac aaaagcggca
     1861 aattggagtg taattcacgg tttatctatc taccgatttt taggaaacat catgaaacgc
     1921 acttatcaac cttccgttac caaacgcaaa cgcacccacg gcttcctcgt gcgctccaaa
     1981 acacgcggcg gccgcgcagt attggcggcg cggcgcgcca aaggccgcaa acgcctggcg
     2041 gtataatttt ggactaccgc ttcggaaggc agtaccgctt gttgaaaacg gatgattttt
     2101 catccgtttt cgcattcaga aaccgccgca gccgcgacct gctgcaagtt tcgcgttcaa
     2161 acggcaatgg gctggatcat ccccgcatcg gtctggtggt cggcaaaaaa accgccaaac
     2221 gcgccaacga acgaaattat atgaagcgcg ttatccgcga ctggttcaga ttgaacaaaa
     2281 accggctgcc gccgcaggat ttcgtcgtgc gcgtccgccg taaattcgac agggctaccg
     2341 caaaacaggc aagggcggaa ctggcacaac tcatgttcgg caaccccgca accggatgca
     2401 ggaaacaggc atgatcagaa cggtactctg caggcaaggt tcagacggca acgggtttcc
     2461 catacaagga acatcccgat gaacttccta ttgtccaaac tcctgctggg actgatacgg
     2521 ttctaccaat attgcatcag cccgctgatt ccgccgcgct gccgctatac gccgacctgt
     2581 tcgcaatacg cggtcgaagc ggtcaaaaaa tacggcgcat tcaaaggcgg ccggctcgcc
     2641 atcaagcgca ttgcacgctg ccaccctttc ggcgggcacg gacacgaccc cgttccctga
     2701 cccgacgcaa tattcaaatt gcacgctttc cttttatttc ccatcggttt ctatataatg
     2761 ccgtctgaac cttcgggctg gcggcacgac cgtcgggtat gaagcccgcc cttattcccc
     2821 gtctatcgga acacgcaacc tgcggcattt ccgaccattc aggaaactct atatggattt
     2881 taaaagactc acggcgtttt tcgccatcgc gctggtgatt atgatcggct gggaaaaaat
     2941 gttccccacc ccgaaacccg tccccgcacc ccaacagacg gcacaaaaac aggcagcaac
     3001 cgcttccgcc gaagccgcgc tcgcgcccgc aacgccgatt accgtaacga ccgacacggt
     3061 tcaagccgtc attgatgaaa aaagcggcga cctgcgccgg ctgaccctgc tcaaatacaa
     3121 agcaaccggc gacgaaacca aaccgttcgt cctgtttggc gacggcaaag aatacaccta
     3181 cgtcgcccaa tccgaacttt tggacgcgca aggcaacaac gttctgaaag gcatcggctt
     3241 taccgcaccg caaaaacaat acagcctcaa cggcgacaaa gtcgaagtcc gcctgagcgc
     3301 gcccgaaacc aacggactga aaatcgacaa agtctatacc ttcaccaaag gcagctatct
     3361 ggtcaacgtc cgcttcgaca tcgccaacgg cagcggtcaa accgccaacc tgagcgcgga
     3421 ctaccgcatc gtccgcgacc acagcgaacc cgagggtcaa ggctacttta cccactctta
     3481 cgtcggccct gttgtttata cccctgaagg caacttccaa aaagtcagct tctccgactt
     3541 ggacgacgat gccaaatccg gcaaatccga ggccgaatac atccgcaaaa ccccgaccgg
     3601 ctggctcggc atgattgaac accactttat gtccgcttgg attttgcagc ctaaaggcgg
     3661 acaaagcgtt tgcgccgctg gcgactgccg tattgacatc aaacgccgca acgacaacct
     3721 gtacagcacc agcgtcagcg tgcctttagc cgctatccaa aacggcgcga aatccgaagc
     3781 ctccatcaac ctctacgccg gcccgcagac cacatccgtc atcgcaaaca tcgccgacaa
     3841 cctgcaactg gcaaaagact acggcaaagt acactggttc gcttccccac tcttctggct
     3901 cctgaaccaa ctgcacaaca tcatcggcaa ctggggctgg gcaatcgtcg ttttgaccat
     3961 catcgtcaaa gccgtactgt atccattgac caacgcctct taccgctcta tggcaaaaat
     4021 gcgtgccgcc gcacccaaac tgcaagccat caaagagaaa tacggcgacg accgtatggc
     4081 gcaacaacag gcgatgatgc agctttacac agacgagaaa atcaacccgc tgggcggctg
     4141 cctgcctatg ctgttgcaaa tccccgtctt catcggattg tattgggcat tgttcgcctc
     4201 cgtagaattg cgccaggcac cttggctggg ttggattacc gacctcagcc gcgccgaccc
     4261 ctactacatc ctgcccatca ttatggcggc aacgatgttc gcccaaactt atctgaaccc
     4321 gccgccgacc gacccgatgc aggcgaaaat gatgaaaatc atgccgttgg ttttctccgt
     4381 catgttcttc ttcttccctg ccggtctggt attgtactgg gtagtcaaca acctcctgac
     4441 catcgcccag caatggcaca tcaaccgcag catcgaaaaa caacgcgccc aaggcgaagt
     4501 cgtttcctaa atgccgcagc atgaaaaatg ccgtctgaaa cctgttcaga cggcattttt
     4561 attgctcacc ccctatcggg gcggaaatct tcaacctgca tacatcacaa aaatcgtcgg
     4621 gcgttttttc agattgggca tttcttttct ttttcgccac tgcacaaccg tctggctgat
     4681 gatttcctga gtcggcaacg tcaaatccgt agccgtgcac aagcgcgttt caggatgcag
     4741 gttttccacc gcatccgcca acaacacatc gttgcgatac ggcgtttcga taaacagctg
     4801 cgtttcattc tgctggcgcg aacgttgctc caaagccttc aaactctgaa tccgctcgtt
     4861 tttttcagac ggcagataac ccttaaacgc aaaactctgc ccgttcgcac ccgaagccat
     4921 caaagccagc agcaggctgg aaggcccgac cagcggacgc acttcaaaac cgtgtttatg
     4981 cgccaatgcc accaaattcg cacccggatc agccacagcc gggcaacccg cctcgctgac
     5041 aatgcccata ctgcgccctt cttgcaaagg tttcagcaat tccggcaaag tcttcaaatc
     5101 cgtgtgttcg ttcagcgttt gcaaattcag ttcgcggata ggcattgtca cgcccaaatg
     5161 tttcaaatgc gcgcgcgccg ttttttccgc ttccacgaca aaatccgtca gcccgacaac
     5221 cgcccgttga tcatgcggca acaggcacgg cgtatcgggc gtacccaaag gcgtaggaat
     5281 caaatacaaa acaggagaca ccattccctc actcatcagt taaaaatgcc gtctgagcct
     5341 ttcagacggc ataaacgggc agttacagaa cctccacgcc ctcatttttc aagaaatcga
     5401 ccagacggaa aaccggcaaa ccgattaaag cattcggatc gctgctttca atccgttcga
     5461 tcagcagcgc gcccaagccc tcgctcttca acgcacacga gcaataaacc gcatcaggct
     5521 cgcgctccaa atagcggagg atgtgcagct cgtccaactt cctcatcacg accaccgtct
     5581 tatcgatatg ccgctgcatc ctgcccgtaa ccgtatttag cagaacgacc gcgctgtaaa
     5641 actcaatctc cctgccgctc aaatgcatca acatcttttg cgcattagca aggttcatcg
     5701 gcttgcccca ctgcctgccg tcgcaccacg ccacctggtc cgcaccgaca atcaacgcct
     5761 ctgggaaacg cccggtcaac gaccgcgcct taccctcggc aaggcgcaat gccgtctgag
     5821 gggcggattc ccccaacatc ggcgtttcgt caaaatcggg ggacgccgcc tgaaaggcaa
     5881 tgccgagcct ttccatctgt tcgcggcgga aaaccgaact cgtacccaaa atcaaaggca
     5941 gttccaaacc catcccatcc tccttaccgt tgaaaacacg cccgaagggg cggtaaaatc
     6001 cagccatgcg ccgaaacacg ggtatccgcc ttcggcgtac cgcaacattt ttcttaaaaa
     6061 tattgacgtt agaacatcta aattatatca tatctcgttt atgtcagacc ctaatttgat
     6121 tgacccagaa gttttcgccg ccgaagggca gaacctgcaa ggcagttttc tgctggaaga
     6181 attggatgaa cgcgtcagtt cgcacgatta tcccgccgac aggcagacca aaatatcgtt
     6241 tacactgacc ggcggtcgcg accggctgca acgcctgttc ctcgacctga acgtcaaagc
     6301 cgatatgccc ctgatttgcc agagatgtat caaacccatg ccgttcatgc ttgatgaaag
     6361 cagccgtatc gtcctgtttt ccaacgaaga gtccttggac gaatccatgc ttgccgacga
     6421 agaactcgaa ggcatactga ttgaaaaaga actcgacgtg cgcacattgg tagaagacca
     6481 aatcctgatg tccctgccct tttcgccgcg acacgaagac tgcggcgaca atgggacact
     6541 ggaagaagtc aatcgggaca aacccaaccc ctttgctgtt ttggcaggtt tgaaaagcaa
     6601 ttgattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac aaaacaaaaa
     6661 atccccttcc aaacgcggta tgcaccgttc gcacgacgcg ctgaccgcgc ccgcactgtc
     6721 tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca acggtatgta
     6781 ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact gactgaaaaa
     6841 gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca tccgtccaaa
     6901 ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataataaccc gtgaaaatat
     6961 tccaaaagcc ccaaccacca aggaaattcc gatgaaacag aaaatctggt acacctacga
     7021 tgacatccac cgcgtcatca aagcattggc agaaaaaatc cggaacgccg acatcaaata
     7081 cgatgccatg attgccatcg gcggcggcgg ctttattccg gcacgtatgc tgcgctgttt
     7141 tctggaaatt ccgatttatg ccgtaaccac cgcctattac gacagcgaca acgaaggaca
     7201 ggttacagaa gaagtcaaaa aagtccaatg gctcgacccc gttcccgaag ccctgcgggg
     7261 caaaaacgtc cttgttgtcg atgaagtgga cgacagccgc gtaaccatgg aattttgcct
     7321 gacagagctg cttaaagaag acttcggcac catcggcgtc gccgtactgc acgaaaaaat
     7381 caaagccaaa gcaggcaaaa tccccgaagg cattccctat ttcagcggca tcaccgtaga
     7441 agactggtgg atcaactatc catgggacgc actcgacatc gacgaacaca accgccttgc
     7501 cgaggccggc cgaggctgac cctttcagac ggcatatttt ccgaaccgat gccgtctgaa
     7561 gcccgcacga cccctgccgc agaccgaaaa cctaccggag aaccctatga ttacacttgc
     7621 cgtagatgcc atgggcggcg accaaggact tgccgttacc gtacccggcg caaccgcatt
     7681 cctccaagca caccccgatg tccgcctgat tatgaccggc gacgaaacgc aactgcgcca
     7741 agccctgacc gcggcaggcg caccgatgga acgcatcgac atctgccaca ccgcacaaat
     7801 cgtcggcatg gacgaagcgc cgcaatccgc cctgaaaaac aaaaaagact cctccatgcg
     7861 cgttgccgtc aaccaagtta aagaaggcaa agcccaagcc gccgtatccg caggcaacac
     7921 gggtgcgctc atggcaaccg cacgtttcgt cctcaaaacc attcccggca tcgaacgccc
     7981 cgccatcgcc aaattccttc cttccgacac cgaccatgtt acccttgcac tcgaccttgg
     8041 cgcgaacgtc gactgcacgc ccgaacagct cgcccaattt gccgttatcg gcagcgaact
     8101 cgtccacgca ctccatcctc aaaaaggaca gccgcgcgtc gggctggtca acgtcggcac
     8161 ggaagacatc aaaggtacgg acaccgtcaa acaaacctac aaactgctgc aaaacagcaa
     8221 actcaacttt atcggcaaca tcgaaagcaa cggcatcctc tacggcgaag cagatgtcgt
     8281 cgtcgccgac ggctttgtcg gcaacgtcat gctcaaaacc atcgaaggcg cggtcaaatt
     8341 catgagcgga gccatccgcc gcgaattcca aagcaacctg ttcaacaaac ttgccgccgt
     8401 tgccgcccta cccgccctca aagggctgaa aaacaaactc gatccgcgca aattcaacgg
     8461 ggccatcctg ctcgggctgc gcggcatcgt gattaaaagc cacggcggca cagacgaaac
     8521 cggtttccgc tacgcactcg aagaagccta ccacgaagcc aagtcagccg gcctttccaa
     8581 aatcgaacag ggcgtagccg aacaactcgc cgcactcgaa actgccaaag ccgtccaaaa
     8641 cgaaaatgtc ggcggtctgt aacacgcgcg atgccgtccg aacgcccccg cccctttcag
     8701 acggcatccg cccgccccga acctgcgggc gcggacggcg atgcacctgt ccggcacttc
     8761 ccaaatactt ccctgtaaaa taaggagtat ttgaaaaatg aagacattag aaaaacggat
     8821 gaaagctcta gacaaacaga taatgaagtt cggaaaatcc cttgaaggca ggcttgaggc
     8881 ttgatgcccg tctgattgaa tccgcattgg attatattca ttattcggaa cgttttttgg
     8941 cttttgaaat cctgtgtact tatatcgaag atttcgatgt ccggctgacg gaacaagaat
     9001 cccgggaaat ttcttttatc aacaaggaat ttgagataga aagcacgtcc gattaaccaa
     9061 taaagccaat gggttgataa acatgaaaac atcaacggtt gtttttggcg gattttttat
     9121 tacagataat ggagaacgaa tccaaatccc cgttttggaa aatcctggca ttaaagaaat
     9181 caatcacttt ttttccatat caaattttga gaaaaaagcc ggcgtccttg ttttcagaat
     9241 catccccgag ccggaatttg gcgataccga attaactgtc tattttgaaa aaggatatta
     9301 tttgccaatg attcaaacaa ttttagaaga cggggatatt gaagtcaaaa accttaaaac
     9361 ggaaaattac agtgggaagg caagggaaat tttgggagat tcctacccta ttgagcatac
     9421 ctctaaaaat atttctacaa ttcaaaatat aatttctgaa tttatgatac aaaaacaaac
     9481 accggcacca ataatttgat ggaaacccaa caatcaaaat gccgtctgaa gcagcttggg
     9541 ctttcagacg gcatttcctc cgcttaaaac agcatatcgg caaccccgcc ccgcccgtcc
     9601 acggcaatct gcatctgaaa accatctgta tcccaaacca cacccccatc ccgatttccg
     9661 tcatttgcac cctgcccgta ttgggcaatc atcggttttt cgcttacaat agccgaatct
     9721 gaaccaacta tccaaaaagg ccgttcccat gcagtatgcc aaaatttccg gcacgggcag
     9781 ctatcttccc gccaaccgcg tcagcaatga cgaccttgcc caaaaagtgg atacctcgga
     9841 cgagtggatt accgcgcgca cgggcatcaa attccgccat attgcagccg aaaatgagaa
     9901 aaccagcgat cttgccgccg aagcagcccg ccgcgcactg gatgcagccg gattagacag
     9961 cggcgaaatc gatttaatta ttgtggcgac ggcaacgccg gatatgcagt ttccttctac
    10021 cgccaccatc gtccaacaaa aactgggcat caccaacggc tgccccgcgt ttgacgtaca
    10081 ggcggtgtgt gcgggcttta tgtacgcgct gaccacggca aacgcctaca tcaaaagcgg
    10141 catggcaaaa aacgcgctgg tcatcggcgc ggaaactttc agccgcatcg tcgattggaa
    10201 cgaccgcaca acctgcgtat tgttcggcga cggcgcgggc gcggtggttt tgagcgcggc
    10261 ggacaaaccg ggcatcatcc acagcaaact caaagccgac ggcaattatc tgaaactctt
    10321 aaacgtcccc gggcaaatcg cctgcggcaa agtttccggt tcgccgtaca tttcgatgga
    10381 cggtcccggc gtgttcaagt ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat
    10441 cgaagaagcc ggttataccg ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg
    10501 ccgcattatc gaatcgaccg cgaaacattt aggtttgagt atggacaaag tcgtcctgac
    10561 cgtccaagac cacggcaaca catccgccgc atcgattccg ctggctttgg atacgggcat
    10621 ccgcagcgga caaatcaaac gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt
    10681 cgcgtggggc gcggtgctgt tgcaatattg aacccgatgc cgtctgaaac aggctttcag
    10741 acggcatttc ccatatcatg aagcggcagg ctttcttcaa accaattgcg tgtgcggcat
    10801 ttctgtctgc cgtttcgctg cgcctccccg tattgggcgc gtgttacgca atattgtccc
    10861 tctatgcgtt tgcactttac ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca
    10921 ttcccgaaca ccgcctgctg ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg
    10981 gcagcaggat attcaggcat aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga
    11041 ctgtttcggg caatgtcctg gcgacctgca tcctgattga ttatttcgtt ccgcccgaac
    11101 tttttgtcaa actcgggcaa catctctgac aatccaacca aacaaaggaa taccatgtct
    11161 tttgcattct tctttcccgg acaaggttct caaagcctcg gcatgatgaa cggctttaca
    11221 gggcaggccg tcgtcaaaaa cacatttgac gaagcctccg ccatattggg gcaggacttg
    11281 tgggcgatga ttaacggcag cgatgccgaa atcatcggtc aaaccgtcaa tacccaaccc
    11341 atcatgctcg ccgccggcgt tgccgtttac cgcgcctatt tggaagcggg tggcaaaacg
    11401 cctgccgccg ttgccggaca cagcctcggc gaatataccg cgcttgtcgc cgccggcgca
    11461 ttggattttg ccaacgcggt caaactcgtg cgcctacgcg cagaactgat gcagtccgcc
    11521 gtaccgcaag gcgtgggcgc aatggcggcg attctcggct tggaagatgg gcaggttaaa
    11581 gccatttgtg ccgaatccgc acagggcgaa gtggtcgaag ccgtcaactt caactcgccc
    11641 gggcaaatcg tgattgcagg caacgccgcc gccgtcggac gcgccatggc tgccgccaaa
    11701 gaagccggag ccaaacgcgc cctgccgctg cccgtgtccg taccttccca ttgcagcctc
    11761 atgaaacccg ccgccgacaa acttgccgaa gccttgaaaa ccgttgaaat caagcagccg
    11821 caaatccgcg ttatccacaa cgccgacgtt gccgcctacg atgatgccga caaaatcaaa
    11881 gacgcgctcg tccgccagct ttacagcccc gtacgctgga cggaaaccgt caacgccctc
    11941 gtttcagacg gcattgccga atccgccgaa tgcggcccgg gcaaagttct ggcaggtctg
    12001 gcaaaacgca tcaacaaagc cgccgcgtgc agcgcactga ccgatgccgg acagattgcc
    12061 gcctttatag aagcacactg acttcgttct gtaaaaagca gcctgcatgc ctcaggctgc
    12121 ttttcacgtc cgaacggcaa acagccgcat atttgcgcta taattgatga cgaccaaacc
    12181 accgacagcg gctgccgttg cagttcccgc cccaccgaca tgatagaaaa actgactttc
    12241 ggactgttta aaaaagaaga cgcgcgcagc tttatgcgcc tgatggcgta cgtccgcccc
    12301 tacaaaatcc gcatcgttgc cgccctgatt gccattttcg gcgttgccgc caccgaaagc
    12361 taccttgccg ccttcatcgc ccccctgatt aaccacggct tttccgcacc tgccgcgccg
    12421 cccgagctgt ctgccgccgc cggcatcatt tccaccctgc aaaactggcg cgaacagttt
    12481 acctatatgg tttgggggac ggaaaacaaa atctggaccg tcccgctctt tctcatcatt
    12541 ctcgtcgtca tccgtggcat ctgccgcttt accagcacct atctgatgac ttgggtctcc
    12601 gtgatgacca tcagcaaaat ccgcaaagat atgtttgcca aaatgctgac cctttcctcc
    12661 cgctaccatc aggaaacgcc gtccggcacc gtactgatga atatgctcaa cctgaccgaa
    12721 cagtcggtca gtaacgccag cgacatcttc accgtcctca cgcgcgacac gatgatcgtt
    12781 accggcctga ccatcgtcct gctttacctc aactggcagc tcagcctcat cgttgtcctg
    12841 atgttccccc tgctctccct gctctcgcgc tactaccgcg accgtctgaa gcacgtcatt
    12901 tccgactcgc aaaaaagcat aggcacgatg aacaacgtga ttgccgaaac ccatcaggga
    12961 caccgcgtcg tcaagctgtt caacgggcag gcgcaggcgg caaaccggtt tgacgcggtc
    13021 aaccgcacca tcgtccgcct cagcaaaaaa atcacgcagg caacggcggc acattccccg
    13081 ttcagcgaac tgattgcctc gattgccctc gccgttgtca tcttcatcgc cctgtggcaa
    13141 agccaaaacg gctacaccac catcggcgaa tttatggcat tcatcgtcgc gatgctgcaa
    13201 atgtacgcac ccatcaaaag ccttgccaac atcagcatcc ctatgcagac gatgttcctc
    13261 gccgccgacg gcgtatgtgc atttctcgac accccgcccg aacaggacaa aggcacgctc
    13321 gcgccgcagc gtgtcgaagg gcgtatcagc ttccgcaacg tcgatgtcga ataccgttcg
    13381 gacggcatca aagccctcga caacttcaac ctcgacatca gacaaggcga acgcgtcgcc
    13441 ctggtcggac gttccggcag cggcaaatcc accgtcgtca acctgctgcc ccgctttgtc
    13501 gaaccgtctg ccggcaacat ctgcatagac ggtatcgaca tcgccgacat caaactcgac
    13561 tgcctgcgcg cccaattcgc cctcgtctcc caagacgtat tcctgtttga cgacaccctg
    13621 tttgaaaacg tccgatacag ccgtcccgac gcaggcgaag ccgaagtcct gtccgccctc
    13681 caagccgcca acctgcaaag cctgattgac agctccccgc tcggactgca ccagcccatc
    13741 ggatcgaacg gcagcaactt gtccggcgga cagcggcaac gcgtcgccat cgcccgcgcc
    13801 attttgaaag acgcgccgat attattattg gacgaagcca ccagcgcatt agacaacgaa
    13861 tccgaacgcc tcgtccaaca agcgctcgaa cgcctgatgg aaaaccgcac cggcatcatc
    13921 gtcgcccacc gcctgaccac catcgaaggg gccgaccgca tcatcgtaat ggacgacggc
    13981 aaaatcatcg aacaaggcac acacgaacaa ctgatgtccc aaaacggcta ctacacgatg
    14041 ttacgcaata tctcaaacaa agatgccgcc gtccggacgg cataaacaaa atgccgtccg
    14101 aaatggtata atcgccccga ccctttcaga cggcactata tccgccgacc catccgatta
    14161 tcttcaatca ctgtaaaacc cattatgacc caagacaaaa tcctcatcct cgacttcggt
    14221 tcgcaagtta cccagctgat tgcccgccgc gtgcgcgaag cccacgttta ctgcgaactg
    14281 cattccttcg atatgccttt ggacgaaatc aaagccttca accccaaagg catcatcctt
    14341 tccggcggcc ctaattccgt ttacgaatcc gactatcagg ccgataccgg tatttttgat
    14401 ttgggcattc cggttttggg catctgctac ggaatgcagt ttatggcgca ccacttgggc
    14461 ggcgaagtgc agcccggcaa ccagcgcgaa ttcggttacg cgcaagtcaa aaccatcgac
    14521 agcgaactga cccgcgatat tttcgacgac gcgcccaaca cactcgacgt atggatgagc
    14581 cacggcgaca aagtgtccaa actgcccgac ggcttcgccg tcatcggcaa caccccgtcc
    14641 tgcccgattg ccatgatgga aaacgccgaa aaacaattct acggcatcca gttccacccc
    14701 gaagttaccc acaccaaaca aggccgcgcc ctgttgaacc gctttgtctt ggatatttgc
    14761 ggcgcgcaac cgggctggac gatgccgaac tacatcgaag aagccgttgc caaaatccgc
    14821 gaacaggtcg gcagcgacga agtgatttta ggtttgtcag gcggcgtaga ctcttccgta
    14881 gccgccgcgc tgattcaccg cgccatcggc gaccaactga cctgcgtgtt cgtcgatcac
    14941 ggtttgttgc gcctgaacga aggcaaaatg gtgatggata tgttcgcccg caacttgggt
    15001 gtgaaagtga ttcacgtcga tgccgaaggg cagtttatgg cgaaactcgc cggcgttacc
    15061 gaccctgaga aaaaacgcaa aatcatcggc gcggaattta tcgaagtatt tgatgccaaa
    15121 gagaaaaaac tcaccaacgc caaatggctg gcgcaaggca cgatttaccc cgacgtaatc
    15181 gaatccgcag gcgcgaaaac ccaaaaagcc cacgccatca aatcgcacca caacgtcggc
    15241 ggtctgcccg aaaatatgaa gctcaagctg cttgagccgt tgcgcgactt gtttaaagac
    15301 gaagtgcgcg aactgggcgt tgctttgggt ctgccgcgcg aaatggtgta ccgccatccg
    15361 ttccccggcc ccggtttggg cgtgcgcatc ttgggcgaag tgaaaaaaga atacgccgac
    15421 ttattgcgtc aagcagacga tattttcatc caagaattac gcaacaccac cgacgaaaac
    15481 ggcacatctt ggtacgacct gaccagccag gcatttgccg tattcctgcc tgtgaaatcc
    15541 gtcggcgtaa tgggcgacgg acgcacttac gactacgtcg tcgcactgcg cgcagtcatc
    15601 accagcgact tcatgaccgc acactgggca gaactgcctt actcactgct cggacgcgtg
    15661 tccaaccgca tcatcaacga agtcaaaggc atcaaccgcg tggtgtacga tgtcagcggc
    15721 aaaccgcctg ccaccatcga gtgggaataa ccagcaaaca gggctgcacc gtccggcgca
    15781 gtctttcgat tatcggaaaa aaggaaaaaa tatgagcaca caagatttaa gcggcaaaat
    15841 cgctttggta accggcgcat cgcgcggtat cggtgcagca attgccgaca cactggcggc
    15901 agccggtgcc aaagtcatcg gtacggcgac cagtgagagc ggtgcggcgg cgattagcga
    15961 gcggttggcg caatggggcg gcgaaggccg cgtattaaat tccgccgaac ccgaaaccgt
    16021 cgaaaacctg attgccgaca tcgaaaaaac gttcggcaaa ctcgatattc tggtcaacaa
    16081 cgccggcatc acccgcgaca acctcctgat gcgcatgaaa gaagaagagt gggacgacat
    16141 catgcaggtc aacctcaaat ccgtgttccg cgcttctaaa gccgtcttgc gcggcatgat
    16201 gaagcagcgc gccggccgta tcatcaacat cacatccgtc gtcggcgtga tgggcaatgc
    16261 cggtcaaacc aactatgccg cggcaaaagc aggcttaatc ggtttctcca aatccatggc
    16321 gcgcgaagtc ggcagccggg gcattaccgt caactgcgtc gcccccggct ttatcgatac
    16381 cgatatgacc cgcgccctgc cggaagaaac ccgccaagcc tttaccgccc aaaccgcctt
    16441 gggcagattc ggcgacgcgc aagacattgc cgatgcggtt ttgttcctcg cttccgatca
    16501 ggcgaaatac attaccggcc agacgctgca cgtcaacggc ggcatgctga tgccctgacg
    16561 gacagccgtg ccgacaatgc cgtctgaagc cctttcagac ggcatttgca ttcccaaaca
    16621 aaatgaacac acaccacacc ctgccctgcc cctgcggttc aggcacaagc tgagaccttt
    16681 gcaaaactcc tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc
    16741 cccaaatatc
//
LOCUS       DAWKQJ010000031        16732 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000031 DAWKQJ010000000
VERSION     DAWKQJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.031"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            208..1272
                     /locus_tag="WJL20_000750"
     CDS             208..1272
                     /locus_tag="WJL20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ7682926.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1413..1736
                     /locus_tag="WJL20_000751"
     CDS             1413..1736
                     /locus_tag="WJL20_000751"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ7682927.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1808..2131
                     /locus_tag="WJL20_000752"
     CDS             1808..2131
                     /locus_tag="WJL20_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ7682928.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2178..2381
                     /gene="tatA"
                     /locus_tag="WJL20_000753"
     CDS             2178..2381
                     /gene="tatA"
                     /locus_tag="WJL20_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ7682929.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2385..3071
                     /gene="tatB"
                     /locus_tag="WJL20_000754"
     CDS             2385..3071
                     /gene="tatB"
                     /locus_tag="WJL20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ7682930.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3085..3855
                     /gene="tatC"
                     /locus_tag="WJL20_000755"
     CDS             3085..3855
                     /gene="tatC"
                     /locus_tag="WJL20_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ7682931.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWMVIGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3908..4513
                     /gene="maf"
                     /locus_tag="WJL20_000756"
     CDS             3908..4513
                     /gene="maf"
                     /locus_tag="WJL20_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ7682932.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALPLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4529..4882
                     /locus_tag="WJL20_000757"
     CDS             4529..4882
                     /locus_tag="WJL20_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ7682933.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4872..6686
                     /locus_tag="WJL20_000758"
     CDS             4872..6686
                     /locus_tag="WJL20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ7682934.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWTCRSLVAHFGQERIVTLLAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6876..7553
                     /locus_tag="WJL20_000759"
     CDS             6876..7553
                     /locus_tag="WJL20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7682935.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7566..8972
                     /locus_tag="WJL20_000760"
     CDS             7566..8972
                     /locus_tag="WJL20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ7682936.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9090..11516
                     /locus_tag="WJL20_000761"
     CDS             9090..11516
                     /locus_tag="WJL20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ7682937.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGSADLIIAVTPPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11561..11806
                     /gene="rpsP"
                     /locus_tag="WJL20_000762"
     CDS             11561..11806
                     /gene="rpsP"
                     /locus_tag="WJL20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ7682938.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11822..12331
                     /gene="rimM"
                     /locus_tag="WJL20_000763"
     CDS             11822..12331
                     /gene="rimM"
                     /locus_tag="WJL20_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ7682939.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12331..13080
                     /gene="trmD"
                     /locus_tag="WJL20_000764"
     CDS             12331..13080
                     /gene="trmD"
                     /locus_tag="WJL20_000764"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ7682940.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13096..13461
                     /gene="rplS"
                     /locus_tag="WJL20_000765"
     CDS             13096..13461
                     /gene="rplS"
                     /locus_tag="WJL20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ7682941.1"
                     /translation="MNLIQQLEQEEIARLNKDIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13780..14505
                     /locus_tag="WJL20_000766"
     CDS             13780..14505
                     /locus_tag="WJL20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ7682942.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14541..15416
                     /locus_tag="WJL20_000767"
     CDS             14541..15416
                     /locus_tag="WJL20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ7682943.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15493..16410
                     /locus_tag="WJL20_000768"
     CDS             15493..16410
                     /locus_tag="WJL20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ7682944.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHDHDHGEYDPHVWNDPVLMSAYAQNVAEALIKAD
                     PEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFI
                     APQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLY
                     SDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4365 a         5342 c         3671 g         3354 t
ORIGIN      
        1 cgtctgaagc cgattcggtt ttcagacggc atcattattt gacctgacgc aaacagaaag
       61 ccttccgcgt gcttaaacac atctcttgta gtaaaatatc taaacagatt caactggaaa
      121 cggtcaggct tcttcacgcc attcccattt caaccgccgc ctgccagatt cagacggtat
      181 tcctacttga aaacaaggag cgttacaatg aaagcagcac gtttttacga taaaggcgat
      241 atccgcatcg aagacatccc cgaaccgacc gtcgcccccg gcactgtcgg catcaatgtc
      301 gcctggtgcg gcatctgcgg tactgacctg cacgaattca tggaaggccc gattttcatt
      361 ccgccttgcg gtcatccgca cccgatttcc ggcgagtccg cacccgtaac gatgggacac
      421 gagttctccg gcgtggtcta tgccgtcggc gaaggcgtgg acgacatcaa agtcggccaa
      481 cacgtcgtgg tcgaacccta catcatccgc gatgacgtac cgaccggaga aggcagcaac
      541 taccacctct ccaaagatat gaactttatc ggcttgggcg gctgcggcgg cggtctgtcc
      601 gaaaaaatcg ccgtcaaacg tcgttgggtg catccgattt ccgacaaaat cccgttggat
      661 caagccgctt tgatcgaacc gctgtctgtc ggacaccacg cctatgtacg cagcggcgcg
      721 aaagcaggcg acgtcgcatt ggtcggcggc gcaggtccga tcggtttgct gctggccgcc
      781 gtgttgaaag ccaaaggcat caaagtcatc atcaccgagt tgagtaaagc acgcaaagac
      841 aaagcgcgcg aatccggcgt tgccgactac atcctcgacc cgtccgaagt cgatgttgtc
      901 gaagaagtga aaaaactgac caacggcgaa ggcgtggacg tggcattcga atgtaccagc
      961 gtgaacaaag tgttggatac tttggtcgaa gcctgcaaac ctgccgccaa cttggttatc
     1021 gtatccatct ggagccaccc tgcaaccgtc aacgtccaca gcgtcgtgat gaaagaactg
     1081 gatgtgcgcg gcacgattgc ctactgcaac gaccacgcgg aaaccatcaa actggttgaa
     1141 gaaggcaaaa tcaacctcga gcctttcatc acccagcgca tcaagctgga cgaactggtt
     1201 tccgaaggct tcgagcgtct gatccacaac aacgaatccg ccgttaaaat tattgtgagt
     1261 ccaaacctgt aagcaaatta catttgcaac agcacaaatg ccgtctgaaa cgctcagacg
     1321 gcattttcca acgaagcgca acaggcgtat tgcccgagag cagccgttta gcctatcatt
     1381 ccgaacaaac agaaacaaac agaaggaaaa tcatgggaga ttccgtacta tccgccatcc
     1441 aacaaaccat cacccagcga aaatctgcca atccgtccga atcttacgtc gcacagctct
     1501 tgcataaggg cgaagacaaa atcctaaaaa aagtgattga agaggcgggc gaagtgttga
     1561 tggcatccaa agacaaaaac ccgtcccacc tggtttacga agttgccgac ttatggtttc
     1621 acaccatgat tcttctgaca caccacgacc tgaaggcgga agacgtattg gacgaacttg
     1681 cgcgccgcca aggtttgtcg ggcttggccg aaaaagccgc tcgcacagaa tcttgaattt
     1741 atattaaaat ccgcactttc ccacattcaa tccgtctgac cgctgttcag acggcaacgg
     1801 agccgttatg gacaactgta ttttctgcaa aatcgccgcc aaagacattc cggcgcaaac
     1861 cgtctacgaa gacggcgaaa tggtttgttt caaagacatc aaccccgccg ctccggttca
     1921 tctgctgctg attcccaaag tccatttcga ttcgttggca cacgccgcgc ccgaacatca
     1981 gccccttttg ggaaaaatga tgctgaaagt tcccgaaatc gccaaagcgg caggactggc
     2041 agacggcttc aaaaccctga tcaacaccgg aaaaggcggc ggacaagagg tcttccacct
     2101 gcatatacac atcatgggca cacccgtata aaccgttatt tcacaatcaa cccctaatac
     2161 ttacttaagg atacatcatg ggcagttttt ctctgacgca ctggattatc gtactgatta
     2221 tcgtcgtttt gatattcggc accaaaaaac tgcgcaacgt cggcaaagac ctcggcggtg
     2281 cggttcatga cttcaaacag gggctgaacg aaggtacaga cggcaaagaa gcccaaaaag
     2341 acgatgtaat cgaacacaaa aaagacgaag acaaagcgta atttatgttt gatttcggtt
     2401 tgggcgagct ggtttttgtc ggcattatcg ccctgattgt cctcggcccc gaacgcctgc
     2461 ccgaggccgc ccgcaccgcc ggacggctca tcggcaggct gcaacgcttt gtcggcagcg
     2521 tcaaacagga atttgacact caaatcgaac tggaagaact gaggaaggcc aagcaggaat
     2581 tcgaagctgc cgccgctcag gttcgagaca gcctcaaaga aatcggtacg gatatggagg
     2641 gtaatctgca cgacatttcc aatggtctga agccttggga aaaactgccc gaacagcgca
     2701 cgcctgccga tttcggtgtc gatgaaaacg gcaatcccct tcccgatgcg gcaaacaccc
     2761 tatcagacgg catttccgac gttatgccgt ccgaacgttc ctacgcttcc gccgaaaccc
     2821 ttggggacga caggcaaacc ggcagtacag ccgaacccgc ggaaaccgac aaagaccgcg
     2881 catggcggga atacctgact gcttctgccg ccgcacccgt cgtacagacc gtcgaagtca
     2941 gctatatcga taccgctgtt gaaacccctg ttccgcatac cacttcgctg cgtaaacagg
     3001 caataagccg caaacgcgat ttgcgtccca aatcccgcgc caaacctaaa ttgcgcgtcc
     3061 gtaaatcata aagagagcaa tccggtgtcc gaaacacaaa acgaacaacc cgtccaaccg
     3121 cttgtcgagc atctcatcga gctgcgccgc cgcctgatgt ggatggttat cggcattctc
     3181 gtctgctttt tcggcatgat gccgtttgcc caacaactct atacttttat cgccaacccg
     3241 ctgatggcaa acctgcccaa agacaccagc atgattgcca ccgatgtcat cgcaccgttt
     3301 ttcgtgccgg tcaaagttac cctgatggcg gcatttttaa tttcgctgcc gcatacgctc
     3361 taccaaatct gggcatttgt cgcccccgca ctctaccaaa acgaaaaacg cctgattacg
     3421 ccgctcgtcc tctccagcgt cagcctgttt ttcatcggta tggcatttgc ctactttttg
     3481 gttttccccg tcattttcaa attccttgcc ggcgttaccc ctgtcggggt caatatggcg
     3541 acagacatcg acaaatacct ctccttcatc ttggggatgt ttgtcgcatt cggtacaacg
     3601 tttgaagtcc ccattgtcgt tatcctgtta accaaaattg gtgtggtaac aaccggacag
     3661 ctcaaacgcg cccgccccta tgtgattgtc ggcgcgtttg tcattgccgc catcatcacg
     3721 ccgcccgatg tgatttcaca aaccctgctt gccattccgc tgattctctt atacgaagca
     3781 ggtatttggt tcggacgctt tttcacgcca cgttcagaac aggatggcga catacagccg
     3841 cctgcaacaa cctgacacta tgccgtccga acctccgcct cataccgcca cagattaagg
     3901 aatacctttg aataccctct atttaggttc aaacagcccg cgccgaatgg aaatcctgac
     3961 gcagttgggc taccgcgtca tccaactgcc cgccggcatc gacgaatccg ttaaagccgg
     4021 cgaaacacct ttcgcttacg ttcaaaggat ggcagaagaa aaaaaccgaa ccgccctgcc
     4081 cctcttttgc gaaaccaacg gcacaatgcc cgattttccc ctgattaccg ccgacacctg
     4141 cgtcgtttca gacggcatca tattaggcaa accccgctcc caagccgaag caatcgaatt
     4201 tttaaaccga ttgtccggca aacaacatac cgtcctgact gctgtctgca ttcattatcg
     4261 cggcaaaacg tcaagccgcg tccaaaccaa ccgcgtcgtt ttcaagcccc tgagttcgga
     4321 agaaatttcc gcctatgtgc aaagcggcga accgatggac aaagccggcg cctacgccgt
     4381 acaaggcata ggcggcatct ttatccaatc tatcgaaggc agcttcagcg gcattatggg
     4441 gctgcccgtt tatgaaaccg tttcgatgtt gcaggatttg ggataccgta ccccaccttt
     4501 catccgagcc taacccgtaa agaccgccgt gaacagacaa accgcttacc tccttacctc
     4561 tttcagcctg atcgcactga taatcctgtc cctttcctgg gaactgtgga ttgcaccgtt
     4621 gcgcccgggc ggctcgtggc tcgcgctcaa agccctcccc ctctgtctgc cgctttcagg
     4681 catcttgaaa aagaaaatct atacctacca atacagctcc atgctggttc tgatttactt
     4741 tgccgaagcc gtcatgcgcc tgttcgacgc ctatcccgca gaacagattt gcgccgcgct
     4801 ttccgcagta ttcagcatca tcttcttcat atcctgcctg tcgttcgtca aacaatacaa
     4861 ggaaacaaac aatgcccgct gaaacgaccg tatccggcgc gcaccccgcc gccaaactgc
     4921 cgatttacat cctgccctgc ttcctttgga taggcatcgt cccctttacc ttcgcgctca
     4981 ggcagcaacc gtcgcccgac ttttaccacg atgctgcagc cgcagccggc ctgattgtcc
     5041 tgttgttcct cacggcagga aaaaaactgt ttgatgtcaa aatccccgcc atcagcttcc
     5101 ttctgtttgc aatggcggcg ttttggtatc ttcaggcacg cctgatgaac ctgatttacc
     5161 ccggtatgaa cgacatcgtc tcttggattt tcatcttgct cgccgtcagc gcgtggacct
     5221 gccggagctt ggtcgcacac ttcggacaag aacgcatcgt taccctgctt gcctggtcgc
     5281 tgcttatcgg ctccctgctt caatcctgca tcgtcgtcat ccagtttgcc ggctgggaag
     5341 acacccccat gcttcaaaac attattgttt acagcgggca aggcgtaatc ggacacatcg
     5401 gacagcgcaa caacctcgga cactacctga tgtggggcat actcgccgcc gcctacctca
     5461 acggacaacg aaaaatcccg cccgccctcg gcgcaatctg cctgattatg cagaccgccg
     5521 ttttaggttt ggtcaattcg cgcaccatct tgacctacat agccgccatc gccctcatcc
     5581 ttcccttctg gtatttccgt tcggacaaat ccaacaggcg gacgatgctc ggcatagccg
     5641 cagccgtatt ccttaccgcg ctgttccaat tttccatgaa caccattctg gaaaccttta
     5701 ccggcatccg ctacgaaact gccgtcgaac gcgttgccaa cggcggtttc acagacttgc
     5761 cgcgccaaat cgaatggaat aaagcccttg ccgccttcca gtccgccccg atattcgggc
     5821 acggctggaa cagttttgcc caacaaacct tcctcatcaa tgccgaacag cacaacatac
     5881 acgacaacct cctcagcaac ttgttcaccc attcccacaa catcgtcctc caactccttg
     5941 cagagatggg aatcagcggc acgcttctgg ttgccgcaac cctgctgacg ggcattgccg
     6001 ggctgcttaa acgccccctg acccccgcat cgcttttcct aatctgcacg cttgccgtca
     6061 gtatgtgcca cagtatgctc gaatatcctt tatggtatgt ctatttcctc atccccttcg
     6121 gactgatgct cttcctgtcc cccgcagagg cttcagacgg catcgccttc aaaaaagccg
     6181 ccaatctcgg catactgacc gcctccgccg ccatattcgc aggattgctg cacttggact
     6241 ggacatacac ccggctggtt aacgcctttt cccccgccac tgacgacagt gccaaaaccc
     6301 tcaaccggaa aatcaacgag ttgcgctata tttccgcaaa cagtccgatg ctgtcctttt
     6361 atgccgactt ctccctcgta aacttcgccc taccggaata ccccgaaacc cagacttggg
     6421 cggaagaagc aaccctcaaa tcactaaaat accgccccca ctccgccacc taccgcatcg
     6481 ccctctacct gatgcggcaa ggcaaagttg cagaagcaaa acaatggatg cgggcgacac
     6541 agtcctatta cccctacctg atgccccgat acgccgacga aatccgcaaa ctgcccgtat
     6601 gggcgccgct gctacccgaa ctgctcaaag actgcaaagc cttcgccgcc gcgcccggtc
     6661 atccggaagc aaaaccctgc aaatgacccg cgccggcgga tgcggatacc gcccgaaatg
     6721 taaagctcca tgcaagacat tgcaaaaaac agcaaaccgg tagggaaata cgctatcaaa
     6781 aaacattgca gccgtgttaa gataaaccgt caaacaatct tttcacgccc cgcccgaaac
     6841 agggtcgggg cataccctta cgaaaaggaa acaccatgag ccgcgtatta ctcgtagatg
     6901 acgatgccct gctaaccgaa ctgctgaccg aatacctgag cgccgaaggt ctgaacgtcc
     6961 gcagcgttcc cgacggggaa gcaggcgtac aggaaatcct gagcgggcag tacgatgtag
     7021 tcgtattgga ttccatgatg cccaaaatga acggcttgga tgtcttgaaa aacgtacgcg
     7081 cccgaagcac cgtccccatc atcatgctga ccgccaaagg cgacgacatc gaccgaatca
     7141 tcggcttgga aatgggcgcg gacgactatg tccccaaacc ctgcacacca cgcgaactct
     7201 tggcacgcat caatgccatc ctgcgccgcg cacaacacag cggcgaacag aacaacgcac
     7261 ccaacagcat ctccgtcagc gatgtcgtcc tgtaccccgc caaacgccag gcatccgtca
     7321 aagacatgcc gctcgaactg accagcaccg aattcaacct gctcgaagtc ctgatgcgcc
     7381 atgccggaca ggtagtcagc aaagaaaccc tgtccgtcga agcactcgac cgcaagctgg
     7441 caaaattcga ccgcagtatc gacgtacaca tctccagcat ccgccacaag ttgggcgatg
     7501 cctctctgat tcaaaccgta cgcggcttgg gctacctgtt tgtcaaaaac tgaagcaacg
     7561 ggcaaatgaa actgttccaa cgcatcttcg ccacattttg cgcggttatc gtctgtgcaa
     7621 tctttgtggc gagtttttct ttttggctgg tgcagaacac ccttgccgaa aaccagttca
     7681 accaacgccg caccatcgaa accactttga tgggcagcat catttccgca ttccgggcac
     7741 gcggggacgc gggcgcgcgc gaaatcctga cggaatggaa agacagcccc gtctcatcgg
     7801 gcgtgtacgt tatacagggc gacgagaaaa aagatatcct gaaccggtat atcgacagct
     7861 ataccatcga acgcgcccgg cttttcgccg ccggacaccc gcattccaac ctcgtccata
     7921 tcgaatacga ccgcttcggc gaagaatacc tgttcttcac caaagactgg gacaaactcc
     7981 aagcccgccg cctgcccagc cccctgttga tccccggcct gccgctcgcc ccgatttggc
     8041 acgaactcat catattgtcc ttcatcatca tcgtcggact gctgatggca tatatcctcg
     8101 ccggcaacat tgccaaaccc atcagaatct taggcaacgg catggacagg gtggcaaacg
     8161 gagaacttga aacccgtatc tcccaacagg tcgacgaccg cgacgacgaa ttgtcccatc
     8221 ttgccatcca attcgacaaa atggtggaaa aactcgaaaa actcgttgcc aaagaacgcc
     8281 acctgctcca tcacgtctcc catgaaatgc gttctcccct tgcgcgcatg caggcaattg
     8341 tcggactgat tcaggcgcag ccccaaaaac aggagcaata tctcaaacgg ctggaaggcg
     8401 aactgacccg catggatacg ctggccgggg aactgttaac cctgtcccgt ctcgaaactt
     8461 ccaatatggc tttggaaaaa gaaagcctga aactcctgcc cttcctgggc aacctggtag
     8521 aagacaatca aagcattgcc cagaaaaacg gacaaacggt taccctgtct gccgacggaa
     8581 aaatccccga aaatacaacc atccttgcca acgaaagcta cctgtaccgc gccttcgaca
     8641 acgtcatccg caacgccgtc aactacagtc ccgaaggcag caccatcctg atcaacatcg
     8701 gacaagacca caaacactgg ataatcgacg ttaccgacaa cggccccggc gtggacgaaa
     8761 tgcagctccc gcacatcttc accgctttct accgtgcaga ctccagtgcg aacaaacccg
     8821 gaacaggact ggggcttgca ttgacccaac atattattga acagcactgc ggaaaaatca
     8881 tcgccgaaaa catcaaaccg aacggtctgc ggatgcgctt tatcctgcct aagaaaaaaa
     8941 ccggttccaa aacagaaaaa agtgcgaact gaccataatg ccgtctgaac cggcttcaga
     9001 cggcattgca caaacagtta ccccaaaaca aacacacagc gggtgcatct ccaacaatca
     9061 cgcacatacc gtccgcaaag gaatatatca tgtcggcaca aaccgatccg ggctactttt
     9121 tcatgcccaa ccacattatc ctgataggcg cgagcgaacg tccgtacagc ctgggcgaac
     9181 gcgtactcag caacctgctg agtacgccct ttcaaggaaa aatcaccccc gtaaacccgc
     9241 gccaccacac catagccggg cttcccgcct acaccagcct caacaaaatc cccggcagtg
     9301 cagacctgat tattgccgtt accccgcccg acagttacga caccctcttc aaaacctgcc
     9361 gcaaaaagca gctccgacac atcatcctca tacaggactg ggacagcctg tctgccgcag
     9421 aactgcacac cgccgaaact gccatccgca aacaccacgg caacggactc aacatcaccg
     9481 cctgcaccac cgcaggcatc caactgccct cactcggact taacatcagt acccaagacg
     9541 gatacgccgc aggccatacc gccatactga ccggcaatgc cgccgtcagc cgccaaatcg
     9601 acaacatcct gaacaaactc cgtcaaggca catcccgcca catcagcctg catcccggca
     9661 tcagccccat cacatccgcc gattggctca accgcttcgg acacagcctg cacaccaaaa
     9721 ccgccgtcct acaccacaac cctgaagagg atcagcgcaa actgttcagc gcaatccgcc
     9781 aatttacccg ccatacgccg ctgattctcc acatcacctg ccgcacgaca gaaaccgacc
     9841 gtgccgtact gcactgcctc gcccgacact gcaacttcct catcagtttc aacgccgacg
     9901 acctcgaagc cgcactgcgc gcccaactgt ccgaccttcc acccctgtcc cgactcgaca
     9961 tcctgtccga cacgcctgcc gaatggctga acgcgcacgc gccaaaaacc ctcgccctcc
    10021 actttcccaa ccttccccac cacatccgca acggacacct gaccggcaca cccacaccct
    10081 caatctgcca cgacatcgcc tcacgtcagc ttgcccgccc cgacacccaa gccgtcctga
    10141 ccatcctcag cccctccgga cacgaggatt acaaaaaaac agcacgcgcc cttatccgcc
    10201 tgtccgaaca gaccgccaaa cccctgctcg tcagcagccc cttttcagac ggcataacac
    10261 atttcgacac ccccactcag gcaatccgca ccctttccta ccgcaacacc gccgccgccc
    10321 tgaaacaggc acagctcgac attgcaccgc cgcagccatg ccgtctgaaa acaccgcaac
    10381 cccaaaacat caaaaaagcc cttgcagcgg caaatccctc cctgctcgcc gaagccctgc
    10441 acctcccccc ctaccggcac actacccata acgccgtcca attccaattc ggcagccacc
    10501 ccctctatgg cgacatcctg accgcatgct gcaacggaca aaccactgcc gtactcccgc
    10561 cgtttaccac gctcgacagc cgccaccttg cccgctttgc cgaactcgac ggcacacaaa
    10621 ccctcgacca gttcctgcac acactgaccg tcattcccga ataccgccaa cacattctcg
    10681 gcatcaccct caacctcaac ggcggacaat acagcagcga tttcatctta aagacacccg
    10741 aaacacacga cacgcccaaa cgcaagaaca caggcaaagc cgcccaaacc ctcgaacatg
    10801 ccgccgcaaa aatgcagagt gccgccgcat acctgaaaca caaaaacccg acagccgccg
    10861 aatttctccg ccacacaagc gaagccgccg cagaactgct cggcagcaaa accgaaaccg
    10921 gagcagccgt acccaacgta cttgccccct atcccgcagc acacctcaaa acactgtccc
    10981 taaaaaacaa cacgaccgtt accattaccc cccttttgcc cgaagacgca gaagccaaac
    11041 agcagttcgt ccgcagcctc agccccgaag cacggtacac acgtttcatg acccacacca
    11101 acgaactgcc cgcagccacg ttggcacgcc tgtgcaaccc cgactaccac tgtgaagccg
    11161 catggacggc aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga
    11221 accgcaacga atgcgaattc ggcatcacac tggcggaaca tatgcgcggc agcgggctgg
    11281 cacagaaaat gatggaactc atcatccaaa ccgccgcaca gcaaggctac cggactatga
    11341 gtgccgacat tctcaaaacc aatactccca tgatcaaact tgctgaaaaa tcaggattta
    11401 ccctcaagga atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag
    11461 acaaaacaac agaaaaaaca aataaaaact tgcacaccga ccacaaaata acctaaaatc
    11521 ggcagtttcc atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc
    11581 acgcggcggc tcgaaacacc gccctttcta caacgtcatc gttactgact cacgcagccg
    11641 ccgcgacggc cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca
    11701 agagcgcgtc cgcctcaacg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt
    11761 cagcgactcc gttgcaaaac tgattaaaga acaaaaagcc gcctaatcca catttgccgc
    11821 catgacagac actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa
    11881 aggctggttg aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga
    11941 gtggcatttg gtcaaggacg gcaaaaccat cagcgttacc cttgaagccg gaaaagtcgt
    12001 caacagcgaa ctccaagtca aattcgaagg cataaacgac cgcgacttgg cattctcatt
    12061 gcacggttac accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata
    12121 ctactggaca gacttggtcg gcatgaccgt cgtcaacaaa gaccataccg ttttaggcaa
    12181 ggtaagcaac ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg
    12241 gcagattctg attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagat
    12301 tattactgcc gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc
    12361 gaaatgttcg acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc
    12421 tggcagtttg aagcagtcaa tccccgaaag tttgccgaca acagattggg ctacatcgac
    12481 gaccgcccgt tcggcggcgg cccgggaatg attatgatgg ctccgccgct tcatgcagca
    12541 atagaacacg ccaaagcaca atcttcccaa gctgcaaaag tcatttacct cagcccccaa
    12601 ggaaaaccgc tgacacaccc aaaagcggca gaactggcag aacttccgca tctgattctg
    12661 ctgtgcggac gctatgaggg aatagacgaa agactgctgc aaaccagcgt cgatgaagaa
    12721 atcagcatcg gagacttcgt tgtttccggc ggagagcttc ccgccatgat gctgatggat
    12781 gcggtattga ggctcgtacc cggcgtattg ggcgatatgc agtctgccga acaagattcg
    12841 ttctcaagcg gcattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg
    12901 gctgttccgg aagtattacg ttccggcaat catggcttga tagcggaatg gcggttggaa
    12961 caatcgctgc gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc
    13021 ccaaaggaat cccgcctctt agaaaccatc cggcaagagc aacgggaaat ccaatcataa
    13081 tttaggaaaa aaacaatgaa cctgattcaa cagctcgagc aagaagaaat tgcccgtctg
    13141 aacaaagaca tccccgaatt cgcaccgggc gacaccgtag tcgtatccgt acgcgtcgtg
    13201 gaaggtaccc gcagccgtct gcaagcctac gaaggcgttg tcattgcacg ccgcaaccgt
    13261 ggtctgaaca gcaacttcat cgtccgtaaa atttccagcg gcgaaggtgt ggaacgtact
    13321 ttccaactgt actctccgac cgtcgaaaaa atcgaagtca aacgccgtgg cgacgtacgc
    13381 cgtgccaaac tgtactacct gcgcggcctg accggtaaag ctgcacgcat taaagaaaaa
    13441 ctgcctgcac gcaaaggttg attcaaaccg ttttccccca atgccgtctg aaccttcaga
    13501 cggcattttt tatcgccgca atatcaacaa gacaacgata ttcccaatcc gcctcccctg
    13561 cttaacaagc actcccgtct attcaaacaa aatttacaga ccaaacgtta tacagtatca
    13621 tagccgccat ataacgataa ccgctttgcc aacttccctt tcaaaaccgc aaatcattat
    13681 gacaccttcc ctcttactgt caggattgac cttccgactc ttcctcgccc tgattgccgt
    13741 atccctttta tggggcgttt acctttgggc ggtatcggta tgagcatcat cgttgaaaac
    13801 ctgaccgtca gctaccgccg ccgacctgcc gtgcaccatg tggacattac ttttgaagaa
    13861 catagtatgt gggcggtttt cggtcccaac ggcgcgggga aatccacctt tctcaaatcc
    13921 ttaatgggat tgcagcctat cgatacgggc agcatccggc tggacggatt gacccgtcag
    13981 aacattgcct accttcccca gcagtccgac atcgaccgct cgcagcctat gaccgttttc
    14041 gacttggcgg caatggggct atggtatgaa atcggttttt tcagaggaat aaatgccgtt
    14101 caaaaacaac gcgttcacga atctttggaa cgcgtcggga tgcggcagtt tgccggccgc
    14161 caaatcgccc acctctcaaa cggacaattc cagcgtgtcc tttttgcccg aatgctggtt
    14221 caaaacgcca aattcctact tctagacgaa cctttcaacg ctgttgatgc acggacgacc
    14281 tacgaacttc tcgacgtatt gcagaaatgc cattgcggcg gacacgccat catcgccgta
    14341 ctgcacgatt acgaacaagt ccgtgcctac tttcccaata ccctgctgct cgcccgcgaa
    14401 aaaattgcgg caggcgcaac cgagaccatt ctgacagaac cctacctcgc ccaagccaac
    14461 gccaaaatgc agcaacagga aagccccgag tggtgcgcct cataaatgcc gtctgaaacc
    14521 atgtccccgt accaaacact atgaacccct acgacctgct tatcacaccc tttgcagaat
    14581 tcgactttat gcgctacgcc ctcgcatccg tcttctgcct gtccctcagt gccgcacccg
    14641 tcggcgtatt cctcgtcatg cgccgtatga gcctgatagg cgacgcattg agccacgccg
    14701 tcctgcccgg tgccgccgtc ggctacatgt ttgccggctt aagcctgccc gccatgggtt
    14761 tgggcggcgt agccgcaggt atgctgatgg cactgcttgc cggactcgtc agccgcttca
    14821 ccaccctgaa agaagatgcc aactttgccg ccttttatct cagcagcctc gccataggtg
    14881 tagtcctcgt cagcaaaaac ggcagcagcg tcgatttgct ccacctcctt ttcggctccg
    14941 tacttgccgt cgatattcct gccctgcaac tcatcgccgc cgtatccacc ctcacactgc
    15001 ttacccttgc cgtcatctac cgcccgctcg tactcgaaag catcgacccc ctgttcctca
    15061 aatccgtcgg cggcaaaggc gggctttggc acgtactctt tctcgtcctg gtcgtcatga
    15121 acctcgtatc cggctttcaa gccctcggca cactcatgtc cgtcggactc atgatgctgc
    15181 cagccattac cgcccgcctg tgggcgaagc acatgggcgc actcatcctc ctatccgttc
    15241 tgacagccct gctgtgcggc ttgagcgggc tgctcatttc ctaccacatc gaaatccctt
    15301 ccggccccgc catcatcctc tgttgcagcg tcctttatct cttttccgtc atactcggca
    15361 aagaaggcgg cattctgacc aaatggctca aaaaccaccg ccaccacacc acctgacccc
    15421 gcaagccgct tgaacaaaca ggcggcttgt tttaccataa cagatgttac gttatatcac
    15481 aggagaacaa ttatgaaaca ccccaaactc acccttatcg ccgcattgct gaccactgcc
    15541 gcaactgccg cccccctgcc ggttgtaacc agcttcagca ttttaggcga cgtagccaaa
    15601 caaatcggcg gagagcgcgt atccatacaa agtttggtcg gagccaacca agatacgcac
    15661 gcctatcata tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact cgtcctgctc
    15721 aacggcttag gacttgaagc tgccgatgta caacgtgccg tcaaacagag caaagtatcc
    15781 tatgccgaag cgaccaaagg catccaaccc ctcaaagccg aagaagaagg cggacaccat
    15841 cacgaccacg atcatgacca cgatcatgac cacgatcatg accacggcga atatgacccc
    15901 cacgtctgga acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg
    15961 ataaaggccg accccgaagg caaagtttat tatcaacaac gtttgggcaa ctaccaaatg
    16021 cagctcaaaa aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa
    16081 cgcaaagtcc tgaccgggca cgatgccttt tcctacatgg gcaaacgtta ccatatcgaa
    16141 ttcatcgccc cgcaaggtgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc
    16201 atcatccgac aaatcaaacg cgaaggcatc aaagccgtct ttaccgaaaa catcaaagac
    16261 acccgtatgg ttgaccgcat cgccaaagaa accggcgtca acgtcagcgg caaactgtat
    16321 tccgacgcac tcggcaacgc gcccgcagac acctacatcg gaatgtaccg ccacaacatc
    16381 aaagccttga ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg
    16441 caaaaggccg tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacaaagccg
    16501 cagacagtac agatagtacg gtaaggcgag gcaacgctgt actggttttt gtgaatccac
    16561 tatattgcta gaagtgcacc gattaaggtt aattaaaata cgacccttat cccaatatta
    16621 agaaaaacag caatcaaaat aaacttgatt gctgtttttc agtttaagcc atgaatgtga
    16681 ttaggctcct tatgcagatg gcgttaccaa taacggctgc tgtacggatt gt
//
LOCUS       DAWKQJ010000032        16546 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000032 DAWKQJ010000000
VERSION     DAWKQJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.032"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            215..781
                     /gene="azu"
                     /locus_tag="WJL20_000769"
     CDS             215..781
                     /gene="azu"
                     /locus_tag="WJL20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ7682945.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPK
                     ASMGHNLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPA
                     KLADGDYKFACTFPGHGALMNGKITLVD"
     gene            874..1383
                     /locus_tag="WJL20_000770"
     CDS             874..1383
                     /locus_tag="WJL20_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7682946.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQVQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGKTFIPKEKLTEIG
                     EIMAARRRR"
     gene            1417..2037
                     /locus_tag="WJL20_000771"
     CDS             1417..2037
                     /locus_tag="WJL20_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ7682947.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVVKWIAETVEDLLPLYEACHGA"
     gene            2137..3282
                     /gene="dapE"
                     /locus_tag="WJL20_000772"
     CDS             2137..3282
                     /gene="dapE"
                     /locus_tag="WJL20_000772"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ7682948.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTGIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGRQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTNVARAAIVETCGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLEDIPKLSAVYEGILARLLAGNAV"
     gene            3653..3932
                     /locus_tag="WJL20_000773"
                     /pseudo
     CDS             3653..3932
                     /locus_tag="WJL20_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(4021..4545)
                     /locus_tag="WJL20_000774"
     CDS             complement(4021..4545)
                     /locus_tag="WJL20_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7682949.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            4856..5608
                     /locus_tag="WJL20_000775"
     CDS             4856..5608
                     /locus_tag="WJL20_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ7682950.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETQVFECEVMPYPNLTEHEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            5799..5874
                     /locus_tag="WJL20_000776"
     tRNA            5799..5874
                     /locus_tag="WJL20_000776"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5832..5834,aa:Asn,seq:gtt)
     gene            6005..7015
                     /gene="waaF"
                     /locus_tag="WJL20_000777"
     CDS             6005..7015
                     /gene="waaF"
                     /locus_tag="WJL20_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ7682951.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTKHFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINRLSDGICVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            7070..7516
                     /gene="smpB"
                     /locus_tag="WJL20_000778"
     CDS             7070..7516
                     /gene="smpB"
                     /locus_tag="WJL20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ7682952.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            7882..9924
                     /locus_tag="WJL20_000779"
     CDS             7882..9924
                     /locus_tag="WJL20_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ7682953.1"
                     /translation="MYYETRSKQASKQKFKKSFIVSLFFSILYTSPLLAVDYVYDNSK
                     LTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALFSKTHQFENINNSKK
                     ISFYDQKYTEDYLVGFARGFGVEKRNGEEEKPLRQYFKDCVNTGNSNNDKCRQPSLGS
                     YAPIRIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNQLSSSQEHLYSDVDPFH
                     YIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKE
                     DYAGGARFRFAYGLKDKVPETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNG
                     TADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLN
                     DKNSDIFDRTLPRKGLWLRVIDGHSKQWVQGKTTPVEGNRKGVQLGGEVFTWQNESNQ
                     LSIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQY
                     QRFRHRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLG
                     VNGKFSDSENAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGV
                     EIDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(10105..11472)
                     /locus_tag="WJL20_000780"
     CDS             complement(10105..11472)
                     /locus_tag="WJL20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7682954.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(11582..11788)
                     /locus_tag="WJL20_000781"
     CDS             complement(11582..11788)
                     /locus_tag="WJL20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682955.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAAASEAPAAEAPAAEAAK"
     gene            12261..12743
                     /locus_tag="WJL20_000782"
     CDS             12261..12743
                     /locus_tag="WJL20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7682956.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12830..13642
                     /gene="hpnC"
                     /locus_tag="WJL20_000783"
     CDS             12830..13642
                     /gene="hpnC"
                     /locus_tag="WJL20_000783"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ7682957.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(13714..14184)
                     /locus_tag="WJL20_000784"
     CDS             complement(13714..14184)
                     /locus_tag="WJL20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ7682958.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            14275..16116
                     /locus_tag="WJL20_000785"
     CDS             14275..16116
                     /locus_tag="WJL20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ7682959.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDIFGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         4108 a         3805 c         4518 g         4115 t
ORIGIN      
        1 taaagaaata ggttcggggt aaaattgccc ttttttgggt aaacgattgt aaacttgcaa
       61 acaggctttg attttaaatg aaatttgtag caaaatgcca ccccgaaaca tctgtttgtg
      121 caacgcggcg gaatcttttt caaggttttg ttaatggcgg ttgcactttg attcctgtaa
      181 aaccgaatat tattttatcg attggagatt tgttatgaaa gcgtatctgg ctctgatttc
      241 tgctgccgtt atcggtttgg ctgcctgctc tcaagaacct gccgcgcctg ctgccgaggc
      301 aactcctgct gctgaagcac ccgcttccga agcacctgcc gccgaagctg ctcctgcaga
      361 tgctgctcct gcagatgctg ccgaagcccc tgctgccggc aactgtgcgg caactgtcga
      421 atccaacgac aatatgcagt tcaacaccaa agacatccaa gtcagcaaag catgtaaaga
      481 gtttaccatc actctgaaac ataccggtac gcaacccaaa gccagcatgg gtcacaacct
      541 tgtgattgcc aaagctgaag acatggacgg cgtatttaaa gacggcgtag gtgctgccga
      601 taccgactat gtcaaacctg acgatgcgcg cgttgttgcc cacaccaaac tgatcggcgg
      661 cggcgaagag tcttccctga ctctggatcc tgccaaattg gctgacggcg actacaaatt
      721 tgcctgcacc ttcccgggtc acggtgcttt gatgaacggt aaaattactt tggttgacta
      781 attcgcctaa agcttgaaaa cagacagcct gccttgagca ggctgttttg ttataatctg
      841 gaaacgttaa tttaagaata aaacctaaaa accatgaatc cgtttgaaac caaaagcgtt
      901 acctttgccg aaccgattga aatgctgtat gcctgccacg gcaaagtgcg ccgtttctgc
      961 ggacaagtcg ccatgctgtc ggactatatc gccgaaaacg gctgcaatca gattgttttg
     1021 caaaccatcc gccaaatcgc ccagtatttc aacgttgccg cgccgctgca ccatgaagac
     1081 gaagaagaaa acttcttccc gctgctgctg caatacgcgc cgcaagtcca agaaagcgtg
     1141 gacgagcttt tgcgtcagca tgtgagcctg tatggcaact gggatgccgt ggctgccgaa
     1201 tttgccaaac tcgaagcaga caacgcttat gtccccgatg aggaagcgtt caaacgtttt
     1261 gtggcgggat atgatgttca tttggcgatt gaagaaccct tatttgacat gggcaaaacc
     1321 tttatcccaa aagagaaact gaccgaaatc ggcgaaatca tggcggcgcg ccggcgcaga
     1381 taatgccgtc tgaaacaatc gtctcaagga tatattatgc tgaccccgaa aagttgcgat
     1441 ttgttcaata tcccgttttt ccaattttcc cagcttaaaa aataccagcc tgaaagcatt
     1501 ccgcaaatca aagccgacta caaggaaaac tggcagatat ggcagcagct gattcagcag
     1561 gttgccgccg aattgggtgc gccgtttgcg ccgccgcaca tcgaacgctg gtgcaacggc
     1621 tggcaggttc gcgcccattt ctttgcctac ttcaaatacg aacaatataa aaattccgcc
     1681 gcgattttgt cgatactgct caaccgccgc cgtttgagtg tcagcttgga ctggcattgc
     1741 tacaaagccg acgtgtcgcc gattgcactg ccggaataca accgctggct ggatgatttc
     1801 gataccgaca aatacgctgc attcgatatg tggcacggtg cggaaagcga atacgacgac
     1861 taccgcaccg tcgcgcagca aaatgaatcc gaccgaaggt tgcaaaacga cgaagatttc
     1921 ttctgcatcg gcaaacacat cgagcgcgac gatttgggca agcaggatgt cgtgaaatgg
     1981 atagcggaaa cagtggaaga tttgctgccg ctttacgaag cgtgtcatgg tgcttgacct
     2041 gaagaacccg cttcgctatc gttagcccgt tccattcaac atcaaatgcc gtctgaaact
     2101 ccgttcagac ggcatctcaa acagaaaagc cgaaaaatga ccgaaaccca atccctagaa
     2161 ctcgccaaag aattgatttc ccgcccatcc gttacccccg acgaccgaga ttgccaaaaa
     2221 ctccttgccg aacgcctgca caaaatcggt tttgcggctg aagaactcca tttcggcgac
     2281 accaaaaaca tctggttgcg acgcggcacg aaagctcccg tcgtctgttt tgcagggcat
     2341 accgacgttg tgccgacggg ccctgttgaa aaatgggatt cgcccccgtt cgaaccgact
     2401 gagcgcgacg gaagattata cgggcgcggc gcggcggaca tgaaaaccgg catcgcctgt
     2461 tttgttaccg cctgcgaacg tttcgttgcc gaacatcccg accatcaagg cagcattgcg
     2521 ctcctgatta cttccgacga agagggcgac gcgctggacg gtacgaccaa agtcgtcgat
     2581 gtgttgaaag cgcgcgacga gcttatcgac tactgcatcg tcggcgaacc gaccgccgtg
     2641 gacaaattgg gcgatatgat taaaaacggc cggcgcggct cgctgtcggg caacctgacc
     2701 gtcaaaggca ggcaagggca tattgcctat cctcatttgg cggtgaaccc cgtccacact
     2761 tttgcccctg ccttgttaga gctgacgcag gaagtatggg acgaaggcaa cgaatacttt
     2821 ccgccgacca gtttccaaat ttccaatatc aacggtggca caggcgcgac caacgtcatt
     2881 ccgggcgagc tgaacgtcaa attcaatttc cgcttctcca ccgagtccac cgaagcaggg
     2941 ctgaaacaac gcgtccacgc cattttggac aaacacggcg tgcaatacga tttgcagtgg
     3001 tcgtgttcgg ggcagccctt tctgacccat gcgggcaaac taaccaatgt ggcacgcgca
     3061 gccatcgttg aaacctgcgg cgtggagacc gaactgtcca ccaccggcgg cacttcggac
     3121 ggacgcttca ttaaagccat tgcgaaagaa ctcatcgaat taggcccgtc caatgccacc
     3181 atccaccaaa tcaacgaaaa cgtgcggctg gaagatattc cgaagctgtc agcggtgtat
     3241 gaggggatat tggcgcggtt gttggctgga aatgccgtct gaaaagaaga tgggctgtcg
     3301 gatacggatg tccgaccatt tgattagacc taacgtgggt ttgagataac gcattgattt
     3361 ttatgtagag cgatttattt caacggcaat ccattccctc tcccgaggga gagggttagg
     3421 gagagggcat tggggcggca acggaaatat tgcctgtatc tgccccgttt taccctctcc
     3481 ccgaccctcc ctcacagggg agggagccaa gttgcaggta cgccgacgga atatggttga
     3541 aacccgtcta ttcggggcgg tgggattttg gtttattttt gggaagtaac cctcccaaat
     3601 cagggtaaac agggctgcgc cttatgtgcg gccctgcgtg ttggagataa tagtgccgtc
     3661 aaattacttt catacgcgcg aacgattggg tgctttgggc ggcatcgtgc aacatcatac
     3721 cgccttgtac cgcagcgtgg cggtatatga accggctgtg cttatcgtgc ggcgcggaag
     3781 caagaagctg cggtgggcga ggcgcgaatt gaggattgcg gcgggtgagg cggtcgcctt
     3841 ggcaggcggg cagacgtttg acgttattaa tattcccgat tcagacggcc tctatcaggc
     3901 gcaatggatt gctttcgaac aggaagccat agaaaggttt gccgcacaat acgggacggc
     3961 gcaggcagtg tgcgatgcgg tgaagctgcc gcaccccggg cggatgggcg cggcgtttga
     4021 ttatgcggcg gcggtgctga cggatgaaga agtgccgcac aatgcggcgg aggtcgcgct
     4081 ttgcggcgtg ttggcgtggt tgcagcatga cggcatcggt tttgccgtgt acgaaggcgt
     4141 caacctgatg cggcaaatcc gcaaactgat taccgccgat atggctgcgg actggtcgtc
     4201 ggcgatgttg gcgcagcggc ttaattgcag cgaggtcgcg ttgcggcggt ggacatcggc
     4261 gggaatgtcg gcgaccaccc ggtgcatcca gcccagtccg gtcggcgcgt ctttatcgta
     4321 aacggtcagg acgaaacttt ttgtcccggc gggcggattt ttccacgaca gcgcgggcga
     4381 agcattgccg cccgaacagc caaatccgta aggcgcgctc aaaagctggt tttgcgtgaa
     4441 gccgccgtct tcggacgggt tgtcgaattg cagcgtgaat gcgcccccgg caaaggcagc
     4501 ggttgaaaaa gccagcagta cggcggagag aatgcgggtt tgcatagatg attcctttgc
     4561 ttgatgaata agtggattca ttataggctg tcaacggcgg caggaatgtg ccgaaacggt
     4621 tggatgatgt gccagaatgc gcaaatgcaa aaatcgtggt ttcagacgag cctttgcagg
     4681 tcgtctgaag tctttaattc ctttccctga ggctttggaa atatcgcata tttaggcatt
     4741 gccgcaacct aaccctcaag atttacgtcg tagcccgttc ctgcatcagc gtgcgggaat
     4801 ggcgaaaatt gggaattttg ccgtcaaatc catgatagag atacggagca aacgaatgat
     4861 taccctgcct tccttaaaca atctgccgtc aaaatgggat gaaatccgct attggctcga
     4921 aacccaagtt tttgagtgcg aagttatgcc gtatcccaat ctgacggaac acgaagccaa
     4981 gcagttcgag cgggattttt gggacaatat cggctgcgcg cctgaagaat atgtccgcat
     5041 ccgccgcgcc atccgtttgt tggaagcgcg ttatcccgac agcttgaacg agctggtgtg
     5101 tgccgccatt gccacgcctt tgggcgagat gctggcggtg ttcggaaata agggattgtg
     5161 tctgctggag tttgtcggac agaagcattt ggaacaggaa attgccgccg tccaaaaagc
     5221 cttgcgcgga cggtttgtgt ttcgggagga tgagcggacg caacttttgc ggcaggaatt
     5281 ggacttatac ttcaagggtc gtctgaaaac ctttgccacg cctttggagc agattggcac
     5341 cgagtttcaa aagcaggcat gggacgcgct cttggcgatt ccttacggcg aaacgcgcag
     5401 ctacaaggag caggcgcagc gtttgggcaa ccccaaagcc gtccgcgccg ttgccgccgc
     5461 caacgggcag aacaaagtat ctatcctgat tccctgccac cgcgtcatcg gcagcgacgg
     5521 caaactgacc ggctacgcgg gcgggttgaa ccgcaaacag tttttactgg ctttggaacg
     5581 cggcgaagtt cagacggcat tgttttaagc gggggagggc agaacggctc atcgggctta
     5641 aatatggtgt gatgacggct gtggaaattc atacaacggc aatcctttta aggcatcggt
     5701 cttaatgtct ttctgattca tatgcttgtc gttttgtcgc ccatttttcc aaaattaagg
     5761 attgacacat tttttccgga cgctataata cgccccattc cccgatagct cagtcggtag
     5821 agcgacggac tgttaatccg caggtccctg gttcgagccc aggtcgggga gccagaattt
     5881 tgaagcccga acttgaatgt tcgggctttt tcatatttgc acgtccggcg ttcggaatgc
     5941 ggcaagatag gttttccaac ggtttcgcgg taaaattctg cctgtctgat ttcggtttct
     6001 tgttatgtcc atcaaaatcc tgattatttc tcccagttgg ataggcgact gcgtgatgac
     6061 ccagcccttg ttccgccgtt tgaaggaact tcatcccggt tgcacgattg atgtgttcgc
     6121 accgaagtgg tcgatggcgg tgtttgagcg tatgccggaa gtgaatgaga ttcttgaaaa
     6181 tccgttcgga cacggtgcgt tggagttgaa acgccgttgg cgggtcggca gggatttggg
     6241 gcggcgcgga tacgatcagg ttattgtgtt gccgggttct ttgaagtcgg cagtcatcgc
     6301 gctggcaacg ggcatcggaa aaagaacggg ttatgtcggt gaaagccgtt attttctgtt
     6361 gaacgatata cgcaggctgg ataaggaacg tctgcctttg atggtggaca gatatacggc
     6421 tctcgcgcat ccgagccagg aggattttga cgggcattcg ggattccccg agttttccat
     6481 tgatgaacgg cggcgggaaa tttctgtcga aacctttggc ttggatattg gaaagcctgt
     6541 tctggctttt tgtccgggtg cggaattcgg gccggcaaag cgttggccga caaagcattt
     6601 tgccgagttg ggcaaacatt attcggcggc gggttggcag gtttggctgt tcggttcgca
     6661 aaaagatgat gaaattgccg aggaaatcaa ccgcctttca gacggcatat gtgtgaattt
     6721 atgcggaaag accgatttgt cgcaggcaat ggatttgctg tcgttggcgg acacggtcgt
     6781 atgcaacgac agcgggctga tgcacttggc ggcggcattg ggcaggaagg tggtggcggt
     6841 ttacggttct tccagcccga cgcatacgcc gcctttgagc gacagggcga aaatcgtcag
     6901 cctgcacttg gaatgttcgc cctgttttaa gcgggaatgt ccgctggggc ataccgactg
     6961 cctcaacagg ctgtatcccg agaagattgt gcaggcggtt gaagaggcgg tatgatgggt
     7021 tctgttttcg ggtttatgcc gtctgacggc ggatattgat aggatgttta tggctattgc
     7081 gaacaataaa aaagcatttc acgatttttt cattgaagac cggattgaag ccggtttggt
     7141 cttggaaggc tgggaagtca aagcaatccg cgccgcgcgc gtacagctta aagagagtta
     7201 tatctattgg aaaaaagacg cgttttatct ggtcggctgc catattacgg ctttgcccac
     7261 agcctcgaca cacattaaac cagatgccgt acgtccgcgc aagctcttgt tgaaccagtc
     7321 ggaaatcaac aaattaatcg gcaaaaccga acgtgccggc tacaccattg tgccgctgga
     7381 tttgcatttt tcgcgcggaa aaatcaagat ggaaatcggt ttggcaaaag gtaagaaaca
     7441 gcacgacaaa cgtcagagta tgaaagaggc ggattggaaa cgcgagaaac agcggttgat
     7501 taagcatacg cgctgattgt ggaaacagtg cttcagacgg tatgggcaag gctgccgcct
     7561 gatgtgtaaa cgttctacac ctaaaccaga atccgcttta atcagcacgg catgatgccc
     7621 caaaccttcg tcaaaggttt gagcaatttg atgataaatt ggcattaacc aagggtaaag
     7681 tgcggtcatt tttcctgaaa tttttgtgaa taacgagggc aattatacac attttcaaac
     7741 tggcttgaca aaatagcaaa aagcaaacgt ttacttttaa taaataagaa ataaatagag
     7801 aaaaatgtga gctaaatgac attttttgtt caattctatg ctaaaatcca cgccccattt
     7861 atctttactt gacaaaggaa aatttattat gaaactcgaa gcaagcaagc aagcaagcag
     7921 aagtttaaaa aatcatttat tgtaagtcta tttttttcta ttctttatac ctctcctctt
     7981 ttggctgttg attacgttta cgacaattct aaattaacaa atgagcaaat tgatcgctta
     8041 aaaaaactta gagataaaag cagcgaatac tggaaacaag aaacttatct tctaaaaccg
     8101 acgaatggta gctctcagaa ctctctcaaa gttcccgcac tattctctaa aacacaccaa
     8161 ttcgaaaaca tcaacaactc aaaaaagatt tctttttatg atcaaaaata taccgaagat
     8221 tacctagttg gcttcgcccg aggtttcggg gttgaaaaaa gaaatgggga agaagaaaaa
     8281 ccactccggc aatattttaa ggactgtgta aatactggga attccaataa tgataagtgt
     8341 agacaaccat ccttaggaag ctatgccccg atacgcataa agagcgatat tttcgcctta
     8401 gcaccacaaa taaaaaatag ccatatcaac agcgaaattt tatctgtagg taattatatc
     8461 gaatggcttc gcccaacact taatcagctt tctagttcgc aagagcatct ctattctgat
     8521 gtagatccat tccactatat tgaggttaca gacaactcgc acgttatcgg gcaaacgatt
     8581 agtttggatg aattccgctt agaaaattct ctctgggagc cccgttggga ttctgatgtt
     8641 ggggaattaa aaacaacgaa tgcagacatc cgttttaata ctaaaagtga aagtttactc
     8701 gtaaaagaag attatgctgg cggagctcgt tttcgttttg cttacggtct aaaagataaa
     8761 gtccctgaaa caccagtttt aacttttgaa aaaaatataa ctggcacttc cgacatcatc
     8821 ttcgaaaatc caatagacga tttaaaaagt ctcgacgggc atcaaattat caaagtaaat
     8881 ggcacagcag ataaacacgc attccgtctt tctggcaaac accaaaaggg aatttatacg
     8941 ctttctttac aacaacgccc agaaggcttt tttaccaaag tgcaagaacg cgatgatatt
     9001 tcgatttatg cacaacaggc tcaagccgcc aataccttat tcgccttgcg tttgaacgac
     9061 aaaaacagcg atatttttga ccgcacttta ccacgcaaag gcttgtggtt gcgtgtgatt
     9121 gacggacatt ctaagcaatg ggtgcaaggt aaaaccacgc cagtagaggg aaatcgaaaa
     9181 ggtgtgcaac tcggcggcga agtattcaca tggcaaaacg aaagtaatca actttctatt
     9241 ggcttaatgg gcggacaggc agaacaacgc agtactttcc gcaacccaga tacagacaat
     9301 cttacaacgg gaaatgtgaa aggctttggt gcaggcgttt acgccacttg gcatcagctt
     9361 caggacaaac agacaggtgc gtatgcggat agctgggtac aatatcaacg cttccgccac
     9421 cgcattaaca ctgaagatgc tacagaacgt tttacttcaa aaggtattac cgcctcaatt
     9481 gaagcgggtt acaatgcttt attggcggaa cacttcacta aaaagggcaa cagccttcgt
     9541 gtttacctac aaccacaggc acaattgact tatttggggg taaacggaaa attcagcgat
     9601 agcgaaaatg cccacgtgaa tttacttggc tcccgccaat tacaaactcg ggttggcgtt
     9661 caagctaaag cgcaattttc gctctataaa aatatcgcta ttgaaccttt cgccgctgtg
     9721 aacgcgcttt accacaacaa acctttcggt gtggaaatag acggagagcg tcgagtgata
     9781 aacaataaga cggcaattga gagccaatta ggtgtagcag tgaaaatcaa atctcactta
     9841 actttacaag caacattcaa ccgccaaaca ggcaaacatc atcaggctaa acaaggtgcg
     9901 ttgaatttac agtggacgtt ttaagttaag ttgtaacaaa ataaaatcgg cagagaaatt
     9961 tactgccgat ttttttattt taaaaatgcg gtcatttttc gtgtcatttc caccaatttt
    10021 ttaccgcact cacgagctcg cgctaaaccg acaatcggat ctattgtccc tttctttgtt
    10081 gtgtatgcaa atcagaaatg ccggttacgg aagcagtttg cccggattca taatgttata
    10141 tggatcaagg tgttgtttga tgcttttcat cagggcgatt tcggcaggcg tgcgtacttt
    10201 gtccagccac tgttttttga tgatacctat gccgtgttcg gcggcaatcg tgccgttgca
    10261 ggcaagaacg ttgcgataaa ctgcgtcgtt gatgtcgttt tcgtaacgat agacttcatt
    10321 gctgaggatt tcgggcagga aagtattgta gtgcaggctg ccgtcgccaa gatgtccgaa
    10381 gcagacgatt tgtatgcctt taaaattctg ttccaaatct ttggcgcacc ggcggacaaa
    10441 gtcggcaacg cgtccgatag gaatggcaat atcgtgtttg atgctggtgc ccagtttgcg
    10501 ttgcgatgcg gagatgtttt cgcgcaatgc ccacatatgg atacgttctt gttcgctttg
    10561 tgccaatacg ctgtcggtaa atcctttttt ataaagaaat tcgacaagcc gatcatcaag
    10621 attgctgtcg ggtaatgagt cggtcaactc aagtaaaata tgccattctg aatgtgtcgg
    10681 cagggggaat ttgctgaatt cggaagacaa ctcggcggca aaacggccga ccagttcaaa
    10741 actgcatagg cgttcggcaa agtgagcttg ggtttcggtc agcaggcgga cggcggattc
    10801 gatgtcgggt atgccgaccc atgccgttgc cttgtccgag ggacgggcaa aaagcttgag
    10861 tgtggcggca gtgatgatgc ccagtgtgcc ttcgctgccg ataaacagat ggcgcaggtc
    10921 gtagccggtg gtgtttttat gcaggggatg gagatgggaa accagttcgc cgttggggag
    10981 gacgacttcc aaaccgataa ccaggtcgcg catcgtgccg taacgcaata cgttcaaacc
    11041 tccggcattg caggcgatgt tgccgccgat ttggcacgag ccttcgctgg cgagactgag
    11101 tgggaacagc ctgtttgagg cttcggctgc ctgttggacg gtttggagta cggaacctgc
    11161 ttcgacggtt atgcagttgt ctgacaaatt gatgctgcgg atgcggttga gtttggaaag
    11221 gttcagcaat acgccgtttt ccgatactgc cgcgccgcac aaaccggtat tgccgccttg
    11281 cggcgtaacc ggaatacggt gtttgtaaca aaaacgcata atggtttgca cgctttcaac
    11341 gctgcgcggc tgcaaaatga tgtctggtgc agacgtaaag cggttgcgct ggtcttttaa
    11401 aagcgtcgga gaaagttcgg caatttcatc tgccggcagg agtcgggaaa attcggtatg
    11461 aaggtcaggc atgtttgtcg gaagggtagg gagaagattg aggaagggaa tctgatgccg
    11521 tctgaaaaat caaaaaagca ggatactgaa cgtatcctgc ttttttgcaa gcggaaaatg
    11581 cttattttgc agcttcggca gcaggagctt cagcggcagg agcctcagaa gcggcagctt
    11641 cagcggcagg tgcttcggta gcggcagctt cagcagcggg agcttcggcg gcaggtgctt
    11701 cagtagcggg agcttcagcg gctttttcac cgccgcaggc tgccaggacc aaagacaaca
    11761 aagcagcggc aaacagggat tttttcattt tagtaacctt tagaaccaat ttaaattatt
    11821 gaaacaaata ggtctttatc gaccatacta caaaccgggt tggaaattca tcgctgaatt
    11881 tgcgtaatta tgcactatgg aataagggat tgcaatgtcg tacagatggg aaaaaggttt
    11941 gaatgaaaag ttttctggcg ggcggattga taaatcctgt aaaacggttt tgctgttaaa
    12001 aattcgatag ggataaatat tatagagatt aatataattt cagtatcttg tatgatttat
    12061 taaggcggat tgggaaagtc ggcagacaga tcggaatggt tttaagaatg ttgtaaaaaa
    12121 gttacaccgc aataaaataa caaaaattta cattgaaata tgtgctgtac agtttatgaa
    12181 caggttaacg gtaatcggcc ggcgcggcgg ttgtgttaga atgcgcctgt aatcaatctg
    12241 ctgttcctga aaggacaaat atggctatcg tgaaaaattc cgtggtttcg ctgcattatg
    12301 agatgtatga tgccaacaat cagcttttgg acaaaaccga agaaccgatt gcgtatctgc
    12361 acggcggtta cgacggcatt ttccctttgg tggaagaggc gttgcacggt aaggatgccg
    12421 gcgatacggt cgatgtggcg ctttcgcccg acgatgcgtt cggcgagcag gattcggagt
    12481 tggttcgtat tgaagatgtg ggcgtgttcc ctgttgaagt cgaagtcggc atgatgtttg
    12541 aagccgacga tcctgaaacc ggcgatgttg tcgtctatcg tgtaaccgat gttgccgacg
    12601 gcaaggcggt ggtggacggc aaccatcctt tggcaggcat gaaaatccgt tttaaggcta
    12661 cggttgaaag cgtgcgcgat gcatccgatg aggaaatcgc acacggtcat gtccacggcc
    12721 cgcacggtca tcaccaccac tgattgccgg cagcttggag gtatctgatg ccgtctgaaa
    12781 agggtttgtt ccgtttcaga cggcattttg tttgatggat ggagaaggta tgtcggttgg
    12841 gcattatgaa aattttcccg tcggttcgct gattttgccg cgcaggttga ggaagccggt
    12901 tcatgcggtg tatgcgtttg cacggacggc ggacgatatg gcggacgagg gcagtatgcc
    12961 gtctgaagct aggttggcgg ggttggaggg tttgcggcgc gagttggacg tgttggcatc
    13021 gggcggccgg tcggcgcatc ctttgattgc acgattggat gccgaggcgg ttgtgccgtt
    13081 cggtttggat ttgcagccgt tttatgattt gctttctgca ttttctcagg atgtggtcaa
    13141 aacgcggtac gcgcatttcg gcgagctggc cgattattgt cggcgttccg ccaaccctgt
    13201 cggacgcatt atgctggctt tgtacgggaa aacggatgcg gtgtgcgtgg cgcaaagcga
    13261 cggcatttgt acggctttgc aactggtcaa tttctggcag gatgtggctg tggattggca
    13321 aaagggcagg gtttatatcc cgcaggacga tttgttgaaa ttcggtgttt ctgaggaaca
    13381 gatcgcggca ggacgggcgg atgcggcgtt tcagcggctg atggcgtatg agtgccgacg
    13441 cgcattccgt atgctgaagg cgggttcgcc tttggcaagg gaactgaagg ggcgtatcgg
    13501 tttggaactc cgtatgattg tgttgggggc gcagttgatt ttgcagaaac tggacgcgtg
    13561 ccgatacgat gtgtttgcac aacgccccgt tttggataag aaggattggt tgattatgct
    13621 gaaaaaggcg ttgtggaaat gattggggga aatgcatgcc gtctgaaagg ggttgttccg
    13681 cttcagacgg cattttgcag gcggttggta tggtcaggcg atttcatgcg ggttcaatct
    13741 ggcttgatct tctgcaccca agccgatgag tttgcgcagt cgggtcatgt agaaattgac
    13801 atccaaaccg gtgccgtagc gttgcgccgt ccacagtgtt tccgccagtg cctccatcat
    13861 ttcgtgttcg gcttccagcc agccgcgttt ggcgcacagg gtgtcgtgga ttgcgcgtat
    13921 gccgtgcggc tggtctatgc ccgcctgttc ttggacggac aaatgcagcg acatatgcag
    13981 gaaggggttg ctttcgccgt tttcgggtag ccagtcggtg tccagatggt cttcgatgcg
    14041 ttcgagataa cggtggtatt cgggatgggc ttcgataatg cggagggctt tctgttccag
    14101 tgcgctcagt tgcagcggat tgagccgctg ctgccacacg cgggcgaaaa agcggcggac
    14161 atcgtgggta ttgacatcgt acatggcggg ttcggtaaga tgtttatgga aataggttcg
    14221 gataatggcg ggttttacat aaattaaatc ccaccgtcaa ccttaccatt tataatgcag
    14281 acaaattatt tgtctgaaac ggattcgatt atgaaaaaac tgatttgcct gttcgccgta
    14341 tttttgatgt tgtgcggacg agctttcgcg ctggatgcga acgatctgct gccgccggaa
    14401 aaggcattcg tgccggagct tgccgttgcc gacgacggtg tgaacgtccg tttcaggatt
    14461 gccgacggat actatatgta tcaggcgaaa atcgtcggca agaccgatcc ggcggatttg
    14521 ttgggacagc cttctttcag caagggcgaa gagaaggaag acgagttttt cggcaggcag
    14581 acggtttacc atcacgaagc gcaggttgcc tttccttatg caaaggctgt cggcgaaccg
    14641 tataaattgg ttttgaccta tcagggctgt gccgaagccg gcgtgtgcta tccgcccgtg
    14701 gataccgagt ttgatatttt cggcaacggc acttaccatc cgcaaaccga cgaaccggca
    14761 tccgccaaag accgcttttt gcagccttcc tctcaaaacg gcagcggggt gctgccgccc
    14821 ccgaaggggg atgagggcgg cgacagccgt ttcaagctgt cttgggatac gctcaacgcc
    14881 aatcttttgg cgttttttct cgccggtttg ggtctgagtt ttaccgcctg tatgtatccc
    14941 ctgttgccga ttgtttccag tattgtggtc ggcgacaaaa aggcgggcaa ggcgcgggcg
    15001 tttgtgctgt ccgtcgttta tgttcagggt ttggctctga cttatacgct ggtcggcatt
    15061 gttgccggac tgacgggcgc actgctgacc gtatggttgc agcaggcttg ggtggtgttg
    15121 gcggcatcgg ctttaatggt cgtcttggca ctgtctatgt tcgggctgtt caacatccag
    15181 cttcccaacg ccgtgcagtc gtattttcag aatcaaagca gcaggctttc aggcggtaaa
    15241 atcgtttccg tctttattat gggcatattg tccgcgctga ttgtcgggcc gtgcgtcgcc
    15301 ccgccgctgg catttgcttt gggctacatc ggtcagacgg gcgatgcggt tttaggcggt
    15361 ttggcacttt acactttggc gttgggcacc ggcgttccgc tgattgccat cggcacgttc
    15421 ggcgggcata tcctgcctaa ggcaggcgat tggatgaatg ccgtcaaata cgctttcggc
    15481 tttatcctgc ttgccgtcgc cgtttacctc gccacgccgc acttgcccta ttatctcgtc
    15541 gtcgcgctgt acacgctgct gatgctggtt cctgcctgta tgctgctggt caacggacgc
    15601 aggcagaaac gccgtccgaa agctgtggca ttcgcattgg gcggcatatt gctgataggc
    15661 ggcgcgtggt tcggctggca gggcgcaaac ggcaaaacga ccgcgctgca ccatttcctg
    15721 accctcaatc cgccggccga agcaggcaaa tcttcggaac acggaaaaat gtttgccgat
    15781 actgccgcgc tgaaggcagc gatggatacg gcgttgaaag aacatcccga caaacccgtc
    15841 gttttggatt tttatgccga ctggtgcatt tcctgcaaag aaatggcggc ttacacgctc
    15901 aatcagccgg aagtgcatca ggcggttgat atggaacgct ttttccaaat cgacgtaacc
    15961 gccaacatgc ccgaacatca ggcgttgttg aaagaatacg gtctgttcgg gccgccgggc
    16021 gtgtttgtcg tccgctccga cggcagccgc agcgagccgc tgctgggttt tgtcaaagca
    16081 gacaagttta tcgagtggta tgaacaaaac cgctgattcc gatgtttcaa atgccgtctg
    16141 aagttctcag acggcatttt tgtcagaaag gaaaacggca atatccacta tacacacgga
    16201 agcagtccaa agatgtgaaa accgtgtgta taagttagtt tgcgtattga aattaaaaga
    16261 taaaaatacg atgattaaaa aataggcata agaaaagata aacacagccg gtgtttgccg
    16321 gtgtgtgtat cttttgtgga aaaacatctt aatgtcatgt attttatgga aaataataac
    16381 atgattaaaa aatagtcaat cattaaggtt caggtagtcg gctgcaaccg tcaatgtctg
    16441 aaaagaggca gtgctgttta agcatggaaa gtcggatata gtggattaac aaaaatcagg
    16501 acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgatt
//
LOCUS       DAWKQJ010000033        15429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000033 DAWKQJ010000000
VERSION     DAWKQJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.033"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(226..549)
                     /locus_tag="WJL20_000786"
     CDS             complement(226..549)
                     /locus_tag="WJL20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ7682960.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(629..1285)
                     /locus_tag="WJL20_000787"
     CDS             complement(629..1285)
                     /locus_tag="WJL20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ7682961.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1387..1890)
                     /locus_tag="WJL20_000788"
     CDS             complement(1387..1890)
                     /locus_tag="WJL20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ7682962.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2074..2847
                     /locus_tag="WJL20_000789"
     CDS             2074..2847
                     /locus_tag="WJL20_000789"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ7682963.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WJL20_000790"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WJL20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ7682964.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPI
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WJL20_000791"
     tRNA            3218..3303
                     /locus_tag="WJL20_000791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="WJL20_000792"
     CDS             4104..4472
                     /locus_tag="WJL20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7682965.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4469..4780
                     /locus_tag="WJL20_000793"
     CDS             4469..4780
                     /locus_tag="WJL20_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ7682966.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5550..6104)
                     /locus_tag="WJL20_000794"
     CDS             complement(5550..6104)
                     /locus_tag="WJL20_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682967.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRKLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(6101..6910)
                     /locus_tag="WJL20_000795"
     CDS             complement(6101..6910)
                     /locus_tag="WJL20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7682968.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            complement(6903..7715)
                     /locus_tag="WJL20_000796"
     CDS             complement(6903..7715)
                     /locus_tag="WJL20_000796"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ7682969.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KKLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            complement(7950..10580)
                     /locus_tag="WJL20_000797"
     CDS             complement(7950..10580)
                     /locus_tag="WJL20_000797"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ7682970.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYANFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAAGKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10850..11365
                     /locus_tag="WJL20_000798"
     CDS             10850..11365
                     /locus_tag="WJL20_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7682971.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11372..11815
                     /locus_tag="WJL20_000799"
     CDS             11372..11815
                     /locus_tag="WJL20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ7682972.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11984..14041
                     /gene="ppk1"
                     /locus_tag="WJL20_000800"
     CDS             11984..14041
                     /gene="ppk1"
                     /locus_tag="WJL20_000800"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ7682973.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRAGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASVAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGSYIRAAPAEGESEVDLQNDLWDL
                     LGG"
     gene            14066..14335
                     /locus_tag="WJL20_000801"
     CDS             14066..14335
                     /locus_tag="WJL20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682974.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYPSDSRTKPRIRLHGSGHISNTGIALRKTVKSASASQYI"
     gene            complement(14304..15407)
                     /locus_tag="WJL20_000802"
     CDS             complement(14304..15407)
                     /locus_tag="WJL20_000802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ7682975.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT         3850 a         4261 c         3790 g         3528 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa accttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcacccccca aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaaccc
      601 gctttcagac ggcatcgcaa tccgaacatc aaaactgaaa accgccaaac ggatcggcat
      661 ccttatcatc caaatgcgcc accaaggtat cgaacagcac cttctcttca aacacatcgc
      721 ccttgcgcgt aatcaaaagc gcgcgctgaa caggcttgcg gcctacgata accaccatgc
      781 gtccgccatc tttcaactgt tctttcaaca cttcaggcac aaggtttacc gcaccgccga
      841 cataaaccgc atcaaacggc gcacctgcgg aaagttcggt caacccgttg ttttgcacat
      901 aatcgatatt gtccaaaccc aagccgtcca acaccgcttt ggcgcggttt tgctgttcgg
      961 catcgatgtc gtccgacacc acacgaccag ccaattttgc caacagcgcg gtcgcatagc
     1021 ccgaacccgt gccgatttcc aaaaccgtat cgtttttcgt cagcttcaag ccctgcgcca
     1081 gccgcgccac gactttcggc tcgagcatct tatgaccgtt ggcaagcggc agcgccatat
     1141 ccgcatacgc caaaccctgc aagtcctcat cgacaaaaag ctcgcgcgga atctccgcca
     1201 aagcgtccaa aacgtcaaaa tccaatacat cccaaggacg gatttgctgt tcgaccatat
     1261 tgaaccgcgc tttttcaaaa tccatttagt gctccgtcaa ataattgttc caacaatagc
     1321 aggcattata aaaccacact cccgccgcgc caacttcggc acgcgccgcc cgcccctttc
     1381 cctcactcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagctc
     1441 gcccaaatac aaagcatctg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gcgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtc cacatccccg acgaccaccg tcccgccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgtctcgtcc gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccaaaaca acattccaaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc accaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccagaacaa caattcgctc
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc gacaacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctgcaagggc tggataccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcgatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac cttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgt tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacga caaaatacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccataa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt tttttttggg tttttggaaa aaacctatac tatagtggat
     3661 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat ttaattcact
     3721 ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgg taacgaatcg
     3781 gtaacaactt ttggggtttt ccggtttttc accgtcttgg cagtgggagc gtagcggaat
     3841 gaaaagccaa aacgcacgga accgcgccta ttttgagcag gaatggcggt taaaccgctt
     3901 ggttatatac ggggaatagg aagacagcga aacgcgctaa aaacgcaatt tgagccatta
     3961 aaagcgattg attaaaaaat aatcaggtta gccgccagtg tttcaggcgg cataaacgga
     4021 gaaattgcag ggtataaaaa ggcggcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaaaca taaaatcagt gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaatcat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggagaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cacgctgctg aatatgcctg ccaaaacagg caagaccgcc
     4861 gaactgaata tcagggcgta gccgcataaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcgacggtgc agacgacata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgagccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaaag caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga tccccttccc
     5161 ttaacgccga cgttttttat taacgcgccc cttatttctg acactttgct cataaacagg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgttta cagcatcagc
     5281 ataagacacc gtactctgga ataccgccta aaccctgcgg atagttttgg atgtggtatc
     5341 gacattgtcg gcagacttgt ctaactcgac cgttttcaaa taatctttga cagatgctgc
     5401 attcagtgtc gcggaatccg cgtattttga acaaaaggtc gggctacttt atcacaatag
     5461 cagaaagatg gaaaacggcg gggattggtg aaaggtcgtc tgaaatgata ttcagacgac
     5521 cttgaacggc tgtgttttaa atatggtatt tattcatttg tttcaccatc aggcagtcca
     5581 aaccacctta agggaatcgt gttaaattgg taatggcagt taacactgat aatataatct
     5641 aaaacatctt tatattccgg ctttgtaaac caatcattca aaacatagac atattcaacc
     5701 cgccaaccta aagagtgaac caattttgta tattgttttc tcttaaaatc gcaagtttgt
     5761 aatttctcat ctactgaacc gggagtttgt tggaatttga tctcaataat aaataaggta
     5821 tcacggatga tgataaataa gccattgtcg ggtagtaatc ttttagacag atggtctttc
     5881 caattaatac tgtatcccgg tcttttcaaa aatcggtaaa actcatattt tttaaaacaa
     5941 taagccttca attctccatt gaaccaaact tcataacccg cattatcata tgtttttctt
     6001 aagtcgtacc cgggaatttc ttcaaacaac tttcgtaagt ctgtacgttc ttcgaatctg
     6061 agtcctgttt gtgtccttgc accaccgatt ccgccaataa tcatttttct aacctttttt
     6121 ttgctaattc aaaaaaatct tcttccagtt cgcaaccaca aaatttccga ccatgtttta
     6181 aggcggcaac gcctgttgtg ccgctgccca tgaaagggtc aaaaattaaa tctccgatgt
     6241 ttgaagctga gagtatgcag cgttcaagca aggggagtgg tttttgtgtc ggatgtttgc
     6301 cgaatgtttt ttcggctttg tttggaggag gaaatgtcca aacacatttc atctgtttgc
     6361 catcattttg tgctttcatc atttcataat gaaacgtgtg tttggctttt ttatttttct
     6421 ttgcccataa gattgtttct gtcgaatggg taaaaaaacg gcaggacaaa ttagggggag
     6481 gattgggttt ttcccaagta atattgttca aaatatggta tccgatagtt tgcatcaggt
     6541 agccgattaa ataaatatta tggaatgtgc cgcaaaccca aattgtgccg tttggtttta
     6601 ataaggcgta acacagtcgg agccattctt cgtagaattc caaatctgcc gccattcctt
     6661 tggatttatc ccagttgccc ttattgacgg aaaccatttg tccgttttga caactgaagc
     6721 cgtcattgga aagaaagtaa ggcgggtctg cgaaaatcat atcaaaacag ccgtttggat
     6781 atttttccaa tatcttccgc atgacgttaa gggaattttc gttgtagagg acaaggtcgt
     6841 ctgaaacgat ttttttcaaa cgactttgat aaatggcgtc ttcttcagcc ggtttgtcaa
     6901 aatcaatcat aaattgccaa taattctccg acttttttac ggctgctgcc ttttgcggca
     6961 atattgcgtt gtacttcaat tgttttcaga atagcaaaag aataaatatc ccttgtgaat
     7021 tcagtatcat gatttgaaat caaaacgcct tgggaatcgg gcgcaatttg ttgggctgac
     7081 tgggcaagac ggatttgatt gtctaaattg aaaccttctt tggcataagt ggtaaatgaa
     7141 gcggttgggc ttaaaggcgc gtaaggggga tcgcaataaa ccgtatcggt attgtctgtc
     7201 tgagatagaa tcatttgaaa atctccgcac attaattcaa ctctgtcaga tttctgaatg
     7261 aaaccttcca tttcttgttg gggaaagtac ggcgatttgt agcgtccgaa cggcacattg
     7321 aatacccctt tgctgttgta acggcataaa ccgttaaaag catgacggtt caaataaata
     7381 aatagtgccg agcgttcgtg cagctttttg ctgaagttga attgttcccg caattcatag
     7441 aatcttgaat cgctattgtt ttcggaaata aagaaagagc gggcgtagtc gatgaatccg
     7501 gatttttctt cttttaggat acggaataag ccgattaagt cagcgttaat atcattgagc
     7561 agatattgtt cgaaatccaa tgcaagagac aaagcggcag aaccgcaaaa gggttcaatc
     7621 aggcgttttc gtgtgggagt aggtagattg tgttcgataa aggggacaag tttatgtttg
     7681 ccgcctgccc attttagaaa aggttttgaa gccattagga tgccgagtac aagcgtggtt
     7741 gattggcttg gaattgtgcc aaaacatcgt gaggaataca atggatttat atttgggggc
     7801 gtgtgtagag tgagtctaaa tccaatagaa cctttccatt ccaatatgtt attggactta
     7861 tcaactccat ctgccttaaa aaggccgtct gaaaccctgt ttccaagttt cagacggcct
     7921 ttatccgtgt ggctaaacct taaaagcggt tagacgacga tgttcaccag tctgcccggt
     7981 acgacgatga ttttcttcgc cggtttgcct tccatgaatt tcaccgcgcc ttcggtggcg
     8041 agtgcggcgg cttcgaggtc ggctttggat gcgtcggcgg caacagtgat tttgccgcgc
     8101 agtttgccgt tgacttgaac catcacttcg atttcggatt tgaccaaggc ggcttcgtcg
     8161 actgtcggcc agcctgcttc ccacagtttc gcgccgttca attcgctcca cagggtttcg
     8221 cagatgtgcg gcacgatggg ccacaacagg cgtacggcgg tttccaatac ttcttgggcg
     8281 acggcgcgtc cttgttcgcc gccggtgtcg gttttgtcgt attggttgag caattccatc
     8341 acggcggcga tggcggtgtt gaactgctgg cggcggccgt agtcgtcgct gactttggca
     8401 gtggtcgcgt gcagtttgtg gcgcaggtct ttgagttctt tagacaaacc gtcttggctg
     8461 cctgcgaacg ctttgaccgc ttcgccttgc ttcaagtatt cgtaaacggt acgccacagg
     8521 cggcgcagga agcggtgtgc gccttcgacg ccgctgtcgc tccattcgag ggactgttcg
     8581 ggcggtgcgg cgaacatcat aaacaggcgg gcggtgtccg cgccgtaggc gttaatcagt
     8641 tcttgcggat cgacgccgtt gtttttggac ttggacattt tttccgtgcc gctgatgacg
     8701 acgggcagcc cgtcggcttt gaggacggcg gaaatggggc ggcctttgtc gtcgaacgtc
     8761 agctcgacat cggcggggtt gatccaatct ttgccgcctt tgtcgttttc gcggtagtag
     8821 gtttcgcaaa cgaccatgcc ttgcgtcagc aggcgttcaa acggttcgtc aacattgact
     8881 agaccttcgt cgcgcatcag tttggtgaag aaacgcgcgt acaagaggtg caaaatcgcg
     8941 tgttcgatgc cgccgatgta ttggtcgacc gcgccccagt atttcgcggc ggcaggatcg
     9001 accatgccgt ctgaaaattt tggcgacatg tagcggaaga aataccagct cgattccatg
     9061 aaggtgtcca tggtgtcggt ttcgcgtttc gccgcgccgc cgcagcatgg gcaggcagtt
     9121 tcgtaaaact cgggcatttt tgccagcggc gaacccatgc cgtcgggtac gacgttttca
     9181 ggcaaaacga ccggcaattg gtcggcaggg acgggtacgt cgccgcattg ttcgcaatgg
     9241 acgatgggaa tcgggcagcc ccagtagcgt tggcgcgaaa tgccccagtc gcgcaggcgg
     9301 tattgggttt tcggttcgcc cgcgccttgg ctttgcagct tggcggcgac ggcgtcgaat
     9361 gccgtctgaa aatccaagcc gtccaagtcg ccgctgttga ccaatacgcc gttttctttg
     9421 tcgccgtacc attcttgcca ttggttttcg tcaaatgcgt tgtcgccgac ggcaatgact
     9481 tgttttttcg gcagattgta tttggtggcg aactcaaaat cgcgttcgtc gtgcgccgga
     9541 accgccatca ccgcgccgtc gccgtagccc cacaatacat agttggcaat ccacacttcc
     9601 agcttgtcgc cgttgagcgg gttgacgacg tagcggccgg tcggcacgcc ttttttctcc
     9661 atcgtcgcca tatcggcttc ggcaaccgaa cctgctttgc attcggcgat aaatgcctgc
     9721 aattcgggtt tgccggcggc tgcggcggtt gccagcggat gctcggcggc aacggcgaca
     9781 taagtcgcgc ccatcagcgt gtcgggacgg gtggtataaa cttgcaggaa gttcgcgtaa
     9841 tcgccttcca agccttgttt gctgtcgtct gaaacagcaa agcgcacggt catgccgcga
     9901 gatttgccga tccagttgcg ctgcatggtt ttgacttgtt caggccagtg ttccagcttg
     9961 tccaagtcgt tgagcagctc ttcggcgtaa tcggtgattt tgaagtaata catcgggatt
    10021 tcgcgttttt cgatcaacgc gcccgaacgc caaccgcgtc cgtcgataac ttgctcgttg
    10081 gcaaggacgg tttggtcgac cgggtcccag tttaccgtgc cgtttttgcg ataaacgatg
    10141 cctttttcaa acagcttggt aaacagccat tgttcccagc ggtagtattc gggtttgcag
    10201 gttgcggttt cgcgcgccca gtcaatcgca aaacccaggc ttttgagctg ggttttcatg
    10261 tattcgatgt tatcgtacgt ccaagcggca ggggcgacgt tgtttttcat cgccgcgttt
    10321 tccgccggca tgccgaacgc gtcccaaccc ataggctgca tgacgttgaa gccgtttaaa
    10381 agtttgaagc ggctcaatac atcgccgatg gtgtagttgc gcacatgccc catatgcagc
    10441 ttgccgctgg gataggggaa catggagagg caataatatt tgggtttgga agcgtcttcg
    10501 gagacgttga aaatacgggc gtcgtcccat tttttctgcg ccgcaggctc gatggcggcg
    10561 ggttgatatt gttcttgcat agtcattctg ttttcgctta aaaacgttgg aaaaataaag
    10621 tcggcatcaa ttataacagg ttgccggaag cggcgaatcg gcagattgcc ggcaggatgc
    10681 gtaaattcgc acgcgcatta ttccgtatgc cgtacaaata caccgcgttt attgatacgc
    10741 acgtttttta tgctaatatt acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc
    10801 tcttccgaac aaaaggcaga cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa
    10861 acaaatcgtc aaaccgggct tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac
    10921 caaaagcaac gtcaaagccg acggcacgac cgacaatccg gttttcccga aaccctattc
    10981 cgtaacgctc gacaacaagc gcggcacatt cccgacttat gacgaactgg atcagatgcg
    11041 ccccggcctg accaaagacg acatctacaa aatcctgggc cgcccgcatt acgacgaagg
    11101 tatgtacggc gtgcgcgaat gggattacct gttccacttc cataccccgg gcgtaggtat
    11161 cgaccctgaa aacacttccg gcgtagaaga tgttactacc tgccaataca aagtgatttt
    11221 cgataaagac aaatttgccc gcagcttcta ctggaacccc gtcttcccga aagatgccgc
    11281 ctgtccgccg cccgcaccca aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc
    11341 aaaaccgaaa cgtatccgcc aataatccga catgccgttc cgcctgtttt tagggatatt
    11401 atgcggcctg tcaatggttg cccccgtata tgcacagggg caaccggatg cgatcaatgc
    11461 ctatatccaa aagaaaaaag tcatcgtcga tacatccaaa gcggaactct gtttcgctga
    11521 cgaccgtcag tgccaccccg tcctcatcgg tgttgccacg cccaagggga cgttcgggct
    11581 gacgctgaac agtaccgaca agcctggata cggcggcgaa gtcatcggtt tcaagcagga
    11641 gggtgatttt cttttcgccc tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa
    11701 cgaacgcatc gcctccccgt ccgtgtccga caggattatg accaacggct gcatcaacgt
    11761 cagcgatgcg gtgtacgaaa aactgcgtca ttattttgtg ttggaagtga tttgaaacag
    11821 acggataccg cacgcgccgg tatctgtttt cacattgccc cgatgcctga aacagactgt
    11881 ccgccacgtc atgccgtctg aaaccggcgc agatgccgcc aagccttcag acggcattgc
    11941 ctgcccgctc cgaccgaaca acaaccatct ttgggagaac cttatgcccg aacaaaaccg
    12001 catcctctgc cgcgaactaa gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga
    12061 agacaaaaac gtcccccttt tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct
    12121 tgacgagttt ttcgaagtcc gcatggcgtg gctcaagcgt gaaaacaagc tgcacccccg
    12181 gcgcaggctg gacaacggca aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc
    12241 gcgctccctg atacggcatc agtacgacct gttcaacaac gtccttcagc ccgagttggc
    12301 gcgggaaagc atccattttt accgccgccg caactggacg ggcacgcaga aaaaatggat
    12361 cgaagactat ttcgaccgcg aactgctgcc gatcctgacc cccatcggac tcgacccgtc
    12421 ccaccccttc ccgcgaccgc tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac
    12481 ggacgcattc ggcaggcctt cggggatggc gattgtgcag gcaccgcgca tcctgccgcg
    12541 cgtcgttccc ctgccgtccg aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc
    12601 catcctccac gcccacgtcg gcaaactctt cccgggcatg aacgtcaaag gctgccacca
    12661 gttccgcctg acgcgcgaca gcgacttgac cgttgacgaa gaagacctgc aaaacctccg
    12721 cgccgccatc caaaacgaac tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt
    12781 cgccgacacc tgtcccgcct acatccgcga ctttctgctc gcgcaattca aactgaccgc
    12841 cgccgaactc tatcaggtca aaggcccggt caacctcgtg cgcctcaacg ccgtccccga
    12901 cctagtcaac cgccccgatt tgaaatttcc cacacacacg ccgggcagac tgaaagcctt
    12961 gggcaaaacc gcgtccatat tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc
    13021 ctaccaatcg ttcgaccccg ttgtcgaaat gatgcgcgaa gccgccgccg atcccgacgt
    13081 gcttgccgtc aaaatgacga tttaccgcgc cggcacgcgc tccgaactcg tccgcgccct
    13141 gatgaaggcg gcactcgccg gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt
    13201 tgacgaagcc aacaacgtca actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt
    13261 gtacggcgtg ttcggctaca aagtccacgc caaaatggca ctggtcatcc gccgcgaaga
    13321 cggcgtgctc aagcgttacg cccacctcgg cacgggtaac taccaccaag gcacatcgcg
    13381 catctacacc gacttcggcc tcatcacagc cgacgaacaa atcaccgccg atgtgaacac
    13441 attgtttatg gaaatcacag gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag
    13501 cccgtttacc ctgcacaaaa tggttatcga ccgcatcgca cgcgaaaccg aacacgcgaa
    13561 agccggcaaa cccgcccgaa tcaccgccaa gatgaattcg ctcatcgaac cgaccgtcat
    13621 cgaagccctg tatcgggcaa gcgtggcagg cgtgcaaatc gatttgattg tgcgcggtat
    13681 gtgcaccttg cgcccgggtg taaaaggctt gtccgaaaac atccgcgtcc gctccatcgt
    13741 cggcaggcag ctcgaacacg cgcgcgtgta ttgcttccac aacaacggcg cggacgatac
    13801 ctttatctcc agcgcggatt ggatggggcg caacttcttc cgccgcatcg aaaccgccgc
    13861 gccgattacc gcgcccgaac tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcact
    13921 ggcagacaat acgcacgcat ggctgatgca gccggacggc agctatatcc gcgccgcacc
    13981 tgccgagggc gaatccgaag tcgacctgca aaacgatttg tgggatttgc tcggaggctg
    14041 acccgtaccg ccccaatcaa aaaccatgcc gtctgaaacc tttccgtttc agacggcatg
    14101 gttttacagc aatctaaaca gggcggaccg gagtcaaaaa cacaccttcg ccattcctgc
    14161 acaagcactt cccctatacg ctcccaaccc caagccgccg cattccagac ggcattaccc
    14221 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tttccaatac
    14281 gggcatcgcg ctccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
    14341 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
    14401 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
    14461 agttcgccgc cctgataggc gatttcgatt tcttcgcgcg cttcttcctg ctcgttgttg
    14521 ctgcacacaa cgctcaacag gccgggttgc aggaacaggc gcgcgccacg gaatttttcg
    14581 ttggcaagaa tggacacacg ttccagcgca cccaaaagtt cggcacggga caaaacgaaa
    14641 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
    14701 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
    14761 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
    14821 aaaatcactt cggcgtgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
    14881 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
    14941 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
    15001 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
    15061 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
    15121 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc
    15181 tgaaatttct tggcattggt agtgatgcgg aagtcgcccg cgccgccctc gggacccgca
    15241 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
    15301 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
    15361 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatgtt cgctccttta
    15421 aatcggttt
//
LOCUS       DAWKQJ010000034        15138 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000034 DAWKQJ010000000
VERSION     DAWKQJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.034"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..479)
                     /gene="rpsI"
                     /locus_tag="WJL20_000803"
     CDS             complement(87..479)
                     /gene="rpsI"
                     /locus_tag="WJL20_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ7682976.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(489..920)
                     /gene="rplM"
                     /locus_tag="WJL20_000804"
     CDS             complement(489..920)
                     /gene="rplM"
                     /locus_tag="WJL20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ7682977.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1098..1277)
                     /gene="ssrS"
                     /locus_tag="WJL20_000805"
     ncRNA           complement(1098..1277)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WJL20_000805"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1283..1588)
                     /locus_tag="WJL20_000806"
     CDS             complement(1283..1588)
                     /locus_tag="WJL20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ7682978.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1599..1928)
                     /locus_tag="WJL20_000807"
     CDS             complement(1599..1928)
                     /locus_tag="WJL20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682979.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(1983..2972)
                     /locus_tag="WJL20_000808"
     CDS             complement(1983..2972)
                     /locus_tag="WJL20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ7682980.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3095..5797)
                     /gene="ppc"
                     /locus_tag="WJL20_000809"
     CDS             complement(3095..5797)
                     /gene="ppc"
                     /locus_tag="WJL20_000809"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ7682981.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5840..6733
                     /locus_tag="WJL20_000810"
     CDS             5840..6733
                     /locus_tag="WJL20_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ7682982.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALADRLHHINHTVDVRTVNEKLDSGRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(7133..7357)
                     /locus_tag="WJL20_000811"
     CDS             complement(7133..7357)
                     /locus_tag="WJL20_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEZ7682983.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIARLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7358..8746)
                     /locus_tag="WJL20_000812"
     CDS             complement(7358..8746)
                     /locus_tag="WJL20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ7682984.1"
                     /translation="MNAVVVAVIVMLSLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8832..9653)
                     /gene="prmC"
                     /locus_tag="WJL20_000813"
     CDS             complement(8832..9653)
                     /gene="prmC"
                     /locus_tag="WJL20_000813"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7682985.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(9643..10104)
                     /locus_tag="WJL20_000814"
     CDS             complement(9643..10104)
                     /locus_tag="WJL20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682986.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(10169..11653)
                     /gene="tldD"
                     /locus_tag="WJL20_000815"
     CDS             complement(10169..11653)
                     /gene="tldD"
                     /locus_tag="WJL20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ7682987.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      11728..11831
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11898..13121
                     /gene="cytX"
                     /locus_tag="WJL20_000816"
     CDS             11898..13121
                     /gene="cytX"
                     /locus_tag="WJL20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ7682988.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            13118..14218
                     /locus_tag="WJL20_000817"
     CDS             13118..14218
                     /locus_tag="WJL20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7682989.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQPPEYTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            14239..14856
                     /gene="thiE"
                     /locus_tag="WJL20_000818"
     CDS             14239..14856
                     /gene="thiE"
                     /locus_tag="WJL20_000818"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ7682990.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIALCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3146 a         4287 c         4242 g         3463 t
ORIGIN      
        1 atctgtaatc tttagattgc caatgggaaa ccgtctacta caaataaaaa accctgcgat
       61 aagcagggtt ttttgaattt ccaacattaa cgtttggaga attgttttgc acggcgtgct
      121 ttgcgcagac ccggtttttt acgttcgact tcgcgggcat cgcgggtaac aaaaccagct
      181 tgagacaagg cgggtttcaa cgcggcatcg aagtcgatca gggcacgggt aatgccgtgg
      241 cggattgcgc cggactggcc ggtttcgccg ccgccaacaa cattgacttt gatgtcgaaa
      301 gattcggcgt tttcagtcag aaccaagggt tggcgaacaa ccattcggct ggtttcgcgt
      361 gcgaagaatt cgtcaacggg acggccgttt acgatgattt gacctgtacc tttaatcagg
      421 aatacacgag ccactgaact tttgcggcgg cctgtgccgt agtagtattt accgttcatg
      481 tcgcgtcctt atttcagttc caaaactttg ggttgttgcg cagcatgggc gtgttccgca
      541 cccgcataca ctttcagttt tttaatcatg gcgtaaccca aaggaccttt gggcagcata
      601 ccttttacag cttgttccaa agcgcggccc gggaattgct cttgcatttc gcggaaagtg
      661 cgttcgtaga taccgcctgg gaaaccggaa tggcggaagt attttttatc ttcgaatttg
      721 gcaccggtta cacgcagttt gtccgcattg ataacaatga tgtaatcgcc ggtatcgacg
      781 tggggggtgt attcaggttt gtgtttgcca cgcagacggc tggcgacttc ggccgcaacg
      841 cgacccaaga ctttgtcttg ggcatcgatg acgaaccatt cgcgcttcac ctcgtggggt
      901 ttcgctgaaa aggttttcat agtggaaatc cagatagata tagaaagttg taaattttaa
      961 agacaggatt cgattttgtc aatcgcatta ccgcgttacg gaaggatttt ccggatttcg
     1021 gcagactgca tactgctttt tcgggcgggg cggcggccaa tgtgaaaaac cgcatcgttg
     1081 cgatgcggtt ttgaatggga atccccgcga gagccgtttc ggccgaatcc gcttgaacct
     1141 tgctgacaag gcggctgcct cgggtagttt cgggtgcgtc cgcaaaagga cgctcgcgcc
     1201 cactactgct cccggcaacc ttaagcgaac ttattggttc aaaggaatat atgccttcgc
     1261 ggacaccgca gggaaaaagg ggttattcct gcgccaagcg ggatagtgct ttttggcagg
     1321 cgttgtccat atcggctatt ttacgcgcaa aatcgccgat tgccaaatcg ccgccgttca
     1381 gggaggtttt caacaggtcg tggacgacgt tgagcgcggc cataatgacg attttttcgc
     1441 tgtccgcgac gcgtccgcct tcgcggatgg cttcggcttt gccgttgagc attccgactg
     1501 cctgcaacag tgtgtctttt tcttctgccg gcgtgttgac ggtcagccgg gcgtgcatga
     1561 cttcgatgtg gacttgttcg atgttcatcc tttaatcctt attgctgcgt ttcctgccat
     1621 tgggggaggc gcgctgccag tgcgctgatt ttttccctgc tctgttcgag caggctgcgg
     1681 tatcgtgtat tttcttctgt caggctgtca attttgtttt gcaggtcttc tttgagtttg
     1741 ccgacttgga cgagcagggc ttcgctgagt tcgtcgacgg cggtttcgtg ttcgagtttt
     1801 tgccgctcgt gcgcccgttt gagttcggcg acggtttctt tgaggcggcg gttttcgctg
     1861 acgagggttt cgaatttttg taccagcgta taaacgctgc tttcgagttt ttcgatattt
     1921 tgtttcataa ccttacctgt ccgtatgccg tctgaaggcc tcagacggca tctgtctgtt
     1981 gtttattcga aacgcgcgct gcgttccatc agtctttcga cgacctgctg aggggtcatt
     2041 tccttacgga tgagttgcag cagggtttgg gtaatcggca tatcgatttg gtacttacag
     2101 gcagtattga agacttcttc tatcgtgctg accccttcgg aaacgtgtcc gatttcgacc
     2161 agcacctgat gcagttcctt gccttctgcc aaacccaagc cgacgcggcg gttgcgtgag
     2221 agtgcgccag tgcaggtcaa gatgaggtcg ccgatgcctg ccagccccat catggttttg
     2281 ggctgtgcgc ccattgcgga ggcaaggcgg gtaatttcag ctaatccgcg cgtaaccagt
     2341 gcggcacggg cgttaagccc gtactctagg ccatcggaca atccggtggc aatcgccata
     2401 acatttttta ccgcgccgcc aaccgccacg ccgataacgt cggtactgcc gtaaagcctc
     2461 atgacagtcg tgttgagctg cggtacgagt tcttcaatcc actcttggtt ttcggaggcg
     2521 aggacgacgg cacagggcag ttgtttggcg agttcctgtg caaaactcgg gccggagagt
     2581 acgccgattt tcttattgtc gggcaatact tctttcaata cttgaaaggt cagcagccct
     2641 gtatcctgct cgaatccttt gcaggcggcg aggacgggga ggtgtcccgc gccgtactgt
     2701 ttgagcagct ctgcgctgct tctcaatccg gcaacggagg ttacgataag gacaagtccg
     2761 ctgtctttga gcgcgtctgc caaatccgca cacacttcca aggtttcggg aaaggaaaag
     2821 ccgggcagtc cgcgtttgtt ttcacgcgct tcctgcattt ggcggacttg gtctgcgttg
     2881 cgcgtccaca gggatacgcg gttgccgtgt tgggaaaagt gcagggcgag cgccgtaccc
     2941 cacgaacctg cgccgataac ggtaattttc attggtcgtc tttcaacata tcactgccgt
     3001 tcactttaaa acaatcggtg tttctctgca agtgcggtca gggaaatgcc gtctgaaagg
     3061 tgttcagacg gcattttgcc ccgatgcggc acaatcagcc tgtattgcgc aaaccttgcg
     3121 ccacgccgtt gatggtcagg tgaaccatca gcagcgcgtg cggattgtcg ggttctttac
     3181 gcaggcgttt gagcatggcg acctgcaggc cgttgagcgc gttcaaatat ggaatcctca
     3241 aggcaagcga acgggcgagg ctgcggttgt cgcgtaaaag ttcttcggtt tgcagcaggt
     3301 cgagcagggc tttgcggctg cgttggtatt cttccttaat catgccgaag atgacggcgg
     3361 ctttttccgg cgattcgctc aagccggcat agttttccgc gagggtgatg tcggttttcg
     3421 ccatcacttg ctccatattg gaaagcatgg cttggaagaa cgggttgctt tgggcgtgtt
     3481 cgcgcagggc ggcgagcgtt tcgggtttgt cttcgcacaa ggtttccacc gcgctgccga
     3541 aaccgtacca agcaggcagc atgaggcggt tttgcatcca tgagaatacc cacggaatag
     3601 cgcgcaagtc ctgaatccgc gccaaggttt tgcggctggc aggacggctg ccgaggttga
     3661 gggtggcgat ttcctgaatc gggctggttt gcaggaagta gtcgatgaag tcggggtggg
     3721 taatcagttc gcggtagtat ttgaacgata cgtccgacaa tgcctgcatc agtttggcat
     3781 cagggtcttt tttatccggc aggatgctgg cttccaaagt cgcggcaacc aaggtttcca
     3841 agttgcgttg ggcattgccg gggtcggcgt atttggcggt aatgacttcg ccttgttcgg
     3901 taatgcggat ttgtcccgcc acgctgcccg ccggttgggc gagaatggct tggtaagaag
     3961 ggccgccgcc gcgtccgacg ctgccgccgc gaccgtggaa caggcgcatg cggacatcgt
     4021 attttttgaa gagttcgacc aagcccaatt ccgcctgata cagacaccat gagctggtaa
     4081 catagccgcc gtctttgttg gagtcggagt agccgagcat aatttcttgg atgttgccgc
     4141 agctttcgag cagggcatcg taccaatcga ggcggaacat ggtttccatg accggacagg
     4201 cgttttcgag tgcttcgatg gtttcaaaca gcggcacgat attgatgcgg ctgcgcggtt
     4261 tgccgttttc caccgccagc aggccgcttt ctttcagcag caatgccaag gcgagcaggt
     4321 cgctgggttg ttcgcagttg gaaataatgc tttgtgtaac ggcatcttcg ccgaattcgt
     4381 ctttgatttt gcgcgcttcg ttgaaaattg ccagttcgtg gcgggtatgg tcgctgtatg
     4441 tgatgaacgg gctgtacagc ggacgttgat ggctcaattc gcgcaacagg gcggcttgtt
     4501 tttgctcttc gttcaggctg ttgtagtctt ccaagcctgc gtgttggaaa agctcggcaa
     4561 ccacatcggc gtgtttgcct gcgtgttggc gcaggtcgag cggcatcatg tggaagccga
     4621 acacggacac ggaacggatg aggtctgcca aacggccttc ggcaagcagg cggctgccgt
     4681 tgtcgataag ggaacgttgc aattttttca aatcatcgag aaattcttgt gccgaagcat
     4741 aaggctcgag aaagccgaat ttgcagccca tgcccaaacc gagcgcgcgc gctttgccca
     4801 tagcgcgcgc cataatgtag gcaatggcgc ggcggtaggg ttcttccgtg cgggcaagtt
     4861 cttcgtcagg cgagagggct gccagtgcca ttacgtcgtc gttgactttg acgcggcgga
     4921 tggagagcgg caattcgcgg tagagtttgt cgagttcgcc acggtaaaaa cggaacacgg
     4981 catcggcgtg gcggcggaag gcaaagcgca gggtttcggc agaaacgaac ggattgccgt
     5041 cgcggtcgcc accgatccag ccgccgattt tgaggatgtt cggaacgcgg acatcgggat
     5101 aggccgtctg aaagtcgtgt tccatcttgc ggtagagttt gggcagggct tcaaaaaagc
     5161 tcatcgggaa gatggacacg ccgttgttga tttcgtcgtt gacgctgagt ttgtggcggc
     5221 gcgtttcgct ggtctgccac aaacccagca ggatagtgtc gatttcgcgg cgcagccgtg
     5281 ccagcgcgtc ggcattggtg cagcgttcgc gttgcggcag cagcgcgcgg atgcggcggt
     5341 tgaaattcaa aacggtttgg cgttgcactt cggtcgggtg cgcggtcaaa acggcggtaa
     5401 cggacgtatt gtccaactgc cgctgcaccg atttgccgtc ggctttcccc gctttgagcc
     5461 tgcggacggt ttccgtcagg ctgccttccg cgccgccgcg tccggcttct tcgtggattt
     5521 ggcggcggcg ttcgtggtgc acgtcttcgg cgatattcaa aatctgggcg aacaggccgc
     5581 aggccaaggt taaatcgtgg gtttgttgtt cgtccaattg cggcaatact ttttcaatca
     5641 atgccgcgct gtcgtcggaa gtggacaaga gtttgaccgt ttcgacaacc aatggcgagg
     5701 cttcttcgtg caggaggttg aacagggatt gtttcagaaa ttccgcgtcc gccgccaaag
     5761 ccgcgtcctt tggattgttc agaatatgca gttgcatgat ttttctctca ttgccgtaaa
     5821 tactgtaaat gtacctcaaa tgccgcatcc gtgccaaacc gttcacactt taaccgcccg
     5881 tgtcccgaaa tgccgtctga agttgaacgc cgcccgacgg cagcgttaca atcgcccgca
     5941 actgtttttt ccgaacatca tcatgaccac gaccgaacac gacaacgacg atgcattcct
     6001 gctgcggtac agccggcaca tcctcttgga cgaaatcggc atcgaagggc agcagaaact
     6061 ttccgacgcg catattttgg tcgtcggatg cggcggtttg ggtgccgccg cactgcccta
     6121 ccttgccgct tcgggtgtcg gcacgctgac catagccgat ttcgacacgg tcgaactgca
     6181 caacctgcaa cgtcaggttg catttgacga gggcgacgtt ggcaaactca aaaccgaagc
     6241 cttggcagac cgtctgcacc atatcaacca taccgtcgat gtccgcaccg tcaacgaaaa
     6301 actcgacagc ggccgcctga ccggtttggt tcaaaccgcc gatatcgttt tagactgctg
     6361 cgacaactat gccacgcgcc aagccgtcaa ccgtgtctgc gtacaaacga aaacaccgct
     6421 ggtttcagga gcagcggtgc gctttgaagg gcaacttgcc gtgtaccgtc ccgacttgcc
     6481 cgactcgccg tgttacgcct gcctgtttga cggcggatcg gcttcagacg gcatctgttc
     6541 cctcttcggc gtgttctcgc cgctggtcgg catcatcggc agtacccaag cggcggaggc
     6601 tctgaaaatc ctgctggatg cgggcgaacc gtcgcacggc aggctggcgg tttaccgtgc
     6661 cttggaaggg ggctggcaat atttcgacct gccgcgcaac cccgaatgcc cgatttgcgg
     6721 cgcggaacgg taaaaccctg ccgccgtttc agacggtatc caaacggatg cggcggaacg
     6781 gttttaaaaa tttaaaaatt tacatttctt tgcaaaaaaa aataaactta ccttataatt
     6841 gcagttgttt tagcaatgtc tgtttcgcag actcattgag taagacgttt tccccgtaat
     6901 gtgtttggcc gtctgtcccc tttgggttcg gacggctttt ttttggctgt gtttgaatac
     6961 ccggttgatt tcgtctgttt gcagggggaa agccgcttat ttccgttcgg gtggaaaacg
     7021 gttccatcgg ataaaaggca ttttgtccga ctgattaaag ttacatctta gagccgcatc
     7081 ggcggaatat tcaaacacag cttttttaag gaaatccgga tacggcggcg catcaataat
     7141 gcggcggaat ctcgtcgcgc agggaatacg gctcttgcgc gtcgggattc ctgtcctgca
     7201 ttttttgata cagcagcctc aactgagcct gctgcaaatc cagcgtctgc cgcaattccg
     7261 ccaccatcgc gttcaggcgg gcgattacgt cctcctgaag cgcggattgg atttccaatt
     7321 cgacaatacg gcgttccaac tcttgaaccg cgtccattta cagcaccatt gcggcaatcc
     7381 agccggcaat cagcagcggg atgttgtagt ggatgaaggt cgggataacg gaatcgcgga
     7441 tgtggtcgtg ctgcccgtcg gcgttcagcc ccatcgtcgg gcccagcgtg gaatcggacg
     7501 caggcgaacc ggcatcgccc aacgcccccg ccgtgccgac aatggcgacg gtggcaagcg
     7561 gcgaaaaacc caaaccgaca cacaaaggca cataaatcgc ggcaataatc ggcaaagtgg
     7621 aaaaggacga accgatgccc atcgttacca aaagccccac caccagcatc gccaatgccg
     7681 ccataccttt gctgttgccg aatatcgcca tactgctttc caccagcggc tgaatatgcc
     7741 cggtcgcatt catcacggcg gcaaaaccct gcgcggcaat cataatgaag ccgaccatcg
     7801 ccatcatctt gataccttcg ccgaatacgt cgtttgcctt gtcgcggtta atgaccccca
     7861 acatcataaa tacggcgaaa ccgagcatcg cgcccaacac cagcgagtct tcatacatca
     7921 actggatggc aaagcatacg gcaatggcga cggcggcggc caggctgcgg taggcggacg
     7981 gctgcggacg gtttgccgca tcggcgttgc ccgccgtatc ggcattgttg ctttggtaca
     8041 ggcgcggttt gcggtaatgg acaaacgcca gcaggagtcc ggccagcatt cccaacgcgg
     8101 gaatcgccat tgccgccatc acgttaatgt ttttcacatc aagctgcggc gcggcggaat
     8161 ggatgttgcc caacaggatt tcgttcaaaa aaatcgcgcc gaagccgtaa ggcaggaaca
     8221 tataagtcgt aaccagcccg aaagtgatga cgcacgcaat caggcggcgg tcgattttca
     8281 ggcggttgaa caccaaaagc agcggcggaa caatcatcgg gataaaggca atgtggatgg
     8341 ggatgatgtt ctgactcata atgcccatca caaggatgat ggaaagcagc agccatttga
     8401 ccgcgccctc gcccgaacgc acgctgtcgg gcataccgcc ccggttcagt ttgcggacga
     8461 ccgcgccggc aagctgctgc ggcaggccgg aatgggtaat cgccatcgca aacgcaccga
     8521 gcatcgcata agaaagcgca atcttcgcgc cgccttccaa acctttgttg aacacgggga
     8581 taatccccgc ctgactgacc tgtcccgccg catcggcaat gttttgcagc ggcatacccg
     8641 ccaccgcgcc gccgacaaac gcgccgaccg tcaggctcaa taccacgtgc acgcgcgaca
     8701 gcgacagcga cagcataacg attacggcaa ctacgactgc attcattgtt tatcgctcca
     8761 aacctataaa tgtttacata tcgaaacaca tcataaccca ataacggaaa acccgccaat
     8821 tttgcaaaca attatttcaa atgcttcata tacttcccca gcgtaaccct gtccaaaccc
     8881 gccaagtccg gcagggtttc cactcccgaa aaaccattct ccgccaacac gccgcgcacc
     8941 gccgcgccct gatcgaaacc gtgttccagc aataaaaaac cgccttccgc caaacggtcg
     9001 ggcgcgcctt gcgccaaggt gcggatgcag cttaggccgt ctgaaaagtc ggtcagcgcg
     9061 atttgcggct caaaccgcaa atcgccttgc aacaaatgtt tatcgccgtt ttcgatatag
     9121 ggcgggttgg acacgatgat gtcccatttc ccttcagacg gcatatcggt gtcgaaccac
     9181 gaaccgtgtg caaattcgac ccgcgcgccc aaatctgccg catttttccg cgccgtttca
     9241 agggcgggcg tgctgatgtc ggatgcgcgc acaaacgcat cggggcgttc gagtgcgacg
     9301 gttacggcaa ccgcgccgct gcccgtcccc aaatcccaca cgcgcccgtt ttcgggcagg
     9361 cgcgccaata cggcttcgac caagtgttcg gtttcggggc gcggaatcag cacgctcgga
     9421 ttgactgtaa agcgtctgcc ataaaattcg cgcacaccta aaatataggc aaccggctcg
     9481 ccgttcagac ggcgttgcgc cagcctgtcc gcccgctgtc ggacttcgtc cggcatttct
     9541 tccccgcccc gcgtcaacaa ctgcacgcgc gtatattccg aaacatattg cagcagcatt
     9601 cttgcttcaa ttttaggcag ttttgacaag cccaaccatt cgtcaaacgt cattttttat
     9661 cccgtctgcc gccgatgcgg cttttctttc cttattcttt ccggcaaacg taccgatggt
     9721 ggcaaccgca aatgcggcat accacaaata aaatcctgca ccgtagcgca caatatccga
     9781 tgtattccct gcttcatcga cgtatacggc tttcacactg aaagccacca acgccaagcc
     9841 ccaaagtgcc gcatggacag gcacgacctt cttccgcaac gccagcaaaa caatggccgc
     9901 caaccaaaca taattcgcat agaccgcaca atacctgata tccaaagaag caaatatcga
     9961 ccccaaaatc aaaacggtca aaccctccat gcttccatga ttgcccaaat aaaatgcaac
    10021 attggataaa gacgctatcc acagggcaac cgacaccagc aacatcacta tgggaaaact
    10081 tgatttccga ttctgttcct gcatggtttt atcctaatgt aaaaggccgc ctgaaaacct
    10141 ttcagacggc atcgtgccgg attccgcgtc agattgcgct gccgccgacg gtcagtccgg
    10201 catcaatccg caaagtcggt tgccccacgc cgacggggac gctctgccct tctttgccgc
    10261 acacgccgac accgctgtcg agcgcggtat cgttgcctat catcgaaacg tgtttcagca
    10321 cttcgggacc gttgccgata atggtcgcgc ctttgacggg atattgcagc ctgccgcctt
    10381 ccacccacca tgcttcggac gcgctgaaca caaatttgcc gctggtaatg tccacctgtc
    10441 cgccgccgaa gttgacggcg taaatgcctt tgtcgatgga cgcgatgatt tcttccggct
    10501 catagctgcc gttttccata aaggtattgg tcatgcgcgg cataggggcg gaggcgtagc
    10561 tttcgcggcg gccgttgccg gtcgattgcg tacccgtcag gcgggcgttg gtttcgtcct
    10621 gcatatagcc ggtcaaaata ccgtcttcaa tcaatacggt gcggcgggtt tcgttgcctt
    10681 cgtcgtcgat gttgagcgaa ccgcgccggc cggcaatatc gccctgatcg acgacggtaa
    10741 cgcctttggc ggcgacgcgc tcgcctattc tgccggaaaa gacgctggtc cccttgcggt
    10801 tgaaatcgcc ttccaaaccg tgtccgaccg cttcgtgcag caacacgccc ggccagccgt
    10861 tgcccaaaac gacggtcatt tcgccggcgg gcgcggggcg ggattcgagg ttggtgagtg
    10921 cctgtttgac ggcggcatcg acaaaccgat gaaccaagtt ttcatcgaaa taagccaagt
    10981 cgtagcgtcc gccgccgccc gcgctgccct gttcgcggcg ttcgccctgt ttggcgataa
    11041 cggtaacgtt caggcgcacc atcgggcgga tgtcggcggc gtgtttgccg tccagacggg
    11101 cgaggtaaac catatcgtat tcgcaggtca aaccggccat cacttgcacg atgcgcggat
    11161 cggcggcttt ggcgattgct tccactttgt tcaacagcgc gactttggcg gcggaatcga
    11221 ggccggcaat ggggtcggac gcggaacaaa ccggcttgcc gcgcgtttca gacggcattt
    11281 tggcggacac cttgccgcct gccgccccaa tcgcgcggac ggcgcgggcg gaacggttta
    11341 tcgaatcgat gcacaggctg tcggcgtagg caaaggcggt tttgtcgccc gaaacggcgc
    11401 gcacgcccac gccctgattg atttggaagc tgcccgattt gacgatgccc tcttccaaat
    11461 gccagctttc ataagcggtg cgctggcagt agatgtcggc gtaatcgacg tggtgcgcgc
    11521 cgatgatgca caggcttttg gcgagcagtt cgggggaaag gcggttggct tcgagcagcc
    11581 gcgcctgtac ggcggaatag gtcggatgca tagtgtcggc gcataaaaaa tcaggggctt
    11641 gattatacgg catttgttat ggaaatgcgc tgtgccgccg aaaatgtaag aattttgcca
    11701 acgcacgttg ctttgtgtaa acttaaagct ttcttgtcgg agtgccgccg ccgggcggct
    11761 gagattgcga aagcagaatc cgtagaacct gtcggggtaa tgcctgcgta ggaaacaaac
    11821 cgtcaaatgc cttatcaggc ttccgttccc ttttccgcac ttccccgccc accttccttc
    11881 tttttaaagg acgtcatatg tcgggcaatg cctcctctcc ttcatcttcc gccgccatcg
    11941 ggctgatttg gttcggcgcg gcggtatcga ttgccgaaat cagcacgggt acactgcttg
    12001 cgcctttggg ctggcagcgc ggtctggcgg ctctgctttt gggtcatgcc gtcggcggcg
    12061 cgctgttttt tgcggcggcg tatatcggcg cactgaccgg acgcagctcg atggaaagcg
    12121 tgcgcctgtc gttcggcaaa cgcggttcag tgctgttttc cgtggcgaat atgctgcaac
    12181 tggccggctg gacggcggtg atgatttacg ccggcgcaac ggtcagctcc gctttgggca
    12241 aagtgttgtg ggacggcgaa tcttttgtct ggtgggcatt ggcaaacggc gcgctgattg
    12301 tgctgtggct ggttttcggc gcacgcaaaa caggcgggct gaaaaccgtt tcgatgctgc
    12361 tgatgctgtt ggcggttctg tggctgagtg ccgaagtctt ttccacggca ggcagcaccg
    12421 ccgcacaggt ttcagacggc atgagcttcg gaacggcagt cgagctgtcc gccgtgatgc
    12481 cgctttcctg gctgccgctt gccgccgact acacgcgcca cgcgcgccgc ccgtttgcgg
    12541 caaccctgac ggcaacgctc gcctacacgc tgaccggctg ctggatgtat gccttgggtt
    12601 tggcagcggc gttgttcacc ggagaaaccg acgtggcaaa aatcctgctg ggcgcaggtt
    12661 tgggtgcggc aggcattttg gcggtcgtcc tctccaccgt taccacaacg tttctcgatg
    12721 cctattccgc cggcgcgagt gcgaacaata tttccgcgcg ttttgcggaa acacccgtcg
    12781 ctgtcggcgt taccctgatc ggcacggtac ttgccgtcat gctgcccgtt accgaatatg
    12841 aaaacttcct gctgcttatc ggctcggtat ttgcgccgat ggcggcggtt ttgattgccg
    12901 actttttcgt cttgaaacgg cgtgaggaga ttgaaggctt tgactttgcc ggactggttc
    12961 tgtggcttgc gggcttcatc ctctaccgct tcctgctctc gtccggctgg gaaagcagca
    13021 tcggtctgac cgcccccgta atgtctgccg ttgccattgc caccgtatcg gtacgccttt
    13081 tctttaaaaa aacccaatct ttacaaagga acccgtcatg acccgtatcg ccatcctcgg
    13141 cggcggcctc tcgggaaggc tgaccgcgtt gcagcttgca gaacaaggtt atcagattgc
    13201 acttttcgat aaaggctgcc gccggggcga acacgccgcc gcctatgttg ccgccgccat
    13261 gctcgcgcct gcggcggaag cggtcgaagc cacgcccgaa gtggtcaggc tgggcaggca
    13321 gagcatcccg ctttggcgcg gcatccgatg ccgtctgaac acgcacacga tgatgcagga
    13381 aaacggcagc ctgattgtgt ggcacgggca ggacaagcca ttatccagcg agttcgtccg
    13441 ccatctcaaa cgcggcggcg tagcggatga cgaaatcgtc cgttggcgcg ccgacgacat
    13501 cgccgaacgc gaaccgcaac tcggcggacg tttttcagac ggcatctacc tgccgaccga
    13561 aggccagctc gacgggcggc aaatattgtc tgcacttgcc gacgctttgg acgaactgaa
    13621 cgtcccttgc cattgggaac acgaatgtgc ccccgaagac ctgcaagccc aatacgactg
    13681 gctgatcgac tgccgcggct acggcgcaaa aaccgcttgg aaccaacctc ccgaatacac
    13741 cagcaccttg cgtggcatac gcggcgaagt ggcgcgggtt tacacacccg aaatcacgct
    13801 caaccgcccc gtgcgcctgc tgcacccgcg ttatccgctc tacatcgccc cgaaagaaaa
    13861 ccacgtcttc gtcatcggcg cgacccaaat cgaaagcgaa agccaagccc ccgccagcgt
    13921 gcgttcaggg ttggaactct tgtccgcact ctatgccgtc caccccgcct tcggcgaagc
    13981 cgacatcctc gaaatcgcca ccggcctgcg ccccacgctc aaccaccaca accccgaaat
    14041 ccgctacagc cgcaaacgcc gcctcatcga aatcaacggc cttttccgcc acggtttcat
    14101 gatctccccc gccgtaaccg ccgccgccgt cagattggca gtggcactgt ttgacggaaa
    14161 agacgcgccc gaacgtgatg aagaaagcgg tttggcgtat atccgaagac aagattaaag
    14221 ccgtctgaaa ggaaccccat gaccttcccg ccactcaaat ccccgctcaa attctacgcc
    14281 gtcgtcccca ccgccgattg ggtggggcgc atggtcaaag caggtgccga cacggtgcaa
    14341 ctgcgctgca aggccctgca cggcgatgaa ttgaaacgcg aaatcgccct ctgtgtcgcc
    14401 gcctgtcagg gcagccatac gcagcttttc atcaatgacc actggcgcga ggcaatcgaa
    14461 gcaggcgcgt acggcgtgca tctcggacaa gaagacatgg acaccgccga cctcgccgcc
    14521 attgccgctg ccggtttgcg cttgggtttg agtacgcact ccgtcgccga actcgaccgc
    14581 gccctgtccg tgcaccccag ctacgtcgcc agcggcgcga ttttcccgac cacgaccaaa
    14641 caaatgccca ccgcccctca aggcttggaa aaactgcgtg aatatgtgaa acaggcaggc
    14701 agcacgcccg tcgtcgccat tggcggcatc gatttgaaca acgcccgagc cgtactcgcc
    14761 accggcgttt cctcactcgc cgccgtccgc gccgtaaccg aagcggcaaa tcccgaagcg
    14821 gtggttaaag cgtttcaggc tttgtgggat ggataaaacc gaaagaagaa aattcaattg
    14881 ccgtgtaggc aaaacttagc ccgttatcgc aaacatactt aactttaaat gtggcatatc
    14941 atcaaattcc gtcatttccg cgcaagcggg aatccgcctt aaaacttgag aaaccatcat
    15001 ttgaaaaaca gtttccgaat ttcaaaaatg gattcccgcc tgcgcgggaa tgacggcaac
    15061 cggccggttg cgtatcaaaa aataaagtaa ttcggctaga tatagtggat taacaaaaat
    15121 caggacaagg cgacgaag
//
LOCUS       DAWKQJ010000035        15072 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000035 DAWKQJ010000000
VERSION     DAWKQJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.035"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(47..367)
                     /locus_tag="WJL20_000819"
     CDS             complement(47..367)
                     /locus_tag="WJL20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HEZ7682991.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
     gene            563..1021
                     /locus_tag="WJL20_000820"
                     /pseudo
     CDS             563..1021
                     /locus_tag="WJL20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            921..1289
                     /locus_tag="WJL20_000821"
     CDS             921..1289
                     /locus_tag="WJL20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ7682992.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDNLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLVGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1358..2896
                     /gene="lnt"
                     /locus_tag="WJL20_000822"
     CDS             1358..2896
                     /gene="lnt"
                     /locus_tag="WJL20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ7682993.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFSLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3160..4023
                     /gene="rpoH"
                     /locus_tag="WJL20_000823"
     CDS             3160..4023
                     /gene="rpoH"
                     /locus_tag="WJL20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ7682994.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            4507..5391
                     /locus_tag="WJL20_000824"
     CDS             4507..5391
                     /locus_tag="WJL20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ7682995.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGSIDNDRLQQEFALFVQKS
                     DVDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5475..5990)
                     /locus_tag="WJL20_000825"
     CDS             complement(5475..5990)
                     /locus_tag="WJL20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7682996.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6066..6485)
                     /locus_tag="WJL20_000826"
     CDS             complement(6066..6485)
                     /locus_tag="WJL20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682997.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6606..7604)
                     /locus_tag="WJL20_000827"
     CDS             complement(6606..7604)
                     /locus_tag="WJL20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ7682998.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7606..8085)
                     /locus_tag="WJL20_000828"
     CDS             complement(7606..8085)
                     /locus_tag="WJL20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7682999.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8137..8916)
                     /gene="pgeF"
                     /locus_tag="WJL20_000829"
     CDS             complement(8137..8916)
                     /gene="pgeF"
                     /locus_tag="WJL20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ7683000.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9474..10421
                     /locus_tag="WJL20_000830"
     CDS             9474..10421
                     /locus_tag="WJL20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ7683001.1"
                     /translation="MNILSKISSLIGKTFSLWAALFAATAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10574..11698)
                     /gene="rluD"
                     /locus_tag="WJL20_000831"
     CDS             complement(10574..11698)
                     /gene="rluD"
                     /locus_tag="WJL20_000831"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ7683002.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTLWIKEGAVIVNDKPSQPKDKMIGGEQIRVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWSGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NLRHPCGDTVKEAIKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11697..12500
                     /locus_tag="WJL20_000832"
     CDS             11697..12500
                     /locus_tag="WJL20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ7683003.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12558..14777)
                     /locus_tag="WJL20_000833"
     CDS             complement(12558..14777)
                     /locus_tag="WJL20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ7683004.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALHP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRIRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3607 a         4543 c         3694 g         3228 t
ORIGIN      
        1 gtactgtctg cggctcgccg ccttgtcctg atttaaattt aattcactat atcgaccttg
       61 ccgtcgggca ggatgtacca cgccaaatcc ccgcctgaag cacggtgtcg gactgcattt
      121 cgattcggct atacgccttt tcacgcaggc tgtaattgaa tttccacaaa ccgccgaaca
      181 ctgccggcgg acgcgcgacc agcatcagga acgccgcaat cgccaaggct tccgccgccc
      241 cgtccaacac gccggcggga gaaaccagca gcaaaggcat tcccaaacgt gcgggtaaag
      301 tggtcgaaac cacgctcaga aacaacagtg cgccacccgg caggaacaga ctgcccatcc
      361 aatccacgct aacgattctc ccttaaaatt catgaaatta aaatcgaaag actgtaacac
      421 ggcattttct tttcacgaaa ctttacttca cgtccgccac agcgttcaga cggcattttc
      481 ctgtttcatg ccgcctgaaa ccgcccaaat ccgcgttctc ctatataatg tcggactttg
      541 cgccgcattt caggattctt taatggcatt tgcctcacta tttaccctgc cggacgacat
      601 caccgccgtt ttggacgatg ttgccctgat gaccaaagcc gccgcaaaga aaaccgccgg
      661 cgtggtcggc gacgatttgg cacccaatgc caaccgggtt acgggcgtat cggcagaacg
      721 cgagttgccg attgtttggt cgatggcgaa aggttctttt tccaacaagc cggttttggt
      781 gcctgcggcc ctgctgcttt ccgccttcct gccgcaactc attacgccgc ttttgacggc
      841 aggcggcatt tatctgtgct ttgacggcgt agaaaagctg ctgcacaagt ttctgcaccg
      901 acatgacgcg catgctcgta atggcggcaa tcggcatcgg catgactgtt ttggtgtacg
      961 gaatcgttgc cgtcatcgtc aaactcgaca acttaggcat gctgctgatg cgccgcccac
     1021 aaaccttttc gcgcagtttg gggcaaatgc tgactgcctt tatgccttgc tttatgcgcg
     1081 gtttgagcgt ggtcggcacg cttgccatgt ttcttgtcgg cggcgtactg gtcgcccaca
     1141 acctcggcct tctgcacgat ttcctgcacg cgcaacactg ggacgctggc tgggcggagt
     1201 acttcgccaa cctcgttgtc gggctgcttt ccggttcgat tgcctgcgcc cccgccttgc
     1261 cgctgatgaa tcgtttcggc aggcattgat ttctttttca aataccgatg ccgtttgaaa
     1321 gatgttcaga cggtatcttc cgaacagaca gatgaatatg ttttccaaac tggacaaata
     1381 ctggcagcac cccgccctct actggccttt gctcatcctt tttgccgccg ccaccccctt
     1441 taccttcgca ccctactacc acttttggct gatgcccttg attttcggtg ccttcgtccg
     1501 cctcatcgaa ctgcgtccgc gttttgctgt ctcttccgcc tacctgttca gcctgaccgc
     1561 atacacgaca cagttctact ggatacacac cgccctgcac gacgtttccg gcctgcccga
     1621 cctctatgcc gtaccgctga ctttcctact ccccgcctac cttgcccttt atccggcact
     1681 gtgtttctgg ctgtggaaaa aatttaccct gcctcggggc ataaaaatcg gtttggtact
     1741 gcccatcctg tggacactga ccgagtttgc ccgcgaacgt ttcctgaccg gattcggctg
     1801 gggcgcaatc ggctactccc aaatcacccc ggacagcccg ctcgccggct ttgccccatt
     1861 gggcggcatc cacatggtta cactggcaac cgcctttctc ggtgtctggc tggttttggc
     1921 gagtgataac accgcacgtt cgggcaaacg cctgcttcca attatcctga ttgccgccct
     1981 gcttgccgca ggctacaccg cccgacaaac cgacttcacc cgccccgacg gcagccgcag
     2041 caccgtcgcc ctgcttcaag gcaacatcga ccaaaccctc aaatggcgtg aagaccaagt
     2101 catcccgacc atacagaaat attacgaaca ggtcggcaaa accactgccg acatcgtcat
     2161 cctgcccgaa accgccatcc ccgttatgcg ccaaaacctg ccggaaaaca tactggcaaa
     2221 atttgccgaa caggcgcaaa acaacggcag cgcgctcgcc gtcggcatca gccaatacac
     2281 ttcggacggc aacggttacg aaaacgccgt catcaacctg accggttatc aggaaaacaa
     2341 tcaggacggc atcccctact acgccaaaaa ccacctcgtc cccttcggcg aatacaaacc
     2401 gctgcccttc ctgaccacgc cgctttacaa aatgatgaat atgccccttt ccgacttccg
     2461 caaaggcggc ggtaagcaat ccgccctgct gatgaaaaac caaaaaatcg ccttcaacat
     2521 ctgttacgaa gacggattcg gcgacgaact gattgccgcc gccaaagatg ccaccctgct
     2581 tgccaatgcc agcaatatgg cgtggtacgg aaaatccaac gccatgtacc agcacctcca
     2641 acaatcgcag gcacgggcta tggaactcgg acgctatatg gtccgtgcca ccaacaccgg
     2701 cgcaaccgcc atcatctccc ccaaaggcaa catcatcgcc caagcccaac ccgatacgga
     2761 aaccgtattg gaaggacaca tcaaaggcta tgtcggcgaa acaccctata tgaaaaccgg
     2821 cagttcatgg tggttgatgg gcatattgac cctagccgca ctgattcttt tcatcttccg
     2881 aaacaaagaa cactgatacc tgcacagccc gtccggacgg gctgtctttt ttcaaaccgg
     2941 ctgtttacat acaactacgt atgtaatata attccgaaat cggattgagc catcaggttt
     3001 acaaaatcaa tcaaacctgc cttacaaaat tttcctgtct ttcaaacctt agcgcatatc
     3061 tcctgccaaa catacaaaat acaagccaag cagtgatata ctgcatccca acacagcaaa
     3121 ccgccgatac gcagtttcag ccgaaaggaa atgccccaaa tgaataacgc tttcgcatta
     3181 cccgccatcc aaagcggcaa cggcagcctc gaacaataca tccacaccgt caacagcatc
     3241 ccgatgctgt cccaggaaga agaaacccgc ctcgccgaac gccggataaa gggcgacctc
     3301 aacgccgcca aacaactcat cctgtcccac ctgcgcgtcg tcgtttccat cgcgcgcggc
     3361 tatgacggct acgggctgaa tcaggcagac ctgattcagg aaggcaatat cggactgatg
     3421 aaggcggtca aacgctacga gcccggtaga ggtgcacgcc tgttttcatt tgccgtgcat
     3481 tggattaaag cggaaattca cgaattcatc ctgaggaact ggcgccttgt ccgcgttgcc
     3541 accaccaaac cgcaacgcaa gctgtttttc aatttacgca gtatgcgtaa aaacctcaac
     3601 gccctgtctc ccaaagaggc acaagacatc gccgacgatt tgggtgtcaa actttccgaa
     3661 gttctggaaa tggaacagcg catgacggga cacgacatcg ccatcatggc agacaacagc
     3721 gacgacgagg acagtttcgc ccccatcgac tggcttgccg accacgattc cgaaccgagc
     3781 cgacaactat cgaaacaggc gcattacgcc ctgcaaacag aaggtttaca aaacgctttg
     3841 gcgcaactgg atgacaggag ccgccgtatt gtggaaagcc gctggcttca agacgacggc
     3901 gggctgacgc tgcaccagct tgccgccgaa tacggcgtat ctgccgaacg catccgccaa
     3961 atcgaagcaa aagccatgca gaaactgcgt ggtttcctga ccgaagaagc cgaagcggtt
     4021 tgaaatatac gcaataaaat acccgacaaa ttgttttgtc gggtatttta tttggtaaac
     4081 atcaatgcgg tttgtgcgtc gtgccgtaca cgattttatc cgtccaaacc atcgcaatcg
     4141 ccgaaatcaa aaagcagaca tgcaccagac actgccgcat cagctgcttt tccgacagat
     4201 tggcggaatt gatatagtgg attaacaaaa atcaggacaa ggcggcgaag ccgcagacag
     4261 tacaaatcgt acggaaccga ttcacttggt gcttcaacac cttagagaat cgttctcttt
     4321 gagctaaggc gaggcaacgc cgtactggtt ttggttaatc cactatacat ttcccttcct
     4381 tgcagcaaca aaagcgcgtg attccaatct gccgctgcaa aatcttcaat ttttcacacc
     4441 cgaaaacgct tgccgccata cagatgtgat atattgattc cattttaacg ggcttcggag
     4501 aacgacatga gcagcggaaa tatccacatc cacagcatga ccggctttgc caacgcggcg
     4561 gcggaatgcg gcagcaaacg catcaacctc gacatccgcg ccgtcaacca ccgctttttg
     4621 gacattcaaa tcaggatgcc ggatgatttg cgccatttgg aaagcggtat ccgcgagaaa
     4681 atttcatccc atctggcacg cggcaaagtc gaatgcaaaa tccaaattca agatacggaa
     4741 aacggcaatc agtctttgga actgaatcgc gatctggtcg gacagcttgc cgaaatcaac
     4801 aaagacctcc gcaagcatca cgatttggca aaactgggcg ttgccgacat cctgcgtttc
     4861 cccggcgtat tggcaagcca aagggaaaat acggacgcat tggcaaaggt tattgccgat
     4921 ttgaccgatt cggcactgaa agactttacc gccgcacgcc gacgcgaagg gaaaaaattg
     4981 ggcgaacacc tgctgcaacg cctcgagtct atggaagaaa tcatcggggc attgagcgaa
     5041 atcttccccg cgctagtgga agcgcataaa gaaaaaatcc gcgcccgcct tgccgaagcc
     5101 gtcggcagca tcgacaacga ccgcctgcaa caagagtttg ccctgtttgt ccaaaaatcc
     5161 gatgtcgatg aagaattcag ccgcctgcgc acccacattg ccgaagtgcg ccgtatcgtt
     5221 accgaacaca aaggcagcag cggaaaacgg ctggacttcc tgatgcagga attaaaccgc
     5281 gaagccaaca ctttgggcag caaatccatt gccgccgaat gcacccaagc ctcggtcgag
     5341 ctgaaagtcc tgatcgaaca gatgcgcgaa caggtgcaga atatcgaata atcggtttcc
     5401 ccgataagca aatgccgtct gaaagccctt tcagacggca ttttttcagg ttgaaacctt
     5461 tgcggtataa aggcttaagg ctttcctttg accgccacca atcttgttcc tgcaagaaaa
     5521 tcatacagaa actgccgatc gggattcagc agtgcgaacc cccacggtaa aatcagccaa
     5581 atcaatgccg caccgaccgc gcccttgggc ggaatgccga ggaagtggcg caatccggca
     5641 taagcaagca taggaatgaa gacgatgaat atgcacgccc aaataaagcg caggcgcagc
     5701 aggtgcaaag acggctgtat gccgttaagg tcgcacaagc cgattttcca tgtcctcatc
     5761 gccaaggtct gcccctgacc gtgccagttg gcgcgaaaat aaagccacca tgatcccatt
     5821 atcaggatac tcgttaccaa tgcagaaacc gcgatagaaa cgggattcag aaaaatggcg
     5881 gcaataccgg caaacaatgc tgccaaacag gttgccgcac cgaccagcag catttcgtac
     5941 agcatggcgg caaagcggcg tttgagcggg gcggttttca gtgtcatcat cttttcaatc
     6001 agaatatgcc gtctgaaacg ccaacaggct tcagacggca tttatgcaaa gatgccttgc
     6061 ggcggttaac ggtgcggacg tttctgaccg cgcgactgtt ttgccgccat ttgattgatt
     6121 tcctgacgtg tcgggcgttt tttgaacata gcgtgcgcct cgtccaccga acatttttgc
     6181 gacaggcaga tcagggtaat cgcatctttc tggtgttgtc cgccgctgtg gtacatacgg
     6241 cgcaggtttg cctcgttgat tctgcctttt tcgggatcga tgtcgaattt tttcaggcgt
     6301 tcgataaact ggcgttgctg tcggcgggat gtccaaacaa aaccgcccgc agcggcggcg
     6361 acgatgacgg cggcaagcag gatggcaagc ggccaggagt cgacaatcaa tacgataatg
     6421 aggcttaaaa cggcggtggt cagtaaaatc aggccgccca agcgcagttt ggtcttattg
     6481 tccatagggt taatgttcct ttattgaggt ggtcgtgccg tctgaaatgt tttcggacgg
     6541 attggtgaat acttttcagg attaacggta ttttacagta ttttcggatt ttggggatta
     6601 ccgctttatt ccgccagcga cacgacaagc cgtttgaata ccgtccatgc gtcgccgtct
     6661 tccgcacctt tgatgattcg gtcgatttgg gcgcaggttt tgagcgcgtc aagcaggcgg
     6721 acgacggaaa tccgcttgac tgcaagcggt gcgagcgtct gcttttcacc ccaaagtctg
     6781 agactgttgc gtacggattg gacactctgc ccctgcttca gggcggcagc aagccggatc
     6841 agcgtccgca cgtcttcggc aaccgcccac agcaacagca ccggttcttc gccctcttcc
     6901 cgcaatccgt ccaaaagcct gcatacgcgc aggacatcgc ccttcatcca cgcgcctgcc
     6961 agttggaacg cgtcgaagcg ggcgacgttg gcaacggcgg tttgcgcctc atcgatattg
     7021 acggtatgcc ctttcggata cagcagcccg agcttgtcga tttcctgacg cgccgccaac
     7081 agattgcctt ccacgcgctc ggcaaacagt gccaatgcgt ctgccccgat acccaaaccg
     7141 attttgtcca gccgtccgcg tatccattgg ggcaaagccg ccgcgccgac cggtttggct
     7201 tcccacactt ccccctttgc cgccaatgcg gcaaaccatt tggactggag ctgggttttc
     7261 tccagtttgg gcagcaaaac cagcgttacc gtatcttccg gcaggcgggc ggcaaaatcc
     7321 tgcaacgcct cgccgccggt tttgccgggt tttccgttgg ggatatgcag ttccaacagc
     7381 ttcaaatcgg caaacagacc cgcactgcct gcggtttgca acagttcgtt ccaatcgaag
     7441 gcattgtctg ccgtataaac ttcccgatta aggtaacctt gtttcttcgc cgccgccctc
     7501 aatgcgtcca atgcctcgat acgcaacagt tcttcctcgc cgtggatgac gtacaggggt
     7561 ttcaaaggcg cgtccgtatc aatgtgtccg atatgtgccg ccacgtcatt ccgccttcag
     7621 aaaggtcagg cggcggacaa tctgttcggc ggcatcctgc cgcatttccg cccacaatgc
     7681 cgcttcctct tcctgtttgc ccaagatctc gttgtcggca taagcaagga cgcggcggac
     7741 ggacacggtc atcggtttgc cgaccggctc gccgcgtttc aatacctgcg cttcaaccgt
     7801 caagattaaa agatattcgt tgataactgc cgcacgggta acggtgtagg tttccttgtt
     7861 ttgggaaacg ctgtctatac gcagggtcat ctgcgcgccg gcagcatcgt ccaccctgcc
     7921 cgaagcctga tacagcgcgg tttccaaagg aaactgcaat gcctgtccgc cttcgatgtg
     7981 ccagctccgg taggtcagcg gcggagaaat gccgtctgca cctttcaggt ggaaaccgca
     8041 cgcgcccagc atcagcactg cggcagtaag aaacagtttg ttcatggctt gcctttctca
     8101 aaagcacaaa gtcggtagat tatatcagcg gcgtgttcag acggcattgc cgtccagcca
     8161 aatcaggctc gccatacgcc ctgtcgctcc gtcgcggcgg taggaaaaga aagtatcccg
     8221 ttccaaaacc gtacaatgcg tgccgccata tacgccgccc acgccttcgc gcttcagaat
     8281 caggcgggcg agcgcgtaaa ggtcggcaag gaatttgccg ccgcctatgc cttcaaatgc
     8341 ggcggcggct tcgggcatgg gcgtgcagaa cgcatcaaac acatcctgtc cgacttcaaa
     8401 cgcatccgca ctgatggcgg gaccgagata cgccatcatt tcgacgggtg gaaccttcat
     8461 cgcggcaatg gtgttttgca atacgccgcc cgccaaaccg cgccagcccg cgtgtgcggc
     8521 ggcaaccgcc gtacccgccc tgtcgcaaaa tagaacgggc agacagtcgg cagtcatgac
     8581 agcacaggca actttgcccg tgtcgtctac ggaagcgtcc gcatcgggtg tgcctcccaa
     8641 agcttcggca gcattgacga cgacggtact gtggatttga ttgaggtagg caacgggcaa
     8701 tccgacctgc tgttgcacga tttcgcggtt gcggcgcacg gcttcgggat tgtcgccgac
     8761 gtgcgtaccg aggttcaaac tctgatacgc accttggctc acgccgccgt tgcgcgtggt
     8821 aatcagggtt ttcacattgg cgggcgcggg ccaatcggcg gtcaggaagt ttttgccttt
     8881 cggggcaaga tttagggttt ctgtgatggt tttcattgga tattggaata ggtgtgtggc
     8941 ggaaagggtt gattaactgc cttcggggca aatgaatgct tgttttttcg tcattcccgc
     9001 acaggcggga attcggagat tcgggattgt tttcaaacgt tcgggtttgg attcttgccg
     9061 gtgcaggaat gatgtcctta tataccttct ccgagcttta tatagtggat taaatttaaa
     9121 ccagtacagc gttgcctcgc cttgccgtac tatttgtact gtctacggct tcgtcgcctt
     9181 gtcctgattt ttgttaatcc actatatgtt tcaacagggg atggcacatc aagcaccgca
     9241 ctgcgtgttg cccgaacgag actaccctcc gattagattc tatcgctata aacagatggg
     9301 tttcaaccga aaaggaatgg ggataaagtc catttccgac atctctcggg cgatgccgtc
     9361 tgaaaaccaa tctccacttt cagacggcat tgtttaggcg cagtcatttt gcaaattaag
     9421 gttaaggcgt gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata
     9481 tcctcagtaa aatcagcagc cttatcggaa aaacattttc cctctgggcc gcgctctttg
     9541 ccgccaccgc ttttttcgcg cccgacacct tcaaatgggc cgggccttat attccttggc
     9601 tgttgggcat tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt
     9661 tgttcaaaca tcccaaagtc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg
     9721 caaccgcctg gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga
     9781 ttttggtcgg ctgctgcccg ggcggtacgg cttccaatgt gatgacctat ctggcgcgcg
     9841 gcaatgtggc tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga
     9901 ctcccgccat cttcctgatg cttgccggcg aaatgctgga aatccaagcg ggcagtatgt
     9961 tgatgtccat cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg
    10021 ttttgggcag taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca
    10081 tcgtgctgat tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc
    10141 tgctgatttt tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg
    10201 ccgccaaatg gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta
    10261 tgcaaaactc gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg
    10321 ttgccgttcc gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa
    10381 cttattgggc ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc
    10441 cgtctgaacg gggcaaacga aaccggcggt ttccgcccga ccccttcaga cggcctttgt
    10501 ttgaaatcgg cgggaacgat tgcccggctg ctccggttcg gctgcctgcg ttcgccccgc
    10561 ctttcaagcc gcctcactcc cttacataaa ccacttcgac atcgtagtcg tcttcgttcc
    10621 aatcgtcatc gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg ctcaaagacg
    10681 aatccaaacc ggcttcaaga cggaggacag acaacaaatg atatatgtcg tccggaatcg
    10741 gtgcctcaaa cgaaacggtt tcgccgcttt ccggatgagt gaaacttaag cggtaggcgt
    10801 gcaatgcctg acgcgcgccc aaacttttaa tggcttcttt caccgtgtcg ccgcacggat
    10861 ggcgcaggtt gccgtaaact gggtcgccgg caagcggatg gttggcctcg cgcatatgta
    10921 cgcggatttg gtgcgtcctg cctgtctcga gcgagcattc gatatagctg tgggcaagat
    10981 agcgttccaa cactttgacg tgggtaacgg ctggtttgcc gccgaatttg acgactgcca
    11041 ttttcaggcg gttgtgcgga tcgcgtccga tttgggtttc aattttaccg tcaaagggaa
    11101 cgatgccgtt ggcgacggcg cggtagatgc gtttgaccgt gcgttcttga agctgttgta
    11161 cgagggaatt ttgcgccggc agggttttgg cgaccaccat cagcccgctg gtttctttgt
    11221 ccaaacggtg tacgatgcct gcacgcggta cttggctcaa ttcgggacag tgcgccaaca
    11281 ggccgttgag cagggtgccg ctccagttgc ctgcggccgg atgaacgacc agtccggccg
    11341 gtttgttgac gacaatgacg gtatcgtctt cgtaaacaat atccaaagcc ataggctctg
    11401 gaacaaacgc cagattttcc tcactcggac ggacggttac acggatttgc tcaccgccta
    11461 tcattttgtc tttgggttgc gaaggtttat cgtttacaat aaccgcgccc tctttaatcc
    11521 ataatgtcag gcggctgcgc gaatagtcgg gcagaagttt cgccaacacc gcatccaacc
    11581 gcccgccggc aagctcgagc ggaacggtca gaccgatacg gttttcagtt tcggaagctg
    11641 acgcaaagtc tgaatcgtcg ctataatcgg attcattatc aaaggaagta ttctgcatga
    11701 aaaaaattct tttaacggtt tcattaggtt tggcactgag tgcctgtgcc actcaaggta
    11761 cggtcgataa agatgcccag attacccaag attggagtgt ggagaagctc tatgccgaag
    11821 cccaggacga attgaacagc agcaattata cgcgggctgt caagttatac gaaatcttgg
    11881 aatcgcgctt ccccaccagc cgccatgccc ggcagtcgca actggatacc gcatacgcct
    11941 attataaaga cgatgaaaaa gacaaagctc tggcggcaat cgaacgcttc cgccgccttc
    12001 acccgcaaca cccgaatatg gattacgcgc tatacctgcg cggtttggta ctgttcaacg
    12061 aagatcagtc tttcttgaac aagctggcct cgcaagactg gtccgaccgc gacccgaaag
    12121 ccaaccgcga agcgtaccag gcgtttgcgg aactcgtcca acgcttcccc aacagcaaat
    12181 acgccgccga tgcgaccgca cgtatggtca aactggtcga tgcactgggc ggcaatgaaa
    12241 tgtcggtggc gcgctactac atgaaacgcg gtgcatatat cgccgccgcc aaccgcgccc
    12301 aaaaaatcat cggcagctac cagaatacgc gttatgtcga agaatcgctc gccatattgg
    12361 aacttgccta ccaaaaactc ggcaaaccac agcttgccgc cgatacgcgc cgcgtgttgg
    12421 aaaccaactt cccgaaaagc ccgtttttga cgcacgcttg gcagcccgac gatatgccct
    12481 ggtggcgtta ctggcattaa gcccatcttc gtaatgccgt ctgaagccgt ttcagacggc
    12541 attttatgtt tcaaacctca ttcaaacggt tttttctgcc aatagaattt ataccctctc
    12601 aaacgttgag ccttcacgcc gccgcgcccc aagccgaatt gcagcgcgcc cgacaaatcg
    12661 gtacgcaaca atttgatgcc gtgtgcacgg atacggtttt gcaccgcttc ggtcggatgt
    12721 ttgtaggcgt tggcataacc gcttgaagca acggcatatt cgggcgagac ggcattgagg
    12781 aacacgcccg acgaggacgt attgctgccg tgatgtccca aaaccaaaac ctggctgtac
    12841 aggttgcctc catacttgcc gaccaggctt tcctcgccct tcgtatccaa atcgcccgtt
    12901 accagcagtg ccgcaccgcc cgccacaaca cgcaaaacac aacttttccc attatcatcg
    12961 atgtttttgc gttcagacgg catcaaaaac tcaaaatcca cgccgtccca ttgccaacgc
    13021 tgttccgcac aatgccgcgc cccctcataa aattccggct gcccggcata aatcccgccg
    13081 ttcggtatct tgccgactgc ctgaaaaccg ccgtcgtggt cgctgtcgtg atgcgacaga
    13141 accagcttgt ccaaacggcg gacacccgcc gcattcaaac tcggcacaat ccccatctgc
    13201 gccgccgctg ccgttccggt gtcaaacaaa agatgatgat ttgccgtctg aaccgacacc
    13261 gacaaaccct gccccgcatc ccaaaccgta accgcaacct cattttccgg cacgccgggt
    13321 gaacggtaaa acacaaaccc tgccaacagc aacaccgccc acggacgcaa acccaagccg
    13381 cgcggcagca gcaacagcaa agcggcacac accgccaaca ccaacagcgg caaaggtgcg
    13441 gaggcaacgg caaactcggg cgacacatcg gcaagccaca ccaaaaaccg caaagtatat
    13501 tccgccaaaa atgcccccaa ctgttgcaaa ggcgcaaacg gcacgaccga acccagcaac
    13561 gccagcggcg tcaataccca agaaaaccac ggaatcgcca ccgcattgac caaagggctg
    13621 attaaaggca gcgaagcaaa cagataaccg agcaaaacca acgacaacac cgaagccgcc
    13681 cactgcccgc gcaaagcagt ttgccgtttt ccctcgtgca aacgccccga gcacgcccat
    13741 atcagggccg ccaccaaacc gaaagacagc caagtcccca cacccaagac cgccgacggg
    13801 tcgaacagca gcaccgccgc caacgcctgc caccacgtcg cccacgccga caatcttccc
    13861 ctgcgccaag cccacgcaaa cgccgccaac atcaaaacgc tgcgctgcgt cggcacggaa
    13921 aaaccggcaa gcagcgcgta aaacagcgcg cctgcacacc ccgccgccaa aacccacgcg
    13981 cgcggccggg caggaaggcg cggggaacag gcaagcagcc gcttcgccag ccacgcaaac
    14041 attaccgcca ccatcgtaac gtgcaaaccc gaaatactga ccaaatgcgt cagccccaac
    14101 ggtcggaacg cctgccacaa ttcggggtgc aatgccgact gttcgcccac gctcaacgcg
    14161 cgcatcagcc cgatgccgtc tgaaagcccg ccgtccgcat ccgcctgctg ccaattacgg
    14221 ctgatgcggc tgcgccaaac cgcaatcccc caaccgcttc cgccatgcag caaaaccctg
    14281 tccgcaccga ccgtccccgc gccgcctatt ccgttggata atgcccacgc ctcacggttc
    14341 aaaccgcgca ggttcaactc cccgacgaca gggtgcacac gtgccgttat ccgccatctg
    14401 ctcccgaccg cccattcgcg ccgtttgtag tccgacagca gcaaatcaaa cgtccgacca
    14461 ccgctgtcca cagcctttgc cgcaaactgc acacgccgcc cgtccgacct cggcatatcc
    14521 gccacttcca ccgtcaaagg cacgcctgaa acggcttctg cccaccattg ggaagacaat
    14581 gccgcctccg tcctgaatac gccgtaagcc gcgcccgcca acacgcacag catcagaccg
    14641 gcaaacgcaa aacgccttgc aaacacagca aaaaccgcaa aagccgccag ccaaaacagc
    14701 caatgcggca cgaccggcag cgcaaacgaa gccgccgcac cgacaaccca acacggcaaa
    14761 aaccaacgac ggaacacgtc tccccacccc agatatttca aaaataaacg gataataaag
    14821 caaatccgaa aaaccgtcat aacaagattt gaaaatgata tagtggttta aatttaaatc
    14881 aggacaaggc gacgaagccg cagacagtac aagcagtacg ataaggcgag gcaacgctgt
    14941 actggttttt gttaaaccac tataagaatg caaacgaaaa gcctcagtat tatccgagac
    15001 aacggcaaac tttccatcgt cattcccacg aacccacatc ccgttattac cacgaaagcg
    15061 ggaatctagg ac
//
LOCUS       DAWKQJ010000036        14904 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000036 DAWKQJ010000000
VERSION     DAWKQJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.036"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WJL20_000834"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WJL20_000834"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7683005.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAASPKAEAPVAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAK
                     GRKVDVIQGDGQFLDSHHLEVSLTAGDAYELAAPTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1968..3566)
                     /gene="aceF"
                     /locus_tag="WJL20_000835"
     CDS             complement(1968..3566)
                     /gene="aceF"
                     /locus_tag="WJL20_000835"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ7683006.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGASAAEAAPAVAAEAQPAPAAAPV
                     AAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGV
                     VKAVFLKVGDKVSEGSAIIEVETAGSVAAAPAQAAQAAAPAPTAAAAPAAAPAPSAPA
                     AAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGA
                     AKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTV
                     HEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLML
                     KNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACF
                     TISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVI
                     DGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3716..6379)
                     /gene="aceE"
                     /locus_tag="WJL20_000836"
     CDS             complement(3716..6379)
                     /gene="aceE"
                     /locus_tag="WJL20_000836"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ7683007.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7073..8785
                     /locus_tag="WJL20_000837"
     CDS             7073..8785
                     /locus_tag="WJL20_000837"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ7683008.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLDLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFRGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8848..9504
                     /locus_tag="WJL20_000838"
     CDS             8848..9504
                     /locus_tag="WJL20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ7683009.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTS
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9514..9969)
                     /gene="ruvX"
                     /locus_tag="WJL20_000839"
     CDS             complement(9514..9969)
                     /gene="ruvX"
                     /locus_tag="WJL20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ7683010.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9962..10510)
                     /locus_tag="WJL20_000840"
     CDS             complement(9962..10510)
                     /locus_tag="WJL20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ7683011.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10512..11072)
                     /locus_tag="WJL20_000841"
     CDS             complement(10512..11072)
                     /locus_tag="WJL20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ7683012.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11400..13205
                     /locus_tag="WJL20_000842"
     CDS             11400..13205
                     /locus_tag="WJL20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7683013.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13325..14809
                     /locus_tag="WJL20_000843"
     CDS             13325..14809
                     /locus_tag="WJL20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ7683014.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKLIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQEKTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3542 a         3688 c         4202 g         3472 t
ORIGIN      
        1 ccggaagaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgcagc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgagaccgat aataccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgccagttc gtacgcgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acatccactt tacggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcagcaaat gcagcggagt
     1501 aaccgccggg accgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagcg ggagcggcag ctttgggtgc ttcttgcgca ggggcagcaa
     1621 caggtgcttc ggctttagga gaggcagccg cgccttcggc ttcaacgact acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccaaggt aatcagggtg tcgtcaacgg
     1801 cgatggtatc gcccacattc acttcaaccg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaagctca tgtctcatct ttctgaatga tgttcaaact
     1921 tccttttcag acggcacagg ccgctatgcc gtctgaagcc gatcgggtta cagggtaatg
     1981 cggcggaagt ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg
     2041 tcgatgacac ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct
     2101 ttgccgttcc aaaccggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga
     2161 gcgttcacaa tcggcgtgaa gcctgtgccg ccgatgccgc ccaagctgga aatggtaaag
     2221 catgcgcctt gcatttcttg tggtttgagc ttgccttcgc gggctttttt ggacaattct
     2281 gtcagctctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca
     2341 accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcatcagg
     2401 ttgtcgccgt ccagagaagc gttgaattcg gggaaggctt tcagcgcgga aacggaggct
     2461 ttgatgatga acgccagagg agacagtttc acgccttcgc gttcccattc tttgttcagt
     2521 tgtttgcgga attcttccag ctcggtcata tctgcttctt cgtgtacggt aacgtgggga
     2581 atcaccaccc agttgcgaga caggttttga ccggagattt tcttaatgcg ggacaattct
     2641 ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag caagtccaga
     2701 ccgctgccca aagatgcgcc ggctgcggca ggttttgccg cgccaccctg cattacggat
     2761 ttcacaaagg ctttgatgtc gtcgcccacg atacggcctt tcaagccggt acctttgact
     2821 tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaagggcc ggcgtgtgct
     2881 ttggcgaaag cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg
     2941 gcgggtgcgg cggcagcagt cggagccggt gcggcagctt gagcggcttg agcaggagct
     3001 gctgcgacag agccggcggt ttccacttcg ataatggcag agccttcgga cactttgtcg
     3061 ccgactttta agaatacggc tttaacgacg ccggcagcgg tacaaggtac gtccattgtc
     3121 gctttgtcgg tttccaaagt aatcagcgtg tcgtcttcgg caacggtgtc gcccactttg
     3181 atttcaacgg cgattacatc cacatcggta tgaccgccga tatcgggaac ggctacttga
     3241 acggttgcac cgcctgcggc aacgggtgcg gcggcaggtg caggttgtgc ttcagcggca
     3301 acagccggag cggcttcggc ggcggaggca ccggtttcaa cggtcaaaat tacgccgcct
     3361 tcggagattt tgtcgccgac tttgactttt acttctttca cgacaccggc agcatcggca
     3421 ggcacatcca tcgtggcttt gtcggtttcc agtgtaatca gggtgtcgtc aacggcgatg
     3481 gtgtcgcccg ctttaacttc tacggcgatg atgtcgacgt tttcgtgacc gccgatatcg
     3541 gggactttga tttctacgat actcatttga gttcctttaa tcttgttcag ttggatgccg
     3601 tctgaaagct gcttcaggcg gcatcggtac ggtttgtctg ctcaaaccat tcgggcattc
     3661 ggttttcaga cggcctgatg tcggaatggg gcaaacaggc cgtctgaaac atcaatcagc
     3721 gtttccagct aggagctgaa tcggctttga tgccgtattt ctcaatggct tgttgaacgg
     3781 tttctttgct gactttgcct tgttccgcca atgcggccaa tgcggccacg gcaacgttgt
     3841 agcgatccac ttcaaagaag cggcgcaggt tggcgcggct gtcggagcgg ccgaagccgt
     3901 cagtacccaa gacgtggtag tcgttcggga tgtaggcgcg gatacggtca gcatagctgc
     3961 ggatatagtc ggtagcggca ataaccggac cgtcatgacc ttgcaatttg gaagtaacga
     4021 aaggtacttt ttcggcttcc agcggatgca ggcggttgaa gcgttctacc tcgacagcgt
     4081 cgcggtgcag caggttgaag gatgggcaag accaaatgtc tgcttccacg ccgaagtcgg
     4141 ctttcagcag ttcggcaccg gcaatcactt cttgcaaaat cgtaccggaa cccatcagtt
     4201 ggactttctt gtcgcctttg ccgccggctt tcagcaggta catacctttc aagatgtctt
     4261 gttccgcacc ttcgggcata tccggatggg tgtagttctc gttcatcagg gtgatgtagt
     4321 agaacacgtc ttcattattg gcatacatac ggcgcagacc gtcttgtacg atgacggcga
     4381 cttcgtattg gaaagtcggg tcataagata cgcagttcgg aatcaggtcg gcctggatgt
     4441 ggctgtggcc gtcttcgtgt tgcaggcctt cgccgttcag cgtcgtacgg ccggcagtac
     4501 cgcccagcag gaagccgcgc gcgtgcatat cgcccgccgc ccaagccaag tcgccgatac
     4561 gttggaaacc gaacatagaa tagtaaatgt agaacggaat catggcgaag ttgctgttgg
     4621 cgtagctggt tgcagccgca atccagtcgg ccatcgcgcc cggttcgtta ataccttctt
     4681 gcaagatttg accgtcaacg gattctttat agaacatcag ttggtctttg tcttgagggg
     4741 tatattgctg acctttcgga ttccaaatac cgtattggcg gaacatacct tccatgccga
     4801 aagtacggct ttcgtcggga acgataggta cgacgcgttt gccgattttt ttgtctttca
     4861 gcaaagtgga caggatgcgg acgaatgcca tcgtggtcga gaactcgcgt tcaccgctgg
     4921 atttgagttg tgcgtcgaat gctgacagct cgggcacttc caatacttcc tgcgtcggtt
     4981 tgcgttgcgg caggtagccg cccaaagcat cgcggcgtgc gtgcaggtat ttgtattctt
     5041 ccgtatcggg ggcaaaagtc aggtaaggca gatcgccgct ttcgatttgt tcgtcggtaa
     5101 ccggaatgtc aaagcggtcg cggaattgtt tcagggacgc tttgtccatt tttttggctt
     5161 ggtgggcaac gttctgacct tcgccggatg cgcccatacc gtaaccttta atggttttcg
     5221 ccaagatgac ggtaggtttg ccgtcggcat ggttcgctgc gcggtcgtag gcgttgtaca
     5281 ctttttgcgg gtcgtggccg ccgcggttca atgcccagag ttgctcatcg gtcatatcgg
     5341 caaccaatgc tttcagttcg ggcgtattga agaagtgttc gcgcacatac gcgccgtctt
     5401 tggatttgta agtttggtag tcgccgtcca aacattcttc catacgttgg cgcaggatac
     5461 cgtctttgtc tttcgccaag aggcggtccc agcggcggcc ccaaatgact ttgacgacat
     5521 tccagccggc gccggcaaag ttgccttcca attcttggat gattttgccg ttgccgcgta
     5581 ccggaccgtc caagcgttgc agattgcagt tgatgacgaa aatcaggttg tccaagcctt
     5641 cgcgtgcagc cagtgcgatt gcaccttgag attcgggttc gtccatttcg ccgtcgccgc
     5701 agaaacacca tactttacgg cctttggttt ttgccaaacc acgcgattcc aagtatttca
     5761 ggaaacgcgc ctgataaatc gccatgatgg gccccaagcc catggatacg gtcgggaact
     5821 gccaaaagtc gggcaagagg tggggatgcg gataggaagg cagaccgtgt ccgtccactt
     5881 cttggcggaa gttgttcagc tggtcttcgg tcagacggcc ctcgacgaat gcgcgtgcat
     5941 agatgcccgg ggcgacgtga ccttggaaga agaccaaatc gccttcttcg ccttcgcctt
     6001 tggctttcca aaagtggttg aaaccgactt cgtacatggt ggcggcagat tggaaagatg
     6061 cgatgtgccc acccagttcc aaatctttct tgccggcgcg caatacgatg gcggcggcgt
     6121 tccagcgcac gaatgcgcgg atgcggtgtt cgatgttttg gtcgcccgga atgccttttt
     6181 cgttttcaac cgaaacggta ttcaaatacg gggtggtcgt gccgtgtggc atacgtacgc
     6241 ccttgtcgcg gcagtatttg accaggtttt ccaagaggta ttgcgcgcgt tcgccgcctt
     6301 catattcgag gacggagctt aaagcgtcca gccactcttg ggtttcgata gggtcaacat
     6361 cgtgtaattg ggtggacata gtatctatcc tttatgttga gtgtttaatc ggcagggagg
     6421 gtgtagccaa cccctttatt ttcgtttgtg aaaaataata agcaaaaacg aaaatttcta
     6481 aatcaaaccg aaaaaaataa tttaagttta atgttttcaa aaaattataa aagttttgga
     6541 aacaagggaa aacttgaaaa aaatgttcga tctgctccac acgaaatcct atcagcaatc
     6601 gggtctgctg gcaaataacc atgcctgtaa aagcaggttt tatactgaag ttggcgtttg
     6661 gtaaggtcaa gtctttttga tttcgaaata ttctgtttga ttttggcgat attttttcgg
     6721 aattgtggta tatcgaatca ttatgtctct gtcggcaatt ttcctattgt agaaaatcta
     6781 tacacaagct ttgtggataa ccgtgttgat aagccacggc ttgtgttaaa aaagtcttac
     6841 gcaacaaatg tacgcttatt ttgattaaaa aataggcaca tttaaatcta taaaaatcat
     6901 gtgcttatgt tcaatatgat gtttctggtc atgtttttgc cgtctgttat gggatttgtc
     6961 ccgcgcttgg ggcagggggt gaattttgga ggttggtgca aaacctgctt ttttcgcgtt
     7021 acaatctgca cccgatttac ttttgaatct gcctgtttgg aaaaatactt ttatgaaagc
     7081 cagccaattc tttatctcta ctttaaaaga agcccctgcc gaagccgcgc ttgccagcca
     7141 caagctgatg attcgcgccg gtctgattaa agccaacgcg tccggccttt atacttggat
     7201 gccgatgggg ctgcgcgtgt tacgcaaagt cgaaaacgtc gtgcgcgagg aaatggcgcg
     7261 cgcgggcagc gtggagttgc tgatgccggt ggtgcagcct gccgagctgt ggcaggaatc
     7321 cggccgctgg gagttttacg gtaaagaatt gctgcgcctg aaagaccgcc acgaccgcga
     7381 tttctgcatg ggcccgacct gcgaggaagt catcgccgac atcgtgcgta aagagatcaa
     7441 cagctacaaa caactgccga aaaactttta ccacatccaa accaaattcc gtgacgaagt
     7501 gcgcccgcgc ttcggtgtga tgcgtgcgcg cgagtttgtc atgaaagatg cttattcctt
     7561 ccatgccgac tacgcctcgc ttcagacgac ctatgatgcc atgtatgacg cttactgccg
     7621 tatcttcacc cgtctggact tggcattccg ccccgtcgcc gcagacaccg gcagcatcgg
     7681 cggtacgggt tcgcacgagt ttcaagtgtt ggcggaaagc ggcgaagatg ttattgcgta
     7741 cagcgacact tccgattacg ccgccaatat cgagttggca ccgaccttgc cgcttaaagg
     7801 cgaacgcgcc gctgctcagg ctgtgttgac caaagtgcat acaccaaacg tcaaaaccat
     7861 tgagtctttg gttgaattcc tgaatattcc ggttgaacaa accctcaaat ccatcgtggt
     7921 cgaaggcgaa aacgaaggcg aaatcgtctt attactgttg cgcggcgacc atgagttcaa
     7981 cgacatcaag gcagaaaaac tggcgggcgt gaaatcgcca ttgacgatgg caagttctgc
     8041 cgcgattgtc gaacagttcg gcgcaaacgg cggctctctt ggtcctgtcg gcttcagagg
     8101 caaagtctat gccgatttcg ctaccgaaaa aggcgcggac tgggtcatcg gtgcgaacga
     8161 agacggctac cactataccg gcttcaactt cggtcgcgat gccgccgagc ctgaattcgt
     8221 cgatttgcgt aatgtggtcg aaggcgacga aagccccgac gggcaaggcc gtctgaaact
     8281 ggcgcgcggc atcgaagtcg gacacgtctt ccagttgcgc gacaaataca cccaagccat
     8341 gaacgtgagc ttcctcgaca acaacggcaa atcgcaaatt atggaaatgg gctgctacgg
     8401 catcggcatc acccgcgtcg ttgccgccgc catcgagcag aacaacgacg aaaaaggcat
     8461 catctggacc aaagcaatgg cgccgtttga agtcgttatc gtgccgatga actacaaaaa
     8521 atcagacacc gtacgcgaag ccgccgacaa aatctatgcc gaattgctgg cggcaggcgc
     8581 ggatgtgctg ctggatgacc gcgacgaacg cgcaggcgta ttgctgaacg attccgagct
     8641 gttgggtatt ccgcaccgca tcgtcatcgg cgaccgcgct ttgaaagaag gcaatgtcga
     8701 atacgccgaa cgccgcaaca acgaagcgca ggcagttgca atcggagaaa ttgttgcgcg
     8761 tgtaacagct tcattaaatg ggtaagcttg gtcaaatgcc gtctgaaaat aatttcagac
     8821 ggcattttta tttggggaga aagtgaagtg gcttcagtat ttttagaagg agaggcagtc
     8881 gaggtcggca atattttctg tatcggcagg aactatgccg cacatattga agagttaaaa
     8941 aacgaaatac cttccgaacc tgtggtgttt atgaagccgt caggcagcat tttgaatagc
     9001 ggcggtacaa tcttgctgcc cgagtttagt agggatgtgc agtttgagtg cgaactcgtg
     9061 ctgctggtcg gtaaggattc agacggcacg ggcgagggta aagatatttt gggatgtgtc
     9121 gcaggatatg gtgtagggct ggaccttacg gcacgggata tccaatgccg tctgaaggaa
     9181 aaagggctgc cctggctgaa ggcaaaaggg ttcaggcatt ccgcgtgcgt gtcggatttt
     9241 gcggcggcag gcagaatcgg caatcccgag aatgtgttgt tttcattaaa acaaaacggc
     9301 gtattgaaac agcgcgggga taccagtttg atgatttacc cgattcggga aattttacac
     9361 aaactggcgg cagattacgg tttggggaaa ggggatttgg tgtttaccgg aacgccctcc
     9421 ggcgtgggcg caatcggtgc gggggacaat ttggcattgg agctggacgg cttggtgcgc
     9481 gcctctttca cggtagggtg ttaaccgcgc cgcttaaccc tcacgcccgt tgaaacattc
     9541 cgccggaccg ccctcgaaaa aaccgtgcaa gatggcttgc gccgccactt ggtcgagcac
     9601 cgatttgcgt tttttgccga agacctgtgc ttccgaaagc aggctttcgg catagacgga
     9661 cgacagccgt tcgtcaaccc aatagacggg gagattgaac ctgccgttca gcctgcgtcc
     9721 gaacttgcgc gacaggtgcg tcatttcatg tttcgtgccg tcggtatgca cgggcagtcc
     9781 gacgacaaaa taacgcggct gccattcttg aaccagcttg gcgattgccg cgaacttttc
     9841 atcgttgctg ccgccggtaa cggtgctcaa aggatgggat agccctaatt ccgcgtctcc
     9901 ttgtgccacg ccgatacgcg cttcgccgaa gtcgaatgcc agtgccgttc cttttggaat
     9961 tttatgcatg gccggctccc gaaaacaggg cgagcgggtc gatgccgagt ttggcgaatg
    10021 cggcggcgta acggtgttcg taggggatgt cgaacaggat gtgttcgtcg gcgggaacag
    10081 tcagccacgc attgtcggca agttcgcgtt cgagctggcc ttttccccag cttgaatagc
    10141 ctatgctgat caaggctttg tcaaccgcac cttcgcgtga aatattttca atcacgtctc
    10201 gggaagaagt tagcgcgata ttgtctgaaa cgccgatact gctttgccag ttgccgatcg
    10261 gggtatgcac gacataacct cgctcgactt gcaccggccc gcccatcatc acgctgtcgt
    10321 gctgcatccg catggggatg tttttgccgg tggcggaaaa aatcatgtcc atcgtaatcg
    10381 gagagggttt gttgatggcg atgccgagtg cgccgtcttc atcgtgtttg cagatataga
    10441 cgaccgattg tgaaaaaaac gcgtcttcca tatcgggcat ggcgacgaga aagtggttgg
    10501 agaggttcat atcagcgttt gtcgggattt tctatcgggt tggaaatcat gcaggcgccg
    10561 gcaagcaggt tgccggaagt atcggcttgc accccatcgg tttgcggtat gccgccgccg
    10621 aaacaggaaa ccgcgcttaa ggaatttttc ggcgagcctt ggatgatttt atcgctgtac
    10681 accaaatagg cgaaggtttt gcgtttgggg tcgtaataac ggacaatctg ccggctcttg
    10741 aacgcgaagc tcgcaccgtg tttgaaaact tctttcggtt tgcgcacggc ggtttcgtca
    10801 aaagaaatcg aagatgccgt ctgaacgcac gaaaccgatg cgtcggacgc gtcctcttcc
    10861 aaattgacca tttccttcaa gccgcctttt tttgcatacg aaatataaca ggcaacccct
    10921 tgaacgtcgg gatcgtcgaa tccttccact tcgatacggt cgtttttgcc cagtatgttg
    10981 aaaacggtac ttgcccgtcc gattttatcg gtttcgccgc tgccgcaggc agtcagcagg
    11041 acggcggcag acagcagtag cagtctgttc atcaaaattt ccttatcggt tgaatccccg
    11101 ttttccgtga aggcggcagg gcttgctttg tttggagggg cgcacgttcc caaaatcggg
    11161 caacatacgg tatgcggctt aatgtgctac cctgtgtgtc ggaacacaat atccgttgcg
    11221 gcattgaaaa tatgatgcac aatataaggc tgtttgagcc gattgcaagt atccgcgcaa
    11281 agaaggcgca agattgggca aacggcattt tcagacggca taactgacag tataatccaa
    11341 acgtccggct gctttcgtgc agccttcagt attatccaac tttttccgaa ggtcgaacca
    11401 tgcgctacaa accccttctg cttgccctga tgctcgtttt ttccacgccc gccgttgccg
    11461 cccacgacgc ggcacacaac cgttccgccg aagtgaaaaa acagacgaag aacaaaaaag
    11521 aacagcccga agcggcggaa ggcaaaaaag aaaaaggcaa aaatggcgca gtgaaagata
    11581 aaaaaacagg cggcaaagag gcggcaaaag agggcaaaga gtccaaaaaa accgccaaaa
    11641 accgcaaaga agcagagaag gaggcgacat ccaggcagtc tgtgcgcaaa ggacacgaag
    11701 gggataagaa atcgaaggcg gaacacaaaa aggcacatgg caagcccgtg tccggatcca
    11761 aagaaaaaaa cgcaaaaaca cagcctgaaa acaagcaagg caaaaaagag gcaaaaggac
    11821 agggcaatcc gcgcaagggc ggcaaggcgg aaaaagacac tgtttctgca aataaaaaag
    11881 tccgttccga caagaacggc aaagcagtga aacaggacaa aaaatacagg gaagagaaaa
    11941 atgccaaaac cgattccgac gaattgaaag ccgccgttgc cgctgccacc aatgatgtcg
    12001 aaaacaaaaa agccctgctc aaacaaagcg aaggaatgct gcttcatgtc agcaattccc
    12061 tcaaacagct tcaggaagag cgtatccgcc aagaacgtat ccgccaagag cgtatccgtc
    12121 aggcgcgcgg caaccttgct tccgtcaacc gcaaacagcg cgaggcttgg gacaagttcc
    12181 aaaaactcaa taccgagctg aaccgtttga aaacggaagt cgccgctacg aaagcgcaga
    12241 tttcccgttt cgtatcgggg aactataaaa acagccagcc gaatgcggtt gccctgttcc
    12301 tgaaaaacgc cgaaccgggt cagaaaaacc gctttttgcg ttatacgcgt tatgtaaacg
    12361 cctccaatcg ggaagttgtc aaggatttgg aaaaacagca gaaggctttg gcggtacaag
    12421 agcagaaaat caacaatgag cttgcccgtt tgaagaaaat tcaggcaaac gtgcaatccc
    12481 tgctgaaaaa acagggtgta accgatgcgg cggaacagac ggaaagccgc agacagaatg
    12541 ccaaaatcgc caaagatgcc cgaaaactgc tggaacagaa agggaacgag cagcagctga
    12601 acaagctctt gagcaatttg gagaagaaaa aggccgaaca ccgcattcag gatgcggaag
    12661 caaaaagaaa attggctgaa gccagactgg cggcagccga aaaagccaga aaagaagcgg
    12721 cgcagcagaa ggctgaagca cgacgtgcgg aaatgtccaa cctgaccgcc gaagacagga
    12781 acatccaagc gccttcggtt atgggtatcg gcagtgccga cggtttcagc cgcatgcagg
    12841 gacgtttgaa aaaaccggtt gacggtgtgc cgaccggact tttcgggcag aaccggagcg
    12901 gcggcgatgt ttggaaaggc gtgttctatt ccactgcacc ggcaacggtt gaaagcattg
    12961 cgccgggaac ggtaagctat gcggacgagt tggacggcta cggcaaagtg gtcgtggtcg
    13021 atcacggcga gaactacatc agcatctatg ccggtttgag cgaaatttcc gtcggcaagg
    13081 gttatatggt cgcggcagga agcaaaatcg gctcgagcgg gtcgctgccg gacggggaag
    13141 aggggcttta cctgcaaata cgttatcaag gtcaggtatt gaacccttcg agctggatac
    13201 gttgatcgga ttgccgaaac ggtaggcttt atgcaaatcg tgccgatatg gtgcgctgtt
    13261 ccgtgaagtt tgccgtacct gtattttccg gtttttggaa atcaagagag aaagttaaag
    13321 aagaatgtcg aaacctgttt ttaagaaaat cgcactttat actttgggtg caatcagcgg
    13381 cgtggccgtc agtctggcgg tgcagggttt tgccgccgag aaggacaggc gggataacga
    13441 agtcctgccg gtgcaatcca tccgcacaat ggcggaggtt tacggtcaaa tcaaggcaaa
    13501 ctactatcag gacaaacccg atgccgattt gtttgaaggt gcgatgaagg gtatggtggc
    13561 cggtttggat ccgcattccg aatatatgga taaaaaaggt tatgccgaga taaaggagtc
    13621 caccagcggc gaatttggcg gcttggggat ggaaatcggg caggaagacg gatttgtcaa
    13681 agtggtttcg ccgattgagg acacgcctgc ggaacgggcg ggggtgaaaa gcggcgattt
    13741 cattgtgaaa atcgataatg tttcgacacg cggcatgacg gtcagcgaag cggtgaagaa
    13801 aatgcggggc aagccgggta cgaagattac tttgacgctg tcgcgcaaaa atgccgacaa
    13861 gctgatagtc gtcaacctga cccgtgccat tattaaagtg aaaagcgtcc gccatcacct
    13921 gatcgaaccc gattacggct atatccgcgt gtcgcagttc caagagaaga cggtcgaaag
    13981 cgtcaatacc gccgcaaaag agctggtaaa ggaaaataaa ggaaaaccgc tcaaggggct
    14041 ggtgttggat ttgcgcgacg accccggcgg gcttttgact ggcgcggtcg gcgtgtcggc
    14101 ggcatttctg ccgtctgaag cagtcgtcgt cagcaccaag ggacgcgacg gcaaagaccg
    14161 catggtactg aaagccattc ctgaagatta tgtgtacggg atgggcggcg attcgttggc
    14221 gggcattcct gccgagttga aaaccatacc gatgacggta ttggtcaatt ccggttcggc
    14281 ttccgcgtcg gagattgtcg caggtgcatt gcaggatcat aaacgcgcgg tcatcgtcgg
    14341 tacgcagagc ttcggcaaag gttcggttca gactttgatt cctttgtcca acggcagcgc
    14401 ggtcaagctg acaacggcac tgtattatac gccgaacgac cgttctattc aggcgcaggg
    14461 gattgttccc gatgtcgaag taaaagataa ggaacgcatt tttgaaagcc gcgaggcgga
    14521 tttggtcgga cacatcggca atcccttggg cggcgaggat gtgaacggtg aaacccttgc
    14581 cgtgccgctt gaaaaagatg cggataagcc cgctgtaaaa gaaaaaggta aaaagaaaaa
    14641 ggacgaggat ttgtcttcaa ggcggattcc caaccctgcc aaagacgacc agttgcggaa
    14701 agctttggat ttagtcaagt cgcccgagca gtggcagaag tctttggggc tggcggcgaa
    14761 aaagccggtt tcaaataaag ataagaaaga taaaaaagat aagaagtagg ttttgacgat
    14821 gccgtctgaa aggatttcag acggcatatt atgtttgaaa ggacaggtta tgagcgattt
    14881 tttaccggaa tttgaaccgt gcgg
//
LOCUS       DAWKQJ010000037        14898 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000037 DAWKQJ010000000
VERSION     DAWKQJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.037"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(101..3586)
                     /gene="smc"
                     /locus_tag="WJL20_000844"
     CDS             complement(101..3586)
                     /gene="smc"
                     /locus_tag="WJL20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ7683015.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQVTGHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPYGFVPPTPLPQGQAAWL
                     SDGLSSGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLSPELSSAEAAFKQAE
                     ATVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLVRTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIAILQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVQTLDEEYIAVQEKLAQIQAQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNTSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3588..3839
                     /locus_tag="WJL20_000845"
     CDS             3588..3839
                     /locus_tag="WJL20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683016.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3836..4558
                     /gene="lpxH"
                     /locus_tag="WJL20_000846"
     CDS             3836..4558
                     /gene="lpxH"
                     /locus_tag="WJL20_000846"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ7683017.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4827..6413
                     /locus_tag="WJL20_000847"
     CDS             4827..6413
                     /locus_tag="WJL20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7683018.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6581..6784)
                     /locus_tag="WJL20_000848"
     CDS             complement(6581..6784)
                     /locus_tag="WJL20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ7683019.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6900..7397
                     /locus_tag="WJL20_000849"
     CDS             6900..7397
                     /locus_tag="WJL20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ7683020.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7479..8672
                     /locus_tag="WJL20_000850"
     CDS             7479..8672
                     /locus_tag="WJL20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7683021.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9076..10011)
                     /gene="hemC"
                     /locus_tag="WJL20_000851"
     CDS             complement(9076..10011)
                     /gene="hemC"
                     /locus_tag="WJL20_000851"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ7683022.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10130..10645
                     /gene="ybeY"
                     /locus_tag="WJL20_000852"
     CDS             10130..10645
                     /gene="ybeY"
                     /locus_tag="WJL20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ7683023.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10647..11471
                     /locus_tag="WJL20_000853"
     CDS             10647..11471
                     /locus_tag="WJL20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ7683024.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(11614..12993)
                     /gene="nhaC"
                     /locus_tag="WJL20_000854"
     CDS             complement(11614..12993)
                     /gene="nhaC"
                     /locus_tag="WJL20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ7683025.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13284..14507)
                     /locus_tag="WJL20_000855"
     CDS             complement(13284..14507)
                     /locus_tag="WJL20_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ7683026.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3512 a         4196 c         3844 g         3346 t
ORIGIN      
        1 caacgctgta ccgatttaaa tttaattcac tataccgtct gaaaacctgt atcgcaacac
       61 gtcattaaga acggcaagat tcaaccgttc tgcaacccgt tcaaacggct tccgccattt
      121 ccaacgcttg tttgatgtcc acggcgacga cgcgcgatac gcctttttcc tgcatcgtta
      181 cgccgacgag ctgctctgcc atttccatcg tcaggcggtt gtgggagatg tagaggaact
      241 gggtttgcgc cgacatttct ttgaccaggt tgcagaagcg cgaggtgttg gcgtcgtcca
      301 gcggggcatc gacttcgtcc aaaaggcaga acggagcggg gttgaggctg aacagagcga
      361 acacgaggct catggcggtg agggcttttt cgccgccgga gaggaggtgg atggtgctgt
      421 ttttcttgcc gggcggacgc gccataatgg acacaccggc ggtcagtagg tcgtcgccta
      481 tcattttgag ggtggcttcg ccgccgccga acagggtcgg gaagaaggtt tggactttgc
      541 tgttgacggc atcgaaggtt tctttgaaac gcgctttggt tttgtcgtcg atttgggcga
      601 tggcttcttc caaaagagta atggctgcct gcacgtcttc gctctggctg cggtagtagc
      661 cgtcgcgttc gcgcgcttct tcgagttctt gcagggcggc gaggttgacc gcgccgaggg
      721 cttcgatttg ttgcgaaagg ctgccgatgc tggtgttcaa tactttcggc gattctttcg
      781 ccaacgcttc gagcgcgtcc aaatcggcgg cgcgttcggt caggttttga tggtagcgtt
      841 tggcgttgat cagggcttcc tgctgctgca acagggcggt ttgggtagcg gcctgaagct
      901 gcggcagctt ggtttgcagg gtttgcactt tagcgtattg ctccctgccc tgcgcctgaa
      961 tctgcgcgag tttttcctgt acggcgatgt actcttcatc caaagtttgt acggcttcgg
     1021 tcaactcgtc gagttttatg tgctgctcgt cgttttggaa ctcggtttca taggcgaggg
     1081 caagctcttg ctggcgttcc tgccaatcga gggtttgctg ttcgagtcgg gcgatttgct
     1141 gttggtagtt ttgtttttgc tggttgagct tgtggacggc gacttcggca agcccgtatt
     1201 ggcggttggc ttccagcagg gcaagctgcg cctgtttcag acgaccttgc tgctcttggc
     1261 ggctgtgtgc ggtggtttgc tgctggtgtt cgagttcggc ggcggcttcc tgcaaaatgg
     1321 cgatgtcgtc tgaaagcccg tcggacgtgt gttgcaacac ggtctgctct tccgccaact
     1381 gcgccagttc gcgctcgatg tgttcgcggc ggatttgccc ttggttggtg cgcaccagaa
     1441 gttcggcggc gcgttgctgc gcttggctgt attggcgcgt gtgctgctgt tgctgctgca
     1501 tcaggttttt atgttgcact tcggacgagc gcacggttgc ctccgcctgt ttgaacgcgg
     1561 cttcggcgga ggagagttcg ggggagaggt tttccagttc ggacgcgatg ccgtcgaggc
     1621 gcgctttttg ggcaatcagg ctttcctgcg cgggtttggc atagagcagg acgctgacct
     1681 tatcgacctg atgaccttcg ggcgtgagcc agatttggtg tgcgcccaaa tcgctttgat
     1741 gcgcgagggc atagctcaaa tcgggcgcgc acaatacgcc gtcgagccag tggtgcaatg
     1801 ccgtctgaaa cggcggctgc gcttggattt ggttcagcaa tgcctgtacg ggcagggatt
     1861 ttttgatgcc gctggagagg ccgtctgaaa gccatgccgc ctgcccctgc ggcaaaggcg
     1921 tggggggaac gaagccgtac ggtacggcgc gggcgtgcag gcgttcggca aggatgacgg
     1981 acaaggcgtg ttgccactcg gcgggcgcgg tgatgtgttg ccacagttgc ggcgcggcgg
     2041 cgtggccggt tacctgccag aaatcggcgg cttcctgctg ttgcgacagg atttgcgaca
     2101 acgcctgctg ctgcgcctgc aaggtgatat gttgctgctt caggctttgg aagcggtttg
     2161 atgccgtctg aaacgcctcg cgggcggcgt gtagagcttc ttcggcggcg atgatttgtt
     2221 cttcgtaatg ctcttgctgg ctttgcaaca aggcggcggc ttcctgcgcg gcggcggttt
     2281 cggcttcgtc gggcaggttc agggcttggt tttcctgttt cagacgacct ttgcgctctt
     2341 cgtgcttggc aacggtttgt tcggcatggg caagctgctg ctgcttcaac gccagttcgc
     2401 ggcggatgcg gtttgcctcg tcctgctggg tttggaaggc ggcattgagc gtggcttggg
     2461 cttcttccaa ttcgggcaga cgctcttcgt gttcggcaac ctgcatcgcc cattccgcca
     2521 gctcggtttg tttttcttcg acctgcaact cgttttcttc aagctgcacg cggatttgct
     2581 gctgctcttg atgaatgcgt tgtaactgcg cctgcgccgc ctgcttgtcg cgttcgatgc
     2641 gttgatgcag gttttgctga tggcggattt gttcttccaa acgggcaatc tgctcgcgca
     2701 acacgccgcg tttgttgccc agttcgtgta ccgcctgctg ctgcgactgt tcggcggtct
     2761 gcaaggcgtg tacttcgtcg tttaacgcct gaacctgcgc ggcggtttcg tcctgctgcg
     2821 cctgcaaaga ctgatgctgc gcggtcgctt tgtcggcggc ggcaagcgat tgctgccatt
     2881 gggcgtaatc gagcaaatcc tgttgccggt tcaactgcgc ggtcagggat ttgtagcgtt
     2941 cggcggtttc cgcctgtttc tccagctttt ccacctgacg cgccaattcg ttctgcaaat
     3001 cgcccaaacg ctgcaaatgc tcgcgcgtgt ctttcagacg gccttccgtc tccttgcggc
     3061 gttccttata tttggacaca cccgccgcct cctcgatata agcgcgcaac tcctccggcc
     3121 gcgcttcgat gatgcgcgaa atcatcccct gctcgataac ggcataaccg cgcgcgccca
     3181 cgcccgtacc cagaaacaaa tcagtaatat cgcggcggcg cacggtctga ttgttgatga
     3241 aataagtcga ttcgccctgc cgcgtcagct gccgcttgat gctcacctcg gcatactgcc
     3301 cccacgcgcc ctgcaaactg tggtcgctgt tgtcaaacac cagctccacc gaagccctcg
     3361 gcgcaggacg gcgcgtcgcc gcaccgttaa aaatcacgtc ctgcatactc tcgccgcgaa
     3421 gctgcttcgc cgaagcctca cccaacaccc agcgcaccgc gtcaatcaca ttcgacttgc
     3481 cgcaaccgtt cggcccgata accgcgacaa gctgccccgg cacatgaatt gtggtcgggt
     3541 cggtaaaaga tttgaagccg gagagtttga tatgggtcag gcgcataatg gtcggaaaaa
     3601 ataaaaaaga agcgtatttt aacggaaatc ccgccgcacc accccatatc ttgccggcaa
     3661 aaccttaccg catcccgcac cctcgatgcc gtctgaagcc ttcagacggc atttttaacg
     3721 cgcccgaacc ccgattccgc caacgcccga tgcttggcgc ggcatttgcc gtattgcccc
     3781 gccgccgggc gcgttataat ccgccacgac atccgcccga tttggaaacc tgcctatgaa
     3841 acccgcctat ttcatctccg acctgcattt gagcgaaaag caccccgaac tgaccgcgct
     3901 gttgctgcgt tttttacgtt cttccgccgc cgggcaggcg cgggcggttt acattttggg
     3961 cgatttgttt gatttttggg tgggcgatga cgaagtttcc gagttgaata cttcggttgc
     4021 gcgtgaaatc aggaaattgt ccgacaaagg cgttgccgtg ttcttcgtca ggggcaaccg
     4081 cgacttcctg attggtcagg atttttgccg gcaggcaggc atgacgctgc tgccggatta
     4141 ctcggttttg gacttgttcg gctgcaaaac cctcatctgc cacggcgaca ctctgtgtac
     4201 ggatgacagg gcataccaac gtttccgcaa aatcgtgcat cggaagcggc tgcaaaaact
     4261 gttcctgatg ctgcccctga agtggcgcac gcgccttgcc gccaaaatca ggcgcgtcag
     4321 caaaatggaa aaacaggtca agcccgccga tattatggat gtcaatgccg cctttaccgc
     4381 gcggcaggtt cgcgccttta acgcggaaag gctgatacac ggacacaccc accgcgagca
     4441 tatccatcac gaaaacggct ttacccgcat cgttttgggc gactggcata acgactatgc
     4501 ttcaatcctc cgcgtggacg gggacggcgc ggtattcgtg ccgctggaaa aatgctgaaa
     4561 atgccgtctg aagcctttcg ggcggctttt tttacacgcc cctttcccac actccctccg
     4621 ccgcatcatc accatcctta acccgcatta atgggcgtta tcgataaaat gcttgaccga
     4681 ttacataaga ttacgtaaag tgtgcaaacg cataacaatt ggtcttacca atttatcttt
     4741 taaccaaaat atcgggtgta cgggatttga acgtctttcc aaacctcgac gcgccttatg
     4801 atgcaaatgc ctaaaaagga ttcgttatgg cacttttcct cagcatattc cccatcgtcc
     4861 tgctgatttg gctgatggtg aaaaaaaaca gtatgccctc ctacgtcgcg ctgccgatta
     4921 ccgcagtgct gatttacgcc atcaaacttt tctacttcgg cgatgcgggc atgctgctca
     4981 acgccaccgc cgcttccggc ctcgtcaaaa cgctcacgcc gattaccgtg attttcggcg
     5041 cgattatgtt caaccgtatg atggaaacca cgggctgcat tgatgtcatc cgcaaatggc
     5101 tggcgaccat cagccccaac cccgtagcgc aactgatgat tatcggctgg gcttttgcct
     5161 ttatgattga aggcgcatcc ggcttcggca cgcctgccgc gattgccgcg ccgattctga
     5221 tgagcttggg cttcaacccg ttgaaagtgg cgattttcac tttggtgatg aactccgtcc
     5281 ccgtctcttt cggcgcggtc ggtacgccga cctggttcgg tttcgcaccg ctgaacctga
     5341 gtgccgaaga catcctcgcc atcggcaggc agaccggcgt aatgcacttc ttcgcaggtt
     5401 tcgtcatccc cgtcatcggc ttgggcttca tcgtaccttg gtctgaaatc cgcaaaaact
     5461 tgggcttcgt cgccattgcc gtcttctcct gcaccattcc ttatgtcgca ttggcgatgg
     5521 tcaacgaaga attcccgtcg ctcgtcgccg gcgcaatcgg cctgatggtg tccgtattcg
     5581 ccgccaacca aggctggggc ttgagcaaag accacgccaa agacccgaat gccgaaaaag
     5641 tgccgttcgc ccaagtcgcc aaagcactcg cccctttggg tatgctgatc ggcatgctgg
     5701 tggttacgcg catcaaacag ctcggcatca aaggcatttt gaccagcaaa gaagaatggt
     5761 tcagcttcca actgccgttt gatttgtcca aaatcaccgt cagcgactcc ctgacgatta
     5821 ccttcggcaa tattttcgga caagatgtca gcgcgtctta ccaaacgctg tacgtcccgg
     5881 cttggattcc gtttgtgctg accgtttgga tttgcatcct gctgtataaa accaaattca
     5941 aagatgcctg gacgatttat tccgtaactt tcaatcaaac caaaaaaccg ctgcttgccc
     6001 tgatgggcgc gctgattatg gttcagctga tgctggtcgg cggcgacaat tcgatggtga
     6061 aaatcatcgg taaggaattt gccgcaatgg cgggcgaaca ctgggtttat ttctcgccgt
     6121 atctgggcgc gatcggtgcg ttcttctccg gctccaacac cgtgtccaac ctgactttcg
     6181 gcccgattca gcagcaaatc gccttggata ccggcctgtc cgttaccctg attctggcgt
     6241 tgcagtccgt cggcggcgcg atgggcaata tggtgtgcct caacaacatc atcgccgtat
     6301 gtaccgtatt ggatgtgaaa aattccgaag gtgcgattat caagaaaacc gttatcccga
     6361 tggcgattta cggcgtgatt gccgtcgtcg cggcaatgat tttcttcctc taggcgaaac
     6421 gccaccatcc atagcggcag cacgcgggga acggaaaccg ccgttccccg tctgggcggc
     6481 gcgtcgggca gaatcccggt gccggaacag cccgccccgc caaacaaatg ccgtctgaaa
     6541 ccggaaaagg cttcagacgg catttttccg ccgtttgcat tcaggcgtaa tgatgatgcg
     6601 gcatcttgcc gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt
     6661 ctccttcgca caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc
     6721 ccgagcaata cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt
     6781 ccatttgatt ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa
     6841 ggatattttc ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca
     6901 tgactgcgct tacccttccg gaagacatcc gccaacaaga gccatccgcc ctgctctatc
     6961 ccctcgtttc cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc
     7021 tgtccgacga ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag
     7081 gcggcttcgt gcaactcatc gcctccggtt acggcgaata tattttccgc aacccgcttg
     7141 ccgacagcct gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac aaagccaaag
     7201 ccctctacga acaacacggc aaaaccatcg aaacgctcgc cgacggaggc gcagacatcg
     7261 aatccctgcg caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg
     7321 ccgaacaaga cctgcccctg cttgcagaac acatacagtc aaactgggaa accttcgccc
     7381 atatcgggca ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca
     7441 aacccgaatg accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag
     7501 ccgcccccgc cgatccgatt ctcggtttag gcgaagcgtt caaagccgaa acccgccccg
     7561 aaaaagtcaa cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccctcgtca
     7621 aagccgtcaa agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa aactacctca
     7681 ccatcgacgg cgttgccgac tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc
     7741 acgaaatcat cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat
     7801 tgcgtattgc ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca
     7861 atccgacttg gcccaaccac aacgccatcg ccaaagcggt cggtatccaa gacaaacctt
     7921 atcgttacta tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga
     7981 gccaagcgca aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca
     8041 tcgaccctac gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt
     8101 tgccgctgtt tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg
     8161 gcttgcgcgt gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa
     8221 acttcggtat gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa
     8281 cagcagcccg cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg
     8341 cttcacacgg tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat
     8401 ggattgccga actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg
     8461 agttgctcaa agccaaaggt gcaagccaag actttgattt cattatcgaa caaaacggta
     8521 tgttctcttt cagcggtctg acccccgaac aagttgaccg tttgaaaaac gagtttgcca
     8581 tttatgccgt ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt
     8641 atctgtgcga aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg
     8701 aaccttaacc agttcagacg gcatctgttt tttgttaaaa aacaataaac cataacaatt
     8761 tcaaacaagc taaaaaacat ctataaaatc aacccaatat gatgaactca aataaaaaat
     8821 acgaatatca tcattctcaa attcaaaatt tgaacgccac agtattatcc gaagccacaa
     8881 aaacctttcc gccgtcattc ccgcgcaggc gggaatccgg gaacccaatg ctgcaagaat
     8941 tgattagaaa caactgaaac cgagcaaact ggattcccgc ctgcgcggga atgacgaaat
     9001 aaggcgagta aaactgatga aacaaattga ttaaaaacaa taacttatat tattttattt
     9061 aaattttcga taaaatcaat tttccgtatt caatactgct ccaatcaatt cccgcgcacc
     9121 gtcgtccgcc aatttcttag cgacagcgcg tccgagcgcg tcggcatatt cggcaggggc
     9181 ttgcgcgtcc gcccgcaaca caaccgaacc gtcgggatga ccgaccaagc cgcgcaaggt
     9241 cagcaagccg ttttcttccg tgcaatatgc cgccagcggc acttggcagc ttccgcccaa
     9301 agcgcgcgcc aacgcgcgtt cagccgtaac gcaggcgtgt gtggtatcgt ggttcaacgg
     9361 ctttaagact tcatacaaat cttcgcgatg cgctgcaatt tcaataccca atgcaccttg
     9421 tccggcggca ggcagactgt cagattccga caaaatcatg cggatgcgtt cgtccaattc
     9481 cagacgctgc aaaccggcgg cggcaaggat aattgcgtcg tattcgccgt tatcgagttt
     9541 ggacaaacgg gtttgcacat tgccgcgcaa aggtttgata accaaatgcg gatagcgcgc
     9601 acgcaactgg gcttcgcggc gcaggctgga tgtaccgacg acagcgcctt tgggcatttc
     9661 ttccaaacgc gcgtattggt tggacacaaa cgcgtcaaac ggattggcgc gttcgccgat
     9721 ggcggcaagc gcgaaacctt caggcaaatc catcggcacg tccttaatcg aatgcaccgc
     9781 caaatcggcg cgcccgtcat ataaagcctg ttccaactct ttgacaaaca agcctttacc
     9841 gccgactttt gacaaagttt tgtccaaaat ctgatcgccg cgcgtggtca tgcccaaaat
     9901 ctcgacttcg caatcgggat acagagcctt cagacggcct tggatatgct ttgcctgcca
     9961 catagcaagc aggctttcgc ggctggcgat gacgagtttt ttcgggttca tgttcaatgc
    10021 gttcaaagaa tggaaacggc gtgaagtcta tcatattttg aacgtttctt cagacggcac
    10081 cgtcaggtct atgttatatt tacacccaca aaacacgaaa cccgccacaa tgaaacgcac
    10141 caaaaaatac cctttcttaa cgttgcagcg gcaacgtttc catttgaact ttgaaaacgc
    10201 ctcttccgcc gccggcatcc cagccgaacg cgatttctac cgctgggcgt ggtctgcctt
    10261 gaaaagcgga taccgccgcg ccgacatcag cctgattctt ctggacgaag aagaagcccg
    10321 agcctacaac cgcgactacc gcggcaaaga ttacgccacc aatgtattga gtttcgcgct
    10381 caacgaaggc gaaatcctgc cctgccaagt ttcggaaaaa ctgtacggcg atttgattat
    10441 ctgcccgcaa gtggttttga aagaagccgc cgaacagggc aaaacgcccg aacggcattt
    10501 tgcccacctg accatacacg gcgttttgca cctgatgggc tacgaccaca tcgaagacga
    10561 tgaagccgaa ataatggaag ccgaagaaat ccgcctgatg ctggcggcag gcttccccaa
    10621 cccctaccaa gaggacgaat attaaaatgg acggtacgca gtcgaaaccc aagtttttcg
    10681 aacgcctgat ctcccgactt gccggcgagc ccgactccgc cgaagacgta ttgaccctgc
    10741 tgcgccaagc gcacgagcag gaagtttttg atgcggatac gcttttaagg ttggaaaaag
    10801 tcctcgattt ttccgatttg gaagtgcgcg atgccatgat tacgcgcagc cgtatgaacg
    10861 ttttaaaaga aaacgacagc atcgaacgca tcaccgccta cgttatcgat accgcccatt
    10921 cgcgcttccc cgtcatcggt gaagacaaag acgaagtttt gggtattttg cacgccaaag
    10981 acctgctcaa atatatgttc aaccccgagc agttccacct caaatcgata ttgcgccctg
    11041 ccgtcttcgt ccccgaaggc aaatcgctga ccgccctttt aaaagagttc cgcgaacagc
    11101 gcaaccatat ggcgatcgtc atcgacgaat acggcggcac gtcgggtttg gtaacttttg
    11161 aagacatcat cgagcaaatc gtcggcgaca tcgaagatga gtttgacgaa gacgaaagcg
    11221 cggacaacat ccacgccgtt tcttccgaac gctggcgcat ccacgcggct accgaaatcg
    11281 aagacatcaa caccttcttc ggtacggaat acagcagcga agaagccgac accattggcg
    11341 gcctggtcat tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc attatcggca
    11401 gcctgcaatt taccgtcgca cgcgccgaca accgccgcct gcatacgctg atggcgaccc
    11461 gcgtgaagta agcagagcct gcccgcaccg ccgtttctgc acagtttagg atgacggtac
    11521 ggacgttttc tgtttcaatc ctccccatcc gccaaacata aaaatgccgt ctgaaaccgg
    11581 aaaacaggtt cagacggcat ttcgcttatc cgcttatttt ttgctcaaag tcagccccgt
    11641 ccagccgaat aacagggtta aagccaaact caaatagcag aaaaaggcat agggcagata
    11701 ttcccagacc ggcacaccca gcgcatggct gataaatacg ccgcacacgc tccacggaac
    11761 aagcgggttg atgaccgtac ccgcgtcttc gagcgtacgc gagaggttgc gggaatgtag
    11821 tccgagctta tcgtaaacgg gtttgaacgt ttcacccgac aacaaaatac tcaaatattg
    11881 ctcgccgatc aggaaattaa ccccgaccga agtcatggca acgctgaatg tggcacgtcc
    11941 ggcattcgtc aaaaagctgc ggacggcatc cagcagggaa ggaatcgcgc ccagcgcaaa
    12001 cagcagcccg cccaaactca tcccgagaat cacgatggtt tgcgtgaaaa acatactttc
    12061 caagccgccg cgcgaaatga gtttggcgat gtctttaaac gcttcgcctt cgagtttgta
    12121 accgccgtaa aaccacgcgc cgagctgacg cagatcgggc gtgctgtgca gatacgttac
    12181 ggcaacggca accatgacgg taaagagcat ggcgaccacg gcgttgacgc gcatcaatgc
    12241 caaaatgacc aacagcgcaa acggaatcag cgaatagccg tgcaccaatc ccgtggcttc
    12301 aagctggctg cggaaggatt cgacgctgtt caaatcctgc gcggcgacac tgggaagaag
    12361 ccacagcatc agtgccgcac tgatgagcca cgcgggaatg gtggtgtaca tcatattttt
    12421 gatgtgctca aacaggtcga tgccgacgat ggacgcggaa atgcccgtgg tgtcggaaag
    12481 cggggacatt ttgtcgccga aaaacgcacc ggaaacaatc gcgcccgccg tcatcgccat
    12541 atcggcatga aacgccgccg ccatccccat aaaggcaacg ccgacggtgg cgcaggtggt
    12601 caggctgctg ccgatggata cgccgatgac ggaacacagc gcgaaagcgg aaaaataaaa
    12661 ataagtcggg gaaatcagtc cgaaaccgta atacatcagc gtcggaatcg caccgctcat
    12721 catcagcgcg ctgaccatca gcccgatgaa gaaaaacagg taaatcgcgc ccataccctg
    12781 attcaacgcg cctatcatgc cctgctgcat atcgttgtat ttcaaaccgc gcgccaagcc
    12841 gtacaaaatc agcacgacga tggcggcaat aatggacata tgcggcagcc actccaatga
    12901 aatgatggta tagcccatcg cggcaatcag agcgacgacg acggcaagtg cctcaccgcg
    12961 cggcatatcg agtaaggatt tgaaagcgaa catgattctt tgcccgattt gatataaagt
    13021 taacggattc taacacacta taccgaatcc gcccgacgtt ttcagacggc attttagcgt
    13081 tttacaaaag ccggacagaa acaaaacggc aattttctca atttaaatta atttttttga
    13141 aatataattt tttatttttt tgtttgttga catataagac ttttatggat tgaagtatta
    13201 aaacctcggc acacacgccg caaacaccgg tacgcaaaaa tgccgtctga aagatgttca
    13261 gacgacattt ctcaagaaaa agattatttg tccgccctat atccgtacac cgcaaaaaac
    13321 acgatataca gataacagat gacggaaacg acaaacgaag acatcagggt gtaagtatct
    13381 gccacccagc cctgcacgac aggaacgacc gcaccgccga caatcgcggt acacagtaca
    13441 ccggaagccg cgttggtaaa ttttcccaat cctttggttg ccaaagagaa aatcgtcggg
    13501 aacataatcg agttgaaaaa accgatggca agcagcgacc acatcgccac atccgcattg
    13561 ccgctacccg tcgccatcgc gacggcaagc agtacgaccg cagccgatgc gttaaacgcc
    13621 aaataacggt tgggcgcgaa tttcgccatc accgccgaac cgaggaaacg tccgaccatc
    13681 gcgccgcccc aatagaacga caggtaatgc gcggcagaag catgatccag ccctttcaga
    13741 taacccaata cgttgaccat caacgaaccg atagacacct ccgcgccgac atagcagaaa
    13801 atgcctgccg taccgaacac gagatgtttg tattgccata cgctggtttt gccgtcgtgg
    13861 ttgtgcgcgc tttcctcggc ggcaattttg cgcgcgtcgg gcagccggat cattttcacg
    13921 aaaacggcaa ggataatcag cagccccgcc agtcccaaat agggaatctg tacggaagaa
    13981 atctgttccg ccttgctgac ggtttgggtt gcgtccgcca gaatcaggaa cgcgccgatt
    14041 tgcggcgcaa tggtcgtacc caaagcgtta aacgcctgaa ccagcgtcag tgttgccgat
    14101 tccttgccgg gtttcgccag cagggtaaca taaggattac cggcgacctg aagcagcgtt
    14161 acgccggaag ccaaaataaa caacgcgccc aaaaataccg cgtaagaatg gctacccgca
    14221 gcaggataaa acagcaggca tccgaccgcc gtcagcagaa agccgccgat aacgccgttt
    14281 ttgtagccga ctttgccgac aaaagccccc atcgggatgg acatcaccgc ataggcggta
    14341 aagaaacaga attggatcag catcgcctga acgtaagaca ggtcgaaaat ttctttcaaa
    14401 tgagggataa ggatgtcgtt catgcaggta ataaaaccca tcatgaagaa cagcgtggtc
    14461 aagacgacca atggggacgt atgattgttt tgtgattgcg cagacatgat ttgcctttcg
    14521 tggtgtcgga atattacccg tttcaagaaa atagaaattg acatttctta acggcaatgc
    14581 aaatcatact gatatttgaa cattttgcaa actttatcgg atgcaggcaa tgcgccgatc
    14641 tgaaattcaa gcagcagaaa ttaatatttt acagttgctt gcatagattt taatagattt
    14701 taaaaaattc cggcggactt gcaaaaaccg ttcccaataa aatgaaaatc cgccgccgta
    14761 tccggaaaat tcggaaatgc cgcatcgtgg catcaaaacc ggagcggaaa gaatgtcgta
    14821 tgcccgacaa aaaaggggca tacggctgtg ccgtattttt tgcaccgtcc tccgcccttc
    14881 ggcgggggcg tgaaaata
//
LOCUS       DAWKQJ010000038        14266 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000038 DAWKQJ010000000
VERSION     DAWKQJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.038"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(338..1114)
                     /gene="lpxA"
                     /locus_tag="WJL20_000856"
     CDS             complement(338..1114)
                     /gene="lpxA"
                     /locus_tag="WJL20_000856"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ7683027.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            complement(1201..1650)
                     /gene="fabZ"
                     /locus_tag="WJL20_000857"
     CDS             complement(1201..1650)
                     /gene="fabZ"
                     /locus_tag="WJL20_000857"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ7683028.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1684..2730)
                     /gene="lpxD"
                     /locus_tag="WJL20_000858"
     CDS             complement(1684..2730)
                     /gene="lpxD"
                     /locus_tag="WJL20_000858"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ7683029.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(2763..3263)
                     /locus_tag="WJL20_000859"
     CDS             complement(2763..3263)
                     /locus_tag="WJL20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ7683030.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVVAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3329..5722)
                     /gene="bamA"
                     /locus_tag="WJL20_000860"
     CDS             complement(3329..5722)
                     /gene="bamA"
                     /locus_tag="WJL20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ7683031.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5779..7119)
                     /gene="rseP"
                     /locus_tag="WJL20_000861"
     CDS             complement(5779..7119)
                     /gene="rseP"
                     /locus_tag="WJL20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ7683032.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7154..8389)
                     /locus_tag="WJL20_000862"
     CDS             complement(7154..8389)
                     /locus_tag="WJL20_000862"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ7683033.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8394..9191)
                     /locus_tag="WJL20_000863"
     CDS             complement(8394..9191)
                     /locus_tag="WJL20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ7683034.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9194..9940)
                     /locus_tag="WJL20_000864"
     CDS             complement(9194..9940)
                     /locus_tag="WJL20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ7683035.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9996..10553)
                     /gene="frr"
                     /locus_tag="WJL20_000865"
     CDS             complement(9996..10553)
                     /gene="frr"
                     /locus_tag="WJL20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ7683036.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10734..11861)
                     /locus_tag="WJL20_000866"
     CDS             complement(10734..11861)
                     /locus_tag="WJL20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ7683037.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11935..12010)
                     /locus_tag="WJL20_000867"
     tRNA            complement(11935..12010)
                     /locus_tag="WJL20_000867"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11975..11977),aa:Thr,seq:cgt)
     gene            12117..12311
                     /locus_tag="WJL20_000868"
     CDS             12117..12311
                     /locus_tag="WJL20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HEZ7683038.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGVAVGTVLFGLCSVCLRF"
     gene            12308..12589
                     /locus_tag="WJL20_000869"
     CDS             12308..12589
                     /locus_tag="WJL20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683039.1"
                     /translation="MNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAACAAAAVAWIFE
                     LLLGNAALGGLRGEAVSEYAFAVWLVAMLTLPKHLTRAPVQPVVFHRKK"
     gene            12685..13308
                     /gene="rsmG"
                     /locus_tag="WJL20_000870"
     CDS             12685..13308
                     /gene="rsmG"
                     /locus_tag="WJL20_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ7683040.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR"
     gene            13407..14180
                     /locus_tag="WJL20_000871"
     CDS             13407..14180
                     /locus_tag="WJL20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ7683041.1"
                     /translation="MSVNILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT         2695 a         3585 c         4519 g         3467 t
ORIGIN      
        1 tatattttta aaacaccact tttgtagtgt cctgtaattt cagcacagaa tatccaataa
       61 gacaatatgt tcttttgaaa aatacttttg gttttttcgc cgaaagcagg ccggttcaag
      121 ctgcggaaat tgtttgcaat tctttaaaag cagcggcgga ggtcacaatg aaatgtccga
      181 atagggatgt ggcgggcggc agaaatcatc aatgctgccg actgccatac ttctgaaatc
      241 tacaaaatga tgcatcgatc aaacaatata ccgctttaaa aaaaccgatg ccgtctgaaa
      301 cgctttcggg gtttcagacg gcatcaaaag ggtacggtca gcggatgatg ccgcgcgtcg
      361 attgtgtgaa aaagtctcgg aatacggcaa gctcggcttg ggtttcggcg cggcggagga
      421 tgtctgcctt ggcctcttca aacggaatgc cgcgatggta gagggttttg tacacgtctt
      481 tgacggcgga aatctgttct gcggtaaaac cgttgcggcg catgccttcg ctgttgagcc
      541 ccgccggttc ggcgcggtag cccgatgcca taaagtaggg cggtacgtct ttgtgtacgc
      601 ctgcggcgaa ggcggtcata gcgtagtcgc cgatgtggca gaattggaaa accagcgtgt
      661 agccgcccaa aacgacgtag tcgccgatgg taacgtgtcc ggcaagcgag gcgttgttgg
      721 cgaagatggt gtggttgccg accacgcagt cgtgcgcgag gtggcagtac gccataatcc
      781 agttgtcgtc gccgatacgg gtttcgccga tgccggttac cgtgcctaaa ttaaaggtgg
      841 tgaattcgcg gatggtgttg ccgttgccga taatcagctt ggtcggctcg tcgcggtatt
      901 ttttgtcctg cgggatttcg ccgaggctgg caaattggaa aatgcggttg ttttcgccga
      961 tgctggtgtg gccgttgatg acggcgtgcg ggccgatttc ggtattcgcg ccgatttgga
     1021 cgttggggcc gataacggtg tacgcgccga ctttgacgct ggagtcgagt tcggctttgg
     1081 ggtcgatgac ggcggtcggg tggatgaggg tcatgttttt cctttcctgt cgtgttgccg
     1141 cgaagatgcg cgacggcaac aggtcgtctg aaaactttca gacgaccttt ttctgaacac
     1201 tcaaaccacg cgtttggcac acatgatgat ggcttcgacg gcgacttgcc cgtccacttt
     1261 ggcaacggcg ttgaatttgc cgatgccgcg ccggctggtc agcagctcga cttcaaagac
     1321 gagttggtcg ccggggatga cttggcgttt gaaacgggct tcgtctatgc cggcgaagaa
     1381 gaagaattcg ttttctttgc gcccgccttc gctcaaaatc gccaacgtgc cgcacgcctg
     1441 cgccatcgct tcgatgatga gtacgccggg catcacgggc aggtcgggga aatggccttg
     1501 gaactggggt tcgtttatgg tgacgttttt aatcgcggtc agggttttca tcggctcgaa
     1561 ggcggtgatg cggtcgagct ggagaaacgg atagcggtgg gggatgagtt tttggatgtc
     1621 tttggcttcg atggggagtt gaacgtccat gtctgtcgta ttccttgaat aaagtcggtt
     1681 tggttatttg ctgtcttgac cggcatctga aagctgctgc tccagtgttt tgagccgttt
     1741 gttcatttcg cttaagcggt ggatgtaaac agcgttgcgc gcccattctt tatgggtgga
     1801 catcgggaag atgccggcga ggtgtttgcc gctttcggta atgctgtggg tgacggacgt
     1861 gccgccgccg atggtggttt tgtcggcgat ttcgatgtgt ccgaccgtac cgacgccgcc
     1921 gccgatgatg cagtagctgc ctatggttac gctgcctgag atgccggttt tggcggcgat
     1981 gacggtgtgc gaaccgattt tgcagttgtg tccgatttgg acttggttgt cgattttggt
     2041 gccgttgccg acggtggtgt cgctcatcgc gccgcggtcg atgttggtgt tcgagccgat
     2101 ttctacgtcg tcgcccagcg ttaccgcgcc ggtttgcggg attttaaacc atgaatcgcc
     2161 ggcaaaagcg agtccgaaac cgtccgcgcc gatgaccgcg ccgctgtgga tttcgacgcg
     2221 tctgcccagt gtgcagccgt aataaacgac ggcgttggga tgcaggacga cttcgtcgcc
     2281 cagtttgcaa tcgtgttgga cgacggcgtt tgccaagatg cggcagcctt cgccgagcac
     2341 ggtgtttgcg ccgatgtagg cgttcgcgcc gatttcgcag cttgcgggaa cggtggcact
     2401 cggttcgacg acggcggtcg gatggatgcc gccgcgcgct ttgacgacgg gtgaaaacag
     2461 gcgggcgact ttggcgaaat agagataggg gtcgtcggcg acaatcaggt tgcgcccttc
     2521 aaatccgtct gccgctttgg cggaaacgat gaccgcgccc gcgctgctgt cgtggacttc
     2581 ggctttgtat ttcggattgg caaggaagct gatgtgttcc gcctgcgcgt ctgcgagcgg
     2641 gcgcacggcg gtaacggaaa tgtcctcgcc gcgccattcg ccgccgagcc gcgcggtgat
     2701 ttgggacagg gtgtaggtgg ccggaatcat ggttttcctg ttcggtatgc cgtctgaaag
     2761 ggtcagcggg cgttcatttc tttaatgacg ctgtcggtaa cgtcgtattg ggtgttgacg
     2821 taaatcacgt tctgcaaaat gacatcgtaa ccttcctgtt tggcgatttt gacgatgacg
     2881 cggttggcgt tttgctggag ggaggcaaac tcttcgttgc ggcggaggtt gtagtcttct
     2941 tcaaactgcg cctgtttttt gcggaacgct acgaccagcc cgcgccattt ttcttcggct
     3001 tgcgcctttt ttgcgtttct gagtttgcct tcggcaagct gcctttccaa atccagacct
     3061 tcgcgttgca gtttttgcaa ttcgtcctga cgggcggaaa attcgctgtc cagcgttttt
     3121 tgaatcttgc gcgcctgctt ggattcgagg tagatgcgct cggtgttgat aaagccgatt
     3181 ttttggaagg tgtcggcgtg cgcgcctgcg gtgcagcaca aaccgatcag agccgcggca
     3241 aacgcgcggg tcaaacgggt catggtaaaa ctccttcgaa tgttgccgcg aaatgccgtc
     3301 tgaagggctt cagacggcat ttgcgggatt agaacgtcgt gccgagttgg aattggaagc
     3361 gttggatttc gtcttccggt tttttcttca gcgggtaggc gtagctgaat ttcatcgggc
     3421 ctaaaggcga gagccaggta accgcgccgc cggcggaata gcgcaattcg ttggtaaagg
     3481 tggatttatg ggtattgccg gcgccgtaaa tgttttgaac cctgccgccg gtcgcggaac
     3541 tgctgttgtc gtcgtaggtt ttgccgtccc acacgctgcc tgcgtcggca aacaggctca
     3601 ggcggacggt gcgcgcgtct ttcgcgccgg gcatcgggaa gagcagctcg gcggagacgt
     3661 tggctttttt gttgccgccg tagctgattt tttcgccgta ttcgtcatag actttcggac
     3721 cgagtgtgcc gctttcgtaa cctcgtaccg aacccaggcc gccgccgtag aagttttcaa
     3781 agaaggggat ttctttggtt ttgccgtagc cgttggcata gccgatttcg ccgccaagca
     3841 tcagtgtgaa ggttttgctc agggggaaga accaggtttg gttgtgggtg gcggagtagt
     3901 attgcagttt gctgccgggc agggcgattt cggcgttcac gcccgtcagg tagccgcgcg
     3961 tcggccataa cgcgctgtcg gttttgttgc gcccccagcc gacggtacct ttgtacagcc
     4021 agcctttgaa gctgccgtct gtgccgtcgg ttttgccgta tttcttgata aagtcggcat
     4081 agtgtttggg cgctttgttg taggtgttga cggtcaggtg ttctgccacc aaaccgaaat
     4141 tcacgcggtc gtattcggta acaggcacgc tcatgcggat gcctgcgcct gccgtggtgg
     4201 ttttatattg tttgatgctg gtcgatgctt tgcgcgggtc gaaggctttt ccgtaaacat
     4261 cgtagcccag gctgaccccg tctgccgtga agtacgggtc ggtaaacgac agcgagccgt
     4321 tgagcgtggt tttgcttcgt gaggcgcgca gggcggccga cttgcccgta ccgaacaggt
     4381 tgtcttggga aacgcctgcg gacatgacca ggccggtatc ctgtacccag cccgcgctca
     4441 agtcgagcga gccggtggaa cgttcggtca ggctcatgtt caaatcgact ttgtcgggtg
     4501 tgccggcaag cgggacggca tcaaactgta cgttgtcgaa gtagcccaaa agctcgacgc
     4561 gctctttgga gcgttgcagc ttggaggtgt cgtaaggcgc ggattccatt tggcgcaatt
     4621 cgcggcgcac gacttcgtcg cgggttttgt tgttgccggt gatgtggatt tcgttgacgt
     4681 agattttccg gcccggttcg atgtgcagga cgaaatcgac ggttttggtt tcggcgtttg
     4741 gcagcggctg tacgctgatt tcgctgtatg cgtagcctgc cgagcccatg cggttctgaa
     4801 tctcacccaa aacggcggtc atctgctggc gttcgtacca tttgccgggc ttcatggtca
     4861 gcagtttttc cagttcggct ttggggactt cgttggtgtc gccttcgatg gagactttgc
     4921 cccaacggaa acgttcgcct tcgtggacgg tgattttgat ggtctgcttg gttttgtctt
     4981 cgttggtttg gatgtcggta tcgaggatgc ggaaatcgaa gtagccgttg ttttggtaga
     5041 agtcggttac tttttccatg tcttgggcaa atttctgctc gttgaattgg ttgcttcgtg
     5101 tcagccatgt ccaaatgccg ccttcggtca gcgacatctg ccgcatcagt ttgcggtcgg
     5161 aatagacttg gttgccttca aattcgatgt cggtgatttt ggcggatttg ccctcgtcaa
     5221 tcgtgatgtc gatgtcgacg cggttgcggg cgagtttggt tactttgggc gtgatttgga
     5281 tattgagttt gccgcgcccg aggtattctt ctttcaggcc ggcgactgcc tgattgagtg
     5341 tcgcctgatt aaagtattgc gactgcgcca gcccgaacga ttcgagattt ttcttaatgg
     5401 cgtcgttttg cagcattttt gcgccggtga tgttgagcga gccgatggtg gggcgttcga
     5461 tgacggtcag caggagctgc ccgtccgcag tctcgacgcg tacgtcgtca aagaaaccgg
     5521 tggcgtacag gcttttgatg atggcactgc cgtgtgtgtc gttgtaggtg tcgccgactt
     5581 tgacgggcag gtagttgaat acggtactcg gctcggtacg ctgcaagcct tcgacgcgga
     5641 tgtcttggat ggtgaagtcg gcaagtgcca aaggcgatat gcccaagacc atcagtgcgg
     5701 aagcaatctg tttcagtttc attgtcagtt ccttgtggtg cggaatgcgg tttcagacgg
     5761 cattccgaaa cgtaaaatct aaccgagcag ccgggtaacg tcgttgaaga aggcgaccgc
     5821 catcatcagc atcatgaggg caagcccgaa gcgcaaaccg atgttttgga cgcgttcgcc
     5881 caaaggtttg ccgcgtatcc attcggcagt ataaaacacg aggtggccgc cgtccaaaac
     5941 ggggacgggc agtaggttca gcacgccgag gctgatgctg accagtgcca aaaattccaa
     6001 ataactttgc aagccgagtt cggcggactg tccggcaatg tcggcaatgg tcagcggccc
     6061 ggaaatatgg ctgacggagg cgttgccgct gattagtttg ccgaaaaatt tgagggttgt
     6121 ccacgagtgg gaaacggttt tttcccagcc catgccgaat gcgcggataa cagacggacg
     6181 gtagctgcgg cggatttgcg cgtcccacgc cctgtccggc tgcggacgga ggccgacgcg
     6241 cccgatcagg gtgtggtcgg actgttcgac agtatcgggg cggatgtcgg cggtatgggt
     6301 ttgtccggcg cgttcgtagt tcagggtgat ttttttgccg gggctttggc gggtcaggtt
     6361 tgcccattct tgccatgagg cgatgggttt gccgtcggcg gcagtcagcc tgtcgcccgg
     6421 tttcaggcct gctttttcgg cggggctgcc tttttccacg ccgccggcaa cggttgtgat
     6481 tttaaagggc atcagtccga tgtagccttg gttttttgcg attttaccgg cttccggcgt
     6541 gcctgcggca tcgatggtgc ggacggtttg cgcgcccgat gccgtctgaa cgccgacggc
     6601 gactttgccg gcttcgaggt tgaggacgat ttcggtttgc gcgctgcccc aatctgcaac
     6661 gggtgtgccg ttgacggatt gtattttgtc gccgctttgg aagccggcgc gggcggcaat
     6721 ggtgtcaggt tcgactgtgc cgacgtaggg gcgcagttcg gttacgccga aggaaaagct
     6781 cagtccgtac agcaaaaccg ccagtgcgag gttggtcagt gggccggcgg cgacgatggc
     6841 gatgcgcttg gcggggtgtt gtttgtcaaa agcgtagggt aaatcggctt ctgatacttc
     6901 gccttcgcgc gtgtcgacca ttttgacgta accgcccaac ggaatcgggg cgaggcacca
     6961 ttcggtgtcg ccgcgctttc gggtgaaaaa cggtttgccg aagccgacgg aaaagcgtac
     7021 gactttgaca ccgcacaatc tggcaacgat gtagtgtccg aactcgtgca ggctgaccaa
     7081 aatcaggatg gcgaagataa aagctagaag ggtgtgcaaa tggttttcct ttgataacgg
     7141 tgttcggacg gcatcagcgc agtgtgccga taaatgcccg cgcttgtgcg cgtgtccggg
     7201 catcttgcgc caagagcccc cctatgtcac ctatgccgtc tgaaaagtct tgtgaaagac
     7261 aatgggcgac ggttttggca atgtcggtaa acttaatctg tccgtccaaa aaggcggcga
     7321 cggcggcttc gttggcggcg ttcaatacgc agggcgcgac cccgcccgcg ttcatggctt
     7381 cataggcgag cttcaggcag gggaagcggt caaagtcggg cttttggaag gtcagcgcgg
     7441 acaatgcgtc gaaatcaagg tcgccgacac ccgaatcgat gcgctcgggc aagcccaaac
     7501 aataggcgat gggcgttcgc atatcgggat tgcccagttg tgccagcacg gagccgtcgc
     7561 ggtagcgcac catgctgtgt atcacggatt gcggatggat gacgacttcg agtttgtcgg
     7621 gcggacagtt gaacagccaa tgcgcttcaa tcagctccaa acctttgttc atcatggtgg
     7681 cggaatcgac ggagattttg cgccccatac gccaattggg gtgtttgacc gcttgggcgg
     7741 gcgtaatgcg gtcgaacgtg cctaaatcgg tcgttaaaaa cgggccgccg gaagcggtca
     7801 ggataatcga acggatgccg tgttcgttca gacggccggc gtaatcgcgc ggcaaaactt
     7861 ggaaaacggc gttgtgttcg ctgtcgacgg gcagcactgc cgcgccgttt gcacgggcgg
     7921 tttccataaa caacgcgccg gaaaccacca gcgtctcttt gttcgccaga taaatggttt
     7981 tgcctttttg cgccgctgcg agcgcggaag gcagccccac cgccccgacg atggcgcaca
     8041 tgacaccgct gacttcgtcg gcagaggcaa cgtcaaccaa tgcctgcgcg ccgtgtaaaa
     8101 cctgcgtcgc cgtgccgtcg cgtttcaaca gggcttcaag ccgggcggcg tattcggcat
     8161 cggcaacgac ggcatattcg ggttggaacg tttgacattg agccgccagt ttctcgacct
     8221 gcttatgccc tgccagcgcg aatacgcgga atttttcggg gtggcgggag acaacgtcca
     8281 gcgtgctttc gcctatgctg ccggtactgc ctaatatggt caggacttgt ggtgtcataa
     8341 tggggataac tttataccgg atgccgtctg aagcgttttc agacggcata gaatcaattt
     8401 aaaaccgaca tcatcgccgc atagacgctg ataacggcaa tcaggctgtc ggtacggtcg
     8461 aacacgccgc cgtgtccggg cagcagcttg ctgctgtctt tgatgcctgc cgcgcgcttg
     8521 agccagcttt ccaaaaggtc gccgcatacg ctgacaacgg tcaacaccaa accgattaac
     8581 acggtatcga accagcctgt atcgaatgcc agccagccgg cacttcgtac ggcggtcatg
     8641 tacacggcca cgcaaaccgc gccgccgatt gcgccttccc agcttttgcc ggggctgatt
     8701 gccggtgcga ttttgtgttt gccgaacgcc ttgccgctga aatacgcgca aatatcggca
     8761 acccacacca aacccatcac ggcgagcagc ggcagggcat catcgggatg cgggcgcagg
     8821 gatacgagcg cgaaccaaaa cggcatgacc agaagccagc cgacggcata aacctgccaa
     8881 ccgccgttga gcctccattt gaatctcaac cataaaggca taacggcgag ccaaaatgcc
     8941 aaaacaacat accaaaccaa attaggcagc atccagccgc ccgcataggc aaccacgccg
     9001 aaaaccaagg ttgcggcgag gtaatggttg gttttaattt tgcacaaacc gcccatacgg
     9061 gcatattccc acaaggcaat cagggcaatc agtccgcaaa atgcagccca caaccattgc
     9121 ggcgcgtaaa acagcatgcc cagcatcagc ggcagcagcc acatggcggt cattacccgt
     9181 tgtttcagca tattcagttc ctttgctgtt cgataggcag ttgctcggag gtgcgtccga
     9241 accgccgttc gcgtttttgg aacgaagcga cggcatcgtc caaagccttg ccgtcaaaat
     9301 cgggccacaa aatatcggtg aaatacagtt cggcataagc catctgccag agcaggaaat
     9361 tactgatgcg cgtttcgccg ccggtgcgga tgaacaaatc cggctcgggc gcgtctccca
     9421 gcatcaggta ttttgccagc gtgtcttccg taatctcgga tacgccttcg gcaatcagtt
     9481 tgtttgccgc ctgcaaaata tcccaacgtc cgccgtaatc ggcggcaatg ctcagggtca
     9541 ggccggtatt gtttgccgtc aacgcctccg cctcttcgat gccttgcaga atctgccgat
     9601 taaagcgttc gcggctgccc agtattttca ggcgcatatt gttttcgtgc aggcggcgta
     9661 cctgtttttg caaagcctgt aaaaacagcc ccatcaggaa cgaaacttcg tcttcggggc
     9721 ggcgccagtt ttcggtcgaa aaggcaaata cggtcagata ttgcacgccc aatttggcgc
     9781 aatgcctcac catattttcc aatgcgtcca aaccgcgttt gtgtcccatt atgcgcggga
     9841 ggaaacgttt tttcgcccaa cggccgttgc cgtccataat cacggcgata tgcttgggaa
     9901 tggcggtgtg ttccaaaacg gcctgcgtgc tgcttttcat gtctgccttt cgcggttcgg
     9961 cattcaaatg ccgtctgaac gccgaaccgt gcaggttaaa ttgccatcaa atcttcttct
    10021 ttggcagtca ggagtttgtc ggcttcggta atgtatttgt cggtcagttt ttgaaccgct
    10081 tcttcgccgc gacgtgcctc gtcttcggaa atttctttgt ctttgaggag ttttttgatg
    10141 tggtcgttgg catcgcggcg cacgttgcgg atagagacgc ggccttcttc cgcttcgccg
    10201 cgtacgactt taatcaggtc tttgcggcgt tcctcggtca gcatgggcat cggcacgcgg
    10261 atcagatcgc cgacagctgc cgggttcagt cccaagtttg aatcgcggat ggctttctcg
    10321 actttggccg ccatattgcc ctcaaacggt ttcacgccga tggtgcgcgc gtccagaagc
    10381 gttacgttgg caacttggct gacgggaacc atgctgcccc agtattcgac ttccacttgg
    10441 tcgagcagac cggtatgcgc gcggccggta cgcactttcg ccagattttc tttcagtact
    10501 tcgaccgaac gctgcatttt gccttcggct gttttttgaa tatcgttaat catattgttc
    10561 tttcggtggg ataaggtggg cgggcagccg tctgaaacgc gcttcaaacc tttcagacgg
    10621 catgaaaact gttaactgcg aatagtaccg ttattcgggc atgacgacaa ggcaggagga
    10681 ttggagatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccga ccgtcagccg
    10741 tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc atccagccat
    10801 ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg catattggtg
    10861 ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc ttccagttcg
    10921 gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat aagggcgacg
    10981 gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg gatttcgctg
    11041 ccgttgagtt tgggagattg cagcttggcg gcagtggtca ggagcagctc ggtggtgttg
    11101 acaattttac ggtgggcgtg ctgcatggct tccatcatgg cggggctgat gcggctttcg
    11161 cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat ttggcgcatt
    11221 ttcgccatgt tctcttcgag gcgttcgcgc gtcatacgcc tgccgttgct gatttcggca
    11281 atcattttgc tgcagtcggt caggttgtcg gcaagcatga aacgccacat cagtgtggat
    11341 ttcagcggca gcagtttggc ggcggcaatg gcgatggccg cgccgatgag gacgttcatg
    11401 gcgcgcatca ggccgctgtc gaaccattcg ctgccgttgt cgccgatgag catgcacatc
    11461 gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca gccggccagt
    11521 gcgcttgccg tgccgacggt gaggtagaag aggaggttgc cgtggaaata atgctggttc
    11581 agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg tgccgagcat acgttccacc
    11641 gccttggagt aaatcgcccc ttgaaactgg agcatgccga ggacgacgaa gacggtcatg
    11701 cctatccatt cgccgtgttg gaggtggagc agccgggcga gtgcggtggc gaacaggacg
    11761 gccccgccga gccgcacggc gtgtatgagg cggcggtggc ggtagcgttc gtaggagttg
    11821 agccagcggc tgacgaggcg gttgcgttgc gaggtgttca tatcggttgc cgtctgaagc
    11881 ggaaatgtga aaaagcacag gcttcccgag gaagggaggg tctgtgcttg gtattggtgc
    11941 cggagaaggg aatcgaaccc ccgaccttcg cgttacgaat gcgctgctct accgactgag
    12001 ctacaccggc gttttttcgt tatgatatat atgaacggtt gtttgtgcaa cttttctggc
    12061 gggcggcaag gcagtgcgcg gtataatgcg gtctgcttcg gaagaggggg acggcgatgt
    12121 ttgtgaacga gaaagatcct tatgcggctc tgtttgcggg gctggtgttt ttgacgttgc
    12181 cgtttgcgtt ggcggtgcat gatgcctttg cgcttgcgtt cggacgggca gggctgctgg
    12241 tgtcggtgtc ggccggcgtg gcggttggga cggtactgtt tggtttgtgt tcggtgtgtt
    12301 tgcgtttttg aacctggtcg tgtcggcagg cctgacgaag ctggcgtaca aaaagatgat
    12361 gcggcggcat tcgcgttacg cgctgtttct gtcgggcgtg gcggcttgcg cggcagcagc
    12421 ggtggcttgg attttcgagc tgttgttggg caatgccgcc ttgggcgggc tgcgcgggga
    12481 ggcggtgtcg gaatatgcgt ttgccgtgtg gctggtggcg atgctgacgc tgcccaaaca
    12541 cctgacgcgc gcgccggtgc agccggtggt gtttcacagg aaaaaatagg acggaacggg
    12601 ggatgccgtc tgaaacccga cacgcggttt cagacggcat gtttttccgt taacattacg
    12661 cctgaatatg gacaggaagc agatatggaa cgcaaagaac gcctgcgtgc aggcattgcc
    12721 gcgatggggc tggatatttc ggaaacggcg caggacaggc ttttggccta tgtggatttg
    12781 ttgaaaaagt ggaacaaaac ctacaatctg accgccctgc gcgacgagga aaaaatgatt
    12841 gtccatcatc ttttggacag cctgacgctg ctgccccata tcgagggtgt gcaaacgatg
    12901 ctggatgtcg gttcgggcgg cggtcagccc ggcattccgg cggcggtgtg ccgtccggat
    12961 gtgcagataa cccttttgga tgcgaatacg aagaaaacgg cttttttaca gcaggcggtt
    13021 atcgagttgg gattggacaa tgtgcgcgtg gtatccggac gcgtggaggc ggtttcggac
    13081 gtgcgtgccg acgtggttac cagccgtgcg tttgcagaac tggcggattt tgtgtcgtgg
    13141 acggtgcatc tgttgaaaga cggcggatac tgggcggcga tgaagggcgt gtatccgcag
    13201 ggggaaatcg accgcctgcc gcaggatgtg tgcgtcgaaa aagtccaaag gcttgacgtg
    13261 ccgggcttgg atgcggaacg ccatatcgcc atactccgca agcgttgagc gcgcttcaga
    13321 cggcatgaat accttttttg tgcggataaa ggtaaaattc cgcactgttt ttcttttttc
    13381 aacatcagac gggacacggg cgggacatga gtgtgaacat ccttgccatc gccaatcaga
    13441 agggcggtgt gggcaaaacg acgacgacgg taaatttggc ggcttcgctg gcatcgcgcg
    13501 gcaaacgcgt gctggtggtc gatttggatc cgcagggcaa tgcgacgacg ggcagcggca
    13561 tcgacaaggc gagtttgcag tccggcgttt atcaggtctt attgggcgat gcggacgtgc
    13621 agtcggcggc ggtacgcagc aaagagggcg gatacgctgt gttgggcgcg aaccgcgcgc
    13681 tggccggtgc ggaaatcgag ttggtacagg aaatcgcccg ggaagtgcgt ttgaaaaacg
    13741 cgctcaaggc agtggcggaa gattacgact ttatcctgat cgactgcccg ccttcgctga
    13801 cgctgttgac gcttaacggc ttggtggcgg caggcggcgt gattgtgccg atgttgtgcg
    13861 aatattacgc gcttgagggg atttccgatt tgattgcgac cgtgcgcaaa atccgtcagg
    13921 cggtcaatcc cgatttggac atcacgggca tcgtgcgcac gatgtacgac agccgcagca
    13981 ggctggttgc cgaagtcagc gaacagttgc gcagccattt cggggatttg ctttttgaaa
    14041 ccgtcatccc gcgcaatatc cgccttgcgg aagccccgag ccacggtatg ccggttatgg
    14101 cttacgacgc gcaggcaaag ggtaccaagg cgtatcttgc cttggcggac gagctgatgg
    14161 cgagggtgtc ggggaaatag gtcaatccaa atcgggctgc ccgtgccttt atgctgtttg
    14221 gccgagtgcg ttaatatggc ggattaaaat aaaaatactt atatcg
//
LOCUS       DAWKQJ010000039        13923 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000039 DAWKQJ010000000
VERSION     DAWKQJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.039"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(103..1428)
                     /locus_tag="WJL20_000872"
     CDS             complement(103..1428)
                     /locus_tag="WJL20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ7683042.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1642..2481
                     /locus_tag="WJL20_000873"
     CDS             1642..2481
                     /locus_tag="WJL20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ7683043.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2478..3644
                     /locus_tag="WJL20_000874"
     CDS             2478..3644
                     /locus_tag="WJL20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ7683044.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3908..5173
                     /gene="efeB"
                     /locus_tag="WJL20_000875"
     CDS             3908..5173
                     /gene="efeB"
                     /locus_tag="WJL20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ7683045.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5243..5572)
                     /locus_tag="WJL20_000876"
     CDS             complement(5243..5572)
                     /locus_tag="WJL20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ7683046.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5647..7017)
                     /gene="glmU"
                     /locus_tag="WJL20_000877"
     CDS             complement(5647..7017)
                     /gene="glmU"
                     /locus_tag="WJL20_000877"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ7683047.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLKTLLEAAGNKVGLLTDVPADPTGLGRIIRDSNGSVTAIVEEKDADTA
                     QKAVKETNTGILVLPNAKLEKWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRT
                     SYLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(7072..7344)
                     /locus_tag="WJL20_000878"
     CDS             complement(7072..7344)
                     /locus_tag="WJL20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ7683048.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7337..8050)
                     /locus_tag="WJL20_000879"
     CDS             complement(7337..8050)
                     /locus_tag="WJL20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ7683049.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            8109..9110
                     /locus_tag="WJL20_000880"
     CDS             8109..9110
                     /locus_tag="WJL20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7683050.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9204..10052
                     /locus_tag="WJL20_000881"
     CDS             9204..10052
                     /locus_tag="WJL20_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7683051.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10079..10564
                     /locus_tag="WJL20_000882"
     CDS             10079..10564
                     /locus_tag="WJL20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ7683052.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10666..12234)
                     /gene="msrAB"
                     /locus_tag="WJL20_000883"
     CDS             complement(10666..12234)
                     /gene="msrAB"
                     /locus_tag="WJL20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ7683053.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12379..13644
                     /gene="ftsY"
                     /locus_tag="WJL20_000884"
     CDS             12379..13644
                     /gene="ftsY"
                     /locus_tag="WJL20_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ7683054.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2990 a         3566 c         4179 g         3188 t
ORIGIN      
        1 cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa aacttcgcct taatgcgccc
       61 gattgactag ggatgggctt cagatggcat tgttttgccg gtttacgggc ggtattcggg
      121 cttcataccg ttgggtagga gctgccagac gtaacccgtg gttttctgtt tgccggcaag
      181 ttcgccggct tcgtcgccgt atccccaaaa ataatccacg cgcaccgcgc ctttaatcgc
      241 gctgccggta tcctgcgcca taatcaggcg gttgagggct ttgcgggtaa ccggatgggc
      301 ggtggcgaca aataagggcg cgcccaaggt aatgtagtgc cggtcgactg cgccggcata
      361 ttcccccatc aacggcgtgc ccagtgcgcc gacgggaccg tcattgctgc ttccggcaag
      421 ctcgcggaaa aagatatagc tggggttttg acccaaaact tcggcgaggc gttgcggatt
      481 ttgccgcata taggctttga tgccctgcat ggaggtctgc ccgagcttga ggtagccttt
      541 gtccgccata tagcgtccga tggaaacgta gggatgttcg tttttgtcgg catagccgat
      601 gcggatgtat ttgccggacg gggttttcag acggcccgag ccttggatgt gcataaaaaa
      661 aagttcgaca gggtcttcgg cgtaaccgag tatcggtgct ttgccgtcaa gcgcgccgcc
      721 gttgatttgg ttgcgcgtgt ggtaggggag gaagcggctt ccttcaaacc tgcctttgat
      781 tgccgttgtg cgcgcagtga tggggaattg ggagaggtcg gcggtatgtg tgccgccggt
      841 attgtcgatt gtgccgctgt tttttcccgt ctgcctgatg cggacaaggg cttttccgct
      901 ccgcaaaccg gcaggcaggg ggacggagat aaaatcgtcg ggaataccgt aaatcgggaa
      961 gcgggcttgt gccgtccgcc tgtcgtcgcc cttcagcacc ggctcgtaat agccggtaac
     1021 cgtaccggca aggcttccgt tgcctgcaac ctgccacggc gtgaaatagc gttcaaaaaa
     1081 ctgttttgcc tgaaaggaat ggacgggggt ttgaaaggct tgggcgcaca catcctgcca
     1141 gccttggcgg tttttcaaat tggcgcagcc gaggcggaag gattgcaggc ttttggcgaa
     1201 atcctgcgcc gcccagtggg gcagggacag gtgcggcaca acggtataaa cggccccgcc
     1261 gccgccgacc gtcgttccgg cggggtcggg gatgccgacc ggccggtccg ggccgttgat
     1321 gacggatgtg tcgggttgcg gaaaggtttg gatgctcttg ctttggcagg cggcgaggat
     1381 ggcggcggcg atgccgcaca gggcggcgcg gaacaggtat tttttcataa tggacaatgt
     1441 tgccggcagt aataaaaaaa aagatggttt cgggcggcgt tgcggcagcc gtggagaggg
     1501 gattttaaca cagggcgcag ctgcagcctg cggaactttc cgccgcgcgg caatgcagat
     1561 aaaaataact tgcatttgta tttacaagca atgaaaatat tccgataata tattattcat
     1621 catccttgtt cgttcgcgtt tatgctggtc gcttttttaa ttatgttgcg cgagggtatc
     1681 gaagccgcgc tcatcgtcgg catcgttgcc ggttttctga aacagtccgg acattccaaa
     1741 ctgatgccta aggtctggtt cggggtcgtc cttgcttctt tgatgtgttt gggcatcgga
     1801 tacggcatcc attcggcaac gggcgagatt ccccagaagc agcaggagtt cgtcgtcggc
     1861 attatcggtt tggttgctgt cgcgatgctg acttatatga ttttatggat gaaaaaggcg
     1921 gcgcgttcga tgaagcggca gcttcaggat tctgtacagg cggctttgaa ccgtggcagc
     1981 ggtcaaggat gggccttggt cggtatggcg tttcttgccg tggcgcgcga aggtttggag
     2041 agcgtttttt tcctgcttgc cgtattcaaa cagagcccga cgtggcagat gccggccggt
     2101 gcggtagcgg gggttttggc tgccgccgtg attggcgcgt tgatttatca gggtgggatg
     2161 cgcctgaatc tggctaagtt tttccgttgg acgggggcgt ttttgattgt cgttgccgcc
     2221 ggcctgcttg ccggctcgct gcgcgcgctg cacgaagcag gcatttggaa cgcgcttcag
     2281 gacattgtgt tcgactcatc aaaatatttg cacgaagaca gtccgttagg tgtgctgctc
     2341 ggcggatttt tcggctatac cgaccatccg acgcagggcg aggccttggt ttggctgctg
     2401 taccttattc ccgtcatgac ttggtttttg tgcggcagca ggccgtctga aactttaacc
     2461 cgtaaagagg agctgaaatg agaaaattca atttgaccgc attgtccgtg atgcttgcct
     2521 taggtttgac cgcgtgccag ccgccggagg cggagaaagc tgcgccggca gcgtccggtg
     2581 aggcgcaaac cgccaacgag ggcggttcgg tcagtatcgc cgtcaacgac aatgcctgcg
     2641 aaccgatgga actgaccgtg ccgagcggac aggttgtgtt caatattaaa aacaacagcg
     2701 gccgcaagct cgaatgggaa atcctgaaag gcgtgatggt ggtggacgag cgcgaaaaca
     2761 tcgcccccgg actttccgat aaaatgaccg tcaccctgtt gccgggcgaa tacgaaatga
     2821 cttgcggtct tttgaccaat ccgcgcggca agctggtggt aaccgacagc ggctttaaag
     2881 acaccgccaa cgaagcggat ttggaaaaac tgtcccaacc gctcgccgac tataaagcct
     2941 acgttcaagg cgaggttaaa gagctggtgg cgaaaaccaa aacctttacc gaagccgtca
     3001 aagcaggcga cattgaaaag gcgaaatccc tgtttgccga cacccgcgtc cattacgaac
     3061 gcatcgaacc gattgccgag cttttcagcg aactcgaccc cgtcatcgat gcgcgtgaag
     3121 acgacttcaa agacggcgcg aaagatgccg gatttaccgg ctttcaccgt atcgaatacg
     3181 ccctttgggt ggaaaaagac gtgtccggcg tgaaggaaat tgcagcgaaa ctgatgaccg
     3241 atgtcgaagc cctgcaaaaa gaaatcgacg cattggcgtt tcctccgggc aaggtggtcg
     3301 gcggcgcatc cgaactgatt gaagaagtgg cgggcagtaa aatcagcggc gaagaagacc
     3361 ggtacagcca caccgatttg agcgatttcc aggccaatgt ggacggttcg aaaaaaatcg
     3421 tcgatttgtt ccgtccgctg atcgaggcta aaaacaaagc cttgttggaa aaaaccgata
     3481 ccaacttcaa acaggtcaac gaaattctgg cgaaataccg gactaaagac ggttttgaaa
     3541 cctacgacaa gctgggcgaa gccgaccgca aagcgttaca ggcctctatt aacgcgcttg
     3601 ccgaagacct tgcccaactt cgcggcatac tcggcttgaa ataagccgca agcgttcaga
     3661 cggtatttgg cggcagatac cgtctgaagt tttatagtgg attaacaaaa accagtacgg
     3721 cattgcctcg ccttgccttg ccgtactatt tatactgtct gcggcttcgt cgccttgtcc
     3781 tgatttttgt taatccacta tatccgccat atattgcagg gcaggatttc aacctgccgc
     3841 tatcggttaa tggaaaaacg gcgtgcaggg atacccatcc tgctgcacgg atattgaagg
     3901 aaacaccatg agcaaaaacc aacccgcaca accgaccagg cgcactcttt ttaaaaccgc
     3961 gatcgcagcc ggagcagtcg gcgcaatcgg aggttatctc ggcggcaaaa aacagggcga
     4021 aaccgccgaa cgcaccgccg aaagccaaca ctcgccccaa gcctatccct gctacggcga
     4081 acatcaggca ggcatcgtta cgccgcagca ggcgttttcg attatgtgcg ccttcgacgt
     4141 aaccgcgcaa agtgccaagc agctggaaaa cctgttccgc acgctgaccg cccgcatcga
     4201 gtttctcacc caaggcggcg aataccaaga cggcgacgac aaacttccac ccgccggcag
     4261 cggcattttg ggcaaagcct tcaaccccga cgggttgacc gttaccgtgg gggtgggcag
     4321 cagcctgttt gacggccggt tcggactcaa agacaaaaaa ccggttcatt tgcaggaaat
     4381 gcgcgacttc tccaacgata agctgcaaaa aagctggtgc gacggcgatt tgagcctgca
     4441 aatctgcgcc ttcacccccg aaacctgcca agccgccctg cgcgacatca tcaaacacac
     4501 cgtccaaacc gccgttatcc gttggagtat cgacgggtgg cagcctaaat ccgaacccgg
     4561 cgcgatggcg gcgcgcaacc tgttgggctt cagggacggc acgggcaacc ccaaagtttc
     4621 cgaccccaaa actgccgacg aggttttgtg gacgggtgtg gccgccaaca gcctcgacga
     4681 accggagtgg gcgaaaaacg gcagctatca ggcagtccgc cttatccgcc actttgtcga
     4741 gttttgggac aggacgccgc ttcaagagca aaccgacatt ttcgggcggc gcaaatacag
     4801 cggtgcgccg atggacggca aaaaagaagc cgaccaaccg gattttgcca aagaccccga
     4861 ggggaatacc acgcccaaag acagccatat acgcctggcg aatccgcgcg atcccgaatt
     4921 cctcaaaaaa caccgcctct tccgccgcgc ctacagctat tcgcgcggac tcgcctcaag
     4981 cggacagctt gatgtcgggc tggtgttcgt ctgctatcag gcaaaccttg ccgacggatt
     5041 catcttcgtg caaaacctcc tcaacggcga accgctggaa gaatacatca gccccttcgg
     5101 cggcggctat ttcttcgtgc tgcccggcgt ggaaaaaggc ggcttcttgg gacaagggct
     5161 gctgggtgta taaacctgcc tgacggacaa atgccgtctg aatccgataa ggggttcaga
     5221 cggcatttta gtgtttgggc ggtcaggctt ttttgacgaa ttcagatttt aaattcatcg
     5281 cgctgccgtc gattttgcag gagatgttgt gatcgccttc ctgtaggcgt atgcctttga
     5341 cttttgtacc ttgtttgatc gtcatcgagc tgccttttac cttgaggtct ttgatgagga
     5401 tgacggtgtc gccgttttgc agcactgcgc cgttggcatc gcgcacttga gccgcaaggt
     5461 cggcggcgga ttcggtttca ttccattcgt gggcgcattc ggggcagatg tattgtccgc
     5521 cgtcttcata agtgtattcg gaggcgcatt gtgggcatgg gggtaatgac atggtttgca
     5581 gtccttatcg gatgtttgtt ttggggtacc gtctgaaacc tgaaaccggc ttcagacggc
     5641 atagctttat tgtttgtttt tttcaggcct cacccagccg tcgatgatgg tttggcggga
     5701 gcgggcgagg gcgagtttgc cgtcttcgac attgcgggta atcgtgctgc ccgcgccggt
     5761 ggttactttg ttgccgattt tgacggggga gaccaagacg cagtttgaac cgatgcgcac
     5821 ttcgtcgccg atgacggttt tgtgtttgtg cacgccgtcg tagttggcaa taatcgtacc
     5881 tgcgccgaag ttggttttgc tgccgacttc ggcatcgccg atgtaggtga ggtggttggc
     5941 tttggtgcct ttgccgatgg cggcgttttt gatttcgacg aagttgccga cgtggacatc
     6001 gtcagccagt ttggcttgcg gacgcaggcg ggcgtacggg ccgattcggt tgttttcgcc
     6061 gacttcgcag ctttcgaggt gggagaaggg ggcgattttg ctgtttgcac cgattttggc
     6121 gtttttgatg acgcagtttg cgccgatttc gacgttgtcg ccgagctcga tttcgccttc
     6181 aaagatacag ttcacatcaa ttacgacatc ctgcccgtgt ttcagacggc ctcgtaaatc
     6241 gaaacgcgcc gggtcgcgta gggtaacgcc tgctttgagc aattcttgcg cctgttcggt
     6301 ttggaagatg cgttcgagtt cggcgagttg gcgtttgttg ttcacgccgg cggcgaggta
     6361 ggaggtgcgc acttggacgg gatggacttt gatgccgtcg gcaacggctt tggcgatgag
     6421 gtcggtcaga tagtattcgc cttgggcatt gttgctggaa aggctgttca accatttttc
     6481 cagtttggcg ttgggcagga cgaggatgcc ggtgttggtt tctttcacgg ctttttgggc
     6541 ggtatcggcg tctttttctt cgacgatggc ggttacgctg ccgttgctgt cgcggatgat
     6601 gcgccccaag cctgtcgggt cggcgggcac gtcggtcaac agcccgactt tgttgcctgc
     6661 ggcttcgagc agggttttga gggtttcaac gtcaattaaa ggaacgtcgc cgtacaacac
     6721 cagcgtgcgg ccttcggcgg caaggtgggg cagggcggtt ttgacggcgt ggccggtgcc
     6781 gagttgttcg gtttgttcaa cccagacgac atcgcgtttg acggtgtcca aaacttgctc
     6841 tttgccgtgt ccgacgacga cgcagatgtt ttgcggattc agcgcggcgg cggtgtcgat
     6901 aacgcgcccg agcatgggct tgccgccgat gcggtgcagc actttgggca ttttggaata
     6961 catgcgcgtg cctttgccgg cggcgaggat gacgatgttt aaagtgtttt gcggcatagc
     7021 gttttcctgt gcaatgccgt ctgaaagggc ttcagacggc atagggtggg tttatcggtt
     7081 ttgaaatttg ggcttttgcc agtgttggcg atgctcttcg tcggcgttgt tgccggtttg
     7141 gttgggtaac acggcatggc gttcgggacg gtattggttg tagttcatat ttttcgagta
     7201 gctgccgtct tggtaataaa cgggcgtgcc ggcgggatat ttttgacgga cggcggtctt
     7261 gccgttgccg tcttgataag tttcccacgc gcagcccgac aaaagggcgg cggcagtcat
     7321 caggataagg aaggttttac gcataacatt ctttcgtatt ttcgggggtt gtgcgggtat
     7381 tgtaatgatt ttggcggtgt tctgacaaag tttctgcata ccgagccagt tgcgccatat
     7441 cgcttacgga agcatcgata aagggcaggt cgtgcacttt tgcaccgaac cggacggttt
     7501 tcatgcccaa tgcttttgcc tgatgcaggt tgtccgcgct gtcgtccacc ataatgcagc
     7561 attcgggcgg tacgtccaaa aggcggcaga cattgagata cgcttgcgga ttgggtttgt
     7621 acagcagccc gaaatcatcc gtgccgaaca gtgcgtcgaa acggttttcc aaaccgagtg
     7681 cgccgacaac ggcacggacg taaaacgacg ggccgttgga aaaaaccgcc ttgcgcccgt
     7741 tcagacggca tagggtgttt tccgtatccg ccatgccgtg cagcctggtc aggattgcat
     7801 cgatcggatg gctttcgcgc aaaaattcgg cgatgtcgat ttcgggatgg tggatttgca
     7861 gtccggcgag cgttgcgccg tagcggtgcc aatagtcttg acgcaggtcg gacgcggcag
     7921 attcggagag tttgaggcgg cgtgccatat agcgtgtcat agcgcggttg atgagtgtga
     7981 agaggccggc atcggcattg tgcagcgtgt tgtcgaggtc gaacagccac acggtcgggt
     8041 tttcttgcat gttgaaccgt gaaaatttgt tagaatgtta ttttacagcg aatagaggag
     8101 gactcggaat gaaacggaaa atttggctgc tgccgctgct ggcggtttcg gcatacctgc
     8161 aggcgcagac ggaagtcagg ctggcggtgc ataagtcgtt cagcctgccc aaagggttga
     8221 ttgcgcgctt cgagcgggca aacgatgcga aagtgtcgat tattcaggcg ggcggcgcga
     8281 acgaaatgct caacaaactg attttgagcc gcgccaaccc gattgccgac gcggtgtatg
     8341 gtttggacaa cgccaatatc ggcaaggcgc gggaaatggg cattttggcg gcggcgcaac
     8401 ccgaatccgc ccccgtcgcg gtcgggctgc cttcggcttt ggcggtcgat tacggctatg
     8461 tgtccatcaa ttacgacaaa aaatggtttg aaggcaaaaa gctgcccctg ccgcaaaccc
     8521 tgcaggattt gacccgcccc gaatataaaa acctattggt cgtgccgtcc cccgccacgt
     8581 cgtccccggg gctgggcttc ctgatggcga acatcagcgg tctgggcgaa gaaggcgcgt
     8641 tcaaatggtg ggcacagatg cggcagaacg gcgtgaaggt cgccaaaggt tggagcgagg
     8701 cgtattacac cgacttttcg cacaacggcg gcgcgtatcc gctggtggtc ggttatgccg
     8761 ccagcccggc ggcggaagtg tatttttcca aaggcaaata cagcgagccg ccgacgggca
     8821 acctgttttt aaaaggcggc gtattccgcc aggtcgaagg cgcggcggtc ttgaagggcg
     8881 cgaaacagcc ggaattggcg gcaaaactgg tgcaatggct gcaaagtcgg gaagtgcagc
     8941 aggcggttcc gtccgaaatg tgggtttacc ccgccgtcaa aaacacgcgc ctgcccgacg
     9001 tgttccgctt cgcccaagcc ccgacgcaca ccaccgcccc cgcgcagcgc gatattgatg
     9061 cgaaccagcg cggatgggtt tcccgttgga ttagaacggt tttgaaataa aacaaacata
     9121 ccgcctcgca gggcttcata cggcattttt acacctgtgc cgattacgcc gcacggggcg
     9181 gatgttcgat caagaggaaa acaatggact tcaaacaatt tgatttttta cacctgatca
     9241 gtgtttccgg ttgggagcat ctggctgaaa aggcgtgggc gttcgggctg aaccttgccg
     9301 ccgcgctgct tatttttttg gtcggaaaat gggcggcgaa acgcattgtc gctgtgatga
     9361 gggcggcgat gacgcgcgcg caggtcgatg ccacgctgat tagttttttg tgtaatgttg
     9421 ccaatatcgg cttattgatt ttggtgatta ttgccgcatt gggcagattg ggcgtttcca
     9481 caacatccgt aaccgcctta atcggcggcg cgggtttggc ggtggcgttg tccttgaaag
     9541 accagctgtc caattttgcc gccggtgcgc tgattatcct gttccgcccg ttcaaagtcg
     9601 gcgattttat ccgcgtcggc ggttttgaag gatatgtccg agagattaaa atggtgcaga
     9661 cttctttgcg gacgaccgac aacgaagaag tcgtgctgcc caacagcgtg gtgatgggca
     9721 acagcatcgt caaccgttcc accctgccgc tttgccgcgc ccaagtgata gtcggcgtcg
     9781 attacaactg cgatttgaaa gtggcgaaag aggcggtgtt gaaagccgcc gtcgaacacc
     9841 ccttgagcgt tcaaaacgaa gagcggcagg ccgccgccta catcaccgcc ttgggcgaca
     9901 atgccatcga aatcacatta tgggcttggg caaacgaagc agaccgctgg acgctgcaat
     9961 gcgatttgaa cgaacaagtg gtcgaaaacc tccgcaaagt caatatcaac atcccgttcc
    10021 cgcaacgcga catacacatc atcaattctt aaacgccgtc tgaaagggga gtgggaaaat
    10081 ggacgcgctg cacaccatcg cccgaaacct gacgaaaaaa cgtcaaaccg taagctgtgc
    10141 cgagtcctgt acgggcggaa tgcttgccgc cgcattcaca agcgttgcag gcagttcgca
    10201 atggttcgac cagagttttg taacatacag caacaaagcc aaagaagacc gcttgggcgt
    10261 gttgcccgaa accctgctcg aacacggcgc ggtcagccgc caaaccgtct atgagatggc
    10321 gcgcggcgcg aaagccgtgg cgcaggcgga ttacgccgtc ggtatttccg gcatcgccgg
    10381 accgggcggc ggcagcgaaa gcaaacccgt cggcacggtt tggttcgggt ttgcctttcc
    10441 gggcggaagt tgcgaagcaa tgcgccgttt tgacggcaac cgcgaatccg tccgcgcgca
    10501 ggcggtcgcc ttcgcgttgg aacggttggc ggggctgatt gaaaacggcg gcgatgctgt
    10561 ctaaacaaaa tctccgtctg gacagaatcc ccgccggata aaaaataccg tctgaaacgt
    10621 tgcaggtttc agacggtatt ttgtcgggtt aggcggcggt gcggcttatt tcactttact
    10681 cttcaacgcg ccgtagcctg ccgcgtccat ttgttccagc gggatgaatt tcaagctcgc
    10741 gccgttgatg cagtagcgca gtccgccttt gtcgcgcggg ccgtcgggga agacgtgtcc
    10801 caaatgcgaa tcggcggcgt ggctgcgcac ttcggtgcgg cgcatgttgt agctgaaatc
    10861 atcgtgttcg gtaacggatt ttgcatcaat cgggcgcgtg aagctcggcc agccgcagcc
    10921 ggaatcatat ttgtcggcgg agctgaacaa aggttcgccg ctgacaacgt ccacataaat
    10981 gccgggtttg aacaaatggt cgtattcgtg gctgaaggca tattcggtcg cgctgttttg
    11041 ggtaacttgg tattgctctt cggtcagggt gcgtttgagt tcggcgtcac tcggtttttt
    11101 atacgttgcc gcatcgaagc ctttgccttg cggggcggtc ttggttttgc ccggcagcgg
    11161 ttcgtcggct ttgcggatat cgatgtggca gtagccgttg gggtttttaa tcaggtagtc
    11221 ctgatggtat tcctcggcat cgtagaagtt tttcagcggc tcgttttcaa caacgagggg
    11281 cagttggtat ttttgctgct cgcgtttgag ggcggcggcg atgacggctt tttcggcggg
    11341 gtcggtgtag tacacgccgc tgcggtattg cgtaccggtg tcgttgccct gtttgttgag
    11401 gctggtcgga tcaacgacgc ggaagaaata ttgcaggatg tcgtctaggc tgagtttgtc
    11461 ggcatcgtag gtcactttga cggtttcggc gtggcccgta tggcggtagg acacgtcttc
    11521 atagctcgga tttttcgtgt tgccgttggc gtagccggat accgcgtcaa ccacgccgtc
    11581 gatgcgttgg aaataggctt ccaagcccca gaagcagccg ccggcgaggt aaatggtgcg
    11641 cgtgttcatg atttttgaat cctttttctg agtgtcgggt ttgtagaacg aatgtttcaa
    11701 gctgcccaaa tcggcattcg ggtcgcggat taacgccaac gcctgcgctt cgttgatgct
    11761 gcctttgacg atgcgctgca cgtcgccgtc tttaccgatt aacgcccacg aagggtaaac
    11821 gctgatattc aggctttggg cgatcgtgcc gccgttgtcg gttacgacgg gcagcttggg
    11881 ataattcaaa ccggcatacc atttttggaa gtcgccgtct tttttctcgt gcaaaaagcc
    11941 cggagacgcg acggtaatca ggttggcgga gctgaatttt gcatcttgcg cccatttttc
    12001 ggtctgtccc aattcggaca aacacaaagg acaccagctc gcccaaaatt taatcagcgt
    12061 cggtttgtct tttttcaagt aaacactggc ggggcggttg tccgcagttt tcaaagtgga
    12121 taaagtgtgc ggcacggtcg cggctccggc atcgacgatt ttgggcgaac aagcacccag
    12181 cgcaagcagg cagccgaact tggcgcaaag ggaaaagaaa gtacggtgtt tcattttgag
    12241 tttcctgtgt ggacggtttg catgattaga cgtttgagat gccgaaacct tacagctcgg
    12301 attttcagac aaccttgccg cgtaaaatac gctacaatac gccctgtttc aagtttctaa
    12361 aattaaaagg aaaattcaat gttcagcttc ttccgtcgca agaaaaaaca ggaaacgccg
    12421 gctctcgagg aggctcaaat tcaggaaacc gcagcaaaag cagagtccga aaccgatcaa
    12481 gtaattgaaa atattaaaga agatgctgaa tctttagcag aaagcgtcaa agggcaggtc
    12541 gaatctgccg ttgaaaccgt cagcggtgcg gttgaacagg taaaggaaac cgttgccgag
    12601 atgctgtctg aagcagagga agcggcggaa aaagcagcgg aacaagtcga agcggcaaaa
    12661 gaagccattg ccgaaaccgt cggcgaggct gtcgggcaag ttcaagaagc cgttgcgaca
    12721 actgaagaac acaagctcgg ttgggcggcg cgtttgaaac aaggcctgac caaatcgcgc
    12781 gacaaaatgg cgaaatcgct ggcgggcgtg ttcggcggcg gacaaatcga cgaagatttg
    12841 tacgaagagc tggaaaccgt gctaattacc agcgacatgg gcatggaagc caccgaatac
    12901 ctgatgaaag acgtgcgcga ccgcgtcagc ctcaaagggc tgaaagacgg caacgaattg
    12961 cgcggcgcgt tgaaagaagc cttgtacgac ctgattaagc ctctggaaaa accattggtc
    13021 ttgcccgaaa ccaaagagcc gtttgtcatc atgcttgccg gcatcaacgg cgcgggcaaa
    13081 accacgtcta tcggcaaact cgccaaatat ttccaagcgc agggcaaatc cgtattgctg
    13141 gcggcaggcg acactttccg cgccgccgcc cgtgagcagc ttcaggcatg gggcgaacgc
    13201 aacaacgtta ccgtgatttc gcaaaccacg ggcgattccg ccgccgtgtg cttcgatgcc
    13261 gtccaagccg ccaaagcgcg cggcatcgac attgtgctgg ccgacaccgc cggccgcctg
    13321 cccacgcagc ttcatttgat ggaagaaatc aaaaaagtga aacgcgtgct gcaaaaagcc
    13381 atgcccgacg cgccgcacga aatcatcgtc gtgcttgatg ccaacatcgg gcaaaacgcc
    13441 gtcaaccaag tcaaagcctt tgacgacgca ttagggctga ccggtttaat cgtgaccaaa
    13501 ctcgacggca cggcaaaagg cggcatcctc gccgcgcttg cctccgaccg ccccgtcccc
    13561 gtccgctata tcggcgtggg cgaaggcata gacgatttgc gcccgtttga cgcgcgcgcg
    13621 tttgtggacg cactgctgga ttgagccgaa atgccgtccg aaaacagcag accgatgccg
    13681 tcattcccgc gcaggcggaa atccagacct tgggataacg gcaatattca aagtttataa
    13741 aagacccgtc attcccgcgt aggcgggaat ccagaccttg ggataacagc aatattcaaa
    13801 gtttataaaa gacctgtcat tcccgcgcag gcgggaatct agaaccatcg gcacggaaac
    13861 ttatcgggta aacggtttct tgagattccg cgtcctagat tcccactttc gtgggaatga
    13921 cgg
//
LOCUS       DAWKQJ010000040        13815 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000040 DAWKQJ010000000
VERSION     DAWKQJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.040"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="WJL20_000885"
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="WJL20_000885"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ7683055.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1611..2486)
                     /locus_tag="WJL20_000886"
     CDS             complement(1611..2486)
                     /locus_tag="WJL20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7683056.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2488..3210)
                     /locus_tag="WJL20_000887"
     CDS             complement(2488..3210)
                     /locus_tag="WJL20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ7683057.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQQAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNDGRAVAPEKWI"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WJL20_000888"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WJL20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ7683058.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFSRLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3787..4149
                     /locus_tag="WJL20_000889"
     CDS             3787..4149
                     /locus_tag="WJL20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7683059.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKRDAATA"
     gene            4184..5047
                     /locus_tag="WJL20_000890"
     CDS             4184..5047
                     /locus_tag="WJL20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ7683060.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLAADGEEAGIVLDSVKDSESFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5145..6104
                     /gene="ttcA"
                     /locus_tag="WJL20_000891"
     CDS             5145..6104
                     /gene="ttcA"
                     /locus_tag="WJL20_000891"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ7683061.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6238..6792)
                     /locus_tag="WJL20_000892"
     CDS             complement(6238..6792)
                     /locus_tag="WJL20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7683062.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNIPVQPVKIRRVVAE"
     gene            complement(6857..7774)
                     /locus_tag="WJL20_000893"
     CDS             complement(6857..7774)
                     /locus_tag="WJL20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ7683063.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(7957..8229)
                     /locus_tag="WJL20_000894"
     CDS             complement(7957..8229)
                     /locus_tag="WJL20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ7683064.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            complement(8226..8519)
                     /locus_tag="WJL20_000895"
     CDS             complement(8226..8519)
                     /locus_tag="WJL20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ7683065.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            complement(8564..9040)
                     /locus_tag="WJL20_000896"
     CDS             complement(8564..9040)
                     /locus_tag="WJL20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ7683066.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            complement(9111..9566)
                     /locus_tag="WJL20_000897"
     CDS             complement(9111..9566)
                     /locus_tag="WJL20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ7683067.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(9691..10806)
                     /locus_tag="WJL20_000898"
     CDS             complement(9691..10806)
                     /locus_tag="WJL20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ7683068.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(10920..12017)
                     /locus_tag="WJL20_000899"
     CDS             complement(10920..12017)
                     /locus_tag="WJL20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ7683069.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(12099..13682)
                     /locus_tag="WJL20_000900"
     CDS             complement(12099..13682)
                     /locus_tag="WJL20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ7683070.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDADLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
BASE COUNT         3382 a         3773 c         3306 g         3354 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacactctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc cgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaagcaaccg cgcaggcaaa cggcgaattg ggtgcagtaa aacccgaaat cgccgccgcc
      361 attgaaaagg cttgcgacga agttctgctg aacaaccgct gcctcgacca attcccctcc
      421 gacgtgtatc agggcggtgc gggaacttcg gttaatatga acaccaacga agtcatcgcc
      481 aacctcgcat tggaagcctt gggatacgaa aaaggccgct acgacatcgt caatccgatg
      541 gatcacgtca acgccagcca atccaccaac gatgcctacc ctaccggctt ccgccttgcc
      601 gtgtattaca gcatcggcga attgctcgac aaactgactg tcctgaaaaa cgcctttgcc
      661 gccaaagccg aagaatttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtgccgatga ctgccggcca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacccg ccaactgctg ttggaagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattgcagg ccggttcttc cataatgccc gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgacacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacc
     1261 atttccctct tgggcaatgc cgcagtcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatctgcga acgttacgtt ttcaactcca tcggtttggt gacttatctg
     1381 aatccgtata tcggacacca caacggcgat ttggtcggca aaatctgcgc ccaaaccggc
     1441 aaaggcgtgc gcgaggttgt cttggaacgc gggctgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ccgagaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcaggga tgctgcttca gacggcattt tgttatatca atcagccgga ttatatgccg
     1621 aacagtgcct tgacctccgc ccaataaaac aggcaggcga tggaacacag tgccaacacg
     1681 attcccgccg tattgattgt gttgattttt tccttaaaaa acagtgcgcc cgaaagcgtc
     1741 cccaaaacaa tcacgccgat attcataccg gcaaaaacca gcgtgggatt gtccttcatc
     1801 atttggtgcg cggtgatgta ggttacgatg ttcatgaagt tcaaaccgcc caagagtatg
     1861 ccgccgacca cactctcaac gcgccatctg accgatttgg caaacaggca ggcaaacatc
     1921 agcacacccg ccagcacaaa tgcaaccagc aggttgcccg caaatgccgt tccgcttttg
     1981 gcaagctgtt tgaacaggat atcgataatg ccgtaacctg cccacacgcc cagcagcaat
     2041 gccgcctgcc gccacgcgct tcctgatttt ttgccaccgc tgtgtttcca aagcaggcag
     2101 aacagtgcgg caaatgcgag gcacagcccg attagtctgc cttcgctgag tttttcgcca
     2161 aacagcgtca aggcggcaac aatcggcaaa aacagcgaca aacgctgcgc cgcgtcggat
     2221 ttgacgatac cggcggattc tacggatttg cccataatga caaatacgga cggcagcagc
     2281 acgcctaatg cggcaaacag cggccacgtc ggcaaaaatg cgccgatatt gccgacatcc
     2341 ggcttcaata ccagcaaagt cagtatgacc gcgaccacat aattgacggc gaccgcctgc
     2401 gcgatgtcga tttttttctt ccttgccatt ttcagcaaaa tggaaaccga cacgctgaac
     2461 acgatgctga ttaacaggta agccatacta tatccatttc tcgggtgcaa ccgccctgcc
     2521 gtcgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgt tcgttgcaat
     2581 aataatcagc acgccgatac ccaaaagggc agtcagcgcg cccaaaagat aaagccaaaa
     2641 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
     2701 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatatagg cgaaaatcac
     2761 gccgactatg ggcatgaaca gcatgattgc acccaaaccg aacaggatgt aggcggcgag
     2821 gacatagttg cgcccgcttc cgccgccgga cggttcgtaa gcatggcgcg aacgtgaggc
     2881 ctgccgttca aacgcctgtc tgaacgcctg ttcgcggcgc atctgttcgc ggtaaaactg
     2941 ctccctgcgc gcctgttcgc ggcggaatgc ctcttcctgc cgcccgcgtt cctcatgccg
     3001 tctgaaggac gcgtcgtatt gcatccgttt cgacaggtcg gaaagcgtat cgtaagcctg
     3061 ttggatttct ttgaactttt cttccgcctg cggattgccc ggattgcggt cgggatggta
     3121 tttcatcgcc agcttgcggt aggcgcgttt gatttcgtcc gctcccgcct gcggcgacac
     3181 gcccaatacg gcatacaaat ctttgtccat attgttcctt tatactttcc cgtcaaaccg
     3241 ccgcctgtat tgttttcggg ttcaaaatat ttaaatctcc gggacggatg ccgataatga
     3301 acccgcgttt tccgccgttg atatagatgg tttccaaatc catcaccgac tgttcgacgt
     3361 aaatatccaa ctttgtccgg atgccgaacg gcgttgtgcc tccgaccaga tagcccgtcc
     3421 acttgtttgc ctgtgcgggc gtagcgggtt cgatgtgttt cgcacccaaa tgccgagcca
     3481 gattgcgcgt ggaaatctgt ttgtcgccgt gcatcaagac aatcagcccc ttaccgtttt
     3541 catcttgcaa aacaatggtt ttaatgacca agtgttcgtc tttgccgaac agtcgggaaa
     3601 actgcgccgt gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt
     3661 tttcacgcaa aacgcgcacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc
     3721 atgcaaaaca tggaaatcgg ccaagcgcaa tgttaccata tatccccttt atcgaaggaa
     3781 accgatatgg atatccgata tttcggcaca acgccccgct attccgaagc cgtcggcgca
     3841 aacggcttga ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag
     3901 actgccgacg tgcttgccca aatcgaccgc tggctggcgg aatgcggctc ggacaaagcg
     3961 cacgttttgg atgcggtcat ctatctgcgc gatatgggcg actatgcgga aatgaacggc
     4021 gtgtgggacg cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagctcgg
     4081 ctcgcccgtc cggagtggtg cgtcgaaatc aaaataaccg ccgtgaagag agacgcggca
     4141 actgcataaa cagatttcct ttatatttca gagtacgcaa atgatgaaaa ccttactgcc
     4201 ctttttcggc gtggtgcgcg tcagcggcga agaccgccaa accttcctgc acggacagct
     4261 ttccaacgac atcaaccacc ttcagaccgg gcaggcgtgt tatgcgactt acaacacgcc
     4321 caaaggccgc gtcatcgcca atatgattgt cgtcaaccga ggcggcgatt tgctgctgat
     4381 tatggcgcaa gacctgcttg aagcaacggt caaacgtttg cggatgtttg tgttacgcgc
     4441 caaagccgtt tttgaaatcc ttgaagatta cgccgtcggc gcggaattag aagcatccgc
     4501 cgaaccgctt gccgcgcaag aacccagcct cgcctttacc gccgaatgcg gttcagacgg
     4561 catctgttcc gtcgtcctgc cacatcgggg cattttgcac atcgccccca aaaacgcctt
     4621 gccgccttat gatgccgccg ccgaaaacgc gtggcggctg cacgaaatcc gcagcggtta
     4681 cccgtggata tgcgcggcta ccaaagaaac tgccgttgcc caaatgctca accagcacat
     4741 catcggcggc gtacacttca aaaaaggctg ctatcccggt caggaaatca ttgcccgtgc
     4801 ccagtatcgc ggtcaggtca aacgcggctt ggcggtattg tcgggaaatt cggcagccga
     4861 agcaggcatc ctgctggcgg cagacggcga agaggccggc atcgtgctcg acagcgttaa
     4921 agattcggaa agctttaccg cccttgccgt gattaaattc tccgccgcac aaaaagaact
     4981 gaccgcccca aacggcagta tcttcaaagc ggttcatccc ttctttaaaa ccgaaaatgc
     5041 agaataatcc ctctaatgcc gtctgaacct ttttcagacg gcattttaaa accgcccctg
     5101 ttttcagtta caatgccgca tccccaattt ttcaaaccac cgccatgtcc aaaaaaacca
     5161 aacaagaact ggaaaacaac aaactcagca aacgcctgcg ccacgccgtc ggcgacgcga
     5221 ttaacgattt caacatgatc gagccgggtg acaaaatcat ggtctgcctc tccggcggca
     5281 aagacagcta cgccctgtta gacatcctgc gccggcttca agccagcgcg ccgattgatt
     5341 tcgaactggt cgccgtcaat ctcgaccaaa agcagccggg cttccccgaa gaagtattgc
     5401 cgacctatct cgaaagcatc ggcgttccct acaaaatcgt tgaagaagac acctactcca
     5461 ccgtcaaacg cgtattagac gaaggcaaaa cgacttgttc gctgtgcagc cgcctgcgcc
     5521 gcggcatcct ctaccgcact gcaaaagaat tgggctgcac caaaatcgcc ttgggacacc
     5581 accgcgacga catcctcgcc accttgtttt taaatatgtt ttacggcggc aaactcaaag
     5641 ccatgccgcc caagctggtg agcgacaacg gcgaacacat cgtcatccgt ccgctggcgt
     5701 atgtaaaaga aaaagactta atcaaatacg ccgaattaaa acaattcccc atcatcccgt
     5761 gcaacctctg cggctcgcaa cccaacctgc aacgccaagt catcggcgat atgctgcgcg
     5821 attgggacaa acgcttcccc ggccgcatcg aatcgatgtt ctccgccctg caaaacgtcg
     5881 tcccttccca ccttgccgat accgaactct tcgactttgt cggattggaa cgcggtcaaa
     5941 ccctgaaaca cggcggcgat ttggcgtttg acagcgaaaa aatgcccgaa cgtttttcag
     6001 acggcagcga agaagacgaa agcgaaatca aaatcgagcc gcaaaaagcc gaacgcaaag
     6061 tcatcaacat tctggcgaac aagccgaaaa cctgcggcgc gtaaacctcc accgtcaaaa
     6121 atgccgtctg aagcccgttt ggcgtttcag acggcatttt aaaatcccga agtagacact
     6181 tttcaacccc aaacgcctgc tttttccgtt tcagctttct gaaggtttcc cgttatatta
     6241 ctcggcaaca acgcggcgaa ttttgacggg ctgtacgggt atgttttgat aaaagccgcg
     6301 cgtggcggtt ttgacgcggg cgattttgga aacggtgttc atgccgcttt cgaccctgcc
     6361 gaaaacggta tagccgtatt gtccgttttt gtagtcgagc gaagcgttgt ccgccagatt
     6421 gataaagaat tggctggtgg cggaatcggg ggctgtcgtc cgcgccatgg cgatggtgcc
     6481 ggcggtgttt ttcaagccgt tgccggattc gttggcaacg gccttatcgc ttgccttttg
     6541 tgccaagtcc tcggtcaatc caccgccctg gataacaaaa ccgtcgataa cgcggtgaaa
     6601 aacggtgtcg tcgtaaaagc cttttcgggc atagcgcacg aaattagcaa cggttttggg
     6661 ggctttggat tcgtccaaaa ccaaacggat attgcccata tcggtttcca tcaaaacatg
     6721 ggttgccgcc atagacggca gggaaaccgc caaaagcagc gcggttaaaa cggttttgaa
     6781 tttgggtttc atcccgtcct cctcagacct tcagacggca ttttcatttc ctatgccgtc
     6841 tgaaggctcg ttaacgctat tccaatgcgt ctttgagttt ttgttcgatt aaatccgcat
     6901 caaacgattt ggcaatcaat tcaaaacgcg agtcgcgccg ccaagacact tggttcgcac
     6961 cccattgccc gtccacccag ttgagccaca cccacgttcc caatacttgg aacacgcctt
     7021 tggcacggac gagtccttcg gtcatattgg gcaaatcatt gaagaagttg gtcaattttt
     7081 caccgtcgaa atcgcgtccg gcggggaatg tgaaaccttg cgactggaag cccatcgtgt
     7141 tgtccggcag ggctttgagg cggtagcgtg atttttcgat gacggggatg tcaagccatt
     7201 ggatatcgag ttgtgcgttt tgaacttcga ccactttagc cttgggcggg aacagttttg
     7261 cggctttgtc gtgaaattcg gcaagctgtt cgggggtgca taaatcggtt ttgctggcaa
     7321 ccaatacgtc gcagatgccg atttggtctt tatacaatgc ctgctgcgcg taatcggggt
     7381 tgatgaactg gcgcggatcg acgacggtaa agactgcgcc gatttccaaa aggctgtcca
     7441 gcggtttggt tttcagttca tcaatgacac tggcggcgtg cgccagtccg cttgcttcaa
     7501 tcatcaggcg gtcgggcttg gcgtcgcgca gcattttctg cacggttacg cccatttgcg
     7561 ggccggcggt gcaacacaaa cagccgccgg cgatttctgc cacagggatg ccgttgtcgc
     7621 tcaataccgc gccgtcaatg ccgatttcgc cgaactcgtt gacgatgatg acccattttt
     7681 cgttcgggtc tttttgcgcc atcaggcttt tgagcgcggt ggttttgcct gttcccagaa
     7741 aacctgaaat caggtggact ttggtttttt tcatttctat gtgatgtccc actttaaaat
     7801 ttgaagatag ggtgttttaa atgattaaat aatgttaaaa gtgatggcag ctgttatcat
     7861 gttcttcatc aattgacaat tgttccagca aattcgatat atcggctgac atcgccggta
     7921 tgacgttcaa aaccgtctcc caaacgacag acaggttcat ttcaaagtag ccatgggcaa
     7981 tcctattgcg caataccccc tcatccctat ccaattcaaa tatttggttt cctcggcaaa
     8041 ttccggatac gatttaagga tatgggttgc gttttcaccg ataaggaaca aattaaaaac
     8101 gactgcctgc acagtctttg tgtcggcaga aaattgccca taatccattt tgtcggtata
     8161 tagccggata tattgcgctg cctgcaatat ctgtttcaaa tagaccgaca attctttctg
     8221 cattttcata atgcgacagc ctcagtcaac accttatctc taaagtgggc cgagatgtca
     8281 tcgggtgtca gcaaatcgac ctttatgccc aagagtttct gcaattcctc ttgcagcccg
     8341 cccaaatcca ataatgttgt ccctgttttt gcatccacca acaaatcaat gtcgctgttt
     8401 tccgtatcat ctccgcgaga aaccgaaccg aatacccttg gattgcaaat caatggatat
     8461 ttcccgaaaa ctgccaatat ttctttcttt ctgctttgca acaaaagaga cggtttcatt
     8521 atctgctcct ttcgaaaggc ttattatcaa tgcaaaccac cgattactgc tgacattttt
     8581 tacaaatccc ggttaaaaca acgtgttctt ctttcagcgc aaagccgctt tcggcaacgc
     8641 ctgcgcgcag tgccgcccac tcgtggctca gggtttgctc gtccgccgtg ccacattcgg
     8701 tgcagaccaa aataaacgcg ctgtggtgcg cttcggcttc ttcgtggtcg tggcaatggt
     8761 cgtcgcactc gtgctgcgcg tggctgcaca aaatatagcc gttgaccgcc gccactttgt
     8821 gcaaaacgcc ctgctccgcc caaaaatcaa gggcgcggta ggcggtaggc ggtgcaagca
     8881 cgccctcgct ttgctgctgc atctgcgaca agacgttgta ggctttaatc acgccgcttt
     8941 gctgcaagac aatatctaat acctgctcgc gcaaagcggt tacctgcaag ccttcgctgc
     9001 gtgcctgttc gataattttc tgtttgaaat ttgttttcat aaattctctg tttatgccgt
     9061 ctgaacaacc gatacggcag gaggcggttt tatatttgta ttcaattgct ttatttggaa
     9121 atcttttcca acaatgcccg acagcccgca tccgcaagcc tgaaggtttc ttcaaaatcg
     9181 cccgtatacc acggatcggg gacatggtcg taaccgcttt cgggtatcag gtcggtcagc
     9241 ttgaatattt tttccggccg cctgccgaag gttctttcca attcggacaa attcttgccg
     9301 tccatcgcga tgatgcagtc aaacgccgcc gcatcgcttt ggcggatttt gcggctggta
     9361 aagcctgaag catcgatacc gtgttttttc aatatctttg ccgtctcgcg gtgcatatct
     9421 tcgccgtcgt gccagcccga tgtccccgcg ctgtccgctt caaggggaat gcccgcttcg
     9481 gcggcgcggc ggcgcaaaat gtattccgcc atcggcgaac ggcagatgtt gccgaggcag
     9541 acaaaaagga ttttcggttt tttcatatcc cctccctgtt ccggcgcgat gccgtctgaa
     9601 gcggaaaccc tttcagacgt catcggtcgg ttaatcgtaa ccgcataagg cagaaccagc
     9661 caaaacatcc atctggaaga aatggtttgg ctaaatctta ggcatattta ataagtgtcc
     9721 aatattagaa gccgtatgct ccaaatagag actggcattt ttcaaactat cttctaaagg
     9781 ttcacttttc tccaaaatag aaaaggcagc ttggatattt tcaaatggca gggaaggcaa
     9841 atcttcaacg agactgccac aaatagcgac aacaggaact ccgacagggg ttctttttgc
     9901 tacaccaata ggcgctttcc ctgctaaact ttgacgatct agtcttcctt caccaacgat
     9961 aaccaagtca acatctgaca ctttcttatc aaagtcaatc aagtccaggc aggtgtcaat
    10021 tccagatacg atacttgcct gagcaaaggc gcacaaacca ccagcgatgc ctccaccagc
    10081 tcctgctcct ttaagtttta atgttgcagg ggagactttt tcataaaaat cttgtattgc
    10141 ctgatctacg gcctcaaaca tagtagaatc caaccctttt tgcttgccaa acgtataggt
    10201 cgcaccttgg tgtccacata agggactcac aacatctgct aaaatacgaa tgtgaacatc
    10261 ttcaggaatt ttatagcgat tttctgttga aacagaagct aggtttagta aggattgacc
    10321 gcaagcgggt aaggcatttc catcctcatc ataaaattga taacctaaac cagcagcaat
    10381 cccaatacct ccatcattac tggccgtacc gccaacgcca atatagattt ctttaatttc
    10441 ttgactaatg aggtggcgaa tcaattctcc aataccacga gtttggattt gtaatggatt
    10501 tcgtttctct agcgggattt ttccaagacc aaccaaatca gcaacttcga atagggctag
    10561 ttgttctttt tgaaaatagc gcatgacttc tttttgtcca aaaggtcctg tcacttggag
    10621 acatttttct tctaggtcaa gagaatgtcg gatagcatct acagtgcctt ctcccccatc
    10681 accgacagga cagaggagac attccacatc tgctatcgat tgttggaagc ctctttttat
    10741 tgcttcagct acctgttgag ctgtcaagct ttccttaaac gaatccggtg caattacaat
    10801 cttcatattt ataattcatc ctttcgtttc actcaaggca caacacagaa tgaaaaagtg
    10861 ttgtgctctt tattttgatt tattatagaa atgagaaagc ctatcactac tacaaatcac
    10921 tatgcgctga aaaacggatt gtgcccttcc cgtttcaatg cttccgcata gctcgggatg
    10981 ctttcctgtt cgcccaaggg attgtgcaac aggtaaacgt gttctacacg gcattctgcc
    11041 attaaatcca aaaaattttg ctgaataacg ccaatattgt cggtaaagga aagctgccac
    11101 gaaaacacct gcggcacgac ttcaaacgcg ccgtcggtcg cattaaaccc gaataaaatc
    11161 tgtccgcccg cctttgccgc tgcgaccacg ccgacgcgcg gactctgcat acggacggcg
    11221 cgtatcgcgt ttgccgcaaa cacatccatc gccatgcgct ggcgcgtgtg gctgccgtcg
    11281 gaaagcgcgc gtaccggaga atccggggaa agcggattgg caaacagcgt aacgccttcc
    11341 gatgcaagat tttcctgcca tacctgcatc aagggcagac ctgcctgaag aaggttctga
    11401 cccaatgccg tctgaacttt gccgctgccg aaacccagcg cgtaaacgac gtggacggac
    11461 tgcccttcgg gcaaaagcaa agggcgcggg gcgaaacggc gtaagtgttg cgccgccgct
    11521 tcggtatttt gtttggcacg ccaccactca tccggcgcga ccgcactcaa atcggaggaa
    11581 tgtacaagat aaggcagcca ttgcgtctct tgcgtcaacg cgcgcaggtg cggatagttt
    11641 tgcaaacagg caaacaatcc ctccgtcggc agcttcatct cgattgcccg ttctttcttg
    11701 cagccggaca ccagcacgac cggcagggcg aaccactgca cttcgccttc tttctcgcaa
    11761 tcgagtaccg cgttcacact ggaaagcagc gcggcataag ttccggcatc gggcgacatc
    11821 gtcagcgcga gggaaaggtt gatatagtgg ttttgctcaa gcatccccct gatttcggtt
    11881 tgaagttggc cggacgagag tttgcgcgaa gcctgggaag aattatgcgc caactggtag
    11941 gcattgagca gcaggtggtt tttaatcgga ttttggggat acgggcgcgt atcgggcaag
    12001 gtaaatgtct ggttcatata tgtcgtgtac cgcttttcgc agtgtgtgtt tttcctgttt
    12061 gaaaatatat cggacggatt gccgtcggga cttgcccgtc aatccgccga aacgagaaaa
    12121 tgcctgtctg ccaagtctgc caatatttct tccacataca cttcggcagg cggatggaat
    12181 gtcaaaccgt cgggcgtggt gctgacgatg ttcaagtttt cggcaaccgt ttcaatcaat
    12241 gcctgtttgc tcgtcggccg cgacaattgc cgcataatgt acaaatgccc gtacccgaat
    12301 gagggggtgc tttcgttgta acggttggca ggctggacaa acgcttccgc gccgtgttcc
    12361 acaaaggcgg cgacataatc tgtaaaacgc gccggcacat aggttttatg gtcttcaaaa
    12421 aattgtttgc ccgcattgtc tgatgaaaca gaaaaacgcc cgagaatggt ttgcaataaa
    12481 agctgggaat tgattttcat acggtcgaat gtcaaaccgg gaaatcggcg ggcgagattt
    12541 tccgcaacat cttccgtttt aaacggctct ccggttttca tcacatcgcg tatgcccgaa
    12601 agcagggtat cgttttcatc aaagttgatt gtttcccctc ttgccgggcg gaaattcaaa
    12661 ctttctatca cttcgacggc aaccgactcg tcacgcctga cagtatcccc gacttcctca
    12721 cggcataaaa gcgaacggcg gaattggcgg tcggataaaa tatcgctgta aaattctttg
    12781 gcaatataat cgccccctgc caatgccaga atccgctccc gcgtatgctc cgccatccaa
    12841 gaaacaaaag acgcgtgcaa atcggcatcc ccgatatacg ccagccggtg gcggtttgcc
    12901 cattcgatga agccgttgac gtaaatcggg tcgttaaacg cctccatata ttcgtgtgcg
    12961 atgtaataaa aattatgatt caatattttt tgaatcgccg gaagtttgcc gccgccgtcc
    13021 aagcccttgt cgttttctaa aatttccgcc agcgccttga gcgcgtccaa gcctttccgc
    13081 gtccgtgctt ccaagggttc ttcaagcaca tccctgccgg caaagtacat aatttcgcgc
    13141 aactgctcct gccgtttcca gccggggtaa acattgtatg aaatataggc aatgccgtgt
    13201 tcggtcaagt tgttccggca aatcgaaaaa attttgtctt taactgcgtc aggcacccac
    13261 gaccaaatgc cgtggacgat gatatagtca aacttcccga atgactcatc gacggtcaaa
    13321 atatcttttt cttccagacg cacatttttc aagcccattt tttcaatgat ggcgttgccc
    13381 tgcgcaacct gcctgccgga caagtcgata ccgacaaatt ccgcatccgg gtaataaaga
    13441 gcctgcgtga tgatgttgcc gcccatcgaa cagcccagct ccaagacctt ggcattggcg
    13501 gcgggcgcgg gctgcaaacc catcatgcgg gcgcgagcct ccaaggcgtt gatggcggtt
    13561 tgggaaaatg ctttggattc atacatcaaa tcatcatatg aatttttgat gttggacacg
    13621 tccggcacac cgttctccgt tgcagcacgc gcaaaggcgg cttttttctt tttgttcggc
    13681 atattttgtt tgtctgaagg cactattgcc cgcaagttta accaattcat cctacccgtt
    13741 caactaaatc aaatgccatc tgaaggcgcg gagcgtactt cagacggcat ctgggaggcg
    13801 cgaaggcttc agacg
//
LOCUS       DAWKQJ010000041        13532 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000041 DAWKQJ010000000
VERSION     DAWKQJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.041"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..79)
                     /locus_tag="WJL20_000901"
     CDS             complement(<1..79)
                     /locus_tag="WJL20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7683071.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WJL20_000902"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WJL20_000902"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ7683072.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WJL20_000903"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WJL20_000903"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ7683073.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLEDLR
                     GRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDGLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WJL20_000904"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WJL20_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ7683074.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDSLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /locus_tag="WJL20_000905"
     CDS             4798..5862
                     /locus_tag="WJL20_000905"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ7683075.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYIAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5882..6841
                     /locus_tag="WJL20_000906"
     CDS             5882..6841
                     /locus_tag="WJL20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ7683076.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQTITLASGE
                     FQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHG
                     KAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            6980..7657
                     /gene="tsaB"
                     /locus_tag="WJL20_000907"
     CDS             6980..7657
                     /gene="tsaB"
                     /locus_tag="WJL20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ7683077.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            7654..8094
                     /gene="rimI"
                     /locus_tag="WJL20_000908"
     CDS             7654..8094
                     /gene="rimI"
                     /locus_tag="WJL20_000908"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ7683078.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            8088..8825
                     /locus_tag="WJL20_000909"
     CDS             8088..8825
                     /locus_tag="WJL20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ7683079.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8887..9528
                     /gene="pyrE"
                     /locus_tag="WJL20_000910"
     CDS             8887..9528
                     /gene="pyrE"
                     /locus_tag="WJL20_000910"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ7683080.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9602..10036
                     /locus_tag="WJL20_000911"
     CDS             9602..10036
                     /locus_tag="WJL20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683081.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10033..11343
                     /gene="argA"
                     /locus_tag="WJL20_000912"
     CDS             10033..11343
                     /gene="argA"
                     /locus_tag="WJL20_000912"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ7683082.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11439..13454
                     /locus_tag="WJL20_000913"
     CDS             11439..13454
                     /locus_tag="WJL20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ7683083.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARTLS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
BASE COUNT         3026 a         4240 c         3611 g         2655 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctacctccc aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aatgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgt atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgctaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgatcaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa acccaaaccg tccaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcaggggc gaccatactg
     2041 ccaaaggcaa tgaatgcggt tttctcgcct tcgcggcgga tgatgccttt gccgatttca
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcagcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac gtcgaaatag cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaatacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcaggttgg cgacggcgtg gtatttgacg
     2701 gggtcgtttt cagcgagttt gtagccgttg ccctttttgg tgatgacgtg cagaagctgc
     2761 gggcctttgc gtccgcgcag gtcttccaat acatcgacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtatagcg gaagccgaag ttttcaaaca aagacagtga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttatgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggat
     3241 gtggaagaat gccctacgcc gaacgcgtcg tattccgact cgctgcgttt tgggaagcca
     3301 gctaaaccgc cgtattggcg catggtgtgc atctggtttt tgcgtccggt aaggattttg
     3361 tgcggatagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt gttgtacaca
     3421 tagtgcaggg cgatggtcag ctcgaccgca cctaagttgc tggcgaaatg accgccggtt
     3481 tgaccgacgg attccagcag aaaggcgcgc aactcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccggg tgcaacggcc aattatatat caagagtgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagcttgg tataaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gccctgatgt cgcgcgaagc ctgcaatagg
     3841 tgtccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     3961 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagttcgaac agcgcgtgat cgcgcaccgc cagcgagtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcggcag ggctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggtatg cagcagaccg
     4321 cgtttgacca gccaggcgca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ttgcgacaag
     4441 ccctgcggca cgccgccttc agcttcagac ggcatttgtg ccaacagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatgttt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aaccgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgagccg tttatggttt
     4741 tttgccgcaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 aacgcgcccg tcatcgtaca ggcgagtgca ggtgcgcgca aatatgcggg cgcgccgttt
     4981 ttgcgccacc tgattctggc ggctgtcgaa gaatttccgc acatccccgt cgtcatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttctcctcc
     5101 gtgatgatgg acggctcgct gctcgaagac ggcaaaaccc cttcttctta cgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgcct gcggcgtatc cgttgaaggc
     5221 gaaatcggcg tattgggcaa cctcgaaacc ggcgaagcag gcgaagaaga cggtgtaggc
     5281 gcagtgggca aactttccca cgaccaaatg ctgaccagcg tcgaagatgc cgtgcgcttc
     5341 gtcaaagaca ccggcgtgga tgcgctcgcc atcgccgtcg gcaccagcca tggcgcatac
     5401 aaattcaccc gtccgcccac aggcgacgta ttacgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca tcaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgtaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgatccg
     5701 cgcaaatatt tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacatc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgcg
     6001 tatgactagg agcaaacctg tgaaccgaac tgccttctgc tgcctttccc tgaccgccgc
     6061 cctgattctg accgcctgca gcagcggagg cggcggtgtc gccgccgaca tcggcgcggg
     6121 gcttgccgat gcactaaccg caccgctcga ccataaagac aaaagtttgc agtctttgac
     6181 gctggatcag tccgtcagga aaaacgagaa actgaagctg gcggcacaag gtgcggaaaa
     6241 aacttatgga aacggcgaca gcctcaatac gggcaaattg aagaacgaca aggtcagccg
     6301 cttcgacttt atccgtcaaa tcgaagtgga cggacaaacc atcacgctgg caagcggcga
     6361 atttcaaata tacaaacaga accactccgc cgtcgttgcc ctacagattg aaaaaatcaa
     6421 caaccccgac aaaatcgaca gcctgataaa ccaacgctcc ttccttgtca gcggtttggg
     6481 cggagaacat accgccttca accaactgcc tgacggcaaa gccgagtatc acggcaaagc
     6541 attcagctcc gacgacccga acggcaggct gcactactcc attgatttta ccaaaaaaca
     6601 gggttacggc agaatcgaac acctgaaaac gcccgagcag aatgtcgagc ttgcctccgc
     6661 cgaactcaaa gcagatgaaa aatcacacgc cgtcattttg ggcgacacgc gctacggcgg
     6721 cgaagaaaaa ggcacttacc acctcgccct tttcggcgac cgcgcccaag aaatcgccgg
     6781 ctcggcaacc gtgaagataa gggaaaaggt tcacgaaatc ggcatcgccg gcaaacagta
     6841 gcatttgaaa aatgccgtcc gaacacgata gtttaccgtt cggacggcat ttttgcgcca
     6901 tactgcccta ggacacgacg gcacggcagg caagccggtt tcactatata atgccgtctg
     6961 aaccaacgag aaccacacca tgcaagccga tttcaaccgc cccgtcctcg ccgtcgatac
     7021 cggaacttcc tatttgtcgc tcgcgctgcg cgccgacggc gaaaccctcc tgttccatca
     7081 ggaggccggc agccgccagt ccgaactgat cctgccggaa atccgcaccc tattccgaaa
     7141 tgcgggcatt accgccgccg atttgggggc gattgtttat gcgaaaggtc ccggcgcgtt
     7201 taccggactg cgtatcggca tcggcgtggc gcaaggtttg gcaacgccat tcaatacgcc
     7261 tttaatcggc atccccacgc tcgatgccgc cgcctcgctg ccgccgccgc aaagctgcat
     7321 actcgccgct gcggacgcgc gtatgggcga agtgttttat gcgtggttcg acacgctgaa
     7381 ccgccgccgt ttgagcgatt atcaggtcgg gcgggcggca gacatccggc tgccggaggg
     7441 atgtgccttt tcagacggca taggcagcgc gttcgcgttg gaagaaacgc cgccgttttc
     7501 aggcaaaccc gatatgccga ccgccgccga cttcctcaat ctggcggcgg aaggcggttt
     7561 tcccgccgtc gctgctgcac actccgaact gctctacgtc cgcaacaaaa tcgccctaac
     7621 cgccaaagaa caggcggaac ggaaagcccg aacatgaaca tccgccgcgc ctcaccgtcc
     7681 gactgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg gacgcaacgc
     7741 caatttgagt ccgcattggt ttcgccgtcc gaacaggttt tcctcgcgga aaaagacggc
     7801 gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact gcacctgatt
     7861 gccaccgcgc ccgaatgccg ccgccaagga attgcgtccg ccctgctcga atattggttc
     7921 gcacatctgc ccgaaggcac gcaacgcctg ctgctcgaag tccgcgcagg caacaccgcc
     7981 gcgcaggcac tgtacgcggc gcacggcttc agcattgcgg gcaggcggaa aaactattac
     8041 cgcgccaccg acgggcaaac cgaagatgcc gtcttaatgg agaaaatatg ttaagcgcgc
     8101 gctacctcca cctgcacgaa gctttgggtt tgggtccgat gtggctgaaa caggccgctg
     8161 ccgtcctgcc gcccaaaaac acacccgcaa ccccggcaca ggcacgtcct caaaccgtcc
     8221 gcgccgcccc gatccgccct tcccaacccc ataacggtca ggcgcggctc gaaacgatga
     8281 aagcgttgga aaccgccgcc gtacctacgc gcaaacccgc gcctgaaacc gaaacgcctc
     8341 tgcccggcct ttcagacggc atcgcccccg ttcccgccgc ttcgggcatc accaagcttg
     8401 ccgtcgtcag cctttgccca ccgatcgagg atgcggttta cgggcaactg ttccacggca
     8461 aagcgggtgt cctgcttgac aacatactca aagccgtagg actggatgcc gcctatgtcc
     8521 acaaaacctg ttgggtgaaa accgccgccg tcggcaaccc gatgccgtct gaagcggcaa
     8581 tcgaaaccgc gacggtttcc gtcatgcagg aactcgacgg ctgccgcgcc cccgccgtcc
     8641 tattcctcgg acaggctttt gtccagcctg aacggcaaac gatgattgaa actttgtgcg
     8701 gcagccgtcc cttcttcatc atcgaccatc ccgcccggct gttgcgccaa cccgaactta
     8761 aagcccgcgc ctggcaggtg ttgaaacagt tgaaacgcgc cttgcggcaa ggcggcggca
     8821 gttgaagcgc accgcacggg gcggtagaat cgcaactgcg tcccaatatc tgacagaaag
     8881 cacaaaatga ccgatttccg ccaagatttc ctcaaattct ccctcgccca aaatgttttg
     8941 aaattcggcg aatttaccac caaagccgga cggcggtcgc cctatttctt caatgccggc
     9001 ctctttaacg acggcttgtc cacgctgcaa ctggcaaaat tttacgcaca atccatcatt
     9061 gaaagcggca tccgattcga tatgctgttc ggccccgcct acaaaggcat tattttggcg
     9121 gcggcaaccg cgatgatgct ggcggaaaaa ggcgtgaacg tcccgtttgc ctacaaccgc
     9181 aaagaagcca aagaccacgg cgaaggcggc gtgttggtcg gtgcgccgct taaaggccgc
     9241 gtgctgatta tcgacgacgt gatttccgcc ggcacatccg tacgcgaatc gatcaagctg
     9301 attgaagcgg agggcgcaac ccccgccggt gtcgccatcg cgctcgatcg catggaaaaa
     9361 ggcacgggtg agttgagcgc ggttcaggaa gtggaaaaac aatacggtct gcccgtcgcc
     9421 cccatcgcca gcctgaacga tttgtttatt ctgttgcaaa acaaccccga attcggacag
     9481 ttcctcgaac ccgtccgagc ctaccgtcgg cagtacggcg tagaataaaa acaaagcata
     9541 tgccgtccga accgccttac gcctcagacg gcatcaaacc tgacacacac gaggaaatac
     9601 catgcccgcc tgtttctgcc cccactgcaa aacccgtctc tgggtcaaag aaacccaact
     9661 caatgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa ggactgttta aagccaaaga
     9721 ccatctggca agcacgaaag aacccatatt caacgatttg cccgaggctg tttcggatgt
     9781 caaactcgtt caccgtatcg gcacgcgcgc catcggcaag aaacagattt cccgtgacga
     9841 aatcgccggc atcctcaacg gcggcacaac ccagcccgat attccgcccg caaccgccgc
     9901 cacccctgct gccgcaccgc aggttaccgt accgcccgcc gcgcccgccc gtcaggatgg
     9961 gttcaactgg acgattgcaa ccctgtttgc ccttatcgtc ctcattatgc agctttccta
    10021 cctcgtcatc ctatgatcgt gcccgacctc tttgtcgccc acttccgcga agccgccccc
    10081 tacatccgcc aaatgcgcgg caaaacgctg gtcgccggca tagacgaccg cctgctcgaa
    10141 ggtgatacct taaacaagct cgccgccgac atcgggcttt tgtcgcaact gggcatcagg
    10201 ctcgtcctca tccacggcgc gcgccacttc ctcgaccgcc acgccgccgc gcaaggccgc
    10261 acgccgcatt attgccgggg cttgcgcgtt accgacgaaa cctcgctcga acaggcgcag
    10321 cagtttgccg gcaccgtccg cagccgtttt gaagccgcat tgtgcggcag cgtttccggg
    10381 ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact tcctgaccgc ccgtccgata
    10441 ggtgtgattg acggaaccga tatggaatac gcgggcgtta tccgcaaaac cgacaccgcc
    10501 gccctccgtt tccaactcga cgcgggcaat atcgtctggc tgccgccgct cggacattcc
    10561 tacagcggca agaccttcca tctcgatatg cttcaaaccg ccgcctccgt cgccgtctcg
    10621 cttcaggccg aaaaactcgt ttacctgacc ctttcagacg gcatttcccg ccccgacggc
    10681 acgctcgccg taaccctctc ggcacaggaa gcgcaatcgc tggcggaaca cgccggcggc
    10741 gaaacgcgac ggctgatttc gtccgccgtt gccgcgctcg aaggcggcgt gcatcgcgtc
    10801 caaatcctca acggtgccgc cgacggcagc ctgctgcaag aactcttcac tcgcaacggc
    10861 atcggcacgt ccattgccaa agaagccttc gtctccatcc ggcaggcgca cagcggcgac
    10921 atcccgcaca ttgccgccct catccgcccg ctggaagaac aaggcatcct gctgcaccgc
    10981 agccgcgaat acctcgaaaa ccacatttcc gaattttcca tcctcgaaca cgacggcaac
    11041 ctgtacggtt gcgccgccct aaaaaccttt accgaagccg attgcggcga aatcgcctgc
    11101 cttgccgtct cgccgcaggc acaggacggc ggctacggcg aacgcctgct tgcccacatt
    11161 atcgataagg cgcgcggcat aggcataagc aggctgttcg cactgtccac aaataccggc
    11221 gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag acgagttgcc cgaaacgcgg
    11281 cgcaaagact accgcagcaa cggacgaaac tcgcatattc tggtacgtcg cctgcaccgc
    11341 tgaccgcaac ggaaagccgc cgcagaaatg ccgtctgaac cccgtttcag acggcatttc
    11401 cccgattata aagacctgcc caaccctcaa ggaccccgat gaaatcctac cccgacccct
    11461 accgccattt tgaaaacctc gattccgccg aaacgcaaaa cttcgctgct gaagcgaatg
    11521 ccgaaacgcg cgcgcgtttt ttagaaaacg acaaggcgcg cacgctgtcg gacggcattt
    11581 tggcgcagat gcaggacacg cggcagattc cgttttgtca ggaacaccgc gcgcggatgt
    11641 accatttcca tcaggacgcg gagtatccga agggcgtgta ccgcgtgtgt gcggcggcga
    11701 cgtatcgttc cggctatccc gagtggaaaa tcctgttttc agtggcggat ttcgacgagc
    11761 tgctcggtga cgatgtatat ctaggcggcg tgtcgcacct ggtggaacag cccaaccgcg
    11821 cgttgttaac actgagcaaa tcgggcggcg ataccgcgta cacgctggaa gtggatttgg
    11881 aagcagggga gttggtagaa ggcggttttc actttccggc aggcaaaaac catgtgtcgt
    11941 ggcgcgatga aaacagcgtg tgggtgtgtc cggcctggga cgagcggcag ttgaccgaat
    12001 cgggctatcc gcgcgaggtg tggctggtgg aacgcggcaa aagtttcgag gaaagcctgc
    12061 cggtgtacca aatcggcgaa gacgggatga tggtgaacgc gtggcgttac ctcgatccgc
    12121 agggttcgcc gattgatttg attgaagcgt ctgacggttt ttacaccaaa acctatttgc
    12181 aggtgtcggc cgaaggcgag gcgaaaccgt taaacctgcc tgctgattgc gatgtggtcg
    12241 gctatctggc ggggcatctt ttgctgacgc tgcgcaagga ctggcaccgc gcgaaccaaa
    12301 gctatccgag cggcgcgctg gtagcggtga aactgaatcg cggcgaaatc ggggcggcgc
    12361 agcttttgtt tgcgcctgat gaaacgcagg cattggaaag cgtggaaacg accaagcgtt
    12421 ttgtcgtggc gagtctgctg gaaaacgtac aaggccgtct gaaagcgtgg cgttttaccg
    12481 acggcaaatg gcaggaagcc gaactgccgc gcctgccctc gggcgcgttg gaaatgaccg
    12541 accaaccgtg gggtggcgac gtggtttacc ttgccgccag cgatttcacc acgccgctga
    12601 cgctgtttgc gctggatttg aacgtgatgg aactgaccgt catgcgccgt cagccgcagc
    12661 agttcgattc agacggcatc aacgtgcagc agttttggac gacttcagct gacggcgaac
    12721 gcatccctta tttccacgtc ggcaaaaacg ccgcgcccga cacgccgacg ctggtttatg
    12781 cctacggcgg tttcggcgta cccgagctgc cgcattatct gggcagcatc ggcaaatact
    12841 ggctggaaga gggcaacgcc ttcgtgttgg cgaacatacg cggcggcggc gagttcggcc
    12901 cgcgctggca tcaggcggcg cagggaatca gcaaacacaa aagcgtcgat gatttgcttg
    12961 ccgtcgtgcg cgatttgtcc gagcgcggca tcagttcgcc ccaacacatc ggtttgcagg
    13021 gcggcagcaa cggcggcctg attaccgccg ccgccttcgt gcgcgaaccg caaagcatag
    13081 gcgcactggt gtgcgaagtg ccgctgaccg acatgatccg ttatccgctg ctctccgccg
    13141 gttcaagctg gacagacgaa tacggcaatc cgcaaaaata cgaagtctgc aaacgccggt
    13201 tgggcgaatt gtcgccgtat cacaatcttt cagacggcat cgattatccg cccgcgctca
    13261 tcaccaccag cctcagcgac gaccgcgtcc atcccgccca cgcgctcaag ttttacgcca
    13321 aactgcgcga aacctcgccg caatcttggc tctactcgcc tgacggcggc ggccataccg
    13381 gcaacggcac gcagcgcgaa gccgccgacg aactcgcctg cgtgttgctg tttttgaaag
    13441 agtttttggg ctaagggcgg ggagcggcac tgccgccgcg aatgaaaaaa ggtcgtctga
    13501 aactgctttt tcagacgacc ttttttaatg gt
//
LOCUS       DAWKQJ010000042        13378 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000042 DAWKQJ010000000
VERSION     DAWKQJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.042"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(100..528)
                     /locus_tag="WJL20_000914"
     CDS             complement(100..528)
                     /locus_tag="WJL20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ7683084.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(593..2341)
                     /locus_tag="WJL20_000915"
     CDS             complement(593..2341)
                     /locus_tag="WJL20_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ7683085.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2668..4101
                     /gene="ccoN"
                     /locus_tag="WJL20_000916"
     CDS             2668..4101
                     /gene="ccoN"
                     /locus_tag="WJL20_000916"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ7683086.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4128..4739
                     /gene="ccoO"
                     /locus_tag="WJL20_000917"
     CDS             4128..4739
                     /gene="ccoO"
                     /locus_tag="WJL20_000917"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ7683087.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4744..4914
                     /locus_tag="WJL20_000918"
     CDS             4744..4914
                     /locus_tag="WJL20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ7683088.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4938..6035
                     /gene="ccoP"
                     /locus_tag="WJL20_000919"
     CDS             4938..6035
                     /gene="ccoP"
                     /locus_tag="WJL20_000919"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ7683089.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6075..6269
                     /locus_tag="WJL20_000920"
     CDS             6075..6269
                     /locus_tag="WJL20_000920"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7683090.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6471..7880
                     /locus_tag="WJL20_000921"
     CDS             6471..7880
                     /locus_tag="WJL20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7683091.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPSGAAGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            7981..11190
                     /gene="recC"
                     /locus_tag="WJL20_000922"
     CDS             7981..11190
                     /gene="recC"
                     /locus_tag="WJL20_000922"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ7683092.1"
                     /translation="MFYLYQSNRLETLAALFAHIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEEETPVFEEGSRDTLLHALQTDIQNLDMPSEKAGNINTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWESAEPFEPQHADQIAKTYIEARREGRDFSQIAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSETETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(11260..12828)
                     /gene="mtrF"
                     /locus_tag="WJL20_000923"
     CDS             complement(11260..12828)
                     /gene="mtrF"
                     /locus_tag="WJL20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ7683093.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13162..13374
                     /locus_tag="WJL20_000924"
     CDS             13162..13374
                     /locus_tag="WJL20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683094.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3363 a         3219 c         3540 g         3256 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgttttc ggcttgtaat cagacgcggg cgttgtccag
      121 ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc
      181 aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc gcgtaccggt
      241 ccggcaatag ccaaggacgg gatattcggc ttgtccgatg agttggcgga aggtttcgac
      301 atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag taacgcctgc
      361 ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg
      421 gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat cggctttggt
      481 cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt tgtttccttt
      541 actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga
      601 acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta tccgctgccg
      661 ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag tggatattga
      721 tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt
      781 ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg ccgccgttga
      841 agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg
      901 ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca
      961 ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa tcttcggtac
     1021 tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc tgacagcgga
     1081 tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga tagagtttgt
     1141 ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc
     1201 acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg ctgttttcat
     1261 ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac
     1321 ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa taagattcgg
     1381 gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta atgccttcga
     1441 atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataaggggcg aacgcggcag
     1501 gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg
     1561 tggtgtaggc atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga
     1621 gcagggcggt gctgtcggcg tgttccaaac cgccgtaaat atggtcgccg acgtggagca
     1681 ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct gtttcgcaga
     1741 tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg
     1801 caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga
     1861 caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc cctgtttctg
     1921 gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag cggtgcggca
     1981 gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg
     2041 tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag cggatttgcc
     2101 actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg agcggcatgg
     2161 acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat
     2221 agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca tcttgtgtga
     2281 atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta taatgaatca
     2341 taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg cagcttaacc
     2401 tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt
     2461 gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc aaagggctcg
     2521 tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa atttagaaaa
     2581 aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga taaaaatctt
     2641 tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta caaagtggtg
     2701 cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt cggcgttatc
     2761 gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc ttggttccac
     2821 ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg cggttgcggc
     2881 ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct tttcggcggt
     2941 tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc
     3001 ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc gatcgatatt
     3061 ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat tgccaaacgt
     3121 aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt ggcggtcgca
     3181 cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc ataccccgtc
     3241 tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc cgtgggcttc
     3301 ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca agcagcccgc
     3361 cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt cacctatatg
     3421 tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca atctttgggt
     3481 atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa cggcatcatg
     3541 accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt cctgattgta
     3601 tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat taaaacggtc
     3661 aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc
     3721 tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct gttcggcaaa
     3781 gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac catcggcgtg
     3841 gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat gtggagttct
     3901 ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg caccatgcct
     3961 tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg cattatggcg
     4021 tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat ccctgcggtt
     4081 tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg aaattacaac
     4141 aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg
     4201 gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag ccggcgccgg
     4261 gcgtgaagcc ttacaatgcc ctgcaggttg ccggacgcga tatttacatc cgtgagggct
     4321 gttacaactg ccactcgcaa atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc
     4381 attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt tccaaacgta
     4441 ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac cgtatccacc
     4501 tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca tggcttgcac
     4561 gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta
     4621 ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac aaatccgagc
     4681 tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac
     4741 atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat ctgtttcctg
     4801 ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa tgccgcaaac
     4861 agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt
     4921 gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc tggaatatat
     4981 atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa
     5041 atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat gagtgggacg
     5101 gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt
     5161 ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac aaaggtctgc
     5221 tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat gagcaatacg
     5281 gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag
     5341 ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac ggctctgatg
     5401 ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc
     5461 ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct gcctggggtc
     5521 ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac
     5581 ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc agcggtccgc
     5641 ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt ttgggtccga
     5701 acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc gaaaccatta
     5761 ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa gacaaactgc
     5821 atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa
     5881 aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct gctcctgcag
     5941 aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa
     6001 aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt tgtgccgcct
     6061 gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat tgggccgacc
     6121 gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc aatacgatgc
     6181 ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg gttgactata
     6241 tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt atcggttatg
     6301 aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc ggttttaaac
     6361 gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt agtctttatt
     6421 gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta atggatacac
     6481 aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt tggcggtggc
     6541 atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt acgggtttgg
     6601 gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat gttgtgccgc
     6661 aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc gttaatccgg
     6721 aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt gccgtgttcc
     6781 gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg gcaaaagtgg
     6841 tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc cacagcgata
     6901 tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg acgattatca
     6961 tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag gcgatgctgc
     7021 tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg tttggaactt
     7081 gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca ggtatttggg
     7141 gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt gtcgaaccga
     7201 accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag gttaaggaag
     7261 tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg ggcaaagacg
     7321 gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg cgggaaatcg
     7381 gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta tggactttgt
     7441 cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg gtgcatatcg
     7501 accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac ccgttcggca
     7561 aatttatggc ggcaagcatt gcgctgcata tggggacttt gggctggtgg agcgtgttgg
     7621 cgaacgtctt gttctgcctt gccgtgattt ttatcggcat cagcggctgc gtgatgtggt
     7681 ggaaacgccg tccgtccgga gcggcgggca tggttccgcc ggcgcaaaaa atcaagctgc
     7741 ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc ccgaccgcgc
     7801 tgcttgccat tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg attcctgttt
     7861 tgaggaaatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca ttttgtttga
     7921 aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt tgtcgaaatc
     7981 atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcactgtt tgcccacatt
     8041 caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt gcagagtcag
     8101 gggatgcgcc gctacctcaa tacctgcctc gcccgcgatt tgggcgtggc ggcgaatttg
     8161 tcgttcagcc tgcccgccgg tctgacgtgg aagctgatga aaaaactgat tcccggcatt
     8221 ccggaactca gcccatttgc gcctgaagtg atgcgctggc ggctgttgga tttgttccgc
     8281 agcgaggcat ttcggaatac ggcagaattt gaagatgtga ggaatgtgct gcaagactat
     8341 ctgggcagcg gcgaatcggc agattatcag cttgcgggac agcttgcgga catattcgac
     8401 caataccttg tctatcgtcc gcagtggata gacgcgtggc agcagggcag gcggctcggt
     8461 ttgggcgacg atgaaatctg gcagtccaaa ctgtggcgtt acctcgacga tggcaggcag
     8521 agcgcgccgc accgtgtcgc gttgtgggaa aagctgttgg aatctttaag cagtgataag
     8581 ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta tttgcaactt
     8641 ttgcacaagc tgtccgaaca ttgcgacgtg ttcgtgttcg cactcaatcc gagcgggatg
     8701 tattggggca atgtcatcga agcggcgcaa atcctcaaag gcggcggcga tcccgattta
     8761 actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcggga ctttttcgac
     8821 tttttgaacg aaatggaaat agaagaagaa acgccggtgt ttgaagaagg cagccgcgat
     8881 acgcttttgc acgccctgca aaccgatatc caaaacttgg atatgccgtc tgaaaaagcg
     8941 ggaaatatta atacggacga cggatcgata cgcatcgtct ccgcacacag ccctttgcgc
     9001 gaattgcaga tactcaaaga caaactgttg aaaattctgc atgaacatcc cgattggcag
     9061 ccgcatgata tcgccgtatt aaccccgaat atcgaatcct atacgccttt tatcgaagcc
     9121 gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc agacgtgaaa
     9181 atcagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt gttggaaagc
     9241 cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt gcgccgtttc
     9301 ggactgactg aggacgattt gccgcttttg cacgatatgg ttgccgattt gaacgttcac
     9361 tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg gaagcaggcg
     9421 gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaacccgat gtggcaggat
     9481 gtcagcgcgt ggtatgccga cgtgaatcaa accgccctgt tcggacgttt tgccgccctc
     9541 ctcgaaaccc tgtcggatat tgtacggata tggcggcagc ccgcaacgat cggcgaatgg
     9601 gtggcgcgtt gccgggattt gcttgaaaca ttgttccaag ctgaagccga tgaccaaaag
     9661 tcagtccaaa accttgaaaa cgaatgggtc aaatggcagg cggaaacaga attggcgcaa
     9721 ttttccggac agttgccgca acaaaccgtc atccgccaca tccgccgctt tctcgacagc
     9781 gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt gccgatgcgg
     9841 agcctgccct ttaaagtcat ctgtctgttg ggtttgaacg acggagattt tccccgcaat
     9901 accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga ccgcgcccgc
     9961 cgcgacgacg accgctacct cttcctcgaa gccctcatca gcgcgcgtga aatcctgtac
    10021 ctgtcctaca tcgggcgcga catccgcaaa gacgaagaac tggctccgtc ttctttgttg
    10081 ggcgaactca tcgacacagt tgccgccatg accggcacaa acagccgcca acttgcacaa
    10141 aactggatag aacagcatcc gctgcaagcc ttctcgcgcc gatattttca agaaggcgga
    10201 cgttcagacg gcatattcgg cacgcgtacc gactacgccg ccgcgctcgg acaaacgccc
    10261 gaaccgccgc aacccttttt cgatcaaccc gtagaaaacg ccgaacctgt tgccgaaatt
    10321 ggacaggacg aatttatccg cttctggcgc aaccctgtca aagtatggct tcagcagcag
    10381 cttgcgtgga gcgaacccca tatcggcgaa gcctgggagt ctgccgaacc cttcgagccg
    10441 caacacgccg atcaaatcgc caaaacctac atcgaagcac gccgcgaagg acgggatttt
    10501 tcccaaatcg ccgcccgcat cggggcggaa agcctgctgc cgtcgggaga gttgggcaga
    10561 ctttggcagc aagacttcca aaccgctgcc aaacaaatcg acacggcggt tttaaacagc
    10621 cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct gaaaggcagc
    10681 ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc caacgcgccg
    10741 caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc gtctgaaacc
    10801 gaaacgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc ggaaatcgcg
    10861 caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat cgggcaaaac
    10921 cgcccgctgc cgttttttgc caaaacctcg cttgccgccg ccgaagcgtt tgcccaaaaa
    10981 caagattggg aagccgccct gaaaaaagcc caaaccgcct attacggcaa caaagtcagc
    11041 aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag ccaaaacccg
    11101 ctcgaacagc ccctgtttga aaacctcgtc cgcctgcttg ccgacacgtt tgccgcagcg
    11161 gaaaaaaagg aagaggccgg agcagcctga cgggatggaa aggcaaatgc cgtctgaaaa
    11221 cagcaaaaag gttttcagac ggcattttgt ttatcgtgtt taaggtgcgg gatagaatgt
    11281 gggcgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga ataaggcaat
    11341 ccacgcaatc aagaagaaag cggaatacgg caacatcata gaaatcagcg tacccacgcc
    11401 cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac tcatcatcgg
    11461 cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga cttcgggcgc
    11521 gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc attgcgcgga
    11581 ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga taaacaacac
    11641 gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa tatattgccc
    11701 aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga tgaccaaata
    11761 aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt cgccgcgcaa
    11821 acttcgggtt acccggccat aaacaatgcc cggcagcgca aacaacaaga aaataaaaac
    11881 aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat gacgcaaaat
    11941 accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa acaccacgcc
    12001 agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc gaatgtcttt
    12061 ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga ttttttcagt
    12121 aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa accagttggc
    12181 ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg tgatgcctgc
    12241 caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg aaacgccggc
    12301 gaaagccgca gccaaaccgg caagcggatg gcggccgagc gaatgaaaga tgacggcgga
    12361 caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa tccctgtaaa
    12421 aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc gcattaatgc
    12481 ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca acaccgttcc
    12541 caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga tcaaaccgtc
    12601 ggcattgagc aggctgacaa cgtaaatcaa accgtcatcg gcacgtcctt tcgcaccaac
    12661 agggcgcgga tcggggacgg atagtccgaa atacgcaccg acggcagagg caatcagcaa
    12721 taacacaatg aaaataataa aaagcgtaac cggatgcggc aacatattgc ccagccattc
    12781 gactgtgcgt aaaaatcgcc cgtcccgttg cgtatcggtt tgactcatct gcttctcctt
    12841 aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt ttgtaagcaa
    12901 actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat gcccacatcg
    12961 ttattctcat aaaggcggta atccgaaaac ttgacgttaa agccttatca ggaatgactg
    13021 gaactcaaaa aaacggattc ccgcctgcgc gggaatgacg ggattttcgg tttttgtttg
    13081 caagaatgcc ggagaagaat gcagtttaca ttttgaatgg tattgttatt aattgtaaat
    13141 aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaattatgga caaatatgat
    13201 ccctggcatg aagatgattt tgagtcgtat gaagacattg ccagagatgt atcgctgatg
    13261 acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga aaacggacat
    13321 tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat ttagtgaa
//
LOCUS       DAWKQJ010000043        13199 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000043 DAWKQJ010000000
VERSION     DAWKQJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.043"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            297..1610
                     /locus_tag="WJL20_000925"
     CDS             297..1610
                     /locus_tag="WJL20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ7683095.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1828..2003
                     /locus_tag="WJL20_000926"
                     /pseudo
     CDS             1828..2003
                     /locus_tag="WJL20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1999..3249
                     /locus_tag="WJL20_000927"
     CDS             1999..3249
                     /locus_tag="WJL20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7683096.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WJL20_000928"
     CDS             complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WJL20_000928"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ7683097.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4991..5248
                     /gene="grxC"
                     /locus_tag="WJL20_000929"
     CDS             4991..5248
                     /gene="grxC"
                     /locus_tag="WJL20_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ7683098.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5269..5712
                     /gene="secB"
                     /locus_tag="WJL20_000930"
     CDS             5269..5712
                     /gene="secB"
                     /locus_tag="WJL20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ7683099.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5798..7840
                     /gene="recG"
                     /locus_tag="WJL20_000931"
     CDS             5798..7840
                     /gene="recG"
                     /locus_tag="WJL20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ7683100.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLVVQIADGSGSVLFLRFIHFYAGHQKQ
                     MAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGSAAALRGDGTLTQALRHALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFANLEEDLHLLEQAREIAPLLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(8596..9639)
                     /locus_tag="WJL20_000932"
     CDS             complement(8596..9639)
                     /locus_tag="WJL20_000932"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ7683101.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(9636..9917)
                     /locus_tag="WJL20_000933"
                     /pseudo
     CDS             complement(9636..9917)
                     /locus_tag="WJL20_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10170..11324
                     /locus_tag="WJL20_000934"
     CDS             10170..11324
                     /locus_tag="WJL20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7683102.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATLLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            11553..11906
                     /locus_tag="WJL20_000935"
     CDS             11553..11906
                     /locus_tag="WJL20_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683103.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANVATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDSTNYC"
     gene            12306..12683
                     /locus_tag="WJL20_000936"
     CDS             12306..12683
                     /locus_tag="WJL20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary transporter"
                     /protein_id="HEZ7683104.1"
                     /translation="MQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQ
                     ELHISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRR
                     EEVGLLQEIRTQNLTLASLPQAA"
     gene            12748..>13199
                     /locus_tag="WJL20_000937"
     CDS             12748..>13199
                     /locus_tag="WJL20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7683105.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLY"
BASE COUNT         3079 a         3230 c         3697 g         3193 t
ORIGIN      
        1 atcccgcgtg tacggcacgc cccgcgctcc gcaatgatag gggcggtttc gtttgaatcg
       61 gcgcaccggg atgaatgccc ccgcgccgtc ccgataccga tttgccggtt gttccgtatc
      121 ggttgccgcc gtgaaatgac ggacggatgc aggaatggcg gcaggcgtga atgggaggaa
      181 atgaccgatt gacttgtttc aaaataaaaa agtagaatgc agggcttatc ccgcgtctgt
      241 gggcggtttt gcctgcacgg tcggttttct gtttaaaata aaataaagga gaaatgatgc
      301 tgacgtttat cggactgctg attatcgggg tcatcgtatg gttgctgctg acggaaaaag
      361 tgtcgcccat catcgcatta atcttggtgc cgctgtttgg ggcgttgctg gcggggtttg
      421 atgtatccca attaaaagaa ttttattcgg gcggcaccaa atcggtgatg cagattgtga
      481 ttatgtttat gttttccatt ttgttttttg gaatcatgaa cgatgtgggg ctgtttcgtc
      541 cgatgatagg cggtttgatt aagctgactc ggggtaatat cgtggcagtg agtgtgggga
      601 cggtcttggt gtcggtggtg gcgcagttgg acggggcggg tgcgacgacg tttttattgg
      661 tcgtccccgc ccttttgccg ctttacaagc gtctgcatat gaatccttac ctgctgtttt
      721 tgctgctgac ttccagtgcg ggattgatta accttctgcc gtggggcggg ccgaccgggc
      781 gggttgcaag cgtgttgggc gcagatgtgg gcgaattgta taaacctttg ttgacggtgc
      841 aaattatcgg tgtggtgttt atccttgcgc tgtccctgct tttgggtgtg cgtgaaaaaa
      901 ggcggattgt ccgggagttg ggcgcgttgc ccgccgtggc ggatttgata aagccggtgc
      961 ctttgtcgga agaagaacaa aaattggcgc gtccgaaact gttttggtgg aatgtcctgc
     1021 tgtttttggc ggcgatgagc ctgctttttt cgggcatctt cccgccgggt tatgtattta
     1081 tgctggctgc aacggcggcg ttgcttttga attaccgcag cccgcaggaa cagatggagc
     1141 ggatttatgc ccacgccggc ggcgcggtga tgatggcgtc cattattttg gcggcaggta
     1201 cgtttttggg gattttgaag ggtgcgggga tgttggacgc gatttccaaa gacattgtgc
     1261 atatcctgcc ggacgcgctg ctgccttatc tgcatattgc catcggtgtg ttgggcattc
     1321 cgcttgagtt ggttttgagt acggacgctt attatttcgg actgtttccg attgtggagc
     1381 agattacctc gcaggcgggc gtggcgcccg aagcagcagg ttatgcgatg ttgatcggca
     1441 gtatcgtcgg cacttttgtt acgccgcttt cgccggcttt gtggatgggc ttgggtttgg
     1501 cgaaattgtc gatgggcaaa cacatccgtt attcgttttt ttgggcgtgg ggtttgtcgc
     1561 tggcgatatt ggccagttcg atagcggcag gaatcgtgcc tctgccgtaa acggcgaagc
     1621 cgtctgaagc ctgaatcgtt cctgtggtct gtcgcaggct ctgcctgccc gtgtgttgca
     1681 atcgcgcccc aatgccgtct gaaggcagtt gccggcgcgg ttttttgtgc cgcagttttg
     1741 aaaaataccg gcaagcgtaa aggccgctaa attgcggcta attttggggt gttagaatag
     1801 gcacggttta ttttgaaggg aaagttgatg cgtgttttgc tggtggaaga cgatgcgatg
     1861 attgcggagg cggtgtcggc aagtttgaaa gacggcggct atgcggtgga ttgggtcaaa
     1921 aacggcgcgc ggctgccgct gcccagcctt atgacttgat gctgctggat ttgggtttgc
     1981 tggggcggga cggtttggat gtttgttccg ctggcaatgc ttgcaggtat gttttcctac
     2041 tacgaaacct tccacgaaac ggaagcgttg caggacgacc tgctccgtca ggcggcattg
     2101 tacgtcgcac ccgattccaa acccgaaact ttgcccgagg gcgacggcga tacgcgcatt
     2161 ttcgtgcaga tgccgcagca ggaagaccct gttgtcagcc tgcctgcgca tctggcggac
     2221 ggtctgcaca cgcttcaggc ggacggggac gacgattatt accgcgtcta tatccgcacg
     2281 accgaacagg gacggattgc cgtcatgcag gaaaacgaat accgtgaaga tttggcggcg
     2341 gatgcggcac ggcaaagcgt gttgcccctg ctggcggcac tgccgctgat gatactgctg
     2401 accgtgtgga ttacgcacaa agccatgcgc cccgtccgca aattgtcgca aagtctcgaa
     2461 caacgccgaa tcaatgacct gtctgccttg agtgtggaca atattcccag tgaaatcaga
     2521 gggttcgtaa ccgccatcaa cttgcttttg aaacgtgcgg atgaagacat ccgccaccgt
     2581 cagcggtttg tcgccgacgc ggcacacgaa ttgcgtacgc cgatgacagc cctttccctt
     2641 caggcggaac ggctcaacaa tatgtctctg ccacccgatg cggcgcggca gtctgccgtt
     2701 ttgcagcaaa gcatacggcg caacaaacac ctgctcgagc agcttttggc actggcgcgt
     2761 tcgcagtcgg acgaaacccc tttggcgaaa acgacattcg gtctgcaaag ccgtttccgc
     2821 caagtgttgc aggaactgat gccgcttgcc ttggaaaaac gtcaggacat tggtgtggcg
     2881 gtcggaggtg atgtcgaaat atttgccgat gaaactgaaa tctatacctt ggttaaaacg
     2941 tttgttgata acgcaatacg ttataccccg tccgaaggca ggatagattt gggtttcaca
     3001 gacgaaggga aatatcttgc cgtgtgggtg gaagataacg ggaacggcat tccggaatcc
     3061 gaacgcgtcc gcgtcctcga tccgttttac cgtattttgg gaacggagca gcaggggacg
     3121 gggctggggc tgtcgattgc cgacacgcta gccaaaaaat acggcggata tttggaactg
     3181 accgacagcc gcaggttcgg acacggtctg ctgatacgcg ccttgttgga caaggaaacc
     3241 ctgaaataga cagatgccgt ctgaaggctt cagacggcat tttttacatc agcacaatat
     3301 cgtattgttc ctgcgtgtaa gcggtttcga ccgccagaga aatcggtttg ccgatgaaat
     3361 ctatcagcat tgccaaggat tgcgattctt cgtccaaaaa caaatcgatg acgttggggg
     3421 cggcgaggat gcggaaactt tcggcatcgt aacggcgcgc ttcgcggacg atttcgcgct
     3481 ggatttcgta gcacacggtt tgcggcgttt tcagacggcc tctgccttgg caggaagggc
     3541 agggttcgca gaggacttgg tttaagtttt cgcgcgagcg tttgcgcgtc agctcgacca
     3601 gccccaggct ggtaaaaccg tgcagggtaa cgcgggtacg gtcgaaggcg agggctttgg
     3661 caagctcctg caacacggct tcgcggtggc tttcctgcgc catatcgatg aagtcgatga
     3721 tgatgatgcc gccgaggttg cgtagcctca attcgcgggc gatggtgtgg caggcttcga
     3781 ggttggtgcg gaagatggtt tcgtcgaagt tgcgtgcgcc gacgaagccg ccggtgttca
     3841 cgtctatcgt ggtcatggct tcggtggatt cgataatcag gtagctgccg aagttgaggt
     3901 tgacgcgcgg ttgcagggcg cggctgattt cctgttcgac gttgtgggtt tcaaacagcg
     3961 ggcgttcgcc tttgaacaat tctatcctgc ccaatgcgcc gtggacgtat tgttcggcaa
     4021 aacgcgtcat gcgcccgtgg tttacggtgg aatcgacgag gattttttgc gtgtcgcagc
     4081 cgaccatatc gcgcaatacg cgcaaactca agggcaaatc ctgataaagc agggtttccg
     4141 gcggccggat tttcgcctgt tcttggatgt gttcccacac tttggtcagg tagtcgatgt
     4201 cggactggag ctgttcgtcg gtggcgtttt cggcgttggt gcggatgatg tagccccggc
     4261 aggcattttc cggcaggagc ttgtcgaggc gttcgcgcag gctgctgcgt tcggcatcgt
     4321 cttcgatgcg ttgggacacg ccgatgtggt cttcttgcgg aagatggacg aggaaacgcc
     4381 ccgccagcga gatttgggtg gaaagccgcg cgcctttggt gttgatcggg tctttgatga
     4441 cctgcaccaa aacagactgc ccttcaaaca gcatatgttc gatgcgctgg gtttcttcgg
     4501 ggttgcggcg ttgttcgagg acatcgacga tgtgtaaaaa cgccgcgcgt tccaagccga
     4561 tgtcgataaa cgcgctctgc atcccgggca gcacgcggcg caccacgccc agatagatat
     4621 tgccgaccag gctgtgttcg ctgttgcgct cgatgtgcag ctcgcagata ttgttttcct
     4681 ccaacaccgc tacgcgcgtt tcctgcggcg tgatgttgac caatatcgtt tcgggcgggc
     4741 gcgcgatgtc tttggggatg gggagtcctg acaacatggt ttttcctgaa aatgtaataa
     4801 aaatattttt gcacccgttc cgccgcgcag cgcgggtgca aaaatatttt tcacaatgcc
     4861 tatcatactt taaaaaagaa actttgacac attccgcaca tgccgtttga acgtgcatct
     4921 gttccgcact tgccaaacag gggaaatgcc cctatattga ttatcattgc aaaactttcg
     4981 gaaacccaat atgcagaccg ttaccatgta cacaggtccg ttttgtccct actgcgcgat
     5041 ggcgaaaagg ctgctgcacg cggcaggtgt cggacatatc gacgaaatcc gtgtcgatgc
     5101 aagccccgaa gcctttgccg aaatgcagca gctttcggga cagcgcagcg tgccgcagat
     5161 tttcatcggc gaaacgcacg tcggcggatt taccgacctc taccgcctcc agcaggaagg
     5221 cgggctggac ggactgctga acccttaacc cccaactagg aaaacaaaat gagcgaagaa
     5281 ctgcaacccg tattcagcat cgagcgactg tatgtcaaag acttgtcttt ggaagtgccg
     5341 cacgcgccgc aaatcttttt ggaacagggc gagcccgaag tggagatgcg cgtttccacc
     5401 ggcagccaaa agctggaaga cggctactac aacgtggatg taaccgttac cgtaaccgcc
     5461 aaattggata acgagcgcac gatgtttttg aacgaagtaa cccaaagcgg cattttccgt
     5521 ttggaaaaca tccccgaaga agatgtgcag ctgctgttgg gcgtggcgtg tccgaacatc
     5581 ctcttccctt acgcgcgcga agccgtttcc agtacggtaa cgcgcgccgg cttcccgccc
     5641 gtcctgctcg cgccgattaa ttttgaggcg atttaccaac aacagcagga agccgaagcc
     5701 gccggggctt gattcctgcc tgtcgatgcc gtctgaatcc gtttcagacg gcatttttgt
     5761 tttcggataa aataggcgcg tccaaaggaa tctgcctatg atgtcgcccg aaacccgaaa
     5821 acagctcaaa atcaccgatg tttccgccaa gaagctcgac aaactcaacc ttcataccgc
     5881 gtgggatttg gttttgcatc tgccgctgcg ttacgaggac gagacgcaca ttatgccgat
     5941 taaggacgcg ccgatcggcg tgccgtgtca ggtcgagggg gaggttatcc atcaggaagt
     6001 aacgttcaaa ccgcgcaagc aacttgtcgt ccaaatcgcc gacggctccg gcagcgtcct
     6061 ttttctgcgc ttcatccact tttacgccgg ccatcaaaaa caaatggcgg tcggcaaacg
     6121 catccgcgcc gtgggcgaaa tcaaacacgg cttttacggc gacgagatga ttcatcccaa
     6181 aatccgcgat gccgagggcg gcggtttggc ggaaagcctc acgccggttt acccgaccgt
     6241 aaacggtttg aaccagccca ctttgcgccg tattattcag acggcgttgg acgttacgcc
     6301 gctgcacgac acgctgcccg atgccctgct gtgccgtctg aagctgccac acctcgccga
     6361 aagcctgcgt cttttgcatt cgccgccgcc gagtttcacg attcatcagc tttcagacgg
     6421 cacgttgcct gcatggcaac ggctcaaatt cgacgaactt ttggcgcaac agctttcgat
     6481 gcggctggcg cggcagaagc gtatcggcgg ctcggcggcg gcattgcgcg gcgacggcac
     6541 gttgacccaa gccctgcgtc acgctttgcc gtttgccctg accgatgcac aagaaaaagt
     6601 tgtttccgaa atctgccgcg atatggcgca aacctacccg atgcaccgcc tgctgcaggg
     6661 cgatgtcggc agcggcaaaa ccattgtcgc cgccttgtcc gcgctgaccg ccatcgaatc
     6721 cggtgcgcag gtggctgtaa tggcgcccac tgaaatcctt gccgaacagc attttattaa
     6781 atttaaacaa tggctcgaac ctttgggcat tgaagttgtc cgcctttttg gcagtttgcg
     6841 taaaaaagcc aaagacgaag ccaaagccaa actcgccgac ggcagcgtca aaatcgccgt
     6901 cggcacgcac gccctgtttt cagacggcgt ggcgtttcac aatttgggct tgaccattgt
     6961 ggacgaacag caccgtttcg gcgttgccca acgcctcgcc ctcaaaaaca aagggcgcga
     7021 agtccatcag ctgatgatgt ccgccacgcc catcccgcgc acgcttgcga tgagtttttt
     7081 cgccgatttg gacgtgtccg tcatcgacga attgccgccc gggcgcacac cgattaaaac
     7141 gcgcctcgtc aacaacgtcc gccgcgccga agtcgaaggc ttcgtcctcg gcacttgccg
     7201 aaaagggcgg caggcgtatt gggtctgccc attgattgaa gagagcgaaa ccctgcaact
     7261 gcaaactgct accgaaaccc tagagcagct tcagacggcc ttacccgaac tcaacatcgg
     7321 cttggtacac gggcgcatga aagccgccga aaaggccgaa gtgatggcgc ggttttcttc
     7381 gggggggctg aacgtcttgg tcgccaccac cgtcatcgaa gtcggcgtag atgtgcccaa
     7441 tgccgccctg atggtcatcg aacacgccga gcgcatgggc ttggcgcagc tgcaccaatt
     7501 acgcggtcgg gtagggcgcg gagcggcaga aagcgtgtgc gttctcctgt ttgccgagcc
     7561 attgagcgaa ctcgccaaag cgcggttgaa agtcatctac gaacacaccg acggctttga
     7621 aatcgcccgc caagacctca acatccgcgg ccccggcgaa tttctcggtg cgcgtcaaag
     7681 cggcgttccc atgctgcgtt ttgccaatct tgaagaagac ttacaccttt tggaacaagc
     7741 gcgcgaaatc gcccctttgc tgattgaaca aaaccctgaa atcgtcgaag cccatttggc
     7801 acgatggctt tccggcaggg aaggttattt gggcgtgtga gcgaaatgtc gccggaaata
     7861 ttggaaaaaa gtgcaacgga atctgatttt gcattatttt tgacacattt tcatgatttt
     7921 aaatctcgtt attcccaagt gggcggaaat ccggcaatgg gaagccgcag agacttatcg
     7981 ggaaaacggc aagccctccg ccgtcattcc cgcgcaggcg ggaatctaga aatgaaaaac
     8041 cacggtgtta tcggaaatga ttgaaaccgg agaaatgacg gaatcgaagc gtgtggaaat
     8101 gtgtcggaaa ttgctgaagc tggataaact agattcccgc ttttgcggga atgacgggat
     8161 tttaggtttt tggtttgttt tcgcggcaat gatgggattt taggtttttg gtttgttttc
     8221 acggcaatga tgggatttta ggtttttggt ttgttttcac ggcaatgatg ggattttaaa
     8281 ttgcaggtat ttaccgggaa aaacggcaag ccctccgccg tcattcccgc gcaggcggga
     8341 atctagaaat gaaaaaccac ggtgttatcg gcaatgactg aaaccggaga aatgacgaaa
     8401 ttgaagcgtg cggaaatgtg tcggaaatcg ctgaagccgg ataaactaga ttcccgcttt
     8461 tgcgggaatg acgggatttt aggtttttgt tttcgcggaa taatgagcca aaagccgtct
     8521 gaacctaaaa aaccgccgct ttcagatggc atcaaaaaac cgcaggcacg atgcctgcgg
     8581 tttgccgttt gcgtttcagg ggagcagggg gattgcgtcc aagccgtgtg tttccttcag
     8641 tccgaacatg atgttcatat tttgcaccgc ctgtcccgcc gcgcctttga cgaggttgtc
     8701 gatgacggaa aggacgaccc acacatcgga ttgcgccgcc tgttggatgc tgatgcggca
     8761 gaggtttgcg ccgcgcacgc tgcgggtttc gggtgtcgaa ccggcgggca ggatgtccac
     8821 gaacaggctg tcgcggtaat agtcgcgcag gatggtttcg gggcagatgc cgtctgaaag
     8881 gtggaggtaa acggtggcgt gcataccgcg tatcatcggc gcgaggtgcg gcgtgaacac
     8941 gaatccttcg gcgatgccgt cctgaagccc ggcgatggtc tgcctgattt cgggcaggtg
     9001 gcggtgtcca gctatgccgt aggctttgaa gttgtcgccg acttcgcaca acagcgaacc
     9061 gacattgcct ttcctgcccg cgccggacac gccggatttg cagtcggcaa tcagcggcat
     9121 accgggcttc agacggcatt gccgcaacag cggcacgagc ggcagggata cgcaggtcgg
     9181 gtagcagccg gggttggcga cgaggcgcgc ctgtgcgacg gcttcgcggt tgagttcgct
     9241 caatccgtac acggcttggg aaacgaggcc gggggcggcg tgggtcatgc cgtaccagtg
     9301 ttcccaggtc ggaatgtccc atatgcggaa gtcggcggaa aggtcgatga cgcgcacgcc
     9361 ctgttcaatc aggcgcggcg cgtctttcat ggcgatgccg ttgggcgtgg cgaagaagac
     9421 gatgtcgcat tgttccaaac ctgcctcgtc gggcgtttgg aaggcgaggc cgtacacgcc
     9481 gcgcaaactc ggaaagtaat cggcaactgc ggttcccgct tcgctgcggc tggttacggc
     9541 ggcgacttcg acatcgggat gggcggcaag caggcgcagc agttccacgc cggtgtagcc
     9601 cgtcgcgccg acaatgccga ctttgatttt tttactcatg gtttttcctt tgtgtggttg
     9661 gcgggtatgc cgtccgaacg ctgtccgacg gggttttgat tggcggcgga aggacggtac
     9721 acaaagcgtt tgaaaagcgt gtctaattta atggcatccg cctctgtttt taaaacctag
     9781 ccctgccgtc cggaataaac ataaccgtag gcgcgaaagc cggcaggata tttcggtgcc
     9841 tgccccaaac ccaaaccgta tgcggcgaaa gcggtggtcg tggagaagga aagcaggagg
     9901 aggacgaggg ttttcataag gttccaaacc gaacggggcg atgaagcccg attatagcaa
     9961 aaggtcgtct gaaaaccgtt ttcagacgac cttttattta attacaataa aaatgttaac
    10021 aacaaaaaac aaaccgcttt tttccgtttg gagacaaatt tttaactaaa attgtcaaca
    10081 attccttgat gcacacaaaa aaaaacttta atatttgtaa atatatgtaa cattataaat
    10141 tttaaatatt ttctgagtaa ggggaagtaa tgagcatcca tctcgacttc ggcattagtc
    10201 ctaaaacgtt ccgacagact tatctgtatc aaaagcccaa gctctttaaa ggagcggttc
    10261 ggaatctcga atccgcatct tggaaagata tcaacgagat ataccaacga gcagacccaa
    10321 ccgcaccgct gtttcatctg cgtaaaaaag gcgcaatcgt tcctaaagaa gaatacgtcg
    10381 aaagtttcga cgatttgggc aaaactcgct accgttttat taaatccgtt atctacgaac
    10441 atatgaagaa tggtgcgtcg ttagtctata accatattaa caacgagccg ttttcagatc
    10501 atatttcccg tcaagtcgcc cgctttgctg gcgcacatac tattgttagt ggatatcttg
    10561 cttttggcag cgacgaatct tataaaaacc attgggacac ccgcgatgtg tatgccatcc
    10621 agcttttcgg caagaaacgt tggcaactta ctgcccctga tttccctatg ccattgtata
    10681 tgcaacagac taaagatact gatatttcca ttcctgaaca tatcgatatg gatattatcc
    10741 ttgaagcagg tgatgtcctc tacatcccgc gcggttggtg gcacagacct atcccgctcg
    10801 gctgtgaaac cttccacctc gctgtcggta cctttccgcc caacggctat aattacctcg
    10861 agtggctaat gaagaaattc cccacgatag aaagtctgcg ccacagtttc tcagactggg
    10921 agcaagatag gacgcgtatc aacgatactg ccgcacaaat tgctgccatg attgccgacc
    10981 ccgtcaatta cgaagccttc agtgaagact tcctcggcaa agaacgtacc gataccgctt
    11041 ttcatctcga acagttcgcg aatcccaacg ctactctgct ttcagacgac gtcaggttga
    11101 gactaaatgc caataatttg gatacgttgg aaaaggggta tttgattggg aatgggatga
    11161 agataagcgt agatgaattg gggaaaaaag ttttagaaca catcggtaag aatgaaccgt
    11221 tattgttgaa aaatctactg gttaacttca atcagggaaa acatgaagaa gttaggaagt
    11281 tgatttatca gttgatagag ttagattttc tggaactttt gtgagggatt ctatgaaaaa
    11341 ttggaagcaa tttacatttt tcgtaatatt agtaatagct tgttatcaat tgctatattt
    11401 tttatcagat atgtttcttc tcgactacat taacaaatat agttggaatt tgaattttat
    11461 tcagggtact ttgaattttt tttcaatata tctgccatat gtttttgtta gccgaatttt
    11521 taggaatacc aaccaagaaa aggagtataa aaatgattga acttcaactt catgaattga
    11581 agctggtttc agggggaggt cctgtaacag acaatatagc tggaaatgta gctaatgttg
    11641 ccacaaccaa aggaggtccc acatgggggg atttggttgc aatacctgct gccgcagcgg
    11701 cagtttatta tctgccgaaa aatgcttacg gtgctgctgg tgcaaatggt gtatacaatg
    11761 tgactcgtaa ttgggtaaat gatgccgtta atgcacctcc ttataacgga agaccaatct
    11821 ttgagattga acatggatta actgctcccg caacaaaagc agataaatca ggaaacggct
    11881 acactgacag cacaaattac tgctgatatt tctcatcgtc cagacaatct ctaggggtcg
    11941 tctgaaactt tcttaacttc aattttatga atagacccaa gtaacccttc ttccgtcccg
    12001 aagtcgccgt tgcccgccaa accagcctga cgggtaaagt gattctgaca cgaccgttgt
    12061 cattttccct atggacgaca tttgcatcga tatctgcgtt attgattatc ctgtttttga
    12121 tatttggtaa ctatacgcga aagacaacag tggagggaca aattttacct gcatcgggcg
    12181 taatcagggt gtatgcaccg gatacgggga caattacagc gaaattcgtg gaagatggag
    12241 aaaaggttaa ggctggcgac aagctatttg cgctttcgac ctcacgtttc ggcgcaggag
    12301 gtagcgtgca gcagcagttg aaaacggagg cagttttgaa gaaaacgttg gcagaacagg
    12361 aactgggtcg tctgaagctg atacacggga atgaaacgcg cagccttaaa gcaactgtcg
    12421 aacgtttgga aaaccaggaa ctccatattt cgcaacagat agacggtcag aaaaggcgca
    12481 ttagacttgc ggaagaaatg ttgcagaaat atcgtttcct atccgccaat gatgcagtgc
    12541 caaaacaaga aatgatgaat gtcaaggcag agcttttaga gcagaaagcc aaacttgatg
    12601 cctaccgccg agaagaagtc gggctgcttc aggaaatccg cacgcagaat ctgacattgg
    12661 ccagcctccc ccaagcggca tgagacagaa caaagctggc ttgaacgcac cattgccgat
    12721 atttctcaag aagttttgga ttttgaaatg cgctctgaac aaatcatccg tgcaggacgg
    12781 tcgggttata tagcaatacc gaacgtcgaa gtcggacagc aggttgatcc ttccaaactg
    12841 ctcttgagca ttgttcccga acgtaccgag ctatatgccc atctatatat ccccagcagt
    12901 gcagcaggct ttatcaagcc gaaagacaag gttgtcctac gttatcaggc atatccctat
    12961 caaaaattcg ggcttgcttc cggcagtgtc gtatcagtag caaaaacggc actgggcaga
    13021 caggaattgt cgggattggg catggtatcc tccgatttgg cgaagagcaa cgaacctgtt
    13081 tatctcgtga aaataaaacc cgacaaacca accatcactg catacggtga ggaaaaaccg
    13141 ctgcaaatcg gcatgacgtt ggaagcagac atcctacacg agaaacggcg attgtacga
//
LOCUS       DAWKQJ010000044        12913 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000044 DAWKQJ010000000
VERSION     DAWKQJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.044"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..325
                     /locus_tag="WJL20_000938"
     CDS             <1..325
                     /locus_tag="WJL20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683106.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..974)
                     /gene="gloA"
                     /locus_tag="WJL20_000939"
     CDS             complement(498..974)
                     /gene="gloA"
                     /locus_tag="WJL20_000939"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ7683107.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(938..2107)
                     /gene="lapB"
                     /locus_tag="WJL20_000940"
     CDS             complement(938..2107)
                     /gene="lapB"
                     /locus_tag="WJL20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ7683108.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2118..2432)
                     /locus_tag="WJL20_000941"
     CDS             complement(2118..2432)
                     /locus_tag="WJL20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ7683109.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2742..3740
                     /gene="ilvE"
                     /locus_tag="WJL20_000942"
     CDS             2742..3740
                     /gene="ilvE"
                     /locus_tag="WJL20_000942"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ7683110.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="WJL20_000943"
     CDS             complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="WJL20_000943"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ7683111.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="WJL20_000944"
     CDS             complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="WJL20_000944"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ7683112.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5755..5985)
                     /locus_tag="WJL20_000945"
     CDS             complement(5755..5985)
                     /locus_tag="WJL20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683113.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAMPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(5955..7598)
                     /gene="pgi"
                     /locus_tag="WJL20_000946"
     CDS             complement(5955..7598)
                     /gene="pgi"
                     /locus_tag="WJL20_000946"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7683114.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8108..9340
                     /gene="pilG"
                     /locus_tag="WJL20_000947"
     CDS             8108..9340
                     /gene="pilG"
                     /locus_tag="WJL20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ7683115.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9414..10274
                     /locus_tag="WJL20_000948"
     CDS             9414..10274
                     /locus_tag="WJL20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ7683116.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10276..10908
                     /locus_tag="WJL20_000949"
     CDS             10276..10908
                     /locus_tag="WJL20_000949"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ7683117.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESMMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            10901..11110
                     /locus_tag="WJL20_000950"
     CDS             10901..11110
                     /locus_tag="WJL20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ7683118.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11195..12871)
                     /gene="pilB"
                     /locus_tag="WJL20_000951"
     CDS             complement(11195..12871)
                     /gene="pilB"
                     /locus_tag="WJL20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ7683119.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLVGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2962 a         3591 c         3384 g         2976 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggccgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcacc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc gtcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcatttttgt gttcctctcg gtagtggtta aacttcgatt ttgtttggcg
      961 taaacgtctg ccatttgttg caggcaggac aatgccagaa aaagacttgt gatttgaagt
     1021 ggcagtttcg gcaccggtac atcacgctgc gctgtagctg ccgtccgata accgaacgca
     1081 tcatatcggc atcggctttc caagccggat ccaaatcgct gagtttcaaa ccaagcaggc
     1141 ggtacacgcc gttgaggtcg ggcttgcggc ggacaagctc gacggcggtt tgcgcggctt
     1201 ctttctcgca cttaagcagc agggatttct cgtacacgac attgatcagg tcaagttcgg
     1261 gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt ccctgcgcgg
     1321 cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt tgctgctcga
     1381 tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttcg atgtcgccca
     1441 aaatcatgtt ggcgcgggtg cattttttgt tggcttcgag tgccttgccg acattgaaac
     1501 gcgcggcatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa tagaactgtg
     1561 caatctcaaa ctgataggtc tgctcgtcat ggctgagcag ccgggcggtt tcaaccgctt
     1621 tttcccaatc cctgtcctgt tggtagatat tgagcaggtg ctgtctggct tcacgcgcca
     1681 tttcaccgtc ttgcagcccc aaaaaaatct gttcggcacg gtcaaccaac cccgcacttt
     1741 gatagttttg cgccaattca aataatacgc gcgcacgctt ggctccggtt gtatcgggag
     1801 agtcaagcaa tgtttggtgc atattgatgg ctttgtcgtt ttcgccacgc tgacggtaaa
     1861 gcttgcctag ggtgaggttc aggtcgtatg actgaggctg ctgatcgatg acctctgcca
     1921 attccctggc ggcacgtccg ctgttgcggt caaccaaggc atccagactt ttataaaatc
     1981 ccgacggtat gctttttgcc tgctttaata cagtcttcat atccacacgg gcggcaaacc
     2041 agcccatcgc gaagaaaacg ggcaaaagga taatcggcag caggataatc cacaattcat
     2101 tgtccatatc ggctttctca aggctgtttg gcagattcgg gcgcattttg cgccggtggt
     2161 gcggtcagct ctttccccgt caaacgcgca tttttcttta cttcggcacg caacctgccg
     2221 ttctcgccac gtaacgacaa caaccgtccg aacaaggcaa acattccaaa aataataccg
     2281 actacaaatg cgccgaacaa tacgacaatc agcggcaaat cgaatttttg ccccggcagg
     2341 taggagaagg taacggcatc cgtattaatg acggcaagca gcaggaagaa cagcaggata
     2401 atgattttga cgacggtata gataagtttc atgtttgctc cgtaaataaa aacgccgccc
     2461 ggacagcttc gcactctgaa ccaagattgc gctagtttaa acgatttgca cggtttcgga
     2521 taggatgcgg cagcgtgttc ggacgacata cggagtatgc gattgccgac aattttacca
     2581 aatacaccgt tcctttccat ttgaaaaata acggattgga caccgcatcg acagaaaaac
     2641 ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaatctt caaattcaaa
     2701 ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga cccgtacccg
     2761 ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc gaaggcaaat
     2821 ggcagccgac cgaatggcaa tcttcccaag acctctccct cgcaccgggc gcgcacgccc
     2881 tgcactacgg cagcgaatgt ttcgagggac tgaaagcctt ccgtcaggca gacggtaaaa
     2941 tcgtactgtt ccgtccgact gccaatatcg cgcgtatgcg gcaaagtgcg gacattttgc
     3001 acctgccccg ccccgaaacc caagcctatc tcgatgcttt ggtcgaactg gtcaaacgtg
     3061 ccgccgacga aattcccgat gcgcctgccg ccctgtacct gcgcccgacc ttaatcggta
     3121 ccgatcccgt catcggcaag gccggttctc cttccgaaac cgccctgctg tatattttgg
     3181 cttcccccgt cggcgactat ttcaaagtcg gctcgcccgt caaaatttta gtggaaaccg
     3241 aacacatccg ctgcgccccg catatgggcc gcgtcaaatg cggcggcaac tacgcttccg
     3301 ccatgcactg ggtgctgaag gcgaaagccg aatacggcgc aaaccaagtg ctgttctgcc
     3361 cgaacggcga cgtgcaggaa accggcgcgt ccaactttat cctgattaac ggcgatgaaa
     3421 tcattaccaa accgctgacc gacgaatttt tgcacggcgt aacccgcgat tccgtactga
     3481 cggttgccaa agatttgggc tataccgtca gcgaacgcaa tttcacggtt gacgaactca
     3541 aagctgcggt ggaaaacggt gcggaggcga ttctgaccgg tacggcagcc gtcatctcgc
     3601 ccgttacttc cttcgtcatc ggcggcaaag aaatcgaagt gaaaagccaa gaacgcggct
     3661 atgccatccg taaggcgatt accgacatcc agtatggttt ggcggaagac aaatacggct
     3721 ggctggttga agtgtgctga tgctttaaat aaaaaatgcc gtctgaaacc cgtttggcgt
     3781 ttcagacggc attttcgcat ccgaaccgtt tccgctgcac ctgcagcaag tcggcacaaa
     3841 ggcaatcggt taaaacaagc gtccgcattt cccatcccgc ctgccgtaag tcgggcattt
     3901 ccctagaaat acgcttcaga cggcaaaacg ccgcccgaaa ccgatatgcg gcacggacgg
     3961 cgcacggatt tgaaaacggc agattatccc tcggtgctca aggcattgat gctgtaaccg
     4021 ccgtcaacgt aagtgatttc gccggtaatg ccggacgaca ggtcggacag caggaaggcg
     4081 gcggtattgc cgacttcttc gatggtaacg ttgcggcgga gcggattgtg ggcggcgacg
     4141 tgtcccaaga gtttgccgaa atcggcgatg ccggaggcgg caagcgtttt gatcgggcct
     4201 gcggaaatgc cgttgcagcg gatgccttct ttgcccagac aggcggcggt aaaacggatg
     4261 cccgcctcaa ggctggcttt tgccataccc atcacgttgt aattcggaat cgcgcgcacc
     4321 gcgcccaagt agctcagggc aacgatggcg gagtttctgc cgcgcatcat cggacgggcg
     4381 gcttttgcca atgcgggcag gctgtatgcg gaaatttcgt gtgcggtgtt gaacgcttcg
     4441 cggctgatgc tgtcgaggaa gtcgccgctc aaggcttctt tcggcgcaaa accgatggaa
     4501 tgcaccaaac cgtccaagcc gtcccaatgt ttgcccaaat cggcgaacac ttggttgatt
     4561 tcgtcgtcgc tggcgacatc gcagcggaac acgagttcgg aatccaattc cgccgccatt
     4621 ttgcggacgc gctcttccag tttgtccaca acgtaggtaa acgccagttc cgcgccttgt
     4681 tcgcggcagg ctttggcgat gccataagcg atggaacgct cggaaatcat gccggtaatc
     4741 agaatttttt tgccttgcag aaaacccatt ttgtatcctt caaatagtgt tcggttcatt
     4801 ctaaaccgtt gattatagca aattgtccct gtttctgtgt tttcacgttg cagcatgcaa
     4861 acggcaatgc cgtccgaagc ggatttcaga cggcatcgga cgtttcaaat acggtttaag
     4921 gcatcagatg ccgcgcaaca attcgttcac gctggttttc gcacgggttt gcgcgtccac
     4981 gcgtttgacg atgacggcgc agtaaaggct gtggctgccg tctttggaag gcatactgcc
     5041 ggacacgacg accgaaccgg ccggtacgcg accttggtag atttcgccgg tggtgcggtc
     5101 gaagattttg gtggattgac cgatgaacac gcccatagaa atcacgctgc cttcttcgac
     5161 aatcacgcct tcgacgattt cggaacgcgc accgatgaag cagttgtctt caatgatggt
     5221 gggtgcagcc tgcaggggtt cgagtacgcc gccgatgccg acaccgccgc tcaagtggac
     5281 gtttttgccg atttgcgcgc aagagccgac ggttgcccaa gtatcgacca tcgcgccttc
     5341 gtcgacgtat gcgccgatgt tgacataaga cggcatcagc acgacatttt tcgccacaaa
     5401 gctgccgcgt cgggcaaccg cacccggaac ggcgcggaag cctgcgtttt tgaactcgtc
     5461 ttcagaccag tcggcaaact tggtcggcac tttgtcgaag tatttgttca cgccgtcgtt
     5521 gaggacttcg ttgtcttgga tgcggaagga cagcaacacg gcttttttcg cccattcgtt
     5581 gactttccac tcacccacgc ccaaacgttc ggcaacgcgc agtttgccgg aatcgagttg
     5641 gcggatggtt tccaacacgg cttctttgac ttcgggagta acggtggtcg gggtgatgtc
     5701 cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa gacataatgt ttccttatgt
     5761 gagatgtttc gtgggacgct tgccggtatt ttcaagcagt atgttcattt gaaatgaatg
     5821 cgctattgtg ccacgcaaac gcatattcgt cagcaatacg cgggcggttg ccgaacattg
     5881 gcgtttcgga atcgggcggg cggcaggcat tgccttttcc gcacccgaat caatacagaa
     5941 cggcggcggc acttttatgc cgcgccgccg cctttcagac ggcattcgcg gtaaaacgcc
     6001 atcagccctt cggtcgaggc atcgtgtacg gacgtgccgc cttccagttc gccgatgatg
     6061 gttttcgcca actgtttgcc gtactcaacc ccccactgat cgaaggggtt gacgttccat
     6121 atcgcgcctt ggacgaaggt tttgtgttcg taagccgcca tcagcatacc cagattgtag
     6181 ggcgtgaggc ggtcgagcag gatgctgttg ctggggcggt tgccggggaa ttctttgtgc
     6241 ggcgcgaggc gttcgcgttc cgcttcgggc aaatctgcca gttcggcgcg tgcttcgtcc
     6301 aaggttttgc ccttcatcag ggcttccgct tgggcaaagg cgttggcaac ggtaaaacgg
     6361 ctgcgtccgt cctccacgcc ctgcgccgtc atcgggacga taaagtcgca gggaatcagg
     6421 cgcgtgcctt gatgaagcag ttggaaatag gcgtgctggc agttgacccc ttcaccgccg
     6481 aacacgatgc cgcccgtttt gcacacggcg ggactgccgt ctgaagcgcg gcttttgccc
     6541 aaactctcca tatcgagctg gttcagccac gccggcagca ggcgcaggtt gtggctgtac
     6601 ggaacggcgg tctgcccgtc cgcgtgttgg aaattgttat accacacggc aatcagtgcc
     6661 atcagaacgg gaatgttgcg gcgcggcggc gtgtggaaaa aatggctgtc catcgcgtgc
     6721 gcccccgcca acaactcgcg gaaacgcgcc ccgccgaccg caaccatcac gggcaaaccg
     6781 acgggcgacc agacggaata gcgtccgccc acccagtcgt acatcgcaaa cacgcgttcc
     6841 gccgcgatac caaaagccgc agctgccgca gtgtcggcag acaccgcgca aaaatggcac
     6901 gccgtttcgg attccgagaa ccccgcaccg cgataccacg ccttgactgc ctgtgcattg
     6961 agcagggttt ccggtgtttt gaaggacttg ctggcaacgc aaaacactgt cgtttcgggg
     7021 ttcagacggc ataaaaccgc atccaggcag gcaggatcgg cgttggcggc aaaatggacg
     7081 gcgatatgcc gtctgaacgg ctcaagtgcc tgcacgcaca ttgccggccc gaggtcggat
     7141 ccgcctatgc cgatgtggac aaaatccgta atccgttttc cggttgtccc ctgatacgaa
     7201 ccgtcgtcca aactgtgtgc aaacttcaac gcacgattta actcgcggcg gatttcgggc
     7261 aacacgtccc tgccgtccgc ataaacggca cccgcaccgt cgggcaggcg caaagccgta
     7321 tgcagcgcgg cacgcccctc actgttgttg accttcgcac ccgtccgcaa agcacgcatt
     7381 ttcccttcca aatccgccgc atcggcaaga ttgcagagca gttgcagcgt atcttcgccc
     7441 aaacggtttt tgctgtaatc gaacaacatc ccgtccaaac gctcgtgcat acgctcaaaa
     7501 cggtcgggtt cgcaggcaaa gcggtcgcgc aaaaggacat gacgcgtatc ctgataatgg
     7561 cgttcgagcg catgccatgc acgggtaaaa gcattcatct gttttccttg atttttcaga
     7621 accggattaa aatgtagcag aatgtagttt aacaaacggc agcggctttg gcgaatctcc
     7681 ggaacaccgc ccgccgcgtc caatagcacc ttatttccat atggcgaaaa tatagtgaat
     7741 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtatg gaaccgattc
     7801 acttggtgct tcagcacctt agagaatcgt tctctcttga gctaaggcga gccaacgccg
     7861 taccggtttt tgttaattca ctataccctc cggccggcat ttgacccgca acaatatcct
     7921 gcaagattta ttgtgtacgc ataaatgccg gacagccgcc taaataaggt ataatcgcat
     7981 ccgattctgt cccgtttgcc gcccgcaggc gggatgtcgg cggttttcat cttaccggga
     8041 acacgccggt actgtttttt aacgatgttt cttacatttt attccaatta ctttacgggg
     8101 ctagaatatg gctaaaaacg gaggattttc tttgttcgca aagaaagaaa aacgctttat
     8161 ctttgaaggc aggcattccg cctccgacaa actggtcaac ggcgaagtat ccgcgtttac
     8221 cgaagaagag gcgcgcaaaa aactggcaaa acgcggcatc cgcccgttgc agattacccg
     8281 tgtgaaaaca agctccaagc gcaaaatcac acaagaagac atcaccgttt tcacccgcca
     8341 gctgtccacg atgattaaag cgggcctgcc gctgatgcag gcatttgaaa tcgtggcgcg
     8401 cggacacggc aacccgtcta tgacggaaat gctgatggaa atccgaggcg aagtggaaca
     8461 gggcagctcg ttgagccgcg cattctcaaa ccacccgaaa tatttcgacc gcttctactg
     8521 caacctggtt gcggcgggcg aaacgggcgg cgtattggaa agcctgctgg acaaattggc
     8581 aatttacaaa gaaaaaaccc aggccatccg caaaaaggta aaaaccgcgc tgacctatcc
     8641 ggtatccgtc atcgccgtcg ccatcggttt ggtattcgtg atgatgattt tcgtactgcc
     8701 cgcctttaaa gaagtttacg ccaatatggg cgcggagctt cccgcactga cccaaacagt
     8761 gatggatatg tccgactttt tcgtctcata cggctggatg gtgctgatcg cactgggctt
     8821 tgccatatac ggcttcctta aattgaaggc gcgttcgatt aaaatccaac ggcgtatgga
     8881 tgccatactg ctgcgtatgc cgattttcgg agacattgtc cgcaaaggga cgattgcccg
     8941 ctggggcagg acgacggcga cgctgattgc ggcaggcgtg cctttggtcg atgtattgga
     9001 ctccactgcc ggcgcggcgg gcaatttaat ctatgaagaa gccacccggg aaatccgtac
     9061 gcgcgtcatc caaggtttgt ctatgacttc ggggatgcgt gcgacggaac tgttccccaa
     9121 tatgatgttg cagatgtctt ccatcggcga ggaatcgggt tctttggacg atatgctcaa
     9181 caaagccgcc gaattttacg aagacgaggt ggacaatgcg gtcggcaggc tgtccgctat
     9241 gatggagccg atcattatcg tgattttagg cttggtcatc ggcacgcttc tggtcgccat
     9301 gtatctgccg ctgtttaatt tgggcaacgt ggtcgcctga tttgccgcac agatccggcg
     9361 cggattcgct ctgcgccggt ttgtttttgt tttgaataca ttgagaacaa aatatgtctg
     9421 atttgtctgt attgtcgccg tttgccgtgc ctttggcagc ggtgttcggg ctgctggtcg
     9481 gaagtttctt aaatgtcgtc atttaccgcg tgccggtcat gatggaacgc ggctggacgg
     9541 tatttgccaa agaatattta aacctgccgc tgaccgaaga ggaaagccgt accttcaacc
     9601 tgatgaagcc ggattcctgc tgtcccaaat gccgcgtgcc gatacgcgcg tggcagaaca
     9661 tcccgattgt cagctacctg ctcctgcgcg gcaaatgcgc ctcctgccaa accaaaatca
     9721 gcatacgcta tcccttaatc gaactgctga ccggcgtatt gttcgggctg gttgcctggc
     9781 aatacggctg gtcttggatt acgctgggcg gattggtact gaccgcgttt ctgatttccc
     9841 tgacctttat cgatgcggac acccaatacc tgcccgactc gatgacatta cccttgattt
     9901 ggctggggct gatatttaat ttggacggcg gcttcgtgcc tttgcagtct gccgttttag
     9961 gtgcggttgc cggctatggt tcgatatggc ttttatgcgc ggtatataaa ctgattaaag
    10021 gcgaaatcgg tatgggcaac ggagatttca aactgattgc cgcattgggc gcgtggctcg
    10081 gcatatccgc attgcccgtg ctgatttttg tttcctcgct gatcggtttg gtcgcggcaa
    10141 tcgttatgcg cgtcgccaag gggcagcatt ttgccttcgg ccccgcactg acagtttcgg
    10201 gctggataat ttttacggca aacgattccg tatggcgggc ggtcaactgg tggctgaccc
    10261 atccggtgct gtaagatgac ggtatgggtc ggactgaccg gcggaatcgg cagcggcaaa
    10321 tcggcagccg cgcaatgttt tgccgatttg ggcgtgccgc gcatcgatgc agacgcggcg
    10381 gcgcactcgc tgacggcttc agacggcatc gccctgccgg aaatcaggcg gctgttcggc
    10441 gacaccgttt tcgacacaca gggtttgttg cggcgcgaca tattgcgtaa agaagtcttt
    10501 gcctccccat cgcgaaaagc cttgctcgaa tccatgatgt tgccgctgat tttctcagaa
    10561 atcaaaaaac agcaagaaac ctttaccgat gcggcttacg gcattgtcga aattccgctg
    10621 ctgacggaaa agcgtcaatt tatcatcctg atacggcgtg tcctgaccat aagtgcccct
    10681 gtggaaaaac gtatcggcag ggtgatggcc cgcagcgggc tgacgcgcgg cgaggtggct
    10741 gccgtcatca gccatcaggc atccgaatcc gaacgcctgc tgcttgcaga cgatgtgctg
    10801 ctcaatgacg gcagcctcaa aagcctgcgt gagaaaacaa tgcgcctgca cgcgttttat
    10861 tcagggattt tcgcctcaaa accaacacaa ggaaaacaca atggctgaat cgcggcaaac
    10921 acgccttcaa gtcaaatgtc cgacctgtca aacagcagta gtatgggaac ccgaaaacgc
    10981 attccgcccc ttctgttcgc aacgctgcaa actgatcgac ttgggcggat gggcagacgg
    11041 gaaatatacg gtttccggcc aaacggaaag tttgccggaa atatccgaac ccgacatggc
    11101 ataccgctga ccgccccgcc ttcccggcaa acaccctgaa agtcaaatgc cgtctgaaac
    11161 aaacacgctt cagacggcat tttcattctc aaacctaatc gttggtattt gccgttacct
    11221 cttccaatga agtaatgccc tgcataactt tcaaaatacc ggcccggcgc aaatccacca
    11281 taccctcctt ataggcaacg tccaaaatat ccacttccgt accgttgttc ataatcacac
    11341 gctgcatttc ttcgctgatg ggcataacct catacacgcc cgcacgcccc ttataaccct
    11401 gcccccggca acggtcgcaa ccgacggcgc ggtaaagttt ccaatctttt gcaaggtcct
    11461 catcggtgaa gccgacttcc ttcaaagcag aggcagacgg gcgttccact tcctgtttgc
    11521 agctcgaaca cagcctgcgt aaaagacgct gcgccataat caggctgacc gaactggcaa
    11581 tattaaacgg cgcgacaccc atattcagca tacgcgacaa cgtcgccggc gcattattgg
    11641 tgtgcagggt ggaaaacacc atatgccctg tttgtgccgc cttaatcgca atatcggcag
    11701 tttccaaatc acgaatctca ccgaccataa tgatgtccgg gtcctgacgc aggaaagact
    11761 tcaaagcagc ggcaaaagtc aggccctgct tatcattgac gttaacctga ttgatgcccg
    11821 gcaggttaat ctcggcaggg tcttccgccg ttgcaatatt taccgactcc gtattcaaaa
    11881 tattcaaaca ggtatagagc gacaccgtct tacccgaacc cgtcggaccg gttaccagca
    11941 ccatcccgta gggacggtga atcgcttcca acaacaattt tttctgaaac ggctcaaaac
    12001 cgagctggtc aatgttcaaa gacgcggcat cggaattcaa aatccgcatc acgacctttt
    12061 cgccaaacag cgtcggcaat gtgctgacac ggaaatcgac aggcttgccg cccttttgaa
    12121 aggtcagctg catcctgccg tcctgcggta tccgtttttc ggaaatgtcc aaacgcgaca
    12181 ttaccttaat ccgggaagca agctgccccc ttaccgcaat gggcggctga accacctcgc
    12241 ggagctgccc gtccacacgg aaacggatac gggcattgtg ttcgtaaaac tcgaaatgga
    12301 tgtccgatgc cccgctgcgc aaggcatccg acaaagtctt atggataaac ctcggaacag
    12361 ggccgtcttc tgcctcctcg ttgtcgatat acagggtgtg gctttcctct tcctcctgcc
    12421 cctccccaag ctcctgaagc agcgatgtcg aacgcgaacc cacccaatcg agcaaaccca
    12481 ccaactggtc atcctcgaca atgaccaact caacctcaat ccctgcggca gaaacggttt
    12541 tctgaatttg cggcatctgt gtcggatcgg aaaccgcaaa aaatactttg tcgccccgac
    12601 ggaaaaccgg cacacagtgg aactccacca tctgctcctc cgtcaacacc cccatcagca
    12661 ccctgtggcg cggataatga cgcaaatcaa gaatcgaata actgaacacc cttgcaatca
    12721 atgccgcaag cgacttgggc gaaatgacgc cgtctgaaaa cagcatcggc aacacttcct
    12781 tacccgcctg cgactccttg tagtaatgct cggcctgctc aacagtaacc acctggtttt
    12841 gaaccagaat cctcagcaaa cctacgctca tacgacctta tccccaaatt tattcattgt
    12901 tatacctgta cag
//
LOCUS       DAWKQJ010000045        12243 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000045 DAWKQJ010000000
VERSION     DAWKQJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.045"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(244..1536)
                     /locus_tag="WJL20_000952"
     CDS             complement(244..1536)
                     /locus_tag="WJL20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7683120.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1613..2581
                     /locus_tag="WJL20_000953"
     CDS             1613..2581
                     /locus_tag="WJL20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683121.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2782..4248)
                     /locus_tag="WJL20_000954"
     CDS             complement(2782..4248)
                     /locus_tag="WJL20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7683122.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4490..6607)
                     /locus_tag="WJL20_000955"
                     /pseudo
     CDS             complement(4490..6607)
                     /locus_tag="WJL20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(8089..8334)
                     /locus_tag="WJL20_000956"
     CDS             complement(8089..8334)
                     /locus_tag="WJL20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683123.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            8446..8874
                     /locus_tag="WJL20_000957"
     CDS             8446..8874
                     /locus_tag="WJL20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ7683124.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            8886..9362
                     /locus_tag="WJL20_000958"
     CDS             8886..9362
                     /locus_tag="WJL20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ7683125.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(9712..10200)
                     /locus_tag="WJL20_000959"
     CDS             complement(9712..10200)
                     /locus_tag="WJL20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ7683126.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(10268..11323)
                     /gene="aroG"
                     /locus_tag="WJL20_000960"
     CDS             complement(10268..11323)
                     /gene="aroG"
                     /locus_tag="WJL20_000960"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ7683127.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            complement(11485..11712)
                     /locus_tag="WJL20_000961"
     CDS             complement(11485..11712)
                     /locus_tag="WJL20_000961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683128.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            11939..>12243
                     /locus_tag="WJL20_000962"
     CDS             11939..>12243
                     /locus_tag="WJL20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683129.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         2807 a         3357 c         3025 g         3054 t
ORIGIN      
        1 acttagtttt attttaatcg actgtatggt ggattaacaa aaatcaggac aaagcgacga
       61 agccgcagac agtacaaata gtacgaaacc gattcacttg gtgcttcagc atcttagaga
      121 atcgttctct ttgagctaag gcgaggcaac gacgtactgg tttttgttaa tccactataa
      181 atgccgtctg aaaacctgtt tgcaggtttt cagacggcat tgttttcgtc gccggttcaa
      241 acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc
      301 cgccttctgt ttctgcgcgg caaacgccgc cttgtccgcc tgcgtcaatt cgggtgtcgg
      361 caatgcgact gaaacaggat taacgggctg cccgttgatg cgcgcctcgt aatgcaggtg
      421 cggcccggtc gaacgcccgg tcgaaccgac aaaaccgatg acctcgccgc cgcgcacatt
      481 gccttgtgcc tgcgaaaacg cgctcaagtg cgcgtacagc gtttccacgc cgttggcgtg
      541 gcgtatcatc accgcgttgc cgtatccgcc cttccggcct ttaaaggtaa tcacgccgtc
      601 ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg
      661 ccatgtgtgc aggatggggt gcatacggta gccgaacggc gaagaaatgc gcgtatagac
      721 cagcggctcg atgttgaagc cgcctttttc ctgcaacacc ctgccgtctt catcataata
      781 attgccgccc cctccgcctt ccttgtccga acggtaatag aatgcctgat gccttgtgcc
      841 gcccttaacg acttcagccg ccaaaatatc gcccgccgcc acctgctgcc cgtggaaata
      901 caggctgtcg tacatcaggc gcacggcatc gccttccttc aaaccgtcaa ggctgaagcg
      961 gccggcgaaa atcccgctta aggattcgcg gatttcgacg ggcacttccg cccgcgccag
     1021 cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagc gtcggcaaaa ccttcatatc
     1081 cgcctcagaa gccgaccgcc gccatatgcc gcctttcttt tccaaagcga ccagattgcg
     1141 ctcgccgtct tcgtcggtaa aaaactgtac ttcgcgcgcg ccgccgtcgc cgccgaccaa
     1201 aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc
     1261 cgtgattcgg gcaatctcgt cccgcgccat acccgaacgc gccagcacgt ccgccagcga
     1321 gtcgcccggc tgcaccgcct cctgcaccca atatgccgtc tgaacgccgc tgccgcccca
     1381 agacagcggc ggcagatttt gttccacgcg ctgcggtctg acgcgttccg tcctctctgt
     1441 caaagcaatg tatgccgccg acaccaaaat aatcgaaacg gcaagcgcgc gcagcgcgta
     1501 tttccgatgt tttgccgaaa gtgggaagac agccatattg ttcctttttg acggcgtttg
     1561 cgccgaatcc tctataatat ccgcccgatc cgcatttgtc agacatcaaa cgatgaaact
     1621 caccctcgcc ctcccctcgc tcaatttgga tgaagacgaa atccgaatac ctctatgcct
     1681 gcccgccttc aacaaaatcc tgcaatacgg ctcgctgcac cgccaaagct gcacggcttc
     1741 ggcattttac gcgcgttatt tgtggtgcgg cagactggcg gaacgcgcgg cgcaaagcct
     1801 gaatatgccg tctgaaaccg tcgcgctcgc cacgcccgtc tggcaaaaga tggggatgca
     1861 tcaggcaaat gtgctgacgg cggaatattt ggatgtcggt acggatgaag cggaacggct
     1921 ctgccgcgat ctgtcggcgt tttacggcga tattccgtgg cgtttcgttc ccgtcctgcc
     1981 cgaattatgg ctggtttccc ttccccgcgc gtaccgctgg ggcgcgaagc ccgttttgga
     2041 cttgggcgga ttgttgggcg cggacgatca gccggacgga gaagatgcgt tggaatggct
     2101 gcgcgtccaa accgaaatcc aaatgtggct ggcggcgcac cccgtcaacc ataaccgcaa
     2161 gaaacgcggg ctgcccgagt tgaacggctt atggctttgg ggcggcacaa acggcggcac
     2221 gcaaggcggc acgcttttcg ccgatacggt ttggagccgt ttccaccccg accgccgcgc
     2281 attgcccgac aacttccgcg cctatgaaga aacggcggca cacctgcccg acacgcacca
     2341 tatcctgttt atggacgatt tgcgcctgac cgccctgaca ggggacaggg agcggtatgc
     2401 ggcaatattg cagcagtggg aagaacgctg gttcgcaccg ctttacgaag ccgtccgcat
     2461 cggcaaaatc aaacggctgg acatcgccac ggacggacaa cacggcggta cgctgacctt
     2521 caaacccgcc gacaggtgga aattttggcg cggcgcgaaa acctttgacg gaatctggtg
     2581 aacccgcccg ccgcaaccgc gacaatgccg tctgaagccc gaatcgggtt tcagacggca
     2641 ttttacctcg catcgccgtt atgccggaaa aaccggaatg cgtttccgca cacgcggcgg
     2701 cacgcaaagc accgcccgaa agccgccgat gcccgccgtg ataccgccgc cggcagggtt
     2761 tcctccgaaa aggaacagca atcagaacgt tttattaaac tcgacaaacg cccgattttt
     2821 ctcgtattcg ttgaacacat cgttactccg cgtttcgcgg tgcgacaacg tcaggcgcgg
     2881 cgtgatgcct ttgaaatgca atgcccggtg ccaaaggctc aaggatgtgt tcaattcttt
     2941 atccctgcgc ctttcccctt taaaaccgct gaaaaagccg ggtttttcat aatgccgttt
     3001 cgccgcgccg aggcgcaaca gcgaagacag gccgctgccg ccccattcct gcccccaggc
     3061 aaagcgcagg ccgtaacggt tgaaattgtc gccccggtcg gcggggttgc gctcgcggta
     3121 aaaatccaaa ccgcccatcc aatattggcg cgcattccgg taaaacacca gcgaattgga
     3181 aatttgcaaa tgggtattgt cggaacgcgc ccggcgcgta ttcttcaaac gcccccactc
     3241 cgccgaagac aacgtttgcc atttcggggt ttgccaacgg ttgaaataaa ggcgtgcgcc
     3301 gttggtgtaa gaataagcgt cgttgccgta ggtgcggcgt tcgtggaaca ctgccagccc
     3361 ggcatctttg cgccggtcgg caaaaccgat gccgccggaa acgcctgccg tcatatcgtt
     3421 gaatttctta ttccccggat aaaccctgcc ggacacgtcg ccgcccgccg tcgtgtacca
     3481 gccgtttttc agcgaccatt ttttctccgc gccgagccgg taattgaccg ccgtgccgtc
     3541 cacctgtttc gggaaagtcc atttgccgta ctgctgccgt ttcggggctt ggttgatatt
     3601 gtgttcgcgg gtgacgctga agccgccatt taccttccac gcatcgcgtt cgcgcaatgc
     3661 cttgcggtac agctcgacct gctccatcag ctgcggcggc aggttttccg ccttcaggcg
     3721 gtcgaactgg tctgccgccg cctcgttctg cctgttttca aacaatgccg ccgccaaacg
     3781 catacggacg gcgggcgcgt cgggttgggc ggcaatcaat tcccggtaat gggaaatcgc
     3841 ctccttcacc ctaccgtctg cctgcgccaa aatcccttgt gcataaagtg ccaacatctt
     3901 atcctgctgc gcctgttgta ggtaaatcgg caaaataacg cggataccgg caatattgtt
     3961 tgagaccact gcggaataca tcgcgcggga caacaattcg ggatttttca gcagggtttc
     4021 tccgtcaatc tgcaaaactt ttcctttttc ccgcacctgc cccggtacct tctccctgtc
     4081 gattggtttg acctccgctt catgaagcct gaactcggga cggcttctca aatccggttc
     4141 gcgcggtgtt tcttcggcat atgccgcgct tgccaataaa ggcagcagca gcatccatct
     4201 gtttcgtgca ggcataaatg tcttcccaca aaaataaaaa ataaccatta tcaatcggcg
     4261 gattgtatca gttttttatg ccgctgttcc aaaccctgcc gcccgatgcc gcgacaatgc
     4321 cgtctgaagc cagaatcggg tttcaaacgg cggcaaaaaa ccggcggttt tccgccggtt
     4381 tcatcattat gccccgccct tcggggcggc agggttcgga ttggtttgcg gggcggcgcg
     4441 cggtgccgtg ccggtgtgcc gcctgtacgt caaaacattt cccgacgcat caaaacagcc
     4501 atttgagcga caatgcggct tctttgtcgc cgtccgtcct tttgccgtat ccaatgcgtg
     4561 cggacatatg gcctttccaa ccggcttcaa tgccgaaccg cccttcgagt gccgtcctgc
     4621 ctgccagcgt ctgtttttcg ccgtccattt ccacgccgaa agattttgac ctgtgcaaaa
     4681 cattaaaagc gacaaaaggc tgaagattga caccgttacg caaagcaaaa cgggtttttg
     4741 cccgaatgcc ggcgcggctt tgccactgac cgctgccgag cagtccgacc gccgtcccct
     4801 cgctgtcggt aaagccgccg tttacgccca agtaggtaaa ctgcgcctgc ggttgcaggt
     4861 aaaaccgcac attattgcct tttccgacaa cgccttccgc cacaagcgcg ttgtagccgc
     4921 cttcgacaga agccgtccaa cctttggttt tgtagcgttc cgcacggttt tcatcattga
     4981 tgcggtgttt gaaacgttgg tattgcaacc agccgtccaa atacgcaccc gtttgtttat
     5041 cgcgcaactg atgccacaca gcataaacac ccccgccata accatgcaaa taactgcctg
     5101 ccgcaccgcc tttgccgttg actgatgcgt gctggccgac cctgccgccc atcacgccga
     5161 ttgccagtcg gctgccttca ttttgctgta caaacacctc gccgccgatt tgcacgcctt
     5221 tgcgccgccc gtccgcagcc gcgccgcccc gtatattttg atgcgaccgg ccgccgatga
     5281 agcgcagcca caatttttga cgggacggat cggcggcata cacgtcgtcg cccctgtcgg
     5341 cggcacgcag cccaaacaaa gtatttgccg cataagcctg ttgggcatat aaaaccgctt
     5401 ccgcctcggg gatagccaaa cgctgcacgc ccaagcaaaa tccgccttcg cattgcttga
     5461 gcaataattc gtacagtccc tgccggtaat tttgtttaaa cgcaaacgaa ttagagtctg
     5521 ccttttccgc cgcaatcagt ttgcgcccgt cgagcgtttt cagattttcc ggccggcgtt
     5581 ccaaaaccac gccggattgt ccgctgactt tttcttcaaa ggtcagggcg ggtttgtccg
     5641 actcccgcac gtcgtagccg aacaggaaat ccgcgccgcc cgcataatcc ttatggacgg
     5701 tcagtttttc atctttcgtg ttcaggcgga tgcgggcatt atcggaagtt tccagcgtat
     5761 ggatgtcgga atggcggcgc ggttcccaaa gggaatcttt cagcgtcatc ttggtcagtt
     5821 ccaacacctg tccagcgacg tgggatttgt tttcgatttt gacgtgtaat tttttattga
     5881 agatgttgtc gagattggtc ggtgttgtat tactatgagg agtgagtacg gaatcttcta
     5941 ccaatttatt aattaaatcg gggtttttct gtttgagtat actaaatttt ggtccaagct
     6001 gttctatata cgcctcctca gtccgttttt tattttcttc ccaagcttcg ggtcttgttt
     6061 tgtataaatc ctgtaattgc ttcttaaccg catcctttaa attatccccg taagtatctg
     6121 taatatattt cttaacaacg ggcatattat cgacggcgag tatgccgcta ttgtagtttt
     6181 taccttggat attgatttcg tcatacccat attcggatga attttgatcg gttttaccgc
     6241 tggaaaaata atgggaagtg ccgggtttcg gcttgtctga aaatgaaaaa tcggcatcgt
     6301 cgccgatttt ggtagaacga gtatttgaac caagctgagc tctgaacata ccgaagcaat
     6361 caaaagatat gctgcacggg ccgcgccaaa cgtgggaatt tctgtcttcc gccgcttcta
     6421 aaataaattt gtttttaatc tcggcttctt tgacgggatc gtacgcatcc gaacctcaaa
     6481 catatggcgt gcaaaacagc gataaaacag ataatgttaa tagattaacc cgaccgcttg
     6541 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg
     6601 cttgctttca gtgtcatgag aaattttcct ttgtcaagtg ttaaaattat aatgattata
     6661 tactacatta tactacatcg caatgaaaaa tcaggaaaag caaaaaaccc tccgccgtca
     6721 ttcccgcgaa agcgggaatc tagaatttta atgccgcaag aatttatcaa ggatggtcgg
     6781 ggtttaaagg tctggattcc cgcctgcgcg ggaatgacgg tttagaaatc acccgaaaca
     6841 agaaaaagaa ccgaaaccga acaagccgga ttaccgcctg cgcgggaatg acgggatttc
     6901 aggttactgt ttttgatttt ctgttttgtg gaaataacgg gattttggat gatgtgcatt
     6961 tatcgagaaa actaaaaacc ctccgccgtc attcccgcga aagcgggaat ctagaccttt
     7021 cggtgcggaa acttatcgga taaaacggtt ttccctgcat cctggattcc cactttcgtg
     7081 cgaatgacgg gatataggtt tcactacgga cgtgttcaga ttaccgcctg cgcgggaagg
     7141 acgggatttc aggttgctgt ttttgatttt ctgtttttgt gggaataacg ggactttgga
     7201 tgatgtgcat ttatcgggaa aacaaaaaac cctccgccgt cattcccgcg aaagcgggaa
     7261 tctagaattt taatgcggca agaatttatc ggaaaaaact gaagtttaaa gacctagatt
     7321 cccgcctgcg cgggaatgac ggtttagaaa tcacctgaaa cgagaaaaag aaccgaaacc
     7381 ggacaagtcg gattcccgca cgggcgggaa ggacggaatt tcagattgtt gtttttgatt
     7441 ttctgttttg tgggaataac gggattttgg atgatgtgca tttatcggga aaactaaaaa
     7501 ccctccgccg tcattcccgt gaaagcggga atctagaatt tcaatgccgc aagaatttat
     7561 cggggatgtc cggggtttaa aggtctggat tcccgcctgc acgggaatga cggtttagaa
     7621 atcacctgaa acgagaaaaa gaaccgaaac cagataagtc ggattcccgc acgggaggga
     7681 aggacgggat ttcaggttac tgtttttgat tttctgtttt gtgggaataa cgggattttg
     7741 gatgatgtgc attttcggga aaactaaaaa ccctccgccg tcattcccgc gaaagcggga
     7801 atctagaatt ttaatgcggc aaaaatttat cggaaaaaac cgaagtttaa agacctagat
     7861 tcccgcctgc gcgggaatga cggtttagaa atcacccgaa acgagaaaaa gaaccgaaat
     7921 cgaacaagcc ggattaccgc ctgcgcggga atgacggctc aaaagttacg aaacgaaaaa
     7981 taaccgaaac cagacaagtc ggattcccgc gcgggcggga atgacgataa gcgtaatttc
     8041 aatctgccat attgtctttt cacttgcctg aaaacagatt tcagccacct atccctgcat
     8101 ccggcggctg atgtagtcgg caatcaaatc ccgcttttcc caaaaaaact tctgcatttt
     8161 ttgttcggaa tacagtttgc ggaccagagt gggcaaaccg ctgtggacga tggcggcgat
     8221 ttctttgttg cctttttcct cccgcatcgc cgtctgaaaa tccaaacccc tgcctttcag
     8281 atatgctgca aggcggttgc ggatgtggga attgaattcg atggatttga gcattttgcc
     8341 tccgttttgc ggtaaggatt tattcgattt gtttatccgc ctgattttat cacgtttccc
     8401 aagcggtttg cctgtacaat accgcttttc ggcagggtgt gggcgatgat gttttcttgg
     8461 ttcaagctgt ttcacttgtt ttttgtcatt tcgtggtttg cagggctgtt ttacctgccg
     8521 aggattttcg tcaatatggc gatgattgat gtgccgcgcg gcaatcccga gtatgtgcgt
     8581 ctgtcgggca tggcggtgcg gctgtaccgt tttatgtcgc cgttgggctt cggcgcggtc
     8641 gtgttcggcg cggcgatacc gtttgccgcc ggctggtggg gcagcggctg ggtacacgtc
     8701 aaactgtgtt tgggcttgat gctcttggct taccagttgt attgcggcgt gctgctgcgc
     8761 cgttttcagg attacagcaa tgctttttca caccgctggt accgcgtgtt caacgaaatc
     8821 cccgtgctgc tgatggttgc cgcgctgtat ctggtcgtgt tcaaaccgtt ttgacggagc
     8881 atcttatgcc gatagaaatc gaacgccgtt ttttgattgg aaacgacaac tggcggcaat
     8941 acgccgacga accgctgctg ttgaggcagg gttatctgtc tgttgaaaaa gaacgcaccg
     9001 tccgtgtccg cattgccgga caacgggcgt ggctgacgct gaaaggctat atttcggaaa
     9061 tcagccgcag tgagtttgaa tacgaaatcc cgcttgccga tgcggaaaag atgatggaaa
     9121 cgatgtgccc gtttaaaatg gaaaaatggc gttatccggt cagatggggc ggcagcctgt
     9181 ttgaaatcga tgtttttctt ggcgataatg cgcctttggt cgtcgcggaa atcgagttgc
     9241 ccgacgagaa tgccgatttt gaccgtccgg actggctggg acgggaaatc actgccgacg
     9301 gtatgtttac caatgcttat ttgagcaaac atccgttctc aagctggaaa aatgccgtct
     9361 gaagcggctt cagacggcat tctccgacaa ccatcgcaaa tccaaagaca gcctctatat
     9421 ccttatttct gctttctttg ttccattatt cccaataaat ccccataacc taaaatctcg
     9481 tcattcccgc tcaggcggga atccggttct ttaaacttcg gttttttccg ataaattgct
     9541 ttagcttttc gtttccggat tcccgctttt gcgggaatga cgatggaaga ttgttgttat
     9601 ttcggataaa ttaccctaac tttaaaatcc cggattccca tcttcggggg aataacaata
     9661 ttttctattt cagcccatca tttgcggggg attttataga gagtttatat ttcaatatct
     9721 caaataatgc acaaaagcat agcgcgtgcc tttgctgctg acacggcgtt ccgtccgctc
     9781 tgcttctttc caatgcgtcc ggtctattgc ggggaaaaat gcatctcctt ccacagacaa
     9841 atccacttcg gttatccgca aatcggtcgc caatggcatc gcttgtccgt atatctgcgc
     9901 gccgcccata atgacggctt cttccgcgcc tgcgcacaat gccaatgccg cctccaaact
     9961 tgccgccgtt tccgcgcctg ccgcgcaata atccgcctgc cggctgatga cgatgttcct
    10021 ccgtccaggc aggggtttga cgggcaggga ttcccacgtt ttccgcccca taatgacggg
    10081 tttgcccaag gtataggctt tgaaaaatgc gaaatcttcg gggatgtgcc aaggcatagc
    10141 attgcccgcc ccgatgcaca ggttttccgc acacgccgca attagggtta ttttgagcat
    10201 atttcctcca caaaaatgcc gtctgaacgc ttcagacggc attttatgac attctgcgtc
    10261 aaaaatctca accggcgcgc gccatacgtt ttttgtttgc acctgccaac aatgccaaca
    10321 gttcttcagt cgcgtcccaa ccaatacacg catcggtaat gcttttgccg tacacttccg
    10381 gcttgtcctg cctgccttcg accaaatggc tttccaccat cacgcccata atattgccgc
    10441 cgtcctgttc caattgggcg gcaatgtctt gcgccacttc catctgccgg gtgtaatcct
    10501 tgcggctgtt ggcgtggctg caatctatca tcagcttatc ggttacccct gccgcacgca
    10561 gttgttccgc cgcctcgctg acgtgtcccg catcataatt cggctctttt ccgccgcgca
    10621 aaatgacatg acagtcggga ttgccgccgg tatggacaat ggcggaatgt ccggccttgg
    10681 ttacagacaa gaaatgatgc gaatggctcg ccgcaccgat tgcgtcgatg gcaatcttca
    10741 aattgccgtc cgtaccgttt ttaaagccga cggggcagga cagcccgctt gccaattcgc
    10801 ggtgaacttg gctttcggtc gtccgcgcac cgattgcccc ccaagaaatc aagtccgcat
    10861 aatattgcgg cgtaatcata tccaaaaact cggtagaggc aggcataccc atattgttca
    10921 gcgacaacaa caggctgcgc gcctgacgca aaccgaaatt gatgtcaaac gtaccgtcca
    10981 aatgcgggtc gttaatcaaa cctttccaac ccaccgtcgt cctcggcttc tcgaaataaa
    11041 cgcgcatcac aatcaaaagc tcgttttcat actgcttgcg gagtttcaac aaacgctccg
    11101 catattccaa cgccgctttc ggatcgtgaa tcgaacacgg cccgataata accaacagcc
    11161 gcttgtccct gccgtgaacc aaatcggaaa tttcctgacg ggtgcggtga accaagcccg
    11221 aagcctcttt ggaaatcggc agctcgtaaa gatgggcaat cggcggcaac aactctttaa
    11281 cttctttaat cttaatatcg tctgtggggt aatggtgtgt catcttgcat ctttcctgat
    11341 ggttttttat acgcggctta ggttacatcg tttaccttgc acaaacaagt gcaacatatt
    11401 actacaaagc tattttattt caaaataact gatatatctt ttttcataga ttttaaattt
    11461 catattttta atattttagg aattttattc caaagaaaaa gaaacagatt gataaaaaca
    11521 atacccgcca ttcattcgga caaaaccgca cggaacatcc gttttcccat ttccacgaca
    11581 atccggtaca gaacaccccg ctgccgattc cgcctgcaca tccggcaaaa tttcatacgc
    11641 aaggaaaatg ttgaaaacaa aaagaactaa ttgcttgaaa aaagatttaa ttggaatgcg
    11701 ggtatttccc acactttctc catttattca tcctcattat aggaagaaaa acatgaaaaa
    11761 accctgcttg cactctcact tgcagccgtg tccgccgtta cggtagccga tattcctgag
    11821 tctaacggca agggcaaagc catcatcggc gataaggaaa aacaccgtta ccaagcatga
    11881 aaaccttgtt ttgaaagtct atggcgaaat ccgccaagat ttgggggacg gatgatccct
    11941 gaccccttgg gcggatgcat atgcagattt gcgcggcaaa accaaagtga tgacgaccca
    12001 gatgtgtgct tcccgcgatg tcagcaaaag cgccaaaggt tggagtgtcg gtatcggtct
    12061 gaatgtaggc aaacagttga ccgacagcgt cggtctcgag tttgatccat actaccgtca
    12121 caaaacaatc tgcaaacccc gtgagattgt tttggacggc gacaaaacca aaatgggccg
    12181 ctccaaatcc aacgagtacg gcttccgcgt aaccgcaacg ttctatagtc aattaaaatc
    12241 aaa
//
LOCUS       DAWKQJ010000046        12139 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000046 DAWKQJ010000000
VERSION     DAWKQJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.046"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            207..944
                     /locus_tag="WJL20_000963"
     CDS             207..944
                     /locus_tag="WJL20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683130.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            947..1633
                     /locus_tag="WJL20_000964"
     CDS             947..1633
                     /locus_tag="WJL20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7683131.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            1793..2656
                     /locus_tag="WJL20_000965"
     CDS             1793..2656
                     /locus_tag="WJL20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683132.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2718..3287)
                     /locus_tag="WJL20_000966"
     CDS             complement(2718..3287)
                     /locus_tag="WJL20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ7683133.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3445..4305
                     /locus_tag="WJL20_000967"
     CDS             3445..4305
                     /locus_tag="WJL20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ7683134.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTLETFDHI
                     LKQLGIDYRP"
     gene            4440..4778
                     /locus_tag="WJL20_000968"
     CDS             4440..4778
                     /locus_tag="WJL20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683135.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5146..5499
                     /locus_tag="WJL20_000969"
     CDS             5146..5499
                     /locus_tag="WJL20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ7683136.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5489..6355
                     /gene="atpB"
                     /locus_tag="WJL20_000970"
     CDS             5489..6355
                     /gene="atpB"
                     /locus_tag="WJL20_000970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ7683137.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGIAGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6412..6648
                     /gene="atpE"
                     /locus_tag="WJL20_000971"
     CDS             6412..6648
                     /gene="atpE"
                     /locus_tag="WJL20_000971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ7683138.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6719..7189
                     /locus_tag="WJL20_000972"
     CDS             6719..7189
                     /locus_tag="WJL20_000972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ7683139.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7194..7727
                     /locus_tag="WJL20_000973"
     CDS             7194..7727
                     /locus_tag="WJL20_000973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ7683140.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7738..9285
                     /locus_tag="WJL20_000974"
     CDS             7738..9285
                     /locus_tag="WJL20_000974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ7683141.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9310..10185
                     /gene="atpG"
                     /locus_tag="WJL20_000975"
     CDS             9310..10185
                     /gene="atpG"
                     /locus_tag="WJL20_000975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ7683142.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10223..11620
                     /gene="atpD"
                     /locus_tag="WJL20_000976"
     CDS             10223..11620
                     /gene="atpD"
                     /locus_tag="WJL20_000976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ7683143.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11631..12053
                     /locus_tag="WJL20_000977"
     CDS             11631..12053
                     /locus_tag="WJL20_000977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ7683144.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         2993 a         3103 c         3125 g         2918 t
ORIGIN      
        1 ttttgttaat ccactatatt gtccgaaatg gttgcccgct tcctgcctta ttgacaaaaa
       61 aacgctttcc cgataatatc ctacgaaaat taacctgccg atttgacaca gcttgcgggc
      121 ataacagcta aagcgttccg acaattccag ctttatcttc cgcgcccgtt gtgtccgaca
      181 tcgggctttg ttgtatggga aagacaatga ttattttgga caaggtttcc aagcattacc
      241 aaacgcgcga caagacccgt tttgccgctg tcgagccgac cagcctcgaa atccgcgacg
      301 gtgaaatctt cgggctgatg ggttattcgg gtgcaggcaa atccaccctg ttgcgcctga
      361 ttaacctgtt ggaacgtccc gacagcggca aggtcaacgt ctgcggacaa gagctgaccg
      421 cgctcgatgc cgccgcattg cgtcaggctc ggcagaatat cggcatggtg tttcagcagt
      481 tcaacctgtt gaacaaccgc accgtcgccg gcaacgtcgc cttcccattg gaaatcgccg
      541 gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgaatg tctcgaaatc gtcggactga
      601 ccgaacgcgc cggccactat cccgcccagc tttccggcgg gcagaaacaa cgtgtcggca
      661 tcgcccgcgc gctcgcatcc aagccgcaag tcatcctcgc cgacgaaccc acttccgccc
      721 tcgaccccgc caccacgcgc agcgtcttgg aatgtttgga agacatcaac aaacgcttca
      781 acgtaaccat cgtcatcgta acccacgaaa tgagcgtcat cagacgactg tgcgaccgcg
      841 ccgccctgtt ggataaaggc aaagtcgtcg aaatcgtcga agtacgcggc aaccaaatcc
      901 acgcccgatc cgacatcggg cgcgaactga ttcgggagga ctgaatatgg caggcttaac
      961 atttgaacaa accgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg
     1021 cgaaaccttc gtaatggtcg gtttgtccac cacattcgcc gtcgttttcg gcacgttgct
     1081 gggcgtgttg ctcttcgtaa cttccagccg ccaactgcat tacaacaagc cggtgaactt
     1141 cctgctcgac aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc
     1201 gatgataccc gccacacgcg ccatcgtcgg cagcaccatc ggcccggttg ccgcctcgct
     1261 ggtgttgagc gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga
     1321 agtccccaaa ggcgtgattg aagccgccgc cgcgatgggt gcgcctccga ttgccatcgt
     1381 ctgcaaagtc ctcttgaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc
     1441 catcgggctt ttgtcataca gcgcggcggc agggatgata ggcggcggcg gcttgggcga
     1501 cctcgccatc cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc
     1561 cctcctcgtg ctgctcgtta tcctgattca aagcaccggc aacacgttgg cgcggaaact
     1621 cgacaaacgt tgaacccaaa atgccgtctg aacgccaaaa cccccaccgc tatccgaaaa
     1681 atgctataaa attcccctgt tcgcggcaaa tgccgtctga acgccgaatc cggacggcag
     1741 gactccctgc ccgtcatttt tgtttgaaac cgccacaaca tcaggagaaa atatgaaaac
     1801 cttcttcaaa accctttccg ccgccgcact cgcgctcatc ctcgccgcct gcggcggtca
     1861 aaaagacagc gcgcccgccg catccgcttc tgccgccgcc gacaacggcg cggagaaaaa
     1921 agaaatcgtc ttcggcacga ccgtcggcga cttcggcgat atggtcaaag aacacatcca
     1981 acccgagctg gagaaaaaag gctacaccgt caaactggtc gagtttaccg actatgtgcg
     2041 cccgaatctg gcattggctg agggcgagtt ggacatcaac gtcttccaac acaaacccta
     2101 tcttgacgac ttcaaaaaag aacacaatct ggacatcacc gaagtcttcc aagtgccgac
     2161 cgcgcctttg ggactgtacc cgggcaagct gaaatcgctg gaagaagtca aagacggcag
     2221 caccgtatcc gcgcccaacg acccgtccaa cttcgcccgc gtcttggtga tgctcgacga
     2281 actgggttgg atcaaactca aagacggcat caatccgctg accgcatcca aagcggacat
     2341 tgccgaaaac ctgaaaaaca tcaaaatcgt cgagcttgaa gccgcgcaac tgccgcgtag
     2401 ccgcgccgac gtggattttg ccgtcgtcaa cggcaactac gccataagca gcggcatgaa
     2461 gctgaccgaa gccctgttcc aagaaccgag ctttgcctat gtcaactggt ctgccgtcaa
     2521 aaccgccgac aaagacagcc aatggcttaa agacgtaacc gaggcctata actccgacgc
     2581 gttcaaagcc tacgcgcaca aacgcttcga gggctacaaa tcccctgccg catggaatga
     2641 aggcgcagcc aaataaggca gtcgtataaa atgatgccgt ctgaaccgta tccgtgttca
     2701 gacggcattt ttgtccttta atccgccatt ccctgccatt ccgccgaatc cggcgtatcg
     2761 attccgaaca gcgacaaagc gtgtgcaaca ctgtgcgcca ctatgtcgtc cgccgtctgc
     2821 ggtttgcggt acatcgcagg aacaggggga aacaccacgc cgcccatttc cgttacccgc
     2881 ttcatattgt ccaaatgggc aaggttcagc ggcgtttcgc gcaccatcag caccagccgc
     2941 cgcctttcct tcaaaaccac atccgccgca cgcgtcaaga ggttgtcgcc gaagccgtgc
     3001 gcgaccgagg caagcgtccg catcgaacag ggggcgacca gcatcccgtc cgttttaaac
     3061 gtaccgctgg caatgcacgc cccgatattg ccgatcggat gcacgaagtc cgccaaggca
     3121 tatacctcat ctctcgcata agccgtttcc gaagcgcgcg ccatctccgc acctttcgat
     3181 accacaaggt gcgtttcgat atcttgcgcg cgtaaaagtt ccaaagcctt cacgccgtat
     3241 tggaaaccgc tcgccccact gatgccgatt atcaaacgcc gtaccatcat ccgcctttcc
     3301 cataaaaccg cctgcaacgg caaaccggct attatagtga aaaaacagaa atccgataaa
     3361 tacggataca aattgtcggc aacacccaat atccgataaa atatccgatt taacatccta
     3421 tctgaatagg cacgggaggg cggtatggca aaagtaaaag gcggattggg gcgcggtttg
     3481 gattcgctgc tcgccaacgg cgcagacaac agcagcggcg accgattgac cacggttgcg
     3541 gttaaagata tccggcccgg ccgctatcag gcgcgtgttc aaatcgatga cgaagccttg
     3601 caggagctgg cggattcgat taaagcacag ggagtgatac agcccgtcat cgtgcgcgaa
     3661 cacggactgt cccgatacga actgattgcg ggggagcggc gttggcgtgc cgcacagatt
     3721 gccggcctga ccgaaatccc cgccgttatc aaaaccatca gcgacgaaac cgcgttggca
     3781 atgggtttga tcgaaaacct ccagcgcgaa aacctcaacc ccatcgaaga agcacaaggc
     3841 ttgaaacgcc ttgccgacga gttcggactg acccacgaaa ccatcgccca agccgtcggt
     3901 aaaagccgaa gcgcgatttc caacagcctg cgccttttaa gcctgcccga acccgtgcag
     3961 gaaatgcttt accaacgccg cctcgaaatg gggcacgccc gcgcactgct gaccctgccc
     4021 gtcgtcgaac agctcgaatt ggcgcaaaag gccgtcaaaa acggctggtc ggtgcgcgaa
     4081 gtcgaacgcc gcagccaagc cgcccttcaa aacaaacgtc ccgagcccaa aaagactgcc
     4141 gccgccgaca tcggccgcct gaatgatttg ctgactgaaa aactgggtgt caacgccgaa
     4201 atcaaaaccg ccaaccataa aaaaggcaaa atcatcctgc atttcgacac gctcgaaacc
     4261 ttcgaccata tcttgaagca gttgggcata gattaccgac cttaattttg cgggatatac
     4321 cgtctgaaat atagagaata gctttccaga ttttaagtgg gaaatataat tctattgaca
     4381 tttttctgct tcacgtaaga atcgttttcc tgttttcatt tttaattttc gaggaaatta
     4441 tgaacacacg catcatcgtt tcggctgcgt tcgttgcgtt ggcattagca ggttgcggct
     4501 caatcaataa tgtaaccgtt tccgaccaga aacttcagga acgtgccgcg tttgccttgg
     4561 gcgtcagccc aaatgccgta aaaatcagca accgcaacaa tgaaggcata cgcatcaact
     4621 ttaccgcaac tgtgggtaag cgcgtgagcc aatgctatgt taccagtgta atcagcacaa
     4681 tcggcgttac cacttccgat gcaatttgtt tgggaggcgg aacgcacaaa ggcaaaagtc
     4741 aatgcaatgc tttgcttaaa gcggcaggcc gttgctaatc ctttattcgg aaaaggtcgt
     4801 ctgaaaatat tttcagacga ccttttattt attgagcaaa ttccccaaac tgccgacaat
     4861 ccgaccgata aactgctaca atttcgcgtt gattttttat tatttccacg taaagcaaac
     4921 aaatcacgta agaacattaa aaatgtaaag aaattgcgga caaactcctt aatatcctgt
     4981 aattatatgt aatgtgtgtt agaatgacac ctgtcgctgt tattcaggcg gcgagcgcgg
     5041 cggattggga tacgttgttt cacaaagatt aactatcgct tgacgtaata aggcttctaa
     5101 attatattag ccgcgcccca aatccgaggc ggaaacattc cctttatgaa gcagattatc
     5161 atcctacaat ccgccgtatt gtccatatgc gccgcagttg cctttgctgt atggggtttt
     5221 gccggattcc tttcggcggt cggtggcggt ttgtcctacc tgcttcccac gtttgttgca
     5281 gttttacttt taaaactttt caggggaaac cccttcctgc aaagcaggat gttcgtcttc
     5341 ggagagattt taaaagtagt gctgtcgctg ttgtccatgc tcgccgtatt tgctgtatgg
     5401 catcaatcgt tggtgttcgc cccgtttctg atggggctgc tcggtgtcag ccatttggtt
     5461 tttttagtat tgttgagagt gaaagattat ggcaggtgaa accattaccg ctgccgacta
     5521 catcaagcac cacttgcaga gcttgaccag tttgtcggat gttactcagg gtcagggact
     5581 gaaaaacatt gctgattttt cgtttattaa ccttgatgcc gtcttttttg ccgtcctgtt
     5641 gggcgtaatc ggcagcttcc tgttgtggcg cggtgcgaaa aaagcaacgg caggcgttcc
     5701 cggacgtttc caggctgccg tggaaatctt gtttgagttt gtggacgata tgtgtaagag
     5761 catcattcac aacgaaaagt ctagaaaagc cgtcgcgccg ttgggtctga cgctgtttgt
     5821 ctggattttt ctgatgaacg cgatggatat gctgccggtc gatttgctgc cgatggtatg
     5881 gcagggcatt gccggcaacc atcacgccct gctgcgcgtc gtaccgactg ccgatttgaa
     5941 caccactttg gcactcgctg tcggcgtgtt gctgatttgt atctattaca acatcaaaat
     6001 caaaggattg ggtggctggt ttcacgagtt gttcagcgcg ccgttcggtg caaaactcgc
     6061 acctgcgaac tttctgttga atttggtcga gtttctttct aaaaccgtat cccacggtat
     6121 gcggttgttc ggcaatatgt atgcgggcga attggtattc ttgctgatag ccttgcttgg
     6181 cggtgcttgg gcggcttccg gcagcgtcga agtcatggat ccgattctgt ttgtattcca
     6241 cattattgcc ggtttggcat gggcgatttt ccatattttg gtgattaccc tgcaggcgtt
     6301 tattttcatg gcgttggcgt tcgtctatat cggacaggca catgatgcgc actgattttc
     6361 ctaagtagtt tgtttttgta gtttaacctt tgtttcttta aggagtttaa aatgggtttg
     6421 attgctatcg catgtggttt gatcgttgca ttgggtgcat tgggtgcctc tatcggtatc
     6481 gcaatggtcg gttctaaata tttggagtct tctgcccgtc agcccgaact gatcggtccg
     6541 ctgcaaacca aactgttcct gattgccggt ttgattgatg ccgcattctt gattggtgtc
     6601 gccattgcac tgctgttcgc cttcgttaac ccgtttgcag gtgcataatc aggacggatt
     6661 cgtccgttcc gtacaggcgc agcctgtttg attaacccca gtacgaaggt taagtaacgt
     6721 gaatatcaat gcaacattat tcgctcaaat catcgtcttt ttcggtttgg tatggtttac
     6781 catgaaattc gtgtggccgc cgattgcaaa agctttggat gagcgtgccg caaaagtcgc
     6841 cgaaggcttg gctgccgccg agcgtggtaa aagcgatttc gagcaggctg aaaaaaaggt
     6901 tgcagaactc ttggcagaag ggcgtaatca ggtttccgaa atggttgcca acgccgaaaa
     6961 acgtgccgcc aaaattgtcg aagaagccaa agaacaggct tcttccgagg cggcgcgcat
     7021 tgcagctcag gcaaaggccg atgtggagca ggaattgttc cgcgcacgcg aatccctgcg
     7081 cgagcaggtt gccgtgttgg ctgtcaaagg tgccgaatct attttgcgca gcgaagtcga
     7141 tgcttccaaa cacgcaaaac tgctcgatac cctgaaacag gagctgtaat cttatggcag
     7201 agttcgcaac gattgccaga ccttatgcaa aggcattgtt cggtttggct caggaaaaaa
     7261 accaaattga gtcttggttg ggcggactgg aaaaacttgc ggcggttgtt caggaaggga
     7321 aggtggcttc attgattgac cgtcctgaaa cgaatgcttc agaaaaagca gatatcctca
     7381 tcgatttggt cggtttgaaa gacaaggagt tgaaaaactt tgttatcgtc ttggccgggc
     7441 agaaacgttt gtcgatattg ccggaagtat atgctcaata tcaagacttg accttatcat
     7501 tcaaccatat caaatctgcc gtcatttaca gtgcctatcc gttgaccgac aaacaggtcg
     7561 gcgagttggc gcaaatgctg aataagcgtt tcgacagcga gctgaaaatc tctgtcgaaa
     7621 tcgaaccgga gctgattggc ggcataaaag ttgaagtggg tgatcaggtt ttggatttgt
     7681 ctgtacaagg caaattgagt gccttgtaca cgactatgac gaattaggag agttttcatg
     7741 cagcttaatc ctgctgaaat tagcgatttg attaaagcca agatcgaaaa tctgtctgta
     7801 aatgccgaag tgcgtacccg tggtacggtg atttcggtaa ctgacggtat cgttcgcatc
     7861 catggtttgt cagatgcaat gcaaggtgag atgctcgaat tcccgggtaa cactttcggc
     7921 ttggcgatga acctggagcg cgactccgtc ggcgccgtag tgttgggcga gtacgaacat
     7981 attaaagaag gcgacacggt tacctgtacc ggtcgtatct tggaagtgcc ggtcggacgc
     8041 gaactggtcg gacgcgtcgt tgatgcattg ggtcggccta tcgacggcaa aggcccgatt
     8101 aatacaactt tgaccgcccc tatcgaaaaa atcgcaccgg gcgtgattgc gcgcaaatcg
     8161 gttgaccagc cgatgcaaac cggtctgaag gcgattgact ctatggttcc tgtcggtcgc
     8221 ggtcagcgtg agttgattat tggcgaccgt caaaccggta aaacggccgt agcgctggat
     8281 gccatcgtca accaaaaagg tacgggtgtt atctgtatct atgtcgctat cggtcaaaaa
     8341 gcatcttcta ttgccaacgt ggtccgcaaa ttggaagagc atggcgcgat ggaacacacg
     8401 attgtggttg ctgcaactgc atctgaagcg gcggcattgc aatatatcgc accttactcc
     8461 ggttgtacga tgggtgaatt cttccgcgac cgcggcgaag atgccttgat tgtttatgac
     8521 gatttgtcca aacaggctgt ggcttaccgt caaatttccc tgcttttgcg ccgtccgccc
     8581 ggccgcgaag cctatcccgg cgatgtgttc tacctgcact cccgtctgtt ggaacgtgca
     8641 gcacgtgtca atgaacacga agtggagaaa ttgaccaacg gcgaagtaaa aggcaaaacc
     8701 ggttctctga ctgctttgcc gattatcgaa acccaagcgg gcgacgtatc tgctttcgtt
     8761 ccgactaacg tcatttcgat taccgacggt cagattttct tggaaaccga cctcttcaac
     8821 gccggtatcc gtcctgcaat caatgccggt atttccgtat cccgcgtagg cggtgctgca
     8881 caaaccaaag tgattaaaaa gctgggtggc ggtatccgtt tggcgttggc gcaatatcgt
     8941 gaattggcgg cgttctcgca atttgcatcc gatttggatg aagctacgcg caaacaattg
     9001 gagcatggcg aagtcgtaac cgaactgatg aaacagaaac agttcagtac gttgaatacg
     9061 gctgaaatgg ctttgaccct ttgggcaatc aacaacggtt cgtattctga tgttccggtt
     9121 gccaaagctc tggcttttga atctgaattt ttgagctttg tccgtaccca acatccggaa
     9181 gttttggaag ccgtcaatgc ttcaggtgca atgtccgacg agagcgagaa aacgcttgaa
     9241 gcagccatga aatccttcaa atcttcttac gcctatcagg cataaggctt gaagtgaaag
     9301 gagtctgaaa tggcagtagg aaaagagatt ctcaccaaaa tccgcagtgt tcagaatacc
     9361 caaaagatca ctaaagcgat gcagatggtg tcaacctcta aaatgcggaa gactcaggaa
     9421 cggatgcgtt tggcgcgtcc gtatgccgaa aaagtgcgta tggtgatgag ccatcttgcg
     9481 caaaccaata ccgatcatgg tattccgtta ctggaatctc atcgggaaat cagacgtgtc
     9541 ggttttattt tgattacgtc tgataagggt ttgtgtggcg gtttgaacgc caacgtgctg
     9601 aaaaagtttt tggcacaagt tcaagagtat cggaatcaag gtattgaaga ggaaatcgta
     9661 tgccttggca gtaaaggtct gatggcgtgt cagagcattg gtctgaatgt ggttgccagt
     9721 gccgtaaatt tgggcgatac cccaaaaatg gaaatgctgc tcggaccttt gacagaactc
     9781 ttccaacggt atgagaaaca tgaaattgac agaatccatc tggtgtattc gggttttgtc
     9841 aataccatgc gtcaagaacc gagaatggaa gtattgctgc ctatcggtga gaacgtgatt
     9901 ggcgattcag ctcccaaatc accgttcagc tgggaatacc gctacgaacc gactgcactt
     9961 gcagtgttgg aatatctggt tcgccgctat ttagagtctg tggtttatca ggcgttgagc
    10021 gacaatatgg catccgaaca ggcagcccgc atggttgcca tgaaagctgc aacagacaat
    10081 gcaggcaatg ccatcaaaga gttgcgtttg gtatataaca aatcgcgtca agctgcgatt
    10141 accacggaat tgtcagaaat tgtagcaggt gcggcggctg tctgatgccg tctgaatcct
    10201 gtacggaaat taggatacga taatgagcca aggcaaaatc gtacaaatta tcggtgcggt
    10261 tgttgacgtg gaatttccac gcgacatgat tccgcgcgtt tacgacgctt tgaaattaga
    10321 cgaaaacggt ctgactttgg aagtccaaca gcttttgggc gacggcgtag tccgtaccat
    10381 tgcgatgggc agctcggacg gtttgaaacg cggcatgact gtgagcaata ccggtgcgcc
    10441 cattactgtg ccggtaggta aaggtacgtt gggacgcatt gtcgatgtat tgggaacgcc
    10501 tgttgacgag gcaggtccaa tcgataccga caagagccgt gccatccacc aagccgctcc
    10561 taagtttgac gaactgtctt ccacaaccga attgctcgaa acgggcatta aagtgattga
    10621 cttgctgtgt ccgtttgcca aaggcggtaa agtaggtctg ttcggcggtg ccggtgtggg
    10681 taaaaccgtg aacatgatgg aattgatcaa caacatcgcc aaagcgcaca gcggcttgtc
    10741 cgtgttcgca ggcgtgggtg agcgtacccg cgaaggtaac gacttctacc acgagatgaa
    10801 agattccaac gtattggata aagtggcaat ggtttacggt cagatgaacg aacctccggg
    10861 caaccgtttg cgcgtcgcat tgaccggttt gaccatggcg gaatacttcc gtgacgaaaa
    10921 agacgaaaac ggcaaaggcc gcgacgtatt gttcttcgtg gacaacatct accgttacac
    10981 tctagctggt accgaagtat ccgcattgtt gggccgtatg ccgtctgcag tgggctacca
    11041 accgacattg gcagaagaaa tgggtcgttt gcaggagcgt attacctcta cccaaaccgg
    11101 ttctattact tctatccaag ccgtatatgt acctgcggac gacttgaccg acccgtctcc
    11161 ggcaacaacg ttcgcccact tggacgcaac cgtcgtattg agccgcgata ttgcctcttt
    11221 gggtatttac ccggcagtcg atccgcttga ttccacttcg cgccaactgg atccgatggt
    11281 cttgggtcaa gagcactacg acgtggcgcg cggcgtacag tccaccctgc aaaaatacaa
    11341 agaattgcgc gacatcatcg ccatcttggg tatggacgaa ttgtctgacg aagacaaact
    11401 gaccgtaatg cgtgcgcgta aaatccaacg cttcctgtcc caaccgttcc acgttgccga
    11461 agtgtttaca ggttctccgg gcaaatatgt cgccctgcgc gataccattg ccggcttcaa
    11521 agccatcttg aacggcgaat acgatcatct gcccgagcag gcattctata tggtcggcag
    11581 cattgaagaa gcggttgaga aagcgaaaac cttaaactga ggaggtcggc atgagcatca
    11641 tgcaagttga ggtggtaagt agcgagcaga aaatctattc aggcgaggca acatttatcg
    11701 ttgttccgac tgtacagggc gaactcggta tttatccgcg acacgagccg attatgagtt
    11761 tggtgcggcc gggggctttg cgtttgaccg ttccgggcga ggataaagag gttttggttg
    11821 ctgtttccgg cggtgttttg gaagtacagc ctgataaagt aactgtcttg gcggatgttg
    11881 ccgtccgcag tgcagagatg gatcaggcac gtgcggaaga ggcgaaaaaa gccgcagaag
    11941 cgggcatttc ccaagctaaa gacgataagg ctttggcgga ggcgcataaa gcattggctg
    12001 ctgcgattgc acagcttaaa actttggact atatccgttc gcacaagaaa taaacttgtt
    12061 tggtttggaa aagcacgacc gtttggtcgt gctttttatt tttccaagtt ttgaaaatat
    12121 agtggattaa caaaaatca
//
LOCUS       DAWKQJ010000047        12076 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000047 DAWKQJ010000000
VERSION     DAWKQJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.047"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(43..972)
                     /locus_tag="WJL20_000978"
     CDS             complement(43..972)
                     /locus_tag="WJL20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7683145.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1103..1852
                     /locus_tag="WJL20_000979"
     CDS             1103..1852
                     /locus_tag="WJL20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ7683146.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1975..2556
                     /gene="petA"
                     /locus_tag="WJL20_000980"
     CDS             1975..2556
                     /gene="petA"
                     /locus_tag="WJL20_000980"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ7683147.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2575..3924
                     /locus_tag="WJL20_000981"
     CDS             2575..3924
                     /locus_tag="WJL20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ7683148.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            3939..4727
                     /locus_tag="WJL20_000982"
     CDS             3939..4727
                     /locus_tag="WJL20_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ7683149.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5066..6850
                     /gene="rmuC"
                     /locus_tag="WJL20_000983"
     CDS             5066..6850
                     /gene="rmuC"
                     /locus_tag="WJL20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ7683150.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7056..7688)
                     /locus_tag="WJL20_000984"
     CDS             complement(7056..7688)
                     /locus_tag="WJL20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ7683151.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7779..8603
                     /locus_tag="WJL20_000985"
     CDS             7779..8603
                     /locus_tag="WJL20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ7683152.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            8672..9235
                     /locus_tag="WJL20_000986"
     CDS             8672..9235
                     /locus_tag="WJL20_000986"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ7683153.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9316..9753
                     /locus_tag="WJL20_000987"
     CDS             9316..9753
                     /locus_tag="WJL20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ7683154.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9822..10091
                     /locus_tag="WJL20_000988"
     CDS             9822..10091
                     /locus_tag="WJL20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ7683155.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10091..11866
                     /gene="ptsP"
                     /locus_tag="WJL20_000989"
     CDS             10091..11866
                     /gene="ptsP"
                     /locus_tag="WJL20_000989"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ7683156.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVLQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         2946 a         3056 c         3393 g         2681 t
ORIGIN      
        1 atgaaaaaat agaaaaactg ccgggtatgg ttgaataagg ggtcagaccg gttccaattc
       61 gctcagtccg ggcaaatctg caaaaccgcg ttcgcgtatg atttggcaaa aattgttcag
      121 atagctcttg tccgtatctt cggtacggat ggcggcatac agtttgcttt gcaatccgtc
      181 ggcagtaatt tggcggtgga cgacatagcc tttttcaagg tagggcatga ctgtccaata
      241 gggaagggcg gcaatgccac gtctgctggc aaccagttgg ataatggcga tggtcagttc
      301 gctgtgtcgg cgcggcgggt tgatgttttt cggaatcagg atttttttgg gcaaatccag
      361 catctcgtcg ggaacgggat aagtaatcag ggtttccccg ataaagtctt ccgccgtcca
      421 aacgtttttg gcggcaagcg gatggtctgg tgcgcaaatg ccgaccattt cgtaggcaaa
      481 cagcggttgg aaagaaatat cgttttgttt ttccgcttcg gaaacaatgg caaggtcggc
      541 acggtgttgc agcagcagtc cgacgggatc cgcttggaat cccgatacga tatccaattc
      601 gacttggggc cacatcgggc ggaattcgcc catggcgggc atcagccagt cgaaacaggt
      661 atggcattcg acggcaatcc gcagctctcc cgcctctcct tccgtgattc gcgccaaatc
      721 ccattctgca acagcaactt gaggtataag ttcgtgggcg aggcgcagca gcctttcgcc
      781 caccggggta aagcgcaagg gcgtggattt gcgttcgaac agcggcgtgc cgtagtggtt
      841 ttcgagcata cggatctggt gggaaagagc ggattgggta aggaaaaccc gtttggcggc
      901 aagggagacg ctgccggttt cttcaagtgc cagcagggtc ttgaggtggc gcagttcgat
      961 aatggaatcc atggggcttc agacggcata ttgaaccggc gcatattaaa ataattcata
     1021 tgcgggtgca aatcggaaaa atgtgaattc cggacatttt gcgattagaa tgcccgcttg
     1081 tttaaagcga ttaggaaaaa agatggtatt gcgcagggat tttctgactt ggtgtaatga
     1141 aacattgcag acagcgttgt ttaaagatta cgcccctaac ggtttgcagg ttgaagggag
     1201 ggaatatatc gggaaaatcg ttacgtcggt aacggcaagc agggcagcga ttgattttgc
     1261 tgtggagcag aaggcagatt tgcttttggt acatcacggt atgttctgga aaaacgagtt
     1321 gccgaccgtt actggttgga aaaaagaacg gattgccgca ctgttacggc acgacatcaa
     1381 tatggcaggc taccatctgc ccctggatgc acatcccaca ctgggcaaca atgcccaact
     1441 cgccgacaga ttgggttttg cgacagaaaa acggttcggc gaacaaaacc tgctcaactc
     1501 gggcagcctg aaacaagcca agacactcgg cgcattggcg gcgcatattg aaacagtttt
     1561 gcaacgtaag cctgtcgtca tcggcaaacc cgaacgcgaa atccgacggg ttgcatggtg
     1621 cagcggcggg gcgcaggggt tttttcagac ggcaatagac gaaggtgtcg atctgtattt
     1681 gacgggggaa atctctgaag cccaatacca ccttgccaat gaaacgggta cggctttcat
     1741 ttcggcaggg catcacgcga cggaacgtta cggcgtacgc gcgctggcag aatcggcggc
     1801 agaggttttc gggttggaag tgtgccattt tgacgaaaac aatccggctt gagtcttgag
     1861 aaatatcata aaactttacc ttattttaaa taatgctttg agtatcactg caaactgggt
     1921 aaaattgcaa cgtttaatgg ctcggtattc ccaaaatatt aggacgactg aataatggat
     1981 aatcaagaaa tcaacaacgg ccgccgccgt ttcctgacac tcgcgacctg cggcgcgggc
     2041 ggagtggcag cattgggtgt ggcaacgccg tttgtggcca gttttttccc ttcggaaaaa
     2101 gccaaggccg ccggtgctgc cgtcgaggtg gatgtcagta aaatcgaagc gggtcagctg
     2161 ctgaccgccg agtggcaagg caaaccgatt tgggtgctca accgtacaga tcagcagctt
     2221 aaagacctga aaggcctgaa cggcgaactt accgatccca attccgatgc ggaacagcag
     2281 ccggagtatg ctaaaaacga gacccgttcg attaagccga acatccttgt cgccatcggt
     2341 atctgcaccc atttgggctg ctcgcccacc ttccgtcccg acattgcccc cgccgatttg
     2401 ggtgcagact ggaaaggcgg cttcttctgc ccctgccacg gttcgaaatt cgacttggcc
     2461 ggccgcgtat ataaaggtgt tcctgccccg accaacctgg ttgtcccgcc atataaatac
     2521 ttgagcgaca caactatctt ggtgggcgaa gactaagaat aaggaacgat aattatggca
     2581 aaccaaacca atagcaaagc aaaagcattg ttaggctgga tggacgctcg tttccccctg
     2641 tctaaaatgt ggaatgagca tttggctcaa tactacgcac cgaaaaactt taatttttgg
     2701 tactacttcg gctctttggc cctgcttgtc ctcgtgattc aaatcgtcag cggcattttc
     2761 ctgaccatga actacaaacc cgacggcaac ctgaacgcct accatctgcc tgctgccttt
     2821 actgcagtcg agtacatcat gcgcgacgtg tccggcggtt ggattatccg ctacatgcat
     2881 tccaccggcg catctttctt cttcattgtt gtttatctgc acatgttccg tggtttgatt
     2941 tacggttcgt acaaaaaacc ccgtgagttg gtgtggattt tcggttctct gattttcttg
     3001 gcattgatgg cggaagcctt tatgggctac ctgctgcctt ggggtcagat gtccttttgg
     3061 ggggcgcagg taattattaa cctgttctcc gccatccctg ttatcggtcc ggatttgtcc
     3121 acttggattc gcggcgactt caacgtttcc gacgttaccc tgaaccgttt ctttgccctg
     3181 cacgttatcg cggtacctct ggtattgctc ggcttggttg tggcgcacat catcgccctg
     3241 cacgaagtgg gttctaacaa ccctgacggc gttgaaatca agaaaaacaa agacgaaaac
     3301 ggtatcccgc gcgacggtat cccattccat ccttactata ccgttaaaga catcttgggt
     3361 gtggttgtat tcctgattgt cttctgtgcc gtattgttct tcgcaccgga aggcggcggc
     3421 tacttcctcg aagcgccgaa cttcgatgcg gcaaatgcgc tgaaaacacc cccgcacatt
     3481 gcgccggtat ggtatttcac accgttctac gccattttgc gtgcgattcc ctcgttcttg
     3541 ggaacgcagg tttggggtgt aatcggtatg ggcgcggcgg tcgtgttgat tgccctgctg
     3601 ccttggctgg ataggggcga ggtgaaatcc gtccgttacc gcggtccgat tttcaaaacc
     3661 gcattggtct tgttcatcat ttcctttatc ggtttgggta ttttgggcgc gatggtggca
     3721 accgatacgc gtactttggt cgcgcgcatc ctgtccttcg tctatttcgc attcttcctg
     3781 ggtatgccgt tctataccaa gctggataaa aacaaaccgg ttcccgaacg cgtaaccatg
     3841 agcaccgcca aacaaaaaat catgttcttt gtttacgtcg gcattaccgc cgtgggtgct
     3901 tacctgtttg caaccaacat ctgatgaggg cagtcaaaat gaaacaagct atgaaaaatt
     3961 ggtttgctgc cttgctgttg gcagtaccca tgggtgcggc atttgcttcg ggcggtcatg
     4021 cacactatga gaaagtcgat atcgatttgc gcgaccaagt cagcctgcag cgcggcgcac
     4081 aaatctttac caactattgc ctgtcctgcc actcggcaag cggtatgcgt ttcaaccgtt
     4141 tgaaagacat cggtttgact gacgaagaaa tcaagaaaaa cctgatgttt accaccgata
     4201 atgtcggcga tgtcatgcat tcggcgatga acccgaaaga tgcggcaaaa tggtttggtg
     4261 ctgctccgcc cgatttgaca ttgattgcgc gttccaaagg tgcagactac ctttacgctt
     4321 atatgcgcgg cttctataaa gatccgaccc gtccgagcgg ctggaacaat actgtattcg
     4381 ataaagtcgg tatgccccac ccgttgtggg aacagcaagg cgttcaagcc gttgagttgg
     4441 atgccaaagg tcagccggtt atggtaaaag acgaacacgg cgagatgaag cctaagctgt
     4501 attgggaatc taccggtttg cacagccgcc gtctgcctaa cggcaaagtg atccaaaaag
     4561 agtacgacgc atatgtacgc gatttggtca attaccttgt gtacatgggc gaacctgcac
     4621 aactgcaacg caaacgtata ggctatgtcg tgatgatttt cctatttgcg gttatgctgc
     4681 ctttggcgta tttcctgaaa aaagaatatt ggaaagacgt acactaagcg tttggaacaa
     4741 aagggcaaat cctttagggt ttgccctttt ttcattttgc ctgccgtttg aaaagcctaa
     4801 atccgtatgc cgtctgaaca tagctgcaac atttcagacg gcatcattct aaaaatgtca
     4861 gacatcggga acttaatcag ggtttgtggc agattgatat tgaattagga aaaggtctga
     4921 aaccgcaagc agcatggtaa ggcgatgtcg aacaggttta aagccaatcc gctatatttt
     4981 ttggagctat gtcgaacaaa caggtagaat cggaataagc ggcggttttg tccggtgtga
     5041 aaatgtttga atattaggat gattgatgga actgatgact gttttgctgc ctttggcggc
     5101 gttggtgtcg ggcgtgttgt ttacatggtt gctgatgaag ggcaggtttc agggcgagtt
     5161 tgccgatttg aacgcgcaac tggcggaaaa ggcggcaaga tgtgattttg tcgaacaggc
     5221 acacgcagaa attgcatcgg aattggccgt tttggacggg aaataccgac atttgcagga
     5281 cgaaaattat gctttgggca accgtttttc cgcagccgaa aagcagattg cccatttgca
     5341 ggaaaaagag gcggaatcgg ttcggctgaa gcagtcgtat atcgagttgc aggaaaaggc
     5401 acagggtttg gcggttgaaa acgaacgttt ggcaacgcag ctcggacagg aacggaaggc
     5461 gtttgccgac caatatgcct tggaacgcca aatccgccaa agaatcgaaa ccgatttgga
     5521 agaaagccgc caaactgtcc gcgacgtgca aaacgacctt gccgatgtcg gcaaccgttt
     5581 tgccgcagcc gaaaaacaga ttgcccattt gcaggaaaaa gaggcggaag cggagcggtt
     5641 gaggcagtcg cataccgagt tgcaggaaaa ggcacagggt ttggcggttg aaaacgaacg
     5701 tttggcaacg caaatcgaac aggaacgcct tgcttctgaa gagaagctgt ccttactggg
     5761 cgaggcgcgc aagagcttga gcgatcagtt tcaaaatctt gccaacacga ttttggaaga
     5821 aaaaagccgc cgttttaccg agcagaaccg cgagcagctc catcaggttt tgaacccgct
     5881 aaacgaacgc atccacggtt tcggcgagtt ggtcaagcaa acctatgata aagaatcgcg
     5941 cgagcggctg acgttggaaa acgaattgaa acggcttcaa ggattgaatg cgcagctgca
     6001 cagcgaggca aaggccctga ccaacgcgct gaccggcacg cagaacaagg ttcagggtaa
     6061 ttggggcgag atgattctgg aaacggtttt ggaaaattcc ggcctccaga aagggcgcga
     6121 atatgtggtt caggcggcat ccgtccgaaa agaggaagac ggcggcacgc gccgcctcca
     6181 gcccgacgtt ttggtcaacc tgcccgacaa caagcagatt gtgattgact ccaaggtctc
     6241 gctgacagct tatgtgcgct acacgcaggc ggcggatgcg gatacggcgg cacgcgaact
     6301 ggcggcacac gttgccagca tccgtgcaca catgaaaggc ttgtcgctga aggattacac
     6361 cgatttggaa ggtgtgaaca cattggattt cgtctttatg tttatccctg tcgaaccggc
     6421 ctacctgctg gcgttgcagc atgacgcggg attgttccaa gagtgtttcg acaaacggat
     6481 tatgctggtc ggccccagta cgctgctggc gactttgagg acggtggcga atatttggcg
     6541 caacgaacag caaaatcaga acgcactggc gattgcggac gaaggcggca agctgtacga
     6601 caagtttgtc ggcttcgtac agacgctcga aagcgtcggc aaaggcatcg atcaggcgca
     6661 aagcagtttt cagacggcat tcaagcaact tgccgaaggg cgcgggaatc tggtcggacg
     6721 cgccgagaaa ctgcgtctgt tgggcgtgaa ggcaggcaaa caacttcaac gggatttggt
     6781 cgagcgttcc aatgaaacaa cggcgttgtc ggaatctttg gaatacgcgg cagaagatga
     6841 agcagtctga cttgtgcgga aaagcttgtt tcagcagggg cgggaatgcc gaaagcgcgg
     6901 cgtaactgta ccggttctga tttggggttt gtttgaagta cttaccaaag ccttgtccgg
     6961 cgaggtacac cggcaaggcg gcggttgtct gagccttttg cgtttcagac ggcatcagtg
     7021 cagatgacgc cgtctgaaga ccgtagtgaa gtcggttaga aaaacggatt gtgccgtttt
     7081 tcgtgtccga tggaagtcat acgcccgtgt ccggcgacaa cttgcacggt ttcggggagg
     7141 gcgaataatt tgttgcggat attattgatt aagtcggcgt ggttgccgcg cggaaaatcg
     7201 gttctgccta tggtttcgta aaacaacacg tcgcccgcaa tcagcaattc cgcctcggca
     7261 caataaaaga cgatatgtcc cggcgtatgg cccggaatat gcagcacttg aaaggcatag
     7321 cgtccgaccg tgagcgtttc gccttcttcg agccaacggt tcggcgcaaa ggcgggcgag
     7381 acgggaaatc cgtattgcgc ggtggtttgc ggcagcgatt ggagcaggaa ttcatcgtcc
     7441 ggatgcggcc cgaggacagg gactttatgc gttttcaaca tttcgaccac gccgcccgcg
     7501 tgatcgagat ggccgtgcgt cagccagatt gccgtgagcg taagtttgcg gtttgccaac
     7561 gcttgcagca ggaacggcac gtcgccgccg acatcggtca ggacggcttc gccgctttcg
     7621 tcgtcccaaa tcagggtgca gttttggcgg aagggggtaa cggagaggat ttcgtaacgt
     7681 agggtcatgg cgtgttccta aacggttttt cagacggcat cgggtttgcc gtttgtttta
     7741 tggcggtttg ccgcctgttt tgatattggg ggaatcagat gattaagaag acaatcggcg
     7801 gcatcatacc gatttttacg gcggttttca tccctgcatc agcaggcgcg gcggatttga
     7861 tgctggcgca ggaatacaaa gaccaagaga ttgcaggctg ggcaatgagc gagaaactcg
     7921 acggcgtgcg cgcctattgg gacggaaagc acctgataag ccgtcagggc tacgcgttta
     7981 cgccccccaa aggctttacc gcgcagtttc cgccttatcc tttggacggc gaattgtata
     8041 gcggacgcgg tcagttcgag cagatttccg ctaccgtgcg ttctgtttct tcagactggc
     8101 gcggcatccg cctgcacgtt ttcgacgtac ccaaggcgca gggcaatctc taccaacgtt
     8161 tggcagtcgc aacgcagtgg ctgaaaacgc atccgaacgc gccgattacc atcatcccgc
     8221 aaatcaaagt gcgcgaccgg cagcacgcga tggacttttt aaaacaaatc gaagcgcagg
     8281 gcggcgaagg cgtgatgctg cgccagcccg aatcccgtta cagcggcggc aggagcagcc
     8341 aattattgaa gctgaaaagc caatacgacg acgaatgcac ggtaacgcgg cactatgagg
     8401 gcaaagggcg aaacgccgga cggttgggcg cggtcggctg caaaaaccgg cacggcgaat
     8461 tccgcatcgg cagcggtttc aaagacaaag accgcgacaa cccgcccaaa atcggcacac
     8521 tgattaccta ccgctaccgt ggctttacgc agaaaggcac gccgaaattc gccacgtttg
     8581 tgcgcgtgcg taccgaccgc tgagcaggac agttcagacg gcatccgata ctttggttta
     8641 taattttcct tttaccgacc gattccgaca tatgaccgat ttagaaacca aacgccttga
     8701 aacacaggcg atgcttgaaa acgccgatct tttgttcgac caaggccaat gccgtgccgc
     8761 actgcaaaaa gtggcggacg agattacgcg cgatttgggc ggcaaatatc cgctgctgct
     8821 gcccgtgatg ggcggcgcgg tggtgtttac ggggcagttg ctgccgctgt tgcgttttcc
     8881 cttagatttt gattatgttc acgtttcccg ttacggcgac aagctggagg gcggcgcgtt
     8941 caactggaag cgtatgcccg atgcggaaca aatccggggc aggcacgtcg tcgtgctgga
     9001 cgatattttg gacgaagggc atacgatgtc cgccattcaa gccaaacttt tggaaatggg
     9061 tgcggcaagc tgccgtgcgg cggtgttcgc caacaagctg attgacaaac ccaagcctat
     9121 ccgagccgat tatgtcggac tggatgtgcc gaaccgttat gttttcggtt acggcatgga
     9181 tgcggcgggc tgctggcgca atttgggcga gatttacgca ttggacggaa aataaggacg
     9241 cgatgccgtc tgaaggctgt tcagacggca ttgcgcccat acgccggcag gacaacgagg
     9301 aacaggacgc ataatatgat agggctttta atcatcacac acgaaaccat aggcgaagcc
     9361 taccgcaagc tggcacatca tttttttccg ggcggactgc ctgaaaacgt ccgcatactc
     9421 ggcgtgcagc cgacggaaga ccaagacgac atcatcaaca acgccattgc cgcgcttcag
     9481 gagtttcccg acaacgacgg cgtgctaatt atgaccgata ttttcggcgc gaccccctgc
     9541 aatgcagccc gccgcctcgt gcgcgaaaac aaatcggcga ttttgaccgg gctgaacgcg
     9601 ccgatgatgg ttaaggccgt ccaatattcg ccggcggcgg aagaccttgc cgcctttacc
     9661 gaatgcgtca gggaggcggc tgtaaaaggc attttcgcca tcacgtccgc gcccgaagat
     9721 ttggtgtgcc ggcgcagcgg cgatgccgtc tgaagaagcg gcagggcagg aaaacatttt
     9781 aaacggcgtc tgctgccgat atacataaca cgggaaccga aatgctcaaa caatccatcg
     9841 aaatcatcaa caaactcgga ctccacgccc gcgcgtccaa caagttcacc caaaccgcgt
     9901 cccaattcaa aagcgaagtc tgggttacga aaaacgacag ccgcgtcaac ggcaaaagca
     9961 ttatggggct gatgatgctc gccgccgcca agggtacggt catcgaactg gagacggacg
    10021 gcgcggacga ggcggaagcg atgcgcgccc tgaccgactt aatcaacggc tacttcggcg
    10081 agggcgaata atgagtatcg tgctgcacgg cgtggcggcg ggcaaaggca ttgccgtcgg
    10141 ttgcgcccac ctgattgcgc gcggtacgga ggaagtgctg cagtatgatg ttgcggaggc
    10201 ggacaccgat gccgaagccg aacgtttcga tgccgccgtc aaagccacgc gcaaagagtt
    10261 ggaacagctc cgcagcgcga ttcccgaaaa cgccccgacc gagttgggcg cgttcatctc
    10321 gctgcacctg atgctcttga ccgatgttac cttgtcgcgc gaacccgtcg atattttaag
    10381 ggaacaaaaa atcaacgccg agtgggcatt gaagcagcag agcgacaaac tcgccgccca
    10441 attcgacaat atggacgatg cctatttgcg cgaacgcaag caggatatgc tgcaagtcgt
    10501 ccgccgcatc cacaacaacc tgatcgggca gggcaacgag ttggaagttg ccgacaacct
    10561 gtttgacgaa accgttctga ttgcaaacga cctttcgccc gccgacacgg ttttgtttaa
    10621 agagcagcgc attgccgcct tcgttaccga tgccggcggc cccaccgggc atacggcgat
    10681 tttgggcagg agcttggaca tcccgtccgt cgtcgggctg cacaacgcgc gcaaactgat
    10741 taccgaaggc gaaacggtca ttgtggacgg tatcaacggc gtgttgatta tcgcgccgga
    10801 tgagtcggtg ttgaacgaat accgccgccg tgcccgcgaa taccgcagcc acaaacgcga
    10861 tttgaacaag ctcaaaaaaa ccgccgccgc caccgccgac ggggtctgca tcgagcttgt
    10921 gggcaatata gaatccgccg aagacgtgaa accgctgcac aacctcggcg cagacggcat
    10981 cgggctgttc cgcagcgagt ttctttacct gaaccgcgat acgatgccgt ctgaagacga
    11041 gcagtacgaa gtgtacagcg cgattgtcaa aaaaatgaaa ggcaaaagcg taacgatacg
    11101 gacagtcgat ttgggtgtgg acaaaaaccc gcgctggttc gggaaaaaca gcacgcccaa
    11161 cggcagcctc aaccccgcgc tgggcatgac cggcatccgc ctgtgccttg ccgaaccggt
    11221 catgttccgc acccagatgc gcgccatcct ccgtgcggcg gtacacggcc ccgtgcggat
    11281 gatgtggccg atgattacct cagtatccga agtgcgccag tgcctcatcc acctcgacac
    11341 cgcgcaacgc cagcttgccg aacgcggcga tgccttcggt aaagtcggca tcggctgtat
    11401 gattgaaatt ccgtctgccg cgctgaccgt cggcagtatt ttgaaactgg tcgatttcat
    11461 ctccgtcggt accaacgacc tgattcaata catcttgtcc gtcgatcgcg gcgacgacag
    11521 cgtcagccac ctctaccagc ccggccatcc cgccgtgctg aaaatgctgc aacacgtcat
    11581 ccgtaccgcc aaccgcatgg acaaagacgt atccgtatgc ggcgagatgg cgggcgatac
    11641 cgcgtttacc cgcgttttat tgggtatggg gctgcgccgt ttttccatga accccaacaa
    11701 catcctgccc gtcaaaaaca tcattctgca cagcaatgtc ggacagctcg aaaacgagat
    11761 tgccaaaatc gtccgttgcg aggacgagga aaaggcggaa aagctgatca aacagatcaa
    11821 cagcgtgtct gtcgaggaag aagccgactt caaggggcgg aaataaatac ggcaggtaaa
    11881 aaatagaaat acttaacaat gcccgcaatc tgaaattttg ccattcttgc aaaatagaaa
    11941 accgaaacag aaacccaaaa tcggccattc cctcaaaaac agaaaaccaa aatcagaaac
    12001 ctaaaatccg tcattcccgc gcaggcggga atctaggtct gtcggcacgg aaacttatcg
    12061 ggtaaaacgg tttctt
//
LOCUS       DAWKQJ010000048        11792 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000048 DAWKQJ010000000
VERSION     DAWKQJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.048"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            100..382
                     /locus_tag="WJL20_000990"
                     /pseudo
     CDS             100..382
                     /locus_tag="WJL20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(403..1629)
                     /locus_tag="WJL20_000991"
     CDS             complement(403..1629)
                     /locus_tag="WJL20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ7683157.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1644..2069)
                     /locus_tag="WJL20_000992"
     CDS             complement(1644..2069)
                     /locus_tag="WJL20_000992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683158.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2174..2833
                     /gene="queC"
                     /locus_tag="WJL20_000993"
     CDS             2174..2833
                     /gene="queC"
                     /locus_tag="WJL20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ7683159.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2861..3379
                     /locus_tag="WJL20_000994"
     CDS             2861..3379
                     /locus_tag="WJL20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ7683160.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3386..3808
                     /gene="queD"
                     /locus_tag="WJL20_000995"
     CDS             3386..3808
                     /gene="queD"
                     /locus_tag="WJL20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ7683161.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            3917..4273
                     /locus_tag="WJL20_000996"
     CDS             3917..4273
                     /locus_tag="WJL20_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683162.1"
                     /translation="MNTTSSMTQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            4523..4894
                     /locus_tag="WJL20_000997"
     CDS             4523..4894
                     /locus_tag="WJL20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ7683163.1"
                     /translation="MKLLSTILVLLVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4891..5529
                     /locus_tag="WJL20_000998"
     CDS             4891..5529
                     /locus_tag="WJL20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ7683164.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5864..5940)
                     /locus_tag="WJL20_000999"
     tRNA            complement(5864..5940)
                     /locus_tag="WJL20_000999"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5904..5906),aa:Pro,seq:cgg)
     gene            complement(6165..7250)
                     /gene="nagZ"
                     /locus_tag="WJL20_001000"
     CDS             complement(6165..7250)
                     /gene="nagZ"
                     /locus_tag="WJL20_001000"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ7683165.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(7307..8827)
                     /locus_tag="WJL20_001001"
     CDS             complement(7307..8827)
                     /locus_tag="WJL20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ7683166.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(9027..10526)
                     /locus_tag="WJL20_001002"
     CDS             complement(9027..10526)
                     /locus_tag="WJL20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ7683167.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVTEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10664..11293)
                     /gene="nth"
                     /locus_tag="WJL20_001003"
     CDS             complement(10664..11293)
                     /gene="nth"
                     /locus_tag="WJL20_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ7683168.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(11339..11752)
                     /locus_tag="WJL20_001004"
     CDS             complement(11339..11752)
                     /locus_tag="WJL20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7683169.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2843 a         3071 c         3235 g         2643 t
ORIGIN      
        1 cgggattggt gtgggattta gggctaatct agtacagccc cttgtttttt cgatacggaa
       61 ccggatagag gaaaaatcga acattgcgcc tgccttgcta tgattcacga agaaatctcc
      121 gccatgccta tgggctatga aaccttgatc ggcgatatgg gcagcgcact gtcaggcgga
      181 caaaaacaac gcatcgtatt ggcgcgggcc ttaatattgc gaaccgaaaa tcctattttt
      241 agatgcagcg accagccatt tggatattgc caatgaaaaa gcagtcaatg caaacttgaa
      301 tggcttgtct atcataaaaa ttatggcggc acacagaaag gaaacggtgg aatcagcaga
      361 taggaaaatg tctttaggat aaaaatacag tttcaaaaat actcaagatt gctgctgttt
      421 tttcgcctga gcgtcaaact ctgccagcgt catgttcaaa gtctgcaaac acggcatcat
      481 tactgcatcg acagcttggt tcacatgatc cctttccaca ggcaacggac ggtaaacgaa
      541 gagcttgaag agttcgttca actcaatcga atccgccccc gttttcaaca cccaaccctg
      601 tctgccggaa tagatgtagc cgtgccgcgc caacttttcc aagagttcgc ccaactcgtc
      661 gtagcccata ttgatatgcc gtctgaactc ctgaacaggc aaggctttgc cttctttttg
      721 cgccgcatcc agaagcagca ggattttcaa cacgtcgtca aaccgtccgc gcgagtcgaa
      781 gcccctgcgg aacgcttctc cctgccagta ggagagtgaa gaagtcagca ccgcgccgcc
      841 caagaccagc gtccacaaca ggttcagcca caacagaaaa aacggcacgg cggcaaacgc
      901 gccgtaaatc gagcggtagc cgtcgaaatt gcccatatac caagtgaaga gggagcgcgc
      961 ggtttccaga caaaacgctg ttgccaaagc cccgacaaac gcctgccgcg cgggaacgaa
     1021 gcggtttggc acgaagcggt acagccccca cagcaaaagc gtcatgaagg tcagcgtcgc
     1081 cgccgttcgc aacgcgcccg accactgcgg cgcacctgag gcaagcgcgg catcctgtac
     1141 cgagccgacc ataaaggaaa tgcccacgcc caaagacagc ggcccgaacg tcagtaaagc
     1201 ccaatagacg agaaactgca tcatccacgg acgctgggaa ttgacccgcc agatgcggtt
     1261 gaacgtattg tctatcgtcc gaatcagcat cagcgaggta acgaccagca tcacgctgcc
     1321 gattgccgtc agccggttcg cctgctcgcg gaacgcattg atatagtcga acaccatgtc
     1381 cgcgccctgc ggcacaatgg tttggttgac gaaggagacg aacgaatccg accagcggtc
     1441 gaacacgggg aaaatcgaag cgaccgccac catcacggtc agcacgggga cgagtgccag
     1501 cagcgtcgta aacgtcatgc ttgccgccgc ctgcggtacg cgttcttcat caaagcggcg
     1561 gacgacgaac catgcaaacg cacagatttt attgtctgcc aaaccttgca aacgttgtaa
     1621 aaaggtcata atttcttgcc cggtcagtaa gttgggcatt gatgcccgat gttatagcca
     1681 attttgccgt caggaacaaa tgcctgaact gcggctgttt cagacggcat cggaacaact
     1741 gttatgccgt ctgaagaccg aaccatttta acggaatccg cccatgaacc caaatccccc
     1801 tcaaaatcct cgtcctctac tatttccaaa acggcagcac ccgcaatccc gcactccgaa
     1861 tcgctcgcgg catcgacagc gttgaaggtt gcgaagccgt attgcgcacc gtccccaaag
     1921 tctccgccgt ctgcgaagcc gtcaaaaaag atattcccga cagcggctcc cgtcctgacc
     1981 gccgaagaaa acaatatcgc cttcgcacaa agcaaacgct tggcggaact cgccgtcaag
     2041 tcggcataag ccgcgtgttc agacggcatg gcgttcagat gccgtctgaa cgcgtttgcc
     2101 tgtataatcc gcgtctttac tgtccaactt cgcggttcgc aaacctcccg cgttaccaaa
     2161 actaggattc gatatgtcaa accaaaaagc cttggtcatc ttttcgggcg gtcaggattc
     2221 gaccacctgc ctgattcagg caatccaaat ctacgggcgc gaaaacgtcc aagccattac
     2281 tttccaatac gggcaacgcc atgccgtcga gctggaacgt gccagctgga ttgcgcagga
     2341 tttgggcgtc aaacaaaccg tactcgactt gagcctgatg cggcagatta cgcacaatgc
     2401 cctgatggac gacaccgccg ccatcgaaac tgccgaaaac ggcgttccga atacctttgt
     2461 agacggccgc aacgcgcttt tcctgctcta tgccgcgatt tacgccaaag ggcaggggat
     2521 acggcacatc atcgcgggcg tgtgcgaaac cgacttctcc ggctatcctg actgccgcga
     2581 cgtgtttgtc aaatcgatga acgttaccct taatttggcg atggactatg attttcaaat
     2641 ccacacgccg ctgatgtatc tgaccaaggc gcaaacttgg gcgttggcgg acgaaatggg
     2701 cgcgctggac tatatccgcg agcaaaccca cacctgctat aacggcatcg tcggcggctg
     2761 ccgcgaatgc ccgagctgta tcttgcgcga acgcgggctg gcggaatatc tggaaagtaa
     2821 aaaggccgtc tgaacacgcg caaaccataa ggaatacgat atgcccaagc tccatatgtt
     2881 ttacctcggc ggcaatgccg gcaggtcgaa tatcgaagtg cacgacatcc aatttgccgt
     2941 gtgcgacgac taccgtgaag ccgtccccgc actcaaagcc gcgtggttcg gcgatgcgga
     3001 caaaatccac atcgacggct ggcaggttgt cgaatgggcg gacggttacg acatcgccgt
     3061 atccgaaacg cccaaaacga aaatgccgtc tgaacacgcc ccgcgcctgt atttcgccaa
     3121 tgtcggcggt tatcgcgcag ggcagctcgc cgaagcgcac gcttttgggc tgttcgccgc
     3181 cgccacgcct gccgaagcca aacaaaaagc cctgcaaacc ctgttgaccg acagctatgt
     3241 tcagcaacat aaagacaact taaaagacgt ggacaacctg cttgcgctcg accgcatcgg
     3301 caatttccat atccgcctga ccccgaatcc gcacggcaaa cccgccgaaa tcggctttca
     3361 aggctatttg cccatttgag aacccatgaa aatcaccaaa atcttcacct tcgactcctc
     3421 gcatatgctc gacgggcatg acggcaaatg ccaaaacctg cacggacata cctacaaact
     3481 cgaaatcacc gtttcagacg gcattatcaa aggcggcgcg aaagacggta tggtgatgga
     3541 ctttaccgac ttgaaagcca ttgtcaaaca acacattacc gaccccttcg accacgcctt
     3601 catctaccac ggcggcaaca gccgcgaatg ccaaatcgcc gcgcttttgg agggctggaa
     3661 catgaaaacc ctgcgcctgc cctgccgcac cactgccgaa aatatggcgg tcgaaatgta
     3721 cgaccgtctg aaaaacgcgg ggctgaacgt gtgccgcgtc aaattgtggg aaacgccgac
     3781 atcgtgtgcg gagtatgaag gggagtaata ttgaaacaaa ttttaaagat ttgttttgac
     3841 ttgtcatata atcaattgta aaattccata ctttctaaaa aatcgaccgt aatagaaaat
     3901 ttcaggcata ataaccatga atacaactag tagtatgact caagaggaag gcattattgc
     3961 cgagagcata gatgtaatta ataagttcgt acaatttctt ttgaaactgt acgatgattt
     4021 tgggattgat ggtatgcatg atttagttga tcctgatttg gatactttgg aatccattgt
     4081 gaaaaattta caacaagagg ttgataaact tccgatcagt cccaatgatt tttctttgga
     4141 aaacaaaaaa attagtttgg ctcaaggact tctctacgca caatctatga ttactaacgt
     4201 tagaaataaa gatactgagg aatgttctcg gaatcgttct atgctcaaaa ataatcagtc
     4261 gagcctttat taacaacagc aaggagttat ttatgaaatc aatggatatc cgtttcgctc
     4321 gcattgctgc attaacgaag caacttgcag ctattcaaag acaaattgaa aaacgagctt
     4381 ataaagcaaa agctgcaacc atgaaaaaag tagcatgaaa agttttggta gagaagccat
     4441 ccataatggg tggctttttt gttaaataaa attggtaaag tttgttaaat ataaatctta
     4501 gtgcaaatta aggtgcttac ccatgaaact cctctccacc attctcgtcc tccttgtcgc
     4561 cgtcgaacat ttctacatcg cctggcttga aatgacacag attcccagcg aaaaagcggc
     4621 ggaaacgttc aagctgcctt atgaatttat ggaacaaaat cgcgtgcaga ccctgttcgg
     4681 caaccaaggg ctgtacaacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc
     4741 gccggacaac gcggtgtacg gcgcaacggt actgtttctc ggcttcgtcc tgattgccgc
     4801 cgcgtggggc gcgttctctt ccggcaacaa aggtatactc gtcaaacaag gtttgcccgc
     4861 atttttggca gcggcggcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat
     4921 ccccaatacc gcatcgtcga aatctttgaa agcctgcaag gcgaaggctg gaacacgggc
     4981 atgcccgccg ttttcgtccg cttgggcaaa tgcaacctcg cctgcggctg gtgtgatacc
     5041 gattatttga cattcggcat gatgagcctg tccgacatct taggctgtct gaaaacctat
     5101 gccgcccgca acatcatcat caccggcggc gaaccgacca tacagccgca tctcgatatg
     5161 ctgctggaca cgctcaaggc ggaaggctat ttcctctgtc tcgaaaccaa cggactcaat
     5221 cccgcgccgc cgcaaatcga ctacgtcgcc accagcccca aagcctgcta cgccgccaaa
     5281 tatgaaaata gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc
     5341 cttgcgttct gcgaaaacat ggaacgcaaa atccgcgcac atcattacta cctttcgccc
     5401 tgtgagcaag acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattttaaac
     5461 agtcgccccg acgcatccgt gcattggcag ttgagcgtgc agacgcacaa atgggcggga
     5521 atagagtagt ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac
     5581 atacggggaa agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg
     5641 acggagcctt actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga
     5701 tggtcttttg aaccttgccg atactctgtg ccagtgcgcg caaatggcag ggttagggaa
     5761 aacgaaatgc cgtctgaaac agcattctgt ttcagacggc atttttctgt tgacgtcaaa
     5821 aggaaaaact gcctcggcaa tggataccga ggcagtttgg atttggtcgg aatgagagga
     5881 ttcgaacctc cgaccccttc gtcccgaacg aagtgcgcta ccgggctgcg ctacattccg
     5941 aattgattgg aacgggatta taacgcaaaa agtgcggcgt gcctataccg ttttgccttt
     6001 ttgccgcgtg tcgggcggat ttaaaacgtt gtgtttgaat acagtgttga taagcatcct
     6061 tatctccaag taattcaata agataacttt ctacctgacc gaaaaaatca ttgcctttcc
     6121 ctgacaaacg gttgaaatcg gcagattgtt gaaacgcagc cgatttaaaa ggcctctccg
     6181 acttttacgc cgcccgccgt gtcttgcggc gaggcaagac cggcaacaaa ggcttgcgcc
     6241 gcttggaaat ccgccgtctg tataacggct tgcgcggcgg cactgccgag cgtgttggcc
     6301 atatattgcc aacgttgcgc caaagcggga ttgtcaggaa tgcggaaatc ttcgcgcagt
     6361 tcatccacaa ggtcgggacg gttgcagacg aggacgatgt cgcaacctgc ctcaaaggaa
     6421 atgcgggcgc gttctttgat gccgcctgcc ccgcacgcgc cttccatagt caaatcgtcc
     6481 gagaaaatca cgcctttgaa cccgatgtcg cggcgcaaaa tttgtttgag ccagatttcg
     6541 gaaaacccgg cgggctttgt gtccacttgt ggataaacga cgtgggcggg cataaccgcc
     6601 gccatacctt cgcggctcat aatgcggaag ggaatgaggt cggcggcttc gagttcgtcg
     6661 aggctgcgcc cgtcttcggg tagcaccaaa tggctgtccc cttcgacaaa cccgtgtccg
     6721 ggaaaatgtt tgccgcagga tttcataccg ccttttgcca aacctttttg aagggcgagg
     6781 gcgaggcggg ttacggcttc ggggttactg tggaaactgc ggttgccgat gacggggcag
     6841 tttccccagt ccaaatctaa gacgggcgtg aaggacaagt cgatgccgca ggcggaaagc
     6901 tcggttgcca aaacccagcc gacttgtccg gcggcggttt cggcggcgga tgcgccgtct
     6961 ttgtcccaaa tttgccctaa aacgttcatt gcgggcaggc gggtgaagcc ttcgatgaaa
     7021 cgttgcaccc ttccgccctc gtgatcgacg gcgataatga gttcgggtgt gcgaagtgct
     7081 ttgatttcgg cggtgagtgt tttgagctgt tcgatgtttt ggaagttgcg gcggaagagg
     7141 atgatgccgc cgattgcggg gtcgagcagg cgttgttttt cttctccggt caggcggaag
     7201 gcggcaatgt ctgccatgac ggggccgcgc ggaatatggg ggacggtcat tgcggtttgc
     7261 tccaaaaagc ttcagacggc atatgccgtc tgaacaggga aaggggtcag gcgttggcgc
     7321 gttttttatc tttcaacaga aaaatcagca ccgccaatac aatgcctgtc gtgccaaagc
     7381 ccaacagcgc ggattttgtc agacccaatg cgaggtagcc cgatgcggcg gcggcggcaa
     7441 cggttaaggc gtaaggcagt tgcgaggtaa cgtggtcgat gtggttgcag cgcgctccgg
     7501 tggacgacag gatggtcgtg tcggaaatgg gcgagcagtg gtcgccgcat accgcccccg
     7561 ccattactgc ggacatacac gggataatca gcgcgggttc gactttgacc gccatggcgg
     7621 cggcaatcgg cagcataatg ccgaacgtcc cccagcttgt gcctgtggca aacgccatca
     7681 cgctggcgag caggaagagg atgacgggca ggaagccggg atggatgttg cccgcaacca
     7741 gtgtggagag gtaatcgccg gtgtgcattt cgccgacaac cgtactgatg agccaagcga
     7801 ggattaaaat ggcgattgcg ccgaacatag atttcgcacc ctgccaaacg gctttgggat
     7861 agtcggcggt tttaatcgtg ccgagcgtgc agagaacgac ggcaaggacg ccgcaagtgc
     7921 cgccgaatac cagcgaagtg tttacgtccg tgttttcaaa tgcccccaaa atgctgaagg
     7981 tttcgcttgc ctgcgcgccg gtgtagatca tggcggaaac cgttgaggcg attaaggcca
     8041 aaacgggaat aatcagtgcg taaacacgac ctttggtagc gtctgaaacg gcagtttcat
     8101 cgtgggcttc gttcaacgcg gcttgttcga aacgtgccat cgagccgatg tcgaaggaaa
     8161 accatgcgac gacgaacacc ataatcaggg caaacagtgc gtaatagttc atcaggctca
     8221 tggcgacaaa cgtccccatc ggcgtgtatt cggtgatttt gtaggtaacg agcagtccgg
     8281 caagcgtggc gataatcgac gcgccccagc ttgaaacggg catcagcacg cacataggcg
     8341 cggcagtgga gtcgaggatg taggcgagtt tggcgcggga aactttaaac ttgtcggtaa
     8401 cggggcgggc aatcgcaccg acggcgagac tgtggaaata gtcgtcgata aaggttacga
     8461 acacgaggca ggcggtcagc attttcgcgc cgcgccggtt tttaatgtgc cgttttgccc
     8521 agtcggcaaa cgcctgattg ctgccggagt aggtcagcag ggaagtaaaa atacccaaaa
     8581 gtatcaggaa aaccaagatt tttggtttgc ccagcgacca atcgccgtct gaccaagcca
     8641 agccgacgac catgtctttc aggtgtgtca gaccgtcgac ggggttgccg ccgaccaaaa
     8701 aggcaacgcc gaccagaata ccgatgccta aagacagcag tacgcggcgg gtaatgatgg
     8761 caagtgccag tgccaaaaag ggtggcacaa ccgagaaaaa tgaatgtgaa tagtcgatca
     8821 gctgcatggt tatgggggtg ttaagcgtcc ggatggggcg gtttctgtcc gcaacggttc
     8881 ggattttgtt ggcaaaatgg gcgaaagtat tttctgttcc ggaaaatact tatttgaaat
     8941 caattaactg attttttaaa atgcccgtta atcggtattg acgggcattt tatcatttaa
     9001 aaaatatttt ggttaaatta tgtgggttat tgcaggttta atgcgataaa cagcgtgttg
     9061 ccacggcgca tgaccagcag ggggacgttt ttgcctgcct tgtccatagc tttgcggaaa
     9121 ccggcttcgt cattgacggg gacttgcccg acggcgagga tttcgtcgcc gcgcctcaag
     9181 cctgcgcgtt ctgccgcgtc ggaaacccgt acgacgacga ggtgtccgcc gctgctgtcg
     9241 gtatgtgtct gaagggtaat gcctgcggat tcgaccgaga acgtaccgga ttgctgttcg
     9301 gtgtaggggg cttcatctgt tttggatgat gcgccgatat gctcggcggc gttgcccagc
     9361 ttgactttga ttgtgatttc ttcgcctttg cgccatacgc cgaggctgac ttcttttccc
     9421 ggcgtaatgg cgccgaccat aacgggaagg tcgccggaag aacgtatttc tccgccgtcg
     9481 aggctgagga cgatgtcgcc cgcctgcagg ccggcacgtt ctgcggggct gccgggcagg
     9541 attttggcaa tcagtgcgcc gccggctttg tccaaaccga acgattgtgc caaaccgtag
     9601 gatacttctt gaataatcac gcccagttgt ccgcgttgga ctttgccggt gtttttcagc
     9661 tgttcggtga cattcatggc aacgtcaatc gggatggcga aggaaatgcc catgaatccg
     9721 ccgctgcggc tgtatatttg cgagttgatg ccgacgacct gtccttttaa gttgaacagc
     9781 gggccgccgg agttgcccgg attgatggca acgtcggttt ggatgaaagg tgtgtagctt
     9841 tcgttgggca ggcttctgcc tttggcggac acgatgccgg cggtcacgct gttgtcgaag
     9901 ccgaagggcg cgccgatggc ggcgacccat tcgcccggtt tcaaatcttt gggattgccg
     9961 attttgacga cgggcagctc ttccgttgca tcgattttca gaagggcgac atcggattgg
    10021 acatccgagc cgatgagctt ggcggtatat tcgcgcttgt cgttgagcag gactttgata
    10081 ctgcccatgc cggtaacgac gtgcgtattg gtcagaatat agccgtcttt gctgatgatg
    10141 aagcccgaac cgaagttcaa tccgccgtca tctgcttctt cttgggggat ttcgggcatg
    10201 ttcgggacga ggcgtttgaa aaattcgtag aacgggtcgc tgtcggcaag cgggtcggaa
    10261 tcggtttcgg cattgccgct gccgttttgg gtgcgcgggg cgggggctgc ctgaatattg
    10321 acgactgccg ggccttcgct ttgaaccagt tggacaaagt cgggcagcag catactgacg
    10381 ctgccgtcgt ctttggtgtg ttcgatgcgt tctacgaagg atgcttcttt tttgtccgca
    10441 ccgaaaaagc tgccggcttt gtcgcagcct gccagcgagg cggcacacag tgctgccaaa
    10501 gcgaggtatt ggtatttttt gaacacgttt tgtcctttgt cggatgccgg taccggcttt
    10561 aatgccgtct gaagcgcatt ttgtcggctt cagacggcat aggttgaaat tctacaacgt
    10621 ccgtccgaat tttcaagcgt ttcattttga agggcggcgg cggtcaggct ttggcgggat
    10681 attcgcacaa atcgttgatg atgcaggttt ggcattgcgg tttgagcgcc ttgcaggtgt
    10741 agcgtccgtg caaaatcagc cagtggtgcg cgtccatcag aaattctttg gggatgaagc
    10801 gcatcagttt gtcttcgact tcgcgcacgt ctttccccgg cgcgattttg gttcggttgg
    10861 acactcggaa aatatgcgta tcgactgcca taacgggatg tccgaacgcc gtgttcaata
    10921 ccacgtttgc cgttttgcgc cccacgcccg gcaaggattc caaagcctcg cggtcttccg
    10981 gcacttcgcc cttgtatttt tccagcagga tgcggcaggt ctgcataatg tgtttggatt
    11041 tggttttata caacccgatg gttttcgtgt attccatcac gccgtccaaa cccaaatcca
    11101 gcatcgcttg cggcgtatcg gcaatcggaa acagcttcgc cgtcgccttg tttacgccga
    11161 catcggtcgc ctgcgccgaa agcagaacgg cgattaaaag ctcgaaaggg gagttgaaat
    11221 tcagctcggt ggtcggatgg gggttggcgg cgcggaagcg ttcgaaaatt tcttggcgga
    11281 tgtgtctgtt catttttttg tgcagtcggt ttgtgcggtc ggcattataa cgcacggttc
    11341 aggcggcgta atattgcatt ccccacagaa tgaaggcgta acgcgccgtt ttgccgataa
    11401 ccagcatcag cccgcttgtc cacggattca accgcagcca gccggcggca agcggcagtg
    11461 cgtcgccgac gacgggcagc caggcaaacg gaagcagcca aataccgaaa cgccgcatca
    11521 gattcagtgt tttttcagac ggcatttttc gggaaggcag caaacgtccc atccaatagg
    11581 aaaccatact gcccaatccg ttggcaaggc cggcgcacag caacgcgccg tatgcgtgtt
    11641 cgggaaagcg gcggacgaac agggcaaagg cggcttcgga tgtgccgggc aggagggtgg
    11701 cggaagtgaa ggcggaaaag gcgagggcgg cgtaggtgta ggagggtatc attgcaaaca
    11761 gtctcaaaca ggtaacaatc ggcgacggat tg
//
LOCUS       DAWKQJ010000049        11507 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000049 DAWKQJ010000000
VERSION     DAWKQJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.049"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            122..544
                     /locus_tag="WJL20_001005"
     CDS             122..544
                     /locus_tag="WJL20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683170.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            583..1323
                     /locus_tag="WJL20_001006"
     CDS             583..1323
                     /locus_tag="WJL20_001006"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ7683171.1"
                     /translation="MLIVRPSGRAKDDVETCRRAEWQAEVLSPIEIEADAAGLELLSE
                     QYARADAVFWVSPTAVETAVPYLNLSDGIKIHIAVGQGSRRALERYLGRTVIAPDDGN
                     DSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFRRHKPLNF
                     QNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1338..2672
                     /locus_tag="WJL20_001007"
     CDS             1338..2672
                     /locus_tag="WJL20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7683172.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2669..3886
                     /locus_tag="WJL20_001008"
     CDS             2669..3886
                     /locus_tag="WJL20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ7683173.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYHRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3961..5025
                     /locus_tag="WJL20_001009"
     CDS             3961..5025
                     /locus_tag="WJL20_001009"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ7683174.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGSQVALQGNFDPFALFGTPESIRTEVARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5203..6582
                     /gene="radA"
                     /locus_tag="WJL20_001010"
     CDS             5203..6582
                     /gene="radA"
                     /locus_tag="WJL20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ7683175.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6857..7336)
                     /locus_tag="WJL20_001011"
     CDS             complement(6857..7336)
                     /locus_tag="WJL20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ7683176.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(7468..7827)
                     /locus_tag="WJL20_001012"
     CDS             complement(7468..7827)
                     /locus_tag="WJL20_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ7683177.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7866..11480)
                     /gene="recB"
                     /locus_tag="WJL20_001013"
     CDS             complement(7866..11480)
                     /gene="recB"
                     /locus_tag="WJL20_001013"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ7683178.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSNDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGITMLKRNWQRVTDNAPADTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2482 a         3031 c         3433 g         2561 t
ORIGIN      
        1 gtgccgccct atgtgttgcc ctaatttgga aggggttaca cccttttcaa ataaaatctg
       61 atgctgctgc cacgaaggac ggatgtccaa gtggcggggt tttaaccatt aaggaaatac
      121 gatgaaaaaa acgttccttt ctgccgtatt gcttctgtca gctgcgaccc aaaccgtgtg
      181 ggcagatacg gtgttttcct gtaaaacgga caacaacaaa tatatagaag ttcaaaaaat
      241 taaccgcaat ctttacgaat attcgttcgg cagtgcggca aaaaaagaaa ttgccatacg
      301 caacagcaaa gctgacctgt tggggcgttc cgacaggtgg caaggctggg gcagaggccg
      361 ttgggcgacg atgaaattcc aaaacggcga atttatgtac accgtagatg caggcttcga
      421 ttccgtgacc catgcagtag gcagcggtgt ccttgtggag cgtaggggca aggaaatcgc
      481 acggttcgac tgtacgtcga aaaccgcgca ggcgaatttc gacgatgacg attttgcctg
      541 gtaatcgggg cggataaggc gatagaaaca gtgaaaccca tcatgctgat tgtccgaccg
      601 tcgggcaggg cgaaggacga tgtggaaacc tgccgtcgtg ccgaatggca ggcggaagtg
      661 ttgagtccga ttgaaatcga agcagatgct gccggactgg aacttttgtc cgaacaatat
      721 gcccgtgcgg atgccgtgtt ttgggtcagt ccgaccgccg ttgaaaccgc cgtcccgtac
      781 cttaaccttt cagacggcat aaagatacac attgccgtag ggcagggcag ccgccgcgca
      841 ttggaacgct atttgggcag aacggtcatt gcgcctgatg acggcaacga cagcgaggcg
      901 gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg cacgcgtatt gtttgtgcgc
      961 ggacacggcg ggcgggattt tctgatgaat gccttgcagg agaaaggttt tcggacggag
     1021 atggcagaag tctatttcag acggcataaa cctttgaact ttcaaaattt ccaaaccgaa
     1081 aatattgccg ccgcctatat tacgtcgacc gagctggtgc gcttgctgtt cgggcagctt
     1141 ccgccgcaat tttcccgatt cttcaaatcc ttgctatact ttacccatca tccgcgcatt
     1201 gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa ccgtccctac gctggaagcc
     1261 gcgctttccc attcttccat ttccgtttca gacggcatgg tctttcccgg aacctcaaat
     1321 taataaggag caaaacggtg ggcgaacctg aaaacaaatc atccgaaccc gtacgcgaga
     1381 tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc acgcaaagaa aacgaaacag
     1441 aagtacacat tcctgccgct ccttttatcg tcaaacagtc cggcagcaac gctttggcag
     1501 tctgcgccct ggtattggcg gcattgggtt tgggtacaag tggttttttg tttgtccaag
     1561 gacagaatgt cttgaaaaac caagagctgg cattcaacca aaaaatcgac aaagccgcct
     1621 tgggcgagtc ggaaaacgcc gccctgttga aagacaacct caaccggcaa gccgccatac
     1681 aatcagagct cgaccgtttg gacggaaacg tcaaagcaaa cggcgaacaa atcttggaaa
     1741 tgcaaaaatc ctatcgcgag ttgaccaaag gacgcgccga ttggctggtg gacgaaaccg
     1801 agaccatact caatctggcg gcgcaacagc tggtgttgac tggcaatatc caaacggcag
     1861 tcggcgtatt ggagcatatc gacagccgcc tgtcccgttt caatcaggca gagcttctgc
     1921 cgatcaagca ggcggtcagc agcgacttgg cggaactgaa aaaccgtccc tatgtcgata
     1981 tttccggcac ggcattgcgc ctcgacaggc tggaaaccgc cgtatccgga ctgccgctga
     2041 tgctcgacgg cgtattgaaa ccgggcgtac aggtgaagaa cgaagccgct tctgcttcat
     2101 ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa ggggctggtc gaaatccgcc
     2161 gtttggaaaa caacgatgcc atgctgattt ctcccgaaca ggcatatttt gtgcgtgaaa
     2221 acctgcgcct ccgccttttg gatgcgcgga ctgcattaat gcagcgcaac agcgaagtct
     2281 atcagggcga tttgaacaat gccgaagccg ccgtcagaca gtatttcgat gccaagtctc
     2341 ccgccacgca gtcgtggctg aaagaactgg cggaattgaa ggcgttggat gtgcgcatga
     2401 cggcggatga cggtttgaaa aacagcctaa atgccgtccg cgcctatcgc gacggtacgc
     2461 gcatgacggc ggcggaaaat caagaagcag aacaggcggc ttccgaaccg gcaaacgaaa
     2521 acacagcttc cgaaccggct gccgcatcgg atgtgaagac catagaagca ccgtccctgc
     2581 cttcggaacg caaaccggaa cagcctgcaa aaaaacagcc cgtaccggaa aaggcagggc
     2641 gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta gtctggattg tcgtcctgtt
     2701 tgccgccgcc gtcgggctgg cattggcgtc gggcatttac accggcgacg tgtatatcgt
     2761 actcggacag accatgctca gaatcaacct gcacgccttt gtgttaggtt cgctgattgc
     2821 cgtcgtggtg tggtatttcc tgttcaaatt catcatcggc gtactcaata tccccgaaaa
     2881 gatgcagcgt ttcggttcgg cgcgtaaagg ccgcaaggcc gcgcttgctt tgaacaaggc
     2941 gggtttggcg tattttgaag ggcgttttga aaaggcggaa cttgaagcct cgcgcgtatt
     3001 gggaaacaaa gaggcggggg ataaccggac tttggcattg atgttgggcg cacatgccgc
     3061 cgggcagatg gaaaacatcg agctgcgcga ccgttatctt gcggaaatcg ccaaactgcc
     3121 ggaaaagcag cagctttccc gttatctttt gttggcggaa tcggcgttga accggcgcga
     3181 ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag atgaatgcca accttacgcg
     3241 cctcgtgcgt ctgcaacttc gttacgcttt cgacaggggc gacgcgttgc aggttctggc
     3301 aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa tcggaaatgg aacggtatca
     3361 aaattgggca taccaccgcc agctggcgga tgctgccgat gccgccgctt tgaaaacctg
     3421 cctgaagcgg attcccgaca gcctcaaaaa cggggaattg agcgtatcgg ttgcggaaaa
     3481 gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg gtcaaacagc attatccgca
     3541 caaccgccga cccgaacttt tggaagcctt tgtcgaaagc gtgcgctttt tgggcgaacg
     3601 cgatcagcag aaagccatcg attttgccga tgcttggctg aaagaacagc ccgataatgc
     3661 gcttctgctg atgtatctcg gtcggctcgc ctacggccgc aaactttggg gcaaggcaaa
     3721 aggctacctt gaagcgagca ttgcattaaa gccgagtatt tccgcgcgtt tggttctggc
     3781 aaaggttttt gacgaaaccg gagaaccgca gaaggcggag gcgcagcgca acttggtttt
     3841 ggcaagcgtt gccgaggaaa accgaccttc cgccgaaacc cattgaaacc ttcagacggc
     3901 atcgttgaaa tgccgtctga aaaccatttc acttgacaaa cagactgaca gaaaacccgt
     3961 atgactcttt taaaaaacga taccttcctc cgcgccctgc ttaaacagcc cgtcgaatac
     4021 acgccgattt ggatgatgcg tcaggcgggg cgttatctgc ccgaatacaa agccacacgc
     4081 gcgaaagcgg gcagcttcct cgatttgtgt aaaaacaccg aattggcgac cgaagttacc
     4141 atccagccct tagaacgctt cgatttggac gcggcgattc tgttttccga catcctgacc
     4201 gtccccgacg caatgggctt gggactgtat tttgccgaag gcgaaggccc gaaattcaaa
     4261 cgcgccatgc aacacgaggc cgacatcgcc aagctgcacg ttcccgatat ggaaaaactg
     4321 caatacgttt tcgacgcggt aacttccatc cgtaaagcat tggacggccg cgtaccgctc
     4381 atcggcttct ccggcagtcc gttcacgctc gcctgttata tggtcgaagg cggcggcagc
     4441 aaagaattcc gcaccatcaa aaccatgatg tactcgcgcc ccgatttgct gcacaaaatc
     4501 ctcgatacca acgcccaagc cgttaccgcc tacctcaacg cccaaatcga cgcgggcgcg
     4561 caggcggtgc agattttcga cacttggggc ggcgtgttga gcgatgcggc gtttaaagaa
     4621 ttcagcctca aatacatccg ccagatcgtc gccggactca aacgcgaaag cgaaggccgc
     4681 cgcgtgcctg ttatcgtatt tgccaaaggc ggcgggctgt ggctggaaag tatggcccaa
     4741 atcggcgcag acgcattggg cttggactgg acgtgcaaca tcggcgaagc acgccgccgc
     4801 gtcggcagcc aagttgccct gcaaggtaac ttcgacccgt tcgccctctt cggtacgccg
     4861 gaatccatcc gcaccgaagt cgcgcgcatc cttgctgact acggacaagg cagcggccat
     4921 gtcttcaacc tcggacacgg catcaaccaa cacgccgacc ccgaacacgc caaaatctta
     4981 gtcgataccg tacacgagct gtctcggcag tatcacggcg ggtaagccgg caggaaaccg
     5041 cccgatatgc cgtctgaaag ccgagggatg gaaaattagg taatacggca atatccgccg
     5101 tgtacggata gtacatgacg gcggcggtgt tgccgcattg gcgcaatccc aaccgtccct
     5161 attttcagac ggcatttttg ttttcagatg cagggaaaac cgatggcaaa aacgcttaaa
     5221 accctttacc aatgcaccga atgcggcggc acttcgccga aatggcaggg caaatgcccg
     5281 cattgcggcg agtggaacac gcttcaggaa agccttgccg cgcccgagcc gaaaaacgcc
     5341 cgcttccaat cttgggcggc ggatacctcg accgtccaat ccctctcctc tgttaccgcg
     5401 accgaagtgc cgcgcaatcc gaccggtatg ggcgaactcg accgcgtatt gggcggcggt
     5461 ttggtcgatg gtgcggtcat cctgctcggc ggcgatcccg gcatcggcaa atccacgctg
     5521 ctgttgcaaa ccattgccaa aatggcgcaa agccgcaaag tgctgtacgt ttccggcgag
     5581 gaatctgccc aacaggtcgc cctgcgcgcg caacgtttgg aactgcccac agaaggtgtg
     5641 aacctgcttg ccgaaatccg catggaggcg attcagacgg ccttgaaaca gcatcagccc
     5701 gaggtcgtcg tcatcgactc catccaaacc atgtattccg accaaatcac ttccgcccca
     5761 ggctccgtgt cgcaggtgcg cgagtgtgcc gcccaactga cgcgcatggc gaaacagatg
     5821 ggcatcgcca tgatactggt cggacacgtg accaaagacg gcgcgattgc cggcccgcgc
     5881 gtgctggaac acatggttga taccgtgctg tatttcgagg gcgaccaaca ttccaactac
     5941 cgcatgatac gcgccatcaa aaaccgcttc ggcgcggcaa acgaactggg cgtgttcgcg
     6001 atgacggaaa acggtttgaa aggtgtgtcc aacccgtccg ccatcttcct cgccagctac
     6061 cgcgacgata cgcccggctc gtgcgttttg gttacacagg aaggcagccg cccgcttttg
     6121 gtcgaaattc aggcattggt cgatgacgcg cacggcttca cgcccaaacg cctcaccgtc
     6181 ggactggaac aaaaccgtct tgcgatgctg cttgccgtgt taaaccgcca cggcggcatc
     6241 gcctgtttcg atcaggatgt gttcctcaac gccgtcggcg gcgtgaaaat cggcgaaccg
     6301 gcggcggatt tggcggtcat cctcgcgatg ctttccagct tccgcaaccg ccctatgcct
     6361 gaaaaaaccg tggttttcgg cgaaatcggc ttaagcggcg aagtccgccc cgtcgcacgc
     6421 gggcaagagc ggctcaaaga agcggaaaaa ctcggcttca aacgcgccat cgttcccaaa
     6481 gccaatatgc cgcgcaacgc caaagagttt ccgaacctga aaatctacgg cgtttcgagt
     6541 ttgcaggaag ccatcgatat ttgccgtgac agcagggaat aaacggaaat gccgtctgaa
     6601 atcaggtttc agacggcatt tggtttgtgg cggattgaaa caagaaggca taccggcgac
     6661 agataagatt tgcggcaaag ttgcctgtga tatggcaaaa cgcacacgcc cgtcatcccc
     6721 gcaagggcgg gaatccggaa tcgtccgttt cggcaatgat tgaaaatcac ggtaacccaa
     6781 ccgcttggat tcccgacttc gtgggaatga ggggcgtgtg tgtttgattt ccatccgcca
     6841 tatgtcggcg acgggcttat tcgcctacgg ttttttgtat cagtttttcg gcgtttgcca
     6901 aagtgtttgc cacttcgtca aaaccgatgc ggctgccggc gatgagggcg cgcgtatcgg
     6961 tataggcggc gcgtactttg ccgtctgttt cggtaacgag gacgcgcagg ggcagttgca
     7021 gggcgaaggc ggggtctttg accatcagcg gcgtgccggc tttgggcgtg ccgaagacga
     7081 tgacttttgc cggctgcatc gttaagccgt ttcggtgggc ggcttcctga tggtcgatga
     7141 cggcaaaaat gtccatccct ttgcttttta tggcggtttc aaggcggctg acggtttcgt
     7201 caaaactgta ttttgaggtg agggtatgcg tggtcatagc ggtttcgttt tgggtttgcg
     7261 gttcgctggc aggatgtgcc gaagcggttg aaatgcagag tgcggatgcg gcaatcaggg
     7321 ggagtatgtg tttcatcgta tttccttttt ccttttcggt ttgtttatca ggcttcggac
     7381 ggggcggcac gcgccgcgcc cgatgttgcg ccgcgtgctg gggtgtcgta tgccgtctga
     7441 aagcctgtcc tttcagacgg cattgcgtca tttcatccct tttttgagca ggtcttcata
     7501 accgccgtga ttggcaacat ttgtataacc tgcttttttc agctcctgaa gggcggcttt
     7561 ggcacgccgt ccgctgcggc agtagaggtt gaccggcgtg tctttgtcgg gcgcggcttc
     7621 gtgtatgcgg cggacgattt ggtcgacggg gatgttgacc gcgttgtgca aatgaccttc
     7681 gctaaattcc tgttcggaac ggacatcgat ccaaacggcc ggatgttgcg cggtttgggc
     7741 ggcggatagg ggtttttgcg gggctgcctg cgcggcaaag gcggctgagg caatgagtgc
     7801 ggcggtaatc aggtgtttga tattcatagg gttctcctgc ggttgtcgtc ttaagggacg
     7861 ggaagttatt ttatctgttc caaagcggcg gcatctatgt cccaacgcca aacgccgccg
     7921 cctttgccat ccaatccgcg caaaaacagg tagcggacgg aaacggcggc gggcggttgt
     7981 ccgcgcagtt tgaagtagcg tgcggcggca acggcgtaaa tcagcgcctg aaggtaatag
     8041 tggtggtgcg cgacggcttc gtccatcgcc tgttgcgtgt aggcggatgc gtccgcgccg
     8101 agatggtttg atttgtagtc gatgacgcag atattgccgt cggggtcttg gcagaccata
     8161 tcgataaagc cgtttaaaaa gccgttgacg gtgtggaagt cgagcgtttc ggcggcggca
     8221 cggcagactt cgggcaatcg gatatcgtcg cgggcaaacc agtcgcgcag ccgtttgagg
     8281 ccgaagtctt cggtgtggag ggtaaagccc atttcgggac agcggcactc gggcggaatg
     8341 tcggacaggc agtatgcccc cgtcagcggc gttttacggc aggcttccgc catttcggca
     8401 acggcgggca gccacatttc ttcaaacccg tatttcttca gcttgtcggc aatgagggtt
     8461 tcctgtccgg cggctgcttg tccgaatttg aaatcttcaa gaatttcgtg caggcacagc
     8521 cccgcctgcg tgcctttcgg aaaatcgtgt atcgatatgc cgtctgaagc cgtcggcgtt
     8581 tcagacggca tcgccggcac cgaggtttcg gcggcatcca aggacgggca ggcatcttct
     8641 tcgccgccgt cgggcgtttg ggtatggcgg cttaaagcgg taaagctggt gtggcggaca
     8701 aatcggaatc tgcgttcggg aatgctgttt gcggcaaatt cggcggtttg accggcgttg
     8761 ccgcgatagg cggcaggggg cggcgcatct tctgtgaagg cgaaatccgt gtcggccggg
     8821 gcgttgtccg tcacgcgctg ccagttgcgt ttgagcatcg ttatgccgtc tttttcacac
     8881 gcataggcac ggcggacggt ttcacggctg tcttgcggca agccttcaat caggtaggcg
     8941 agggggttgt cggcggtatc ggaagaatag gcggcgtaga tgttgagctg ttcctcggca
     9001 cgcgtcagcg cgacataaag caggcgcagg cgttccgcca tttcttcgtc ggcgtattgc
     9061 ttctgttcgt cttccgacag ttgcgccttt gccaacagtt cggttcggtt ggcgctttgg
     9121 tggaggattt gccagtcgga cggcccggta tcttgcgcgt cccacgcaaa cgggcagtac
     9181 accagcggat actgcaaacc tttcgaggcg tgcatggtaa cgattttgac caaatcttcg
     9241 tcgctttcca gacggatggc gcggttgtcg ttgctgttgt tttcggcaag gctgatttgg
     9301 tcgcccagcc acttgtgcaa agcggcgggg ttgcggtttt gggcgtcttc ggcggcaagc
     9361 agttcgagca gttggaaata attggtcagg ctgcgcccgt tgttccggct taagaggcgc
     9421 gtttcgatgc cgtgtgtttg ggaaaattgc tgcatggcgg cgaaaatgcc gtattgattc
     9481 cagttgtcga gcgcggttcg ggcagattcc gcccaatgca aaatctcgct ttcgttttgg
     9541 ttgaagtcgt gcaattgctg cgcgtcataa ccgaatatgc cgcttgtcag gacaaagcgc
     9601 agcgttccgg cgcggcgcgg ttcgagccag aagccgatga gtgcggaaag ggcggcggct
     9661 tcgggcgagg cgaacacgga ttcgcgcgaa agcaggacgc tttgcacctg ccgttttttc
     9721 agggcggcgg aaaccatcac cgcctcgttg tgcgtgcgta ccagcacggc aatatcgccc
     9781 gactgcaacg ggcagccttt gaaattcaga cggcctccgg cggcttcgtt gagcgcgtgg
     9841 gcgatttcgt cggcgcaata gtcggcggca cgtcggcgca aaacgtcttt gttggctttt
     9901 tcattgtcgt tttcgtgcag ccaacgaacc tgtacggcag gacgtttggg cgacaatctg
     9961 ctttcggcac gcgccgcacc gacttccgaa tagccgatgt tttccaaaac gaacgggcgt
    10021 tctttgaggc ggaacagcgc gcctatgctg ccgataagcg cggcgtggct gcggtagttg
    10081 gtggcgagcg tgtagcggtg ccgcgcgtct tccgccgcct gaaggtaggc gtaaatgtcc
    10141 gctccgcgaa agctgtaaat cgcctgtttg ggatcgccga cgaggaacag cggtcggttt
    10201 tgggcgatga agattttttg gaagatttcg tattgcagcg ggtcggtgtc ttggaactcg
    10261 tcgatcagcg cggtttccca gttttcggca acggcgcggg cgagggtttc ggcgtgcgga
    10321 ttgtcggtca gcgcggtgtg gacatcgagc agcaggtcgt cgaaaccgcg ttcgcggcgc
    10381 gattttttca tctcggcaag gctgcggttg aggtattcga ttaaatccag ttgcagccgg
    10441 atcattgttg cttcttccgc ttcttcgagt gcgttcaaat cgcgcccgaa gtctgccagt
    10501 ttctgcaatt cggcaaatac tgccgcatcg ggcgttttgc cttttttcag tccggcttcg
    10561 agtttgtcgg atgcaagttt caagagtctg tcgtgtgtgt ctttgtccag aaagggcagt
    10621 tgtccggcgg cggatttttg tgccagttct ttaaaaaggt tgccgaagct gtttttgcgg
    10681 taactgttgc cgttgaggtc gggatgaatg cgccaaaagc cggcttccag ttctggcagc
    10741 aggcggcaga tggtttgcca tgaggtttcg gcgttgcgct gcgcctgttt caaatccgcc
    10801 tgcggacggc ggaaattcag gtacgggcgg gaaagatagc gcgaaatttg ggcaaggacg
    10861 gtttgcggca cagctttgcg tttaagcgcc aatgcggcaa gcaccggatc attgctgacg
    10921 cgttcccgcc aaaaatcttg cgccgggata agcaggcggt cgccgtcttc ttcggtcatt
    10981 tcgacatcga acggtgcttg gcacaggaag gcgtagtcgc gcaggatgcg ctggcagaag
    11041 ccgtggatgg tatagatggc ggcgttgtcg aattgcccga tggcggcctt gaggcggaca
    11101 atcagacgcg tccggccctc tttttgcaaa gcctgtttta agagttcggg caggaaggtg
    11161 tcgccttcgt ggtgttcggc gcagtaggcg gcaatgccgt ctgaaagcgt gtcgtctcca
    11221 agtttggcaa ttcctttgct ttctaaaact tgtaacacat cgtccaaacg cccgcgcagg
    11281 cgtgttttca gctcggcggt ggcggctttg gtgaaggtta cgaccaatac gcgttcgacg
    11341 tttttttgtt ctaatacgat caggcgtgta aacagggcgg caatgccgta ggttttgccg
    11401 gtgccggcag aggcctcaat caggttggtg ccggaaatgg ggacggttag cgggtcgaat
    11461 gcttggatgc ttgcagacat agtgcgcgct cggaaaacgg ttggacg
//
LOCUS       DAWKQJ010000050        11485 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000050 DAWKQJ010000000
VERSION     DAWKQJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.050"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            143..310
                     /locus_tag="WJL20_001014"
     CDS             143..310
                     /locus_tag="WJL20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683179.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYTQNIDFSQW
                     VKSIIKEKIKK"
     gene            583..768
                     /locus_tag="WJL20_001015"
     CDS             583..768
                     /locus_tag="WJL20_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683180.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            969..1169
                     /locus_tag="WJL20_001016"
     CDS             969..1169
                     /locus_tag="WJL20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683181.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            1301..1909
                     /locus_tag="WJL20_001017"
     CDS             1301..1909
                     /locus_tag="WJL20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ7683182.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            1913..2128
                     /locus_tag="WJL20_001018"
     CDS             1913..2128
                     /locus_tag="WJL20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683183.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(2289..2807)
                     /locus_tag="WJL20_001019"
     CDS             complement(2289..2807)
                     /locus_tag="WJL20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ7683184.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(2917..3084)
                     /locus_tag="WJL20_001020"
     CDS             complement(2917..3084)
                     /locus_tag="WJL20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683185.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(3077..3250)
                     /locus_tag="WJL20_001021"
     CDS             complement(3077..3250)
                     /locus_tag="WJL20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683186.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(3359..3625)
                     /locus_tag="WJL20_001022"
     CDS             complement(3359..3625)
                     /locus_tag="WJL20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683187.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(3715..4629)
                     /locus_tag="WJL20_001023"
     CDS             complement(3715..4629)
                     /locus_tag="WJL20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ7683188.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            complement(4665..6641)
                     /locus_tag="WJL20_001024"
     CDS             complement(4665..6641)
                     /locus_tag="WJL20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7683189.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYEMFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVAKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     EELATMFMPQEVRKVQRGWLDLFNNSYFSTELVEYHKDEVRVSYDLDDASAVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            complement(6643..6894)
                     /locus_tag="WJL20_001025"
     CDS             complement(6643..6894)
                     /locus_tag="WJL20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683190.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            6942..7841
                     /locus_tag="WJL20_001026"
     CDS             6942..7841
                     /locus_tag="WJL20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ7683191.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            8003..8215
                     /locus_tag="WJL20_001027"
     CDS             8003..8215
                     /locus_tag="WJL20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683192.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            8437..9015
                     /locus_tag="WJL20_001028"
     CDS             8437..9015
                     /locus_tag="WJL20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ7683193.1"
                     /translation="MFRHNGEVLLDGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYL
                     IDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMHLDVGNVACG"
     gene            9120..9788
                     /gene="hda"
                     /locus_tag="WJL20_001029"
     CDS             9120..9788
                     /gene="hda"
                     /locus_tag="WJL20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ7683194.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            9785..10453
                     /locus_tag="WJL20_001030"
     CDS             9785..10453
                     /locus_tag="WJL20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7683195.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            10809..10916
                     /locus_tag="WJL20_001031"
                     /pseudo
     CDS             10809..10916
                     /locus_tag="WJL20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3108 a         3054 c         2326 g         2997 t
ORIGIN      
        1 actattcaaa ttctcatctt ggtcgcagtt ttcatgttgg caagtgctaa tgcctactaa
       61 agactgacca gctgggggcg gtcgccgctg tccccagtcc caatcttaca aaatacggtt
      121 taaaaaatca aggagtttgc ctatggttga tgaaaaactg gcagaaaacc gaaagcgtta
      181 cgagcagaaa cgggtgatta agaaagtctc gttcaatgcc gaaacggaaa aagaactttt
      241 ggaatatacc caaaatatcg atttttccca atgggttaaa tcaatcatca aagaaaaaat
      301 caaaaaatag cttgactaaa ttctagaaat actagataat acgcctatct gataaagcaa
      361 agccccgaag cggcaacttc gggggcttat cggatagaaa ggaggcttgt ggaaatgaca
      421 aaactggcga aagttgtttt tttcatcatt ctgcttttaa gctgtagtcc ggcttactaa
      481 gcaagttgat taaacacaag gggtggcaga acaccgcgcc cctttcgcca acctttctgc
      541 aagccatttt actacaacga ttttaaaaat caaggattgc ctatgcctga tgaaaaaacg
      601 ctaaaagcaa aacaagctga atatcagcga aagtatgacc aaaagcggct gatgaaaaca
      661 gtttctttcc atttggaaag agagaaagag ttggtggatt ttatgcaaaa agaaattccc
      721 gacttttcaa actgggttaa atcagtcatg aaggaaaaaa tgaaataaga aaattccgcc
      781 gatgggaaaa catcggcgga acaggtggaa agcagagcag caactctctt tatgtagggc
      841 atgaaaaaag gccgtctgaa acaggtttta aaccccattt cagacggctt tttgttgtaa
      901 tacatcttga ttaaaatggt ttttagtatt accataatcg tgttttaaaa cctatttaaa
      961 ggaagattat ggactcgatt aaaaaggctc aagaaaaata cgcactcaag cgagtagtga
     1021 aaacagtatc gttcaattcc gaaaaagaac ttgatttaat taaattttct gaaaatttag
     1081 atttttcaaa ctgggtaaaa tcgaaaatta gaggagatat ggaatcggaa aaattcaaaa
     1141 aaaaagcaaa aaagaatatt gacaaataga attaccataa tttaaaattt ccatatcttg
     1201 tgcaacacaa gatatggaaa tccccgataa gtgacagctt atcggggata gtggaaaagc
     1261 aacgttagag cgttcttttc cttgtcatct caaccttgaa atgaaagata attttaccat
     1321 gaatacccaa ctcatcccaa ctgtttcagg tcaattggac aatcaaacac aagcactggt
     1381 tgatgctcat gacctgcata agttcttagg tgtggaaaca ccattttcta aatggattca
     1441 gcgtcgcatt gaagaatacg gatttacaca agcccttgat tttatcggcg tggacaaaat
     1501 tgtccgcacc gaagcaggtt tctttggaca gcgtgataaa actgttcagg ggtattattt
     1561 atccctcgac atggcaaaag agctgtgcat ggtggagcgc aacgacaaag gacgccaagc
     1621 ccgccgatat tttatcgaaa tggagaaaca ggcaaaagcc ctgcctgatg ccgtgctata
     1681 ccgcatcgat gcattggaag acgcctattt ccaagccgcg cccgaaatgc tcgcgctgct
     1741 gcgctaccgc agtatgggct tgaacctgac cgaaataggc aaactcttgg acatgaaccc
     1801 cggcgcggtt tcctaccgtc tcaaaaaact caacgatttg ggtttcttgg aatatgtccc
     1861 caagcccaaa atggtggtgc aacaacagtc tttgggattg gagggttaag ccatgaatac
     1921 cacctatacc ctgactttcg accaagacag cctcaaagcc ctgaatctgc tggtgttcac
     1981 actcaaccat ctgaaagttt tggagatgga catggaagga atcgaagacg gtttgtctgc
     2041 cgtgattgaa accgcctccg agaaagccga caagctgtcc gccgctttct acaacgccgt
     2101 gtacgagcag gaagagaaag cagcctgaat ctagggcatg aaaaaaggcc gtctgaaaca
     2161 ggttttaaac cccgtttcag acgaccttta aatcatgggt taaagcctat ttgaaccggc
     2221 taggcaagct cctgctccgt aggctcaatc acgtttcaac acactgaatt gaaatcaggc
     2281 tgttgatttt atagaatttg ttcgctcggc tcaatcacaa aatcctccac gccgctcaca
     2341 atcttaatcc ccggcacttg gccgtctgaa aaacgctctt tttgattcag gatggcgtct
     2401 ttgtcgattt cctttttagt gcggacaaac tcggcaaagg cggatttttc cgagagccac
     2461 gccaagacgg cggccacgcc cgttaccttg acggatggcg gacggatgcg ccatttaatc
     2521 aggccggtag taaagtccac ggttttggtt ttaccgtttt ccgtcagctc gtccttatgt
     2581 gcctcgcagt atgcggccac acgttcggtc aggctcatga tttcggcaca catcggcgcg
     2641 gcttgggcgg catattcttc ttcgatggcc gcttttttgt ctccggcttc ggtttccagg
     2701 cgtttgactt cgcgctgcaa gtcgccgatt tggcggataa atgcagtaac ttccgttttg
     2761 tcttgtgccg cttcgatggc gggctgtttg attcgggttt tagccatttt ctcttccttt
     2821 caggttgggt ttaacatttc ggcttactca agccttgttc gcgttcgtgc ttggcaagtg
     2881 cttcaacttt cgcgcggttt ctcaaaaact taaacatcac acttcctccc ataaagttat
     2941 cgcctgagcc aaagtttccg cctcggcagt cttccacatc ccgtccggcg cacgcgctgc
     3001 aatcacaaag ccttcgccgt ccttcttcat gaccatcagc tcgccacggt cttcgagcca
     3061 ttcgattaaa tctttttcat tcatctttgt ttccttccgt tttcagacga cctttgccgt
     3121 ccttatcttc aaactgcgcc tgatattcgg cgacttcctg ctcgcggttt cgcttcacca
     3181 taaacttcgt cgcacgcctg cggtgttgtc cccaagcctg ccaatccgta ttacgtcgtc
     3241 cgaaactcat ttcacacatc ctttcacaat cgccttatcg ccatatttcg cgcggatttc
     3301 ctttaccgcc cgtgccaaag cctctttttt cgccgcaggg ctcaataaaa ttggcttatc
     3361 actcataaac aatccctttc attttttcct cgacgctcat tgactcgtat tgcccttccc
     3421 ataccttgac ctccaaatcc gcctgtcttt ccatcgcctc aaccttcgtc ggctctttcg
     3481 caaccggttc gggttctttc gcgcaggaat gcaacgccat tcccgccaca aaacaccaca
     3541 cgcccaccgt caaaccaacc ggcacccaac gccaaaaaga gcgcgccaca aacatcttcc
     3601 aatcaacttt cttcaaaact tgcattttgc gttttccttt taaaacaaca acttattaaa
     3661 attatagggt aaaaaaatat atagccctgt cagagactta ccgtttcaga cggcctatcg
     3721 gataatcagg gagctgtatt tcttaacgat accggcctgc atcttgatac cgttcttgtt
     3781 cgccgtgcgt accgcgccgc gcatcaattt actcatccgg cgcgtattgc cgttactatg
     3841 tttaaccagt tccaagagcg tttcttcgtc cgcatcaggc aaagccgctt tcgcaatctc
     3901 aaagagttcg tcatccggca aagattcgcc caaattcagc gcaacagaca cgcggctata
     3961 aagttgcacc agctcgccat gcttaccgcg caaattcgca accagtcggg gcataccgct
     4021 caacaccaaa ccgcagcccg tttcatcatg cagtcggcgg ataatctcaa gggcgcgcaa
     4081 gggcaggttt tccgcttcat cgaccacaat cagacggccc gaatcgcgca ggcggtcaga
     4141 tacagactca aacaaatcat tcaggctgcc catcgccgat accttcgccg ccgtcgccaa
     4201 cttgcgcatc agtaccaaag ccgtaaagct cggattagcc tcaatcaaga tagccgcagg
     4261 gtttttctcg cagtagtttt tgaccgactg cgtcttgccc aaacccgcct gaccgtagat
     4321 caccaccgtt tcgccgcctt cgtgcgcatc gcgcatcact tccgcgattc ggcgtgtcgt
     4381 cttagtcgat acaaatccca acaccagctc ttcacgtcgc gccttactgt cctccatctc
     4441 caaaaacgcc tcgattttcg tctcgatggt ttcataattg ccgcctttat cggcataagt
     4501 gccgttcaga tacatactga tggaggccgg cgaagtatcg ataccgcgtg ccagttgggt
     4561 ttggttcatt cccgattttt ctttaaattc aaccagtttt tgttgcaatg cttgattaat
     4621 ttgtttcatt tttttagtcc ttttaaaaga ggtttaaaac cgttttaact tctatccgtc
     4681 tcaaatagca caaaatcgtc cgtgcccgtt ttcggtaata ccgcatactc cgcctcgatg
     4741 acgtttccgc ccaaatgtcc cagctcgtcc caaaccgcag cctgttccaa agccggattg
     4801 acttccgcat ttgcgagctt gattgcattt tccgcccgct tgattttgcc ttttcggcgt
     4861 ttttccgcca gttggtcgat acgagccgtc gggaaagcct cgcgggtatt gccgtcggcc
     4921 tgagccttag tgataaactt gccgtccata tcaaacacat tgaccgccga agcatcgtcc
     4981 aaatcgtagc tgacccgtac ctcgtccttg tgatactcca ccagctcggt tgagaaataa
     5041 gagttgttga acaaatccag ccaaccgcgc tgtacctttc gcacttcctg cggcataaac
     5101 atcgtcgcca gctcttccgc cgacaacata tccggcgcaa tcccgtcctg ttccagcctc
     5161 atttcccgat aagccttcgg cgaataatgc ccgccgtccg gatgtcgggg cagctcgccg
     5221 tgcgggcggt tgttgtattc gtcgatacac ttggccacat ccgcgataaa gcgcgaccag
     5281 ctcggcagtt ttttcaaata tttctgttgc tcctccgtca aatccttgcc tttttccaaa
     5341 gcattaaacg cactttccat cttgcggtac atcaggttct tcgtgctgct gtccattcct
     5401 gcgcccgcaa acatctcata ctggcgcgcc atctcaatca gattgtcttt ccaccatcgc
     5461 tcaatgatgc ctcggccttg cgggttgcct gcgatacccg tttcatgccg gatacccaat
     5521 cgggacgtaa tacccgtgat ttcatgatct atcgtcttac cagtttggcc gccaccgtta
     5581 tccgagtagt agataatcgg caaaccaaag tgcttgaccc cgatacgcag agcgtccgat
     5641 accgccacac aactttcagc caacgacacc gaaaaaccca ccacaaaccg cgtacaacca
     5701 tcaataatca ccgtcacttc cggcttaaac ggcctgccgt gtaccggatg tgccaccttc
     5761 gctttaaagc tgtggccgtc gccaatccaa acatcgttag gcttcaaagc cccccaatca
     5821 cgtttcacat aaggcagcag cgatttataa gccgcccccg ttttcctgcc gcgctcctgc
     5881 ataatcaacg ggagcttgtc ccaaacgcgc cgcaccatac tcaaattagg cacatcattg
     5941 accggcatat tttccgcttc cgcccactgc acaaaccggc ggtagctgtg cgccagcttt
     6001 ggcgcggacg gaatattgtg aaactgcata aacatcggca accatccgta actttcaatc
     6061 ggcttgaccg ccttcgtcgt cttcggagcc aaagcaacca accgctccgt cgcgttttcc
     6121 gctttcaaat aagcagaaat ccagccgtct aaagtacgtt cgccaacctt cgccgaccgg
     6181 ctgcggtcat tggccttttc caagttccca agcgtgaccg cgtccaattt accttctgcc
     6241 agcaagccca aaaactgagc caccgcagcc ttggcagagc aatcgtattg gtatttaatc
     6301 cccaacaccg ccgccaccac cgcacatcgc gcatccgcca ccgaccgttg tttctcgttc
     6361 aacagcttgg ccgcttcagc cagtgcttga gccgacatcg ccgtccccgg tctgacttgg
     6421 ggcagggttt tcggcatctt ctccgccagc tcgtccgact gccgtttcat gatggctgct
     6481 cggatttcgg cagggagggc atattcaaca ccaccaccct taccttgacg ccttctcgtt
     6541 tgccaaccat ccctttgcgc tttttttctc agccctatta ctgttcttgg catatttggc
     6601 aacccaaaat taagcaggct tttgagatcc atatattcca tttcaaccca ctccgagcat
     6661 ctctttcaat tcagctatct ttttgtctcg acaatctgtt ttcttgtaat gcggaaaaac
     6721 ttcaactaaa tctttttgaa tcactttcgc gattgcttcc gcaattcttt tgcttcctcg
     6781 tccatttctt atgacctcag aaacagattg gttagttacc cctaaagccg aagccaacat
     6841 tcttgccgat atattttttt cttctaagga tttatagatt tcgttcgctt gcataatgcc
     6901 tccatgatag aatccattac ttacttacct tacaggcttt aatgcctgcc ttaactgtta
     6961 cttggatgat atacacaaaa tatttggtat accaaatata tttggtaata ttttgcgatg
     7021 tttttatgga acgcattgat ttagcattga atttagttga agagagaagc cgacttggat
     7081 actctcgaaa gaattttgct gagcaaactg gatgcagtgc agaaagtttg agactgtatg
     7141 aaagcgggca ggtcaacatt cctgcggact ttttacttgc tgctgtccaa ttaggggtgg
     7201 atattcaatt tgtctttaca ggcattcatt cggcaaattt aaatgctgtt tcagacgacc
     7261 ttaataaaaa attggacgac gctattggca aatcaaacgt tgagaatcag attaacggca
     7321 acgtcagtaa cgctgttatc gcctcatctg gttcggttgt tcatcaaatt aatacccaaa
     7381 atcacgtcat cagaacccgt gtcgattcaa aacccggcaa ggaacatata acgcttgagc
     7441 aagcttcaaa gctgcaacaa cttgttaaac aagttgccgc agcagaagag attgccaaac
     7501 ggtcgccaaa atcaatccgt gcaatttggg ctagtctgaa tgctcactgt aaagttccaa
     7561 gttacaaact aatcgcattg agcgattacg acaaagcaga aacttatctc agaaaatggc
     7621 tgggtaggtt gagcaataca gcaacatcaa agaacaacga tcccgactgg cgaaaaaaga
     7681 aatacgctta tatcaaatta aatgtaaaac agcttgagct tgaagattgg ttgaaaagtt
     7741 acctcgaaaa aaatttctca gtagaaagcc tgactgaatt aagtgatgaa gatttgcaaa
     7801 aaacatatgc agcagtatcg actaaaaaac ggaaaaaata ggcttaaaca tgaaactccg
     7861 cctttaaatt caagtttcat gtttagctta tggtcttcag atgctttttt agatgtatga
     7921 tattaaaaga ttttttcaaa catggaacta ttatacaagt tccatgttca gtttctagtt
     7981 aaagcctttt ataaacattc taatcaacca cgccgaaacc gagccgcgcg acggcctata
     8041 tgtcttgcgt ataggcaatg acctctttgt caaacgtgtg caacgtatac cgggcaaact
     8101 gcttgtaacg tctgaaaatc cacgttacgc accgtttgaa atagatttga gtaataccca
     8161 agacgacatc gccatcatag gccgtgttga gtggtatggc cggagtattg attaaatcca
     8221 ttttaaaaac ctcttaaaac agttttaaaa attccctaat ctcgtcagct ttaaacagaa
     8281 aaccgcgcat tccggcgcgg ttttgtgaaa aagctggcgt aacttttccg gatacaaaaa
     8341 aacgccgaaa tccacgtctt ccgaagattt cagcgttttt ttactctatt tgttccttgt
     8401 gcaaaaacta acagtccccc acaggaaaac gtcgccctgt tccgccacaa cggcgaagtt
     8461 ttattggacg gcatcaacat caaactcaaa agcggcgatt cgctgctgat acgcggcccg
     8521 agcggttgcg gcaaaacctc gctgctgcgc gcgctggctg ggctttggcc gttcggcagc
     8581 agcggcaaag tcagccgtcc gccgcatcaa gacatcctct tcctgccgca acgcccgtac
     8641 acggcacagg gcagcctgcg cgacgcggtt tgttatccgg acatcgacaa acagcatccc
     8701 gagttagccg aagccatgaa cacctgccgc ttgggttatc tgattgacaa attggacaaa
     8761 acagacgact ggcagcacaa actctccccg ggcgaactgc aacgcgtcgc cttcgtccgc
     8821 gccctgcttt ccaagcccaa aatcgtcctg ctcgacgaag ccaccgccgc tttggacgaa
     8881 ccgaccgagg ccctgctcta ccgcgcactg aaacaaaaac tgcccgacag catcatcatc
     8941 agcatcggcc accgcagcac gctcaacgcg ttccacaata tgcacctcga tgtcggcaac
     9001 gttgcctgcg gttgaacgat gccgtctgaa gccgttttca gacggcattt ttcaaattga
     9061 taaagcacgc gaaaacaacg tacaataaac ccgtttacca cccctcggaa tgccgaaccg
     9121 tgaaccagct tattttcgac tttgccgcac acgactatcc gagtttcgac aaattcctcg
     9181 gcacggaaaa cgcggaactg gtctatgtcc tccgacacaa acacggacag tttatctatg
     9241 tctggggtga agaaggtgcg ggcaaaagcc atcttttgca ggcgtgggtc gcacaggcgc
     9301 tcgaagccgg caaaaacgcc gcctatatcg atgccgcctc catgccgctg accgatgccg
     9361 cgttcgaggc ggaatacctc gccgtcgatc aagtcgaaaa gctgggcaac gaagaacaag
     9421 ccctgctttt ttccatcttc aaccgcttcc gcaacagcgg caaaggcttt ttgctgctcg
     9481 gttcggaata cacgccccag cagctcgtca tccgcgaaga tttgcggacg cgtatggcgt
     9541 actgcctcgt ttacgaagtc aagcccttaa ccgaccaaga aaaaatcgac gcgctcgcca
     9601 gtatggcggc ggcacgccaa gtaaccgtcg attccgaaat tttcgaatac ctgctcaaac
     9661 actggcggcg cgatatggac agcctgatga tgatgctcga cacgctggac aactacgccg
     9721 tcacaatggg caaacgcatc accctgccgc ttttgcgcca gcttttgaaa caacaggaaa
     9781 cccaatgaaa aacctcgcca tcttcgacct cgacaacacc ctcatcaaca ccgattccga
     9841 ccacgcctgg ccgcaatacc tcatcaaaaa aggcttggtt gacgctgccg aaaccgaagc
     9901 gcaaaacgaa aaattctacc gcgactacca aaacggctgc ctcgacatcg acgcattcct
     9961 caaattccac ctcgccccgc tcgctcgtta cagcaaagaa gagctggcgg aatttcaccg
    10021 cgaatttatg gcggaataca tcatccccca catctcgcct atgcagcgta tgctggtgca
    10081 gagccaccaa atggcaggcg acgaaaccct tgtgatttcc tcaaccaacg agttcatcat
    10141 cacccccgtc tgccaccttt tcggcatcac caacatcatc ggcacacaac tcgaaaccgg
    10201 ttctgacggc cgctacaccg gcaattacat cggcacgccc agcctcaaag aaggcaaaat
    10261 cacccgcctg aaccaatggc ttgccgaacg cggcgaaacg ctccaaagct acggcaaaac
    10321 ctatttttac agcgactcca aaaacgactt gccgctgctg cgccttgtca gcgaacccgt
    10381 cgccgtcaac cccgatgccg agctggaaaa agaagccaaa gaaaaaggct ggccggtttt
    10441 gaatttcaaa tgacgcaatg ccgtctgaag cccgattcgg cgttcagacg gcattgaggc
    10501 tgaaatccaa aaaactcaaa gccacaggaa tttatccgga aaaacaaaaa acacaggaat
    10561 tcatcgggaa aaacaacaat ctttccaccg tcattcccac gaaagtggga atcttgatcc
    10621 gtccgcacgg aaacttatat ttcgtcattt gcacccgaca taattcaata ctttattttg
    10681 caataaatta acggatgctg acaaattaga ttggattaat taccctatta tccatattgg
    10741 gttatcgggt tgctaattga gcagagcgta gcaaaccata aagtgagtgt ttccacccac
    10801 tctgcattta tagtggatta aatttaaatc aggacaaggc gacaaagccg cagacagtac
    10861 agatagtacg gcaaggcgag gcaacgctgt actggtttaa atttaatcca ctatactatg
    10921 aagtggttat taaatatgat aatgagacct attaaattta gtatggtaaa tacgttatta
    10981 tttattgtta tatgtagttc attttttgat ctgctcgttc aattatgtac aattttattt
    11041 catagccaaa aaatatactt tattacatta tttttattat ttatttttaa ttttgttaca
    11101 aaatctatct atatggcaat tatttatcct attttatatt tttttacgat aaaaaaatat
    11161 tatccttact ctaggaaagt gataattcta ttattattag cattatctat atattttagt
    11221 tttatggact tttacttttt ttccatatat tcagataacc ttagctatga aacggagcct
    11281 ttacatttat acatccctat tattattaat tttttctcac ttttagtttc taattttatt
    11341 ttatctttta tcaacaagta aatgtatata caaaatacaa tccaaaagat cctaagcaaa
    11401 ccaccaatcc cacaccaccc ttaccggttc aaatccgcac aaaaacccac atcccgtcat
    11461 tcccacgaaa gtgggaatct aggac
//
LOCUS       DAWKQJ010000051        11465 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000051 DAWKQJ010000000
VERSION     DAWKQJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.051"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="WJL20_001032"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="WJL20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ7683196.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQKQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WJL20_001033"
     CDS             complement(1966..2568)
                     /locus_tag="WJL20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ7683197.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLMEMPPTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJL20_001034"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJL20_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ7683198.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJL20_001035"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJL20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ7683199.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WJL20_001036"
     CDS             complement(4526..5488)
                     /locus_tag="WJL20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ7683200.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJL20_001037"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJL20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ7683201.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6123..7013)
                     /gene="ubiA"
                     /locus_tag="WJL20_001038"
     CDS             complement(6123..7013)
                     /gene="ubiA"
                     /locus_tag="WJL20_001038"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ7683202.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGMFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVLPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7043..7519)
                     /locus_tag="WJL20_001039"
     CDS             complement(7043..7519)
                     /locus_tag="WJL20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ7683203.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7977..8420)
                     /locus_tag="WJL20_001040"
     CDS             complement(7977..8420)
                     /locus_tag="WJL20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683204.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            complement(8414..8800)
                     /locus_tag="WJL20_001041"
     CDS             complement(8414..8800)
                     /locus_tag="WJL20_001041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683205.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            complement(8824..9054)
                     /locus_tag="WJL20_001042"
     CDS             complement(8824..9054)
                     /locus_tag="WJL20_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683206.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            complement(9021..9668)
                     /gene="bioD"
                     /locus_tag="WJL20_001043"
     CDS             complement(9021..9668)
                     /gene="bioD"
                     /locus_tag="WJL20_001043"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ7683207.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKLVQTGC
                     QNIADDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(9665..10966)
                     /gene="bioA"
                     /locus_tag="WJL20_001044"
     CDS             complement(9665..10966)
                     /gene="bioA"
                     /locus_tag="WJL20_001044"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ7683208.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMAHFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGLLTELADKTVHILKEHNK"
BASE COUNT         2801 a         2985 c         2704 g         2975 t
ORIGIN      
        1 gcacaatcac ttgttcaagt gattaagaaa gcgtgataat acggatttat ctgagttctg
       61 tctattgttt tagactttta tttttagtta ttttttaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaatgac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgg atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttcgccgca
      301 ggatgcgact tgcggaagat gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 tagggctttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg caactttatt cagcggacgg ggcggattgc ctttgttggc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgagg gcaacaggac
      601 gatgtcgaaa cgtgcacctt tcatggcaag gtgttgcata tgctcggtcg tttcgccgct
      661 caaacgctct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcggcggcag cttgcaggct
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcaggct cgatcctgta
      841 tttccgcgcc atccccatca gttcgcccat acgctgttcc tgtgccgcca attcattggg
      901 gttgatgtcg ctgcgacctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ttcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcag cctgcaacag ctcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgagg gcttcccact cgccttgttt aatgtccaac tgattcaatt cgttaaactg
     1201 ccattccaga cgctcttgtt cgagtgcaat gttttctgcc tgtttctgcg cttcctgcaa
     1261 tgcttttttt gagtttgccc agttttgata aagctgcctg acggtttccg cctgcgccct
     1321 gctgcccgca aatgcgtcca acaattcgcg ctgggcggct tcctgattaa gcgaatgatg
     1381 ggcgttttgc ccatggatgt cgataagctg gctgccgacg gctttgagtt gcgccaaggt
     1441 agcggcctga ttgttgataa agctgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tcttctccgt cgtttaaaag cccctgttca tacaattctg cttttaaaac
     1561 aggtaaatgg gaaatatcaa acaacgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggctttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgccg gtctctccgg tcaatacggt aaagccgctt tgaaaatcca gattcagatt
     1741 ttcaacaatg acaaaatcac gcaaagaaag tgttagaagc atcgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttataactaa gattttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtaggc ggcatttcca ttaaatcggg
     2041 taaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaagctgt tcaaacccaa
     2101 atcgctgagg aacgttgccg ttgtcgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt atgccctcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtaacg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcctg
     2461 cgacagtggc ggcacacaca attcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatta gtgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggtttctgt cctttgcgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccaattttg ccttcgcgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcaaca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca acgcgttgcg tccgactgcg ccgccggcgg
     3061 caaggttgta gccgccttct tcgacgatga ttttcggcca cagcattccg ggggtgtcgt
     3121 acagccagaa gtcatcggcg aggaagaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcaaatcaaa acgcgcaggg gtttgtctat gccttgccgg tggggaatca
     3301 tcgcgcggca ggcttgggtg attttgccgt gtgcgccggt ttcggaggaa tcgagggcga
     3361 tggcgcacgt gtcggggcgg ctgttgtagt ggtcaagcca gacctttgtg cgttcggggt
     3421 cggcaagatc ttgtttgttt aggattttaa gtttgggttt gcctttggaa agctgggcaa
     3481 gcagggggtt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgctttttgc acgctcggcg atggcttttt tcgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag actgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tactcagagg gtggcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcgatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgctc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggatttgccc gaaccggaca acccgctaat caggacgatt ttcatgattc tgttcgtttt
     4561 ctttaagttg tgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gaagaatata attgcgcacc gccgcttcaa ccaatacggc aaggttgcgt ccgaccgcga
     4681 caggcagcgt taccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtacggatac
     4741 tcaaacggtc gagctgcttc atatactcgt cgtccgcctc gaccaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaccgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagctccaa cgccaattcg ctcttaccca aacccgaatg tccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccagg gtgcgttgca
     5101 ggtaaatccg caacacatcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atggcagtag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 tgaccaaaga catagaaatg tcgaacaggt cgccgaactg ataacccgtt tcccccgatt
     5281 cgagacggtt cagatattcc gattccgcca aaccgaccac ttggatttgg ttggggtgga
     5341 taaaattcag gtgtccgacc agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccagg caagttgtaa cttgtattgg ttgtcgtcaa
     5461 acagccggcg gacggaaata ctgggcatat tattcttcag tcaaaatggc gcgggcttct
     5521 tccgcagaag aaaccgtcat cagcgattcg cggatgcttt tttgggaaaa cttgccggcc
     5581 agtttggata agacttccaa atgttcgcca gttgcgtttt ccggcaccag caagataaaa
     5641 atcagggaaa ccggcttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 gacaatgagg attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg
     5881 cccacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt tcagacatcg caaaacagga agattgtacc gactgccggg gcaaatctca
     6001 atcccgcata cggtacgggc tgacataaca cagcgtttta aaaacatatt ttaacgcttt
     6061 tcggcacaga tagaaatgcc gtccaaagca gtttacggct cttcagacgg cattgccctg
     6121 ccttatttcg cgaaaaacgt atgggcaaaa atggcggtaa accacaccca accaattctg
     6181 ttgtttgcca aaaacgtttc aaaacagatt tgccggacgc ggcttttgat ggcggcatat
     6241 tggcggtatt gcagcagcag gacgatgggg attgccgtcc aatatgccca tgccgcaccg
     6301 ataaccgcac ccaatactgc catcagcagg gtaaagccgc catgacacag cataacggcg
     6361 gcgatgtcgt aacgcccgaa cgtgacggcg gaggttttga tgccgatttt caaatcgtct
     6421 tctttgtccg ccattgcata aaccgtgtca tacgccagag tccataacac attggcggca
     6481 aagagtatcc acgcttgagg cagcacgttt ccggcaacgg cggcaaacgc catcgggata
     6541 ccgaaggaaa aggcaagccc gagatagagt tggggaatcg gaaaaaaacg tttggtaaac
     6601 gggtaagtca gcgcaagaaa cagcgcgggc aggctcatca gccaagtcag atgattcagc
     6661 ggaatcaggc acaatgcggc aagcaggcac aaaaatgccg tcagcagcag cgcttctttt
     6721 tttccgaccc gaccctgtgc gaacggacgg tttttggtgc gctcgacagc accgtcaaaa
     6781 tcgcggtcgg caaaatcgtt gatgacgcaa ccggcactgc gcattaaaaa cataccgatt
     6841 gtaaacgccg ccaataccgc caaatcggga atgccgtctg aagccagcca caatgcccag
     6901 taggtcggcc acagtaaaag cagcgtccca atgggcttgt ccgcccgcat caggcgcagg
     6961 tacacatcca aacggtcgga cagtcgtaaa aataaagggg atttaggatt catattgccg
     7021 cacagcttga aaaaacggta ttttatccga taaagcgttt cagttcgggc agaaaatact
     7081 cggtcagcag catttcctcg ccgtgacggg aaaaccgaga acgccgcgcg gcaaagtacc
     7141 gtccgcatcc ttcgccggaa acggcaaact caaacgccga acgcgcccct tccaaatcgg
     7201 cttgaaacag acgctcgccc aaagggcgcg tgccgcagtc caaaatgttt tgccaaaacg
     7261 ccgaacctat ccggcactcg ctccttgccg cgacaacagg gataccgtcc agcttcaaca
     7321 aaacttcgcg caccagcctc cctccgcatt ccgtctccaa ttcgcccagt ttcagcagtt
     7381 ccaccgaaaa tgtatgcggc aaggctcgca atgcggcggt cagcgaccgg gctttcaata
     7441 accggctcat cggcaggccg atgccgtctg aaacaggggc gggcaggtcg ggcagccatt
     7501 cctcaaagcc gtaacccatt attaccattt tgttgaatat cctttattat taagcataca
     7561 tcttgcatta tccccaatac aatttgtcga tttgactgca ttatcattgt atttattatc
     7621 ataatatttt tttataatat attgataatt acccttttca tcaggtaatt gatataattt
     7681 atatatttca ggacaagcaa cggaaaggca acttctgaaa tcatcaattc gttgttcgtt
     7741 agtcgtatta tttttaacaa aatattcaat atcgttgttt gctcttatcg tcatatcatc
     7801 aaaaggataa ttacaagacg aagttgcaaa aaatcaagga ttacaagaag ttgtcattac
     7861 cattataaaa acaattaatg acaactttaa aacaacgcgt aatcataaat caatgcccgc
     7921 ttaatttcat aatcacttgg ataaagtcgc tatcaactat atcggcaata gcaaatttaa
     7981 tggttttcat ttaacaattt ctccatttct tttttataat ctattttctg aaattctgcg
     8041 gttttttgat aataatcata accacccaaa gaactcaaac aaataatgtg catatttcta
     8101 actgcattat aagccaattc cttatcattt cttggaatac ctttttcttt aagcatgaaa
     8161 atgtaattat caaaatcttg cggaatattt aattcccgta tattggaaaa atacatatta
     8221 gcacaatatt taatatcact cctaacttga tttatgcttt tattaggatt ttttctaata
     8281 acattttcaa tatcagaact tacaggacaa tgaacgcttt gtggggcaac aaagcatttt
     8341 tcattatcaa tagttgtgta ataacagccc tgcattatca acaatacaag ggctgttgat
     8401 aataagcgga acattaccat tttgttgaat atcctttatt atttttcatg cattctccaa
     8461 tctttccgta gcatgtcatg acatcatctg cattcttata gcccttttta taatcatctt
     8521 tatattttct acgaataatc tccctataat ctccattttc atctggtagt tgatattttt
     8581 gataaacttc tggacatgca taagaaatac aatagcgtaa atccgcaatt cttgtctctt
     8641 tgctcgtatt attcttaaca taatattcat cgtcgctatt tataggatag ttttttctaa
     8701 aagaataatc acaagaagaa gttgcaaaaa agcaaggatt acaagaagtt gtcattacca
     8761 ttataaaaac aataatgaca actttaatat agcatttcat aacaaccacc aatatataga
     8821 taatcaatga ccagtaattt tcataatcgc ttggataaaa tcctgatcga ctatatcagc
     8881 aatagcaaat ggagcggcta ctggtatcgc tgcaacagtt actttacttg aaatagtttc
     8941 atctttttta tttgttgttc ttctatttgg cgttgtattt ccatattcat catataggtg
     9001 ttccatattt ttcccaatct ttataccgcg tctgtttttg ccaactccat ccattccgct
     9061 tcggaaaaat cggctttcag acggcatttc aagtagctca ggctgtcttg ggcgatgtgt
     9121 gcgtcgcggc tgtcgtggat gtggttgaac acgaggctgt gcaagcgaat gccgtattgt
     9181 ttgagcgcgg cgaaactgag cagggtgtgg ttgatgctgc cgagccgtcc gctggtaacg
     9241 aggatgacgg gatagccttg ctgacggata taatcaatgg ttaacaggtt ttccgtcagc
     9301 ggaaccatca atccgcccgc gccttcgacc aaaacgactt cgtactgcgc cgccaattct
     9361 tgtgtggcgg tacggatttt gtccaagtcc aaatccctgc catccagacg ggcggcaagg
     9421 tggggcgagg cgggatagct gaagatttcg ggcatggtca acccttgttt gtcggcttcc
     9481 tgcatgggta tgcccatgat tttgcggtgg acggcgatgt cgtcggcgat attttggcaa
     9541 ccggtttgca cgagcttttg cgtaatcaca cttttgcctt gctgcaacaa ttgttttgcc
     9601 aatacgccgg tggcgacggt tttgccgatg tccgtatcaa tgccgctgac gaagtaaacg
     9661 cctttcattt gttgtgttcc ttcaaaatgt gtacggtttt atcggcaagt tcggtcaaga
     9721 ggccgtctga aataatgtaa ggcggcatga ggtacaccag cctgccgaag gggcgcaccc
     9781 aaataccctg cgccacgcaa tccgcttgaa aatgcgccat atctacgcct ttatccagct
     9841 cgatcacgcc gatggcaccc aaaacgcgta cgtctttcac gccgcgaatg tcccacgcgg
     9901 ctttcagacg gccttttaag atgctttcaa tgcggcggat atttgcctgc cagtcttgag
     9961 acaaaagcag tttgaccgaa gcgcaggcaa cggcacacgc cagtgggttt gccataaatg
    10021 tcggtccgtg cataaacacg cccgcctcgc cgcgcgaaat cgtttcggta actttttgcg
    10081 aagtgattgc cgccgccagc gtcatatagc cgccgctcaa acccttgccg atgcacataa
    10141 tatccggcac gacctccgcg tgttcgcagg caaacatctt gcccgtgcgc ccgaatccgg
    10201 tggcgatttc atcaaaaatc aaaacgatat caaactcgtc gcacaaatcg tgcaatccgc
    10261 gaagatactg cggatgataa aaatacatgc cgcccgcgcc ctgcacgacc ggttccaaaa
    10321 taaaggcggc aatgtccgta tgatgcgctt caaacaaggc gcggacaggc tgcaaatccg
    10381 cctcgtccca ttcatcgtcg aaacggcttg tcaggttatc gacaaaataa cgctgcggca
    10441 acgcgctgcc gaaaatatgg tgcatccccg tttccggatc gcagacggac atcgcgttcc
    10501 aagtatcgcc gtgatacccg cgccgcaccg tcgccatatt ttgttttgca cgcaaacccc
    10561 gcgcctgctg gtattgcacc gccatcttca acgcgacttc caccgaaacc gaacccgaat
    10621 ccgcataaaa aatacgatcc agcccctgcg gcaaaatccc gaccaacaat ttgcccagct
    10681 ccaccgccgg ctcatgcgtc aaaccgccga acatcacatg cgacatctgt ttcatctgcg
    10741 tctcaaccgc ctgattcaaa acgggatgat tgtaaccgtg tatcgcacac caccaagacg
    10801 acatcccgtc aatcagccgc gtgccgtccg ccaattcgat aaacacccct tctgcacgtt
    10861 tgacaggata aacgggaagc ggatcggtca tggaagtata gggatggagc agatgggtac
    10921 ggtcgaaatc aagcaatgat gatgtgtgtt gatgttcaga cggcataagt ttctctcttt
    10981 tcttattgct gcattcaaac gcaaaacgcg tattctactc cgacagaccg cttcccacac
    11041 ctttccatcc gtttcgggcg taaaaccacg caacaaatcg tccgcagtat aagcgcacac
    11101 cgtttcgcat tccccaagcc cgattggaat cagacgaccc aacgcccaat attgtttcac
    11161 acagcctccg cccaacgctt caatcagtca tagacaaaac ggcaatccat acgatacaga
    11221 tcatagcaac agccatcgca acagcgttag caaaatcagg ggactccgac ataggcgcat
    11281 agcatctacc gatgcacagc tcctcattcg gctctatgaa taccataccc atcacaaaat
    11341 ccaccgccaa aaccaggcac ggcttcttat acttatgata gatttccacc atcctgcccc
    11401 atatatccca aacattcata ccgcatatcc cgcaggcaac aaattccgat tgaaggttac
    11461 agccc
//
LOCUS       DAWKQJ010000052        11417 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000052 DAWKQJ010000000
VERSION     DAWKQJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.052"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1207)
                     /gene="apbC"
                     /locus_tag="WJL20_001045"
     CDS             complement(128..1207)
                     /gene="apbC"
                     /locus_tag="WJL20_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ7683209.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1418..1493
                     /locus_tag="WJL20_001046"
     tRNA            1418..1493
                     /locus_tag="WJL20_001046"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1451..1453,aa:Lys,seq:ttt)
     gene            1572..2081
                     /locus_tag="WJL20_001047"
     CDS             1572..2081
                     /locus_tag="WJL20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ7683210.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVGAYMEKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2424..2864
                     /gene="nadR"
                     /locus_tag="WJL20_001048"
     CDS             2424..2864
                     /gene="nadR"
                     /locus_tag="WJL20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ7683211.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            2979..3479
                     /gene="hpaC"
                     /locus_tag="WJL20_001049"
     CDS             2979..3479
                     /gene="hpaC"
                     /locus_tag="WJL20_001049"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ7683212.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3495..4190
                     /locus_tag="WJL20_001050"
     CDS             3495..4190
                     /locus_tag="WJL20_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ7683213.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4421..4852)
                     /locus_tag="WJL20_001051"
     CDS             complement(4421..4852)
                     /locus_tag="WJL20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7683214.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5027..6703
                     /locus_tag="WJL20_001052"
     CDS             5027..6703
                     /locus_tag="WJL20_001052"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ7683215.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6817..7908
                     /gene="ychF"
                     /locus_tag="WJL20_001053"
     CDS             6817..7908
                     /gene="ychF"
                     /locus_tag="WJL20_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ7683216.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8178..10049
                     /locus_tag="WJL20_001054"
     CDS             8178..10049
                     /locus_tag="WJL20_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7683217.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGVSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFLL
                     WKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARK
                     SPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRYRQQRNLA
                     VGRCCAAAGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKK
                     FFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHC
                     IEKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNN
                     TGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWAATMVSADACAYVEGYAS
                     RVFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLH
                     KLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAA
                     KYPNVYIIDAAAYIPADFQIGGLPVYSDTDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            10111..11406
                     /gene="tyrS"
                     /locus_tag="WJL20_001055"
     CDS             10111..11406
                     /gene="tyrS"
                     /locus_tag="WJL20_001055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ7683218.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2565 a         3049 c         3162 g         2641 t
ORIGIN      
        1 ccgtcattcc cacgaaagtg ggaatccgga acgtaaaatc tcaaaaaaac cgttttatcc
       61 ggcaagtttg cccgccggca ggcgcggggc ttgggacggc attgctgttg cggtttcgga
      121 cgcggcttta ttcgacgaca attttaggga accggctgct gaagtctttg cctttgtcgg
      181 caatgcccag ggcgatttgg aatgcggcat cggtgtagat tcgggcgatg gcggggtgtt
      241 cgtcgaacag ttgcgccggt gtgccgccgt ccattgcttc gcgcacgggc aggcttaggg
      301 gaagctgtcc gagcaggggg acgttgaggc gtgcggctaa atctttgccg ccgtccgtgc
      361 cgaacagggc ttcgctgtgt ccgcagttgg tgcagatgtg gacggacata ttttccaata
      421 cgcctaaaat gggaatgttg accttgcgga acatatccac agccttgcgc gcgtctatca
      481 gcgcgatgtc ctgcggcgtg gttacgatga cggacccggt aacggggatg cgttgggaca
      541 gcgtgagctg gatgtcgccg gtaccggggg gcaggtcgat gaaaagataa tccacttcgt
      601 cccactcgct ttgaaacatc agttgctgca aggcttggct gaccatcggc ccgcgccaga
      661 cgacggcttg gtcggtatcg acgagaaagc cgatagacat gacctgtatg ccgtctgaag
      721 attcgacggg aatgagtttt tggtttttct gatcgggttt gcggtcgtcc acacccagca
      781 tcgtgggctg gctcgggccg taaaggtcgg catcgagtac gccgacgcgc gcgcccatgc
      841 gcgccattgc ggcggcaagg ttggcggtgg tggttgattt gcccacgccg cctttgccag
      901 aggcgacggc gatgatgttt ttcacgcctt taatagtggt aacgccgggc tggactttgt
      961 gtgtgccgat ttcagtgtcc atggacagat ggatgtgtgt gtcgcctgtc agcggcatca
     1021 ggatttcctg tatgcggtcg gctgtttctg aggcaatgtg cgcgacgggg aagccgaaat
     1081 gcagggcgat atggatgccg tctgaacgct gttcgaccga acggacggcc ttttcgccgc
     1141 cgagcgtgcg tgccgtattc ggaacggcga cggtgtcgag gagggtgcgg atgttttgta
     1201 tgttcataat gtgtgtttct gtgtgcagtt tgggaaacgg gcggaaagtg tacattgatt
     1261 tcgggcggtt tgcttttcct tgtgcgaact tttacacggt atgcggcgga ttgtgaaaat
     1321 ccgttgtcaa aatatcccgt gcaaacagtg gtctgcaatt aagcctgtaa aatttgcttg
     1381 acgaggtagg atggtgctgt ataatgcacg gcttatcggg tcgttagctc agccggtaga
     1441 gcagcggact tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataacc
     1501 ggaagccaag ttttgagact tggctttttg tttgcctgcc ggttgcgcgg gcgtaccgtt
     1561 tgagagagcg tatgaaaaag aaactgcttt cgggcatcaa atttgccgtt cagacggcat
     1621 tggtgttttt attggtgtcg ctgtttttgg attggatacg caagcccgaa gagccggccg
     1681 gtgcggcagg gcggcctttg accctgctgt cggggcagcg gctgactttg ggacagttta
     1741 gccgagataa ggcggttttg gtgtattttt gggggagctg gtgcggcgtg tgccgttatc
     1801 agtcgccgat aatcgatgat ttggcggcgg acggcgtgcc ggtcgtcggc gtggcggtac
     1861 gttccggcag ttcggcggaa gtgggggcgt atatggaaaa acgcggcttg ggctttccga
     1921 cggtcaacga tgaggacggg ggtttggcaa ggtcttggcg gattgtcgca acgcctgccg
     1981 tcgttttggt caaaaatggg aaaatggttc actatacgac gggaattagc agttattggg
     2041 gcttgcgcgc acgaattttt caggcggatg ttttcggtta acttttgttt ttgttaaact
     2101 ttacgctatg tagagaatca aacggcatat accttgctcg tctgatgcgg cggcttttgt
     2161 tttttctgga agaaagttct ttcgacagta aatgtaatac tttgttgaga ttgtagggaa
     2221 aatagactaa gatataacta ttaaaatatt tttagatagg attatcggaa ttaaagtctt
     2281 ttatacccag tcgtccgatg cggtttatag cgtattgttg ctatatgttc gttttgttat
     2341 ataacggttg catcaaaatt tacgcccaca ggctttcccg acggtttgaa agtttgattt
     2401 tcgataactt ggagacttga acaatgccta cccaatcaaa acatgcgtct atcaatatca
     2461 gtctgataca ggcaagggaa gccctgatga cccaattcag gcctattctg aatcaggcga
     2521 atattaccga tcagcaatgg cggattatcc gtcttttggc ggaaaacggc acgctggact
     2581 ttcaagattt ggcgaatcag gcgtgcattt tgcgccccag cctgaccggt atcctgaccc
     2641 gccttgaaaa agcgggtttg gttgtccgcc tgaaaccttc caacgaccaa cgccgcgttt
     2701 ttctgaagct gactgccgag ggcgagaagc tgtatgagga aatcggcgaa gaagtggacg
     2761 agcgttacga cgctatcgag gaagtgctgg gccgcgagaa aatgctgctg cttaaagacc
     2821 tgttggcaga acttgccaaa atcgaggatg cgttgaactc gtaatacgcc gtaacgcgcg
     2881 gaaatgtccg accgacggct ttttgaatca gaactgctgc acatgggggg tgccttgtgt
     2941 gcagcattct tatatagggg acagtttaaa ggggaaaaat ggcggatttg cagaaaactt
     3001 ttcaaacttc gttccgtgat gcgatggcat cttgtgcggc aggcgttcat gtcatcacga
     3061 cagacggtgc ggcagggcgt tacggcatta caatgacggc ggttacaccg gttacggacg
     3121 agccgccgac cgtgatgctg tgcatcaacc ggagtgcgcg aatcattccg atcctgtcgg
     3181 aaaacggcag cctctgcatc aatacgctgg cggacgaaca tcaggatgtt gccgaacatt
     3241 ttgccgggct gaccgggctg tcgcccgaag agcggtttgc ctaccacatc tggcatcgcg
     3301 gcaaaacggg acaacttgaa atagagggcg cgttggcgca cctgcacggg catattgtcg
     3361 gcaaacatga aatcggcacg cattttgtgt tttacgtcag gctcgacgaa atcaaaaact
     3421 gcgggtgcaa acgccccgcg ctgctgtatt tcagacggca gtttagattt ttagactgat
     3481 attcggacag atatatgaaa gcgatgatac tggcggcagg acgcggcgag cgtatgcgcc
     3541 ctttgaccga taccactccg aagccgctgc tcgatgtggc gggtaagcct ctaatcggtt
     3601 ggcacctatg ccgtctgaag caggcggggt ttaccgaaat cgtcatcaac cacgcttggc
     3661 tgggccggca gatagaagat gctttgggcg acggctcggc ttatggcgtg aacatcgcct
     3721 attcgcccga acccgcaggc ggtttggaaa cggcaggcgg catcgcgcag gcattgccgc
     3781 tgttgggtgg gcagccgttt ttggtggtca acggcgacgt gctgaccgac atcgatttta
     3841 ccgccgcgtt tcaggcggca tcgtccctgc cggaacatat ttccgcccat ctgtggctgg
     3901 tggaaaatcc gccgcacaac cccgacggcg atttttccct gctgcccgac ggcagcgtgc
     3961 ggccggaagt aaatggcggc aacggattga cattcagcgg cgtgggtatt taccgtcctg
     4021 aaatgtttga cggaatcgaa gcgggcagtg tggcgaaact cgcgcccgta ttgcgtggcg
     4081 aaatgcggca aaaccgcgtg agcggtcaga agcatacggg cttgtggctg gatgtcggca
     4141 cggtatgccg tctgaaagag gctcaagccc ttgcaggggc ttggaagtga aataaaacgg
     4201 tggcttgccg agccggctgc cgaacggttc cgaccggatg tccagcatat tttcgtggag
     4261 gcgtgaaaat agggcgttcg gggctttccg tccaacaaat gccatctgaa gcgcggcggt
     4321 ttgccgtcgg cgcggtgtcg gattttattg ggtaactttg ttcagaccgc cgcccgtcag
     4381 attgtcaaaa cccggtttca ggcggtatgg cggattcggt ttaacgtttc aacgccagca
     4441 ccaacacgcc cgccgttacc agccccaagc ctatccattc ctgcgtgttc gggcgttcgt
     4501 ccaagaaaac caccgccatc agcgcgacca agaccaggct gaatttgtcg atgggggcga
     4561 cttgcgaggc gttgcccagt tgcagggctt tgaagtaggc gagccaggat gcgccggtag
     4621 caaggccgga taggataagg aatgtccagt tgcgccccgt aaagccgttc acaccctgcc
     4681 atttgccggt gtaggtcaaa aacaatacca aagcggcgag gatcaccaag gtgcggataa
     4741 aggtggcgaa atcggaatcg atgccctgta aacccatttt ggcgaaaacg gcggtcaatg
     4801 aggcgaagcc tgccgatgcc aatgcccaaa acagccatgc gttgctgctc atgttttctc
     4861 cttggattgt gaacaatatg aacggtattt ttgttgcagc gtcaaaaatt tcactgcggg
     4921 tttggtgcgg ataacgttat aatatgcctg atattatttt caatccacct gtttgtcgcc
     4981 tgatgctttc agacggcatg tccctcctca tttctaaagg aaaatcatga gcttcaaaac
     5041 cgatgccgaa atcgcccaat cctccaccat gcgcccgatt ggcgaaattg ccgccaagct
     5101 gggtctgaat gccgacaaca ttgagcctta cggtcattac aaggcgaaaa tcaatcctgc
     5161 cgaagcgttc aaactgccgc aaaaacaggg caggctgatt ttggttaccg ccattaatcc
     5221 gactccggcg ggcgaaggca aaaccaccgt aaccatcggt ttggcggacg cgttgcgcca
     5281 catcggcaaa gatgccgtga ttgccctgcg cgaaccttct ctggggccgg tgttcggcgt
     5341 gaaaggcggc gcggcaggcg gcggctatgc ccaagttttg ccgatggaag acatcaacct
     5401 gcacttcacc ggagattttc acgccatcgg tgcggcaaat aatctgcttg ccgcgatgct
     5461 cgacaaccat atctatcagg gcaacgagtt ggacatcgac cccaaacgcg tgctgtggcg
     5521 gcgcgtggtc gatatgaacg accgccagtt gcgcaacatc atcgacggca tgggtaaacc
     5581 cgttgacggt gtgatgcgcc ccgacggttt tgatatcacc gttgcctccg aagtgatggc
     5641 ggtattctgc cttgccaaag acatcagcga tttgaaagag cgtttgggca acatcctcgt
     5701 cgcctacgcc aaagacggca gccccgttta cgccaaagat ttgaaggcac acggcgcgat
     5761 ggcggtcctg ctcaaagatg cgattaagcc caatttggtg caaaccatcg aaggcacgcc
     5821 cgccttcgta cacggcggcc cgttcgccaa catcgcccac ggctgcaact ccgtaaccgc
     5881 aacccgtctg gcgaaacacc ttgccgatta cgccgtaacc gaagcaggct tcggcgcgga
     5941 tttgggcgcg gaaaaattct gcgacatcaa atgccgcctt gccggtttga aacctgatgc
     6001 ggcggtcgtc gtggcgactg tccgcgcttt gaaatacaac ggcggcgtgg aacgtgccaa
     6061 cctcggtgaa gaaaacctcg aagccttggc aaaaggtttg cccaacctgc tgaaacacat
     6121 ttccaacctg aaaaacgtat tcggactgcc cgtcgtcgtt gcgctcaacc gcttcgtgtc
     6181 cgactccgat gccgagttgg cgatgattga aaaagcctgt gccgaacacg gcgttgaagt
     6241 ttccttgacc gaagtgtggg gcaaaggcgg cgtgggcggt gcagacttgg cgcgcaaagt
     6301 cgtcaacgcc atcgaaaacc aaccgaacaa cttcaatttt tcctacgatg ttgagctgag
     6361 tattaaagac aaaatccgtg cgattgccca aaaagtgtac ggcgcggaag atgttgattt
     6421 cagcgcggaa gcgtctgccg aaatcgcctc gctggaaaaa ctgggcttgg acaaaatgcc
     6481 gatttgcatg gcgaaaaccc aatattcttt gagcgacaac gccaaactct tgggctgccc
     6541 tgaaggcttc cgcattaccg tgcgcggcat taccgtttct tccggtgctg gtttcatcgt
     6601 tgcgttgtgc ggcaatatga tgaaaatgcc gggtctgccc aaagttcccg ccgccgagaa
     6661 aatcgacgtg gacgcagaag gcgtgattca cggcttgttc tgaacggttt ttgaaaccag
     6721 atgccgtctg aagccgtttc agacggcatt ttttcggaac gcgggcggcg gtatgctata
     6781 atccgccgtt aaatttctct attttcagga aaaaacatga gtttgaaatg cggcatcgtc
     6841 ggtttgccca acgtcggcaa atccaccctt tttaacgcgc tgacccagtc gggtatcgaa
     6901 gcggcaaact atcctttctg taccatcgaa cccaacgtcg gcatcgtcga agtgcccgac
     6961 ccgcgtatgg ctgaattggc aaaaatcgtc aatccgcaaa aaatgcagcc cgccatcgtc
     7021 gaatttgtcg atatcgccgg tttggttgca ggcgcgagca aaggcgaagg tctgggcaac
     7081 cagttcctcg ccaacatccg cgagactgac gcgattgtta acgtcgtacg ctgctttgac
     7141 gacgacaaca tcgtccacgt ttccggcaaa gtcgatccga ttgccgacat cgaaaccatc
     7201 ggtacagagt tggcacttgc cgacctggca agtgtcgaaa aagccatcgt ccgtgaagaa
     7261 aaacgcgccc gctccggcga caaagacgcg caaaaactgg tcgatttgtg caaaaaactg
     7321 ctgccgcatc tggacgaagg caaacccgtg cgttccttcg gtttggacgc ggaagaacgc
     7381 gcgatgctca aaccgctgtt cctgctgacc gccaaaccgg cgatgtatgt gggcaacgtc
     7441 gccgaagacg gttttgaaaa caatccgcac ctcgaccgcc tgaaagaatt ggcggcaaaa
     7501 gaaaacgccc ccgtcgtcgc cgtttgcgcc gcaatggaaa gcgaaatcgc cgaattggaa
     7561 gacgacgaaa aagccgagtt cctcgccgaa atgggcttgg aagaaccggg cctgaaccgc
     7621 ctgatccgtg ccggctacga ccttttgggg ctgcaaacct actttaccgc cggcgtgaaa
     7681 gaagtccgcg cttggacgat acacaaaggc gacaccgccc cgcaagccgc cggcgtgatt
     7741 cacaccgact ttgagcgcgg cttcatccgc gcccaagtca tttcttacga cgactttgtc
     7801 tcgctcggcg gcgaagccaa agccaaagaa gccggcaaaa tgcgtgtgga aggtaaggaa
     7861 tacgtcgtgc aggatgggga tgtgatgcac tttttgttta acgtgtaacc caaatgcggc
     7921 aggttttcag acggctttgc cagaaatgcc gtctgaagcc ggttttggtg gttttcggcg
     7981 tttccatacc gccggaatgc ggctgcatca aaataaaatc ccgtccgcat ttccgatttg
     8041 ccctccccga ttcctgcaaa acaaaccgcc tgccccgctg ttgcaaaaat ccgtccggca
     8101 ttccgaatat cccgagcccc gatacaaaat gacctttcag atggcatttg cgccgccgcg
     8161 atgaaagcaa aaatattatg agccaagcct taccctaccg cccggacatc gacacattgc
     8221 gcgccgccgc cgttttgtcc gtcatcgtgt tccatatcga aaaaaattgg ctgccgggcg
     8281 gatttctcgg tgtcgatata ttctttgtga tttcaggctt tttgatgacg gcgatcctcc
     8341 ttcgcgaaat gtcggggggg gtttccctga aggcatttta tatccgccgc atcaaacgga
     8401 ttttgcccgc atttttcgcc gtattggcgg caacgctggc aggcggcttc tttttattca
     8461 ccaaagatga tttctttctt ttgtggaaat ccgcgctgac cgccttgggt ttcgcctcca
     8521 acctgtattt tgcaaggggg aaggattatt tcgatcccgc gcaggaagaa aagcccttgc
     8581 tgcacatttg gtctttgtcg gtcgaagaac aattttactt tgtctttccg atattgctgc
     8641 tgcttgtcgc ccgcaaaagc ccgcgcgtac agttcggctt cctcgccgca ttgtgcgcct
     8701 taagccttgc cgcttccttt atgccttccg cgttcgataa atattacctg ccccacctgc
     8761 gcgcctgcga aatgctggtc ggatcgctga ccgccgtgtg gatgcggtat cggcaacagc
     8821 ggaatctcgc cgtcggcaga tgctgtgccg ccgccggcgc attgttttcc gcgtgcatac
     8881 tgtccgcctg cctgtttgcc tattcggaac aaaccgccta tttcccaggg cccgccgctt
     8941 tgattccctg tctggctgtt gccgcgctga tttatttcaa tcattacgaa cacccgctta
     9001 aaaaattttt ccaatggaaa atcaccgttg ccgccggttt gatttcctat tcgctttatc
     9061 tgtggcattg gccgatattg gcctttatgc gctatatcgg cccggacaac ctgccgcctt
     9121 attcgccggc ggcggcggtc gtcctgatat tgctgctttc cctgttttct taccactgca
     9181 tcgaaaaacc gtttaaaaaa tggcaaggtt cgttcgcaca atccgtttta tggatttatg
     9241 ccttgcctat gctcattttg ggggcgggct cattttttgc gatgaggctg ccgtttatgg
     9301 cgcaatacga ccgcttgggg ctgacgcgtt ccaacacctc ctgccacaac aataccggca
     9361 aacaatgcct atggggggat acggaaaaac agccggaact gctggttttg ggcgactccc
     9421 acgccgacca ttacaaaaca ttcttcgatg ccgtgggcaa aaaagaaaaa tgggctgcca
     9481 ctatggtttc cgccgacgct tgcgcctatg tggaaggcta cgcgtcccgc gtattccaaa
     9541 actgggccgc ctgccgcgcc gtttaccgct atgccgaaga acacctgccc cggtattcaa
     9601 aagtggtttt ggcgatgcgc tggggcagcc aaatgcccga aaacagccgc tcccttgcct
     9661 atgatgccgg ttttttccaa aaattcgacc gtatgctgca caaactctca tccgaaaaac
     9721 aagccgttta cctgatggcg gacaacttgg cctcgtctta caacgtccag cgcgcctata
     9781 tcttgtcttc ccgcataccg ggttaccgcc aagccctgcg cccggacgac gaaagcaccc
     9841 tgaaagccaa tgcacgcatc agggaattgg cagccaaata ccccaacgtc tatattattg
     9901 atgccgccgc ctatatcccc gcagattttc aaatcggcgg attgccggtt tactcggaca
     9961 cagaccacat caacccttac ggcggcacgg aattggcaaa gcgtttttcc gaaaaacaac
    10021 ggtttctcaa tacgcgccat aaccattgat tcgcttaaat ttgttacaat cggcggtttg
    10081 caaaaacgct aatttttttg aaagagaccg atgagcgtca tccaagacct gcaatcgcgc
    10141 ggcctaatcg cgcaaacgac cgacatcgaa gccttagacg ctttgttgaa cgaacaaaaa
    10201 atcgcccttt actgcggttt cgatccgacc gccgacagcc tgcacatcgg acacctgttg
    10261 cccgtattgg cattgcgccg cttccaacag gcggggcata cgccgattgc tttggtgggc
    10321 ggcgcgaccg gtatgatcgg cgaccccagc ttcaaagccg ccgaacgcag cttgaattcc
    10381 gccgaaactg ttgccggctg ggtggaaagt atccgcaacc aattaacccc tttcttgagc
    10441 tttgaaggcg gaaatgccgc cattatggcg aacaatgccg actggttcgg cagcatgaac
    10501 tgcctcgact tcctgcgcga catcggcaag catttctccg tcaacgccat gctgaacaaa
    10561 gaatccgtca aacagcgcat cgaccgcgac ggcgcaggca tttccttcac cgagttcgcc
    10621 tattccctgc tgcaaggtta cgacttcgcc gagttgaaca aacgccacgg cgcggttttg
    10681 gaaatcggcg gctccgacca atggggtaac atcactgccg gtatcgacct gacccgccgc
    10741 ctgtaccaaa aacaagtgtt cggactgact ctgcctttgg ttaccaaatc agacggcacc
    10801 aaattcggca aaaccgaagg cggcgcggtg tggctgaacg cgaaaaaaac ctcgccctat
    10861 cagttctacc aattctggct gaaagtcgcc gatgccgatg tgtataaatt cctgaaatac
    10921 tttaccttcc tgtccatcga agaaatcgac gctatcgaag cgaaagataa agccagcggc
    10981 agcaagcccg aagcgcaacg catcctcgcc gaagaaatga cccgcctgat tcacggcgaa
    11041 gaagcccttg ccgccgcgca gcgcatttcc gaaagcctgt ttgccgaaga ccaaagcagc
    11101 ctgaccgaaa gcgacttcga gcagctcgcc ctcgacggcc tgcctgcatt tgaagtttca
    11161 gacggcatca atgtcgtcga agccttggtc aaaaccggtt tggcatcctc caataaagaa
    11221 gcgcgcggct ttgtgaacag caaagcggtt ttgctcaacg gcaaacctgc cgaagccaac
    11281 aaccccaacc acgccgccga acgccccgac gatgcctgcc tgctgaacgg cgaacacaaa
    11341 cgtttcggca aatacaccat ccttcggcgc ggcaaacgca accacgcgct tttggtttgg
    11401 aaataatccg attgccg
//
LOCUS       DAWKQJ010000053        11048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000053 DAWKQJ010000000
VERSION     DAWKQJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.053"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..470)
                     /locus_tag="WJL20_001056"
     CDS             complement(135..470)
                     /locus_tag="WJL20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7683219.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /gene="mtrA"
                     /locus_tag="WJL20_001057"
     CDS             599..1504
                     /gene="mtrA"
                     /locus_tag="WJL20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ7683220.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="WJL20_001058"
     CDS             complement(1566..2054)
                     /locus_tag="WJL20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7683221.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="WJL20_001059"
     CDS             2201..3103
                     /locus_tag="WJL20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7683222.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="WJL20_001060"
     CDS             complement(3144..4274)
                     /locus_tag="WJL20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7683223.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIARRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4479..7103
                     /gene="alaS"
                     /locus_tag="WJL20_001061"
     CDS             4479..7103
                     /gene="alaS"
                     /locus_tag="WJL20_001061"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ7683224.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     TDLVKEMGDAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSEQA
                     IVLLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLASVESWLCQKLS"
     gene            7165..7896
                     /locus_tag="WJL20_001062"
     CDS             7165..7896
                     /locus_tag="WJL20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683225.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8205..>11048
                     /locus_tag="WJL20_001063"
     CDS             8205..>11048
                     /locus_tag="WJL20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7683226.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGAENQWQFPLEELPEYITRGVFDFMFGIEAKVRQI
                     QEEANIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LENCKEADTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRNISPEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLE
                     NLRAEFEAVKALKRDKAKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDII
                     AEALKDKAGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDG
                     EVACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHI
                     KGYGLLDYVCNWYVKAAGIMAQNPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEINF
                     AHRTFQWTSQAAGKAAVHCIIVGFSRKPQTSSENGGANASENSALPKYLYDYPDIKGK
                     PEKHVVANINPYLIDAPDLIIAKRRTQISGENEMCKGNQPTDGGNLILSTDEKEALIA
                     AEPLAEQYIRPFIGAEEFINGKPRWCLWFHGVSDVKRNHDLKQMPKVQARIQAVKAMR
                     EASSKVATQKKAATPWLFDEIRQPSDGNYLIIPRVSSESRRFVPIGYLSHQIIISDSS
                     FMLPNATLYHFGILCSTMHNAFMRTVAGRLKSDYRYSNTVVYNNFPFPESCRMPSESD
                     RPDPLRTAVEAAAQAVLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALD
                     KAVDKAYGYKTGKNTDDEAERTAFLFELYRKAAA"
BASE COUNT         2846 a         3052 c         2608 g         2542 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa aacggctgta tatgttgtct
       61 gcaaaatatc gggaagcata acagacaaaa tgccgtctga aatattttca gacggcattt
      121 caatccgatt cggattattt ttgcgtttca accgcttcca atgcacgcag ggcgtaagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgcctgcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc agcggcggcg tgaacgctga tgcagctctc gcaacgagtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catcaagtgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtaggcctgc agcattttag gatgcgcctt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt tatggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gactggtaca
      721 cattgttaca tcgggcagcg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat gcggcagcac ggtgcgttta cggtcaaaca
      901 gtgcggcaac ggacaggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aacgggctgc ctgaaaccgt ttttctgaac attgcccatc cgagtttaca
     1021 gtatgtggtt tcaatgctgc aactggaaag caaaaaacct ttgacgggga cggtttccat
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 ggattcggtt ttgtcggtcg cactgtcggt aggctttcag tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacag catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcac tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaacccggc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcgtg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgccc tgtctgccgc attttttctg ggcgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggtatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgccggc
     3301 ttgctcgaag gcgcataagt aacgaaattg ccgtttttcg ccgacacttc cggatcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacgccg tctggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagcag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggataaattt ccgccgcgct gtctaaataa
     3721 ctgataccgc attgtttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtcgggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcagggtt gagatgttta taattgggaa tcagtgactt atcgattttc
     3961 tgataagccc ccgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt tgccggtcag caccttgctt tccagtgttt cgtcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt tggttttccg ccggtgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggtc attatagccg
     4321 atattggcaa cagggcttca gacggcattc aaaatcccgc cacacccttc cgaaaaccgc
     4381 cgcttccata gctagaaaca gggatttgcg gtaagatacc gccgttcgtt ttccctgctt
     4441 ttaccatgac aagacatttg agagacattg aaaaaattat gaaaacctcc gaactgcgcc
     4501 aaaaattcct aaaatttttt gaaaccaaag gccacaccgt cgtccgctcc tccagcctcg
     4561 tgccgcacga cgacccgacc ctgctgttta ccaacgcggg catgaaccaa tttaaagacg
     4621 tatttttagg tttcgacaaa cgcccgtaca gccgcgccac caccgcgcaa aaatgcgtac
     4681 gcgcaggcgg caaacacaac gacttggaaa acgtcggcta caccgcccgc caccacacct
     4741 tctttgaaat gatgggcaac ttctccttcg gcgactactt caaacgcgac gccatccact
     4801 tcgcttggga atttctgact tcccccgaat ggctcaacat ccctaaagac aaactgttgg
     4861 cgaccgttta cgcggaagac gacgaagcct acaacatctg gttgaacgaa atcggtatgc
     4921 cgtccgagcg catcgtccgc atcggcgaca acaaaggcgc gaaatacgca tccgacaact
     4981 tctggcaaat gggcgacacc ggcccttgcg gcccctgctc cgaaattttc tacgaccacg
     5041 gcgaagaaat ctggggcggc attcccggca gccccgaaga agacggcgac cgctggatcg
     5101 aaatttggaa ctgcgtattt atgcagttca accgcgacga acaaggcaat atgaatccgc
     5161 tgcccaagcc gtccgtcgat accggcatgg gtttggagcg catggcggca gtaatgcaac
     5221 acgtccacag caactacgaa atcgacttgt tccaagacct gctcaaagcc gttgcccgcg
     5281 aaaccggcgc agctttcagc atggatgaac ccagcctgaa agtcatcgcc gaccacatcc
     5341 gctcctgctc cttcctgatt gcagacggcg tattgccgtc caacgaaggc cgcggctacg
     5401 tattgcgccg cattatccgc cgcgccgtgc gccacggtta caaactgggt caaagcaaac
     5461 cgttcttcca caaactcgtt accgatttgg tcaaagagat gggcgatgcc taccctgaat
     5521 tgaaagaaaa acaggcgcaa atcgaagaag cgttgaaaaa cgaagaaagc cgttttgccc
     5581 aaactttgga aaccggtatg gctttgttgg aaaatgcact ggccaaaggc ggcaaaacac
     5641 tcgacggcga aatcatcttc aaactctacg atacttacgg tttcccatac gacctgaccg
     5701 ccgacatctg ccgtgaacgc aatattgaac tggacgaagc aggcttcgag cgcgaaatgg
     5761 aagcccaacg cgcacgcgca cgcgccgccc aaagcttcaa agccaacgcc caactgcctt
     5821 atgacggtca agacaccgag tttaaaggtt atagcgaacg ccaaaccgaa tccaaagtcc
     5881 tcgccctcta caaagacggc gagcaagtca acgaattgaa cgaaggcgac agcggcgcag
     5941 tcgttatcga ctttaccccg ttctatgcag aatccggcgg ccaagtcggc gacgtcggct
     6001 atatcttctc aggcgaaaac cgctttgaag tacgcgatac ccaaaaaatc aaagcggccg
     6061 tattcggtca attcggcgta caaacttcag gccgtctgaa agtcggcgac agcgttaccg
     6121 ccaaagtgga tgacgaaatc cgcaatgcca atatgcgcaa ccacagcgca acccacttga
     6181 tgcacaaagc cctgcgcgat gtattgggcg aacacgttga acaaaaaggc tcgttggtta
     6241 ccgccgaatc cacccgtttc gacatttccc atcctcaagc ggtaactgcc gaagaaatcg
     6301 ccgaagtaga acgccgcgtc aacgaagcca ttttggcgaa cgttgccgtc aacgccgcca
     6361 ttatgagcat ggaagacgcc caaaaaaccg gcgcgatgat gcttttcggc gaaaaatacg
     6421 gcgacgaagt acgcgtactg caaatgggcg gtttctctac cgaattgtgc ggcggcacac
     6481 acgtttcacg caccggcgac atcggcctct tcaaaatcat cagcgaaggc ggtattgccg
     6541 caggcgtgcg ccgtatcgaa gccatcaccg gcctgaacgc actcaaatgg gcgcaagagc
     6601 aagagcgttt ggtgaaagac attattgccg aaaccaaagc ccaaaccgaa aaagacgtac
     6661 tggcaaaaat ccaagcaggc gcggcacacg ccaaagcatt ggaaaaagaa ttggcacgcg
     6721 ccaaagccga actcgccgtc cacgcaggcg ccaaactctt ggacgatgca aaagacttgg
     6781 gcgcagccaa actcgttgcc gcccaaatcg aagccgacgc agccgccctg cgcgaaatcg
     6841 taaccgacct gaccggcaaa tcagaacaag ccatcgtatt gctcgccgca gtcaacgagg
     6901 gcaaagtttc cctgtgcgca ggcgtatcca aagccctgac cggcaaagtg aaagcaggcg
     6961 atctggtcaa atttgcagcc gaacaagtcg gcggcaaagg aggcggccgt cccgacttgg
     7021 cgcaggccgg cggcacggat gccggcaaat tgcctgaaat gctggcaagc gtggaaagct
     7081 ggctttgtca gaaattaagt taaatttaaa tcttaataag ccatttgagt cttgttcaaa
     7141 tggcttatat tttggattgt tagaatgaat actaaaacag agttacaaaa acttttagaa
     7201 gaggatatat caactttaac agaaacacta atttgtgcag atgctcttcc tccaagatat
     7261 gtacgtagca ttgcaactcc tattgtccgt cgatggttaa ttgacaaaca attaaatatt
     7321 ctcgcaaaag aaataggttt aactatagaa ttgcccatcc ttgatacaag tttggtattt
     7381 gaaaaattgt caaccttaga gaataaagtt aatttttata tggctggtgg agtttattta
     7441 ggcggagaat ttattagctc tatatatcat tcatcacaag aattttctgg tgaaccaata
     7501 atctatgctg aaccaaatat aattttatgt cctgctgaaa aatttttaac tttaaaacga
     7561 gttttccata atggaaatat ttttaatatg aatcagataa ttactttttt atccaataaa
     7621 caaggaggag tacattttga taaaaattac gataaatata aaacttggca agttgctata
     7681 gaaaaagcag caaatttttt aaaactcggt aatccatata atgaagataa attatctctt
     7741 agtgaagaac atgatactat tttagtagtt ttaccattag aaaaaggata tgaatggaat
     7801 tgtcttgaga tagaagtact atccgctgct caatctctag caaatatcta ttgcaacaaa
     7861 gttcgcctca tagatggtca tgtttggaag gaataaaaga aaaagcatag ttggtaattt
     7921 ttagttacgt agggtgggct tcagcccacc gttttttcat ttttgattgt tgattggtgg
     7981 gctaaagccc accctacggt tactaaaatc ccgtcattac ctcgcaggcg ggaatccggt
     8041 ttgttcggtt tcggtttttt agggacagtg ccgcaacatt aaatttccag attcccactt
     8101 tcgcacaaat aacagcgcaa atagggcaaa gataaggata tggggggttt tttctttgaa
     8161 tttactatac aattcgcccg tccccgttat agaaacccaa gccgatgaaa accctgctcc
     8221 aactccaaac cgccgcacaa aacttcgccg cctactacaa agaccaaacc gacgaacgcc
     8281 gcgagaaaga caccttctgg aacgaatttt tcgccatttt cggcatcgac cgcaaaaacg
     8341 tcgcccactt cgaatacccc gtcaaagacc ctgccgacaa cacccaattc gtcgatatat
     8401 tttgggaagg catcttcctt gccgaacaca aatccgccaa caaaaacctg accaaggcca
     8461 aagagcaggc ggagcgttat ttgcaggaaa tcgggcgcac caagccctcc gcgctgcccg
     8521 aatattacgc cgtcagcgat tttgcccatt tccaccttta ccgccgcgta cctgaagaag
     8581 gcgcagaaaa ccaatggcag ttccctttgg aagaactacc cgaatacatc acgcgcggcg
     8641 ttttcgactt catgttcggc atcgaagcca aagtccgcca aattcaagaa gaagccaaca
     8701 ttcaagcggc ggcggccatc ggcaggctgc acgacgcgct caaagaagaa ggcatttacg
     8761 aagaacacga gctgcgcctc ttcatcacgc gcctgctttt cctctttttt gccgacgaca
     8821 gcgccgtttt ccagcgcaac taccttttcc aagacttttt agaaaactgc aaagaagccg
     8881 acacgctcgg cgacaagctc aaccaactct ttgaatttct caacacaccc gaccaaaagc
     8941 gcagcaagac ccaaagcgaa aaatttaaag gtttcgaata cgtcaacggc ggtctgttta
     9001 aagaacgcct gcgcactttc gacttcaccg ccaagcagca ccgcgcctta atcgactgcg
     9061 gcaatttcga ctggcgcaac atcagtccag aaatcttcgg cacgctcttc caatccgtca
     9121 tggacgcgca agagcggcgc gaagcaggcg cacactacac cgaagccgcc aacatcgaca
     9181 aagtcatcaa cggccttttt ttagaaaacc tgcgtgccga atttgaagcc gtcaaagccc
     9241 tcaaacgcga caaagccaaa aaactcgccg ccttctacca aaaaatccaa aacctgcaat
     9301 tcctcgaccc tgcctgcggc tgcggcaact tccttatcgt tgcctacgac cgcatccgcg
     9361 cccttgaaga cgacatcatc gccgaagccc tcaaagacaa agccggcggc ctgttcgaca
     9421 gcccctccgt ccaatgccgt ctgaaacagt ttcacggcat cgaaatagac gaatttgccg
     9481 tcctcatcgc ccgcaccgcc atgtggctca aaaaccacca atgcaacatc cgcacgcaaa
     9541 tccgcttcga cggcgaagtc gcctgccata cgctgccgct cgaagacgcc gccgaaatca
     9601 tccacgccaa cagcctccgc acaccttggc aggcggcgga ctacatcttc ggcaatccgc
     9661 cctttatcgg ctcgacctac caaaccaaag agcagaaaaa cgacctcgaa agcatctgcg
     9721 gccatatcaa aggctacggc ctgttggatt acgtctgcaa ctggtatgtc aaagcagccg
     9781 gcatcatggc gcagaatccc caagttcaga cggcatttgt ctccaccaat tctatctgcc
     9841 aaggccagca ggtcgaaatc ctttggggca gccttttaaa ccaaggcata gaaatcaact
     9901 ttgcccaccg taccttccaa tggacgagcc aagccgcagg caaagccgcc gtccattgca
     9961 tcatcgtcgg cttcagccgc aaaccgcaaa cgtcgtctga aaacggcggc gcaaacgctt
    10021 cagaaaattc cgccctgccc aaatacctct atgactatcc cgacatcaaa ggcaaacccg
    10081 aaaaacacgt cgtagccaac atcaatcctt atctgattga tgcgcccgat ttgattattg
    10141 ccaagcgcag aacccaaatc agtggggaaa atgaaatgtg taaaggcaac cagccgaccg
    10201 acggcggcaa ccttatcctt tccaccgatg agaaagaagc cttgattgcc gccgaaccat
    10261 tggcggagca atacatccgt ccctttatcg gcgccgagga atttatcaac ggcaaacccc
    10321 gttggtgctt gtggtttcac ggcgtatccg atgttaaacg caaccatgac ttgaaacaga
    10381 tgcctaaagt ccaagcccgt attcaggcgg tcaaagccat gcgcgaagcc agcagcaaag
    10441 tcgccacgca gaaaaaagcg gcgaccccgt ggctcttcga cgaaatccgc cagccttcag
    10501 acggcaatta tctgattatt ccacgcgtat cttctgaaag ccgccgtttc gtccccatcg
    10561 gttatctgtc gcatcaaatt atcatcagcg attcatcttt tatgcttcca aacgccaccc
    10621 tctaccactt cggcatcctc tgctcaacca tgcacaatgc ctttatgcgt accgttgccg
    10681 gccgtctgaa aagcgattac cgctattcca ataccgttgt gtacaacaac ttccccttcc
    10741 ccgaaagctg ccggatgccg tctgaaagcg accgcccaga cccgctccgc accgccgtcg
    10801 aagccgccgc ccaagccgtc ctcgacgcgc gcggacaata ccgccgagaa gcgcgggaag
    10861 ccggtttgcc cgagccgacc ctcgccgaac tctatgcgcc cgacgcaggc tataccgccc
    10921 tagacaaagc ccacgccgcc ctcgacaagg cagtcgataa agcctacggc tacaaaacag
    10981 gcaaaaacac cgacgacgaa gccgaacgca ccgccttcct gttcgagctg taccgcaagg
    11041 cggcggca
//
LOCUS       DAWKQJ010000054        10781 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000054 DAWKQJ010000000
VERSION     DAWKQJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.054"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(141..836)
                     /locus_tag="WJL20_001064"
     CDS             complement(141..836)
                     /locus_tag="WJL20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7683227.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1007..1666)
                     /locus_tag="WJL20_001065"
     CDS             complement(1007..1666)
                     /locus_tag="WJL20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ7683228.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1869..3980)
                     /locus_tag="WJL20_001066"
     CDS             complement(1869..3980)
                     /locus_tag="WJL20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7683229.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDEQNDPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            complement(4166..4771)
                     /locus_tag="WJL20_001067"
     CDS             complement(4166..4771)
                     /locus_tag="WJL20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7683230.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALCAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4820..5218)
                     /locus_tag="WJL20_001068"
     CDS             complement(4820..5218)
                     /locus_tag="WJL20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7683231.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5326..6237
                     /locus_tag="WJL20_001069"
     CDS             5326..6237
                     /locus_tag="WJL20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7683232.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6227..7528
                     /locus_tag="WJL20_001070"
     CDS             6227..7528
                     /locus_tag="WJL20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ7683233.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDGPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(7596..7820)
                     /locus_tag="WJL20_001071"
     CDS             complement(7596..7820)
                     /locus_tag="WJL20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683234.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVAAWVAVIWAYLMIESEKNGRY"
     gene            7923..10073
                     /gene="dinG"
                     /locus_tag="WJL20_001072"
     CDS             7923..10073
                     /gene="dinG"
                     /locus_tag="WJL20_001072"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ7683235.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIARIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            10109..10720
                     /locus_tag="WJL20_001073"
     CDS             10109..10720
                     /locus_tag="WJL20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ7683236.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGQATVTQSDFQPAGETEETYSRTVLRT
                     MIAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAAE
                     AYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2547 a         3074 c         2727 g         2433 t
ORIGIN      
        1 gtactggttt ttgttaatcc gctatatatt ctgatttaaa accataaggc tttaagcaat
       61 catctcttct ataaagccta aatacaaaag gccgtctgaa atcctatttt cagatggcct
      121 ttactcattc aatcctcaac ttattgcggc tttttctgct cttcgcgtac tttatccacc
      181 aactggtcaa tcgtggtcat accggactgc cagtctttaa attcaacttg gtatttgccg
      241 ccgacaatca cagtcggtgt gccgctgatt tggaatttat tggtcaactc ttccatttga
      301 gccgcacgcg cttggctttc aggagcctca aatgcagcca atactttttt gccgtcaaac
      361 gctgtttgct cggacagcca ttttttcagg gtatcggtat cggccagatt gattttttga
      421 ttaaccatcg catcgaaaat atggctgttg gctttatctg attcaccggc catttccact
      481 gcggccgcca aacgtgccaa aggtttcatt tcatcacccc acacgacatg ctcccggcgc
      541 atataggtat cgtctttaaa cgttttgatg tgctcgctca agaccggctc aagatgggcg
      601 caatgcgggc agaagtagcc gaaaaattcc aatacttcga ttttaccggc ctgctgttgc
      661 ggaataggcg tagacaatac agtgtagttc acaccttcgt tcaactcagc aggggctgcc
      721 ggagcagatg agctgctttg gacgctgtct gccggaacgc tggtttcagc ctgtttgcta
      781 caagcggcca atgccaacag ggtcaatgaa gtcaaagcta aggttttcag tttcataggt
      841 atctcttgtg tgtcagatta atgtgcggat tttatgacat tttattgaag gcgtgttcga
      901 tttgagtgaa aaaatgtgtt tagttttatt tcagccttta taaaggccgt ctgaaatatg
      961 gcttagataa ttagccttat tttcagacgg cctttgttct agtaatttac ggtttcggac
     1021 attttgtctt caatccgtcg atgtgttcca ttaagtcagg tgggacatga ttccacatag
     1081 gatcaagaat aaaatcgata ccttcccgtc tggctaattt ggctgctggg acaaaatcgg
     1141 aatcaccaga aaacaagatg attttatcgg cttgtttttt taaggtaata gatgaaatat
     1201 ccaagcctat acgcatatct acaccttttt gtttaacatc gagcacgaca tcgttttccg
     1261 taatttgagt tgacgggttt ttcagaagat ttttcatgac ttggggtttt aatatccatg
     1321 ctgtattgtc cgacagcttg cctaaacgta acgctaattt acgcttcttc ttcaattcgg
     1381 tatgcagatt gcaacgaaat atggcttccg gagtctttga aaaatctatg gcacgatgag
     1441 atacgggatt gtgcattttc ttttcaaacg gcacgcagtc gtaaaagaaa attctatata
     1501 aatcatccat ttgatctgtg ccgttgattt tatctttcag atgcagcatc gaatatcgga
     1561 aagccaaatc agcaattctt tttgcatcgt gtggattttg agacaggcct aatgaccgta
     1621 cccgcttaat aaaaaatgca ccgtcaatca aaatggcggt tttcatattg cttcccctat
     1681 atttgtcaaa gatataaaaa agcccttggg atcggcatac ccgctatgat ttaatgggta
     1741 atgcgtactg ccaagggcgc tatttctttt tggattatac tgatgtagat aaagcctgtc
     1801 aaaaagttcg tgtgctgaat ttacggatta tcaggatttt cagacggcat tctgttgcac
     1861 gaacgggatt agaaactgta attaacccgc gccgtataag tgcgcggcgc gccgggcatg
     1921 gcatccgaac gccaatattt ttgattgagc agattggctg cggcaaaggt aatgttaaca
     1981 tttttatggt tccagccaag catggcatca actcgggcaa agcctggaag cgtagtcact
     2041 tctttatttc ttgagttgta accgtagcgt ttgcctgtac cggttacgcc gatttcgccg
     2101 tagaggtttt cggtcggggt ataacggaaa aacaggttgc cggtaacgtt gctggtgtta
     2161 ttcaaatgga tgcccactcg gtcgggattt tctttgtctt caacgacttt cgcctgcatc
     2221 acgcccaacg aaccgcgcag atagagtttt ttggggatga tttgcccgat ggcggacaat
     2281 tccacgccgc gcgaacggtg tttgccgccg actgcccaag tgtagggatc attttgctcg
     2341 tcggggcggt agcggatatt gaagcgttcg atttggtagg cagacaacgt agtgctgagg
     2401 cggtcgtcca gccaactgct tttcacaccg gtttcgtatt ggcgggtgta ctcggggtcg
     2461 gcgttgaaca cggcggaaga cgacgtgttg atgctcaaat agccgccgcg tccgccataa
     2521 ggcgcgaagc ctttattata cgaggcgtaa agtgtgtgga cgggattgat gttccacact
     2581 gcgccgatgt tggggctgaa cgagtgtccg ctgtattggc ggctgctgcc ggtgagtttg
     2641 ttttcggaat taaaggtgta tttgtcgtaa cgaccgccga ggacgaattt caaatcgggc
     2701 gtggcggaga agatgttttg cacaaagatg ccgtaggagt cggctttgtg gcggttttgg
     2761 gtgaggatag gctgcaatct gcccgaagcc ggccagcttg ctcggtcgta tggatcgatg
     2821 gaagcagtaa aggcgcggct gtaacctaag gtcgggttgc ggtgttcgcg gctgtaatcc
     2881 atgcctacgg tcaagtggtt ttcaaaacgg ccgatggtgt agtcgccgtt tagcgtgaaa
     2941 ttggacgaca gggttttgtt gtcggtctgc tgccaggcgt agttacgttt gattaagttg
     3001 ccattttcgc tgcctgcata gaaatgatca aaatcctgcg ccgccgtgcg gtgggcgagc
     3061 tgccattggg cacgccattt gtcgttgaag gcgtattcaa ggtcggagcg ccaaacttgc
     3121 agcttgtctt tgacaaaatc gttccggtgg gcgaacccca tgcggtaagg cagtccgaag
     3181 cggtcgtaca cggacttggt cggactgcgg tcgggcgtgc gctccacatt gtcgtaggtg
     3241 tattgccccg tccacttcaa gccgttgtcg agtttgacgg taatgctggg cgaaaccatg
     3301 acatttttgc tgtctatgcc gctgcggaac gaattggcgc gcccgacttc gccggtgaga
     3361 cggatggcga cgtttttgtt caacacttcg ttaatgtcca tattcaggct gcggtttgcc
     3421 cacgaaccgt aaaccgcacc gatgttgcgg ctttgtttga agttggcgta tttgctgacc
     3481 atgttgatga cgccgccgcc gttggtgcgg ccgtaaagca cggaagacgg gcctttcagg
     3541 atttccacgc gctcgatgtt ggcagtactg cggcgcactt gtccgctttc gcgcacgccg
     3601 tcgcggtaaa tatcggatgc gtcggcttga aaaccgcgca ggaaaatgct ttcgccgcgc
     3661 atatcgtagg cagcgtcgat gccggcattg ccttcgagga tggaactcaa atcgttcgta
     3721 ccgtaatttt tgtttttctg gatattgagc gtatcgatgg tttgcggcgt ttctttgatg
     3781 agctgtccgt tgcgggtaac ggcggcttcg tcgtagttga tgtagccttt gagtacgctg
     3841 gtgtcggact gtccgaccac ggaaacggtg ggcagagtgg cggtgtaatg ttcaccattg
     3901 tcctgcgtat cggcggcagc aacagggaag gaagcaataa tcagcgtggg taataaagct
     3961 aaatgaaatg atattttcat ttttatactc aatttaacaa aacaaccgaa ttatattgcc
     4021 tcacggagga aatgagaata atttctttta actatattga acatgatatt tgtaaacaaa
     4081 ggtctcagaa tgcggaaaac tcgccgcctg atactgaaaa atgccgtctg aacagggttc
     4141 agacggcatt tttttgaccg cgaaattatg cgccgaacac tttcaaacgt tctgcaactg
     4201 gttcaaaggt cttttcacct gccgcccctt caataccgcc gataaccatt tgtgcgcgca
     4261 acaaccagtt ttcggggata ttccacgctt tggcaatcgc cgcatcgggc aagggattgt
     4321 aatgttgcag gtttgcacct acgccgaccg cggcaagtgt cgtccaaacg gcatactgca
     4381 ccatcgcgtt cgcctggtcc gcccaaacgg gaaagttggc ggcataagca gggaactgct
     4441 cctgcaaacc tttgacgaca ttttgatctt cataaaacaa aatagttgcc gcacccgcct
     4501 taaacaggtt caatttttgc gcggtcggtt caaaactgtc ggcaggcacg acggcacaca
     4561 gcgcgtcttc gacaaattgc cacaccttat catgctcttc gccaaacagc acgaccacac
     4621 gggcagattg ggaattgaac gaagaaggtg tgtgcaaaac ggcgtgttcg acgatttgga
     4681 cgatttcatc tttgccgacg ggcagatttt tatttaacga ataaatggaa cggcggcttt
     4741 cggcagcctg ttgcagagat tgacgggtca tttcttttcc tcaaaaacat tcaacaaaat
     4801 acactttagg cagtccgatt caggctttac gcttgaaaaa cagcctgtcc agcgaccatg
     4861 ctccgccgcc cgccgccgcg atatagagga atacgaagca gaacagcact gcggactcgc
     4921 cgccgttggc aatcgggaac aaagcatttc cggaagcgtg cgccataaaa taggcaaccg
     4981 ccatctggcc ggacaaaaca aacgcggcag ggcgcgcaaa caggcccaac accagcaaaa
     5041 tgccgccgac aatttctaaa ataccggcaa gcagcaacag cccgccgggc gaaccgctgc
     5101 ccatttcaat ggggaaggcg aagattttcg acgtaccgtg caacaaaaac aggtaggcgg
     5161 ttacgatacg caaaacagaa agcaaaaccg gttggatacg gttgcagcaa tcggacataa
     5221 aagctccttt gatatttgtc ttcatttcag gcggtagagt atagatgttt ccccttttca
     5281 aatatatact gcttttattg atacaacttt tccacacaag aaaatatgga caccctgttc
     5341 agcctcaagg ttttccgcca agtcgtccaa agcggcggct tcacccgcgc cgccgacgcg
     5401 ctcggcatct ccaccgcaat ggcaagcaaa cacgtcagcc acttggaaaa caccgtccaa
     5461 gccaaactcc tgcaccgcaa cagccgcaac ctcagcctga ccggagccgg ggaagaatac
     5521 taccggcaat gcagttacgc gctcgacacg ctcgacgatg ccgcgcaaaa agccgccggg
     5581 gggacggaaa aaccgcaggg gctgctgcgc gtaacgatgc cgctgtggtt tgccggcagc
     5641 cagatatgca actggctggc ggaataccgc gaacgttatc ccgaagtggc attagaactg
     5701 attttggaca accgccacgt cgatttgatt gccgaaggcg tggatttggc gttgcgcgtt
     5761 tcccaaaccc tgtccccgtc actgattgcg cgcccactgg cggaaatcga atttgccctg
     5821 cttgcctcgc ccgacttcct gaggcgcaac ggcgtgccgg aaacgccgga agaagtggcg
     5881 gggctgcccg ccgtcctgcc gacctacacc aaccagcaga aactcgacct cacccgcaaa
     5941 tcggacggca aaaaataccg gcttgaactg acccccgtca tccgtaccga caacacgctg
     6001 atgatgcgcg aaatgattaa ggcgggcgcg tgcatcggtt atcagccgct ttgggcggcg
     6061 gaacacgatt tgcgctgcgg cacgctggtg aggctgctgc ccggatacgc cgtcccgacc
     6121 gaccggctga atgccgttta tgcagacagg gcattcttaa gcgcgaaagt ccgcagcttc
     6181 atcgattttc tgaacgaaaa aatcgccagc aggaaaggct gccgaaatgc cgtctgaaac
     6241 cgcccgcccc ctttatgcgg acacgtgcgc cgcacacacg ctcgtctggt tccgccaaaa
     6301 cctccgcatc cgcgacaacg ccgccttatg caccgccgtt gccgaaggtt tgcccgttat
     6361 cggtatttgg attgacgatg ccgaaacaga caaccctcgc cgcgccgcgt tctaccgcca
     6421 atccgccgcc gaactcgccc aagggcttgc agggcgcggc atcccgctct acacggcggc
     6481 atcgcctgcc gagctcgtcc ggctcgccgt ccgcctcaat atccgcgccg tcatcgccga
     6541 cgaatcccat acttttgccg acaaactcgc cgacaacgcc ctttggcatg aattggacaa
     6601 acacggcatc gcgttaacct tcgtcaatga ccgcgccgtt ttcggcaaaa ccgacctgat
     6661 acccgacggc ggcacggcac acaccgattt cgaccgctac cgcgaagtat ggctcgaccg
     6721 cttttccaag cagccccccg ccggtccgaa cctattcgcg gcataccgcc aacccttccc
     6781 cgaaaacctt tccgccccgc cgcctgccgc cctttcagac ggcatcttcc tgccgcaaaa
     6841 cagcggcgaa acggcggctt ggcggcagtg gcggcggttt ctcgaacagg cggattccta
     6901 ctccgtttta aaggatttcc cctcgcgcaa acacacttcg ctgatgggcg cgtatttgag
     6961 tgccggctgc atctcgccgc gcctgctcgc gcgggaaagc ctcgaacgcc gtctgaacgc
     7021 gtgggcggac aacatcatcc gccgcgattt tttccttcaa cttgccttgc agcacacgga
     7081 tgacggccct tcagacggca atccggaaca caccctgcgc ctgacgcttt ggcagcaggg
     7141 ccagaccggc attccgatta tcgatgccgc gatgcgctgt ttgcacaaaa ccggcagcct
     7201 ccaccccgcc ctgagacgct tgagcgcgga ttttttctgc cacgttttaa acctcccccg
     7261 ccgcgaaggc gagatatggt ttgcccggca gctgaccgat ttcgatgcgg caatcaacca
     7321 aggcaactgg tggcttgccg cctcacggca cacctgcccc gacattgccg ccgcctcata
     7381 ccgtaccgac ccggacggta cttttatcaa acggcacatt cccgaactcg cccacctgtc
     7441 cgccgacacc gtccacacgc cttggcggtt tgcctgttcg gtcgataccc acggctatcc
     7501 cgcctatcct gtcgccggta ttccctaagc ggtacggcaa cctaaaacaa atgccgtctg
     7561 aacttccgtt tcagacggca ttgcgtccga atgactcaat atcttccgtt tttttcactt
     7621 tcaatcatca ggtatgccca aatcaccgcc acccaagccg ccaccgttcc catcgccggc
     7681 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
     7741 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
     7801 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
     7861 gatattgtac cgcttccgtg caatgttaaa ataagcccac ttctaccgcc gtacaaaacg
     7921 ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaac atcggcaaaa
     7981 acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat gattgcggcg gttgccaacg
     8041 ctttttcgcg gacgttggcg cgcgaagaag gcggcgagcc gcccaagcgc gagggcgaga
     8101 gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc gcttgcctac ctgctggcgg
     8161 gcggcatcat ggcgcagacg cgcggcaagc ggctgattgt gagcagcgca acggttgcct
     8221 tgcaggagca gttggtagac cgcgacctgc cgtttctggt cgaaaaaagc ggtttggaac
     8281 tgaccttcgc acttgccaaa gggcgcggcc gctatctctg cccctacaaa ctctatcaac
     8341 tgacgcaaag caatgcccag caaaacctgc tcggctttga agcccccgcc gtcttgtggg
     8401 acagcaaacc caagcccgaa gaattgaagc tgctgcgcga catcgccgac gaattttccg
     8461 cccgacggtt caacggcgac cgcgacactt ggccggaaaa aatcgatgac gcgatttggc
     8521 tcaaagtgac caacgaccgc cacggctgcc tgaaaaccgc ctgtcccaac cgtccggaat
     8581 gtccgtttta cctagcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt gccaaccacg
     8641 atcttctgct tgccgacatc agtatgggcg gcggcgtgat tctgcctgcg cccgaaaaca
     8701 gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc cgttttgccg
     8761 ccgaacattc atggaatatt gccgtttgga cgctggaaaa actgccgcag ctgaccggca
     8821 aaattgccgc gcttaccgat aaagccgaac ttgccaacct tgccgacgaa gccgcctcat
     8881 ccttactcga cagcctgcac gaatggcagt tccatttggc ggaagagccg tctttgagct
     8941 tggggccgtc tgaaaacgac aggcggacca acagcgaacc gacttggctg tgggaagacg
     9001 gtaaaatccc cgaaggcctc gaaaccaccg tttccaatac ggccattgct gcgcgcagcc
     9061 tgctcaaaca cgttgtcggg ctgaacgatg cgctttccgc cgcgcgccgc gaaaaagaac
     9121 aggacggcgc gctcctcgac cgcctgaccg gcgagttcgg catcttcatc gcccgtatcg
     9181 aacaaatcag cgcggtttgg gatttgctct ctaccgttcc cattgagggc gaagagccgt
     9241 tggcgaaatg gataacccgc cgcgccgacg acaaaaacga ctacattttc aacgccagcc
     9301 ccatcagcag cgcgtcccac ctcgccaaca gcctgtggcg gcgtgcggca ggcgcggtgt
     9361 tgacctccgc caccctgcaa tccttgggca actttaacct gatgctgcgc caaaccgggc
     9421 tgcaatggct gcctgaaacc accaccctcg ccctcaaaag cccctttgac tttgaaaaac
     9481 agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa gcacacaccg
     9541 ccgccgtcat cgagtggctg cccaagctta tttcgcccac cgaagccatc ggcacactcg
     9601 tcctgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc ggagactacc
     9661 tgccgctcct gctcgtacaa ggcgaattgc ccaaagccgt cctcctgcaa aaacaccacc
     9721 gggccataga agaaggcaaa gccagcatca tcttcggact cgacagcttt gccgaaggac
     9781 tcgacctgcc cggcaccgcc tgcgtgcaag tcattatcgc caaactcccc ttcgccatgc
     9841 ccgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggc ggcaacccct
     9901 tcatcgaaat caccgtcccc gaagccggca tcaaactcat ccaggctgtc ggccgcctca
     9961 tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcatc aaaacacagc
    10021 ggtacggcca acaattatta gccggcctgc caccgtttaa aaggataggg taaacctacc
    10081 gcccttcccg caacagaaag gaaaagtcat gaacttcacc cgactgctca accaagtctt
    10141 aagcacggtt caaaaaaaag gcaacacatt ctccgacagc ccgctcaatt cattcggcgg
    10201 aggcgcgctg gttgccggtg tcgcctccat gctactgaac ggtaaaaacc gcaaaaccat
    10261 caccaaaatc ggttcgaccg ccgctttggg ctacctcgcc taccggggct atcagatgtg
    10321 gcagcaaaac aaagggcagg caaccgtaac acaaagcgat ttccaacctg ccggagaaac
    10381 tgaagaaaca tacagccgca ccgtattgcg caccatgata gccgccgctt cagacggcat
    10441 gatagacgaa gccgaacgcc ggactatcga acaggaaagc ggcacagacc ccgaaactgc
    10501 cgcatggctc gccgccgaat accgcctgcc cgcaagcatc ggggacatcg ccaccgccgt
    10561 cggcaacgat gaggcgttgg cggcggaggc ctatctggcg gcaaggttgg tctgtgccga
    10621 tttgtcgcgg aaagaaaccg tcttcctcgc ccgcctgtcg caggctttga aactggatga
    10681 caatctggtg gagagtttgg aaaggcaatt ggggttttga tgacacagct ggggagggaa
    10741 aatcatcaaa aaacggttat tccgaccccg gcctcaaaac g
//
LOCUS       DAWKQJ010000055        10759 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000055 DAWKQJ010000000
VERSION     DAWKQJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.055"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(191..2599)
                     /locus_tag="WJL20_001074"
     CDS             complement(191..2599)
                     /locus_tag="WJL20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ7683237.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2669..3673
                     /locus_tag="WJL20_001075"
     CDS             2669..3673
                     /locus_tag="WJL20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ7683238.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3730..4428)
                     /locus_tag="WJL20_001076"
     CDS             complement(3730..4428)
                     /locus_tag="WJL20_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7683239.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(4722..4829)
                     /locus_tag="WJL20_001077"
                     /pseudo
     CDS             complement(4722..4829)
                     /locus_tag="WJL20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4887..6425
                     /gene="murJ"
                     /locus_tag="WJL20_001078"
     CDS             4887..6425
                     /gene="murJ"
                     /locus_tag="WJL20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ7683240.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFFLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            6558..7514
                     /locus_tag="WJL20_001079"
     CDS             6558..7514
                     /locus_tag="WJL20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ7683241.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQASA
                     AISAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7565..8347
                     /gene="trpC"
                     /locus_tag="WJL20_001080"
     CDS             7565..8347
                     /gene="trpC"
                     /locus_tag="WJL20_001080"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ7683242.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRAGDIEAEVGKLF"
     gene            8412..10712
                     /gene="recQ"
                     /locus_tag="WJL20_001081"
     CDS             8412..10712
                     /gene="recQ"
                     /locus_tag="WJL20_001081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ7683243.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKIDRFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQATAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLTVLGQNAV"
BASE COUNT         2202 a         3046 c         3081 g         2430 t
ORIGIN      
        1 gatgcggttt ttgccttttg cggtgcggcg tggacatcgg ctgctgccag caatgcggcg
       61 gcaatcatca gggctttgat tttcatcatt ctttccttat cgtttgaatc ctacctttga
      121 tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg ggaacggtgc tcctgccggg
      181 tttccgacgg tcagggtcgt ttgttgcgtc cggcggaaag agagtgggcg gggatgtagc
      241 cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc gacactgctg aggtctttca
      301 actgaagtga gaaaaagaca gcgtttttgt aggtgttttc gccggtaacg tagcgttggg
      361 cgtacacgcc cgcgccccag cagccgcaac tgcttttgta ttccgcaccc gccagcacct
      421 ctatcggttt tttggcttca aaaccgtagt tgtaacggac gacggccgac aggttgcgcg
      481 tcagcggcca ttgtgcggac aggtcgagct ggctgagttt gtcgtaaaaa taggaaccgt
      541 cggacttcag gtagattttt tcgttgcgcc cgtatttgta gcgggcgttc agcactttgc
      601 cctgtgcggg acggtagctt gcaccgacgg cgtagttctc ggcgcgtttg tcgttttggt
      661 tgtagtggat gctgctgtcg aggatgaagc ggctgccgat gctgccggag gcaaatgcca
      721 cccagtcgga acggttgcgc ggttttttgc cgacgctgcc gtcaagcatc accgcatcat
      781 ccttgaaata gaatttctga ccgatgccgg cgcggaaacg ctcttccccc gtcgcgccgt
      841 ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc ggtgttaatc ctgtcgttgc
      901 cgtaatagag gttttcgcga aagagctgcc cgtagccgaa gctgctttcc gacgaatcga
      961 aattgggcag gtcgttttgg gatttggcag gaatatagtt gtagaacagg cgcggctcga
     1021 gggtttgcag gactccgccg ccgaacatcc gcgtattccg ctcaaaagtt gcgccgctgt
     1081 cgatgttgac aatgggcaga gtgcggctga cgcgtcgggc ttcttggctg ccgaagcggt
     1141 tgaggctgta ataggtggcg tgcagtccga gtttgggacg gacgtaaccc cagctgttgc
     1201 tgaaatccca tttgatgtcg ggatagacga cgaggcggct gccgtcttgg cggctgtcgt
     1261 ggctgaagcg ggtaaattgt gcggacacgc cgatttgcgc cctgccggtg tttttgcgcc
     1321 aatcggcgga aaggcgcggc atcagggcat acggtttgtc tttgtagccg ctttggtttg
     1381 ccagcgtctg gtatttcaga accgaaaggc cggcattcag gctgccgccc gccgccctgc
     1441 cgccataatc cagccataca cggcggttga ggttgacgtt gccggcgatt tctttgttgc
     1501 cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt gaaatcgaca cccgcctgaa
     1561 gcgtgtcgga aatgtcgtgc cgatgctgcc atttcgcctg atagcggtta ttcctgccgc
     1621 ttttcttgtc gtgcggcagc caggtcaggt cggactggcc ggcataatcc ggccgcaggt
     1681 agcgtacctg cccgtcaaag accgcgccgc gttcgccgat cacgctgggc gcgaacgtgg
     1741 catcgagatt gggggcaagg ttgaaataat agggaacgga aagggaaacg ccgtccgaac
     1801 cggcggacag tgagggaaca agcaggccgc ttttgcggtt gccgtcaagc gggaagtccg
     1861 cccaaggggt gtagaaaatg ggaacgccgc cgaacacgaa ggcggcgtgt ttggcaacgc
     1921 ctatgccttt ttcccgatcg gcttcgacgg aagcggcctt gacataccag ccggcatcgc
     1981 cggcggaaca ggtgttgaat tgggtttccg tcagtttgta atgcccttcg cccaacattt
     2041 cggcggtgcg gctgacgctt tgcagccgcc gtccgccgtg ttcggtttcc atacggacgt
     2101 tgtgcgcttc cccggtctgc tgctcgagat tgtaggtcag ggtttcgccc cgaatcagcg
     2161 taccgtcctg ttggagggcg aaccggtcgc ctgcggtaac ggtgtcgccc gactggtcgt
     2221 aatccgccca atcggtattg agggtcgtcc ggttgcgttc gacgacgacg ttgccttcgg
     2281 cacgcacctg cacctgcgac tgtccttcca ccttgtcggc aacaatgcgc gtatagtctt
     2341 cggggatgga tgcttcgccg ctgcctcgga cggcggcttc ggttctctcg gggctgccgc
     2401 tttcgttact gcaaaacagg caggtcgaac cgaggctcag gctggtaggc tgtatgggtt
     2461 cggacacgct ccgaacgctt cctccggcgg tcggattgtc cgtttcctcc gccgcaacgg
     2521 catcggcggc ggcgcaatgc gtgccgaaac agaagcccaa tgccagcacc agtggtttga
     2581 gtgaaaataa acgagccaaa atcgcccctc aagtcggttt gccagttaga atagcgttta
     2641 ttgtaacctg aaatgcttta gtactgttat gcaacggcaa accgaactga aaaattggct
     2701 tcagaccatc tatcccgaac gggacttcga tctgtccttc gcggcggcgg atgctgattt
     2761 ccgccgctat ttccgtgcaa cgttttcaga cggcagcagt gtcgtctgca tggatgcacc
     2821 gcccgacaag atgagtgtcg caccttattt gaaagtgcag aaactgtttg acatggtcaa
     2881 tgtaccgcag gtattgcacg cggacacgga tttggggttt gtggtattga acgacttggg
     2941 caatacgacg tttttgaccg cgatgcttca ggagcagggc gaagcggcgc acaaagccct
     3001 gcttttggag gcaatcggcg agctggtcga attgcagaag gcaagccgtg aaggggtttt
     3061 gcccgaatat gaccgtgaaa cgatgctgcg cgaaatcaac ctgttcccgg aatggtttgt
     3121 cgcaaaagaa ttggggcgcg aattaacatt caaacaacgc caactttggc agcaaaccgt
     3181 cgatacgctg ctgccgcccc tgttggcgca gcccaaagtc tatgtgcacc gcgactttat
     3241 cgtccgcaac ctgatgctga cgcgcggcag accgggcgtt ttagacttcc aagacgcgct
     3301 ttacggcccg atttcctacg atttggtgtc gctgttgcgc gatgccttta tcgaatggga
     3361 agaagaattt gtcttggact tggttatccg ctactgggaa aaggcgcggg ctgccggctt
     3421 gcccgtcccc gaagcgtttg acgagtttta ccgctggttc gaatggatgg gcgtgcagcg
     3481 gcacttgaag gttgcaggca tcttcgcacg cctgtactac cgtgacggca aagacaaata
     3541 ccgtcccgaa atcccgcgtt tcttaaacta tctgcgccgc gtatcgcgcc gttatgccga
     3601 actcgccccg ctctacgcgc tcttggtcga actggtcggc gatgaagaac tggaaacggg
     3661 ctttacgttt taaacccaat caaaatgccg tctgaaaacc aagtttcaga cggcattttt
     3721 caaacgggct tactgcgcgg ctttttgttc ttcgcgtact ttgtccgcca aaaggtcgat
     3781 ggtgttcata ccggactccc agtcggcaaa ttcaacttta tatttaccgc cgacgataac
     3841 cgtgggcgta ccgtcgattt ggaaggtttc ggtcagctcc tgcattttgt cggcgcgcgc
     3901 ctggctttcg ggggactcgt aggcggcaag gacttttttg ccgtcaaagg cggtttgttc
     3961 gcccagccat tttttgagga cttccggatt ttgcagcttg attttttggt tgaccatcgc
     4021 atcgaaaata tggctgttcg ccacatcttt gctgtcggcg gcagccatat cgacggcggc
     4081 ggcgaggcgt gccagcgtca gcatttcttt ctgccagacg acgtgttcgg tacgcaggta
     4141 catatcgtct ttaaaagact tggcgtgttt gcttaaaaca ggttcgaggt gggcgcagtg
     4201 cggacagaaa tagccgaaaa actcaaggac ttcgactttg cctgcctgct gttggggaat
     4261 cgggttggca aggacggtat agttttgccc ttcgaccagc cctgccgggg cggcggctgc
     4321 cgaagcggca ggcgcgctgt cggcggggac gctggtttgg actttgctgt cgcacgcggc
     4381 aagggcgaac agggcggcaa cgccgagggc gaggtgtctg gatttcatac ggctctccgt
     4441 gatgttggaa acagatgttg gaaatcaatc ggaatccggc tattttattg catttccgcg
     4501 tgttgataca gtttgccgcc ggaaaaggac gttttcgttt ggggaaaccg cttcggacgg
     4561 catcaaaccc gatgccgtct gaagcggttt ctgtcgtaca atacgcgccg tcgccccaga
     4621 cggatacggc tgttggggaa ctaatgatgt tccaacgcac aggacggcac ataaagcacc
     4681 gccctatgcg ttgccctggt tcggaagggt tacacccctt tcaaatatag tggattaaat
     4741 ttaaaccagt acagcgttgc ctcgccttgc cgtactattt gtactgtctg tggcttcgtc
     4801 gccttgtcct gatttaaatt taatccacta tcaaagtctg atcctgccgc cctaaagggc
     4861 ggggtttcaa ccgaaaagga aatacgatga atatgctggg agctttggca aaagtcggca
     4921 gcctgacgat ggtgtcgcgc gttttgggat ttgtgcgcga tacggtcatt gcgcgcgcat
     4981 tcggcgcggg catggcgacg gatgcgtttt ttgtcgcgtt caaactgccc aacctgcttc
     5041 gccgcgtgtt tgcggagggg gcgtttgccc aagcgtttgt gccgattttg gcggaatata
     5101 aggaaacgcg ttctaaagag gcgacggagg cttttatccg ccatgtggcg gggatgctgt
     5161 cgtttgtgct ggtcatcgtt accgcgctgg gcatacttgc cgcgccttgg gtgatttatg
     5221 tttccgcacc cggttttgcc aaagatgccg acaaatttca gctctccatc gatttgctgc
     5281 ggattacgtt cccctatatc ttattgattt cactttcctc ttttgtcggc tcggtactca
     5341 attcctatca taaattcagc attccggcgt ttacgcccac gttcctgaac gtgtcgttta
     5401 tcgtattcgc gctgtttttc gtgccgtatt tcgatcctcc cgttaccgcg ctggcttggg
     5461 cggtttttgt cggcggtatt ttgcagctcg gctttcagct cccttggttg gcgaaactgg
     5521 gttttttgaa attgcccaaa ctgaatttca aagatgcggc ggtcaaccgc gtgatgaaac
     5581 agatggcgcc tgcgattttg ggcgtgagcg tggcgcaggt ttctttggtg atcaacacga
     5641 ttttcgcgtc ttatctgcaa tcgggcagcg tttcatggat gtattacgcc gaccgcatga
     5701 tggagctgcc cagcggcgtg ctgggggcgg cactcggtac gattttgctg ccgactttgt
     5761 ccaaacactc ggcaaaccaa gatacggaac agttttccgc cctgctcgac tggggtttgc
     5821 gcctgtgtat gctgctgacg ctgccggcgg cggtcggact ggcggtgttg tcgttcccgc
     5881 tggtggcgac actgtttatg taccgcgaat ttacgctgtt tgacgcgcag atgacgcagc
     5941 acgcgctgat tgcctattct ttcggcttaa tcggtttgat tatgattaaa gtgttggcac
     6001 ccggcttcta tgcgcggcaa aacatcaaaa cgcccgtcaa aatcgccatc ttcacgctca
     6061 tctgcacgca gttgatgaac cttgccttta tcggcccact gaaacacgtc ggactttcgc
     6121 ttgccatcgg tctgggcgcg tgcatcaatg ccggattgtt gttcttcctg ttacgcagac
     6181 acggtattta ccaacctggc aagggttggg cagcgttctt agcaaaaatg ctgctctcgc
     6241 tcgccgtgat gggaggcggc ctgtatgccg cccaaatctg gctgccgttc gactgggcac
     6301 acgccggtgg aatgcaaaag gccgcccggc tcttcatcct gattgccgtc ggcggcggac
     6361 tgtatttcgc atctctggcg gctttgggct tccgtccgcg ccatttcaaa cgggtggaaa
     6421 gctgaccgat gccgatatcc tttttcggaa aacgcttttt ccgcgcctat attcgggcgc
     6481 gcatcccccg cgtccgcacc agccggacgg gctgccgcat catcaatgcc gtctgaaaac
     6541 aagaaaaccg ataccttatg attttaacac cgccggacac gccctttttc ctccgcaacg
     6601 gcaatgccga cacgattgcc gccaaattcc tgcaacgccc cgcacccaca taccgccgcg
     6661 agctgcttcc cgacagcacg ggtaaaacca aagtcgccta cgacttttca gacggcattt
     6721 cgcccgatgc gccgctggtc gtgctgtttc acgggttgga gggcggcagt ggcagccatt
     6781 acgcggttga actgatgctt gcggtacgcg atcggggttg gaacggcgta gtcgtccatt
     6841 tccgcagctg cggcggcgta gcgaacaccg ccccagtgtt ctaccacttg ggcgataccg
     6901 ccgaaatcgc ctttactttg gacacgctcg ccgcgcgtta ccgtgaaata tacgccgtcg
     6961 gcgtatcgct gggcggcaac gcgctggcaa aatatttggg cgaacagggc gaaaacgcgc
     7021 tgccgcaagc ctcggccgcc atctccgccc ccgtcgatgc agaggcggca ggcagccgct
     7081 tcgacagcgg catcacacgg ctgctctaca cgcgctactt cctccgcacc ctgataccca
     7141 aagcacggtc gctccacggt tttcagacgg catttgccgc agggtgcaaa acactgggcg
     7201 agtttgacga ccgcttcacc gcaccgctgc acggctttgc cgaccggcac gactactacc
     7261 gccgaacttc ctgcaaaccg ctgctcaaac acgttgccaa accgctgctc ctgctcaatg
     7321 ccgccaacga ccccttcctg ccgcccgaag cgctgccgac accggacgaa gtgtccgaag
     7381 ccgttaccct gttccagccg gcacacggcg gacacgccgg ctttgtcggc agcaccggcg
     7441 gcaggctgca cctgcaatgg ctgccgcaga ccgtcctgtc ctatttcgac agcttccgca
     7501 caaacaggcg ttaacggttt gatgctaata ttcccccttt ccccggacga atacggaaca
     7561 caatatgacc gacatcctca ataaaatcct taccgccaag gcacaggaag ttgccgccca
     7621 aaaagccgcc gttcccctcg aacacatccg cgcacttgcc gcagaagccg cgcccgtccg
     7681 aagcttcatc ggttcgatac gcggcaaaca ccgcctaaac ctgcccgccg tcattgccga
     7741 aatcaaaaag gcaagcccga gcaaaggtct gatccgtccg gacttccgac cggccgagat
     7801 tgcacgcgcc tatgaaaacg ccggagcggc gtgtttgtcc gtactgaccg acgaacccta
     7861 tttccaaggt tcgcccgaat acctcaaaca ggcgcgcgaa gccgtatcgc tgcccgtgct
     7921 gcgcaaagac ttcatcatcg acgaatacca ggtttatcag gcgcgcgcat ggggggcgga
     7981 tgccgtcctg ctgattgccg cagcactgga acaggaacaa ttggaacgct ttgaagcggt
     8041 ggcgcacgaa ttgggcatga ccgtcctgct cgagctgcac gatgaaaccg aattggaaaa
     8101 atgccgcaac ctgaccacgc cgttgtgggg cgtaaacaac cgcaacctga ggacttttga
     8161 agtctccctc gaccaaaccc tgtcgctgct gcccgcgctg gaaggcaaaa ccgtcgtaac
     8221 cgaaagcggc attacaggca aggcggatgt ggaatttatg caatcgcgcg gtgtgcacac
     8281 cttcctgatc ggcgaaacgt tcatgcgtgc cggcgatatt gaggcggaag tgggcaaact
     8341 cttctaaatc ccgatttcag acggcatatt gccgccgacc gatcaacatc cttaccgagc
     8401 acggaaccat catgcaccga ccgaccgcca aacagatttt gcacgaagta ttcggttatc
     8461 ccgaatttcg cggcaggcag gaggctgtca tcaatacttt ggcaggcggc gagagtttga
     8521 tggtgctgat gccgacgggc ggcggcaagt ctttgtgtta ccagattccc gcgctgatgc
     8581 gcgaaggcgt ggcggttgtc gtatcgccgc tgattgcgtt gatgaacgac caagtggcaa
     8641 acctgcacgc cgccggcatc gaagcggcgg cagtcaacag cggcacatcg gcagatgagg
     8701 cgcgcgagat tgccgacaag cttgcccaag gccgtctgaa actgctttat gtcgcaccgg
     8761 aacgcttggt taccgaccgc tttttgcgtt ttctcgacca acaaaccgtc agtctgtttg
     8821 ccattgacga ggcgcattgt gtcagccagt gggggcacga tttccgcccc gaataccaac
     8881 agctcggcat ccttgccgaa cgctatccga acgtcccgcg catcgccctg accgctaccg
     8941 ccgatgccgc cacgcgcgcc gacatcaagc attatctgca cttggacaat gcgcccgaat
     9001 ttgtctccag cttcgaccgt ccgaatattt attatcaggt tatcgaaaaa aacaacggca
     9061 aaaaacaatt gctggatttc atccgcaaag aaatgacggg gcaaagcggc attgtgtatt
     9121 gcctaagccg caaaaaggtt gaagatgtgg cgcagttttt gcgtgaaaac ggattaaacg
     9181 cgattccgta tcatgctggt ttgagcatgg acgtgcgcga ggaaaaccaa cgccgcttta
     9241 cgcatgaaga caatattatc gtggtggcga ccgtggcgtt cggcatgggc atagacaaac
     9301 ccgacgtgcg ctttgtcgcc catctcgata tgccccagag tgtcgaacat ttctatcagg
     9361 aatcggggcg tgccggcagg gacgggctgc ctgccgcgag ctggctgtgt tacggtttga
     9421 acgattgggt gttgctgcgc gaacggattg ccgaaggcaa cagcgacgag gtgcaaaagc
     9481 aaatcgaaat gcaaaaactc gatgccatgc tttccgtctg cgaaaccgcc gcctgccgcc
     9541 gcgtactgct gctcaaacat ttcggcgaag catccgaacc ctgcggccat tgcgacaact
     9601 gcctgcatcc gcccgtacgg tttgacggca cggtgttggt gcaaaaactc ctaagctgcg
     9661 tgtaccgcgc cggacaacgt tttgccgccg gttacatcac caaccttttg cgcggcaaaa
     9721 gcgacgattg gatacgcggc aaccggcacg agcaactgtc cacattcggc atcggtgcgg
     9781 agttgtccga caaagaatgg cgcagcgtca tccgccagtg catcagcctc ggctacctca
     9841 ccgtcaacat tacccgatat caggcattgc aactgaccga agccgccaaa aaagtcctca
     9901 aaggcgaaac cgaagtgatg ctgcgtccgc tcaagcgcga caagcccgcc gtccgcaccc
     9961 tcaaagacaa ctggctgcgt accgaacgcg aagaacgcct gtggcaggca ttgcgcgttt
    10021 ggcgtatgaa acaggcagaa gccgaaggca tccccgccta tatgattttc ggcgacaaaa
    10081 ccctgcgcga ccttgtcgaa aaaatgccgc aaaatctcaa cgggctgcac gacatctacg
    10141 gcttgggcga agccaaaatc gaccgtttcg gacacggcat actcaaagtc tgccaaaacg
    10201 ctgccgactt tagccacgat gccgtcatcc gtccgcaaac cgaacgcgaa cagcaactgc
    10261 gtcaaaaact cgaagcctgg cggtatgaac aggcaacggc ggaaaactgc gccctgcatg
    10321 ccgtcctctc cgacgaaagc cttgccgata tgcttgccgc cacgcctgaa accgaaaccg
    10381 acctcgaagg cgtacacggc ttgggcagcg tacgcgccgc caaatacgga cgggacatcc
    10441 tcgccgtctg ccgtccgttt tcagacggca tcgatgaagc cgccaaacgc aaacgccgcc
    10501 tgatgcgcgc cctgatccaa tggtgcggcg aaacggcaaa acacgaacag tccgaaccct
    10561 accgcattct cagcaaagcc gccctgcgcg ccattgccgc caaacagccg gaaggtttgg
    10621 cggagcttgc cgccgtatat ggcgtaggcg aagaaaaagc cgcacgttac ggtgcggcgg
    10681 tgttgacggt gttgggacaa aatgccgtct gaagcccgtt accaagtttc agacggcatt
    10741 gcctctattt aaaaattcc
//
LOCUS       DAWKQJ010000056        10645 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000056 DAWKQJ010000000
VERSION     DAWKQJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.056"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WJL20_001082"
     CDS             complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WJL20_001082"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ7683244.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1299..1988)
                     /locus_tag="WJL20_001083"
     CDS             complement(1299..1988)
                     /locus_tag="WJL20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ7683245.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLVAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            2185..2964
                     /gene="rsmA"
                     /locus_tag="WJL20_001084"
     CDS             2185..2964
                     /gene="rsmA"
                     /locus_tag="WJL20_001084"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ7683246.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVDINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3137..3865
                     /locus_tag="WJL20_001085"
     CDS             3137..3865
                     /locus_tag="WJL20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683247.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3938..4297
                     /locus_tag="WJL20_001086"
     CDS             3938..4297
                     /locus_tag="WJL20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683248.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDINKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4302..4748
                     /locus_tag="WJL20_001087"
     CDS             4302..4748
                     /locus_tag="WJL20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ7683249.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            4780..6054
                     /gene="folC"
                     /locus_tag="WJL20_001088"
     CDS             4780..6054
                     /gene="folC"
                     /locus_tag="WJL20_001088"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ7683250.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6067..7065
                     /gene="ftsN"
                     /locus_tag="WJL20_001089"
     CDS             6067..7065
                     /gene="ftsN"
                     /locus_tag="WJL20_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7683251.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7058..7555
                     /locus_tag="WJL20_001090"
     CDS             7058..7555
                     /locus_tag="WJL20_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ7683252.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            7764..9308
                     /gene="purF"
                     /locus_tag="WJL20_001091"
     CDS             7764..9308
                     /gene="purF"
                     /locus_tag="WJL20_001091"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ7683253.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            9408..9899
                     /gene="greB"
                     /locus_tag="WJL20_001092"
     CDS             9408..9899
                     /gene="greB"
                     /locus_tag="WJL20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ7683254.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            9958..10584
                     /locus_tag="WJL20_001093"
     CDS             9958..10584
                     /locus_tag="WJL20_001093"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ7683255.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         2651 a         3077 c         2758 g         2159 t
ORIGIN      
        1 cagacggacg aggttacagt ttaatccctt tgagcttcgc gacggtattg atgtctttgt
       61 cgccacgacc ggataggttg accagaatca cttggtcttt gcccattttc ggcgcgtttt
      121 tcaccgccca agcaacggcg tggctggatt cgagcgcggg gatgattccc tcgaagcggc
      181 agagcaaatc aaaggcttcg agtgcttcgt cgtctttagc gacggtgtat tcgacgcgct
      241 tgatgtcgtg cagatggctg tgttccgggc cgatgccggg gtaatccaag cctgcggaaa
      301 cagagtgtgt acccaagact tgaccgtttt cgtcctgcat cagatagctg cggaaaccgt
      361 gcaatacgcc aatcggtgag cccgaagtaa tcggcgcggc gtgatcgggg gtgttcacgc
      421 ccaaaccgcc agcctccacg ccgacgaggc gcacgttttc ttcgccgata tacgggtgga
      481 acaaaccaat ggcgttcgat ccgccgccca cgcaggcaac ggcaacgtcg ggctgtctgc
      541 cgatggcttc caacatttgt tctttggctt cattgccgat cacgcattga aagtcgcgca
      601 ccatttcggg atacggcgcg ggaccggcgg cggtaccgat gatgtagaat gtgtcatcca
      661 cgcgggcgac ccattcgcgc atggcttcgt tcatcgcgtc tttcaaagta cggctgccgc
      721 tgtcaacgcc gacgacattc gcgcccaata atttcatacg gaacacgttg ggcatttggc
      781 gttggatgtc gtccgcaccc atgtacacgt cgcaagtcat gccgaagcgg gcggcaacgg
      841 tggcactcgc cacgccgtgc tgacccgcgc cggtttcggc gatgacgcgt tttttaccca
      901 tacgttttgc caacagtgcc tgaccgatgg tgttgttgac tttgtgcgcg ccggtgtggt
      961 tcaagtcttc gcgcttcaac cagatttgcg caccgcccaa atgctcggac aaccgcgcgg
     1021 cgtggtaaac ggggctgggc ctgccgacat aatgtttcaa atcgcggtgg aattcttccc
     1081 aaaacgacgg gtcgtttttc gctgccttat aggcatccgc cagctcttgc aaagcgggaa
     1141 tcagggtctc ggacacgaac agcccgccgt gttcgccgaa aaagcccttc tcgtcgggcg
     1201 cgtggtagtt cttcatcgga ttcttccttg tgcttcaggt tttcaaacgg catttccgtt
     1261 ttttcaaaca tgccgtccga acgggaaatt gtcggcaatt ataactgctt ttcacacaag
     1321 cccgccattt cgaaaaacgc ccgatacacc gccgcctttt tcgccaaact caaaggatag
     1381 gcgcgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc gtccggcaaa cggaaacggc
     1441 acggtttcgc ccgttttcgg cattttgatg ccgccaccct gaatatcgag caggatttcc
     1501 gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg gtatttttgc caacaccgcc
     1561 accaaatcga cggtttcaac tacctttaaa aacttgtcgg acgcattgcc gtgttccctt
     1621 gccgccttca acacggtcgg acaggacgca attccccgtt cgcgcaaaaa cgccttgatt
     1681 ttctcggcat caaacgcttt ttcagacaac ctttggaaat gcgccgcatc attaaaaaac
     1741 accagcccgt aaacgcgcca catatcgttt tggaaattcg gataatgaaa ctgcatcgcg
     1801 cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga ccgcagcctt cggcggcaac
     1861 acggaatcga agggatgggt ttcgatttca agcagatttt cagacacgtt caatcctcca
     1921 acaggcaaaa cggcagacag ccggtttttg caccaaaccg ccaaacacgg caagccttca
     1981 aacagcatta tcacgccctg ccgttttcgg ttacaatgcc gacatcaaac ggatactgta
     2041 aacacattta tgttccaaca cacaggacga cacttaaagc accgccctat gtgccatcct
     2101 gatttggaag gggttacgcc cctcccaaat agagtctgat tctaccgccc taaagggcgg
     2161 ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag cccgcaagcg tttcgggcag
     2221 aattttttgc aggacacgcg gattatcagc gatattgtca acgccgtgcg cccgcaggcg
     2281 gatgatgtcg tgattgaaat cggcccgggt ttggcggcga ttaccgaacc tttggcgaaa
     2341 aagctgaacc gcctgcacgt tgtcgaaatc gaccgcgaca tcgtatgccg tctgaaaacg
     2401 ctgccgtttg cggataaact ggtgattcac gaaggcgatg tattgcagtt tgatttcaac
     2461 ggcatcgcag gcaaaaagaa aatcgtcggc aacctgccct acaacatttc cacgccgctt
     2521 ttgttcaagc tggcggaggt ggcggacgat gtcgtcgata tgcactttat gctgcaaaaa
     2581 gaagtggtcg atcgtatggt tgccgcgccg aaaagcaacg actacggccg cttgggcgtg
     2641 atgttgcaat atttcttcga tatggagctg ctgattgacg tgccgcccga atcgttcgac
     2701 cccgcgccga aagtggattc cgccgtcgtc cgtatgattc cggtgaaaca ccgcatcggc
     2761 aaggcggacg atttcgagca tttcgccaaa ctggtgaaac tcgccttcca ccagcggcgc
     2821 aaaaccatac gcaacaacct gaaagagctt gcaggcgacg atgatttgca ggcagtcgac
     2881 atcaatccgc aagaccgtgc cgaacatatc gcgccggaga aatatgtggc gttgagcaat
     2941 tatctagcgg gcaaggccgt ctgaaaaggg agtaagccga tgaagaaata cctgctttta
     3001 cccctgatac tgccgcttgc agcctgcctg accaatccga acgaccgttt ttttgacggc
     3061 agacgctaca cgcttcatca tatggagctc ccgtaacgac aacctgcaac ggcaaatgcc
     3121 gtctgaaaga aaagacatga tcaaattcaa aaacgtacac aaacatttca aagacctgca
     3181 cgtcatcaac ggcgtgaact tggaaatcaa aaaaggcgaa gtggtcgtcg tctgcgggcc
     3241 ttcgggcagc ggcaaatcca cactcatccg caccgtcaac cagcttgaaa gcatcgaaag
     3301 cggcgagatt tgggtggacg gtgtcaacgt tgccgaccca aaaaccgatt tgaacaaaat
     3361 ccgcgaagaa gtcggctttg tgtttcaaag tttcaacctc tacccgcacc tgaccgtgtt
     3421 ggaaaacatt accctcgcac cgatgaaggt taaggggcaa aatgcggaac aggcggagaa
     3481 aaaggcaatg gagcttttgg aacgcgtcgg actggcacac aaaaaagacg ccttcccttc
     3541 ccaactttcc ggcggccagc agcaacgcgt ggcgattgca cgcggtttgg cgatggaacc
     3601 gcgcgtaatg ttgtttgacg aaccgacctc cgcactcgac cccgaaatgg tcggcgaagt
     3661 gttgaaagtg atgaaagact tggcagaaag cggcatgacc atgatgtgcg taacccacga
     3721 aatggggttt gcccgcgaag ttgccgaccg cgtgattttc gtcgataaag ggcaaatcct
     3781 cgaagacgaa acgccggaag cgttttttac caacccgaaa cacgaacgcg ccaagcagtt
     3841 cctgcaacag gtcatgaccc attgatgcta tgccgtctga aaaccgaacg ttcagacagc
     3901 atatcccgac accgcaagat gattgagaaa gaaaaccatg tattttgttg accgcaccgc
     3961 cgtcgtcctc aagccgaccg cgcgcttttt ggaatggctc aaaagcaccg acgaaaatat
     4021 gcccgacctg accatagagc agcttcgtgc caactgttcc gtcttcctcg ttccgcagtt
     4081 cgacgaaccg gaagccgtcg tttcctactt cgacgaacgc taccgccaga ttttcgaggc
     4141 ggagctggca ggttgggaca tcaataagga taaatggccg caagatatgg ggctgaaggc
     4201 gttttgggaa tttttcgaca tcgaaatcca cgatatggtg ttggacatgg aagaagcgga
     4261 attgaacatt acccccgtgt tcgacaacat gatgtaaggc tgtgcgcgcc tttcagacgg
     4321 cattgaggcc gtcccgcatc ctgaacatcc tgaccgtatc gctccactcg gcttcccttg
     4381 ccgtctgcct gacctggttt tacggccgga tgatgtggtt cggtttggcg gctttggcgg
     4441 caagttacgc atactcgctc aggataacga atctgaaaca ccgccatgcc ataaccgcca
     4501 ttaccataga ccgcgacgga caggcggaaa tcgtatccgg caaagaaaaa acggcaaagg
     4561 cggcggcact gtcgggcagc agtatggtta cgccttatgc cttattctta caatgggaca
     4621 ccggcggcaa aaccgtccgc cactgcatca cccacgatat ggcggacccg gaatcctacc
     4681 ggcggctgaa agtctgggtg ctttggcggc agccgaaaaa aaccgccgaa accgatacac
     4741 caagctgatt tcagacggca tttgaacacg gaacactcaa tgaaaacact gcaagactgg
     4801 ctctcccatt tggaaaccgc ccacagcggt ggcttgatcg atatgggctt ggaacgcgta
     4861 ggcgaagtga aaaaacggat gaagctcacg ccgcaatgcc ccgtcgtcgt tgtcgcggga
     4921 acaaacggca aaggttcggt ctgcgcctac ctgacacaga tttacaaaca ggcaggcttt
     4981 aaagtcggta cgctgaccag cccgcattta ttgcgttaca acgaacgcat cgccgtcaac
     5041 gccgaacccg tttcggacga tacgattatt gcctctttcg agcgcatcga agcggcgcgc
     5101 ggcgaaatat cgctgactta ttttgaattc aatgccttgg cggcggtcga catcttcatg
     5161 cgcgaacagg ttgacgtgat gatattggaa gtcggcttgg gcggacgctt ggacgcggtc
     5221 aacgcatttg acggcgattg cgcggtggtt actagtgtag atttggacca tcaggcgttt
     5281 ttgggcgata cggtcgagca ggtcggcttt gaaaaagcag gcgtgttccg cagcggcaaa
     5341 cccgccatct gcggtcaaaa ccccgcgccc gaatcgttgc tttcacacgc cgaagccata
     5401 ggcgcgaaac tgctgatggt gcagcgcgat ttcgagtttc acgcgatgga aaacatccaa
     5461 tggaactacc gcttccggcc gcaacattca gacgaccccg cgcgcaaccg caatgccctg
     5521 cccttccccg cattgcgcgg cacataccaa ctttccaacg ccgcctgcac gttgaccgta
     5581 ttggaatgct tgaacgacaa attgccggtg gacatcggtg caatcaaacg cggattgctg
     5641 ctggttgaaa atcccggacg cttccaagtc ctgcccggcc ggccgctgac cgttttggac
     5701 gtcggacaca atccccacgc tgcccgcgcc ctgcgccgca gcctgattaa tttggcgttt
     5761 gcgcaaaaac gcaccgccgt attcagtatg ctgtccgaca aagacataga cggcgtgttg
     5821 gaaatcgtta aagaccagtt tgacgagtgg tatatcgcac cgttggacgt accgcgcggc
     5881 atgacgctcg agagcctgca aaacaaactg cacgaacaca gcatcgaaaa catacagaca
     5941 ttcgccgccg tccgcgaagc ttaccgggcg gcagcctcaa aagcgggcga gaacgacaga
     6001 atcgtcgttt tcggctcatt tcataccgtt gccgatgtca tgtcggtact ttaaaaggaa
     6061 acttctatgt ccgaaaacaa acaaaacgaa gtcctgagcg gttacgaaca actcaaacgg
     6121 cgcaaccgcc gccgcctcgt aacggcaagt tgcctggttg ccgcctcctg catcctgctg
     6181 gcagccgccc tcagttccgg ccctgccgaa cagactgccg gcgaaacaag cggcgtagaa
     6241 aacaaagcgg caggtgcggc acaaacccct gccttgaaat ccgccgccga caaaccgcag
     6301 gacttggcag gcgaagacaa gccttctgcc gccgacagcg aaatcagcga gcctgaaaac
     6361 gtaggcgcgc cgctggtgct gattaacgac cgcctcgaag acagcaacat caaaggtttg
     6421 gaagcatccg agaaactgca acaggcagaa accgccaaaa ccgcaccgaa gcaggcaaaa
     6481 caacgcgctg ccgaaaaagt gccggcaact gccgacagta cggatacggt agcggttgaa
     6541 aaaccgaaac gcactgccga aacaaaaccg caaaaagcgg aacgcactgc caaagccaag
     6601 cccaaagcca aagaaaccaa aaccgccgaa aaagttgccg acaaaccgaa aactgccgcc
     6661 gaaaaaacca aaccggatac ggcaaaatcc gacagcgcgg taaaagaagc gaaaaaagcc
     6721 gacaaggctg aaagcaaaaa aacagccgaa aaagaccgtt cggacggcaa aaaacacgaa
     6781 acggcacaaa aaaccgacaa agcggacaag accaaaaccg ccgagaagga aaaatccggt
     6841 aaaaaagccg ccattcaggc aggttatgcc gaaaaagaac gcgccttaag cctccagcgc
     6901 aaaatgaagg cggcgggtat cgattcgacc atcaccgaaa ttatgaccga caacggcaaa
     6961 gtttaccgcg tcaaatcaag caactataaa aacgcaaggg atgccgaacg cgatttgaac
     7021 aaattgcgtg tacacggtat cgccggtcag gtaacgaatg aataacctcc ccgttgccga
     7081 cctcctcgtc ttcgccatca tcgccgcctg catcgtcatt tccacgatgc gcggcgtgat
     7141 tgcggaggca ggttcgatgg cggcatgggt ggtttccttc ttttttgcca aactctttgc
     7201 cgcacccttc gccgacctcg cctttgcctc gttccaaccc cgcctgtttg cattggctct
     7261 gtcgttcatt tccctattcg tcattgcctg tctgatccag aaaatactcc gctcgctgct
     7321 gaccggggca gtttcggcgg tcggtttggg ctttgccaac cgcattttgg gcggcgtatt
     7381 cggtgcattg aaaggcattt tgattattac cctgctggtc atgctcgctt caaaaaccga
     7441 cctgcccgat accgaagaat ggcggcaatc ttacacactg ccgtttttcg tatcgctttc
     7501 cgaagccgtg ttgaaccata gcggcggcac ggcggaaact ccggaagacg attgattgcc
     7561 atacggatat ttcagacggc attctctttt atgccgtctg aaatgttttt ttcctttcct
     7621 cagccatatc acagattaaa tcttatcctc cataaaccca cctatctgtc cttcactcaa
     7681 atattgtcaa caattcaggc aaaaaaccac cgtttgcttt ataattccgt tttaccctta
     7741 aacctcgaat cggagaagac gatatgtgcg gcgtattagg tttggtcagt catgaacccg
     7801 tgaaccagct tctgtacgac ggtttgcaga tgttgcagca cagggggcag gatgcggcgg
     7861 gcattgcaac ggcggaaggc ggtaccttcc atatgcacaa aggcaaaggc atggtgcgcg
     7921 aagtgttccg cacacgcaat atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc
     7981 gttatcctac tgcgggcaac gcaggcagca gcgcggaggc gcagcctttc tacgtcagct
     8041 cgccgttcgg catcgttttg gcgcacaacg gcaacctcac caacactgcc gaactgtatg
     8101 aaaacgtgtg taacaaacac ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc
     8161 tcaacgtatt cgcgcacgaa ttacgccgcg aagtctctaa aaacgccgac ccgcaccggc
     8221 tcaatgccga caatattttc aacgccgttg cccaagtcca ccgcctggtg cgcggcgcat
     8281 acggtgtgat tgccatgatt gcgggctacg gtatgctcgc cttccgcgac ccttacggca
     8341 tccgcccgct ggtattgggt tcgcaaaccg acagcgaagg cagaaaatcc tatgccgtcg
     8401 cctccgaatc cgtcgccttc aacgcgctta cctacgattt ggaacgcgac atccggccgg
     8461 gcgaagcggt atttgtcggc tttgacggca caatgattgc ccgccaatgc agcgaccgtg
     8521 ccaaactcag cccctgcctt tttgaatatg tttattttgc ccgccccgac tctgtgattg
     8581 acggcgtatc cgtttaccaa tcgcgcttgg atatgggcgt gtcgctggcg gaaaaaatca
     8641 aacgcgagct gcccgtggac ggcatcgatg tcgtgatgcc catccccgac accagccgcc
     8701 ccagcgcgat ggagcttgcc gtccatctcg acaaacccta ccgcgagggt ttgattaaaa
     8761 accgctatat cggccgcacc tttattatgc ccggacaggc gacgcgcaaa aaatccgtgc
     8821 gccaaaaact cagcccgatg gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg
     8881 actccatcgt gcgcgggacg accagccgcg aaatcgtcga aatggtacgc gcggcgggcg
     8941 cgcgcaaagt ctatatcgcc tccgccgcgc ccgaagtgcg ctatcccaat gtgtacggca
     9001 ttgatatgcc cacgcgcgaa gagttgattg ccaacgagcg cagcgcggcg gaaatcgccg
     9061 ccgaaatcgg cgcagacggc atcgtgttcc aaaatttgag cgatttggaa gccgtcgtca
     9121 aagcactcaa tccgaaaatc gaatccttcg attcgtcctg tttcaacggc atttatcaga
     9181 ccggcgacat cgatgatgcc tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg
     9241 gtctgaaaat ccacccgagc aggatggaac acagcatcag catcagcgat gcgggcgacg
     9301 aagaataagg cttcccaaac cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg
     9361 gcatttccca tatcggcatc acatttcccg tttcatccgc acacaatatg agtaccgaaa
     9421 ccaaaaacta cattacgccc gccggatggc aggcattgaa agacgagctt tatcaactgg
     9481 tcaacaaaga acgccccgaa atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc
     9541 gcagcgaaaa cggcgactat ctttacggca aacgccgtat gcgcgaaatc gaccgccgca
     9601 tccgtttcct gaccaagcgt ttggagtccg ccgtcgtcgt cgatcccgaa ttgcgcgaag
     9661 cgaccgacca agtatttttc ggcgcgaccg tcggcctgtt gcgcggcgac gggcgcgagc
     9721 aaaccgtcaa aatcgtcggc atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga
     9781 tttcgccgct ggctcgttgt ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca
     9841 cgccggaagg gcgtgaggaa attgaaatcc tttctgtcga atacatcaaa atcgactgat
     9901 cgggaatgcc gtctgaaacg gtgcaagcgc gccgtttccc aaataaagga ataccgaatg
     9961 aggaaaatcc gcaccaaaat ctgcggcatc accacaccgg aagacgcatc ggccgccgta
    10021 gcggcaggtg cagatgccgt cgggctggtc tttttccaag gcagcagccg ggccgtcgat
    10081 attgcccgcg caaaaaaaat caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt
    10141 ttcgtcaacg aaagcgcgca aaacatccgc cgcatccttg ccgaagtgcc gatacacatc
    10201 atccaattcc acggcgacga agacgacgca ttctgccgcc agttcgaccg cccctatatc
    10261 aaggccattc gtgttcagac gacatcagac atccgaaacg ccgccgaccg cttccccgac
    10321 gctcaggcac tgcttttcga tgcctaccat ccttcggaat acggcggcac cggacaccgc
    10381 ttcgactgga cgctgctggc ggaatattcg ggcaaaccgt gggtacttgc cggcgggctg
    10441 acccctgaaa acgtcggcga agccgtccgc atcaccggag cggaagcggt cgatgtatcc
    10501 ggcggcgtgg aagcgtctaa aggcaaaaaa gacccagcca aagtcgccgc ctttatcgca
    10561 accgccaacc gcctatcccg ttaaagcaac aaaaattgcc gccggaatga cttatagtgg
    10621 attaacaaaa accagtacgg cgttg
//
LOCUS       DAWKQJ010000057        10628 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000057 DAWKQJ010000000
VERSION     DAWKQJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.057"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(158..1267)
                     /gene="mltB"
                     /locus_tag="WJL20_001094"
     CDS             complement(158..1267)
                     /gene="mltB"
                     /locus_tag="WJL20_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ7683256.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1176..1427)
                     /locus_tag="WJL20_001095"
     CDS             complement(1176..1427)
                     /locus_tag="WJL20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683257.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1468..2895)
                     /locus_tag="WJL20_001096"
     CDS             complement(1468..2895)
                     /locus_tag="WJL20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7683258.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..6806
                     /gene="mfd"
                     /locus_tag="WJL20_001097"
     CDS             3081..6806
                     /gene="mfd"
                     /locus_tag="WJL20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7683259.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSDSLKAAAVSTESSLPPGKSNLHGQ
                     IQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLL
                     RDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLI
                     SRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINE
                     LDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVA
                     VMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGT
                     VDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPR
                     TLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEV
                     DTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDI
                     PNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAAD
                     ELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLD
                     APLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLP
                     EQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYR
                     LAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6955..7338
                     /locus_tag="WJL20_001098"
     CDS             6955..7338
                     /locus_tag="WJL20_001098"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ7683260.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7360..8202
                     /gene="kdsA"
                     /locus_tag="WJL20_001099"
     CDS             7360..8202
                     /gene="kdsA"
                     /locus_tag="WJL20_001099"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ7683261.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDAIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8217..8657
                     /locus_tag="WJL20_001100"
     CDS             8217..8657
                     /locus_tag="WJL20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ7683262.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            8705..9991
                     /gene="eno"
                     /locus_tag="WJL20_001101"
     CDS             8705..9991
                     /gene="eno"
                     /locus_tag="WJL20_001101"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ7683263.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYNLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            10006..10284
                     /gene="ftsB"
                     /locus_tag="WJL20_001102"
     CDS             10006..10284
                     /gene="ftsB"
                     /locus_tag="WJL20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ7683264.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10325..10615)
                     /locus_tag="WJL20_001103"
     CDS             complement(10325..10615)
                     /locus_tag="WJL20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ7683265.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2544 a         3101 c         2718 g         2265 t
ORIGIN      
        1 tgcaaaaatg ccgtccgaac cgaaacgtgg ttcagacggc attttatagt ggattaacaa
       61 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcaaggcaac
      121 gccgtactgg tttaaattta atccactata aaaaacatta caatcccggg ccgccaagcg
      181 aattggcaat gtccctgacc gccgttacat acatccgact gtgattgtac tgccataccg
      241 tataaaaatt gttcaagccc aaataatatt caaacacgcc gggtgcggtt tccagtttga
      301 acaaaaccgc cttttcatca tctgcaagct cttcgccggg gatgatgccg tacgccttta
      361 aatccgccac cgtccgcgtc agggcggttt tttcgccaat gattgcctga acatccgcac
      421 ccggcgccaa tgttgcagac accagtattt tcccgcccgt gcgccaaccg tgctgcttca
      481 tataattggc aaccgatgcc gcgacatcgc cgatgttgcc ccatatgtcc cgatgtccgt
      541 ccccgtcata atccaccgcc catttccggt agctcgaagg cataaattgc ggcatcccca
      601 ttgcgcccgc atagctgcct ttaaaggcga aaacatcgcc gccttcttct tttgccagct
      661 ttaaaagctc gaccaattct ttttggaaaa acccggcgcg gcgggggtaa tcaaagccta
      721 aggtcgccaa tgcgtccgcc acacggaaac tgcccgtatt tttgccgtaa ttcgtttcaa
      781 tcccgataac cgccacgata agttcggcag gcacgccgta tttttgcgcc acatcatcga
      841 taagcgcgcg gttttccgca taaaaccggc gcgcgccgcg aaatttcgcc ttgcccgaat
      901 ttcccgtgcg gaacacatac cacggacgcg atgtggaggg gcggtgcata atcttgacga
      961 tgtccgcctt gtaagccgct ttgtcaaaaa aatcctgcca ttccgcccgg gaaaaatccc
     1021 ctttcccgac ttcatcgtcc acaaaacggc ggacatttgc attggcggca aacccgctgt
     1081 cggataccgg tacggctgcc gcgtcaaata cggctgccgc gtcaaacgcg gggcggcttt
     1141 cttttttcat ttcatccgcg cggggggctt gggcttcatt tgcccggggc gggcgtgcct
     1201 ccatcgccgt acaggcagac aaagccgcca aacaaattgc cagcggcagt atttttctct
     1261 ttttcataaa tatgttccga acaaataggg taagtgggaa agcggcgcaa gggcgcggcg
     1321 cgaaatgcgg tatccgccgc aatcggcggc tttgcagaat gccgcgcgtt gccttttgcg
     1381 ccgcccgaaa tctgcctgcc gccgccgaaa atgccgtcta aaggcacttc ccctttcaga
     1441 cggcattgcc tgcgccgtat ttgcccgcta tccgcaatat cggcagtcca atatgtcttt
     1501 gcggatatcg ttcagcaggc aggctgccgc gtcttcgtgt tccgcctgta cgaagacaaa
     1561 gagtcgggcg atgattttgc cgataaagac tttttgcagg gcgcaggcgt cggtcagcgt
     1621 gtgttcctgc aggaagctgc ggatgtcttc gggtaaggtg taatcacggg caacggcggc
     1681 aaagcgggca tcggtttgca ggcggtcgag cagggtttgg tttttgtcca acttcatgcc
     1741 tgcttctttt tcttcggcgg tggcgcggac aaactggtcg taaatttcgg cataaagcat
     1801 atcgttcggg ccttcaaaaa tcgtgaaggg gcggatgtcg atagcgatat tgccggcggt
     1861 gtgtccgcgt tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg cggcggcgta
     1921 agtgtattcc gtggcgaggg ttttgacgat gttcgcctcc atcagctgat gggcgacggg
     1981 ggcaacaggc gaaacggaat ggcagacgta gcggtaaaga atctcggaaa cctgatggcg
     2041 gcgccggatt tcgcggcgtt cgtaatcgac gaatctgatg tcgttgcgga cgtatcgttc
     2101 caggttttca aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt gcaggcggct
     2161 gcggataaag atgttttgga acgcgcgcaa accggcagcg tcgctctggg agagtttcat
     2221 cacggcggtt gcaggcattt cggcatcgat gcggttgacg gcgtaacgga cggcgcgcaa
     2281 gccttcggat gcgagggttt cgcagcggat gtatgttttg gggacgagca gcaggtcgat
     2341 gactttggcg agtttgccgt ttttgcgttc tttggcggca acgaggagga agtcgctttg
     2401 cgagttgccc tgccagtatt ttgcggcgtt gacgtaaatg gtttgtccgt cggtatattc
     2461 gtagtaagac tgcatttctc gggcaatcgc cgcgccggag gtttcgggtt cggtaacgcc
     2521 caaaccaccg ccctcgcctt tgaaaaccat gtccaaaccc tgtgcgattt gcgcttcatc
     2581 gccgaactct tgcagtggct gcaacaccag cgcgccttcg atgccggtac gcagcgtaac
     2641 gggcacgccg taatgccccg caatccgcaa gacttcctga atttcaaact ggctgccctt
     2701 gcgcccgccg tgttttttgt cgaggaaggg caacagcaaa cccgcctgct tcaaggcaag
     2761 ccatttgtct tcgggcaggt atcgcatcag gtcgataccg tctgaaaaaa tgcggcggaa
     2821 tgcggattcg atgtgcttta aaaaagcagc cgtgtccata gttgacggct gcgcgctcgg
     2881 ttcggtgtgt atcatcggct tcctctgtcg gttcccatta atcggcggcc ggtcaaaccg
     2941 cctgccacag tttagagttg attttctaaa ctttaccaca aagtgcgccg ggcaacaatc
     3001 cgccgacctt tcagacggca tcgcgccccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcataagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgct acgccttcag acggcatggc
     3241 ggtttttccg tccgcacgac acggcggtgt tcctgccgga ctgggaaacg ctgccttacg
     3301 agcgtttttc gccgcatcag gatttggtgt cggaacggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgttcgtgc cggttgccac ggcgatgcag aagctgccgc
     3421 ccgtgccgtt tctggcaggg cgcacgtttt ggctgaaaac agggcagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tcgatttgtt ccccatgggc agcgaaatgc
     3601 cctatcgcat cgatttgttt gacgatgaaa tcgacagcat caaaaccttt gatacggaca
     3661 cgcagcgcac catctccccc gtttccgaaa tccgcctgct gccggcacac gagttcccca
     3721 ccgacagcga ggcgcaaaag attttccgca gccgcttccg agaagaagtc gacggtaatc
     3781 cgaacgatgc ggctgtatac aaagccgtca gcaacggcca tttcggtgca ggcgtagaat
     3841 attatctgcc actgtttttt gaaaacgagc tggaaacgct gtttgactat atcggcgaag
     3901 atgcgctctt tgtctctttg ggcgatgttc atgccgaggc aaaccgcttt tggagcgacg
     3961 tcaaatcgcg ttacgccatg gcgcagggcg acgaaaccta tccgcctttg cttccacagc
     4021 atttgtatct ctctgctgat gtttttgcag gccgtctgaa aaactacgga caagtgctgc
     4081 ccgatgtttc cggcaaggaa cacaccctgc ctgaccttgc cgtcaaccgc caatccgacg
     4141 agccgttgca ggcattgaag gattttcaga cgacctttga cgggcggatt ctgctgtgcg
     4201 ccgaaagctt gggacggcgc gaaaccatgc tcggcttctt gcagcaaaac ggcttgaaag
     4261 ccaaacctgt gtccgactgg cagggctttt tatcggcgca tgagccgctg atgattacag
     4321 tggcgccgtt ggcttacggg tttaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaagga gaaggcgatg cggttaccga ccaaaccgaa ttttccgcat
     4441 ccgcaacaaa ccctctccct agccctctcc cacaggagag ggaacaaagt gcagccgccg
     4501 tttcagacag tctgaaagca gccgccgttt cagacagtct gaaagcagcc gccgtttcaa
     4561 ccgaaagcag cctgcccccc ggtaaaagta atctgcacgg gcaaatccaa cagcaacctg
     4621 ccccctcccc cgtgggggag ggttggggag agggcaaagc agttgccgct caaagtgcca
     4681 tcgccgtcat caccgaatcc gatctctacc aatacgtcgc ccgttcgcgc gtccacaacc
     4741 gccgcaagaa acacgccgcc gtttcagacg gtctgttgcg cgaccttgcc gaaatcaaca
     4801 tcggcgaccc cgtcgttcac gaagaacacg gcatcgggcg gtatatgggc ttggtaacga
     4861 tggacttggg cggcgaaacc aacgaaatga tgttgctcga atacgcagga gaagcgcagc
     4921 tttatgtgcc tgtttcacaa ctgcatttaa tcagccgcta ctccggtcag gcgcatgaaa
     4981 acattgccct gcacaagctc ggcagtggcg cgtggaacaa ggcgaagcgc aaagccgccg
     5041 aaaaggcgcg cgataccgcc gccgagttgc ttaaccttta cgcccgacgc gctgcccaat
     5101 cgggacacaa gtttgaaatc aacgagttgg attatcaggc gtttgctgac ggcttcggct
     5161 acgaggaaac cgaagaccag gccgccgcca tcgccgccgt gattaaagat ttgacgcaag
     5221 cgaagccgat ggatcgcctt gtgtgcggcg atgtcggctt cggcaaaacc gaagttgccc
     5281 tgcgcgccgc gtttgtggcg gtgatgggcg gcaaacaggt cgccgtactc gccccgacca
     5341 cgcttctggt cgagcagcac gcgcaaaact ttgccgaccg tttcgccgat ttccctgtga
     5401 aagtcgctag cctttcgcgt ttcaacaaca gcaaagccac taaagccgcg ctggaaggca
     5461 tggcggacgg cacggtcgac atcgttatcg gcacgcacaa actggtgcag gacgacatca
     5521 aattcaaaaa cttaggttta gtgattatcg acgaagaaca ccgcttcggt gtgcgccaaa
     5581 aagagcagct caaacgcctg cgtgccaatg tcgacatcct caccatgacc gccacgccga
     5641 ttccgcgtac tttaagtatg gcgttggaag gactgcgcga cttctcgctg attaccaccg
     5701 cgcccagccg ccgcctcgcc gtcaaaacct ttgtcaaacc ctttagcgaa ggcagcgtgc
     5761 gcgaagccgt gttgcgcgaa ctcaaacgcg gaggacaggt atttttcctg cacaatgaag
     5821 tagatacgat tgaaaatatg cgcgagcggc tggaaaccct gctgcccgaa gcccgcatcg
     5881 gcgtggcgca cggacaactg cgcgagcgcg agctggaaca agtcatgcgc gactttttac
     5941 agcaacgatt caacgtgttg ctctgttcca ccatcatcga aaccggtatc gacattccca
     6001 acgccaacac catcatcatc aaccgcgccg acaaattcgg actggcgcaa ctgcaccagc
     6061 ttcgcgggcg cgtcggacgc agccaccacc aagcctacgc ctacctgctc acgcccgaat
     6121 acatcaccaa agacgcagaa aaacgcctcg acgccatcgc ggcagcagac gaactcggcg
     6181 caggtttcac cctcgccatg caggatttgg aaatccgcgg cgcaggcgaa atcctcggcg
     6241 aagggcagtc cggcgaaatg atacaggtcg gcttcacgct ctacaccgaa atgctcaaac
     6301 aagccgtgcg cgatctcaaa aaaggccgcc aacccgacct cgacgcgccg ctgggcatca
     6361 ccaccgaaat caaactgcac agccccgccc tactgcccga aagctactgc cccgacatcc
     6421 acgagcgtct cgtcctctac aaacgcctcg ccgtctgcga aaccgtgcag caaatcaacg
     6481 ccatacacga agaactcgtc gaccgcttcg gcctgcccga acaacccgtc aaaaccctta
     6541 tcgaaagcca ccacctgcgg cttatggcaa aagaattggg tatcgatgcc attgatgcgg
     6601 ccggcgaagc ggtaacggta accttcggta aaaacaataa tgtcgatcca accgaaatca
     6661 tcctgctgat tcagaacgac aaaaaatacc gccttgccgg cgccgataag ctgcggttta
     6721 ccgcagagat ggaaaatatc gaggtcagaa tcaacaccgt aaaaaacgtt ttaaaaacct
     6781 tgcaaaacag atgcctgccc aaataaagcc gacaccgcaa tgccgtctga aacaccgttt
     6841 tccttgtccg aaagccgcca ttatgaattt gaaggaaact ccactataat acggcattca
     6901 gatttccaga cggcatcgcg cccgtcagac cgcacacaaa ccaaaaggaa atacatgttc
     6961 cgtactatgc ttggcggaaa aatccaccgc gccaccgtaa ccgaagccga tttgaactac
     7021 gtcggcagca ttaccgtcga tcaagatctg ttagacgcgg caggcatcta ccccaacgaa
     7081 aaagtcgcca tcgtcaacaa caacaacggc gaacgttttg aaacctatac catttcaggt
     7141 aaacgcggca gcggcgtgat ttgtctgaac ggtgctgcgg caaggctggt gcagaaaggc
     7201 gatatcgtca tcatcatgtc ttacgtccaa ctctccgaac ccgaaatcgc cgcgcatgaa
     7261 cccaaagtcg tcttggtaga cggaaacaac aaaatccgcg acatcatctc ctacgagccg
     7321 ccgcacaccg tgctgtaacc ctgaaaacgg acattgatca tggatattaa aattaacggc
     7381 attaccgtcg gtaacgatgc tcctttcgta ctgttcggcg gcatcaacgt tttagaagat
     7441 ttggattcca ccctccaaac ctgtacgcat tacgtcgaag ttacccgaaa actcggtatt
     7501 ccctatatct ttaaagcctc tttcgacaag gcaaaccgtt cctccatcca ttcttatcgc
     7561 ggcgtaggct tggaagaagg cttaaagatt tttgaaaaag tcaaagcaga gttcggcatc
     7621 cccgtcatta ccgacgtaca cgaaccccat cagtgccaac ccgtcgccga agtgtgcgat
     7681 gccatccagc ttcccgcctt tcttgcgcgg cagaccgatt tggtggccgc aatggcggaa
     7741 acgggcaatg tcatcaacat caaaaaacct cagttcctca gcccctctca aatgaaaaac
     7801 attgtggaaa aattccacga agccggcaac gggaaactga ttttatgcga acgcggcagc
     7861 agcttcggct acgacaacct cgttgtcgat atgctcggtt tcggcgtgat gaaacaaacc
     7921 tgcggcaacc tgccggttat tttcgacgtt acccattccc tgcaaacccg cgatgccggt
     7981 tctgccgcat ccggcggtcg tcgcgcacag gctttggatt tggcacttgc aggcatggca
     8041 acccgccttg ccggtctgtt cctcgaatcg caccccgatc cgaaactggc aaaatgcgac
     8101 ggccccagcg cgctgccgct gcacctttta gaagattttt taatccgcat caaagcattg
     8161 gacgatttaa tcaaatcaca accgatttta acaatcgagt aacacggttt cgccttatga
     8221 tgcagacttt ccgaaaaatc agccggtatg tcgcaacctt gtggctcggt atgcagatta
     8281 tggcgggtta tatcgccgca ccggtgctgt tcaaaatgct gcccaaaatg caggcgggcg
     8341 aaattgccgg cgtattgttc gacatcctct cttggagcgg gcttgccgtt tggggcgcgg
     8401 tgttggctgc cgcctttgcc atcctgaccc gactgcaaac cgccctgctg ctttttttat
     8461 tgtccgccct tgccgccaac caatttttgg ttacccccgt tatcgaggca ctgaaatacg
     8521 ggcatgaaaa ttggctgttg tcgtttgtag gcggatcctt cggaatgtgg cacggtattt
     8581 caagcatgat tttcatggca accgccctac tttcagcagt tttaagttgg cggctttccg
     8641 gcaaagatgc cgtctgaagc cctcccattt ttttacctcc cttcacttca cttggagaac
     8701 attcatgagc gcaatcgttg atattttcgc ccgcgaaatt ttggactcac gcggcaaccc
     8761 cacagtcgag tgtgatgtat tgctcgaatc cggcgtaatg ggacgcgcag ccgtaccgag
     8821 cggcgcgtcc accggtcaaa aagaggcttt ggaacttcgc gacggcgaca aatcccgtta
     8881 ttcgggcaag ggcgtattga aggcggtcga acacgtcaac aaccaaatcg cccaagccct
     8941 cattggtatc gatgccaacg agcaatctta tatcgaccaa atcatgatcg aattggacgg
     9001 tactgaaaac aaaggcaatt tgggtgcgaa tgcgactttg gcggtttcta tggcggttgc
     9061 acgcgccgct gccgaagact caggcctgcc gctttaccgc tacttgggcg gcgcaggccc
     9121 gatgtccctg cccgtaccga tgatgaacgt catcaacggc ggcgaacacg ccaacaacag
     9181 cctgaacatc caagagttta tgattatgcc cgtcggcgca aaatctttcc gtgaagcgtt
     9241 gcgctgcggt gcggaaattt tccacgcctt gaaaaaactg tgcgacagca aaggcttccc
     9301 gaccacagtc ggcgacgaag gcggtttcgc ccccaacctg aacagccaca aagaagccct
     9361 gcaactgatg gtcgaggcga ccgaagccgc cggctacaaa gcgggcgaag acgtattatt
     9421 cgcattggac tgcgcctcca gcgagttcta caaagacggc aaatacaact tggaagccga
     9481 aggccgctcc tacaccaacg cggaatttgc cgaatatctg gaaagtctgg tcaacgagtt
     9541 ccccatcatc tccatcgaag acggcatgga tgaaaacgac tgggaaggct ggaaactgct
     9601 gaccgaaaaa ctgggcggca aagtccaact cgttggcgac gacctcttcg ttaccaaccc
     9661 gaaaatcctt gccgaaggca ttgaaaaagg cgtggcaaac gcactattgg tcaaagtcaa
     9721 ccaaatcggt actttgagtg aaaccctgaa agccgtcgat ctggcaaaac gcaaccgcta
     9781 cgccagcgtg atgagccacc gctccggcga aaccgaagac agcaccattg ccgacttggc
     9841 agtcgccacc aactgtatgc agatcaaaac cggttctttg agccgttccg accgcatggc
     9901 gaaatacaac caactgctgc gtatcgagga agaattggcg gaagccgccg actaccccgg
     9961 caaagccgca ttctaccaac tgggcaaata agaaaaggtt aaggtatgaa gtgggtaact
    10021 gtcgttttat ccttcgcact tgtctgttgc caatacagcc tctggttcgg caaaggcagc
    10081 atcggacgca acagcagtct gagagaacag attgccgttc aagaagaaaa aaaccagaca
    10141 ctcgccctac gcaatcattc ccttgccgcc gaagtctatg atttggaaaa cggtcaagaa
    10201 gccatttcgg aaatcgcccg ggtagaactg ggttatatcc aagacggtga aaccttttac
    10261 cgactcatca ggcataaccg gtaataccgt caaaaagccg tccgaaccaa tgttcggacg
    10321 gcttttattt caacaaactg tcagacagcc cctcatcctc ccccgacaaa ccgcaatcca
    10381 gcctgacatc cccctcgacg caacagcagc acggcagtat ctcgtcccgc cccaaaaaag
    10441 ccaaaggcgg ctcccgataa gtaacgcttc cctccaaaat cttcacccgg cacgatccgc
    10501 aatatccgct tcggcactga tattccacca tatgccccgt ccgttccaag ccttccaaca
    10561 gggtctcgcc ctccaagagc tcaaaaaaac ccttattcgt accaatgcgc gccatttccg
    10621 accaatca
//
LOCUS       DAWKQJ010000058        10462 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000058 DAWKQJ010000000
VERSION     DAWKQJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.058"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(5..1006)
                     /locus_tag="WJL20_001104"
     CDS             complement(5..1006)
                     /locus_tag="WJL20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7683266.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1083..4916)
                     /locus_tag="WJL20_001105"
     CDS             complement(1083..4916)
                     /locus_tag="WJL20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ7683267.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRTALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAADKAVGRFDGSFDETRQKRQQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVCALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHNGVEFVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVQAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKTRYGAA
                     VHAAPEAKDDESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFALKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5177..5273
                     /gene="ffs"
                     /locus_tag="WJL20_001106"
     ncRNA           5177..5273
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WJL20_001106"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5441..7132
                     /gene="dld"
                     /locus_tag="WJL20_001107"
     CDS             5441..7132
                     /gene="dld"
                     /locus_tag="WJL20_001107"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ7683268.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNQQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7264..7371
                     /locus_tag="WJL20_001108"
                     /pseudo
     CDS             7264..7371
                     /locus_tag="WJL20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7427..7798
                     /locus_tag="WJL20_001109"
     CDS             7427..7798
                     /locus_tag="WJL20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ7683269.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            7953..8501
                     /locus_tag="WJL20_001110"
     CDS             7953..8501
                     /locus_tag="WJL20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7683270.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            8631..9722
                     /locus_tag="WJL20_001111"
     CDS             8631..9722
                     /locus_tag="WJL20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7683271.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAERVEGGYTVNGALPWVSNIGEEHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10193..10363
                     /locus_tag="WJL20_001112"
     CDS             10193..10363
                     /locus_tag="WJL20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ7683272.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2429 a         2917 c         2820 g         2296 t
ORIGIN      
        1 atattcaagt attcatttct cgctcaaatt cagccaagat gttccgaact tcgtccgcat
       61 ccttaaccgt tcggacgcgg tcgaacagat tttgtgctgc tgcaaattct ttcttcatca
      121 tacccagcca ctgtttcagc cgcgccagcg gatatttgtt gtttgcctct tttgtcaggc
      181 acagctcgaa aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg
      241 gcccgccgtt ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc
      301 gaccgagcat cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc
      361 tgaaaacgtc gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc
      421 attcccaatg cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc
      481 cgcatgcgcc cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt
      541 aaccgagccg catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt
      601 tgacgatgtg gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt
      661 tgacggtggg cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg
      721 cttccagcgc attcgccgcc atattgtccg catcgctgcc cagaagttgg acggtgcagg
      781 gcgtgccgga aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg
      841 atcgggaatg cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc
      901 caatacgcgt cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga
      961 tgattcgggt tttttgtttg ggttcggttg tttgtccgtc aatcatgatt cgggtaggta
     1021 tttgattcaa acaatgccgt ctgaaggctt tcggcgttca gacggcattt ccgtgtccgt
     1081 tgttacagca atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc
     1141 cagttttcgc cgaggatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg
     1201 ttgtcgtcgg tgtagcggct caagccttgc aggcaggctg ggcaggaagt aagcattttg
     1261 acgggttcac cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaat
     1321 ttgacttggg tggcgatatc gggtcgtttg agggcgaaca tgccggattc gccgcagcag
     1381 cggtcgctta agacgacttt ctgccccatc aggctgctgg ccatttgggt ggcgttcatg
     1441 gttttgatgg gcgtgtggca aggatcgtgg tagaggtatt gctgaccttt tacgccgttt
     1501 agtttcacgc ctttttcgag caggtattcg tggatgtcga tgatgcggca gccggggaag
     1561 atttcttcaa agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg
     1621 gtcttaatgt cgaggtagtt gagggtgttt gccatacggt ggaaagccac gcggttgttg
     1681 gtgctcattt cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac
     1741 atatagccgg gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca
     1801 agtccgattt ggctgaacag gcgttccgaa ccgcaacccg ggaagtagaa cacggcttcg
     1861 gcatcttcgg gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc
     1921 aataaggagc gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg
     1981 ataacctgtt ctttaattgg ggctttgccg acggttgcct tgggttcggc tttttgcttt
     2041 ttcgtgccga tcggaagaag cttgccgatt ttgtaggcaa agttttgcgc tgggaagccg
     2101 gtctgtatca tggcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac
     2161 gccataccca tagctgcggc aggcgcaaat cgtttgtggc cggaatcggc aaggtagttg
     2221 cggacggcta cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg
     2281 tggcacacgg tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg
     2341 ccgcggcggg tttgttcttc gtataagaag gcttcggtca gcaagcccac gccgaggatt
     2401 ttgttgcgcg gactgtacag caggttggca cgcggaacgt gagtagagca gacgggtttg
     2461 catttgccgc agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag atctgatttt
     2521 tccataatca gcgattccgc acccaaaagc tcgaacgacg gcgtgtaggc gttgcgtaag
     2581 tccgatcctt tcatcagttt gtggcggttg aaggtgtgtt tgggatcgac ttggtttttg
     2641 tagttccaaa acggctgcat ttcttcatcg cttaaaaatt cgagcttggt gatgccgatg
     2701 ccgtgttcgc ccgaaatcac gccgttaagc gaacgggcga ttttcataat gcgttcgacc
     2761 gaacgatagg ctgtctgaag catttcggca tcgtctgaat tgaccggaat attggtgtgg
     2821 acattgccgt cgccggcgtg catatgcagg gcgacgaaga cgcggctgcg tacggttttg
     2881 gcgtggattt tgcccaagcc ttggataatt ttggtatcgg ttttgccgct gaagatttca
     2941 gaaagcggct tcattacgtc tgctttgaca gacacgcgca ggcggaaatc gcggaaggca
     3001 ataaagcaac tttcatcgtc tttggcttcg ggcgcggcgt ggacggctgc gccgtagcgg
     3061 gtcttgtagt cggcaagcgg cgtgtcgaga ttggcgagca accagtccca acgcgctttg
     3121 acggcggaaa cgtgggccag ggcgtgtttg ccgcgttcgc ccaatagttc ggcggtcggc
     3181 aggtcggttc ccattttgtc gatggggagt ttgcccgaaa gatattgctc caatgcggca
     3241 cagagtttga gtttgttttg gatggaaagc tcgatgttga tgcgttcgat gccgtctgaa
     3301 tactcgccga gccgctccag cggaatcacc acgtcttcgt tgattttaaa ggcgttggtg
     3361 tgtttggcga tggcggcggt gcggctgcgg tcgagccaga aggttttgcg cgcttcgggc
     3421 gacacggcga taaagccttc gccgtcgcgg gcgcgggcga gttcgcagat gtgttcggcg
     3481 gctgcctgca cggcggcttc gtcgtctgaa accacatccg ccagcaagac cattttcggc
     3541 cgtcccttgc ccgccgcttt ggtggcgtag ccgacggcgc ggacataacg ccagtccaaa
     3601 tgttccaaac ccgccagccg cacgctttca tgggcaagca aaaagtcgcg gatttcgaca
     3661 atagatggcg tggcggtggc gaccgtaccg aaaaactcca tacacacggt gcgcgtgtat
     3721 ttcggcattt tgtgcaacac gaaggcgacg ccggtgatga tgccgtccgt accttctttt
     3781 tgcacgccgg gcaggccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg
     3841 cggaatttgt gaccggggat ttccaaacgt tcggttttaa cgatattgag gccgtctgaa
     3901 tccagcgtgt gaacgtcgaa cacggcggtt tcttcgtcgt ggattttgcc gaaattatgg
     3961 cgcacgcgct cgatgcgcag ccattcgccc tgagggttga ccatctgcca gtaggcgaga
     4021 ttgtccaacg ccgtccccca cagtacggct tttttgccgc ccgcgttcat cgccacattg
     4081 ccgcccacgc aggacgcatc ggcggaagtc ggatcgacgg cgaataccaa acccgcctga
     4141 tgcgcggttt cttccacccg ccgcgtaacc acgcccgcgc cgcaccggat aatcggatgt
     4201 ttgccgtcca agcccgccag ttcgacaaat tccacgccat tatgtttatc cagcttttcc
     4261 gtattgatga ccgcgctcat cgcgtccaaa ggaatcgcgc cgccggtata acccgtgccg
     4321 ccgccgcgcg gaataatgac caaatccagc tcgattaagg cgcacaccaa aggcgcgatt
     4381 tccgcctccg tgtcgggatt gacgaccaca aacggatact ccacgcgcca gtcggtcgca
     4441 tcggtaacgt gcgttacccg cgccagcccg tcgaacataa tattgtgcgg cttggtgatt
     4501 ttgctcaaac gctccaaaat ctgctgccgc ttttggcgcg tttcgtcaaa gctgccgtca
     4561 aaacgcccga ccgctttgtc cgccgccgca accaagacat ccacttgccg attgtcgtcg
     4621 cggcgtttgc ggatttcgtt caaacggtgg cgcatttccc gcaccagcgc ggtgcggcgt
     4681 ttcgggtgct ccaacaagtc atcgaccaga tatggattgc gcacaactac ccaaatatcg
     4741 cccaacactt caaacagcat ccgcgccgaa cgtccggttt tacgctgtcc gcgcaagtct
     4801 tgcagaatct gccacgcctc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag
     4861 gaagtatagt tgtaggggat ttcccgaata cgctgagggg cggtagtcgt ggtcatggtg
     4921 tgtccgtgtt tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgagttt
     4981 gaaaaggcga taatgtagtt caatttttga tacttttcaa tgccgaaaca ggaaataccc
     5041 ttatagaaac aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta
     5101 ggtggtgttt ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat
     5161 aatgccgttt ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc
     5221 ggaagccagc agcccactcc gatgcgccag tgccgggggt ttggcccgcc gccctatttg
     5281 aaacgccgga gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg
     5341 ttcagacggc attttcttta ctttgccata acgtataact acaatgggaa aacaatttca
     5401 atgtgcatat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     5461 gtcaagactg acccaaaccg taggcgaaaa atacatcatt accgaccccg cgaaaaccga
     5521 acaataccgc caaggctacc gcttcggcga aggcagggcg ttggcggtgg tgcgccccgg
     5581 aagcattctg gaaatgtgga aaattctgca ggcgtgcgtc gaagcagacg tgattgtgat
     5641 tacgcaggcg gcgaataccg gactgaccgg cggttcgacc cccgacggca acgattacga
     5701 ccgcgacatc gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacaacaa
     5761 ccaacaagtc gtctgcctgc ccggctcgac cctgaaccag cttgaattgc tgctgaaacc
     5821 tttggggcgc gaaccgcatt cggtcatcgg ctcatcctgt atcggcgcgt ccgtcttggg
     5881 cggcgtgtgc aacaactccg gcggcgcgtt ggtacagcgc ggcccggcct acaccgaaat
     5941 ggcgttgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     6001 agacttgggc gatacgccgg aagaaatcct gaccaacctg caaggtcatc attatcaaaa
     6061 caaagacatc aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg
     6121 ccaagtggac gaaccgaccg ccgcgcgttt caacgccgac cccgcgcgcc attacgaagc
     6181 ctcgggctgc gcgggcaaac tgatggtgtt cgccgtccgt ttggacacct tcccgcaaga
     6241 aaaacaaacc gccgtgttct acatcggcac caacgacatc aacgagctga ccgacatccg
     6301 ccgcgccgcc ttgggcgaat ttgaaagcct gcccgtttcc ggcgaataca tccaccgcca
     6361 cgctttcgat attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     6421 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     6481 agtcagcttc ctgcccaaac atttttccga caaggcaatg cagttcgtca gcaaattcct
     6541 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     6601 tctgaaaatg ggcggaaaag gcgtggatga agcgcgcgcg ttcttaaaag aatattttgc
     6661 acaccacggc ggcgcgtttt ttgaatgcaa cgccgaagaa acccaagccg cgatgctgca
     6721 ccgttttgcc gtcgcttccg ccgccatccg ctaccgcgcc gtgcatgatg atgaagtgga
     6781 agatttggtc gcgctcgaca tcgccctgcg ccgcgacgac cgcgattggt ttgaaaaact
     6841 gccgccggaa attgacaata aaatcatcca taaattgtat tacgggcatt ttatgtgcca
     6901 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga
     6961 aatgctgcat ctcttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     7021 tttgtatgaa gccaagcccg cgctcaaaca gttttaccgc aaactcgacc cgaccaacag
     7081 cttcaacccg ggtatcggca aaaccagcaa aaagaaaaac tgggcggaat aagcgcgtcc
     7141 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     7201 ttgaagttcc ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct
     7261 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     7321 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     7381 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     7441 aatctacacc catcccgccc tgccgcccca agccgtcaaa caaggctggt catggccggg
     7501 gctcttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     7561 gacgggcgcg atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc
     7621 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     7681 caacagcctt tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt
     7741 tgaggcagcc aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc
     7801 gcgttttctt ttccatcgtg ccgtctgaaa aatgttcaga cggcatttct tataaccttt
     7861 cgtttgcagc acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc
     7921 ccgtacccaa tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg
     7981 aaaccgcccc cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta
     8041 tccccaacct tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag
     8101 tcggcaagct caacgccgcc aacagcctga ccgccggcga agtcgaagta atccagatta
     8161 ttgccgcccg caccaaccaa tgcggcttct gcgtggcagg gcacaccaaa ctcgcaaccc
     8221 tgaaaaaact cctttccgaa caatccgtca aagccgcgcg cgctttggcg gcaggcgaat
     8281 ttgacgatgc taaactcggc gcgctcgccg cctttaccca agccgtaatg gcaaaaaaag
     8341 gcgcggtatc cgacgaggaa ctcaaagcat tttttgatgc gggctacaac cagcagcagg
     8401 cagtcgaagt cgtgatgggc gtagccttgg caactttgtg caactacgtc aacaacctcg
     8461 cccaaaccga aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat
     8521 atcgggcggt ttttcaaccc ccctcaaaca aaaacaagcc gccaaacggc agggaagcag
     8581 ccgcgcatca ggcgtgcgcc cgatttcccc gcaaacctta aggaacaaag atgaacgccc
     8641 aaaccctgat tgccaacgtt gccgaactcg tcaaaaccaa gctcaaaccc atagtggacg
     8701 acatcgaccg caaaggatac taccccgaag catttatgcg cgaactcggc gcaatcggcg
     8761 gattcggcgc agtcggcaca gaagccgaag gcggcaacgg cttgggtttg gcaacgcaaa
     8821 tcgccgtatt gcgcgaaatc ggcaaagaat gcggcgcaac ctccttcagc gcgtggtgtc
     8881 aggcggcttg cgcgtggtat ctgcatcaaa cgcccaacca agccgtcaaa gacaaatacc
     8941 tcgccgacat cctgcaaggc aaagtattgg caggcaccgg aatgtccaat accgtcaaac
     9001 accttgccgg cattgaaaaa cacaaccttc aagccgagcg cgtggagggc ggttatacag
     9061 tcaacggcgc gctgccgtgg gtatccaaca tcggcgaaga gcatatttgg gcaaataccg
     9121 cccaaatcgg cggcggctac gtcatgttta tcaccggcgg acaatgggaa ggcgtaagcc
     9181 tgcaaaactg ccccgaattt tgcgccctcg aaggcacgcg caccttcagc ctgaacttca
     9241 aagacgtatt tatccccgac gaagacatca tcgccgcgcc cgaacagttt gccgactaca
     9301 tccaaagcat caaagcaggc tttatcctcc tgcaaatcgg catcggcgcg ggcgtgatag
     9361 acggcagcct cggcatcatc cgcctcgcca acatcgtcaa cgccgaagtc aacagctatc
     9421 tcgacgacgg ctacgacagc ctcaaagcaa ggctggacgg cgcgtgggca gaaaccgaac
     9481 ggctcgccgg attggcttgg agcggcacgc ccgacaacct tgctaccctc aagttgcgcg
     9541 aagccgccgc cgtactggca cttgccgccg cccaatccgc cgccctgcat tccggcgcaa
     9601 aaggctacct gatgcgcagt cccgcccaaa ggcgcgtccg cgaagcaatg tttgtcgcca
     9661 tcgtaacccc cgcgatcaaa cacctccgaa aagaaatagc ggcaatcgaa gccgcaaaat
     9721 aaacaggcgg aaaaaaccga aacccccacc gcgtcattcc cgcgaaagcg ggaatccagc
     9781 ccccaacgca acaggaatcc atcggaaaaa acagaaaccc ccaaaccgtc attcccgcaa
     9841 aagcgggaat ccagccccct aacgcaacag gaatccatcg aaaaaaccga aacctccaaa
     9901 ccgtcattcc cacggaagtg ggaatccagt aaccgaaaaa ccacaggaat ctatcgaaaa
     9961 aaaccccacc ccccaaaaaa accgggcgga tacctcacca tcctcccaaa ccctgattta
    10021 tagcggatga acaaaaatca gggcaaggcg gcgaaaccgc agacagtaca aatagtacga
    10081 aaccgattca cttggtgctt gagcacctta gagaatcgtt ctctttgagc taaggcgagg
    10141 caacgccgta ccggtttttg ttcatccgct atacaaaaac aaggaaacac aaatggcgca
    10201 atatatgtgc ggcccctgcg gctggattta cgatgaagaa cacggcgacc ctgaacacgg
    10261 catccccccc gggacaaagt ttgaagacat tcccgacgac tggaaatgcc ccgaatgcgg
    10321 cgtgggcaaa gaagatttct acctgttgga tttcgtgata tagcggcagc cgaacccctt
    10381 cggaaaattc ctcggcgttc cgcctgcggt agcagggaac accgttttat agtggattaa
    10441 caaaaatcag gacaaggcga cg
//
LOCUS       DAWKQJ010000059        10419 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000059 DAWKQJ010000000
VERSION     DAWKQJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.059"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(24..173)
                     /locus_tag="WJL20_001113"
     CDS             complement(24..173)
                     /locus_tag="WJL20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683273.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTRRTIYKAL
                     PYVPS"
     gene            complement(191..388)
                     /locus_tag="WJL20_001114"
     CDS             complement(191..388)
                     /locus_tag="WJL20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683274.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(399..1415)
                     /locus_tag="WJL20_001115"
     CDS             complement(399..1415)
                     /locus_tag="WJL20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7683275.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(1514..2728)
                     /gene="gltS"
                     /locus_tag="WJL20_001116"
     CDS             complement(1514..2728)
                     /gene="gltS"
                     /locus_tag="WJL20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ7683276.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(2946..4352)
                     /locus_tag="WJL20_001117"
     CDS             complement(2946..4352)
                     /locus_tag="WJL20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ7683277.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            complement(4721..5980)
                     /locus_tag="WJL20_001118"
     CDS             complement(4721..5980)
                     /locus_tag="WJL20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ7683278.1"
                     /translation="MKQPVLQNNLQNLLESAENILLLQGPVGDFFLRLANWLTENGKT
                     VHKFNFNAGDDYFYPSTQAHTVVFNDSYDAFPEFLQEYIAQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKNGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVRGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVTYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGMTDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPAT"
     gene            complement(6117..8165)
                     /locus_tag="WJL20_001119"
     CDS             complement(6117..8165)
                     /locus_tag="WJL20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ7683279.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     IQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERHPDAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGYFAKKLGKIKQLYRSFK
                     "
     gene            complement(8327..8869)
                     /gene="rfbC"
                     /locus_tag="WJL20_001120"
     CDS             complement(8327..8869)
                     /gene="rfbC"
                     /locus_tag="WJL20_001120"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7683280.1"
                     /translation="MRNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERT
                     FVQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVL
                     SADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLG
                     EPSLSAKDLAGKMLAEAATF"
     gene            complement(8921..9796)
                     /gene="rfbA"
                     /locus_tag="WJL20_001121"
     CDS             complement(8921..9796)
                     /gene="rfbA"
                     /locus_tag="WJL20_001121"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7683281.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            complement(10009..>10419)
                     /locus_tag="WJL20_001122"
     CDS             complement(10009..>10419)
                     /locus_tag="WJL20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7683282.1"
                     /translation="GDGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIE
                     VVRTICALLEELAPEKPAGVARYEDLITFVPDRPGHDVRYAIDAAKIGRELGWQPQET
                     FESGIRKTVEWYLNNQTWWSRVLDGSYNRERLGS"
BASE COUNT         2431 a         2457 c         2845 g         2686 t
ORIGIN      
        1 ggaggggcgt aaccctttcc gaatcaggac ggcacatagg gcaacgcttt atatatcgtc
       61 ctgcgtgttg aaacatcagc ataggcaaca caggaaaagc cggctgtttt gcgcctgcgt
      121 accgtattgg tcaaaatgcc gtctgaaacc gataacaggg tttcagacgg catttccgtc
      181 aattttctct ttatctgccg aacaggttgg cgatattttc catctgttct tcggtaaaac
      241 cgttttcgcc caatctgacg gcaactgccc attcgcccga caaaccgtgt tccgcaccga
      301 gctgccgcca tttttcgact ttggcttcgt ctgtggtttt ttcgggcagc ggtttgacgg
      361 gaatcctgct ttttgggacg gtattcatcg gtttgcccct aatcgatgga atagccgcga
      421 agatactgta tccgttcgcg cgtcatccgc gtgtcgcatt gcagcttgag gtattcggcg
      481 tattcctgcc ggtctgcctg cgcggcggct tgtcggcagt tgctgatttt ttcctgcgcc
      541 cacttgcgtt gttcgccgac caactctttt tgcacgtcgg tatcgagtcc tccccaaagt
      601 ttggtaattt cggattccgc acgctggttt tgtacgcgcg cctcttccac ttcgccccgt
      661 gatacggtaa cggtatcggc acgctcgccg tcgtcaggat gcaggatttc cggttcgggc
      721 gcgccttctg cggcttgggg tacgtctgca tcccctccag cggcattatg ttccaaaatg
      781 tcttcgggcg tgggtttgga cggttcttct tcacgggctt ttccgctcaa aatcctgacc
      841 gcgtcttctt tttttaccgc cttgccgtct atcatcacga tgctcttcac gccgtaaggc
      901 agcaacgcga cagacagcgt ttgcgccgcc ataccgaccg tgttgtcgac aaatgccgtc
      961 tgaccgtctt tgacgggcag gaagcggacg gctgccgtca atacgccgtc tttaaactcg
     1021 acattgccgc ccgtcttctg ccgcacaata tccgacaaag cggtttcccc gtacagcagg
     1081 gggctgtttg ccttggcatc ggcaagcgtt tcagacggca cggtaatgtt caaatcggcg
     1141 atacagaacg tgcgcccgcc ttcctgcgtt tccgaagcgt gttccaaaga aaacgccaaa
     1201 ccgtaggcgg cggcgataat tttgtcggca tcgacaaact gcctgccgtc ttcgcgcgcg
     1261 aaagaacgcg cttcctgcgt gagcgtttcc tgaatattgc cgcgtatgcc ttgcaacacg
     1321 gcggggttgg cgcattccaa tgccttgggc ggttcttccc tgccgcaagc ggcaagcagc
     1381 agggcaaacg gcagcgcaat gagtttccga tacatgatgt tccttccaaa cggtgcagac
     1441 cgatagcggc tatcatagca aaagcacctg caggcggtaa accttgcagg tgctttgtcg
     1501 gaaaggcgga aaatcagcct ttaaagaaat tcacaaaacc ggtgagaatc gcggcattaa
     1561 tcaaatcgac gaagaacgcg ccgaccatag gcacaatcaa aaacgcctta tgcgacgcgc
     1621 cgaaagtatg cgtgacggac tgcatatttg ccaccgccgt cggcgttgca cccaagccga
     1681 aaccgcaatg gccggcagcc aatactgccg catcatagtc gcgccccata aagacatagg
     1741 taacaaaagt cgcgtacaaa accatcacca cggtttgtac ggcaagaatc acggttacag
     1801 gccccgccaa accggtcagc tcccacagtt tcaaattcag caacgccatt gccaagaaaa
     1861 gcgaaagcga agcattgccg aacacatcga tggcgcggtc gaacatattg accttgaatg
     1921 cggcagtgag gatgttgcgg atgaccacgc cgccaaacag acaccacacg aatttgggca
     1981 ggtcgaacag atattctttg tcgaagccgt ccataatctc ggcaaacgcc aaacacgcgg
     2041 caaacatggc aagcgtttca acggcagatt ccgccgtaat caggcgggtg cgttttgcct
     2101 gctcgaacac gtcgtccgcg ttgtcgtcct gatcctgttt tttgttttca accggtttgc
     2161 ggcccatttt gttgatcagg cggcgcgcaa ccggcccgcc gatcaggccg ccgaacacca
     2221 gcccgaaagt agccgatgca atacccaaac cggttgcgcc gaccaagccg tattgcgttt
     2281 caaaattagg tccccacgca cctgacgtac cgtgtccgcc cgtcagcgac accgaaccgg
     2341 taatcagacc gatgagcggg tccaaaccca aagccgtagc cagtccgacc ccgacaaagt
     2401 tttgcaccaa gataaatccg cccacaatcg cggtaaaaac caccagcggc aaaccgcccg
     2461 ccttcaaacg ggaaaaatcc gcgctcaagc cgatggacgt gaagaaaatc agcataaacg
     2521 cattttgcag cggtttctca aatttgaagc tcacgccgta cgcctcgtgc agcgcaaaca
     2581 ggataatcgc ggcaaccaaa ccgccagcga ccggctcggg aatattgaag tctcgtaaga
     2641 atttgatttt ttgaaccaga aatttaccaa ccagcaacac gagcgtggcg gcaatcagtg
     2701 tgtaataact gttgaattcc cattccatgt tgtttcacct cctagaaata tttttccgag
     2761 catccgtttt tgttccggat gtagtgtgtt tcaacgcttg gcgttatccg ccgagtgtca
     2821 tggtattgat gcgcggacaa ggtgttttca gaaagaaatg aaaacaatgg cgtgtccgtg
     2881 aaaaataact ggcaaatatt aggggaatag ttaaagattg tcaaaaaatg cgagtgtaaa
     2941 tcttttcaga cggccttttg tccgatggca tggtgtgctt cgcgtcgcca ttgttctaat
     3001 agggatatta atgattcggc cgaagtgtgg ccgttgccga tggcggcctg cagccagtag
     3061 caggcggtcg gaacatgaca gccgacacct tgcccgtgat agtaaaaaca ggcgaggtgg
     3121 tattgggcgg aagggtggtt ttgttcggca gcaatggcgt accagtgtgc agccgcccga
     3181 ggatcttgcg ccgtaccgag gccgtaatgg tagatgcggc ctaaggctgc cgctgcgttt
     3241 ttatggtgga acccggcagc ttccatgtaa ttttttcgcg cggcattgta gtcggcggga
     3301 cggccgatgg cgcaggcttg tgcttccgcc aaacggtaga tggtttcggt ttgttgttgt
     3361 tgcagggcgg cagttttgat ttgttcgtat tgttgcggat ggtacagcgc ggcgtcgctg
     3421 atgagttttt gtgctgcggc ggcagaaccc agcgcggcgg cacggtggta gtaatcttgc
     3481 gcgatatggt tgtcggcttt gataccgaga ccgtagcggt agaggtcgcc catgattttc
     3541 agggcttcag gatggttttt ctcggcagcg gctttggcat atcgtgccgc ttggaacgga
     3601 tcgcgttcgg aaagttcgcc ggtcagggcg tattgggcca gcttagactg tgcttccgct
     3661 tgttccttat cggcggcacg acgataccat tccaaggctt caggtttgcg ttgggcggca
     3721 agaaggtcag ccagcagcgt ttgtgcggcg atgtggccgg cttgtgcggc tggttcgaga
     3781 tagtgttcgg cttgtgcgtg gttggggctg acacctgtac cgtagaagta gatttgacca
     3841 agttgccagc aggcggcagt tacgccgagt gctgccgctt tttcgtattg ttcgatggct
     3901 gtttcaacgt cgccttggcg ttgatgatgg cgtgccagat ggtaatacgc aggtgcggca
     3961 tatcgggcgg cagcaagttc cgcccagtag agcgattgtg gatcgttgcg ttgcgcgtgg
     4021 taacgcatga ggattaattg ggcttctgag tgcccctgct cacccagcca ggcatagtcg
     4081 ttgagaagct gtccggtcgg ggtgccgttg tgttcgcgtt gttgcagcag ggagaagagt
     4141 gctttgggat ggcgttgggc cgcggcggct tccaaccatg aaagtgcctg ctccggttgg
     4201 ccgttttgca gcagatggtt gcccaatacg aaagcagctt ccgcataacc ttgctctgcc
     4261 gcctgttgca acaggggcag ggcttgggcg gtattggatt gttccaaaag ggcggcggct
     4321 tgccggtaga ggatatcagg agcggcggtc atggtttgcg ggcggatata aagtggaaga
     4381 cggatgctgc ggcatatggg cggcgatacc ggttgagggc tttgtaaaaa ttttgctcgg
     4441 cacagtttta tagagtgtat tgccttgccg gcttatcttt aggatgtttg gcttgttata
     4501 ttgttttgcc tagggatata tccatatttt gtgtgttaca aagggctcag gttgagtcgg
     4561 tatgcgctca tggcagctcg gtcattatac tgtttttgta atttattgtt aatgataaat
     4621 gcagattttt gtgttttatt tacatggcag tagctaaggt attgattgtt tcaggccgtc
     4681 tgaaatgttt tcagacggcc tgaacttttc cttcactaag tcaggttgct ggtgtagaag
     4741 agtcggaggt gtttttgttg gggaaaaaga cctgactgta gaagttgccg ttaatttggg
     4801 tcacgttgag gtggtacata cggtaggcat ggaacagctc tttgtcaggc ggtgtcggat
     4861 gattccaaaa ttctgccaag gtattttggt cagtcatgcc gggaatatca taataggcac
     4921 ggccgagaac cttaactggc atattgtgaa tcagtccgga caggccgctg gtgctgttga
     4981 tggtgaccat gccgaggccg tggcgcagga aaacgggcag ggggacatca tggacgtaag
     5041 tcacgcggcc tttgagttcg gggtgttctt tgataaagcg tttaatgtcg cgccagtagt
     5101 cgataaagcc gcggtccatc ggatgatgct tgatgatgat attggtatcg gcaggcgcgt
     5161 gctcggcaaa tgaactcaaa acatggagca ggaagctgcg gacgctggga aagtcgcaat
     5221 ggatacggac ttggctgtcg ttgaatacct gtaagggaac aataaaaaac ttgccgtatt
     5281 tgcctgcttc cacacgtttg gcgatttgga tgtcttcaat atagtagttc aaacgcttga
     5341 ggatggagag cgaccacggt ttgaggtaat ggccggcatt gggtgcgcgg tggtggatgt
     5401 agtcggggta tttgcgtgga ttgcggaaca actcgatata gtaacggata gcgttttttg
     5461 ccatgggcgt aaaaccgccg cgtaccggcg tcggcgcttt gtattcttgc tgggccagct
     5521 tggggaattg ttccaaaaaa aagtcggcac ggcgcggcaa cggggaaaat gcgttgacgc
     5581 cgtttttttc taaggtgatg tagtaggggc ggaaatagcc ttcttcaaac gcccagaaac
     5641 tggcttggtt ttcgtttgca atgcgttttg caatgacgtg ataagggcgt gtgtcgccaa
     5701 agcagacaac cgcctggatg tgatgttgag cgatgtattc ttgcaaaaat tcaggaaagg
     5761 catcgtagct gtcgttaaaa acaacggtat gcgcttgagt ggacggataa aaatagtcgt
     5821 cgcctgcatt aaagttgaat ttatgtacag ttttgccgtt ttcagtcagc caattggcaa
     5881 ggcgcagaaa aaaatcgccg acaggacctt gaagcagcag gatattttct gcgctttcaa
     5941 gcaggttttg caggttgttt tggaggacgg gctgtttcat gtcgcaatgt gattttgttt
     6001 tttatgtaat agttttgggt tgaactttca agcatacgcc aagagaatta acgatgcaaa
     6061 ggcaatgatg ccgcaattga agctggttta tgacgaatcg ttaactttga tggtatttat
     6121 ttgaaagatc tatatagttg tttgatttta cctaattttt tggcaaagta cccgcgatgt
     6181 aatccgttgt tgtttttttg catatttttt tgtcgtatca ggatttgggc agcggtttcc
     6241 gcattgatgg cctgatgggt ttcggggtgg atgtagtcgg gatagtgtat gagcgtgccg
     6301 gcaatcagct gccaaagctc aagtctgcgg ctacggcgcg gaatggggag cagatcttgg
     6361 gtaagtcccc agcctgcgta aaaaggcagg ccgtagcagc tgactttttt gccgcgcaac
     6421 aaggcttcaa aaccggtcag cgaagtcatg gtatgtattt cgtctgcgta ttggagacag
     6481 gtcaggatgt cggcttgttc ggcggtttgg tcggcatatc gtgcagcatc ttcaggggaa
     6541 atatggccga tgcggttacc gctgactaca tcgggatgcg gtttgtagat gatataggca
     6601 tcgggatggc gttcgcgtac ggtacggagc aaatccagat tgtgatagat ttggggcgaa
     6661 ccgtagcgga tagacgcatc atcctcaacc tgaccgggaa cgaggatcac ggttttgtcg
     6721 gttgacgggg cggtgaagtc tgagctgccg acgttgtatt tgctgatatg gttttcggtc
     6781 agcatttttt gcaacttcaa ggccgtctga aagtcttgat cgtcgaagtt ttggttttgt
     6841 aggatgtatt caagacggga cggggtttcg gcattgaaat aaatgctcat atcgtcggta
     6901 acgagcgaca gcggcggcac taagttggag ccgagtccga ccgagcggat aaagccgtct
     6961 tccatgcgca gcagggggat gtggtgttgt tcggcaaagc ggacgatggc ctctttgccg
     7021 ttgccccaag ccaggatgcg tgcatcgtcg gacagtttga cccttgccag tttttgggtg
     7081 gaagagataa atttcagacg gcaagagggt acgttaaaga acggtttggc aaccgcgcgt
     7141 ttccacaaag acataccgac gcaatataac tcgccacgca atttgtcgtt tttacgtttg
     7201 accgtcgcca gatagtcgat gacatcaaag aggctgcctg cttcgccggt attgggattg
     7261 aggtagcggc tgtattgcag ataggctgcg gcgaagagtt gcagcaagtt gcggggggcg
     7321 cggcgttggg tttgaataag gcggttgatt tcaggatggc ggtcgtcgct tacaccccag
     7381 ccggcatacc acggcaaacc aaacgttacc aaaggcttgc cgcacaacaa ggcttcaaaa
     7441 cccatttgcg aggtcacgca atacactttg tccaccgctt gcagcagcga aatcggatta
     7501 atgtcttcag ccaataaacg cacacgcggc tgctgtgcca aatcagtaag gtaaccctgt
     7561 ttcttgccgc tcaatacatc gggatgggtt ttgacccaga tttccgcatc aggattttcg
     7621 gctaaggccg tctgaaacat ttggcggaat gtggcttcat ctgcgccgcc atattgaatc
     7681 gccatatcgc cgaaagtttg atcaattacc aaaacaatgt ttttgctgtc ttgtacgagg
     7741 ccgtctgaaa aatccagagc atggttatat ttagacaaac ggtgcgtcaa aatcaaatca
     7801 atggcctgct gcgcttggtt aagcatttca gacggcatgt ttgcggctaa aatcaactgc
     7861 tccaaacgcg atgggcgggt tgtgtcgtaa tagatgccga tatcgtcgta aaccagtgcg
     7921 aagggcggat aaccggccac gcctaagccc agtgagcgta aaaaaccgtc ttccaaggct
     7981 acaaacggca agccatgtga ttgtgcaaaa gcacgtgctt ggtgggtggt cgggcgtaag
     8041 ccccagccaa tgatggtttg ggcgttgaat acggttttgt taagtgaata ttccggtaga
     8101 aattgcgtaa tattcggaat tttacgtata ccgaaagagc gaagaggaat gtaggcagga
     8161 atcacaataa ataggaaaaa atattactga caatataata tcttattaac agattttgtg
     8221 taaattttct gactatttaa atatagtaga aatttagtta gtaggctagc tggtaaaaat
     8281 taggtcatct tgctacaaat tacccacgat gacctaaaag tgaaaattaa aatgtggccg
     8341 cttcagcaag catcttgcct gccaaatcct tggccgatag gcttggttct cccaataaag
     8401 gccagtcgat tcccactgtg gcatcattcc aaattaggga atgttccgca ttcgggttgt
     8461 aatagtcggt gcatttgtag acgaattccg cttcatcgct caacacataa aatccatggg
     8521 caaacccttc cggcacccat aattggcgtt tgttgtctgc cgataaaacc tcaccgaccc
     8581 agtggccgaa agtaggacta tcacgacgca tatccacggc aacatcaaag acttcgcctg
     8641 ataccacgcg caccagtttg ccttgtgtat tttcggtttg ataatgtaaa ccgcgtaata
     8701 cgccttgggt ggatttgctg tggttttcct gcacgaaagt gcgctcggct acattttggc
     8761 ggaaccattc atcacggaag gtttccataa aaaaaccacg ctcgtcaccg aagactttgg
     8821 gttcgagcaa tttgacatcg ggaatgcggg tatcgatgat attcctcatg ttttcctaaa
     8881 aagcgtttca gacggcctga aaactggtaa aagcaacctc ttaatctttg ccaaccaagc
     8941 gcaataaata gcggccatat tcatttttac acattggctt tgccaagttt ctgatttctt
     9001 cgctgctcaa ccagccatta cgccatgcga tttcttccaa acaggcgact tgtaggtttt
     9061 gaatgttttc aatggtttcg acaaacgagg ctgcttccaa caggctggcg tgtgtgccgg
     9121 tatcaagcca tgcgaaaccg cgaccgagca attggacatt gagcgaacca tcgtccaaat
     9181 acatttgatt caaggtggta atttccaatt cgccgcgttc agacggcttc acttgtttgg
     9241 caaactcgac tacgcgatta tcgtagaaat agaggccggt taccgcccaa tcggatttag
     9301 gttgtttcgg tttttcttcg attgacaggg ctttgaaatt ctcatcaaac tcaatcacac
     9361 cgaaacgctc gggatctttc acttgatagc cgaatacggt agcacctttg gtttccacgg
     9421 aagcggcggc ttctttcagg gattggctga aatgctggcc atagaaaata ttgtcgccca
     9481 acaccaaaca caccgaatca tcgccgataa attcttcacc aattaaaaag gcttgcgcca
     9541 aaccatcggg gctgggttgg atggcgtagg aaatttgaat accgaaatcg ctgccatcac
     9601 ccagtaagcg gcggaaagca tcgttgtctt cgggggtggt aatcaccagc acatcgcgga
     9661 ttccggccaa catcaacacc gacaaggggt aataaatcat cggtttgttg tacaccggta
     9721 gtaattgctt tgatacgccg cgggtaatcg ggtaaaggcg gataccgctg ccgcctgcga
     9781 gaataatgcc tttcatcagt ttactttcct tatgtagttt ttcaggcggc cttcatgccg
     9841 tctgaacgta tttttattat ttgaggcctt tgcaaagcca cagatttgag tacagttcga
     9901 agttatagca gcgcagaaag cgcagacata tcacaaagat aggcaagctt tcgagcagcg
     9961 cataacgaag aaatgtgcca aagatgggga gtttgcaaag gtctctattt aactgcctaa
    10021 gcgttcacga ttgtagctgc cgtctaacac gcgcgaccac caagtttgat tgtttaaata
    10081 ccattccact gttttgcgga tgccgctttc aaaagtttct tgcggctgcc aacccaactc
    10141 acggccgatt tttgcggcat caatcgcata gcgcacatcg tggccggggc ggtctgggac
    10201 aaaggtaatc aaatcttcat accgtgccac accggccggt ttttcgggag cgagttcttc
    10261 cagcagggcg caaatggtgc gcaccacttc aatattggct ttttcattgt ggccaccaat
    10321 gttgtaagtt tcgccgattt cgccttctgt gaccacttga tacagcgcac gcgcatgatc
    10381 ttcaacaaac agccaatcac gaatctgcat accgtcgcc
//
LOCUS       DAWKQJ010000060        10233 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000060 DAWKQJ010000000
VERSION     DAWKQJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.060"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..332)
                     /locus_tag="WJL20_001123"
                     /pseudo
     CDS             complement(1..332)
                     /locus_tag="WJL20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(638..1897)
                     /gene="rho"
                     /locus_tag="WJL20_001124"
     CDS             complement(638..1897)
                     /gene="rho"
                     /locus_tag="WJL20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ7683283.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2117..4501)
                     /gene="ppsA"
                     /locus_tag="WJL20_001125"
     CDS             complement(2117..4501)
                     /gene="ppsA"
                     /locus_tag="WJL20_001125"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ7683284.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISVALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4912..5733
                     /locus_tag="WJL20_001126"
     CDS             4912..5733
                     /locus_tag="WJL20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ7683285.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5973..6635)
                     /locus_tag="WJL20_001127"
     CDS             complement(5973..6635)
                     /locus_tag="WJL20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7683286.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6648..7475)
                     /locus_tag="WJL20_001128"
     CDS             complement(6648..7475)
                     /locus_tag="WJL20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ7683287.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQSLKQNKNTV"
     gene            7905..8528
                     /gene="lolA"
                     /locus_tag="WJL20_001129"
     CDS             7905..8528
                     /gene="lolA"
                     /locus_tag="WJL20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ7683288.1"
                     /translation="MMKPHNLFQFLAVCSLTVSIVSAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8801..9940
                     /locus_tag="WJL20_001130"
     CDS             8801..9940
                     /locus_tag="WJL20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7683289.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAGQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            10072..>10233
                     /locus_tag="WJL20_001131"
     CDS             10072..>10233
                     /locus_tag="WJL20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7683290.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT         2318 a         2577 c         2850 g         2488 t
ORIGIN      
        1 cgttcgtacc cctttaaata cagctcaaaa tgcgctttgg gaatgccgtc aaacttgcgt
       61 aaatgacgtt ttgcccggtt tcaaaagttc ccaattccat tgatatggtt ttgtcgttca
      121 gcaaaataac tttcatctgc ttctactttg ccgtcaaaca tttccaaatg cggactgttt
      181 tgataaataa gtaatcgtaa acgatgaaaa taataggctg cggtattttt attaacgcct
      241 actaactctg ctgctgttct tgcagttaca cctgcgacaa acagttcaat gagtttattt
      301 tgtttatact ggcttagacg actttttctc ataggggcaa ctctaactta atttgaattt
      361 ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc ggcgcaacct
      421 tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag ccggttttgc
      481 aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc gttaacgggg
      541 ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc gattttcagg
      601 cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc atcagttcaa
      661 agaaatcgtc gttgttttta gaggctttga ttttcccgtt taagaactcg gtagcttcga
      721 tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgcaac tggtcgttcg
      781 gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg gggaagaggc
      841 gtttttccgc catacggcga tcaaggtgca attccatatt gccggtgcct ttgaattctt
      901 cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgcggtggcg ataatggtca
      961 gcgaaccgcc ttcttccacg ttgcgcgccg caccgaagaa acgtttgggg cggtgcagcg
     1021 cgttggcatc gacaccgccg gtcaggattt tgcccgaggt ggggacgacg gtattgtagg
     1081 cgcgggcaag gcgggtaatc gaatccagca ggatgaccac gtcttttttg tgttccacca
     1141 tacgcttggc tttttcaagc accatttcgg caacttggac atggcgttga gccggctcgt
     1201 caaaggtgga ggaaaccact tcgccacgga cggagcggct catttcggtt acttcttcgg
     1261 gacgttcgtc aatcaagagg acgatgagtt cgacttcggg atagtttgcg gtaacggcgt
     1321 gggcaatgtt ttgcagcatc acggttttac cgcttttggg cggggcaacc aagagggcgc
     1381 gctgaccttt gccgatgggg gaaatcaggt cgatggcgcg tccggtcagg ttttcttcgg
     1441 actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc aggttttcaa
     1501 acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggtatca agcctgacca
     1561 gggcaaaata gcgttcgttg tcttttggga cgcggacgct gccttcgatg gtgtcgcccg
     1621 tatgcaggtt gaagcggcgg atttgggtgg gcgagacata gatgtcgtcg gggccggcaa
     1681 gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt tcaagcgtgc
     1741 cggagcaggt gaaaccctcg ccttttttca tcatctggcg gacgatggca aatacgaggt
     1801 cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat tctaagagtt
     1861 tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat tttgaagagg
     1921 aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt gctcggggaa
     1981 gggggaattg taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg gggcatcggc
     2041 aggaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt ttcaggctcg
     2101 ggggtatggg cattgattat ttgttcaatt cattcgccaa atatagccaa gtttcgatga
     2161 cggtatccgg gttcagggaa acgctttcaa tgccttcctc aaccagccat ttggcgaagt
     2221 ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc ttgcggcagg
     2281 cggagatggc aagatgcagc atcactttga cggcagggtt gcgttcgtca aacgattcgg
     2341 ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg ttcgagccga
     2401 tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg ctcggcagct
     2461 cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt tctttcaggg
     2521 ctttgacaac ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg atttcaacgt
     2581 tggtcagacc catttcgtca cgaacgcgtt tcaaggcttt gcattcgagg gcgaagcagt
     2641 ctttgaaact ttcggcgaca taacgcgccg caccccggaa gcccaacatc gggttttctt
     2701 cgtgcggttc gtagatgctg ccacccacca agttagcgta ctcgttggat ttgaagtcgg
     2761 acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc acgccttcgg
     2821 cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg cgggtaattt
     2881 ccgcttttaa ttcgtcgtct tgtttgtcaa attccaacaa ggctttgggg tggataccga
     2941 tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg ggcaggttgg
     3001 cgaagctgaa tgcgagttcg ggattgccga cgttcatcat gacttttaca ggtgctttag
     3061 gcatgttgtc caaagcaaca tcggtaattt gtacgtccag caggccggag tagatgaagc
     3121 cggtatcgcc ttcggcacag gatacggtaa cttcttgacc gtttttcagc aattcggttg
     3181 cattgccgca gccgacaacg gcaggaatac ccagttcgcg cgcgatgatg gcggcgtggc
     3241 aggtacgtcc gccgcgattg gtaacgatgg cggaagcgcg tttcatcacc ggttcccaat
     3301 ccggatcggt catatcggta accagtacgt cgccggcttc gacggaatcc atctcggaag
     3361 catctttaat caggcgtact ttgccctgtc cgactttctg accgatggcg cggccttcgc
     3421 acaagacggt tttttcgccg ttgatagaaa agcggcgcag gttgcggctg ccttcttctt
     3481 gggatttcac ggtttcggga cgggcttgca ggatatagag tttgccgtcc aagccgtcgc
     3541 gaccccattc gatatccatc gggcggccgt agtgtttttc gatggtcagc gcgtaatgcg
     3601 ccaactcggt gatttcttcg tcggtaatgg agaagcggtt gcggtcttct tcggggacat
     3661 cgacgttggt taccgatttg ccggcttcgg ctttgtcggt aaaaatcatt ttgatgtgtt
     3721 tcgaacccat ggttttacgc aggatggcgg gcttgcccgc tttgagcgta ggtttgaaca
     3781 cataaaattc gtccgggttg accgcgcctt gtacgacgtt ttcgcccaga ccgtaagagg
     3841 aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac atcacacctg
     3901 atgcgccact gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg acgatgtcgt
     3961 gttcgaagcc tttgtggacg cggtaggaaa tggcgcggtc gttgtacagg gaggcgaata
     4021 catggcgcat cgcttcttta acgttttcca agccgttgat gttcaagaag gtttcctgtt
     4081 gtccggcaaa tgatgcgtcc ggcaggtctt cggcggttgc ggaagagcgt acggcaacgg
     4141 aaatgtccgc accgccggca tcggcaacca ttttgttcca tgccgcttcg atttcggcat
     4201 cgagctgttc ggggaagggc gtgtccaaaa tccattggcg gatttctttg ccgacgcgtg
     4261 ccagttcggc aacgtcttcg acatccaatt ttgccagtgc gacggaaatg cgttcgctca
     4321 gaccgttgtg tgcgaggaat gcgcggtagg catcggccgt ggtggcaaag ccgccgggga
     4381 cgcgaacgcc tttttcggtc agctgactaa tcatttcacc cagcgaggcg tttttaccgc
     4441 ccacgcgttc aacatctgtc atacgcaggt tttcaaacca gattacgtag ttgtcggcca
     4501 tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct tattttaagc
     4561 gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc cgtctgaaaa
     4621 attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatactt ctttttttgg
     4681 gtattttctt tgtaaaacag gtggtttgaa taggttaatg ttttttctgt ttgatttttt
     4741 tgtttatttt ttaaaatttt ctgccaaaaa atactttata taaataattt tatttcaaaa
     4801 ttatattgtg tctgtttggg tgtaatccga ggtaggcgtg ctgcgggtgc tttccttgtg
     4861 tctgccgctg ctgttatgat gatattttta ttctgtattt aaggatggaa gatgagcagt
     4921 ccgcgccatg tgttttacat ttccgaccgt accggtctga ctgccgagaa tatcggcgag
     4981 gctttgctga accagtttgg caatctgtcg ttcaaacgcc atacgcatcc gtttgtcgat
     5041 acgccagaaa aggcgcgcgc ggtggtggag aaggtcaatc ggagccggca ggaaaacggt
     5101 cagcgtccga ttgcgtttgt cagtgtggtt gatgacgaaa tccgtcggat tatcaaaggg
     5161 gcggatgctt ttcagattaa tttctttgag acttttttgg gactgttgga gaaagaactc
     5221 aataccgaag ccacggcatc cgagcagggg catcacagta tcggtaatac gaagcgttac
     5281 gatgcgcgta tggaggcggt gaatttctca ttgaaccacg atgacggggt cagcgataag
     5341 aaccttcagg aagcggatgt aatcttgatg ggtgtatcgc gttcgggcaa aacgccgacc
     5401 tgcctttacc tcgccctgca atacggcatc cgtgcggcaa actatccgct gattcccgac
     5461 gatttggaat cggccgatct gccgcgtatg gtcaagcctt atagggataa gctgttcggg
     5521 ttgaccatcc agccggaacg tttgcaggcc atccgccaag agcgccgccc gaattcaact
     5581 tatgccaaaa tcgatacatg ccgcagcgag gtggcggacg cgcagagtat gttcagacgg
     5641 catgggattc cgtttgcgaa tacgacggat aaatcagtgg aagagcttgc cgtacacatc
     5701 cttcaggcgt gcaagctcaa acgcaggttt tgacgggttt tgattcggtt tgaaggtgga
     5761 actgccgtct gaaatcaggt ttcagacggc agttttatag tggattaaca aaaatcagga
     5821 caaggcgacg aagccgcaga cagtacaaat agtacggagc cgattcactt ggtgcttcag
     5881 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgacgtactg gtttagattt
     5941 aatccactat atgtttgtgg ggcggatatt tttcagggct gtattttgtc cagacattcg
     6001 agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt atcgtcttcg
     6061 ggagcgatgt agccccattc ggcgaggacg gttttcatgc cggcgttgcg gcctgcttgg
     6121 atgtcgcgtt cggcatcgcc gacgtagagt gtgtgttgcg ggtcggcgtg gatttgtccg
     6181 cacgcataca gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc gccgctgacg
     6241 acgacggcgg gtgggatgat gaagccgagt ttgggggcga gtttgtcggt gaagcgcatg
     6301 ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgt cgagttcggc gatgagttcg
     6361 tttacgccgc cgaagagggt ggtgtcttgg gcgtagcggc tgtcgtattc gtcaaggtat
     6421 tcggtgcgcc atcgggcata gtcggggtgg tcgggggtga tgcctgtgcc gagcttgagc
     6481 agtcctgctg cgccgtggct ggcttggttg cggatttcgt ccatgctttt gggcggcagt
     6541 ccgtggcggg cgagcagggt attgagtgcg ccgccgaggt ctagggcggt gtcggcaagc
     6601 gtgccgtcga ggtcgaagag tacggcgtgt atcatgtatg ttccttttca gacggtattt
     6661 ttgttctgtt tcaaactctg ctcgaaatct tccagcatat ccaattcgaa gcgactgaag
     6721 cccgcttttt cgcgcgcttc gatgttaaca tagccgcgga agataaacat atcgtaacgg
     6781 gcaatcaggc tgcggaacag ggcaatcggt tccaaaccgc gttcgcggca gaggtgttga
     6841 taccaccggt tgccgatggc gacatggccc acttcgtcgc ggtaaataat gtccaacacg
     6901 ccgcaggttt ccgaatcgcc gcgctgcgcc accttcgcgc gtatccccgg cgtaacgtct
     6961 agcccacgag cttccaatac gcgcggcact aaagccatac gcaacaaagg atcgtaggcg
     7021 gttttgtatg ccatatccca taaatgattg tgcgcctcga aatcgccgta atcgaagccg
     7081 aaagcgcgca gcctttcgcg catcaggcgg aagtggtaca cctcttcctt cgccactttc
     7141 acccagtcgc ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc gtccaaagcc
     7201 agattgacgg cattgaactc gatatgcgca atcgcgtgca gcatcgccgc atagccttcg
     7261 gtcgtgttca ttttgcgcgg ggtcagttgt gacggcgcga ccaaaaccgg cttgtccggc
     7321 cgccctgcgc gggggaaatc cagcggcggc gtgtttgttt cagcaccgtc catgttctga
     7381 acggcggcaa acgcctcatc cgtcagccgt cctttttcat cggggtcgcc cgaaagcagg
     7441 gcgcgttcca gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg ttcggaaaac
     7501 aaatattata gcgtttaaaa aaaacaagat gaggcatata atctccgcga ttcggcattc
     7561 cgcgcccaaa ccgtcaaata tagtggatta acaaaaaccg gtacggcgtt gcctcgtctt
     7621 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt ttcgtactat
     7681 ttgcactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataacgcggc
     7741 acacattaaa gggcagcgtg gcgcgcctgc cttttccgag cggtcaaaaa aatcagccct
     7801 cggaaaacgc ggtttgcaaa atgcaaaccg cccgtaacgc tgcccgtatg attgttttgc
     7861 cgcgccgata ctttacgcta cacccatccc gacaaggaaa aataatgatg aaaccgcaca
     7921 acctgttcca attcctcgcc gtttgctccc tgaccgtctc catcgtttcc gcacaggcgg
     7981 gcgcggtgga cgcgctcaag caattcaaca acgatgccga cggtatcagc ggcagcttca
     8041 cccaaaccgt ccaaagcaaa aagaaaaccc aaaccgcgca cggcacgttc aaaatcctgc
     8101 gtccgggcct tttcaaatgg gaatacactt cgccctacaa acagactatt gtcggcgacg
     8161 gtcaaaccgt ctggctttat gatgtcgatt tggcacaagt gaccaagtcg tcccaagacc
     8221 aggccatagg cggcagcccc gccgccatcc tgtcgaacaa aaccgccctc gaaagcagct
     8281 acacgctgaa agaggacggt tcgtccaacg gcatcgatta tgtgctggca acgcccaaac
     8341 gcaacaacgc cggctaccaa tacatccgca tcggcttcaa aggcggcaac ctcgccgcca
     8401 tgcagcttaa agacagcttc ggcaaccaaa cctccatcag tttcggcggt ttgaatacca
     8461 atccccaact ctcgcgcggc gcgttcaagt ttaccccgcc caaaggcgtg gacgtgttga
     8521 gcaactgatg ccgtccgccc cgatgccgtc tgaacgccgc cgaggcttca gacggcattt
     8581 ttacgcaggc ggaacaatgt cccgcattac cgcccgatcg ggcaccggaa cggcaaaccg
     8641 atgaaaatta acggttgcgc cccggctgtt tttgccgttt aatgcaaacc ttgctgcacc
     8701 aagggccaag aaagccgacc ggccgccccc acagcttccg atgcaggcgg ccctgccgtc
     8761 tctgcaatgt tttttatttt tgaacgaaag gtcgaaaacc atgaaaaaaa cactggtggc
     8821 ggcggcgatc ctgagcctcg ccttgactgc gtgcggcggc ggaagcgata ccgccgccca
     8881 aaccccctcc gccaagcccg aagccggaca atcgggcaaa ctcaacatct acaactggtc
     8941 ggattatgtc gatcccgaaa ccgtcgccgc ctttgaaaaa gaaaccggca tcaagacgcg
     9001 ttccgattat tacgacagca acgaaacact ggaggcaaaa gtcctgaccg gcaaatccgg
     9061 ctacgacctg actgcgccgt ccatcgccaa cgtcggccgg caaatcaaag cgggcgcgta
     9121 tcagaaaatc gacaaggcgc aaattcccca ttacggcaac atcgataaag atttgttgaa
     9181 aatgatggaa gccgtcgatc cgggcaacga atacgccgtc ccctatttct ggggcatcaa
     9241 caccttggca atcaacacgc ggcaggtgca aaaggcattg ggtacagaca agctgcccga
     9301 aaacgagtgg gatttggtgt tcaaccccga atacactgcc aaactcaaat cctgcggcat
     9361 cagctatttc gacagcgcaa tcgaacagat tcctttggcg ttgcactatt tgggcaaaga
     9421 ccccaacagt gagaatcccg aagacatcaa agccgccgtc gatatgatga aagccgtccg
     9481 gggcgacgtg aaacgcttca gctcttccgg ctatatcgac gatatggcgg cgggcaactt
     9541 gtgtgccgcc atcggttacg gcggcgattt gaacattgcc aaaacccgtg ccgaagaagc
     9601 cgcaaacggt gtggaaatca aagtattgac cccgaaaacc ggcgtgggcg tgtgggtgga
     9661 ttcctttatg attccgcgcg acgcacaaaa cgttgccaac gcccaccgct atatcgacta
     9721 cacgctccgg cccgaggtgg cggcaaaaaa cggcagcttc gttacctacg cgcccgccag
     9781 ccgtcccgcg cgcgagctga tggatgaaaa atacacctcc gacgcatcga ttttcccgaa
     9841 caaagaactg atggaaaaaa gtttcatcgt atcgcccaaa tccgcagaat ccgccaaact
     9901 gggcgtgaag ctgtggcaag ggctcaaggc cggaaaataa ccgcaacctc tgccgtctga
     9961 aacctttcgg gcggcaggaa acggcgcgtc cgttatcaaa cagggggcgt ttccccgccc
    10021 gcctgttatg attgggttaa gattaaaatg atttagtaaa atgagaaaga tatggattta
    10081 agtatcgtag ttcccattta taatgtcgag agttatttgg aagcgtgtct aaattccata
    10141 gaacctatat taagtaatga aaatgtcgaa cttatccttg tgaatgacgg gtcaaaagac
    10201 ggaagtgaag atatatgtta taaatatata gat
//
LOCUS       DAWKQJ010000061        10207 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000061 DAWKQJ010000000
VERSION     DAWKQJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.061"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(280..1497)
                     /locus_tag="WJL20_001132"
     CDS             complement(280..1497)
                     /locus_tag="WJL20_001132"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ7683291.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1699..2427)
                     /gene="rph"
                     /locus_tag="WJL20_001133"
     CDS             complement(1699..2427)
                     /gene="rph"
                     /locus_tag="WJL20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ7683292.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2509..2973)
                     /locus_tag="WJL20_001134"
     CDS             complement(2509..2973)
                     /locus_tag="WJL20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ7683293.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3190..3966
                     /locus_tag="WJL20_001135"
     CDS             3190..3966
                     /locus_tag="WJL20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ7683294.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4211..5065
                     /locus_tag="WJL20_001136"
     CDS             4211..5065
                     /locus_tag="WJL20_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ7683295.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5106..6314
                     /gene="pncB"
                     /locus_tag="WJL20_001137"
     CDS             5106..6314
                     /gene="pncB"
                     /locus_tag="WJL20_001137"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ7683296.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6406..8124
                     /gene="argS"
                     /locus_tag="WJL20_001138"
     CDS             6406..8124
                     /gene="argS"
                     /locus_tag="WJL20_001138"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ7683297.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPDFLAQNIQTALNDT
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEAPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYNTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATVPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGATRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8690..10108
                     /locus_tag="WJL20_001139"
     CDS             8690..10108
                     /locus_tag="WJL20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7683298.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKITGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLISKKTCAIFSGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCIEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2336 a         3004 c         2584 g         2283 t
ORIGIN      
        1 gatgtatagt ggattaacaa aaccagtacg gcgttgcctc gccttagctc aaagagaacg
       61 attctctaag gtgctgaagc accaagtgaa tcagttccgt actatttgta ctgtctgcgg
      121 cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa tttaatccac tatatttttt
      181 gttccaaagt caaaatatgc cgtccgaaca ttcgggcggc agacaaaacg gcactgcccg
      241 ataaaggcag tgccgttgtc cgtttcaaac cgtgaaacat cagcccaaat taaaggcttt
      301 atgcaatacc ctggttgcca gttccatgta tttttcatca atcaatacgg aaactttgat
      361 ttcggaggtg gaaatcattt ggatgttgat accctcttcg gcgagcgtgc ggaagatttt
      421 ggcggctaca ccgacgtgcg aacgcatacc caaaccgact gcggagactt tgcacacggt
      481 gtcgtcgccg tcgatagaag ccgcgccgat actgtcttga cgttccgaca ggatttccaa
      541 agtctgcttg taatcgccgc gcggtacggt aaaggaaaaa tcggttgtgc cttcgctgcc
      601 gacattttgg ataatcatat cgacttcgat gttggcatcg gcaaccgcgc ctaaaatctg
      661 ataggcgacg ccgggtttgt cgggtacgcc gcgcacgttg atgcgggctt ggtttttgtc
      721 gaatgcgata ccggttacgg cagctctttc catattgtcg tcctcttcaa aggtaattaa
      781 ggtgccgttg ccgccgtctt gcaggctgct cagtacgcgc aggcgcactt tgtattttcc
      841 ggcaaattct actgaacgga tttgcaaaac tttcgagcca aggctggcca gctcaatcat
      901 ctcttcaaac gttaccgtat ccatgcggcg cgcttcgggt acgacgcggg ggtcggtggt
      961 gtaaacgccg tccacgtcgg tatagatttg gcattcgtct gctttgaggg ctgcggcgag
     1021 cgcaacggcg gaggtgtcgg agccgccgcg tccgagcgtg gaaatgtcgc cttcgctgct
     1081 gatgccttgg aaaccggcga cgatgacgac tttgccggcg gcaaggtcgg cacgcatttt
     1141 ttcgtcatca atgctttcga tgcgggcttt ggtgtgggcg gtatcggttt tgagggcgac
     1201 ctgccagccg gtgtagcttt tggcttccac gccgatgtct ttcaatgcca tcgccaaaag
     1261 gccgatggtt acttgttcgc cggtggacaa aaccacgtcc aattcgcgcg gatcgggatg
     1321 ctcctgcatt tcgtgcgcca acgcgactag gcggttggtt tcaccgctca tggcggatac
     1381 gacgacgacg atgtcgtgtc cttcggcgcg ggctttggcg acacgtttgg ccacgttttt
     1441 aatgcgttcg ggcgagccta cggatgtgcc gccgtattta tgtacgatta acgccatgtt
     1501 tcgtgctttc ttgtgtggga ttggtcgggc agcatggttt gctggaaaag cggctattat
     1561 tactattttt tacatgaagt tcaagaacgg actgcgcttt cccgcctgcc gtttgacagc
     1621 ggtcagcgaa aaacctgttc tttcagattg ttgacaaaat gccgtctgaa cggttttcag
     1681 acggcatccg gacgacaatc aggcggcgga caacgcattt tgctggtgtt gcagcagttc
     1741 gcctatgcct ttttgcgcca gtgcaaccag tttgcccaat tcgtccaaac tgaacggcgc
     1801 gtcttccgcc gtaccttgga tttcgatgat ttttcccgat gcggtcatga cgatattcac
     1861 atcactgtcg caaccggagt cttcgggata atccaaatcc aaaagcggca cgccgttcac
     1921 tacgcctact gacacagcgg caacggcttc gcggatgggg ttttcactca aaatgccgtc
     1981 tgaaaccagt ttgccgacgg cgatttgcag cgcgacaaac gcaccggtaa tcgaagccgt
     2041 gcgcgtaccg ccatctgcct gaatcacatc gcagtcaatc aagatttgtc gttcgccgag
     2101 tttttccata tctacgaccg cgcgtagcga acgcccgata aggcgttgga tttcttgtgt
     2161 gcgcccggac tgtttgcccg ccgaagcttc gcggcgcatc cgggaagcag ttgaggcagg
     2221 cagcatcccg tattccgccg ttacccagcc ttggttttta ccgcgcagaa acggcgggac
     2281 gttttcatct atggaagcgg tacaaatcac tttggtattg ccgcattcaa taaggcacga
     2341 accgtccgta tgcggcagga aatgaggggt gattttgata tcgcgcaggc tgtccgcggc
     2401 gcgcgagatg cggatgtaat caggcatact gccctcccgt taaaaacaga taaattaaaa
     2461 agccttaaat atgaaaaatc acatttaagg ccttcaaact gaaaatttct acgcctcttc
     2521 ggctttgctg cggataatca aaagcggcag gtggctttgg cgcattaccg tttcggcaaa
     2581 actgcccatt aaaaggtgca tcagtccggt acgtccgtgc gtacccaaca ccagcaggtc
     2641 ggcaccgttt tcatcggcat aatcaaccaa atcctgcgcc atttcacgcg cacccttatt
     2701 ggcaaccagc aggtgtttga cggtattttc cacacccagt tcccgggcgg tgcgctcggc
     2761 ggcatccaaa acttcgttgc cttgcgcgac ggcggcggct tcgtagcttt cgtgttgcaa
     2821 aaattcgggg gcgagcgcca tatattcggc aggattggca acgtgtacca aagtcagacg
     2881 cgcaccgttg accccggcaa gctcggcggc atgtttcagg gcattgatgg acgtttcact
     2941 gccgtcaacg gcaacaacca gatgtttgta catatcgtat tctccttttg caccgcctcg
     3001 cggtgccctc ttgtcggatg ggcgcaggga cagtttgcgc cgtttcatta tagacccgct
     3061 gtcgggcttt atacaacagc cgaacagccc gaccgctttc cagtataata tgccgcttcc
     3121 gtgcagtcag gcattttttg ccggctttcg ttcacttttt gatttgacgc aatcttgcag
     3181 gattcgacca tgtccgacaa cgctttgacc tcttcgcgac gcttcggcgg catcgccaga
     3241 ctctacggag actctgcctt ggcgcacttt tcacaggcac acgtctgcgt agtcggcgtg
     3301 ggcggtgtcg gctcgtgggc ggtcgaggct ttggcgcgga cgggcatcgg acgtttgact
     3361 ttgattgatt tggacaacgt tgccgaatcg aatgtcaacc gccagctgca cgccctgacc
     3421 ggcgacttcg gcaaagcaaa agttaccgcc ttgcgcgaac gcgtcgccca aatcaaccca
     3481 ggatgccaag tgtttgaaat tgaagatttc gttaccgaag acaatttgcc ggaatacttc
     3541 ggaaaaggtt ttgatttcgt catcgacgcg atcgaccaag tgcgcgtcaa agcagcaatg
     3601 gcggcttatt ttgtggaacg caaacaaccg tttgtcctca gcggcggcgc gggcggacaa
     3661 aaaaatccgg cgttaatcca aaccgccgat ttgagccgcg taacccacga cccgctgctt
     3721 gccaacctgc gctacacctt gcggaaacgc tacggattca gccgcgatac gaaagcaaat
     3781 atgcgcgtgc cttgcgtgta ttcgaccgaa aatatcgtgc cgccgcagtc tagggaggct
     3841 tgttcggcag atgccgctcc gcaaggcttg tcgtgcgccg gctacggtgc aagcatgctc
     3901 gttaccgctt cgttcgggct atattgcgca caggcggcgg tggaacacat cgcagacaaa
     3961 aaataagcaa tgccgtctga aacaggattc agacggcatt tgaacaaacg cggcaatcaa
     4021 gctgtcggtt cagaaaaacc aacatcggca cgcctaaaac cgtattcaca acctgctcct
     4081 tttcaaaaca tttgcattta aaagccgtta taatgccgtc tgaacatctg cccgaccaca
     4141 ttatgcgtga atgtcggcag attgttttct tttgtaaact tatattaaaa tccacttacc
     4201 gattcacgcc atgccgccca tccctgcccc atccgcacca tccgagcaca ctgtcgcatg
     4261 ggtattcggc caacccgtta ccgatttgcc gcaggatttg tttatcccgc ccgacgcgct
     4321 gaaagtcgta ttgggcagtt ttcaaggccc tttggatttg ctgctctacc ttatccgcaa
     4381 gcagaacatc gatgttctcg atattccgat ggtgaagatt accgagcagt atctgcacta
     4441 catcgcccaa atagaagcct atcagtttga tttggcggcg gaatacctgc ttatggcggc
     4501 catgctgatt gaaatcaaat cgcgcctgct gctgccgcgt accgaaaccg tcgaagacga
     4561 agaagccgac ccgcgtgccg agttggtgcg ccgcctgctg gcttacgaac agatgaagct
     4621 ggcggcgcag ggtttggacg cgctgccccg agccggacgg gatttcgcgt gggcttacct
     4681 gccgctggaa attgccgtcg aagccaagct gcccgaagtc tatattaccg acttgacgca
     4741 agcgtggctg ggtattttgt ctcgggcaaa acacacgcgc agccacgaag taatcaaaga
     4801 aaccatctcc gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg gacacgggat
     4861 atgcaggttt cacgacctgt tcaatcccga acagggcgcg gcttacgtgg tcgtcaactt
     4921 catcgcactg ttggagcttg ccaaagaagg tttggtcgga atcgtacagg aagtcggttt
     4981 cggagaaatc cgaatcagcc tcaatcatga gggggcacat tcagacggca tttccggcac
     5041 acggggcggg cgcgatgtgt tctaatacgc cccaagccgc caccaaaaat cgggagacac
     5101 gccatatgac cggcatcata cattcgctgc ttgacaccga cctctacaaa ttcactatgc
     5161 tgcaagtggt tctgcaccag tttccgcaga cgcacagcct ttacgaattc cgctgccgca
     5221 acgcctcgac cgtctatccg cttgccgaca tcaaggaaga cttggaagcc gaactcgacg
     5281 cgctctgccg gctgcgcttc acccacgacg aactcggcta tctgcgctcc ctgcgtttca
     5341 tcaaaagcga ctttgtcgat tatctcgaac tcttccagct ccaacgccgc tttgtcgaaa
     5401 tcggcacaga cgataaagac cgtctgaaca tccgtatcga aggtccgatg atacaggcga
     5461 tgttttttga aatcttcatc ctcgccattg tcaacgaact ttacttccgc cggctggaaa
     5521 cccctgccgt tatcgaagaa ggtgaacgcc ggcttcaagc caaagccgcg cgcctcaaag
     5581 aaatcgccgc cgcacaaaac cccgacgatc cgcccttcct gatttccgac ttcggcacgc
     5641 gccgccgcta caagctcgcg tggcaggaac acgtcatccg caccctgctc gaagccgccc
     5701 caagcatcgt gcgcggcacc agcaatgtct ttctcgccaa aaaactcggc atcaccccca
     5761 tcggcaccat ggcgcacgaa ttcctccaag ccttccaggc cctcgacgta cgcctgcgga
     5821 atttccaaaa ggccgcgctc gaaagctggg tgcacgaata ccggggcgat ttgggcgttg
     5881 ccctgaccga cgtggtcggt atggatgcct tcctgcgcga tttcgacctc tatttcgcca
     5941 aacttttcga cgggctgcgc cacgacagcg gcgaccctta cgtttggggc gacaaagcct
     6001 acgcccacta tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc ttctccgacg
     6061 ggctggacat cgaacgctct tgggcattac accaatattt caaagaccgc ttcaaaaccg
     6121 gcttcggcat cggcaccaac ctcaccaacg atatggggca tacgcctttg aatatcgtgt
     6181 tgaaactggt cgaatgcaac gggcagtccg tcgccaagct gtccgactct ccgggcaaaa
     6241 ccatgaccaa caacagcacc ttcctcgcct acctgcgcca agtgttcgac gtacccgaac
     6301 ccgaaacgcc gtaaaccggc agaaaaagcg cacaattcct gtttctgccg cataaaatct
     6361 tttaaaatac cgcctgattt gaatttaacc gaaagaccga acttcatgaa cctacatcaa
     6421 accgtcgaac gcgaagccgc cgccgccttt gccgccgcag gcatcgccga cagtcccgtt
     6481 gttttgcagc cgaccaaaaa tgccgaacac ggcgatttcc aaatcaacgg cgtgatgggt
     6541 gcggcgaaaa aagccaaaca aaatccgcgc gaattggcgc aaaaagtggc tgaagcattg
     6601 gcggacaatg ccgtgattga aagcgcggaa gtagccggcc cgggctttat caacctgcgt
     6661 ctgcgccctg atttcctcgc acaaaacatt cagacagcct tgaacgacac gcgtttcggt
     6721 gtggcaaaaa ccgacaaacc gcaaactgtc gtcatcgact attcctcgcc caatctggcc
     6781 aaagaaatgc acgtcggcca tctgcgttcc agcatcatcg gcgacagcat ttcgcgcgta
     6841 ttggaattta tgggcaacac cgtcatccgc caaaaccacg tcggcgactg gggtacgcag
     6901 ttcggcatgt tggtcgctta tttggtcgag cagcaaaaag acaatgccgc gttcgagctg
     6961 gcggatttgg agcagtttta ccgcgccgcc aaagtgcgct ttgacgaagc ccctgccttc
     7021 gccgacaccg cgcgcgaata cgttgtgaag ctgcaaggcg gtgatgaaac cgtattggca
     7081 ttgtggaaac agtttgtcga tatttcgctc tcgcacgccc aagccgttta taacacgctg
     7141 ggcttgaagc tgcgccccga agacgtggcg ggcgaatcga agtacaacga cgatttgcaa
     7201 gccgtggtcg atgatttggt tcaaaaaggt ctggcggtcg aagacgacgg cgcgaaagtt
     7261 gtgttcttgg acgagtttaa aaacaaagag ggcgaacccg ccgcatttat cgtgcaaaaa
     7321 caaggcggcg gcttccttta tgcttccacc gacttggcgt gtttgcgcta ccgcataggc
     7381 cgtctgaaag ccgaccgcct gctgtacgtc gtcgaccacc gccaagccct gcacttcgag
     7441 caactcttta ccacttcccg caaagccggc tatctgccgg aaaacgtcgg cgcggcattt
     7501 gtcggcttcg gcacgatgat gggcaaagac ggcaagccgt ttaaaacgcg cagcggcgac
     7561 accgtgaaac tggtcgatct gctgaccgaa gccgtcgaac gcgccaccgc tttggtgaaa
     7621 gagaaaaatc ccgaattggg cgcggacgaa gccgctaaaa tcggcaaaac cgtcggcatc
     7681 ggcgcggtca aatatgccga cttgagcaaa aaccgtacca gtgattatgt gttcgattgg
     7741 gacgctatgc tctcgtttga aggcaacacc gccccctacc tgcaatacgc ctacacccgc
     7801 gtgcaaagcg tgttccgcaa agccggcgaa tgggacgcaa ccgtgccaac cgttttgacc
     7861 gaacctttgg aaaaacaact agccgccgag ctgctgaaat tcgaagacgt gctgcaaagc
     7921 gtggcggaca cggcgtatcc gcactacctc gccgcctacc tctaccaaat tgccaccctg
     7981 ttcagccgct tctacgaagc ctgcccgatt ttgaaagccg aaggcgcaac ccgcaacagc
     8041 cgcctgcaac tggcgaagct gaccggcgac acgctgaaac aaggcctgga tttgctgggc
     8101 attgatgtgt tagacgtaat gtaaaaccgc accgcccgat tgcggacaac agccccgcaa
     8161 tcaaccggcg gaccatcctc atccgaatct gaaaaaagcg gcgcgataca ccgtatccgc
     8221 cgcccctccc aaaatgcgaa acagacaaac gccaagcaag caagcaagca agcaagcaag
     8281 caagcaagca agcaagcaaa aaattataac ccccttcctg ccgacgcacg cactttccgc
     8341 gcggcgcatt ccccttttcc cgcccctcaa acccgccttt tcttcaggca gggtttcagc
     8401 ccgccccctt tccctgtttt cctttccccg acacgcgcgc gcgacccctg ccgcaccgtg
     8461 ccgcactttc gcgcccggac ggcatcgttc cgccatccgg ttctctgttt caaatacccc
     8521 tgtttcagaa agaaattcca gatgcttcaa cacacaggac gacatataaa gcaccgccct
     8581 atgtgttgcc ctgatttgga aggggttgcg cctcccaaat aaagtctgat cctgccgccc
     8641 cgaaggacag atgtccgagt ggcgaagttt caaccgaaaa ggaaatacga tgaatattca
     8701 caccctgctc tccaaacaat ggacgctgcc gccattcctg ccaaaacggc tgctgctgtc
     8761 cctgctgata ctgctggccc ccaatgcgat gttttgggtt ttggcactgc tgaccgccac
     8821 tgcccgccca attgtcaatt tggactacct tcccgccgcg ctgctgatcg ccctgccttg
     8881 gcgtttcgtc aaaattaccg gcgtattggc gttttggctg gcggttttgt ttgacgggct
     8941 gatgatggtg atccagcttt tcccttttat ggacctcatc ggcgccatca acctcgtccc
     9001 cttcatcctg accgcccccg ccccttatca gataatgacc gggctgttgc tgctgtatat
     9061 gctggcgatg ccgtttgtgt tgcagaaagc cgccgccaaa accgacttcc ggcacattgc
     9121 cgtctgcgcc gccgttgtgg cggcagccgg ctatttcacc ggccatttga gttactacga
     9181 ccggggtcgg atggccaata tcttcggcgc aaacaacttc tactacgcca aaagtcaggc
     9241 gatgctctac accgtcagcc agaatgccga ctttattacc gccggcctgg tcgaccccgt
     9301 cttcctcccc ttgggcaatc agcagcgtgc cgccacgcat ctgaacgagc cgaaatctca
     9361 aaaaatcctc tttatcgtcg ccgaatcttg ggggctgccg gccaatcccg aacttcaaaa
     9421 cgccactttt gccaaactgc tggcgcaaaa agaccgtttt tcagtttggg aaagcggcag
     9481 ttttcccttt atcggcgcga cggtcgaagg cgaaatgcgc gaattgtgcg cctacggcgg
     9541 tttgcgcggg tttgcactgc gccgcgcgcc cgacgaaaaa tttgcccgct gcctccccaa
     9601 ccgtttgaaa caagaaggtt acgccacctt tgcgatgcac ggcgcgggca gttcgcttta
     9661 cgaccgcttc agctggtatc cgagggcggg ctttcaagaa atcaaaaccg ccgaaaacct
     9721 gatcagtaaa aaaacctgcg ccattttcag cggcgtgtgc gacagcgagc tgttcggcga
     9781 agtgtcggca tttttcaaaa aacacgacaa gggactgttt tactggatga cgctgaccag
     9841 ccacgccgac tatcccgaat ccgacatttt caaccacagg ctcaaatgca tcgaatacgg
     9901 cctgcctgcc gaaaccgacc tctgccgcaa tttcagcctg cacacccaat tcttcgacca
     9961 actggcagat ttaatccaac gccccgaaat gaaaggcacg gaagtcatca tcgtcggcga
    10021 ccatccgccg cccgtcggca acctcaatga aaccttccgc tatctcaaac aggggcacgt
    10081 cgcctggctg cacttcaaaa tcaaataaca acaatgccgt ctgaacgcac caacagcctt
    10141 cagacggcat tttgcagaca gaccgacccc tcaagcccac tttttcatca tttccgataa
    10201 attgctt
//
LOCUS       DAWKQJ010000062         9890 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000062 DAWKQJ010000000
VERSION     DAWKQJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.062"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL20_001140"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL20_001140"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ7683299.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WJL20_001141"
     CDS             complement(1457..1846)
                     /locus_tag="WJL20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7683300.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRIRLSEVK
                     STLLMNAQTMERYYRQKGTFENYDTNKLKQNKYFKITLSEVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WJL20_001142"
     CDS             2072..3250
                     /locus_tag="WJL20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ7683301.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WJL20_001143"
     CDS             3316..5250
                     /locus_tag="WJL20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ7683302.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5663..6445)
                     /locus_tag="WJL20_001144"
     CDS             complement(5663..6445)
                     /locus_tag="WJL20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ7683303.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6584..8773
                     /locus_tag="WJL20_001145"
     CDS             6584..8773
                     /locus_tag="WJL20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ7683304.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAVQGWIETTEAAKPVLRPYHGQASHSEFVLNANQQKASDAIQTVFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8819..9865)
                     /locus_tag="WJL20_001146"
     CDS             complement(8819..9865)
                     /locus_tag="WJL20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7683305.1"
                     /translation="MLAILGALVFFINPGAIVLHRQIFLELMLPAAYGGFLTAALLDW
                     TGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCARLIWLDRNTD
                     NFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILLGAEALKECR
                     LKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTSLAVGFILLAKLRELHHHEL
                     LRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSVMMVWLTAGL
                     WHSGFTKLDYPKLCRIAVPILFAAAVSRTFLMNVNPIFFITIPAILTAVVFTLYLFTF
                     IPIFRANAFTGDPE"
BASE COUNT         2288 a         2563 c         2900 g         2139 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgcct gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctg gaagcttgcg caggatccaa accttcaggg actttgaccg
      781 cgtaatcggc actcacgatg cagtaggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacaag gtttcgcggc cggtattgca gtattcgcac gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaagatac cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 ggtcggcctg aagggtgaag tggtcggggc tgacttcgct taaggtaatc ttgaaatatt
     1621 tgttttgttt cagtttgttt gtatcgtagt tttcaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttc atcagcaggg tggatttgac ttccgacagg cggatgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcggcga cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaaa cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttac gtcaagctcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagtgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgacttccaa agaggatttg gaaagcgcgc aggatgcgtt
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggcttta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccataagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gcatcgcgtc ggtggtttcc gtcgtcgcat tgggcaacgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca acaccgacct gaccgcctcg ctttacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctagt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tcggcggcat ccgttatcgg ggcggtcgcc
     5161 tgctcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ttgaagctgc
     5281 cggaccggtc attttggagc agaaacttat cggacaaaac ggtttcttag attctacgtt
     5341 ctagattccc acttgcgtgg gaatgacggc ggcgggggtt cgatgattgc acacaacgct
     5401 cgagtcccgt cattcccgta aagacgggaa ttcggttcgt tcggctttgc ttgtttcgga
     5461 taaatcacgg taactcaata ttccagattc ccgcccgcgt gggaacggcg gcggggcttc
     5521 gtattgttca atttattatt ttcaatcatt caatgggtta ggatgtgttt gttggcttgc
     5581 taactttcag ggcggattgg ttttcaggcg gcatttcccg caaaaaaggc ttggaatttt
     5641 ccaagccttt tttgcggatg gattattgat ttttgcggat gatttcctcc agttggggca
     5701 tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc gtgccgttga
     5761 agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag atgctgccgc
     5821 cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg tcgggctgac
     5881 accataagat ttgcgccttg cgcgcggcat cggggtgcag gccggcaatg ggcatcataa
     5941 agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt ttgcagaacg
     6001 gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc acttctttga
     6061 tggctttgtc caaaggcagg gaggcgaagt cgattttgtt caaatcggcg gcgcgttctt
     6121 cggtcaggtt tttgcgcgtg tcgatgttga tgagttcgcc gacgaacata tagccgcctt
     6181 cggcatcggt gtagataatc tgtctgccgc tgacgacgac ttcgtaaatg cctttgaccg
     6241 gtgtttcgct aacgctcaac actttcaaat cttgggcgga ataggttttt tccaaacgcg
     6301 ctttcaaaga ggcggcaacg gatttgccgg cggactcggc tttggcggcg ggttcggcgt
     6361 tggcattgga aacgggcgtt tgcccgcaag ccagcagcgg gaggacggta aagggggtca
     6421 agattttgat taacttggtt ttcatataaa gatgattgcg cgtgttggaa aggcggaatt
     6481 gtatcaaatc tctgttgcgc ctgcattgcg cctaggctca atttatcgtc tgaaaatagc
     6541 ttccgcctgt taaaatacgc aaaaaaatga tttgcctgcc tgtatgatct accaccgcat
     6601 cgccgtaaac gtgccgcttt cagacggcct tttgacttat tcccattccg atccgcttcc
     6661 tccgggaacg cgggtgcttg tgcctttccg caataaaacc gtggtcggga tggtgtggga
     6721 aacggatatt gcgccggata tggatacggc gcggattttg agcgttcgga cggccttttc
     6781 ggacgagccg ctgctgcccg aaagctggcg tgatttgttg gcgtttacgt cgcgttatta
     6841 ccactatccg acggggcagg cggtgtttgc cgcgctgccg cagggtttga aggaaacgcg
     6901 cgcggtggaa atgccgcagc cgccgttgtt ttacgcgctg aacgaagcgg gcagggcgca
     6961 aacgccgccg ccggcgcggt tcaacaaaaa agcggctttg tgggacgcgc tgctgtcggg
     7021 cgaaatgacg atggcagcgt tgaagcaggt aaacgcgcag gcggcgaaat tgattgaaga
     7081 ttgggcggtg cagggttgga ttgaaacgac ggaagcggcg aaacccgttt taaggccgta
     7141 ccacgggcag gcttcgcact ctgaatttgt gttgaatgcc aaccagcaga aagcttccga
     7201 tgccattcag acggtctttg gcagcttcca accgtttctg ctgtatggca tcaccggcag
     7261 cggcaagacc gaggtgtatt tcgatgcgat ggcgaaagtg ttggctcagg ggcggcaggt
     7321 gttgtttctg ttgcccgaaa tcaacctcac gccgcagctt ttgaagcggg tgggaaatcg
     7381 ttttgccgac gtgccgaccg ccgtgttgca cagtcagatg gcggcaggca ggcgcacgca
     7441 ggattatttg cgcgcgatgt tggggcaggc gaaattggtc atcggcacgc ggctggcggt
     7501 gtttacgccg atggatgatg tcgggctgat tgtagtcgat gaggaacacg acggctcgtt
     7561 caaacaagac aacgaattgc gctaccacgc ccgcgatttg gcggtgtggc gggcgaagca
     7621 gggcggctgc cccatcatat tgggcagtgc cacgcccagc ttggaaagct ggcacaaggc
     7681 gcaaagcggc gcgtaccgcc tgctgcaact gaccgaacgc gcccataccg ccgcgcaact
     7741 gccgcaagtg gacattctca acgtaggccg tctgaaactc gacaacggtt tctcgccgca
     7801 agccttgcag cttttgaaac agaattttga agcgggcggt atgtcgctgg tgtacctcaa
     7861 ccggcgcggc ttcgcacccg cgctgttttg cggcgactgc ggccatacct tcggctgccc
     7921 aaactgctcc gccaaaatgg tactgcacca acgcgcccgc caactgcgct gtcaccactg
     7981 cgaccaccgc gaacccatcc cgtacaaatg ccccgactgc ggcaaccaag acctgaccgc
     8041 cgtcggccac ggcacgcagc gcgtcgaaga aaccctgcgc accttcctgc ccaaggcagc
     8101 cgtcgtccgt gttgacaggg acagcaccgc gcacaaaaat gactgggcgg atttgtaccg
     8161 ccgcatcgcc gacaacaaaa tcgacatttt ggtcggcacg cagatgcttg ccaaaggcca
     8221 tgatttcgcg cggctcaacc tcgttatcgt attgaacgcc gacggcagcc tgtacagcgc
     8281 ggactttcgc gcgccggaaa ggctgtttgc cgagctgatg caagtgtcag gcagggcggg
     8341 gcgcgccgac aaacccggca aggtgttgat acagacccaa ctgcccgaac atcctgtctt
     8401 cgccgccgtc aaagcgcaag actatgccgt gtttgccgaa aacgaattga acgagcggca
     8461 aatgttcgcc atgccgccct tcggtttcca gaccgccgtc cgcgccgatg cgccgcgcgt
     8521 tgccgatgcg atggaatttc tcaatgccgc caaagaaacc ctcgccccgc tgttgcccga
     8581 aagcgtttcc cagttcggcg cggccccgat gctgatggtg cgcctcgccg aacgcgaacg
     8641 cgcgcaaatc ttcctcgaat cgacatcccg acaggatttg caccgtgccg tgagtttgtg
     8701 ggtgcaggtg ttgcagcaaa accgcgacgg caaaatcaga tggtcggtgg atgtcgatcc
     8761 gcaggaggct tgattatcgg caatacgatg ccgtctgaaa ccgtttcaga cggcattttt
     8821 attccgggtc gcccgtaaac gcattcgccc gaaatatcgg tataaacgtg aaaagataaa
     8881 gcgtgaacac gacggcggtc agaatcgcgg ggatggtgat gaagaatatc gggttcacgt
     8941 tcatcaagaa agtgcgcgag acggcggcgg cgaagaggat ggggacggca atgcggcaga
     9001 gtttggggta gtcgagtttg gtaaagccgc tgtgccacag tccggcggtc agccacacca
     9061 tcatcacgct gcccatcatg ccaccgaggg taatcaggtg caggggcgcg gaggcgggca
     9121 ggttttgtaa tttcgccgcg cctgtccaca aatagcctgc ggcggcaaag agttggagca
     9181 ggtaataagt gcggacgtag tgtttgcgca ggagttcgtg atggtgaagc tcacgcagct
     9241 tggcaagcag gataaagccg acggcgagcg aggtaaaacc ggcggtttgc gcaggcagcc
     9301 aaagttcggc ggcggcgtgc aggagcagga aggtaatggc gatgttttta tagacgacat
     9361 tggggatgaa tactgggtct ttcagacggc attctttcag ggcttccgcg cccaaaagaa
     9421 tactgacgcg cacggatacg aacatcaccg ccgccatatt tagatgcact tgcgcgcgca
     9481 acaggttcaa atcgccgctg acggcatatg ccgtctgaaa aacagtgaac gcggcaagta
     9541 acattagcag ggcgaagttg tcggtgtttc ggtctagcca aatcagccgg gcgcagaaca
     9601 gcagcaacac cagccaatag gcggcgacga aaaacgaggc agtttgcggc gaaaagggca
     9661 gtatagcgga tgcggcgagc aataatgccg ccatcaaagt cgcgacaggt ttcaggttac
     9721 ccgaaaaacc cgtccagtcc aacaaagccg cagtcaaaaa accgccgtat gccgccggca
     9781 gcataagttc caagaaaatt tggcggtgca ggacgatggc accggggttg atgaaaaaca
     9841 ccagcgcacc gagtatggca agcaccgccg cgccgacgaa aaacggccgc
//
LOCUS       DAWKQJ010000063         9796 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000063 DAWKQJ010000000
VERSION     DAWKQJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.063"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(12..1307)
                     /locus_tag="WJL20_001147"
     CDS             complement(12..1307)
                     /locus_tag="WJL20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7683306.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="WJL20_001148"
     CDS             complement(1433..2320)
                     /locus_tag="WJL20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7683307.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATRR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2320..3285)
                     /locus_tag="WJL20_001149"
     CDS             complement(2320..3285)
                     /locus_tag="WJL20_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7683308.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WJL20_001150"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WJL20_001150"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683309.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="WJL20_001151"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="WJL20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WJL20_001152"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WJL20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ7683310.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WJL20_001153"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WJL20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ7683311.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WJL20_001154"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WJL20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ7683312.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WJL20_001155"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WJL20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ7683313.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8696..>9796
                     /locus_tag="WJL20_001156"
     CDS             8696..>9796
                     /locus_tag="WJL20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ7683314.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2504 a         2597 c         2433 g         2262 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca aacctgcaat acctgttatc
      181 gccatcccgt gtccggaata accttgcgta taaaaaacat tcggggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatatcgcac tccccgcccc acgaatattc gattttgaca
      301 tcggcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgca
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catacagcca tattgttgcg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtggca ataataaagg tgctaacggc aatcgcctta
      541 cgctccaaag gcttgaatat cggattcaaa ccggcatagg tattgacagc atagaccaca
      601 tttttgcact cgacgctgcc ttcgggcgtg taaaccagcc aaccgttttg atgcggttcg
      661 atgcacgtca tcggggattg ctcgaaaatc tgcgcaccgg cttcggcagc ggcacgagcg
      721 atgcccaaag tgtaattgag cggatgcagg tgtccggata aggggtcgaa ttgtgcccct
      781 tggtacatat cgctgtcaag ctgctgtttc aactcggctt tatcccaaag ttgataatga
      841 ctcgcaccgt aatgccgttg ggcgtgttca tgccactgct gcaactcttc ccaatgctgc
      901 ggacggacgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcctgc
     1021 tccaagccga cctgtttttc aatttccccc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctaaaataat aagaaggcag atattcttga aaatcaggac gaatcattat gtttacttta
     1321 tcaggtgtat tttcagacgg catgatatgg tttgccgtac catgccgtct gaacgggaaa
     1381 ttactttaag aaaacaggct gacctgataa tgcggtcagc ctgccctgtc ggtcaatgcg
     1441 ctttctcagc agccagtttt tcctggcggt aggcttctgc cgcttctcgg tcacgcctgg
     1501 tcgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatca
     1561 ggatggttgc caaaacgttc atctgcggat caagacccag cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaattacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 attggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtacgcc atacacagcg
     1861 tcgtatgtcc gaggaagatg gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcagcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat accgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacaggca
     2941 aatcactgtc gtcatcaatg cggttttgat tgaagaccag taggtcatca gatagatatt
     3001 gctgttttca ctgtcgccga acgggttaag ggtatgccaa aaattttgga aaatatcggc
     3061 atagttttga taactgatgg tgatattcag acgacctaaa tcttcgtcga tgctggtcag
     3121 cggcgtaaac ggcggaatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccacacgctg
     3241 ccccgggcgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac agtggcgtag gctggttttc cggccagctg atatagacgg tttcgtccca
     3361 agttggcggt gtaatgttgc gcacatacca gtaaggggcg gggacttggc tcttgacgac
     3421 gcgcccgttg gcgagtttga tatggtatat ggcgaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 gtcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa aaatattggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcggacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgtgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgtt ggcgcagttt
     3901 tttgtccaac gcgccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gttgctgacc gccggacaat tggtgcggtt tgcgtttagc
     4021 aaatttagtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccgaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcgatca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa tttttccctg attgggactt tccatacccg ccagcatacg
     4261 cagcagtgta gattttccgc tgccggaact gcccaaaagg gcgaagattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg
     4381 gatttgcaga taaggcttgg ttgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaagac gagtaccggc aaaacggatg ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gttaaacgtt tgatgccgtc tgaaactctt gtaaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt cttttgggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt ctttgcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatga aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttctttct tcttcggttc tttaccgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccaat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tggatacgac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agcgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcaaaac gcatcgacag gtaaataatg atgccgataa cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccgact tgcgggccga taaattcgac ttggcgcaag
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgta ccgccggtaa attcgacaga gaaattcaga
     6121 cctctggtaa ccaaaaagaa tacggcggca ataaaggtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttacctg ccgcagtttc ggcttcaggt ttccataccg caccgatgga aatattctgc
     6301 aatttgcgtc tgcgtccgta ccacagattg accaacgcac ggaatacgac gacggaggaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc ggcacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tggcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gcctgtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccga aaatgctgcc gcccgcgctg tccaagctca gactgacggc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtctgc acctgactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagtgcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aagtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gacgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc tgcgtttttc
     7921 agcgcggcat ccactttgaa ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaacccttcc cagccaggca agaccggaag gcggcatctt gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggctttcca
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc gttctacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actgttgcgc ctttttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg tgccgggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgccg gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcac ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga ttttccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacactttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccattgc cgaataccgc ctgaaacgca ttgccgtcat
     9241 cgatttcgat gtccactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct tttccccttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcacattccg
     9421 cgaagccgtc cgcagacagt ggctgccccg gcttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct agacgaatcg ggcaggctca acctgcacga
     9541 ggcggatttt gcctggctga cacacaaaat cattcagacg gcatcgggct gccccggcaa
     9601 aatcgtatcc gtactcgaag gcggctacac ccttgaacct ctggcacaat ctgctgccga
     9661 gcatatccgc gtgctggcag ggctgggcaa atccgatgcc gcaaccgcct atcagaaaac
     9721 actggaccca acaaaaaaac ggtttgccaa accgaaaacc ggacaggtgc gccaacctac
     9781 ccaatccgac cgttac
//
LOCUS       DAWKQJ010000064         9644 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000064 DAWKQJ010000000
VERSION     DAWKQJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.064"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..546)
                     /locus_tag="WJL20_001157"
     CDS             complement(<1..546)
                     /locus_tag="WJL20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7683315.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(578..961)
                     /locus_tag="WJL20_001158"
     CDS             complement(578..961)
                     /locus_tag="WJL20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7683316.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1330..2034
                     /locus_tag="WJL20_001159"
     CDS             1330..2034
                     /locus_tag="WJL20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ7683317.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASYGVEGTDSVA
                     PGVEEISISVNGTVQF"
     gene            complement(2099..2665)
                     /locus_tag="WJL20_001160"
     CDS             complement(2099..2665)
                     /locus_tag="WJL20_001160"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ7683318.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2731..3146)
                     /locus_tag="WJL20_001161"
                     /pseudo
     CDS             complement(2731..3146)
                     /locus_tag="WJL20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3208..4107)
                     /gene="rdgC"
                     /locus_tag="WJL20_001162"
     CDS             complement(3208..4107)
                     /gene="rdgC"
                     /locus_tag="WJL20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ7683319.1"
                     /translation="MWFKQISFYPLNKEKLPEADILADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(4138..5595)
                     /gene="der"
                     /locus_tag="WJL20_001163"
     CDS             complement(4138..5595)
                     /gene="der"
                     /locus_tag="WJL20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ7683320.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5749..6378)
                     /locus_tag="WJL20_001164"
     CDS             complement(5749..6378)
                     /locus_tag="WJL20_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ7683321.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQNEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6379..7674)
                     /gene="hisS"
                     /locus_tag="WJL20_001165"
     CDS             complement(6379..7674)
                     /gene="hisS"
                     /locus_tag="WJL20_001165"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ7683322.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7801..8343)
                     /locus_tag="WJL20_001166"
     CDS             complement(7801..8343)
                     /locus_tag="WJL20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7683323.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8515..8706)
                     /locus_tag="WJL20_001167"
     CDS             complement(8515..8706)
                     /locus_tag="WJL20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683324.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8739..9275)
                     /locus_tag="WJL20_001168"
     CDS             complement(8739..9275)
                     /locus_tag="WJL20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683325.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9443..9643)
                     /locus_tag="WJL20_001169"
     CDS             complement(9443..9643)
                     /locus_tag="WJL20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683326.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2259 a         2313 c         2457 g         2615 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgtcgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgttcag gcgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagtagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttgggggagg tactgccatt cgcagccgta tttgtgttgc aggacgtgtg
      661 cgaaatggcg gctgacagcg agcagtgcgg aggcgtgcgc cgctgctttt tccatcggct
      721 gccattggtg gggacggatc agaccgcgcg tgatggtgct gctgtgttcc gtgacgacat
      781 aggggatgcc gtatttttgg gaaatttttt gggcgagtat gccggcatag ttcatgcggt
      841 gggcgtgaat caggtcgggc agcccgtttt cgcggatgta gtgtttgaaa gctttcaaac
      901 ccgcacacac ccagcggatg cagtcgatgt cgatgaacgg aaagcggggg aagaaatata
      961 tgccgtgcca tgcatagatg tccaaaccgc tttgccgata ttttgcaaaa ccgtaaggcc
     1021 ggtcaggatg cttgctgttt ctttccgcaa gtagcggaac atcggtgcaa gcacggcggt
     1081 tttgatgcct ttcctctgca atgccagtgc ctgattttga aaaaaatccc gtccacatcc
     1141 tgttcggatt gcggatacca tgaggggatg acgaggacgt gcaagggttc gggcatagtg
     1201 ggattccgta tcggaaaggc ggttattata agacagacgc agaccgaata tttaaattgt
     1261 tgccttacgc taatgcaatt tagcgcgccg atatgctaga ttggcggttt tatgagtgag
     1321 gatacggata tgttgcgttc tattttggcg gcttccctat tggcggtatc ttttccggca
     1381 gcggctgaag cattgaatta caatattgtc gaattttccg aatcggcggg tgtcgaggtg
     1441 gctcaggata caatgtccgc acgtttccaa gtggcggcgg aaggacggga caaaaatgcc
     1501 gtcaatgccg agtttgttaa aaaattcaac agttttaaca gaaaatcgaa aaacggcagc
     1561 tttaaaaccg aattggtatc gcgcagtgcg atgccgcgct accagtacac caacggcaga
     1621 cgcattcaaa caggctggga ggagcgtgcg gaatttaagg tcgagggtag ggattttgat
     1681 gcgttgaacc gttttattgc cgatgttcag gcagatgccg cgttggaata tacggatttc
     1741 catgtgtcgc gcgaacgccg caacgaggtc atcgatcagg tcagcaagga tgccgttttg
     1801 cgtttcaagg cgcgtgccga aaagttggcg ggcgttttgg gtgcgtccgg ttataaaatc
     1861 gtcaaattga atttgggaca catcggcagc catatcgcgg gtgggggagc tgctcaggca
     1921 aaaatgcttc gtgccatgcc gatggcggca agttacggcg tggagggtac ggattccgtt
     1981 gcacctggtg tggaggaaat cagcatcagc gtcaatggga cggttcagtt ctgatttgag
     2041 gtgaacggta aatgccgtct gaaacccgac gataagggtt cagacggcat tttgtgtttc
     2101 aggctttggg cagggttacg ccggtttgtc ccatgtattt gccgttgcgg tctttgtatg
     2161 aagtttcgca cacttcgtcg ctctcgaaaa acaggacttg cgccacgcct tcgccggcgt
     2221 agattttggc gggcaggggg gtggtgttgg aaaattcgag ggtaacgtag ccttcccatt
     2281 ccggttcgaa cggggtaacg ttgacgatga tgccgcagcg tgcgtaggtc gatttgccca
     2341 agcagacggt caggacgttg cgcgggatgc ggaaatattc gaccgtgcgc gccagtgcga
     2401 aggaattggg cgggatgatg cagcagtcgt cttcaacggt aacgaagttt tttgggtcga
     2461 agtttttggg atcgacgatg gtgctgttga tgttggtgaa gattttaaat tcgtttgcgc
     2521 agcggatgtc gtagccgtag ctggacgtac cgtaggagat gatgcgtttg ccgtcggctt
     2581 ctttgatttg gttcggctcg aaggggtcga tcatgccgaa ttcttcgctc atgcggcgta
     2641 tccatttgtc ggacttgatg ctcataatgt tttccttgtt tcttgcagtg ttcggacaaa
     2701 gcattggggg atgccgtctg aaaacggggc ttatttgttt ttaggcagtt tcacttcttt
     2761 aatcatgccg ttttcgcatt tcataatgaa gagggtttcg ccgttttgcg agacggtaaa
     2821 tctgccgtgt gccaagcctt ttttgaacgt gcccgagagt accatgttgc ggaatttggt
     2881 actgtcggaa ttgaacggtt cgataaatat ttcgcggttg gcggcaacgg tataaacgcc
     2941 ttgtccgtcg aatttgccat ttttaaacga accggtatag ttgcgcccgt cttggcagcg
     3001 catgtgcctt tgcctgaagg aagcccgtct tttccgactc cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggcc gaagtgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ttgacggtat
     3181 tttgtcgtga aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag
     3241 ttcttccaac atggtgctga cggattccgc catcagaatt tgcgaggcga aggcaaggcc
     3301 ggcggcatcg tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg
     3361 cttgagtgtg aagtcttgag tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag
     3421 ttgggtaacg gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg
     3481 tttggatact ttgacgacgg gaacaatatc gcccgtacct ttgagttcgc aatcgctgtc
     3541 taattcaaaa ccgccttcgc aatgcccttg caacagccag ccggtcatca aggaagaggg
     3601 cgattgcttg gtattcggca gcgaggcttc caaaccgccc aaagcttcgc gcagcttggt
     3661 caggatgttt tctgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt
     3721 gttaaacacc gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt
     3781 aatttgctct ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg
     3841 gatttccgct accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc
     3901 ttcttttttc agggcgacgc gcaaggtaaa gtcggcaggg aaaacgagtt caggggagaa
     3961 tgaaaccggt gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc
     4021 agcttcagca agtttgtcgg caagtatgtc cgcctcaggc agcttttctt tgttgagcgg
     4081 ataaaaacta atctgcttga accacataat gtttcctatt gtttgaaatg tcgggaatta
     4141 tttgctgaat tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc
     4201 tttgcggcct tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt
     4261 cttcggtttg tcttccgcat tttcatacgg gttttccgaa acattgtatt gaatccgcaa
     4321 cggcgtgcct tgcaggttga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct
     4381 gtcggaaatc gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc
     4441 ttggtgggca taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc
     4501 gatcgcgctt tgtaatacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt
     4561 gtaggcagcc tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat
     4621 aaagtggaac ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg
     4681 ctcgcgacgc tcttcgctga tgccgtccca tttgttgacg gcaaccacca aggcgcggcc
     4741 tgcttccaaa gcaaaaccgg caatcgtcgc atcttggtcg gcgatgtcct gctgcgcgtc
     4801 caataccaaa acagcgacgt ttgccgcttc aaccgcctgc atcgctttga taacggagaa
     4861 tttttctact gcttcatcca ctttgccgcg acggcgcacg cctgcggtat cgatgatggt
     4921 aaacggtttg ccttcgcgtt cgaaatcgat gtggatgctg tcgcgtgtcg tgcctgccat
     4981 atcgaaagca atcacgcgct cttcgccgag gatggcgtta accagtgtgg atttgccgac
     5041 gttcggacgg ccgatgacgg caaagacggg atgttttgca tcggcttctt cggcttccgg
     5101 ctctgggaag gtttccaaaa tatcttcaat cagataatac acgccatcgc cgtgcgcacc
     5161 tgaaataaca taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct
     5221 gttgccgcct tcgcctttgt ttaccgccag ataaacaggg cgcgggcttt ggcgcaggcg
     5281 gtcggcaatg attttgtctt gcggggtcag gccggtacgg ccgtccacca aaaacacaac
     5341 cgcatcagct tcatcgacag cctgcaaggt ttgttttgcc atttcgtgca aaatgccgct
     5401 gtccacaacc ggctcgaaac ctccggtatc gatgaccaaa taaggtttgc tgccgacttt
     5461 gccgtgtccg taatggcggt cgcgggtcag gccgggcagg tcgtgtacga gtgcgtcttt
     5521 ggtgcgcgtc aaacggttga ataaagtaga tttgccgacg ttagggcggc cgacaagcgc
     5581 gatggtgggt ttcattattg ggtctttctg tgtcaagtgc cgttcgggag aactgaacac
     5641 gagcaggtgt ccgttggaca cggtaaatag gttcttcacg gcaagaggcc ataaagaggg
     5701 agggtaaaat gccgtctgat aaataatgga cagcatttca gacggcattt atttcagcga
     5761 atcgagtttc atttgaacca attcgcgacc gacagaatct tgaggcattt tttctaaagc
     5821 ctgtccgtag ttttttaagg cttcctggct ttttccctgt gcggcataga catcgccttt
     5881 ggtttccatc agcagagggg cgaagtccgc ttcaaccggc gtgtcgagcg cggcaagtgc
     5941 ggcatcgtat tttttttgtt gcaacaacac aacgcccaga cgctgcgccg ccaacgcttg
     6001 aatcaggctg tctttttggt tggacaacac ccatttcaaa tggccttcgg caacatcgta
     6061 acgctgcgcg tcaaattcgg ttgccgccgc catcagcgtg gcttgggcgg cggaaatgga
     6121 atgcgggtag ctttgttgga gtttggtcaa ttcggcattg atttcgtttt gcggggcttt
     6181 gttttgcgcc ttttccacga tgtttgccag caccgccgcc gcttcctgat tttgggaagc
     6241 cgcacggttt tggtaaaccg tgtatcccaa gtagccgagt gccgccaaaa tcagcaaggc
     6301 aaacagccat ttgcccgtgg ttttccaaaa atatttaaag ttgtctaact cttgttgttc
     6361 ttcgagatgg gctgccattt atgcgttctt ccattgttgt aaagtatggg ttaaatcctc
     6421 ggcggcgaca gtttgctgac cgtgtgcgcc gttcatgtct ttgagcgtaa ccgtaccgtt
     6481 cgccagttcg tcttgcgcga caatcagggc aaagcgtgcg ccgctgttgt cggctttttt
     6541 catttgcgct ttcaggctct gatagccgga atgctgcatc acattgaaac cttgcgcgcg
     6601 caaggcttgt gcgtatttca tcacctgcaa gtccgcccct tcgccttggt gcattgcata
     6661 gacatcaggc gcagcgttca cttccagcga gccgtattcg ctcaccaaaa gcagcagccg
     6721 ttcgatgccc atcgcaaagc cgatggacgg cgcaggtttg ccgccaagtt cctcaatcaa
     6781 accatcgtaa cggccgccgc cgcacacagt tgcctgcgcg ccgagtttgt cggtcgtcca
     6841 ctcaaaaacc gtctgattgt aataatccaa accgcgaacc aagcgcggat tttcaatata
     6901 ttggataccc aaaccgtcca acatcgcctt gaagcgtgca tagtggtttt gcgaagcctc
     6961 gcccaaataa tccaccaaac gcggcgcggc gttgcagatt tcctgcaaat cagggttttt
     7021 cgtatctaaa acacgcaaag gattggtttt cagacggcgt ttgctgtctt cgtccaattt
     7081 atcttcatag cgtgtcaaat attcaaccaa cgccgcacgg tgtgccgctc gctcctcacg
     7141 gttgcctaag ctgttgattt ccaaagtcag gtattcgcgg atacccaatt tttcccataa
     7201 gtcggcagac atcgcgatga tttccgcatc aatatccgga ccttcaaaac ccaaagcttc
     7261 gataccgacc tgatggaact gacgataacg gcctttttgc ggacgctcgc ggcggaacat
     7321 cggccccata taccacagct tttgcgggct attgtacaga aggttgtgtt cgaccaccgc
     7381 acgcagacaa gaagccgtac cttcaggacg caggctcaaa ctcaaagaat cgtttgaatc
     7441 ggagaaggtg tacatttcct tgccgaccac atcggtttct tcgccgatgg agcggacaaa
     7501 caaaccggtt tgctcgacaa tcggcgtacg gatttgctga taaccgtaag cgcgtgtcca
     7561 gcggccgacc gtatcttcaa acgcctgcca aaacgcagcc gtcagtttga aatctttttg
     7621 ctcgacaggc agaaggtcgt tcatgccttt gacggattgg attttttgtg ccatttcaag
     7681 taagaatgct taaatcaaat tgagggcgat tatagcggat ttttaagggc ttgtgaggat
     7741 agagatggtt tgcggacggg taaaaatgcc gtctgaaaca gttttcagac ggcatttgac
     7801 ttactctgca cgtgcttgcc tgatttgtcc gactgcaaac tctgtctgcc gcttgggatg
     7861 atgtgtgaaa aacaatttat ttgaaattgt ctcggctttt ttcggcacga ccgccaaaat
     7921 cttacctgcc aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat
     7981 tgaaacatga cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta tcccgcgcaa
     8041 taccgagaaa tgatcatcct tgcggtattt cagatacacg atgacgggga ttttcaactg
     8101 tgcgagctgc tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat
     8161 gcgccgcata tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc
     8221 ttcttccgtc agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc
     8281 acaggaaaaa tccaaatcct gctttacaat attgaaatcc cgccgcgctt tccaactctg
     8341 cactttgatt tttccgtaaa caacaggatt atcgttaaac atcggtgcag cattcaaacg
     8401 ataagataaa gaaacgacaa cacacgccaa cagaaaaaca tatttgaact tcatcatatt
     8461 gtccacataa agggcagcct gaaaatcttt caggctgccc ttgtcaaatt attcctagct
     8521 ttcggctttt ttggcaaacc aaacaatccg attacccgca taatactttc catttattga
     8581 aatccgacaa gccgcgccca aaaaatgcca tgcactgtcg atttccgcag caatctttgt
     8641 accgttttct tcaaattcca aatattcacc caataataaa cttgaaacag aacgcgtggg
     8701 cagcaagtgc ccaccctacg cttattcaaa taatttgatt aaataaagaa taaagaaaat
     8761 gcatcaggaa caaaattata atctgccacc tgactcacac cgcttttaaa gtaaggggca
     8821 tcaaaatcaa aaccgcaaaa aaaataattt ttgcattgat ttttaataga tttaaaattc
     8881 gaatatagtg tttctccaga tttaaaaagt ttatctttat ctatatattt gatgctttcc
     8941 ctatccaaaa taaatatttc aaacattaaa aaatcattac atgaccaagc caaatcataa
     9001 atttgattca aatgggtatc ataaagataa aaataataag gtttgggaac aggtaaaata
     9061 tttaatggaa aaggaggact aattttctta atatctgatg actgatatcc atatctttct
     9121 atattcttaa aaatttcatc tttcttaaga taacaagaca ttctgacaat cacaacctgt
     9181 tcatcggata tattatctat ttgcatggcg cgtataacac gccatgcctg attaaaatta
     9241 gtctccctta ccttagaatc tattatcttt ttcaaagatg aaaatgcccc ttatcttgaa
     9301 ctctccagtt agaacctgga agtttcgtac ctcttaaatg tgtattaata tttcttatag
     9361 tttcattaaa atgccacgcg ctgccgattt caacggtaat tttcgtaccg ctttcttcaa
     9421 attccaggta ttcccccatt agctaacgca aagaagcaga cgccatttcg gcttcgttat
     9481 gataaacccg ccttccgttg atatagactt ccgcccctgt ccgctccaaa ttccaaaaat
     9541 tccgtactga aatttccata tcccgatatt gtgcagacca tgttttttcg aaggttttca
     9601 taaaatttcc tatacctgtc caatcggcac atatcaattg catt
//
LOCUS       DAWKQJ010000065         9495 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000065 DAWKQJ010000000
VERSION     DAWKQJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.065"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            87..296
                     /locus_tag="WJL20_001170"
     CDS             87..296
                     /locus_tag="WJL20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ7683327.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            385..1599
                     /locus_tag="WJL20_001171"
     CDS             385..1599
                     /locus_tag="WJL20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ7683328.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1686..4265)
                     /gene="clpB"
                     /locus_tag="WJL20_001172"
     CDS             complement(1686..4265)
                     /gene="clpB"
                     /locus_tag="WJL20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ7683329.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4526..5536
                     /gene="trpS"
                     /locus_tag="WJL20_001173"
     CDS             4526..5536
                     /gene="trpS"
                     /locus_tag="WJL20_001173"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ7683330.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5604..6149
                     /locus_tag="WJL20_001174"
     CDS             5604..6149
                     /locus_tag="WJL20_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683331.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVVRLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6469..6792
                     /locus_tag="WJL20_001175"
     CDS             6469..6792
                     /locus_tag="WJL20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683332.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7001..8509)
                     /gene="ppx"
                     /locus_tag="WJL20_001176"
     CDS             complement(7001..8509)
                     /gene="ppx"
                     /locus_tag="WJL20_001176"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ7683333.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8626..9357)
                     /locus_tag="WJL20_001177"
     CDS             complement(8626..9357)
                     /locus_tag="WJL20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7683334.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLRLSLGYKNR
                     SVTRSFGIVPFIGQNLLGGSRYNFVGGFNADFSRRLSERWRLTLNVGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTLVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2206 a         2464 c         2508 g         2317 t
ORIGIN      
        1 tttgaatttt tcagaaaatg aagcggacgt ttgggcggag gcaacttgtt tgataagata
       61 gcaatatttt aaaacggaga aagatcatgc cttatgtcaa tattaaagta accggcggca
      121 aggaagcacc gactgccgcg caaaaagcgg aactgatcgg cggcgtaacc gaattgctgg
      181 cacgcgtgct gggcaaaaat cccgaaacaa cggttgtcgt gattgacgaa gtggataccg
      241 ataactgggg aataggcggc aaaagcgtca gcgaacggcg caaagagggc aggtaaaaag
      301 cctgaaaatc tcggtttgat gctttaaatt ccgcgtgaaa aagagtacat tcccacccat
      361 tgcccaaaat ttacggaaca catcatggat aaatttccca agtctgcaaa gctcgatcac
      421 gtctgttacg acatacgcgg gccggttcac aaaaaagccc ttcagttgga agaggagggc
      481 aataaaatcc ttaaactcaa tatcggcaac cctgcgccgt tcggctttga agcccctgat
      541 gaaatcttgg tcgatgtcat ccgcaacctg ccgacttcgc aaggctattg cgattctaaa
      601 gggctgtatt ccgcccgcaa agccattgtt cactactatc agaccaaagg tttgcgcgat
      661 attacggttg atgatgtcta tatcggcaac ggcgtgtccg agctgattac gatgtctatg
      721 caggcattgc tcaacgacgg cgacgaaatc ctgattcccg cgcccgacta tcccttgtgg
      781 acggcggcgg caacgcttgc gggcggtacg gtacgccatt atctgtgcga cgaagaaaac
      841 ggctggttcc ccaaccttgc cgatatggaa gccaaaatca cgcccaaaac caaagccatc
      901 gtcgtcatca atcccaataa tccgacaggc gcggtgtaca gcagggaaat cctgttggaa
      961 atcgccgaac tggcgcgcaa gcacggtttg attattttcg ccgacgagat ttacgacaaa
     1021 atcctttatg acggcgcggt tcaccaccac atcgccgcgc ttgcccccga ccttttgacg
     1081 gtaacgttca acggtttgtc caaagcctac cgtgtagccg gattccgcca aggctggatg
     1141 gtgctcaacg ggccgaaaca tcatgcaaaa ggttacatcg agggtttgga tatgctctcg
     1201 tctatgcgcc tgtgtgccaa tacgccgatg cagcacgcga ttcagacggc attgggcggc
     1261 tatcagagca tcaacgaatt cattttgccc ggcggacggc ttttggaaca gcgcaacagg
     1321 gcgtgggaac tggtcaacca gattcccggc gtatcctgcg ttaaaccgat gggcgcgatg
     1381 tatatgttcc caaaaatcga taccgaaatg taccgtatcc gcgatgacat gaaattcgtt
     1441 tacgatttgc tggtgcgcga aaaagtcttg ctggtgcagg gaacggggtt taattggatc
     1501 aagcccgacc atttccgcat tgttacgctg ccttacgtcc atcagattga agaggcgatg
     1561 ggcaggttgg caagattcct gcaaacctac cgccaataag gggacggttt gtctgccgag
     1621 gataaaaaat gccgtctgaa acggagattc ccgtttcaga cggcattttc aacagcagga
     1681 acgaatcagg caaatttcag tctgtcgccg tcggcttcta ccctgatttc gctttcgggc
     1741 gcatagtttc cggcaagcag ggcttttgcc agcgggtttt cgatttccga ctggatggcg
     1801 cgtttgagcg gacgtgcgcc gtaaatcggg tcgaaaccgg ctttggcgat gatgtccaga
     1861 gcggcatcgg aaacagtcag gcggaggttt tgtttttcca aacgtttttc caagcctttg
     1921 agctggattt tcgcaatgtt gcggatatta tcctgatcca gtccgtggaa cacgaccact
     1981 tcgtcgatgc ggttgatcat ttcaggacgg aaatgttctt tcacatcctc catcacaact
     2041 tcttttaccg cttcgtaatc ctgaatgccc atttgttgga tgtgctggct gccgatgttg
     2101 gaagtcatca caatcacggt atttttaaaa tctaccgtcc tgccttgccc gtcggtcaaa
     2161 cgaccgtcat ccaatacttg cagcaggatg ttgaacacgt cgggatgggc tttttccact
     2221 tcgtccaaaa gaatcacgct gtacggtttg cggcggactt gctcagtcag gtagccgcct
     2281 tcttcgtagc cgacatagcc cggaggcgcg ccgattaagc gggcaacggc gtgtttttcc
     2341 atatattcgg acatatcgat acgaatcaga tgatcttcgc tgtcgaacag aaagcctgcc
     2401 agggctttac acaactcggt tttacccacg ccggtcgggc ccaagaacag gaagctgccg
     2461 taaggcttgt tcggatcggc aagaccggag cggctgcggc ggatagcgtc ggacacggca
     2521 cgcacggctt cgtcctgtcc gaccacgcgg cggtgcaata cttcttccat tttcagcagt
     2581 ttgtcgcgtt cgccttccat cattttggat acgggaatgc cggtcatacg ggaaaccacc
     2641 tctgcgattt cctccgcgcc gacattatta cgcaagagtt tgtttgccgg ttttgtgctg
     2701 tccgtatctg cccgttcggc ggctgcacgc tgtttttcca aatgctccaa atcttcatac
     2761 atcaattttg aagccagtgc caaatcgcct tgccgttttg cctgttcgat tttaattttg
     2821 acttcgtcaa tttgtttctt aatattagca gcaccgtctg aaattgcttt ttcggctttc
     2881 cagatttcgt ccaaatcggc atattctttt tccaaaccgt cgatttcctc atctatcagt
     2941 tccaaacgtt ttttgctggc atcgtctttt tctttttcaa cgtgcgcctt ttccatccga
     3001 agctgaatta gacggcggtc gattttgtcc attgcttccg gcttggtttc tttttccatc
     3061 ttgacacggc tggcggcttc gtcaatcaaa tcaatcgctt tatcaggcag gaagcggtcg
     3121 gtaatgtagc ggtcgctcaa ctccgctgcg gcaacgatag cagggtcggt aatatcgata
     3181 ccgtggtgga tttcataacg ctcctgtaaa ccgcgcaaaa tagcgatggt gtcttccacg
     3241 cttggctcgc caaccaatac tttttggaaa cggcgttcaa gtgccgcgtc tttttcgatg
     3301 tattggcggt attcgtccaa ggtagtcgca ccgatacagt gcagttcgcc gcgtgccaaa
     3361 gccggtttca acatattgcc cgcgtccatc gcgccgtcgg ttttacctgc gccaaccaag
     3421 gtatgaattt cgtcgatgaa aatcagagtg ttgccatcgt ctttcgccaa gtcgttcaac
     3481 acgcctttca agcgttcttc aaattcgccg cggtatttcg cgccggcaat caatgccgcc
     3541 aagtccaaaa ccagtaggcg tttgttgcgc agggattcgg gtacttcgcc gttgacgatg
     3601 cgttgtgcca aaccttcgac gatggcggtt ttacccacgc ccggttcgcc aatcagcaca
     3661 gggttgtttt tggtacggcg ttgcaatacc tgaatcgcac ggcggatttc gtcgtcacgg
     3721 ccgataacgg ggtcgagctt accgtctcgg gcgcgttggg tcagatcgag cgtatatttt
     3781 ttcaaagcat cgcgttggtc ttcggcattg gcatcgttca cgttttgtcc tcctcgtacc
     3841 gcgtcaatcg cggcattgat gttttgttcg gtcgcgccgg cttctttcaa aattttgccg
     3901 gccgcatcgt tctgctgcac caaggcaagc aggaaaagtt cactggcgat ataggcatcg
     3961 ccgcgtttgg ttgcggcttt gtccatcagg ttcaacaccg cctgcaattc gcggctgggc
     4021 agaatgtcgc cgccctggcc ggacactttc ggcaggctgt tcaaatgctg ctgcaaacgc
     4081 tgtttcacct gcggcacgtt cacgcccgca tgagccaaga gcgcggcagc accgctgttt
     4141 tggtcgtcaa gcagggcttt cagcacaaag cccgcttcca gatagctgct gtccgcagcc
     4201 aacgccaaac tctgagcttc tgcaagggcc tgttggaatt tggcggttaa tttgtcgtat
     4261 cgcatttttg tttccttttc aaaatgtccg ctgtcgaagc ctatatgtgc ataattgtgg
     4321 ataactcaag ttctgttttc tgtttttcta tatttaattc gatatatcat tgaatttaaa
     4381 gtatataaaa atgcataata atgtgtataa ctatatcttc ttaatatgga aaagtctgtt
     4441 gtcggctgga tgtaggtggc aaatcgggta taatcggcgc atctttttcc ctttcagacg
     4501 gcattgatgc cgcaaggaca tttttatgag caaaaaacga gttctgaccg gcgtaaccac
     4561 caccggcatc ccgcatctgg gcaactacgt cggtgctatc cgccccgccg tccgcgcggc
     4621 gcaaaaccct gataccgaat ccttcctctt cctcgccgat taccacggta tcatcaaatg
     4681 ccacgagccg gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg
     4741 cggactcgac cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga
     4801 attgaactgg attctgacct gcatcaccgc caagggtttg atgaaccgcg cccacgccta
     4861 caaagccgcc gtgcaggcaa atgcggaaaa cgggcaggaa gaccccgatt tcggcgtgga
     4921 aatgggtttg ttcagttatc cgattctgat gactgccgat attctgatgt tcaacgccaa
     4981 cgaagtcccc gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acatcgccgg
     5041 ccgcttcaat caccgcttcc gagaactctt caccctgccc gaagtgaaaa tcgacgaaaa
     5101 cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaacaccat
     5161 tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca tcaccaatat
     5221 gaaagagcca ggcgagccga aacagcccga cgaaagccca ttgtttgaaa tctacaaagc
     5281 cttctccacg ccgtctgaaa cggtggaatt tacgaaaatg cttgccgacg gcctggcttg
     5341 gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc gaactcgccg aaccgcgcga
     5401 acgctacaac gagctgaccg ccgacccttc gcaaatcgaa gagattttgc aggcaggcgc
     5461 ggcgaaagcg cgtaaagaag cacgcgaatt gttggacaaa gtacgcgatg cggtcggcat
     5521 ccgcccgttg aaataaatcc atgccgtctg aacatccatt gtccgtgttt cagacggcat
     5581 tttgaaacca tcaggagtgt ggaatgctga aaacatcttt tgccgtattg ggcggctgcc
     5641 tgctgcttgc cgcctgcggc aaatccgaaa atacggcgga acagccgcaa aacgcggtac
     5701 aaagcgcgcc gaaaccggtt ttcaaagtca aatatatcga caatacggcg attgccggtt
     5761 tggatttggg acaaagcagc gaaggcaaaa ccaacgacgg caaaaaacaa atcagttatc
     5821 cgattaaagg cttgccggaa caaaacgtcg tccggctgac cggcaaacat accaacgact
     5881 tggaagccgt cgtcggcaaa tgtatggaaa ccgacggaaa gggcgcgcct tcgggctggg
     5941 cggcaaacgg cgtgtgccat accttgtttg ccaaactggt gggcaatatc gccgaagacg
     6001 gcggcaaact gacggattac ctgatttcgc attccgccct gcaaccctat caggcaggca
     6061 aaagcggcta tgccgccgtg cagaacggac gctatgtgct ggaaatcgac agcgaagggg
     6121 cgttttattt ccgccgccgc cattattgag gtattcggac atcccggaat atatttgggt
     6181 ttttcaaacc ttgcgaaaaa agtccgcacc gtcgggaaac tcaaaaggag ggggatatgt
     6241 gttacaattt tccgaactgt tttcataaaa tagttttcgg acgtgtttca atatggcatt
     6301 gatgccgcct tattgttcga gaaaaaaaac ctttatattt aaatataatg ggttttaact
     6361 aaacgggaaa ccgttttctc tccggccgat gggcaaaatc agccgattga tggaacacgg
     6421 tccgattttt aagcaaaacc ttaatcaaca actcaagaag gataagacat gaaaaaatta
     6481 ttgattgccg caatgatggc ggctgccttg gcagcttgtt cgcaagaagc caaacaggag
     6541 gttaaggaag cggttcaagc cgttgagtcc gatgttaaag acactgcggc ttctgccgcc
     6601 gagtctgccg cttctgccgt cgaagaagcg aaagaccaag tcaaagatgc tgcggctgat
     6661 gcaaaggcaa gtgccgagga agctgtaact gaagccaaag aagctgtaac tgaagcagct
     6721 aaagatactt tgaacaaagc tgccgacgcg actcaggaag cggcagacaa aatgaaagat
     6781 gccgccaaat aatttgttgc cttggcaaaa atgatgggat gccgcctgcc ggcaacccaa
     6841 acgaaaccgc ctgaagattt tcaggcggtt tttgggttat gtgccccttg ttttttacgc
     6901 ctgcatgacc gttccgagca gatgtgccgc gcatatcggg atgttgcgga caggcaaatg
     6961 ccgtctggac agggtttgga cggcatttgt tccgtaaggt tcagacggcc tcgactttaa
     7021 acggcatatt gattttttgc cattggacgc tttcatattc caatgcgtcg gcaatcaggg
     7081 ggtggcgttc cagccattcc ctgtcaatac gcaggatgaa gccgcagctt tccgtatccg
     7141 tgcgcaactg catgtttttc gggaaagaca ggtcttggcg cgaacggcag aacagtgcgg
     7201 caaggcgcag cgacaaaacg gcataccaca acatttcgtt ggtgccgatg atgccgctca
     7261 tttttttcat atcgccgcga tgaccgatga ccagttgggc aagtatggtc tgttctttgc
     7321 gtgagaaacc cggcatatcg gcgttttcga ggatgtaggc ggaatgcttg tgatagccgg
     7381 tgtgggcgat gtccaaaccg atttcgtgca gcgcggcggc gcgtccgaga tactgttgcc
     7441 acaaggcaag ctcttgaacc gtaacatttt tagcgtggca gaggctgtcc ataaaggttt
     7501 gcgcggtctc ggcggtgcgt ttcgcctgat tgaggctgac gtggtagcgg tgttggaact
     7561 cggcaaccgt ttgtccgcgc atatcttcgt ttaaaccgcg cccgatcaaa tcgtaaaaca
     7621 cgccgtcgcg cagggcggct tcggttacgg tcatcctgtc gagtttcatt tcttcaaacg
     7681 ccgccatcat cacggcaagt ccgccggcaa aaacttcgat gcgttccggt ttcaggtttt
     7741 caaatttggc ctttttgacc gaaccggctt cgatgatgcg ttcggcgagg gcgcgcatgc
     7801 ctttgtaggt aatgtccgcc tcttggggca tttcggcggc aagcacgtcg cggatggatt
     7861 ttgccgaacc cgatgtgccg acggcgaaat cccaaccttc gcgcctcata tttttgctga
     7921 tgcgctggat ttcgttgcgg gcggcggaaa tggcagattg gaagtctttg gcggtgattt
     7981 tgttttggaa gaagcgcagg ctgtaggtta cgcagcccaa gggcaggctt tcagtaatgt
     8041 cgggattcag cgtcgagccg atgacaaatt ctgtcgaacc gccgccgatg tcgataacca
     8101 gcattttgcc gccgcccggg gggagggtgt ggatcacgcc ggtataaatc agccgcgcct
     8161 cttcgcgccc ggcgatgatt tctatgggga aacccagtgc cgcttcggcc ttgggaagga
     8221 aatctgcgat gtttttggcg acgcggaagg tgttggttgc cacggcgcgc acctgttcgg
     8281 ggcggaagcc gcgcaggcgt tcgccgaatt ttgccaggca gtccaaagcc tgttcttggg
     8341 aagcggcact caggtttttc tgttcgtcca gaccggcggc gaagcgcacc atctgtttga
     8401 acgaatcgat aactttcaac tgtccgttgt tgttttcgca aatctggagg cggaaactgt
     8461 tggaacccaa atcgacggag gcgaggatgt ttgcgggggt ggtggtcatg gcggatatcg
     8521 gtgggggaaa aacgcaatgt tactctgacg gcgcaggcgt tgacaataaa tgatgcggct
     8581 gtttttgatt ctgctcgcgg atgttgccga cggcattttt tgcgcttatt tgaaatcctt
     8641 ttccacgctc atgaaaatct gcatgttttt gcgtgtgtaa aaacttttca tattgctgtc
     8701 gattttcaga tagcggaaat tgagttgcgg cgtaaagccc ttccaagaga ttttgtcgtg
     8761 ccacaacgac aggtttgcct gatattcgtg gtctttgcgc gggaagcggt acacaagggt
     8821 cccgggtgca tcaaaagtcc tgcgggtgta gcgcaggttt gcccgcagac ccaagccgcc
     8881 gtcgaacgtt ttgaccgctc cgacacgcaa acccttgcgg atggaagcct gttccgcctc
     8941 cttcgtcaag tcgtgcgacc agtccgcacc gccgtaaagc agccagtctt tcggcgcgga
     9001 atacatcagc gtcgcgcccg ccagcggcat atggctgtcg tatcgggcgg cggtgcggtc
     9061 ttcctgataa cgcttccaca tattgcccac gttcagcgtc agccgccagc gttcgctcaa
     9121 gcgtcgggag aaatcggcat tgaagccgcc gacgaaattg tatcggctgc cgcctaagag
     9181 gttttgcccg ataaacggca cgatgccgaa cgagcgcgtt accgaacgat ttttatagcc
     9241 gagcgacagg cgcaggcact gttcgctgaa atctttgtta tcccaataat gcacgccgcc
     9301 gccgctgatg ccgccgtaga ggaaatgatg cccgcccgca ttgatttcgc gcgacacgcc
     9361 taagccctag cgcaaaccgt gtgccttttg cggcaggctg tcggcggttt tcgtccagct
     9421 tctgcccgca aattcaatcg tttttttgga cgaagcgttg tttatagcgg attaacaaaa
     9481 atcaggacaa ggcga
//
LOCUS       DAWKQJ010000066         9429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000066 DAWKQJ010000000
VERSION     DAWKQJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.066"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..1577)
                     /gene="aldA"
                     /locus_tag="WJL20_001178"
     CDS             complement(135..1577)
                     /gene="aldA"
                     /locus_tag="WJL20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ7683335.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVVAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1612..1812)
                     /locus_tag="WJL20_001179"
     CDS             complement(1612..1812)
                     /locus_tag="WJL20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683336.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1821..2210)
                     /locus_tag="WJL20_001180"
     CDS             complement(1821..2210)
                     /locus_tag="WJL20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683337.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2418..3335)
                     /locus_tag="WJL20_001181"
     CDS             complement(2418..3335)
                     /locus_tag="WJL20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683338.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFAGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3508..4308
                     /locus_tag="WJL20_001182"
     CDS             3508..4308
                     /locus_tag="WJL20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683339.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4356..5132
                     /gene="mlaE"
                     /locus_tag="WJL20_001183"
     CDS             4356..5132
                     /gene="mlaE"
                     /locus_tag="WJL20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ7683340.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5183..5677
                     /gene="mlaD"
                     /locus_tag="WJL20_001184"
     CDS             5183..5677
                     /gene="mlaD"
                     /locus_tag="WJL20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ7683341.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5714..6304
                     /locus_tag="WJL20_001185"
     CDS             5714..6304
                     /locus_tag="WJL20_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ7683342.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6355..6633
                     /locus_tag="WJL20_001186"
     CDS             6355..6633
                     /locus_tag="WJL20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ7683343.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6630..7457
                     /locus_tag="WJL20_001187"
     CDS             6630..7457
                     /locus_tag="WJL20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ7683344.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7582..7944
                     /locus_tag="WJL20_001188"
     CDS             7582..7944
                     /locus_tag="WJL20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ7683345.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7941..8327
                     /locus_tag="WJL20_001189"
     CDS             7941..8327
                     /locus_tag="WJL20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7683346.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8324..8812
                     /locus_tag="WJL20_001190"
     CDS             8324..8812
                     /locus_tag="WJL20_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7683347.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKMANYYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8895..9062)
                     /locus_tag="WJL20_001191"
     CDS             complement(8895..9062)
                     /locus_tag="WJL20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ7683348.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9029..9409)
                     /locus_tag="WJL20_001192"
     CDS             complement(9029..9409)
                     /locus_tag="WJL20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ7683349.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2177 a         2623 c         2380 g         2249 t
ORIGIN      
        1 ttaaatttaa tccgctatac aaagacaaaa acatcgacct cggtcatgac tgattgccgg
       61 tgaagcaata aaaatgccgt ctgaacctga aaacgggttc agacggcatt ttctatcggg
      121 gttttaaagc ggcattaaat gtcggtttcc aaataaacga cttgggtttg cagatattct
      181 tccaaaccgt gtttgccgtc cgcaccgccg atgccggatt ttttccaacc ggcgtggaaa
      241 ccctgcatcg cttcaaagtt ttcgcggttg atgtaggttt cgccgaattg caggcggcgg
      301 gtaacgtaga aggcttcgtt taaattagtc gtataaacag aactggtcag accaaactcg
      361 caatcgttag ccagtgcgat gacttggtcg agcgtgtcga aagtggaaac aggcaggacg
      421 gggccgaagg tttcttcttt cataatgtcc atactgttgt cggtgtcggt cagcagggtc
      481 ggctcgaaga aatagccgcg tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct
      541 tgtttgactg cccgttccac tttttcggca acggctttga cggcgcgctc ttcaatcagc
      601 ggacccattt ccagcgcgcc ggcttcggct tcggcagggt tgccgtagcg cacgcctttc
      661 atcgcggcgg tcattttttc aatgaatgcg tctttcaggc tgctgtggac atagacgcgc
      721 tcggcgcagt tgcagatttg accggtgttg ccgacgcgcg aagccaagat ggatttcact
      781 gccaaatcca aatccgcgtc tttcaaaacg atggcagggg ctttgccgcc gagttccaac
      841 gaaactttgg tgatgttggc ggaggcggct tccatcactt ggcggcctgc ttcgacggag
      901 ccggtcaggc tgaccatatc gacttgcgga tgggcggaca aggcattgcc gatttccgca
      961 ccagggccgt tcactacgtt gaacacgcct gcgggcagtc cgaccgcatc gacgatttcg
     1021 gcgaagatgt ggcagttgat cggggttacg ctgctgggtt tgacgacgat ggtattgccc
     1081 gttaccaaag ccgggcccat tttgcgggca atcaggaaga aggggaagtt ccacggcaaa
     1141 atgcctgcca cgacgcccag cggacgtttg aacaataaaa tgttttcgcg cgggcggtcg
     1201 ctttggatga tttcgccttc gtaacggcgc gcccattcgg cttggtaatc gagataatcg
     1261 gcagtgaaca tgacttccac gcgcgccaag tctttggttt tgccgccttc ggcaacgatg
     1321 gtgtcggtca gctcgtcggc gcgttcgcgt atgccttggg cgattttgcg caaatacgcg
     1381 ccgcgttcga ccgcaggcag acgctcccaa gccggttgcg ccgcacgcgc cgccgcgacg
     1441 gcgcggtcaa cgtccgcctt gccgcctttg ggttcgcggg cgatggtttc ttcggtggat
     1501 gggttcaata cgtcgcgcca ttcgccgttg aaatcgtttt caaagcgtcc gttgatgtac
     1561 atggccaatt gtttcatttc gggttctcca gttttgtagt cagatgtagt cttagtttat
     1621 tcccaaataa attgccgtac aagtttcttt tgaaatgttt agacgagaag ctactatgtc
     1681 tgcatattgt tctgctaatt tatattcact cgaatgcaac tgccgattat tccaatctgc
     1741 attcgcatca gtaaaagccc cagcattgcc gcctgtggca taaccaatcg ctaaactgcc
     1801 aacggtatcc acaacaggtt tcaggctgcc tgaatttctc taataaatga aacacttatt
     1861 ctatcagccg ttaatgtaac caaacaatta tcaagataag agtagtttat taaatactca
     1921 tcttcttcaa attgtatatt ttgtagggtt agatgattat tttttaattc tgccgttatt
     1981 tcatacttga aactatcatc tatttttcta aatccaatat ttgaagttga tttatccagt
     2041 atgtaatcat taaagatttc ataggataat tccacttcat aaaattgatt ttcaaatatt
     2101 ttgaatgggc agtacgatat aaaacaatgt agttcataac catttatttc aatcaatact
     2161 gcctcttcaa tggtgttatc taactttttg attttacatt tatataacat ttatcttgcc
     2221 tttggataaa ttgttttgtt ttttggattg ttattcaaag ttctccaaaa gtcttttcct
     2281 gattttgtat gtatcgtggt aatactacca ttttcattgg ttccaacaaa aataaattta
     2341 gattgacctt tggaatcagt acccaaaaac tgaacatatc cagacacgcg gatttcgggt
     2401 tttaaatcaa atgcggctta ccggttttct tttttggttt ccgcaggcgt gctgccgaaa
     2461 tattgcctga atgcctgagt aaagctggaa acgtggcggt agccgcataa atatgcggtt
     2521 tcgcctatgc ttttgccccc gttttgcaac agataaagcg cgtgctgcat ttgtttgtgg
     2581 tgcagccact cgcttgtggt aatgccgaaa tggtcgcgca tacggcgttg cagcgtcctt
     2641 tcgctgatgt tcagcgcgtc tgtcagccgg ttgacttggt gtgcgccgcc ggcaaacgcg
     2701 gcattcaggg tgcggctgaa gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc
     2761 gccgccggcc cgatgccgtc tgaaaccgtg tcccacaagt ccgacagcag ccgcaacacg
     2821 tccgcctcgc ggcgcaatgt ttcgcccaaa tgcccctttg ggacggcttt caggcaggat
     2881 gccgccaagc cgcgcaggtt cgggggcaaa tcccatatcc tgaccggttc gcggtaaagc
     2941 aggggtgcga aacgcgcgta ttccggacgc agcagccatt gttccatacc tttaatggtc
     3001 attttgaccg ttttgccgcc tcggtaaaga tagcggctga acaggacttc ttccccgaca
     3061 gcaattagga caatcttgcc gccgtccgca tcgatttgga agcggcagcg gttgatgccg
     3121 aagtccaaac tgccttccag caagagcacg aacgacacat aaggttcggc gaggcggctg
     3181 ctgcaaaaat cacaccgtgc cgttactgtg ccgccgtgga tggaaatgcc gttggacaaa
     3241 gtatcgaaac ggtagctgcc ctccacatag ctgccgccgc cgcttttgac tagattgacg
     3301 aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag gttatcattt
     3361 gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaacggca ttttcacaac
     3421 atttaccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct taaaatacac
     3481 gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga aatgaaagac
     3541 gtcgccttcg cgtatggcga ccgcccgatt ctgaagaaca tcaatttcag cattccgcaa
     3601 ggcaattttg ctgccgtaat gggcggttcg ggcagcggca aaaccacgct gatgcgactg
     3661 attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg ggatttggcg
     3721 ggcttttcgg ctgacgagtt gtatgaacac cgccgccgta tgggcgtatt gttccaacac
     3781 ggcgcgctgt ttactgattt gtcggtattc gacaatatcg cttttccgat gcgcgaactg
     3841 acgcgcctgc cggaagcggt gattcgggat ttggttttgt tgaaattgaa cgcggtcggt
     3901 ctgcgcggtg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc
     3961 gcgcttgccc gcacgattgc gctcgaccct gaaattatgt tgtacgacga gccgtttacc
     4021 ggcctcgatc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt caacaaggct
     4081 ttgcgttcga ccagtattat ggtaacgcac gacattgaaa aatctttgga aatcgtcgat
     4141 caggtgattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca ggaaatgcgc
     4201 gaactggatt cgccttgggt gcgccagttt gtcggcgggc tggcagacgg ccccgtagca
     4261 taccgttatc cggcgcaaac gtcgttgcag caggacctgt tgggataaat gagttgatgc
     4321 cgtctgaaac cgttgaactt tacacataga aatttatgaa ctttatccgt tccgtcgggg
     4381 cgaaaaccct cggccttatt caatccctcg gcagtatcac gctgtttctg ctgaacattc
     4441 tggcgaagtc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa gtgtatttcg
     4501 ccggtgtgct gtcggtgttg attgttgccg tttcggggct gtttgtcggc atggtcttgg
     4561 gtttacaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg
     4621 tcgcggcttc gctattgcgc gagctgggtc cggtattggc agcgattttg tttgccagca
     4681 gcgcgggcgg tgcgatgacc agcgaaatcg gtctgatgaa aacgaccgaa cagctcgaag
     4741 cgatgaatgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc ttttgggcgg
     4801 gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggcatt ttcggtgcgt
     4861 atttggtcgg tgtaagctgg ctgggcttgg acagcggcat tttctggtcg caaatgcaga
     4921 acaacatcac gatacattac gatgtaatca acggtctgat caaatccgcc gcgttcggcg
     4981 tggcggtaac gctgattgcc gtgcatcagg gcttccactg tgtcccgact tcggaaggca
     5041 ttttgcgcgc cagcacgcgt acggtggttt cgtccgccct gacgattttg gcggtcgatt
     5101 ttatattgac cgcgtggatg tttacagatt gacagggcgt ctgaagacga aacgcggaac
     5161 atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg tcggactgtt
     5221 cgtcctgatt ggcgcggcgg cggttgcctt tctcgctttc cgcgtggcgg gcggtgcggc
     5281 gttcggcggt tcggacaaaa cttacgccgt ttatgccgat ttcggcgaca tcggcggttt
     5341 gaaggtcaat gcccccgtca aatccgcagg cgtattggtc gggcgcgtcg gcgctatcgg
     5401 acttgacccg aaatcctatc aggcgagggt gcgcctcgat ttggacggca agtatcagtt
     5461 cagcagcgac gtttccgcgc aaatcctgac ttcgggactt ttgggcgagc agtacatcgg
     5521 gctgcagcag ggcggcgaca cggaaaacct tgctgccggc gacaccatct ccgtaaccag
     5581 ttctgcaatg gttctggaaa accttatcgg caaattcatg acgagttttg ccgagaaaaa
     5641 tgccgacggc ggcaatgcgg aaaaagccgc cgaataaacg tatgccatca tattcaaact
     5701 aggacactta atcatgaaaa aatcctcctt catcagcgca ttgggcatcg gtattttgag
     5761 catcggcatg gcatttgccg cccctgccga cgcggtaaac caaatccgtc aaaacgccac
     5821 tcaagtattg agcatcttaa aaagcggtga tgccaacacc gcccgccaaa aagccgaagc
     5881 ctatgcgatt ccctatttcg atttccaacg tatgaccgca ttggcggtcg gcaacccttg
     5941 gcgcaccgcg tccgacgcgc aaaaacaagc gttggccaaa gaatttcaaa ccctgctgat
     6001 ccgcacctat tccggcacga tgctgaaatt aaaaaacgcc aacgtcaacg tcaaagacaa
     6061 tcccatcgtc aataaaggcg gcaaagaaat catcgtccgc gccgaagtcg gcgtacccgg
     6121 gcaaaaaccc gtcaacatgg acttcaccac ctaccaaagc ggcggtaaat accgtaccta
     6181 caacgtcgcc atcgaaggcg cgagcctggt taccgtgtac cgcaaccaat tcggcgaaat
     6241 tatcaaagcg aaaggcgtgg acggactgat tgccgagttg aaagccaaaa acggcggcaa
     6301 ataaccgaca aaccataccg tccgagggcg gtatgggttt tccgagcaaa cgctatgcat
     6361 acagaactca aaaacggaat attgcacatc ggcggcgaca tcaccgtcaa aaccctgacc
     6421 gccgccgctt ttacccgctt cagacagcaa tgtcgtctga aagacacctg cgcggttgat
     6481 ttgagcggcg tcggacgcgc tgattccgcc tgcgtgtcgc tgctgctcga agtgctgcgc
     6541 ggttgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc gctgtccgaa
     6601 ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc ttcctcctgc
     6661 tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac ccttatgaag
     6721 gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt ttcgcccctg
     6781 ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg tccaattttt
     6841 ttaacaacct gtgcgacgtg gtcagcttcg gtagcaatat cttgcgcttg gacatcaaac
     6901 gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg ggcgggctta
     6961 tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc gacacgtttg
     7021 cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg ccgtccaccg
     7081 tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat atcgtcttcc
     7141 gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg cgcgaaggtc
     7201 tgctcgattt gaccgacagt ctggacgaag ccgccatcga caaatacagc tacacgcgcg
     7261 acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa ggtacggaag
     7321 ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg gaaactgccg
     7381 ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc gaaacgcaac
     7441 ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacga cattcccttc
     7501 tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt gcgcataccg
     7561 cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt cctcatccct
     7621 ttagaacctt tttggaactg gctgcaaacc ctgccgggca accacctcga cggactgact
     7681 cttgaagaca tccaagccga cgccaattcc taccttgtcc gcccgtgcga aaccgccgac
     7741 gaagtatggg acgaaatcga agcccgtttt gaagacattt tcgccgccga acttgccgac
     7801 tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa cgaatggttc
     7861 gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc ccgcgaagcc
     7921 ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat ctcgacggct
     7981 acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc tgggcgtttt
     8041 ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc gcccgcatcg
     8101 acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca tgccgcctga
     8161 aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca aacggcaaaa
     8221 ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa cgcaccgtca
     8281 gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa aaatactcac
     8341 cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc ccgacagcaa
     8401 aaccgcgccc gccttctccc tgcccgacct gcacggaaaa accgtttcca acgccgacct
     8461 gcaaggcaaa gtaaccctga ttaatttttg gtttccctcc tgtccgggtt gtgtgagcga
     8521 aatgcccaaa atcattaaaa tggcaaatta ctataaaaac aaaaacttcc aagtccttgc
     8581 cgttgcacaa cctatcgatc cgatagaaag cgtccgccaa tatgtcaaag actacggttt
     8641 gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg gcacacaggt
     8701 ttatccgact tccgtcctta tcggcaaaaa aggcgaaatc ctcaaaactt atgtcggcga
     8761 acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcacaat agcgatgccg
     8821 tctgaagcgc aaaaaaaaca gaaacgatgt tcctgttttt tcagatcatc cgccccgaca
     8881 cgttcagacg gcattcaacc ttccggcaga ttctttttca ccactgccgg attgcctgcc
     8941 gccaaagagt agggcggaat gtcttttgta accaccgcgc ctgcgcccac gaccgaaccg
     9001 cgtccgacgg ttacgcccgc cattacaatc accctgtgcc ccggccagac atcgtcctcc
     9061 aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt ttttgaacgg tcaaacttgt
     9121 ggttatttga ataaaacaga cattccggct ccatcatcac atttttgccg accaccagcc
     9181 cacggcagat ttcacagttt gccccgatgc ccgagccgtc gcccaaaacc gtatccggaa
     9241 acacatacgc cccgcgttcg atattgaccc cgcgtccgat atgcggagaa acccgccgcg
     9301 ccaaaaaacc gcgcacccgt ctgccgacga tgccgatgcc gcgcatatgt gacggcggca
     9361 accccgaacc ggcaaaacgc agcagcagga aaaagaattt tgatcccatc aaacctccct
     9421 caaaagaac
//
LOCUS       DAWKQJ010000067         9383 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000067 DAWKQJ010000000
VERSION     DAWKQJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.067"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(100..297)
                     /locus_tag="WJL20_001193"
     CDS             complement(100..297)
                     /locus_tag="WJL20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683350.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
     gene            complement(294..1349)
                     /locus_tag="WJL20_001194"
     CDS             complement(294..1349)
                     /locus_tag="WJL20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7683351.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1455..1937)
                     /locus_tag="WJL20_001195"
     CDS             complement(1455..1937)
                     /locus_tag="WJL20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7683352.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1984..3120)
                     /locus_tag="WJL20_001196"
     CDS             complement(1984..3120)
                     /locus_tag="WJL20_001196"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ7683353.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3117..3662)
                     /locus_tag="WJL20_001197"
     CDS             complement(3117..3662)
                     /locus_tag="WJL20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ7683354.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTHQTGIRANT
                     QTDFSKFKGCLKDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3659..5134)
                     /locus_tag="WJL20_001198"
     CDS             complement(3659..5134)
                     /locus_tag="WJL20_001198"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ7683355.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            complement(5245..5610)
                     /locus_tag="WJL20_001199"
     CDS             complement(5245..5610)
                     /locus_tag="WJL20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683356.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5631..7541)
                     /locus_tag="WJL20_001200"
     CDS             complement(5631..7541)
                     /locus_tag="WJL20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7683357.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIIQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKVQELAVEAEHNRLFDKFHAQEEAWIRKGIKA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELNPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7560..8204)
                     /locus_tag="WJL20_001201"
     CDS             complement(7560..8204)
                     /locus_tag="WJL20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7683358.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8368..9186)
                     /gene="opc"
                     /locus_tag="WJL20_001202"
                     /gene_synonym="opcA"
     CDS             complement(8368..9186)
                     /gene="opc"
                     /locus_tag="WJL20_001202"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ7683359.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2043 a         2036 c         2665 g         2639 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaactgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaagggat ggttaaggaa
      121 agttatgccc cagataagga tggttgcgcc gacggcttca gacggcattt tggcggcggt
      181 gttgggtttt gtatccggtt tgccgttgtg tcttgtgatg atgattttgg gcgcggtttt
      241 tctgttttga tgtgtgaaat gccgtctgaa aggcggttca gacggcatag cggtcatttt
      301 tgtgcggtca ggcggtcgaa tatgccgccg tcggcgaagt aggttttcat gatgttgtcc
      361 catccgccga atttttcttc gggagagaat gagtctaagt ctgggaagtc ggctttgtgt
      421 cttgccaata cttcggggtt gcgggggcgc aggtagagtg aggcggcgag ttcttgcgcc
      481 ggttcgctcc aaaggtattc gagataggcg cgggcggttt tttgcgtgcc ttttttcgcg
      541 acgacgctgt tgacgacggc gacggggctt tcggcggaaa tggtgtagct cggatagacg
      601 atttcaaact gtccttgggt cagttttttg ctgacgtagt tggcttcgtt ttcaaaagtg
      661 atgagtacgt cgccgatgtt gcgttgtgtg aaggtggtgg tggcggcgcg tccgccgttt
      721 tcaaaaacgg gggtgttttt gaggatggat gcgacgagtt tttgggcttc ctgttcgttg
      781 ccgttggtgg ttttcagacc gtaaccgtat gcgccgagga aggcgtagcg tccgttgccc
      841 gaggttttgg gattggcgat gacgatgtta acgccgtctt tggcaaggtc gttccaatcg
      901 cggatctgtt tggggttgtt ttttcggaca aggaaaacca tagtgctggt gtagggcgcg
      961 gcgtggtcgg ggagggcttg ttgccagcct ttttctacca gtcctttttt ttcgagcagg
     1021 tcgatgtcgg aggattggtt catggttacg acatcggctt gaaggccgtt ggctacggat
     1081 aatgcctgtt tgctggagcc gccgtgggac tgttggatgc tgacggatgt gccggggtgt
     1141 tcggattggt atgttttgat aaataagggg ttgtattctt tgtaaaaatc ccgtgccaca
     1201 tcgtatgagg cgttgagcag ggtaatgttt tttccgtcgg attcggtatt ggccggggca
     1261 ttttgtccgg acggatgatt tgaatcggct gcggggctgc aggcggtgag cagggctgcg
     1321 gtatagagtg ccggtgcgta ggttttcata tgcttgtcct gtcggttggt agatggggca
     1381 actttatacg gctgtctgcg cttgtggaaa taatgtttga tttgaagatt atcagttttg
     1441 gttataagga cggatcagag gtgtttccgc atcagttcgc atttgatttt gatgctgggg
     1501 tcaagctgca atactgccga accgagcgat tcgtagcggt tgagaaggta aaacgggacg
     1561 gagttgagcg atgcgtagag tttcagaaag ctcaaaccgg atttgtgggc gatggtttcc
     1621 gcctgatgaa gtagggcagt gcccagtccg aggttgtgga acagggggtg gacgtaaagc
     1681 gcatcgagtt gtgcttcttg gcagtcgatt tggaaaaatc cctgtatgtt gcctttgtat
     1741 tcggcaaccc aaagtgcttt gtcgggatcg gaaatggtcg gcaggtagct ttctgtgttt
     1801 agcaagcctt cccatacttt tagggcgtgt tcgttgtagc tgaggatgca ggtgtattgg
     1861 acggagtgca ggtggacttt gaagatgtct ttgcagtctt gcacggtggc ggggcgtaac
     1921 agtgtcagca ggttcatggc agtatgtcgg cggcttcaga cggcatctgt gccgttggtc
     1981 ggattatagg gactgatgca gtttttttgc ttcttgaaat gcggtgtccg aatcggtggt
     2041 taaaacggta aagtgtccca ttttccgccc tttgtgcgcg gtttttttgc cgtaaaggtg
     2101 caggcgcgcg tccggccggc tttgcagggg aaaccaatcc ggtttgccgc cgtcttcctg
     2161 ccaaacgtcg cccaaaatat tcgccataca gcaagaactc agcaatttgg tgtcagcagg
     2221 tggcaggttg cacatcaggc ggacctgttg ctggaattgg tctgccgcgc aggcatcgat
     2281 ggtatggtgg ccggagttgt gcggacgcgg cgcgatttcg ttgacgacca attcatgcgt
     2341 gtcgccgaca acaaacattt ctaccgccaa tacgccgacg tagttcaatt catcggccaa
     2401 acgctgcgcc atttgtcgcg cctgttgctg aatgtcggca ctcagtcggg ctgggacgat
     2461 ggagtaggcg aggataccgt tttcgtgaat gttttcggca ggatcgaaag tttgcacgtt
     2521 gtcattgttc agacggcata cgataacgga aatttcgccg cgcaagtcca ccattttttc
     2581 caaaacgcaa tccacgccgc ggtgttcggc aaacgcggct ttgagttcat ccaccgtttt
     2641 gacgcggatt tggcctttgc cgtcatagcc caatgtagcg gttttcagga tgccgggcag
     2701 aaattgtatg ctttcttcag tgatgtcttc ggctttgcaa attgcttgat acggcgcggt
     2761 ttgcaggcct gctttgcgta tccatgactt ttcctgaatg cggttttgcg cgatggcaac
     2821 gcagtcgccg ctgggggaaa cattggtgtg tttggcgaga aaacgcatcg catcggcatt
     2881 gacgttttca aattcagtgg tcaccgccgc gcattttgcc aattcgtcca aagcagcttg
     2941 gtcgttaaac ggcgcgcata aatgacagtc ggcaaattct gctgccggcg cgtctggatc
     3001 ggggtcgaga acggtgactt tgtagcccat ggttttggcg gcaacggtaa acattctgcc
     3061 taattgtccg ccgccaagaa tgccgagcat ggcgggcgga gaaagagata tgtttttcat
     3121 gctgactctt caaattgtac aagttgatag ctataactaa ttcttgacgg atgtcttgta
     3181 tcgctggaat taccagtttc agaaatacag aatacttttt ccataaattt ttctgctttt
     3241 agaaattcca gtattctgtt tttttcatcc ttataagcac cgcggtctgt accccatgca
     3301 agaataatca tatcggcatc tttcaaacat cctttaaatt tggaaaaatc ggtttgggtg
     3361 tttgccctaa ttcctgtttg atgtgtagag taactggaaa aaatatttaa cattttgaag
     3421 ttggtaaaac cgtacatatc caagaaacgt gcaagttggg taagggtttt gtcgcttctt
     3481 tcatcatttg ctttacttgg attaattcct atagcgacag ctgagaaatt tttaggattt
     3541 tctgtgttgc cgctccatct aaccgttagg atttctcgat ttttttcatt atctgtatag
     3601 agaccgtctt tggtagtcgg tctgagtgtt tggcgaagct cataaagttt ttcataagtc
     3661 atttatccaa cccttcctgt accatttgcg cggcgtgtat gacggcacgg gctttgttct
     3721 gcgtttcctg ccattctgat tcaggattgg aatcggcaac cacgcccgcg ccgctttgga
     3781 cgtatagtgt gttgtttttt actacggcgg tgcggatggc gattgccaaa tccatgtcgt
     3841 tgttgaaacc ccatacgccg acggcaccgc cgtagatgcc gcgtttgctc ggttcgactt
     3901 cttcgatgat ttccatggcg cggactttgg gtgcgccgga gagtgtgccg gcagggaagg
     3961 tagcggcgag gatgtccatg ttggtcatgc cgtctttcag acggccttcg acgttggaaa
     4021 cgatgtgcat tacatgggag tatttttcaa tcaccatttt gtcggtaact ttgacttcgc
     4081 cggttttact gatgcggccg acgtcgttgc gtcctaagtc aatcaacatg acgtgttcgg
     4141 cgatttcttt ggcatcgctt aacaaatctt gttcgttggc aaggtcttcg gcgggggttt
     4201 tgccgcgcag gcgcgtgccg gcgatggggc ggacgataac gtcgttgcgt tcgcgtcgga
     4261 cgaggatttc gggcgaggag ccgacgatgt ggaaatcgcc gaaatcgtag tagaagagat
     4321 aaggcgaagg gttgagcgtg cgcaaggcgc ggtagagggc gagcgagctg tcggtgaatt
     4381 ccatgctcat gcgctggctg gggacaacct gcatgcagtc gcctgcaaag atgtagtctt
     4441 tgattttgtt aacgcaggct ttgaacggct cttcgccgaa ttcgctgacg gcttcggtgt
     4501 gtttgctgcc gagcgaaagc gggatggcgc aactttggcg taactgtgta cggatgtctt
     4561 cgaggcgttc gcgtgcgcgt tcgtagccgt cgggctgcga cggatcggcg taaacaatca
     4621 ggtagatttt accgcttagg ttatccacta ctgcaagctc ttgcgagagc atcaggagga
     4681 tgtcgggcgt gccgagcggg tcggctttgg tggtgttttt caggcggtgg gcgaagtgtt
     4741 caaaattgta gatggtttcg taaccgaagt agccgaccag tccgccggta aagcgcggca
     4801 ggcttgggat ttcgggtgtt ttgaagcggt tgtggaaggc ttcgataaag ggcagggggt
     4861 tgccgtcgtg ttgctcgaca atttcgccgt tttgataaac atcgacgtgt ttgccgctgg
     4921 ctttgagata gtggctgcaa ggcaggccga taaaagaata gcggccgaaa cgttcgccac
     4981 cgacaacgga ttcgagcagg taggtatagg ggcggttggc gagtttgaga tagagggaaa
     5041 gcggcgtatc caaatcggca aggagttctt gcacgagcgg gatgcggttg tagccttgag
     5101 cggcttgggc ttggtattct tgtttgctga tcatttctgc tttcccaaag ggcggtttcg
     5161 gacggcgcgg caacgggcgc gagtatagca ttttatcgga attgttgaca gtctgaccgg
     5221 agatgccgtc tgaaaaggac gggcttacgg ctttgttttg cgctgccggt attgtttcca
     5281 tttaaacaga taacctgcgg ttacgggaat cagggctaag atggttttga acgtccatga
     5341 tgcataccaa ggtaaagaac cgggtgcgac cgattgattc agatacggcc accaacaggc
     5401 agtcaggcag ccgagcagca gcggccagat atgccggctg aggttttttt gccaaaggag
     5461 gaatgatgcg gcagaccaaa gcagggtaag ggcagtgatt tttaacacgg tataagcatc
     5521 cgctccaaac agggcggcga gaaaatacgt accggcaatc cagaggacgg caagtatcag
     5581 gacaatgatt tcttcaggtc gtttgaacat ttttttcttc ctgtttgatt tcagacggca
     5641 ttgccgttct gtttggtttc cagcagctcc cagcgttcca gcttttccaa aagcagcatt
     5701 tcgatttctt cggcgcggct ttgcagtgcg ccggcttttt cgtaatcttt gaagatttcg
     5761 ggatcggaaa gctgcgcgtt gatttctgcc tgttcggctt ccaaggcggc gatttcgtcg
     5821 ggcagggcgt cgagttcgcg ctgttctttg taggacagtt tgaccgtgcg gttggctttg
     5881 ggtttttctt tcaccggttc ggcatcggct gttttgggtg cggattccgt ctgaatcttc
     5941 gcttcgcgtg attttgcgtc gatgtagtcc tgatagccgc cgatatattc ttttaacctt
     6001 ccatttcctt caaaaataat gctttgggta atcacgttat cgaggaacat acggtcgtgt
     6061 gagacaagga ataccgtgcc ttgataatcg cgcagcaaat cttcaagcag ctcttgggta
     6121 tcgatatcca agtcgttggt cggttcgtcc aaaatcaaaa tattagcagg acgggtaaag
     6181 agttttgcca gcaaaaggcg gttgcgttcg ccgccggaga gcgatgaaac gggactttgt
     6241 gcgcgggctg gatggaataa gaaatcttcc agatagctca ttacgtgttt tttcttgccg
     6301 ccgacttcga cgtaatcgtt gccttgaccg agcgtgtaaa acacggtgtc gttttcgttc
     6361 aacgtgctgc ggaattggtc gaaataggca acttcctgct tactgccgat acggattctg
     6421 ccgtaagtcg ggttgagttc gcctaaaatc agttttaaaa atgtggtttt gccgatgccg
     6481 ttggggccga ttagaccgat tttatcgccg cgctgcaaaa tcgcggagaa tttgtccata
     6541 atgactttgt cgccataggc aaacgaggca tgttccaatt cggcgatgat tttgccgctt
     6601 ttctcgccgc tgtcgagctt gaagttgacc tgtccctgca cattgcggcg ttcggcgcgt
     6661 tggcggcgca gctcttccaa acggcgcacg cggccttcgt tgcgggtgcg gcgcgctttg
     6721 atgcctttgc gtatccacgc ttcttcctgc gcgtggaatt tgtcaaacag gcggttgtgt
     6781 tccgcttcaa ccgccaattc ctgcactttt ttttcgctgt atttggagaa cgagccggga
     6841 taggaacgca aaataccgcg atcgagttcg acaatccgcg tggcgatatt gtccaaaaaa
     6901 cggcggtcgt gggtaatcac aaccaagctg ccttcaaacg ctttgagcag attttccagc
     6961 caaataatcg cgtcgatatc caaatggttg gtcggttcgt ccagcagcaa tacgtcgggc
     7021 ttctgcaccc aagcctgcgc caaggcgaca cgctttttct gaccgccgga aaggttgccg
     7081 attttttcgt tttccggcaa accgagttcc cccaaagtct gcttgactgc cgcatccagc
     7141 ttccagccgt ccttcgcttc gatttcaagt tgtaattcgt tgagttcttt caacaaagcc
     7201 tcacccgaac cgttttccaa ctcatggccg acgcggtggt aacggcgcaa taaatcgcga
     7261 atttcaccca aaccttcggc aacggtatca aatacggttg cgtccttatc aaaaaaggat
     7321 tcctgcggta cataaacgat tttgaggttg ttttgaatga taatctgccc gtcgtcgagc
     7381 ttttgcacgc cggcgaggat tttcagaaac gaagacttac ctgcgccgtt gcgtccgatc
     7441 aagccgactt tttcgccgct gtcgagttga aaagaagttt tgtcgagcaa ggcaacgtgg
     7501 ccgatggcaa aagaagcgtt ttctacagat aatatattca tgatgcaaat tctcaacagt
     7561 taccgtttgg attttaccgc aagtttggcg cgggcaattt caaccgcacc cggcaggacg
     7621 gaaacaataa tgatgccgcc catcaccaag cccagattgt tttttacgac ggggaagttg
     7681 gcaaagaaat agcccgcgta agaaaacagg ataacccaca acaagccacc gatgatgttg
     7741 tagcggataa atttggcata gtgcattttc cccataccgg cgacaaaggg ggcgaaggtg
     7801 cggacgatgg gtacaaaacg ggcaatgatg atggttttcc cgccgtgttt ttcgtaaaaa
     7861 cggtgggttt tatcgagata ttcgcgtcgg aagattttag aatcgggatt ggcgaacagc
     7921 ctgccgccga aatatttgcc gacggtaaaa ttgagcgcgt cgccgagtat ggcggcaagg
     7981 cttaataatg caaccatcaa atgaatatcc ataccgccca gcgcggcaat cccgccggcg
     8041 gcaaacagca gcgaatcgcc gggcagtaag ggcgtaacaa tcaggccggt ttcgcaaaaa
     8101 acaatcaaaa acagaatcgc ataaatccac acaccgtatt gcgccgacag cgcaagcagg
     8161 tgttggtcga tatggaggat gaagtcgatg gcggaggcaa gcacggcgcg ttcctaaaaa
     8221 aaacaaaccg cgtattttaa ccgattggaa aaatgccgtc tgaaattttc agacggcatc
     8281 ggctattcaa attcatttca cgtaaaaacc gcaaaccaaa atagtttgcg gtttggcatt
     8341 taaagtgaca atgatgattt caaatcatca gaattttatg ccgacgcgca agccgtattc
     8401 acgaatactg gttttcggga tggtgagcga tacgtcgcca ctcttggttg ttacactaaa
     8461 ctcgccggat tctttgtaag tgcgttgttt gtagaacggc cccgcctcga tgctggcgga
     8521 ttcgcccagt tttttaccaa tatttgcacc caatccaaag ccccagccgt tggttttttg
     8581 attgatgtct tttttgagat tggtaacgcc ggtgtttaat ttatagcggg aattgaggtc
     8641 aaatttcact tcagaccaag ggttgatata ccagccgtta cccagttggg aaagcaaatc
     8701 cgcgtgaact ttggctaacc acgactgacg gctgctgtga agcgtatgct tggtggtttt
     8761 aatgctgtct tttgaagatt caaaacccaa gccggcaccc acacggaaat ttaaagaatc
     8821 acttaacgtt tgggtgtagg tgtagcctgt gtaaagatcg atacggtttt caggaacgcc
     8881 ggtgggcagt tttacatttt tagtcttacc cagcttgttc tcatctgttt ccaaattaat
     8941 aatatttttt ttgctgcgcc cgaaaccggc ttccaagcgg atgccttggt tggcatcaaa
     9001 aggaatatca gcacgtacgc cgatgtgttt ggcagctttg tgtttttctt tcaggaaagc
     9061 acgagttgaa gaaatggaag agaggtcggt gtggacggta aactcattag cggtttgaag
     9121 ctcttgtgca gcggcggcag taccggtcag ggcaatcatg gcacatgtaa aaactgtttt
     9181 tttcatagtt aaaatctcca aaatttggat tgtagtcgga tatggtaaca taacgtaaat
     9241 aatcgttacg cttacaatta tattcttaag ctttcggggg ggggggggga ttttacatat
     9301 attaataaaa attaacaaat agttatttgt ttacaacgaa ttgttattct cacttggttt
     9361 tctgtttttt atgggaatga cga
//
LOCUS       DAWKQJ010000068         9322 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000068 DAWKQJ010000000
VERSION     DAWKQJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.068"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..101
                     /locus_tag="WJL20_001203"
     CDS             <1..101
                     /locus_tag="WJL20_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEZ7683360.1"
                     /translation="TFACVLGTLVASEHGATPDISPERIERFMNPV"
     gene            complement(161..718)
                     /locus_tag="WJL20_001204"
     CDS             complement(161..718)
                     /locus_tag="WJL20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7683361.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WJL20_001205"
     CDS             complement(794..2428)
                     /locus_tag="WJL20_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ7683362.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2830..3936)
                     /gene="serC"
                     /locus_tag="WJL20_001206"
     CDS             complement(2830..3936)
                     /gene="serC"
                     /locus_tag="WJL20_001206"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ7683363.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4301..4732
                     /gene="rimP"
                     /locus_tag="WJL20_001207"
     CDS             4301..4732
                     /gene="rimP"
                     /locus_tag="WJL20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ7683364.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4760..6262
                     /gene="nusA"
                     /locus_tag="WJL20_001208"
     CDS             4760..6262
                     /gene="nusA"
                     /locus_tag="WJL20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ7683365.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6274..9162
                     /gene="infB"
                     /locus_tag="WJL20_001209"
     CDS             6274..9162
                     /gene="infB"
                     /locus_tag="WJL20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ7683366.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFINVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQLTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2491 a         2692 c         2364 g         1775 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtacgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttcccccga acgcatcgag cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg catttcgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt acaccaatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aagtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccgac cgggcggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggcg gctgtgcggg ggttccgcct gccattcaaa cgtccggcag
     2581 acataaaaac atcgtaagca agattcgaac cggtctgcaa ccgcccctgc cagaaaaacg
     2641 ggcaaagcat ttcatattgg aaaaacccga ccgcgccgcc gacgggacag tccggcacaa
     2701 acagcatcac gctcggattg aaaaggacgg atagccgccg gcagcacaat cttaccacct
     2761 ccaaaacgcc gccggagaac gccgaaacag ccgatgccgt ctgaagccgc ttcagacaac
     2821 atcgggacat caaccgtaac gccgttggaa atcgcgcata aaatctgcca aagcccgcac
     2881 gccttcaagc ggcatcgcat tataaatgct ggcgcgcatc ccaccgacag acttatagcc
     2941 cttcaacagg cacaagccct gcaattcggc ttccagcaca aaacggcggt caagctcctc
     3001 atcccccgtt tggaacacga cattcatttt agaacgcgca ttcggacgga tacggttgat
     3061 atagaaaccg ccgctgccat ctatcgtctc atacaaggtt tgcgccttca gccgattgac
     3121 cgcttcaatt tttttcacac cgccctgcgc ctgcagccag cggaacacca gccccgacat
     3181 ataaatcgcg taagttgacg gcgtgttgta cataccttcg cggttcaaat gcgaacggta
     3241 gttgaacaca tcgggaatat cgttcggaca acgctcgagc aaatcctcac gcacaatcac
     3301 caccgtaacc cctgccgggc cgatgttttt ctgtgcgcct gcgtaaatca gtccgtagtc
     3361 ggcaacatca aactcgcgcg acaaaatctc gctggacata tcgcacacca gcggcggcat
     3421 gccttctgaa aggcgcggca cttcacggta ttgcagcccg ttgaccgttt cattgacggc
     3481 aaaatggaca aacgccgaat cgggtgcaac atcccacgtt tccacaggcg gcaggtcgag
     3541 atagtcgaac tgctcgccgc catgcgccgc caaacggatt tccgtatcgg tcaaacggct
     3601 catctgttca taagcgatac ggctccagtt gcccgttacc accgcgtcgg cagtgcggaa
     3661 accgtgtgcc agattcatgg ctgccatatt aaattgggtt gttgctccgc cctgcagaaa
     3721 caatatctta tagttgtcag gcactttcaa aagctgcctc aaatcctgtt ccgcatgatg
     3781 caggatgctc aaaaacattt ccgaacggtg gctcatcgcc atcacgggaa aacccgtacc
     3841 gttgtagtcc aacatttcct gccgcgccgt ttccaacacg gcttcgggca atacggcagg
     3901 gccggcggaa aaattgtaaa tcggataaag agacatgatg cagccttgat tctgaacaat
     3961 aacccgcccg attttaggct tgcagcaggc tttgtgcaag gatggaaaat acctgtcctc
     4021 cgccccgctt catgccgccc gaacacagaa aacaacatca atatattgat ttacaaacat
     4081 aaaaatcatg cacgcgacaa atagatacat ttgttttgtc aacaatattc acgatttccc
     4141 attacaaacc tcccttacat ccgctttttt ccgtcccaaa aacacaaaat aaatcaacac
     4201 tttcatttct ccgcaaaagc ggttataatt acgccgattt ttcaactttg acgaaaaatg
     4261 gccacggggc cattttttta ttatgtacat agggagcagc atggatatcc aaaccatcct
     4321 cgaaaaaacc ctgcccggcc tgggctacga actggtcgat ttcgaactga ccgcgcaagg
     4381 aacattgcgc gtgttcatcg acaaagaaag cggcattacc gtcgaagact gcgcaaccgt
     4441 cagcaaccac ttgagccgcg tcttcatggt tgaagacatc gactacaaaa acctggaaat
     4501 ttccagcccc ggactcgacc gccccttgaa aaaagccgcc gacttcgtgc gctttgccgg
     4561 tcagaatgcc aaaatcaaaa cccgcctgcc gatagacggt cagaaaaact ttatcggtaa
     4621 aatcgaaggc tgcgaaaacg ataccgttac cgtatccttc gacggcaaaa ccgtacaaat
     4681 cgaattgggc aacatcgaca aagcccgtct gcgccccgaa ttcaaattct aaaacacaac
     4741 aatattggag atgttcaaaa tgagtcgtga aatgttacag ctggcagaag cactggcaag
     4801 cgaaaaaaac gttgatgcgg aagtcgtctt ccaagcactg gaattcgccc tgtctaccgc
     4861 cgccaagaaa aaggcagacc gcgaacacat ggacgtgcgc gtccaaatca accgcgacac
     4921 cggcgaatac caaaccttcc gccgctggct gattgtcgcc gatgaagact atacctatcc
     4981 cgatgtcgaa aaaaccatcg aggaaatcca agaggaaatt cccggcacta ccatccaaat
     5041 cggcgaatac tacgaagagc agctgcccaa cgaaggcttc ggccgccaag ccgcgcaaac
     5101 cgccaaacaa atcatcctgc aacgcatccg cgatgccgag cgcgagcaga atctgaacga
     5161 gtttctcgcc gtcaaagaag acatcgtgtc tggcacggtc aaacgcgtcg aacgccacgg
     5221 catcatcgtc gaagtcgttg ccggcaaact ggacgcgctg attccgcgcg accaaatgat
     5281 tccgcgcgaa aacttccgca gcggcgaccg catccgcgcc ctcttcctgc gcgtcgaaga
     5341 aatcggcaac accggccgca aacaagtcat cctgagccgt acttccggcg atttcctcgt
     5401 caaactgtac gccaatgaag tacctgaaat tgcagacggc atgcttgaaa tccgcgctgt
     5461 cgcccgcgac ccgggccagc gtgccaaagt cgccgtcaaa gccaacgacc agcgcatcga
     5521 tccgcaaggc acctgtatcg gcgttcgcgg ttcgcgtgtc aatgccgtca gcaacgaatt
     5581 gtccggcgag cgcatcgacg ttgtcctttg gtcgcccgag cctgcccaat tcgtcatgag
     5641 cgcgctctca cccgccgaag tcagccgcat cgtcatcgac gaagacaaac acgccgtcga
     5701 tgtcatcgtt gccgaagacc agctcgcgct cgccatcgga cgcggcggtc aaaacgtgcg
     5761 ccttgcttcc gacctgactg gctggcagct caacatcatg acttccgccg aggcagacga
     5821 acgcaatgcg gcagaagatg ccgccatccg ccgcctgttt atggatcact tgaacgtgga
     5881 cgaagaaacc gccgacgtac tggttcagga aggttttgca accttggaag aagtcgccta
     5941 tgttcctgcc gccgaactgc ttgccattga aggatttgac gaagaaatcg tcgatatgct
     6001 ccgcaaccgc gcccgcgatg ccatcctgac catggcgatt gccgccgaag aaaaactggg
     6061 cgaagtatcc gacgatatgc gcaacctcga aggcatagat gccgatatgc tccgcagcct
     6121 tgccgaagca ggcattacca cccgcgacga cttggcagag cttgctgtgg acgaactgat
     6181 tgaaatcacc ggtgtaaacg aagaaaccgc aaaagccgtc atcctgaccg cacgcgaaca
     6241 ctggtttacc gaagacaaat aaagggggta cagatgagta acacaaccgt agaacaattt
     6301 gccgccgagc tgaaacgccc cgtcgaagac ctgttgaaac agttgaaaga agccggcgtc
     6361 agcaaaaaca gcggcagcga ttccctgacg ctggacgaca aacagcttct gaacgcctac
     6421 ctgaccaaga aaaacggcag caacggcggc accatcagca tccgccgcac caaaaccgaa
     6481 gtcagcaccg ttgacggcgt aaaagtcgaa acacgcaaac gcggacgcac tgtcaacatt
     6541 ccttctgccg aagaattggc agcacaggta aaagccgccc aaacccaagc cgcacctgtc
     6601 cggccggagc agacggcaga agacgcggca aaagcccgag ccgaagctgc cacacgtgca
     6661 gaagcccgtg ccaaggcaga agcggaagcg gcaaaactga aagcggcaaa agcaggcaac
     6721 aaagccaaac ctgccgcgca gaaacccacc gaagcaaaag ccgaaaccgc acccgttgcg
     6781 gcggaaacca aacccgccga agaaagcaaa gcgggaaaag cccaagccga caaaatgccg
     6841 tctaaaaaac ccgccgagcc caaagaaaaa gccgccaagc cgaaacacga gcgaaacggc
     6901 aaaggcaaag atgccaaaaa accggcgaaa cctgccgcac ctgccgtgcc gcaacccgtg
     6961 gtcagcgcgg aagaacaggc gcaacgcgat gaagaagcac gccgtgccgc cgcacttcgc
     7021 gcccaccagg aagccctgtt gaaagagaaa caggaacgcc aggcacgccg cgaagccatg
     7081 aaacaacagg cagaacaaca ggcaaaagcc gcacaggaag ccaaaaccgg cagacagcgt
     7141 cccgccaaac ctgccgagaa accgcaggca gccgcgccgg ccgtcgaaaa caaacctgtc
     7201 aatccggcaa aagcgaaaaa agaagaccgc cgcaaccgcg atgacgaagg tcaaggccgc
     7261 aacgccaaag gcaaaggcgg aaaaggcgga cgcgaccgca acaatgcacg caatggcgac
     7321 gacgagcgcg tacgcggcgg caaaaaaggc aaaaaactca aactcgagcc gaaccaacac
     7381 gccttccaag caccgaccga acctgtcgta cacgaagttt tggtgcctga aaccattacc
     7441 gttgccgact tggcacacaa aatggcagtc aaaggcgtgg aagtggtcaa agccctgatg
     7501 aagatgggca tgatggttac catcaaccaa tccatcgacc aagacaccgc cctgattgtc
     7561 gtcgaagaac tcggacatat cggtaaacct gccgcagcgg acgaccccga agccttcttg
     7621 gacgagggcg cggaagcagt ggaagccgaa gcattgccgc gtccgcccgt cgttaccgtg
     7681 atgggccacg tcgaccacgg caaaacctcg ctgctggact acatccgccg taccaaagtg
     7741 gtacagggcg aagcgggcgg cattacgcag cacatcggcg cgtaccacgt tgaaacccct
     7801 cgcggcgtga ttaccttctt ggacaccccg ggccacgaag cctttaccgc tatgcgcgca
     7861 cgcggtgcga aagcaaccga catcgtgatt cttgtggtcg ccgccgacga cggcgtgatg
     7921 ccgcaaacca tcgaagcgat tgcccacgcc aaagcggcag gcgtaccgat ggtggttgcc
     7981 gtcaacaaaa tcgataaaga agccgccaac ccagagcgta tccgccaaga gctgaccgca
     8041 cacgaagtcg tgcctgacga atggggcggc gatgtacagt ttatcaacgt ttccgctaaa
     8101 aaaggcctga acatcgatgc attgctcgag gcagtcttgc tcgaagccga agttttggaa
     8161 ctgaccgcac ctgtcgatgc gcccgccaaa ggcatcatcg tcgaggcgcg cttggacaaa
     8221 ggccgcggcg cggttgccac attgctggtt caaagcggta cgctgaaaaa aggcgacatg
     8281 ctgctggccg gtacggcatt cggcaaaatc cgcgcgatgg tcgatgaaaa cggcaaatcc
     8341 attaccgaag ccggcccgtc catcccggtc gaaatcctcg gcttgtccga cgtacccaat
     8401 gcgggcgaag acgcgatggt attggcggac gagaaaaaag cgcgcgaaat cgccctcttc
     8461 cgccaaggca aataccgcga cgtgcgcctt gccaaacagc aggcggcgaa gctggaaaat
     8521 atgttcaaca atatgggcga aacccaggcc caatctttgt cggtcatcat caaggcagac
     8581 gttcagggct cttacgaggc tttggcgggc agcctgaaaa aactgtccac cgacgaggtg
     8641 aaagtgaacg tgttgcacag cggcgtgggc ggcattaccg aatcggatgt caaccttgcc
     8701 atcgcttcgg gcgcgttcat tatcggcttt aacgtgcgtg cagatgcctc ttcgcgcaaa
     8761 cttgccgaaa atgaaaacgt ggaaatccgc tactacaaca tcatctacga tgccatcgac
     8821 gacgtgaaag cggcgatgag cggtatgctt tctcccgaag agaaagaaca gcttaccggc
     8881 acggtcgaaa tccgtcaggt catcagcgtt tccaaagtcg gcaacattgc aggctgtatg
     8941 gttaccgacg gcgtggtcaa acgcgattcc catatccgcc tcatccgcaa caacgtggtc
     9001 atccataccg gcgagctgtc gtctttgaaa cgctacaaag acgatgttaa agaagtccgt
     9061 atgggcttcg agtgcggcct gatgattaaa ggcttcaatg aaattatgga aggcgaccaa
     9121 ctggaatgct tcgacatcgt cgaagttgca cgcaccctgt gattttgatg cggaaaaatg
     9181 ccgtctgaag cgttcagacg gcatacgaaa cgggttctgt atcatacaga acccgttttt
     9241 tgtcgcaaat cggcttcaga cagccctctt gccttatccc gatttgaatc tgacttgcca
     9301 tacaaacagg cttcagacgg ca
//
LOCUS       DAWKQJ010000069         9299 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000069 DAWKQJ010000000
VERSION     DAWKQJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.069"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(91..1371)
                     /locus_tag="WJL20_001210"
     CDS             complement(91..1371)
                     /locus_tag="WJL20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ7683367.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WJL20_001211"
     CDS             complement(1593..2213)
                     /locus_tag="WJL20_001211"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ7683368.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMVRIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WJL20_001212"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WJL20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ7683369.1"
                     /translation="MLRKLLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEVDAGSSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WJL20_001213"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WJL20_001213"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ7683370.1"
                     /translation="MDNPATAPWQDGWLQSVRHIPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="WJL20_001214"
     CDS             4118..5404
                     /locus_tag="WJL20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ7683371.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..8077
                     /gene="ligA"
                     /locus_tag="WJL20_001215"
     CDS             5552..8077
                     /gene="ligA"
                     /locus_tag="WJL20_001215"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ7683372.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8110..>9299
                     /locus_tag="WJL20_001216"
     CDS             8110..>9299
                     /locus_tag="WJL20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ7683373.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQ"
BASE COUNT         2197 a         2801 c         2335 g         1966 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tgacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttaccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttctatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcaccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcaaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggctcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggattttc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tctgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcggacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtgcc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg caatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcagcc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccccgtt tccttttcga
     2641 taaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcgttcaga cggcgttgca tcgccttgta ggcggtttgg taaatctgca
     2761 aatcgctgct acccgcgtca acttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgtccgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc aatctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aattcggtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagtccattt caacaatttt ctcaacatgg taggattccc aatatcggaa tacatcttaa
     3301 aattcgggcg acagtataac ccaaattacc ccataagaca gaaaggacat catggacaat
     3361 cccgctacgg caccctggca ggacggttgg ctgcaaagcg tgcgccacat tccgtcgccg
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatacgg cacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 ttgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcgggc actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggtc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaactcc aagaccctgc tatgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaaccc gttcccgttc cgcaaacgcc
     4561 ggcaaaaccg ctgattacgc tcaaagagct gtcaaaagtc gaactgccgt ggtttgacgt
     4621 gcgcttcgat ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 cctggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa tgcccttttg gacaaccaat cctataaagg
     5161 cttcagtatg ctgctcgata ttccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcaaccc aatggctcaa agacgtacgc acttatgtat tggctcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ccgccacctc accgacctgc tcaaccgcta tgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt attccgcgag ctcgaagcgt tggaattaaa
     5761 ccatcccgag ctcaaactgc ccgacagtcc gacccagcgc gtcggcggcg agcctttagc
     5821 gggatttgcc gaagtgcgcc acgaagtgcc gatgctgtcg ctgaccaacg ctttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac gctttcgacc aacgcgtgcg
     5941 cgacggtttg gacggcggca aacccgaata cgttatcgaa cccaaattcg acggcctcgc
     6001 catcagcctg ctctaccgcg acggcgtatt ggtacaggcg gcaacgcgcg gcgacggcac
     6061 aacgggcgaa gacgttaccc aaaacatcaa aaccgtcgcc aacatccccc tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgct gccggcagcc tgcgccaact cgattcgcgc atcactgcgc aacgcaaact
     6301 gcactttttc ccctactccg tcgcccgcca gcaaggcggt ttcgtcgcgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg ccaaatggca atttcggctg
     6421 tttcaaaaat atcgacgaag tattggcgtt ttacgaacaa atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gtatggtggt caaagtcaac agcttggcgc aacagcgcga
     6541 actcggcttc atctcacgcg cgccacgctg ggcgattgcc cacaaattcc ctgccgaaga
     6601 agccctgacc attgtcgaag cgattgacgt gcaaatcggc aggacgggcg cggtaacgcc
     6661 ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc gttaccaacg ccaccctgca
     6721 caatcaggac gaagtttccc gaaaagacgt gcgcgtcggc gataccgtcg tcgtgcgccg
     6781 cgctggagac gtgattcccg aagtcgtgcg tgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgccgtt gctgtttcag acggcatcgg gcatcggcag gacgatttgt ttgccgaaac
     6901 accgtccgct aatcaaaccc aatccgttcc gctccacaag ccctaccgcc tgccgaccca
     6961 ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggc gaagccgtcg cccgatgcag
     7021 cggcggtatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agccatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaaaga
     7201 aacgacggac aaaaccgttg ccgaatcgga tcaaacgccg tctgaaggca gttcggtcgg
     7261 cgcgtcgggc aaacataaaa aacaaccggt caagtgggcg gaaaacatcc tcgggggcat
     7321 agaatccggc aaaacgcccg aactcgcgcg cttcctgttc gcgctcggca tccgccacgt
     7381 cggcgaacgc accgccaaaa cgctggcgca ggcattcggc acattggaac gcgtccgccg
     7441 cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt acggtagtgg cgcattccat
     7501 tgcccacttc ttcgcccaag ccgaacagca ggcgatgata gacgaactgc tcgccgcagg
     7561 cgttgccccg caaacccaag ccgttaccat cccgcccgcc cgccacgccg aaccgcaacg
     7621 ctggatcgcc cgcctgcccg acttcaaaat tagcgaaaac aaagcccaag ccttatggga
     7681 actcgctggt aaaaacatag aaggtctaca aaacgacaaa gccctccccg ccgactggca
     7741 agcatggcgc agcgaaccac aaaacgccgc cctactcgaa aacctgaaaa ccttcttcgc
     7801 gcaaatgccg tctgaagacg aggcggcgca aggttcagac ggcatcaata aagccgtagc
     7861 aggcaaaacc tttgtattaa ccggcaccct gcccacgctc aaacgcgacc aagcccaatc
     7921 cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtg tccaaaaaaa ccgactacgt
     7981 cgtcgccgga gaagccgcgg gcagcaagct ggaaaaagcc aatgccttgg gcgtttccgt
     8041 cctcagcgaa gccgaactgc ttaccctgct cggctgaacc tatgccgtct gaaccgcctt
     8101 cagacggcat tgtccacttt tccgaaccac accatgcaca ccatttcctt tccaaaccgc
     8161 acccgactca ccgccctgcc gcccctgtcg ctttacatcc acatcccgtg gtgcatcaaa
     8221 aaatgcccgt attgcgactt taattcccac agcctgaaaa acggattgcc cgaagccgcc
     8281 tatatcgacg cgctgctgac cgacttgcag ctcgaattgc ccaatatttg gggcaggccg
     8341 gtggaaacga tctttttcgg cggcggtacg cccagcctgt ttcaggcgga atcgattgac
     8401 cgtttattga gcggcgtgcg ttcgctgttg cgcttgcagc cggaagcgga aattaccttg
     8461 gaagccaatc cggggacatt tgaaatcgag aagtttcaag gatttaaaga cgcaggcatc
     8521 acgcggcttt ctattggtgt acagagtttc aacgacgata tgctctcaag gctggggcgc
     8581 gtccacaacg gcaaggaagc cttaaccgct atcgataccg ccttgaaatt atttgataaa
     8641 gtcaatatcg acttgatgta tgccctgccg aaccaaaccg ttcagacggc attggacgac
     8701 gtacaaaccg ctatcgctac gggcgcgacc cacatcagcg cgtatcatct gacgatggaa
     8761 ccgaacacgc cgttcggtca tacgccgccg aaaggtttgc cgcaagacga agccgccctc
     8821 gacatcgaag acgcggtaca cggcgcgctg gaaagcgcgg gttttgtcca ctacgaaaca
     8881 tcggcttttg cgaaaccagc catgcagtgc cgccacaatt tgaactactg gcagttcggc
     8941 gattatttag gcataggcgc gggcgcgcac ggcaaaattt cctatcccga ccgcatcgag
     9001 cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct taatgcaaaa ccgaccgagc
     9061 gaagccgtcg aacgcaaaac cgtcgccgcc gaagatttgc cgtttgagtt catgatgaac
     9121 gccctgcgcc tgaccgacgg cgtacccgcc gcgatgttgc aggaacgcac gggcgtaccg
     9181 agtgccaaaa tcatggcgca aatcgaaaca gcaaggcaaa aaggcctgct ggaaaccgac
     9241 cccgccgtat tccgcccgac cgaaaaagga cgcttgtttt taaacgattt gctgcaatg
//
LOCUS       DAWKQJ010000070         9281 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000070 DAWKQJ010000000
VERSION     DAWKQJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.070"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..142)
                     /locus_tag="WJL20_001217"
     CDS             complement(<1..142)
                     /locus_tag="WJL20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEZ7683374.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGY"
     gene            381..1280
                     /gene="crgA"
                     /locus_tag="WJL20_001218"
     CDS             381..1280
                     /gene="crgA"
                     /locus_tag="WJL20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ7683375.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAIP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSCFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1507..4722)
                     /gene="carB"
                     /locus_tag="WJL20_001219"
     CDS             complement(1507..4722)
                     /gene="carB"
                     /locus_tag="WJL20_001219"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ7683376.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGVLSDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLSPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4729..5277)
                     /locus_tag="WJL20_001220"
     CDS             complement(4729..5277)
                     /locus_tag="WJL20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683377.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5280..5843)
                     /locus_tag="WJL20_001221"
     CDS             complement(5280..5843)
                     /locus_tag="WJL20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7683378.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6695..7084)
                     /locus_tag="WJL20_001222"
     CDS             complement(6695..7084)
                     /locus_tag="WJL20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ7683379.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7828..8961)
                     /gene="carA"
                     /locus_tag="WJL20_001223"
     CDS             complement(7828..8961)
                     /gene="carA"
                     /locus_tag="WJL20_001223"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ7683380.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2104 a         2340 c         2396 g         2441 t
ORIGIN      
        1 catagccggc atcaatcacg gtttcttgaa cattgtgtcc gagcgcggtc aaaacttctt
       61 tcgctttttt gtgaagcgtg cggtttaacc cgccgtgaga atgtccgaac gctttgccgc
      121 cgtctaataa taaaatattc atttgttacc tcgtttgtga attgatgaat gtattgtgcc
      181 gttttacttt ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata
      241 atcagatgtt ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt
      301 cggaaggggt tacacccctc ccaaataaag tctgatcctg ccgccctaaa gggcggggtt
      361 ccaaccgaaa aggaaacacg atgaaaacca attcagaaga actgaccgta tttgttcaag
      421 tggtggaaag cggcagcttc agccgtgcgg cggagcagtt ggcgatggca aattctgccg
      481 taagccgcat cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc aaccgcacca
      541 cgcggcaact cagtctgacg gaagaaggcg cgcaatattt ccgccgcgcg cagagaatcc
      601 tgcaagaaat ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg
      661 tgttgcgcgt ggattccgcg ataccgatgg tgctgcatct gctggcgccg ctggcagcaa
      721 aattcaacga acgctatccg catatccgac tttcgctcgt ttcttccgaa ggctatatca
      781 atctgattga acgcaaagtc gatattgcct tacgggccgg agaattggac gattccgggc
      841 tgcgtgcacg ccatctgttt gacagctgct tccgcgtaat cgccagtcct gaatacctgg
      901 caaaacacgg cacgccgcaa tctacagaag agcttgccgg ccaccaatgt ttaggcttca
      961 ccgaacccgg ttctctaaat acatgggcgg ttttagatgc gcagggaaat ccctataaga
     1021 tttcaccgca ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt
     1081 gcggtattgc ttgcttatca gattttttgg ttgacaacga catcgctgaa ggaaagttaa
     1141 ttcccctgct cgccgaacaa acctccgata aaacacaccc ctttaatgct gtttattaca
     1201 gcgataaagc cgtcaatctc cgcttacgcg tatttttgga ttttttagtg gaggaactgg
     1261 gaaacaatct ctgtggataa ttctataaaa acctgtcata aaactgtttt tactcactta
     1321 ataactttga gaaaaaataa ataagatgat gtatagccgg cggcaatggc agtctatatg
     1381 gggagcgtga tagggctgtt ccttatgtac ggcttgtgtg tggcaacatc ggctttccaa
     1441 atgacaaaat gccgtctgac accgtaaaaa aaggtttcag acggcatttt caacctgatt
     1501 caggcatcag ttgcggtttt tcaaacgccc gtgcaactct tgaacgctgt acacgcccag
     1561 atggtctcgg cttttcgcgc cttcgctcat cgcttcgccg ccggcggtgg tggtgtattg
     1621 cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg ctgtcggaca cggattgcgg
     1681 atcgctggaa acggtgttca cgaccagtgc gatttcgccg tttttcagcg cgtcgccgat
     1741 gtgcgggcgg ccttcgggga ctttgttgat ggtctgcaca atcagcccgt gttcggtcag
     1801 gtattgcgcc gtgccgcgcg tggcgcagat gccgtagcct aaggcttgga agtttttagc
     1861 ggttttgatg acgcgttctt tgtcttcttc gcgcacggag aggaagattt tgccggtcgg
     1921 gctgaggcgt tcgcccgcgc cgagttgggc tttgtagtag gcttcgccga aacttgcgcc
     1981 cacgcccatc acttcgccgg tggagcgcat ttccggtccc aaaatcgtat ccacgcccgg
     2041 gaatttgatg aatgggaaca cggcttcttt aacggcatag aaatcgggga caacttcttt
     2101 ttccacgcct tgttctttca gggaaatgcc tgccatacag cgtgcgccga ctttggcgag
     2161 cggcacgccg gtggctttgg agacgaaggg cacggtacgg ctggcgcgcg ggttcacttc
     2221 caacacgaac accacgccgt cttgtacggc aaactgcacg ttcatcagtc cgaccacgcc
     2281 cagcgcgtac gccatggctt tggtttggcg gcggatttcg tcttggattt cttcgctgag
     2341 cgagtagggc ggcagcgagc agccggagtc gccggagtgg atgcccgcct gttcgacgtg
     2401 ctgcatgatg ccgccgataa ccacatcttt gccgtctgaa acgcagtcca catcgacttc
     2461 aatcgcgttg ttgaggaaga aatcgagcaa cacggggctg tcttcggaaa cttgtacggc
     2521 ttcgcgcata tatttttgca actgctcggc agagtgaaca atctgcatcg cgcgtccgcc
     2581 caaaacataa gacgggcgca cgaccagcgg atagccgatt tcttcggctt tgacgagtgc
     2641 ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc agacctaagt cgttcaacac
     2701 tttttggaag cgttcgcggt cttcggcggc gtcgatgctg tcggcggacg tgccgatgat
     2761 gtttacgccg ttttcaacca acgcgttggc gagtttcagc ggggtttgac cgccgtaatg
     2821 cacaatcacg ccccacgggt tttcggtgcg gacgatttcc aacacgtctt ccaatgtcag
     2881 cggctcgaaa tagaggcggt cgctggtgtc gaagtcggtg gacacggttt cggggttgca
     2941 gttgaccatg atggtttcaa agcccgattc gcgcagggcg agtgcggcgt gaacgcagca
     3001 gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg ccgccgagaa tcatcacttt
     3061 tttacggtcg gaaggacggg attcgcattc ttcttcgtaa gtggagtaaa gataggcggt
     3121 ttcggtggcg aactcggcgg cgcaggtatc gacgcgtttg taaaccggat gcagcttcag
     3181 cgcgtagcgg tgttcgcgaa cttctttttc gcttacgttc aacaattgtg ccaaacgttt
     3241 gtcggagaag cctttgcgtt tcagacggcg tagggcggca taatccaaat cactcaaaac
     3301 gccgtctgaa accgcttttt cttccttcat caagtcttcg atttgcgcca agaaccaagg
     3361 gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag ccggcgcgga acgcgtctgc
     3421 cacaaacagc atacgctcag ggccggggtt cgccagttcg cggcggattt ccgctttgtc
     3481 ggagcttctc ggattgaagc cgcacaagcc ggtttccaag ccgcgcaagg ctttttggaa
     3541 gctttcctga atcgtgcggc ccatcgccat cacttcaccc accgatttca tctgtgtggt
     3601 caggcggtcg tctgcggcag ggaatttttc aaacgcgaaa cgcgggattt tggttacgac
     3661 atagtcgatg gacggctcga acgacgcggg ggttttgccg ccggtgatgt cgttgcgcaa
     3721 ctcgtccagc gtaaagccga cagccagctt cgccgccacc ttcgcaatcg ggaagcccgt
     3781 tgctttggat gccagcgcgg acgaacggct cacgcgcggg ttcatctcaa tcacaatcat
     3841 ctcgccgttt tcagggttta ctgcaaactg aacgtttgag ccgccggtat ccacgccgat
     3901 ttcacgcaac actgccaacg aagcgttgcg catgatttgg tattccttgt ccgtcagcgt
     3961 ttgcgccggc gcaaccgtaa tcgagtcgcc tgtatgcacg cccatcgggt cgaagttttc
     4021 aatcgagcag atgatgatgc agttgtcgtt cttatcgcgc accacttcca tctcgtactc
     4081 tttccagccg agaacggact gctcaatcaa cagctcgtgc gtgggcgacg catcgaaacc
     4141 gcgttcgcaa atcgccaaaa actcgtcttt attgtaggca atgccgccgc ccgaaccgcc
     4201 catggtgaaa gaaggacgaa tcagggtagg gaagccgacc tgttcttgcg ccgccaaagc
     4261 ttcgttcatc gtgtggcaga caaaagattt cgggcaggag aggccgattt tctccatcgc
     4321 ctccttaaag cggccgcggt cttctgcttt gtcgatggcg tcttcggtcg cgccgatcag
     4381 ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa tccagcgcac agttcagcgc
     4441 ggtctgacca cccatcgtgg gcagaatcgc atcaggccgc tctttggcga taatcttctc
     4501 caccgtctgc cacataatcg gctcgatgta ggtaacatcc gccatttcag ggtcggtcat
     4561 aatcgtggcg gggttggaat tcaccagaat gactttatag ccttcttcac gcaaagcctt
     4621 gcaggcctgt gcgcccgaat agtcaaattc gcaggcctga ccgataacga tagggccggc
     4681 gccgatgata aggatggatt ttaggtcggt acgtttgggc atgggtggtt actcttgaat
     4741 taaagaaggt tgttttgtta catagctttt aagaaaagta tttccctttt ctaaaattga
     4801 agctaaaaca tcatcttcta tgttagataa aatattctct aaatctgcgc ttgatttaat
     4861 tttaaagaat tcttttgttt gagaatggta tacaagagaa ggtttattta gctgtaaaag
     4921 ttctgctata ttatttaatg aaccaatact cttaccatcc cataaaataa agccataatc
     4981 agcaatttct gccattttct tatcttttgc tgtataaaat acccttccag ttcctttgct
     5041 gtctacttgg acaaactgcc aattccccac attatttcgg taaattttgc cagaaaagta
     5101 aatatgcaca ttagcataat cttgttcttg tagaaatttt tggatagctt tatcagcccc
     5161 attagcatct ccaataacaa tatcaaaatt attgctaaga atattgttaa ttctttctcg
     5221 tatttgtgga tttagtcgtg aaatgctgcg tgaaccagaa aagaaaatag tgcgcatttt
     5281 tatcctctag tctttgtcat tgctaaaaca attacagatc tagcattttt tgaaaacaat
     5341 ttttcagttg atatttccaa tgttgctccg ctatcaaata aatcatcaat tactaaaata
     5401 ttcttattgg atagatctat attgtcaatt gtgatggaat tattcagtat ttctagtttt
     5461 tctgattttt cttcaatatt tttgagtggt gtgtgtgagc tagattttct taaaatagga
     5521 gaatatggaa tattcaagcg atcacttagt tctttggcaa ttaattgaac tggttgatta
     5581 atacgctctg ttgtaaaagg ggcgggaaca attaaattta tagattctaa accactaaat
     5641 ttttgtaaaa tatagtctac cagcaaagaa acattttggg tttgattacg atattttaat
     5701 tgataaaccc attctccaat cacacttctc ttgctatcaa agtgaggatg ccctaattca
     5761 tcataaccta acaaaatact tttttgcata tgatggtcta aagcaaatcc ttttgtccag
     5821 ttaccattta gttctatcgc catttcattg actccttgtc aaaattaagt ataacgagca
     5881 atcctagcaa gccgtagccc gcatgtaggg tatgtgcggt acgcacgcac gcgttctcca
     5941 ttttccctgc aaccccaacc caccgcgtgc gtgccttgcg gcacactctc tgccgatgag
     6001 ttcaaaggtc gtctgaaaaa acgttcgcgg cgtttgttac atctgccttg cgataaaggc
     6061 tgcctgaaaa attcccactg ttgatatttc gggcattctt tctcaaaccg cctgcaaccg
     6121 caaaatccgc tgcaatctgc cccctccccc gtgggggagg accggggaga gggcattctt
     6181 cgaatttcgg caacctttcc caacaactca accaccgaaa tacaagcctt gtggcttgtt
     6241 gccttctctc tagctctctc ccacgggaga gaggacggag tggctgtagg gctaagggtt
     6301 ctgcaaggaa acaggttgtg caggttgctt tttgcttttc agacgacctg aaatcaacgg
     6361 taaatccaag tcgcttttca aaccgcctgc aaccgcaaaa tctgctgaaa cctactccct
     6421 cccccgtggg ggagggctgg ggagagggca ttctccgaat ttcggcaacc tttcctaaca
     6481 ctctaaccgc caagacacaa gccttgcggc ttgttgccct ctctctagct ctctcccacg
     6541 ggagagagga tggggtggct gttggggcta aggtttctgt aaactaatca ggttatgcag
     6601 gctgcttttt gtttttcaga cggcctttat aaatgctcaa ttcagtcggc tttatcctat
     6661 tattttcaat aatccaatca aaatcagcca tctgctatcg tgcagcttgc ttttccaatt
     6721 cttgcaacac gagcaggatt tctgtcagca catcgtttgt ctgctgcaaa atttcgtgat
     6781 tccaaaaacg cagcacggta aagcccaggc tgttgagata tgccgtccgc gcgtggtcgt
     6841 atacggcttg ttctgtgtgc tgccctccgt ctgcttcgac aatcaacttg ggcgttacgc
     6901 acataaaatc aacaatatag tttcccatcg gctgctggcg gcggaattta tagccgttca
     6961 gacgacctgc ccgcaggtgc tgccacaatt ttgcttccgc cccgctcatt tcttggcgca
     7021 tggctttggc gcgttggcgc aagatggggt tgtcggtggt caagagtttc tcgtgcgggt
     7081 tcattttgtt gtcgtgctgt ttgctgccct ctctctaact ctctcccacg gggagagagg
     7141 acggggcggt tgttggggtt aaaggggctg taaggaaaac aagtcgttca ggccactttt
     7201 tacttttcag acgacctgca atcaacggtt gtaaatccaa gttgcttttc aaaccgcctg
     7261 taaccgcaaa acttgctgca acctaccccc tcccccgtgg gggagggctg gggagagggc
     7321 attcttcgaa ttgcggcaac cttccccaac accctaaccg cccaaacaca agccttgctg
     7381 tttgttgccc tctctctaac gctttcccac agggagacag gacggggcgg ctgtagtgat
     7441 taagggtttt gcaaggaaaa caaggaaaac aagttgtaca ggctgttttt tatttttcaa
     7501 gcgacctgaa atcaacgctt gtaaatccaa gtcgcttttc aaactgcctg caaccgcaaa
     7561 atctgctgca acctaccccc tcccccgtgg gggagggctg gggagagggc attttccaag
     7621 ttgcggcaat ctttcccaaa tcccttagtc ttctaaaata caagccttac ggttgttgcc
     7681 aaatgaaact tcttccatac ccaaaacttg cgtgtaaaac gcaatggttc agtcaatgtc
     7741 ggcaacggtc agtaccagat ggtcgagtgc gctaatcttc atttcccgct ttctttttca
     7801 ggcggcggca gtgccgtctg aaagccatta tgcccgtttt gccgctttca tattgccaat
     7861 gaatttgtca aacaaatagc cgacatcttg cggaccgggg ctggcttcgg ggtggccttg
     7921 gaagcagaac acaggtttgt cggtcagctc gatgccttgc aaagtgttgt caaacaagga
     7981 tttgtgggta atgcgtgcgt tagcgggcag ggtgtcggca tcaacggcaa aaccgtggtt
     8041 ttggctggtg atgacgactt tgccgctgtc caaatcttgc acagggtggt tcgcaccgtg
     8101 gtggctgaag cgcattttca gggttttcgc gccgatagcg aggctgatga gctggtgtcc
     8161 caagcagatg ccgaaaatcg gtttgccgct ttccatcagt ttttgcacgg cttcgatggc
     8221 gtaggtgcaa ggctcggggt cgccggggcc gttggacagg aacacgccgt cgggattgag
     8281 tgccaacacg tcttgtgcgc tcgtttgggc ggggacgacg gtcaggcggc agccgcgcga
     8341 ggcgagcata cgcaggatgt tggttttcac gccgaaatcg taggcgacga cgtggtaagg
     8401 ctgttcgtca ggggtaacga aacccttgcc caattcccat tcgccttccg tccattcgta
     8461 agtttccgtg caggaaactt cttttgccaa gtctttgccg accatgctgc cgaacgcggc
     8521 gatgagttct tgcgcttttt caaccgtagc gtccgcgccg gtcagaatcg cgccgccttg
     8581 cgcgcctttt tcgcgcaaca gcgtggtcag gcggcgggta tcgatgtcgg cgatggcgac
     8641 ggttttgttg cgtaccagat agtcgtgcag gctttcggag gcgcggaagt tgctgtgcaa
     8701 gagcggcagg tcgcggataa tcaggccggc ggcataaacg ctgcggcttt cttcatcttc
     8761 ggcgttggtg ccggtgttgc cgatgtgtgg gtaggtgagg gtaacgattt gtttgcagta
     8821 ggacgggtcg gtcaggattt cctgatagcc agtcatcgaa gtattgaaca cgacttcgcc
     8881 ggaagtcgaa ccttcgtaac cgattgatgt gccgtggaat acgctgccgt cagcgaggac
     8941 gaggagggcg ggggtgctca tgatgggaat cctgttttta ataaaattct tgacaatgcc
     9001 gtctgaaggg acttcggcag ggggcatccg gtcggaaaaa tccggtcaaa aaaaaacacg
     9061 ccgcgcaaga tagacgcgta acgtgctttt cgggcaagtg cctataccct gaaagcagca
     9121 tattttaaga cgaaacatac ccgatttcaa gcgcaaacgc ggcaggcggg ctgatatgtt
     9181 cgcgcgaaaa cggaaatccg tggcgtaaaa ccgcccgctc tgcgcccact atgcccgtta
     9241 cgcatttccc cacgtccgtc cggcgaaact atgggaatac c
//
LOCUS       DAWKQJ010000071         9206 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000071 DAWKQJ010000000
VERSION     DAWKQJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.071"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJL20_001224"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJL20_001224"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ7683381.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJL20_001225"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJL20_001225"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ7683382.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3195..3896)
                     /locus_tag="WJL20_001226"
     CDS             complement(3195..3896)
                     /locus_tag="WJL20_001226"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ7683383.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4082..5194
                     /locus_tag="WJL20_001227"
     CDS             4082..5194
                     /locus_tag="WJL20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7683384.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5228..6205
                     /locus_tag="WJL20_001228"
     CDS             5228..6205
                     /locus_tag="WJL20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ7683385.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHGEALAYLRDRGVAEPEEFLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6209..6559
                     /locus_tag="WJL20_001229"
     CDS             6209..6559
                     /locus_tag="WJL20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ7683386.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6564..7343
                     /locus_tag="WJL20_001230"
     CDS             6564..7343
                     /locus_tag="WJL20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7683387.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRPV"
     gene            7369..7598
                     /locus_tag="WJL20_001231"
                     /pseudo
     CDS             7369..7598
                     /locus_tag="WJL20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            7699..8010
                     /gene="grxD"
                     /locus_tag="WJL20_001232"
     CDS             7699..8010
                     /gene="grxD"
                     /locus_tag="WJL20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ7683388.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8116..8742
                     /gene="upp"
                     /locus_tag="WJL20_001233"
     CDS             8116..8742
                     /gene="upp"
                     /locus_tag="WJL20_001233"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ7683389.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8765..9085
                     /locus_tag="WJL20_001234"
     CDS             8765..9085
                     /locus_tag="WJL20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ7683390.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         2242 a         2369 c         2396 g         2199 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg ccggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgcggaaca tatccgtgtt gtggatttcg gaagggtcgg tatcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggctgcaga ggataattaa aaacaacgac atcgccccgt tcgattttgc ctgtaggaat
      661 aaatatattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgccctta atcaggcccg ggcgcatcga gctggacggg atttggaacg gttcggcaat
      781 aaacgaccgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt gttccttaat gcttaaaaac
     1141 ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat cagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gccaaaacgt ttttacgcag ggctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcgatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat ttttgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtgcacaa
     1561 tcaggcttaa tgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcaaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatcactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttctcaccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccgactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgattttggt gtgaatacgc
     2341 ccagctgctc gacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacgat aaccgcttgc agcggcgcgt tttcgtcgcc ggtcggcgca gggattttgg
     2521 caacaatttc ttccaaaaca tcttccacgc cgataccgct tttggcggaa cactgtactg
     2581 caccgacggc atcgatgccg atgatgtctt cgatttcctg ttccacacgc tcggggtcgg
     2641 cggcgggcag gtcgattttg tttaaaacag gcacaacctc cacgcccaaa tcaatcgcgg
     2701 tgtaacagtt cgctacggtt tgcgcttcca cgccctgcga cgcgtcaacg accagaagcg
     2761 cgccttcgca agccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc tttatagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aaaccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagttg
     3061 ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga acaaacggcg ttgcgtccga
     3121 atatcgggtg caacgtggaa atagcccgat attctaacgg aaaaccgctg ttttggcatg
     3181 atttgataaa ggttttataa agatttgacg atttctgcca ccatttttgc agaacttgcc
     3241 gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct tttcgtccgc cgaatcggaa
     3301 accgcgcgga tgatgacgaa aggcgtttcc aactgatgac aggtttgggc aatcgccgcc
     3361 gcttccattt ccactgcttt gactttgggg aagtgcttgc ggatttccgc cacgccttcg
     3421 ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt gttctaccgc cgcgccttca
     3481 aacacttccg ccgcctgttt tgctttgccg accaagaggc cgtctgaaac aaatacggcg
     3541 ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg ttacatcgac atcgtggtgc
     3601 gcgatttctg tgccgaccac gacatcgccg actttcaaac ccttgcccaa gccgcccgcg
     3661 ctgccggtgt tgataacgca gtccggtgcg aattgatgga taagccaagc cgttgcaacc
     3721 gccgcgttga ccttgccgat gccgctcaat gcaagcacca tgcgttttcc cgctaattcg
     3781 ccttcatagg cggcaaactt gccgaaagag acggctttga cattttccat catctcgcgc
     3841 aaaagctcga tttcttgttc cattgcgccg ataacggcta ctgttttcaa agacatattg
     3901 ctgacctgtt gtgaatttcg gatagaatgc ctgattatac acgctaacac ggcaggattg
     3961 agtggaggtg gtttgtccgt gccgtctgaa acggtttcag acggcacggc gggtttttgg
     4021 tagaatggga aggtacagat tgtttgaaga ttaggggacg aggatgttta ccgatgaaaa
     4081 tatgaccgca aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
     4141 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
     4201 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
     4261 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
     4321 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
     4381 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
     4441 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
     4501 atcgacttcg cttgcctcgc ttatcgatta ccgcaatgaa aattcgttcg gacatatcat
     4561 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
     4621 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
     4681 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acgatggact acgccattgc
     4741 ctttgccgaa acggggcatt tgtgtatggc gacgctgcac gccaacagca ccaatcaggc
     4801 actcgaccgc atcatcaact ttttccccga ggagcggcgc gaacaattgc tgacggattt
     4861 gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt ccgcgagacg gcggcaaggg
     4921 cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg atttcggagt tgattcacaa
     4981 cggcaacatc catgaaatca aagaagtgat gaaaaaatcc actaccctgg gtatgcagac
     5041 cttcgatcaa cacctttacc aattgtatga aaaaggcgat atttccctgc aagaagcatt
     5101 gaaaaatgcc gattccgcac acgatttgcg tttggcggta cagttgcgca gccgccgcgc
     5161 gcaaagttcc agccccgatt tggaactgct ctgatggcgg tatggatttc cggacggatg
     5221 gtttgaaatg atttatccgt ggcataatga gcaatggcgg cagattgcgg aacattggga
     5281 gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat acggggaaaa ctacatttgc
     5341 ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca ccgggctgca aaccctgtgg
     5401 cgaatgtatg tcctgccatc tgtttggaca gggaagccat cccgattttt acgaaatcac
     5461 ccccttgtcg gacgaacccg aaaacggacg caaactgttg cagatcaaaa tcgatgccgt
     5521 cagggaaatc atcgataatg tgtacctgac ttcggtacgg ggcggtttgc gcgtgattct
     5581 gattcatcct gcggaaagta tgaatgtcca agccgccaac agtttgttga aagtgttgga
     5641 agaaccgccg ccacaagtgg tctttttgct ggtcagccac gcggcggaca aggttttacc
     5701 gaccattaaa agtcgctgcc ggaagatggt tttgcccgct ccttcgcatg gggaagcatt
     5761 ggcatatctg cgcgacaggg gtgtggcgga gccggaagaa tttttggcgt tccattcggg
     5821 tgcgccgctg tttgatgagg cggacggtgt ccgtgcgttg cggattaaac tgttggatat
     5881 tttggcagaa ccaaggttgt tgaagatttt ggattacgcc gcgcttttcg ataaggagaa
     5941 acttccgctc gacgtatttg tcgggtggat gcagaaatgg ctggtcgatt tgggattgtg
     6001 cctgcaacac atgaaacccg tctattatcc cgcttatgaa gacaggctgc ttcagacggc
     6061 atccggtttc cgcccgcgca atgtatttgc ggcggaggat atgctcaaac agcttgcccc
     6121 ttacgggttt catactttaa atgtcaaaat gcagatcgag catctgctca tcaattattt
     6181 ggaattgaag aaagagaacg ggtgaattat gtcagacgga caaaatattc cggcaaaaat
     6241 gatgtcgttg cagctgaaag acatgaatct gctgtacagc tcctatatgc cgtttttgga
     6301 acacggcggt ctgtttgtgc agaccaacga cgtattttcc atcggggacg atattctgct
     6361 tgccgtagaa atcctcaact tccccaaact gttcctgccg accaaagtcg cctggatcaa
     6421 tcctgcgcgt acttcctcca aacccaaagg ggtggggctg gcattcacaa aacacgaaaa
     6481 ctgcctgaaa gtcaaagacc agatcgaagt cgaactgggc aacacaatcg gcggcagcag
     6541 acctacgttt accatgtaac gccatgcata tcatcgattc gcactgccac ctcaattttg
     6601 aaggtttgaa agaacgcctg cccgaagttt tgtccaacat ggaagcaaac ggcgtggggc
     6661 aggcgctcgc catcagcgtc agcagggaaa gttttgccga agtatccgct atcgccgaag
     6721 tttatgaaca tatttactgc acaataggcg tacatcccga cagcaaggaa gccgaagaat
     6781 tttctattgc ggaaatggtt gctgcagccg cccatccgaa agtggtcggc atcggcgaga
     6841 cgggcttgga ttattactgg tgcaaaggcg atttgtcctg gcagcacaaa cgctttgcag
     6901 accacatcga agcagccaat caaaccggac tgcccgttat cgtccatacg cgtgatgcgg
     6961 cggcggacac cttgtctatc ctgaaagaat gccgggttaa ttcgggcgtt atccactgtt
     7021 tttccgaaga catcggtttt gcccgtgcag caatggattt ggggctttac atttctttct
     7081 cgggaatcgt tacctttaaa aacgccccct tggttcagga ggcggcaaaa tacgttccgg
     7141 acgaccgcat tttggtggaa accgatgcgc cattcctcgc ccccgttccc aaacgcggca
     7201 ggcagaacga accggctttt gtgcgccata ccgccgaaca tatcgccaaa ttgcggaacc
     7261 aaacattgga acaggttgcg gcatatacga cggaaaactt ttaccggctg tttaaaaaag
     7321 tacccgatat gcggcccgtc tgaccctgta ccgacgataa ggaaaaccat gaaggcaatt
     7381 catccgtatg catgtccgcg ctgctgccgg ctgcctgcca acacgtttcg gacaggcatg
     7441 aggcagtcgg tatggtgaag tccgcttcca aattctgcat tgccaaaggc ggcagacggg
     7501 aagtaaaaaa agacgaaagc ggcggcggat atgccctgtg ccatttgccg gacagtagga
     7561 ttgtcgagga gtgggaatat ttccgttcac aacattgata ctgcgcgata tacggcaaat
     7621 attgtgggaa gtttccgctt ttgcgtataa tgcgtcctac ctgacaaatt ttgtcaactt
     7681 tatcaaaagg aataagcgat ggcttccatc cacgaccaaa ttaaagaagt agtaacgaca
     7741 caccgcgtcg tattgtttat gaaaggtacg aagcagtttc cgcaatgcgg tttctcttcc
     7801 cgcgccgtgc aaatcctgaa cgcggcaggc tgcaccgatt acgttaccgt caacgtattg
     7861 gaaaatcccg aagtacgcca aggcattaag gaatacagcg actggccgac catcccccaa
     7921 ctttatgtga acggcgagtt tgtcggcggt tcggacatcc tgatggaaat gtatgaggca
     7981 ggcgagctgc aagagctgct gaaagcctga tggattcggc aatgccgtct gaacgtgttt
     8041 cagacggcat tttcttttcc ggcaaatcaa aaaaaagtat aatggcgcgt ctcaaaatca
     8101 cactggaaca ccgcgatgaa cgttaatgtt atcaaccatc cgctcgtccg ccacaaatta
     8161 accctgatga gggaggcgga ttgcagcacc tacaaattcc ggacgcttgc catcgagctg
     8221 gcgcgcctga tggcatacga ggcaagccgt gattttgaaa tcgaaaaata ccttatcgac
     8281 ggatggtgcg gtcagattga aggcgaccgc atcaagggca aaacattgac cgtcgttccc
     8341 atcctgcgtg caggtttggg tatgcttgac ggcgtactcg acctgattcc gactgccaaa
     8401 atcagcgtag tcggactgca gcgcgacgag gaaacgctga aacccgtttc ctacttcgag
     8461 aaattcgtgg acagcatgga cgaacgtcct gccctgatta tcgacccgat gctggcgaca
     8521 ggcggttcga tggttgccac catcgacctg ttgaaagcca agggctgcaa aaacatcaag
     8581 gcactggtgc tggttgccgc gcccgagggt gtgaaggcgg tcaacgacgc gcaccctgac
     8641 gttacgattt acaccgccgc gctcgacagc cacttgaacg agaacggcta catcatcccc
     8701 ggcttgggcg atgcgggcga caagattttc ggcacgcgct aactgactga ttttcggagt
     8761 tgatatgaat tttcaagact atctcgccac atttccttca atcgaccatc tgggcggttt
     8821 ggacgttcag gatgccgacg gcaaaacggt tcaccacatt cccgccgttc agggtaagct
     8881 cggttcgctc aagctgtaca atgctttggc agaacgtttt gacggaaaat tggataaaga
     8941 agcggcagaa cagggtttga tatggtttgc cgaacacgtt gccgacgcgc gcgcccatcc
     9001 cggcaagcat cccaatatcg acctgcttga aaaagttgtg caaagcggcg aaaccttgct
     9061 gctcaagccg cttgccgcgc aataattttc gaccatgccg tctgaaatcc gtttcagacg
     9121 gcattttatc ggaaagaaga ccgtaaaacg ggcattttct tttctatttc aggatacggg
     9181 caatgatgtt tcaacacaca ggacga
//
LOCUS       DAWKQJ010000072         9076 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000072 DAWKQJ010000000
VERSION     DAWKQJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.072"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(4..1260)
                     /locus_tag="WJL20_001235"
     CDS             complement(4..1260)
                     /locus_tag="WJL20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ7683391.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQNLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1608..1949)
                     /locus_tag="WJL20_001236"
     CDS             complement(1608..1949)
                     /locus_tag="WJL20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683392.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2153..3010)
                     /locus_tag="WJL20_001237"
     CDS             complement(2153..3010)
                     /locus_tag="WJL20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ7683393.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3071..3886)
                     /locus_tag="WJL20_001238"
     CDS             complement(3071..3886)
                     /locus_tag="WJL20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7683394.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3950..4120
                     /locus_tag="WJL20_001239"
     CDS             3950..4120
                     /locus_tag="WJL20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683395.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4130..4915
                     /locus_tag="WJL20_001240"
     CDS             4130..4915
                     /locus_tag="WJL20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ7683396.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            complement(4896..5114)
                     /locus_tag="WJL20_001241"
     CDS             complement(4896..5114)
                     /locus_tag="WJL20_001241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683397.1"
                     /translation="MLIHYKQIAQGSPFNQIWVSILGSLGHLAVSTFLPPLPVKMPSE
                     ISARYFRRHTRPFSHIFGKGLKFKRVRK"
     gene            5185..7308
                     /locus_tag="WJL20_001242"
     CDS             5185..7308
                     /locus_tag="WJL20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7683398.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMDP
                     EGAVSGSVNIETKKAADEGNRKIGLGWFSNNRAQGTFDLGQRFGENKAFGVRANGKLR
                     HGDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETSPVTH
                     NWSTAFDRVIRQRKTIRGTAAGAGKVEVKANENIANQLASFKADYPNSWENSANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQP
                     DLVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKR
                     PGYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANL
                     LNNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLN
                     SNVSYFGKSYQDTQKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQ
                     RGQYDRSFAVVGMPRTYWLKAELDF"
     gene            7432..8982
                     /locus_tag="WJL20_001243"
     CDS             7432..8982
                     /locus_tag="WJL20_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ7683399.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPELDFDEA
                     GMVSEPQQ"
BASE COUNT         2277 a         2168 c         2388 g         2243 t
ORIGIN      
        1 ggtttatttg cgttgcaata cggcggcgaa gaagccgtcg gtttggtgct ttgccgaatc
       61 gaggcgcagg tattttccgg tatccaaatc aatcttcagg ttttgcagca gttcggcgca
      121 gttgacgggt tcaaattcgg gatgttcgga caggaaacgt tcgacttgca gctcgttttc
      181 ttcgggcagg atgctgcaag tggcgtaaac caagcggcct tgcggtttta ccagtttgga
      241 ggcagcatcg aggatgctgt gttgctgttc caaaaggttg gccacggtct cggcagattg
      301 gcggtatttg aggtcgggat tgcggcgtaa agtacccaaa ccggagcagg gcgcgtccac
      361 caacacacgg tcggctttgc ctgccagtcg ggcgatacgg ctgtcgtgtt cgctgctgat
      421 gcgttcgggg tggatattgg tcagtccggc gcgggtcata cgcggtttga ggttggcaag
      481 gcgtttttcg gcgatgtcga aggcgtagat tctgccttta ttggccattt gcgcaccgac
      541 agccaaggtt ttgccgccgg caccggcaca gaaatcgaca atgatttcgc cgcgttttgc
      601 gcccaccaat aaagcaagca gctggctgcc ttcgtcttga acttccagcg taccgtccaa
      661 aaacagctcg tgtttgttaa gcgcgatttt gtttttcagg cggatgcccc aaggcgaata
      721 aggcgttgcc tctgcatcgg cactttcggc ttgcaacaat ggcagcactt tatcgcgttt
      781 gcctttcaag gtattgacgc gtatatcgag cggcgcagct tggttgatgc tgcggccgaa
      841 ggcgaggatt tcttcttcgc gccaatgctg ctgcagctgt tctaccagcc attgcggcaa
      901 ttctgcggcg gtattcaggc cgtctgaaaa ctcggtttta cgggctttca aattgccgag
      961 aaactctatt tcttcttcat caagcaggtc tttgatttgg ctgatgttgg tgcttctgcc
     1021 gagaaccagt gcggcgagtg cggctttgcg cggctgcgcg tgcggacggc gcagggcggt
     1081 actgattttt tgatagtggc gcagcgcggc aaaggcggtt tcggcgattt cgtggcggtc
     1141 ttggcggccg agctttttgt gttcgcggaa ataggcggag aggacggcat cggaaggctg
     1201 tttgaaagtc agcatttcag ccaaaacttt ggcggtatgg tcgagttgtg cggcgttcat
     1261 aatgtaatgg gttctcttac tgtgggttat tcataatcaa tctaacgtcc ttaaaataac
     1321 aatagcatct ttatcattac ttagtattct atccgcaata agaaagatgt gttaaaagtg
     1381 cgcaatcggg atttgtctaa atttctccgt taactacaaa ggcagcctga aaaaagttct
     1441 attttcaggt cgcttgaaca gcatccgata taatcacatc agcgatttca ttttgtaatc
     1501 actccctaaa acatgaggaa aaaaacaaaa tttattttct tcgttcagta tatctatcct
     1561 aaatatgcca tcactccatt gagtttttaa tttgtatttc gctcttgtta aatgtttttc
     1621 tgtttgccga tttcttttgc taatgtaaaa aatactctac cactctcatt ggttaaagtt
     1681 ttcaagtatt catcaacatc agtaacatta ataacaactg tttcatttgt atgtttatta
     1741 aattctaaat caataataat attcccatta accaatattt catatatatc ttcttcttca
     1801 gggagcatag acaaacaata agcacttgaa gctgaaaact cttttttcag atactgaaga
     1861 agaaaagtat ttttaattaa aatattatta aatttatcta atgattgctt gacttcttta
     1921 gttgtccaat ttttaacatt attcttcatt gtattctacc tggggtcgta gttattaaat
     1981 taccatactt atttgtttgg atcgtcataa tattagttgg catgttgttg ttatatttat
     2041 catatccaat atccttatta actcggatga ctctttcata aattgtatgg gaaatgagat
     2101 ttccatcact aagcctctca atagatatac gcgttaatgt tgggaaaggg tttcagacgg
     2161 ccttcataat gcgttcgatt tcgtgccaaa agccgtccgg ccgacattcc aaaacggcaa
     2221 tcaagccttg gtcgatacgg cggatttcgg ttgcgttcaa tgtttcggtt tcggcgacac
     2281 gcgcaggcgc gaggtaggcc atacggtcga gcagatggta gcggacttct tggcgtttaa
     2341 acccgtattc cgcccaatct tgaatataga tgccgcgcca accgtatgga ttaagcggtg
     2401 gctcaaaagc atggaaaccg tgtggaaaaa atccgatgcc gcgcacgatg gaaacgggtt
     2461 ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa gtgtgcggca
     2521 gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg gggtcgagtc
     2581 cgcgcatgtc ttcgagcctg cccgtgccgc cgccgtaata tttgcaggtc agctcgatat
     2641 ggtagggctt gccgttaagc cttaccacaa aatccgccgc gccttgcgta ttgccgtccg
     2701 aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg gcaaaccaaa
     2761 atgccagcag ttcttcggcg taaatgccga ggcggtgtcc gaacggggcg cgtcgggtga
     2821 ggtgttccgt cagcggcgcg ggatcggcat ccaatgccaa aagatagcgg aaaccgcgtt
     2881 cccccaatag ttcgcgcaca cccaattcgc aaccgctttg ccacaaaggc ggcgcagtca
     2941 gcagcgaggc gaggtcgcgg acgggttggc tggtgagctt ccaccataat gcgtctaggg
     3001 cgtaattcat gcttatccga atatcagtca tgccgtctga aaagctggaa atgttttcag
     3061 acggccttaa tcagtctttt ttatggatac gatggctgac ggtattgaaa aaaccgcgca
     3121 ggatatcgat gtcttcggtt tgcgtgttgg cgcgtccgaa caggctctgc atacggcgca
     3181 tcagacgctc gccgttgcgg cggttgaaaa agccgatgtc gttcatcacg ctttccatgt
     3241 gggcgaccat gcctttgatt tgctcgtggg tcgcagcgtg gtcttcttgt tgaagatggg
     3301 tcatgggcga accggtttgg ctgaagattt cgtagcacac gacctgcacg gcttgggcga
     3361 ggttgagcga gaaatagtcg ggattgccgt tgatggtcat cagtcggttg caggcttgga
     3421 cttcttcgat gctcaagccg aaagtctcgt tgccgaaaac cagtgccact ttctcgcctc
     3481 ggtttgcggt ctgcagtaat tcggatacca aatcgcgcgg ggtttgcagc ggcgcagtaa
     3541 tttcgcggcg gcggctggtc agggcgcagg cgatggtggt gtcggcaagg gcttcgtcca
     3601 aagaagcggc aatggtggca ttttccaaaa cgtctgccgc gccggaagcg aggatgaagc
     3661 tttcttccgg taatttaaac gattgaggat gctccgggtc aaacacgggc gggttttccg
     3721 tcatcggcgt tgccatcaga tttggggcga cgatggtcag tttgtgcaga cccattgttt
     3781 tcatcgcgcg cgcggcagag ccgatgttgg cgggatggct ggtgcgcgtg aggatgatgc
     3841 ggatgttgtc cagataagcg ggcagggcgg gtttgagagt agtcatgttc ggttttcggt
     3901 cggaatggaa tagggtataa tgcgccgtct ttttcgttcg gacggtcaaa tgccgtctga
     3961 aatgttcttt aacaacgtcg gaaatttaac gttgcgcctg aagcagacac gcgtcgggcg
     4021 gtattttacc cgatttggca ggatttaccc caaccaaata aactccgatt ttgccgctcc
     4081 gaaggactgc gcgtccgaac cggcggtatg ccacccttaa ggaaatctga tgaatccgtt
     4141 tttgaataca gcctttaaag ccgcccgccg tgccggtcag atgatgatac gcgccgcggg
     4201 caatctcgat gccgtcaaaa ccgacagcaa agcattcaac gattttgttt ccgatgttga
     4261 ccgcaattcc gaaatcatcc tggttgaggc tttgaaagaa gcctatccgc accacaaaat
     4321 cacttgcgaa gaaagcggct cccacggtaa agccgcagcc gaatacgaat ggattatcga
     4381 tccgctcgac ggcacgacca acttccttca cggccatccc caatacgcca tctctatggc
     4441 actcctgcac aaaggcgtgt tgcaagaagc tttggtgtac gcccccgaac gcaacgacgt
     4501 ttacatggct tcgcgcggca aaggcgcgtt gctcaatgac cgccgcatcc gcgtttccaa
     4561 ccgcatcgaa ttgaaccgct gcctgatcgg taccggcttc cccgttgtcg atcaaagcat
     4621 gatggacaag tatctggcga ttttgaaaga tttcttggcc aaaaccgccg gcggccgtcg
     4681 tgaaggcgcg gcttctttgg atttgtgtgc tgtagcaaca ggtcgttttg acggcttttt
     4741 cgagttcaac ctcaaacctt gggacattgc cgcaggtgca ttgattgttc aggaagcggg
     4801 cggtatcgta accgatatga cgggtgagga cgcttggttg gaaagcggcg atattgtcgc
     4861 cgccaatccc aaagtattgg cgcagatgct gaaaatcatt tccgcacacg tttaaatttc
     4921 agccctttcc cgaaaatatg ggaaaacggg cgggtatgcc gtctgaagta tcgcgccgat
     4981 atttcagacg gcatttttac tggcagcggg ggcaggaagg tggaaacggc taaatgcccc
     5041 aaactgccga ggatggaaac ccatatctga ttaaacggag aaccctgtgc gatttgttta
     5101 tagtggatta acaaaaacca gtacggtgtt gcctcgcctt agctcaaaga gaacgattct
     5161 ctaaggtgct gaagcaccaa gtgaatgggc aaagccaacg cctttaccgc accgattacc
     5221 gtcgtcaatt acgacgaaca agccctcaat aacaccgaag cgcgtacttt ggtggatgcc
     5281 gtagcgaaaa aagacgcttc cgtttggcag ttcggcggcg aaagcaacac gctgaccgga
     5341 ctgtatttca gaggttatca gcttgatgcg cgccaattca gcgtcaacgg tttggcaggt
     5401 atgtacggca cgcaaggcac ggcaagcgtg caagttggat cggcacaact gattaaaggc
     5461 gcatccactg ccgtaaacgg catggaccct gaaggtgcgg tatccggttc cgtcaatatc
     5521 gaaaccaaaa aagccgccga tgaaggcaac cgcaaaatcg gtttgggctg gttcagtaac
     5581 aaccgcgccc aaggtacgtt cgacttgggc caacgtttcg gcgaaaacaa agcattcggc
     5641 gtgcgcgcca acggcaaact gcgccacggc gacaccccgc gccacggtta cagcgaagac
     5701 aacaaagaat ttgcggtaaa tgccgactat cgcggcgaaa cactgcgcgt agcgttcgat
     5761 tccatctacg caaaacgcaa aaccaacggc ggtcgcgcgc gtatgcagga cattcaaaac
     5821 gccggcggac gcttgtttgg cgcgcccgat ggtaaaacca acctgctgcc ttcttggaac
     5881 tggcaaaata ccgccggcca aaccaatatg cttacgtttg aatgggatgc gtttgataat
     5941 gcacagatta caggcggtat cggctacaac aaggcacgct attacggcac gctgatttcc
     6001 ccgaccgtat gtttgaatgc taccagcact tgtaccgata tgcaaaacac aaacgccagc
     6061 ggcaaagtaa cggcacgcaa ctatgactac aataccggta ctgcgcgcct gaccgaccaa
     6121 tatttccgca cattgagtat gaacctgacc gcacgcggcg aatttgaaac cagcccggta
     6181 acacataact ggagtacggc tttcgaccgc gttatccgcc agcggaaaac cattcgcgga
     6241 acggcagccg gcgctggcaa ggtagaagtg aaggcaaacg aaaatattgc gaatcaattg
     6301 gcatcgttca aagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat
     6361 atcaaagtca acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgc
     6421 ctgactttgg gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacagagc
     6481 ggcgatgcca aacgcttcag cccgatgctg atggcggcgt gggtgccgca acctgatttg
     6541 gttgtgtacg gcaactatat ggaagacttg gagcctgccg atatcaaaac cgatgattcc
     6601 ggcgaaacga caatggcgaa accgcgtgtc agccgtcagt ttgaagtggg cgtgcgtaaa
     6661 aactggggca atttcgtaac cacgttaaac gcgttccaaa tcaaacgccc gggctactgg
     6721 cgcggtcata caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac
     6781 aattccgatt tcgcccgcta taaggcgcaa ggcggcgcgg ccggcgacga gcaaggtatg
     6841 gaacgcaatc gcggtattga gttcaacacc tacgcgaact tgctcaacaa tactttgcgt
     6901 ccgactttgg gtctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat
     6961 atgctggtta acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaggc aggtttggaa
     7021 tgggatacac cgtttgccaa aggcttgact ttaaacagca atgtttcgta tttcggcaag
     7081 tcttaccaag acacgcaaaa acaatacgct ttcccgtctt acactttggt tgacgtaggc
     7141 gcgcgctaca aaaccaagct cggcaaaaat acgttgaccg tcagcagctc ggtggaaaac
     7201 ctgttcaaca aaaactactg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc
     7261 gtcggtatgc cgcgtactta ctggttgaaa gcggaattgg atttctgatt tctccgcatc
     7321 cggaaaatgc cgtccgaaca ggtttcagac ggcatttctt ttaaagaaat atggcgggat
     7381 atgcttaaat aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa
     7441 cctttgattt cggttgcggc agcattgctc ggcgttgctt tgggtacacc ttattatttg
     7501 ggtgtcaaag ccgaagaaag cctgacgcag cagcaaaaaa tattgcagga aacgggcttc
     7561 ttgaccgtcg aatcgcacca atatgagcgc ggctggttta cctctatgga aacgacggtc
     7621 atccgtctga aacccgagtt gctgaataat gcccgaaaat acctgccgga taacctgaaa
     7681 acagtgttgg aacagccggt tacgctggtt aaccatatca cgcacggccc ttttgccggc
     7741 ggatttggca cgcaggcgca cattgaaacc gagttcaaat acgcgcctga aacggaaaaa
     7801 gttttggaac gcttttttgg gaaacaagct ccggtttccc ttgccaatac cgtttatttt
     7861 aacggcagcg gtaaaatgga agtcagtgtt cccgccttcg attatgaaga gctgtcgggc
     7921 atcaggctgc attgggaagg attgacggga aaaacggttt atcaaaaagg tttcaaaagc
     7981 taccgcaaca gctatgatgc gcccttgttc aaaatcaagc tggcagacaa aggcgatgcc
     8041 gcgtttgaaa aagcgcattt cgattcggaa acttcagacg gtatcaatcc gcttgctttg
     8101 ggcagcagca atctgacctt ggaaaaattc tccctagaat ggaaagaggg tgtcgattac
     8161 aacgtcaagt taaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc
     8221 aatcccaacg gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc
     8281 aagaccgggg aatcaggctc atttatcaac agtgaagggc agttccgttt tgacacgctg
     8341 gtgtacggca atgaaaaata cggcccgctg gacatccata tcgccgccga acatctcgat
     8401 gcttctgcct taaccgtatt gaaacgtaag tttgcacaaa tttccgccaa aaaaatgacc
     8461 gaggaacaaa tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc cggactgttc
     8521 accaacaatc ccgtattgga cattaaaact tttcgtttca cacagccgtc gggaaaaatc
     8581 gatgtgggtg gaaaaatcat gtttaaagac atgaagaagg aagatttgaa ccaattgggt
     8641 ttgatgctga agaaaaccga ggcagacatc agaatgagta ttcctcaaaa aatgttggaa
     8701 gacttggcgg tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa
     8761 ggcagggcaa gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt
     8821 cagagtatgg caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt
     8881 tctctgaaaa acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagctg
     8941 gattttgatg aagccggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg
     9001 aaatgttcag gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat
     9061 aagccgtgtc ggattc
//
LOCUS       DAWKQJ010000073         9051 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000073 DAWKQJ010000000
VERSION     DAWKQJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.073"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(176..868)
                     /locus_tag="WJL20_001244"
     CDS             complement(176..868)
                     /locus_tag="WJL20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7683400.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(976..2169)
                     /locus_tag="WJL20_001245"
     CDS             complement(976..2169)
                     /locus_tag="WJL20_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7683401.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2298..3035)
                     /gene="pxpA"
                     /locus_tag="WJL20_001246"
     CDS             complement(2298..3035)
                     /gene="pxpA"
                     /locus_tag="WJL20_001246"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ7683402.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3022..3951)
                     /locus_tag="WJL20_001247"
     CDS             complement(3022..3951)
                     /locus_tag="WJL20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ7683403.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3948..4595)
                     /gene="pxpB"
                     /locus_tag="WJL20_001248"
     CDS             complement(3948..4595)
                     /gene="pxpB"
                     /locus_tag="WJL20_001248"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ7683404.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4779..6986
                     /gene="uvrD"
                     /locus_tag="WJL20_001249"
     CDS             4779..6986
                     /gene="uvrD"
                     /locus_tag="WJL20_001249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ7683405.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKIAAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7343..7801
                     /locus_tag="WJL20_001250"
     CDS             7343..7801
                     /locus_tag="WJL20_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7683406.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(8072..8287)
                     /locus_tag="WJL20_001251"
     CDS             complement(8072..8287)
                     /locus_tag="WJL20_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683407.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8295..8498)
                     /locus_tag="WJL20_001252"
     CDS             complement(8295..8498)
                     /locus_tag="WJL20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683408.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8411..8770)
                     /locus_tag="WJL20_001253"
     CDS             complement(8411..8770)
                     /locus_tag="WJL20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7683409.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8823..>9051
                     /locus_tag="WJL20_001254"
     CDS             8823..>9051
                     /locus_tag="WJL20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7683410.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWN"
BASE COUNT         2283 a         2485 c         2235 g         2048 t
ORIGIN      
        1 acctctttca gttacagcag cttgatccct ttcccttatc caacggggga aggctaggat
       61 agggtggctt gcaaatatac agaacaaggg acaagagcca ccctctctcc aaccctctcc
      121 ctccgtacgg gagggggtgg attctcgcgg gcgaagccca cgctacggtt agcctttacc
      181 ccagcacaaa caattcccgc ccgtgcgcct tcagccaact tttagcattg tcggtatgcg
      241 gcgtcagcgt gttcaccaaa tgccaaaagc gcggactgtg gtcggggtgg cggaggtggc
      301 agagttcgtg gatgcagaca tagtcggcga cgtattcggg cgtgccgatc agccgccagt
      361 tgaggcggat gccggtgtgc gggcggcata cgccccaaaa ggttttggcg ttgctcaggt
      421 ctgtggcggt gggcgtcagt cctgtttcgg ctgcgtgttt ttcaaggcgg ggcagcaggt
      481 attcgcgggc gcgttcgttc aacaggcggc gcaggtggtc gatttgtgcg gcggtttctt
      541 ttcggggaag caggatttca gacgacgtga tacggatatg gctttggctg tgggtatcca
      601 gcttggtctt tattccccga taccaaatcc actcgggtaa gtttgggtgg gaaacaggat
      661 gcacgggcgt tttggcaagc gtgttccgca aaatcgtttc gtttgccgcc agccagtttg
      721 ctaacgcgtg gtcttgaaaa aagggtggga cgttgatgct gaccgtctgc atattgacgg
      781 ggcgcagaat cagatttttc ttggcactgc gtttgagttc gatttcgatg cacaaaccgt
      841 cggaaagagt ataggtgaag cgtttcatag ttgtgaatgg gtttcagacc ggatacatcg
      901 tctgaaacag gaattttcca tatcaggcgg caaacttcgg ataatataca aaatcaaaca
      961 tctgcgctac aaggttcagc cgaacaagcc gccgatatat ttgctgatgg tgatggcgct
     1021 gagtactgcc atcaaaccga ccacaatcac gccggaaacg gtgagccaca gcgggtgttt
     1081 gtagtcgccg acaattttgg ttttgtaggc ggcaatcaga atcagaccga gggaaatcgg
     1141 taaaatcagg ccgtttaatg cgcctacgaa caccagcacc tgcgccggtt tgccgatggt
     1201 ggaaaatacg gcggtggaca cggcgataaa ggcaataatc catttgtttt tattgcgttc
     1261 gatagacggg ctgagaccgg agaagaacga caccgaagta taagccgcac caatcaccga
     1321 agtaatcgaa gccgcccaaa tcaccacgcc gaaaatcagc aggccgatgt atcccgccgc
     1381 atattcaaac ggtgtggaag cagggttgtc gggattgagc tgtacgcctt ggctgaccac
     1441 gcccaaaacc gccaaaaaca atacaatccg cataatcgag gcaatcagga tcgcccgcac
     1501 cgagctttgg ctcacttccg gcaacgccga tttgcctttg atgcctgcgt ccagcagacg
     1561 gtgcgcaccg gcgaaggtga tgtagccgcc gaccgtgccg cccaccagtg taacaatcgc
     1621 cattgcatcg agtttttccg gcataaaggt atgcacggcg gcatctgcca gcggcggatt
     1681 cgcctgccat gccacataaa ccgtcagcgc aatcattacg aaacccatca cttgggcgaa
     1741 tttgtccatc actttgcctg cttctttaaa cagaaacaca ccgatggcaa tcacgccgct
     1801 gatcacggca ccggtttccg gtgacagtcc ggtcagcagg ttcagaccca agcctgcgcc
     1861 gccgacgttg ccaatattga acgccaaacc gcccatcaca atcagcacag ccaagaaata
     1921 gcctgcgccg ggcaagacct gattggcaat atcctgcgcc tgtttttcgg aaacggcgac
     1981 aatccgccaa atattgagct gcgccccgat gtcgagcaga atcgagagca gaatcacaaa
     2041 gccgaaactt gccgccagtg cttgggtgaa ggtggcggtt tgggtcagaa agcccgggcc
     2101 gatggcggaa gtcgccatca ggaatgcagc gccgattaag gcatttctgc ggtttttttg
     2161 attagacata atcgcttatc ctctataaaa ttggttgttg ctgtgtttgg gcgaaacctg
     2221 cggttttagc tacgcagaaa ctcgctttgc tcgttttggc gaaacctgcg gttttcagac
     2281 ggcctatgaa ctgtttttca agcagaaact ttgatgcctg ccgccagtag ttcctgccgg
     2341 attttttcgg caaacaccac ggcgtgcggc ccgtctccgt gcagacagat gctgtcggct
     2401 tgcacggcaa ccaggctgcc gttcactgct ttgacccgcc cgtcccgcac catctgcaat
     2461 acttgggcga tggcttcttc gtcgctgtcc acctgcgcat cggggcggct gcggggaacc
     2521 agcgtaccgt cgggcatata gcggcggtcg gcgaatactt cggaaatcac acccaagcct
     2581 gcggcttttc cggcttccaa gagcaggctg ccggaaagtg ccatcaattt caatttcggg
     2641 tcgaaatccg ccacaattcg ggcaacggta tccgccagcg cacggttttt cgccgcttgg
     2701 ttgtacattg cgccgtgcgg tttgacataa gccatttcca aaccctgatc acggcacaag
     2761 gcctgcaatg cgcccaactg gtaattcaga cacgcccgca aatcggcttc ggacagattc
     2821 atttcggtac ggccgaagtt ttcccgatcg ggatagccgg ggtgtgctcc gatgcgcacg
     2881 ccgttttgtt gggcatacgc caatgccgcc cgaatatcgg caatgctgcc ggcgtgttgg
     2941 gcgcaggcga tgttggccga agtaatcagc tgcaacaagg cttcgtcgct gccgcagcct
     3001 tcggcgagat cggcgtttaa atcaacctgc ttcatgggtg attctccgta tttggttcag
     3061 atagacttgg tttttgcgcc gcagggcggt ggcttctttc aagccgatta ttttgaattt
     3121 gactttgctg ccgaagcgca cctgtgccag cctgcccaaa tcggcggcgg caacggtagc
     3181 gattttcgga taaccgccgg tggtttgcgc atcggccagc aggataatcg gtttgccgcc
     3241 gggcggcacc tgcacggttc ctgcctgaac agcgtgggac agcatttcca aaggttgcga
     3301 cagggtcagc ggctgtccgt cgaagcggta gcccatgcgg ttgctatcgc tttgcagcgt
     3361 ccacgtttcc cgttccagat tcagacgccc tttttcactg aaagcggcat attccgacga
     3421 aggaacaagg tggacggtat cggtaaacgg tatcggggca atgccgactt tggacaattc
     3481 ctgcgcacct ttgccgatgg ggagacaatc acctttttgc agcattctgc cctgatggcc
     3541 gccgaaaccg gctttcaggt cggtgcttct cgaacccatc acttccggca catcaaatcc
     3601 gcccgccacg cacacatagc cgtacatgcc ctgcacggca cgcaccattt tcaaggtctg
     3661 ccctttgcgg gcggtataac gccaatacga atagaccggt tcgccgtcca attccgcctg
     3721 atacacggca ccggtgagac aaaacggcgt atcccgttca aacaccagca ttatcccgcc
     3781 caaagcgatt tcgattgcgg ccgtgccttc gtcgttgccc aataaaatat tacccgccgc
     3841 caaagcaacc gtgtccatcg caccggcatg accgatgccg taacgccggt gtccgtagcg
     3901 tccggtatcc tgaatatgcg ccggtgcctg cactgccgaa acgtgaatca tggctcaatc
     3961 ctttctgcaa caaagcggac ttggtcaccc gccgccagca gggtcggcgg attcaaatcg
     4021 gctcggaaca agggtaattc ggttctgccg ataatctgcc agccgccggg cgaagcgaac
     4081 ggatacacac cggtctgact gccgccgata ccgaccgaac cggcaggaac ggacgttctc
     4141 ggcacggcac ggcggggcgt gtgcaatgct tcgggcaagc cgcccagata agggaaaccg
     4201 ggctggaagc ccatcataaa tacggtataa gtttgcgccg tatggcggcg gacgatttcg
     4261 gaaataaccg tctgatggaa agcagcgact tccgccaaat ccgggccgta ttcgccgccg
     4321 tagcagacgg gaatttccac cagtttgccc tgatggtctg taacggcggt gtgttcccac
     4381 acatattgca attcatcggc aagcgtcgcc aaatcggtat cgaaacgggt aaacacggtc
     4441 agattgttca tgccgaccac cacttcctca atcctgtcgt gctgcccgag cgcagcggca
     4501 aacgcccaca acttttgctg tttgcccagt tcggaaggcg cattcagtcg gcagaccaaa
     4561 gcggattcgc tgattggtgt gatctctatt ctcatttgtt gttcattttg gttatgtttt
     4621 aatgaatcta tatgcagggg cggcggtttg tcaatatctt ctgtgctgca tcatcaaacc
     4681 gtcgattgga aaagtgctgc cctgccgctg cactttttca gacgacctta aaccgtttct
     4741 attaaaatag cgcattccac ttttcagacg gcatccttat gtttcccgac caatccgccc
     4801 ccaacctgct gcaaggcttg aatcccgaac aactctccgc cgtaacctgg ccgccgcaat
     4861 ccgcccttgt actggcgggc gcgggcagcg gcaaaacgcg cgtgctgacc acgcgcatcg
     4921 catggctgtt gcaaaccggt caggcaagcg tgcacagcat tatggcggta acgtttacca
     4981 acaaagccgc caaagaaatg cagacccgtt tgggcgcaat gattcccgtc aacgtccgcg
     5041 ccatgtggct cggcacgttc cacggcctct gccaccgctt tctacggctg caccaccgcg
     5101 atgccggttt gccgtcatcc tttcaaatcc tcgacagcgg cgaccaactt tccctaatca
     5161 aacgtctgct caaaagcctc aacatcgccg aagaaatcat cgcgccgcgt tcgctgcaag
     5221 gctttatcaa cgcgcaaaaa gaatccggtt tgcgcgcttc cgtgttgagc gcgcccgacc
     5281 cgcacacgcg ccgcatgatt gagtgctacg ccgaatacga caaaatctgc caacgcgaag
     5341 gcgtggtcga tttcgccgag cttatgctcc gcagctacga aatgctgcaa agcaacgaaa
     5401 ttctgcgcca gcattaccaa aaccgcttca accatattct ggtcgacgag tttcaagaca
     5461 ccaacaagct gcaatacgcc tggctcaaac tcatggcggg cggcaacgcg gcggtatttg
     5521 ccgttggcga cgacgaccaa agcatctacc gcttccgcgg cgcgaacgtc ggcaacatga
     5581 ccgcactgat ggaagaattc cacatcgacg cgcccgtcaa actcgaacaa aactaccgct
     5641 ccgtcggcaa catccttgcc gccgccaatg ccgttatcga aaacaacgac gaacgactcg
     5701 gcaaaaacct gcgcaccgac gccgaagcag gcgacaaaat tcgctactac tccgccttta
     5761 ccgacctcga agaagcccgg ttcatcgtgg acgaaaccaa agccctcgaa cgcgaaggct
     5821 gggatctgga cgaaatcgcc gtcctctacc gtagcaacgc ccaatcccgc gttatcgaac
     5881 aaagcctgtt ccgcagcggc attccctaca aaatctacgg cggcttgcgc ttttacgaac
     5941 gccaagaaat caaacacgcc ctcgcctacc tgcgcctcgc cgtcaatccc gacgacgaca
     6001 acgccctctt gcgcgtgatt aacttcccac cgcgcggcat cggcgcacgc accatcgaaa
     6061 accttcagac ggcctcaaac gaacaaggca tcaccctctg gcaggcagcc tgcaatgccg
     6121 gcgcaaaagc caccaaaatc gccgccttcg tccgcctgat tgaagccctg cgcaaccaag
     6181 tcggacaact gtccctgtcc gaaatcatcg tcggcatcct caaagacagt ggcttgaccg
     6241 aacactaccg cacccaaaaa ggcgacaacc aagaccgtct cgacaacctt gacgaactcg
     6301 tcaacgccgc catcgagttc aaaccagaag acagcaactt tgaaaccctg cctgaaaaca
     6361 tttcagacga ccccgccttc cccatcctcg ccttcctcag caacgccgcc ctcgaatccg
     6421 gcgaaaacca agcaggcgcg ggcgaaaaag ccgtccaact catgaccgtc cacgccgcca
     6481 aaggcttgga attcaacgcc gtcttcctca caggcatgga agaaggccgc ttccccagcg
     6541 aaatgagcct cgccgaacgc ggcggcctcg aagaagaacg ccgcctcatg tatgtcgcca
     6601 tcacccgcgc ccgcaaacgc ctctacatca ccatggcgca acaacgcatg ctgcacggac
     6661 aaacccaatt cggcatcgtc tcccgcttcg tcgaagagat cccacccgaa gtattgcact
     6721 acctgtccgt caaaaagacc gcctacgaca gctacggcag cccgcgccaa accgccgcat
     6781 ccaaagacaa aatcatcgac gactacaaac agccccaaac ctacgcaggc ttccgtatcg
     6841 gacaaaacgt ccgccacgcc aaattcggca ccggcgtgat tatcgatgcc gcagataaag
     6901 gcgaatccgc ccgactgacc atcaacttcg gcaaacaggg cgtgaaagag ttggatacca
     6961 agtttgcgaa attggaagcg atgtaaattt gaaatgcagg ctggatactc gtatccgaca
     7021 aaaatatttg acgcgtctat cgtttccgaa aaaccgctgt tggaaaatgt cggattcgag
     7081 aatccgacct atggcaaaaa actgtagcaa ggacaaggca aaaggccgtc tgaatccggt
     7141 aacaacaaat tttgccgcaa ccgccgccgt cattcccgcg caggcgggaa tccagacctt
     7201 tcggcacggg aacttatcgg ataaaaggtt tctttagatt ccacgttcta gattcccgcc
     7261 tgcgcgggaa tgacggacgg taaaagccgg gtatagatac ctaacccttg tttatagctg
     7321 aaaatcaata aggaagaaca ttatgtccca agtttttaaa gattttgatt tgtcctccgt
     7381 atgggaatcc gacagttggg cagatgaaaa ctacaaagaa gccccgttta cccctgaaat
     7441 tttggctgcc gtagaaagtg aactgggcta taaattgccg caaagtttta ttgaattgat
     7501 ggcggtacaa aacggcggaa tatttgtcaa aaactgtttt ccgaccacac agagaaattc
     7561 gtgggcgaaa gatcatgtgc aaatttgcga ggtatcggga atcggttttg aaaaagaagg
     7621 aagcctgtgc ggcgaaacag ggcaaaaact ttggctggaa gaatgggagt atccgcccat
     7681 cggtgtgtat tttgccaacg acccgtcagg cggtcatgcc ctatttgcct tagactatcg
     7741 ggagtgtgga agagacggag agccgaaagt ggtgtttgtc gaacaagaat cggattttta
     7801 aatcgtcgaa cttgcccccg attttgaaac ctttattcac agcttgcggc atgaagatga
     7861 gtttattgac gaggaaatat aaaacggcgg ttgaaaaacc ggaatcatca agggaaaacg
     7921 ggcgaaataa cggataatcg cttgaatccg taaggaaaac ggtttggtgg aacgcgccat
     7981 ccaagacctt tgcaaagaaa aactgtctcc gacagcattg acattattaa cagaacttat
     8041 caagtttgga gctatctcaa atataattcg gttatccttt tgtatccatt aaatcatatg
     8101 cttcaattaa ttgttgttct agctcttgta ccaattttga attgcgaatt cctgacacaa
     8161 tctcaaattc ttctgcatct atgcaaacac ctgcataaat ttcaataaca agggaacgca
     8221 ataattgaag ctcttctctt gttaaagaaa taataatgtc atcacctgtg taattgatta
     8281 tattcataat aattttattt ttgtttgtca aagtaagttt tgcctaaggt tggtctaaat
     8341 gcagttccac catcttttga atttgggtct ctgattacaa ttgctccaga cttatcatcc
     8401 caaattgctc ttatgtgttt ggattgtaat cttcgaattc ccaagaaaaa aatcgtaata
     8461 agtttgaaag tgtcaaatcc caagtttctt ttgagcaata ttctaatatt ttatcaattt
     8521 cacttttaat aatcttatga tcaaactgtt ctaatattaa tgcattagac caaaaaaaac
     8581 cttctttatt acaatgatgg gaaatccatt taggagaaca aatgcaaagt gaaaaaatag
     8641 atgagccttg ttctccttcg attccgatat ccaaatctat ccatctatgg aaattatctg
     8701 gaatttcggg ggtaaatttt tcaaaatcaa tatcatataa atttatgctt tttaaatcca
     8761 atttaatcat tagggctgtc ctagataaat agggaaattc aaattaagtt agaattatcc
     8821 ctatgagaaa aagtcgtcta agccggtata aacaaaataa actcattgag ctatttgtcg
     8881 caggtgtaac tgcaagaaca gcaacagagc ctgacagcat tgtttatacg gattgttatc
     8941 gtagctatga tgtattagat gtgagcgaat ttagccattt ccgtatcaat cacagcacac
     9001 attttgctga acgacaaaac catatcaatg gaattgagaa tttttggaac c
//
LOCUS       DAWKQJ010000074         8656 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000074 DAWKQJ010000000
VERSION     DAWKQJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.074"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            169..1092
                     /locus_tag="WJL20_001255"
     CDS             169..1092
                     /locus_tag="WJL20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7683411.1"
                     /translation="MENQRPHLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WJL20_001256"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WJL20_001256"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ7683412.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2689..4668)
                     /gene="tkt"
                     /locus_tag="WJL20_001257"
     CDS             complement(2689..4668)
                     /gene="tkt"
                     /locus_tag="WJL20_001257"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ7683413.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKTETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5196..6692
                     /gene="ccoG"
                     /locus_tag="WJL20_001258"
     CDS             5196..6692
                     /gene="ccoG"
                     /locus_tag="WJL20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ7683414.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6695..7243
                     /locus_tag="WJL20_001259"
     CDS             6695..7243
                     /locus_tag="WJL20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ7683415.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(7303..8478)
                     /locus_tag="WJL20_001260"
     CDS             complement(7303..8478)
                     /locus_tag="WJL20_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ7683416.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
BASE COUNT         1859 a         2121 c         2509 g         2167 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat taaacgccaa acgcttgaac cattattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgcacc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcattgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggcgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt tttcgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaggc ttgggctttt ttgtccaagg
     2041 tgtcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatggc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgatacgcc gccaaacccg taccggcgat ttcgttggcg cggttcgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcgagcttg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcaac ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggcggaat gaggaagcat taaggctgga gggcaaaaaa
     2641 atgccgtctg aaaagcgttc agacggcatt tgatgcggcg gctgcggttt acagcacgga
     2701 tttcactgta tcgaccacat tgtccacggt aaagccgaat gctttgaaga gtaaatcggc
     2761 aggggcggat tcgccgaagc ggttgatgcc gacgactgcg ccgttcagtc cgacatattt
     2821 gtaccagccg tcggcgtggc cggcttctac ggcgatgcgc ggcaggcttt cgggcaggac
     2881 ggcggcttga taggcggcgt cttggcggtc gaatacgttg gtggacggca tggaaacgac
     2941 gcgcacggcg atgttttgcg cggcgagtgc tttttgcgct tccaaagcca gctcgacttc
     3001 ggaaccggtg gcaatgatga cggcttgggc gttgccttgg gcttcgctga tgatgtagcc
     3061 gccgcgtttg atgtcgtcca gttgttgttc gctgcgcgct tggaatttca ggttttgacg
     3121 gctgaaaatc aggcaggacg ggtgatcggc ggctttgacg gcttctgccc aagcgaccaa
     3181 ggattcggcg gtgtcgcacg gccgccatac gtccatattc ggaatcaggc gcagggtggc
     3241 ggtttgctca atcggctggt gggtcgggcc gtcttcgccc aaaccgatgg aatcgtgggt
     3301 gaacacaaat acagggttga ttttcatcaa cgcagccata cgcagggcgt tgcgctcgta
     3361 ttcgctgaac atcaggaaag tcgcgccgaa gggttttacg ccgccgtgca ataccaaacc
     3421 gttcataatc gcgcccatac cgaactcgcg cacgccgtag tggatgtagt tgccgccttt
     3481 gtcgcgggta acggaaacgc tgtttgacca gtcggtcagg ttggacgggg tcaggtcggc
     3541 agagccgccc accagttcgg gcagctcttt tgccaagatt tcgatgctgt tttggctggc
     3601 tttgcgggta gcgatggttt cggttttggc gcacacttct ttcaatgccg tctgaacgta
     3661 ctcgtcaaag ttttccggca gttttttatc catacggcgc acaaattctg cggcttcggc
     3721 aggatatttg gcttgatatt gcgcgaacag ttcgttccag tcggcttcca gtttcgctcc
     3781 cttttctttg gcattccacg catcgtaaat ttcttgcggg atttcaaagg cggggtaagt
     3841 ccagcccaaa tgtttgcgcg tggcttcgat ttcgtccgcg cccaaaggtg cgccgtgggt
     3901 tttgtggctg ccttctttgt tggcactgcc tttgccgatt aaggttttgc agcagatgat
     3961 ggacggtttg ccggtttcgg cacgtgcggc ttcgatggcg gcttgaatgg cggcggtgtc
     4021 atgaccgttt acgttaggaa cgacgtgcca gccgtagctt tcaaagcgtt gcgggatgtt
     4081 ttcggtaaac cagccgtcca ctttaccatc aatggaaata ttgttgtcat catataaaac
     4141 aatcagtttg cccaagccca aagtgccggc gagtgagcag gcttcgtgcg atacgccttc
     4201 catcagacag ccgtcgccca taaagacgta ggtgtaatga tcgacgatgt tcaaaccgtc
     4261 tttattaaat tcggcggcaa ggattttttc tgccaatgcc atacccaccg cgttggcaat
     4321 cccttgcccc aacgggccgg tcgtggtttc cacgccgtcg gtgtagccgt attcgggatg
     4381 gccgggggtt ttgctgtgca gttggcggaa gtttttcaag tcttcaatgc ttaggttgta
     4441 gccggtcagg tgcagcaggc tgtacaacag catagacgcg tggccgttgg agaggacgaa
     4501 gcggtcgcgg ttgtagaatt tggggttggc ggggttgtga ttgaggaatt tcgtccacaa
     4561 tgtttccgcc atttccgcca tacccatagg cgcgccgggg tggccggaat tggctttttg
     4621 aacggcatcg gccgagagga agcggattgc gtttgccagt tgagacattt tgtattttcc
     4681 ttgctggtgt ttcagataag tggataatcg gaaagcgttg attatcgccc gattcgctta
     4741 tgctttcaag aaaagggcgg acgcggggga aggcggcggc aggagaccgg tggggaagga
     4801 ttttcgattt gcgggcgaag cctgccatta ttccttttga aataaaaagt tatagattgt
     4861 gtgccggatt gtcgatagcg tttgtttatg agcttgcgcc gtcggttctg ccgatatggg
     4921 ggtgtcggtt ttttttagtc ttttttttaa ccgtattcgg attttgttcg ggcggtaagg
     4981 taaaatccag gcgttttgat gcggatggga aatgtatccg ccccatacat ccggacggcg
     5041 cataaagttg tacaatagcg gaaatatatt ttgggaaaaa cggattttct caaaacttga
     5101 aacacaacgc cttaaaaaac aagaaaaatg acgtctgaaa agcaaacggc ggcggtaacg
     5161 ggcaaaccat caatcaaaaa tctaaggaat gcagaatgac cacggaaaac caagccggca
     5221 gtccggcatc tggaatcggc acgtccgaac aaacaaaagc cgcgccgaaa gcaaaaaaaa
     5281 cattcgatcc gcgagcaagc gtgattcaaa tccatcccga aggcgaacgc atccatccca
     5341 aaaaggcgga aggacggttc gccaaactgc gtatcgccgc cgtattggcg acgcagtttg
     5401 tgttttacgt cattccgtgg ttcaactgga gcggcaggca ggctgtcgtt ttcaatatcc
     5461 ccgaacggca tttcttcatt ttcggattgt cgttgggggt gggcgatttg atttaccttg
     5521 ccttgctgct gatgatttgc gccttcgggc tgttttggtg gacgacgatt gccgggcgac
     5581 tgtggtgcgg ctattcctgc ccgcaaacgg tttacaccga aattatgctg tggatcgaca
     5641 acctggtcga aggcgataga aacaaacggc tgaaactgga aaaatcgccg tggaatttca
     5701 ctaaaatccg catcaaagcc accaaatacc tgttgatttt ccttgtctgt gcgtggacgg
     5761 gcatcacgtt tgcaggctgg tttgtcccta tccgccagtt cgttcccgat ttattcactg
     5821 gagcagcagg cggcggcgcg atgtttgcca cagcgtttta cggctttatg accttcttct
     5881 tcgcccacat tatgcgcgaa aaagtatgcc tgcatatgtg tccgtatgca cgtttccaaa
     5941 gcgcgatgtt cgacaaggac acgctgattg tttcttatga cgcggaacgc ggcgaaccgc
     6001 gcggcgcgcg caagaaaacg gtcaataagg aagaggcggg tttgggcgac tgcatcaact
     6061 gtgcgatgtg cgtccaagtc tgccccgtcg gcatcgacat ccgcaacggt ctgcaatacc
     6121 aatgtatcgg ctgcgccgcc tgtatcgacg cgtgcgatga gattatggac aaaatgggct
     6181 atccgcgcgg attaatccgt tatacgaccg aaagcgcgct ggaacacgaa tattctgaaa
     6241 aagacattaa aaaacggctg ctcagaccgc gcgtggcagg ttacggcgcg gtgttggcgt
     6301 tagttatcat tgccttcctg gccggtttgt ccacgcgcaa aatggtcgag gtcgatattt
     6361 tgaaagaccg tggcgtactg gtgcgcgaaa acgccaaagg ctggctggaa aacgcataca
     6421 gcctgcgtat catcaacaaa agtgaaaaag aacagctcat taccgcaagt gtcaaaggct
     6481 ttgacgaaat cgccctgacc gggctgcccg aaggcggtat caaggttgcc ccgcgcgaaa
     6541 cggtaaccct tcccgtccaa gtgtccacca ttccggaata cgcggacaaa ggcagccacc
     6601 ctatcgaatt taccttccaa taccgcgaaa gcggcgcgcc cgacggcaag ccggtcgtct
     6661 tggaagaaga tgcaaccttt atcggagaat aaccgtgtct caaaacactc caatcaaacc
     6721 ttggtacaaa cacgtctggc cgtggatctt gatggcgggg ccgatttttg tcgtcatcgc
     6781 cagcgtcgct atgttttttg tcgcgcagca gcacgcgaca gatttggtta cggacgatta
     6841 ttataaagac ggcaagcata tcgacatcca gcttcatcgg gatgaagaag ccgtcagacg
     6901 gcatatcggg gtgcaggttc tcatttcccc cgatatgaat gcggcaaaag tgtttgtcgg
     6961 cggcgagttt gacggcaaac agcctttgaa cctgctgctg atgcacccga cccgcaaggc
     7021 ggacgatcaa accgtcgccc tcaagcccgt cggcagcgcg cagaacggca gggcggaata
     7081 tgaggcggtg ttcaaaaccc tttcgccgac caaccactgg tatgtgcgcg tggaggacgc
     7141 ggcaggcgtg tggcgcgtcg agaacaaatg gattaccagc caaggcaatg cggtcgattt
     7201 gaccccgatg gacaaacttt tcaataatac tgaaagcaaa taaagagatg ccgtctgaaa
     7261 attttcagac ggcattttgc tacggtatcg gctgttttaa catcagcgtc ctactgttgc
     7321 aacaggttcg ctccccgtca gcagatattg tatcgtctcg cggacggtgc ggatggcatt
     7381 gccgaagggc agcgggtctt tacctctgaa gaacaggcct ttatctactt ctccacggaa
     7441 tgcggcggca agctggatat caatgcagaa ttgtccggct ttggaaagcc cgtcgcgcag
     7501 accgcaactg gttaggcagt ttaaaccttg ggtacagcgg cgcgggtcgg ctttggcgtt
     7561 tgtctgaagt ttgctttcac gcttgatgta gctgtctagg aatttggtgc ggacaccgcg
     7621 cgccggcaaa ccggcaacag acataaattc gactactttt tcagtttccg caccggcgag
     7681 cgtttttttg aagttaaggt gtgcatctcc ttcttcggta acggcaaaag ccgtaccgat
     7741 ttgaacggcg gatgctcccc agttctttag ggcggttttg actttttcaa aatttgccat
     7801 gcctcccgca agaataagcg ggattttttc gctttccagc cctaaacttt tgaaaacttc
     7861 aaacgtttcc tcaatcacgc gtttgaagtc gaacttggca tcgtttacgc cttcaacggt
     7921 tgatgcaccc aaatgtccgg ccgcgtgggc aggatgttcg actacaatcg catcgggcaa
     7981 tatgcctttt ttcatccaac gtttcaaaac gatattaata ccgcgcgatt cggacagaat
     8041 cggcagcagc gcgacatatt tatgatagcc ctcggtcatt tccggcaggt ctaaaggcag
     8101 accggcaccc attacaaccg catccgcccc tgattcgcaa gcctggcgga catatgcggc
     8161 gtggtctttg accgccttca tcacgttgac cgcaatcagc ccttttccct ctgaagcact
     8221 tttggctttt tggatttccc tgtccaatgc ggtacagttc aaagacgtat atttctcttc
     8281 actcggattg atttgggatt cggcgagtag gtcttcgtga aggtggcgca aatccacact
     8341 ggcaatcgtt ccgataccgt tttcacgtgc caccgcgctg gataaacccg atgcggaaac
     8401 accgaccccc ataccgcctt gcacgatggg ggtaagggat tttccacgaa taaccaaagg
     8461 gtcaaaaata ttctgcatca gtttctccga gtgtacgaat cagtcaggct catgaaaaat
     8521 ggttctaact gttttagaac tattgctcaa atttggtatt atactctaaa tatcgcggcg
     8581 gtaaacaaga aagcagccgg agcttcgggc atattctgtt acgcctgttt caatcgggag
     8641 agaaaatatt atagtg
//
LOCUS       DAWKQJ010000075         8596 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000075 DAWKQJ010000000
VERSION     DAWKQJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.075"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            164..1276
                     /gene="nadA"
                     /locus_tag="WJL20_001261"
     CDS             164..1276
                     /gene="nadA"
                     /locus_tag="WJL20_001261"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ7683417.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1328..2836
                     /gene="nadB"
                     /locus_tag="WJL20_001262"
     CDS             1328..2836
                     /gene="nadB"
                     /locus_tag="WJL20_001262"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ7683418.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(3014..3679)
                     /gene="pgmB"
                     /locus_tag="WJL20_001263"
     CDS             complement(3014..3679)
                     /gene="pgmB"
                     /locus_tag="WJL20_001263"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ7683419.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRHKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(3692..5950)
                     /locus_tag="WJL20_001264"
     CDS             complement(3692..5950)
                     /locus_tag="WJL20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ7683420.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVICWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
     gene            complement(6184..7203)
                     /locus_tag="WJL20_001265"
     CDS             complement(6184..7203)
                     /locus_tag="WJL20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ7683421.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTSYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYG
                     IESLS"
     gene            complement(7207..8562)
                     /locus_tag="WJL20_001266"
     CDS             complement(7207..8562)
                     /locus_tag="WJL20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ7683422.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
BASE COUNT         1904 a         2454 c         2440 g         1798 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaagttgag cataatataa aacagtttga aaagcagacg
      121 gcaaaccttc atgccgtctg aaaaccagct acaaggaaac atcatgcaaa ccgccgcccg
      181 ccgctcgttc gactacgata tgcccctcat ccaaacgccg acttccgcct gccaaatccg
      241 tcaggcgtgg gcgaaggttg ccgatacgcc cgaccacgag acggcaggcc gtctgaaaga
      301 cgaaatcaag gctttgctga aggagaaaaa cgcggtcttg gtggcgcatt attacgtcga
      361 tccgctgatt caggatttgg ctttggaaac gggcggatgc gtgggcgatt cgctggaaat
      421 ggcgcgcttc ggtgcggaac acgaagccgg tacgctggtg gtggcgggtg tgcgcttcat
      481 gggcgagagc gcgaaaatcc tctgccctga aaaaacagtg ctgatgcctg atttggaggc
      541 ggaatgttct ttggatttgg gctgtccgga agaagcgttt tcagcgtttt gcgaccaaca
      601 ccccgaccgc acggtcgtag tgtacgccaa cacttccgcc gccgtgaaag cgcgtgccga
      661 ttgggtggta acgtcttcgg tggcgttgga aatcgtatcg tatttgaaat cgcgcggcga
      721 gaagctgatt tggggacctg accgccacct cggcgactac atccgccgcg aaacgggcgc
      781 ggatatgctg ctgtggcagg gttcgtgcat cgtccacaac gaattcaaag ggcaggagct
      841 ggcggcgttg aaggcggaac accccgaagc ggtggtgctg gttcatcccg aatcgccaca
      901 aagcgtcatc gaactgggcg acgtggtcgg ctcgaccagc aaactgctca aagccgccgt
      961 atcgcaccct gaaaaaaaat tcatcgtagc gaccgacttg ggcatcctgc acgaaatgca
     1021 aaagcaggca cccgacaaag aatttatcgc cgcaccgacg gcgggcaacg gtggaagctg
     1081 taaaagctgc gcgttctgcc cttggatggc gatgaattcg ctgggcggca tcaaatacgc
     1141 cctgacaagc ggacacaacg aaatcctgtt ggacaggaag ctgggcgaag ccgccaaact
     1201 gcctttgcag cgtatgctcg acttcgcggc aggactcaaa cgcggggatg tgttcaacgg
     1261 catgggcccc gcctgatttg ccgtcaatat tgccgtttca gacgacctct caaacaagga
     1321 caacaccatg caaaccgatt gcgacgtatt gattgccgga aacgggctgg cggcactgac
     1381 gctcgccctg tcgctgcctg aatcgttccg catcgttatt ttgtgcaaaa accggctgga
     1441 cgacaccgcc agccgtcatg cgcaaggcgg gattgcggcg gcgtggtcgg gagaggacga
     1501 catcgaaaaa cacgttgccg atactttgga agcgggcgcg ggtttgtgtg atgaagccgc
     1561 cgtccgcgcc atcctgtcgc agggcaaacc ggcaatcgaa tggctgctgg cgcagggcgt
     1621 ggcgttcgac cggaatcata acggcctgca cctgacgcgt gaaggcgggc atacctgccg
     1681 acgaatcgcc cacgtcgccg actacacggg cgaagccgtc atgcagagcc tgattgccca
     1741 aatacgccgc cgcccgaaca tccgcgtttg cgagcggcag atggcgttgg acattcaaac
     1801 cgaatcaggc gcggcgtgcg gactgaccgt cctcgactgc cgaacacaag aaacctaccg
     1861 catccgcgcc cgccataccg tactcgcagg cggcggcttg ggacagattt acgccgccac
     1921 caccacgccg cccgaatgca cgggcgacgc catcgccatg gcgatacgcg caggctgcgc
     1981 agttggaaac ctcgaattta tccaattcca ccccacaggc ttggcaagac cgtctgaaaa
     2041 cggacgcacc ttcctcattt ccgaagccgt gcgcggcgaa ggcggcatcc tgaccaacca
     2101 agcgggcgaa cgatttatgc cgcattacga ccgccgcgcc gaactcgcac cgcgcgacat
     2161 cgttgcccgc gccatcgcag ccgaaatcgc caaacaaacg caagacttcg tctcgctcga
     2221 catcagccat caacccgcag cgttcgtccg ccggcatttc ccatccatcc atcgacactg
     2281 tttatcccaa tgcggcctag acatcacgcg ccaagccatc cccgtccgcc ccgtgcaaca
     2341 ctacacctgc ggcggcatcc aaaccgaccc ctgcggcaga acctccctgc cgcagctcta
     2401 cgccttgggc gaaaccgcct gcactgggct gcacggagcc aaccgccttg ccagcaactc
     2461 cctgctcgaa tgcgtcgtga ccgcaaggct ctgcgcccaa gccatcgcag acggacaagc
     2521 attccaagcc gaaccattcc aaaggccgtc tgaaagcctc tccgccgaag caggcatctt
     2581 ttcagacgac cttcaaaaca cattcagccg ccccgtcctg caaacgttca accaacgcca
     2641 tctaggcatc ctacgcaacg ataccggcct gcgccgcgcc atcgcccaac tgcagctttg
     2701 gaagcaaaac caagccgaac cgcacaccgc gtccgaatac gaaaaccgca acctgctcga
     2761 atgcagcctc gccgtcgccc aagccgcata caggcggaga cagaacatcg gcgcgcattt
     2821 taatagtgat tgttaaggtt ggctggttag aaaaaaaatc gttcaaataa atttgaatag
     2881 catggttaag agattggcat ttagttttta gggttgtgta agttgggtta tcattctgtt
     2941 ctaaaagcag aaaggccgtc tgaaacactg ttcagacggc cttttacttc cttgacactt
     3001 tatctgacgc gttttacctg cccgactgtg cccacacgtt ttgcaggtag gcgtaggtca
     3061 gctcggcggt gccggacacc agcgcgatgt cgctgcccaa gtcttcggct ttgcccacgc
     3121 cgatgggtaa ggcgccggcg gctttgatgg cggcgacacc tgcggcagcg tcttcaatgc
     3181 cgatgcagcg gcggatgtct gcgcccacgc cctcggcggc ggcgaggaag atgtcggggg
     3241 cgggtttgga atgcgcgacg gcggcagggt cggcaatggc gtcgaagaag tgggtcagcc
     3301 ccatgcgttc gagcaggaac gggccgtttt tactggcgga tgcgagggcg atttttttgc
     3361 cgtttgcttt caatgcttcc agcagcggca aaatgccggg gtacacgtct tcgggtttga
     3421 ctgcctgaat catctcgacg tagttgtcgt ttttgtggcg ggtcagttcg gcaaactcgg
     3481 cttcgccgac ggttttaccg ccgtgcgcga ggatgcgttt gagcgaatcg tcgcgcgaca
     3541 cgcctttgag ctgctcgtta aacttgcggt cgatgccgat gcccagttct tcggcgagtt
     3601 ttttccacgc gcggtagtgg tattcggcgg tatcggtaat cacgccgtcg agatcgaaga
     3661 ggactgcggt aaacgtcatt ttgcaccctc cttatttttc caacgcggcg gcgtggctgc
     3721 cgtcgagcgt gatgtctttg ccatacacct gcaaatcgag cggctcgcct ttgagcaggg
     3781 tgaagacgac gttttctttg ccgacggcga ctttaatcag acggcctcgg tagttgatat
     3841 ggaaggcgta gcctgtccat gcgcccggca ggaacggtgc aaagctgagt ttgccgcccc
     3901 aggttttcat ttgggcgaaa ccttggacga tggcgagcca cgagccggtc atggaggtga
     3961 tgtgcaggcc gtcttcggtg tcgttgttgt agttgtccaa gtccaggcgg gcggtgcgcc
     4021 ggtacatttc cacggctttt tcttctttgc ccagttcggc ggcgagaata gagtggatac
     4081 acggcgacaa cgagctttca tgcacggtca tcggttcgta gaagtcgaag ttgcggcgtt
     4141 tttcgtcgat attgaaacgg tcgccgaaga agtagatgcc ttgcaagacg tccgcctgtt
     4201 tgatgaaggg cgaacgcagg attttgtccc acgaccattt ctggttgagc ggcaaatcgt
     4261 cgggcgaaag cgcggacact gggcggatgt ctttatcgag gaagccgtcg tgttggacga
     4321 atacgccgag ttcttcgtca tgcggacggt acatattcgc gctgatgtcc gcccattttt
     4381 ccaactcggc ggcacgcacg ttcaaatccg gacgcgggta tttcgccaag gcttcgcggg
     4441 tgtagtccaa tacccatgcg gcgagggtgt tggtgtacca gttgttgttg atgttgtttt
     4501 cgtattcgtt cggaccggtt acgccgtgaa tcatgtattt gccgttgcgt ttggagaagt
     4561 ggacgcggtc cgcccagaag cgggacactt cgaccaagac ttccaagcct tctttagcaa
     4621 gatagccctc gtcgccggtg tagttggtgt agttatagat ggcgtaagga atcgcgccgt
     4681 tgcggtggat ttcctcgaag gtgatttccc attcgttgtg gcactcgatg cccgtaaacg
     4741 ttaccatcgg atagagtgcg cccgccaagc cctgttcgcg cgcgttgtgt tgtgcctgcg
     4801 gcagttggtt gcggcggtat tgcagcaggt tgcgggtaac ttcgggttcc gccagtgcga
     4861 ggtagagcgg cacggcgtag gcttcggtgt cccaataggt cgcgccgccg tatttttctc
     4921 cggtaaagcc tttcgggccg atgttcagac gcgcgtcttc gccgtagtag gtggagaaca
     4981 gttggaacag attgaaacgg atgccttgct gcgcttcgtc gttgccttcg atgaccacgt
     5041 cggcgatttc ccaacggtgc agccagcctg ctttgtgcgc gtccagcaag gtttcaaacg
     5101 caacgcctgc gactttttcc gacaaggcgc ggcctgcggc tttcactgct tccaagccct
     5161 gataatcgcg gctggtggta acaataaccc gtttttcaaa ggttttgggc gtgccgccga
     5221 cttcgacctc gaaagaattg gagacttgcc agtcggtttg gctgccgctg agggctttga
     5281 agctgccggc gaaagtttgc tcggcgttga cgataaattg ttccacgccg aaagggttgg
     5341 caacggtttg ggcggcaatg taggagagac tgtctgaaac gcctttgtcc aatacctgcc
     5401 agaatttttc ttcgtagttg gagtcttcgt ttttcacgtc ggcatcgatg atggaatcga
     5461 tgcggacttg gtgggttttg ccgtcaacgg atacggcttc ccaacagata accgccagct
     5521 ctttttgcgc gacggacagg aatttgcaca cattgaaacg cacaccgaat acggtgaacg
     5581 agcggcgcaa cacgccgtgc tgcatatcga gttcgacgga gaagccggca acatcgtttt
     5641 tcgccaagtc cacctcttga ccatcgacaa agattttgac tttgctgaaa ttgagcgcgt
     5701 tgatggcttt gccgaaatat ttgggatagc cgtttttcca ccagccgacg cgggttttgt
     5761 cgggaaacca aacgccggcg atgtaggtgc ccaagtggct gtcggcggaa taggtttcct
     5821 caaagctgcc gcgcataccc atatagccgt tgcccaaact ggtcaggctc tcttgcagcc
     5881 gtttgtgttc tttttccagc tttgccgaac gcagcgtcca agggctgatt tccataattc
     5941 ttgtgtacat ttatgaagct cctgtttgga ttgatttgag ggaatggtga aatcttatag
     6001 tggattaaca aaaatcagga caaggcgacg aagccgcaaa cagtacaaat agtacggaac
     6061 cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa gccgaggcaa
     6121 cgccgtaccg gtttttgtta atccactata aaaaggccgt ctgcaaccgg tgttaggaag
     6181 ctcctaagaa agggattcga tgccgtaagc aatcgtcgcc tccctagtat cacccttgtt
     6241 cagacgaata ttgccgaact cgggccaatt caggctgtcg ggcagcgtct gcgcctcggt
     6301 cgccagcgcg tcgtaaacgc ccgcatcgtg ccgcgcgaaa tcctgcgggg cggcggtaaa
     6361 gatgaccaag ccgttgcggt cgctgtatat gctgatacga cggcggcgtc cggcttgcaa
     6421 cacagcggcg ggacggccta tatcggacgg cacgcggtaa gcgtcgtcaa aaccggcccg
     6481 acccgtttcg cggcgcaggg cggcaacggc ggcatccagc ggcttgggcc ggctgaaatc
     6541 aaatacttcg aggtcgtctg aaaccgttga gacgggcagt ttttcggcat cggccggaat
     6601 atgtccgccc tgcggaatat gcagaaccgc atcgtgcagg cccgcgtcca gccgccagta
     6661 aatgtgcagc gtcgggtcga acaccgtgtc gccgagcgcg gtggcgcgat agctaacggt
     6721 aagccggtcg tcctcgtcca agcggtagga aatatccaaa tccaaatcgt tgggataacc
     6781 gtcggccgac tgttgcaggc ggctgcgcag caccaccgaa cggccgtctg ccgccaccgc
     6841 gttgaaacgg gtaacggcca gcccgtgcga accgccgtgc agcgcgttcc tgccttcgtt
     6901 ggcctccacg cggtaagtcc tgccgttgat gtcgaacgcc gcaccgcgga tgcgtccggc
     6961 cacgcgccct atctgcttgt taatctgaaa cggattgtcc gcataggaag ccgcatcgtc
     7021 gaacgacacc acgaggtttt cgcgcacgcc gtctgccaaa acggaaaatt cctgcacaat
     7081 cccgcccaag tccagcacgc agacacgcgt accacgccga ttggacagca catagctggt
     7141 tacggcacgc ccgtcgatca ggccgaaatc gcgggtagcg ggggtagcgg gggtatcgct
     7201 catcgctcaa accccgccgt gtgtttcttt aatcaggaac acggaaaacg cgcccagcag
     7261 caggacgacg ccccctacca agaacatagt ggcctgcaag ccgcccagca tagggaaaag
     7321 cacgaaactc aacagcgaag cgacgatttg aggcatacag atagagccgt taaacaggcc
     7381 caagtaagtg cccatatgct tgcccgacaa ggcgttggtc acaatcgtca gcggataagt
     7441 gataatgccc gcccaagcga tgccgattaa ggtataagac aacaccagcg cgtattggtt
     7501 gccgatgaag aaaacggaga aaaagccgag cgcgcccaaa gccaaacagc cgaaataacc
     7561 cgccttatgg tatttattcg gcactttcgc caatacaaac gaacaaatca ccgccgcaac
     7621 cgactgcacc gccgccaaaa cgccgtacca gttgcccgct tcttggtaac ctacggaaga
     7681 cgcatcggtg gtgtgccaga cgttttccgc aatcgcgcct gccgagtaag tccacatata
     7741 ttggaaggcg aaccagcaga agaattgcac caaagtaacc gtccaaaacg ccttaggcgc
     7801 ggttttcaag agttcgatcc agttggcttt ttcctgattc gcggcgacat cgatgccgtg
     7861 gtaacgggcg taggtttccg gatcgtattc cttcactttg aaaatcgtga acgcgctggt
     7921 aatcaccagc aacgccgcac ccacataaaa cgccacgacc acggtctgcg gcacaacgcc
     7981 tttctcggcg gtgttcgcca aaccgatata cgcaaacaca aacggcagaa tcgccgccac
     8041 gaccgcgccc gtatttgcta agaaactttg aatcccgtag gcgtagcctt tctgctcctc
     8101 gttgaccatg tcgccgacca tcatcttaaa cggctgcatc gccatatttg acgacacgtc
     8161 caacagcgca atcatcagcg cgccgaacga caaagccgcc agcgacgcat agccgaaacc
     8221 gaagctgccc gagttcggca tcaaaatcat cacaataacc gcaatcagcg tgccataaag
     8281 cagatacggc agacggcggc cgcccaaacg cggcttccaa gtgcggtcgg agtaatggcc
     8341 gacaatcggc tgcaccagca tccccgccag cggcggcagg atgaaaaacc agcccaaatt
     8401 gtgcgggtct gcgcctagcg tttgaaaaat gcggctcatt tgcgagcttt gcagggtaaa
     8461 ggccgtctga acgccgagaa agccgaaact gagcatccaa atcgtgcttt ttgccagcgc
     8521 gggcaaacct tgttttgctg tttgaggcgt atattccgac ataaggtaaa tccttttttg
     8581 atttgaaaag tatagt
//
LOCUS       DAWKQJ010000076         8577 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000076 DAWKQJ010000000
VERSION     DAWKQJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.076"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(111..938)
                     /gene="fghA"
                     /locus_tag="WJL20_001267"
     CDS             complement(111..938)
                     /gene="fghA"
                     /locus_tag="WJL20_001267"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ7683423.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(947..2056)
                     /locus_tag="WJL20_001268"
     CDS             complement(947..2056)
                     /locus_tag="WJL20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ7683424.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            2204..2611
                     /locus_tag="WJL20_001269"
     CDS             2204..2611
                     /locus_tag="WJL20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ7683425.1"
                     /translation="MAYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2811..3125)
                     /locus_tag="WJL20_001270"
     CDS             complement(2811..3125)
                     /locus_tag="WJL20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ7683426.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WJL20_001271"
     CDS             complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WJL20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ7683427.1"
                     /translation="MSMENFAQLLKESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(4977..5633)
                     /locus_tag="WJL20_001272"
     CDS             complement(4977..5633)
                     /locus_tag="WJL20_001272"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ7683428.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(5877..6922)
                     /locus_tag="WJL20_001273"
                     /pseudo
     CDS             complement(5877..6922)
                     /locus_tag="WJL20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(7059..7307)
                     /locus_tag="WJL20_001274"
     CDS             complement(7059..7307)
                     /locus_tag="WJL20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7683429.1"
                     /translation="MKRHPTLQPLSRQHHLGLVIANKAKSVTDDDKLTQHQAFILTVA
                     GATVGFGATWRFPYLVGENGGARMCFYSVSRCWLSASR"
     gene            complement(7401..8564)
                     /locus_tag="WJL20_001275"
     CDS             complement(7401..8564)
                     /locus_tag="WJL20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7683430.1"
                     /translation="MQRKFDLAQKPVLLSYGIDYEQEKNRQGATGYDLTTFMQSNGLA
                     FKDTGNRYTYGPDTTLKTYGIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESL
                     LADLLNQNKVPYQAGTVNSGKVKHHKTLFNLGANYSLTPNHSIFANFGQGYSLPDLQR
                     VLRDVGTGFAVNSDNVEPISVNSYDAGWVGVFGGSRAKLTGFYNTSDKVTQFLKDYSV
                     VVADTDERIYGAEFELNHKINDNWAVGGSIAYTRGQYKDASGQYRELGAFRISPTKAT
                     AFAQYTTPSEHKLRLQLLAIDGTQRAYKDSLIAKTNQKVRKTPEARISGYVVADLLGE
                     IKLPKGQLNFGIYNLTNKKYKSVFSQAAKVTYGELSSLSAQGRSYGISYTLNY"
BASE COUNT         2152 a         2051 c         2031 g         2343 t
ORIGIN      
        1 ccgtagagtc ggctccttcc ggctcagacg gcatacgttt atgccggcac ttcttaccgc
       61 cctcgtgccg gctatcccaa aactgctttg aacactgaac gctctttggt ttacttcaaa
      121 aacgcggcgt gataagcaat atgctcgcca ataaaactgg cgatgaagta atagctgtga
      181 tcgtagcctt tatggaaacg cacatcgacc ggctggtttg ccgcacggca ggtttcgata
      241 aaatcttcgg tacgcaattg tgtcggcaaa aactcatctt ccaagccttg atcgatgcgc
      301 ataccttgca ctttatagcc ttgttgaatg agtgagttcg catcatattg ctgccatttt
      361 tcacggtctt tccctaaata agcagtaaag gctttttctc cccacggcac gaggcttggc
      421 gataaaatag gcgaaaaggc agaaacactt tgataacgtt cctgattccg cagcgccaat
      481 accaatgcgc cgtgtccgcc cattgaatgc cccataatgg aacgtttgcc gttggtagga
      541 aagtgtttct caatcagacg gggcagctcg ttcaaaatgt aatcatacat ttgataattc
      601 gccgcccaag gctgttcggt cgcattcaaa taaaagcctg caccctgtcc taaatcgtaa
      661 gcatcatcgt tcggcacttg ctctccgcga gggctggtat cggaggccac cacaattact
      721 tgatgttctg ccgcataacg ctgaaagcct gatttggtaa tgaaattttg ttccgtacac
      781 gtcaagccgg aaagccaata aatcacacca agcggtcgat tttctggatt atttggcaaa
      841 tagacggcaa atttcatttc gcattgcagc gtttgggcat gatgcgccca aacttgttgc
      901 gaaccgccaa aaatttgatg ttgttcaatc agtttcatcg cataccttag tagtgaataa
      961 cggcgcggat tgatttacct tcgtgcatta actcaaaggc ttcattgatt tgatcgagtg
     1021 tcattgtgtg ggttacaaac ggttctaact caatgtcgcc tttcattgaa tcttccacca
     1081 ttttcggcag ttcagagcga cctttcacac cgccaaatgc tgaacctttc caaacacgac
     1141 ctgttaccaa ctggaacgga cgcgttgaaa tttcttgtcc tgcacctgct acgccgatga
     1201 taatggattg tccccaacca cggtgcgcac tttctaatgc ctgacgcatt acgtttacgt
     1261 tgccgataca ttcaaaggtg tgatcaatgc cccatttatt gatgtctaac aacacatctt
     1321 taatcggttt atcgtaatcg ttcgggttca aacaatccgt tgcaccgaac tgttttgcca
     1381 actcaaattt tgatggattg gtatcaatgg cgataatgcg gccggctttg gcttgacgcg
     1441 caccttgcac caccgccaaa ccaatcgccc ccaaaccaaa cacggcaaca gagtcgcctt
     1501 cttgcacttt tgccgtatta tgtaccgcac caatacctgt ggtaacgccg cagccgagca
     1561 aacatacttg ttcatggttg gcttcagggt tgattttcgc cagtgaaact tcggcaacaa
     1621 cggagtattc actgaaagtc gaacagccca tatagtgata aatcggctga ccttgataag
     1681 aaaaacgcgt cgtgccgtcc ggcattaagc ctttaccttg tgtatcacgc actgagacgc
     1741 acaagttggt tttacctgaa caacaaaact cacattcgcc acattcggcg gtgtaaagcg
     1801 gaatcacgtg atcacccggt tttacgcttg acacaccttc gcccacagca acgaccacac
     1861 ccgcaccttc gtgtccaagc accacaggga atacgccttc aggatcgctt cctgataacg
     1921 taaacgcatc agtatggcac acgccagtgt gggtattgcg gattaacacc tcgcctttac
     1981 gcggcatttc tacgtcgatt tccacaattt gtaagggttg gttaggggcg aatgccaccg
     2041 ccgcacgaga tttgatggtt gaatcggttt gtttcatttc cattgtcttt cctcttaatt
     2101 cattgacaaa gttgccttac ctgagcaaca tagcaacagt ttacacttta gagttaactc
     2161 taaagcaagt gttttttact gtttttgttt agaaggaggg aaaatggctt ataccactgc
     2221 caaagccgcc gaaaaaatag gcatctccgc ccacacccta cgtttttacg acaaagaagg
     2281 cttgttgcct aatatcggac gtgatgaata cggtaaccgc tgttttaccg ataacgattt
     2341 gcaatggttg ggcttattgc aatgcttgaa aaatacgggg atgagcttaa aagacatcaa
     2401 acgctttgcg gaatgtaccg tcattggcga cgataccatt gaagaacgcc tttccttgtt
     2461 tgaaaatcaa atagaaaatg tgaagtgtca aattgccgaa ttaaaacgct atttagattt
     2521 gcttgaatac aaattggcgt tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt
     2581 aaatttgccg caaattcctg aaacggctta gttttagtgc aataagcagg atttttgtcc
     2641 tgttcgtaat agaaatcaaa aatttgttgt ttaaaaagcg gattgtaagc aatgataaaa
     2701 aaatccacgc tctaatccgt tgcttgcttg tcttaaggta ttgaagcagc ggatgaagtt
     2761 aaaccacaga aaaaaaacgc cgcttggaag cggcgttttg atgctaaggt ttaattggca
     2821 ttttctttta aagccaaatc gacccgctcg cgcaattctt taccgggctt gaagtggggt
     2881 acatgttttt caggtacttc cacacgctcg ccggttttgg gattgcgacc gatgcgggca
     2941 ggacgatggt tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg acctcgggca
     3001 agcgatctag tcatggtgtc aaccaagact tttacgctgt actctacgtc ttttgccaga
     3061 agatgcgtgc cgtttttggc ggcaaacact tctgccaaac gaaccattaa ctcagacttt
     3121 gtcatgtctg caaccttatt cttgttcgcc ggagagtttg gctttcagca ggtcgcccaa
     3181 gctggtggtg ccggcattcg cattggcggc ggcattgacg gagttcagtg cttcgcggct
     3241 ttctttggca tctttggctt taacggaaag tttgatgctg cggtttttgc ggtcaacggt
     3301 aacgatgacg gcttcaactt cgtcgccttc tttcagtttg gtggtcaaat cttcaacgcg
     3361 gtcggctgca aattcggaag caggcaggta gccttctact tcgtcagaca gggcgataac
     3421 agcacctttg gcgtcaacag atttcacgga acctttaacc aaagaacctt tgtcgttcac
     3481 gctgatgaag ttgccgaacg gatcgccttc cagttgtttg atacccaagg agatgcgttc
     3541 tttttccacg tcgattgcca atacgacggc ttcgacttct tcgccttttt tgtatttgcg
     3601 tacggcttct tcgccggatt cggtccagga caggtcggac aggtgaacca aaccgtcgat
     3661 gccgccaggc agaccgacga atacgccgaa atcggtaatg gatttaaccg cgccggagat
     3721 tttgtcgcct ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt ggcattgttt
     3781 catacccaaa gagatacgcc ggcggctttc atcgatttcg aggatcatta cttcgacttc
     3841 gtcgcccaat tgtacaactt tgctcgggtg tacatttttg ttggtccaat ccatttcgga
     3901 aacgtgcacc aagccttcga tgccttgttc gatttcaacg aacgcaccgt agtcggtcag
     3961 gttggatact ttaccgaaca ggcgggtacc ttgcgggtaa cgacgggtca ggccgctcca
     4021 aggatcttcg cccagttgtt tcatacccag agaaacgcgt tgtttgtctt ggtcgaattt
     4081 caggacttta gcttctactt cttgaccgac ttccagaact tcgctcgggt gtttcacacg
     4141 gcgccatgcc aagtcggtga tgtgcagcag accatcgatg ccgcccaagt caacgaatgc
     4201 accgtaatcg gtaatgtttt taacgatgcc tttgatgacg gagccttctt gcaggttttc
     4261 cagcagggct ttgcgttctt cacccaaggt agcttccaga acggcgcggc gggacacaac
     4321 aacgttgttg cgttttttgt ccagtttgat cactttgaat tcaatttctt taccttcgaa
     4381 gtgagaagtg tcttttacag gacgtacgtc gaccaaagaa cccggcagga atgcgcggat
     4441 gctgctaatc ataacggtca ggccgccttt gactttgccg ttgatgatgc cggacaggat
     4501 gttgccgttt tccatggctt cttccagggc aatccaatcg gcagcgcgtt tggctttttc
     4561 gcgggacagt ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa cggtaacgaa
     4621 gtcgccgact ttaacttcaa tttcgccttg agcgtttttg aattcagcta catcaatcag
     4681 ggattctgat ttcagacctg cgtttacggt aacgaagttt tggtcgattg ccactacttc
     4741 agcggtaatc acctcacccg ggttcatttc ttgcagggta aagctttctt tcaacagctg
     4801 agcaaaattt tccatagaca tatataactc ttttcggtac accgccaagg ggtgcggggt
     4861 gggttggtgg atatctgccg tccttggcag ggcagacggg tacataaggt ttcagacggc
     4921 atatggggga tttttatgcc gtctgaaacg taatttgtgc gattatacct gaaaatttaa
     4981 acttcacgat accaatcaag cacttttttt acagtttctt ctatagtcag gcggcttgtg
     5041 tccaaaagca gggcatcggg ctgttgtttc aggggggcaa ctttgcggtt tcggtctgcc
     5101 tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg ccaaaccttc gcaggggatg
     5161 ccgatttgtt tggcgcggcg ttcggcacgg attttggatt ctgccgtcag gaagatttta
     5221 agttcggctt gggggaagac gaccgatccg gtgtcccgtc cgtcggcaac cagtcctttt
     5281 tcggtcagaa aatcgcgttg gcgttgcagc agggcggcgc ggactttagg caactgtgcg
     5341 actgcggatg cgcccatgcc gatggcttct gtccggatgc cgtctgaaac gtcttcgccg
     5401 ccgagcagga tgcggctgcc tgaaaatacg gcgggcagtt tttttgccag ttcggaaacg
     5461 ttttcttcat cgtgccatcc cacgccttgt ttttgtgcat atagggcagt caggcggtag
     5521 agtgcgccgg tatcgagata atcgtatccc aatgcggcgg caacgcgggc ggcaaccgtg
     5581 cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt tttgtctgtt cataaggggg
     5641 attcctgatg gtttggggta tgggttttgc cgtctgaagg atgtgtttcc cgttggggcg
     5701 gattctacct gttttaaagg acgattgtct aagcagacgg aacgccgcct gcccgaaaac
     5761 atccgacaga agccggcaag ccggttcgga ttccggcgtt gcgggtattg tggcgggcat
     5821 caaggcaatg ccgtctgaaa gaggtatggc cttcagacgg catcgattgc tgcagattag
     5881 aacaggttgc tgacaaaaat cagcaaaatc accaacggca caagatattt cacataagca
     5941 aaccaaatat tgaccgtcgt atggttgcct ttgtaaagca attcttcctt cgcttcgtcc
     6001 ttcatcacaa aaccgacaaa cagcgcgaaa ccgagcgcgg tcagcataaa caagatgttg
     6061 ccgctgatgt agtcgaaggc atcgaaaata tttttgccga acacggaacg tctttccacg
     6121 aaccatagct cagaatggac gggatgttgc cgaaaatgaa gatggcggct aatacaatcg
     6181 taatcgcggc ggtacggcgg attttggttt tttcctgaat ggtcgtaatc aacacttcat
     6241 aaatggtcag cgaagtcgtc aatgcggcaa tcaggagcag cgagaagaaa atcacggcga
     6301 acacagatcc tgcccacata tgcgagaaca caatcggcaa actttggaat accaaagtcg
     6361 ggccggaatc gggggcaacg ccgaagctga agagcgacgg gaaaatcata aagccggcca
     6421 gtacggcgat gatggtattg gtaatggccg tgatgaccgc ggtttgcacc agattttcgt
     6481 tcttatccag atagctggac aaggtaatca tcacgccgaa acccaagctc aaggtaaaga
     6541 acacttgtcc taaaacgaag acgaacagtt cggctgtaat tttgctgaag tcaggtttca
     6601 gatagaaggt aatgccttcc attgcgcccg gcagggtaac gttgcgcaca accatggcaa
     6661 tcaggaataa aaacagcaaa ggcatcaggt attttgctgc tttttcaata ccgccgatca
     6721 cgccttttac caagatccat tggttcacaa ccacaaatag gaaggtgtag aaagcaattt
     6781 cccaaggact gttttcgatg tgttcggtaa agaaactttt ggtgacttcg ctggaaacag
     6841 gactggaaat gtccaaatta ccgccgatga tattaacaat gtagctgata acccagccgc
     6901 cgagcaccat gtaataagcc atgatgccga acgcgccgag cagcccatcc agccgaccag
     6961 tttccaaatt ttggcaatgg gtttgccgtt catcgggccg ccgaacgcat ccagcgcgtt
     7021 cacgcctttg cgccgtccga tgacattttc caccaaaatc atcgggatgc cgataaccag
     7081 catcgcgata cagaataaaa acacatacgc gcaccaccgt tttcaccgac caaatacggg
     7141 aaacgccacg tcgcgccgaa accgacagtc gcgccggcaa cggtcaggat aaaggcttga
     7201 tgttgggtaa gtttgtcatc atcggtgacg gatttggctt tgttggcgat gaccaagccc
     7261 aaatggtgct ggcgagatag gggctgtaag gtggggtgtc gtttcatggg gggtcctgtt
     7321 ggtaaattaa attttattat aaaataaaaa aaccacaaac actagggcga tgtttgtggt
     7381 attgtggcac ataatgctga tcaataattt aaggtataac ttatcccata acttctcccc
     7441 tgtgcagaca agctggataa ttcaccataa gtcactttgg cagcttgact aaatacactt
     7501 ttatattttt tatttgtcaa gttataaatc ccaaagttta actgaccttt aggtagtttg
     7561 atctcaccca ataaatccgc cacgacatag cctgaaattc tagcctccgg ggttttgcgg
     7621 accttttgat tggttttggc aatcaggctg tccttgtaag ctctttgtgt accatcaatg
     7681 gcaagtagtt gtaagcgtaa cttatgttca cttggtgtgg tgtattgagc aaaagcggtg
     7741 gctttggtag ggctgatacg aaacgcccca agttctcgat attgaccgct tgcatctttg
     7801 tattgacctc gtgtataggc aatgctacca ccgacagccc agttatcatt aatcttgtga
     7861 tttaattcaa actccgcccc ataaatacgc tcatcagtat cagcaaccac cacgctataa
     7921 tcttttaaaa actgggttac tttatctgat gtattataaa aacctgtcaa cttggcacgg
     7981 ctaccgccaa acactcctac ccaccctgca tcataactat tgactgaaat tggctcaaca
     8041 ttatcagaat taaccgcaaa acccgtccca acatctctta gcaccctttg taaatcaggc
     8101 aaactatatc cttgcccaaa attggcaaaa atactgtgat ttggagttaa tgagtaattt
     8161 gctccaagat taaataaggt tttgtgatgc ttcactttgc cagaattgac agttcctgct
     8221 tgatacggca ctttattctg gtttaataaa tcggctaata gcgactcact gggtggcaca
     8281 aaaccatctg ttttactgtc tataatttga tgccgtacac ctgccgatat ttttaaatca
     8341 tctgtcaaag caacattagc attggcaaat atgccgtagg tttttaaggt ggtgtcaggt
     8401 ccataagtgt aacggttgcc agtgtcttta aatgccaagc cgttgctttg cataaaggta
     8461 gtcaaatcat agcctgtcgc cccttggcgg tttttttctt gttcataatc gataccataa
     8521 ctaagtagta caggtttttg agcaaggtca aattttcttt gcaaagccag tcttgct
//
LOCUS       DAWKQJ010000077         8459 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000077 DAWKQJ010000000
VERSION     DAWKQJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.077"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1316)
                     /locus_tag="WJL20_001276"
     CDS             complement(120..1316)
                     /locus_tag="WJL20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7683431.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            1667..1966
                     /locus_tag="WJL20_001277"
     CDS             1667..1966
                     /locus_tag="WJL20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683432.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            1966..2151
                     /gene="ccoS"
                     /locus_tag="WJL20_001278"
     CDS             1966..2151
                     /gene="ccoS"
                     /locus_tag="WJL20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ7683433.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            2141..3451
                     /locus_tag="WJL20_001279"
     CDS             2141..3451
                     /locus_tag="WJL20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7683434.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            3609..4343
                     /gene="dnaQ"
                     /locus_tag="WJL20_001280"
     CDS             3609..4343
                     /gene="dnaQ"
                     /locus_tag="WJL20_001280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ7683435.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            4340..5029
                     /locus_tag="WJL20_001281"
     CDS             4340..5029
                     /locus_tag="WJL20_001281"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ7683436.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            5061..5543
                     /locus_tag="WJL20_001282"
     CDS             5061..5543
                     /locus_tag="WJL20_001282"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ7683437.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTASEFKDADSRALLRDAYRSVQAKGWHVVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            5620..6291
                     /gene="rpiA"
                     /locus_tag="WJL20_001283"
     CDS             5620..6291
                     /gene="rpiA"
                     /locus_tag="WJL20_001283"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ7683438.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            6360..7031
                     /locus_tag="WJL20_001284"
     CDS             6360..7031
                     /locus_tag="WJL20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ7683439.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWTDSVGE
                     WLGIW"
     gene            7210..7956
                     /locus_tag="WJL20_001285"
     CDS             7210..7956
                     /locus_tag="WJL20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7683440.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         1845 a         2048 c         2516 g         2050 t
ORIGIN      
        1 gttcaggtaa gcggctgcaa ccgtcaatgt ctgaaaagag gcagtgctgt ttaagcatgg
       61 aaagccggat aaaaaaaact gcctccgttt ccggaggcag ttcgtgtgcg gataaaacct
      121 tacagaccgc tcaaacgggc agtgtcgtgg gcaatcatca gctcttcgtt ggtcggaatg
      181 accacggcaa cggctttgct gtcggcagtg gtaatcacgc cggcgttgcc gaagcgggct
      241 ttcaggttgg cttcttggtc gatgttcaga ccgaggaagc ccaagtagcc gatcacgcgt
      301 tcgcggatga tgtcggagtt ttcgccgatg ccgccggtaa agaccagtgc gtccaaaccg
      361 cctgcggcaa ccgccatact gccgatgtat ttggcaaggc ggtagataaa catatccaag
      421 gccaatttcg cgcctttatg ccccttggcg gcttcttctt caatggtgcg gcagtcgttg
      481 gacaggccgg aaatgccgag cagaccggat tttttgttca gcatttcagt gatttgggcg
      541 atggtcatat tggcgttttc ggcgagaaag ccgaatacgg aaggatcgat gtcgccgctg
      601 cgcgtaccca ttaccagccc ttccagcggg gtcaggccca tactggtgtc gcgcgattcg
      661 ccgttggcga cggcggtaat ggacgcgccg ttgcccaagt gggcaatgac catacgcagg
      721 tcttttttgt ctttgccgag gaagcgcgcg gtttcgtcgg cgacgaagcg gtagctggta
      781 ccgtgcgcgc cgtaacggcg caggccgtat ttttcataca actcctgcgg aacggcgtat
      841 ttgtaggcga cttcgggcat ggtttggtgg aaggaggtat cgaatacgac gacgttgggc
      901 aggcctttga aaatgctttg cgcggcacgc aggcccaaga ggtgggcggg gttgtgcagg
      961 ggggcgagcg ggatgcattt ttcgatgccg gcaatgactt cgtcgtcaac gaggatggat
     1021 tcgctgtaca gttcgccgcc gctgacgacg cggtggccga tggcgccgat gcggctgtcg
     1081 aggccgtggg ctttgagttc ttccatcagg gcttcgaccg cgccggtgtg gtcgggatgt
     1141 gcggacagat cgactttgtg tttttcgccg tttactttga atgtgatgta ggcatcgggc
     1201 aggttgagtt tttcggcaag gcagctgagc aggacttcgc cgctgccgtt atccaggacc
     1261 gcgcctttga gggacgagct gccgcagttc aaaaccaaga tcaatttttg ggacattttc
     1321 ttactccgga aagtttcaga cggcattgga atcggacacg gatactaacc ggattcgtgc
     1381 cgaatccgtt ttgccttcct gggcgggaaa gtagtggggc cgtctgaaaa ggttgataaa
     1441 agaagcaggc tattctagca aaaaatcttt gcaattgctt ggcttaatcg ggcgtttgcg
     1501 tgaaaatggc ggaagtcggg cattttgacg gaaaaaaggg ggatatgcgg tttgccggtg
     1561 ttttcggctg ggggagggag aatttctttg cattcttccc gtccaaactg gtacattcgc
     1621 cctgttttta gttggtgtcg tctgaaaaaa ggcgggtggg aaatttatgc attatttcag
     1681 tattcatgac caatcgggac ggcatatagg ctttttcatc ctgcttgccg acgacgaatc
     1741 ggaagcacgg ccgcaaagcg ggcgttttgc cgtcaaactt gaaggcggaa tggaaaatca
     1801 tgtcctgtcg ccgttcggtc agacggaaat cccccaatat tggcgcgtgg tcaaagaccg
     1861 tatcgagctg ttttttgacg aagctcccgt cggaactttg cgtaacgaat atctgaccgt
     1921 gtcggggcag acttttgttt tgaacgattt gacagggaat atgtgatgga aagcatgttt
     1981 atattggtgc ccatcagcat tattttggcg tttgtcatcg gctggttttt ttggtggtcg
     2041 ggcaaaaacg ggcagtttga cgacttggaa gggccggcgc accgcatcct gatggatgat
     2101 gattcgacaa gtaaactgat tgaaaaagag agggaaaacg atgtacgcta aaaaaggcgg
     2161 tttgggactg gttaaaagcc gccgtttcgc acctcttttc gctacgcagt ttctcggcgc
     2221 gttcaacgac aatgtgttca aaaccgcgct gtttgtgatg attgggtttt acggtttggg
     2281 gcaaaacggc ttcctgcctg ccggacagat gttgaacttg ggcgcgttgt tgtttatttt
     2341 gccgtatttc ctgttttcct cgctgtcggg gcagttgggt aacaaattcg acaaggccgt
     2401 tttggcgcgt tgggtcaagg tgctggaaat gatcattatg gcggtggcgg catacgggtt
     2461 ttatatccgg tctgccccgc tgcttttggc gtgtctgttt tgcatgggcg cgcaatcgac
     2521 gctgttcggg ccgctgaaat acgccatcct gcccgattat ctcgacgaca aagagttgat
     2581 gatgggcaac agcctgattg aatcgggtac gtttgtcgcc atcctgttcg gtcagatttt
     2641 gggaacggcg gtggcaggtg taccgcctta tattgtcggg atactggttt tgctggtcgc
     2701 cgtcggaggc acggtcggca gcctgtttat gccgtccgtc cccgccaagg ctgccgatac
     2761 ccaaattgag tggaatattg tccgtggcac aaaatccctg cttcgtgaaa cggtgcggta
     2821 caaacccgtc tttaccgcta ttatcggtat ttcgtggttt tggtttgtcg gcgcggtcta
     2881 tactacgcaa ctgccgacct ttacccaaat ccatttgggc ggcaacgaca atgttttcaa
     2941 cctgatgctt gccctgtttt ccatcggtat tgccgccggt tcggtactgt gtgccaagtt
     3001 cagcagggaa cggctgatgt tggcttgggt aacggttggt gcgttgggtt tgacggtttg
     3061 cggcctggtt ttggtgtggc tgacgcacgg acaccgtttt gaagggctga acggcatttt
     3121 ttggttttta tcgcaaggat gggcatatcc cgtgatggcg gtgatgacgc tgatcggctt
     3181 tttcggcgga tttttctccg ttccgctcta tacttggctg caaaccgcca gtagcgagac
     3241 tttccgcgcc cgcgccgttg ccgccaacaa tatcgttaac ggtattttta tggtttccgc
     3301 cgccgttttg agcgcggtgt tgctgttttt gtttgacagc atttccttgt tgtatctgat
     3361 tgtcgctttg ggcaatattc cgttgtcggt atttttgatt aagcgcgaaa ggcggttttt
     3421 aggcgcggcg gcaatcagga aaaaaccttg aatgccgtct gaaaaaattg tccccaaaat
     3481 acacaatccg cctgcccaat gttgtgaaca cactgtgaat aagtgggttt gtgcattgaa
     3541 atgaaaacag aaaaatatat tgcttaaaaa atagtcagca gatggaaaat ttgaagaaag
     3601 aagcagacat gattcaacgc caaatcatcc tcgataccga aaccaccggt ctgtatgccg
     3661 acggcggcga ccgcttggtc gagtttgccg gtttagaaat ggtcaaccgc caaatgaccg
     3721 acaaaaacct gcacctctat gtccaccccg agcgcgatat gcccgaagag gcggcgaggg
     3781 tgcacggtct gacgatagag gttttggaag ggaaaaacgc gccgccgttt gcagaggtcg
     3841 gcaggcagat tgccgatttc ctgcgcggtg cggaactgat tatccacaat gccaaattcg
     3901 acgtgggctt cctcaatatg gagttccgcc ggatggggct gccgactgtc gaggaactcg
     3961 gctgtaccgt taccgatacc ctggcgatgg cgcgcgaaat gttccccggg cagaaagcca
     4021 gcctggatgc cttgtgcaac cgtttttccg tcgaccgcag caagcgcgtc ctgcacggcg
     4081 cattgatcga ctgcgaactt ttgggcaaag tctatcttgc tatgacgcgg cggcagttcg
     4141 acctgatggg ggaggccgag gaggaagagc cggcagccaa acctgttgca tctgccgaaa
     4201 tgaaactcgg tgccaaactg aaagtgatta aggcggatga agccgaactg gcggcgcacg
     4261 ggcaatattt ggacggcttg ggcgaagcct gtatctggcg caaggaagcc gtgccgtcgg
     4321 aaaacggcgg ggcggacgca tgaagcgcaa aaatatcgcg ctgattcccg ccgccggcat
     4381 cggggcgcgt ttcggtgcgg acaaacctaa gcagtatgtc gaaatcggaa gcaaaaccgt
     4441 tttagaacat acgattggga tttttgaacg gcatgagggc gtcgatttga ccgtcgttgt
     4501 cgtctcgccc gaagacacgt ttgccgataa ggttcagacg gcatttccac aggttcgggt
     4561 gtggaaaaac ggcggacaga cccgcgccga aaccgtccga aacggtgtgg caaaactgtt
     4621 ggaaatcggt ttggcggcgg aaaccgacaa tattctggta cacgatgccg cgcgttgctg
     4681 cctgccgtct gaagctttga cgcggttgat agaacaggcg ggcaacgctg ccgaaggtgg
     4741 gattttggca attcccgttg ccgatacgct caagcgcgcg gacggtggga acattagtgc
     4801 aaccgtcgag cggacgagcc tttggcaggc acaaacgccg cagcttttcc gcgccgggct
     4861 gctgcaccgc gcattggctg cggaaaactt ggacggcatt accgatgaag cgtccgccgt
     4921 cgaaaaattg ggcatccgcc ctttgctggt gcagggcgac gcgcgcaatt tgaaactgac
     4981 gcagccgcag gacgcataca tcgtcaggct gctgctcgat gccgtctgaa cttcaattca
     5041 aaccatcagg aacacaaacg atgacgaaca tccgtatcgg acagggctac gatgtccacc
     5101 aactgaccga aggcagaaag ctgatacttg gcggagttga aatcccattt gaaaaagggc
     5161 tgctcggaca ctccgatgcc gatgcgctgc tgcacgccgt taccgacgcg ctgcttggcg
     5221 cggcaggttt gggcgatatc ggcagccatt tccccgatac cgcctcagag tttaaagatg
     5281 cggacagccg cgccctgctg cgcgatgcct atcggagcgt tcaagctaag gggtggcatg
     5341 tcgtcaatgt cgatacgacc gtgattgcac agaaacccaa actcgcgccg cacattccgc
     5401 aaatgcgtgc caacatcgcc gccgatttgg gcatagacat ttcgtgcgtc aacatcaaag
     5461 gtaaaaccaa cgaaaaactc ggctatctcg gacggatgga aggcatagag gcgcaggcgg
     5521 cggtattgct ggtacggatt tgaacgcggc gggcaggacg tgcaaaaaaa tgttaagatg
     5581 acggctgttt gttgatacgg tttcccaaag gacaaaaata tgacgacaca agacgaactc
     5641 aagcgcatcg ccgccgaaaa agcagtcgaa ttcgtacccg aaaacgaata catcggcatc
     5701 ggtaccggtt cgaccatcaa ctttttcatc gaagccttgg gtaagagcgg caaaaaaatc
     5761 aaaggcgcgg tatctacttc caaaaaatcc agtgaactgc ttgcccaata tgatattccc
     5821 gtcgtttccc tgaacgaagt atcggggttg gcggtctata ttgacggtgc ggatgaagtg
     5881 aaccacgccc tgcaaatgat taaaggcggc ggcggcgcac acctcaacga aaaaatcgtc
     5941 gccagcgcat ccgaaaaatt cgtctgcatt gcggatgaaa gcaaatatgt ttcacgtttg
     6001 ggcaaattcc ctctgcctgt ggaagtcgtc gaaagcgcgc gctctcttgt ttcgcgcaaa
     6061 ctgctcgcta tgggcggaca gcccgaactg cgtatcggct acaccacgtt ttacggcaac
     6121 caaatcgtcg atgtccacgg cttgaatatc gatcagccgc tgacgatgga agacgaaatc
     6181 aacaaaatca ccggcgtact cgaaaacgga atattcgccc gcgatgccgc cgatgtgttg
     6241 attttgggga cggaagaggg tgcgaaagtc atctatccct gccaagggta atcctgacgt
     6301 taagatgccg tctgaaatcc ggttcagaga tgttcagacg gcatcggcat ttgaaaaata
     6361 tgcagattaa aaaaatcatg aaatggcttc ccgtcgccct gtcgcttttg ggcgcgttgg
     6421 gttatacggg atacggcagc gaggcggtgc ggacggcggt tgccgtactc gacgtactcg
     6481 gcgcggcagg ggacgcgggt tccgacgcgc ccgctcgccg ccgagcatcg gcgaaatccg
     6541 gccaccgcta cacaggcacg gtgtccaaag tctatgacgg cgacaccctt cacgttatcg
     6601 acggcgacgg cgcgaaacac aaaatccgga tggcgtatat cgacgcgccg gagatgaaac
     6661 aggcttacgg cacgcgttcg cgcgacaacc tgcgtgcggc ggcggaaggc aggaaagtca
     6721 gcgtccgcgt gttcgacacc gaccgctacc agcgcgaagt ggcgcaggtt tctgtcggca
     6781 aaaccgattt gaacctgatg caggtgcagg acggggcggc gtggcattat aaaagttatg
     6841 ctaaagaaca gcaggataag gcggattttg ccgattatgc cgacgctcaa attcaggcgg
     6901 aaagggaacg caaaggattg tggaaagcta aaaatccgca agcgccgtgg gcgtaccgcc
     6961 gggcaggcag gagcggcggg ggcaataagg attggacgga ttccgtgggc gaatggttgg
     7021 gcatttggta aagacaccgc acggctttga acggcaaatg ccgtctgaaa accgcgcgcc
     7081 ctgcattttt gcggagacgc gcaaccgccg tctgccgcga taaaaaagtt caggcggcgg
     7141 tttttacggt aaaatggcgg attattttgc aacggcggca gttccgccgg tttttgatac
     7201 ggacggttta tggattttcg ttttgacatt atttacgaat accgctggat gtttctttac
     7261 ggcgcactga cgaccttggg gctgacggtc gtggcgacgg cgggcggttc ggtattgggt
     7321 ctgttgttgg cgttggcgcg cctgattcac ttggaaaaag ccggtgcgcc gatgcgcgtg
     7381 ctggcgtggg cgttgcgtaa ggtttcgctg ctgtatgtta cgctgttccg gggtacgccg
     7441 ctgtttgtgc agattgtgat ttgggcgtat gtgtggtttc cgtttttcgt ccatccttca
     7501 gacggcattt tggtcagcgg cgaggcggca atcgcgctgc gtcgcggata cgggccgctg
     7561 attgccggtt ctttggcact gatcgccaac tcgggggcgt atatctgtga gattttccgc
     7621 gcgggcatcc agtctataga caaaggacag atggaggcgg cgcgttcttt ggggctgacc
     7681 tatccgcagg cgatgcgcta tgtgattctg ccgcaggcat tgcgccgcat gctgccgcct
     7741 ttggcgagcg agttcatcac gctcttgaaa gacagctcgc tgctgtcggt cattgctgtg
     7801 gcggagttgg cgtatgttca gaatacgatt acgggccggt attcggttta tgaagaaccg
     7861 ctttacaccg tcgccctgat ttatctgttg atgacgactt tcttaggctg gatattcctg
     7921 cgtttggaaa aacgttacaa tccgcaacac cgctgattgg agtcatatca acaaaaagct
     7981 gcccgggtat tcatttgagg cagctttttt gtggcaaatt tatcgtggat taacaaaaac
     8041 cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcccc
     8101 gagtgaatcg gttccgtact atttgtactg tctgcggctt cgtcgcctgt tctgattttt
     8161 gttaatccac tataaattca aagagaaaaa gctatgccgt ctgaaagtct ttcagacggc
     8221 atctgtcagc ctcacgcaag agggaatccc atctgtttgg tttcaatttt ttgggtgatg
     8281 tatgagccgt cattcccgcg caggcgggaa tcctgtccgt tcggtttccg ttgtttttga
     8341 gtttcggttt tcccgataaa tttttgtggt ttccggttcc ggattcccgc tttcgcggga
     8401 acgacggcgg atggtttctg tttttttgat aacttcctgt gattttggat ttttggatt
//
LOCUS       DAWKQJ010000078         8349 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000078 DAWKQJ010000000
VERSION     DAWKQJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.078"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WJL20_001286"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WJL20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ7683441.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1672..2055)
                     /locus_tag="WJL20_001287"
     CDS             complement(1672..2055)
                     /locus_tag="WJL20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ7683442.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFVCTVGLFVAAA"
     gene            complement(2059..3600)
                     /locus_tag="WJL20_001288"
     CDS             complement(2059..3600)
                     /locus_tag="WJL20_001288"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ7683443.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTKPGELFVTDNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTSSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3890..4723
                     /gene="serB"
                     /locus_tag="WJL20_001289"
     CDS             3890..4723
                     /gene="serB"
                     /locus_tag="WJL20_001289"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ7683444.1"
                     /translation="MPHALVLQFPSAEVLPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENILKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDILMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            4787..6010
                     /locus_tag="WJL20_001290"
     CDS             4787..6010
                     /locus_tag="WJL20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7683445.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6171..7751
                     /gene="purH"
                     /locus_tag="WJL20_001291"
     CDS             6171..7751
                     /gene="purH"
                     /locus_tag="WJL20_001291"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ7683446.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1857 a         2281 c         2359 g         1852 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatctacg
      241 gttttcttcg catctccgaa acacatcacg ctgttttcgt tgaagaacag tgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccac
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcggggttg
      421 acggtgtcgt tcgcaccgat gaccaagacc acatcggttt cggggaagtc gtcgttgatt
      481 tcgtccattt ccaaaacgat gtcgtagggg actttggctt cggcgagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gtacttcggt gccgttttta
      601 cgtaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacatt gtttgcgcct ttcagcattt cggcaatatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagata atgtgccgct gtcgctgccg
      781 aaaccaccgg caattaccga gacaaacgag cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccagcgcg ccggtaacga tgagcaggtc gttggagagc
      901 atgaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctgccg tcaatgccga caaatacgag cagcaacaca
     1081 aacgatacgg caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgag tttgccgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgcttgga cagccccgcc agcgaaaaga taaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 tttcaatgcc gtctgaaaaa ttgacgttcg tgttttcaga cggcatctgt ttcaagcagc
     1681 cgcgacaaac agcccgacgg tgcagacaaa tccgcccgcc cacatcagcg agcgcaatgc
     1741 tgctttgtcg gcgatgtagc accagataaa ggcgaggcgg aacaggatga acaggccggc
     1801 aagcgtgttg acggttgctt gtccggcatt gccggttgca tgtgccgtca aaacggcggc
     1861 agcaaacggt gcaaaggctt caaaaccgtt ttgctgcgcg gcgtgggcac gggcggctgt
     1921 gccttgcgtg cgcgccagaa aatcgcgcgg attgtggttg tctttaaacc ggaatccgcc
     1981 cgcttttttg gcatacgccg cacaaaaaag cggcaatagg taggcaatca gaatacacca
     2041 ataggcgaaa gtcatggctt accctttctt aaacatattc agcatacgcc gtgttaccgc
     2101 aaagccgccg aagatgttga tgctggcaat caggatggcg atgaaggcaa gcagggtaac
     2161 gaagccgttg ccttggctga tttgcagcag cgcgccgacg acgatgatgc cggagatggc
     2221 gttggtcacg gacatcagcg gggtatgcag cgagtggctg acgttccaga cgacgtagta
     2281 gccgatgacg caggcgagga cgaacacgat gaagtggttc aaaaatgctg cgggcgcgac
     2341 cgcgccgacc cacagcacca acacggcggc gatgacggcg ggcgcgagtt tcttccacag
     2401 gggaacgggt ttcggctccg gcttggcggc aggcgcggct ttttcagacg acgtttgctg
     2461 cggctgggcg gaaacttgaa tcggcggagg cgggaaggtg atttcgccgt cgcgggtaac
     2521 ggtcatgttg cggataatca cgtcttcgaa gtccagcgtg atttcgccgt ctttgttcgg
     2581 gcttaacagc ttggtcaggt tcaccaagtt ggtggcgtaa agctgggaag actgtccggc
     2641 aaggcggttt gccatgtcgg tgtagccgat gattttcacg ccgttgtcgg ttacgaacaa
     2701 ttcgcccggt ttggtgagtt cgcagttgcc gcccgtcgcc gccgccaaat cgacgatgac
     2761 ggagccggat ttcatgcttt ccaccatttc tttggtaatc agcttgggcg cgggtttgcc
     2821 cggaatggcg gcggtagtga tgatgatgtc cacttctttt gcctgctcgg caaagagctt
     2881 catctcggcg gcgataaatt cgtcgctcat cactttggcg tagccgtcgc cgctgccgcc
     2941 cgattcttgc gggaagtcga gtttcaggaa cttgccgccc atcgattcga tttgttccgc
     3001 cacttccaag cgggtatcga acgcgcgtac cactgcgccg agcgagtttg ccgtaccgat
     3061 cgccgccaaa cccgccacac ctgcaccaat caccaaaacc tgcgcgggcg gcactttgcc
     3121 ggcggcggta atttgaccgg tgaagaaacg gccgaaggcg ttggcggctt caatcacggc
     3181 gcggtagccg ctgatgtttg ccattgaaga caaagcgtcc aaagcctgcg cgcgcgaaat
     3241 gcggggcacc atatccatcg ccagcgcgtt cactttcttg gcgcgcaagg cttcgaccaa
     3301 agcctcgttt tggcgcggcc acaggaagct gacgatggtt tgaccttcgt tcaaaagcgg
     3361 cagttcctgt tcggacggcg cgttgacctt ataaatcaaa gggcaggccc aaaccgccgc
     3421 tttgtcggca acggttgcgc ctgctgtttg gtaagcggca tcgtccaaac ttgccgccaa
     3481 acctgcaccg ctttcgacaa cggtttcaaa gcccagtttg cccaacaggg caacggtggc
     3541 gggcgtgcag gcgacacggg tttcgccgga taatgactcg cgtgggatac cgattttcat
     3601 ctctgaatcc tttttcgggt tgtttatatg tatcgtgggt taaatttaaa tcggggcagg
     3661 gcggagcaac gccgtaccgg tttaaagccg actcacttca aatgttaata tattttagat
     3721 aatcccctta taacgaattt tcatcaggct ggcaatagtt gcggcatttt cccgtgttgt
     3781 ccgacacata ctgccgtttg cgtttgaagt cattttccga ttgcagtccc gcccgccgta
     3841 acattctttg ccgtttcact atataatccg cattttttga gccgccgtta tgccgcacgc
     3901 cctcgtcctc caatttccct ccgccgaagt cctgccttcc gacttcccct cacgcctgcc
     3961 cgaacctgat tgcgccgatg aaaagcgtat gcgttttatc gttgaagaag ggttttcttt
     4021 aagcgaaaaa gacgcggcgt tgcttggcag ccgtcaaatc gaccacgccg tgttgccgga
     4081 tatggatttc ggcgaactcg gtttgattgt cagcgatatg gattcgacgc tgattaccat
     4141 cgaatgcgtc gatgaaattg cggcaggcgt gggtttaaaa gacaaagtag cggaaattac
     4201 cgagcgttcg atgcgcggcg aactcgattt cgaacagtct ttacgcagcc gcgtcgcgct
     4261 gttggcggga ttggacgaac gggttttggc ggatgtttat gaaaacattt tgaagctctc
     4321 gcccggtgcg gaatttttgt tggacgaatg caaaaggcac gatgtgaaat tcctgctggt
     4381 gtcgggcggc ttcacgtttt ttaccgaaag gctgcaacaa cgcctcggct tcgaatacca
     4441 gcacgccaat gttttggaaa ttgaaaacgg caggctgacc ggccgtctga aaggtagaat
     4501 catcgacgcg caggcaaagg cagatttatt gctcgaatac cgcagccgcc tcggattgca
     4561 gccacatcag gtgttggcga tgggcgacgg tgcgaacgat attctgatgc tcaaagaaac
     4621 gggcataggc gtggcttacc gtgccaaacc gaaagtgcgg gcagccgccg atgtctgtat
     4681 caacttcggc ggtttggagc gtgtacgcgg cctgttcagg taggcggata ggaaacggat
     4741 gtcgtccgaa aggttttcag acggcatttg aacggcagga acgacagtgg gacgcagaaa
     4801 actttggttt gccctggcag cagcaggcgt tatcggcggt ttggtcggca ttgtgctgac
     4861 ggaactgatg cacttcatac agcatacggc atacggttat ggcgcggacg gtgcgtacat
     4921 ctcgttccgc gaaggtgtgg cccgtgcttc ggaagggcgg cgtttaggcg tattgattct
     4981 gtgcggtatg ttggcaggcg gcggctggtg gttgctcaaa cgtttcggta agccgcaaat
     5041 cgaaatcaaa gccgccttga aacagccgtt gcaggggctg ccgtttctga cgacggtttt
     5101 ccatgttctg ctgcaaatca taacggtcgg actcggttca ccgctcggac gggaagtcgc
     5161 cccgcgtgaa atgactgccg cgttcgcttt tgccggcggc aggcggttgg gtttggatga
     5221 agacgaaatg cggctgctga ttgcttgcgc ttcgggtgcg ggtttggcgg ccgtgtataa
     5281 cgtgccgctc gcctccacac tttttatcct cgaagccatg ctgggcgtgt ggacgcagca
     5341 agccgtcgcc gccgcattgc tgacttccgt catcgccacc gccgtcgcgc gcatcggctt
     5401 aggcgacgtg cagcaatatc atcccgccaa ccttgccgta aacacttcat tactttggtt
     5461 ttccgccgtc atcggcccga tactgggcgc aaccgccgtc tttttccagc gcaccgccca
     5521 aaaattcccc ttcatcaagc gcgacaacat caaaatcatt cccttggcag tctgcatgtt
     5581 tgcgctcatc ggcgtgattg ccgtttggtt tcccgaaatt ttgggcaacg gcaaagcagg
     5641 caaccaactg acctttggcg gtttgaccgg ttggcaacac agccttgagc tgaccgccgt
     5701 caaatggctg gttgtcttga tggcgttggc ggcaggcgca tacggcggac tgattacgcc
     5761 gtctatgatg ctcggcagca ccatttcttt tgcagccgcc gccgcgtgga acagcgtttt
     5821 ccccgaaatg ccgtctggaa gcgcggcagt cgtcggagcg gcggtgtttc tcggcgtatc
     5881 gctcaaaatg ccgctgaccg ccattgtgtt cgtattggaa ctgacctacg cgccgctgtc
     5941 cctgctgctg cctttgtgca tcggcatggc gggggcattg ggaacatcgg gaaaaatggg
     6001 cttcaaataa atgccgacaa atgccgtctg aaaggttttc agacggcatt tctttgtaaa
     6061 ttgtcagcaa tatctgcaaa aacctactgc caaaaaacta gaaatagcgg ataataccgc
     6121 cccgaaaaag ttcccctttc cccatccaac tgtcagaaaa ggaagcccca atgtccgtca
     6181 tcaaacgcgc cctgatcagc ctgtccgaca aggcaggcgc ggtcgaattt gcccaaactc
     6241 tgcacaagct cggtgtcgaa atcctctcca ccggcggcac ggcgaaactg cttgccgatg
     6301 cgggcgttcc cgtgattgaa gtcgccgact ataccggctt ccccgaaatg ctcgacggcc
     6361 gcgtgaaaac gctgcatccg aaaatccacg gcggtatttt gggtcggcgc gatttggacg
     6421 aacacgttgc caagatggaa gaacacggta tcggcaacat cgaccttgtg tgtgtcaacc
     6481 tctacccctt cgccgccacc atcgccaaac caaactgcac actggaagac gcgattgaaa
     6541 acatcgacat cggcggcccg actatggtgc gttccgccgc gaaaaactgg aaacacgtcg
     6601 ccatcgttac cgacaccgcc gacttccccg ccattgccgc cgaactcgaa gccaacaacg
     6661 gcgcattgag cgacaaaacc cgtttcaacc tctcacgcaa agcattcagc cacaccgccc
     6721 aatacgacgg catgatttcc aactacctga cctccctttc agacgacgtt ttgagcggcc
     6781 agccccaaat cggcgaattc ccaagccagt tcaaccaaag ctggattaaa gtacaagaca
     6841 tgcgctacgg cgaaaatccg caccagcgcg ccgcgttcta ccgcgatatt caccccgccg
     6901 ccggcagcct cgccgcctac aaacagctgc aaggtaaaga attgtcttac aacaacatcg
     6961 ccgatgccga tgccgcttgg gaagccgtca aatccttcga cgcgcccgcc tgcgtgattg
     7021 tgaaacacgc caatccgtgc ggcgttgccg ttgcagccga taccttgacc gcctacaaac
     7081 tcgcctacgc caccgacacc accagcgcgt tcggcggcat catcgccttc aaccgcgaag
     7141 tggacggcga aaccgtcaaa cagattaccg acaaccagtt tatggaagtc ttgatggcgc
     7201 cgaagtttac cgccgaagcc ctcgaaatcg ccgccgccaa gaaaaacgtg cgcgtattgg
     7261 aagtgccgct tgaggcaggc gcaaaccgct tcgaactcaa acgcgtcggc ggcggactgt
     7321 tggtacaaac gcccgacatc tgccgcatca gccgcgccga tttgaaagtc gtctccaaac
     7381 gccaaccgac cgagcaggaa tggaacgatt tgctgttcgt ctggaacgtc gcaaaatacg
     7441 tcaaatccaa cgccatcgtg ttcggcaaag gcggacaaac ctacggcatc ggtgcaggcc
     7501 aaatgagccg cgtggacagc acccgcatcg ccgcccgcaa agcgcaggac gccaatttcg
     7561 acctcaacgg cgcgtgtgcc gcctccgatg ccttcttccc cttccgcgac ggcgtggatg
     7621 tgattgccga acagggcatt aaagccatca tccatccggc aggttcgatg cgcgatcaag
     7681 aagtcttcga cgcggcagac gaacacggca ttgcgatggt ggtaaccggc atccgccact
     7741 tccgccatta aggcagatga acaaggcaat gccgtctgaa gggctttcag acggcatttt
     7801 gcgctatttt gtgaaggtag ggatgacggt tcgggtattc ctgacagggt ggattttcaa
     7861 ggtgttgtat agggtgtagg aggattcgta aaaggtggga tgcagggtgt gcttcagccc
     7921 gctgcatcaa aaatttttgg agaaccggcg ggagtcggcg gttttggttt cggcggggac
     7981 ggtggaaatg ggtaacattg acggaatcga cggaagcggt gggctgaagc ccacccttgt
     8041 atattggaat cactgtatca tagcaacaaa ccgcccggcc gccacccgcg cccacccaag
     8101 gcacacaacc gttgcgtagc tcagggagcg gcagggcaac ccatcgacac aaccggacag
     8161 ttgccggaca acacaaccga atgcaaggca ggtttatgat gagtacccaa taccattacg
     8221 caggtatagt gaattaaatc taaaccagta cagcgttggt tcgccttagc tcaaagagaa
     8281 cgattctcta aggtgctgaa gcaccaagtg agtcggttcc gtactatccg tactgtctgc
     8341 ggctcgccg
//
LOCUS       DAWKQJ010000079         8286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000079 DAWKQJ010000000
VERSION     DAWKQJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.079"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            156..716
                     /locus_tag="WJL20_001292"
     CDS             156..716
                     /locus_tag="WJL20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683447.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            727..1569
                     /locus_tag="WJL20_001293"
     CDS             727..1569
                     /locus_tag="WJL20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683448.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISSI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANS
                     VQPI"
     gene            1636..2262
                     /locus_tag="WJL20_001294"
     CDS             1636..2262
                     /locus_tag="WJL20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7683449.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEAIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYKTAFCLVWKILMPFFP"
     gene            complement(2469..3806)
                     /gene="ribB"
                     /locus_tag="WJL20_001295"
     CDS             complement(2469..3806)
                     /gene="ribB"
                     /locus_tag="WJL20_001295"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ7683450.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKVGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            3915..4055
                     /locus_tag="WJL20_001296"
     CDS             3915..4055
                     /locus_tag="WJL20_001296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683451.1"
                     /translation="MPSEAGFVASDGIAATPSAGRLFLPARFRFGICRKLIPKKISDK
                     RP"
     gene            complement(4021..5032)
                     /locus_tag="WJL20_001297"
                     /pseudo
     CDS             complement(4021..5032)
                     /locus_tag="WJL20_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(5200..5793)
                     /gene="ribA"
                     /locus_tag="WJL20_001298"
     CDS             complement(5200..5793)
                     /gene="ribA"
                     /locus_tag="WJL20_001298"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ7683452.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(5786..6460)
                     /locus_tag="WJL20_001299"
     CDS             complement(5786..6460)
                     /locus_tag="WJL20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683453.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            6610..6694
                     /locus_tag="WJL20_001300"
     tRNA            6610..6694
                     /locus_tag="WJL20_001300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6644..6646,aa:Leu,seq:tag)
     gene            7214..8248
                     /gene="purM"
                     /locus_tag="WJL20_001301"
     CDS             7214..8248
                     /gene="purM"
                     /locus_tag="WJL20_001301"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ7683454.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         2233 a         1835 c         1973 g         2245 t
ORIGIN      
        1 agatatttct caaaaatata atgaaatagc aaatactttc tttgttaaag ttcgaaataa
       61 tctaaaacaa aaccaccacc tgacccaact acgcgatttc ctactaccca tgctgatgaa
      121 cgggcaggta tccgtaagat gtagcggagc aagagatgga taaactattt aaaatattat
      181 taaatataag tagtacatct ttgctgttat caatatattt tattaaatcg caatattata
      241 tattagacct tacttggttt tatcgtgtaa tttcaaatca ttggatagaa cctatttcta
      301 agttaagtct acttttttat tttctaatac catttatcgc aaccgctact gtcttatggc
      361 taagcaaata cttggggaag gatgaattca agcaaggaga ggtgaaagaa ttagaatatg
      421 ttaacgataa tttcttacct agctatcttg gttatttttt tgttgctcta agcatacccg
      481 ataataatct ttttttgcta tttgttatgt atggaattat tttcttacta gtttcatgct
      541 ctaagtcatt ttattttaac cctgtttttt tcttatttgg ctatcgattt tatcaagcaa
      601 aaactgaatc agggttatta ttagtgctga ttagtaaaca ggaatttaga acgccacaaa
      661 gtgtaagttc gattgctgta tatcgaatta ataattttac tttcttagaa aaataggagt
      721 tcaataatga attttgtttt agctaaaatt acttgcaaga aagatttagt aaaaatatta
      781 tctgatgatc atattttccc tgacttttct tacgagaatt taaattttat aacatataac
      841 tatgattaca atcttgatga tgatacttgg tttcaaattg aaaatttaaa gaatcaagat
      901 ttttgtccta agtttttgga taactcaaat ttatttgatt caaaaatgtt tagtgaaata
      961 aaaaaggaag aaataaatat agagaaacta aagtatttag tatcttgtac taatgatgca
     1021 ctattcttcc agaaaatcac aagttcatta ttactcaaaa agaagcactt gctaactata
     1081 tgcggaaatg gtgcgaaact atgcgaacca caagacttat tggttattaa agatattcct
     1141 gatgctgttt atataataaa ggatgataaa ttaatattta gaaccctttc ttctatttca
     1201 agtatattta aagggattga agatttgtat cgagaagcta caaatactga agtacagcaa
     1261 ttcttagaaa gcgattttat tgatttaaaa gaagattttt taagtgaaaa agtatctatt
     1321 cccaatagga aaagaatagc tttagttcaa gatagattga ataatatgac gctagatcaa
     1381 agacaagaac tattaaatta ccttgctgaa tataataata atatcttgaa atttaacgca
     1441 gatggctctc gtgtagaaat ttctactgat gtacagctga aacatttgtt atatggtatt
     1501 gatgaacgtt attacacaac agcattagga aaagagaaaa ggttggcaaa tagtgttcaa
     1561 cctatttaat ctgccagcaa accaactaat gctaaacaaa atttaagcga cgctagcaac
     1621 aaaccgagac attccatgac cgaacaacac tttaccgaac aaatcaaatc cctaatcgac
     1681 agcttaaaaa ccatttgcgc caactatggc ttaggtaacg atggcaacga atttaaaatc
     1741 atcagtcagg cgtttttgta taaattccta aatgacaaat acgatttcga agtgaaacaa
     1801 atccgcaaag aaaaccctga cgaggcgatt gagttcatca atatggatat tgagggaaaa
     1861 acagcggttt tgaagcctga acattccatc aagtatttgt cggagcgaca aaacggcgcg
     1921 gactttgcca aactctttga tgatacgctg accgatattg ccgcacataa tgcggaactg
     1981 ttttcggtaa aaactgaagg cggggcgaaa atcgtgttgt ttgagcgtat tagtcagtac
     2041 attactgacg aaggtcgccg cgacgatttt tgccgcgcgc tgatttctaa gttggcgggt
     2101 tttagttttg aggcgatttt tgcgcaaaaa tttgattttt tcgccacgat ttttgaatac
     2161 ctgattaaag actacaacag caattccggc ggaaaatacg ccgaatacta caagacggca
     2221 ttttgtttgg tttggaaaat acttatgccg ttctttccct gaaagagaga atccaaaaaa
     2281 ccaaagccac aggaatttat cggaaatgac aaaaacccga cgaaccggat tcccgcctgc
     2341 gcgggaatga cgaattagaa gttacccgaa atttgcaaaa acaaaatcca acccaacaga
     2401 ccgggttttc gtttgcacgg aaataatgca ataaataaag caaatataaa gtatttgaat
     2461 ttactatatt atttttccgc ttcttcaaag ccgacgactt ccaaaccgaa gccggtcagg
     2521 ccggtgaaag atgagggctg cccgaggacg cgcagttttt tgacgttgag gccggcgagg
     2581 atttgcgcgc ctatgccgta gcttttgctg tcccatttgt aggcttggtt tgcgccttta
     2641 ggcagggtgc ggtcgagcag ggatgcgccg tcttcggtgc ggtgcagaag gatgacgacg
     2701 ccgctttctg cctgttggac gcgctcgagt gctttgggca atgaccacga atggcgcggg
     2761 ttggcttgga tgaagtccat cacgctgaag ggttcgtgga cgcggacgag ggtttcggtg
     2821 tcggcagagg gcgtaccttt gacgagggcg aggtgggttt cgccggagag tttgtcgacg
     2881 taaacgtgtt gttggaactc gccccacggg gtttgtacag gcgcattgcc catgtcttcg
     2941 agcaggcttt cggtacggct gcggtattcg atgaggtcgg taatggtgcc gattttgagc
     3001 ttgtgttctt cggcgaattt catcagttcg ggcatacgcg ccatcgtgcc gtcgtcgttg
     3061 atgatttcgc aaataacggc ggcaggaatc agcccgttca tttgcgccag gtcgacgccg
     3121 gcttcggtgt gtccggcgcg gacgagtacg ccgccttttt gggcgcgaag cggaaagata
     3181 tgaccgggtt ggacgatgtc ttcgggtttg gcggtcgggg aaacggcggt ttgaatagtc
     3241 agggcgcggt cggcggcgga aatgccggtg gtaatgccgt gtgcggcttc gatggagacg
     3301 gtaaagttgg tgccgtattg cgcgccgttt ttttgggtca tcatcggcag cccgagtttt
     3361 tcgaccattt cgccgtccat cggcaggcag accaagccgc gcgcgtgttt gatcatgaag
     3421 ttgatggctt cgggcgtgac gaattgcgcc gccatcagca ggtcgccttc gttttctcgg
     3481 tcttcggcat cggtgatgat gaccattttg ccgactttga tgtcggctag gatttcgggg
     3541 atgggggaaa tatgggacat ggtgtgtttt cctgtgtgtt cgggcgggcg ggcggtgtgt
     3601 tcggtatcga gccagaaggc gggcattttt gccgcctgtt cgattttacg ggcttttttc
     3661 tcgccgaagg atttgttttt caaaagggcg gagagttcgc ctgtgttgag ggcaacggct
     3721 tcggcaaaac gggtttgcaa accgccgtag tgttctgcta tccattgccg caggttgcgg
     3781 cggcgcagac cggctgtgtc ggtcatcgtt tcatcctttg tagaaatcgg atgtagcgga
     3841 ttgtaatcgc gtatttacca aaaagcaaat ctgttttttt cgccaaaccg aattatttgc
     3901 tttttggtaa acagatgccg tctgaagccg gattcgtggc ttcagacggc attgccgcaa
     3961 ccccgtcggc ggggcggctg tttcttcccg cccgcttccg attcgggatt tgccggaagc
     4021 ttattccgaa aaaaatttct gataaacggc cttgattgcc gggtacagca taggcggtac
     4081 gagcaggcgc aatatcggca atcctatgat gaatgccgtc ttcccgataa aaaccggatg
     4141 gattgccttc gcgccgaata tctttctgca tttctcttta aatgaaaaat attctcggta
     4201 gacaacgata atgtttttgg aaaaataaag ccttaccgat atagatctca gatagccgat
     4261 attgtcgaag acaaattttt gcaaaccgcc gtagatgggg gaatttttgt tttcgactaa
     4321 aaaatccacg ccccggtcga gatgcgtcag catatcgctg aaatttttgt atttgatgtt
     4381 gtggctgata gagttgccga gaacgcggta ctggtaaaaa gggttatcgt aaaaggcaaa
     4441 agtcttgata aaacgcgcca attgcaaact gtacgggaaa tcctcgtgaa tgtatccttt
     4501 tgggaaaaac agattgtttt tgatgattat ttcccgcctg acaatctttg tccacgcgtt
     4561 ggcgatatag taccgcccct ccaccaacgt ttcaaaatgg cggacaaaat cattatccga
     4621 aaagtccgcc cctttgggga tgtcgcggta attgaaggac gagggatgca ggatcaaatc
     4681 aacctttttg tctgcaagtt gttgtaaatc aaagagaatc cccccccgcg ttttttgaac
     4741 ggttggtatc ggcccaatag tcgtcgctgt ccaaaaagat taggtaatcg ccttttgccg
     4801 cccggatacc ggcgttgcgg gcatccgaca gcccgccgtt ttcttgatga atcactttta
     4861 tatgcggata tttgcctgca tattcgtcgc aaatcttccc gcagccgtcc ggcgaaccgt
     4921 catcgaccaa aatcatttca taatcggcaa aattttcggc aagcacggaa tccacgcagc
     4981 agcggaggta tttttccaca ttgtaaatag ggacgatgat ggagaaaatc ataaatatca
     5041 atccattata ttaagatgtt tgcgcgtatg cctcaaaccc gcacccgcaa tgcgtttgca
     5101 tccgcaccct gtaactttat aaaacttatt gctttatcaa ggtatggaaa cctgtttccc
     5161 gaaaggcggc gcaggatgcc cgttccctgc atctttgcct tattccgaca tcagatgccc
     5221 cagcttgtct gctttggttt ggaggtagcg tttgttttca aggttttccc cgacgtgcag
     5281 ggggatgcgt tcgaccacgt taatccccgc atctttcagg gtttggattt tttcggggtt
     5341 gttggtcagc agtttgaccg agcggatgcc cagatattcg tagatagatt gcgccaaacg
     5401 gaaatcgcgg gcatcgacgg gcagcccgag tgccaaattg gcttcaaccg tatccatacc
     5461 ttggtcttgc agatgatagg cgcggatttt gttaatcagc ccgatgccgc gtccttcctg
     5521 acgcagatag acgatgatgc cgcgcccctc tgtctgtacc gccctcatgg ccgcttcaag
     5581 ttgaggtccg cagtcgcatt ttctcgagaa cagcgcatcg cccgtcaggc attcggagtg
     5641 gatgcgcgtc agaaccggat tgccgtctga aaaattaccg acggtcagcg cgacgtgttc
     5701 ctgcccgttt gcctcttcaa agccgtgcat cgtaaatacg ccccattcgg tcggcaggcg
     5761 gcaggatgca acatagttta ataattcaga cattttcatc tccttctgcg gcagtgatgc
     5821 ccaaaagctg cttcaaaggt tcggacaaag ccaaggcaag agccacccat tccgcctgcg
     5881 ctgcctcatc cgcacactct gcgcattcaa attccacatg aaccacgccc aacacaccgc
     5941 cactttccgt gcagattgga atggaaattt gcgccgccga agcatgattg cgttctcccg
     6001 aaagctcccc caaatccaac caacggcgta catccgaggc aacattcatc cagccgcttt
     6061 gcgcgcaacg gcaggcaagt gaaacatcgg caacattttc atctagattc catatgtttt
     6121 ccaaaccttc tccctgttgg gacaggcata tcagttggaa agcctggttt tcagacggca
     6181 tcaaggcata gaccgccgca ctccgcacgc ctgtcgagcg cgaaaacacc gaatccaaag
     6241 ccatgaaaag ccgtttcagt gcatcttcgc ggacattgtc gcacgacagg taatcggcaa
     6301 gtttccaacc ctcatcactg cgccacaaaa ccgaacggtc tatcgaagcc gtccccatac
     6361 ccattaccgt ctgcgccgtc agatacgccg cccgaacctc gtcaagcggc agcttcaaac
     6421 cctgagtcag cagaaaatcc ttaatcaaag cagcaggcat actcaaattc cgtcaataaa
     6481 ataaaaccgc cggattccga tgccgggcgg atgaatagcg gattttaccg ctgcaaagca
     6541 taagcctcaa ctattacgcc caaaaccttg cacaaaaaaa caaaatctgt ataatcgtcc
     6601 aattcccatg cggacgtggc gaaattggta gacgcaccag atttaggttc tggcgccgag
     6661 aggtgtgaga gttcgagtct ctccgtccgc accaataatg ataaataaat tatatagtta
     6721 atgcttatcg ggacaaatta gtgccacaaa acataagtcg tctgcttttc agcgggcggc
     6781 ttttttatct gcctatatgt ttcaacacgc agaacggtac ataaagtgcc gccatttgtt
     6841 ccgcaccgaa tcggaaacgg gtgcttccgc ccgcaatcag attcaccgcc cctaatgccg
     6901 gggtttcaac cggaaagaag aaaatattga ttttgatgat ggcatcaaaa aaccccttcc
     6961 gctcgtctgc caaaaggaat ggttaagaaa agcaatgcgt cttttgatgg aacgcgatat
     7021 ataaggaatt gaggggaagg gcaggcgggt ttctgcccgt taatcctgcg gacggtcccg
     7081 ccaaacccga cacgcgcggg tatatggcgg actgtttaca atattgtcta aaaatctgaa
     7141 cctgcaaacg tgaaataagg tagaatacgc cccgttattt taccgtccaa tccccattta
     7201 ccaaggaaaa acgatgagta cttcattgag ttaccgcgat gcaggtgtcg atatcgacgc
     7261 aggcgaccaa ctggtcgaaa acatcaaacc gtttgccaaa cgcaccatgc gtccggaagt
     7321 attgggggat ttgggcggtt ttggcgcatt ggtcgaaatc ggcaagaaat ataaaaatcc
     7381 cgtattggtc agcggtacgg acggcgtggg taccaagctc aaacttgcct tcgattggga
     7441 taaacacgat acggtgggca tcgaccttgt tgcaatgagt gtcaacgata ttttggttca
     7501 aggagcagaa cctctgtttt tcttggatta tttcgcctgc ggcaaattgg atgttccgcg
     7561 cgcaaccgat gtcatcaaag gcattgcaca aggttgcgaa gaatccggct gcgccctgat
     7621 tggcggggaa actgccgaaa tgccgggtat gtatcccgtc ggagaatacg atttggcggg
     7681 ttttgccgtc ggcgtagtgg aaaaagagaa tgtcattacc ggccgcagca tcggcgcggg
     7741 cgatgtggta ttgggtttgg cttccaacgg cgcacattca aacggctatt cccttatccg
     7801 taaaatcatc gaacgcgaca atcccgattt ggatgccgag tttgataatg gcaaaacctt
     7861 gcgtgaggct gttattgcgc cgacccgtct gtatgtgaaa ccgattcttg ccgctttgga
     7921 aaaatttacc attaaaggta tggcacacat taccggcggc ggcattaccg aaaacgtgcc
     7981 gcgcgtgttg cctgaaaaca cggttgcaca aatcgatgct aaatcgtggg aattgcccaa
     8041 gctcttccaa tggcttcaaa aggcgggcaa tgtggaaacc caagaaatgt accgaacctt
     8101 taactgcggc atcggcatgg tcgttattgt tgctgccgaa gatgccgatg cggttcaggg
     8161 tctcttgggt gaacaaggtg aaacagttta ccgtttaggt ttgatccgtg agcgtcaggg
     8221 agacgagcat caaacccagg ttgcctgatt gcttctatag cgaaatgccg tctcaaacat
     8281 gggttt
//
LOCUS       DAWKQJ010000080         8273 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000080 DAWKQJ010000000
VERSION     DAWKQJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.080"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            108..1277
                     /gene="prpF"
                     /locus_tag="WJL20_001302"
     CDS             108..1277
                     /gene="prpF"
                     /locus_tag="WJL20_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ7683455.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1691..2890
                     /locus_tag="WJL20_001303"
     CDS             1691..2890
                     /locus_tag="WJL20_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7683456.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2998..3618
                     /locus_tag="WJL20_001304"
     CDS             2998..3618
                     /locus_tag="WJL20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ7683457.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3745..4188)
                     /locus_tag="WJL20_001305"
     CDS             complement(3745..4188)
                     /locus_tag="WJL20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ7683458.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4362..4730
                     /locus_tag="WJL20_001306"
     CDS             4362..4730
                     /locus_tag="WJL20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7683459.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4775..5929
                     /locus_tag="WJL20_001307"
     CDS             4775..5929
                     /locus_tag="WJL20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7683460.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5986..6858)
                     /locus_tag="WJL20_001308"
     CDS             complement(5986..6858)
                     /locus_tag="WJL20_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ7683461.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDVQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6943..7755
                     /locus_tag="WJL20_001309"
     CDS             6943..7755
                     /locus_tag="WJL20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ7683462.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1782 a         2279 c         2404 g         1808 t
ORIGIN      
        1 ggcgaacccc gacagtttgc ggaatcaaac ggctttggtc ggagtggcag cctaatccac
       61 tataaaaatc gtgggcagag cccacgctac ataaggagaa tctagaaatg ccgcaaatta
      121 aaattcccgc cgtttactac cgtggcggta catcaaaagg cgtgtttttc aaacgttccg
      181 acctgcccga ggcggcgcgg gaagcgggaa gcgcacgcga caaaatcctc ttgcgcgtac
      241 tcggcagccc ggatccctac ggcaagcaga tagacggttt gggcaacgcc agctcgtcca
      301 ccagcaaggc ggtgattttg gacaagtccg aacgcgccga tcacgatgtc gattaccttt
      361 tcgggcaagt ttccatcgac aaaccttttg tcgattggag tggcaactgc ggcaacctca
      421 ccgccgccgt gggcgcattt gccatcgagc aaggcttggt cgataaaggc aagattcctt
      481 cagacggcat ctgcacggtc aaaatctggc agaaaaacat cggcaaaacc attattgccc
      541 atgtaccgat gcaaaacggc gcagttttgg aaacaggcga ttttgagctc gacggcgtaa
      601 cgttcccggc agccgaagta caaatcgaat ttcttgatcc agccgacggt gaaggcagta
      661 tgttcccaac cggcaatttg gtcgatgaaa ttgatgtgcc gaatataggc cgtttgaaag
      721 ccacgctcat caacgcgggc attccgaccg ttttcctgaa tgccgccgac ttgggctaca
      781 cgggcaaaga gttgcaagac gacatcaaca acgatgccgc ggctttggaa aaattcgaga
      841 aaatccgcgc ttacggtgcg ctgaaaatgg gtctgatcag cgacgtatcc gaagctgccg
      901 ctcgcgcgca cacgccgaaa gtcgccttcg tcgcgcccgc cgccgattac accgcctcca
      961 gtggcaaaac cgtgaatgcc gccgacatcg atttgctggt acgcgccctg agcatgggca
     1021 aattgcacca cgcgatgatg ggtaccgcct ctgttgccat tgcgaccgcc gccgccgtgc
     1081 ccggtacgct ggtcaacctt gccgcaggcg gcggaacgcg taaagaagtg cgcttcgggc
     1141 atccttccgg cacattgcgc gtcggtgcag ccgccgaatg tcaggacgga caatggacgg
     1201 ccaccaaagc ggttatgagc cgcagcgcac gcgtgatgat ggaaggttgg gtcagggtgc
     1261 cggaagattg tttttaaatt gacgtagcat gggtttgccc gcgagccata aacaggtcgt
     1321 ctgaaaaaca agtaaacatc aaatcactga ccattccttt cccttgccct gtggcggaag
     1381 gcggcaaatc acaaggaaga acacggaaac cccgataaaa gacagcttcc cgtattaccg
     1441 tcattcccgc gcaggcggga atctagacct gtcaatatgg aggattggta ggggaaaaca
     1501 ggtttcgtga gttctacatt ctggattccc gccacagcct gtcctcgcgt aggcggggac
     1561 ggaataacga tagaaaatgc ggcatacgct ttgcccaaag aggccgtctg aaacaccttg
     1621 cgcctgatgt ctgccttttt cagacgaccc cacaccaaaa aaacaaccac aaactacaag
     1681 gagaaacatc atgtccgacc aactcatcct cgttctgaac tgcggcagtt catcgctcaa
     1741 aggcgccgtt atcgaccgaa aaagcggcag cgtcgtccta agctgcctcg gcgaacgcct
     1801 gaccacgccc gaagccgtca ttacgttcaa caaagacggc aacaaacgcc aagttcccct
     1861 gagcggccga aattgccacg ccggcgcggt gggtatgctt ttgaacgaac tggaaaaaca
     1921 cggtctgcac gaccgcatca aagccatcgg ccaccgcatc gcccacggcg gcgaaaaata
     1981 cagcgagtct gttttgatcg accaggccgt aatggacgaa ctcaatgcct gcattccgct
     2041 tgcgccgctg cacaaccccg ccaacatcag cggcatcctt gccgcacagg aacatttccc
     2101 cggtctgccc aatgtcggcg tgatggatac ttcgttccac caaaccatgc cggagcgtgc
     2161 ctacacttat gccgtgccgc gcgagttgcg taaaaaatac gctttccgcc gctacggttt
     2221 ccacggcacc agtatgcgtt acgttgcccc tgaagccgca cgcatcttgg gcaaaccttt
     2281 ggaagacatc cgcatgatta ttgcccactt aggcaacggc gcatccatta ccgccatcaa
     2341 aaacggcaaa tccgtcgata ccagtatggg tttcacgccg atcgaaggtt tggtaatggg
     2401 tacacgttgc ggcgacatcg atccgggcgt atacagctat ctgacttccc acgccgggat
     2461 ggatgttgcc caagtggatg aaatgctgaa caaaaaatca ggtttgctcg gtatttccga
     2521 actttccaac gactgccgca ccctcgaaat cgccgccgac gaaggccacg aaggcgcgcg
     2581 cctcgccctc gaagtcatga cctaccgcct cgccaaatac atcgcttcga tggctgtggg
     2641 ctgcggcggc gttgacgcac tcgtgttcac cggcggtatc ggcgaaaact cgcgtaatat
     2701 ccgtgccaaa accgtttcct atcttgattt cttgggtctg cacatcgaca ccaaagccaa
     2761 tatggaaaaa cgctacggca attcgggcat tatcagcccg accgattctt ctccggctgt
     2821 tttggttgtc ccgaccaatg aagaactgat gattgcctgc gacactgccg aacttgccgg
     2881 catcttgtag ccaaaaaagg gacgagtccg caaaaatgcc gtctgaaatt ttcagacggc
     2941 attttgtttg gcgtttgggc gtacaatacg gaaaacatga cgataaggaa acaaaccatg
     3001 gcacagtttt tcgccgttca tcccgacaac ccccaagaac gcctcatcaa gcaggcggtc
     3061 gaaatcgtca ataaaggcgg cgtggtcgtt tatccgaccg attcctgtta tgccttgggc
     3121 tgcaaactcg gcgataaggc ggcgatggaa cgcatactct ccatccgcaa aatcgatttg
     3181 aaacaccacc tgaccctgat gtgcgcagat ttgagcgagt tgggcacata cgccaaagtc
     3241 gacaacgtac agtttcgtca gcttaaagcc gccacacccg ggccttatac ttttatttta
     3301 caggcgacga aggatgtgcc ggcgcgcacg ctgcacccga aacgcaaaac catcgggctg
     3361 cgtattcccg ataatgccat tgcacaagcc ctgctggggg aattgggcga gccgctttta
     3421 agctgcaccc tgatgctgcc cgaagacggc gaaccattga ccgatcctta tgaaatccgc
     3481 gagcgtttgg aacacgccgt cgatttggtg attgacggcg gctggtgcgg aaccgagccg
     3541 accaccgtcg tcgatatgac cgacggcacg gaattggtgc gccaaggttg cggcgatacg
     3601 gcggtgttcg gtttgtaggg aaaccgatgc cgtctgaagc atcggctgtt cagacggcat
     3661 tgcgcgcctt gccggcggca gtccgaaatg ccggcgcgta tcgcgctcgg tcggaatatc
     3721 cgtttgaaac ggcattttga tgcattactg caccgcaatc ggaattctcg gttcgtagag
     3781 caggtcgtag gtcggcttgt tgagcaggtc ttggagcgtg aaaccgtcca gatacgtgaa
     3841 aaacgacttc atcgcgccgc cgagtatgcc cgtcagccgg caggacggtg taatcaggca
     3901 ttcgttgttc tcgcccatgc actcgaccag ctgcatcggt tcgaggtggc ggacaaccga
     3961 gccgatgttg atgcggtcgg gcggtgcggc aagccgcaga ccgccgcctt ttccgcgcac
     4021 actgtggagg aagccgcctt tgaccagcgc ggtaacgacc ttcatcagat ggcttttgga
     4081 aatgccgtag gttacggcga tggtactgat gttgaccagc gcatcgtcgt tgatggcagt
     4141 gtagataagg acgcgcagcc cgtagtccgt atgttgtgtc aaatacatga ttttctcggt
     4201 atggattgtt attcttatcg gtacggttta aggttcacgg acaatacctt aatggttgaa
     4261 accctgtccg tcggggcggt agaatgcagc ctgtctgcgg cggtatgccg tctgaaacat
     4321 ccgcgctacc gtttgataat ttgttattgt aactcaaaat catgaaaccg ttgaaacgac
     4381 atcccgccct tatcgggctt tcgcgtgacc accaccattc gctttccctg tgcgtgcgtc
     4441 tgttgcggac gccggaagaa aggcatcggg acgaactcga accgcatttt gccgaattgg
     4501 aaacccattt tcgcgaagaa gaagccaagt ttgccccaat ttggcagaat gtcgcccccg
     4561 aattgaaaca acgtttcgag aaagaccacg cccgactgcg gcagatgatg gcaagccccg
     4621 aatacggtaa cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac cacgcgcgct
     4681 ttgaagaacg cgagctgttt cccgccgccg aaccgttttt gccggcatga ttccgttttg
     4741 cggcaaatat attaatgata aacaaggaac acacatgaaa tttaccaagc accccgtctg
     4801 ggcaatggcg ttccgcccat tttattcgct ggcggctctg tacggcgcat tgtccgtatt
     4861 gctgtggggt ttcggctaca cgggaacgca cgagctgtcc ggtttctatt ggcacgcgca
     4921 tgagatgatt tggggttatg ccggactggt cgtcatcgcc ttcctgctga ccgccgtcgc
     4981 cacttggacg gggcagccgc ccacgcgggg cggcgttctg gtcggcttga ctatcttttg
     5041 gctggctgcg cggattgccg cctttatccc gggttggggt gcgtcggcaa gcggcatact
     5101 cggtacgctg tttttctggt acggcgcggt gtgcatggct ttgcccgtta tccgttcaca
     5161 gaatcaacgc aactatgttg ccgtgttcgc gctgttcgtc ttgggcggca cgcatgcggc
     5221 gttccacgtc cagctgcaca acggcaacct aggcggactc ttgagcggat tgcagtcggg
     5281 cttggtgatg gtgtcgggtt ttatcggtct gattggtacg cggattattt cgttttttac
     5341 gtccaaacgg ttgaatgtgc cgcagattcc cagtccgaaa tgggtggcgc aggcttcgct
     5401 gtggctgccc atgctgaccg ccatgctgat ggcgcacggc gtgatgcctt ggctgtcggc
     5461 ggctttcgcg tttgcggcag gcgtgatctt taccgtgcag gtgtaccgct ggtggtataa
     5521 acccgtgttg aaagagccga tgctgtggat tctgtttgcc ggctatctgt ttaccggatt
     5581 ggggctgatt gcggtcggcg cgtcttattt caaacccgct ttcctcaatc tgggtgtgca
     5641 tctgatcggg gtcggcggta tcggcgtgct gactttgggc atgatggcgc gtaccgcgct
     5701 cggtcatacg ggcaatccga tttatccgcc gcccaaagcc gttcccgttg cgttttggct
     5761 gatgatggcg gcaaccgccg tccgtatggt tgccgtattt tcttccggca ctgcctacac
     5821 gcacagcata cgcacctctt cggttttgtt tgcactcgcg cttttggtgt atgcgtggaa
     5881 gtatattcct tggctgattc gtccgcgttc ggacggcagg cccggttgag acaaaccgcc
     5941 gcagatttcg ggtctgggcg gtttgctttt cagacggcag ggcggtcagt tgccgtccag
     6001 ccagcggtcg cgtgtggttt tggcttcttc aaaatagcgg tacagggctt cgcggtcgtc
     6061 ggtggtcagg atgtttgcca aaacgtccaa ctgtttgccc aagccttgaa ccagttgcag
     6121 caggctgtct ttgttggcaa ggcagatgtc cgcccacacg gcgggatgac cggaggcgat
     6181 gcgggtgaag tcccgaaagc ccgtggcggc gaatttcaga tattcctgtc cgtcggggtg
     6241 gtcgagaatc tggtggacat aggcgaaggc ggtcaggtgg ggcatatggg agacggcggc
     6301 gaaaaccgcg tcgtggcgtt gcacgtccat cgtataaatt tccgcaccga ccgcgtgcca
     6361 caggttttct atcaaggcaa tgccgtctga atgttcgccg ccgtgtggcg tgatgatgag
     6421 ttttctgtgg cggaacagcc cgaactgcgc ggcttgcgca ccgcttctgt ccgaaccggc
     6481 aatcgggtgg gcggcgatgc agcggtgcag gcggtcgggc agacagcggc ggaaggcttc
     6541 gatgaccgaa gatttggtgc tgccgacatc ggaaatccaa gtgtgttccg gcaaaacggg
     6601 gcgcagcgcg gtcaaaatgg cgggaaaggt ggcgacgggc gtggcaatca gtaccaagtc
     6661 cgcaccgccg atgctgtccg cgtcgatggc aacggaggca cggtcaatca cgccgcgttc
     6721 caatgcacgt tcgaggttgt cgcggtcggt gtcgataccg gtaacggtgc ggacgagtcc
     6781 ctgccttttg aggtcgagaa cgaacgaacc gccgatcagc cctacaccga tgagggcaat
     6841 atggttcaaa atgggcattt gtgtaaacgg ttttcgcaaa gtaccgtcat ggtagcctat
     6901 cggcggaata tgccgcaagg tcggcaggaa aaaggagaag aaatggacaa aatcagagtt
     6961 gccgccgtgc agatggtgtc gggcgtgtcg ccggaaacca acgtcgctgc catgaaacgc
     7021 ctggtcgcac gggcggcgga gcagggtgtg gattgggtgc tgctgcccga atattgggtg
     7081 ctgatgggcg caaacgatac cgacaaactc gcgcttgccg agcctttggg cggcggacgc
     7141 tttcagacgg cattgagcga aacggcgaaa gaatgcggcg tggtgctgtt tggcggaacc
     7201 gtgccgctgc aaagccccga ggcgggcaag gtcatgaata ctttattggt gtacgggcgc
     7261 gacggcaaac ggacggggct gtaccacaaa atgcacctct tcggtttttc cggtttgggc
     7321 gaacgctatg ccgaagccga taccatccgc gcgggcgggg atgtgccgca cttgtcggca
     7381 gaaggcgtgc cggtggcggc gggcatttgt tacgatgtcc gctttcccga atttttccga
     7441 cgccagttgc cgtttgacgt attgatgctg cccgctgcgt ttacgcacac gacgggcaag
     7501 gcgcattggg agctgctgct gcgcgcgcgt gccgtcgaaa accaatgtta cgtcgtggcg
     7561 gcggcacagg gcggtttgca cgaaaacgga cggcgcacgt tcggacacag catgattgtc
     7621 gatccgtggg gcgacgtgtt ggacgtattg cccgagggcg aaggcgttgt tacggcagac
     7681 atcgatgcca accgcctgaa cagcgtccgc aaccgcctgc ccgccttgaa ataccgggtt
     7741 ttggatgccg tctgaaggtt cagacggcat cggtgccggg gaatcagcgg gctgtaggat
     7801 gggcttcagc ctgccatttc catcaatccc acatttctac cgttttcatc gaatccatcg
     7861 aatccgcccc tttcgacgac ccagtcctac gcaactgaac cgtcattccc acggaagtgg
     7921 gaatctagaa tctcgggggt ttcagtcatt tccgatagat tcccgccgcg tcaggggtct
     7981 ggattcccgc ctgcgcggga atgacgaatc catccatacg gaaacctgca ccacgtcatt
     8041 cccacgaaag tgggaatcta gaacgagggg tttgagcaac tgtttttatc cgataagttt
     8101 ctgtgcggac aggtctagat tcccgcctgt gcgggaatga cgaatttcaa gattacggtg
     8161 ttgtcggaac gaaactgaac cgtcattccc acgaaagtgg gaatctagaa tctcggggtt
     8221 tcagtcattt ccgatagatt ccagccgcgt caggggtctg gattcccgcc tgc
//
LOCUS       DAWKQJ010000081         8133 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000081 DAWKQJ010000000
VERSION     DAWKQJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.081"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            296..520
                     /locus_tag="WJL20_001310"
     CDS             296..520
                     /locus_tag="WJL20_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7683463.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            533..940
                     /locus_tag="WJL20_001311"
     CDS             533..940
                     /locus_tag="WJL20_001311"
                     /inference="COORDINATES: protein motif:HMM:NF025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEZ7683464.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            983..1186
                     /locus_tag="WJL20_001312"
     CDS             983..1186
                     /locus_tag="WJL20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEZ7683465.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1362..2525
                     /locus_tag="WJL20_001313"
     CDS             1362..2525
                     /locus_tag="WJL20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7683466.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            2532..2738
                     /locus_tag="WJL20_001314"
     CDS             2532..2738
                     /locus_tag="WJL20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683467.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2762..3097
                     /locus_tag="WJL20_001315"
     CDS             2762..3097
                     /locus_tag="WJL20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ7683468.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            3133..5229
                     /locus_tag="WJL20_001316"
     CDS             3133..5229
                     /locus_tag="WJL20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ7683469.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIVIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLMTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYAENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            5231..6493
                     /locus_tag="WJL20_001317"
     CDS             5231..6493
                     /locus_tag="WJL20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7683470.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6763..7602)
                     /locus_tag="WJL20_001318"
     CDS             complement(6763..7602)
                     /locus_tag="WJL20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683471.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            7643..>8133
                     /locus_tag="WJL20_001319"
     CDS             7643..>8133
                     /locus_tag="WJL20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7683472.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQAIE
                     VDE"
BASE COUNT         2447 a         1510 c         1655 g         2521 t
ORIGIN      
        1 atttgggtaa aatcaggagt aattaggagc ggaaatagac gaaaacctgt gtttgggttt
       61 cggctgttgg gggaagggct tttttgcaaa ggtctcaatt taaactttaa cgcaatatca
      121 actcattttc tgccacatat ctcaaaaaag tctagtttga tacagtaatc tctttaacag
      181 cttttcttgc taaaatttaa aacaaaatgt aaatattctt ttatttattt aaataataga
      241 ttaaaatatt atttaattaa aagcataatg agcgtagata ttcaaaggag ctaacatgaa
      301 agttatttcc gtattaaatc aaaagggtgg aagtggtaaa accacagtcg caactcactt
      361 ggcacgcgcc ttgcaattag caggaaatag catcttattg gttgatagcg atccacaagg
      421 aagcagtagg gactgggctg ctgttcacga agagcaacct gttcctgtga ttggtattga
      481 cagcccgaca attgatcgtg atttaaaaag cgttgtttaa aaagattttg ttattattga
      541 tggcgcacca caaacagccg atttagccat atcagcgatt aaagcctctg atttagtttt
      601 aatccctgtt caaccgtcgc catacgatat ttgggcaacc gctgatttgg ttaatttaat
      661 caagcagcgg ataaaactca tagatggaaa tttaaaggct gcttttttaa ttaaccgaac
      721 tattaaagga acaaagattg gcggagaaat tagagcagcg ttagaagaat atgagttacc
      781 tgttttatca accgtgattg tgcaacgtat tatttatcca acatctgcta tcacaggtag
      841 cactgtactg gataaagaac caaatggaga agcagctaag gaaattcaat cacttgcgaa
      901 tgagataata aatttgataa atatagataa tcatatttaa tgaaatatta ttttaaattt
      961 ttaaatattg taggaaataa ttatgaacgg attaaaagca gatcgtccta gtgcaaacaa
     1021 agcaaactta tctattgatg atgtgatcaa gcaagaagaa atcaagagaa tcagttttga
     1081 tatgtctgaa tctctgcata gagaattgaa gaaatacgcg gctgagaatg gtgtaactat
     1141 tcgcgtcgtg ctcaataaac tcgtttcaga tttattgaat aaataataac acacggcaga
     1201 atgtatttga ccataatgtc aaagcaatat ccaactttat ctaaaaataa caaaatacct
     1261 tcttccaaaa acagaacgtc ttttcattgc aaaacttgac atataaacac tagttaagta
     1321 aacttaattt tcattattaa taatgaaaat aaggattatt tttgcatatt aacttttcaa
     1381 tggaatataa agaatttaat gaaaattatt tatataaaaa gccatttatt tttaaaaatg
     1441 ctttagacgt aagttctatc tcatggaaag aaattaatga gttgtatcaa cgtgcagacc
     1501 ctactgattg gcaatttaaa tttcgcaaag gggaaataat ccccaaagaa gcctatgttg
     1561 aatcattcaa tgatgtgggt agaatacgtc atcggtttaa taaaacagcc gtatatcagt
     1621 atctgcaaga tggtgcaaca atggtttata accgtattga taatgagcca tttgttgaca
     1681 gtattgctaa acaaattgcc cagtttgctc aagcacaaac tgttgttagt ggttatttgg
     1741 cgtttggtag ttctccatca tatcgtaatc attgggatac tcgtgatgtg tttgcggttc
     1801 aattgattgg caaaaaacac tggacagttt ctgctccaaa ttttgatatg ccattgtata
     1861 tgcagcaagc aaaagatatg ccgcacatta ctccgcctac aacagtagat atggaagtca
     1921 ttttagaagc aggagatatt ttatatatcc cgcgtggttg gtggcataat cctatgccta
     1981 tgaattgtga aacattccat ttagctattg gaacttttcc gccaaatggt tataattata
     2041 tggaatggtt gatgaaaaaa atacctgata tccagagtat tcgtcaaaat ttcatagact
     2101 gggaacacga ccaaaaaaat atagataatg cggctcaggc agttactgaa atgatgaaga
     2161 accaagaaaa ttatcaagca ttcatacaag attttttggg aaatcaacgc gtaaatactg
     2221 catttaatat gcaaattttt ggaaatctgg ataatgacag attacctgaa aatagtacaa
     2281 ttaaactcaa ttcgttggat aaccgtacaa ttaaacaagg atatatcata gcgaatggca
     2341 tcaaaaccaa cttggacaat gatagccaaa cgattttgca atggattgct gacaaacata
     2401 gtgttaagtt aactcaactg tatgaatttt gccaaaatca aaacattaat ttggaaaaag
     2461 tggaaaaatt agtgtttgat ttgacaatga tcgatgtatt ggaatgttta actgatgaaa
     2521 gataaattat tatgtttact tttttaaata aaaatatatt tatcaaaaca attgtacttg
     2581 gctgttttta ttccttttta ttttttagtt tttttgtatc tggtaagtca tttggttatt
     2641 catttgaaat ttttaatatg gatacaagtg cgcttgcttt tttaagtgta tttatgggct
     2701 tttttataag cgtggctgtg catatttacg atatttaata caattgaaaa gataaataat
     2761 tatgaaagaa ttaactatta atgatttaac cctagtttcg ggtggatttg aatttggtcg
     2821 tgattatatt gaaatagctc cagttgtagg tacagcaggt ggcgttgcct cttctgtgag
     2881 agcagcttca atgactggag aagctggagc aattcttact cgttcaggag taacaggatt
     2941 acgtgctgga gtggtagcta ttccattgtt agcctacaat ggtactgtat gggttaatag
     3001 aaataccaaa ttacaagata atttaggtag attaatagat aaagtaactg gcctggatca
     3061 atcaggcaat gattatggcg atgggacaag ttattaatct cataatactt taaaaatcaa
     3121 aaaagaacac caatgtcaat atatctatct cgcctatcat tcggcttttc tcgccgttta
     3181 ctagttgtct tgcaaacgga agcagcagaa tgtggattgg cgtgtttggt atcggtattg
     3241 gggtttcatg gtttttatac ggatttgcgc catttaaggg cgcgtttttc tttgtctctt
     3301 aaaggcgcaa cattggcaga tttggtacgg tttgctaata gcatgaattt aactgctcga
     3361 gctgttcggt tggatttaga tgaattggta aatttgcgct taccctgtat tttacattgg
     3421 gatttaaatc attttgtggt gttgcatgaa gttcatcgta atcacattgt gattatgaac
     3481 cctgcttcgg gttggcaaaa agtgcgtatg gaagaagtct cgcgctgttt tacaggtgta
     3541 gcgttggaac tatttcctaa cacgcaattt gaagagaaac acgaaacgcg taaaattcgc
     3601 gtactaccga tgttgcgcgg agtggtggga ttaaaaaggt ctttatttca gcttctgctg
     3661 ctggcggcag tattacaggt gtttgctttg gcaagccctt tttttatgca atgggtaata
     3721 gaccatgcca ttgtatcggc agaccgtgat ttattgatga cattggcatt gtgttttggc
     3781 ttgttgatga ttgtgcggca gttagtgtca ttgttgcaaa cttgggcggg tatgtactta
     3841 tctacctctt tgaatattca atggaaagct aatgtattac gccgcttaat ggatttgcct
     3901 gtttcttatt tcgctaaacg acatttgggc gatgtggttt cacgttttgg ctcagtggac
     3961 agtattcaaa gtacgctcac ttctactttc tttgtgttgg tgctcaacag cattatggcg
     4021 gtatttacat tagggttgat gtatttgtat agcccaagtt taacgacagt tgtactggtt
     4081 gttttgctga tttatattgg tattcgttgg gtggcatatt atccattacg ccatgcaact
     4141 caagaaaata ttgttcatgc tgccaagcag agcagttatt tcatggaaac catacgtggt
     4201 attcaaactg ttaaattatt tgataaaaat gcacaacgcc atgcggcttg gctgaacttg
     4261 tttattgaca cgattaacac aggtttaatg acacaaaaac tttccgcgct gttcggtttt
     4321 acgaatggcc ttttgtttgg tatcgctaat attttaattg tgtattttgg tgcggtttct
     4381 gtattgaacg gtggttttac cgttggggcg ttaatggcgt ttatgtccta caaaagtcag
     4441 tttgagagta aagcaggttc attgattgac cagtttgtac aaatcaaaat gctaggatta
     4501 cacgcggaac gcttggcgga tattgtgcta gaagaaacgg aaaatgaaca aaacaacgat
     4561 ttcagtctgc ctaaaaattt agaacatttt gatattcaaa tagaaaatgt atcatttagc
     4621 tatgctgaaa atgaaccata cattttgcaa aatttcagtt taatcatcaa acaaggaaca
     4681 gccattgcgt ttgctggaca ttcaggctgt gggaaatcta cgctcattca aattctgaca
     4741 ggcagtctga aacctgaaag cggtcatgtg ttattaggtg ggcatgacat tcatgcgtta
     4801 ccacctgcat ttatccgcac atggagcgca agtgtgatgc aaaatgatgt gttatttact
     4861 ggctcaatcg cagaaaacat cagttttttt gatgatacgc ccaatatgga aaaaatcgca
     4921 ttttgcgctc aaatggcgaa tattcatcat gaaattgtcg ctatgcctat ggcgtatgaa
     4981 acattgattg gcgacatggg gagtgcattg agtggtgggc aaaagcagcg catcgtacta
     5041 gctcgtgctt tatatcgtga accaaaaatt ttgtttttag atgaagccag tagccatttg
     5101 gacatcaaca atgaacgtgt gattaacgat aatctacgcc aactcaacat taccaaaatc
     5161 atggtcgcgc atcgtcagga aactttaaat actgctgata gcgtagttta tttagacaag
     5221 gtagcctgaa atgacattat ttcgccccga agtattccaa gctaaaaaaa atcgctggac
     5281 agggcaaatt gttttagtgc gtcctttttc cttgcagttt ttaacatttt tcgcggtctc
     5341 attagcggct attttagttg cttttttaat ttttgggagc tataccaata aaaccaccgt
     5401 aacaggacaa cttttgccga caacaggggt agtacgcgta tattcgcagg atatgggggt
     5461 tattgctcat caacacgtta tgaatggcga ttttgtgaaa aaaggcgatg tgttatttac
     5521 attatctact tcacgaaatg acaataatgg cagcatacaa gcacgattac tcgccgaagc
     5581 tgaattaaaa aaatcactat cagaacaaga aattattatg aaaaaacgtg ttcatgcagc
     5641 agaaaaaacc gcacaagaaa acacggtaca ccgttttcag aaccaaatgc aacacgttag
     5701 aaatcaaatt atcatgcaag aaaaacgtat tgcgatttct gaaaaaatgt tagaaaaaca
     5761 gcgttattta gccaaaatgg acgcaatttc tgaattagaa aaaaatagtt atgaaattgc
     5821 tttattggag ctgaaagcag gtttagccgc ttaccaacga gaagcagata atcttgctcg
     5881 ggaaataact gtacagcaaa gcaatctaaa aaatctgcct gaacagcaag ctactgaaat
     5941 cagtcaatta gaacgcgcag tatctgtcta tcaacaagaa atattggatt atcaacaacg
     6001 taacgaacaa actattcgcg caaccatatc aggctatgtc agttctatta acacggaaat
     6061 tggtcagcag gtagatacta acaaactatt gatgagtatc gtgccgaaag aaagtgaatt
     6121 actggctaat ttatatgtcc ctagtcgcgc aatcggtttt gtaaaaccaa atgacaaggt
     6181 aattttgcgt tatcaagcat atccttatca aaaattcgga cacgcagaag gacacgtcat
     6241 ttctattgct caaacggcac taggggcgca agaatggaca aatttaggca atatttttac
     6301 tcaaacagca caagtgaatg agcctgttta tttaatcaaa gtaaaactgg ataatcagca
     6361 tattcgtata tatggcacgg agaagaaatt gcaaattggt atgatactag aagccgatat
     6421 tttgcatgag aataaacggt tatatgaatg gatacttgac cctttgtatc aagtaatggg
     6481 aaaaatatca tagctgaatt gctcaataca ttggagcgta tgagtgcccc taattatcaa
     6541 gcaaaaccaa tgtccgatga cttccaattt gttgttaagc acaaatacac agtgttttat
     6601 gtaataatga agacagagaa gtgatacaat ttttcgctcc atttttgctt cctgaattgc
     6661 tgcaattgta tctgcgttgt atgcgagaag taaatatttt cgatgcattt gctgactttg
     6721 taccataata cgtcttccaa ccagtatgat aggctggttt ttctacatgc cgatgccgtg
     6781 gccgtcgtcc cgctccatac gccgcgcctg ccgctcctgc tctttgcgct ggtcttcccg
     6841 ctccttctgc tctacttccc ttatccgctg cgcgccgaaa taaaacgcga ttcgcttggc
     6901 cgaagggctg ccgttcaggg ggaatttttc cagttcgatt tgaaccgcct tctgccctat
     6961 tgtcgtctgt gcttcttggg ctgcctgcaa aaatacgttc ctgaattctt ccatcggccg
     7021 tttgccgatt tccctgcgga cattgtccag ttcttcgtta aacctgtcgc gtactttgac
     7081 cacgtcggcg gccaacttgt cgctatactg cccttccagt ttttcgtttt ctgttttcag
     7141 ccgccggatt tctcgcacgt tcgctccatg ccgttcgttg atgtgttgct gcttctcgtt
     7201 ttccttaacc agttttgcgc cgattttacc catgccgaaa aagcggtttt cgccttggtc
     7261 ttggactgcc caatagtcgt gatgcagccg tttgttgtcg tcttccagcg cccttatttt
     7321 gtttcggttt tcgttcatgc ggtatcgcgc attgatgtac caatctccgc gttctatatc
     7381 gcgctccaag ccgcgcatgt cgtccagttg tttttgaaac agcctgtata tcgtcattag
     7441 cttgtcgttc ggcagcttgg cgataaagtg ctcttcctgt ttcggcggct ctttcggtgt
     7501 ttcctgcgcc tgctttgccc gctgctcggc ggcttgggca gcccgattat aattaatttt
     7561 atcccagaat tcacaatttg agacctttgc aaaattcccc aaaatcccct aaattcctac
     7621 taagacattt aggggatttc tcatgagtac cttcttccag caaaccgcac aagccatgat
     7681 tgccaaacac atcgaccgct tcccactatt gaagttggat caggtgattg attggcaacc
     7741 gatcgaacag tacctgaatc gtcaaagaac ccgttacctt cgagaccacc gcggccgtcc
     7801 cgcctatccc ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctttc
     7861 cgatcccgaa ctcgaacaca gcctcatcac ccgcatcgat ttcaacctgt tttgtcgttt
     7921 tgacgaactg agcatccccg attacagcac cttatgccgc taccgtaact ggctggcgca
     7981 agacgacacc ctgtccgaat tgctggaact gattaaccgc caactgaccg aaaaaggctt
     8041 aaaagtagag aaagcatccg ccgctgtcgt tgacgccacc attattcaga ccgccggcag
     8101 caaacagcgt caggccatag aagttgacga aga
//
LOCUS       DAWKQJ010000082         8116 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000082 DAWKQJ010000000
VERSION     DAWKQJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.082"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1502)
                     /gene="zwf"
                     /locus_tag="WJL20_001320"
     CDS             complement(57..1502)
                     /gene="zwf"
                     /locus_tag="WJL20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ7683473.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2018..3853
                     /gene="edd"
                     /locus_tag="WJL20_001321"
     CDS             2018..3853
                     /gene="edd"
                     /locus_tag="WJL20_001321"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ7683474.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQDTVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDALLESEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NNTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDPIRFDSVTGELNVLINETE
                     WNAREVEQIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            4034..4672
                     /locus_tag="WJL20_001322"
     CDS             4034..4672
                     /locus_tag="WJL20_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ7683475.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            4687..4905
                     /locus_tag="WJL20_001323"
     CDS             4687..4905
                     /locus_tag="WJL20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683476.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(4929..5753)
                     /locus_tag="WJL20_001324"
     CDS             complement(4929..5753)
                     /locus_tag="WJL20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEZ7683477.1"
                     /translation="MWVFEKAKKPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVII
                     SCVSKCCTCDNCKIQLYSHCCNGGWILGYMIDGTQAEYVRTPYADNSLVPLPDNVNEE
                     VALLLSDALPTAQLYSPAAIIVCDMDENRLKLAKELGATHTINPASGDVSKQVAAIVG
                     EDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKLEKLWIKKLAITTGL
                     VNANTTEMLMKAISSSSVDYTKMLTHRFKFSELEKAYDVFKHAAENQAMKVVLEAD"
     gene            complement(5740..5874)
                     /locus_tag="WJL20_001325"
     CDS             complement(5740..5874)
                     /locus_tag="WJL20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine acetyltransferase"
                     /protein_id="HEZ7683478.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     "
     gene            6037..7077
                     /gene="mutY"
                     /locus_tag="WJL20_001326"
     CDS             6037..7077
                     /gene="mutY"
                     /locus_tag="WJL20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ7683479.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            7494..8054
                     /locus_tag="WJL20_001327"
     CDS             7494..8054
                     /locus_tag="WJL20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ7683480.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         2037 a         2242 c         1927 g         1910 t
ORIGIN      
        1 tttacttacg ttcagacggc atttcaaacc ccatgccgtc tgaacgcatt attgtattac
       61 tgctcttcgt gccacttgtg tccgtcgcgc gccaatagtt cgcgcgcggc ttcaggcccc
      121 cacgagtgtg cgccgtagcc gtgcggcggc gtggtgttat ttgtccagtt ttccaaaatc
      181 ggcatcacat attcccacgc ggcttcgagt tcgtcgcggc ggttgaataa agcgagtttg
      241 ccgttaatca catccagcag caggcgctcg taagcttccg cgcggcggct ttccaatgct
      301 ttgcccaaat cggttgccag cggcacggtt tcgaccttat ttcctgcccc cggggttttc
      361 atctgcgtat agaggcgcac ggattcatat ggttgcaact cgataacgag ccggttgggc
      421 gcggtgcggc tgccttcaaa aatatggctg ttcaaatctt tgaagttcaa aacgatttcc
      481 gccactttgc ccgccatgcg cttgccggta cgcaggtaga agggaacgcc cttccagcgt
      541 tcgttttcga tttcggcttt aatggcgacg taggtttcgg taaagctgtc ttgcggaacg
      601 ttgatttctt caagatagcc gttcatgcct ttggcggcgg tatattgtcc gcgcacgaca
      661 ttttcattga cagattcgac ggtcagcggc ttcaatgact tgatgacttt gactttttca
      721 tcgcgcaccg cgtcggcatc caagctggcg ggggcttcca tcgcagtcat gcacagcatt
      781 tgcatcaagt gattttgcac catatcgcgc aacgcgccgg taatgtcgta aaactcgccg
      841 cgttcttcca cgcccaactg ctcggcgatg gtcaactgca cgctttcgat gtatttattg
      901 ttccacagcg gctcgaacat tacattggca aaacgcagcg caagcaggtt ttgcaggctt
      961 tctttgccaa ggtagtggtc gatgcggtaa atttgccctt ctttgaaata acgcgcgaca
     1021 tcggtattga tttgctggga agaagccaaa tccgtaccca acggtttttc caaaaccaca
     1081 cgcacgttgt cggcgttcaa accgattttg gcaaggtttt cacatgtttg cgcgaagaat
     1141 ttcggcgcgg tggaaagata gatgacgaca ttgtcggttt ctttgcgcgc tttgaccaaa
     1201 tcgcccaaag cggcaaaatc gtccggctgc gtaacatcga ctttgagata tgcgaaacgt
     1261 tcgacaaacg atgcccaagc ctcatcggaa aaattttctt tcacatggat tttggaactg
     1321 gtttccacct tcgccagaaa accttcggta tccaactcgc tgcggctgac ccccaaaata
     1381 cgcccttcgg gatgaagcag accggcaaca tgcgcctggt acagacaggg caacagcttg
     1441 cgcatcgcca aatcgccggt cgcaccgaac aacaccaaat caaaatttgt ttgtgtactc
     1501 atcgtattat ctcgtcagga aagaattttt cgatgccgtt tgaaacctgt tttccccatc
     1561 acgctgcatc gcaatatcgg aaacaaaggc aggcggcata atgagtagta atactacaca
     1621 ccgctacact ttttgtctat tcccattttt acaatttatt tgacctagtc caaaaatcgg
     1681 gcaggtttcc cctattccgt tacaacaatc gaaagattct gcgatttaaa tcaaatttct
     1741 tttcgatgtc tgattttttt gtaacaaaat tacaaatttt gtactataat aacacccgct
     1801 tcccactttc agacggtata ccttttaaaa tatagtggat taacaaaaat caggacaagg
     1861 cgacgaagcc gcagacagta cagataatac ggcaaggcga ggcaacgccg tactggtttt
     1921 tgttaatcca ctatactcac cgtctgaata cccgatacaa aaatcagaaa cgcacaaaca
     1981 aatccccaat accccccgtt ccgacaggag accgaccgtg aacactactc ctatccactc
     2041 caaactcgcc gaaatcaccg ggcgcattat tgaacgcagc cgtccgacgc gtgaaaaata
     2101 tctggcgaaa atccgtagtg ccaaacagat gggacgctta gagcgcaacc aactcggctg
     2161 cagcaatttg gcgcacggct acgcctccat gcccaaaagc atcaaaatcg aaatgcttca
     2221 ggacaccgtt cccaacttag gcatcatcac cgcctacaac gacatggttt ccgcacacca
     2281 gccgtttaaa gacttccctg accaaatcaa agacgaagcg cagaaaaacg gcgcaaccgc
     2341 gcaagttgcc ggcggtacgc ccgccatgtg tgacggtatt acccaaggct atgccggcat
     2401 ggaattgtcg ctgttctccc gcgacgtaat tgccatgagt actgctgtcg gtctgtctca
     2461 tcaaatgttt gacggcagcc tgtttatggg cgtgtgcgac aaaatcgttc ccggtttgat
     2521 gatcggtgcg ttgtctttcg gccatattcc gggcatcttc gttcctgcag gcccgatgtc
     2581 cagcggtatc ggcaataaag aaaaagcccg tacccgccaa cttttcgctg aaggtaaagt
     2641 cggccgcgat gccttgctcg aaagcgaaat gggctcttac cacagcccgg gtacttgtac
     2701 cttctacggc accgccaact ccaaccaaat gatgatggaa atgatgggag tgcacctgcc
     2761 tgccgccgca ttcgtccatc cttacaccga cctgcgcgaa gccctgaccc gctacgctgc
     2821 cggacacctc gcgcgcggca ttaaaaacaa caccatcaaa cctttgggcg aaatgttgac
     2881 cgaaaaatcc tttatcaacg ccctgatcgg tctgatggcg accggcggct cgaccaacca
     2941 caccatgcac ctcgtcgcta tggcgcgtgc ggccggcgtg attctcaact gggacgactt
     3001 cgatgaaatt tcctccatca tcccgctgct catccgcgtt tatccgaacg gcaaggccga
     3061 cgtaaaccac tttaccgcag cgggcggact gcctttcgtc atccgcgaat tgctggacgc
     3121 aggcctgttg cacgacgatg tcgataccgt cgtcggacac ggtatgcgcc actacaccaa
     3181 agagcctttc ctcatcgacg gcaaactcga atggcgagaa gcccccgaaa ccagcggcaa
     3241 cgacgacatc ctacgcaaag ccgacaaccc gttctcccca gacggcggtc tgcgcctgat
     3301 gaaaggcaac atcggtcgcg gcgtgattaa agtgtccgcc gtgcgcgaag gctgccgcat
     3361 tatcgaagcg cctgccatcg tgttcaacga ccaacgcgaa gtgttggctg cgtttgagcg
     3421 cggcgaattg gaacgcgatt tcgtctgcgt cgtccgctac caaggcccgc gtgccaacgg
     3481 tatgcccgag ttgcacaaac tgaccccgcc tttgggcatc ctgcaagacc gcggcttcaa
     3541 agtggcgctg ctgaccgacg gccgtatgtc cggcgcgtcc ggcaaagttc cggcctccat
     3601 ccacatgacc cccgaagccc tgatgggcgg caacatcgcc aaaattcgta ccggcgaccc
     3661 gatccgcttc gactccgtta ccggcgaact caacgttctg atcaacgaaa ccgaatggaa
     3721 tgcccgcgaa gtcgaacaaa tcgacttggg cgcgaaccaa caaggctgcg gtcgcgaact
     3781 cttcgccggc ttccgcagca tgaccagcag cgcggaaacc ggtgcgatga gcttcggcgg
     3841 cgaatttgcc tgatgcgcgt ttcagacggc cttttcagac cgaaggccgt ctgaaaaatt
     3901 attcaagcgt tttaagatag acgtaggttg gattctcgaa tccgacacag ccgtccaaga
     3961 tgtcggtttc ttgaatccga cctacaacct gtcccatctt aataaaatac cctatcctac
     4021 ccggagaacc gaaatgtcca aactgacccc ccgcgaaatt ttgaccgccg gcgcagttgt
     4081 gccggtaatg gcgattgacg acttaagcac cgccatcgat ttgtcccacg cccttgtcga
     4141 aggcggcatc cctaccctcg aaatcaccct gcgcacccct gtcggcctcg atgccatccg
     4201 cctgattgcc aaagaggtgc ccaacgccat cgtcggcgca ggtacggtaa ccaatcccga
     4261 acagctcaaa gccgtcgaag acgcaggcgc ggttttcgcc atcagcccgg gtctgcacga
     4321 atccctcgcc aaagccggcc gcaacagcgg cattcccctg attcccggtg ttgccacccc
     4381 gggcgaaatc caactggctt tggaacacgg catcgacacc ctcaaactct tccctgccga
     4441 agtcgttggc ggcaaagcca tgctcaaagc cctgtacggc ccttacgccg atgttcgctt
     4501 ctgcccgaca ggcggcatca gcctcgccac cgcgcccgat tacttggcac tgcccaacgt
     4561 cctgtgcgtc ggcggctcat ggctgacgcc gaaagaagcc gtgaaaaaca aagactggga
     4621 caccatcacc cgtctcgcca aagaagcggc ggcattgaaa cccaaagcct gattcgcatc
     4681 gtaaaaatgc cgtctgaagc cctttccagt ctcagacggc attttgccgc ttggcggcgc
     4741 agtcggcata cacggcagca ccactccgac atactgcccc taaaatgccc atccgccttc
     4801 cgcataataa aaataacgtt cagttcattc gacagcagcc ggacagccca tactacgcgg
     4861 ctgaaaaaat gccgtctgaa acgcattcag acggcatccg cttaaaaaaa caactgattc
     4921 aaggccgatt aatccgcttc caaaaccact ttcatcgctt gattttcggc ggcgtgtttg
     4981 aacacgtcgt aggctttttc caattcgctg aatttgaaac ggtgggtcaa cattttggtg
     5041 taatcgacgg agctgctgga aattgccttc atcagcattt cggtggtatt ggcgtttacc
     5101 aggccggtag tgatggcaag ctttttaatc cacagttttt ccagtttgaa atcaacggat
     5161 tgaccgtgta cgccgacaac ggcgatatgg ccgccgggtt tcacaatgtc ttggcacata
     5221 ttccaagttg cagggatacc gacggcttcg atcgcgcagt ccacgccgtc ttcgccgacg
     5281 atagcggcaa cttgcttgct tacatcgccg gaagcagggt taatggtatg ggttgcgccc
     5341 aactctttcg ccagtttcaa acggttttcg tccatatcgc aaacaatgat ggcggcgggg
     5401 ctgtacagtt gggcggtcgg caaggcgtcg ctcaacaaca gggcgacttc ttcgttgacg
     5461 ttgtcgggca gcggcacgag gctgttgtcg gcataaggcg tgcggacgta ttctgcctgc
     5521 gtgccgtcaa tcatgtagcc caaaatccag ccgccgttgc agcaatgcga ataaagttgg
     5581 attttgcagt tgtcgcaagt acagcatttg ctgacgcatg aaataatgac tttatcgccg
     5641 actttgatgt tttttacagc ctcgccgact tcttctacaa taccgatgcc ttcatgaccg
     5701 agaatacgac cgtcggcgac ttcgggtttt tttgcctttt caaataccca aatcggtacc
     5761 gcaaatcgtg gttttgacga ttttcaccac cgcatcggtc ggatcgataa tctgcggacg
     5821 gggtttttct tcaaaacgga tgtcgtttgc gccgtgataa accattgctt tcatgctgat
     5881 actccttgct tgttgataaa taatttcaat accgcaataa agtttcttta tatgagttat
     5941 atacccctac aaaaaataag tcaataagaa ttattttcac aatgttatac aataacatac
     6001 cgttttaaat ataaataaaa ccaccgattg atattaatga acacacccat ccccttctcc
     6061 gaacggctca tccgctggca aaaacaacac ggtcgccacc acctcccttg gcaggtcaaa
     6121 aacccttatt gcgtctggct ttccgaaatc atgctccagc aaacgcaagt cgccaccgtg
     6181 ttggactact atccgcgctt cttagaaaaa ttcccgaccg ttcagacgct tgccgccgcg
     6241 ccgcaagacg aagtgttgtc gttgtgggcg ggcttgggct attacagccg cgcgcgcaac
     6301 ctgcacaaag ccgcgcaaca agtcgtcagg caattcggcg gcacgtttcc gtcggagcgc
     6361 aaagacttgg aaaccctctg cggcgtaggc agaagcaccg ccgccgccat ttctgccttc
     6421 tccttcaacc gccgcgaaac cattttggac ggcaacgtca aacgcgtact ctgccgcgtg
     6481 ttcgcccgcg acggcaatcc gcaggacaaa aaatttgaaa actcgctctg gacacttgcc
     6541 gaaagcctgc tgccgtctga aaacgccgat atgcctgcct atacacaagg tttgatggat
     6601 ttgggcgcga ccgtgtgcaa acggacgaaa cccttgtgcc accaatgccc gatggcggac
     6661 atctgcgaag caaaaaagca aaaccgcacc gccgagctgc cgcgcaaaaa aaccgccgcc
     6721 gaagtaccga ccctgccgct ttactggctg attgtccgca accgggacgg cgcgattttg
     6781 ctggaaaaac gccccgccaa aggcatttgg ggcgggctgt attgcgtgcc gtgttttgaa
     6841 agtttgaacg ggctttccga ctttgccgcc aaattctccc tgaccatggc agatatggac
     6901 gaacaaaccg ccctgaccca ccgtctgacg caccggctgc tgatgattac gccgtttgaa
     6961 ggacaaatgc cgtctgaaag cccttcagac ggcatttgga taaagccggc gcatttgaaa
     7021 gattacggcc tgcccaagcc tttggaaatt tatttaaacg gtaataggtt agaataaaca
     7081 aaataaaccc attgaactgt tgtttgcagg tatcgcagca agaacaaccg atgaatttgg
     7141 gtcgtatttt aggcggtggg ataatgttca aatgggacat ttggaacgga agaagtcggc
     7201 aatttaaaaa ggatttaaaa agcaaagaag gtcaaaaaca tgaacacaaa cttaaatgac
     7261 aaagacaaag ccatggatac cgcaatcagg tttcagaaaa ggatgaggat tccgaaattt
     7321 ttctttttaa ttctctgaat cacaatggtt ttggcattta tccaagacgt gataacgggt
     7381 tctaattttc tgcaaataac aattagtgta aaattttcgt aaaaatttat cggcttttaa
     7441 aacaaaattg actaaaatag tcgcgagttt ttactgcaat aaaggagatt gcaatgaata
     7501 tgaaaacctt attagcacta gcggttagtg cagtatgttc agttggtgtt gcgcaagcac
     7561 acgagcataa tacgatacct aaaggtgctt ctattgaagt gaaagtgcaa caacttgatc
     7621 cagcaaacgg taacaaagat gtgggtacag tgactattac tgaatctaac tatggtcttg
     7681 tgtttacccc tgatttacaa ggattaagcg aaggcttaca tggtttccac atccatgaaa
     7741 acccaagctg tgagccaaaa gaaaaagaag gtaaattgac agctggttta ggcgcaggcg
     7801 gtcactggga tcctaaaggt gcaaaacaac atggttaccc atggcaagat gatgcacact
     7861 taggtgattt acctgcatta actgtattgc atgatggcac agcaacaaat cctgttttag
     7921 caccacgtct taaacattta gatgatgttc gcggtcactc tattatgatc cacacgggtg
     7981 gtgataatca ctccgatcat ccagctccac ttggcggtgg cggcccacgt atggcatgtg
     8041 gcgtgattaa ataattcgat tgttcgaaac gaaaagtgcg gtgaattttg accgcacttt
     8101 tttgctagat atttag
//
LOCUS       DAWKQJ010000083         7987 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000083 DAWKQJ010000000
VERSION     DAWKQJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.083"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="WJL20_001328"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="WJL20_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ7683481.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(572..1363)
                     /locus_tag="WJL20_001329"
     CDS             complement(572..1363)
                     /locus_tag="WJL20_001329"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ7683482.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1412..1819)
                     /locus_tag="WJL20_001330"
     CDS             complement(1412..1819)
                     /locus_tag="WJL20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ7683483.1"
                     /translation="MGMRTIKYLAMALLLMFTVAGCQLAGWYPCPSLSGWCMPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSECVRW"
     gene            complement(1801..2505)
                     /locus_tag="WJL20_001331"
     CDS             complement(1801..2505)
                     /locus_tag="WJL20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ7683484.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQVGRDPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTIC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRSRLECVQ"
     gene            2625..3668
                     /locus_tag="WJL20_001332"
     CDS             2625..3668
                     /locus_tag="WJL20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ7683485.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3831..5057
                     /locus_tag="WJL20_001333"
     CDS             3831..5057
                     /locus_tag="WJL20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7683486.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5343..7493
                     /gene="yccS"
                     /locus_tag="WJL20_001334"
     CDS             5343..7493
                     /gene="yccS"
                     /locus_tag="WJL20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ7683487.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAILLFQIVLPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDNPDIRHLGRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESSVFRHAVRLS
                     LVVAAACTIVETLNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALNTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1983 a         2621 c         1733 g         1650 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatgctcg
      361 tcattcatat aatcgtcttc cggcccgttc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atatcctctc ggttcaccga aaaaaacaaa gcaccacaaa gtggctttat
      481 tcctttgatt tttataaggc ttattgccta gggcgttttt gaaaacgcgc atcataacac
      541 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      601 aacggcgcac acaggcacaa acgccctcgc ttatggcttc ggcgacacga tgccgcctga
      661 aagcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      721 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      781 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc agcattttgc aatgcgtcca
      841 gcagataaaa cacataagcc ggtccgctgc cgctgatacc ggtaatgctg tgcatttgcg
      901 cttcatcttc caaccaaacg gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
      961 ggtctgtttc cgatacttcc gcttcggcaa acataccgga tacgcccagc ccgatttttc
     1021 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1081 tatcaaccga caatccagct gcaacagaaa gcaccaatgc gccgttggtg cggatatttt
     1141 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaatgaa
     1201 gctccggcaa ggtttcagaa gtttcgaccc ccaactcttt ttgcaaacgt tcgcgctttt
     1261 ccgcaccccg gttggcaata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1321 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1381 aaattacctt actaaattca ttcccaattt tttaccatcg gacacactcg gacagaccgt
     1441 tgcgccgcaa cccctgcttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1501 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cctattttcc
     1561 tgtaaaagta agattgttgt gcggattcat attcgtttgc cctaacggaa cgattgccgt
     1621 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1681 aatcgatggc agccggtttt ctcggcatac accagccgga caacgacgga catggatacc
     1741 aacccgccaa ctgacagccg gcaaccgtaa acataagcaa taacgccatt gcaagatatt
     1801 ttattgtacg cattccaatc tgctcctttt cccgaaaatc gcactgccga tacggacatg
     1861 tgttgcaccg cacgcaatgg cggcaggcat atcgtccgac atccccatag acagcacatc
     1921 cgccttaacg ccagccgtat tgaggtcggc aagcagtttc cgcatcgttt gaaattgcac
     1981 cttcaactcc gtttcactgc tgttggcttt ggcaacacac atcagtccgc gtacgacgat
     2041 attcggcaac ttcgccactt cgaccgcaag cacgaccgct tcttcgggcg cgacaccgtg
     2101 tttcgccgcc tcgcccgcaa tgttcacctc gatacacacc tgcaaaggcg gcattgagga
     2161 aggacgttgc ccgctcagcc ggacagcggt tttcagacgg catatggtat gcacccaatg
     2221 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2281 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2341 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2401 accgacggca atcaggctga cggaatgcgg atccctgccc acctgcagaa ccaattttcc
     2461 gatacggtcg gacacctcac aataacgttc ttgcaacacc gtcatagatt atcccctaat
     2521 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2581 accattccaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2641 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2701 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2761 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2821 acttggaagt cgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2881 tcaacatcgg ccgcggtccc gccgccgtat tccgcaccat tcccagcacc gtcttatcgc
     2941 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3001 ttttggttac cggccctacc ggttcgggca aatcgaccac gcttgccgca atgatcaact
     3061 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatc gaattcgtcc
     3121 atcaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3181 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3241 gcgacccaga aaccattggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3301 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tattgtggac gtattcccgg
     3361 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3421 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3481 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3541 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3601 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3661 gtttctgaca cacaaccgcc ttccggccat accggcggga aaacaaggcg caaacacgcg
     3721 aggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3781 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3841 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3901 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     3961 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4021 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacgggc gaggaaaccg
     4081 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4141 aaatcaacta ttccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4201 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4261 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4321 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4381 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgagttt atctacaaac
     4441 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4501 cggtacaaaa cgctatgcgc cagtcccccg acgtggtctg catcggcgaa gtccgcagca
     4561 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4621 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4681 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4741 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4801 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4861 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4921 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     4981 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5041 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5101 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5161 agtggattaa caaaaatcag gacaagacga cgaagccgca gacagcacaa atagtacgga
     5221 accgattcac ttggtgcttc agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5281 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5341 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5401 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5461 tactcggcat catcgctggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5521 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagcaccc
     5581 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc ttcaccattt
     5641 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5701 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5761 gcggcaccgt actgtacagc accgccatcc tcctgttcca aatcgtcctg ccccaccgcc
     5821 ccgtccaaga aagcgtcgcc aacgcctacg acgcactcgg cggctacctc gaagccaaag
     5881 ccgacttttt cgatcccgac gaagccgaat ggataggcaa ccgccacatc gacctcgcca
     5941 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6001 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgttactac tttgccgccc
     6061 aagacataca cgaacgcatc agctccgccc acgtcgatta tcaggaaatg tccgaaaaat
     6121 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6181 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6241 gccgcgccat cgaaggctgc cgccagtcgc tgcgcctcct ttcagacggc aacgacaatc
     6301 ccgacatccg ccacctcggc cgcctgctcg acaacctcgg cagcgtcgac caacaattcc
     6361 gccaactcca gcacaacggc ctgcaggcgg aaaacgaccg catgggcgac acccgcatcg
     6421 ccgccctcga aaccggcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6481 tcgaatcaag cgtattccgc catgccgtcc gcctgtccct tgtcgttgcc gccgcctgca
     6541 ccatcgtcga aaccctcaac ctcaacctcg gctactggat actgctgacc gcccttttcg
     6601 tctgccagcc taactacacc gccaccaaaa gccgcgtccg ccagcgcatt gccggtaccg
     6661 tactcggcgt aatcgtcggc tcgctcgtcc cctacttcac cccgtctgtc gaaaccaaac
     6721 tctggattgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6781 tctccacctt cttcattacc attcaagccc tgaccagcct ctccctcgca ggtttggacg
     6841 tatacgccgc catgcccgta cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6901 cggcagtcag ctacctatgg ccagactgga aatacctcac gctcgaacgc accgccgccc
     6961 ttgccgtatg cagcaacggt gcctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7021 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7081 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7141 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7201 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7261 tcgccgccga acacaccgcc cacatcttcc aacacctgcc cgaaaccgaa cccgacgact
     7321 ttcagacagc actgaataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7381 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgcccggcag ctcgaaccct
     7441 actaccgcgc ctaccgacaa attccgcaca ggcagcccca aaacgcagcc tgaaaaagtt
     7501 tcggcatttt gtaagagaga gcagattgtc agacaggttg caagatagtg gataaagctt
     7561 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7621 cccacgaaag tgggaatcca gttttttgag tttcagtcat tcccgataaa ttgccttagc
     7681 gttgaatgtc tagattcccg cctacgcggg aatgacgaat ccatccatac ggaaacctac
     7741 atcccgtcat tcccacgaaa gtgggaatcc agaatctcgg actttcagat aatctttgaa
     7801 tattactgtt gttctaaggt ctagattccc gcctgcgcgg gaatgacgaa tccatccgca
     7861 cggaaaccta cattccgtca ttcccacgaa agtgggaatc cagaatctcg gactttcaga
     7921 taacctttga atattactgt tgttctaagg tctagattcc cgcctgcgcg ggaatgacgg
     7981 cagagcg
//
LOCUS       DAWKQJ010000084         7983 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000084 DAWKQJ010000000
VERSION     DAWKQJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.084"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WJL20_001335"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WJL20_001335"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ7683488.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPTSPEQQRLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDALRRFQHAQVFRLAVQDLAGLWTVEPLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3354)
                     /locus_tag="WJL20_001336"
     CDS             complement(3151..3354)
                     /locus_tag="WJL20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683489.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFFKNSTREKGAAYAVLKERGEV"
     gene            complement(3491..3964)
                     /locus_tag="WJL20_001337"
     CDS             complement(3491..3964)
                     /locus_tag="WJL20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ7683490.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4017..4298)
                     /locus_tag="WJL20_001338"
     CDS             complement(4017..4298)
                     /locus_tag="WJL20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683491.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4385..4708)
                     /locus_tag="WJL20_001339"
     CDS             complement(4385..4708)
                     /locus_tag="WJL20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683492.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5041..6048)
                     /locus_tag="WJL20_001340"
     CDS             complement(5041..6048)
                     /locus_tag="WJL20_001340"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ7683493.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6280..6516
                     /gene="acpP"
                     /locus_tag="WJL20_001341"
     CDS             6280..6516
                     /gene="acpP"
                     /locus_tag="WJL20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ7683494.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6672..7919
                     /gene="fabF"
                     /locus_tag="WJL20_001342"
     CDS             6672..7919
                     /gene="fabF"
                     /locus_tag="WJL20_001342"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ7683495.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1850 a         2122 c         2061 g         1950 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatattcaag agggcgatgt
      481 tgccgtagtt ggcgagaagt tgcggatatt ttcgggcatg ggcaaggatg aggtattgga
      541 cgataaattc gacgtcgacc acgccgccgc gtgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgatgatttc gcctgacaag gcggtttggt
      661 cgcgttcggc ggtgaggatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggcg
      721 tgccgcagat gaagcgggcg cgggtaaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 cgacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgagtatg gtgtcggcga gggcggagag ttggtcggag aggggttcta
     1141 ccgtccacag tccggcaagg tcttggacgg cgagacggaa gacttgggcg tgctggaagc
     1201 ggcgcagggc gtccatttgc gcttcagtat cgccgccgca ggcttcgatg ccgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gcgctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggtttgtcct
     1681 gccatgccca ttgccattcg ctgttgtctt gcgtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttgttccgat gaacattgag accgtctgaa aaagcggaat
     1801 aactgtcgaa acccatgctt tcggcgagca gtcgttgctg ttcgggcgag gtcggcaggg
     1861 tttgagtttg ccggtcgtcc caatattgca aacgatgttc aacatcgcgc aggaagcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagcttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcgcgcat ttgtccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagt ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc tgatttggcg gtgcagctta cgcatcgcct
     2161 catacgcgct gtaatccaaa tatttgcgga acacgaaagg gcgcaccagc gatttgattc
     2221 cgttcggata cggcgtaacc acgcggcctt tgcaccacgc gtagcgttcc cattcgcgcc
     2281 cctgcgtaat caaatattgc tccagcgcgg tttcgctcaa taccaacgcg cccgaatcac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgtcc gtcggcggtg atgtcgttca
     2401 gcagcgcaat cagtttctgc ccgactttgg tgaaaaactc ttgattgccc cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggataga cgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tataacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt tatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccagcatagg caggaaaatt tcgggcttga
     2881 gtttgccgtt gtcgagctgg cgggcgagga agagggaatg gcggcgggcg gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccagttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttgc cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaacagc
     3181 ataagccgca cctttctccc gtgtggaatt tttaaaaaaa ccatcactct ttacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattggc
     3301 tgcttcaatc attccctcgc ctactgcttt tgccacacca ttccaaaaac ccataatcat
     3361 gtcctccaat cgtttcaaat aaataaggtc gttgtttctg tcaagaaaca acgacagttt
     3421 ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag acggcatcgg
     3481 attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag tgagaatata
     3541 accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct ttgggcggca
     3601 tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa attttttcaa
     3661 cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt aagaggacgt
     3721 acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc agaacaggct
     3781 ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt tcatcatgat
     3841 ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag tatttattcc
     3901 cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt aaatcaaggt
     3961 tcatttttta ctccgattgc ctgccccgtc attccgcccc ttccgattga cgcctgttac
     4021 gaaaagtctt ccaaatgacg gctgattgcc tcttcaacag tcggatgcca atccataaat
     4081 gaagcctgca attcctctaa aaccaactca ttgaataaat catccaaatt ccgccaatcc
     4141 gttttttcat ccggatagat gaaatttgcc ataacgacag tatgtttgtt ttgggaataa
     4201 tccgataagg cgcaaatcat cagataccgc cccgaatgct ccagataaaa aattcgtttt
     4261 tccaaaggaa accggttatt ccgttgcccg ttttccattt ttcatccttt ttaacgtatc
     4321 ggcttccgcg tccttcaaat tgagttacct gccttccgcc aagcaaacgc ccgcgcccct
     4381 ttcatcattt gaatatccgt ttgaccatat ttaccagata actatcttct ttatataaat
     4441 caatcagcaa aggcacgata cccggctcat aaaataattc tgtcgtatca gatgtttctt
     4501 ttaacgcgga aattatctcc aacagttcat acccgtatcg gggataagca aacaaatagg
     4561 ggactgcttc ccgcaaacat gaaaatgctt cgtaactttg catttcccat atatgttcat
     4621 ctttaaaatc actcaaatac cattcatcca attcactttc cggatcggat atcaatcgga
     4681 taaattcatt tagttcgtcc ttaaacatat caaatcctta aatataaaat cttttgatta
     4741 tccgccgtat ccttattttc cacatcatga atttcaacgc cgttcatatt ttgcgttttc
     4801 ccaagccgtc cgccacagta taaaattatt ttttgtatca attgcttata tatttaacaa
     4861 cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg cgggcgtttg
     4921 ctttgtttca aacagggcgg cgcgtaaggg attttcaagg ggcggcaggc ggaaaaagga
     4981 aatgaaaatg ccgtctgaac ggcaaaacgt gcgtttagac ggcattttgg gcgggacgcg
     5041 tcatcgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt atatcaatcc
     5101 gctgtacact tggacggcgg tcgcgcccaa gcggatttta tctgccgcat ccttgccttc
     5161 cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg ccaacagctt
     5221 caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct gctcgcctgc
     5281 gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga tgatgccgtc
     5341 catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat ccaaatcggg
     5401 ggcgattttg acggcgagcg gaacgtattt cccgtataca gaggcaagct gtgcctgttt
     5461 gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg cgcggaggtt
     5521 tttggtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt aggctttttc
     5581 aaggcagatt aaataatcat cggcagcgtt ttcgatgggt gtaaccgcgt ttttaccgat
     5641 gttgatgccc aatacgccac tgaatttact tttttcgatg ttgcgtatca tggtgtcgat
     5701 accgtggttg ttgaaaccca tgcggttgat gatgccttgg tgttcgggaa cgcggaagag
     5761 gcgcggctgc gggttgccgg gctgcggttt gggcgttacc gtgccgattt cgatgaaacc
     5821 aaagccgagc gcgcccaatg cgtcgatgta ttcgccgttt ttgtcgagtc cggcggcaag
     5881 tccgacaggg ttgggcaaat ccatacccat caattttaca ggtttggtac ggttgccggt
     5941 tacgggaatc agacccaatt tataaaccgt gtagagcgcg tcgagcgtga agtggtgggc
     6001 tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga cagggctttc
     6061 tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac ggcacacagg
     6121 tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca aatattgata
     6181 taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg atttatttcc
     6241 cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat cgaacaacaa
     6301 gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa aaacgaatct
     6361 tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt tatggctttg
     6421 gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac caccgtccaa
     6481 ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg acacaacagc
     6541 ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt gaacccctgc
     6601 cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg acaaccaaca
     6661 gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag gtttcccctg
     6721 tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc cggcaaaagc ggcatcggcg
     6781 cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa gtgcgcggtt
     6841 tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta ttcatccact
     6901 acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat gtggaaaacc
     6961 tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg cccagcatcg
     7021 aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct ttctttatcc
     7081 ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc taccgcggcc
     7141 cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc aattccgccc
     7201 gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa ggcgcaatca
     7261 gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc aacgacgacc
     7321 ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc ggcgaaggcg
     7381 cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg aaaatctacg
     7441 ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg ccgaacgaag
     7501 aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat cccgaagacg
     7561 tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac gaaaccaaag
     7621 ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg accaaatcca
     7681 tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc attttggcga
     7741 tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt gaagccggct
     7801 gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt gccatttcca
     7861 actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc aaaggctgat
     7921 tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac ggcattttta
     7981 tag
//
LOCUS       DAWKQJ010000085         7944 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000085 DAWKQJ010000000
VERSION     DAWKQJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.085"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WJL20_001343"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WJL20_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ7683496.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJL20_001344"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJL20_001344"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ7683497.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WJL20_001345"
     CDS             1963..2286
                     /locus_tag="WJL20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ7683498.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2334..2810
                     /locus_tag="WJL20_001346"
     CDS             2334..2810
                     /locus_tag="WJL20_001346"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ7683499.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRILQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2888..3313
                     /gene="nusB"
                     /locus_tag="WJL20_001347"
     CDS             2888..3313
                     /gene="nusB"
                     /locus_tag="WJL20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ7683500.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAIPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WJL20_001348"
     CDS             complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WJL20_001348"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ7683501.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4930..5076)
                     /locus_tag="WJL20_001349"
     CDS             complement(4930..5076)
                     /locus_tag="WJL20_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683502.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5077..5301)
                     /locus_tag="WJL20_001350"
     CDS             complement(5077..5301)
                     /locus_tag="WJL20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ7683503.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5410..6021
                     /locus_tag="WJL20_001351"
     CDS             5410..6021
                     /locus_tag="WJL20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ7683504.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6081..6953)
                     /locus_tag="WJL20_001352"
     CDS             complement(6081..6953)
                     /locus_tag="WJL20_001352"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ7683505.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEIAGNVKPTDLLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6990..7775)
                     /locus_tag="WJL20_001353"
     CDS             complement(6990..7775)
                     /locus_tag="WJL20_001353"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ7683506.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKGNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNTGNEAAAVGALVKELKDAVR"
BASE COUNT         1913 a         2390 c         1890 g         1751 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc ggcacaccgg acgcggtcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 agaccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatcg ccgacaaact caaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatccgg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgcac tgctttccca acccgaaggc
     2221 agcgcatggg agcttgccga agacggcaca ttccaaaccg tcgccgactg gcagccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac ctccgaatac caaaggacac ccaatgaaca
     2341 ccatcgcccc caacctcgac ggcaaacacc tccgcatcgg catcgtacag gcacgcttca
     2401 ccaacgaaat cggcagcgaa atgctcaaag tctgctgccg catcctccaa gaattgggcg
     2461 tggcagacga aaacattacc gtcgccaccg tacccggcgc gcttgaaatc cccatcgcgc
     2521 tgatgaatct tgcctcttcc gaaaaatttg acgcactgat tgccatcggt gtcgtcatcc
     2581 gtggtgaaac ctaccatttc gagctggttt ccaacgaatc cggagcaggg gttagccgcg
     2641 tcgcactcga ctacaacatc ccgattgcca atgccgtctt aaccaccgaa aacgacgcgc
     2701 aggcaatcga acggattgaa gaaaaagcct cggatgccgc caaagtcgcc gtagaatgcg
     2761 ccaacctcgt caacctgctg ctcgaagaac agtttgaaga cgaagaataa caaacagggc
     2821 gtacaccaat acgccctttc cgtttcagac ggcataccgt gccgcccaac cgtttcaagg
     2881 aaaaatcatg aaaacagccc gccgccgttc ccgcgagctt gccgtacaag ccgtttacca
     2941 atcccttatc aaccgcaccg cagcacccga gattgctaaa aacatccgcg aaatgtccga
     3001 ctttgccaag gcagacgaag aattgttcaa caaacttttc ttcggcacgc aaaccaatgc
     3061 ggcagaatac atccggcaaa tccgcccgct gctcgacagg gacgaaaaag acctcaaccc
     3121 catcgaacgc gccgtcctgc tgaccgcctg ccacgagctg tccgccatac ccgaaacgcc
     3181 ctaccccgtc attatcaacg aagccatcga agttaccaaa accttcggcg gtacggacgg
     3241 gcacaaattc gtcaacggca tcctcgacaa actcgctgcc caaatccgcc ccgacgagcc
     3301 caaacgccgt tgatgcatca caaatttaaa gaaagttcta tgtccttatt tctgctttct
     3361 ttgtttcgtc attcccacgc atgtgggaat ttggtttttt gaattttggg tatttcaaat
     3421 aaattctgaa acttcaatct cgtcattccc gcgcaggcgg gaatccggtt ttttgagttg
     3481 ctgccgtttt tgataaactc tggtcgcatc gaatttccgg attcctgcct tcgtgggaat
     3541 ggcgaaattt gaaaattgtg ggaatttatc ggaaataacc gaaacgtaac aaactcaaac
     3601 gccgttgacg tatcaatcag tcaacaacac aaatgccgtc tgaaaaacct ttcagacggc
     3661 attttgcttg accgttcaat caatactgta ccgtccagcc gatttcgccg cccgcgcgca
     3721 tcgggacaag ttcgccgtcg ccgtagggaa cacttgcggg gacggtttgg ctttgtttga
     3781 cgagggtgat cgtgccggcg ttttccggaa tgccgtaaaa ccttgcgccg tttttcgagg
     3841 caaaggcttc gagtttgtcc aacgcgcctg ctttttcaaa cacttcggcg taaagctcga
     3901 tggcggtcat cgcgctgaac ataccggcgc aaccgcaggc gttttcttta gcggatttgg
     3961 cgtgcggcgc ggaatctgtg ccgaggaaga atttgtgcgc cttctcgccg gtaacggcgg
     4021 caaccaatgc ctgacggtgg gtttcgcgct tgaggacggg caggcagaaa tgatgggggc
     4081 gcacgccgcc gaccaagagg tcgttgcggt tgagcaggag gtgttgcggg gtcacagagg
     4141 cggcaacgtt gtcgcctgct tccaaaacca ggcgggcggc ttcggcggtg gtaatgtgtt
     4201 caaacacgac tttaagattc ggcacttgcg ccaaaacggg tttcatcacg cgctcgataa
     4261 aggcggcttc gcggtcgaaa atgtcgattt cggggtcggt tacttcgccg tgaacgagga
     4321 acaggatgtc ctgttttgcc atttcttcca acacggggat gagcttgaat aggtcggtta
     4381 cgccggaatc ggaattggtc gtcgcgccgg cggggtagag tttgaaggcg acgatgccgg
     4441 cggctttggc ttcgcgcacc agctcgggcg tggcttggtc ggtcaggtaa agcgtcatca
     4501 gcggctcaaa cgcgctgcct tcgggcaaca ccgccataat gcgcgctttg taggcaagcg
     4561 cgtcggctac actgacgacg ggcggtttga ggttgggcat aatgacggcg cgccccattt
     4621 ggcgggcggt ataaggggca acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca
     4681 tatcgtcggg gcggataatg gtcagggttt gcatggttgt tcctttttag gttttggctg
     4741 cgtttgaata cccggtcgat tccatccgtt tgccggggga aagccggtta ttccgtccgg
     4801 tcggaaaccg gttccattgg atgaaaggca ttttgtccga ctgattgggt tgcatcaacg
     4861 gaatcttcaa acacagcctt tagattttga ttgtgcttgg gttttgcttc tttgccgacg
     4921 gcaaggcggt taacagccat tccggaacaa atcggcggcg atacgcccga aatgccgaca
     4981 ctgcacgccg gacggtttgt ttttgaaacg gcacgcggca catacgatat tcaaaaacaa
     5041 cgccgtcggg cgggacatcc gcatttcaga cggcatttat ttgtgcttga cgaccagcgt
     5101 gaacacgccg tcctgttcgg aagcatccaa cagcacatga ccggtttggc ggcaaaaagc
     5161 ctcaaaatcc cccggtgcgc cgccgtcggt tgccagaacg gtcaacacgt cgccctgctg
     5221 catttgcgcc aaagcctttt tcgcccgcaa aatcggcaaa gggcatttca gcccggttac
     5281 gtctaaagtt tcgctgttca tcgtttgtac cgtttaaaaa atgggaatcg gggggagatt
     5341 ataccccagt tgcttgcagc cccctttgcc aacccataaa ataacgccgt tttcaatttg
     5401 gagaaactta tgcgccgcac cgccctcctg ctgctcgcac tcaccgccgg cacgtccctt
     5461 gctgccggct tcaaccaaaa agacacgccg atcaacaccc gctaccgcga aacgcccgaa
     5521 gaagccgccg cccgcgaatt taaagaacat accgccaaac tgccgccgct gcccgatacg
     5581 cactcggacg gctggttcga catttatgtg gatgaaaatt acggcaaaca acccaaaatc
     5641 ctgctcgaca gcctgcaaat tatgcccgcg cccgacggca gcatccgcta cattctcaat
     5701 atccgctccg acaaaggcta cgacaacctg actgccgaag gcatattctg cgcccgcttc
     5761 tccatcaggt tcggcaacgg caaaccttcc tcctataaag tgttcggcta cggcgacacg
     5821 gtcaaccgcc gctggataca gccccgaaac gccgaatgga aaccgatagg cggtacatta
     5881 agccgcaacg acgcactgcg caccgtcctg tatcaggcat tctgcgaggg cggtgtaccc
     5941 gccgacacga aaggtttggt acaacgcctg aaagaacgtg ccggacgcta tgccccgtcg
     6001 ataaagccgc acaacaaata accagacaca aaaataccgt ctgaaacatc ggtttcagac
     6061 ggtattcgat tcatcagcca tcaagcggcg gaaactttgt cctgacggca caacagcgta
     6121 atcaaatcac tgatgcgccg cttcatatcg cggcggtcga caatctggtc gattgcgcct
     6181 ttttccagca ggaactcggc gcgttggaag ccttccggca gcgtttcgcg caccgtctgc
     6241 tcaatcacgc gcggaccggc aaaaccgatc agcgcgttcg gttcggcaag cacgacatcg
     6301 cccaaaaatg cgaagctggc ggatacgccg cccatagtcg gatcggtcaa caccgatata
     6361 aatggcaggc gtttttccgt cagcaaatgc agcgcggcac tggttttcgt catctgcatc
     6421 aatgagttca caccctcctg catacgcgca ccgccggaag ccgccacaca gacgaacgga
     6481 caattgtcgg caaccgcccg acggatacct tgtacgaatc gttcgcccac aaccgaaccc
     6541 atcgaaccgc cgataaagcg gaattcaaac gcggcaacga cgacgggcag accgttcatc
     6601 ttgcctttca ttaccaccaa agcatcatct tccccggtca gcttgcgtgc cgcactcaaa
     6661 cgatccggat attttttgct gtctttaaac ttcaaaagat cggtcggttt gacatttccg
     6721 gcaatctcct ctctaccatc ctcatccaaa agcagattca ggcgttgtcg tgccgacaac
     6781 ggattgtggt ggttgcattt cggacaaacc tgattgttct gctgcaactc ggtcgaataa
     6841 acggttgccg aacaagacgg acatttgtgc cacaaaccct cgggaacatt ggaagaaccg
     6901 tctttgctgc ggtttttgat tttgggtggc aggattttat ctaaccagct catggacaac
     6961 tcctaaaata tttagaatgc gtgacagaat cagcgcacgg catccttcaa ctcttttacc
     7021 aaagcaccga cggcggcagc ctcgttgcct gtattgtttt cgatttcttt cacaatccgg
     7081 ctgccgacaa taactgcgtc ggcaacccgg ccgattttgc gtgcactttc cgcattgctg
     7141 atgccgaaac cgacaccgat gggaatatcg atatactgat gcaaatactc tattttacgc
     7201 gaaacctcat ccgtatccaa acttgccgcg cccgttacgc ccttgagcga aacataatag
     7261 acaaatccgc ctgccagctc ggcaatggtt ttaatacggt cttccgtcgt cgtcggtgca
     7321 atcaggaaaa tacagtcaac cccgttgcct ttcagctcgc gatataaaga atcgatggtt
     7381 tcaatggggg aatccactgt caacacacca tccacccccg cctttgcggc ttcctgagca
     7441 aactcccgat aacccatctt atgtatggga ttcaaatacc ccatcaaaac aaccggcgtt
     7501 tgcgtgtcgg tttcacggaa ttttctgacg acatccaaga catcgcgcag cgaaatcccg
     7561 tttgccaacg cccgctccgc cgcacgctga ataaccggcc catccgccat cggatcggaa
     7621 aacggcacac ccaactccaa aatatccgca ccgtttgcaa ccatgccgtg catcaatgcc
     7681 aaagttgtcc gaatatcggg gtcgcccacc gtaatatagg gaatcaatgc ctttccgcca
     7741 tccaaagcgg caaaagcctg ccggattctg ctcatttgtc tcttccatcc tatcggcaaa
     7801 tatgccgtaa aacacaatca gtaaattttt cccagtataa catttgcggt acggataggc
     7861 gaatacggca aacagaaacc gcgcaaatca gagccgccgt ttgaacgagg cggaaataaa
     7921 cggcaagcat ctcggattat agtg
//
LOCUS       DAWKQJ010000086         7881 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000086 DAWKQJ010000000
VERSION     DAWKQJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.086"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..140)
                     /locus_tag="WJL20_001354"
     CDS             complement(<1..140)
                     /locus_tag="WJL20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7683507.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WJL20_001355"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WJL20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ7683508.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDEAVDPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQERTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WJL20_001356"
     CDS             1619..2668
                     /locus_tag="WJL20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7683509.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WJL20_001357"
     CDS             3356..4777
                     /locus_tag="WJL20_001357"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ7683510.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WJL20_001358"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WJL20_001358"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7683511.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5626..5970
                     /locus_tag="WJL20_001359"
     CDS             5626..5970
                     /locus_tag="WJL20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683512.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WJL20_001360"
     CDS             5981..6511
                     /locus_tag="WJL20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7683513.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WJL20_001361"
     CDS             complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WJL20_001361"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ7683514.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2067 a         1964 c         2035 g         1815 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaaaccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgtcaaa cgataccaag ccaaaaacgg cgaaaaaggc
      601 cacggctccg atcgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 acccttatcc gcgacctcga caccgacgaa atcgttgccg acctcaccta ccacggtcag
      721 cgcgtctgcc ttgccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaaacccg ctccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccga acttgggcgt ggtacgcatc gacgaaaacc acagtttcgt gatggccgac
     1021 atccccggcc tgattgaagg tgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacctatcgc gtaccggcct gctgttgcac gttgtcgatt tggcgccctt cgacgaagcc
     1141 gtcgatcccg ccgaagaagc ccttgccatc atcaacgaat tacgcaaata cgatgaagaa
     1201 ctctatggca aaccgcgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccaagaac gcaccgtcac cttcctcgaa gccgtcggct gggattaccc gaaaccggac
     1321 gaccgcttcc aattcgatat ggaaaccccg cgcctcttcc aaatcagcgc gctaacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgcttg
     1441 gaagccgaaa aagcagaagc ggagaaagcg gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatgcctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctc tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctaat ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcaaccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctta cccgccaaaa agaacccttt gcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtttgcg gcatgactgt ttacgactat tgccacttag gccatgcccg
     3481 tgtgatggtg gtgttcgaca tgattgcccg ttggttgcgt gagtgcggtt atccgctcac
     3541 ttatgtgcgc aacatcaccg acatcgacga caaaatcatc gcccgtgcgg ctgaaaacgg
     3601 cgaaaccatc ggcgaactga ccgcgcgttt cattcaggct atgcacgaag atgccgatgc
     3661 tttaggtgtg ttgcgtccgg atattgagcc gaaggcgacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa acggcgatgt
     3781 ttactacgcc gtgcgcgagt tttccgctta cggccaattg tcgggcaaat cgttggacga
     3841 cctgcgcgca ggcgagcgcg tggaggtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gcggccaaag caggcgagcc ggcatgggaa agcccgtggg gcaatggccg
     3961 tccgggttgg cacattgaat gctccgccat gagtgaaaac ctgttcggcg atactttcga
     4021 catccacggc ggcggtgcgg atttgcagtt tccgcaccac gaaaatgaaa tcgcccaaag
     4081 cgtcggtgcc agcggtcata cctgcggcca cgaccacgcg caaacccatc acggtcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttc atccgcgtgg atggcgaaaa
     4201 aatgtcgaaa tcgctgggca acttcttcac catccgcgaa gtattgaaac aatacgaccc
     4261 tgaagttgtg cgtttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gacgacgcga aaggcgcgtt gacccgtcta tacaccacct tgaaaaacac
     4381 gcctgccgcc gcgtttgatt tgtctgaaaa cgccaacgac tacacccgcc gcttctacgc
     4441 cgccatgaac gacgatttcg gtacggttga agcggttgcc gtgttgtttg agctggcagg
     4501 cgaagtgaac aaaaccaatg acgcacagct cgccggctgc ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg acccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat cgcccggcgg aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttaaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatacat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcatgggat gcacggccat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg atttgtgcgt tgcgatttcg
     5161 acaggttgag cgttatttcg ctttacttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 gacgcgacat tgtcgtttgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caatcagaaa aattcaggtt tcctgccgga agaacgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacgtg gtggagcaac cgcgggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgccg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttccgaccat gcgccgctgg tcatggagta tgactatgct gccgaataaa
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc agcatgggtt
     5701 gtcgctgcgt ttatcctgcc catattggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg acatgggcgt tggtgtggct gctgatctgc
     5821 atgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcgga cggcatttga acgcatacgt ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac actttccaac gcgtccgctg tccctgccgt gccgcattgg tggtgtccga
     6361 acacggggat gtgccgcgag attgggcgca aaaacaggcg gatacgtgga acgcgcgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcatatcgg gctgttttca ttgacgtgca ggcgcatcag gttcggataa
     6601 agtcgggctg ccgcataccg ccggaaatgg aatcctttat tttttacgtc ggcagtgccg
     6661 gcagggcgtt atccggcagg gttgcaagga aaatgccgtc tgaaacaggc ttcagacggc
     6721 atttccattc aaacagggct tacaggctgc ccaacacgat acgcaatacg cggcgcagcg
     6781 gctcggccgc gccccacaag agctggtcgc cgacggtgaa cgcgctgatg tattcgccgc
     6841 ccatgcccag tttgcggatg cgtccgacag ggacggacag cgtgccggta actttggcag
     6901 gagtcagctc gtggatgctg gcttcttttt cattggggat gactttcacc cagtcattcg
     6961 cgcctgccaa aatcgtttcg atttcggaaa caggcaggtc ttttttcaac ttcagagtga
     7021 tggcttggct gtggcagcgc atcgcgccga cgcggacgca caggccgtca atcacggttg
     7081 gattgtcgct gcggccgagg attttgttgg tttccacgcc gcctttccat tcttctttgg
     7141 actggccgtt gcccaaatcc acgtcaatcc acggaatcag gctgccggcg agcggtacgc
     7201 cgaagttggc tttcggatag tcttcgctgc gcaggaaatc ggatactttg cggtcgatgt
     7261 cgagaatcga gccggcagga tcggcaagtt cgtccgccac ttgggcgtga accgcgccca
     7321 taccgctgat gagttcgcgc atgtttttcg cacctgcgcc ggaagcggct tggtaggtca
     7381 tgctggttgc ccattcgacc aaatcgtttt ggaacaggcc gcccaaagcc atcagcatca
     7441 gggagacggt gcagttgccg ccgatgtagt ttttcacgcc gtttttgagg ccgttgtcga
     7501 taacgttgcg gttgacaggg tcgaggacga taatcgcgtc gtctttcatg cgcagagaag
     7561 atgccgcgtc aacccagtag ccgttccagc cgctgtcgcg cagggcttgg aagacggatt
     7621 tggtgtaatc gccgccttgg caggtaacga tgatgtccat tttggcgagt tcggcaacat
     7681 tgttggcatc taataatgtt ttagccgcct gaccgaaatc aggggctgcg ccgccgacgt
     7741 tggaagtggt aaagaaaaac gcttcaggaa tgtgggcgaa gtcgttttct tctttcatac
     7801 gctgcatcaa aaccgaaccg accataccgc gccagccgac gaaacctact ttcattgtgt
     7861 tgctccttga ggaaaaatcg t
//
LOCUS       DAWKQJ010000087         7866 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000087 DAWKQJ010000000
VERSION     DAWKQJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.087"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1337)
                     /locus_tag="WJL20_001362"
     CDS             complement(<1..1337)
                     /locus_tag="WJL20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ7683515.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1411..2751)
                     /locus_tag="WJL20_001363"
     CDS             complement(1411..2751)
                     /locus_tag="WJL20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ7683516.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLQLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            2894..3520
                     /locus_tag="WJL20_001364"
     CDS             2894..3520
                     /locus_tag="WJL20_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ7683517.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3584..5002)
                     /locus_tag="WJL20_001365"
     CDS             complement(3584..5002)
                     /locus_tag="WJL20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7683518.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            5329..6057
                     /locus_tag="WJL20_001366"
     CDS             5329..6057
                     /locus_tag="WJL20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ7683519.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6116..6604)
                     /locus_tag="WJL20_001367"
     CDS             complement(6116..6604)
                     /locus_tag="WJL20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ7683520.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFGWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7362..7826)
                     /locus_tag="WJL20_001368"
     CDS             complement(7362..7826)
                     /locus_tag="WJL20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ7683521.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2028 a         2123 c         1976 g         1739 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tagtggcgtc ccacggggct ttgcggctgt gcagctcgat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc ggcgagcagg
      241 gtcagggttt cagggtcggt gtgcaaggtt tggcggccgg agagtttgtc ggaaatggtg
      301 cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagtg cgccggttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcgag caggaaatcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cgcaccatga aaggataatc aattcatccc gatatttgtc gagaacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcgccg tggctgccga gaaactgttg cagccgttcg
     1081 atgccgtctg aacgattgtc cgtatggttt tccagccatt ccagcacgcc gcccgcgtct
     1141 tcgagtccgg gcgtgtcgta caggaaaacc agcgtgtctg cgccgtcgct gatggcggct
     1201 tcttcgacat gacgcgtggt cgatggggcg tttctgactt cgccgaaacc gctgtcgcgc
     1261 aaaagggtac gcaggagcga ggttttgccg gtgttggtgt gtccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacggtct tttttcagaa
     1381 tggcggctta acagaacatt tcaagtgagt ttattggtct ttcaaacggc cttcctgcgc
     1441 cgccctgtca ggctcaagcc acgccgcgcc gcattcggtc agcgcgttac gccaatgttc
     1501 cagcttttcc gaaaggtcgt ctgaaagccc ctgttccgcc aaaagctgca ccaccgcgcc
     1561 gccctgcgcc gcttccgaaa gtcggacgat ctgccgcaac acgccgcggt cgggcacagt
     1621 ttgggcgcgc acgccgataa gcagttgcgc cggtttctgc ttcagctctg tctccagcgc
     1681 ggcaacctgt tcccgattgg cggcaacgcc cttatccagc cattcctgcg ccagcctgcc
     1741 ctcgaaccat tcgccgtcct gccattcggt ctccagcatg accgcccatt tcggcgcatc
     1801 gttcaagacg attttcggcg aaacggcgga cacggtttcc cgacgcgtat ccgcatcggt
     1861 gattttgttc tgccagcggc ggatgaccgc ctgataatag ggtttttcca aatccaatcc
     1921 gttttcgctt gttttcaaaa ggattttgca taccgcccaa gccaagaggc gcggcaggat
     1981 gccgtagcag gcgatactgc cgaccagcag ccccgaccaa gcccgcgcat cggcaatatt
     2041 gccgttcaga cggccttcga tgaccgcccg cgcatcaggc acgggaaaac ccagtttcgc
     2101 aggcagccat gccaacattt ccaccagccg taccgaagac gaatcgccca acagcgtgct
     2161 ttcccagttg aacgtatatt gccgcaccaa aagcagcaac aataccgaca ccagcattcc
     2221 gagcagcgtg cagagccaca ggctgtgcga cgttgcgcct attttccaac gtaccgaagg
     2281 ttgccgccac tcgtccgcat acagctgcaa caccgcctga ttgacagggt ctttgccccg
     2341 aaaccacgtc gccggactgc tgaaaaaacg ccccactttc acgcgcagga acaacattgc
     2401 caaccatact gctaacatca gcgtattcat gcccaacacg cccgccaaaa tcaggaagaa
     2461 attcagcccc tgattgtcca tcagaagata agtgcctgaa aatccggcgg taaacatcat
     2521 cgatgccgcc accacccaca accagaacga ccccacacgc acacgttcca acgtgtcccg
     2581 cagcatacgg ttcctgtcga tcatctccgc ccgacggatg attttttcct ccgtactgcc
     2641 gtccacgcgg cgcaaagcct ccgtcgcctg cacgggatcg ccgctgaaaa taaagccgcc
     2701 ttcttccaaa atacggacca gctcaaccag ttttcgggat ggattcaaca taaaatgccg
     2761 tctgaaaata aaaaacagat tttaacacac gaattttcaa gaatattcac agtgtaggca
     2821 aagagtaaat cccacacaga ggcaaaagta tcggcgtaaa ctgactgcct ctactttccc
     2881 gaaagattgt gcgatgtata caggcgaacg cttcaatact tacagccatt tgagcggttt
     2941 gattctggcg gcggcaggtt tggtgctgat gctgctgaaa accataggac acggggacgg
     3001 ctaccgtatc ttcagcgtat cggtttacgg catcagcctt cttctgctct atttgagttc
     3061 ctcgctgtac cacggaattg cagccggaaa actgaaaagc attttgaaaa aaaccgacca
     3121 ctgcatgatt tatgtcctga ttgccggaag ctacacaccg tttgcactgg tttctttgag
     3181 aaacgggccg ggctggacgg tattttcact gtcctggctg ctggcggctg caggaatcgc
     3241 acaagaactc accatcggac ggaaaagcga aaaacgtctg ctgtctattg tgatttatgt
     3301 cgtcatgggt tggatggtct tggcggtaat gaaatccctg acagcctcac tcccgtcggc
     3361 aggactggct tggctggcgg caggcggtat gctgtacagc gtcggcattt actggtttgt
     3421 aaacgatgaa aaaatccgac acgggcacgg aatctggcat ctgttcgtat tgggcggcag
     3481 catcacccaa tttgtcagtg tgtacggtta tgtaatctga atgccgtctg aaaaacaaaa
     3541 cctcccgatc ctgaagattg ggaggttttc tgtttgccgg acatcagccc ttgtcgtgga
     3601 actcgtggaa ttcatacgga taagacaaat cccgacccgc ttttttctgt gccaaataat
     3661 catcataaat ggcacggatt tccttacgca acaaaaacag ggctatcaag ttcgggataa
     3721 ccataaagcc gttgaacata tcggacaggc tccaaaccaa atcgactttg ccgagcgtac
     3781 ccaaaacgat ggcaagcaga accaatgcgc gatagatgcc caagtgtctt cccctgaaaa
     3841 gaaaacggat gttggactca ccgaaataat accagccgat aatggtggtg aaggcaaaga
     3901 aggtcagaca cacggcaagc aattgcgaac cgaagcccgg aaatgccttg ttaaaggcaa
     3961 attgagtaac cgccgcgccc tgttcgcccg aaaggttggc atcggtcagc aggataatca
     4021 atgcagttgc cgtacatacc aaaatcgtat cgataaacac accgacaaat gccgccatac
     4081 cttgctgcac aggatgcttc acatccgcag tcgcgtgggc gtgcggggta gaacccatcc
     4141 ccgcttcgtt ggaaaacaga ccgcgcgcca cgccgaaacg tatcgcttcg cgcataccga
     4201 tacccgccgc accgcccaaa acggcttcag gattgaaggc ggcggtaaag atgtggttga
     4261 acatcggcac aatatagtcg gaaaattcaa acaggataac gacggcgcac aaaatataaa
     4321 caaccgccat aaacggcacg acaaactggg caatattggc aatacggttc acgccgccaa
     4381 tcacaaccat gcccgcaagg acggcaagca caataccgac tgccaaagaa ggcacatcaa
     4441 atgcaatggt aacggcagaa gcaatggagt ttgcctgtgt cgcattaccg ataaagccca
     4501 atgcgataat caacgcaatg gagaaaaagc ccgacaggaa acgcgccgcg cccctgccga
     4561 ttttcggagt cagaccgtgc gtaatataaa acgccggccc accgatgtat ttgccgtggc
     4621 tgacgacgcg gtatttctgc gccagcagtg cctccgcaaa aatcgtggac atccccaaaa
     4681 cggcagaaac ccacatccaa aaaatcgcgc ccggcccgcc tgcggtgatg gcggtcgcca
     4741 cgccggcgac gttgcccgta ccgatttgcg cggatatggc aaccgccaac gcctgaaact
     4801 gcgataaaga cttatcgtct ttatcgcctt tggcaaacaa gccgccgaat acagatttga
     4861 atcccgcacc cagcttggta atctgcggcg caccgagata cagcgtaaaa aacaggccga
     4921 tacccaaaag cgcgtaaatc agcaggtagt cccaaaggaa ccgattgact gtacccacca
     4981 gaacagacaa tatattttcc ataaaataaa ccttatctta caattaaaat gactgccttc
     5041 caaaagacat tccaataagg aaacacggcg agcagaccgt atttgccgca acagatgcct
     5101 taaattgtca acaatcgggg agaagctgcg ccatcatacc gtaaaatatc gttaattaaa
     5161 acatttcttt atttttaagc ggaaagcgga ggaaatcgct ttcagacggc atcgacaacg
     5221 gcacggcata aaacaggata ttttgggtac ttgcaactta tgttaaaatg ccgaccgtaa
     5281 aaaatctgac aaaaacagat taattatttg aaataagaaa ggaaatttat ggcaggccat
     5341 agcaagtggg cgaatatcca gcataaaaaa gcccgtcagg atgccaaacg cggcaaaatc
     5401 ttcacccgtt taatcaaaga aatcaccgtt gcggcgcgta tgggcggcgg cgatcccggt
     5461 gcaaatccgc gcctgcgcct ggctttggaa aaagcagccg aaaacaatat gcccaaagac
     5521 aatgtgcaac gcgccatcga caaaggcacg ggcaacttgg aaggcgtgga atacatcgaa
     5581 ttgcgctacg aaggctacgg catcggcggc gcggctttga tggtggactg cctgaccgac
     5641 aacaaaaccc gcaccgttgc ggacgtacgc cacgcgttta ccaaaaacgg cggcaacttg
     5701 ggtaccgacg gctgcgtggc gttcaacttc gtgcatcagg gctatttggt attcgaaccc
     5761 ggcgttgacg aagacgcgct gatggaagcg gctttggaag ccggtgcgga agacgtggtt
     5821 accaacgacg acggttccat cgaagtcatt accgcgccaa acgattgggc gggcgtaaaa
     5881 tccgctttgg aggcggcagg ttacaaatcc gttgacggcg acgttacgat gcgcgcccaa
     5941 aacgaaaccg aactctccgg cgacgatgcc gtcaaaatgc aaaaactgat tgacgcgctg
     6001 gaagacttgg acgacgtgca agacgtttac acttccgccg tattgaattt ggactgatac
     6061 gcagcacagc agacatacaa taaaatgccg tctgaacctt tcagacggca ttgatttatt
     6121 tagcccttgc ccacgcccac caaaccgtca ggacaaccgc cagaaaatac gccgcgctcc
     6181 aaacaggcaa agcaaccccc agcagataat ccggttcggc acaatttccg aacccgcgca
     6241 cgacaggctc gaaccaacca aacaaaggcc agcccttcaa tcggaacgtc cacggcgcac
     6301 cgcacgaagg agccgtaccc ggcggcagcg actgcaacca caactgatat gccgcaacag
     6361 aaatacccgt aacggccggg atgctgataa agacagcacc gaacaaaccg cccgcccttc
     6421 ttcttggtct gcacatcagg acaattgccg tacacaatgc ggttgccaaa acgcataacc
     6481 gctgactgat acacaaaacg caaggctcca tacccaaaac atactgtgcc gccaaagaac
     6541 cggcaaatgc acagaccgaa acggcaaaca gcagccaaac ggcttttcta aataacgggg
     6601 tcattttctc aacacaccaa tcaaaatacc gatatgccga ttttgctgga tatatcccga
     6661 gatggcaagg gacgaatcga caccggctca ggtgcggacg atttcggttt tcccgatata
     6721 cacaagacct gacaagggac aaaacggcga tttgcccgca caatgcccgc aaaaaaatac
     6781 atccggagga tttgaatttc ggcaaattta gcgaatcaaa agtttatttc aatgaaatca
     6841 tatgattttt ttgaataagc ggattgacag gtttttgaag gaatttgtta ccggatagcc
     6901 atcgggcaag ttttttgcaa aatttgaatc ggtcgggtaa attttcaaaa aataattgac
     6961 agcggataag aaacggcgga taattctcgc cgtcgagttg cttgatgcag cttgattttt
     7021 ctcctctatt tctcctttgt agacttggca cacattcaac cggatgtgtg cattttttta
     7081 tctccgcttt ttttgaaatt taattacttt tattgtttga aattccattc tttaagaaat
     7141 ttaacaagaa aaacgcttgc tttttgaccg gaaaagctgc ataattcatt ccgttagctg
     7201 gttcgagtag tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt
     7261 caaccggatg tgtgcatttt tttatctgaa gcaacaagcc tctgtgcgtg atgttgttat
     7321 gtttcattta ggtgtcaaac cgcatagcca gcctgaaata ttcaatccaa atccaaaacc
     7381 ggattttctt tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt
     7441 tgcaatagga tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta
     7501 atcagatagt cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc
     7561 acttcttttt tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca
     7621 aaaaccaata gtggtttgcc caacagatgg ggattgagat gggcgtgata gccggaaata
     7681 taatgttccc gctccaaacg gcgcaccctc tctgtaacgg gcgtggtgga caagcctacc
     7741 ttctcggcaa gctccgtcat cgggatgcgg gcattctgtt ggaggatctt aagaatgcgg
     7801 aaatcgattt tatctagttc tttcatttgg attaccttgt atttattatt gattttaaca
     7861 aataga
//
LOCUS       DAWKQJ010000088         7846 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000088 DAWKQJ010000000
VERSION     DAWKQJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.088"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..1886)
                     /locus_tag="WJL20_001369"
     CDS             complement(90..1886)
                     /locus_tag="WJL20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ7683522.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVCLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLIDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2344..3597
                     /locus_tag="WJL20_001370"
     CDS             2344..3597
                     /locus_tag="WJL20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7683523.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3603..3755
                     /locus_tag="WJL20_001371"
     CDS             3603..3755
                     /locus_tag="WJL20_001371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683524.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WJL20_001372"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WJL20_001372"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ7683525.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WJL20_001373"
     CDS             complement(5340..5528)
                     /locus_tag="WJL20_001373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ7683526.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(5532..6409)
                     /locus_tag="WJL20_001374"
                     /pseudo
     CDS             complement(5532..6409)
                     /locus_tag="WJL20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(6439..7566)
                     /locus_tag="WJL20_001375"
     CDS             complement(6439..7566)
                     /locus_tag="WJL20_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7683527.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1704 a         1993 c         2205 g         1944 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtctat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttgatt
     1021 ccctcgataa ggcacacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggttcgataa gcaacgcgcc gccgatttgc gcgagtttgt cggcaacttg ggcgtaaggc
     1141 tcgaccgtga tgcctgcggt ttgcagcgcg gcggcggctt cggcattcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcaatagat aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaagcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatg acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcggg gatagcggga
     1441 cggcttgtcc acacttggtc aagcagatcg tccgggtgtt cgatgcggat gtttttggcg
     1501 gtcagggatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tgtacggcgg cacttgaccg
     1621 cttttttgca gctcgatgcc gctgccggaa agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacacccgc ttcatcggtg gtcaggacaa atgtgccgac cgagccggta
     1741 aagcccgaca attcgcggcg cgcctgccag tgtgcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcgtccaag ccttgcgccc tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt tcatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg cgctttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cggttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggaccgggg
     2401 attatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagcccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggtgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtac agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgctcctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gctttaccgt tttgttccta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gccgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata cgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agctttgcca ctattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccagggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctgcg
     4201 ccctctgccg gacaaacata aaggcttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgtcgatttg attgccaacg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccaccaca atgccttgga
     4441 cattccgctc tacctgcgca tcgcccccga gctgtatctg aaacgcttgg ttgtcggcgg
     4501 tttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggtatgt ctgtgcgcca
     4561 taaccctgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcaaatggcg gaagacatca tccgcaacgc ttcccgcgcg gtaaacggca cggcaaacat
     4681 cacttacaac ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccattctcga
     4741 tgccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggagtggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgcctccg tctccgggca tcggcagcct
     4861 gcaacttgcc ctgtttgaag gttgcgccga gggcaaattg tggaacccga ctttcattat
     4921 cgactaccct gtcgaagtgt ccccgttggc acgcgcatcc gacagcaaac cgggcttgac
     4981 cgaacgcttc gagcttttcg ttgtcggccg cgaactggca aacggctact ccgagttgaa
     5041 cgatccggaa gaccaagccg aacgcttcaa ggcacaagtg gcgcaaaaag acgcgggcga
     5101 cgacgaagcg atgcactacg atgccgacta catccgcgcg atggaattcg gcctgccgcc
     5161 gaccggcggc tgcggcatcg gcatcgaccg cttggtcatg ttgctgaccg attcgcaaac
     5221 catccgcgac gtgattctgt ttccgcagat gcgccccgag tagtcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcgcttt ctaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttcttgcctg tgcggcggac
     5581 ataaaccgtt ttttgactcc agcaccacac ctaaaatcgt gctgccttcg ggcaggtcaa
     5641 aggcttcaga cggcctgccc gtctgataaa tcagcatcct gcccgcaata tcgataacaa
     5701 ggtgcgcgac gaccagccgc ttgtcggaca gttcaatcag cacgatgtcc tcgtccgcag
     5761 ggcgcatacg cggctcgacc accaccaaat cccctttttc gataaccggc gacatggacg
     5821 tgtcgggcat ggcaacggca aacgtgtact gcgagggggc gaccaccgaa gcaaacaagt
     5881 cgaaactgcc tgtgagtttg cctgtacggg cgaaagccgc cgcctgacgg tctgacaaca
     5941 gggggcgtgg tgtgagcctc atcgacagtg ctttgccgtc ggcatcgctg tggttgtgcg
     6001 tatgcgtatg atggttgttg atggtcgtat cgccttggac atcgttaagg actatgctgt
     6061 ttgaacctgc cacggatttg gtcatctcat tgtaggcttg cgtcgtgatg cgctcttgct
     6121 tggtcatacc tgttacagcc tgtttcagca tttctaacgc tatgtgattc ttcctaaggg
     6181 tcgctatacc ggcttttgca gcttgctcat taccacaaga ggggcaaccc cagctacttc
     6241 ctatgaacat attggagtac ctcgaacagg tttggtttcc atgtttaggg cagttgatta
     6301 cacacggctt ggctgtaccc tcaaactcga ccaacttaat gccacgatga ccgaaacgtt
     6361 cctcaacctc agcagcagct tcttcaaatg tttttgacct tcttgccata ttgacctccg
     6421 gattattgat tattttggct atgctttaac gagggattgg aatgaggtaa agctgttgcc
     6481 ctctccccgc aatagttcca ttaggcgcgg atgaatgaat agtttgtccc tgccgatggc
     6541 gatttcttgc agcacaccta tgtctgaaag ctctttcagg tacttagaag ccgtctgccg
     6601 tttggctatc cctgccgctt ctaggttggc aatgcgtgta tatggctgct caaacagaag
     6661 atttaccagt tcgtgcgtgt agattccttg tgcgtgtgtc cgtatgtgtt gacgtgtctg
     6721 ctcaaacagg cggcgtatcg catctatttt cgctaccgtc caatcggcgg tgtcggctac
     6781 gccgtctaag atgtagatta tccagctttc ccagtcctgc cgttcggtta cgcctaaaag
     6841 caggcggtaa tagtccgccc tgttttcgat aatatagcgg ctcaaataca aaataggcaa
     6901 atccaaaagc cctttttcaa tcaatagcag gctgttcaat atgcgccccg tccgcccgtt
     6961 gccgtccgta aacggatgga tggcttcaaa ttggtaatgt gccgccgcca tgatgataag
     7021 cgggtctaaa tcgccgcttt cgtgaataaa ccgctcccaa tttgccagct tgccgcgtat
     7081 ggtttcttct ccttcgggcg gggtatagac aacatttccg ctgttgcctc cttttagggc
     7141 tgtgccgcct gttttgcgga tggccatttc gtaggggtgc ttgatggcgt tgcagaccat
     7201 gatggcggtt tgtgtgcata aagggcggct cgtcagtgat tcatagcctg caaacagggc
     7261 ggtgcggtat tgcagggctt ctttcgtggc agggtcttgc cgttccgtat ccatttgcag
     7321 ggattgaaac agcttgtccg tggtggttac gatgttttca atttccgaac ttgcacgggc
     7381 ttccataaca ggaagggtgt taatcagcat ggcttgattc ggtatcaatt ctgccgcctg
     7441 ctttaaacgg gcaagggatg cacgggcggc tatacaacgt ttcaggatgg ttttgctttc
     7501 aatatcctgt tttggcggca ggggtggtaa atcgttatag ggaatattgg gtttccagtt
     7561 gctcatattt aaaatttcgg aaaatttaaa gatgtttcca gtatatgttt acgccgtgta
     7621 tatatcaagg atatatgttt aaaaatttgg cttttgtaaa tatatgggcg gtaaaaccgc
     7681 attattttat gttgttacct attttacacc ttgtctgcac acgatttcca aacaacctaa
     7741 aaagaaacct gccgaaaggc aggtttaaag aggtcatttt aaatgtgaac taaagcttac
     7801 accctcgtag ggtatcgtgt atatcagacg tacttaatat acacta
//
LOCUS       DAWKQJ010000089         7747 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000089 DAWKQJ010000000
VERSION     DAWKQJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.089"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            79..1254
                     /gene="ctrA"
                     /locus_tag="WJL20_001376"
     CDS             79..1254
                     /gene="ctrA"
                     /locus_tag="WJL20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ7683528.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            1269..2432
                     /gene="ctrB"
                     /locus_tag="WJL20_001377"
     CDS             1269..2432
                     /gene="ctrB"
                     /locus_tag="WJL20_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ7683529.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            2432..3229
                     /locus_tag="WJL20_001378"
     CDS             2432..3229
                     /locus_tag="WJL20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7683530.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            3226..3876
                     /locus_tag="WJL20_001379"
     CDS             3226..3876
                     /locus_tag="WJL20_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683531.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            3940..6213
                     /locus_tag="WJL20_001380"
     CDS             3940..6213
                     /locus_tag="WJL20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ7683532.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            6244..7246
                     /gene="dcm"
                     /locus_tag="WJL20_001381"
                     /pseudo
     CDS             6244..7246
                     /gene="dcm"
                     /locus_tag="WJL20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7239..7583
                     /locus_tag="WJL20_001382"
     CDS             7239..7583
                     /locus_tag="WJL20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ7683533.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            7626..>7747
                     /locus_tag="WJL20_001383"
     CDS             7626..>7747
                     /locus_tag="WJL20_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7683534.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKAMVERMLT"
BASE COUNT         1977 a         1711 c         2024 g         2035 t
ORIGIN      
        1 tatactctac gtactcataa atttatttcg attaagtgct ataattaggc catttataat
       61 tatattagga tttggcttgt gtttaaagtg aaattttata ttcgtcacgc agtattatta
      121 ttgtgtggaa gtttaattgt aggatgctct gcgattcctt catcaggccc cagcgcaaaa
      181 aaaattgtct ctttagggca acaatctgaa gttcaaattc ctgaagtgga gctgattgat
      241 gtgaatcata cggttgctca gttattatat aaggctcaga taaatcagtc attcactcag
      301 tttggcgatg gttatgcttc ggctggtacg ctaaatattg gtgatgtatt ggatattatg
      361 atttgggaag cgccgccggc agtattgttt ggtggtggcc tttcttcgat gggctcgggt
      421 agtgcgcatc aaactaagtt gccagagcag ttggtcacgg cacgtggtac ggtttctgtg
      481 ccgtttgttg gcgatatttc ggtggtcggt aaaacgcctg gtcaggttca ggaaattatt
      541 aaaggccgcc tgaaaaaaat ggccaatcag ccacaagtga tggtgcgttt ggtgcagaat
      601 aatgcggcga atgtgtcggt gattcgtgct gggaatagtg tgcgtatgcc gctgacggca
      661 gccggtgagc gtgtgttgga tgcggtggct gcggtaggtg gttcaacggc aaatgtgcag
      721 gatacgaatg tgcagctgac acgtggcaat gtagtacgaa ctgttgcctt ggaagattta
      781 gttgcaaatc cgcgacaaaa tattttgctg cgtcgcggtg atgtggttac catgattacc
      841 aatccctata cctttacgtc tatgggtgcg gtggggagaa cacaagaaat cggtttttca
      901 gccagaggct tatcgctttc tgaagccatt ggccgtatgg gcggtttgca agatcgccgt
      961 tctgatgcgc gtggtgtgtt tgtgttccgc tatacgccat tggtggaatt gccggcagaa
     1021 cgtcaggata aatggattgc tcaaggttat ggcagtgagg cagagattcc aacggtatat
     1081 cgtgtgaata tggctgatgc gcattcgcta ttttctatgc agcgctttcc tgtgaagaat
     1141 aaagatgtat tgtatgtgtc gaatgcgccg ttggctgaag tgcagaaatt tttgtcgttt
     1201 gtgttctcgc cggttaccag tggcgcgaac agtattaata atttaactaa ttaatgtgag
     1261 taattaagat gtctgagcaa cttcctgtgg cagttgccac tgaaaccaaa gccgagcgta
     1321 aaaagccgaa aaagaaaagt tggattaaaa agctaagccc tttattttgg gtaacggtga
     1381 ttatccctac ggtaatttcg ttggtgtatt tcggcttctt cgcttccgat cgttttacgt
     1441 cgcaatcgag ctttgtggtg cgctcgccta aaagccaatc ttctctcaat ggcctgggtg
     1501 ccattttgca gggcacaggt tttgcccgtg cgcaagatga tatttacacg gttggggagt
     1561 atatgcgttc gcgctcgtct ttggatgaac tgcgtaaaat cttgccggtg cgtgagtttt
     1621 atgaaaccaa aggtgatgcg ttcagccgct ttaatgggtt tgggttccgt ggcgaggaag
     1681 aggcttttta tcaatactat aaaaatcagg tgatgatcaa ttttgatacg gtttcgggta
     1741 tttccacgtt gaatgtaact tcctttgatg cgctggaatc taagaaaatc aatgaggctt
     1801 tgttaaaaca aggtgaagca ttgattaacc agttgaacga tcgtgcacgt gctgatacgg
     1861 tgcgctatgc ggaagaagta gtgaaaacgg cggcagagcg ggtaaaggaa gcctctcaga
     1921 atctgacgga ttaccggatt gccaatggcg tttttgattt gaaagcgcaa tcggaagtgc
     1981 aaatggggtt ggtttccaag ctgcaagatg aattgattgt gattcaaacc cagctggatc
     2041 aggtgaaagc agtcactccg gagaatccgc agattccggg tttgcaggcg cgtgagcaga
     2101 gcttgcgtaa agaaattgac caacagttac gtgccatttc gggcggtggg cattcttcgt
     2161 tgtctaatca ggctgccgaa tatcagcgtg tgtatttgga aaaccagttg gcagagcagc
     2221 agttggcagc cgccatgact tctttggaaa gtgccaaggt tgaagcagac cgtcagcagc
     2281 tttatttgga agtgatctcg caaccgagcc tgccggattt ggcacatgag cctaaacggt
     2341 tatacaacat tgttgccact ctgattatcg gcttgatggt ttatggtatt ttgagcctgt
     2401 tgactgccag cattcgtgag cataaaaact gatgaaagcc ttgcataaaa catcattttg
     2461 ggaatcttta gccattcaaa ggcgcgtaat cggtgcgctt ttgatgcggg aaattatcac
     2521 ccgttacggt cgcaataata ttggcttttt atggctgttt gttgagccgt tgctgatgac
     2581 attcgttatc gtcttgatgt ggaaattttt aaaggcagac cgatattcaa ctttgaatat
     2641 tgtcgcattt gcgattactg gctatccgat gttgatgatg tggcgtaatg cctcaaaacg
     2701 ggcagttggg tcgatttctt caaatgccag cttgctttat caccgcaatg taagagtttt
     2761 ggataccatc ttggcgcgca tgattttgga aattgctggt gcaaccattg cgcagattgt
     2821 gattatggcg gtattgattg cgattggctg gattgaaatg ccggcagata tgttttatat
     2881 gctgatggct tggcttttga tggctttttt tgcgattggt ttgggtttgg tgatttgttc
     2941 gattgccttt aatttcgagc cgtttggcaa gatttggggc acattgactt ttgtgatgat
     3001 gccgttatcg ggtgcattct tttttgtaca taatttgcct ccgaaagtgc aagagtatgc
     3061 attgatgatc ccgatggtgc atggcacaga aatgttccgt gccggatatt ttggcagcga
     3121 tgtaattacc tatgaaaatc cttggtatat cgtattgtgc aatctggtgt tgttgttgtt
     3181 tggcttggcg atggtcagta aattcagtaa aggagtcgag ccgcaatgat ttcagttgaa
     3241 cacgtttcca aacgctatct gacccgccaa ggttggcgga cagtcttgca cgatattagc
     3301 ttcaaaatgg agaagggcga gaaaatcggt attctcggcc gcaacggtgc aggtaaatcg
     3361 acgctcatcc gtttgatcag tggcgttgag ccgccgacca cgggtgaaat caagcggaca
     3421 atgagtattt cttggccttt ggcattctcc ggtgcgtttc aaggcagtct gaccggtatg
     3481 gacaatttgc gtttcatctg ccggatttac aatgtcgata tcgattatgt gaagaacttt
     3541 accgaagagt tttccgagtt ggggcaatat ctttacgagc cggtcaagcg ttattcttca
     3601 ggtatgaagg cgcgtttggc atttgctttg tcgttggcgg tagagtttga ttgctatttg
     3661 attgacgaag tgattgcagt tggtgactcg cgttttgccg ataaatgtaa gtacgagttg
     3721 tttgaaaagc gcaaagaccg ttccatcatc ttggtgtcgc acagccacag cgccatgaag
     3781 caatattgcg ataatgcgat ggtgctggaa aaagggcata tgtaccagtt tgaagatatg
     3841 gacaaagcct acgaatatta taattcgctg ccttaaagcg attgttttta aatcaggccg
     3901 tctgaaattt cagacggcct gtccgttgga attctattga tgaacattac tcaaattctt
     3961 tcccaagaac tctccgcgac tgccgcgcaa atcaccgccg ccgtcgagct tttggacgac
     4021 ggcgcgaccg tgccgtttat cgcccgctac cgcaaggaag cgacgggcgg gttggacgat
     4081 acgcagttgc gccggcttgc cgagcggctg caatatctgc gcgagttgga agagcgcaaa
     4141 gccgttgttt taaaaagcat tgaagagcaa ggcaagcttt cagacgacct cagggcgcaa
     4201 atcgaagccg ccgataacaa aaccgcgctg gaagacctgt atctgcccta caaacccaaa
     4261 cgccgcacca aagcgcaaat cgcgcgcgaa cacggtttgc agccgctggc ggacgtgttg
     4321 cttgccgagc agtcgcagga cgtggaagcc gccgcacaag gctacctgaa cgaaaacgtc
     4381 cccgatgcca aagccgcgtt ggacggcgcg cgtgcgattc tgatggagca gtttgccgaa
     4441 gacgcggaac tcatcggcac gctgcgcgac aagctgtgga acgaagccga aatccacgcg
     4501 caagttgttg gaggcaaaga aaccgaaggc gaaaaattca gcgattattt tgaccaccgc
     4561 gaacctattc gtgcgatgcc cagccatcgt gcgctagcgg ttttgcgcgg acgcaacgaa
     4621 ggcgtgttga acatcgcgct caaatatcag cccgacgaca cgccgattac gcagcaaagc
     4681 gaatacgagc aaatcatcgc ccgccgcttc aaagtttcag acggccacaa atggctgcgc
     4741 gacaccgtgc gcctgacttg gcgcgcgaaa atctttttgt cgttggaact tgaagctctc
     4801 aatcgtttga aagaagccgc cgacaccgac gcgattaccg tgttcgcccg caatctcaaa
     4861 gacttgctgc ttgctgcgcc cgccggacgg ctgaccacgc tgggtctcga ccccggctac
     4921 cgcaacggcg tgaaatgcgc cgtggtggac gacaccggca agctgctgga taccgtcatc
     4981 gtctatttgc atcaagaaaa caatatgttg gcaacgctgt cgcgcctgat taaacagcac
     5041 ggcgtgaagc tcatcgccat cggtaacggc accgccagcc gtgaaaccga caaaatcgcg
     5101 ggcgaactgg tgcgtgaaat gtcgaaaatg gggctgcata aaatcgtcgt gtccgaagcc
     5161 ggcgcgtcga tttattccgc gtccgaactg gcggcgcgcg agttccccga cttggacgtt
     5221 tccctgcgcg gcgcggtgtc catcgcccgc aggctgcaag acccgcttgc cgagttggtc
     5281 aaaatcgacc ctaaatccat cggcgtgggc cagtatcagc acgatgtgaa ccaaaaccag
     5341 ctcgccaaat cgctggacgc agtggtcgaa gactgcgtga acgccgtcgg cgtggacgtg
     5401 aataccgcct ccgccccgct cttggcgcgg atttccggct tgaatcaaac ccttgcccaa
     5461 aacatcgttg cctaccgcga tgaaaacggc gcgttcgaca gccgcaaaaa attgctgaaa
     5521 gtaccgcgtt tgggcgaaaa aaccttcgag caggcggcag gctttttgcg gattaacggc
     5581 ggtaaagagc cgttggacgc gagcgccgtc caccccgaag cctatcccgt cgtcgccaaa
     5641 atgctggcgc aacaaggcat tagcgccgcc gaactcatcg gcaaccgcga gcgcgtgaag
     5701 caaatcaaag cgtccgactt caccgacgaa cgcttcggcc tgccgaccat tttggacatc
     5761 ctgtccgaac tggaaaaacc cggccgtgat ccgcgcggcg agtttcagac ggcatcgttt
     5821 gccgaaggta tccacgaaat cagcgacttg caagtcggta tgatactcga aggcgtggtt
     5881 tccaacgtcg ccaacttcgg cgcgttcgtg gacatcggcg tccatcagga cggcttggtg
     5941 cacatctccg ccctgtccaa taaattcgtc caagacccgc gcgaagtcgt gaaagccggc
     6001 gatgtggtga aagtgaaggt gctggaagtc gatgctgcac gtaaacgcat cgcgctgacc
     6061 atgcgcttgg atgacgaacc gggcggcgca aaacataaaa tgccgtctga aaaccgcagc
     6121 cgcgaacgga cagccggccg caaaccccaa cgcaacgacc gcgccccagc caattcggcg
     6181 atggcggatg cgtttgcgaa gctgaagcgg taaaataatc gaagagttta tggattttga
     6241 cttatgcaca caccacttac ctatattgac cttttctcag gagcaggagg cctatccttg
     6301 ggttttgaac aagccggatt ccaacaattg ctttctgttg aaatggagtc tgattattgt
     6361 cagacttacc gtaccaactt cccccatcat caattactgc aaaaagattt aaccacacta
     6421 accgaacaag atttaatcaa ttgtcttaac ggacaagcag ttgatttgat tattggagga
     6481 ccaccttgtc aaggttttag tatggcagga aagattggac ggacatttac agatgaccca
     6541 cgcaaccatt tatttaaaga gtttgtccga atagttaaaa ttgtccaacc atattttttt
     6601 gttatggaaa atgtagcgcg actctataca cacaattcag gtaaaacacg tattgagatt
     6661 attcaagcat ttcagaatat cggttattcg gtggaatgta agatactgag tgcagccgat
     6721 ttcggtgttc ctcagatacg tagccgagtg atatttatcg ggaggaggga taaaggcaaa
     6781 atttcctttc ccgaaccttt gcagatttcc catcagactg ttggatcagc aataggacat
     6841 tttccaaaac tggctgctgg cgaaagcaat ccacacgttg caaatcatga agctatgaat
     6901 cattcggcac aaatgttaga aaaaatggca tttgttaaaa atggaggtaa ccgtaacgat
     6961 attcctgaac cattacgtcc gaaaacaggt gatatccgta aatacatccg ttacaacagc
     7021 aacaaaacca gccgtttgta ttacaggaga tatgcgcaaa gtttttcact atgaacagaa
     7081 tcgggcgtta accgttcgtg aattagctgc cttacaatct ttccctgata attttatttt
     7141 ttgcggcagc aaaattgccc agcagcagca ggttggtaac gccgtaccgc ctttattggc
     7201 aaaagctatt gctgaaagta ttttaaaaat gagtgaaaat gaataagcaa tatccgaaaa
     7261 ttaactatat cggtaataaa gagaaaatag cttcctggat ttgtgaccag cttccgtctg
     7321 atgtagatac agttgcagat gtatttagtg gaggctgttc ctttgcctac gaagccaaaa
     7381 aacgcggcta tcgtgtgatt actaacgata ttttggcaat taattaccaa attgctttag
     7441 cattaataga aaacaaccat gaaacattaa atgacgatga tgtcgcaatg attttttcag
     7501 gcagcccgca tgccggtttt atgagtcagc gttatgccga aaaattctat tttcacgatg
     7561 aataccaaca acttgatttg taacgtaaaa atatagggaa actggatagc cagtataaac
     7621 gcgctttggc gtttacttta atgcgtcgcg ccatgatacg taaaatgccc tatacggaag
     7681 atatgcgccc gggcgatacc gctaatcctt atggcgcgtc caaagcgatg gtggaacgga
     7741 tgttaac
//
LOCUS       DAWKQJ010000090         7671 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000090 DAWKQJ010000000
VERSION     DAWKQJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.090"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            200..1735
                     /locus_tag="WJL20_001384"
     CDS             200..1735
                     /locus_tag="WJL20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7683535.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVTAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1732..1824
                     /locus_tag="WJL20_001385"
     CDS             1732..1824
                     /locus_tag="WJL20_001385"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ7683536.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1860..3104)
                     /gene="lysA"
                     /locus_tag="WJL20_001386"
     CDS             complement(1860..3104)
                     /gene="lysA"
                     /locus_tag="WJL20_001386"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ7683537.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEDVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3115..3285)
                     /locus_tag="WJL20_001387"
     CDS             complement(3115..3285)
                     /locus_tag="WJL20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7683538.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3356..3679
                     /gene="cyaY"
                     /locus_tag="WJL20_001388"
     CDS             3356..3679
                     /gene="cyaY"
                     /locus_tag="WJL20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ7683539.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3710..4129
                     /locus_tag="WJL20_001389"
     CDS             3710..4129
                     /locus_tag="WJL20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ7683540.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4151..4657
                     /gene="luxS"
                     /locus_tag="WJL20_001390"
     CDS             4151..4657
                     /gene="luxS"
                     /locus_tag="WJL20_001390"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ7683541.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4803..7598
                     /gene="polA"
                     /locus_tag="WJL20_001391"
     CDS             4803..7598
                     /gene="polA"
                     /locus_tag="WJL20_001391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ7683542.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         1760 a         2073 c         2086 g         1752 t
ORIGIN      
        1 cagcccctaa aatttttcgt tttcaaacct tcaccgcttg ccatcagcgt taaatttttt
       61 tacgataagc acatagattg taaacaatcg gtcacaagcc ggtttgtttt ttcagaagac
      121 attatccctg tcagacgctg tttctatata ttttgcctat aacggcttgt ttttaataaa
      181 taattcaaga ggtatcaacg tgtctgattc caagacgaaa gaacgcgcca cattcggcac
      241 gcgccgcgcg tttatgattg ccgccatcgg gtccgccgtc ggcttgggca atatttggcg
      301 tttcccctat attgcttttg aaaacggcgg cggcgcgttc atcctgccct atctggtcgc
      361 gcttctgacg gcgggcatcc cgctgctgct gctcgattat gccatcggcc accgttaccg
      421 tggttctgcg cccttggctt tccgccgcct cggacgatgg tttgagccgg tcggctggtg
      481 gaacgtgatg accaatatcg tcatctgcat ctattacgcg gtaattatcg gttgggcggc
      541 aagctatacc tattattcgg tcaacgccgc ctggggtgcg gatccgcagg gttttttctt
      601 taaggacttc ctgcaaatgg cgggcccgga agccttgggt ttggattttg tcggcaaagt
      661 cgccggtcct ttggcgggcg tgtgggtttt taccgccgcc attatggcgt tgggcgtgca
      721 aaagggcgtg gcgcgcgcct cgtcgttctt tatgccgctg cttttggtga tgtttttgat
      781 tatggtcggc atttcactaa ccctgccggg tgcggcaaag ggcttggacg cattgtttac
      841 gcccgactgg tcgaaactcg ccgattccaa ggtctgggtg gcggcatacg ggcagatttt
      901 cttttcgctt tccatctgct tcggcattat ggttacctat tcttcttatt tgaagaaaaa
      961 aaccgacttg ggcggaacgg ggctggtggt cggttttgcc aacagcagct ttgaactgct
     1021 cgcgggcatc ggcgtgtttg ccgcattggg ctttatggcg caggcgggcg gtaaggcggt
     1081 caacgaggtt gcctcaggcg gcatcggttt ggcgtttatc gcctttccga ccattatcaa
     1141 ccaggcaccg atgggctggc tgatcggcat attgtttttc ggttcgctgg tattcgccgg
     1201 cgttacgtcg atgatttcca tccttgaagt gattgtggcg gcgattcagg acaagctgaa
     1261 catcgggcgc gtcaacgcca cgctgctggt ctgcattccg atgggcattg tttccacgct
     1321 gctgttcggt acggcgacgg ggctgccggt tttggacgtg atggacaaat tcgtcaacac
     1381 ctacggcatt gttgccgccg gctttgttta tgttaccgcc atcatcatca gcggcaggct
     1441 gccggaatta cgcaagcacc tgaacgcttt gtcctccatc cgcatcggcg gcttgtggac
     1501 ggtctgcgtc gtggttaccg tcgtgatgct cggctatatg ctgtttaaag ataccagcgg
     1561 cctgatggag aaaaattacg aaggttatcc ggatggtttc ctcagtattt tcggctgggg
     1621 gatgtcggcg gcgttggtcg tgttcgggct gctgctgtcg ttgctgcctt ggaaacacgg
     1681 tcaggatttc aacgtcaaag acgaacacga acatgaacaa ggagaagaaa aatgagtact
     1741 tccgccattg tgatgatgat tgtctcaatc gtgataatct ggggagggct gctgctttcc
     1801 ctgttaaggc tgccgaacga gtaagccttt agagcgttaa aaatgccgtc tgaaccgctt
     1861 cagacggcat tttgatgttc ggcttgcagg caggcgagtt cgtttgccat ttgctgttct
     1921 aaggtttcgc gtcggcggat gagtttgtat ccgccaccgt taaccaacac ttccgccgca
     1981 cggttgcgcg cgttgtaatt gctcgccata ctggctccgt atgcgcccgc gctgcggata
     2041 agcagcaaat ccccttcttc gcaggcgatg gtgcggtctt tgccgaggaa gtcgccggtt
     2101 tcgcaaatcg gaccgacgat gttggcggtc agcgtcgcga tgtctttggt ttcgaccgcc
     2161 tcgatgtgat gataggcatc ataaagcgcc gggcgcatca aatcgttcat cgccgcatcg
     2221 accatcacaa agtttttctc ttcgccgtat ttgacaaact cgacgcgtgt cagcagcgaa
     2281 cctgcgttgc cgaccaggct gcggccgggc tcaagaatga gtttcagacg gcgtgtgccg
     2341 atcagttttt gaacggcttg ggcatacgcg cccaaatcag gcacatcttc gtcttggtaa
     2401 acaatgccga cgccgccgcc caagtccaag tgttccaaca caatgccttc ggcggcaagc
     2461 gcgtcaacca aaatcaaaat gcgctcgcag gcttcgacca gcgggcttaa gtcggttagt
     2521 tgggagccga tgtggcagtc gatgccgatg attttcaaat tgggctgttg tgcggcgtgg
     2581 cggtaggctt cgagcgcgtc ggcgtaggcg atgccgaatt tgttggcttt cagacctgtg
     2641 gagatgtagg gatgggtttt cgcatcgaca tcgggattga cgcgcaggga gacgggcgcg
     2701 gttttaccca aacgcgcggc aactttctga atacggtcga tttcggggat gctttccata
     2761 ttgaagcatt tcacgcctgc attcagcgcg aactcgattt ccgcctcgct tttgcctacg
     2821 cctgaaaata tggtttttgc cgcgtcgccg cctgccgcca aaacgcgtgc caattcgccg
     2881 ccggacacaa tgtcaaaacc gctccccaaa gaagcaaagt gtttgatgat gctcaggttg
     2941 ccattggctt taaccgcata gcagacgagc ggggagagct cggcaaaggc agtttggtag
     3001 tgttcaaatg cttcggtcag cgcggattgg ctgtacacat aaagcggtgt gccgaatgct
     3061 tcggcaaggc gggggtaggg gacttgttcg caaaataggg tcatattttc gttttcattt
     3121 ttgggttgga ggggcggatt gcggtttgcc ttgaagctgc aaaccggttt ggattacgcc
     3181 gaaacgcgcc ttgtcgcctt ctttgggcag gtagaggtcg cctttgtaac cgcaggccga
     3241 gagcaggagg gcggttgccg ccgcaaaaaa tacgccgtat ttcatcggta aacttccttc
     3301 ataagcgcga atgtggcaag attcggcatc ttaaacaaaa aacacgcaaa aagctatgat
     3361 gaccgaaagc gagtttatcc gcgcgagcga agcattattt gaacacatcg aagaccaaat
     3421 cgacgaaaac ggctgggatt tcgactgccg gtttgccgga aacgtcctga ccatcgaagc
     3481 cggagacggc acgcaaatca tcgtcaaccg ccacacgccc aatcaggaat tgtggattgc
     3541 cgcaaaaagc ggcggctacc atttcgccga acaaaacggc aaatggctgg caacgcgcga
     3601 cagccgcgat ttttatgatg ttttgaacga agcactgagc gcggcttcgg gcgaagcggt
     3661 tgagattgag gaattgtgat tcaatttgat ataaaaagat tgttacccaa tggcacaatt
     3721 acctctatat cggactgccg aaatcggaaa ttttactgtc ggcacgccta aagttttaga
     3781 atcattttcc aaacatatcc cttatggtgt cgtctttgaa gacgacggcg acacgggcta
     3841 cttctatgct gcttcgcaag acgggatttt agatgccttg cacatctaca atgtcgaaga
     3901 tgtatccgac aaacatatcc ccaatcatgt cttgatttta tgggatgatg cctgcaccat
     3961 agccgcattg tgtgtcaacg actacattca tgccgtctat gattttgtcg aacaggcagg
     4021 atattgccgc aacggcttcc ctgaagcagg cggcgaatgg gtgaaagtcg aaaaccgcgt
     4081 cttggatgat gaattgctgg acaaaatcct atcccgagaa cctacataac cctcacaaaa
     4141 ggatacccaa atgcccctac tagacagttt caaagtcgat cacacccgta tgcatgcccc
     4201 cgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc gacaccatta ccgtgttcga
     4261 cctgcgcttt tgcattccca acaaagaaat cctgcctgaa aaaggcatac acacgctgga
     4321 gcatttgttc gcaggtttta tgcgcgacca cttgaacggc aacggcgtgg aaatcatcga
     4381 catttccccg atgggctgcc gcaccggttt ctacatgagc cttatcggca cgccttccga
     4441 acagcaggtc gccgatgcat ggctcgcctc gatgcaggat gtgggcaatg tcaaagacca
     4501 aagcaaaatc cccgagttga acgaatacca atgcggcact tatcaaatgc actcgctcgc
     4561 cgaagcgcag caaatcgcgc aaaacgtgtt ggcgcgcaaa gtggcggtga acaaaaacga
     4621 agagctgacg ctggatgaag ggctgctgaa cgcctaatcc gccaaaaatg ccgtctgaac
     4681 aagggtttca gacggcattt gccttttccg ttataatccg gggttgtgcg gggtcgggtt
     4741 ttaagccggc atcgtccttc cctatttttt tctgtccctt atcggtttta agcgggtttt
     4801 ttatgtccaa cagacctaca ctcctcctcg ttgacggctc gtcctacctc taccgtgcgt
     4861 atcacgcgat ggggcaaaac ctgaccgccc ccgacggcgc gccgacgggt gcgctgtatg
     4921 gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc gcacgattat tgcgcggtgg
     4981 tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt tgaagaatac aaggcgacgc
     5041 gcccgccgat gcccgacgat ttgcgcccgc aggcggaagc actgccggat ttagtgcgcc
     5101 tgacaggctg gccggtattg gtgattgggc aggtggaggc ggacgatgtg atcggcacgc
     5161 tggcgaaaca gggggcggaa catggtttgc gagtcattgt ttcgaccggc gataaggaca
     5221 tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac gatgagcagc gaaacgctgg
     5281 acattgaagg cgtgaaggca aaattcggcg tgcgccccga ccaaatccgc gattatctcg
     5341 cgctgatggg cgacaaggtg gacaacgtgc cgggcgtgga aaaatgcggc ccgaaaacgg
     5401 cggtgaagtg gctggaagcc tacggctcgt tgcaaggcgt gatagaacac gcttcggaaa
     5461 tcaagggcaa agtgggcgaa aacctgcaag ccgcgctgcc ccaactgccg ctgtcgtatg
     5521 atttggtcac gattaaaacc gatgtggact tgcacgccga gctttcagac ggcatcgaaa
     5581 gcctgcgccg tactacgccg aaatgggcgc agctggttgt cgatttcaaa cgctggggct
     5641 tccgcacctg gctgaaagaa gcggaatcaa acatgaatac cggctcgacc gatgatttgt
     5701 tcggcagcga cagcatcggc gagcaggcgg ctttgaatgc ggaaatgccg tttgaaaaac
     5761 aagccgaaaa agccaccgcc cccgaaaaac tggattatca agccgttacc accgaagcgc
     5821 agtttgccgc tttgttggac aaactgtcgc gggcggacac aatcggcatc gatacggaaa
     5881 ccacgtcatt agacgcgatg aacgcctcgc tggtcggcat cagcatcgct ttccaagcag
     5941 gcgaagcggt ttacatcccc gtaggacaca gcctgaccgc cgcgcccgaa cagcttgatt
     6001 tgcaagacgt attaggccgt ctgaaaccgc atttggaaaa ccccgccctg aagaaaatcg
     6061 ggcaaaacct caaatacgac caacacgttt tcgctaacta cggcatcgcc ctgaacggca
     6121 ttgccggcga cgctatgctc gcttcctaca tcatcgaaag ccatcttgga cacggcttgg
     6181 acgaattgtc cgaacgttgg ctgggcttgg aaaccattac ctacgaatca ttgtgcggta
     6241 aaggcgcgaa gcaaatcagt tttgccgatg tcgccatcgg gcaggcgacc gaatacgccg
     6301 cccaagacgc cgatttcgcc ttgcgcctcg aagcgcacct gcgcgcgcaa atggacgcca
     6361 aacagcttga aatgtatgaa aaaatggagc tgcccgtcgc gcaagtattg tttgaaatgg
     6421 aacgcaacgg tgtgcaaatc gaccgcgccg aactcgcccg ccaaagcgcg gaactcggcg
     6481 ccgagctgat gaagctcgaa caagaagcct acgccgccgc agggcagccg ttcaacctca
     6541 actcgcccaa acagctgcaa gaaatcctgt tcgacaaaat gggcatcccg acaaaagggc
     6601 tgaaaaaaac cgccaagggc ggcatttcca ccaacgaggc cgtgctcgaa cagctcgcgc
     6661 ccgactaccc cttgcccaaa atcatcctgc aaaaccgcag cctggcgaaa ctcaaatcca
     6721 cctacaccga caaactgccc gacatgattt ctccccggga taaccgcgtg cataccacct
     6781 acgcccaagc cgtcgccatt acaggacgct tggcaagcaa caaccccaac ctgcaaaaca
     6841 tccccatccg caccgccgaa ggccgccgtg tgcgccgcgc ctttaccgcg ccaccgggca
     6901 gcgtcatcgt ttccgccgac tattcccaaa tcgaactgcg catcatggcg cacctctctg
     6961 gagacaaaac cctcattgcc gcattccaaa acggcgaaga cgtacaccgc cgcaccgccg
     7021 ccgaagtgtt cggcactgcg cccgaaaacg tctcgcctga gcaacgccgc tacgccaaaa
     7081 ccatcaactt cggcttaatc tacggtatgg ggcaatacgg ccttgccaaa tcattgggca
     7141 tcgacaacct ttccgccaaa aactttatcg accgctactt cgcccgctac cccggcgtcg
     7201 ccgaatacat gcagcgcacc aaagaacaag ccgccgccca aggcttcgtc gaaaccctgt
     7261 ttggccgccg cctctacctg cccgacatcc gcaacaaaaa cgccaacgcc cgcgccggag
     7321 ccgaacgcgc cgccatcaac gcccccatgc aaggcaccgc ctccgacctt atcaaacgcg
     7381 ccatgataga cgtgtcccgc tggctggttt cagacgacct gaagagcaaa ctgattatgc
     7441 aggtgcatga cgaactggtg ctggaagtcg ttgaaaccga actggatttt gtcaaagaaa
     7501 aactgccgca gattatggcg aaagtgggcg gcggattatt ggatgtaccg ctggtggctg
     7561 aggttggcgt aggggagaat tgggaagagg cacattgatt gaaaggtgtt attatgctat
     7621 ctttatttaa ataaaattta atttttggta tattttttct aaatgttcct a
//
LOCUS       DAWKQJ010000091         7639 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000091 DAWKQJ010000000
VERSION     DAWKQJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.091"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(476..3079)
                     /gene="pepN"
                     /locus_tag="WJL20_001392"
     CDS             complement(476..3079)
                     /gene="pepN"
                     /locus_tag="WJL20_001392"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ7683543.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(3215..4009)
                     /locus_tag="WJL20_001393"
     CDS             complement(3215..4009)
                     /locus_tag="WJL20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7683544.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4759..5627)
                     /locus_tag="WJL20_001394"
                     /pseudo
     CDS             complement(4759..5627)
                     /locus_tag="WJL20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
     gene            complement(5678..6214)
                     /gene="ruvC"
                     /locus_tag="WJL20_001395"
     CDS             complement(5678..6214)
                     /gene="ruvC"
                     /locus_tag="WJL20_001395"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ7683545.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASRLNPSGMQVKRGRFQ"
     gene            complement(6217..6456)
                     /locus_tag="WJL20_001396"
     CDS             complement(6217..6456)
                     /locus_tag="WJL20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ7683546.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6486..7496)
                     /gene="dusB"
                     /locus_tag="WJL20_001397"
     CDS             complement(6486..7496)
                     /gene="dusB"
                     /locus_tag="WJL20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ7683547.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLIS
                     DPTLRNTKKTLHRSDFADEGGVVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRPN
                     AF"
BASE COUNT         1724 a         2006 c         1987 g         1922 t
ORIGIN      
        1 agtatattaa tgctggcaat aatttggtac agtctatgtc tgtgttcggt tctaatactg
       61 ctgcgacagg aggaaatgtg gatgccaata tacaatccgt acagcagccg ttattggtaa
      121 cgccatctgc ataaggagcc taattacatt catggcttaa actgaaaaac agcaatcaag
      181 tttattttga ttgctgtttt tcttaatatt gggataaggg tcgtatttta attaacctta
      241 atcggtgcac ttctagcaat atagtggatt cacaaaaacc agtacagcgt tgcctcgcct
      301 taccgtacta tctgtactgt ctgcggcttt gtcgccttgt cctgattttt gttaatccac
      361 tataattaat atgactttgc ggccgttttg ccattgcgta ataaaacgat ggggaagtga
      421 tgataaaacg tgtgtgtaac tatatcagac ggcattgttt ttctgtttga cggcctcaat
      481 ccaaaatttt gccgacgatt tcgcccacgt ctttcgacaa tccttcctgc gcccgaatgc
      541 gctgcaatgc ttgtttcacc aagtttttgc ggtgcggctc gagcttgttg cagaggttga
      601 acgcctgcac caaacgggcg gcgacctgcg ggttgaagcg gtcgatttcg atgactttgt
      661 cggcgatgaa gcggtagccg ctgccgtctt ctgcgtggaa atgcgggacg ttgcggctga
      721 agctgccgat gagcgaacgg gctttgttgg ggttttcgag gctgaatttc ggatgctgca
      781 aggcggtttg aacctgttgc agggtgtcgc tgcggcggct tgagccgata agggcgaaat
      841 atttgtccat caccagcgcg tcgtctgaaa acttgtcggc aaactgcgcc agcaggcggt
      901 tgcgcgtatc gctttcgttg ccgttgacgg cggacaggat gccccattcg tgggtcatgt
      961 tttgcgccat ttcgccgtat ttttcggcaa cggtttcgat gtgcgcgggg tcggcgcgca
     1021 ggacgaaggc gcggcagacg ttgcgcagcg tgcgccagcc ggcagcttcg gggctgtact
     1081 cgtagctttg gttttcctgc ttcgccgcct gacggttcaa ttcgtgccat ttcggcagga
     1141 agcggacggc aagcgtgtcc aacaaggctt cgcgcgcctg atggtagcgc agcgggtcga
     1201 tgttttccgc gccgtcccac agctcggctt cagacggcac gcccaaaagc agggctttga
     1261 aggcgttgtc taagaggtcg tctgaaatga ctttttcgac ggcggcaagc agtttttcgt
     1321 gtttcggcag ttcaataccg tctgaaagtg cggcaaggtt ggcggcgacg gcacggcggt
     1381 agagcgtttg tgcggcttcc cagcgcgtga aggcgtcgct gtcatgggcg agcagaagca
     1441 gcaggtcgtc gtcgctgtac ggatagttca gatgcaccgg cgcgctgaac ccgcgcagca
     1501 gcgagggaac gacggcttcg gttacgcctt cgagcaggaa gatctgttcg gcttcggtca
     1561 gcagcaatac ggcttcggtc gcgcgtttgc cctgataatc gaatgccgcc gcttcgccgt
     1621 tgcggttcag cagcccgatt ttgacgggaa tcatcatcgg ctgcttgtcc gccatatcgg
     1681 gcgtgggcgg cacggtttgt ttaatggtca actcgaaaat attgtttttc agacggcttt
     1741 ccgcttccaa aacgggcgtg cccgcctggc tgtaccacaa ggcgaactgg tcgagattga
     1801 tgccgttcgc gtccgccatc gccgcgcgga aatcgtcgca ggtcacggcc tgcccgtcgt
     1861 gtcgttggaa atagagcttc atgccttttt ggaagccctc ttcgccgagc aaggtgtgat
     1921 acatccgcac cacttccgcg cctttttcat aaacggtcat ggtgtagaaa ttgttcatct
     1981 cctcatagct ggcggggcgc accggatggg cggtcgggcc tgcgtcttcg gggaactggt
     2041 gctggcgcag caggcggatg ttctcgatgc ggcgcacggc gcggctggcg cggtcgccgg
     2101 aaaattcttg gtcgcggaac acggtcaacc cttctttcag cgaaagctgg aaccaatcgc
     2161 ggcaggtaac gcggttgcct gtccagttgt ggaaatactc gtgtccgacc acggattcga
     2221 tgccttcaaa atcggtatcg gtggcggtac ggctgtcggc aaggacgaac ttggtattaa
     2281 aaatgttcag ccccttgttt tccatcgcac ccatattgaa atcgcccacg gcgacgacca
     2341 tgaaaatatc caagtcgtat tccaaaccga agcgcgtttc gtcccacttc atcgcgtttt
     2401 tcaaggattc cacggcaaag ccgaccttag gcttgtccgc ctcggcagtg taaaactcga
     2461 ttttgacgtt tctgccgccc atcgtggtga aacagtcttc cgtgaccgcc aaatcgcccg
     2521 cgaccaaagc aaacagatag ctcggtttgg caaacgggtc ttcccatttc acccaatggc
     2581 ggccgtctga acactcgccg ccgtcgattt tgttgccgtt ggagagcaaa acgggatagc
     2641 gttttttgtc cgcaacgatg gtggtcgtga atttggacat cacatccgga cggtcgatgt
     2701 agaacgtgat tttgcggaag ccctccggct cgcactgggt aaacaggtta ccggcggacg
     2761 catacagccc catcagcgat ttgttttccg ccggcaggat ttcggtttcc acttcgacgg
     2821 tgaagcgttc ggacggcacg tctgcaatcg tcagcgtctc accttccaac acataatccg
     2881 ccgccgcccc gttgattttg acggacaaga gtttcgccga accgtccaac accagcggct
     2941 cccctaccct ctgcggctca accgtcaaac gcgacttcac gacggtttgc ggttcggcaa
     3001 tatcaaaatg caggtcggtt tcaaggatgc ggtaggcagg cgtttggtaa tctttcagat
     3061 aacggacgat tttgctcatt tttttctttc aatattgttt ggtttgactg gaaaaggctt
     3121 cagacggcat aggcaaaccc tgcaccgata tgccgtatga aaccgaaaca tcagtgcgga
     3181 cgcaccgtca aacaaccggt tttaatttca atctttattt ccacgcttgg acaaattctg
     3241 cccaatgcgg tttgtcttct gtttgggcgg aaagatagtt tctgatccgc ttgatttcca
     3301 tttcgtattc gtccgcgtcg aatctgccgc tgaccagaca gaaacgcaga tacatcaaat
     3361 acgtattcac ggcatcggtt tcacaataat tgcggatttc cttcagcctg cccgcgtgga
     3421 acgcatccca caccttgctg ccgtccatac ccagcttgcc tggaaaaccg cacagtttcg
     3481 ccatatcgtc cagcggcacg tttgccctcg gctggtaaag cgcgagcaaa tccatcaaat
     3541 cgcaatgacg ttggtggtag cggctgatgt agttgttcca cttgaaatcg cggctgtcgc
     3601 cgaaatcgcc gtcacccata tcccaatagc gcgcggcgtt gatgccgtgt agcagcgaac
     3661 ggtaatgcag aaccggcagg tcgaaaccgc cgccgttcca actgaccagt tgcggcgtat
     3721 gtttttcaat caactcgaaa aatttggcaa tcacgacttc ttcgccatcg tccatctcgc
     3781 cgattgtacc gacatggact ttatcctgcc cccaacgcat gcagcacgaa atcgccacaa
     3841 tctgatgaag atgatgctgc ataaaatccc cacccgtctg agcacggcgt ttttgctggg
     3901 caaacagcac cacttcatcg tcgggcagcg aggacggcag ctcgtacaat gttcggatac
     3961 cctgcacatc gggtacggtt tcaatatcga aagccaaaat cgtggtcatg acagcacctt
     4021 gtatttaaaa cagacttgcg cctattgtgt cattaaaagg cggataaaaa aagaggcaac
     4081 cccccacagg attgccccaa tacctcaaat cagagattta cgcttcacaa acaatacagg
     4141 ctttcgcctg cggctttgcc cgcgtagctc aactctacgc cggcaaactt ttgtttcacc
     4201 gtttccgatg aaaccccgac caatcgcaag actgaccgga aaatcctttc agacggcatt
     4261 tcctgcctgt cgtgtaattc catgtagcga aatgtacgcc attttctacg ctttgccaag
     4321 cattttttac aatataaatg tcaaaacatt aattttataa aattgctgaa aatattaaat
     4381 atatggattt ttatttttta tatttcaata aatataaatt taattttgat ttatatttaa
     4441 atttaagcat aaaatgtcaa atattaaagg aaacatgaaa ggcatatatt aaatatttat
     4501 ttataacgct atgtttttaa agaaaattaa ttttaatata ttaactagat tgtctgcata
     4561 tattcatagg tttgcggtat ttcttccaaa acctgcttcg aatttcccga ccaagtctta
     4621 aaaatattgt tttttgagat acttaaatag cagcgattat caaatgaaat ctgttcatat
     4681 aatctgccat tttgcattta aaaaaacaat caggagtttc gactcgaaac gcctgatatg
     4741 ttttgtaatt ttacgtagtc agtaaaaatc ggggctgcct tccggacggg ttttaaaacg
     4801 cttgtgcagc caaaaatatt gttccggatg ttcgcgcacc ctgtcttcga taaaacggtt
     4861 catgcgctgc gcgtcggctt tcgcgtcttc acccggaaag gattcccaag cagggtagaa
     4921 atgcaatgta accgtattgt cctcctccct gacgggaatg gcgggtatca ctttggcatt
     4981 tgcaagcgcg gcaatgcggc tcaatccggt aatcgttgcc gtctgaatac cgaaaaaatc
     5041 cacaaaaacc gaatcgttgc gtccgaaatc ctgatcgggc agatacagaa acggcgcgct
     5101 gcctttgcgg aactgtttga cgagggcgcg cagcccttcg gtgcgcccga taaggaaaac
     5161 gttgtgataa cggttgcggc ctttcaaaat ctgttcgtcc aatatcttgt ttttttggtg
     5221 ggaatacata ctaattagcg gaacatcctg attaagcgcg tacaccgcca tctcgaacgc
     5281 ggtaaagtgc ggatacagga tgatgacttt tcccccgccg ccagcgcgtc gtccaaataa
     5341 tgcttattgc ggtagcgcac cagcgatttc aaacgcccgg cgggcgcgta ccaatataag
     5401 ccgtattcga gcatcagttt cgccatatgt ttgaaatgct gtttcaacac ggttttacgc
     5461 ttttcctcac tccattcggg aaaacatttc gccaaattga tttcgccgat acggcggcgc
     5521 ggtttgacca gaaggtaggc aagcaaaccc gtcaggtcgg caatcttgtg cagcagcgca
     5581 aacggcagaa actgcaaaac atacagtaca aaaaatataa atttcatctc gatacacatt
     5641 ttcttttcag acggcaaaat acaaatgccg tctgaaacta ttgaaacctt ccgcgcttga
     5701 cctgcatccc cgacgggttg agccgggacg caagcccatg gttgcgtaag gcgtgggtca
     5761 gcgcgacggc aagaccgtcc gccgcatccg cctgcggcgt tcccgaaagc cccagcatct
     5821 gcaccaccat atgctgcacc tgttcttttg ccgccttgcc tttgccgact accgcctgtt
     5881 tgacctgcaa ggccgtgtat tccgaaacgg gcagcttatg gctgaccaat gccgccaatg
     5941 ccgcgccccg cgcctgaccg agcatcagcg tcgatgcagg attgacgttg acgaacacct
     6001 gttccaccgc cgcctgttga ggcttgtaaa cggtaacgac ttcgccgatg tgccggacga
     6061 tgacggcaat cctgtctgcc agaggcgcat cggcaggcgt tttgatgcag cctgaggcaa
     6121 cgtaaaaatg atcgcgcccc ctgacatcga tgatgccgaa acccgttacg cggctgcccg
     6181 ggtcaatacc caagatacgg acggttgccg acatattcac agcaaaccgt gttgaatcag
     6241 cttcttacgc agggtattgc ggttcagtcc caacatgacg gaggctttgg actgattacc
     6301 gccgcattgc tccatcacgc acaccagcag cggcttttcc acctgatgca ataccatatc
     6361 gtacacgccg caaggttcgg taccgttcag gtctttgaaa tattgttcca aattttgtct
     6421 gatacattgg gaaatatcgg gaagggtatg gggcatgatt gcaccttcaa aggataatca
     6481 agtgttcaga aggcatttgg gcggtaggcg cacgcccaac ggtcggtttt tccggcaagt
     6541 ctttcaagat aacctgcaag catgtcgtat tgcgccgccg cactgtccaa gcggttgatt
     6601 tcacgacgtg tctgttcgcc gtcgggcatt tcgtcgatgt accagcctat gtgtttgcgt
     6661 gcaatgcgca caccggcggt ctcaccataa aacgcgtgca tggcgcggac gtggttcaaa
     6721 atggcggcac tgcattctgc caaactcaag gcaggcggta aaacaccgtg ttcggcgtaa
     6781 tgtttcaaat cgcggaagaa ccacggtctg ccttgcgcgc cgcgccctat catgatgccg
     6841 tcggcggcgg tttgtttgag gacggcggcg gctttttgcg gcgaagtgat gtcgccgttg
     6901 acccagaccg ggatgttcag acggcatttg gtttcggcga tgagttcgta acgcgcctcg
     6961 cctttgtaca tttgcgtgcg cgtgcgtccg tggacggcaa gggcggcgat gccgcaatct
     7021 tcggcgattt tggcgatgac gggcaggttt tgatggtcgt cgtgccaacc caaacgggtt
     7081 ttgagggtaa cgggtacacc tgccgcacgg acgacggctt ccaaaatggc ggcaaccagc
     7141 ggctcgttct gcatcagcgc gctaccggct tggacattgc agactttttt agcgggacag
     7201 cccatgttga tgtcgataag ctgcgccccg aggctgacgt tgtaacgcgc ggcatcagcc
     7261 atctgctgcg gatcgctgcc ggcaatctgc acggcaacga caccgccttc atcggcaaaa
     7321 tcgctgcggt gcagggtttt tttggtattt ctgagcgtcg ggtcgctgat cagcatttcg
     7381 cacaccgccc aacccgcgcc gaaatcgcgg cagaggcggc ggaagggttt gtcggtaatg
     7441 cccgccatcg gtgcaagtgc gatggggttg tcgataaaat agccgccgat gtgcataatg
     7501 ggcccgcgtt tcaaaaaagt gcgacattgt acatttttta agcaggattt ccaatctccg
     7561 gacgcgcccg cgattggggc gggcaccgtt ttatggcata atccgcacac agatttcctg
     7621 ccccgccact cacaggcgg
//
LOCUS       DAWKQJ010000092         7566 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000092 DAWKQJ010000000
VERSION     DAWKQJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.092"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            24..419
                     /locus_tag="WJL20_001398"
     CDS             24..419
                     /locus_tag="WJL20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7683548.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL20_001399"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL20_001399"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ7683549.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1383..1730
                     /locus_tag="WJL20_001400"
     CDS             1383..1730
                     /locus_tag="WJL20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ7683550.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            1735..2328
                     /locus_tag="WJL20_001401"
     CDS             1735..2328
                     /locus_tag="WJL20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ7683551.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2392..3000
                     /locus_tag="WJL20_001402"
     CDS             2392..3000
                     /locus_tag="WJL20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ7683552.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3008..3664
                     /locus_tag="WJL20_001403"
     CDS             3008..3664
                     /locus_tag="WJL20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683553.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF
                     "
     gene            3704..4483
                     /gene="map"
                     /locus_tag="WJL20_001404"
     CDS             3704..4483
                     /gene="map"
                     /locus_tag="WJL20_001404"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ7683554.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4638..4937
                     /locus_tag="WJL20_001405"
     CDS             4638..4937
                     /locus_tag="WJL20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7683555.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5175..5549
                     /locus_tag="WJL20_001406"
     CDS             5175..5549
                     /locus_tag="WJL20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7683556.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5737..7203)
                     /locus_tag="WJL20_001407"
     CDS             complement(5737..7203)
                     /locus_tag="WJL20_001407"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ7683557.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AGQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1950 a         2284 c         1850 g         1482 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatgacg ataggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgttctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat ccttccacac cggccacgct caaagccgcc atcaccgcgc ttgcgcccac
      961 gacgggaacg actttaaacc cggcctcacg cacgcggccg gcgagtttgg cacccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacactga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctggaacata aaaaatgccg tctgaaaaac aaacattata aattataaag gttaaaccga
     1381 ttatgcgcct aaaccacaaa cagggcgagg caggggaaga tgccgcgctt gccttcctcc
     1441 aatcccaagg ctgcacactg cttgcccgca actggcactg cgcctacggc gaaatcgacc
     1501 tgattgtcaa aaacggcggt atgattctgt ttgttgaagt aaaataccgc aaaaatcggc
     1561 aattcggcgg tgtcgcatac agcatttccc catccaaatt attgaaactg caacgaagtg
     1621 tagagtatta tctgcaacag aacaggttga caaacgtacc gtgccgcctc gatgcggtac
     1681 tgattgaagg aaaccgcccg cccgagtgga tacagaatat tacaggttga cgatatgacg
     1741 acattacaag aacgcgttgc cgcccatttt gccgaaagca tccgtgccaa acaggaagcc
     1801 gaaaaagtac tggtcgagcc gaccgtacag gctgccgagc tgatgctgca atgcctgatg
     1861 aacgacggca agattctggc gtgcggcaac ggcggttcgg ccgccgatgc ccagcacttc
     1921 gccgccgaaa tgaccggacg ctttgaaaaa gagcgcatgg aactcgccgc cgtcgccctg
     1981 accaccgaca catccgcgct gaccgccatc ggtaacgact acggcttcaa ccacgtcttc
     2041 agcaaacagg tacgcgccct cggacgtgca ggcgacgtct tggtcggcat ttccacttcc
     2101 ggcaattccg ccaacgtcat cgaagccgtc aaagccgcac acgaacgcga tatgcacgtc
     2161 atcgccttga ccggccgcga cggcggcaaa atcgccgcca tactcaaaga caccgacgtt
     2221 ttgctcaacg ttccccatcc gcgcaccgcc cgtattcaag aaaaccacat cctgctgata
     2281 cacgccatgt gcgactgtat cgactccgta ctgctggaag gaatgtaacc cttttcagac
     2341 ggcatggcgc aaagcaatgc cgtctgaaac gcccaagaaa ggaagcaccc gatgaaaccc
     2401 aaaccgcaca ccgtccgcac cctgattgcc gccattttca gccttgccct tagcggctgc
     2461 gtcagcgcag taatcggaag cgccgccgtc ggcgcgaaat ccgccgtcga ccgccgaacc
     2521 accggcgcgc aaaccgacga caacgttatg gcgttgcgta tcgaaaccac cgcccgctcc
     2581 tatctgcgcc aaaacaacca aaccaaaggc tacacgcccc aaatctccgt tgtcggctac
     2641 aaccgccacc tgctgctgct cggacaagtc gccaccgaag gcgagaaaca gttcgtcggt
     2701 cagattgcac gttccgaaca ggccgccgaa ggcgtataca actacattac cgtcgcctcc
     2761 ctgccgcgca ctgccggcga catcgccggc gacacttgga acacatccaa agtccgcgcc
     2821 acgctgttgg gcatcagccc cgccacacag gcgcgcgtca aaatcgttac ctacggcaac
     2881 gtaacctacg ttatgggcat cctcaccccc gaagaacagg cgcagattac ccaaaaagtc
     2941 agcaccaccg tcggcgtaca aaaagtcatc accctctacc aaaactacgt ccaacgctga
     3001 ctcggcaatg ccgtctgaac cgccttcaga cggcattgcc cgacacccca aaagcacaat
     3061 caaaatggca aaaaaaccga acaaaccctt caggctgacc cccaaactcc tgatacgcgt
     3121 cgtattgctc atctgtatca ccgccatcgg cgcattggca ataggcatcg tcagcacatt
     3181 caacccgaac ggcggcaaaa ccctccaaac cgaaccgcaa cacaccgaca gcccccgcga
     3241 aaccgaattc tggctgccaa acggcgtagt cggacaagat gccgcccaac ccgaacacca
     3301 ccacgccgcc tcatccgaac ccgcacagcc ggacggcaca gacgaaagcg gcagcggact
     3361 gccgtcccct gccgcaccca agaaaaaccg ggtcaaaccg caacctgccg acacagctca
     3421 aaccgacagg cagccggacg acgccggaac acaagctgaa aacacactca aagaaacccc
     3481 cgtactgccc acaaacgtcc cccgtcccga accccgaaaa gaaacacccg aaaaacaggc
     3541 gcagcccaaa gaaacgccca aagaaacgcc caaagaaacg cccaaagaaa accataccaa
     3601 accggacacc ccgaaaaaca cgccgcccaa accccataaa gaaattctcg acaacctctt
     3661 ctgacccggc acggcaggca cacccgcaat ccaaggaagc attatgaacg gcatcatcat
     3721 caaaaccccc gaagaaatcg aaaaaatgcg cgagctgggc aaactcgtcg ccgaagccct
     3781 cgactacatc ggacaattcg tcaaacccgg cgtaaccacc gacgaaatcg acaaactcgt
     3841 ttacgactac cacgtcaacg tccaaggcgg ctatcccgcc cccctgcact acggcaaccc
     3901 gccctacccc aaatcctgct gcacctccgt caaccacgtc atctgccacg gcattcccga
     3961 cgacaagccg ctcaaagaag gcgacattat caacatcgac ctcaccatca aaaaagacgg
     4021 cttccacggc gactccagcc gtatgtttac cgtcggcaaa gtctccccca tcgcccaacg
     4081 cctgatcgac gtaacccacg cctccatgat ggcgggcata gaagccgtca aacccggcgc
     4141 gacactgggc gacgtaggtt acgcctgcca acaggttgcc gaaaacgccg gctattccgt
     4201 cgtacaggaa ttctgcggac acggcatcgg gcgcggtttc cacgaagccc cgcaagtgtt
     4261 gcactacgga aaaaaaggac agggctccgt tctaaaaccg ggtatgattt ttaccgtcga
     4321 accgatgatc aaccaaggca aacgccacct gcgtatcctc aacgacggct ggacggtggt
     4381 taccaaagac cgctccctct ccgcccaatg ggaacacgaa gtcttggtga ccgaaaccgg
     4441 ctacgaaatc ctcaccgtca gccccgcctc cggcaaaccc tgaagccgga cgtatccgcc
     4501 ccataaaaac aaacaatgcc gtctgaaaga aacggcagat atgatatata atataaaaac
     4561 aggcttgacc cggcacatta cgaaaacaaa gcaaatcgga atttgccccg caaccagaca
     4621 aacttaaagg aagttttatg aaaatatttg aaaatataga agatgttaaa gccatccgta
     4681 aaaagaccgg gctgaaccag atagacttct ggggcaaggt cggcgttacc cagtctggag
     4741 gctcgcgcta cgaaaccggc cgcaaaatgc ccaaacccgt acgcgaactg ctccgcctcg
     4801 tccatatcga atgcatcgat ttggcgaaag tcaacaaaaa agatatggaa atcgccgccc
     4861 tgttgaaaaa acaccatccc gacctgtatg ccgagttgtc caaacagacc aagtccgaaa
     4921 gaaaaaaaca aagttaaacc gcaacctccg gatgcccgac agtttttcat ttccgaaaaa
     4981 cgcaaacaat gccgtctgaa acaccggaca ggccgccgta tcccgcctgc cgcccctgcc
     5041 tcaaaccgcc gaaccgcccg aacccgcctt tttacaaact ttatccaatt tcctgtttat
     5101 ttcaggatac gccgacatta gaatgtcaaa cagctcgaaa cgggcaaact ccacatccat
     5161 ccaaaggaat aaaaatgaaa cttctgacca ccgcaatcct gtcttccgca atcgcgctca
     5221 gcagtatggc tgctgctgcc ggcacgaaca accccaccgt tgccaaaaaa accgtcagct
     5281 acgtctgcca gcaaggtaaa aaagtcaaag taacctacgg cttcaacaaa cagggcctga
     5341 ccacatacgc ttccgccgtc atcaacggca aacgcgtgca aatgcctgtc aatttggaca
     5401 aatccgacaa tgtggaaaca ttctacggca aagaaggcgg ttatgttttg ggtaccggcg
     5461 tgatggatgg caaatcctac cgcaaacagc ccattatgat taccgcacct gacaaccaaa
     5521 tcgtcttcaa agactgttcc ccacgttaat caggcaacaa aaaacagcgt tttcagaaat
     5581 gaaaacgctg tttttttgac cgttccatta ttcacaaaag ggaaaaaacg attacctgcc
     5641 ccgtgtatca aaacctgccc tgccggatga agggcataac cggcagggac ggcgtcaaca
     5701 ccatatgggg gtacggcttt tcttgaaaga ttcggcttaa atatccaata ctttcgcggt
     5761 ataggcgata atttcatccg ccctttcagg gttttcgttc aacttgatgc cgtaacccgg
     5821 taccagctct ttcagacggt cttcccaaga cggggcgcgc tcggggaagc attggtgcat
     5881 cagccggatc atcagcggca cagcggtcga tgcgcccggc gacgcgccca gcaatgcggc
     5941 gagtgagccg tcggcgtggg cgacaatctc cgtaccaaac tggagcacgc cgcctttttc
     6001 ggagtctttt ttaatgattt ggacgcgttg ccctgcggtg atgagttccc agtcgtcggg
     6061 gtttgcctcg gggtagtatt ccagcaggga ggcgaagcgt tcttctttgg ttttacgcaa
     6121 ttcgcccagc aggtatttgg tcagcggcat attcgcccag ccggcgcaca gcataggata
     6181 gaggttgtcc atatggatgg acagcggcaa atccataagc gagccttgct tgaggaagtt
     6241 ggaacggaag cctgcgtaag ggccgaacat aaggtggcgt ttgccgtcca cgttgcgtgt
     6301 gtcgaggtgc gggacggaca tcggcggcgc gccgacggaa gcctgcccgt acactttggc
     6361 gttgtgttgt ccggcggttt cggggttgct gttgcggaag aacaggccgg acacggggaa
     6421 gccgccgtag cccttgcctt cggggatgcc ggatttttgc agcagggtca gcgcgccgcc
     6481 gcccgcgccg aggaagagga agcgggtacg gagggtgagc tgcccgtcgg ggttgcgggt
     6541 atcggcggtt ttgagcaccc acgcgccgtc ggattcgcgt ttgatgtctt cgacgtggcg
     6601 gttgaactcg gtttttacgc ccttgccctg caaatatttc accatttggc gcgtcagccg
     6661 tccgaaatcg acatccgtgc cttcggcgga gtagttggcg gcgacgggtt ggttttcgtc
     6721 ccggccgcgc atcatcagcg gagcccaatc ggaaattttg ttccgatcgg tggaaaattc
     6781 catattttca aaaagttttt gggttttaaa cgcgtcataa cgtttttgaa gataagaaca
     6841 atggtcttca ttcatcacca aagacatatg cggcacggca ttgatgaagg aattgtcttc
     6901 caacttgcct tccgcgacca gcgtcgccca aaactggcgg ctgacatgaa actgttcggc
     6961 aatattgagg gcgcgcgccg gatcgataat cccatttgca cccaacggcg catagttcaa
     7021 ttcgcacagc gcggaatgcc ccgtgccggc gttgttccac gcgtttgacg attccaacgc
     7081 cacatcttcc aagcgttcaa tcagggtgat ttcccaagac ggttcgagtt ctttgagcaa
     7141 aacgcccaaa gtcgcgctca taatgccgcc gcccaccaag acaacgtctg tcgcttcagc
     7201 catggtttac tcctaaaaaa caggcatctt ctgcccttat ggttatttgc cgtactacaa
     7261 acgcctgaat cgcaaaagca gggaaaaccg gcaatggtgt gtgtccgagt atgctgtttc
     7321 ggggttggaa tgcgttgcaa gcatggcttc cgacaccgct tcaggggctt gtaatatgtt
     7381 atcgtgaatg tagtggattt tactgggaaa tgcaaagttt ttctgtcgcc cgccaagtcg
     7441 ggaaactgcg aaatgaaaaa taaaaatagt tatttatcta tatatatcaa atttttaata
     7501 gataaaaaat caaaattgtt tatatattaa tttttaaaag attgtcagca tattgcgtta
     7561 agtttt
//
LOCUS       DAWKQJ010000093         7506 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000093 DAWKQJ010000000
VERSION     DAWKQJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.093"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            205..1638
                     /gene="lpdA"
                     /locus_tag="WJL20_001408"
     CDS             205..1638
                     /gene="lpdA"
                     /locus_tag="WJL20_001408"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7683558.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNSDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1733..2020
                     /locus_tag="WJL20_001409"
     CDS             1733..2020
                     /locus_tag="WJL20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683559.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2119..3285
                     /gene="sucC"
                     /locus_tag="WJL20_001410"
     CDS             2119..3285
                     /gene="sucC"
                     /locus_tag="WJL20_001410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ7683560.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3296..4186
                     /gene="sucD"
                     /locus_tag="WJL20_001411"
     CDS             3296..4186
                     /gene="sucD"
                     /locus_tag="WJL20_001411"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ7683561.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4421..4750)
                     /locus_tag="WJL20_001412"
     CDS             complement(4421..4750)
                     /locus_tag="WJL20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7683562.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHAKNLSAGKQRRHERGIWQRRFYEHTVHDETDLQHCADYIHSNPIKQRMSRA
                     KTESCKK"
     gene            complement(4722..4865)
                     /locus_tag="WJL20_001413"
     CDS             complement(4722..4865)
                     /locus_tag="WJL20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683563.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIDFAGSLYGCAKT
                     ISL"
     gene            complement(5151..6353)
                     /gene="dcm"
                     /locus_tag="WJL20_001414"
     CDS             complement(5151..6353)
                     /gene="dcm"
                     /locus_tag="WJL20_001414"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7683564.1"
                     /translation="MLIPQTSLFDQENNRIISQRKLLEQSLLQGIPLNELIRQNQMSY
                     DDLIYIHHDKKPFEKRKNLVLSDFQKKENHIPIISFFSGAGGLDLGFEAAGFQHSMLI
                     EKIPLFCDTLRLNRPNWSVFNADVSETEEMVELIRNNIGSLSFDGVFIGGPPCQPFSI
                     ASNQRFSKTDKNFKRVGFEHKQNGNLLFNYVELIKIFRPKVFLIENVSGLMDIDNGKQ
                     LASVYCDLQQQGYSLYKPQIINSEYFHVPQQRIRLFIIGYLGDKKFSPLQPTNTPLAC
                     GNTFEIPIINQHNHITREHSAESILRYMELAYGQRDKKGRVDRLDPSKPSKTIIAGGT
                     KGGGRSHLHPYIPRTLSVRESARLQTFPDDYIFTGAVARQFTQVGNAVPPILAAQLAT
                     AIKHSYFE"
     gene            complement(6366..>7506)
                     /locus_tag="WJL20_001415"
     CDS             complement(6366..>7506)
                     /locus_tag="WJL20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7683565.1"
                     /translation="FDEIKKIAKGLYGINQCPKPRAKDIDIVRLSLTQETNLPFAPNS
                     SQYPVWRNYGRVFKCLLLATEHNGLICTTDLCADLVQNDEISCDDYLLHFATHFSYPS
                     PAFSGYQPSENRVFPVLAIIRALISKYKETGVNFLDMDEIIYKIASNNSITGIASFSF
                     SQLIMNKEAIKYDPRQIRELVKFISQFSFLSWYENKLFLDITDFSELHLLENQFIADT
                     QPQERLDSLEIMRLGKTNNNFLLMNEIVSLSIDDQAFSEGGKIKVTHLITERNSILRK
                     IYFSKTQNAQICRMCGLDTQRSYEWSEHIIEVHHLLPLSSPVRVEDKTTSLKDLVGIC
                     PTCHRATHKYYTFWLNLHKKKDFVSYEEAYHVYEEAKSKVKRIYK"
BASE COUNT         2156 a         1638 c         1692 g         2020 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac gaacgattga ggccgtctga
       61 aacatttgat tcgaatgaat cggcagatat ggactttcag acggcttttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat tggtgcaggc
      241 ccgggcggat acgttgccgc catccgtgcc gcacaactgg gtttcaaaac tgcctgtgtc
      301 gatgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg cttgaacgta
      361 ggctgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc agcgcaacac
      421 gagtttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgtggc caaaatgatt
      481 gagcgcaaag atgccatcgt aaccaaactg accggcggcg tgaaattcct gttccagaaa
      541 aacaaagtaa ccagcctgtt cggtacggct tcctttgccg gcaaaaatag cgatgcttac
      601 caaatcgaag tcgataacaa aggtgagaaa accgttatcg aagccaaaca cgtcatcgta
      661 gccaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgacaatgt gaacgtattg
      721 gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt gatcggttcc
      781 ggtgtgattg gtttggaaat gggttccgta tggaaccgcg tgggtgcgga agttaccatt
      841 cttgaagccg cgccgacctt cctggctgcc gccgaccaac aaatcgccaa agaagccttc
      901 aaatacttca ccaaagagca aggtctgagc atcgaattgg gcgtgaaaat cggcgacatc
      961 aagtctgaag gcaaaggtgt ttccgttgct tacgaaactg ctgccggcga agccaaaacc
     1021 gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa aggcctgaac
     1081 gctgaagccg tcggcttgga aaaagacgag cgcggcttta tcaaagtaga tggcgaatgc
     1141 cgcaccaacc tgcctaatgt atgggcaatc ggcgacgtgg ttcgcggccc gatgttggca
     1201 cacaaagcca gcgacgaagg cgttgccgtt gccgagcgca ttgccggtca aaaaccgcat
     1261 atcgatttca acaacgtacc gtttgttatt tacaccgatc ctgaaatcgc ttgggtgggt
     1321 aaaaccgaag aacagcttaa agccgaaggc gtggagtaca aaaaaggtac ttcaggtttt
     1381 ggtgcgaatg gtcgcgcatt ggcaatgggc aaagccaaag gtacggttaa agtgttggca
     1441 gacgccaaaa ccgaccgcat cttgggcgta cacatgattg gtccggttgt cagcgaattg
     1501 gttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat cgcccgcatt
     1561 atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc ggccgacaaa
     1621 cgcgctttgc acggttgata gacattaagg cctccgacaa attgaatgtt ccgagagctt
     1681 cgttttctga tttataattc cgtcagacaa acaaacagca tttacattca ttatgaacaa
     1741 agaaatagtc ggtattttct ttataccggc gggcatcatc agcatgtgta tggccgcatt
     1801 gtggcagatg tatgtgatga tgaccgaaac ttatacgctc aaccgtttca aagataaaga
     1861 attggtttgg cgcgtggcat tgttgtttat cagtttcagc cttgccgttt atctgctctg
     1921 tccgaattcg cgtaaaaaag gcatcgtctt ttttattctc gggggaggcg gtgcagccat
     1981 gtatctgctg gcgcggatgt ggttgccttt cagcaagtga aacgacgatt ttccgaccgc
     2041 cgaaaggtag tctgaaacgc acgggcttgc catttggagg caaactcggg gcattccact
     2101 aatctaaagg agaaatccat gaatttacac gagtatcagg ctaaagaact gctggctggc
     2161 tacggtttgc ccgtacaagg cggtattttg gcacacaacg gcgaagaagc cgctgcagct
     2221 tacgacaaat tgggcggcaa attcgctgtt gtcaaagcac aagtacacgc cggcggccgc
     2281 ggtaaagcgg gcggcgtaaa agtcgttaaa agccgcgaag aagctaaaga agtggctgaa
     2341 agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggcca acctgtcaac
     2401 agtgttttgg tttgcgaaga catgtatccg gttcaaaccg agctgtactt gggcgcagtg
     2461 gttgaccgtt ctacccgccg cgttacattc atggcttcta ccgaaggcgg cgtggaaatc
     2521 gaaaaagttg ctgccgaaac tccagaaaaa atcttcaaag taaccgttga tccgctggtc
     2581 ggcctgcaac cttgccaagc tcgcgaagtt gccttccaac tgggcttgaa agacaaacaa
     2641 atcaacgagt tcgtcaaact gatgaccggc gcgtacaaag cgtttgtcga aaacgacttc
     2701 gccctgtttg aagtcaaccc gctggcagtt cgcgaaaacg gcgcactggc ttgcgtggac
     2761 ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc caaaaatcgc tgaattgcgc
     2821 gacaaatctc aagaaaacga acgtgaactg aaagcttctg aattcgacct gaactatgtt
     2881 gccctggaag gcaacatcgg ctgtatggtt aacggtgccg gtttggcgat ggccaccatg
     2941 gacatcatca aactcaaagg cggtcaacct gccaacttct tggacgttgg cggcggcgca
     3001 accaaagacc gcgtggttga agcgttcaaa ctgattttgg aagacaaatc cgttaaaggc
     3061 gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga agccatcgtg
     3121 gcagccgtta aagaaatcaa cgtcaacgtt cctgtcgttg ttcgtttgga aggtaacaac
     3181 gccgaactcg gcgcgaaaat cctgaacgaa tcaggcctga aactgacttc tgcagacggc
     3241 ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga aaagaatgag
     3301 cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta aaaacggtac
     3361 tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg ttaccccggg
     3421 caaaggcggt caaacccacc tgaacctgcc cgtgttcaac accatgaaag aagctgttaa
     3481 agaaaccggc gcggatgcat ccgtaattta cgttcccgct ccgtttgtgt tggattctat
     3541 cgttgaagcc gttgattcag gcgtaggctt ggtcgttgtg attaccgaag gcgtacctac
     3601 tttggacatg ctcaaagcca aacgttattt ggaaaccaac ggcaacggta cccgtttggt
     3661 cggccctaac tgcccgggcg tgattactcc gggcgagtgc aaaatcggta ttatgccggg
     3721 ccacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat tgacttacga
     3781 agctgtggca caaaccacca aactgggctt gggtcaatca acctgtatcg gtatcggcgg
     3841 cgacccgatt cccggtatga accaaatcga cgcactgaaa ctcttccaag aagacccaga
     3901 tactgatgcc atcatcatga ttggtgaaat cggcggtact gcggaagaag aagcagccga
     3961 atacatccaa tccaacgtaa ccaaacctgt tgtaggttac attgctggtg ttaccgctcc
     4021 taaaggcaaa cgtatgggcc acgccggtgc gattatttcc ggcggtaaag gtactgccga
     4081 agaaaaattc gccgctttcg aaaaagccgg tatcgcttac acccgcagcc ctgccgagtt
     4141 gggcactacc atgctggaag tgttgaaagc aaaaggtttg gcataatcag gtttgacaac
     4201 tgattgaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc attttgtttg
     4261 tcatttcaaa aagaggcagc ctcaacatac ccacattatt tttgcccttt gaggcaggca
     4321 gaatatttat cggtcaaaca ggagcaaacc agtaagtcag gttgcacacg ccttgtttga
     4381 agttggggaa tgataactat cgcatttgaa aagactgata ttattttttg caggattctg
     4441 ttttagcacg cgacattctt tgtttgatag gattggaatg gatgtagtcc gcgcaatgct
     4501 gcaaatccgt ttcatcgtgc acggtgtgtt cgtaaaaacg ccgttgccaa ataccgcgtt
     4561 cgtgccgccg ctgtttgccg gcggataagt ttttagcatg aggggaatat gcggagaatt
     4621 tggttttaat cagccgccgg agtagggaat aatccgcatc gtcgggtggc agcgtccaaa
     4681 tggcgtgaat gtggttcggc agcacgcata cggcgacggt ttcaaaggga tattgttttt
     4741 gcacatccat ataagctgcc cgcgaagtcg atatgttcga caagcaggcg cgatttcggg
     4801 tcggcgagtt tgacggtgaa aaagaatgtg ccgccggcaa tgaagtttct gcgataacgc
     4861 gccataggat ttttgtttgt ggtttgggtg ttggagaagg aatggtttgt tgtttaaggt
     4921 tttaactttg ttggagctga tgttttcaga cgacattgga ggccgtctga aagcgctggc
     4981 ttcaacgaag ttaaaagttg gaacggcaaa ccgttaatca accaaccgcg tacgtaccgc
     5041 acacacactg cacgttggct ttgaagtttc gtagactcac aggtaggtgt ggcgcaacca
     5101 cgcatacggt gggttgggta taaaggctac aacttgctat agcttgcttg ctattcaaaa
     5161 taagaatgct taattgctgt tgctaattga gcagcaagga tgggaggaac ggcattacca
     5221 acctgcgtga attgccgcgc aaccgctcct gtaaaaatat aatcatcagg aaacgtttgt
     5281 aatcgtgcac tttcacgaac agataacgtt cttgggatat atggatgtaa gtgagacctt
     5341 cctccacctt tagtgccacc agcaataata gttttagatg gtttgcttgg atctaaccta
     5401 tctacacgtc cctttttatc acgttgccca taagctaatt ccatatagcg taaaatcgat
     5461 tcagccgaat gttctcttgt aatatggtta tgttggttaa taattggaat ttcaaatgta
     5521 ttaccacatg ccaatggggt attggttggt tgtagcggac taaatttttt atctcctaaa
     5581 taaccaatta taaatagcct aattctctgt tgaggtacat gaaaatattc agaattaatt
     5641 atttgaggtt tatagagtga gtagccttgc tgctgtaaat cacaatatac actagctaac
     5701 tgtttaccat tatctatatc catcaatcca gagacatttt caataagaaa tactttagga
     5761 cgaaaaatct taatcaattc aacgtaatta aataatagat taccattttg cttatgctca
     5821 aagccgacac gtttaaaatt tttatcagtt tttgagaatc tctggtttga ggctatggag
     5881 aaaggctgac agggaggacc tccaataaat acaccatcaa aacttaaaga cccaatatta
     5941 tttctgatta gttcaaccat ttcttctgtt tcagaaacat cagcattaaa cacagaccaa
     6001 ttagggcgat taagacgcaa tgtatcacaa aataaaggta ttttttcaat cagcatagaa
     6061 tgttgaaagc ctgctgcttc aaaccctaag tctaatccac cggctcctga aaaaaaacta
     6121 attatcggaa tgtgattttc ctttttttga aaatcactta gaaccaaatt ctttctcttt
     6181 tcaaaaggct ttttatcatg atgaatataa attaaatcat catagctcat ttgattctgg
     6241 cgtattagtt cattcaatgg aataccttgt aataaacttt gttctaataa ttttctttgt
     6301 gaaataattc tattattttc ttggtcaaat aaagaggttt gcggtattaa catagatact
     6361 cctatctatt tataaattct tttaacttta cttttagctt cttcatatac atgatatgct
     6421 tcttcataac taacaaaatc ttttttcttg tgcaaattaa gccaaaaagt atagtacttg
     6481 tgtgtggcac gatgacaggt tggacagata ccaaccaaat cttttaatga agtagtttta
     6541 tcttctaccc ttacaggaga agatagtggt aatagatggt gtacttcaat gatatgttcg
     6601 ctccattcat agctcctttg cgtatctaat ccacacatgc gacatatttg tgcattttgg
     6661 gtcttgctaa aatagatttt tcttaaaata ctattacgtt ctgtaattaa atgggtgact
     6721 ttaattttac caccctcact aaacgcctga tcatcaatag ataatgaaac gatttcattc
     6781 atcaataaga aattattatt tgtcttaccc aatctcatta tctctaaact atctaatctt
     6841 tcttgtggtt gtgtatctgc aataaattgg ttttctaata aatgtagttc actaaaatct
     6901 gtaatatcta aaaacaactt attttcatac caagacaaaa aagagaattg actaataaac
     6961 tttaccaact ctctaatttg acgaggatca tatttaattg cttctttatt cataattaac
     7021 tgtgagaatg aaaaagaagc aatacctgta atactattat tactagcgat tttgtaaata
     7081 atttcatcca tgtccaaaaa attaactcct gtttccttat attttgaaat taaagctcta
     7141 ataattgcca aaacagggaa aacccgattt tcagatggtt gatatcctga aaaagctggt
     7201 gatgggtagg aaaaatgtgt tgcaaaatgt agtaaataat catcgcaact aatttcatca
     7261 ttctgaacta aatcggcaca taaatctgta gtacagatta acccattatg ctccgttgcg
     7321 agtaacaaac atttaaaaac acgaccataa ttacgccaaa ctggatattg tgaactgtta
     7381 ggggcaaaag gtaagtttgt ctcttgagtc aggcttaatc ttacaatatc aatatctttt
     7441 gcacgtggct ttggacattg attaattcca tataatcctt ttgcaatttt tttaatttca
     7501 tcaaat
//
LOCUS       DAWKQJ010000094         7488 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000094 DAWKQJ010000000
VERSION     DAWKQJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.094"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            162..1505
                     /locus_tag="WJL20_001416"
     CDS             162..1505
                     /locus_tag="WJL20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ7683566.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1508..2740
                     /locus_tag="WJL20_001417"
     CDS             1508..2740
                     /locus_tag="WJL20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ7683567.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2733..3509
                     /locus_tag="WJL20_001418"
     CDS             2733..3509
                     /locus_tag="WJL20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ7683568.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARVAGKDPT
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3509..4135
                     /locus_tag="WJL20_001419"
     CDS             3509..4135
                     /locus_tag="WJL20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ7683569.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4139..4732
                     /gene="nqrE"
                     /locus_tag="WJL20_001420"
     CDS             4139..4732
                     /gene="nqrE"
                     /locus_tag="WJL20_001420"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ7683570.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4746..5963
                     /gene="nqrF"
                     /locus_tag="WJL20_001421"
     CDS             4746..5963
                     /gene="nqrF"
                     /locus_tag="WJL20_001421"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ7683571.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6115..7170
                     /locus_tag="WJL20_001422"
     CDS             6115..7170
                     /locus_tag="WJL20_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7683572.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7196..7411
                     /gene="nqrM"
                     /locus_tag="WJL20_001423"
     CDS             7196..7411
                     /gene="nqrM"
                     /locus_tag="WJL20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ7683573.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
BASE COUNT         1830 a         2036 c         1889 g         1733 t
ORIGIN      
        1 cagcttccgc aaggaaaaaa gcagcaaatc tgacaaacag gtacaattcc tgatagaatc
       61 taacactttg ctacattaat ttattcaatg cttttgatgc cacgcgacac ggtatcaaaa
      121 cacgccgttt ttaccttaac catcacaagt aatgcaaata tatgattaaa atcaaaaaag
      181 gtctaaacct gcccatcgcg ggcagaccgg agcaagtcat ttatgacggg cccgtcatta
      241 ccgaagtcgc gttgcttggc gaagaatatg ccggtatgcg cccctcgatg aaagtcaagg
      301 aaggcgatgc cgtcaaaaaa ggccaagtgc tgtttgaaga caaaaagaat ccgggcgtgg
      361 tgtttactgc gccggcttca ggcaaaatcg ccgcgattca ccgtggcgaa aagcgcgtac
      421 ttcagtcagt cgtgattgcc gttgaaggca acgacgaaat cgagtttgaa cgctacgcac
      481 ctgaagcgct ggcaaactta agcggcgaag aagtgcgccg caacctgatc caatccggtt
      541 tgtggactgc gctgcgcacc cgtccgttca gcaaaattcc tgccgtcgat gccgagccgt
      601 tcgccatctt cgtcaatgcg atggacacca atccgctggc tgccgaccct acggtcatta
      661 tcaaagaagc cgccgaggat ttcaaacgcg gcctgttggt attgagccgt ttgaccgaac
      721 gcaaaatcca tgtttgtaag gcagctggcg cagacgtgcc gtctgaaaat gctgccaaca
      781 tcgaaacaca tgaattcggc ggcccgcatc cggccggttt gagtggcacg cacattcatt
      841 tcattgagcc ggtcggtgcg aacaaaaccg tttggaccat caattatcaa gacgtgattg
      901 ccatcggacg tttgtttgca acaggccgtc tgaacaccgg gcgcgtgatt gctttgggtg
      961 gttctcaagt caacaaacca cgcctcttgc gtaccgtttt gggtgcgaaa gtatcgcaaa
     1021 ttactgcggg cgaattggtt gacgcagaca accgcgtgat ttccggttcg gtattgaacg
     1081 gcgcgattgc acaaggcgcg cacgattatt tgggacgcta ccacaatcag atttccgtta
     1141 tcgaagaagg ccgcagcaaa gagctgttcg gctgggttgc gccgcagccg gacaaatact
     1201 ccatcacgcg tacaaccctc ggccatttcc tgaaaaacaa actcttcaag ttcaacacag
     1261 ccgtcaacgg cggcgaccgc gccatggtgc cgattggtac ttacgagcgc gtgatgccct
     1321 tggatatcct gcccaccctg cttttgcgcg atttaatcgt cggcgatacc gacagcgcgc
     1381 aggcattggg ttgcttggaa ttggacgaag aagacctcgc tttgtgcagc ttcgtctgcc
     1441 cgggcaaata cgaatacggc ccgctgttgc gcaaagtgct ggaaaccatt gagaaggaag
     1501 gctgattatg ggcttgaaac attttctgga aaaaatcgaa ccgcacttcc tgccgggcgg
     1561 caaacatgaa aaatggtatg ccctctacga agcggcggcg acgattttct acacatccgg
     1621 tgcggtaacg cgcaaagcgg cacacgtccg cgacgcgctc gactccaagc gcatgatgat
     1681 tttggtgtgg ctggctttgt tccccgccat gttttacggc atgtacaacg ttggcgcaca
     1741 ggcattcggt gccttaacgc ccgatttgct gcaacaaagc atcgccaacg actggcatta
     1801 cacccttgcc gacgctttgg gcatcaatat gtcgtctgaa gcgggtgtgt cgggcaaaat
     1861 gctgttcggc gcgatttact ttctgccgat ttacgcgacc gtatttgtcg tgggcggttt
     1921 ttgggaagtc ttgttcgcca ccgtgcgcaa acacgaaatc aacgaaggtt tcttcgtgac
     1981 ttcgattctg tttgccttaa tcgttccgcc cacgctgccg ctgtggcagg ctgccttggg
     2041 tattaccttc ggcgtggtgg ttgcaaaaga ggtattcggc ggtacgggta aaaactttat
     2101 gaaccctgcg ctggcaggcc gcgccttcct gttcttcgcc taccctgcca acttgagcgg
     2161 cgatgcggtt tggacggcgg ttgacggcta ttccggcgca accgcgctgg cgcaatgggc
     2221 ggcacacggt gcagacggtc tgaaaaacgc cgtaaccggt caaaccatca cttggatgga
     2281 cgcgtttatc ggcaaactgc ccggctccat cggcgaagtc tccactttgg cactcttaat
     2341 cggcggcgcg tttatcgtgt ttgcccgcat cgcttcttgg cgcattattg ccggcgtgat
     2401 gatcggtatg attgcgatgt cttcgctgtt caacttcatc ggttcggaca ccaacgctat
     2461 gtttgctatg ccttggtact ggcatctggt cgtcggcggc ttcgccatcg gtatgctgtt
     2521 tatggcaacc gaccctgttt ccgcctcctt taccaatgtc ggcaaatggt ggtacggcgc
     2581 gcttatcggc gtgatgtgcg tattgatccg cgtggtcaac cctgcttacc ccgaaggcat
     2641 gatgttggcg attctgtttg ccaacctgtt tgccccgatt ttcgactatt ttgtcgcaca
     2701 agcgaacatc aaacgcagaa aggcgcgcag caatggctaa gaaattcgat aaagacagct
     2761 tcagcggcac gctgattgtc gtgttggcgg tcagcctgat ttgctcggtc atcgttgccg
     2821 gtgcggtcgt cggcttgaaa cccatccaag agaaacaaaa actccaagac aaacaaggct
     2881 atatcttgag cgtagccggt ttgatggata aggacaccga catcggtaaa acctttgccg
     2941 agcgtatcga gcaacgtgtc gtggacttgg cgaccggcga atatgtgaaa gacgcgccga
     3001 aagacttcag cgcgcgcgtt gccggcaaag accctaccca aagcatccaa atcaaacctg
     3061 aagacgattt ggcaggcatc aaaagccgtg ccaaatacac cgaagtttac ttggtaaaag
     3121 gcgaagacgg caaaatcggg caaatcatcc tgcctatgca cggcaacggt ttgtggtcgg
     3181 taatgtacgg ttttgtcgcc atccaacccg acggcaacac catcaacggc atcacttatt
     3241 acgaacaagg cgaaaccccg ggcttgggcg gcgaaatcgg caatcctttg tggcagcaaa
     3301 aattcgtcgg caaaaaactg tttgacggac aaggcaaact cgccctgcac gtcggcaaag
     3361 gcgcgggttc ggacaaagaa cacggcgtag atgccctctc cggtgcatcg ctgacatcca
     3421 aaggcgtgca aggttcgttc gcctactggt tcggcgaaaa cggctatatc ccctacctga
     3481 acaaattgaa atcagcagga gcgcaataat ggctgatatg aaacgcttga aacatttgat
     3541 gttttcaccc tttatcgaca acaacccgat tgccttgcag gttttgggta tttgttcggc
     3601 tttggcggtt accaccaaac ttcagacggc catcgtgatg ggtatttccg tagctttggt
     3661 aactggtttt tccagcttct tcatttcgct ggtacgcaac tacatcccca acagcatccg
     3721 tattatcgtg caaatggcga ttatcgcgtc gctggttacg ctggtcgacc aactcttgca
     3781 ggcatttgcc tacgaattgt ccaaacagct ttccgtattc gtcggtctga ttattaccaa
     3841 ctgtatcgtg atgggccgcg ccgaagcatt tgcgatgaaa gagccgccgc tggaaagcct
     3901 gatcgacggc atcggcaacg gcgcgggcta cgggataatg ctgcttgtcg tcgccaccgt
     3961 ccgcgaactg attggctcgg gcaaactctt gggctacacc gttttccaaa ccgtgcagga
     4021 cggcggctgg tatcagacca acggcttgtt cctgctcgcc cccagcgcgt tcttcatcat
     4081 cggctttttg atttggggac tgcgtacctg gaaacccgaa caggcggagg aataagctat
     4141 ggaacactat ttgagcctct tcatcaaatc cgtcttcatt gaaaatatgg cactatcctt
     4201 ctttttgggt atgtgcacgt ttttggcggt atccaaaaaa gtatccaccg cattcggttt
     4261 gggtgtggcg gtaattttcg tactcgggct gtccgtccct gtcaaccaac tcgtttactc
     4321 gctgctcaaa gacggcgcga ttgtcgaagg cgtggatttg acctttttga aattcatcac
     4381 cttcatcggc gtgattgccg ctttggtgca gattttggaa atgttcttgg ataaattcgt
     4441 ccccgccctc tataacgcgt tgggcatcta cctgccgctg attaccgtga actgcgcgat
     4501 tttcggcgcc gtatcgttta tggcgcaacg cgaatacaac ttcggcgagt ccgttgtata
     4561 cggcttcggc gcgggcttgg gctggatgtt ggcgattgtc gctttggcgg gcattaccga
     4621 aaaaatgaaa tattcggacg ctcccaaagg cctcaaaggc ttgggcatta cctttatcgc
     4681 cgccggcctg atggcgatgg cgtttatgtc gttctccggc atccagttat aagaaaggga
     4741 ccggtatgga gattatttta ggtatcgtga tgtttaccgt catcgttttg gttttggcac
     4801 tgatgattct atttgccaaa tccaaactgg tcagcgaagg cgacatcacc atcaaagtca
     4861 acggcgaaaa agagctgacg atgcccgccg gcggcaaact cttgggcgcg cttgccaacg
     4921 aaggcatctt tatcccctcc gcctgcggcg ggggcggttc gtgcggacaa tgccgcgtcg
     4981 tcgtaaaaag cggcggcggc gacatcctgc cgaccgagct gtcccacatc agcaaacgcg
     5041 aagcacgcga aggctgccgt ctgtcgtgcc aagtcagcgt caaaaccgac atggacatcg
     5101 aagtgcccga agaagtgttc ggcgtgaaaa aatgggaatg caccgtcatc tccaacgata
     5161 acaaagccac gttcattaaa gaactcaagc ttgccattcc cgaaggcgaa gaagtcccct
     5221 tccgcgccgg cggctacatt caaattgaag ctcctcctca cactgttgcc tacaaagact
     5281 tcgacattcc taaggaatat cacgaagact gggacaaata caatttgtgg caatacgttt
     5341 ccaaagtgga cgagccgatt ttgcgcgcct actctatggc ttcataccct gaagaaaaag
     5401 gcatcattat gctgaacgtg cgtatcgcca cgccgccccc acgcgtacct gatgcgcctc
     5461 cgggacaaat gtcgtcctac atctggtcgc tcaaacccgg tgataaagta acgatttccg
     5521 gtccgtttgg cgaattcttt gccaaagata ccgatgccga aatggtattt atcggcggcg
     5581 gcgcgggtat ggctccgatg cgttcccaca ttttcgacca gctgaaacgt ttgaactcca
     5641 aacgcaaaat taccttctgg tatggtgcac gttccaaacg cgagatgttc tatgtcgaag
     5701 acttcgacca actcgcggca gaattcccga acttcacgtg gcacgtcgcc ctgtccgatc
     5761 cgctgcctga agacaactgg gacggttaca caggcttcat tcacaacgtg gtttacgaaa
     5821 accacctgaa aaatcacgaa gcgccggaag actgcgaatt ttatatgtgc ggcccgccga
     5881 ttatgaacca gtccgtcatc aaaatgctca aagacttggg tgtggaagac gaaaacatcc
     5941 tcttggacga tttcggcggt taaatattcg gcttcaaaca caaaaggaag aaacctcggt
     6001 ttcttccttt ttttatccga taccgtccga acacctttca gacggcatcg gcatagaaac
     6061 ataccggccg ttttgcgata taattcaaac catttgccaa aaccgtcaaa cactatgccg
     6121 tctgaaacac gcctgccgaa ctttatccgc accttgatat ttgccctgag ttttatcttc
     6181 ctgaacgcct gttcggaaca aaccgcgcaa accgttaccc tgcaaggtga aacgatgggc
     6241 acgacctata ccgtcaaata cctttcaaac aatcgggaca aactcccctc ccctgccgaa
     6301 atacaaaagc gcatcgatga cgcgcttaaa gaagtcaacc ggcagatgtc cacctatcag
     6361 cctaattccg aaatcagccg gttcaaccaa accacggccg gcaaacccct ccgcatttca
     6421 agcgacttcg cacacgttac tgccgaagct gtccgcctga accgcctgac gcacggcgca
     6481 ctggacgtaa ccgtcggccc cttggtcaac ctttggggat tcggccccga caaatccgtt
     6541 acccgtgaac cgtcgccgga acaaatcaaa caggcggcat cttatacggg catagacaaa
     6601 atcattttga aacaaggcaa agattacgct tccttgagca aaacccaccc caaggcctat
     6661 ttggatttat cttcgattgc caaaggcttc ggcgttgata aagttgcggg cgaactggaa
     6721 aaatacggca ttcaaaatta tctggtcgaa atcggcggcg agttgcacgg caaaggcaaa
     6781 aacgcgcgcg gcgaaccgtg gcgcatcggt atcgagcagc ccaatatcgt ccaaggcggc
     6841 aatacgcaga ttatcgtccc gctgaacaac cgttcgcttg ccacttccgg cgattaccgt
     6901 attttccacg tcgataaaaa cggcaaacgc ctctcccata tcatcaaccc gaacaacaaa
     6961 cgacccatca gccacaacct cgcctccatc agcgtggtcg cagacagtgc gatgacggcg
     7021 gacggcttgt ccacaggatt attcgtattg ggcgaaaccg aagccttaaa gctggcagag
     7081 cgcgaaaaac tcgctgtttt cctgattgtc agggataaag gcggctaccg caccgccatg
     7141 tcttccgaat ttgaaaaact gctccgctaa acatacaaac cgattaggaa acaccatgaa
     7201 aaccctgctc cttaccttcg gcatcttcct gaccgtcatc atcggtatgg cggtcggcta
     7261 tattttctcc aaacgcagca tcaaaggcag ttgcggcggc attactgcct tgggtatgaa
     7321 aaaaatgtgc gactgcgaca caccttgcga caccctgcaa aagaaactgg atgaagaaaa
     7381 acaggcaggc ggcatacggg ttgaccgtta acgccgaaat atagtggatt aacaaaaacc
     7441 agtacagcgt tgcctcgcct tagctcaaag agaacgattc tctaaggt
//
LOCUS       DAWKQJ010000095         7474 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000095 DAWKQJ010000000
VERSION     DAWKQJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.095"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..480)
                     /locus_tag="WJL20_001424"
     CDS             complement(58..480)
                     /locus_tag="WJL20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ7683574.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            611..3361
                     /gene="secA"
                     /locus_tag="WJL20_001425"
     CDS             611..3361
                     /gene="secA"
                     /locus_tag="WJL20_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ7683575.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQIILSEAGHEH
                     AEQILTKMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPEHGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNTIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            3505..5277
                     /locus_tag="WJL20_001426"
     CDS             3505..5277
                     /locus_tag="WJL20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7683576.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKTQFEDALLNQSKPLSEYFWEHLSDDLNLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVIPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            5464..7392
                     /gene="rpoD"
                     /locus_tag="WJL20_001427"
     CDS             5464..7392
                     /gene="rpoD"
                     /locus_tag="WJL20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ7683577.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2089 a         2159 c         1792 g         1434 t
ORIGIN      
        1 ccattatcct ttacttctta atattaacgg atgtttacac aaattcccgt atacatttta
       61 tgcgccatgc cttctgacca agtttgccaa tgcctccgcc aattcgggat gccgtttttc
      121 caactttacc gccgccgaac cgaaactctc cagcgcagcc ttactcaaat gcagggtatt
      181 ggttttcggc ggtttttccg gtttcgggac cagcctgacc gaaacagagc gtatcgaagc
      241 atcaagccct gccaactgcg gcaataccga cggtgcaatc attttcaagc gcgatgccgc
      301 catattgttt gccgccaaaa ggacaagcct gccgtcttcg atacatgccg tctgaaaatg
      361 cgggtgcagg ttggcaggca gcagtttttt cacggcggca tccaaccgcc gccactgtcc
      421 cgcctgtttc aaaagtccgg aaagcagcgc gtcccgcctg cccaactgtt ccaaattcat
      481 aaaacataca cccaaaaaga ttgaaatacc gcaaacgcgc ctttatttca gacggcatta
      541 gcactttgca caaacgcttg tgttaaaatc gcgttttcgc ccactattat atcaggcgca
      601 ggaattattc atgctgacaa acattgccaa gaaaatcttc ggcagccgca acgaccgctt
      661 gctgaaacaa taccgtaaat ccgttgccag aatcaacgcg ctcgaagaac agatgcaagc
      721 cctaagcgat gctgatctgc aagccaaaac tgccgaattc aaacaacgcc tcgccgacgg
      781 tcagactttg gacggcattt tgcccgaagc cttcgccgtc tgccgcgaag cgtcccgccg
      841 caccctcggt atgcgccact tcgacgtgca gcttatcggc ggtatggtgc tgcacgacgg
      901 caaaatcgcc gaaatgcgta ccggcgaagg caaaaccctg gtcgccaccc tcgccgtcta
      961 tctcaacgcg ctggccggca aaggcgtaca cgtcgttacc gtcaacgact acctcgcctc
     1021 acgcgatgcg ggcattatgg agccgctcta caatttcctc ggccttaccg tgggcgtgat
     1081 tatttcagat atgcagccgt tcgaccgtca aaacgcctat gccgccgata tcacctacgg
     1141 caccaataat gaattcggct tcgactacct gcgcgacaat atggttaccg accaatacga
     1201 caaagtgcag cgcgaattga attttgccgt tgtcgatgaa gtggattcca tcttgattga
     1261 cgaagcgcgc actccgctga ttatctccgg tcaggcggat gacaacatcc agttgtacca
     1321 aatcatgaac accgttccgc cccacctcgt ccgtcaagag acagaagaag gcgaaggcga
     1381 ctattgggta gatgaaaagg cgcaccagat catcctgagc gaagccggcc acgaacacgc
     1441 cgagcaaatc ctgaccaaaa tgggattgct ggcagaaaac gactcgctct attccgccgc
     1501 caacatcgcc ctgatgcacc accttatggc ggcattgcgc gcgcattccc tcttccacaa
     1561 agaccaacat tacgtcatcc aagacggcga aatcgtcatc gtggacgaat ttacaggtcg
     1621 tctgatgtcc ggccgccgct ggtcggaggg tctgcatcaa gccgtcgaag ccaaagaagg
     1681 cgtggaaatc aaacgcgaaa accaaaccct cgcctccatc accttccaaa actatttccg
     1741 cctgtacacc aagctctccg gcatgaccgg cacagccgat accgaagcct tcgagttcca
     1801 aagcatctac aacctcgaaa ccgtcatcat cccgaccaac cgccccgtac agcgcaaaga
     1861 cttcaacgac cagattttcc gttccgccga agaaaaattc gaagccgtcg ttaaagacat
     1921 tgaggaatgc cacaaacgcg ggcagcctgt tttggtcggc acgaccagca tcgaaaactc
     1981 cgaactggtc tcccaccttc tgcaaaaagc cggactgccg cacaacgtcc tcaacgccaa
     2041 agaacacgaa cgcgaagccc tgattgtcgc ccaagcaggc aaagtcggcg cgattaccgt
     2101 tgccaccaat atggcgggac gcggtacgga catcgtattg ggcggcaacc tgaagcacca
     2161 aaccgatgcc atccgtgccg acgaaacctt gagcgacgaa gagaaacagg cacaaatcgc
     2221 cgcactcgaa aacggctggc aggcagaaca cgacaaagtg atggaagcag gcggtttgca
     2281 catcatcggt acggaacgcc acgaaagccg ccgtatcgac aaccagttgc gcggacgctc
     2341 cggccgtcag ggcgaccccg gatccagccg tttctacctc tcgtttgaag acccgctgct
     2401 gcgcctattc gcactcgacc gcgccgccgc catcctcaac cgtctcgccc ccgaacacgg
     2461 cgtcgccatc gaacacaacc tgctgacacg ccaaatcgaa ggggcgcaac gcaaagtcga
     2521 aggcagaaac ttcgatatgc gcaaacaggt tttggaatac gacgacgttg ccaacgaaca
     2581 gcgcaaagtc atttacagcc agcgcaacga aattctgacc agcaaagaca tcagcgacct
     2641 gatgaaggaa atccgttctg atgtcgtcag cgacctcgtg gatacctata tgccgcccga
     2701 cagcatggaa gaacaatggg acatcccgac tttagagaac cgtctggctg ccgaattcag
     2761 actgcacgaa gacattcaat cctggctgaa agcagacaat acaattgacg gtcaagacat
     2821 caaagagcgc ctgatcgaac gcatcgaaaa cgaatatgcc gccaaaaccg aactggtcgg
     2881 taagcaggca atggccgatt tcgagcgcaa cgtgatgttg caggtcatcg acaaccaatg
     2941 gcgcgaacac ctcgccgcta tggactacct gcgacaaggc atccaccttc gcagctatgc
     3001 ccaaaaaaat ccgaagcagg aatataaacg tgaagccttt accatgttcc aagacctgtg
     3061 gaacggcatc aaattccata ttgcctccct gcttacctcg gttcaaatcg aacaaaaccc
     3121 tgtcgcggtg gttgaagagc aacccatcgg caacatccag tccatccatt ccgaatcgcc
     3181 cgatatggaa gaacttttgg gtcagtcgca aaccgatttg gttaccgagg cctttaatcc
     3241 cgatggaacg gatttcagcc ccgaagcctt ggaagcgcgg ggacaaatcg tccaccgcaa
     3301 cgacccctgc ccctgcggca gcggtttgaa atacaaacaa tgccacggca aactggcttg
     3361 agcgtttgaa cgcaaatgcc gtctgaacat ccggtttccg gcttcagacg gcattttgcc
     3421 tgaaccgcca catccgaccg ccattccgaa aaatcccgat ttcgtaccgt ccaaactaaa
     3481 aacaggcatc cgccccgccg aactatgatt ccatccgact tcattgacga acttctggcc
     3541 aaaaccgata ttgtcgacat catcgacgaa caggttccgc tgaaaaaagg cggggcgaac
     3601 tatatggcgt gttgcccgtt ccacaaggaa aaaacgccgt cgttttcggt cagtccgacc
     3661 aagcagtttt accactgttt cagttgcggg gcgcacggtt cggcgattgg ttttgtgatg
     3721 gaacatcagg ggctgtcgtt tccggaggca gtgcagttcc ttgccgaccg cgtgggtatg
     3781 attgtgccta aagtgcgcgg gcagaacgat aatcccgaag tccgtgccga acgtaagaaa
     3841 aagcagcaga cgctggaaga aacgacagcg gcggcggctg atttttatgc gcaacagttg
     3901 aaattcaatc cgacggcgaa ggcttatttg gacaagcgcg gcttgagcgc ggaagtcatc
     3961 gcgcattatg gtttgggcta cgcgcccgac ggctggcagc ctttggcgca agtattccaa
     4021 ccgtacccga ataccgcgtt ggtggatacg ggtatggtga ttgacaatga ggggcggtat
     4081 tacgaccgat tccgtcatcg gattatgttc cccatccgca atccgcgcgg gcaggtcatc
     4141 ggtttcggcg gcagggtgct ggacgactcc aaacccaaat atctaaactc tcccgatacg
     4201 cctttgtttg ataaggggaa aaacctttac ggattgtatg aagggcgtgc tgctgtcaag
     4261 gaagtggggc ggattctggt ggtcgaaggc tatatggacg tggtcgcgct ggcacagttc
     4321 ggcgtgggct acggcgtggc ggccttgggc acggcaacaa cagcggaaca cgtcaaaatc
     4381 ctgatgcgtc aggcggacag tatttatttc tgtttcgacg gcgacagcgc ggggcgcaaa
     4441 gcggcttggc gcgcgctgga aaacgcgttg ccgcagttga aagacgacaa atcgctgcat
     4501 tttttgttcc tgcccgagga acacgacccc gacagttaca tccgtgccta cggcaagacg
     4561 caatttgaag acgcgctgtt gaatcaaagc aagccgctgt cggaatattt ctgggaacac
     4621 ctttcagacg acctcaatct caatacgcag gaaggcaagg cggaattggt gaaaaccagt
     4681 tcgccgcttt tggcgcaaat caccgcgccg gcattggctt atttgttaaa acaacggctt
     4741 agcgaactgg tcggcatcga tcctgacaat ctcgcgcaac tgctgggaca ggaagcgccg
     4801 aagcggcacg tcaaacaaaa aaactacaaa ctgcccccaa tttccgtcaa acagcccgtt
     4861 ataccgacat tggtgcaacg gcaaatccgc agcctcttga taaatccgga ttgggctgca
     4921 tatatagacc tgcccgatta tctggcgttg gacggtgatt tcgcctgcct tgccaatctt
     4981 gccgaatcga ttaaaaacca tgccgccgta cccgaaaccg ctcaggtttt agagtatatg
     5041 cgcggctcgc cttacgaaga aacgataacc cgaatcttcc attcaacgca ccaatcggaa
     5101 gaaatgaaca gcagcagtga agaagattgc gagaatttcc aaatcggcat gaaaaaactg
     5161 ctcaatgagt taaaatacag ccaaatcgaa gcattgaaac aaaaaagcct gcaatccggc
     5221 ttaaatgaaa gcgagaaaaa acttttgctg tcgttgctga ccgcaaaaca aaattgaccg
     5281 gcggattccg ccacccgtaa acccgttatg ccgtctgaaa agcacttacc ccggctgcaa
     5341 caacgacacc tgcagaacac ccatcccaaa aagccttcag acggcatcag agtacccgct
     5401 ctgccacgcc ttcaggtgcg ttcaaacgca aaccgtcggc accttaccaa cagaaagcag
     5461 acaatgtcca gaaaccaaaa tcacgaagaa tatcaagacg acacccgtcc gttaagcatt
     5521 gaagagcaac gcgcgcgcct gcgtcagctc atcatcatgg gtaaagaacg cggctacatc
     5581 acctactccg aaatcaacga cgccctgcca gacgatatgt ctgatgccga ccaaatagac
     5641 aatatcgtca gcatgatttc cggtttgggc atccaagtta ccgaacacgc ccccgatgcg
     5701 gaagacatat tgttaagcga caatgccgcc gttaccgacg atgatgccgt cgaagaagcc
     5761 gaggccgccc tttccagtgc agattccgag ttcggcagaa ccaccgaccc cgtccgtatg
     5821 tatatgcgcg aaatggggca ggtcgacctg ctgacccgcg aagacgaaat catcatcgca
     5881 aaaaaaattg aaaacgccct gaaaaatatg gttcaggcca tctccgcctg cccgggatcc
     5941 attgcggaaa tcttagaact catcgaaaaa atccgcaaag acgaaatccg cgtcgacgaa
     6001 gtcgtagaag ccattatcga cccgaatgaa gtattgctca acgaattggg cttggggcac
     6061 ttggaaacca cagcgcccga gaaaccttcc aacgacaatt cggacgaaaa cgaagacgac
     6121 gaagaatcgg aagaagatgc ggatgaaatc tcggcagcca atctcgccga attgaaacaa
     6181 aaagtcatcg gccactttgc ccaaatcgaa aaagactaca aaaaaatgat tggccgtttg
     6241 gaaaaacacc acagccggca caaagactat ctcgcctacc gcgacgcgat tgccaacaaa
     6301 ctgctggaag tccgtttcgc cacccggcaa atcgacagcc tcagcagcag cctgcgcggg
     6361 aaagtagaaa acatccgcaa actcgaacgc gaaatccgcg acatctgcct cgaccgcgtc
     6421 catatggagc gcgactactt catccaaaac ttcctgcccg aaatcaccaa tctggaatgg
     6481 attgaagaag aaatcgccaa aggcagggtt tggagcgacg cgctcgaccg cttccgccac
     6541 gccatcctcg aaaaacaaac cgagttggcg gatatggaaa aagaaacccg catttccatc
     6601 gaagagttga aagaaatcaa caaaaatatg gtgtcgagcg aaaaagaaac cgcagccgcc
     6661 aaacaggaaa tgattcaggc aaacttgcgc ctcgtgattt ccatcgccaa aaaatatacc
     6721 aaccggggct tacaattcct tgatttgatt caggaaggca acatcggttt gatgaaagcg
     6781 gtcgataagt tcgaataccg cagaggctat aaattctcca cctacgcaac ctggtggatc
     6841 cgccaggcaa ttacacgctc gattgccgat caggcgcgta ccatccgcat tccggtacat
     6901 atgattgaaa ccatcaacaa gatgaaccgc atctcgcgcc aacaccttca agaaaccggc
     6961 gaagaacccg attccgccaa acttgccgaa ctgatgcaga tgcccgaaga caaaatccgc
     7021 aaaatcatga aaatcgccaa agagccgatt tcgatggaaa cccccatcgg cgacgacgac
     7081 gattcgcact tgggcgactt catcgaagat gccaacaatg ttgcgccggc cgatgcggca
     7141 atgtacacca gcctgcacga agtaaccaaa gaaatactcg aaagcctgac cccgcgtgag
     7201 gcaaaagtcc tgcgtatgcg tttcggcatc gatatgaaca ccgaccacac gctggaagaa
     7261 gtcggcagac agtttgacgt aacgcgcgaa cgcatccgac aaatcgaggc aaaagcactc
     7321 cgcaagctgc ggcatccgac aagaagcgac cgtttgagaa gtttcttgga cagcgaagac
     7381 agcaagctgt aaaccaaaaa accgcaggtt tcaaatacct gcggtttttt cttacacaat
     7441 aaacaacgct tccacatatc ccacactcct atcc
//
LOCUS       DAWKQJ010000096         7470 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000096 DAWKQJ010000000
VERSION     DAWKQJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.096"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            140..721
                     /locus_tag="WJL20_001428"
     CDS             140..721
                     /locus_tag="WJL20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7683578.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="WJL20_001429"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="WJL20_001429"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ7683579.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="WJL20_001430"
     tRNA            1860..1937
                     /locus_tag="WJL20_001430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="WJL20_001431"
     tRNA            1962..2038
                     /locus_tag="WJL20_001431"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="WJL20_001432"
     tRNA            2066..2141
                     /locus_tag="WJL20_001432"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2277..2783
                     /locus_tag="WJL20_001433"
     CDS             2277..2783
                     /locus_tag="WJL20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ7683580.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2780..4558
                     /locus_tag="WJL20_001434"
     CDS             2780..4558
                     /locus_tag="WJL20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ7683581.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4671..5495
                     /locus_tag="WJL20_001435"
     CDS             4671..5495
                     /locus_tag="WJL20_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ7683582.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6257..7045)
                     /locus_tag="WJL20_001436"
     CDS             complement(6257..7045)
                     /locus_tag="WJL20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ7683583.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7259..7453)
                     /gene="thiS"
                     /locus_tag="WJL20_001437"
     CDS             complement(7259..7453)
                     /gene="thiS"
                     /locus_tag="WJL20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ7683584.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1594 a         1928 c         2237 g         1711 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 ttttgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaaa atcatgagaa tccgcgtgtt
      481 gaaaaccgac ggccgcaatc ccggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ctttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aattccacgt cgccgcacag gctgccgtcg tccaaacggt tgatgcccac atcgataacg
      961 accgcgccgg gtttgatcca ttcgcctttg acaaagttcg gaatgcccac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgtc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag cagttccaaa gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagggg cagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ttcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggca gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc ctccgaccaa aaccacggcc aggcaaggca tgtgcagatt gtcctgttgg
     1561 cgttgcgcca ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatattga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt cttttcccgt ttgcgcgtac tgtccgcgcc gccggctttg cctttcgcat
     2221 atgcttctgt atcatgtggg tttttctgtc ttttatggtt tttgtcggag gctttgatgg
     2281 ttgacgcttg gtctgtccct gattttgaat cgaaaatctg tccgcccgag gcgttggcgg
     2341 cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa cggctgtttc gacatcctcc
     2401 acagggggca tgttacttat ctggcgcagg cgcgttcgat ggggggcgcg ttggtgctgg
     2461 cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg cggcgaccgc ccgattaatc
     2521 ctttggagaa ccgtgccgcc gttgccgccg cgttggaaag tgtggatttg gtaacgtggt
     2581 ttgacgggga tacgccggcg gcgttgattg aggcggtcaa acctgaaatt ttggtcaagg
     2641 gcggcgattg ggctgcggat aagattgtcg gtgcggcaga aacgttggcg cgcggcggtc
     2701 aggtgttttc aattccgttt ctgcaccaga cttcgacaac gaagactttg gcaaaaatcc
     2761 gtgcggcaga gggcggaaaa tgacggtttt gaagccttcg cactggcggg tgttggcgga
     2821 gcttgccgac ggtttgccgc aacacgtctc gcaactggcg cgtatggcgg atatgaagcc
     2881 gcagcagctc aacggttttt ggcagcagat gccggcgcac atacgcgggc tgttgcgcca
     2941 acacgacggc tattggcggc tggtgcgccc attggcggtt ttcgatgccg aaggtttgcg
     3001 cgagctgggg gaaaggtcgg gttttcagac ggcattgaag cacgagtgcg cgtccagcaa
     3061 cgacgagata ctggaattgg cgcggattgc gccggacaag gcgcacaaaa ccatatgtgt
     3121 gacccacctg caaagtaagg gcagggggcg gcaggggcgg aagtggtcgc accgtttggg
     3181 cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg ccgcagtatg agttgggttc
     3241 gctgtcgcct gttgcggcag tggcgtgccg gcgcgccttg tcgcgtttgg gtttgaaaac
     3301 gcaaatcaag tggccaaacg atttggtcgt cggacgcgac aaattgggcg gcattctgat
     3361 tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc ggtatcggca tcaatttcgt
     3421 gctgcccaag gaagtggaaa acgccgcttc cgtgcaatcg ctgtttcaga cggcatcgcg
     3481 gcggggaaat gccgatgccg ccgtgttgct ggaaacgctg ttggtggaac tggacgcggt
     3541 gttgttgcaa tatgcgcggg acggatttgc gccttttgtg gcggaatatc aggctgccaa
     3601 ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc gaaaccgtgt tcgaaggcac
     3661 ggttaaaggc gtggacggac aaggcgttct gcacttggaa acggcagagg gcaaacagac
     3721 ggtcgtcagc ggcgaaatca gcctgcggcc cgacgacaga ccggtttccg tgccgaagcg
     3781 gcgggattcg gaacgttttc tgctgttgga cggcggcaac agccggctca agtgggcgtg
     3841 ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg taccgcgatt tgtcgccttt
     3901 gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc atcgtcggtt gcgctgtgtg
     3961 cggagaattc aaaaaggcac aagtgcagga acagctcgcc cgaaaaatcg agtggctgcc
     4021 gtcttcggca caggctttgg gcatacgcaa ccactaccgc caccccgaag aacacggttc
     4081 cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt
     4141 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt atctcggggg
     4201 aaccatcatg cctggtttcc acctgatgaa agaatcgctc gccgtccgaa ccgccaacct
     4261 caaccggcac gccggtaagc gttatccttt cccgaccaca acgggcaatg ccgtcgccag
     4321 cggcatgatg gatgcggttt gcggctcggt tatgatgatg cacgggcgtt tgaaagaaaa
     4381 aaccggggcg ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg caaaagttgc
     4441 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg acaacctcgt
     4501 cattcacggg ctgctgaacc tgattgccgc cgaaggcggg gaatcggaac atacttaaaa
     4561 aatacaccct ttttcaacac ggggcggcat ccgtccgaat ccgtccggat tctgccgccc
     4621 gacagactgc cgcccaggcg ggcggggttt caaacattga ggaaattcta atgaaatggc
     4681 tcttcaccct tttggcggta ctcaatattg ccgtattcgg cggcacggtg ggctataaat
     4741 tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc cgtcgaaaac acgccccccg
     4801 caacgcccgc cgccgacaat gcggcggcat ccgtggagga tacggcggca ctgctcaaac
     4861 cgggcgacat cctgaccgaa gagcaggccg agcagttgcg cttgaaaaag gaagcggaac
     4921 agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc ccgccgcgaa aaactcgccg
     4981 ccgaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga tgccttatgc gccgcgcagg
     5041 caagcctcac gatggacgaa gatgactacc accgcatcaa agggcttttg ggcaaatggt
     5101 cgcacgttgc cagcaggagc gttgaaaaac gcaccgccaa agccaaacct gccgacaaaa
     5161 cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa tcaggcggcg gagctgtctg
     5221 ccaaaggttt caaccccata ccgtttgacg gcgcattgag tttgggtgtc ggcaacagtc
     5281 gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc cggattcggc ggggcgcata
     5341 ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc tttgagcgtg tcgcgtatga
     5401 cggttttgtt taccggcgtg aatgccgccg atgcggacga aatccgtaaa atcacgtccc
     5461 tatacggcaa actgaacctc aagtcttgca aataggcgcg aaagccggac gataaacgcc
     5521 aaacccaccg cgaaggtggg tttggcgttt cggttttgag tatctgatat ataaaaaggg
     5581 aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg atgatgggga aatatggtgt
     5641 ttaatggttc ggaacggacg gcttccgagt ggttttgatt atacgctaat aataataatt
     5701 atcaataaaa atttaaaaat aaatgcagat tttttgtaat acgatgaaaa ataaacaaat
     5761 taacctgtcc gccgatgttt gtgattcggg tcggcaaact ttcattgtgt cggacaaatc
     5821 ggggtcggca ggcatagctg agggcttcgt gcctgacgga aataagcctg ccgtgtttga
     5881 acatccgtcc gatttggcgc ggacagggtt gtcattcggg ggtgtttgag cgtggattcg
     5941 gggatgcaaa agcggtaaaa ccgttgcgga tgaaggctta cggacggctt taggtatttt
     6001 taagtgtatt caaacaacaa caaaacgctt cttctattgc cgtatttagg gcttgcgcgg
     6061 cagcctgcac accgtggagg ccgagtgttt cgttggttgc ggcatcggga cgggcggcaa
     6121 ggttttccgg ccggtttttc cattttttcc gacagagccg tgatgcttgg gaatggcggt
     6181 ttgcccgttt cagaccgccg ccgttttggg ctgcgatgcc gtctgaaccg ttcagacggc
     6241 atttttgccg cctttttcaa tattccgccg aatgccaaaa cggctgtccg actgtcggcg
     6301 tgctggcttg cgctttgtcg cgtgcttcga ccggtccggc ttcaaatgcc agccgtccgg
     6361 attcgacggc gagtgcgaag gcgcgtgcca tattgaccgg atcgccgctg cgggaaacgg
     6421 cagtattcaa aagcacgccg tcaaaacccc attccatcac ttgtgccgcc tgtgagggca
     6481 aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag gcgttcgcgc aggacgttca
     6541 acgcgtaggc gtgaaccgcg cccaaacccg tgccgatcgg agccgcccac ggcattaacg
     6601 cctgacagcc cacatcgagc aggcggcggc aggcaatcag gtcttcggtg caataaggca
     6661 gcactttgaa gccgtcttta atcaggattt ccgccgcttc gacaagttgg aacacatccg
     6721 gctgcaaggt gtcgtcgtcg ccgatgagtt cgagtttaat ccaatcggtt tcaaacactt
     6781 cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct ttggcagcct gccgtgttcg
     6841 gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa cccctgaccg tgcgcctcgc
     6901 cgccgcttcc cgtgcggcgc agcgagacgg taatcatcgc gggttgggcg atacggacgg
     6961 attgtttgag gatttcaggg gtcgggtagg cagccgtgcc gagcagcagc cgtgaaggga
     7021 aagtttctcc gtatagggtg agcataatgg gttcctttgt atagtggatt aacaaaaacc
     7081 agtacggcgt tgcctcgcct tagctgaaag agaacgattc tctaaggtgc tgaagcacta
     7141 agtgaatcgg ttccgtacta tccgtactgt ctgcggcttc gtcgccttgt cctgatttaa
     7201 agttaatcca ccataacgtt gttttgggga caggggtcgt ctgaaaaggc aaaaccgcct
     7261 agccgccgac caccggccgc acgatatcga ttttgtcgtt ttcgtttaaa accgtttccg
     7321 catacgcgcc tttggggacg aaaacggtgt tgaccgccac ggcaaagggc ttttgcggcg
     7381 cggtttgggc gatgaggtcg gcaacgcttg tgccgtgaag ttcggcgggt ccgccgttta
     7441 agatgatgtt catggtttct cgatgtttct
//
LOCUS       DAWKQJ010000097         7452 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000097 DAWKQJ010000000
VERSION     DAWKQJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.097"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            154..327
                     /locus_tag="WJL20_001438"
     CDS             154..327
                     /locus_tag="WJL20_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7683585.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVNRDAVGVI
                     RKEAGKSLSQPEK"
     gene            391..1674
                     /gene="hemL"
                     /locus_tag="WJL20_001439"
     CDS             391..1674
                     /gene="hemL"
                     /locus_tag="WJL20_001439"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ7683586.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1890..2162)
                     /locus_tag="WJL20_001440"
     CDS             complement(1890..2162)
                     /locus_tag="WJL20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ7683587.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2173..2736)
                     /gene="orn"
                     /locus_tag="WJL20_001441"
     CDS             complement(2173..2736)
                     /gene="orn"
                     /locus_tag="WJL20_001441"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ7683588.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERTTP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="WJL20_001442"
     CDS             complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="WJL20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ7683589.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETIEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(3759..5120)
                     /gene="accC"
                     /locus_tag="WJL20_001443"
     CDS             complement(3759..5120)
                     /gene="accC"
                     /locus_tag="WJL20_001443"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ7683590.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5233..5688)
                     /locus_tag="WJL20_001444"
     CDS             complement(5233..5688)
                     /locus_tag="WJL20_001444"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ7683591.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5670..5927)
                     /locus_tag="WJL20_001445"
     CDS             complement(5670..5927)
                     /locus_tag="WJL20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ7683592.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6044..7084
                     /gene="queA"
                     /locus_tag="WJL20_001446"
     CDS             6044..7084
                     /gene="queA"
                     /locus_tag="WJL20_001446"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ7683593.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1546 a         1877 c         2222 g         1807 t
ORIGIN      
        1 acggaagagg gaatcgcaca aacggttgtt aaaggcaaaa cgacccgggc ggaggtagag
       61 gcacgtttcg ggaagcgcaa ccctttcggt tgttatgcct atcatgaagt cagcctgccg
      121 atttataatt ttttgccgac caatttcatt tatatgaaat cagaacgccg gcattgggag
      181 tggtgtgtgg attacgacgg ggagggagtt gtccgggact accgctttac acataaagtg
      241 gaaaaagacg aacgttccgt caaccgggat gcggttggcg tgatccgcaa agaagcgggc
      301 aaatccttgt cgcaacctga aaaatgataa aatggggctt tctgcttcca agcccgaaac
      361 ctgccgttca ggcggcattt gaggataaat atgaaccgta acgaaatttt attcgaccgc
      421 gccaaggcgg tcatccccgg cggtgtgaat tcgccagtcc gcgcattcgg cagcgtcggc
      481 ggcgtaccgc gcttcatcaa aaaagccgaa ggcgcgtatg tttgggatga aaacggcacg
      541 cgctacaccg actatgtcgg ctcgtggggg cctgcgattg tcggccacgc gcaccccgaa
      601 gtcatcgaag ccgtgcgcga agccgcgttg ggcggtttgt cgttcggcgc gcctaccgag
      661 ggcgaaatcg tcatcgccga agaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
      721 ctcgtcagct ccggtaccga agcgaccatg accgccatcc gtctggcacg cggctttacc
      781 ggccgtgaca aaatcatcaa atttgaaggc tgctaccacg gccattccga cagcctgttg
      841 gtgaaagcag gcagcggtct gctcaccttc ggtaatccct cttccgccgg tgtgcctgcc
      901 gactttacca aacacacttt agtcctcgaa tacaacaaca tcgcccaact cgaagaagcc
      961 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
     1021 aatctggtta ggccgtctga agccttcgtc aaagccctgc gcgaattgac cgaaaaacac
     1081 ggcgcagtgt tgatttacga cgaagtgatg accggtttcc gcgtcgcgct cggcggcgca
     1141 caatcgctgc acggcatcac gcccgacctg accacgatgg gcaaagtcat cggcggcggt
     1201 atgccacttg ccgcgttcgg cggacgcaaa gacattatgg aatgtatttc cccgctgggc
     1261 ggcgtgtatc aggcaggcac cttgtcgggc aacccgattg ccgtcgccgc cggtttgaaa
     1321 acgctggaaa tcatccgccg cgaagggttt tatgaaaacc tgacggcgcg taccgaacag
     1381 ttggttcaag gttttcggac ggcagcggat gcggcgggca tcgagttcac cgccgacagc
     1441 gtgggcggta tgttcggtct gtatttcgcc gcacacgcgc cgcgaaacta tgccgatatg
     1501 gcgcgttcca atattgacgc tttcaaacgc ttcttccacg gtatgctcga ccgcggcatt
     1561 gccttcggcc cgtccgctta tgaagcaggt ttcgtttctg ccgcgcatac gcccgagctg
     1621 attgacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctgc atgatgtttt
     1681 gacggacaga gtttctctgt tcgatttgtt tggcagattg aagtaagaat gcacaccgcc
     1741 gtcattcccg cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1801 ggccgaaatc agccgcgccc ggcaggaagc cgccgccacc tgaaacaatg ccgtctgaag
     1861 ccttcagacg gcattttatc ggaagcgcat cagaaaaaac ggatagcgac atatgcggta
     1921 aaagcaaggg caattacata gataaacccc tttataaatt caaaagtctc atctatatcg
     1981 ctatattttt ctttcagttc gtcagatttt cgataacgtt tcattttcaa gaccacttca
     2041 tgaccaacaa gcccgacctt ccggattccc gcctgcgcgg gaatgacggg cgtgtatatt
     2101 tttgatttca atctgctgta aaaatgccgt ctgaaatgca ggatttgcgc ttcagacggc
     2161 attttttgtt tcttattgcg cttcggctct cggggcggaa atcagaaagt gttcgcggta
     2221 gtggcgcatt tcttcgatgc tctccaaaat gtcgtccaat gccttgtgcg aaccgcgttt
     2281 gaccacgctt ttggcaacgg gcggattcca gcgtttggca agctctttga gcgtggaaac
     2341 gtcgaggttg cggtagtgga agtagttttc cagtttcggc atatatttga ccataaaacg
     2401 ccggtcttgg tggatggaat tgccgcacat cggcgtggtg cgttcgggta cccattccga
     2461 cataaagtcc agcagtttct gttcgacttc ggcttcggta tgcgacgatt cgcgtacgcg
     2521 ctgcgtcagc cccgtcctgc cgtgtgtggc ggtgttccac tcgtccatat tgtcgagcag
     2581 ctcgtcactt tggtggacgg cgtaaacttc ggattgcgcc aatacattca aatccgagtc
     2641 ggtaataatc atcgcgactt cgataatgcg gtcggtttcg ggattaagcc ccgtcatttc
     2701 catatcgagc cagcagaggt tgtttttatc ctgcacggtg tttcctttcc gtttcagcgt
     2761 tttacgccgc tcaatcgcgc ccatccttct tcggtttccg cagggtcgag gtcgaaccat
     2821 tggctgtaaa tgccgccgag ttcttcggct tgttcgtcca acaaaccgga taacacgatg
     2881 cggccgcctt gtttggtgcg ggcggcgagc atttcgccga gcatacgtaa agggttggcg
     2941 aggatgttgg cgacaactac gtcgaattgc ccttgaggca gaccgtcggg caggaagaat
     3001 tgtgcatcga cgttgttctg ttcggcgttg tccttgccgg cgcggacggc ttgttcgtca
     3061 atatccacgc cgacggcaga acctgcgccg agtttgaggg cggcgatggt caggatgccc
     3121 gaaccgcagc cgtagtcgag gacgctttcg ccgtttttga gttgcgtatc cagccatttg
     3181 aggcagaggc gcgtggtcgg gtggctgccg gtgccgaagg cgaggccggg atcgaggcgt
     3241 aggttgacgg cagtgccttc gggggcttcg tgccaagagg gggtaatcca caggcgttcg
     3301 gaaattcgga tggggtcgaa ttgcgcttgg gtcaggcgta cccagtcttg gtcttcgatg
     3361 gtttcgccgg tgtatgccaa gtcttttaac ccgcattctt gtgcggcggt ttggatgatg
     3421 gcggcggctt cgtcgtgttc gccgaacagg gcgatgactt tgctctgctg ccagatttgt
     3481 tcggcgggca taccgggttc gccgaaaatc gcctgttcgt tttgcgtacc ggcgtaggca
     3541 tcttcgatgg cggcggagag tgcgccgtgt tccatcagcg cgtcggcgag gcgttcggcg
     3601 acggcatcgt tgacgttgac ggtgatttgt tggtagggca tagcgggctt tcttaacaga
     3661 ccttcggaat agggtcgtac tgtataaggg gatgggtgtg taacggaaat gccgtctgaa
     3721 aacggacaaa ctgtttcaga cggcatatcg gggacggctt atttgtcctg tttggctttg
     3781 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
     3841 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
     3901 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
     3961 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
     4021 acgcggatgc cgaagccgcc gggcaggtgg cagctttcaa tcggccccgg gctggggatg
     4081 aagttgtacg ggtcttcggc gttgatacgg cactcaaacg cgtggccttc gacttgaata
     4141 tccttttgtt tgtattgcaa cggcaggccg gcggcgatgc gcaattgctc ttgtacgatg
     4201 tccacgccgg tgatgagttc ggtaaccgga tgctcgacct gaacgcgcgt gttcatctcg
     4261 ataaagaaaa attcgccgtc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
     4321 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttc ttcagtgatg
     4381 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
     4441 tcggcaagat atacggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
     4501 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
     4561 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
     4621 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     4681 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     4741 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     4801 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     4861 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     4921 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     4981 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     5041 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     5101 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     5161 aaaggtgcgg gaacgctgaa gggggaaagc atttcagacg gcatcggaag tttatgccgt
     5221 ctgaaaacag gattatccga tgatgaagag cggttcgccg aattcgacgg gcgtaccgtt
     5281 ttcgaccaaa atttctttga ccgtgccgga tttttcggct tcgatttcgt tcatcagctt
     5341 catcgcttcg atgatgcaca gcgtgtcgcc ggctttaacc tgttggccga cttcgacaaa
     5401 aggcgcggca ttcgggccgg gtgcgcggta gaacgtgccg accataggtg atttttgcgc
     5461 gtcggacaaa tcgcgggcgg caggtgcggc ggcaggcgcg gatgccgcaa cgggtgcggc
     5521 ggcaggcgtt acggccggcg cggcggcagg tacgggcgcg gcgtaaacgg gtgcggcggc
     5581 gatggttcgg gtgatgcgga ctttttcctc gccttcggtt acttcgattt cggcgatacc
     5641 cgattcttca accaaatcaa tcagtttttt taatttgcgc aaatccattt ctgttccttt
     5701 aaaggctgcc ggtgttccgg cgggttgttg cgttggtttt tcagaaaaca gccggcttgc
     5761 ggcaacggcg caaaccgggc ggaatggtgt tcggatacgg ttttcctgtg gtggaaaaca
     5821 accgtgagaa tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa aatatcaaaa
     5881 aacggcggtt tttgggggaa atgtgcagga agtttgattt tgcgcacaag atgttccagg
     5941 ttaagagtgt ttattctaat ctgttggttt ttcggacaaa gatgccgtct gaaaaagggc
     6001 taaagtgcgt ataatggcgg cttgcccgaa cgagagtgta aaaatggata tttcagattt
     6061 tgattttacc ctgcccgaaa agctgattgc ccagcatccg cccgaagtgc gcggcagcag
     6121 ccgccttttg gttgcgttgc ccgatatgcc gctgcaagac cgggtgttcg gcgatttgcc
     6181 ggattatgtc gaggcgggcg acgttttggt gttcaacaac accaaagtca tgaaggcgcg
     6241 gctgtttggg cagaaagaca gcggcggcag gatcgaagcc ctgattgagc gcgtgttgga
     6301 caaccatacc gcactggcgc acatccgttc gtccaagtcc cccaagcccg gtatggggct
     6361 ggtgtttgaa ggcggcatcc gtgccgtgat ggtcgggcgt gagggcgaac tgttctgcct
     6421 gcgttttgaa ggcggtcaaa ccgtttacga acttttggaa cagaacggac acctgcccct
     6481 gccgccttat atcgaacgcg ccgccgacgc ggacgacgac agccgttatc aaaccgttta
     6541 tgccaaatat cagggtgcgg tcgccgcgcc gacggcgggt ctgcatttta cggaagaact
     6601 tttgcgccgc ctgaaagaca aaggcgcagt aaccgcagaa gtaaccctgc acgtcggtgc
     6661 gggaacgttc cagcccgtgc gtgtggaaaa aatcgaagaa cacaaaatgc acagcgaatg
     6721 gtttgaagtg ccgtctgaaa ccgccgccgc cgttgaggcg gcaaaagcac gggggaacaa
     6781 agtctgggcg gtcggcacga cttccatgcg cgccctcgaa tctgccgcgc gtgcgacggg
     6841 tcgtctgaaa gcggggcagg gcgacaccga tattttcatc acgccgggct accgttttaa
     6901 tgttgtcgac aggctggtta ccaattttca tttgccgaaa tcgacgctgc tgatgcttgt
     6961 cagcgcgttt tcgggtatgg gtcatatccg cgccgcgtat cgttatgcgg tcgaacgcga
     7021 ataccgtttc ttcagctacg gcgatgcgat ggttttgggg cggaacgaag gggtggtacg
     7081 ttagaacagc cccaagtcta ttgggggaag acaagtgccg tctgaacgga cgttcggacg
     7141 gcattctttt gttgatttga atgaaaggaa ctgtatgaca tagattcagg cgcgagaggc
     7201 gagaaactca gtcattgttg atgacgcgca atacgttaaa attcattccg ttttggtcgt
     7261 ataaacatat tacatacgcc atttttttgt attcaaggct gcctgaaaac agtgggcaag
     7321 aatatctaac ccaacgaaaa gctgaaaaag caacaaaaaa taatctgcac cttagtcggt
     7381 tggttagtcc aaccttaggg gtgcagacca ttcaatcttt caggctgcct ttgtttgttt
     7441 ataagataaa tt
//
LOCUS       DAWKQJ010000098         7253 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000098 DAWKQJ010000000
VERSION     DAWKQJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.098"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..990)
                     /locus_tag="WJL20_001447"
     CDS             complement(<1..990)
                     /locus_tag="WJL20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ7683594.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGY"
     gene            complement(1040..1270)
                     /locus_tag="WJL20_001448"
                     /pseudo
     CDS             complement(1040..1270)
                     /locus_tag="WJL20_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(1320..4325)
                     /locus_tag="WJL20_001449"
     CDS             complement(1320..4325)
                     /locus_tag="WJL20_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ7683595.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDYNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSRLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTAKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EGLERIFKKKNLAEVGQEEMQANIAILETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            complement(4439..5749)
                     /locus_tag="WJL20_001450"
     CDS             complement(4439..5749)
                     /locus_tag="WJL20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ7683596.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPIKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            6061..6483
                     /locus_tag="WJL20_001451"
     CDS             6061..6483
                     /locus_tag="WJL20_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683597.1"
                     /translation="MEWVSLFSDPQKDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADPQLGYTPELSVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVSDGDGVQDVASGKKYRQTIVP"
     gene            6569..7253
                     /locus_tag="WJL20_001452"
                     /pseudo
     CDS             6569..7253
                     /locus_tag="WJL20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT         1767 a         1602 c         1830 g         2054 t
ORIGIN      
        1 ataaccataa cttgataaat ctatttgatt tatgatttgg taaaacaaat agtttggcat
       61 tcgttcatta ttgcttaata ttacttgtgt accatctgct ccccgcgcat attgaaaatt
      121 aactaactta acaattcttg tatgatcacc aaaaataata tgagcatctt gtgggtttaa
      181 tatagatttt tcatcattgg taaatccaca aataaaatct tgactttggt caacaacagg
      241 atattttcca aaatccttaa tatctttatt taatattttt gtagtatttg ggatttttgc
      301 taagcaagat tgtaattcta tacttcccca ccccttcgga atttcccgtt tcaaggtttc
      361 gtcaaacacc atgtcgccgc ctgaggattt gtagggcttg ccgttggcgt cggggaaatc
      421 gaactgcaca aaccagtagt cgtacagggt tttcgccatc tcttccaggc gcgcgttgat
      481 ttgtttgttc agggcgattt ttttgtccaa agcggatagg acggcggcga tggattgttg
      541 gatttgaggt tctggaatag gcaactccag tgtttttaat gcattttcat tgacacgttg
      601 cctgccggaa gttccttcca tacattcaat cgctcgtttt ctaaaatcgg gactgattgc
      661 aaaataatag agaaattcgg gattggtttc attttttgct cttaacacga taaattcggt
      721 agaaccgaaa gccacttctc catcatctaa aatatctaca aaagcggttt tcccattttc
      781 caaacacggg gtaatcttag ctaataaggt atcaccattg cgaaacttag ctccaccgtt
      841 aaatgccttg atttcgtaac ccgtaatttg tcgttgaaat tctttcaaca ttgccatcgg
      901 aacacttttt gccaatgctc ccttggctag ttgctctctc ggattaaaat cagcaatatt
      961 ttgaatttgt tgtttcttaa aatgattcat gatgttccgt tacaaaactc ttcaaaggcc
     1021 gtctgaaaaa aacatttcat cggtagttct tgccgtctgc ggcagttgta aactgaaagt
     1081 ttacaactga atcgtccagt tcttgttctg caaatatggc tttgatgtgt ttaccgatgt
     1141 tttgtttgct ggtttggtag agttcggcaa tttctttttg ggtaagccat agttcgccgt
     1201 caagctcgcg cagggtaaat tcggatagtc cgtcttccgt ggtgtagatg atgatgtcgt
     1261 tgttgtgcat agctttcctg tatttataaa tggttaaggc tacctaaaaa acctctttct
     1321 caaatccgcc cgctttcttt taaatattcc cgcaccaaca ggcggttgat ggcttggatg
     1381 tctgcaggct gaacaggaaa ttgctgttcc gttctaaagc gttttaatac gatgccctgc
     1441 atttgttttt caaagtaatt ttggttgtcc aaaatctgct ccatatccag cactttttgg
     1501 tccgtgtcgg tttttacacc attcagcgcg ttaaagactt tgagtttatc gccgtagagg
     1561 gcggggtttt cgcgcaggcg tttgtggatg cgggcatatt tggcgtcgcc gccgtatttg
     1621 tgccgcaaga ggtcgttttg gcggttcagc tctttgattt tggcatagac ggtttcgagt
     1681 atggcgatgt ttgcctgcat ttcttcttgc ccgacttcgg cgaggttctt tttcttgaag
     1741 atgcgttcca gtccttcgcg caggctgatg tattcggggt cttcttgatc gaagttgccc
     1801 gccagccctt cgcggacttt gcgcatgatg tctttcaaat cgtcggcaag tttgagttcg
     1861 gcttcgccga ttttaacgaa ttgaaagtaa acgtcttcca gcgcttcatt gagcaaatgg
     1921 gcggtgtcgc cttgctgtag tttttcggca aggtttaagg tctccaaacg cgcggcggtt
     1981 tcgcggtaca aaagattgag cttgtcaaaa tcgagatggg cgagaaattc gtggctgcct
     2041 tgcaggcgca ggacgttgta cagctttttg gcggtttgca gggctttttt tagggcgagc
     2101 agctgttttt tgtctgctat ttggctaatt tggctgcaaa attcttcggc gttttcggta
     2161 tcgaaatcaa acagggcgtt tttaatgtct gcaatttcct gttcgatttc ttcggcggtt
     2221 ttgaaaagcc ggctgtagct gccgatttcg tcgcccaatt cgttggaaag ttcgtcccaa
     2281 taggcgcggt tggttttgtc aaattcgcgc tcgatgtcgg caaaatcgac aacatagccg
     2341 tagcggtaag atttgtaggt gcggttgacg cgggtcaggg tttgtaaaag gttatgggct
     2401 tttatcagtc tgcccaaata gagctttttc aggcgcggcg cgtcaaagcc ggtcaaaagc
     2461 atgttgtaca caaacaagat gtcgattttg cccgctttga aatctttaac ccattggtcg
     2521 cgttcgtcct tagtgccgac gtcgtgcaat atcagcgcgg cggtgaaatt atagtctgag
     2581 gctatctgaa aatgctcaaa aagctgacgt gcctgttccg cgctgtcgca aacaaccata
     2641 gcgccgaggc tgtcgtcttg gttggttttt ctgaatttgg cgaagtcgtc caagatgtag
     2701 tcaagcatcg ggtggacgaa gtgcggatgg gcgtagattt ctttgcggtc aaagctgcct
     2761 ttttcgattt caagttgcgc cagtgcttct tgtaattgcg ccttgtatcg gctgccgatt
     2821 tcttcgcgta tcaggcgcag ggtgtagccg tcggcaatgg aggcgttgta atagtatttg
     2881 tggatgtagt cgccgaagag ttcgcgggtg ttgacgttgc ctgcggttac gccgataagc
     2941 ggcgtgccgg tcagcccgat tttgacggcg tttacgtcgg actggttgag gttggcaaga
     3001 aacgagcctt tggggttgta gctgcggtgt acttcgtcga gaaaataaac gcgctggatg
     3061 gcgaggtcgt agtcgttacg ggcgacgaca tcggggtcgt cttggaattt ttggatgttg
     3121 acgacagtga tttccgcctt gcctgcgtgg ttgtgcaaag tttgggcgga tttgatgtcg
     3181 gcagcaaagg cttcgcggct gtcgatggta tggacgacta aatcgcgggc agtaaattcg
     3241 cgctgcgcct gtttcaataa atcaagcctg tccacgatga aatagaattt cggcacgatg
     3301 ccctgtttgg cataatagtg ggtcaggtag cgggtgttgt aataggcaag cgcggttttg
     3361 ccagagcctt gggtgtgcca aatcacgcct tttttgccgc cgtttgccaa atgtttttca
     3421 atggcgagag tggcaaatag ctgcggatac cgcatgatgt gtttttgcac caagccttga
     3481 cttgctttga cgtaagtcag cccgtgttgc agaagaaaag aaagacgttc gcggcaaagc
     3541 agcgaagtca ggatgcggtt ggtcggcgta tcgggcgatt tattgctgat aaattcgggg
     3601 ctgtgtttga taacgggcag gttgttgtct tgcaggacgt tgttttcgag gccgtctgaa
     3661 agcgtgttca ataattcggc aagatttaat ttatgttctt cgcggaagta gttaaacacg
     3721 ggcttaccgt aagcagacga agcgtaaaac gcgccttgtg ccggttcggt tgcgccgtcg
     3781 tcgtattcca tgttgttgga aaaaatcatg aattgggtga tgttgatgaa acggcgaaat
     3841 ttggaatttt tcgcacgttt gcccatgcgc tcccgctctt cgccgattcc gcctttgttg
     3901 ttgggctttt tgacttcgat aaaaaccaaa ggcatgccgt ttaccagcag ggtaatgtcg
     3961 gggcggaatt cttcatcgcc gttgatacag ggcaattcgg ttacaacgtg gaagctgttg
     4021 ttatcaaaat tttggaagtc gatcagtttc ttgccgccgg attgattggt aaggcgttcg
     4081 taaaactttt gccccaagtc ttcgttgtcc agttctaagc gtatgtcggt cagcaaacgg
     4141 cgtgcatcgt ccggcgacaa atcgggattg atgcggcaga ggctttctat aaagattttg
     4201 ggaaagatgt tggttggttt atcccaattt tcgtttttga gcgagaggta gtcgtagccc
     4261 aagcgcatca ggtgcaaaac ggcggggatt ttgacgcggg aattttcgtt gtgagtttgg
     4321 gacataaacg taatgattca tcaaaattta ttgaaacgga ttataacacc tgaaaaaccc
     4381 gatacgcctt ttcggtgtac cggctttgcc atactgttct gcttcagaca gcattgcttc
     4441 attttgcctt taatacttct tcgtccagcg atttcaacca ttccagcttt tcgccgattt
     4501 tgatttccaa cccgcgcggg acgggttggt agaagtccgg ttcgtccaaa ccgtcgggca
     4561 tatagctttc gccggcggcg taggcgttcg gttcgtcgtg ggcgtagcgg tattcgcgtc
     4621 cgtagcccaa ttccttcatc agcttgatcg gggcgttgcg caggtggacg ggcacttcgt
     4681 cgctggcgtt ttctttgacg aagtggcgca tttggttgta tgccttgtag cccgcgttgg
     4741 atttggcggc ggcagcaagg tacagcacgg cttgagccaa agccagttcg ccttcgggcg
     4801 agcctaagcg ttcgaaggtg gcggcggcat cgttggcgat ttggaaggcg cgcgggtcgg
     4861 caagcccgat gtcttcccaa gcgatacgca cgatgcggcg ggcgaggtag cgcgggtcgg
     4921 tgccgccgtc gagcatacgg cagaaccaat acagcgcggc gttcggatgc gaaccgcgca
     4981 cggatttgtg cagggcggag atttggttgt agaagctctc gccgcctttg tcgaaacggc
     5041 ggatttgcgc cccgagactg tcggcgagaa attcggcggt taagtttttc agacgacgtg
     5101 tatcggcggc gcgtaaaagt tgttccaaca aattcaacaa tctgcgcgca tcaccgtcgg
     5161 cggtattgac gaacagctcc tgcgcgtcgg cttcaatcgt gaaatcccgg tattcgggca
     5221 aagccaaaac tttggcaacc agctttttga ggccgtctga agacaagggc tgcaaaacat
     5281 acacctgcgc gcggctcaac agcgcgggat tgacttcaaa cgacggattt tccgtcgtcg
     5341 caccaataaa ggttagcaaa ccgctttcga catgcggcaa aaacgcgtcc tgctgcgcct
     5401 tgttgaagcg gtggacttca tcgacaaaca aaatcgtcgc gcgtccctgc tgcaaagcga
     5461 tttcggcttt atcgattgcc tcgcggatgt ccttcacgcc ggaaaacacg gcggaaacag
     5521 gcaaaaactg ggcattgaaa ctctgcgcca aaatccgcgc caacgtcgtc ttgcccacgc
     5581 ccggcggccc ccacagcaac atagaatgcg gcttgccgcc ttctaccgcc acgcgcaaag
     5641 gtttaccttc gccgatgagg tgttcctgcc ccaccacgtc gtcaagcgta tgcggacgca
     5701 atcgttcggc aagcggcgcg tcgggttctc gggcaaacaa atcggtcata acggctccgt
     5761 caacaggttt tcaaacaata tgattatacg gcagggaacg gcggcgtgcc gcatacggat
     5821 tccgcccctc cgtttgcctt aagccgatat taggcgcata ctggaaaaga cgagagactt
     5881 cacacaatat atccggcacg gagaccgatt ccgcaccggc atgacaatac ccaaatcagc
     5941 gtttcaatta aacattaagg agactaaaat agaaaatttg ctttatctac cattgctttg
     6001 ttgattcaat cggcattatg ttttgaggcg gaagcccatg aatatactaa tattcaagag
     6061 atggaatggg tgtctttatt ttctgatccg caaaaagacg atgatagtct tataaccctt
     6121 aaagatgaaa aaatcactgt aaaaaactat attgtgcctt ggtggaaaaa aggtgaaaac
     6181 tttagaaaat tagaacttat taccactggt aaaaatgata atggaaattt aacggtaaat
     6241 ggaatttacg gcatccaatc ttttgcagac ccgcaattag gatatacgcc tgaattatcg
     6301 gtgaaaagca ataatgatat tctatttaaa aataatctcc aatctgttga cgtgaagccc
     6361 cattccggaa atatccattt ggatgccaaa ggaaaaattc aatttaattt gaatgtttcc
     6421 gatggtgatg gggtacaaga tgtagccagt ggaaaaaaat accgacaaac aatagtgcct
     6481 tagtgcggta tgtcaacact atcagccccc cgatgaaaat gacaaaggca gcatatcctt
     6541 tcattcaaaa acaggcaatt tgttcaatat gaacgttaac gttcataaag acacacacca
     6601 tttcggtatt cacaacctca acaataccga tgttcatctg actgccgaag acagcgataa
     6661 tgtgattgag attaaagggg aactcgccga ccctatgccg acaggaaatg ttgagagtac
     6721 aagtttttta aggggcattg aaacggcttt taaatccaat accgtattaa cggggaataa
     6781 caataaaata tccatcgcca ctgagggcaa gggcttggga ttgggcgtaa atggtctcca
     6841 gtccaactcc ccctattccg gacacacgac gggcattttc atcaataatc aatcaagcga
     6901 ccttacccaa ccttcccttg atggcatcag caccatcaaa ctcaaagccg tcaagggaaa
     6961 taatgaagtt gatttaaatg taaaaaacca cgcctccgta aaaggcatta tcgcgtcgca
     7021 ttctgcaaaa gcaacgcttg aagccgaagg ggacaatatt gtccaggtta aaaatccgga
     7081 caccaatacg gctttaatcg atgctttgaa aaaacaatac cccaatgctt cgaaagaact
     7141 atacagggtg ggggttcaat ccacgactgg aacagtacgc atttcgccaa acggcaaaat
     7201 cgcattaacg gaattgaaaa cttttggaat tgtacaaaac ggcatttgcg tca
//
LOCUS       DAWKQJ010000099         7200 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000099 DAWKQJ010000000
VERSION     DAWKQJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.099"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(60..144)
                     /locus_tag="WJL20_001453"
     tRNA            complement(60..144)
                     /locus_tag="WJL20_001453"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Leu,seq:caa)
     gene            420..1073
                     /locus_tag="WJL20_001454"
     CDS             420..1073
                     /locus_tag="WJL20_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ7683598.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1152..1805
                     /locus_tag="WJL20_001455"
     CDS             1152..1805
                     /locus_tag="WJL20_001455"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ7683599.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1909..2808
                     /locus_tag="WJL20_001456"
     CDS             1909..2808
                     /locus_tag="WJL20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ7683600.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLDEINSNELILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2805..4094
                     /gene="hisD"
                     /locus_tag="WJL20_001457"
     CDS             2805..4094
                     /gene="hisD"
                     /locus_tag="WJL20_001457"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ7683601.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4140..5237
                     /locus_tag="WJL20_001458"
     CDS             4140..5237
                     /locus_tag="WJL20_001458"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ7683602.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5234..6151
                     /gene="hisB"
                     /locus_tag="WJL20_001459"
     CDS             5234..6151
                     /gene="hisB"
                     /locus_tag="WJL20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ7683603.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6246..7115
                     /locus_tag="WJL20_001460"
     CDS             6246..7115
                     /locus_tag="WJL20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7683604.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1884 a         2117 c         1720 g         1479 t
ORIGIN      
        1 gttttatcta tttcattaaa cagcaataga caaaaaaata accgctctaa aagcggttgt
       61 ggtgcccagg gtcggactcg aaccgacaca ccttgcggcg ggggattttg agtcccctgc
      121 gtctaccaat ttcgccacct gggctggtga agaagtcgtc attataatgg cttttgaaat
      181 tctgtaaacc tttttttgaa attattttat ctgtttttat tttatttttg attttaaata
      241 gaatttttat tattttaatc ttactgttct ttccgctcca aagattctgt atgattcggc
      301 aattcctgcc gtgcaaacaa cgtaaaaaaa tactacatta aatctgccaa acgcgttaag
      361 atggaaatat tcaaattccg tacgaatcag gttttgctat ttattcttgg gagattgtca
      421 tgttttccgt accgcgttcc tttttgccgg gcgttttcgt acttgccgcg cttgccgcct
      481 gcaaacctca agacaacagt gcggcgcaag ccgcttcttc aagtgcatcc gcgtcagctg
      541 cggaaaatgc ggcaaagccg caaacgcgcg gtacggatat gcgtaaggaa gacatcggcg
      601 gcgatttcac gctgaccgac ggcgaaggca agcctttcag cctgagcgat ttgaaaggca
      661 aggtcgtgat tctgtctttc ggctttacgc actgtcccga tgtctgcccg acagagcttt
      721 tgacgtacag cgacacgttg aagcagttgg gcgggcaggc taaggacgtg aaagtggtgt
      781 tcgtcagcat cgatccggaa cgcgacacgc ctgaaatcat cggcaagtat gccaaacagt
      841 tcaatccgga ctttatcggt ctgacggcaa cgggcgacca aaacctgccg gtcatcaagc
      901 agcaataccg cgtggtttct gccaaagtca atcaaaaaga cgacagcgaa aactatttgg
      961 tcgaccactc ttccggtgcg tatctcatcg acaaaaacgg tgaggttgcc attttctcgc
     1021 cttacggaag cgagccggaa acgattgctg ccgatgtaag gactctgctc tgataaaacc
     1081 gtatgccgtc tgcaccgtcg gcgcctattc agacggcatt attgtttcaa ccgacaaagg
     1141 acatccacac catgcaggat aatgctttga ccatcgcctt atccaagggg cgcatttttg
     1201 aggagacgct gccgctgctt gccgctgccg gcattgttcc gactgaagag cctgaaaaat
     1261 cgcgcaagct gattatcggg acgaaccatg aaaacatccg ccttgtcatt gtccgcgcaa
     1321 ccgatgtgcc gacttatgtc cgctacggcg cggcggactt cggcattgcg ggcaaagacg
     1381 tgctgatcga acacggcggc acggggcttt accggccttt ggatttggag attgccaagt
     1441 gccgcatgat ggttgctgtg cgtaaagggt ttgattacga agcagcttcg caacccggat
     1501 gccgtctgaa gattgccacg aagtatcctg aaatcgcggc atctcatttt gccggcaagg
     1561 gtgtccatgt ggacattatc aaactgtacg gctcgatgga acttgcgccg ctggtcggct
     1621 tgagcgatgc gattgtggac ttggtttcga cgggcaacac cttgaaggca aacggcttgg
     1681 aagcagtcga acacatcgtc gacatttcca gccgcctggt ggtcaacaag gctgctttga
     1741 aaacgaaata cgcgctgctg gagccgatta ttcaggcgtt cggcggcgca gtgaaggcga
     1801 agtaagcatt catttgaata aagatgcgtt ttcagacgac cctatccgtt cccgccgaca
     1861 ggtcgtctga aaatatcacc ggcagtaaac tgtataggag aagttaaaat ggttgcaaaa
     1921 ataaaaaaat tctcagattc aaccctttcc gttttgaata acggcgagcg tcggttttat
     1981 gtctattgtc tgaccgacct gaaaaaagac aaaatcctct acatcggcaa aggctgcggt
     2041 aatcgtatct tcgagcatga atgggttgct agtcgttcac aagacccagt ctccggcgag
     2101 attatcgatc ggaaactcaa agccatctcc aaatgcaaga aactcggtcg ctatatcatc
     2161 agctatcatc tgactgaagt cgaagcgctc gccgccgaat ctgccttaat tcattttgtt
     2221 aaatctgtct tgggtaaaaa actcaaaaat aaaattgccg ggcatggtcc gggtggtatt
     2281 agcgtagaag aactagatcg ccgctttgga ttctcttctc tcccacttga tgagatcaac
     2341 tccaacgagc taattctcgc catcaaaatc cacaatgctt tcgatttaga tactgacgaa
     2401 gaattagact accttttcga caaccaagac gatgccaacc tcaaatcgcg tacgttgggc
     2461 aactgggtta tcggtaaaga tgttgcttca aaagtgaaat acgttatcgg cgttcacacc
     2521 ggtctgcaaa acgctgttgt cagtgcatac gaagtggacg gttttgaaac aatggttgag
     2581 gaaaccaaaa acggtagaaa acaatcccgt taccgtttcc gcactacctc tcgtagcgaa
     2641 gaggtattag ccaaactcgg tctgcaacaa aaatgcctgc ccgaattgaa gtttggtagc
     2701 gggggagaaa aagcgtatat cagacccaaa acagagacag aaactgaaca agagaatatt
     2761 cagacgaccc ccaatccaaa aataaaaaag gaaaaaacca aatcatgaaa aaactcaaca
     2821 cccaatcgcc cgatttccaa gccggactca aagccctgct ggcttttgaa accgcgcaaa
     2881 accccgaaac cgaacgcatc gtcgccgaca tttgcgccga cgtgcaaaag cgcggcgatg
     2941 cggctttgat tgaatacacc aacaaattcg atcagacaaa cgctaaaagc atcgatgatt
     3001 taatactcac gcaagccgat ttgaacgcgg cgttcgagcg cattccgaac gacgttcaga
     3061 cggcattgca gaccgccgcc cgccgtgtcg aaagctacca ccaacgccaa aaaatggaat
     3121 cgtggagcta caccgatgaa gacggcacgc tgttgggaca acaaatcaca ccgcttgacc
     3181 gcgtcggcat ttacgtcccc ggcggcaagg cggcgtatcc gagttccgtc atcatgaacg
     3241 ccatgcccgc ccacgtcgca ggtgtgaaag aaatcatcat ggtcgtgccg acaccaaaag
     3301 gcgaacgcaa cgacatcgta cttgccgccg catacgtcgc cggcgtaacc aaagtcttca
     3361 ccgtcggcgg cgcgcaggcg gttgccgccc tcgcctacgg cacggaaacc atcccccaag
     3421 tcgataaaat caccggtccg ggcaacgcct tcgtcgccgc cgccaaacgc cgcgtgttcg
     3481 gcgtggtcgg catcgacatg gtggcggggc cgtctgaaat cctggtcatc gccgacggca
     3541 cgacacctgc cgattgggtg gcgatggatt tgttcagcca ggccgaacac gacgaaattg
     3601 cccaagccat cctcatcggc acgtcgcaag cgtatctcga cgaagtagaa gccgctatgg
     3661 accgcctgat cgaaactatg ccgcgccgcg acatcatcga agcctcgctc ggcaacaggg
     3721 gcgcgatgat actcgccaaa gacttggacg aagcctgcga aatcgccaac tacatttccc
     3781 ccgaacactt ggaactgtca gtcgaaaacc cgcaggaatg ggcgaaaaaa atccgccacg
     3841 ccggtgcgat tttcatggga cgctacaccg gcgaaagcct cggcgactac tgcgccggtc
     3901 caaaccatgt gttgcccacc agccgaaccg cccgcttttc ctcgcctttg gggacatatg
     3961 atttccaaaa acgctccagc ctgattcagg tttcggaaca gggcgcgcaa aaattaggcg
     4021 aaaccgccag cgtgctggca cacggcgaaa gcctgaccgc ccacgcccgc gcggcagagt
     4081 tccgtatgaa ataatgccga aacggcgtac aggcatattc caaccattaa ggaaacacga
     4141 tgaaatccgt ccgctccttc atccgcgacg acatacaagc tatgtcggca tatcagattg
     4201 ccgacgttcc gcccggcttt gccaaactcg attcgatgga aagtcccgtc cacccttttg
     4261 ccggacatga aacgctgttg caggaatggc aggcacggct tgccgccgcg cccatccatc
     4321 tttaccccaa tccctccggc agcggtttac aggaagcatt acgttcggcg ttcgacattc
     4381 ccgactgcgc cgacatcgcg ctgggcaacg gttcggacga actgatacag ttcatcacga
     4441 tgctgaccgc caaaccgggc gcggcaatgt tggcagccga acccagtttc gtcatgtacc
     4501 gccacaacgc cgcgctgtac ggcatggatt atgtcggcgt tccactgaac ggagatttca
     4561 ccctcaacct gcccgccgtc ctcgaagccg tcaggaaaca ccgccctgcc ctgaccttta
     4621 tcgcctaccc caacaacccc accggcgtat gcttcacgcg tgccgaaatc gaagccgtca
     4681 tcgaagcttc agacggcatc gtcgtcgtcg atgaagccta cggcgcattc aacggcgaca
     4741 gcttcctgcc gcaggcaggc aggattccca acctgatagt cttacgcacc ctcagcaaaa
     4801 tcggttttgc cggactgcgt atcggttatg cggcaggctg ccccgaagtc atcggcgaac
     4861 tgcaaaaaat cctgccgccc tacaatatga accaattgag cctgaccact gccaaactcg
     4921 ccctgcggca ctacggcatt atctctgcca acatcgacag cctgaaaaac gaacgcgaac
     4981 ggatgttcgc cgaattgggc aaaatatgcc gtctgaacac ctttccaagt caggcaaact
     5041 tcattaccat acgcgtaccc gatgccgatt tgttgtttga cacgctcaaa caaaaccgca
     5101 tcttggttaa aaaactgcat ggcgcgcacc cgcttttgga acactgcctg cgcattaccg
     5161 taggcagccc cgcacaaaac gatgccgttc tcaacatcat tcgccaactt tactgccaac
     5221 caacggattt cctatgaatt tgactaaaac acaacgccaa ctgcacaact ttctgaccct
     5281 cgcacaagaa gcaggttcgc tgtccaagct cgccaaactc tgcggctacc gtacccccgt
     5341 cgcactctac aaactcaaac aacgccttga aaagcaggca gaagacccag atgcacgcgg
     5401 catccgtccc agcctgatgg caaaactcga aaaacacacc ggcaaaccca aaggctggct
     5461 cgacagaaaa caccgcgaac gcactgtccc cgaaaccgcc gcagaaagca ccggaactgc
     5521 cgaaacccaa attgccgaaa ccgcatctgc tgccggctgc cgcagcgtta ccgtcaaccg
     5581 caatacctgc gaaacccaaa tcaccgtctc catcaacctc gacggcagcg gcaaaagcag
     5641 gctggatacc ggcgtaccct tcctcgaaca catgatcgat caaatcgccc gccacggcat
     5701 gattgacatc gacattagct gcaaaggcga cctgcacatc gacgaccacc acaccgccga
     5761 agacatcggc atcacactcg gacaagcaat ccggcaggca ctcggcgaca aaaaaggcat
     5821 ccgccgttac ggacattcct acgtcccgct cgacgaagcc ctcagccgcg tcgtcatcga
     5881 cctttccggc cgccccggac tcgtgtacaa catcgaattt acccgcgcac taatcggacg
     5941 tttcgatgtc gatttgtttg aagaattttt ccacggcatc gtcaaccaca gtatgatgac
     6001 cctgcacatc gacaacctca gcggcaaaaa cgcccaccat caggcggaaa ccgtattcaa
     6061 agccttcggg cgcgccctgc gtatggcagt cgaacacgac ccgcgcatgg caggacagac
     6121 cccctcgacc aaaggcacgc tgaccgcata aaaaaccata ccgtctgaaa cacccgcagg
     6181 cttttcagac ggtatcggaa cagataagat tacactacac tacaaacaga aaaggagtaa
     6241 acatcatgtc cgcaaacgaa tacgcacaaa tcggctggat aggcttaggg caaatgggtc
     6301 tgcctatggt aacgcggctc ttggacggcg gcatcgaagt cggcgtatac aaccgctcgc
     6361 ccgacaaaac tgcccccatc tccgccaaag gcgcaaaagt ttacggcaac accgccgaac
     6421 tcgtccgcga ctatcccgtc attttcctga tggtttccga ctatgccgcc gtgtgcgaca
     6481 tcctgaacgg agtccgcgac ggattggccg gcaaaatcat cgtcaacatg agcaccatct
     6541 ccccgaccga aaacctcgcc gtcaaagcac ttgtcgaagc cgcaggcgga cagtttgccg
     6601 aagcacccgt ttccggatcg gtcgggcccg ccaccaacgg cacgctgctg attctgttcg
     6661 gcggcagcga agccgtttta aacccgctgc aaaaaatatt ttccctcgtc ggcaaaaaaa
     6721 ccttccattt cggcgatgtc ggcaaaggtt cgggcgcgaa actcgtcttg aactcgctct
     6781 tgggcatttt cggcgaagcg tacagcgaag cgatgctgat ggcgcggcag ttcggcatcg
     6841 ataccgacac catcgtcgaa gccatcggcg gctcggcaat ggactcgccc atgttccaaa
     6901 ccaaaaaatc cctgtgggca aaccgcgaat tcccgcccgc cttcgccctc aaacacgcct
     6961 ccaaagacct caacctcgcc gtcaaagagc ttgaacaggc aggcaacacc ctgcccgccg
     7021 tcgaaaccgt tgctgccagc taccgcaaag cagtcgaagc cggctacggc gaacaggacg
     7081 tttccggcgt ttacctgaaa ctggcagaac actgattgcc ttttccaaac acaatgccgt
     7141 ctgaacatat ttcagacggc atttttatca ccccacgctt aaaatcagtc ccgattatga
//
LOCUS       DAWKQJ010000100         7149 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000100 DAWKQJ010000000
VERSION     DAWKQJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.100"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(779..1462)
                     /locus_tag="WJL20_001461"
     CDS             complement(779..1462)
                     /locus_tag="WJL20_001461"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ7683605.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1759..4062
                     /gene="parC"
                     /locus_tag="WJL20_001462"
     CDS             1759..4062
                     /gene="parC"
                     /locus_tag="WJL20_001462"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ7683606.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIVKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTI
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4082..5599
                     /locus_tag="WJL20_001463"
     CDS             4082..5599
                     /locus_tag="WJL20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ7683607.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5651..6118
                     /locus_tag="WJL20_001464"
     CDS             5651..6118
                     /locus_tag="WJL20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7683608.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRFMRYRMERLNIG"
     gene            6222..6974
                     /locus_tag="WJL20_001465"
     CDS             6222..6974
                     /locus_tag="WJL20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7683609.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1720 a         2203 c         1783 g         1443 t
ORIGIN      
        1 atagttgcgt agggtgggct tcagcccacc aactcccacc aactcccacc atcttcaccc
       61 taaaaccccg tcatctccgc aaaagcaatt gcgtagggtg ggcttcagcc caccgttttt
      121 cttcattttc ggttgttgat tggcaggctg aagcccaccc tgcggttaca aaaatcccgt
      181 cattcccgcg caggcgggaa tccagacctt tcggggcgga aacttatcgg ataaaacgat
      241 ttcccctgca tcctggattc ccactttcat gggaatgacg ggatataggt ttccctacgg
      301 acgtgttcag attcccgcct tcgcgggaat gacggcggag aagtttctgt ctttccgata
      361 acttaccgta acttaaaatc ccgtcattac cgcaaaagca gttgctacgg ttacaaaaat
      421 cccgtcatcc ccacgaaagc gaaaatcctg cctgtcggat ttcggttttt tcgggcgttt
      481 cgggaactta taaatcgtca ttcccgcgca ggcgggaatc cagaacgcgg gacggcggca
      541 atattcaaag gttgtctgaa aattcagagg ttctagattc ccgcctgcgc gggaatgacg
      601 gcggagcggt ttctgctttt ccgataaatg accgcaacct aaaacccatc cttcccacga
      661 aaacagaaaa acaaaaacct aaaatcccgt cgttctcgcg aaagcagttg ctacgtttac
      721 cgtcaaaaat gccgtctgaa cgctcaagcc ttcagacggc attttttatt tcacaccctt
      781 acaggtagaa tttttcgatg actttcaaat tgtcgtccaa tttgtacacc aacggctggc
      841 cagtcgggat ttccaagccc atgatgtctt tgtcggaaat gccctcgatg tgttttgcca
      901 gcgcgcgcag ggagttgccg tgcgccgcca ccaagacgcg tttgccgctc aaaatcgcgg
      961 gggcgatttg gtcttcccaa aacggtaata cgcgctccag cgttactttc aggttttcgc
     1021 cgtcgggtac gacatcggca ggcagatggg catagcggcg gtctttgtgt gcggaaaact
     1081 catcgtcttt gtccaaaagc ggcggcaggg tgtcgtagct gcgccgccag atgcggactt
     1141 gctcgtcgcc gtattgttcg gcggtttgtt ttttgtccaa accttgcagt tggccgtagt
     1201 ggcgttcgtt cagccgccat gttttgattt gcggtacgaa cagttggtcg gattcttcca
     1261 aaacgatgtt gcaggtctta atcgcgcggg tcaggacgga tgtgaaggcg atgtcgaact
     1321 catagccgtt ttctttcagt tttttaccgg cggcggcagc ctcggcaagc ccctgctcgc
     1381 tcagcttcac gtcgcgccag cctgtaaaca ggtttttcgc gttccattcg ctttgtccgt
     1441 ggcggataaa taccagttcc atatcgtctc caatgtgtga aagtgggaaa gccttattta
     1501 taacatattt tcacatttcc cgtatttgat tcagattcag acacgcgccc actatggttt
     1561 gccgttttga tttacaataa tgccctttgc tttacattcc gcatacacaa tgtttcaaca
     1621 cacaggacga cacataaagc accgccctat gtgttgccct gatttggaag gggttgcccc
     1681 ctcccaaata aagtctgatt ctaccgcccc gaaggacaga tgtccaagtg gcgggggttc
     1741 aaccgaaaag gaaatacgat aaatatgcaa ccgcacgctc cacataccga ttccaatacg
     1801 ctgatgctcg gccgatacgc cgaacgcgcc tatctcgaat acgccatgag cgtggtcaaa
     1861 ggccgcgcgc tgcctgaagt ttcagacggc caaaagcccg ttcagcggcg cattttgttt
     1921 gccatgcgcg atatgggttt gacggcgggg gcgaagccgg tgaaatcggc gcgcgtggtc
     1981 ggcgagattt tgggtaaata ccacccgcac ggcgacagtt ccgcctatga ggcgatggtg
     2041 cggatggcgc aggattttac cttgcgctat cccttaatcg acggcatcgg caacttcggt
     2101 tcgcgcgacg gcgacggtgc ggcggcgatg cgttacaccg aagctcggct gacgccgatt
     2161 gcggaattgc tgttgtccga aatcaatcag ggaacggtgg atttcgtgcc gaactacgac
     2221 ggcgcgtttg acgagccgct gcacctgccc gcccgcctgc ctatggtgtt gctcaacggc
     2281 gcgtcaggca tcgcggtggg catggcgacc gagattccgt cgcacaattt gaacgaagtg
     2341 acgcaggcag cgattgcgct gttgaagaag ccgacgctgg aaaccgccga cctgatgcaa
     2401 tatattcctg cgcccgattt tgccggcggc ggtcaaatca tcacgccggc ggacgaattg
     2461 cgccggattt acgaaaccgg caaaggcagc gtgcgcgtgc gtgcgcgtta tgaaatcgag
     2521 aagctggcgc gcggacagtg gcgcgtcatc gtaaccgagc tgccgccgaa cgccaattcc
     2581 gccaaaatcc ttgccgaaat cgaagaacaa accaacccga aaccgaaagc gggtaagaaa
     2641 cagctcaacc aagaccagct caacaccaaa aagctgatgc tggatttaat cgaccgcgtg
     2701 cgcgacgagt ccgacggcga acatcccgtg cgactggtgt tcgaaccgaa atccagccgc
     2761 atcgataccg ataccttcat caacacgctg atggcgcaaa cttcgctgga aggcaatgtg
     2821 tccatgaact tggtgatgat ggggctggac aaccgccccg cgcagaaaaa cctgaaaacg
     2881 attttgcagg aatggctgga tttccgcacc gtaaccgtaa cacgccgtct gaaattccgt
     2941 ttgaaccaag tggaaaaacg gctgcacatc ctcgaaggcc gtctgaaagt ctttctgcac
     3001 atcgacgaag tgattaaagt catccgcgaa tcagatgacc cgaaaaccga tttgatggcg
     3061 gcgttcgggc tgaccgaaat ccaagccgaa gacattttgg aaatccgcct gcgtcagttg
     3121 gcgcgtttgg aaggtttcaa actcgaaaaa gaattgaacg aattgcgcga agaacaaggc
     3181 cgtctgaaca tccttttggg cgacgaaaac gaaaaacgca agctgattgt caaagagatg
     3241 caggcggaca tgaaacagtt cggcgacgcg cgccgcacgc tggtggaaga agccggacgc
     3301 gccgtgctga cacaaaccac cgccgacgaa cccatcacac tgattctgtc ggaaaaaggc
     3361 tggatacgca gccgcgccgg acacaatctc gatttgagcc aaaccgcgtt caaagaaggc
     3421 gaccgcctca aacaaaccct cgaaggcaga acgattttac ccgtcgtcat tctcgattca
     3481 tcgggcagaa cctacaccct tgatgccgcc gaaatccccg gcgggcgcgg cgacggcgtg
     3541 ccggtttcat ccttgatcga gctgcaaaac ggcgcgaaac ccgttgcgat gttgacagga
     3601 ttgccggaac aacattattt attatcaagc agcggcggct acggcttcat cgccaaactg
     3661 ggcgatatgg tcgggcgcgt gaaagcgggc aaagtggtga tgaccgcaga cagcggcgaa
     3721 accgttttgc cgccggttgc cgtctatgcc tcctcgttca tcaaccccga ctgcaaaatc
     3781 attgccgcca ccaatcaaaa ccgcgccctc gccttcccca tcggcgaatt gaaaattatg
     3841 gcgaaaggca aagggctgca aatcatcgga ttaaacgccg gcgaatcgat gacgcatacc
     3901 gccgtttctt ccgagccgga aatcctgatt gaaagcgaag gcaggcgcgg cgcggcgcac
     3961 aaagaccgca tccccatctc cctgcttgag gcaaaacgcg gcaaaaaagg cagactattg
     4021 cccatatcgg gcagcctgaa acagctttct tcccctaaat aaacccggtt ccgcacatat
     4081 tatggtgatt tccaaccccc gcgaacttga aaaactcaaa gaccggattc ccaatctgat
     4141 caacatcatc cgcgtcgcca tcgtttttcc gctgatgatt atgcacatcc tcgggctgga
     4201 aaccggcagc cgtgcgaacc tgcacgcttc gtggacggcg tgggcgtttt atgtttggct
     4261 cgccattgcc tgctggctga ttttcttttc catcatccat ccgcattggc aatggcagtc
     4321 gctgaaaatg ccgcgtttca gcgcggtggc ggacatcacg atgatcggcg tgctgaccta
     4381 cctgttcggc ggcatcgatt ccggcttcgg catcctgatt ctgcccttcg tcgtctgctc
     4441 ctgcctgctc agctacgggc gttaccccct gctctatgcc agctacgccg ccatcctgct
     4501 gatattcaac gccattgccg acggcgatat cggcaaatat ccgcacatat cggatgcccg
     4561 aaccgcctcg gcaaccttca tcctcgtcgc cgcctcctat ctttccgcca tcttcacctc
     4621 actgtcggtc agatacatcg acagcgccgg ccaactggcc cgcgacaacc acctcgccta
     4681 ccggcgcatc aaaggcttga gccaaaccgt actcgaacgc gttcaagaag ccgtcgtcgt
     4741 catcaatacc gaagggctgg cggtgctgtt caaccggaag gcgaaagacc ttttccccgc
     4801 actcgaaatc ggacggcgcg ccgacctgtc cgattctgcc gccgaactgt gggaccaagc
     4861 ctctccgcac actttcgaat acgtcctcgg cacactcggc ctgaccgccc gcatccgcgc
     4921 cgtgccgatg aacaaagagc cggacaagct gcttatcctc tacatccgcc cgcaaagcga
     4981 aattcaggca gaagccctgt ccgtcaaact tgccgcgctc ggacaactga ccgccaacct
     5041 tgcccacgaa atccgcaacc cgatgtccgc catccgccac gccaacgacc tgctgcgcga
     5101 aaatatggaa gcgggggcgg cagatccgtt caacgccaaa ttgtgcaaaa tcatcgacgg
     5161 caacgtctgc cgcatcgaca aaatgctcga agacatttcc tcactcaaca agcgcaacaa
     5221 aaccgaacgc gaaaccatcg gcctgatacc gttttgggaa gaattcaaac aagagttcct
     5281 gctcggccat cccgatgccg ccggctgcat ccgcttggat atgcagggca atcacccgac
     5341 cgcctatttc gatcccgccc acctgcggca gattatgtgg aatctcgcca acaacgcgtg
     5401 gcggcacagc cgcaaacagc ccggctcgat ttccgtcacc atccgccccg cgcaaaaaaa
     5461 caccgtctgc atcctctttg ccgaccgccc gaagtgcagg aacacctgtt cgaacccttt
     5521 tacaccacgg cggaaaacgg caccggcctc gggctgtatg tcgcccgcga actggcgcac
     5581 gccaatttcg gcgatttgac ctacctaccg gaagccaaat gtttcgaact cacattaccg
     5641 gaaaaaacca atgactgaac tgcaacaccc cgtcctcgtc gtcgatgacg aaaccgacat
     5701 tctcgacctg atggaaatga ccctgatgaa aatgggcttg cgcgtccata ccgcgtcagg
     5761 cgttgccgaa gccaaaaaca agctcgacag ccaacgctat tcgctcgtcc tgaccgatat
     5821 gcgtatgccg gacggctcgg ggctggaagt cgtccaacac atcaacagcc gcctgctcga
     5881 tacgccggtt gccgtcatca ccgccttcgg caacgccgat caggcacagg aagcgttgcg
     5941 ttgcggcgcg ttcgaccccg ataccatgca gatacaggac tatctcgacc aaatcgaacg
     6001 cgacattatc gaacaaaccc tcaaacaaac cgaaggcaac cgcacgcagg ccgccaaacg
     6061 cttgggcatc agcttccgtt tcatgcgcta ccgtatggaa cgcctcaaca tcggctgacg
     6121 acaaaacggc atccgcacca tctccgccca cccgaaaaaa tgccgtctga aacggcacgg
     6181 gaaagcgggt tcgccccacg cccgaacgga cacaaaacac catgaccgac atccttattg
     6241 acaacaccgc caccgaaacc gtccgcaccc tgatacgggc attccccctt gtgcccgttt
     6301 cccaaccgcc cgaacaaggc agttacctcc ttgccgaaca cgataccgtc agcctcaggc
     6361 ttgtcgggga aaaaagcagc gtcatcgtcg attttgcctc cggcgcggca caataccggc
     6421 gcacaaaagg cgggggcgaa ctcatcgcca aagccgtcaa ccacaccgcg caccccaccg
     6481 tttgggacgc aaccgcagga ttggggcgcg acagcttcgt cctcgcctcg ctcgggctgg
     6541 ccgttaccgc cttcgagcaa catcccgccg tcgcctgcct gctttcagac ggcatccgcc
     6601 gcgccctcct caatcccgaa acgcaagaca ccgccgcgcg catcaccctg catttcggca
     6661 acgccgcagt acaaatgccc gcacttgttc aaacacaagg caaacccgac atcgtctatc
     6721 tcgaccccat gtatcccgaa cgccgcaaaa gtgccgccgt caaaaaagaa atgacctact
     6781 tccaccggct cgtcggtgaa gcgcaagatg aggccgccct cctccatacc gcacgccaaa
     6841 cggcaaaaaa acgcgtcgtc gtcaaacgcc cccgcctcgg cgaacacctt gccggacaag
     6901 cccctgccta ccaatacacc ggcaaaagca cccgcttcga cgtttacctg ccctacggga
     6961 cggacaaggg ataacgccca taaaacaaga caccgaaaaa tttgccgttc ttatgcaaac
     7021 gagaaaccgg tttttgcgtt tcgactgttt tggataaatc atcacacctt aaagtttgtc
     7081 attcccacgg aagtgggaat ccagaaatga aaagcaacag gaatttatcg taaatgaccg
     7141 aaaccgaac
//
LOCUS       DAWKQJ010000101         7090 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000101 DAWKQJ010000000
VERSION     DAWKQJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.101"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(70..429)
                     /gene="rnpB"
                     /locus_tag="WJL20_001466"
     ncRNA           complement(70..429)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WJL20_001466"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(508..1611)
                     /gene="prfB"
                     /locus_tag="WJL20_001467"
     CDS             complement(508..1611)
                     /gene="prfB"
                     /locus_tag="WJL20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ7683610.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1714..2607)
                     /locus_tag="WJL20_001468"
     CDS             complement(1714..2607)
                     /locus_tag="WJL20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ7683611.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIVKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2949..3398
                     /locus_tag="WJL20_001469"
     CDS             2949..3398
                     /locus_tag="WJL20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7683612.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3472..3780
                     /locus_tag="WJL20_001470"
     CDS             3472..3780
                     /locus_tag="WJL20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683613.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3896..4738
                     /locus_tag="WJL20_001471"
     CDS             3896..4738
                     /locus_tag="WJL20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ7683614.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNTVNHGLLSPEAFLENVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4728..5474
                     /locus_tag="WJL20_001472"
     CDS             4728..5474
                     /locus_tag="WJL20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ7683615.1"
                     /translation="MQPETSAQYQHRFAQVIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5496..6083
                     /locus_tag="WJL20_001473"
     CDS             5496..6083
                     /locus_tag="WJL20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ7683616.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6089..6268
                     /locus_tag="WJL20_001474"
     CDS             6089..6268
                     /locus_tag="WJL20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ7683617.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6500..6940
                     /locus_tag="WJL20_001475"
     CDS             6500..6940
                     /locus_tag="WJL20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683618.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1808 a         1885 c         1616 g         1781 t
ORIGIN      
        1 aacacaacct aaataaaaat gccgtctgaa ccatatttca ggtttcagac ggcatttgcg
       61 tatcggatgc acaccggacg ggcggtaagc cgggttctgt ctcggacagt cattcctcta
      121 ggcataccgt taccggtatg ctcaagcaac ctacccgaac gctcggcggg cagcgtcatt
      181 gcgttctgtt tggtcttgct ccgaatgggg tttggcctgc cgcatattgt taccaaatgc
      241 gcggtgcgcc cttaccgcac cttttcaccc ttgcctgtgc tgccaaagca gccatcggcg
      301 gttttgcttt ctgttccact ttccgtcgcg ttaccgcgcc cggccgttaa ccggcattct
      361 accctgcgga gcccggactt tcctccccgt atgccttacg cgatacgcgg cgactgtctg
      421 ctcgtcccgt gtgcggcgcg gattataaca cgaaacgcaa aaatgccgtc tgaaacggta
      481 caggtttcag acggcatacg gttcaaacta cacgccctgt ttcaggctgg cttcgataaa
      541 gccgtccaaa tcgccatcca atacggcttt ggtgttgccg acttcgtagc ctgtacgcaa
      601 gtctttgata cgtgaggaat ccaaaacata cgaacggatt tggctgcccc aacctacatc
      661 ggatttacct tcttccaacg cctgtttctc ttcattgcgt ttgcgcattt ccaattcata
      721 cagtttggac ttcaacattt ccatcgcggc cgctttgttg gcgtgttgcg aacggtcgtt
      781 ttgacattgc accacaatcc ccgtcggctc gtgggtaata cgcacggcgg agtcggtttt
      841 gttgatgtgc tgaccgcccg cgcccgatgc gcgataggtg tcgatacgca aatcggcggg
      901 gttgatttcg atttcgatgg aatcatcgat ttcagggtaa acgaacacgg aggcaaacga
      961 agtatggcgt ttgttgttcg agtcaaacgg tgaatagcgc accaagcggt gaacgccagt
     1021 ttcggtacgc agcaaaccat aggcgtattc gccttccact cggatagtcg cgcggttgat
     1081 gcccgcgatt tcgccgtcgt cttcttcgag gatttcgatt ttgaagcctt tgcgctcggc
     1141 gtagcggctg tacatgcgga acagcatacc cgcccagtct tccgcttccg taccgcccgc
     1201 gcctgcggtg atgtcgataa agcagttgtt cgggtcggcg ggctggttga acatccgttt
     1261 gaactccaaa tccgccatct gtttttccag ccccgccaca tcttcctgca cggcggcaaa
     1321 accttcttcg tcgttttctt cgacggtcat ttcaatcagc atgcggttgt cttcgatgcc
     1381 cgaagcgatg ttgtcgagcg tcaacacgat gccttcgagg attttgcgct ctttgccgat
     1441 ttcttgggcg cgtttcgggt cgttccaaag ttcggggtct tcggaaagac cgataacttc
     1501 ttccaatcgg tctttcttac cctgataatc catataaact cggatgtctt cgctgcgctt
     1561 ttccaaatca ttcagggtat tgttgagctg gttgattact tcggcttcca tgattctttt
     1621 gttctttcaa aattttaggg gcgtattgta cgggattcgg gtattttttt ctatggataa
     1681 agccttctag aaacacgttc ggacggcata gcgtcaataa cggtatgccg ccagtttgcg
     1741 tttgatttca ggcaatgcgg cacgtgctgc ctcctcaccc aactggatgg cacgtttttt
     1801 ctgatcgaat ccgccgactg cacccaaatc caaaacctgc ggtttgataa ccacatccgc
     1861 ctgccccaac tcattttgca acgcggaaac gctcattacg ttcagcgtct gatcgagata
     1921 agagaagaag ccttggctga tgtttttgcc cggacgggcg gaaatatcga cggcaatcac
     1981 gaaattcgcc ccctgccgcc gggcggcact gacgggcacg ggctgcgaca gaccgccgtc
     2041 aacatatgta tgccttccga tgataacggg ttggaacaca ttgggaatgg cggcggaagc
     2101 gcgcacagcc tgcccggcat tcccttgatt gaaagcgacg gccttgccgg tttcaaaatc
     2161 agtagcaaca gcggcaaatt tgatgggaaa ctgctgaatc tgcctgccgc cgacttttcg
     2221 gttgatgtaa ttttgcagct tttcgccttt gataaaacca ctggtggaca aggttaaatc
     2281 gactaaatcg gttttgccta aaatttcggc ttccaattcg aggcggtcgg gcgacatacc
     2341 cgatgcaaaa aggctgccga caatcgaacc tgccgatgtg ccggtaacca ccttcacagg
     2401 aataccgttt tctttcaaaa ccttaacaat acctacatgg gcaaatcctt tagatgcgcc
     2461 gccaccgagt gccaaaccga ccactgcggc gggtttggcg gtttgcaccg gcttgcgggc
     2521 agcattattt cccgccgtgc cgcaggcggc aagcaacgcg gcggcggcga ttgccaaaag
     2581 cggtctgatt tttgaaaacg ttaccatatt ttccattcct ttatatatcg caccccgtca
     2641 aaaagaggga ttgcttttct taacaacccc ctttgacagc caagcaaatg ggggctttgt
     2701 taagtcatca tcaaaattaa tatttctttt tttcctttac ggaaattata tttgaaggca
     2761 tactatccaa ggcgggaatt atctcacaac accgccgtta tccaaatatc ccgccttttt
     2821 cctttccttt ccatcaaaat actttctttt tatattcatt aacttgttaa atcattggct
     2881 gccgggtgtc agtttttccg acaaaatccg tctaatgggg tatcaacaga accaaaacag
     2941 gaacacttat gaaaatcgga acaacttggc agacggcatc cgctatgctg gttttgcgtc
     3001 tgtttgccgc atatgaattt ttggaatcgg gtttgcaaaa atggaacggg gagaattggt
     3061 tttccgaaat caacgatcag tttccattcc cgttcaactt gctgccggac gcgttaaact
     3121 ggaatctcgc catgtatgcg gagcttttac tgcccgtact gttgcttttg ggtttggcaa
     3181 cgcgtctgtc ggcattaggg ctgatggtcg ttaccgccgt cgcttgggct gcggttcacg
     3241 ccggttcggg ttacaatgtc tgcgacaacg gttataaaat ggctttaatt tatatcgtgg
     3301 tattaatccc gctgcttttc cagggtgcgg gcggatggtc gctggatacg ctgctgaaaa
     3361 aacggttttg cccccaatgc cgtctgaagc aagattgatt cagtcgtgga atctgacttt
     3421 aaacattcca accttatctc gttaacttga tattctgaaa aggaaataga aatgaacaaa
     3481 aacattgctg ccgcactcgc cggtgcttta tccctgtctt tggccgccgg tgcagttgct
     3541 gccaacaaac cggcaagcaa cgcaacaggc gttcataaat ccgcccatgg ctcttgcggc
     3601 gcgtccaaat ctgccgaagg ttcgtgcggc gcggctggtt ctaaagcagg cgaaggcaaa
     3661 tgcggcgagg gcaaatgcgg tgcgaccgta aaaaaaaccc acaaacacac caaagcatct
     3721 aaagccaagg ccaaatctgc cgaaggcaaa tgcggcgaag gcaaatgcgg ttctaaataa
     3781 tcccacccct tcaaaccaag ccgcgttttt cagtaaaatg cggctttttt aacggcaaac
     3841 aaagattttt taacaagcac atcattcttt tgtgccatcc gaaccgggta aaaatatgat
     3901 tcaacacgca ggcttgggct accgccgcga cttggcggaa gactttctct cgctttccga
     3961 aaacagcccg atatgcttta tcgaagccgc accggaaaac tggctgaaaa tgggcggctg
     4021 ggcgcgcaaa cagtttgacc gtgtggcgga acggctgccg ctggcgttgc acggattgtc
     4081 tatgtcgctg ggcgggcaag caccgctgga tactgatttg atagacggca tcaaagaaat
     4141 gatgcgccgt tacgattgca cgtttttctc cgaccatttg agctactgcc acgacggcgg
     4201 tcatctttac gatttgttgc cgctgccctt caccgaggaa atggtgcatc atacggcgcg
     4261 gcgtatccgt gaagtgcaag accgtttggg ctgccgcatc gccgtggaaa acacatccta
     4321 ctatctgcat tccccgcttg ccgagatgaa cgaggtcgag ttcctcaacg ccgtcgcacg
     4381 tgaggccgat tgcggcattc atctggatgt gaacaatatc tacgtcaaca ccgtcaatca
     4441 cggcctgttg tcgccggagg cttttttgga aaatgtggat gcagatcgcg tgtgctatat
     4501 ccatattgcc ggacatgacg tggaaacgcc ggaattgttg attgatacac atggcgcggc
     4561 agttttgccg actgtttggg acttgctcga acttgcctat gccaagctgc cgacgattcc
     4621 gcccaccctg ttggaacgcg attttaattt cccgcctttt gccgaactcg aagccgaagt
     4681 cgccaaaatc gccgattatc aaacgcgtgc cggaaaggaa taccgccgtg cagcctgaaa
     4741 cctccgccca ataccagcac cgtttcgccc aagtcatacg cgggggcgaa gccgcagacg
     4801 gtctgccgca agaccgactg aacgtctata tccgcctgat acgcaacaat atccacagct
     4861 ttatcgaccg ctgctatacc gaaacgcggc aatactttga cagcgaagaa tggagccgtc
     4921 tgaaagaagg tttcgtccgc gacgcgcacg cccaaacgcc ctattttcaa gaaatccccg
     4981 gcgagttcct acaatattgc caaagcctgc cgctttcaga cggcattttg gcactgatgg
     5041 attttgaata tacccaattg ctggcagaag ttgctcaaat tccggatatt cccgacattc
     5101 attattcaaa tgacagcaaa tacacacctt cccctgcggc ctttatccgg caatatcgat
     5161 atgatgttac cgatgatttg catgaagcgg aaacagcctt gttaatatgg cgaaacgccg
     5221 aagatgatgt gatgtaccaa acattggacg gcttcgatat gatgctgcta gaaataatgg
     5281 ggtcctccgc gctttcgttt gacaccctcg cccaaaccct tgtcgaattt atgcctgagg
     5341 acgataattg gaaaaatatt ttgcttggga aatggtcagg ctggactgaa caaaggatta
     5401 tcatcccctc cttgtccgcc atatccgaaa atatggaagg caattccccg ggccaaaacc
     5461 atctatccgc ataaaattac cttgttcccg atactatgcc gctacccgac ctgaccgatg
     5521 ccgaattaat agagtcgcgt aaactgcttc tgcattttgc gcggcttcag ttgcccgacc
     5581 accctgattt ggctgaagat ttagtgcagg aaacattgct gtccgcatac agcgcaggcg
     5641 acagttttca aggcagggca cttgtcaaca gctggctttt tgccatattg aaaaacaaaa
     5701 ttattgacgc attacgtcaa atcggaaggc aaaggaaagt ctttaccaca ctggatgacg
     5761 agctactgga tgaagcattt gaaagccatt tttcccaaaa cgggcattgg acgccggaag
     5821 ggcagccgca acattggaac actccggaaa aatcattaaa caacaacgaa ttccaaaaaa
     5881 ttctgcaaag ctgcctatac aacctgcctg aaaacaccgc acgggtattt accctgaagg
     5941 aaatactcgg tttttcatcc gacgaaatac aacaaatgtg cggtatcagc acgtccaact
     6001 accacaccat tatgcaccgc gcccgagaat cattgcgcca atgcctgcaa atcaaatggt
     6061 tcaaccaaga aaacccgaag taaacgttat gaaaaaatgc cgcgatatcg ccctgcttct
     6121 ttccaaacat caggaccggg aaaccacccc gggcgagaag atttccatat acacacacct
     6181 gctgttctgt ccgtattgcc gtgaatataa aagacaactt caaaccatca aaagatcact
     6241 ggcaaaaaca accagaactt caaaataaat gccgtctgaa aaggcttcag acggcataag
     6301 ctgacggaaa aaaatcaaac cgatttactg ttatcggcag ttcatccata atacacgctt
     6361 caaaagcagc atattcctcc atacggaata tataaatacg caaaacacga aggctgcatc
     6421 aatttgcttt atttgcctta tttcacagac ggcgctaccc ctcccgccca acccgttctt
     6481 tctgaatgag cagatttcaa tgattaagga aaccctaatg cgcccaatct tcctatcttt
     6541 cgttttactc cctattttga taaccgcctg cagcacaccg gacaagtctg cccgatggga
     6601 aaatatcggc acaatctcaa acggcaatat tcatacatat atcaataaag acagcgtgag
     6661 aaaaaacgga aatctgatga ttttccaaga taaaaaagtt gttaccaatc taaaacaaga
     6721 acgttttgcc aacacccccg catacaagac tgccattgcc gagtgggaaa tccactgcaa
     6781 caacaaaaca taccgcttaa gttcgctaca gttgtttgat acaaaaaaca cggaaatttc
     6841 cacacaaaac tacacagcct cttccctccg cccgatgagc atcctgtccg ggacattaac
     6901 cgaaaaacaa tatgaaaccg tatgcggaaa aaaactctga ttgcaactta tacacaaact
     6961 tacccacaaa ccttatcata aaaatgccgt ctgaaatact gaaatatcag catttcagac
     7021 ggcattttgc cattccctga aaattatcca caaagttatc cacattattt tttaaaaccg
     7081 gcttccatcc
//
LOCUS       DAWKQJ010000102         7006 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000102 DAWKQJ010000000
VERSION     DAWKQJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.102"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(73..1494)
                     /locus_tag="WJL20_001476"
     CDS             complement(73..1494)
                     /locus_tag="WJL20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ7683619.1"
                     /translation="MRHLPLFSLILFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSIAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="WJL20_001477"
     CDS             complement(1854..2213)
                     /locus_tag="WJL20_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ7683620.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="WJL20_001478"
     CDS             complement(2210..2815)
                     /locus_tag="WJL20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ7683621.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDKELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="WJL20_001479"
     CDS             complement(2862..3344)
                     /locus_tag="WJL20_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ7683622.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3856..4956
                     /gene="gcvT"
                     /locus_tag="WJL20_001480"
     CDS             3856..4956
                     /gene="gcvT"
                     /locus_tag="WJL20_001480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ7683623.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5119..5505
                     /gene="gcvH"
                     /locus_tag="WJL20_001481"
     CDS             5119..5505
                     /gene="gcvH"
                     /locus_tag="WJL20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ7683624.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKIKPVNPADYDVLLTAEQYAGEVD"
     gene            5657..6904
                     /locus_tag="WJL20_001482"
     CDS             5657..6904
                     /locus_tag="WJL20_001482"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ7683625.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1784 a         1683 c         1966 g         1573 t
ORIGIN      
        1 tcggaagggg ggatattgat ttgattatgc cggaatttaa aatgccgtct gaatgttcag
       61 acggcatagc gtttacagca gtttgaaaac gaaaaagata agggtatgta cgatgaagac
      121 gggtgtcagg aaggcgatcg accacatcat atagccgaag aaagtcggca tcggtacgcc
      181 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggttagtgc
      241 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg tacccgtcat
      301 caaggcttgg gcatcgccgc ccgccatatt gaaaaaaacg agataagtgg gcgcgttatc
      361 caagaatgcc gacaatatgc cgctcatcca aaaatacatc acattaatcg gatgacctgc
      421 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
      481 gctcaggacg ggaaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
      541 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
      601 ggcggtcaat gcaatcagga tgacatcgcg gacgaggttt tgcagggcgt aacggctgcc
      661 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
      721 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
      781 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
      841 atagaaggcg gtcaacagga cagcggtgct gatcaggacg ggggcgaaca tatgtttgac
      901 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
      961 ggtcagaccg ccgccgatgt ttgcaaccag gaaaatgaag aagatgacga tgtgcacgcg
     1021 gcgggtacgg ttttggttgg ctttcagcag cggacgaatc atcagcattg ctgcgccggt
     1081 cgttcccatg atagaggcaa gtgccgtacc gacggcaagc agggcggtgt tgagcttggg
     1141 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     1201 cagcaggatg aaggggatgt attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     1261 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtgat
     1321 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     1381 caaaatcagg gcaaagggca ggctccacag caggtttagg tttgcgccgt ccaaatctgc
     1441 ggcgtaaacc gatgcgggga aaagtatgag tgaaaacagg ggtaggtggc gcatcgtgtt
     1501 tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg atacaggcac cgtgccgccc
     1561 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     1621 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgct
     1681 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     1741 tctttaataa ataatctatc gaaaacgaaa aatgaattta ttttaacata tatttgcaat
     1801 gaaacaggtt tgcccccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcagaaa aatgccgatg
     2161 attcccaaca gcagggagat gccgccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagcg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtctgaat ccgtgccttc aaattggaaa tcgggatgaa
     2461 acggagcgat ttggacgatg ccttccaagc cgttttcgac aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttctttgtcc aagtcttcca
     2641 aaaaaccgtc aaggtgtttg gcttcgctga tcgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcaca ggtgcgcggg tggcatctga acaaaaaatc
     3841 cggagaaact tgagaatgat tactctgaaa accaccccat ttcatcaagc ccatcaagat
     3901 gcaggcgcga agccggtaga ttttgccggc tgggagctgc ccatccatta tggttcgcaa
     3961 atcgccgaac acgaagccgt gcgcaccgac gccggtatgt ttgacgtatc ccatatgctt
     4021 gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc gcaaattgat tgccaacgat
     4081 gtcgccaagc tcgcttttgt cggcaaagcc ctttattccg ctttgctcaa cgacaacggc
     4141 ggcgtgattg acgacttgat cgtttaccgc accaatgaag ccgaaaccca ataccgcatc
     4201 gtgtccaacg gcgcgactcg cgaaaaagat acggcacaat tccacaaagt cggacaagag
     4261 ttcggtgttt ccttcaatcc gcgctacgac ctcggcatgc tcgccgtaca aggccctaaa
     4321 gccattgaaa aactcctgac cgtcaaaccc gaatgggcag atgtcgtcca taacctcaaa
     4381 ccgttccaag gcgcggattt gggcaacgac tggtttgtcg cccgcaccgg ctacaccggc
     4441 gaagacggcg tcgaagtcat cctgcccggc accgaagccg tcgcattctt caaagccctg
     4501 caagcagcag gcgtacaacc ttgcggtctc ggcgcgcgcg acaccctgcg catggaagcc
     4561 ggcatgaacc tctacggcaa cgatatggac gacgatacca gcccgctcga agcaggcatg
     4621 ggttggaccg ttgatttgaa agacgaaagc cgcgattttg tcggcaaagc tgccttgttg
     4681 gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggtt tgctgctcgg caaaggcggc
     4741 atcctgcgcg cgcatatgga agtgttgacc gacaaaggca aaggcgaaac caccagcggc
     4801 gtattctctc caagtctgaa acaatccatc gccatcgccc gcgtaccgaa agattttgac
     4861 ggcgataccg ccaaagtgct gattcgcggc aaggaagcgg acgtgcgcgt actgaagctg
     4921 ccgttcgtcc gcaacggtca aaaacagttt gattaatatc ggttcagacg gcattttcat
     4981 ttcatatgcc gtctgaaagc aggttttaat tgtcgtccga tacggacgtt tgtagaaagc
     5041 attgaacaag gcatctgtgg atattgattc atgcagatgc cgtctgaaaa taacccccta
     5101 tcaatggagt atcaaaccat gagcagcaac atcccggcag aactgaaata cgttgccagc
     5161 cacgaatggc tgcgccttga agaagacggt atcatcaccg tcggtattac ccaccacgcg
     5221 caagagctgt tgggcgacat cgtgtttgtc gagctgcctg aagtcggcgc aaaccttgcc
     5281 gccgaagaac aagccggtgt ggttgagtct gtaaaagccg cgtccgacgt gtacgcaccg
     5341 attgcaggtg aagtcgttgc cgtcaacgaa gatttgccaa gcgctccgga aactgccaac
     5401 agcgatcctt acggtgcagg ctggttcttc aaaatcaaac ctgtcaatcc tgccgattac
     5461 gacgttctgc tgactgccga acaatacgcg ggcgaagtgg attaaaccgc ccggctgccc
     5521 gatggcaacc gccggacaaa cggaaactgc accttcagac ggcatttttg cggtcggagg
     5581 tgcagttttt tgtccgtgtt ttaaggaagc agttaggcta taataacggt ctatattcat
     5641 ctttaccgat tttttcatgc aacttaccgc tgtcggactc aatcatcaaa ccgcaccttt
     5701 aagcatacgg gaaaagctgg cgtttgccgc ggcctgcctg cccgaagccg tccgcaatct
     5761 tgcccgaagc aatgcggcaa cggaggcggt aatcctttct acctgcaacc gtaccgagct
     5821 ttactgtgta ggtgattcgg aagaaatcat ccgttggctc gcagactatc acagccttcc
     5881 catagaagaa atcagcccct acctttatac tttggggatg caggagactg tgcgccatgc
     5941 tttccgcgtc gcctgcggct tggattcgat ggtgttgggc gagccgcaga ttttaggaca
     6001 gattaaggat gcggtcaggg ttgctcaaga gcaggaaagt atgggtaaga aactcaatgc
     6061 cctgttccaa aaaacctttt ctgttgctaa agaggtccgt accgatactg ccgtcggcga
     6121 aaactcggtt tccatggctt ccgcttccgt caagttggca gagcagattt tccccgacat
     6181 cggcgatttg aatgtcttgt ttatcggtgc gggtgagatg attgagctgg ttgccactta
     6241 ttttgccgcc aaaagtcccc ggctgatgac ggttgccaac cggacgctgg cgcgtgcaca
     6301 ggagttgtgc gacaagctcg gtgtcaacgc cgaaccgtgc ctgctgtccg atctgcctgc
     6361 cattttgcat gagtacgacg tggtggtttc ttcaacggca agccagttgc ccattgtcgg
     6421 caaaggtatg gtggagcgcg cattgaaaca aaggcagagt atgccgttgt ttatgcttga
     6481 cttggccgtg ccgcgagaca ttgaggcgga agtcggagat ttgaacgatg cctatcttta
     6541 tacggtggac gatatggtca atatcgtcca aagcggcaag gaggcaaggc agaaggccgc
     6601 cgccgccgcc gaaacgctgg tgtccgagaa ggttgccgaa tttgtcaggc agcagcaggg
     6661 caggcagagt gtcccgttaa tcagggcatt gagggatgag ggagagaaag cgcgcaaaca
     6721 ggtcttggaa aatgcgatga aacagcttgc caaaggcgca acggcagaag aggttttgga
     6781 aaggctgtcg atccaactga ccaacaagct gctgcattcg ccgacccaaa ccttgaataa
     6841 ggcgggggaa gaagataaag atttggttca cgccgtcgcg cagatttatc atttggacaa
     6901 ataacggtgc gccggaaaag gatgccgtct gaaggggttt cagacggcat tttttgtgca
     6961 ggccggcaac atcgtgaaat cccacattat atcgatgtaa tcacaa
//
LOCUS       DAWKQJ010000103         6885 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000103 DAWKQJ010000000
VERSION     DAWKQJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.103"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WJL20_001483"
     CDS             complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WJL20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ7683626.1"
                     /translation="MSDNVPTIAAVATASGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WJL20_001484"
     CDS             1555..1710
                     /locus_tag="WJL20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683627.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPTKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WJL20_001485"
     CDS             1707..2138
                     /locus_tag="WJL20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ7683628.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6573
                     /gene="app"
                     /locus_tag="WJL20_001486"
     CDS             2272..6573
                     /gene="app"
                     /locus_tag="WJL20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ7683629.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYQKDTKHYYHEDYHN
                     PRLNKFVTDAAPVEMTINMNGNTYGNITDYPDRVRIGSGRQYWHKKNGNPQQIFGSYS
                     YRIAGNTHNQVGGSDGHVSLNGDVKKPNNYGPLPTGVSFGDSGSPMFIYDAKKQKWLI
                     NGVLREGNPYQGTHNGYPIVRKAYLTKIQDGDTGASIYDMVGGASYLWEAKELGKSTI
                     SQENNPKFSQMSDIANPSLPKQNKYTPSLDHRKHITFGEYVEGGVGKLTLKSNIDQGA
                     GGLYFEGDFIVSPTQNETWKGAGISISDESTIIWKVNGVANDRLSKIGKGTLHVQAKG
                     ENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNPDKLYFGFR
                     GGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNNNSLDSKKE
                     IAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGKLFFSGRPT
                     PHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQGGQAVISRNVAKVEGDW
                     HLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKVIASLTKTDISGNVSLA
                     DHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGNAQATFNQATLNGNTSA
                     SGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADKAVFHFENSRFTGQLSG
                     SKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSVSDTPRRRSR
                     RSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKLAESSEGTYTLA
                     VNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQLIRKDGEFRLHN
                     PVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKTESVAEPARQAGGENVG
                     IMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARRDLPQPQPQPQPQPQPQ
                     RDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFR
                     AYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGR
                     FDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFGGFGIEPYIGATRYFVQ
                     KADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTDAASGKVRTR
                     VNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1870 a         1966 c         1655 g         1394 t
ORIGIN      
        1 accaaacgga tcggattccc gtttttatgg gataacggaa tgttcagccg gacaccgcca
       61 cgatgctttc gatccgttta ttttccgata caaaacctcg aaaaaatcac gccgagcagg
      121 tcgtccgccg taaactcgcc cgtgatttcg ccgcatgcga cctgcgccaa gcgcaagtgt
      181 tcggcaaaca gctcgatttg atggttgccg cacaatgccg ccagcgacaa ttcttcctgc
      241 gctgctttga gtgcgttgac gtgccgcgtc cgcgccaaaa acagcccttc gccctcgccc
      301 tgccatccgg cctcgcgcaa caacgtccgt ttcagcgcgt ccaagccgtc gccggttttc
      361 gccgacagcg cgatgacggt ttctgcgccc gtaccgaacc cgcctgccgc gtgtgcgtgc
      421 aaatcggatt tgctgtggat ttcgatgcgt ttcaactccg gcggcaacgc gtccaaaatc
      481 gcccgtgtct tttcattcaa accctcgcgc ggatcgacca acaccagcgc gacatcggct
      541 tcggatacgg ctttgcggct gcgttcgatg ccgatacgct cgaccacgtc gtccgtctcg
      601 cgcaaacctg ccgtatcgac aatatgcacc ggcacgccgt caatcaggat acgttccctg
      661 accgcgtcgc gcgtcgttcc ggcaatatcg gtaacaatcg ccacttcgtc gccagccaac
      721 gcgttcagca ggctggactt gcccacattc ggcgcgccga ccaatacgac attcagacct
      781 tcgcgcaaaa tcgcgccctg ctgcgcgttg gcaagcacat catccacggc gcggcgcaag
      841 ccgtccagtt tgccgcgtgc gtctgccgct tcgagaaaat caatgtcttc ctcgggaaaa
      901 tccagcgtcg cttcgaccag catccgtaag gtaatcaagt cttcgaccag accgtgtatc
      961 cgccgcgaaa aatcgccctt gagcgagcgc aacgccagac gcgccgccga acggctggat
     1021 gcgtcaatca aatccgccac gccttccgcc tgtgccaagt ccagtttgtc gtttaaaaac
     1081 gcacgtttgg taaactcgcc cggttcggca aggcgcgcgc ccaactccaa acagcggttc
     1141 agcagcatat ccatcaccac cggcccgccg tgtccctgaa gctcgatgac atcttcaccc
     1201 gtaaaacttg ccggtgcggc aaaaaacagc aaaagcccgc tgtcgattgc ctgaccgtcc
     1261 gcgtccgtaa aatcagcata ggttgccacg cgcggcttgg gcgttttacc gcacaaagcc
     1321 tctgccatcg gcagcaggtt tttccccgat atgcgtatca cgcccacgcc gccgcgccct
     1381 gatgcggtag cgactgccgc aatcgttgga acgttatccg acataaaacc cccgaaaatt
     1441 caaaacagcc gcgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 cataaaaccg caaaaacgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgccct cctgctcggt ataggctcgc tgttgggcct gttccatccc
     1621 acaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgtaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg cgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggtcg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ctgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg agggaagcaa tcccgatcag caccgctttt cttaccaaat tgtgaaaaga
     2701 aataattatc aaaaggatac aaagcattac tatcacgagg attatcataa tcctagatta
     2761 aataaatttg ttactgatgc cgctccagta gaaatgacca ttaatatgaa tggtaatact
     2821 tatggaaata taactgacta tcccgatcgt gtgcgtattg gatcagggcg acaatattgg
     2881 cataaaaaaa atggtaaccc tcaacaaatc tttggtagtt atagttaccg aattgctggt
     2941 aatacccata atcaagtggg aggaagtgat ggacacgtta gtttaaatgg ggacgtaaaa
     3001 aaaccaaaca actatggccc tcttcctacg ggtgtttcat ttggagatag tggttctcca
     3061 atgtttattt atgatgcaaa aaaacaaaaa tggctaatca atggtgtatt aagagaaggt
     3121 aatccatacc aaggaacaca caatggctat ccaattgtac gtaaagccta tttaacaaaa
     3181 atccaagatg gagatactgg agcgagtatt tatgatatgg tgggtggggc ttcttatctg
     3241 tgggaagcta aagaattagg aaaaagcact attagccaag aaaataatcc aaaattttct
     3301 caaatgtcag atattgctaa tcctagcttg cctaagcaaa acaaatatac cccatcgtta
     3361 gatcacagaa aacacattac ttttggagaa tatgttgaag gcggagttgg taaacttact
     3421 ttgaaatcaa atattgatca aggtgcaggc ggtttgtatt ttgagggaga ttttatagtt
     3481 tcgcctaccc aaaatgaaac atggaaaggg gcgggtatat ctatcagtga tgaaagtacc
     3541 ataatttgga aagtcaatgg cgtggcaaac gaccgcctgt ccaaaatcgg caaaggcacg
     3601 ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga cggtaaagtt
     3661 attttagatc aacaagcaga tgaaaataat aaaaaacaag cctttagtga aatcggcttg
     3721 gtcagcggca gggggacggt gcaactgaat gccgataatc agttcaaccc cgacaaactc
     3781 tatttcggct ttcgcggcgg acgtttggat ttgaacgggc attcgctttc gttccaccgt
     3841 attcaaaata ccgatgaagg ggcgatgatt gtcaaccaca atcaagacaa agaatccacc
     3901 gttaccatta caggcaataa agatattgct acaaccggca ataacaacag cttggatagc
     3961 aaaaaagaaa ttgcctacaa cggttggttt ggcgagaaag atacgaccaa aacgaacggg
     4021 cggctcaacc ttgtttacca gcccgccgca gaagaccgca ccctgctgct ttccggcgga
     4081 acaaatttaa acggtaacat cacgcaaaca aacggcaaac tgtttttcag cggcagaccg
     4141 acaccgcacg cctacaatca tttaggaagc gggtggtcaa aaatggaagg tatcccacaa
     4201 ggagaaatcg tgtgggacaa cgactggatc aaccgcacgt ttaaagcgga aaatttccat
     4261 attcagggcg ggcaggcggt gatttcccgc aatgttgcca aagtggaagg cgattggcat
     4321 ttgagcaatc acgcccaagc agtttttggt gtcgcaccgc atcaaagcca tacaatctgt
     4381 acacgttcgg actggacggg tctgacaaat tgtgtcgaaa aaaccattac cgacgataaa
     4441 gtgattgctt cattgactaa gaccgacatc agcggcaatg tcagccttgc cgatcacgct
     4501 catttaaatc tcacagggct tgccacactc aacggcaatc ttagtgcaaa tggcgataca
     4561 cgttatacag tcagccacaa cgccacccaa aacggcaacc ttagcctcgt gggcaatgcc
     4621 caagcaacat ttaatcaagc cacattaaac ggcaacacat cggcttcggg caatgcttca
     4681 tttaatctaa gcaacaacgc cgcacaaaac ggcagtctga cgctttccga caacgctaag
     4741 gcaaacgtaa gccattccgc actcaacggc aatgtctccc tagccgataa ggcagtattc
     4801 cattttgaaa acagccgctt taccggacaa ctcagcggca gcaaggatac agcattacac
     4861 ttaaaagaca gcgaatggac gctgccgtca ggcacggaat taggcaattt aaaccttgac
     4921 aacgccacca ttacactcaa ttccgcctat cgccacgatg ctgcaggcgc gcaaaccggc
     4981 agtgtgtcag acacgccgcg ccgccgttcg cgccgttccc tattatccgt tacaccgcca
     5041 acttcggtag aatcccgttt caacacgctg acggtaaacg gcaaattgaa cggtcaagga
     5101 acattccgct ttatgtcgga actcttcggc taccgaagcg acaaattgaa gctggcggaa
     5161 agttccgaag gcacttacac cttggcggtc aacaataccg gcaacgaacc cgtaagcctc
     5221 gatcaattga cggtagtgga agggaaagac aacaaaccgc tgtccgaaaa ccttaatttc
     5281 accctgcaaa acgaacacgt cgatgccggc gcgtggcgtt accaactcat ccgcaaagac
     5341 ggcgagttcc gcctgcataa tccggtcaaa gaacaagagc tttccgacaa actcggcaag
     5401 gcagaagcca aaaaacaggc ggaaaaagac aacgcgcaaa gccttgacgc gctgattgcg
     5461 gccgggcgcg atgccgccga aaagacagaa agcgttgccg aaccggcccg gcaggcaggc
     5521 ggggaaaatg tcggcattat gcaggcggag gaagagaaaa aacgggtgca ggcggataaa
     5581 gacagcgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac cgccttcccc
     5641 cgcgcccgcc gcgcccgccg ggatttgccg caaccgcagc cccaaccgca acctcaaccc
     5701 caaccgcagc gcgacctgat cagccgttat gccaatagcg gtttgagtga attttccgcc
     5761 acgctcaaca gcgttttcgc cgtacaggac gaattggacc gcgtgtttgc cgaagaccgc
     5821 cgcaacgccg tttggacaag cggcatccgg gacaccaaac actaccgttc gcaagatttc
     5881 cgcgcctacc gccaacaaac cgacctgcgc caaatcggta tgcagaaaaa cctcggcagc
     5941 gggcgcgtcg gcatcctgtt ttcgcacaac cggaccgaaa acaccttcga cgacggcatc
     6001 ggcaactcgg cacggcttgc ccacggcgcc gttttcgggc aatacggcat cggcaggttc
     6061 gacatcggca tcagcacggg cgcgggtttt agcagcggca gtctttcaga cggcatcgga
     6121 ggcaaaatcc gccgccgcgt gctgcattac ggcattcagg cacgataccg cgccggtttc
     6181 ggcggattcg gcatcgaacc gtacatcggc gcaacgcgct atttcgtcca aaaagcggat
     6241 taccgctacg aaaacgtcaa tatcgccacc cccggtcttg cgttcaaccg ctaccgcgcg
     6301 ggcattaagg cagattattc attcaaaccg gcgcaacaca tttccatcac gccttatttg
     6361 agcctgtcct ataccgatgc cgcttcgggc aaagtccgaa cacgcgtcaa taccgccgta
     6421 ttggctcagg atttcggcaa aacccgcagt gcggaatggg gcgtaaacgc cgaaatcaaa
     6481 ggtttcacgc tgtccctcca cgctgccgcc gccaaaggcc cgcaactgga agcgcaacac
     6541 agcgcgggca tcaaattagg ctaccgctgg taaccgccgg atatgccgaa aggggtctga
     6601 cgatgccacc gtgcgctgtc aaaccctttt tctgccgccg ccttgtgcct gctccattgt
     6661 ctgataaccg tcaaattgat ttttagccca tgtttggtgg gtcggggaaa tctatatctt
     6721 cgtccgtgcc gaaatagtct gagacctttg caaaattcct ttccctcccg acagccgaaa
     6781 cccaaacaca ggttttcgtc tattttcgcc ccaaatacct cctaattcta cccaaatacc
     6841 cccttaatcc tccacggaca cccgataatc aggcatccgg gctgc
//
LOCUS       DAWKQJ010000104         6854 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000104 DAWKQJ010000000
VERSION     DAWKQJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.104"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="WJL20_001487"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="WJL20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ7683630.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WJL20_001488"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WJL20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ7683631.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="WJL20_001489"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="WJL20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ7683632.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WJL20_001490"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WJL20_001490"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ7683633.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="WJL20_001491"
     tRNA            complement(3332..3408)
                     /locus_tag="WJL20_001491"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3532..4701
                     /gene="tgt"
                     /locus_tag="WJL20_001492"
     CDS             3532..4701
                     /gene="tgt"
                     /locus_tag="WJL20_001492"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF001032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ7683634.1"
                     /translation="MPSFKIYFDPVAQLTFNLMLKFTLHKKDGLARRGTLELNHGKIE
                     TPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWD
                     KPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQ
                     LDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESL
                     RGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVA
                     HGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYL
                     HHLHRAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            4711..5793
                     /locus_tag="WJL20_001493"
     CDS             4711..5793
                     /locus_tag="WJL20_001493"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ7683635.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRSKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            6089..6490
                     /locus_tag="WJL20_001494"
     CDS             6089..6490
                     /locus_tag="WJL20_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7683636.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6553..6843)
                     /locus_tag="WJL20_001495"
     CDS             complement(6553..6843)
                     /locus_tag="WJL20_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683637.1"
                     /translation="MSKYVTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEQVEGGRIDIGGFMLTAKEVDGDGNIGSMGLKVLR"
BASE COUNT         1731 a         1855 c         1594 g         1674 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacgg gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcgttt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc agtttcaaac acttgaaccc acaataccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttaatttc
     1081 cttgatttgt acctgctttt gatttttctt ggcttcgtcg cgcttcttgg cttgttggta
     1141 tttgtattta ccgtaatcca tcagtttgca tacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcacggcag
     1621 taatccgctt gattttcggt gatattcata atcaccattt gaaccggcgc cagccataac
     1681 gggaaagagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggccgcgcg cggtcgttgt tttcggttac atattctgca
     1801 tccaagcgtt ccggcaagac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctt tgacgtgata ttcgatttta ggcccgtaaa atgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ccgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccaa actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     2101 acgatttgat cttcggtaca gaaaatatgc gcatcatcct gcacgaaccc acgaacccgc
     2161 atcagaccat gcaatgcacc gctcggctcg ttgcggtggc aggaaccgaa ttccgccaaa
     2221 cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattgtct
     2341 ttgtagtttt cccagtgtcc ggatttttcc caaaaggttt tatccatgat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gtgcttcgtc ttgcaaatgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggt ccgcggcaca tatcgacata ttcctgatga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaattt
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     3181 ctcgccaaac cgacaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     3301 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     3481 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     3541 tttaaaatat atttcgaccc tgtcgcccaa ctgacattca atcttatgct taaatttacc
     3601 ttacacaaaa aagacggtct cgcccgacgc ggcacgctgg agttgaacca cggcaaaatt
     3661 gaaacgccgg tatttatgcc tgtcggtact tacggttcgg ttaaggcgat gaacccgcaa
     3721 aacctgcacg atatcaaggc acaaattatt ttgggcaaca cttatcattt gtggctgcgt
     3781 ccgggtttgg aagttatcgg gcaattcggc ggcctgcacg gttttatcgg ctgggacaaa
     3841 ccgattctga ccgactcggg cggttttcag gttttttctt tgtcggatat gcgcaagctg
     3901 acggaagaag gctgtacgtt caaaagcccg attaacggcg acaagctgtt tttatcgcct
     3961 gaaatttcca tgaaaatcca aacggttttg aactcggata tcgcgatgca gttggacgaa
     4021 tgcacgccgg gcgaggcgac gcgcgaacag gcgcgaaaat cgctgcaaat gagcctgcgc
     4081 tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa acccgaacgc gctgttcggc
     4141 atcgtgcaag gcgcgatgta tgaggatttg cgcgaagaat cgctgcgcgg tttggaacag
     4201 tttgattttc ccggccttgc cgtcggcggc ttgtccgtcg gcgaacccaa gcccgaaatg
     4261 taccgtatgc tgcgcgccgt cggtccgata ctgcctgaac acaaaccgca ttacctgatg
     4321 ggcgtaggca cgccggaaga cctcgtgtac ggcgtggcgc acggcatcga tatgttcgac
     4381 tgcgtcatgc ccacccgcaa tgcgcgtaac ggctggctgt tcacccgttt cggcgatttg
     4441 aaaatcaaaa acgccaaaca caagctcgac aagcgtccga tagacgaaag ctgcacctgc
     4501 tacgcctgcc aaaatttcag ccgcgcctac ctgcaccatc tgcaccgcgc cggcgaaatc
     4561 ttgggcgcac agctcaacac catccacaac ctgcatttct accaagtcat tatggcggaa
     4621 atgcgcgatg ccatagaaca aggcaaattt gccgactggc aggcgcgatt ccacgaaaac
     4681 cgcgcacgcg gcacagactg actcccggca atgccgtctg aagccgcttc agacggcatt
     4741 ttgccgcttt accctacccg cactattttt cagaaaccga tcatgcttcc attctttcca
     4801 gaaccctccc tttcctatac gcaacaaaac cgtaccgccg ttttgctgtt gaacctcggc
     4861 acgcccgacg cgccgaccgc gcaggcggta cgaccttatc tgaagtcctt tctgaccgac
     4921 cggcgcgtcg tcgaactgcc caaatggctg tggtatccca ttctgcatgg tctggtactg
     4981 acgttgcgct ccaaaaaaag cgcgcacgcc tatgaaaaaa tctggtttaa ggaaggttcg
     5041 cccttagaag tttacaccgc acgccaagct gccgcgcttg ccgaacgtat gcccgatctc
     5101 atcgtccgcc acgccatgac ctacggcaat ccttcggtgg cagacgtatt gtcggaactg
     5161 aaggcgcaag gcgcgggcag actgctggtc atcccgatgt acccgcaata tgccgcttcc
     5221 agcagcggag cggcagtaga taaggtgtgc gaacaattac tgctgcaacg gaaccaaatg
     5281 agcgtccgca ccgtttcgcg cttttatgac gataccggct acattgatgc gatgaaaaac
     5341 cacatcctcc gatattgggc ggaacacgga cgtgggaaaa aactgatgtt gagttttcac
     5401 ggcgtaccgc agaagcacca cgacctcggc gacccctatc ccgacgagtg ccgccacacc
     5461 gccaaactgc ttgccgaagc actggaactg accgaagacc aatatgtcgt atcgttccaa
     5521 agccaattcg gcagggcaaa atgggttaca ccgagcacgc aggatttgtt cggcaaactg
     5581 cccaaacaag gcgtaaccga actggacgta ttctgcccgg gctttttggc agactgtttg
     5641 gaaaccatgg aagaaatcgc cctgatgggg cgtgaacagt tttacgaagc aggcggcaaa
     5701 agctaccgct acatcccctg cctcaacgac aaccccgact ggatagacgc actcgtcgca
     5761 cttgccgaag aaaaccttgg cggctggcgt tgaaactgtt tgacccatgt ctaaaatgcc
     5821 gtctgaacgc cgtgttcaga cggcattttc ccgctacttt agcgtatgat ggcatctatg
     5881 ctggacaaca catcaagtgc aaccttatag tggattaaat ttaaaccagt acggcgttgc
     5941 ctcgccttgc cgtactattt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt
     6001 taatccacta taccaccgat tcggcaaaaa aacctttcac ccgctacgca aaatcaacaa
     6061 caaggtttca aacattaagg aaacaccaat gaacacaacc cgactgccga ccgccctcgt
     6121 cttgggctgc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga caaaagggca
     6181 aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag gccgcggaac
     6241 catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcagg tgctgctgca
     6301 cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct acaacggatt
     6361 catgcccgca accgccatca gcgatgcgga cattgccgcc gtcgccactt atatcatgaa
     6421 cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg caaaaggcaa
     6481 aaaaaactaa acagacaaaa tgccgtctga aaccggcaat ccggcttcag acggcattca
     6541 aatcaaacct ttctaacgca gcactttcag acccatagac ccgatattgc cgtccccgtc
     6601 aacctccttc gcggtcagca taaagccgcc gatgtcaata cggccgccct cgacctgctc
     6661 ctgactgtcg gaacgcttat cgaaaagctc ggcaaggctc aaaccctctt cgcccgcttc
     6721 cagcctcaaa ccgtaagcaa gtgccaaatc gtccgaacgt gctgccggcg aaacgacaaa
     6781 ctcgccgaag aaatcaaaat gctcacggac accgatatcc gtctcggtaa cgtatttcga
     6841 cattttatag tgaa
//
LOCUS       DAWKQJ010000105         6770 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000105 DAWKQJ010000000
VERSION     DAWKQJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.105"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WJL20_001496"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WJL20_001496"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ7683638.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1292..2479)
                     /locus_tag="WJL20_001497"
     CDS             complement(1292..2479)
                     /locus_tag="WJL20_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ7683639.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2476..3486)
                     /gene="dcm"
                     /locus_tag="WJL20_001498"
     CDS             complement(2476..3486)
                     /gene="dcm"
                     /locus_tag="WJL20_001498"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7683640.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KK"
     gene            complement(3544..5823)
                     /gene="nrdA"
                     /locus_tag="WJL20_001499"
     CDS             complement(3544..5823)
                     /gene="nrdA"
                     /locus_tag="WJL20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ7683641.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6351..6662
                     /locus_tag="WJL20_001500"
     CDS             6351..6662
                     /locus_tag="WJL20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ7683642.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSESAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1802 a         1507 c         1441 g         2020 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttttcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgtta tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tctttaaaca
      361 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
      421 cctgcaactc agcggcaatt tctgccattt cagaatcatc gacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacgttga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacaca gtaatgggtt ttccgccgac attgtgcacc ccttcgccca
      721 acagattgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtggat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccaac tcgggaatcg aaaccaaagg cagcaaagca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcgacattc acaggctgac caaaaaacat cggctcattc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac ataatctatt
     1261 ctctatatta attaaaaatg atctgtaagt tttaactaac ctgctgagac tttatatatt
     1321 taataaaatt ttctaaagtt aaaacatggg ttttcccaaa agttccaaat gtttttacgg
     1381 attctttagg gacaactaag gttaaattct catcactcat ttcctgaatt tgagtatcag
     1441 atataccttc ttgtagagta aataaatgct tatgaggaat tcgatctgct tcatttaaaa
     1501 cttggcgcca gcgatcctta caagtagtct ttgaacctag cattgtcagc ttttcagttt
     1561 gaaatatgaa attccctgat tcatccatcg catggtactc ttcacttcca ggaaataaaa
     1621 aatctggttt ctttttccct tctgtcttcg cttgcgtctc aaattttaag gaaaattcgt
     1681 caaatattct tgataaatgt aactctaaag attttcctgc tcttgctttt ctgcgatttg
     1741 taaaagaatg tgcaaaatta atgaagctat ccaaatcagt aatttttgat ttaataattt
     1801 caaattcacg tttttcaaaa atggaaaata attcatattc ctttgaaacc cattgtttta
     1861 acacattact aacgttagtt ttatttaatg caatattttc ccttgccaaa actgccattt
     1921 cttcagtttt ggggaagtta ggaaatagtg aaaataattt tgttaggtta tcctctactt
     1981 cttgtttttt aggtaaatat aaacttcctg gtaaaatgtt agtttctgcg ataaatattt
     2041 caatatcttc atctgaagat aaaacaaaag catggaataa taaatcttta taacaagctc
     2101 tgcacaatac caataaggaa ccactatatt tatcacttaa aaattcaaaa ttcttgccaa
     2161 aacccgtaat tctcgcctca tttctcgtgc cttggccgta atatttaaaa ttgctattag
     2221 tgacgcttcc atcttgccaa ttaattttga ttgaaatagt tttatttgtt cccttttggc
     2281 aaactacatc aaagaggtcg tctgaaaaat gtttttgaat ataaaatcct gattggtgac
     2341 taccagtagt accaacatca ttgggacgaa tataacgaca gtatactgca attgatttat
     2401 ttgctgtctg tattgctgaa gctactaagt catcggattt atttactact tgtattgtct
     2461 gagctgccaa gtcattcatt tttttaactc atttataatt tgtttagcaa tagcttgaac
     2521 caacggtaca gcaattgaat tgccaaactg cttgtatgca gctgtcttgg atactgcatc
     2581 aataacaaaa tctttaggaa atcccattaa acgcgagcac tccctaggtg tcagcttcct
     2641 aggatttttt cctttctgag ggatgagtat ttcggaacca tctttgtaat atcgtgcaga
     2701 tagagttcgt gatattccat ctaaatcaac taatccaaaa ccaaatccat taccctttgc
     2761 cttatgtttt ttagcgtaat tttgaaggta aagccataag ttatcagaaa gagtaaaaga
     2821 attatctaca tcatcttcca aaatttgctt taattttggt tgtgattctg gtggggaagg
     2881 aaaattgaaa tttatttcct tattaaaata ttgtctatca aaacctacaa taaaaatacg
     2941 ctccctattt tgaggaacat aatattttgc attcataact tgataaaata tctgatagtc
     3001 aagctcttct aaagtccctt taattacttt aaatgtattt cctttgtcat gcgaaacaag
     3061 gtttttcaca ttctctaaaa gaaaaatttt aggtcgatgt tttccaataa tttcagcaac
     3121 atcaaaaaat agagttccct gcgccttatc taagaagcct gtttctcgtc ctaggctttt
     3181 tttctttgaa acaccagcta tagagaatgg ctgacacggg aatcctgctg ttaatacatc
     3241 aaacttactt ggaatagctg ctttggtttc ctttaatgta atatctccat aaggaatatc
     3301 attaaaattt acttggtagg tttgacgggc tttatcatcc cattcactag aaaatacaca
     3361 tcgcccacca acattctcca ttgcaatgcg aaaaccacct attcccgcaa ataaatcaat
     3421 aaaagtaaat ttctcattat ttaaggtaac tatattatct aattgatttt gtattttatt
     3481 tttcatattt tatatttcag atgatttatt aatctaaagg ccatctgaaa actccccctt
     3541 tcatcaaatc ttacaagccc cgcccgcgca gccgtcatcc tgaatatcgg tctgcgtatc
     3601 gtccgcaccg tcgcgggtgt tatggtagta cagggttttg acgccgtatt tgtaggcggt
     3661 cagcaggtct ttgagcattt gtttcataga aactttgccg ccttcgaatt tgcccgggtc
     3721 gtaggcggtg ttggcggaaa tcgattgatc gacgaatttt tgcatcacgc caacaagttt
     3781 cagatagcct tcgttgccgg gaagctgcca cagggtttca taggcatttt tcagggtttc
     3841 aaactccggc acgacttgtt tcaaaatgcc gtctttcgat gctttgaccg ttaccaatcc
     3901 gcgcggcggc tcgataccgt tggtggcgtt ggcgatttgc gaactggttt cagacggcat
     3961 gagtgcggtt agagtagagt tacgcaggcc gtatttgacg atgtcggcac gcaggctttc
     4021 ccaatcgtag tgcaaaggct cgttacacac agcgtccaag tcttttttgt aggtgtcgat
     4081 gggcagtttg ccttgcgaat aaacggtttg gttaaagagc gtgcacgcac cgtattcttt
     4141 ggcaaggttt gccgatgctt tgagcaggta atactgtatg gcttcaaagg tacggtgggt
     4201 cagaccgagc gcggagccgt cgctgtagcg gacaccgttt ttcgccaaat aatacgcata
     4261 gttgatcacg ccgataccga gcgaacggcg gcccatagtg gaggtacgcg cggcttctac
     4321 cggatatccc tgataatcta aaagtgcatc gagcgcgcgc acggtcaaat cggcaagccc
     4381 ttccaattcg tccaagctgt ttaatgcccc caagttgaag gcagacaggg tacacagggc
     4441 gatttcgccg ttcgggtcgt tgatattgtc caaaggtttg gtcggcagcg cgatttccat
     4501 acacaagttg gactgatgaa caggcgcaac gcgcggatcg aacgggctgt gcgtattgca
     4561 gtgatcgacg ttttgaatgt agatgcgccc ggttccggca cgctcctgca tcagcgtgga
     4621 aaacaggtcg gcagccggaa tgatgcgctt gcggatatca gggtcttgct cgtatttcgt
     4681 atagagccgc tcaaattcgt cttggtcggc aaaaaacgct tcgtacaatc ccggaacctc
     4741 gttgggcgaa aacagcgtaa tgttgccgcc cttaatcagg cgggtgtaca gcaggcggtt
     4801 gatttgcacg ccgtaatcaa gctgacggat acggttgtct tccacgcccc ggttgttttt
     4861 caacaccagc aggctttcgg cttcgatatg ccacaagggg tagaacaagg ttgccgcgcc
     4921 gccgcgcacg ccaccttgcg aacaggattt gactgccgcc tgaaacattt taaagaaggg
     4981 aatgcagccg gtatgccgtg cttcgccgcc ccggatttcg ctgtccagtc cgcggatacg
     5041 tccggcattg atgccgatgc ccgcacgctg ggaaacgtat ttcacaatcg cgctggtggt
     5101 ggcgttgatg gaatccaggc tgtcgtcgca ttcgatcaat acgcagcttg agaactggcg
     5161 cgtaggcgta cgcacgccgc tcataatcgg ggtcggcagc gatactttaa atgtggaaac
     5221 ggcatcgtaa aaccgtttga cgtaatcgag gcgtgtttct ttgggatatt tgctgaaaag
     5281 acacatcgcc accagaacat ataagaactg cggtgtttca taaatctggc gggtaacgcg
     5341 gttctgtacc agatatttgc cttcgagctg tttgacagcg gcataggaaa aggacatatc
     5401 gcgttcgtgg tcgatatagg cgttcagctc gtcaaattct tcgcggctgt aatcctcaag
     5461 gatatgcctg tcgtattttg cggcatcggt aagtttttta acgtggtcgt aaaggtgcgg
     5521 cggctcgtac tcgccgtagg ctattttacg cagatggaaa atcgccaaac gcgcggcaag
     5581 gtattggtag tccggggtat cttccgaaat taaatcggca gcggctttga tgatggtttc
     5641 gtggatgtcg tcggtgcgga tgccgttgta gaactggatg tgcgatttca actcgacctg
     5701 agatacggaa acattttcca atccgtccgc cgcccaagtg acgacacggt gaatcttatc
     5761 caaatcaatg gcttctaatc ttccgtctcg tttggttact ttcaaatcag tcggtgtatt
     5821 catcgcttcc tcttccactc ttgatattca agacacggtc ttttcaaata aattaaggca
     5881 gacaatatag tggatttttg gcattttctc cagtcttgac aacggttgta tttttcagtt
     5941 tcaggcaatg cgcgataaaa cccgcctgtc gtatttttcc tataatattt gttttatctg
     6001 ataattcttt accgataaaa aacgggtaaa ttttttgcct tttgaccggc tccggctaca
     6061 aggcggtgaa ataaggattt tccgacgaaa aaggaaagct tcctgttttc tgcccctgca
     6121 aattgttaaa ttttgcaaag tatgattttg ccacgccgcc gccgacaaat tccattttct
     6181 taccgattgg aatttattat tgagattaat gtgttatttg aatctgcata tcaaacggca
     6241 agttttgtcg gctgaatgag tctgaaactg ccgacaattt gcccgttcca tctttccatc
     6301 catactggaa ggaatgacaa actgaaagga tcgtcatgtc tgtcaaaaag atgcttgcca
     6361 tactgttgtc tgcaatattg ggactggtat cgacaactgc cgctgccggt acgtcagaat
     6421 ccgcccaccg ccataccaaa catatccgca aggcaaataa gcagatgttg caccccgaat
     6481 gcaggaaata tttggaacgc cgtgccgcgt ggtaccgatc gcaaggcaac gtgcaggaat
     6541 tgcgcgaaaa caaaaaggcg cgcaaagcat tccactccct gccttatgcg gaacagaaaa
     6601 tccaatgccg ggcggcttat gaggctttcg atgatttcga cggcggcagt ttccgccgtt
     6661 aatcccatat aaaatatgcc gtctgaacac aggttcagac ggcattttcc atagatacaa
     6721 caaaccatcc ttacgccatt tcgtttgagg tgctcccgtt gccgcagcct
//
LOCUS       DAWKQJ010000106         6518 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000106 DAWKQJ010000000
VERSION     DAWKQJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.106"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            600..2048
                     /gene="gnd"
                     /locus_tag="WJL20_001501"
     CDS             600..2048
                     /gene="gnd"
                     /locus_tag="WJL20_001501"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ7683643.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPLGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVRMGGLFARQALPVGGDKKQWVGDLRQALLASKIISYA
                     QGFMLIREAGESYGWDLGYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2111..3382
                     /gene="waaA"
                     /locus_tag="WJL20_001502"
     CDS             2111..3382
                     /gene="waaA"
                     /locus_tag="WJL20_001502"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ7683644.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKLSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMRECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTAFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3423..3872
                     /locus_tag="WJL20_001503"
     CDS             3423..3872
                     /locus_tag="WJL20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ7683645.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3905..4879
                     /locus_tag="WJL20_001504"
     CDS             3905..4879
                     /locus_tag="WJL20_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7683646.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            4948..5023
                     /locus_tag="WJL20_001505"
     tRNA            4948..5023
                     /locus_tag="WJL20_001505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4981..4983,aa:Lys,seq:ttt)
     gene            5242..6495
                     /gene="murA"
                     /locus_tag="WJL20_001506"
     CDS             5242..6495
                     /gene="murA"
                     /locus_tag="WJL20_001506"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ7683647.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1492 a         1580 c         1895 g         1551 t
ORIGIN      
        1 tatccccatc cgaaagttta tttatacaca agcaatccaa agttatctga ctgaaatatt
       61 gtgagattta cccgcaaatc tcattttttc caccacttat acacaaattc ggatgcgcac
      121 tctgccgata tgcagaaata agacattcgt gccgacacct gaccatttcc acaagttcca
      181 aacacacttt cctctcaaac ctccacagta ttccggttct taaagacatc ggcagccatt
      241 cggtaaagaa aagcggaatg ccatctgcac aacaggcaaa ctgtccacaa aaactattct
      301 tcatgaaaaa caatccacag gcatgcacaa aagggaatcc gccttaccga caccctaaaa
      361 aaaagaaatc ctgatgattt ttaaaataaa aaaaagttac ccacagaaaa tttctcctat
      421 caatataatc atcgttttat tatttaaatt ttatatttta tttataagcc ttcagggaaa
      481 cggcacggga aagcgtaaag gcatcttttc aaaaaattaa aaagttaagg attgttaaac
      541 aagtgtaaat caggtagagt tgtggtcgat aaattaaatc tataacggag cttcctgcaa
      601 tgaaaggcga tattggcgta atcggtttgg cggttatggg gcaaaacctg attttgaata
      661 tgaacgattg cggatttaag gttgtcgcct acaaccgaac aatcggtaag gtggacgaat
      721 ttttaaacgg cgcggctaag ggaacggata ttgtcggcgc gtattccctg caagacttgg
      781 tcgataagct ggcaaaaccg agaaaaatca tgatgatggt tcgcgcaggt tcggtcgtcg
      841 acgactttat cgaacaactg ctgcccctgt tggacgagga agacatcatt atcgacggcg
      901 gcaatgccaa ttaccccgac acgacacggc ggacacatta ccttgccgaa aaagggattt
      961 tgtttgtcgg ggcgggcgta tccggcggcg aagaaggcgc gcggcacggc ccgtccatta
     1021 tgccgggcgg ggataagcgc gcttgggaag cggttaagcc gatttttcag gcgattgccg
     1081 ccaaaacgcc gctgggcgaa ccgtgttgcg actgggtcgg cagggacggc gcgggacatt
     1141 ttgtcaaaat ggtgcataac ggcatcgaat atggcgatat gcagttgatt tgcgaagcgt
     1201 accagttcat gaaagacggt ttgggactgt cctacgacga aatgcaccgc gtgtttgccg
     1261 agtggaacaa aaccgagctg gattcttatc tgattgaaat cacagcggca attttgggct
     1321 ataaggacga aggcggcgaa ccgctggtcg agaaaatcct cgatacggca gggcaaaaag
     1381 gcacgggcaa atggacgggc atcaatgctt tggatttggg cattccgttg acgctgattt
     1441 cagaggcggt gttcgcccgc tgcgtttcat cgttcaaaga acagcgcgtc cggatgggcg
     1501 gactgtttgc gcggcaggca ttgccagtcg gcggcgataa aaaacaatgg gtcggcgatt
     1561 tgcgccaagc cttgctcgcc tccaaaatta tttcgtatgc ccaaggcttc atgctgatcc
     1621 gcgaagcggg cgaaagctac ggctgggatt tgggctacgg caacactgcg ctgctgtggc
     1681 gcgaggggtg catcattcgc agcgcatttt taagcaatat ccgcgatgcc tatgaaaaca
     1741 atcccgattt ggtgttcttg ggtgcggatc cgtatttcaa aaatattttg gaaaactgcc
     1801 tgccggcgtg gcgcaaggtg gttgccaagg cggtcgaatg cggcattcct atgccctgca
     1861 tggcttcggc aattacgttc ttggacggct acacgaccga acgtctgcct gccaacctct
     1921 tgcaggcaca gcgcgactac ttcggcgcgc acacttacga gcgcaccgac aaaccgcgcg
     1981 gcgagttttt ccataccaac tggacgggca agggcggaga taccgcttcg acaacctacg
     2041 atatttgatt ccgttgccga aatgccgtcc gaaccgttcg gacggcattt gttttgcagg
     2101 aaatagaaag atgttccaat ggctttatga tgtattgtgg ctgcttgcgc cgatatggat
     2161 acggcgttat ttggacaaac tctccggaag tgccccggca tatcgggcgc atcgggacga
     2221 gcgtttcggc aagccgtatc cgaatcccgt taccggtgcg gtttggatac acgccgtttc
     2281 ggttggcgaa acgcgtgccg cccagccctt gatacgcgag ttgcggcagc gttttcccga
     2341 tgcgccgctg ctgatgacgc agatgacccc gacgggacgg gaaaccgcgc aagttctgtt
     2401 tcctgatgca caatgccgct atcttccgta tgacaaaaaa acgtgggtac ggcagttttt
     2461 gcgcgaacac cgaccgatgt tcggcatttt gatggaaacc gaaatctggc ccaacctgat
     2521 gagggaatgc cggcgcgcgg gtgtgccgct gtttttggcg aatgcgcggc tgtcggaaaa
     2581 atcgttgaac ggttatctga aggtccgccg cctgatccgt cctgccgccg cttcgctgac
     2641 gggttgtctg gcgcagacgg aggcggatgc ggcgcggttg gcgaaattgg gagcggcatc
     2701 cgtgcaggtg tgcggcaata ccaaatatga cctgatgccg tcggaacaga tgaaaacgct
     2761 ggcggggcag tttgaaaaac gcatcggagg ccggccggtt gccgtgtgcg gcagcacgcg
     2821 cgtttatcgg ggtgaagacg aggcggaaaa actgctggcg gcgtggcaac aatatcgcgg
     2881 cgatgccctg ctggtcgtcg tgccgcgcca tcccgagcat tttcagacgg catttgaaac
     2941 ggcaaaacgc ttcgggttta aggttcagcg gcgcagcgac ggtttgccgg tcgaacctga
     3001 tacgcaggtg tggataggcg acagtatggg cgagctgtat gcgtattacc tgtgcgccga
     3061 tgttgctttt gtcggcggca gtctggtcga ttcgggctgt cagaacatca tcgaaccgct
     3121 ttcctgcggc gttccgacga tattcggctt ttcgacctac aatttttccg aagcctgccg
     3181 gcacgccttg gcatcgggtg cggcggttca agtcgaatcg gcggatgcgt ggcgtgaagc
     3241 cgttgaaaaa accttatcgt ccgagggggg cggaatgcag atgcaggcgc gcgtggacgg
     3301 ctttatcgca caacatcgcg gagcgggcgc gagaatcgcc gaggcggtgc gggaagcggt
     3361 atgcggacat cgggggcgat agtgatacaa tccgtatccg agttttccgg ttggagtacc
     3421 gtatgagtat ttatgccgtc gcgcacatcg tccacctgta ttgcgccatt gcctttgtcg
     3481 gcggcgtgtt ttttgaagtg ctggttttgt ccgtcctgca tacgggacgg gtgtcgcgcg
     3541 aggcgcggcg cgaagtggaa aaggcaatgt cttaccgcgc cgtcagggtg atgccgtttg
     3601 tggtcggact gctgttcgcc agcggcatcg tgatggcggc aaaccgctat ctttctatat
     3661 tgggcgaacc gtttgccact tccttcggta cgatgctgac gctgaaaatc ctgttggcgt
     3721 tcagcgtgtt ggcgcacttc gccatcgccg tcgtcaaaat ggcgcgttcc acactgaccg
     3781 tcggctggtc gaaatacata cacaccgtcg tctttaccca tatgctgctg attgtctttt
     3841 tggcaaaagc gatgttttat atcagctggt aacacaacta catttcaaat caggagattg
     3901 aaccatgaca gaatatcccg gcatcgggtc gccgttgttt tacggcgttt tttttgcggc
     3961 agtgctggtc atgattgcct tggatatgtt ttcgctgaag aaaaacagca gccataaagt
     4021 cggcgtcaaa gaagccttgg catggagcgg cttgtgggtt gccgtatcct gcctgttcgc
     4081 aggctggctg tatttcaaac tcgccggcaa tcccggctac ggcgcggcag tcgcaaaaga
     4141 aaaagtattg gaattcttta ccggctacat tttggaaaaa tcgttggcag tcgacaatat
     4201 tttcgtgttc ctgatgatat tcggctactt caaagtcgca ccgcagtttc agcaccgcgt
     4261 gctgctgtac ggcgtattgg gcgcattggt attgcgcact gtcatgattt tcgtcggcgc
     4321 ggcactggtt cagcagttcg agtggattct gtatctgttc ggcgcgttcc tgctctatac
     4381 cggcatacac atgatgaagc ccgaaggcga tgaggaaggg gatttggcaa acagcaggct
     4441 gctgaatgcc gtcaagaaag tcgttccggt cggcacggaa tttcacggcg agaaattttt
     4501 tactgtcgaa aacggtaaaa aaatcgccac gccgctgttt ttggtgctgg tcatgattga
     4561 attgagcgat gtcgtttttg ccgtggacag catccccgcc gtctttgccg ttaccaccga
     4621 tccgtttatc gtgctgactt ccaatatttt cgcaattctg ggcttgcggg cgatgtattt
     4681 cctgctggcg gatgtggcgg aacgcttcgt attcctgaaa tacggcttgg cattcgtgtt
     4741 gggttttatc ggtgtgaaaa tgctgataat gcattgggtg catatcccga tttccgtttc
     4801 gctgtcggtc gtgttcggcg cattgggtgc atcggtgttg acttcattgg tttatacgaa
     4861 aaaacagtcc gataaataaa atgccgtctc aacgccgaca aaatggttga cagcatcagg
     4921 cttgatgtat agaatttcga gcttgtcggg tcgttagctc agtcggtaga gcagcggact
     4981 tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataaaa caaaaaagct
     5041 gagttttcaa aactcggctt tttttgtttt ccgacaatcc ttgttcggaa agcgcggcat
     5101 ttcgtcaaat tctttggtgc ggcttccgta tttttcccga aaatccctta taattccgcc
     5161 tctgcaacct tcatcgggtg atgctttatg cgttgtcccg agtattgaaa tattacaagt
     5221 acccaccagg aaagaacaaa cgtggacaaa ctgaaaatct ccgcaaacgg cccgctcaac
     5281 ggggaaataa cggtctcggg cgcgaaaaac gcggcattgc cgctgatgtg cgcgggtttg
     5341 ctgacatcgg gtacgttgcg cctgaaaaac gtccctatgc tggcagatgt gaaaaccacg
     5401 caaaagctgc ttcaggggat gggcgcgcgc gtcctgactg acaatatcag cgaatttgaa
     5461 atcaacggcg gtacggtaaa caatacctgc gccccttacg aattggtacg gacgatgcgg
     5521 gcctcgattt tggtgctggg gcctactttg gcacgtttcg gcgaggcgca agtcagcctg
     5581 ccgggcggct gcgccatcgg ttcgcgcccc gtcgatcagc atttgaaagg cttggaagcg
     5641 atgggtgctg agattgttat cgaacacggt tacgtcaaag ccaaaggcaa actcaaaggt
     5701 acgcgcgtgg cgatggatgt cgttaccgtc ggcggcacgg aaaacctgct gatggcggcg
     5761 acgctggcgg aaggtacgac ggttttggaa aactgcgcca ttgagcctga agtggtcgat
     5821 ttggcggaat gcctggtcaa aatgggcgcg aaaatcagcg gcatcggtac gtccacgatg
     5881 gttgtggaag gcgcgggcga gctacacggc tgcgaacaca gcgtcgtccc cgaccggatt
     5941 gaagcgggga cgtttctgtg cgcggtggcg ataaccggcg gcagggtggt tttgcggaat
     6001 gccgccccga aaacgatgga agtggtgttg gacaaactgg ttgaggcagg tgcggtgatt
     6061 gaggcgggcg atgattggat cgccatcgat atgcggcagc gtccgaaggc ggtggacatc
     6121 cgcacggtcg tccaccccgg cttccccacg gatatgcagg cgcagtttat ggcattgaat
     6181 gccgtggcgg agggaagctg ccgcgtggtg gaaacgattt ttgaaaaccg ctttatgcac
     6241 gtccccgagt tgaaccggat gggggcgaac attacaaccg agggcaatac ggcatttgtg
     6301 cagggtgtgg aacggctttc cggcgcagtc gtcaaggcga cggatttgcg tgcgtccgca
     6361 agcctcgtta tcgccggttt ggcggcgcgc ggcgaaaccg tggtcgaaca gatttaccac
     6421 ttggatcgcg gttatgaaaa tattgaaaaa aaactcggca gcgtcggcgc gaaaatcgag
     6481 cgcgtatccg gctgatcggg ttgcgatgcc gtctgaag
//
LOCUS       DAWKQJ010000107         6507 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000107 DAWKQJ010000000
VERSION     DAWKQJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.107"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..3758)
                     /gene="hrpA"
                     /locus_tag="WJL20_001507"
     CDS             complement(<1..3758)
                     /gene="hrpA"
                     /locus_tag="WJL20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ7683648.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIESGKPLSLETSTVASNIRLNNGNIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGLLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNY"
     gene            complement(3868..5610)
                     /gene="hrpB"
                     /locus_tag="WJL20_001508"
     CDS             complement(3868..5610)
                     /gene="hrpB"
                     /locus_tag="WJL20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ7683649.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            complement(5671..5910)
                     /locus_tag="WJL20_001509"
     CDS             complement(5671..5910)
                     /locus_tag="WJL20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7683650.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            complement(6194..6496)
                     /locus_tag="WJL20_001510"
     CDS             complement(6194..6496)
                     /locus_tag="WJL20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683651.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
BASE COUNT         1549 a         1496 c         1544 g         1918 t
ORIGIN      
        1 ctgtaattgc ttttacccac tttgatgccg ataaagcggc ggcttttttg gacatccaac
       61 tcgtgcttgt ggatgccttc ttctgccagc agttgcagct cttcacccgc aaccagggta
      121 actttacctg caggggcatt gaagcgggtg gtattagctt cgatgttgcc gcctgcctga
      181 agcgtgatac cgttggcggt cagctcgacg ggggctggca taatcaggtg gtcgcgggtg
      241 ctggtaaact tggtttttct gatgattttg ccgcttttac ctttggtttt taagaaggta
      301 taggcatcgt tttgtccagc ctccagtaca atatcactat gggctttgat gtctatgctg
      361 cctgagggag ctttgatttc ggatgcaccg ataataatac gtgcatcatc gagtgccgca
      421 gctgcatgaa tacttacccc cgtacgtccg gtcaaacgtg aaggcttgtt cagagcagct
      481 ttgtcgtagt gactcttgta ggtgggcttg ccaatttcat attggtcggt tatgccgtca
      541 atcagaatag cagccgcctc tgaatctgct gcctttggca atacgcctgc ggcgtgaagg
      601 ttcagttttt tggaagcggt aatatcggaa ccgctgattt cgatgccttg tgcggaaatc
      661 aagtcaatat tttgtgcaga aagcttggct tgcaggtatt ctttgccttt gggtttttta
      721 cctttaactt ccttgttgat ggcttgaata tagaaagcga gacggtcgcg ttcttcttgc
      781 agggttggaa tcagcttgct tttaggcgag ctttttttca actgcgcaat ctgctgttcc
      841 aattctttgg atttttggtt gagttcagcc gctttttgtg taggaaaata attgctgaat
      901 gagttgttta cggcttcgat attcaacttg cctttggtgg tggcgacaac caagttttta
      961 cctgctgtaa ttttagaacc tcttaaatct gtttctcctg taaccagacg gatattgcct
     1021 tttgcttcca atgaggaaac ttgagcacta ggcgcacctg catttcctcc ttttgcagac
     1081 aacagcaatt ttccgcctgt tttgatgctc aggtcggtat gcgcactgat gcggttggca
     1141 ggttcgatgg ttaatgtgcc gctacctgct tcaatattca gccgtccggc caatggtttt
     1201 aattcggcat tatcttccaa agttttggtc gaaacggtac tccaattgat gttgccgcgc
     1261 ttgactaggg cggtaccggc agtaaggttg attgcacccg ctctcagcgt ggtgttgtcg
     1321 gcaatttggg aatagcgcgc attgagtgcc aatacaccgt tagccaccag cttgttggca
     1381 gaaggcagtt tgtcgttttg ccaaatctgg ctgccggtaa tgcttagatg acggtgtgcg
     1441 taggcatcta cttggttgag cgttacccgc tcgtgttgtg cattaagatg cgtattatgg
     1501 gtagactcca gcttggtatt ttctatgctc aatgcccggt cggaatgaat gttcaatgcc
     1561 ccgcttttgg catggacgtt aaggtttttt aagtcggcat taccaccgtt gtttttgatg
     1621 ctgatgtgtt ttccgttgat tgaattgcgt tgttttccgt caccaagctg aataccgttg
     1681 ccggcaacca acgtaatatc tcctgaagat gaagtgatat tggtattgtc tgctttcaga
     1741 cggcctttac caaccgatcc tgcattgaca tcggccttgg ctgtcagggt attgtgaccg
     1801 gtaaagtcgg cattaccgtt ggccaataag gatacatgac cgtctgcaga aacagcatga
     1861 aggccgtctg aaacgatatt gccctgcaat gcggtggttt cgagagcctt ggctgcgttc
     1921 agcttggtat tgcgaagctg aatattgcct ttggctgcct gaatgtgcag attacccgag
     1981 ttggtacgca gattggtatt ggtaacattc agcaagcctg cctccacacc catgtctttt
     2041 gaggcagtga gggttttact ggtgccggta atatgggcag cgttatccga tttcaaatgg
     2101 atgctggcgg cagacaaatc tttatcaaca ttcaaattca gatctttacc tgtatgaaca
     2161 tacagattgc cgggagtatt cagattggta gttttggcag taatgttatc gtctgccagt
     2221 aaagcaagct gctttccgcc tttaatgtta ccgttgttca aacggatgtt ggaggcaacg
     2281 gtcgaggttt ctaaagaaag cggtttgccc gattcaatgt gtgcagtgtc tttagcctca
     2341 attacagcag cactgccaat actaccgtta gataatacgg ttacgttttc agcaataata
     2401 cgggtatttt cacccaattc agtatcgcct ttggtggagc tgtacacgga actgcccgct
     2461 tgaatagtag catcattgat cgtagtacga ccgccggccg acagattagc cgagcctttg
     2521 gcattgttca cattagtttt actctcaatc accaaattat gaccagcatt taataccgtg
     2581 gtagctgggc gactgccgtt attctgcacc acggctccgt tacgcaagct gatatcttct
     2641 cccgtctcaa taaccaataa gcctttgctc tcgatccgac caccattgga gataaatgtg
     2701 cctgccgctc ctttttcggt ggtttcgatg gagagataag tcggtgaagc ttcggtgccg
     2761 tcggcagtgg tggcgatgcg gccgctgttt tcaatgcggc ctgacgaagt cacaatcaat
     2821 tgcttggccg cttcgagtgt gccggcattt ttgacgccta cgcctttttc attggcaatc
     2881 agggtaatgc tgtcggcgta cataccaccc aatgcggcag tatcgagggc aacggtcggt
     2941 ttcgtacccg ctgccgtacc tgcgctgatt tcaccgctgg cgtaatctac tttctgtgcg
     3001 ccggtcgaaa ccgccaggtt tttaccctgt aatttcccct gcaaagcaac tgcacgagca
     3061 agtatctcgg tgtagtcggc tccgccttta tcattccaac ctgctgctcc tacggtcaat
     3121 gtgccttgac gcacatcaaa tcctgtcagt gcaccgtctt tgccgatttg gggcgcaccg
     3181 gtagttaaga tgccccgacc gacattttta aagccgccgc cattaacggt aatgccgttg
     3241 gggttggcaa taatcacatc ggccttttga ccacctacgg taacgatgcc attgagtttg
     3301 ctggccgcac cgcgcacctc gttcaaaatc aactgcgcac tgcctttggc cagaaacggg
     3361 ttgttgttgc gatcgttgtt cagtacagca cctttagcgt cgacatcaaa ctgggtatag
     3421 cggttgtggc tcaatccgcg tccattcgga gtttggatat tcaccaaggg ggcaccagtg
     3481 ttggttttaa ggataacgac ctgctggttt ttaggtgctg atttgtcggt ggtgatttgg
     3541 gcatgggcag gcaataccat actcagggaa accaaagagc agaccaaggt tttaagggtg
     3601 gttttgagtt tgccgcaaag gtcgcctgaa gttttcagtg aaacagaaac cgaactgcct
     3661 gcctgtttac ctttgccctg gctgttggca gtttcggcta ctgcaaccat ggtgctgtgc
     3721 tttttactaa agataatgcg atgtaaacct ttattcatgt ctattccatt ttgaagatga
     3781 acgtactgcg cgccaagtac gtaggtaaag tttgacagtc tgaggataag gaaagaccgt
     3841 ctaaatatca gtaaaaaaat tcagaggtta gaaactgtaa ttcaagttga agccgtaaac
     3901 ggtgttggtc gtctgaaagc ctttgggttt atgaagcggc ttgccggcaa acagatcata
     3961 agcaaacata ccgcctactt tatgccctcc tctgaagccg accactgcac ccatcagctg
     4021 cttgcccgat acatattgtg cactttcgcc agatacgcgg ccatagtccg caccgagata
     4081 gaactgatgg ttcggatgaa aataccaagt taaagtattc tgccagtaga aacctcgctc
     4141 tccgaaaaga ctctgctccc catcaaatcc gcgaacggtg tagcggctgc cgattgacaa
     4201 tttatcttgg gcaaccaacg gcgttttgtt ccattgagct tgaatggcgg ttgcgtagaa
     4261 aaactgctgt ttgcctaaaa taaatggggc ggctgcgtcc aaaccggcag taatgatttt
     4321 catacgagat gtacctggaa gaatatcgcc gccgttttct tccggtgcag gcatactttg
     4381 gcgcatgccg gtcccgcgtt tgtaagacaa cttgccgtca agctgccaac ggttgaggta
     4441 agcacggtgg cgcaattcgg cttcccagcc tgcagagcgg cggcgttgca cttcgatttc
     4501 ggcatcgtcg atgtatttat aggtttggcg tgtccataat ttcattccga ctgaagtttt
     4561 atgaagtctg ttacgccaaa gcatgcgctc ggcggccagg ctgctctgat attgtttgcc
     4621 gttgtaatcg taattgacgg aatagccttc ggttgcttcg tggtaacgat gtccattgtg
     4681 attaaaagaa aacagccatt tttttacggg caccgaataa tgcacgctgt aacttctgga
     4741 tccgcttgca gtttccgtac cggtggcatc agtcaagtcc gttttgtgcg ccaaaccgcg
     4801 tccatatgaa acataaaaca aatcgcttaa gcccaaaggg ttatcgaacg ataaagcgac
     4861 atttccttga tatttgccgg tcgttttgcc gcccgcatca tctataccga tactgaaccg
     4921 tatgggttta ttctgctgcc atttgatctg taaatcgctt ttgccttctt cttcggacgg
     4981 tataatctga atatctgttt taacactcga caaacgacgc aggttttcca agccctgctc
     5041 tacatcgcga agattgagaa ttttgttcct atataaggga aatttgttat tgaatgcact
     5101 aatactgccc tcggcagact tcccatcccg tttttcttca tagcggatat cccctatttc
     5161 gcctgctgat acccgtaatt tcagaattcc cgaatccata ttctgtggtt ggataatagc
     5221 ttgggaagtg aggtagccac gcacaatcag tatctgttgc gcggcttttt gtagcctgct
     5281 caaattattg gaacctaaac acatcccagt tttaaaagct gtttctttca tgagcacaga
     5341 aggaagaaaa gaaaatttgc gcgccgtctt atcatctaaa ctaatgtaat ttacccgagt
     5401 acacggtgtt tcatcttcac tcaggacata attgttcttc tccaatggtt gctcgaaacg
     5461 gacatttgca tcagttaaca attcagcatc tatgtgctgc tgacgctgca tggaacggat
     5521 aagttctgca tcgttttcat cggcagctaa ggttttaagg ggtatgacag ccaggataac
     5581 caacagacat ggagcaggaa aaaatttcat gacatcaata ttattttagc aatatttact
     5641 attttgtcat aaatttaaaa gtatttacag ttatagaatg agacctttgc aaaattcccc
     5701 aaaattccca ccaagacatt taggggattt tggggaattt tgcaaaggtc tcggacagta
     5761 ttttgaacgc agtgcgcgta aatccgtatg gaaaccatga aatcccgcca cagccgccag
     5821 acatgccaag ccgcattctg atatttctgt ttgcaggata acaggcagct ttttctttaa
     5881 gcccaaagac aggttttgca gatggggcat agatttcctt tttgaaaaat agggattagg
     5941 aagttggatg tattttagaa aggccgcctg aaaaggtttc agacgacctt ttgcgactag
     6001 ctgctatttt atttaaagct tttctctaac aaacgagcta atatttttcc tgtaataaaa
     6061 cagataaaaa acagcatcca atacgtcaga ttggaaaaat cggtcgtata gagaatcaac
     6121 atataaagaa gcagcatgat gccgagtgcg atgaattgat aatgtttggc aaacatcatg
     6181 acctcctcaa ctattaaggc aaaccgcctg aatattctcg ttcaatcgtt tcggcaattt
     6241 ccctataacg tcgataccat gaccagtcga aattttcaat ggcatggctc gcaaacgtac
     6301 caaattcagg catacctatg cggctacctg ctaaagctcc gattgtagct ccccaaccag
     6361 gcggattact gaacgtattg tttggcaatc ctaaagattt atcaggattt cccgtatcgt
     6421 taagccctgc agattcgcaa atttcgcaat aatatgaact ttgttgcgca ttacctgagc
     6481 ctccgaccca agtcatttca tgaacac
//
LOCUS       DAWKQJ010000108         6490 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000108 DAWKQJ010000000
VERSION     DAWKQJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.108"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WJL20_001511"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WJL20_001511"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ7683652.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRIGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WJL20_001512"
     CDS             complement(1297..2652)
                     /locus_tag="WJL20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ7683653.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="WJL20_001513"
     CDS             complement(2667..2939)
                     /locus_tag="WJL20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ7683654.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="WJL20_001514"
     CDS             complement(3065..3802)
                     /locus_tag="WJL20_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ7683655.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WJL20_001515"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WJL20_001515"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7683656.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="WJL20_001516"
     CDS             5127..5720
                     /locus_tag="WJL20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683657.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..6452
                     /locus_tag="WJL20_001517"
     CDS             5832..6452
                     /locus_tag="WJL20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ7683658.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVSTCFKADKSAEAVNNDTEHNTA
                     YFSHFPSKQKSTGGSRCPFLYRFPYFLSAGSTGLAGPFGAGAPTEA"
BASE COUNT         1761 a         1643 c         1493 g         1593 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cgacttaaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttgaaggaa
      241 atgcacggag gcaaactgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgacgag
      361 ggaatcaggc tgcgggagag tggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggattt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaagt tttcgcattt ttcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcatc
      961 ggtctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attggaccgg
     1321 agcgggaatt ctgccgcccc gtttgacgaa cacttcgcac gaaccttctt ttaccggcat
     1381 cacaggtgcg tagcccaaca ggccgccaaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcgatgatg ccggaaatgg tatgagcagg cgtatcgccg ggaacggcaa tcatatccaa
     1561 accaacggag cagacagcgg tcatcgcttc aagtttgtcc aacgtcagca caccggcttc
     1621 ggcagcggca atcatgccct cgtcttctga aacggggata aacgcaccgc tcaaaccgcc
     1681 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 cgccgtcgtg ccgtgtgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccaa cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcacccacg cgggtaattt tgaaagcagt
     1921 tttcttcact acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc
     1981 tttgaccacg cccggccccg atacgccgac attaatcaca gcatccgctt cgcctgagcc
     2041 gtgaaacgcg cccgccataa acggattgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcgcagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcgcg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccaacg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttgtcc aaagtttgcg ccacgctgac ataggaatcg gcatgggtag ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtaattttgt tgtaaatgtt
     2521 ttggttcaac acattgatat cgctgctgat gcagtcgtgc agatcaatgc cgatggtaat
     2581 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttca
     2701 ttttgcatac ggatatcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcctgccgag atttggggca tttcgaggta tccaccaaga taatcatggt aaaaaaatca
     2821 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcttca ctctttttag aaccatcttc ataccactct aagactactc
     3181 cattacgttt atgatgatgg atagacagtt ccgagagtaa tcgaccgctt tcatcccatg
     3241 tcagaatctt ggccggctca tcattgacca taaccatttc cgttttgata ttgccatcag
     3301 cataccattg tttccatacg ccgtttgcct tattttgttt aaactggatt tcgctttcct
     3361 tgctgccatt acggtaatag cggtaccccg taccttcact caaaccattt ttataaggca
     3421 taaccgcaga ttttttaccg ttcggatacc agttgaccca ctcaccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcggaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtacgc aataccctcc gccacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag tttcccgttc tgattaaaat
     3721 aaacagaata ggtctgtgcc gataaagaag ccgaaaaacc caacaggaca attgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tctgttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctactg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggcgg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgtcagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc cgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaac atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaacctg attggctggg
     5161 aaaaaacggg gcttgttgcc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca atgaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctgcgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 acacggtcaa ggtttggggt tacgcttcaa gcgaggaaat cgattcggac ggcgaagtca
     5941 tcgcgacggc agctatgaag gcggcgattc ccgattatat gaagtttggc gaggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgttgaa gacgacggca
     6061 taaccttttt cggtgctcat atcatcgacc ccgttgtcgt ggcgaaagtc aaaacaggcg
     6121 tttacaaagg cttttccatc ggcggcagcg ttaccgcccg cgatgatttg aacaagtccc
     6181 aaatcacggg cttgaagctg acggaaatca gccttgtcga ccgccctgcc aatcccgatg
     6241 cggtgtctac ctgctttaag gcggacaaaa gtgcggaagc ggtaaacaac gatacagaac
     6301 ataatactgc atattttagc catttccctt ccaaacaaaa aagcaccggc ggcagccgat
     6361 gccccttcct ttacaggttc ccctattttt tatccgcggg cagcaccggt ttagctgggc
     6421 cttttggtgc gggcgcgccg accgaagcct gatccttcag cttcgccagc accgcagggc
     6481 cgatgccctt
//
LOCUS       DAWKQJ010000109         6421 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000109 DAWKQJ010000000
VERSION     DAWKQJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.109"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..345)
                     /locus_tag="WJL20_001518"
     CDS             complement(<1..345)
                     /locus_tag="WJL20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683659.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTG"
     gene            complement(347..592)
                     /locus_tag="WJL20_001519"
     CDS             complement(347..592)
                     /locus_tag="WJL20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683660.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(600..1754)
                     /gene="lpxB"
                     /locus_tag="WJL20_001520"
     CDS             complement(600..1754)
                     /gene="lpxB"
                     /locus_tag="WJL20_001520"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ7683661.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLKRYPAARFLLPAATEATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(1820..2812)
                     /locus_tag="WJL20_001521"
     CDS             complement(1820..2812)
                     /locus_tag="WJL20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7683662.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVRLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            3306..6065
                     /locus_tag="WJL20_001522"
     CDS             3306..6065
                     /locus_tag="WJL20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ7683663.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPSNVGRLKLYQDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRVTAAILPEGVAPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         1601 a         2003 c         1549 g         1268 t
ORIGIN      
        1 accggtcgga agatcatacc taccgcctat cacttccagc gcacctccat aatccaagat
       61 ttcatcatcc tgctttaatt ctccaagttc caatacattt ttgatatttt tatcttggtt
      121 cttcgtggcg tatccataga taaatttcag atagctgaac tgcttgaatt taatttttac
      181 ccattctgaa taattgggca tagggatctc ttgaggaaag tccggataat ccacattctg
      241 gacgatgata tggaattcat cgtttttcaa agtatagttt aatagagtgc cctcatatat
      301 atacagaaat tgataatctt tttttggaat ggtataagtt gtcatattat tttacctttt
      361 caaaaacaat gagtttaaga ttttttgagt ccaagttggt tctactactc ttatgaaatt
      421 tcagttgttt aaactcttcc tttaacagac cattatattt taaccttaat cccactcgag
      481 ccagttctgc ttcatttgga attcctctta aatacttatt gtttgatgtg ccattaatca
      541 caatttctcc cccttttttt gtaatacgtg ctgcttcggg aaaataatcc attaaccgct
      601 caacatcccg cctcttccaa aaccgcgcgc gcggccaaat ccgccgtatc ttttttcaac
      661 agcaggtgca acgccctgaa atcctgttgc agcgcggcaa ccttatcggg gtgttcgtac
      721 cagtccgcca acgccgccgc cagtttttcc ggtacggcat catgttgaag cagctccggc
      781 acagcctcct tacccaacag gatattcggc aggccaacat gcggcacttt gattttgcgt
      841 ttcacataag cataagtcag cggcgaaatc ttgtagctga tgaccatcgg acgcttgcac
      901 agcgccactt ccaaagtcgc cgtaccgctc gttaccagca ccgcatccgc cgccctgcac
      961 accgtttcag actgtctgtc ggttaccgtc agcggtaatc cggcaaactc cgaacgctgc
     1021 aaaatttccg ccaaacgccg cttcgtcgcc tccgttgcgg caggcagcag gaagcgtgcg
     1081 gcgggatagc gtttcaacaa caataatgcc gtctgaaaaa acaccggcgc catatagtcg
     1141 atttcgctga cgcggctgcc gggcagcagg gcgaatacgg ggatgccggc atccacgccc
     1201 aaagtttgcc gcgccgtttc acggtcgtct tccaagggca taagctgcgc catcggatga
     1261 ccgacaaact ccgcacgtcc gcccgcatcg agataaagct gcggctccat cgggaacagg
     1321 cacagcacgc ggttgacctg atgcacgatt ttgcccacgc gttcccgccg ccacgcccac
     1381 accgacggac tgacataatg cagggtcgga atgcccgccc gtttgagccg ctccgccaca
     1441 cccagattaa aatcgggcgc atcgatgccg acaaacacat caggtttcaa cgacagcaaa
     1501 tcccgtacca gccccctgcg tatccgtaaa atttccggca ggcgttttac cacctcggca
     1561 aagccgcgca ccgccagtcg ctcctgatca taaaggctct cgaaaccttc cgccttcatc
     1621 agttcgccgc cgataccgac aaaacgcgcc tgcgggcaac gctttcggat ggcgcgtatc
     1681 aggtgcgccc ccaacaggtc gcccgacgct tcgccgacac tgacggcaat caaagggctt
     1741 tttttatcaa ccatattcgt tcttcttcac atactttcac gtcccgatgc cgtctgaagg
     1801 cttcagacgg catcggggtt cacattttcg tcccgttttc caacatcacc gcaaactgcg
     1861 ccaagtccgg cggcagctcc gccttcaaca ccagcggctc gcccgtgagc ggatggttca
     1921 agtgcagctc ggacgcgtgc aaaaacatcc gcttcaaacc caacttctgc aaacgacggt
     1981 tcgcctgata atcgccgtag cgttcgtcgc ccgcaatcgg acagccttga gattgcagat
     2041 gcacgcggat ttggtgcgtg cgccccgttt tcagcgttgc ccgcaccaaa gtcaggtgcg
     2101 acagcccgac accgtgcaaa atgccgtctg aaaaacggct taacacacgg aacaccgtgt
     2161 gcgccgactg cccgtcctcg ctgacgcgta ccatcttttc gccttgtgcg ccggtatatt
     2221 tgaacagggg cagcctgacg tggaaattat cgtccggcag tttgcccacc cccagcgcaa
     2281 ggtagatttt tttggggtgg tcgttacgga tggcttcgtg aagtttgacg agcgcgctgc
     2341 gtttcttcgc caccatcaac aaaccgctcg tatccttgtc caaacggtgg acgagttcga
     2401 gataacgcgc ctccggacgg gcgcggcgca actgttcgat aacgccaaaa ctcacgccgc
     2461 tgccgccatg caccgccacg ccggaaggct tatcgatcac caaaagcgca tcgtcttcgt
     2521 aaacaacgtc aaacgcacgc gccggtacgg cggcacgcct ttcagacggc atttccttct
     2581 ccgccacgcg cacaggcgga atccgcaccg tatccccctc cgcaatacgg ctgtcgggtt
     2641 tgcagcgttt tttgttcagc cgcacctcgc cggcgcggat aatgcggtgg atatggctct
     2701 tgggaacccc cttgaggatt tttatcagat agttatcaag gcgttgaccc gcctcatgtt
     2761 cggcaacccc tatcaagctg accgaatctt tgcttatttc gtgcgttttc atctataatc
     2821 cgaacatccg tttcagcaaa aagcgcgccc gccgcgcccc cctgaaacgg tttactttaa
     2881 aacggaattt tatattaaaa acgcactccg caaagcattt tccctgcgcc tgttcccagc
     2941 cggcaggcgc agaacgtaat caagtttgaa ttgattttgc cgtttcggcg cggaagtaag
     3001 acggcagccg agccccaagt cccaaacgca aaccgcccaa aacacaggca tcacgataac
     3061 aagaagaagc cggcccccat ttttccgacc gcagcgttcg gaacgcggca gacaaaccgc
     3121 cgcatcgatc tttatagcct tctgtatccg acccttccgc acacggttct ccggaacgtg
     3181 catccctgcg gattttcaac tcaaaaactg attaccggtt ttatccgaaa acatcggaaa
     3241 acccagcctt acggaatgcc gaaccggacc ggcgcgcccg aatgccgccc gtccacgagg
     3301 tgatcatgaa aagaatgtta ttcaacgcaa cgcaggccga agagctgcgc gttgccatcg
     3361 tcgacggaca aaacctgctg gatttggaca tcgaaacgct gggcaaagaa cagcgcaaag
     3421 gcaatatcta caaaggcatc attacccgca tcgagccgtc gctggaagcg tgtttcgtcg
     3481 attacggaac cgaccgccac ggctttttgc cgtttaagga agtatcgcgt tcatatttcc
     3541 gcgactacga aggcggcagg gcgcgtattc aggacgtact caaagagggc atggaagtca
     3601 tcgtccaagt tgaaaaagac gagcgcggca acaaaggcgc ggcgctgacc actttcatca
     3661 gcctggccgg acgctatttg gtattgatgc cgaacaatcc gcgcggcggc ggcgtatccc
     3721 gccgtatcga aggtgaagag cgccaagagt tgaaagccgc tatggcgcaa ctcgacattc
     3781 cgaacggcat gagcatcatc gcccgtaccg ccggcatcgg ccgcagcgcg gaagagttgg
     3841 aatgggattt gaactatctc aaacaactct ggcaggcgat tgaagaagca ggaaaagcgc
     3901 atcacgaccc ttacctgctc tttatggaaa gctcgctgct gattcgtgcg attcgcgact
     3961 atttccgtcc cgatatcggc gagattttgg tggataatca agaagtttat gaccaagttg
     4021 ccgagttcat gagctacgtc atgccgagca atgtaggccg tctgaaactc tatcaagacc
     4081 acacgccgct gttttcccgc ttccaaatcg aacaccaaat cgaaagcgcg ttttctcgca
     4141 gcgtcagcct gccttccggc ggcgcaatcg tcatcgacca caccgaagcc ctcgtctcca
     4201 tcgatgtcaa ctccgcacgc gccacacgcg gtgcggacat cgaagacacc gccttcaaaa
     4261 ccaatatgga agccgccgaa gaagtcgccc gacaaatgcg cctgcgcgac ttgggcggct
     4321 tggtcgtcat cgacttcatc gacatggaaa accccaaaca ccagcgcgat gtggaaaacg
     4381 tcctgcgcga cgcgctcaaa aaagaccgcg cccgcgtaca gatgggcaaa ctctcccgtt
     4441 tcggactttt agaattgagc cgccaacgtt tgaaaccggc tttgggcgaa agcagccacg
     4501 tcgcctgtcc gcgctgcgct ggcactggtg tcatccgcgg catcgaatcc accgccctgc
     4561 acgttttgcg catcattcaa gaagaagcga tgaaggacaa caccggcgaa gtgcgcgcac
     4621 aagtgcccgt cgatgtcgcc accttcctgc tgaacgaaaa acgcgccgag ctgtttgcga
     4681 tggaagagcg tttggatgtc aacgtcgtcc tgattccgaa catccacctc gaaaatccgc
     4741 actacgaaat caaccgcatc cgcaccgacg acgtagaaga agacggcgaa ccgagctaca
     4801 aacgcgtcgc cgagcctgaa gaagacgaat ccgccaaacc gttcggcggc gaaaaagcca
     4861 aagccgcccg tcccgaaccc gccgtcaaag gcgtgcgcca caccagcccc gccccgactg
     4921 ccgcccccga gaaaaaaacc tcctggtggg acagcttcaa agcttggctc aaacgcattt
     4981 tcggcggcag cgaaacccaa gccgcgcccg ctgccgaaac ctccgaaaaa cgcagcacgg
     5041 caaaccgcag cggcagccgc gccaacaacc gccgccaaaa cccgcgccgc agcaaacgcg
     5101 aaggcagcaa agtggaagtc cgcgaagtgg caggcaaaac tgccggacag gaagcgcgtg
     5161 ccgacaaagc cgaaacgcgc aacaacggca accgccgccg caacgaacgc ggcgaccgtg
     5221 ccgccgaacg cgccaacgaa gcggaaatcc aagaccgcaa catacagcct gccgcaaccg
     5281 ttgcagatgc cgcaccgtcc gaaaccgaag tgcaaaccgg aaaacgccgc cgcaacggca
     5341 gccgcagcga acgcggccaa accgcgccgg aaaccgccgc cgttgccgaa acaaccgttc
     5401 agacagcgga aaacacgccg tccgaaccgc ataccgcaga agacaaaggc agcaagccca
     5461 aatccgaacg caaccgccgc gaacgcgaca gccgagatgc caaagaacgc cgcgagcgca
     5521 acaatcagcg cgaccgccgt caaaacggca aaaaacgcaa tattccgtct gccgccaaaa
     5581 tcgagcagta cctgaatatt cacgacaccg ccgacaaagt ccgttccgcc gccgcgcacg
     5641 ttttcggcga aaccgacgca aacgcgccga ttaccgtcag cattgccgat ccggttgcag
     5701 aaagggatct tccgacagca tctcccgccg tttcaaacgg cgacgcaccg gtttatgatg
     5761 cggcggaaaa aatccgccgt gtcaccgccg ccatcctgcc cgaaggcgta gcaccgaaag
     5821 ccgaagcaca ggaaatgccg tctgaaaccg caacctttac ggctgcggcg gaacaggcac
     5881 gggaaaccgc acaaaccggc ggactcgtcc tgatcgaaac cgaccctgcc gcattgaagg
     5941 catgggcggc acaacccgaa gtccaaaccg gacgcggttt gcgccgttcc gaacagccca
     6001 agccgtctga agtcgcaacc gtccctgccg aagaaatgat ccaagtcgaa acccggcaag
     6061 gctgaaccga cggcggcaaa aagaggttct gttccgcaga acctcttttt tacatagatt
     6121 cggatacctg caatgccgtc tgaaacttcg ccattcccgt cattcccgca acctttcgtc
     6181 attcccatga ttcccgcaac attccgtcat tcccgtgaaa acgggaatct agaacctcaa
     6241 actttcggat aatctttgaa tattgccgtc gcccaaaggc ctggattccc gcctgcgcgg
     6301 gaatgacggt ttagaagttg cccgaaatct caaaaaaaac cgaaaccgaa caagccagat
     6361 tcccgcaaca ttccgtcatt cccgtgaaaa cgggaatcta gaacctcaaa ctttcagata
     6421 a
//
LOCUS       DAWKQJ010000110         6345 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000110 DAWKQJ010000000
VERSION     DAWKQJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.110"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(224..2104)
                     /locus_tag="WJL20_001523"
     CDS             complement(224..2104)
                     /locus_tag="WJL20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ7683664.1"
                     /translation="MNIFYEESGQFKVAAIVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGMKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            2260..2892
                     /locus_tag="WJL20_001524"
     CDS             2260..2892
                     /locus_tag="WJL20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ7683665.1"
                     /translation="MGNTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            2964..4334
                     /gene="purB"
                     /locus_tag="WJL20_001525"
     CDS             2964..4334
                     /gene="purB"
                     /locus_tag="WJL20_001525"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ7683666.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            4415..6283
                     /locus_tag="WJL20_001526"
     CDS             4415..6283
                     /locus_tag="WJL20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7683667.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
BASE COUNT         1370 a         1650 c         1735 g         1590 t
ORIGIN      
        1 ccgctttcgc gggaatgacg gcatatcagt ttctgtgcgg ataaacctag attcccatct
       61 gcgtgggaat gacgggattt tagattgcgg gtatttatcg ggaacggcgg cttggaagtt
      121 cattgaaacg gaaaaacaac ggaaacccaa aaaaccggat tcccgactgt gggaatgatg
      181 agattcaggt ttctgttttt gccggagttt gccgtatcgg gcttcagacg gcattgcctg
      241 ccgttgtacc cgcgggtgcg actgccttga tgtagttgag cgagacaaac tgcttctcgg
      301 catccaattc ggtgattttg aacaatgcct gtgatttggg cagcgcgtca aacggaatac
      361 cggtcgcgcg tgttactagc ggcagacctt cgatgcggac gaggtcttct ttcagaatcg
      421 tcgccgtcag ctcgctgatg ccttgctgtt gcaggtaaac caggctccag taggcttcca
      481 tttggcgttg gaagtcggcg taggcggtat aggcggcatc aaagtcgcgc agtgcggcga
      541 aaagctcggc atcgctgttt tgatacagcg gctcggcagt gtcgtctatc aggctgatca
      601 gctgcttttg gttgatgtag tcggcggcgc ggcgcagcgg cgaggtaaac cagccgtaat
      661 gccgcacgcc catgccgata tgcggctcgg atttggtgct catgcgtact tttccggtgg
      721 gttggacgcg gaagaggccg ggcaggtcgt tgtcatggag catttgtgcc caagtgctgt
      781 tggcaagaat catcatctcg ctgaccagcg tatcgatggg cgaaccgcgt tcgcggcgga
      841 cgacggatac gttgtcttct ttatccaatt cgatgctgta atcgtattgc ggcgcgcggt
      901 cgggttcgta tttgccgcgc gctttttgca gggcggtggc gaattgataa aaccaaatca
      961 ggtcttgatg gtgggcgaac atcatttcgc ccgcttcgtc caagcccgct tcggcgttga
     1021 aatgcggttc gatggcttgg atacgcaggt tttcagcgat gttgaccgct tcgattttgc
     1081 aggtcggcgc gccgacgttg aactcgttgt ccacatcgaa ataaatgctg atggaagggc
     1141 ggtatgtacc tgcatcaagg ctgaacgcgg caatccagtt ttcgggcaac atcgtgattt
     1201 tgccgccggg aaaataaacc gtgcttaagc gttccatgat gtttttttcc attttgtcgc
     1261 cttgcttaat ggcaagcgac ggcgcggcga tgtggatacc gacgcgtttc atgccattgc
     1321 ccaagtcggt caggctcaag gcatcatcga cttcggtggt cgactcgtcg tcaatggaaa
     1381 aggcggtaac gtcagctttg ggcaggtcgg gcatttcagg aagcggaagg tcggggaagc
     1441 ctgttccttt agggaaatat ttgatttcaa acccgtcttg caggtattgg ggaatggacg
     1501 taatgccgcc cgtttttttc gccaattcgt aggcagaggt tttcagcgcg tcggcggctt
     1561 tggtaaaggc tttgtaggtc agcgactgct tgtcgggcgc gtgcaggatg gttttcaaat
     1621 ccgccgcgat ttcagacggc atctcgccgc gtttcaaggc ttccgcccaa gcgtcgattt
     1681 gcgcgtcttg ctgttttttg cgttcgatgg cggcaagtgc ttgttttaaa gtttcttcgg
     1741 gcgcggcttt gaacacgcct ttggcttttt tgtagaaata catcggcgcg gcgtacagcg
     1801 caatcaaggt cgccgccagc tcggttttgg tcggcgcatg gccgtaatat tcttcggcaa
     1861 tcgcctcggc ggaaaattct tcttcgccgc atacttccca caataaatcg gtgtcgatgt
     1921 ccgccgcctg tgcctgcgcg ttttccaaaa acgccgccat atcgccgtca aactcggcaa
     1981 agacattgtt tgccttcact ttggtgcgtt taccgtgttg ggtatcgact tggtaggtgg
     2041 cgtcgttttt ttggacgatg gcggcgactt tgaattggcc ggactcttcg taaaaaatat
     2101 tcatttttcg gatttttctg tggaaactca agcgggcgat tttagcagat taccgaaaat
     2161 gccgtctgaa aaaaggttgg gggagggttg gcgcgtcttt gcggtgcttg cgttacgttt
     2221 ttgacggggt acaatcgcca tttttgaacg gaggacggaa tggggaatac aaaccgtgtg
     2281 ccggagcagg cacgttatga tgccgaacgc aggcaggcag acgaagcatt ggcgggcgtg
     2341 tttccggcag tcagtatctt cggcagcgcg cgcacgccgc agaatcatgc ggattatgcg
     2401 ttcgcctgcc gtctggcgcg gcggctgtcg gattcgggca ttgccgtcat ttcgggcggc
     2461 gggccgggga ttatggaggc ggcaaacaag ggcgcgtttg cagggaagtc ggtttcggtg
     2521 gggctgaaca tcgttttgcc gcacgagcag aaaccgaatc cgtatcagga catcgccttg
     2581 cggttttccc gttttgccga acgcaaggcg gtgtttttcc gctattccca agcatatgtc
     2641 gtgatgccgg gcggcttcgg gacgctggac gaattgtttg aaatcctgac cttggtgcag
     2701 acgggcaaag tgccgccgcg tccgattgtt ttggtcggaa aggcgttttg gtcgggcttg
     2761 gcggagtgga taaacgcgca gcttttggcg cgcggtctga tttccgaagg ggcagcctct
     2821 ttgttcgcca tatcggacga tgaagacgaa atcgttgcgt atctgtcgga acacgggctt
     2881 cagacggcat agcgtcctga gagtgatgta taattgcaaa caatttaaca atttttgatg
     2941 tctttcccga acaggatgcc gaaatgatca accccatcgc ctcgctttcc cctttagacg
     3001 gccgttatgc ccaatccgtt gaagcattgc gcccgatttt ttccgaatac ggcctgatga
     3061 aagcgcgcgt caaagtcgaa ttaaactggc tcaaagccct cgccgccgag ccgaagattg
     3121 ccgaagtgcc gcccttcagt gccgaaacgc ttgccgaaat cgacacggtg attgaaaact
     3181 tttcattgga agacgcggcc gccgtcaaag ccatcgaagc cacgaccaat catgatgtca
     3241 aagccattga atattggctg aaaaaacgtt ttgccgaagt gccggaagtc gccgccgtga
     3301 gtgagttcat ccacttcgcc tgcaccagcg aagacatcaa caacctgtcc cacgctttaa
     3361 tgctgcaaga agcgcgtgag gctgttttgc tgccgaagct ggccgaaatc atcgaaaaac
     3421 tgaccgctat ggcgcacgac cttgccgccg tcccgatgat gagccgcacc cacggccagc
     3481 ccgccacgcc gaccactttg ggcaaagaaa ccgccaatgt cgtgtaccgc ctgcaacgcc
     3541 agtttaaaaa cctgcaagcg caagagttcc tcggcaaaat caacggcgcg gtcggcaact
     3601 acaacgccca tatggtcgcc tatcctgatg tagattggga aacccactgc cgcaacttcg
     3661 tcgaaatcag cctcggtctg accttcaacc cctacaccat ccaaatcgag ccgcacgact
     3721 atatggcgga attcttccaa accctcagcc gcatcaacac gattctgatc gactttaacc
     3781 gcgacgtttg gggttatatt tcattgggtt acttcaaaca aaaagtcaaa gcgggcgaag
     3841 tcggctcatc gaccatgccg cacaaagtca accccatcga ctttgaaaac tccgagggca
     3901 acctcggtat ggccaatgcc gtattgggct ttttgtccga aaaactgccg atttcccgct
     3961 ggcagcgcga cctgaccgac agcaccgtat tgcgcaatat gggcgtgggc gtgggctatg
     4021 ccgtgttggg tttcgccgcc cacctgcgcg gtctgaacaa gctcgaaccc aaccctgccg
     4081 cgcttgccgc tgatttggat gccacttggg aactgctcgc cgagccgatt caaaccgtga
     4141 tgcgccgtta cggtgtcgcc aatccttacg aaaaactgaa agacctgacg cgcggcaaag
     4201 gcggcatcac gcccgaagtg ctgaaaggct ttatcggatt gctggaaatc cccgccgaag
     4261 ccaaagccaa attgcttgag ctgacccccg cgctgtatgt gggcaaggct gaagcgttgg
     4321 cgaaacggat ttgagcgttt actgaaaccg atgccgtctg aacgcgcgtt cagacggcat
     4381 ttttaagata acgggacata cgggggcgat atttatgcaa gctgtccgat acagaccgga
     4441 aattgacgga ttgcgggccg tcgccgtgct atccgtcatg attttccacc tgaataaccg
     4501 ctggctgccc ggaggattcc tgggggtgga cattttcttt gtcatctcag gattcctcat
     4561 taccggcatc attctttctg aaatacagaa cggttctttt tctttccggg atttttatac
     4621 ccgcaggatt aagcggattt atcctgcttt tattgcggcc gtgtcgctgg cttcggtgat
     4681 tgcctctcaa atcttccttt acgaagattt caaccaaatg cggaaaaccg tggagctttc
     4741 tgcggttttc ttgtccaata tttatctggg gtttcagcag gggtatttcg atttgagtgc
     4801 cgacgagaac cccgtactgc atatctggtc tttggcagta gaggaacagt attacctcct
     4861 gtatcctctt ttgctgatat tttgctgcaa aaaaacaaaa tcgctacggg tgctgcgtaa
     4921 catcagcatc atcctatttc tgattttgac tgccacatcg tttttgccaa gcgggtttta
     4981 taccgatatt ctcaaccaac ccaatactta ttacctttcg acactgaggt ttcccgagct
     5041 gttggcaggt tcgctgctgg cggtttacgg gcaaacgcaa aacggcagac ggcaaacagc
     5101 aaatggaaaa cggcagttgc tttcatcact ctgcttcggc gcattgcttg cctgcctgtt
     5161 cgtgattgac aaacacaatc cgtttatccc gggaatgacc ctgctccttc cctgcctgct
     5221 gacggcactg cttatccgga gtatgcaata cgggacactt ccgacccgca tcctgtcggc
     5281 aagccccatc gtatttgtcg gcaaaatctc ttattcccta tacctgtacc attggatttt
     5341 tattgctttc gcccattaca ttacaggcga caaacagctc ggactgcctg ccgtatcggc
     5401 ggttgccgcg ttgacggccg gattttccct gttgagttat tatttgattg aacagccgct
     5461 tagaaaacgg aagatgacct tcaaaaaggc atttttctgc ctctatctcg ccccgtccct
     5521 gatacttgtc ggttacaacc tgtacgcaag ggggatattg aaacaggaac acctccgccc
     5581 gttgcccggc gcgccccttg ctgcggaaaa tcattttccg gaaaccgtcc tgaccctcgg
     5641 cgactcgcac gccggacacc tgcgggggtt tctggattat gtcggcagcc gggaagggtg
     5701 gaaagccaaa atcctgtccc tcgattcgga gtgtttggtt tgggtagatg agaagctggc
     5761 agacaacccg ttatgtcgaa aataccggga tgaagttgaa aaagccgaag ccgttttcat
     5821 tgcccaattc tatgatttga ggatgggcgg ccagcccgtg ccgagatttg aagcgcaatc
     5881 cttcctaata cccgggttcc aagcccgatt cagggaaacc gtcaaaagga tagccgccgt
     5941 caaacccgtc tatgtttttg caaacaacac atcaatcagc cgttcgcccc tgagggagga
     6001 aaaattgaaa agatttgccg caaaccaata tctccgcccc attcaggcta tgggcgacat
     6061 cggcaagagc aatcaggcgg tttttgattt ggttaaagat atccccaatg tgcattgggt
     6121 ggacgcacaa aaatacctgc ccaaaaacac ggtcgaaata cacggacgct atctttacgg
     6181 cgaccaagac cacctgacct atttcggttc ttattatatg gggcgggaat tccacaaaca
     6241 cgaacgcctg cttaaatctt cccacggcgg cgcattgcag tagcctgcct tcttgtcgga
     6301 tattgccttt ggcagcctat gccgctgttt gccgttcggg gcggc
//
LOCUS       DAWKQJ010000111         6290 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000111 DAWKQJ010000000
VERSION     DAWKQJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.111"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(546..1130)
                     /gene="rnhB"
                     /locus_tag="WJL20_001527"
     CDS             complement(546..1130)
                     /gene="rnhB"
                     /locus_tag="WJL20_001527"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ7683668.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDTLAEMIKDQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLVVHPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1198..3093)
                     /gene="mnmG"
                     /locus_tag="WJL20_001528"
     CDS             complement(1198..3093)
                     /gene="mnmG"
                     /locus_tag="WJL20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ7683669.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3418..4845)
                     /locus_tag="WJL20_001529"
     CDS             complement(3418..4845)
                     /locus_tag="WJL20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7683670.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5005..5994
                     /gene="pdxA"
                     /locus_tag="WJL20_001530"
     CDS             5005..5994
                     /gene="pdxA"
                     /locus_tag="WJL20_001530"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ7683671.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHILAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRRERINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1321 a         1638 c         1828 g         1503 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aatcaaaaac acaaaactga aatatcgtca ttcccgcgca
       61 ggcgggaatc taggtctgtc ggtacggaaa cttatcggga aaaacggttt ttccaaccct
      121 gagactccgg attcctgttt tcgcaggaat ccggtttttt gagtttcagt cattttgata
      181 aattcttgca gctttgggtt tctagattcc cgcttttgcg ggaatggcgg tttggaagtt
      241 acctgaaatt caaaaaaacg gaaaccggac ggactggatt cccgttttca cgggaatgac
      301 ggattttagg tttctttttt gattttctgt ttttcgcggg aatggcggtt tggaagttac
      361 ctgaaattca aaaaaaaaaa acggaaaccg gacggattgg attcccgcct gcgcgggaat
      421 gacggatttt aggttttttt ttgattttct atttttcgcg ggaatgacgg tttgggttct
      481 ttctctttgg agttgcgatg ccggaaatgc cgtctgaagg cttcagacgg catttttgtg
      541 ccggtttaaa acaaggcctg ctgcgcgagc aggtttctga cgggggcgaa gtcgcggcgg
      601 tgttcgggca gcacgccgta ttgtttgagg gcttccagat gctgcttcgt gccataacct
      661 ttgtgtttgt cgaaaccgta ttggggatgg cgttgcgcca gtgcgtacat ttctgcatcg
      721 cgtgcggtct ttgccaaaac ggatgcggcg gagatttcga tgattttgct gtcgcctttg
      781 acgacggctt cggcagggat acctaaatgt tctggaatgc ggttgccgtc gatgaatatt
      841 ttttcggggt gcacaaccaa accgtcaacg gcgcgtttca tggcaagcat ggtggcgtgc
      901 aggatgttga ggctggcgat ttcttcgggc gtggcggcgg cgacgtgcca cgcggccgcc
      961 tgatctttaa tcatttcggc aagcgtgtcg cgttttttct cgctgagttt tttggagtcg
     1021 gtcagtccgg gtaggtcaaa cgtttccgga aggatgacgg cggcggcaaa cacgctgccg
     1081 actaaaggtc cgcgtccggc ctcgtccacg ccggcggtca gtatgtgcat gatgtttcct
     1141 gtcgggatgg tacgatgccg tctgaaaagg gtttcagacg gcatcgtacc gatgtgttta
     1201 tttcgcgtct ttaaacccgc gcttcaaatg caccatcagc agtgcgacgg cggcaggcgt
     1261 tacgccggaa atgcggcttg cctgcccgac ggtttcgggt ttgtgctggt tgagcttttg
     1321 ctgcacttct gccgacaagc ctttgacttt gccgtaatcg atgccgtcgg gcagttttaa
     1381 ggtttcgatg tcgcggcggc tgtcgatttc ttcgttttgg cggtcgatat agccttggta
     1441 tttgacttgg atttcgactt gttcgatgac ttcggcggag aggttttcag acggcatcgc
     1501 gccttcgagc gtcatcagcg cggcgtagtc gaggttcggg cggcgtaaca aatcgtgcag
     1561 gttggcttct cggctgagtt tttgtccgaa tacgcggatt tgttcgtctt cggcgagttt
     1621 ttgcggtgtg taccacgttg ttttcaaacg ttggatttcg cgttcgacgg cttcgcgttt
     1681 ttcgttgaac atacgccatt gcgcttcacc caccaagccg attttgtagc cgtcttcggt
     1741 caggcgcatg tcggcgttgt cttccctgag ttgcaggcgg tattcggcgc ggctggtgaa
     1801 cattcggtag ggttcgttca cgcctttggt gatgaggtcg tccaccaata cgccgaggta
     1861 ggcttgttcg cggcgcagca ggagcgggtc ttgtccgcgc acatattgca cggcgttcgc
     1921 gcctgccaat aaaccttgcg cggcggcttc ttcgtagccg gtcgtaccgt tgatttgccc
     1981 agcgaaaaac agtccctcaa tggttttggt ttcgaggctg gctttgaggt tgcgcgggtc
     2041 gaagtagtcg tattcgatgg cgtagccggg gcgcaggata tgggcgtttt ccaaaccttt
     2101 catactgcgg acgagcgcga tttggatgtc gaacggcagg ctggtggaga taccgttagg
     2161 atagtattcg tacgtagtca ggccttcggg ttctaggaaa atctggtggc tgtctttatc
     2221 ggcgaaacgg ttgattttgt cttcgataga cggacaataa cgcgggccca cgccttcgat
     2281 tttgccggta aacatcgggc tgcggtcgaa gcctgagcgg atgatgtcgt gggtttgcgt
     2341 gttggtatgc gtaatccagc aggacacttg gcgcgggtgc atttcggcgt tgccgcgcac
     2401 ggacatgacg ggaacgggtg tgtcgccggg ctgttcggtc agttgggaaa agtcaatcgt
     2461 gcgtccgtcg atgcgcggcg gcgtgccggt tttcagacgg ccttgcggca gcttcaattc
     2521 gcgcaaacgt ccgcccagcg atttggcggc ggggtcgccg gcgcgtccgc cctcgtagtt
     2581 ttccaaaccg atgtggattt tgccggacaa aaacgtgcct gcggtcaaca ccacggcgcg
     2641 tgctttaaac tccacgccca tcgcggtgat tacgcctgaa atgcggtcgc catcgagcgt
     2701 tacgtcttcg acggcttgtt ggaaaaggtc gaggttttct tggttttcca acatttcgcg
     2761 gatggcggct ttatatagga tacgatccgc ctgcgcgcgc gtggcccgta ctgccgcgcc
     2821 tttgctggcg ttcagacggc ggaactggat gccggatttg tcggttgcca acgccatcgc
     2881 gccgccgagc gcgtcgagtt cgcgcaccaa atgccctttg ccgatgccgc cgatggaagg
     2941 gttgcacgac atttgtccga gcgtttcaat attgtgtgtg agcaaaagcg tctgcgcgcc
     3001 catacgggcg gcggcgagcg cggcttctgt gccggcgtgt ccgccgccga cgacgataac
     3061 gtcgtaggtt ttggggtaaa tcatgtgggt catgtgtgta ttgcctgtcg gtgtttcaga
     3121 cggcatttat agtggattaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag
     3181 aacgattctc taaggtgctg aagtaccaag tgaatcggtt tcgtactgct tgtactgtct
     3241 gcggcttcgt cgccttgtcc tgatttttgt taaaccacta tattcaatat gccgtctgaa
     3301 aaacgaaatg gattcaaaag taaagggttg ggattgtacg cttgttcgcc ctgtttttac
     3361 agtgtgcgga aagggaaaag ccgcttcgcg gggaagcggc tccggtaagg gcgggattta
     3421 ccaaacgtcg gatttgatac ggcgtttcag gcccggatgt tcggaaagtt tgaactcggg
     3481 gtctttgccc attttcagct tggcggtgta atcgcgcagc agcataaacg ccaagggcga
     3541 gagcagcagg atggcgacaa ggttgatcca cgccataatg cccatcgcca tatccgccat
     3601 atcccagacc aaaggcacat tggcaaccgc gccgaaatag acccacgcca aaaccagcat
     3661 acggaaaacg gcggtaatca gccaatggct tttgatgaat tggacgttgg actcggcata
     3721 ggcatagttg ccgataacgg tggaaaaggc aaacataaac aggatgacgg cgaggaagcc
     3781 cgcgccccat tgccccactt ggctgacaat cgccgcctgc gtcaacgccg caccgctcaa
     3841 atcgccgtaa ggctgttggt aaatcaagat gatgaaggcg gtgcaagaac aaacgatgat
     3901 ggtatcgaca aacacgccca gcatttgaat cataccttgc gaaacagggt gtttcacttc
     3961 ggcggcggcg gcggcgttcg gcgcggaacc catacccgcc tcgttggaat acaggccgcg
     4021 tttgatgccc atcatcatcg tttgcgaaat cagaccgccg agtaagccgc ctgctgccgc
     4081 gtcgaatttg aacgcgcccg aaaaaatctg accgaacacg tccggaatca tcggaatatt
     4141 ggtcaaaatg atgaaaagcg cgataaagag gtacaaaacc gccatcaggg ggacgacgat
     4201 ttccgccgct ttagatatgc gcctgatgcc gccgaagata atcggcgcgg ttaaaatcac
     4261 cagggcgacg ccgacataat gaggctccca accccacgcc gctttgacgg tatcggcaat
     4321 ggtattggtc tgaaccgctt caaacacaaa gccgaaacag aaaatcaggc tcagggcgaa
     4381 caacacgccc agccatttct gccccagccc ttgagtgatg tagtaggcag ggccgccccg
     4441 gaaatggtgg ttgtcgtagt cgcggacttt aaagagctgc gccagcgaag attcgacaaa
     4501 cgccgaactc ataccgatta aggcggttac ccacatccaa aacaccgcgc ccggtccgcc
     4561 gactttgatg gcgatggcca cgcccgcgat attgcccacg cccacgcggc tggcaaggcc
     4621 ggttacaaat gcctgaaacg gcgtgatgcc gtgagggtcg tccccctgtt tgcggccgcc
     4681 gagcatttct ttgatgctgc gcccgaacag gcggaattgg acaaagcccg tggttacggt
     4741 gaagaaaagc cccgtaccca aaagcatata aaccaagtat gaccacatcg gatcgttgat
     4801 ggcgccgacc cagccgtgca gccattcggt aaagttctcg ttcatatcgc ttccttaaag
     4861 ttgaaacccg cacatattgg cggtatgcaa gcagggttta aattttgtaa acgcccattc
     4921 tagcagattg tcaacaaaat cagaaaaatt tacatcgccg cgcggctgcg gcgttagaat
     4981 cgcattttgt ttggagcaaa cacgatgaaa cagcctgttt ttgccgttac ttccggcgag
     5041 cctgccggca tcggccccga tatttgtttg gacttggcgt ttgcacgcct gccctgccgc
     5101 tgcgcggtat tgggcgacaa aaacctattg cgcgcgcgcg ccgaagcctt gggcaaaagc
     5161 gtcgtcttgc gcgacttcga tccggaatca ggcggcgcgg catacggcga gctggaagtg
     5221 ctgcacatcc ttgccgccga agcggttgag gcagggcggc tcaatcccgc caacgccgcc
     5281 tatgtgctgc aacttttgga caccgcgctc gcaggcattt cagacggcat tttcgacggc
     5341 atcgttaccg cgccgctgca caaaggcatc atcaacgacg cgcgcgcaag cacaggtttt
     5401 ttcagcggac acaccgaata tctggcggaa aaaagcggca cggggcaggt cgtgatgatg
     5461 cttgccggca aaggcctgcg cgtcgccctc gtaacgaccc acctgccgct gaaagacgtt
     5521 gccgccgcca tcacgcaacc gctgattgaa agcgtcgcac gcattttgca tcacgactta
     5581 aaacacaaat tctgcatcaa aaatcccaaa atcctggtcg ccggactcaa cccccacgcc
     5641 ggtgagggcg gacacctcgg acacgaagaa accgacacca ttatccccgc gctcgaaaac
     5701 ctgcgccgcg aaaggataaa cctcgccggc ccgtatccgg cggacacatt gttccaaccg
     5761 tttatgctcg aaggtgcgga tgccgtattg gcgatgtacc acgaccaagg gctgcccgtg
     5821 ttgaaatacc acagtttcgg gcagggcgtg aacatcacac tggggctgcc ctttatccgc
     5881 acctccgtcg atcacggcac cgcgctcgat ttggcggcaa ccggcagggc ggattccggc
     5941 agcctgataa ctgccgtgga gaccgccgtc gagatggcgc gcggcagcct ttaaagatga
     6001 taaaagaccc gtcatttccg cgcaggcggg aatccggtct gttcggtttc agttgttttt
     6061 gggtttcggg taatttccaa atcgtcattc ccgcgcaggc gggaatccag accttcgggc
     6121 gacggcaata ttcaaagatt atctgaaagt ttgaggttct agattcccgt tttcacggga
     6181 atgacgaaag gttgcgggaa tccggcttgt tcggtttcgg ttttttttga ggtttcgggc
     6241 aacttctaaa ccgtcattcc cgcgcaggcg ggaatccagg cctttgggcg
//
LOCUS       DAWKQJ010000112         6276 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000112 DAWKQJ010000000
VERSION     DAWKQJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.112"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            61..444
                     /locus_tag="WJL20_001531"
     CDS             61..444
                     /locus_tag="WJL20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ7683672.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            559..1032
                     /locus_tag="WJL20_001532"
     CDS             559..1032
                     /locus_tag="WJL20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ7683673.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1158..2261
                     /gene="mnmA"
                     /locus_tag="WJL20_001533"
     CDS             1158..2261
                     /gene="mnmA"
                     /locus_tag="WJL20_001533"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ7683674.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2332..4002
                     /locus_tag="WJL20_001534"
     CDS             2332..4002
                     /locus_tag="WJL20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ7683675.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4114..5748
                     /locus_tag="WJL20_001535"
     CDS             4114..5748
                     /locus_tag="WJL20_001535"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ7683676.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5763..5965
                     /locus_tag="WJL20_001536"
                     /pseudo
     CDS             5763..5965
                     /locus_tag="WJL20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5976..6276
                     /locus_tag="WJL20_001537"
                     /pseudo
     CDS             5976..6276
                     /locus_tag="WJL20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1640 a         1735 c         1556 g         1345 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcca ctataagatt aagcacattc cccgtctgat
      541 attataagga gcagaaagat gaaaaaatta ttggcggccg tgatgatggc aggtttggca
      601 ggcgcggttt ccgccgccgg agtccacgtt gaggacggct gggcgcgcac caccgtcgaa
      661 ggtatgaaaa taggcggcgc gttcatgaaa atccacaacg acgaagccaa acaagacttt
      721 ttgctcggcg gaagcagccc cgttgccgac cgcgtcgaag tgcataccca catcaacgac
      781 aacggcgtga tgcggatgcg cgaagtcgaa ggcggcgtgc ctttggaagc gaaatccgtt
      841 accgaactca aacccggcag ctatcatgtg atgtttatgg gtttgaaaaa acaattaaaa
      901 gagggcgata aaattcccgt taccctgaaa tttaaaaacg ccaaagcgca aaccgtccaa
      961 ctggaagtca aaatcgcgcc gatgccggca atgaaccacg gtcatcacca cggcgaagcg
     1021 catcagcact aatctgctga aaatgtttga aatgccgtct gaaaaagccc gggctttcag
     1081 acggcatttt tatgcccgcc tttaaaatgt gttaaaatcc gcctttaaaa accgccgttt
     1141 ccaaccatat tctacgcatg aatacgacag caaacccttc caatatcatc gtcgggctct
     1201 ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt tatcaagtgc
     1261 gcggtgtgtt catgcagaac tgggaagacg acgacaacga cgaatattgc agcatcaaac
     1321 aggattcgtt cgatgccatc gccgttgccg atattatcgg catcgatatc gacatcgtta
     1381 atttcgccgc gcaatataaa gacaaagtct ttgcttattt tcttcaggaa tacagtgcgg
     1441 ggcgcacgcc gaatccggat gtgttgtgca acgccgaaat caaattcaaa tgctttttgg
     1501 actacgccgt agggcagggc gcggatacca tcgccaccgg ccactatgcg cgcaaagaag
     1561 ttcgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac caaagctatt
     1621 tcctctaccg cctcaagcct ttccaactcg aacgcgcgat ttttccgttg ggcggtttgg
     1681 aaaaacccga agtgcgccgc cttgccgccg agttcaaact gccgaccgcc gccaaaaaag
     1741 acagcacggg catctgcttc atcggtgaac gcccgtttcg cgagtttctg caaaaatacc
     1801 tgccgaccga caacggtaaa atggttacgc ccgaagggaa aaccgtcggc gaacacgtcg
     1861 ggctgatgtt ctacacgctc ggacagcgca aaggtttggg catcggcggc gcgggcgaac
     1921 cgtggtttgt tgcggctaaa gatttgacga aaaacgaact gattgtcgtg caaggacacg
     1981 accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc acgcttcccg
     2041 aacgcccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg gacgcgcctt
     2101 gtgagttgcg ctatttggat gaagaaaccg ccgagctggt gtttgacgaa ccgcaatggg
     2161 cggttacgcc gggccagtct gccgtgctgt acgacggcga catctgtttg ggcggcggca
     2221 tcatccaaac gaccgacaaa cccgtcatca tcacacgata aaggtaatgc cgtctgaaac
     2281 ggttttcaga cggcattgtt ccgctcaatt ccactttaaa gaccgatacc tatggaaaaa
     2341 atctggttag acagctacga gaagggcgtc agtgccgaaa tcgacatcac gcaatacaat
     2401 tccatcagcg acgtattccg ccaaagcgtg gaaaaatttg cccgtctgcc cgcttttcaa
     2461 aatatgggca aaacgctgac ttatgccgaa accggcaaac tggcgactga tttcgcctct
     2521 tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg ttgccattat gatgccgaac
     2581 gtattgcaat acccgatagc ccttttcggc attttgcagg caggtttggt ggcggtgaac
     2641 accaatccgc tctatacgcc gcgcgagctg gagcatcagc tgaaagacag cggcgcgacc
     2701 gccatcatcg ttttggaaaa ttttgccaac acgctggagc tggtgctgcc gcgcacgcag
     2761 atcaaacacg tcatcgtcgc ctccgtcggc gaaatgttcg ggctgcttaa aggttcgctg
     2821 atcaatttca tcatccggaa aatcaagaaa atggtgcccg aataccgtat tcgggaaacc
     2881 gtttcctttc agacggcatt gaaagaggga gcgaagcacg ttttccaacc tgtcgcatta
     2941 aaccgcgaag ataccgcgct gctgcaatac acgggcggca cgacaggtgt tgccaaaggg
     3001 gcggtgctga gtcacggcaa catctgcgcc aatatgcttc aggcaaaaga atggattaaa
     3061 aaccagttgc gtgagggaaa agaaaccgtt atcgccgcct tgccgctgta ccatattttt
     3121 gccttaaccg tgaatctgat gatttttgcc aatgccggct cgaaaatcat cctgattacc
     3181 aacccgcgcg atatgaaagg ctttatcgga caactgaaaa aacagcgggt taatgtatgt
     3241 atcggcgtga acacactgtt taacgcaatg gttaaccgtc ctgattttgc cgaagtcgat
     3301 ttttcaggat tgcggctgac tttgggcggc ggtatggcga cccaaaaagc cgttgccgaa
     3361 aaatggaaaa aaatcaccgg cacgcccatc gtcgaagcct acggtctgac cgaagccagc
     3421 cccggcgtgt gctgcaaccc cttaaacatc gaatcataca gcggcagcat cggtttgccc
     3481 gtctcgtcca ccgaagtcga actgcgcgac gcaaacggca aagaagtccc cgtcgggcag
     3541 ccgggcgaat tgtgggtaaa aggccctcaa gtgatgcaag gctactggaa ccgccccgaa
     3601 gaaaccgcca aagccataga cgcgcgcggc ttcttggaaa ccggcgatat tgccgtcatg
     3661 gacgaaaaag gctggttgaa gctggtcgat cgtaagaaag acctcgtcgt tgtttccgga
     3721 ttcaatgttt atccgaacga aatcgaggaa gtcatcgcgc atcacggcaa agtcatggaa
     3781 gtcgcctgta tcggcgttcc cgacgaaaaa accggcgagg cactcaaagt attcgtcgtc
     3841 aaaaaagacc cgtcattgac caaagaagaa cttatcgcct tttgccgcac cgaattaacc
     3901 gcatataaag taccgaaaaa tatcgaattc cgcgacgagt tgcccaagtc caacgtcggc
     3961 aaaatcctgc gccgcgagtt gcggcaaagt accgggaaat aaagaaaaga tgccgtctga
     4021 aaacagccgt ccaccgttca gacggcatcc gtccgtttgc cagaaccgcg cgcttcacgt
     4081 taaaatcacg cattccaaca tgggtattcc atcatgacca aattcatttt cgtcaccggc
     4141 ggcgttgtct cctcactggg taaaggtatc gccgccgctt ctattgccgc catcctcgaa
     4201 tcgcgcggct tgaacgttac catgctcaag ctcgatcctt atatcaacgt cgatcccggc
     4261 acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg acgacggcgc ggaaaccgac
     4321 ctcgacttgg gacactacga acgctttatc gattccacga tgacccgccg caacagcttc
     4381 agcacgggac aggtgtacga aaacgttatc gccaaagaac gacggggcga ctacctcggc
     4441 ggtacggttc aagtcatccc gcacattacc gacgaaatca aacgccgcat ccacgaaggc
     4501 gcggcaggtt acgatgtggc gattgtcgaa atcggcggta cagtcggcga tatcgaatct
     4561 ttgccattct tggaagccat ccgccaaatg cgcagccagc tcggccgcaa caatacattg
     4621 tttgcccacc tgagctatgt tccttacatc gccgctgcag gcgaaatcaa aaccaagccg
     4681 acccagcaca ccgttaaaga aatgttgagc atcggtttgc aacccgacat cctgatttgc
     4741 cgtatggaca ggacaatgcc tgcggacgaa cgccgcaaaa tcgccttgtt ctgcaacgtg
     4801 gaagaacgcg cgattgtcgg cagctacgat gtggacagca tctacgaatg ccccgaaatg
     4861 ctgcacgacc aaggcatcga taacatcatt accgagcagt tgcagcttaa tgtgcagcag
     4921 gcggatttga ccgcgtggaa aaaaatcgtc cacgccatcc aaaacccgaa acacaccgtc
     4981 aaaatcgcca tggtcggcaa atacgtcgat ttgaccgagt cctacaaatc gctgatcgaa
     5041 gccttgaaac acgcaggcat tcacaccgaa accgacgtac aaattacctt tgttgacagc
     5101 gaaagcatcg agaaaaacaa cggcgacgtt tctatgctca aagatatgga cgccatcctc
     5161 gttcccggcg gtttcggttc gcgcggcgtg gaaggcaaaa tcgccgctgt gcgctatgcc
     5221 cgcgaaaaca acgtgccata cttgggcatc tgcctcggta tgcagattgc gctgatcgaa
     5281 tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca ccgagttcga cctcaaatgc
     5341 gccgcccccg tcgttgccct gattgacgaa tggcaaactg ccgacggcag cgtcgaaacc
     5401 cgcgacgaat ccgccgattt gggcggcacc atgcgtttgg gcgcgcaaga agtcgaattg
     5461 aaagcaggca gcctcgccgc caaaatctac ggcagcgaac acatccgcga acgccaccgc
     5521 caccgctacg aagtcaacaa caactacgtt cctacgctgg aacaggcagg tttggtcatc
     5581 ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca tcgaactgcc gaaccatcct
     5641 tggttcttcg cctgccagtt ccaccccgag tttacctcca atccgcgcaa agggcatcct
     5701 ttgttcaccg cgtttgtcaa agctgcgttg aacaataaaa aagcctgata aagcgttact
     5761 tgatgatcaa aatgccgtct gaaagcctga atgaagactt tcagacggca ttttcgcaaa
     5821 tcggggatta taaaagccat caatttaatt tttatggttt tatctaatat ttaccaactg
     5881 aatattgtta agcggggcgt gaaactcaag ttgagacctt tgcaaaattc cccaaaatcc
     5941 cctaaattcc caccaagaca tttaggggat ttctcatgag cacctccttt caacaaaccg
     6001 cccaagccat gattgccaaa cacatcgacc gcttcccact attgaagttg gactaggtga
     6061 ttgattggca gccgatcgaa cagtacctga atcgtcaaag aacccattta ccttagagac
     6121 caccgcggcc gtccgcctat ccgctgctat ccatgttcaa agccgtcctg ctcggacaat
     6181 ggcacagcct ctccgatccc gaactcgaac acagcctcat cacccgcatc gacttcaacc
     6241 tgttttgccg ttttgacgaa ctgagcatcc ctgatt
//
LOCUS       DAWKQJ010000113         6245 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000113 DAWKQJ010000000
VERSION     DAWKQJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.113"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..130)
                     /locus_tag="WJL20_001538"
     CDS             complement(<1..130)
                     /locus_tag="WJL20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ7683677.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVV"
     gene            252..1205
                     /locus_tag="WJL20_001539"
     CDS             252..1205
                     /locus_tag="WJL20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7683678.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1280..3337)
                     /gene="metG"
                     /locus_tag="WJL20_001540"
     CDS             complement(1280..3337)
                     /gene="metG"
                     /locus_tag="WJL20_001540"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ7683679.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKTGGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3453..5291)
                     /gene="glmS"
                     /locus_tag="WJL20_001541"
     CDS             complement(3453..5291)
                     /gene="glmS"
                     /locus_tag="WJL20_001541"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ7683680.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLVEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            5573..6091
                     /locus_tag="WJL20_001542"
     CDS             5573..6091
                     /locus_tag="WJL20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683681.1"
                     /translation="MKQMLWAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIDHQKKCGQTEQAYRDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1330 a         1463 c         1900 g         1552 t
ORIGIN      
        1 aaacgacggt gttgattagg ggttcgacca gcgcgaccgc cccggatatg cctatactgc
       61 ccgtcagtac ataggttaca ctgaaggcgg tgctgaaatg cagtgcggca aaagtcaggg
      121 ttttaagcat catcctctcc cgaattggac attgacggag gaatgataaa ggttatcata
      181 aggttgcgcg gtttaaattt gctatttatt gttagcgtaa ataaatcatt ttccaaataa
      241 ggatagaaat tatgaatcat aaaaagatcg ttgttttgga tgcggatact ttgcccggtc
      301 gggtctttca ttttgatttt ccgcacgagc ttgcggttta cggtacgaca ggtgcggatg
      361 aaacggcgga acgggtgcgc gacgcacata ttgtcattac caacaaagtg atgatttctg
      421 ccgatattat tgcggctaat ccgcagttgg agctgattgc cgtcagtgcg accggtgtga
      481 acaatgtcga tattggggcg gcgaaggcgg cgggcgttgc ggtatgcaat gtccgcgcct
      541 acggaaacga atcggttgcg gaacacgcct ttatgctgat gattgcgttg atgcggaatt
      601 tgcccgccta tcagcgtgat gttgcggcag gattatggga aaagtcgccg tttttctgcc
      661 attacggtgc gccgattcgg gatttgaacg gcaaaacgct ggcggttttc ggacgcggca
      721 atatcggacg gacgcttgcc ggatacgcgc aggcattcgg tatgggggtg gtgtttgccg
      781 aacacaaaca cgcgtccgct gtgcgtgaag gctatgtttc ctttgaagat gcggtacggg
      841 ctgctgatgt gttgtcgctg cactgtccgc taaacgccca aactgaaaat atgataggcg
      901 aaaacgaatt gcggcagatg aagcctggcg cggttttaat caattgtggg cgcggcgggc
      961 tggtggatga aaacgcgctg cttgccgcac tcaaatacgg gcagatcggc ggggctggtg
     1021 tcgatgtttt gacggaggag ccgccaaggg gcggcaatcc cctgctgaat gcacgacttc
     1081 ccaatctgat tgttacgccg cataccgcgt gggcgagccg cgaggctttg gacaggctgt
     1141 tcgatatatt gttggcgaac attcacgcct ttgtgaaagg cgaggcgcaa aaccgggtag
     1201 tttgagcttg tcgggataac gaaaaacaat gccgtctgaa cgcccaaagg gttcagacgg
     1261 cattttttga gatttctact taaccgactt tgtcgcccgg ttgcgcgcct gcatccacat
     1321 ccaagagctt cagtttcccg tctgccgtgg cggcactcaa aatcatgcct tcggatacgc
     1381 cgaattttgc cattttgcgc ggggcgaagt tggcgacggc gatgaccatt cttccgttta
     1441 actcggcagg gttggggtaa aacgcggcga tgccggagaa gatgatacgt ttttcaaaac
     1501 cgaaatcgag gtcgaatttt aagagtttgg tgctgccttc gacggcttcg cagttcaata
     1561 ctttggcgac gcgcatgtcg attttcataa agtcgtcgaa actcgcctgt tcggcaactt
     1621 tttcgtattt gccctcttcg gcggcaggca cggctgcggc ggcgatgctt tgtttgttgg
     1681 cttcgattaa atcgtccact tgtttttgct ccactcgttg cattaaatgt tcgtatttgt
     1741 tgatggcgtg tttgcccaag gtttcgcgtg tatttgccca agtgatggct tccaaattca
     1801 ggaatttggc ggcattttcg gccactttcg gcaacacggg ggcgaggtag gcggtcagca
     1861 tggtaaaggc gttgatgagt tcgctgcata cttcgtgcag gcgttcgtct tggccttctt
     1921 gtttggcgag ttcccaaggt ttgttggcat caacgtattc gttgacaatg tctgccaagg
     1981 ccatgatgtc gcgcagggct ttggcgtatt cgcggctttc gtagcattcg gcaatggctt
     2041 cgctttgtgc ggtcagtttt gccaataatg cgctgtcggc gacgtctttc aaacggcctt
     2101 caaagcgttt ggcaatgaaa cctgacgcgc gggcggcgat gttgacgtat ttgccgacga
     2161 ggtcgctgtt gacgcgcgag ataaagtctt gcaggttcaa atcgatgtct tcgattttgc
     2221 tgttgagttt ggcggcgatg tagtagcgca tccactcggg gtttaggcct tgttccagat
     2281 aggattttgc ggtgatgaag gtgccgcgcg atttggacat tttttgtccg tcgacggtca
     2341 aaaagccgtg tgcgtacacg ccggtcgggg cgcggtggcc ggagaaatgc agcatagcgg
     2401 gccagaacag ggcgtggaaa tagaggatgt ctttgccgat gaagtggtac atctcggttt
     2461 ggctgccggc tttgaagtat tcgtcaaaat cgatgccgat gcggtcgcac aggtttttaa
     2521 acgatgccat gtagccgacg ggcgcgtcca gccagacgta gaagtatttg cccggagcgt
     2581 cggggatttc aaaaccgaaa tacggcgcgt cgcgggaaat gtcccagtcg gacagggtgg
     2641 tttcttcgcc ttcgcccagc cattctttca ttttgttgag ggcttcgggt tgcagatggg
     2701 gcttgccgtc gtgcgggttg ttgccggaag tccatgcttt gaggaagtcg gcgcattcgt
     2761 ccagtttgaa gaagaagtgt tcggattcgc gcaattcggg tttggcaccg gaaacggcgg
     2821 aatacgggtt gatcagttcg gtcggggagt aggtcgtgcc gcagacttcg cagttgtcgc
     2881 cgtattggtc ttgagcgtgg catttggggc attcgccttt gacgaagcgg tcgggcagaa
     2941 acatttgttt ttcggggtcg aaaagctgct cgatgacgcg gctctcgatt ttgccgccgg
     3001 ttttcagcgc gcggtaaata tcttgggaaa actgtttgtt ttcaggggaa tgggtgctgt
     3061 aataattgtc gtagccgatg ttgaaaccgg taaagtcggc gaggtgctct tcgcgcactt
     3121 tggcaatcat gtcttcaggg gcaatgcctt gtttttgcgc ggcaagcatc acgggcgtgc
     3181 cgtgggtgtc gtcggcgcag cagtagtagc attcgtggcc gcgcagtttt tggaagcgca
     3241 cccaaacgtc ggtttggatg tgttcgacca tgtgtccgag gtggatgctg ccgttggcat
     3301 agggcagggc ggaggtaacg aggattttgc gtgtcatatt gtgctttgca aacaatgggt
     3361 aaaggcggat tataccgcaa atcaaacggg gaaatgccgt ctgaagcctg aaaaatcagg
     3421 cttcagacgg catgttcgca aacaatccgg acttattcga ccgtaacgga tttcgccagg
     3481 ttgcgcggtt tgtctacatc ggtgccgcgt gcgagggcga catggtagga aaggagctgc
     3541 acggggatag tatgcacgac gggggacagt ttgccgacgt ggcgcggtgc gcggataacg
     3601 tgcacacctt cggtggcatt aaaattgctg tcgaggtcgg caaagacgaa aagttcgccg
     3661 ccgcgcgcgc cgacttcctg catattggct ttgactttgt ccaacaggct gtcgttgggt
     3721 gcgatgacga cgacgggcat gttttcgtcc accaaggcaa gcggcccgtg cttcagttcg
     3781 ccggcaggat aggcttcggc gtggatgtag gtgatttcct tcagttttaa cgcgccttcg
     3841 agggcaatcg ggtaatggat gccgcgcccc aaaaacagcg cgctggtttt cttggcaaac
     3901 tgttgcgccc aagcggcaat ttgcggttcg aggtttaaag cgtgctgcac gctgccggga
     3961 agctggcgga gttcttcggt gtaacgtgct tcgtcttctt cggaaaccaa accgcgcact
     4021 ttcgccagcg ttaccgccaa accgaacagc gcaaccagct gcgtggtaaa tgctttggtt
     4081 gaggcgacgc cgatttccgc accggcacgg gtataaagca cgaggctgct ttcgcgcggc
     4141 agggcggatt ccatcacgtt gcagatggag aggctgtggc ggtgtcccaa ggatttggcg
     4201 tatttcaacg cctccatcgt gtccagcgtt tcgccggatt gggaaatggt aatgaccagt
     4261 tggtccggat cggcaatcac gctgcggtat cggtattcgc tggcgatttc gacatcggcc
     4321 gggattttgg cgatggattc caaccaatat ttggcggtca gcgcggcgta ataggacgtg
     4381 ccgcaggcaa ggattttgac gctgcggatg ctttcaaaca cgcttttggc gtttttgccg
     4441 aagttttcgg ggatgaagcc gccgtcgagg aaaacctccg ccgtgtctgc aatcgcgcgc
     4501 ggctgctcat ggatttcctt ttgcataaag tggctgtacg gtcccagttc caaagaggcg
     4561 agcgagagtt cggatacctt gactttgcgt tcggcaggca ggccgttttt atcggtcagc
     4621 cttttgatgc cgtctgaagc cagcagcgcg atgtcgccgt cttcgaggta cgccacgcgg
     4681 cgcgtaaagg cgatgacggc ggatacgtcc gaagcgataa aggtttcatc gtcgcccaaa
     4741 gcgaccaaaa gcgggcagcc catacgcgcc acaactaatt catcaggctt gtcttgggca
     4801 ataaccgcga tggcgtatgc gccgtggaaa cgtttgaccg cttcctgcac agcttcaacc
     4861 aatttgccgc cgttttgcgc gtattcgtga ttgatgctgt gtgcgatgac ttcggtatcc
     4921 gtttgcgatt caaaacggta tcccaaacct tccaagcgtt tgcgttcgct ttcaaagttt
     4981 tcgatgatgc cgttgtggac aaccgcaatc atcccgccgc tgatgtgcgg gtgggcgttc
     5041 ggctcggtaa cgccgccgtg tgtcgcccaa cgcgtatgtc cgatgccgat gccgccgctg
     5101 atgccttttt cgcgtgctgc gtcttccata agctgcacgc gtccgacgcg gcgcacgcgt
     5161 ttgattttgc cgtcggtgtt gacggcaatg cctgatgagt cataaccccg gtattcgagg
     5221 cgtttgaggc cgtcggtcag aaaatcgacg acgttgtgat gggcgcggat ggcgccgacg
     5281 ataccgcaca taactgttcc ttagtatcca gttgaaaaaa aacaggcgcg gacggctccc
     5341 gtgccgcacc ttccgcttcg gattataaac cgccttccgc gccgaaaaac agcaaaatgc
     5401 cgtctgaagg cttgggcttg ctcaaaaaag ggagggattt ccccgcctga ccgggatggg
     5461 cgttcagacg gcattgcctg ctgccggttt atagtttttg taaatcaata ttgacaagct
     5521 gaaaaaaaaa aacaatatac tcgcccagtc ttaatgttaa cggagtatgg aaatgaaaca
     5581 aatgctttgg gccgtcggcg tggcggcggt gttggcgggc tgcggcaagg atgccggcgg
     5641 ttacgagggt tattggcgtg aaaagtcgga caaaaaagag ggtatgattg ccgtcaaaaa
     5701 agaaaaaggc aattacttcc ttaataaaat caacgtgttt acaggtaagg aagaatctat
     5761 gcttttgtct gaaaaagacg gcgcgctttc gataaacacg gggatagggg aaatcccgat
     5821 caaactttcc gacgacggga aagagctgta tgtcgaacgc aggcagtatg tgaaaaccga
     5881 tgcggcgatg aaggacaaaa tcatcgacca tcagaaaaag tgcggacaaa cggaacaggc
     5941 ataccgcgac gcgcgaaatg cgttgccgtc aaaccagacg tatcagcagc atcaggcggc
     6001 gatcgagcag ttgaaacggc ggtttgaagc cgagtttgac gaattggaaa aagaaatcaa
     6061 atgcaacggc aaaccgacat tgttgtttta gcgtaggaca accgggagga tgccgccgtc
     6121 cgaatcggac gtgcggtttc tgtaccggta cgggcgggca ggaatgtccg ctttttttgt
     6181 tcggacgcgt ttgaataccc gtttgattcc gtccgtttgc aaagggtatt tttgttcggg
     6241 cggaa
//
LOCUS       DAWKQJ010000114         6231 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000114 DAWKQJ010000000
VERSION     DAWKQJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.114"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            301..747
                     /gene="yciA"
                     /locus_tag="WJL20_001543"
     CDS             301..747
                     /gene="yciA"
                     /locus_tag="WJL20_001543"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ7683682.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(798..1619)
                     /locus_tag="WJL20_001544"
     CDS             complement(798..1619)
                     /locus_tag="WJL20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7683683.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1738..2196
                     /locus_tag="WJL20_001545"
     CDS             1738..2196
                     /locus_tag="WJL20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7683684.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2360..3475)
                     /gene="lst"
                     /locus_tag="WJL20_001546"
     CDS             complement(2360..3475)
                     /gene="lst"
                     /locus_tag="WJL20_001546"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ7683685.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3576..3680
                     /locus_tag="WJL20_001547"
                     /pseudo
     CDS             3576..3680
                     /locus_tag="WJL20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3678..3839)
                     /locus_tag="WJL20_001548"
     CDS             complement(3678..3839)
                     /locus_tag="WJL20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683686.1"
                     /translation="MAYFGGFVRALFDLGVFERGSVKGRGKWFSRVWWRSEVLCLMCG
                     GIFSVKLIL"
     gene            3908..6133
                     /locus_tag="WJL20_001549"
     CDS             3908..6133
                     /locus_tag="WJL20_001549"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ7683687.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1671 a         1821 c         1387 g         1352 t
ORIGIN      
        1 acggccccgc cctacaatac gccttgcgaa tctgtcccac gcccgccctt gtatgcctca
       61 aatgggttaa actacgcact tcccgacttc ccgtttcggg caggggtaca ggtccgattt
      121 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg
      181 gaaccgattc actcggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
      241 gtaacgccgt actggtttaa atttaatcca ctatatattt caataaaaca aggaaacttt
      301 atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg aactgctcct gcgtaccgtc
      361 gctatgccgc gcgataccaa tcccaaccaa gacattttcg gcggctggat tatgtcgcag
      421 atggatttgg gcggcggcat attggcggcg gaaatcgcgc ggggacgcat cgttaccgtc
      481 gccgttcagg aaatgaactt tatccgcccg gtcaaggtcg gcaacgtcgt ctgctgctac
      541 gggcattgcg tccgcgtggg caatacttcc ctccagctta aaatcgaagt ctgggtgaaa
      601 actttgatga acgattgcgt taccgaagac cgctacctcg taaccgaagc cgtgttcact
      661 tatgtcgcca tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg caaccccaaa
      721 ttggcgggct tattgcctac tccgtaaaaa tgcccgtaaa aatgccgtct gaaactgtct
      781 tcagacggca ttctgcatca tttgaaccaa gacctgatgc gggaaaacag cgacatactt
      841 tgtttttcaa agtcttcccg cacttcgggc acggcttttt ttatcacttt cattcctgca
      901 aaagtctgcg ccacgggcat aatttcaatc gtgttcacat tcatatgcgc cggccggcgg
      961 tacagccaca atgcggtttc cgcaatatct tcggggcgga taaattccac accctcatac
     1021 acgcctgctg ccttctcgtc atcgcctttg aagcgcacgt tggaaaactc cgtattgccg
     1081 cacaaacccg gctcgatatt ggtaacgcgg atgttcttat ccgccaactc cgcgcgcaaa
     1141 ttcaggctga actggcgcac aaacgccttg gtcgccccgt aaacgttgct gccggcataa
     1201 gcataattgc ctgcaatcga acccaaattc atcacataac cgccgccgcg ttccaccatt
     1261 tgcggcaaaa ttttgcgcgt caggaacgtc aaacccaaaa cattggtttg aatcatcgtt
     1321 tcccaatctt caaaatccgc cttgtccgcc gtgtccagac ccaaagccag cccggcattg
     1381 ttgatgaggc agtcgatgtc ggaaaattca tcggggatgc cgtttaaggc gttttccacc
     1441 gactcgcgtc gcgacacgtc catttccaaa gggtaaaaca aagcacccaa ttcatccgcc
     1501 aaggcctgaa gcctgtccgc acggcgcgcc gcaccgataa cgcggtatcc cgccccgaca
     1561 aacgcacggc acatcgcttc gccgaatcct gccgaagcac cggtaattaa aacagccatt
     1621 gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt atcataacgc tcttcagacg
     1681 gcatcgcgcc ggctgaaaat gacaacaacc ataccaccac gacaggacac attcaatatg
     1741 aaaacccaaa tcagccttgc tgctgccgcc atcacactgc tcctttccgc ctgcggaggc
     1801 agcggaatac cttcccaacc caaaggcgaa atttccgaaa accgcaccgc cgcgttcaaa
     1861 tccatgatgc ccgaattcac acgcatgggc aaaatggtca aagacgaaga accttacgat
     1921 gtcgaaaaat tcaaacaggc tgccgcggcg tttgccgaca gcagcaagaa accgttcaca
     1981 ctttttgagt ccgatccgca aggcaacggc cgcgccctgc ccgccgtttg gtcggatggt
     2041 gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg tcgaaaaact caacgccgca
     2101 gcacaaaccg gcaaactgga tgaaatcaaa gccgcctacg gcgaaaccgg cgcaagctgc
     2161 aaatcctgcc acgacagttt ccgcgcgccg gaataacatc cgaaacagaa cgatgccgtc
     2221 tgaagccttc agacggcatt ttctttgtca tttcagcaca cgcaaagcaa tcagaaatta
     2281 tcttcaattc aaaaaatacg ccatatcctg atttttgcct tacttactcc gccattttct
     2341 attttgattt gctatgcgat taatctttat cgtcaaatgt caaaatcggg atgccggatt
     2401 gggtaaacag ggcatacacc ggcttgagcc aataatcttc cggaagggaa gctggtttca
     2461 atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa cgccgcgccg ctgaaaaagg
     2521 tataaatttc atacctcgta tgcgggtttt tcttaatctc gcgcaaaata tagtcttcga
     2581 tgacataggg cgaatttaat gtggttacgc cggaaagccc gtaggtttgg cgggggtgcg
     2641 gtgcgacata ttgtatgttg aagttttttg ccgccttttc cgaaatttcc ttcatctcct
     2701 tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt ttcgtccccc gccttcagtt
     2761 cggacgcatc gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc ataatgtttt
     2821 tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt acgggttttg gcgatgctcc
     2881 aatctccgat catcatccgg gcaaaattcc gcttgatcgt cccgtttaca gaaaactcat
     2941 cacccaaata gctgctgctt tgaattaaat tgattgtccc gtcgtcaaag gttttgattt
     3001 ccgcatccgg gtaagtgctc aaaaaggcgg caatgctgac tttttccaaa cttgccaaat
     3061 aaatccgctt gaccttcggc agcagcattg ccttcacctt cagctccgcc atcgtcggaa
     3121 tgaaattaaa cgatttgttc aaaccgtagg gcaggtggaa aaaatacgcc cactccgcct
     3181 tatcctttat ctgattgaaa taataatcgt atttttcatt cctgttttca gacatcagca
     3241 ccacataaaa ccgctccccc ggatgctgcg ccataatcct ttctgccacc ttcatctgca
     3301 atatggtata gcagaaaatc agattgaccg gttccccctc ttcattgaag agtttgtcct
     3361 tcagcaggga aaccgcattc ctttccccct gatttacccg gtcaaatgta taaaatatcc
     3421 cgaaacaaaa aacaatcagg cacaacacgg tcaaacaagc ctttttcaag cccatatccc
     3481 taaaactcca ttccgacaaa ttgaacatac gccccgagtg tcccgggacg gaaaaatgcg
     3541 gaattttagc agatattccg acagttaccg aatattatag tggtttaaat ttaaatcagg
     3601 acaaggcgac gaagccgcag acagtacaag cagtacgaca aggcgaggca acgctgtact
     3661 ggtttaaatt taaaccacta taaaatcaat ttcaccgaaa atatgccgcc gcacatcagg
     3721 cacagcacct ccgaccgcca ccaaacgcgg gaaaaccatt tcccccttcc tttcacgctg
     3781 ccgcgttcaa acacgccaag atcgaacaaa gcgcgaacaa agccgccaaa atatgccata
     3841 atagccgcgc acattccccg cgcaatgtgt tccaaaagaa aaaaccaacc tacaagagaa
     3901 taatcctatg actcaaaaat ccaccattgt ttatacccat accgacgaag cccccgcgct
     3961 ggcgacccaa tcgctgctgc cgattgtgca ggcttttacc cgccacgccg atattgatgt
     4021 caaaactgcc gacatttccc tgtccggccg tattttggcg gcattccccg aatatctgac
     4081 cgaagcgcaa cgcgtacccg acgcgcttgc cgaattgggc gaactggtaa aacaacccga
     4141 tgcaaacgta atcaaactgc cgaacatcag cgcatccgta ccgcaactga ccgccgcgat
     4201 taaagaattg cagtctaaag gctttgccgt tcccgattat cctgccgacc cgcaaaccga
     4261 tgaagaaaaa gccgtgcgcg aacgctacga ccgcatcaaa ggcagcgcgg taaaccctgt
     4321 cctgcgtgaa ggcaactccg accgtcgcgc ccccaaagcg gtgaaaaact ttgccaaaaa
     4381 aaatccgcac agcatgggcg catggacaaa agactccaaa acccacgttg ccaccatgca
     4441 aagcggcgac tttttccata acgaacaatc cgttaccgta cctgaagcga cttccgtatc
     4501 catcgtgttc accgacaaac aaggcaataa aaaagagctg cgcgcgcctg ttgccctgaa
     4561 agccggcgaa atcatcgacg cgaccgtgat gagtaaaaaa gccctgctcg ccttccttgc
     4621 cgaacaagta aaagatgcga aagcaaaagg cgtgttgttc tcgctgcaca tgaaagccac
     4681 tatgatgaaa gtgtccgacc cgattatctt cggacacgcc gtcaaagtat tcttcgcgcc
     4741 tgtttttgaa aaattcggcg acaaactggc tgctgccggc gtcaacgtta acaacggctt
     4801 cggcaacctg cttgccaatc tggacaaact ggatgcggac acccgcaccg ccgtcgaagc
     4861 cgaaatcgcc gccgtttacg ccgccaaccc cgatttggcg atggtcgatt ccgataaagg
     4921 cattaccaac ctgcacgttc ccagcgatgt catcgttgat gcttctatgc ctgcgatgat
     4981 tcgtaactcc ggccgtatgt gggataaaga cggcaaagcg caagacacca aagccgtgat
     5041 tcccgacagc agctacgccg gcgtttacca agcaaccatc gacttctgcc gcgaacacgg
     5101 cgcatttgac ccgacaacca tgggtactgt gcccaacgtc ggactgatgg cgcaagcggc
     5161 ggaagaatac ggctcgcaca acaaaacctt tgaaatcgaa gccgacggcc aagtccaagt
     5221 cattgatgcg gcaggaaaag tcctaatgca gcacgacgtt gaagccggcg acatctggcg
     5281 tatgtgccaa accaaagacg ctccggttaa agactgggta caacttgccg tcaaccgcgc
     5341 ccgtctgagc aacacgcctg ccgtgttctg gctcgacgaa aaccgtccgc acgacaagag
     5401 cctgctcgcc aaggttaaag cctaccttgc cgaactggat accaatggcc tcgacatccg
     5461 cgtcctcgct cctgaagaag ccgccaagtt cagcttgggt cgtctgaaaa acggcgaaga
     5521 caccatctcc gtaaccggta atgtcttgcg cgactacctg accgacttgt tcccaatttt
     5581 ggaactcggc acaagcgcga aaatgctgtc tatcgttcca ttgatgaacg gcggcggtat
     5641 gtttgaaacc ggcgcgggcg gttctgcacc gaaacatgtt caacaattcc tcgaagaaaa
     5701 ccatttgcgc tgggactcgc tgggcgaatt cctcgcactc gccgtatcgt ttgaacattt
     5761 ggcgcaaaaa accggcaatg ccaaagccca agtcctcgcc gacactttgg atgcagccac
     5821 cgaaaaactg ctgttgaacg acaaatcgcc taaacgtaaa gccggcgaac tcgacaaccg
     5881 cggcagccat ttctacctca ccctctactg ggcgcaagaa ttggcggcgc aagacaaaga
     5941 tgccgaactg aaagccgcat ttacgccatt ggcagccgct ttgaccgccg acgaagcgaa
     6001 aatcgttgcc gagctttccg ccgtacaagg caaagcggcc gacatcggcg gctactacgc
     6061 cgccaatcct gaaaaagccg cacaagtgat gcgtccgagc gtaaccttta atcaggcact
     6121 aaacgcctta taagcacaaa ggtaaaaatg ccgtctgaac attcgtcgtt cagacggcat
     6181 tagttatccc tatctgcctg attatgatta aacgccgcac ccattggata t
//
LOCUS       DAWKQJ010000115         6207 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000115 DAWKQJ010000000
VERSION     DAWKQJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.115"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..960
                     /locus_tag="WJL20_001550"
     CDS             <1..960
                     /locus_tag="WJL20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7683688.1"
                     /translation="AEPHVPVSIPTATPLPKGEVTLSSDNGKIENINTTSTGSTSVIS
                     IQERAVTKNYFGVESQEKSFIFKTPGGAQYTLSSYADPIVPSYSSPDFKIPDRHAGQR
                     LADGSRIFICCSDSGATREAEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPTSSSY
                     YGSSTPETQGKGKITYQVWGIRVKDGQFVTSSYTPPKGSSFTGYTNTPVLSFITANFN
                     SNKLAGEIRGNSDYGPSVKIENATISGPSFSGNATSGGKTGNLEGKFFGKFNGSYGNT
                     ETSIGGKITFKDDRSLDTVFGGVSYVKKLDETANRDTEHLTKQ"
     gene            988..3414
                     /gene="hupB"
                     /locus_tag="WJL20_001551"
     CDS             988..3414
                     /gene="hupB"
                     /locus_tag="WJL20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ7683689.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFSKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIESRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAKNW
                     ELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPVW
                     QNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALSP
                     WTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
     gene            complement(4002..5636)
                     /gene="groL"
                     /locus_tag="WJL20_001552"
     CDS             complement(4002..5636)
                     /gene="groL"
                     /locus_tag="WJL20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ7683690.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(5729..6019)
                     /locus_tag="WJL20_001553"
     CDS             complement(5729..6019)
                     /locus_tag="WJL20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ7683691.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1511 a         1830 c         1512 g         1354 t
ORIGIN      
        1 gccgaaccgc acgtccccgt gtccatcccc accgccacgc cgctgcccaa aggcgaggta
       61 acgttatcaa gcgataacgg caaaatcgaa aacatcaaca ccaccagcac cggaagcaca
      121 tccgtcattt ccattcaaga gcgggcggtt acaaaaaact atttcggtgt tgaatcacaa
      181 gaaaaatcat tcatcttcaa aacacccggc ggcgcgcaat acaccctttc atcctacgcc
      241 gaccccatcg tccccagcta ctcctccccc gatttcaaaa ttcccgaccg ccacgcaggg
      301 cagcggcttg cagacggcag ccgcatcttt atctgctgca gcgactccgg ggcaaccaga
      361 gaagcggaaa ttaccaaaca agattatatg aaattcgggg catggatagg tcccaacggc
      421 gaaatcgacc tcttcgccgg cggcttcccc gtcggcaaaa caccgacatc aagcagttat
      481 tacggcagca gcacgcccga aacccaaggc aaaggcaaaa tcacttatca ggtttggggc
      541 atccgcgtca aagacgggca atttgttacc tcctcttata cgccgcccaa aggcagctct
      601 tttaccggct acaccaacac ccccgtcctt tcctttatca ccgccaattt caacagcaac
      661 aaactggcag gcgaaatccg cggcaacagc gattacggac cgagtgtgaa aattgaaaac
      721 gcaacgatca gcggtccgag cttttccggc aatgccacct ccggcggcaa aaccggcaac
      781 ttggaaggca agttttttgg aaaattcaac ggcagctacg gcaacactga aaccagcata
      841 ggcggcaaaa tcacttttaa agacgaccgc tccctcgata ccgtattcgg cggtgtgagt
      901 tatgtaaaaa aacttgatga aaccgccaat cgagataccg aacaccttac taaacaataa
      961 accatctcat ccctacaaag gtttcttatg tccatcccct tcaaacccgt attggctgcc
     1021 gccgccatcg cccaagcgtt tcccgccttt gcggcagacc ccgcgccgca gtccgcccaa
     1081 acgctgaacg aaatcaccgt taccggcacg cacaaaaccc aaaaactcgg cgaagaaaaa
     1141 atccgccgca aaaccttaga caagctcttg gtcaacgacg aacacgacct ggtgcgctac
     1201 gaccccggca tttccgtcgt cgaaggcggc agggcgggtt ctaacggctt taccatacgc
     1261 ggcgtggaca aagaccgcgt cgccatcaac gttgacgggc tggcgcaggc ggaaagccgc
     1321 tcttccgaag ccttccaaga attgttcggc gcgtacggca acttcaacgc caaccgcaac
     1381 acttccgagc cggaaaactt ttccgaagta accatcacca aaggcgcgga ctcgctcaaa
     1441 tccggcagcg gcgcattggg cggcgcagtc aattaccaaa ccaaatccgc aagcgattat
     1501 gtttccgaag acaagcccta ccatttgggg ataaagggcg gcagcgtcgg caaaaacagc
     1561 caaaagttca gcagcatcac cgccgccggc aggctctttg gtttggatgc cttattggtt
     1621 tatacccgcc gcttcggcaa agaaaccaaa aaccgctcga ccgagggcga tgtcaaaatt
     1681 gaaaacaagg gatatgtttt cagcccaacc caatcattca gcaagtacca gacctacgta
     1741 gccacagggg ttgcgcgctc ccaacccgac ccgcaagaat gggtaaacaa aagcaccttg
     1801 ttcaagctgg gctacaattt caacgaccgc aaccgcatcg gctggatttt tgaagactcc
     1861 cgcaccgacc gttttaccaa cgagctgtct aatttgtgga cgggtacaat ttactctccc
     1921 gcaacgggcg actaccgcca ccgccaagac gtgagctacc gccgccgctc cggtgtcgaa
     1981 tacaaaaacg aattggaaca cggcccgtgg gacagcctca agctgcgcta cgacaagcag
     2041 cgcatcgata tgaacacttg gacttgggac atcccgaaaa attacgattt gcgcggcatc
     2101 aacggcgagg tttaccattc gttccggcat atccgccaaa acaccgcgca atggactgcc
     2161 gattttgaaa aacaactcga cttttccaaa gccgtttggg cggcgcaata cggcttgggc
     2221 ggcggcagag gggacaatgc caactcggat tacagctatt tcgcaaaact gtacgccccc
     2281 aaaatcctcg cttccaacca agccaaaatc acgatgctga tcgaaagccg gtcgaaatat
     2341 aaatttgcct attggaacaa tgcgtttcac ttaggcggca acgaccgctt ccgcctgaac
     2401 gcgggcatac gctacgacaa aaacagcagc agcgcgaaag acgatccgaa atacaccacc
     2461 gccatccggg ggcagattcc ccatttgggt tcggaacgcg cgcacgcggg cttcagctac
     2521 ggcacggggt tcgactggcg gtttaccaag catctgcact tgttggcaaa atacagcacc
     2581 ggcttccgcg caccgacttc ggacgaaacc tggctgctgt tcccacaccc cgatttctac
     2641 ttgaaagcca acccaaacct gaaagcggaa aaagccaaaa actgggaatt gggtctggcg
     2701 ggcagcggca aagcgggcaa cttcaagctc tcgggcttca aaaccaaata ccgcgacttt
     2761 atcgaattga cgtatatggg cgtttcgtca gacgataaaa acaaccccag atacgccccg
     2821 ctttcagacg gtacggcatt ggtcagctcg cccgtttggc aaaaccaaaa ccgctccgcc
     2881 gcctgggtga aaggcataga gtttaacggc acgtggaacc tcgacagtat cggtttgccc
     2941 aaagggctgc acaccggcct caacgtcagc tacatcaagg gcaaggcaac gcaaaacaac
     3001 ggcaaagaaa cgcccatcaa cgcgctttcg ccgtggacgg cggtttacag cctgggctac
     3061 gacgctcctt ccaaacgttg gggcatcaac gcctacgcca cgcgcaccgc cgccaaaaag
     3121 ccgtccgaca ccgtccacag caacgacgac ttgaacaacc cgtggcctta tgccaaacac
     3181 agcaaggcct atacgctgtt cgacctttcc gcctacctca acatcggcaa acaggttacg
     3241 ctccgcgccg ccgcgtacaa cattaccaac aagcagtact acacttggga atctttacgc
     3301 agcatccgcg agttcggcac ggtcaaccgc gttgacaaca aaacccacgc cggcatccaa
     3361 cgctttacct cgccgggcag gagctacaat ttcaccatcg aagcgaagtt ctaagctcct
     3421 gatgtgccaa tgccgtctga aacccgatga tttgggtttc agacggcatt ttttacggtc
     3481 gcaccgccgc cccctttcag gcttgtacca agcccaatcc cgcaactctc cgtcattccc
     3541 gcaactctcc gtcattccca cggaagtggg aatctagaaa cgcaaagctg caagaattta
     3601 tcggaaatga ctgaaactca acgaacctgg attcccgcct gcgcgggaat gacgaagcta
     3661 tccgcacaga aacctgcacc acgtcattcc cacgaaagtg ggaatccaga acgtaaaatc
     3721 tgaagaaacc gttttatccg atacgtttcc gcaccgacag acctagattc ccgctttcgc
     3781 gggaatgacg gcggaaaggt tgctgttttt ccgataaatt cctgccgctc ttcgtttttg
     3841 ggatggcggg aaataaaaca aaagcgcgcg tatcaaaaaa caacaatgca aagaacggtg
     3901 ttggcaattt taaggtttgc gccttagccg cacttattcg caaaaaaacc gcacggcgtt
     3961 gaccgtgcgg cttgttgtct gaaggcttca gacggcattg cttacatcat gccgcccata
     4021 ccacccatgc cgcccatatc aggcatagcc ggtttgtctt cagggatttc agcaatcatg
     4081 cagtctgtgg tcagcatcag gccggcgata gacgcggcgt gttgcagcgc ggaacgggtt
     4141 actttggcgg ggtcgagtac gcccatttcg atcatgtcgc cgtattcgcc gctgccagcg
     4201 ttgtaaccat agttgccttt gccttccaac actttgttca caaccacgct gggttcgccg
     4261 cctgcgttgg caacgatttg gcgcagcgga gactcaacgg cgcgcaagac gatttgtacg
     4321 cctgcgtctt ggtctgcatt gccggtgtgc aggttttcca gagcggcacg ggcgcgcaac
     4381 agggctacgc cgccgcctgc aaccacgcct tcttcaacgg ctgcgcgggt agcgtgcagc
     4441 gcgtcttcca cgcggtcttt tttctctttc atttccactt cggtcgcggc accgactttg
     4501 attactgcca cgccgcctgc cagtttggca acgcgctctt gcagtttttc tttgtcgtaa
     4561 tcgctggttg cggtttcgat ttgttggcgg atttcggcaa cacgcgcttc gatttgggct
     4621 gcgtcgccga agccgtcgat gatggtggtg ttttctttac cgatttcgat gcgtttggcc
     4681 tgacccaagt cgtccaaagt cgctttttcc aaagacaggc cgacttcttc ggaaatcact
     4741 gtgccgccgg tcaggatagc gatgtcttgc agcatcgctt tgcggcggtc gccgaagccc
     4801 ggagctttaa cggcaacggt tttcagaatg ccgcggatgt tgttcacgac caaagtcgcc
     4861 aaggcttcgc cttctacgtc ttcagcgata atcaacagcg gacggctggc tttggccact
     4921 tgttccaaaa caggcagcag gtcgcggata ttgctgattt ttttgtcgaa caacaataca
     4981 aacggattgt ccaagccggc gatttgtttt tccgcatcgt tgatgaagta aggagacagg
     5041 tagccgcggt cgaattgcat accttcaacc acgtccagct cgttttccaa agatttgccg
     5101 tcttcaacgg taatcacgcc ttctttgccg actttttcca tcgcttcggc aataatcgcg
     5161 ccgacttgtt cgtcagagtt ggcggaaata gagccgactt gggcgatttc tttagaagtg
     5221 tcgcaaggtt tggcgatgtt tttcagctct tcaaccaaag cggcgacggc tttgtcgata
     5281 ccgcgtttca ggtcggtcgg gttcataccg gcggtaacgt atttcatacc ttcggcaacg
     5341 atggattgcg ccaatacggt ggcggtagtc gtaccgtcgc ccgccacgtc gttggttttg
     5401 gacgcgactt ctttcaccat ttgcgcgccc atattttcaa acttgtcttt cagttcgatt
     5461 tctttggcga cggttacgcc gtctttggtg atgtgcgggc cgccgaaagc gcggtcaacc
     5521 accacgttgc ggcctttggg acccaaggtt acgcgcacgg cgtttgccaa aatgttcacg
     5581 ccgtttacca ttttttggcg gacttcattg ccgaattgta cgtcttttgc tgccatttca
     5641 attctccaaa aatcattaaa actgtctgat aaaaccgttt atgccgtctg aaggcggttt
     5701 gccgtttcag acggcatcgt gtccgtattt atttttcaac gatgccgaaa atatcttctt
     5761 cgcgcattac caacagctct tcgccgtcgg cttttacggt ttggccgctg tatttgccga
     5821 agatgatttt gtcgccgact ttgacatcca gcggacggcg cgcgccgtct ttaccgattt
     5881 tgcccgcgcc cacggcgatg acttcgccca tgtcgggttt ttcggcggcc gcacccggca
     5941 aaacgatgcc cgatgcggtt ttttcttcag cttccaagcg tttgacgaca acgcggtcgt
     6001 gtaaaggacg gatggtcata tttatgctcc gataaaatga tttgaaaaca atcatctgcc
     6061 cgaacggttc ggacagattg aagtggaaac cgggctgccg cctgccggcc cgcccgtata
     6121 agtcggcaaa attagggtgt gtgcgggtaa attcaagtga ggcggaaaaa aatttatttc
     6181 cgccgttttt tatagtggac taaattt
//
LOCUS       DAWKQJ010000116         6091 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000116 DAWKQJ010000000
VERSION     DAWKQJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.116"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..2074)
                     /locus_tag="WJL20_001554"
     CDS             complement(68..2074)
                     /locus_tag="WJL20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ7683692.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2363..4390
                     /locus_tag="WJL20_001555"
     CDS             2363..4390
                     /locus_tag="WJL20_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ7683693.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4482..6035)
                     /locus_tag="WJL20_001556"
     CDS             complement(4482..6035)
                     /locus_tag="WJL20_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ7683694.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1253 a         1421 c         1861 g         1556 t
ORIGIN      
        1 cttttatggg gatttcccgc atcagggcgg tattcggggc gaatcccgct tcagacggca
       61 ttgccggtca aggcttgtcc gaaggaggat gtccctgttc tttggcgggc gggaggaaaa
      121 gtatgcaggg attgctttta atctgattcc aaaaactctt aatcagattg cggatgctgc
      181 cgattttcgt gccgcgcgag cgcagggcga cgaagagggc gaggctgaag ctgacgcaga
      241 ggttgaccag gccgatggcg aggacgctga aaatgccgag tacgaacgtg cccaaaccga
      301 cgttgccgct gacggcggca tagccgaggt tggcggagga aaaggcgacg tggcggatgt
      361 ccagcggcag cccgaggagg tgtccgaaat agccggtcat acccaagagc atcccgaaga
      421 tgaagttgcc tgccaacgag ccgtagtgtt tgtggatgta ggcggcgagg acgcggcggg
      481 gcccggggcg catgatttta cgcagcaagg ggttgaaggg caggcgttgg cgcaggttga
      541 ggtagtcggc gcggttgtcg aaaaaacctg cgatgatgcc ggagcagaac agccacagac
      601 ctgcaatggc ggcataccac agcgtcgggt aagcgatgat gtctatggat ttgaactggt
      661 aggcggcggt gtgggcatcg agtatgggct gccggtacag atgggcatag ccgaacgata
      721 tggcgcacgc caaaaggatg gcgatggaaa cgttgccgaa aacggcgaca ctttgggaac
      781 ggcatacgtc gatgaggagc ttggcgagtt tgttgtccac cgctttgccg ccttcgttga
      841 gatcgacctg ttcggcaaag ctggcggcag tcatcgcggg ctgtttggtg gcgacggtgc
      901 agtgcagcat atggatgatc ataaagccga tgccgtagtt gaacccagcc gacaaggaag
      961 tcaggaaggg gctgagtccg agtgtgccga tgcggatttt gagcagcgcc atcagggcga
     1021 tgatgatgcc gccgcctgcc gccgagtaga acattttgaa atattctttg cggttgcggg
     1081 tgatgtagtg ttcgccgtgg tggcttttgt tttcggtaat gctgcgggcg agcatacgga
     1141 tgctgctgcg gcgcaggtgg cgggtgctgt attgttcgac cgccgcgtag atgagggagt
     1201 tcatcagcgc gatggtcagg cggttgctgt tgccggcgcc ggtttggatg tcggtgagca
     1261 gcttcagacg gtctacggtc tgccggagcc gttcgagcag gtgggcgact ttgaccgacg
     1321 aaccggagcc ggcgcccgtc cctttgcggc gcaaatagtc aatctggctg aaacattggt
     1381 cgaacatcac ttcgaggtgg gcggtgtcgt aaggcgtggt gccgttgcgg tagtgttcga
     1441 ccagtttggc ggtttcgcgt tggagggcga cgaaggaaga atcggcttcc agcaggcgcg
     1501 gggcgatgcg gatgaggtcg ggttcgatgg cttcggatgc cgtccagaca gacagcattt
     1561 cgatggcgcg caaacgcgca tcggcaaggc ggatgcccgc cgtctgcaat ttggacggct
     1621 cggcgtggct ccggatgagt tcgtataaaa ccagccattg gcggatattg agcgtttgca
     1681 gccatttgtc gtcgttttcg gaatggaaaa gataaaggaa gacttcgccc aagttggcaa
     1741 aatctttata tgacgggctg aagcgttcgt agatgcgtat gcccatttcc cgggcaaagc
     1801 tgtggcgcga gaagatgccg agtttgatga gtgccggata aatgtgtatt tgcgcgagcc
     1861 agatgtaaaa acaccggctg aagcgggaca gtaattccct gtcttgtttg aatgtgtcga
     1921 taatcaggtc gaaacgttcc cccgcacatt ttttgccgcc acggcgcaaa aatttaatca
     1981 gtgcgttgag gacgttgaca aaatcggtat gtcccaagct ttccgaaagc agggggcgta
     2041 ggttttgagg ggtaattttc ttcatatcag ccattgtacc gcatcggcaa tccggcgtta
     2101 aacgccccga tggcggggag ggcgcgcctg cggtgggcgg cgcattttcc gccggtttga
     2161 aatcaaacta tattaatgat ataataaata tcgataaatc aatctgatag aataatccgt
     2221 attgattttt aattttttcg attataacaa tttcggccgg tttgtgcatt tcgtcatccg
     2281 gaagccggta ttttaccggc aggttttgac cgctttgccc gatgaccggg tttgtctgtt
     2341 tttaacaaag gagtgagaag gtttgtccct gtctgaattt atagaacgcc gaacgtcatt
     2401 taatctgatg gttattttga cgactttgtt ttttgtgtgt gttttggtgg tattggtttt
     2461 aaccgtgccg gatcaggtgc agatgtggct cgatcgggca aaagaaatca tttttaccga
     2521 gttcagctgg ttttatgttt taacgttttc catttttctg ggtttcctgc tgatactctc
     2581 ggtcagcagt ttgggaaaca tcaggctcgg acgggatgaa gatgtgccgg aattcggctt
     2641 cctgtcgtgg ctggcgatgc tgtttgcggc cgggatgggc gtgggtctga tgtttttcgg
     2701 cgtggcagag ccgttgatgc attatttttc ggacattacg gccggcacgc cggaacacag
     2761 gcagcagcag gcattgctgc acacggtgtt ccattggggc gttcacgctt ggtcggtgta
     2821 cggtacgatt gcattggctt tggcttattt cggtttccgc tacaagctgc cgcttgccct
     2881 gcgttcttgt ttttaccccc tgttgaaaga aaaaatttcc ggaaggttcg gcgatgccat
     2941 tgatattatg gcgttgcttg ctactttttt cggcatcatc accacattgg ggttcggggc
     3001 ttcgcaactg ggcgccggat tgcaggaaat gggctggatt gccgaaaaca gcttcagcgt
     3061 gcaggttttg attatcgccg ccgtcatgtc cctcgccgtc gtttcggcaa tatccggcgt
     3121 ggggaagggc gtgaaggtgt tgagcgagtt gaacctgagc cttgcgtttt tgctgctgtt
     3181 ttttgttttg gcggcgggtc ccactgttta cctgttgtcg gcattcggcg acaacatagg
     3241 gaactacctc ggaaatctgg tgcgcctcag ttttaaaact tatgcgtacg aacgggaaca
     3301 caagccgtgg tttgaatctt ggacggtgct ttattgggcg tggtggtgtt cttgggcgcc
     3361 gtttgtgggt ttgtttatcg cgcgcatttc aaaggggcgc accatccgcg agtttgtctt
     3421 cggggttttg ctcatccccg gcctgttcgg cgttttgtgg tttaccgtct tcggcaatac
     3481 ggcgatttgg ctgaatgacg gggttgcggg gggaatgctc gaaaagatga cctcctctcc
     3541 ggaaacgctg ctttttaaat tctttaatta cctccccctg cccgaattga cgagcatcgt
     3601 cagcctgctg gtcatttctc tgttttttgt aacttctgcc gattccggga tttatgtcct
     3661 gaacaatatt acctctcggg acaaaggctt gagcgcgcca cggtggcagg cggttatgtg
     3721 gggcgtgctg atgtctgccg ttgccgtttt gctgatgcgc tcgggcggac tcggcaacct
     3781 gcagtctatg accctgattg tttccctgcc gtttgccctg ctgatgctga taatgtgttt
     3841 cagcctgtgg aaaggcttga gtgcggataa gaaatatttt gagacccggg ttaaccctac
     3901 cagtgtattt tggacgggcg gcaagtggaa agaacggctg gtgcagataa tgagccagac
     3961 gcaggagcag gatattttaa aattcctcaa acataccgca tcgcccgcta tgcacgagtt
     4021 gcaacgggag ctttcggaag aatacggctt gagcgtccgg gtcgataaga tgtttcatca
     4081 ggacgagccc gcaatcgagt tcgtcattcg gaaagagacg atgcgcgatt ttatgtacgg
     4141 gattaagtct gtcgggcagg atgtatccga ccagttgatt aacgacggca agctgccgca
     4201 tatccggcat cagacaactt acaaacccta cgcttatttt ttcgacgggc gcgtcgggta
     4261 cgatgtgcag tatatgaaca aggacgagct gattgccgac attttgaaaa actacgaacg
     4321 ttatttgatg ttgttggatg atgtcggtca ggaactgatg gcgcacgagc aggtggaatt
     4381 ggcagagtaa gtgccttatc tctggcgttt gccaaaaaaa atgcggtgaa tcagattcac
     4441 cgcatttttt ttgccggacg gggcttcgga cggcacggcg ttcatttgtt tccgtcaaac
     4501 tgctccttca acacccgttt caataccttg cccgtagcgt tgcgcggcag cccgtcttta
     4561 aagtggattt gtttggggat tttgaaattt gccagcacgg tacgaaggtg gcggcggatt
     4621 tcgttctcgc ccaaatccat accttccttc aattggacga aggcgacgat ttcctcgtcg
     4681 gcataacggt ctttcacgcc gatgacggcg gcggcttcga cggcatcgag tttgtagatt
     4741 tcttcttcga tttcgcgcgg atagacgttt tgacctttgg aaataatcaa atcttttttg
     4801 cggtcgacga taaagataaa gccgtcttcg tctatggtaa cgaaatcgcc cgttttcaac
     4861 cagccgttga cgatggtttc atcggtggcg gcaggcatat tgaggtagcc ccgcatcacc
     4921 gaaccgcccc tgacgatcag ttcgcccact tcgccgcgcg gcacttcgac cagttcttca
     4981 tcgacggctt tgacttccaa accgggcagg gggatgccga cgctgcgggc tttttgcctc
     5041 tcgggcgtat tgacggcgac gacgggtgag gcttcgctca gtccgtagcc ttccagcaat
     5101 ttggcgcggg ggaacttggc tttgaaatcg aggatggttt gttccgccaa aggcgcaccg
     5161 ccgctgataa acaggcggat gcggttgaac catctgaaat accaagggat tttcgccttg
     5221 ctcatcgcgg tgtaaatcgc gggtacgccc aaaaacacgg tcgcgcgttt gagcagggcc
     5281 tgtttcaaaa cgttggaaaa cgggaaaacg gatttgacca aaataatcga acacgccata
     5341 taaatcggca gcagcaccat agccgtcagc gtgaagctgt ggaacatcgg caggaaaacg
     5401 ataaagcggt cgcgcttgga gattttaaag atgcgttcga tgccgttcag gttggcgaac
     5461 aggttggcgt aactgattag cgcgcctttg ggatgccccg tcgtgccgga ggtgtagatg
     5521 atgtgtgcca aatcatttat ccggggttgt cggcccaagt cgggtttttc ggggaagcgg
     5581 cgcacgtctt caaaaaaggc atcgccttcc gccatttcgc cggtcggacg gcttttgtcc
     5641 gtccaaatga ttttttcgac gggcgtttgc gccttcaagc ccgccaattc ttttgacagg
     5701 ccggccgagg cgaacaggaa gcgcgccttg cagtcgttca ggatatacgc gtattcgctg
     5761 tttttcaaaa atgtgttcat cggtacggcg accgcgccga tggcggagat ggcgaaatag
     5821 gcggtaataa attctgtgga attggaaacc gccagcgcga ccgtgtcgcc gaacttcacg
     5881 ccgatatttt gcagatacgc cgcaacggct tcggcctcct gcttgagcgc gcggtaggcg
     5941 gttttttcct tgccgtcgaa cactgccgtg ccgttgccgt ttttgcggca ggcggcggcg
     6001 agcattccgt agaaattggc ataagtccgg ttcatgaaac ttcctttatg gttgcggacg
     6061 gcgaacgccc tcatttgaaa acaatatgcc g
//
LOCUS       DAWKQJ010000117         6070 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000117 DAWKQJ010000000
VERSION     DAWKQJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.117"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(303..2339)
                     /locus_tag="WJL20_001557"
     CDS             complement(303..2339)
                     /locus_tag="WJL20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ7683695.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVTDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPKDSLAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2427..3299)
                     /locus_tag="WJL20_001558"
     CDS             complement(2427..3299)
                     /locus_tag="WJL20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7683696.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3569..5959
                     /gene="gyrB"
                     /locus_tag="WJL20_001559"
     CDS             3569..5959
                     /gene="gyrB"
                     /locus_tag="WJL20_001559"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ7683697.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         1385 a         1621 c         1738 g         1326 t
ORIGIN      
        1 cctgattttt gttaatccac tataaaatgc cgtctgaagc gttgtttgac cgtttcagac
       61 ggcattttta tcaaattcaa aaatattccg tcattcccgc gaaagcggga atctagaacg
      121 taaaatctaa agaaaccgtt ttatccgata agtttccgca ccgacagacc tggattcccg
      181 cctgcgcggg aatgacggga ttttggctta ctgtttttgt gaaaatggcg aaaccgtgca
      241 aatgctgatg ccgtctgaaa cactgtttga ttgtttcaga cggcattttt tgttcacatc
      301 gatcaagccg cgttgtcgaa accgctgtgg cgcaagagtg cgtctatgcg cggttcgcgt
      361 ccgcggaagg ctttgaagga ttctgccgcg ctgcgcgatc cgccgacggc gaggatttcc
      421 tgccaaaagc gtttgcctgt ggcggcgaca tcgtcgcttt cttcaaaggc ggcgtatgcg
      481 tccgcgctca atacttccgc ccacgcgtag ctgtaatagc ctgcggaata gccgcctgcg
      541 aagatgtggc cgaagctcaa ggcgaagcgg ttgtattcgg gcggttggat gacggcgact
      601 tctttgcgca cgctgtctaa aacctgttgc cagtttttca gacggccttc gtcgtcttcg
      661 ctgtaaatca tcatatcaaa gagggcgaac tccatttgcc ggacgaggaa catgccgcgt
      721 tggaagtttt tggcggcgag cattttgtcg aagagttctt tcggcagggg aacgccggtt
      781 tcttcgtggg ctgacatttg tgccaagaca ttgtattccc aaacgaaatt ttccataaac
      841 tggctgggca gttcgaccgc gtcccattct acgccgttga tgccggatac gcccagttcg
      901 tccacttggg taagcaggtg gtgcagcccg tgtccggttt cgtggaagag gatgaggatt
      961 tcgtcgtggc tcaggcgggc ttccctgccg ccgacgggtg gggcgaagtt gcagacgagg
     1021 taggcggtgg gcagttgcag cgtgccgtct gaaaaacggc ggcggccttt gtagtcgttc
     1081 atccacgcgc cgccgcgctt gccttcgcgg gcgtagaggt ccatatagac gccgcctatg
     1141 gttttgccgt tttgttgcaa ttcaaaatag cgcacgtctt tgtgccagac ggggacggtt
     1201 ttttcggtaa atccgatgcc gtagagtttt ttgatttggg cgaacagtcc gtttaatact
     1261 ttgccgacgg ggaagtattt tttgacttcg gtttcgctga acgcgtattt ggcttcgcgc
     1321 agtttttcgc tggcgtagcc caagtcccac ggttgcaaat cggcgaggcc gaggctttcg
     1381 cgggcgaagg ctttgacttc ggcgaggtct ttttcggcgt agggtttggc gcggcgggcg
     1441 aggtcgtgca ggaagtttaa aacttgttcg ggggtgtccg ccattttggt tgccagcgac
     1501 aattcggcgt agtttttgaa gccgagcagt ttggcggttt gcagggcgtt tgcgagcgtg
     1561 cggtcaatgt tggcggtgtt gtcgaatttg ccgccgtctg acagttcgct ggcgcgggta
     1621 acgtaggcgc ggtagatttg ttcgcgcagt tcgcggttgt cggcgtattg gatgacggcg
     1681 aggtagtgcg gaatctgcaa accgattttg tagcctgttt tgccttcgct ttgcgcggca
     1741 gcggcaaaca tggcgagcga gtctttggga atgccggcaa gcggtgcggc atcgtcaaag
     1801 taaatgccga acgcgtcggt cgcgtctagg acgttttggg agaatttggc ggaaagttgc
     1861 gcgccttcgg tttgcagttt tgccagttct gcctgctgtt cgggcggcag ttccgcgccg
     1921 ctaaggacga aatcgcgcag gtcgtggttg agcttggttt tttgtgcggg ggagagggtg
     1981 tcgaattcgg gggaattttt gatggttttg aagcggttgt acagctcgat gtcttgtccg
     2041 atttcggtga agaagacggt gatttcgggc atcagttcgt tatagacggc gcgcagttcg
     2101 ggcgtatcgg tgacggagtt gaggtgcgac accacgcccc aaatcctgcc gacgcgttcg
     2161 gtgatgccgg tcaggggttc gacagtgttt gcccagccgg tgtgcgtttg ggctttgatg
     2221 gcggcgattt gttcgcgcgc ttcggcgatg gcggtttgca gggcgggttt gatgtcttcg
     2281 gttttgattt gatcaaaacg gggttcttcg cccaaatgga gcagtgcgtt gtcagtcata
     2341 agatgggttt cctttgcgtg ttgtgcttca gacagcatat cggcaatatg ccgtctgaag
     2401 ccggaaatgg ggtcggtcgg ggaaagttaa agtgtttcgg agccggcggg gtaatttaac
     2461 aggcggtatt gtacgcgcaa aaattcgtcc ataaccgcct gttggatttc cagccgcttt
     2521 gaaacggggg aggcgaagcg gacgatgatg cggtatgcct tgtcatcgta cggcacgcgg
     2581 gtaacgcgcg gtctggcggc gggcgtgata aacagttttt ccgcctgcac gttttccaaa
     2641 tgccgttgga tggcggggat gtagggcgcg cacaagggct cgagtacggc tttcagacgg
     2701 catacggctt catccgaatc caaatggatg gggacgggga tttcgaccgt atggatgaca
     2761 tagtcgccca aaatattgtc gcggcgcacg gggtggctca acaacaggct gttggggaaa
     2821 gaaacggtgg ttcccgcaag ctgtccgacc aaggggttcg gaccgacctg catcatcagc
     2881 gtgttcaaca ggttgatgtc gaccacgcgc ccgcgcaggc cgttgatttc gatatagtcg
     2941 ccgaccgagt attgctgggt ggcagacctt aaaatactgc ccgacagaca cataatcagt
     3001 tccttcgtcg ccacgacgac cgccgccgcc accgcaaaca tcgacaaagc cagcgtttgg
     3061 atttgcgccg accagataaa tgccagcgaa aacagcacca aaagcagcgt tatattgcgg
     3121 ctggcaacca aaaaccgccg cttgctttcg atgccgaaat ccggatgccg tttgaagtgg
     3181 atattcaaca gaagggcgcg cgccagcagc aaagccgcaa ccgccgccac ggattcgacc
     3241 gcctccgcac gtatcgggac ggcaccgagc caagtgtcca acctattcca tatttccatt
     3301 tccgcgcccc tgtccgaaaa gtatagggtg tagattttag tggcaaaaaa acgcttttgc
     3361 cactttacgg gaaaatcccc gacctgatgc cggaggcaaa cgtaccgttt catcttttcc
     3421 cggcggcaca ctgatgcgga taacgctgaa ttaggcggca aaatcggcta taatacacaa
     3481 ttatatatgc cgtcccgccc gctttttcca caaggtgcgg acttgttttg gcggcatcgc
     3541 aaaatctttt caaaatccgg caaaaaatat gactgaacaa aaacacgaag aatacggcgc
     3601 cgacagcatt caggtgctcg aaggcttgga agcggtacgc aaacgccccg gcatgtacat
     3661 cggcgacacg caggacggca gcggcctgca ccatatggtg ttcgaagtat tggacaacgc
     3721 cattgacgaa gcactcgccg gacattgcga caaaatcacg gtaacgatac acgccgacca
     3781 ttccgtcagc gtcgccgaca acgggcgcgg tatgcccacc ggcatccacc cgaaagaagg
     3841 acgctccgcc gccgaagtca tcatgaccgt attgcacgcg ggcggtaaat tcgacaacaa
     3901 cagctacaaa atctccggcg gcctgcacgg cgtgggcgtg tccgtcgtca acgcgctgtc
     3961 cgactgggta acgctgacca tctaccgcga cggcaaagaa cacttcgtcc gcttcgtgcg
     4021 cggcgaaacc gaagagccgc tgaacgttgt cggcgattcc gataaaaaag gcacgaccgt
     4081 gcgcttcctc gccagtacgg aaaccttcgg caacgtcgaa tacagcttcg acatccttgc
     4141 caaacgcatc cgcgaacttt cgttcctgaa caacggcgtg gacatcgaat tgaccgacga
     4201 gcgcgacggc aaacacgaaa gcttcgccct ttccggcggc gtggctggat tcgtgcaata
     4261 catgaaccgc aaaaaaacgc ccttgcacga aaaaatcttc tacgcgttcg gcgagaaaga
     4321 cggcatgagc gtcgaatgcg caatgcaatg gaacgacagc tatcaggaaa gcgtgcagtg
     4381 cttcaccaac aacatccctc agcgcgacgg cggtacgcac ctgaccgcgt tgcgccaagt
     4441 gatgacgcgc accatcaaca gctacatcga agccaacgaa gtcgccaaaa aagccaaagt
     4501 ggaaaccgcc ggcgacgata tgcgcgaagg tttgacctgc gtgttgtccg tcaaactgcc
     4561 cgaccccaaa ttttcgtccc aaaccaaaga caaactggtt tccggcgaaa tcggtcccgt
     4621 cgtcaacgaa gtcatcagcc aagccctgac cgacttcctc gaagaaaacc ctgccgaagc
     4681 caaaatcatc accggcaaaa tcgtcgatgc cgcccgcgcg cgcgaagccg cccgcaaagc
     4741 ccgcgaaatc acccgccgca aaggcgtgat ggacggtttg ggcctgccgg gcaaactcgc
     4801 cgactgccaa gaaaaagacc ccgcattgtc agaactttat ctggtcgagg gcgattctgc
     4861 aggcggttcc gccatgcagg ggcgcgaccg caaattccaa gcgattttgc cgcttaaggg
     4921 caagattttg aacgttgaaa aggcgcgctt tgaaaaaatg ctcgccagtc aggaagtagc
     4981 cgctttgatt accgcgttgg gcgcgggcat cggcaaggaa gaattcaatg ccgagaagct
     5041 gcgttaccac cgcatcatta tcatgaccga tgccgacgtg gacggcgcac acatccgcac
     5101 cctacttttg accttcttct accgccaaat gcccgaactg gtcgagcgcg gctacatcta
     5161 tatcgcccag ccgcctttgt ataaagcgaa atacggcaaa caggagcgtt acctcaaaga
     5221 cgaactggaa aaagaccaat ggctgctcgg tttagccttg gaaaaagcca aaatcgtttc
     5281 agacagccgc accatcgaag gcgaagagct tgccaatacc gccaaacaat tcctgttggc
     5341 caaaaccgtc atcgaacagg aaagccgctt cgtagacgaa ctcgtcctgc gcgccatgct
     5401 acacgcgtcg cccattgatt tgacgtcgtc tgaaaacgcc gataaagccg ttgccgaact
     5461 ttccggtctg cttgacgaaa aagaagtcgc cctcgaacgc atcgaaggtc atgaaggaca
     5521 ccgattcatc aaaatcacgc gcaagctgca cggcaacgtc atggtcagct acatcgaacc
     5581 caagttcctc aacagcaaag cctaccaaac cctcacccaa accgccgccg cgctcaaagg
     5641 catggtcggc gagggtgcca agctttacaa aggcgaaaac gggtacgacg cggacagctt
     5701 tgaaaccgct ttggacatct tgatgagcgt tgcccaaaaa ggtatgtcca tccaacgata
     5761 caaaggcttg ggcgagatga accccgaaca gctgtgggaa accacgatgg atcccgccgt
     5821 gcgccgcctg ttgaaagtgc gcatcgaaga cgccatcgcc gccgacgaag tgttcgttac
     5881 gctgatgggc gacgaggtcg agccgcgccg tgcctttatc gaaaacaacg cgctgattgc
     5941 ccaaaatatc gacgcataag tgccgtttta aaaaaggaga cgggcatcgt gccgcgtctc
     6001 cttttggttt gtcaaacgga agccatgccg tctgaaaccg cctttcggag caaaaccatg
     6061 atcagcattt
//
LOCUS       DAWKQJ010000118         6019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000118 DAWKQJ010000000
VERSION     DAWKQJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.118"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..1456)
                     /gene="mtrE"
                     /locus_tag="WJL20_001560"
     CDS             complement(53..1456)
                     /gene="mtrE"
                     /locus_tag="WJL20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ7683698.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1511..4714)
                     /gene="mtrD"
                     /locus_tag="WJL20_001561"
     CDS             complement(1511..4714)
                     /gene="mtrD"
                     /locus_tag="WJL20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ7683699.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4726..5964)
                     /gene="mtrC"
                     /locus_tag="WJL20_001562"
     CDS             complement(4726..5964)
                     /gene="mtrC"
                     /locus_tag="WJL20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ7683700.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIVGITGAKKVTPKEWAPSENQA
                     ASPQSGVQTASEAKPASEAK"
BASE COUNT         1355 a         1701 c         1616 g         1347 t
ORIGIN      
        1 aaatgccgtc tgaaacctta tcgtttcaga cggcatttta ttgcccgacc ggttatttgt
       61 cggtttgggt atcccgtttc aatccgccgc cgagtgcctt gtacaaatcg gcaaggtttt
      121 cggcgcgggt cagttgtgcc gacaaagccg caccttccgc cgaatagctg ctgcgttccg
      181 catcgagcaa gtcgagcgcg ccggatacgc cgtgtttgta acgcagaccg accaaacgca
      241 acgcttcttt agaggcgcgg ctttgtttgc ttaaagcgtc ataggcttta tccagctgct
      301 cgcgcgccgc caatgcgttt gccacgtctt gaaaggcgga ttggacggcg gcttcatagg
      361 caacgatttg tgcctgttgg cgcagcttgg ctacatcaag gttggctttg ttcgtacccc
      421 aggtaaaaat cggcagggta atagacggcg caaacgacca aacgcccgtg ccgcttttga
      481 acagcccgcc caattcggca gaatgcgtat cgacgcttcc ggtcaggcgg atggatggga
      541 aaaaggcggc gcgtgccgca ccgatattgg cgtttgcctg tttgagcgcg tgttcggcag
      601 cacggatatc gggacggtcg agcaatactt cggaactcaa accggccggc agcttctcaa
      661 caaaaaactg cttgtccaac ggcaaaccgg cgggcaggtc gtcgggtatc ggttggttaa
      721 tcagggttgc caaggcattg cgcgcctgtt cgcggctgcg cgcggcatgg gcataatcgg
      781 ctttggcaga ttcgattagg gcttcctgct gacgtagggc gacggcggaa atcacacctg
      841 ccttgtaacg taattcggac agcttgtagg tttcctcgcg cgttttcaaa acacgttgcg
      901 ccaaagacat cgcttcttcg gcataacgtt cgttgaaata ggctttggca acggtggcaa
      961 tcaggctcaa atgtgccgca tcgcggttgg cggtgctggc gaaatagcct tgcagtgccg
     1021 cctcgctgct gctgcgtaca cgcccgaaca gatcgagttc gtaagatgcc gcacccagtc
     1081 cgactttgta gctgctgctt acattgccgc cgctcaagct gccttggcgc gagtcgttcg
     1141 cattggcggc aagcgtgggc aggaggttgt tgcgctcaat catgtattgt ttgcggtaga
     1201 tttcgctgtt caatacggcg gtacgcaaac tggtattgcg ctcgagtgcg atgtcgatca
     1261 gcttttgcag gcgcgggtcg gcaaaatagt cgtgccaacc caaatcgacg gcgcggatgc
     1321 cgctgtcggc ggtatcgttt ttgaacgttt cggcaacttc gactttgggc tgctcgtatt
     1381 ggggaatcat ggtgcaggcg gataatgcga aggctgctgc aacagaagtc aaggtggttt
     1441 tcaatgtagt atccataaaa aagtcctgat gccgtctgaa aacccgtggg cgttcagacg
     1501 gcatggttgc ttaatgttgg ctgtcgtccg aaccggtgat gcccgcttcg gcagcgtgtt
     1561 ttactgccat ttcgtgttcg tgcgcggttt ctttgaagaa tttgcgcacc accacataga
     1621 aaagcggaac aaggaacacc gacaagagcg tgccgatgag catcccccag aatacggttg
     1681 taccaatggc gcgctggctg gcagaacttg caccgccggc aatatacagg ggaaccacgc
     1741 ccaaaataaa ggcaaacgag gtcatgataa tcggacggaa acgcaggcgg gctgcttcca
     1801 aagcggcttc aaccgcgctt ttcccttgcg cttgaaggtc tttggcaaac tcgataatca
     1861 aaatcgcgtt tttcgcactc aaacccatca cggtaacgaa accgacttga aagtagatgt
     1921 cgttggcgaa cgagggaacg ctgcccaaca gtccttcaaa caggttgcgc ccggttacgc
     1981 ccgcagccgc accgatcaaa cccaacggaa tcacaaggat gaccgccagc ggaatcgacc
     2041 agctttcata aagcgcggca agtaccaaaa atacggctgc aaccgccaaa ccgtacaaaa
     2101 tcagggtttg cgagccgcct tttgcctctt cgcgcgactg tccgccccat tcgaagctgt
     2161 aaccgccgcc caattcgtca accatttttt gaaccgccgc catagcctgc ccggtggaaa
     2221 cgccggttgc aggcgaagcg gacagcttca tcgaaggata accgttgaag cgtacgctct
     2281 gttccgtacc gttttcccaa gaaacagtag caatggtgga aagcggtacg gcgacgccgg
     2341 atttgttcgg cacggtcagg ttcaaaatat cggcaggctg catacgggca tcctcgtcgg
     2401 cctgcaccat cacgcgttgc agacggcctt ggttcgggaa gtcgctgaca taagacgaac
     2461 tcagcgcgct tgccaatgcg gtgcggatgt cggcaaacga aatgccttgc gccgccgcgg
     2521 cggcacggtt gatgtcgatt ttcaactgcg gcgagtcttc caaaccgcca gcacggacgg
     2581 tgctggggtc aaacaaaccg ctggcacgca ttttctgaat caactcgttg cgcttcgcca
     2641 gcaatgcggt atggccggta ttattgcggt cttgcaggtt gatgctcagg cccgaaccat
     2701 tgcccaactc cagaatcgga ggcgggacga cggcgatgcc aaaaccgtct ttaagcgtcc
     2761 ccatcatcat acccgtcagc ttgccggcaa tcgcaacggc atcgctgccg ggcgcggtac
     2821 gctcgttcca atctttcaat atggcaaaac ccatcgccat attctgaccg ctgcccgaaa
     2881 agctgaagcc ggaaacggta atgatgtttt ctatttcagg aatgcttttc gccagttggg
     2941 taacttgcgc caaagtcgca ttggtgcgct cttgggtcgc tcctgcaggc agttgcacgc
     3001 tgaccatgac gaagccttgg tcttcggtcg gcaggaatga agtcggcagg cgcataaaca
     3061 ggaacacgcc cacaaccgcc aagccgatat agacaaccat catgcggaaa gtcttacgca
     3121 gcactttggc aacccggcct tcgtaaccgt gcgtccaact gttgaatttc ttgttaaacc
     3181 agccgaagaa accttttttc tcttcgtgat gccctttcgg gattgtcttc aacattgtgg
     3241 cacacaaagc aggggtaagg gtcagcgcaa ggaaggcgga gaatgcaatt gatgacgcca
     3301 tcgtcagggc aaactgtttg taaatattgc ccgtcgcccc gctgaacatc gccaacggta
     3361 cgaacacgga aatcagaacg gcggtaatac cgatgaccgc gcccgaaatc tgacccatcg
     3421 cttttttggt cgcttctttg ggcggcaagc cttcacccgc cataatgcgc tcgacgtttt
     3481 caaccaccac aatcgcgtca tcgaccacga tgccgatgac caaaaccatc gcaaacatgg
     3541 tcagtacgtt aatcgacatg cccatataag agatgaaggc gaaaccgccc aacagcgaaa
     3601 tcggtacgac gatggtcgga atcagcgtat aacggatgtt ttgcaggaag agatacatta
     3661 cgacaaacac caacaccatc gcttcgatta aagtgtgaat cactttttca atcgaaattt
     3721 cgacgaattt ggaagtatcg taaggggttt tccagctcat accctgagga aagtattttt
     3781 ccaacgtcgc catgcgttct ttaaccgcct ttgccgtcgc catcgcattg ccgctgttgg
     3841 acagcatcac cgccataccg gtggtattta caccgttcag acgggttgag gaagaatagt
     3901 cttccatacc cagtccgacc cttgccacat ccttcaggta aacattagaa ccgtcggtat
     3961 tggcgcggag gatgacgttg ccgaattctt ctgccgtacc caactgccct tgcgccgtta
     4021 cggtagccgt aaccgtctgt ccgcgaacgg cgggaagcga accgatagaa cccgctgaaa
     4081 tctggacgtt ctgggcggac agcgcgctgc cgatatcggc aaacgacaaa ttgtagtttt
     4141 gcagtttctt aggatcaacc caaatccgca tcgcgcgttg cgcgccgaac aggcgtacct
     4201 gccccacgcc ttcgatacgc tgcaactcgg gaacgatatt acgctgcgcg tagtcgttca
     4261 tctcttcggt tgactgcaca tccgacgaaa gcatcacaat catcaggaaa ttggaacgcg
     4321 ccttggatac ggttacgccg tattgctgga cggttgctgg cagcgtgctc aatacttcgg
     4381 aaagcttgtt ctgcacttcc acctgcgcca ggttctcgtc ggtatcgggc gtaaaggtca
     4441 ggctcacgct gccgctgccg ctcgaatcgg cagaagtgga catataatcc aaaccttcca
     4501 cgccgttcat attccgctcg atcacggaaa gcacgctgtc ttccattacc tgcgcggacg
     4561 cacccggata agtggcgtgc aaagtaatgg tcggggcggc gacggacgga tattgcgaaa
     4621 ccggcaggct tttgatgccg aaaatacccg ccgcaataat gaaaatcgaa ataacccacg
     4681 caaaaatagg gcggtcgata aaaaatttag ccatcgatgc cttccttatt tcgcttcaga
     4741 agcaggtttg gcttcagatg ccgtctgaac gccggattga ggcgaggcgg cttggttttc
     4801 agacggcgcc cattctttag gcgttacctt tttcgcaccc gttatgccga cgatactgat
     4861 gccttccaca accaccttgt ccccgtcctt cagacccgac gtaacaatcc aattcgtacc
     4921 ctgctgttgc gcaaccgtta cctcgcgggg ttccataccg ccttgggcat tcacaatcat
     4981 cacggtatct ttcgcaccgc gcgttaccgc ctgctgcggc acaacaaatg cgttatccac
     5041 cgccacttgg tccatcagca cgcgcacata caggccgggc atcaggatat tctgatcgtt
     5101 cggtacggcg gcgcgcaggg taatctgacc ggtcgattcg ttgacggtcg gatcggcaaa
     5161 cagcaggcgg cctttttcag ggtaaactgt gccgtcgtca aatttgatgc cgaccgcaat
     5221 cacaccatcc gccgccagca gtttgccttc ggctatctga cggcgcaatt tcatcacttc
     5281 ggatgcagac tgggtaacgt tcacatacat cggattggtt tggcggatgg ttgccagcac
     5341 ggtcgtatcg cccgcattca acagcgtacc ttcggaaact ttggactgac cgataaagcc
     5401 ggaaatcggc gcggtaatgc gcgaacggtt cagattgatg ccggcagatt tgatcgccgc
     5461 ctgtgctgct ttgacacctg cctcggcaga acgtttcgcc gttaccgcag catcgtattc
     5521 ctgccggctg acggcttcgg cggcaaccaa aggcttgtat cgcgccaaat ccgcatccgc
     5581 tttggcaagc gttgcctgag ccgttgccag ttgcgcgcgc gcgctttcca gatttgcttc
     5641 ataagtggaa ctgtcgatct gatacagcgg ctgtccggca cggacataac tgccttcttg
     5701 gaacaggcgt ttttggataa tgccgccaac ttgcgcacgg acatcggcgg tacgcagcga
     5761 ttccaaacgt cccggcaact cgacggtcaa cgcaacggtt tgcggatgga cggttacgac
     5821 accgacgacg ggagccggtg cttcccgacc cgcaggctgc ccgccctgcg ccgcgtctcc
     5881 gcctttaccg caagacgaca gtaccaatgc aacggcggca gccaacgcgg ccgcacgcat
     5941 cgccttaaaa gcataaaaag ccattattta tcctatctgt ctggtttgat gtaaagggtt
     6001 ttgccaatca acaggcatt
//
LOCUS       DAWKQJ010000119         6001 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000119 DAWKQJ010000000
VERSION     DAWKQJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.119"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(52..360)
                     /locus_tag="WJL20_001563"
     CDS             complement(52..360)
                     /locus_tag="WJL20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683701.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(344..646)
                     /locus_tag="WJL20_001564"
     CDS             complement(344..646)
                     /locus_tag="WJL20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ7683702.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(720..3083)
                     /locus_tag="WJL20_001565"
     CDS             complement(720..3083)
                     /locus_tag="WJL20_001565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ7683703.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3119..3637)
                     /locus_tag="WJL20_001566"
     CDS             complement(3119..3637)
                     /locus_tag="WJL20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683704.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVLLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3644..4708)
                     /locus_tag="WJL20_001567"
     CDS             complement(3644..4708)
                     /locus_tag="WJL20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7683705.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQATWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4885..5877)
                     /gene="pheS"
                     /locus_tag="WJL20_001568"
     CDS             complement(4885..5877)
                     /gene="pheS"
                     /locus_tag="WJL20_001568"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7683706.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1510 a         1554 c         1269 g         1668 t
ORIGIN      
        1 ttttttagcg tgtaaaacaa aaccggctgc atacctgcaa ccggcctcaa atcagcacaa
       61 ttccttatcc aaatccgcca acaggtcttt cagaccgtct ccgatttcct gaagcgtaac
      121 cggacggttg ccgtccgaac ccgattccgc accttctgca tacggtgcaa acgtgctctg
      181 caatttcaac agtttcacca catccaaacg ggtgtagcgt tcgccgccgt aaccgacaac
      241 cacaccgtga ttatgctgcc attgcgcaaa accatagggg ctgatttgca acagtccgca
      301 caactcgtcc agcgtgaaat agcgttttgc gggaaccttc aaatcaacgt tgtttgtcat
      361 agtagtgttc caccatgctt ttgagtttct ggctggcatg gaaagttacc acgcggcggg
      421 cggtaatcgg cacttcctcg ccggttttag gattacgacc cgggcgttgc ggcttgtcgc
      481 gcaactggaa atttccgaaa ccggaaattt tgatttcttc gccgcttgcc aaagtgctgc
      541 ggatttcttc aaaaaagagt tcgacgattt ctttggcatc gtttttggtg acgttgctga
      601 ctttgtctac caaaatatcg gccagttctg ctttagtgag agtcatatga ttaccttctg
      661 ttgtaacagt tgtggattat taccaatttt tatttaaaat tcaagcgaat atttattttt
      721 taactgcgaa gccgcgcccc tgccgccgtt gccgcgccaa tcagttttcc gataagcggc
      781 tcgaccgcct catccgtcag cgtgttttcc atatcctgca aaatcacttt gaccgccacg
      841 ctcttcatcc cttcgggcag tcccgtgccg cgatacacgt caaacacgct gatttcctgt
      901 accaacttgt ttgccgcgcc tttcaagaca agcagcaaat catcatggct catagcttcc
      961 ggcatcacaa acgccaaatc gcggcgcacc ggctggaatt tcgatacggc ccgatagcgc
     1021 gttttcccgc attccaacac ggccgccata tcgatttcaa ataccagcgg cgcttgcggc
     1081 aggtcgtatt tttgcagcca tttcggatgc agttcgccga caaagccgat gacgttgccg
     1141 tctgaaacga tattggcggc gcgtccggga tgcagggcag gatgtccggt tttaacgaac
     1201 tcgaccgctt tgtttttcaa cagattttcc acatctgcct taatgtcgta aaaatccgca
     1261 ttgcgcgttt tcccgcccca ttgttcgggc attgccgcgc cgtaccacaa gccgccgatg
     1321 cgttcgtttt ggacaaaccg gccgtctgaa cctttgccga acacgcgggc gatttcaaac
     1381 acgcgcacgc ggttttgttt gcggttcagg ttgttttgca aaatttccac caagccgccg
     1441 atgagtgtgg aacgcatcac ggcatattgc gccgccagcg ggttttgcag gcggatgggg
     1501 tcggcgttgg cggcaaaatc ttgttcccac tgctcgtcaa cgaaggcata gctgaccact
     1561 tcgcggtagc cgcgcgccgc catttcgttg taaacggcaa aacgcgggcg gcgtgtttcg
     1621 ggcagttcca gcattttcag acggcctgac gtgtaatcgt cggggatgtt ttcatagccg
     1681 taaacgcgtc cgatttcttc aatcaaatca gcctcaattt cgatgtcaaa acggaagctc
     1741 ggcgcggtaa cgcggaagcc ttccgccgtt ttctcgggct gcaggcccaa gtgttgcaaa
     1801 atggtttcca cctgttcggc aggaatgtcc acgcccaaca cggttttcag acggcccaaa
     1861 cgcaatccga cctgcttcgc ttcgggcaat tcgccttgcg cttccaccat ttcgcctgcc
     1921 gcaccgccgc aaatctgcaa caccaattcg gtagcacgct caatggcatc cgcctgcaaa
     1981 cggtaatcca cgccgcgctc gaagcggaac gacgaatccg aaccgaaacc gtattggcgc
     2041 gatttgccgg cgatgatttc gggcgcaaac caagccgctt ccagcacgat attttgcgtg
     2101 ccgtctgaaa ccgcgctcgc cgcgccgccc attaagcccg ccaaactcaa cacgcctttt
     2161 tcgtccgcca cgaccagcgt gttttcagac agggaaacgg ttttctcgtt caggcattcc
     2221 agcgtttccc cttcgcgcgc gcggcggatg tgcaggctgc cggaaagttt gtcggcatca
     2281 aaaacgtgca tcggctgacc gatttccagc atcacataat tgccgatgtc caccagcgcg
     2341 gaaatactgc ggatgccgct gcgctccaaa cgttgtttca tccaatccgg cgtagtagcg
     2401 cgcgcgttca cgttttcaat cacacggctg ataaagcgac cgcaatcggc aggcgcgtca
     2461 atctgcacgg gctgttttcg actgcccgtg atcggcgcgg tatggatttc gggctgcctg
     2521 aacgcgcacc ccgtcaatgc ggacacttcg cgcgcaatgc ctttgatgct caagcagtcg
     2581 gcgcggttag gcgtaatttt caacgtaaac agcgtatcgt ccaaatccaa gtattcgcgg
     2641 atattggtac cgacgggcgc atcttcaggc agaatgtgca ggccgttcac accgtcgtcg
     2701 ggcagaccga gttcgtcggt ggaacacaac atcccgtccg acaccacgcc gcgcattttg
     2761 gtcggcttga ttttgaaatt gcccggcaac actgcgcccg gcaacgaaca cggcactttg
     2821 atgcccgctt tcacattcgg cgcaccgcac acaatctgca ccaacccgcc cgtacccgca
     2881 tcaactcggg taacgttcaa acggtctgca tccggatgtt tttcaacgga tttcacttcg
     2941 gcaatcacca cgcccgcaaa cgcaggcgcg gcagtttcgg cctcttccac ttccaagccg
     3001 gacatcgtta acagatgttc cagcttatcg gaggaaagtt cggtatcggc ttgggttttc
     3061 agccatgaat aggagaattg catgttatgt tctcaattaa ctaattaact ttagattttc
     3121 aagagctagc taaacctttt tgaatggcta agtccctatt aattacttct acagaaatgt
     3181 tccataaaga aatcaaccgc aaagtatctt tcttattaaa ttcttgcttt attaattcat
     3241 tcaattgccc aagataatta agcagcacat ccgggataac cagtaataat ttgatttcta
     3301 tgctacccca ttgcaaattt gaaaaagcac taggcataat tgtttctttg atatcttctc
     3361 tgcctaatat tcccaaaatt tcaacattta atgcattaca ccatttttga taaatctctg
     3421 aaatatattc atcaaaacgc tcaggagatc tttcttgatt tggaatactt tgttttgcct
     3481 caacaaataa caaattatct tcttgcagat agataaactc tactgtctta ataccttctt
     3541 gccttctctt ccggatatat ggttctatat aaaacaggtt attttttact agattaaaag
     3601 ttatacctga ttcagtgaga tttattattt cactcataaa aacctaacca atccatgctg
     3661 tttccggaga taaaacacct atttcttctc tgaacaatcg aatagactca ccaataattt
     3721 cattatccgg catgctattt tgtaaatttt cctgcaccca agtagcctgt tccttttctt
     3781 tgccaggcat aaaaacagga atactttcac tatgctttct agctaataaa cacagcttct
     3841 tgataacaaa atatgaatgc gtactcataa aaatctgtat tcccaatttg gatttagata
     3901 aggaaaacag aatttctaga aattgattaa ttgctttcgg atgtagatta gcttctggct
     3961 catcaataaa taagattgat ccaggactca aaaaccgatt tcctaataga gtgtctaata
     4021 tcccaatctt ttttaccccc tctgctgtag aattgataga gaaagtttgt ctatcttttt
     4081 catataccca tgccttcttt ttatcattgt agctaacata accgccaaac aatgcttcta
     4141 gatcctttct agcttccgct gcctcagggt aattcctccc ttttgttggt ttaatatcca
     4201 atgcttttac caaatcaatg taagtagcat caaaaccaaa tctcttttct tcctctgatt
     4261 ttttaattac atcaaatagt gatagaactt ctttgggtgg taaaaaaata gagttagcgt
     4321 ttattctagg agaaagttcg tatagctccg gtttaattag ctttttggta gttctaccaa
     4381 aagaaaacaa aactgaagaa ctatcttcta aagtcatact taacttaaga ggccgttcgt
     4441 ttggattttc ttttcctctt gtaactaaat caccaatttc ctctacttgg aacgtccaat
     4501 atagcttatc tcgcaattcc tcagaaaaat cacgaatatt ctttccttta tgagcttcct
     4561 catgactttt taaaacacta tataatgcct ttaaaataaa agttttccct ttgctatttt
     4621 ctgcaataat taaatttatt ttccctaaat tatcagcctt caataagcta atcggtccga
     4681 atttttcaag ctcaaatgat ttaatcatat aatcccccta atatatatca acaatatttg
     4741 tacaagccac attgtaaaaa gacagcaacc acagcaccat agtaaacgca tagcaggcgg
     4801 ataatggagc gacatagcgt ttcatgattt agctttctga ggtaggcaca ggctgctttt
     4861 ttcattttta ggcagcctac acaatcattt tacaaactgc ttcaaaaaat tcaaatcgtt
     4921 atcaaaaaac aagcgcaaat cgttcacgtt gtaacgcagc atcgcgaaac ggtcgagacc
     4981 gataccgaaa gcgaaaccgg tgtatttttc agaatcgata ttcacatttt tcaacacgtt
     5041 gggatgcacc atgccgcagc cgccgacttc cagccatttg ccgttttcgc ccataatgtc
     5101 gatttcggca gacggttcgg tgaacgggaa gaaagacggg cggaaacgta cttgcaaatc
     5161 atcgcgttca aagaagcgac ggataaaatc cgtgaacact gctttcaaat cggcaaaagt
     5221 tacgccctct tctacccaca aaccttccgc ctgatggaac ataggcgagt gcgtggcatc
     5281 gctgtccaca cggtaaacgc ggccgggggc gataatgcgg atgggcggct cttttttatc
     5341 gagcatatag cggatttgaa tcggggaagt gtgcgtacgc aaaacatcgc cgttttcaac
     5401 gtaaaacgta tcctgcatcg cacgggcagg atggtttgca gggatgttca gggcttggaa
     5461 attgtgaaaa tcgtcttcga tttcaggccc gtccgccact tcgaaaccca ttccgtgaaa
     5521 gagttcgacc acacgttgca aggtcagggt tacgggatgc aggctgccgc cttcctgagc
     5581 gcgtccgggc agggtaatat cgagggcttc ggcggcaagt cgggcttgca gcttgacttc
     5641 gttgagggca tcgcgtttgg cattaaaagc cgtctgaaac cggtttttgc attcattgat
     5701 atgcgcacct atggttttgc gctcttcagg cgacatttgc cccaaagttt tcagaagtcc
     5761 ggtcaactcg ccggttttgc caagataacg ggctttgatt tgttctagag cgttgaagtc
     5821 ttgcgcagct tctactgcgg caatgccttc tgcaacgatg cggtttacat tttccataat
     5881 atcaagtctg tcaattaatg atatgccacc tgaaacaacg aggccgcctg tatagcctga
     5941 catatcagac tcttcagacg accttatttt cttcacggtc aatccattgg ataaattttc
     6001 t
//
LOCUS       DAWKQJ010000120         5921 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000120 DAWKQJ010000000
VERSION     DAWKQJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.120"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(272..379)
                     /locus_tag="WJL20_001569"
                     /pseudo
     CDS             complement(272..379)
                     /locus_tag="WJL20_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJL20_001570"
     CDS             complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJL20_001570"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ7683707.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1206..1949)
                     /gene="recO"
                     /locus_tag="WJL20_001571"
     CDS             complement(1206..1949)
                     /gene="recO"
                     /locus_tag="WJL20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ7683708.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVSFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(1983..3110)
                     /gene="pheA"
                     /locus_tag="WJL20_001572"
     CDS             complement(1983..3110)
                     /gene="pheA"
                     /locus_tag="WJL20_001572"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ7683709.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3149..4381)
                     /locus_tag="WJL20_001573"
     CDS             complement(3149..4381)
                     /locus_tag="WJL20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ7683710.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
     gene            complement(4901..5755)
                     /locus_tag="WJL20_001574"
     CDS             complement(4901..5755)
                     /locus_tag="WJL20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7683711.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         1383 a         1691 c         1600 g         1247 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tattacataa tcatcgcctc aatatttaat
       61 atcccaaaat atagatttaa aaccatcgcc tttgtggttt gtcagcattc ctaattgaga
      121 tagaaagtca aataatttct tgtttctccg gctgccatca agatggttta gattaagacc
      181 tcgaacaata caatacccaa tactctgtac tgtattgtga atttcttcta tcaaactatt
      241 attgtttgca gataaaatat acttttgatt catatagtga attaaattta aaccagtacg
      301 gcgttggctt gccttgccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
      361 ttaaatttaa tccactataa catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct
      421 gcccttacgg cagcagcctt gccctgaaca tcgcctcctt catctggcgc acggcttcgg
      481 gcagtccgag gaagagggct tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg
      541 cgaggatttg ggcgatgggg gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga
      601 ccaagcccaa atcgccggca aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt
      661 gttcggcgtg actgcgtgca tcggcatatg cgccggtgtg cagctcgaca acgggcgcgc
      721 cgacatcata ggcggcttgg atttgcctgt cgtcggcatc gataaacaaa gacacgcgta
      781 tgcccgcgtc ggtcaggatt ttggtgaatt cggcgatttt ttcctgttgc gccaatacgt
      841 ccaaaccgcc ttcggtcgtg atttcctgcc gtttttcagg cacgatgcac acgtcttccg
      901 gcatcacttt aagcgcgttt tcgagcattt cttccgtcaa cgccatttca aggttcaggc
      961 gcgtgcggat ggcgtttttg acggcaaaca cgtccgcgtc tttgatgtgg cggcggtctt
     1021 cgcgcaggtg catggtaatc aggtcggcac cgtgcgtttc ggcaaccagt gccgcctcca
     1081 cggggctggg ataagtcgtg ccgcgcgcgt tgcggacggt ggcgatgtgg tcgatgttga
     1141 cacccaaaag cataatgttt ccttttctct tcgtttttct gtttggcggc atggtgtggg
     1201 cgcagctacg cggtattgcg gtcaaactgc cttatctgtt ccaacacctg ccgcgatttc
     1261 aagccttcgg gaagcagcgt gccgatcagc agccgtgtga ttttcaatgc ctgttgcagg
     1321 ctttcggcgg tgcggaaact gccttcgcgc aaatcgatca ggctttgccc ttcgacaatt
     1381 gcgccggcct cgtgcgaaag cgcgtctgcg tcctcgcata cgggcatgac cgcctcttcg
     1441 ggcatcagcc ggtaggtttt gtccgccaaa atatcgccgc ccgtgccgtc ggcgtgcaaa
     1501 tcgggggcaa cgcccaaggc attgagcagc ttccactcga agcggcgcaa atcggcaatg
     1561 tggttggctt cacgacacac cgcctccatc acttttgcca acgcgtcgta cagttcgggc
     1621 atcggatctt cgcgcgcggt cagtttcaac accagttcgt tcacatacag accgctgaac
     1681 aacgccctgc cctgcggctg ccgccatccg ccgacccatt cggcacggtg aagggttttg
     1741 agttcctgac tgccgtacca cgacgcgctg acaggcacga aagaaaccag cacgccgcgc
     1801 aattcgctct gccgcttgcg cgcgcttctt gccaaaagtg ccacacgccc gtaacggcgg
     1861 ctgaaggctt cgacccgcag gctgctttcg cgccatggcg aggaagccaa cagaaagacg
     1921 ggttcgtggt tgactcggtg ttcggacatc atgcctgaaa tgccgtctga acgctgctgc
     1981 cgctacaaaa cggcggtcgg gtacgaaccg atgactttga cgaacgaagc gcgttcgccc
     2041 aagcgttcca atgccgtctg aatctgcgcg tcccggcggt gtccttcgat gtcgatgaag
     2101 aacaggtatt cccacaaaac ggatttactc ggacggctct caaacttggt catggaaata
     2161 cccgactcag tcagcggttg cagcagcgag gcaaccgcgc ctgcccggtt gggcgcggaa
     2221 acggccagcg aagtcttgtc gctgctgctt gcaccggttt cgtgatgtcc catcaccaag
     2281 aagcgcgtgg tgttgttcgg ttcgtcttcg atgcactcgg caaccatatc gagtccgtag
     2341 atttccgccg ccgtgcgtcc ggcgatggcg gcaaccgtac cgtcgtccga ttcggcaacc
     2401 agccttgcgg cttcggcatt gctggacacg gcaatccgtt cggcgttggg caggtgtctg
     2461 cccaaccagt cgttgcactg cgccaacgcc tgcgcgtggg aaaagacttt ggcaatgcct
     2521 tcggtgctgc cgttgttttt acgcaaaagg ttgtggtgga tgcgcaaaac gacttcgccg
     2581 cacgcctgca acgcggtaac ggcaagcaaa tccaacgtgc gcccgaccga gccttcggtc
     2641 gagttttcca cgggggcgac cagatagtct gcctgacggg tttcaacctg cttgaagcag
     2701 ttgtctatgg tcggacacgc catggtgtgc gcggcgtgtc cgaaatgctt aatcgccgcc
     2761 tgctgggtaa acgtgccctg cggccccaga taggcgatgg tcagcgggcg ttctacggcg
     2821 aggcactcgc tcatcacttc ccgaaacagg cgtgctaccg attcgtcggg cagcgggcct
     2881 ttgttcaaat cctgaatgcg gcgcaacacg gcgacttcgc gttcgggacg gtacactgcg
     2941 cccgtgcctt tcagttcgcc gatggcgtgt gcgtgttgcg cgcgttcgtt gagcaggcgc
     3001 aggatttccg catcaatggt gtcgatggcg ttgcggtggg gaaggaggag ttcgtcgata
     3061 gtttgggaca taatttcgcc tgaacagtaa tttatcgtgc attctaacat caaatgccgt
     3121 ctgaaacggt ttcagacggc attttccgtt aaaggtattc gctttgcccg ttttctttcc
     3181 acgcacgatg cgagcagagc cacagaagcg caatgccgca agaggtggac gcggtcatcg
     3241 ttgccgccat cacggttgcc gaaccgtcgt gcaagaaggt cgccgccata cccacccccg
     3301 cgccgattaa agattggaat acacccaata cggcgtttgc gctgccgccc tcttctttga
     3361 aataggacat aaaacacgcc tgcgtgtttg caccgaccaa gccctgcgta ccgacggaaa
     3421 acatcacgca cgcgaccagc agccaaaacg ggggcaaccc gaaaaacagc acggcggcga
     3481 gttgggacag gttggcggca aactggacga caatccccca tagcaggatg ctttgcggat
     3541 gcacgccggt tttgagccgc cacgcggtaa cgcggttgaa aaacatcatc gtgatgatgt
     3601 ttagtgcaaa cgcccaagcg tattgatgag gcgtaacacg gtagagctgc tggtacacga
     3661 aggaagattc ggtcagaaag gcgaacatcg aaccgaagct gaatgcctga aaaaacagat
     3721 aacccatcgc ggcacgggtt ttcaatacgc gcttgaaccg ccccgccacc agcccgaaca
     3781 cgtcccgtcc gattttgccg ccgacggcgg gcttgggcag gaaatactgt accaaaccga
     3841 gcagcaccag cgaatacgcc gccagaaaaa caaaaatcgc ctgccagcca cccaagccct
     3901 gcaacaatgc gccgaccatg ggtgcgacca gcggcacaac catcaaaatg atgccgataa
     3961 gggcaaacat ctgggcggct ttgcgtccgg aataataatc gcgcaccatt gcgccgacga
     4021 tgaccacagt catgcccgca ccgaatgcct gcacgacgcg caggttgagg agctgttcgg
     4081 cactcgaagc aaatacgatg gcggcaacgg caaggcagta gacggccagt ccggtcagcg
     4141 cgacgggttt gcgccctttg atgtcggaca ccgaaccgcc gaccacctgt ccgaacgccg
     4201 tgccgaacat aaacaaactc aaactctgtt cgatgcggtg aacatccgcg ttcagcgatt
     4261 gcgccatttc gggaatcgcg ggcaggtagg catcgatgga aaacggcatc agcgtaacca
     4321 gcatcgccat caaaaccgcc atcagttttt cgctcatttc aggataatgg gcagaaggca
     4381 taaattctcg tccgattcgc aaacaaaccg cccggcggcg gaaaacacaa tacgcggaaa
     4441 cggaaaattt taacacaccg gcacaaacag gcggcaacaa aagccccggc aaaaccggac
     4501 gaaccggaac gatatagtgg attaacaaaa accagtacag cgttgccttg ccttagctca
     4561 aagagaacga ttctctaagg tgcttaagca ccaagtgaat cggttccgta ctatctgtac
     4621 tgtctgcggc ttcgtcgtct tgtcctgatt tttgttaatc cgctataaaa tcccgtcatt
     4681 cctgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa ataaccgaaa
     4741 ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt cgttatttcg
     4801 gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag atggaaagat
     4861 ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga tgcgacagcc
     4921 ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac acgggcgaca
     4981 ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg agcgaaatca
     5041 acagtcccga cacgttggca ggaacacggc tcatcccctt gttccacagc caataggcgt
     5101 accagccgca ccccacgccc aaatacagca gcgacaatac cattccgacg ctccagtcca
     5161 cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat gcggcggcaa
     5221 tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc gtcggacgca
     5281 tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac cagccgactt
     5341 cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat gccgccgcgc
     5401 cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg acaaacacca
     5461 tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg tatttcaacc
     5521 cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc agcaacggct
     5581 tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt gcaggcagcg
     5641 cagcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca tagacatatt
     5701 tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa aacatagtga
     5761 gtgataaagt cagatggtca tacaaaagta tagcggatta acaaaaatca ggacaaggcg
     5821 acgaagcctc agacagtaca gatagtacgg aaccgattca cttggtgctt gagcacttta
     5881 gagaatcgtt ctctttgagc taagacgagg caacgccgta c
//
LOCUS       DAWKQJ010000121         5863 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000121 DAWKQJ010000000
VERSION     DAWKQJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.121"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..502
                     /locus_tag="WJL20_001575"
     CDS             <1..502
                     /locus_tag="WJL20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7683712.1"
                     /translation="NYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHA
                     RALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFV
                     PDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNGSYRLE
                     RLGTGK"
     gene            699..1589
                     /gene="rfbA"
                     /locus_tag="WJL20_001576"
     CDS             699..1589
                     /gene="rfbA"
                     /locus_tag="WJL20_001576"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7683713.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENF
                     RAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSL
                     SVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVET
                     RAKPLEKTAYGQYLLRLIGK"
     gene            1634..2191
                     /gene="rfbC"
                     /locus_tag="WJL20_001577"
     CDS             1634..2191
                     /gene="rfbC"
                     /locus_tag="WJL20_001577"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7683714.1"
                     /translation="MNIIDTALPDVKILKPQVFTDGRGFFMETFRDGWFKANIADRTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKAWAQAEKLRLTLSR"
     gene            complement(2235..4253)
                     /locus_tag="WJL20_001578"
     CDS             complement(2235..4253)
                     /locus_tag="WJL20_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ7683715.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIVIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(4449..5570)
                     /gene="dnaJ"
                     /locus_tag="WJL20_001579"
     CDS             complement(4449..5570)
                     /gene="dnaJ"
                     /locus_tag="WJL20_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ7683716.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         1551 a         1701 c         1371 g         1240 t
ORIGIN      
        1 caactacggt ccttaccatt ttccggaaaa actcattcct ttgatgattc tgaacgcgct
       61 tgacggcaaa ccgctgcctg tgtacggcga cggtatgcaa atccgcgact ggctgtttgt
      121 cgaagaccac gcgcgcgcac tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta
      181 caatatcggc ggccacaatg aaaaagccaa tattgaagtc gtcaaaacca tttgcgccct
      241 gctggaagaa ctcgctcccg aaaaaccggc cggtgtggca cggtatgaag atttgattac
      301 ctttgtccca gaccgccccg gccacgatgt gcgctacgcc gtcgacgcag ccaaaatcag
      361 gcgggatttg ggctggctgc ctttggaaac cttcgagtcc ggcctccgca aaaccgtgca
      421 atggtatctg gacaacaaaa cctggtggca aaatgtattg aacggcagct atcgtttgga
      481 acgtttaggt actggaaaat agttttcaga cggcatcccg acgcaatgcc gtctgaaaac
      541 ccatcgcaaa ggaagaaaga aaagatgttt caacacacag gacggcacat aaagcgtcgc
      601 cctatgtgcc gtcctgattt ggaaggggtt accccctccc aaataaagtc tgattctacc
      661 gccctaaagg gcggggtttc aaccgaaaag gaaatacgat gaaaggcatc atccttgccg
      721 gcggcagcgg cacgcgcctc taccccatca cgcgcggcgt atccaaacag ctcctgcccg
      781 tgtacgacaa accgatgatt tattacccct tgtcggtttt gatgctggca ggaatccgcg
      841 atattttggt gattaccgcg cctgaagaca acgcctcttt caaacgcctg cttggcgacg
      901 gcagcgattt cggcatttcc atcagttatg ccgtgcaacc cagtccggac ggcttggcac
      961 aggcatttat catcggcgaa gaatttatcg gcaacgacaa tgtttgcttg gttttgggcg
     1021 acaatatttt ttacggtcag tcgtttacgc aaacattgaa acaggcggca gcgcaaacat
     1081 tgaaacaggc ggcagcgcaa acgcacggcg caaccgtgtt tgcttatcag gtcaaaaacc
     1141 ccgaacgttt cggcgtggtt gaatttaacg aaaacttccg cgccgtttcc atcgaagaaa
     1201 aaccgcaacg gcccaaatcc gattgggcgg taaccggctt gtatttctac gacaaccgcg
     1261 ccgtcgagtt cgccaaacag ctcaaaccgt ccgcacgcgg cgaattggaa atttccgacc
     1321 tcaaccaaat gtatttggaa gacggttcat tgtccgtcca aatattagga cgcggtttcg
     1381 catggttgga caccggcacc cacgagagcc tgcacgaagc cgcttcattc gtccaaaccg
     1441 tgcaaaatat ccaaaacctg cacatcgcct gcctcgaaga aatcgcctgg cgcaacggtt
     1501 ggctgacgaa aaaagatgtg gaaacacggg caaagccttt ggaaaaaacc gcctacgggc
     1561 aatacctgct gcgcctaatc ggcaaataaa tgccgtctga accactttct tcagacggca
     1621 tacagagaaa atcatgaaca tcatcgacac cgccctcccc gacgtaaaaa ttttaaaacc
     1681 gcaagtcttc acagacgggc gcggcttttt tatggaaacc ttccgcgacg ggtggttcaa
     1741 agcaaatatt gccgaccgaa cctttgtgca ggaaaaccac tccaaatccg gcaaaggcgt
     1801 attgcgcggc ctgcactacc aaaccgaaaa cacacaaggc aaactcgtac gcgtagttgt
     1861 cggcgaagtg ttcgacgtgg ccgtcgatat gcgcgaaggt tcgccgactt tcggcaaatg
     1921 ggcgggcgca accctgtccg cgcaaaacca atgccagctt tggatacccg aaggcttcgc
     1981 gcacgggttt tgcgttttgg gcgacgcagc ggaagtcgtg tacaaatgca cggactatta
     2041 caagcctgaa gccgaacagg ttttaatatg gaacgacccg acagtcggca taggctggcc
     2101 gcttcaaacc gcgccgctgc tgtcgcccaa agatcttgcc ggtaaagcgt gggcgcaagc
     2161 cgaaaagctc cgccttacgc tttcccgata aaaaatgccg tctgaacgtt tcagacggca
     2221 ttttttccga caggctacct acccgccttc agtacgcgct gtgcaaagaa aaacatcccg
     2281 gtaacgaaga acgccaagcc caaccatgag gcggcggcat cccagttttg cagattcaac
     2341 gcaagcggcg catccgagcc gccgttggtc acggagaagg caataataaa cgccataatc
     2401 acaccgatca ggctttcacc gacaatcagg ccggcggaga acaaggttcc gatgcgctcg
     2461 gcgtttttca gacggccttc acggttttcc gcttttttac cgatgatgtg tttcaacacc
     2521 gccgccaaca ccgcgcctgc cacgatgggc atattgacgg acggcggcag ataaataccc
     2581 ataccgaccg caaggacggg cagggcaagt ttgccgcctg atgatttttt caacaccaaa
     2641 tcggcggcaa tcaacaccgc gccaatcacg ataccggtaa agatatagac ccattcaagg
     2701 ttgtgggcga aaatgcccga cgcgatggtc gtcatcaaag tcgcttgagg ggctgccaaa
     2761 gcctgcgccg cgtccatgcc ttcgcgcggc attgcgccgg taaagccgta ggcttcgtaa
     2821 agcagttcca acacgggcga aataaccagc gcaccaacga tacagccgat aatcagggcg
     2881 acttgctgcc gccaaggcgt ggctttgagc aggtagccgg ttttcaagtc ttgcaggttg
     2941 tcattggaaa tcgaagccac gcagattact gccgagccgc aaaacaaagt cagtgccagc
     3001 aaaaatttgc ggttagcctc atccgccaac aaacctccgg attcgcctac cagcagcaaa
     3061 accagtgaaa taacgacgac ggacacgatg cccacgccgg aaatcgggct ggaagacgag
     3121 ccgaccaaac ctgccatata accgcaggcg gcggcgacca aaaagccgat gacggaagcc
     3181 aaaagcgtgc aaacgaccac caaaagccaa gccatgccgc ccgtaatgtg cgaatcgccg
     3241 ataaagtggt aaaacgacac gcctaaaaca aacatcatag acaataccca gaaaatcatg
     3301 gctttaggcg acaaatcctg ttcagcgcgt tctgtagcgg gcgcaccgcc gccaaaactc
     3361 ttaaacgaca tcttcatgcc ttccaccatt ggcttgagca gcatcaaaag cgtccaaacc
     3421 gccgcgatac ctatggttcc cgcaccgata aaacgcactt tttccttcca cagcttcatc
     3481 gcaaacgccg ccatttccat atcggaaggt tgcggaatgt gtgaggagaa atacggcacg
     3541 gcaatgcccc aagcaatcga aatgcccaac aggatggcga tgccgcccgt cagtccgacc
     3601 aaatagcccg cgcccaacaa ggccagtgaa aagcccatcg gcagttggaa aattgccgta
     3661 ccgcttttaa accaataact cgcgctgtcg gcaatcacac gcagacctcc ggcacaaaag
     3721 ctcatcaatc ccgccaacgc gccgccggcc gccagctctt tgatgccgct gccgccctga
     3781 cggttatccc cttcttcatg accgcccact ttcaaaattt cagcagccgc cacaccttcc
     3841 ggataaggca aatcgctttt caccaccatc gcgtaacgca gcggaatggt gaaaatcacc
     3901 cccaaaatcc cgccggcaat acataaaagc gtcgtctgcc agaacgggaa accgctccag
     3961 tagcccgcca tcagcaaacc gggcaggacg aagatgatgg tcgaaagcgt acccgccgcc
     4021 gaagcttggg tctgcaccat gttgttttcc aaaatattgc tgcctttgaa aaactttaaa
     4081 accgccatcg aaatcaccgc cgccggaatc gacgaggcaa aggtcagccc gactttcaaa
     4141 ccgaggtaaa cgttggacgc agtaaaaatt acagtgatca atgcaccgag tatcatgcct
     4201 cggagcgtca gttcgcggta ttcttctacc gacccggata aagatttatt cattattctt
     4261 cctttgacaa cagacgttca catattgttg gcatcgcgcc atgatgtcaa gttttaaaaa
     4321 gaacagttaa aaacagttat cccaccctgc cccatacccc attgaaaata aaaactattt
     4381 ttaaacaaac aaaaacagcc gtatcaggga gattccccga tacggctgct tgtttccgaa
     4441 ccttaaaatc aatcaaacaa atcgcgcagc ttgtctaaaa acgatttctt gcgcggtgtt
     4501 tggttttcca agccggtaga aatccgctca aattcttcca aaagctcttt ttgacggtcg
     4561 gtcaaattga caggcgtttc gacaacaata tggcagtaca aatcgccggt cgcgctgctg
     4621 cgtaaagatt tgacaccctt acccttcacg cgcatcctcc tgccggtttg ggtttctttg
     4681 gggacggtga gcttgacctt tccgtccaag gtcggcactt ccaactcccc gcccaaagca
     4741 gccgtggcaa aactgatcgg cagttcgcaa tgcaagtcca gaccgtcgcg ttggaaaatc
     4801 ttatgcgccc gaatgcgaac ggttacatac aggtcgccgg caggcgcacc gtgcataccc
     4861 ggtccgcctt cgccgctcaa acggatacgc tgcccgtcat cgataccggc gggaatattg
     4921 acttccaccg tcttgaccgc cttattccgc cccgcgccac ggcatttgac gcaaggttct
     4981 ttaatgtgtt tgcccgcacc gtggcaggtc ggacaagtct gctgcatacg gaaaatcgcc
     5041 tgctggatgt gcaccgtacc cgaacctttg caagtcgggc aggtttccgg ggatgtcccc
     5101 ggtttcgcgc cactgccgtt acagacatca cacgcttcat aagtcggaat attgatgcgt
     5161 ttcttcacac cttttgcggc ttcttcaagc gtgatttcga taccgacttg aacgtcctca
     5221 ccctgataat caggctgggc gcgccccgaa ccgcctccaa acatttggct gaaaatatcc
     5281 ccaaagtcaa aaccctgcgc accgccaaat ccgccaaacc ctccgaagcc cccctgtccg
     5341 ccgccttcaa acgccgcatg accatactgg tcgtacatag cgcgcttttc cttgtcggac
     5401 aaagtttcat acgccttttg tacttcttta aacttctctt ccgcctcttt attgtcagga
     5461 ttgcggtcgg gatggtattt catcgccaat ttccggtagg cttttttaat ctcatcatcg
     5521 gtagctgttc ttgccacacc cagcgtcgca taaaaatctt gattactcat tttttcatct
     5581 aattcaaaat aaaatcacgg ctcaaaataa gggcaattgc gcaaaacaca agacaaacag
     5641 actgccatag cttacaaact gaaacggaat acacttttca gacagcataa accgatgccg
     5701 tctgaaattt tcaggtatgc acgacacaaa aacttagaca ggcataaaac caacccgcca
     5761 tgaagtgttt ttgttataaa aacgccgccc gaacgcatgt tcagacggca tttgatgcgg
     5821 ctgcagactt ccccttattt tattttttat ccgcgggcag cac
//
LOCUS       DAWKQJ010000122         5806 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000122 DAWKQJ010000000
VERSION     DAWKQJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.122"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            132..566
                     /locus_tag="WJL20_001580"
     CDS             132..566
                     /locus_tag="WJL20_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ7683717.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(579..1442)
                     /locus_tag="WJL20_001581"
     CDS             complement(579..1442)
                     /locus_tag="WJL20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7683718.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1450..2067)
                     /locus_tag="WJL20_001582"
     CDS             complement(1450..2067)
                     /locus_tag="WJL20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ7683719.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2230..2845)
                     /locus_tag="WJL20_001583"
                     /pseudo
     CDS             complement(2230..2845)
                     /locus_tag="WJL20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            3051..4823
                     /locus_tag="WJL20_001584"
     CDS             3051..4823
                     /locus_tag="WJL20_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ7683720.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4820..5476
                     /locus_tag="WJL20_001585"
     CDS             4820..5476
                     /locus_tag="WJL20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7683721.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1438 a         1631 c         1339 g         1398 t
ORIGIN      
        1 gttccgattc cgccgttatg tctgcctgcc ggacatacgt acgcattatt aacaaaagtt
       61 aaccgcgata ataccatctt tcacacgtca atctagtata tttcctaaaa tttccaacaa
      121 gaggaaaagc cgtgccactg cctgctccct gccgttttgc caaacctgcc gcctcttttt
      181 taagtatggc tttgctttcc tgtcagcttt cccacgccgc cacggcttat atccccccga
      241 acgattttca accgaactgc gacatacgcc gactcgggct gacccaaagt cagcacaatg
      301 agctgcgtaa aatccgcacc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
      361 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcctcg gatgttttta
      421 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
      481 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
      541 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
      601 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgaacg gctgccccgc
      661 gtctgcgccc actgcaatgc cgccatctgt gcggtttcat catacggcac gccgaaatct
      721 tccagccaat tttgcaccgc cgccagataa tcgttttgat cgaacggata aaaactgagc
      781 cacaatccga atcggtcgga cagggatatt ttttcttcca ccgcttcttt ctgatggatt
      841 tccccccgca tccccgtcgt accggcattc tcgtcaaaat attcgggcat caggtgccgt
      901 ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt gagacagacc gccgtctaac
      961 gcggttttca atgccttata ggtttcatcg ccgctttcaa acgacaaatc gtcgcaaaat
     1021 acgataaatt tttccggacg ttccttcaaa agcgtcaaca ggtaaggcag gccgattaaa
     1081 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg tagcagggct
     1141 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacatt gttcgcgggt
     1201 ctgccgacaa tgaactgttc ggtattacgc accagcaatt cggtttgcct gccgactccc
     1261 gccagcctta ccaagggaaa ggtgtgcgga tcgggcaagt gttccaaaaa accttttttg
     1321 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
     1381 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     1441 atctctgcct cactttgcat atctttgcgc catcagccgt tcgacggtat cgacgattgc
     1501 ctgcgtattc ggatcgattt cgatgttgat tctgtcgccg acctttctgc tgccgaacag
     1561 cgtccgttcc aaagtttcgg gaatcaaatg gacattgaaa cggctgtctt cgactttgcc
     1621 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     1681 ttcgtgcggg agcgaaaacc aaaccgtgcg gttgtacccg tcccgttcga tttcgacaat
     1741 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     1801 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccctaaat tggtttttgc
     1861 caaagtttcc gccattaaat cgaaactgac gcggtttcct tcgatttcgg taatcgtcag
     1921 gcagcagccg ttgttggcga ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     1981 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     2041 caagccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     2101 aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     2161 tccgattcgg gcaaaaaccg cccggtacgg cttgaccttt cctttccacg ccggtcggcg
     2221 gtcttaccct tatccctcct gcaaatcgat ttgcgtgttc aagtcggcaa aatgcccgtc
     2281 aaactcgaat ctgaccgacc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     2341 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     2401 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     2461 cacggtttca aacctcgcca ccaaatcgcc cggcagatac ggcatatcgc acggcacaac
     2521 caaaagccag tcggcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     2581 cgggccgaaa tgctgccact accgtgcgtc cggaaaaata tgcggacttc ttcgagcata
     2641 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     2701 gatgacatga tctatcagtg ccttaccccc ccaaaagagc aagccccttg tcctcgcctc
     2761 ccatacggct cgcctgaccg tcggccagta tcagggcaaa agttttcatt gcggatgttc
     2821 tcttggaaaa gttcgaggtt ttcatgattg cagtctgccg ttcccaatga ctcaaaatgc
     2881 cgtctgaagc agacggcaaa taaattcata ttatctgaat tttatcataa catgatttaa
     2941 cactgaagcg gtacggatta tgttttcctt aaccaattct ttctgagcag ttgtatctaa
     3001 tcccaaagaa tgatattgtt tgcattattt agaacaattt ttcgccgagc atgatactgc
     3061 cagcccgttt ttcagacggc atcagccttt ccctacgcct gaaactcctg accggagtgt
     3121 gggtcgggtt ggcggcattg tctgtcgttt tgacactgct gctctctttg cgtctggaaa
     3181 acgcggcctc cgtcatcgaa gaggcgggca acttgagaat gcaggcatac cgtctggcat
     3241 acatggcggg tgaaggatct ccccgctccc aaatagacaa tcaggttgac gaatttgaaa
     3301 aaagcctgaa tcgtatttca caaagtgatg ccatccatcc gctgattcct tcggacaccc
     3361 ctcttgctta tgatttgata caatccatgc tgattataga ttggcaggca cacatcctcc
     3421 ccccgctcca gtcctaccgg cgaccgactc aggtcgatct ctaccgcttt gccggaaaca
     3481 tcgaactgtt tttgcaggca ttggaaaatg ccaacgaaaa aaacacatgg tggctcaggc
     3541 gttttcaatg ggcaattatg ttgatgacgc tggtgtcgtc tgtactgatg ctgttttggc
     3601 accagatttg ggttatccgg ccgctgcagg cgttaaggga aggtgcggaa cgcatcggac
     3661 ggaggtgttt cgatattccg gttcccgaag gcggtacgcc ggaattcaaa caggtcgggc
     3721 gttgtttcaa tcaaatgggc ggcaggttga aaattttata tgatgatttg gaaggacaag
     3781 tcgccgagca gacacgcagt ctcgaaaaac aaaatcaaaa cctgaccctg ctgtaccaaa
     3841 ctacacggga cctgcaccaa tcctacatac cgcaacaggc tgcagaacat tttctaaacc
     3901 gtatcctgcc cgccgtagga gcagattccg gcagagtttg tttggacggc ggatccgatg
     3961 tttatgtttc cattcatcat gcggattgcg gcacagcagc ttcggatttg gggaagtacc
     4021 atgaggaaat cttccccatt gagtaccaga acgaaacatt gggcaggctg ttgctcagct
     4081 ttccaaacgg catttctctt gatgaagacg accgcatcct gcttcaaaca ctaggcaggc
     4141 aattgggcgt atcgcttgcc ggcgcaaaac aggaggaaga aaaacgcctg cttgcagtat
     4201 tgcaggaacg caacctgatt gcgcaaggat tacatgacag catcgcacaa gcattaacgt
     4261 tcctaaacct acaggtacag atgctggaaa ccgcctttgc cgaaaacaaa cgggaggaag
     4321 ccgcagaaaa catcagcttt atcaaaacag gcgtgcagga atgttatgaa gatgtccgcg
     4381 aactgctgct caacttccgt accaaaatca gcaataaaga atttcccgaa gccgttgccg
     4441 acctattcgc ccgctttacg caacaaaccg ggataacggt cgaaaccgcc tgggaaaacg
     4501 gttcgttcct gccgcctcag gaagcgcagc tccaaatgat ttttatcctg caggaaagcc
     4561 tgtccaacat ccgcaaacac gcccgcgcca cccatgtaaa attcaccctt tccgaacacg
     4621 gcggacgctt taccatgacc atccaagaca acggacaagg tttcgacacg gagaaaatag
     4681 gagaacccac gggcagccat gtcggactgc acatcatgca ggagcgtgcc aaacgcatcc
     4741 atgccgtttt agaaatccgt tcccaagctc aacagggaac caccgtctca ttgacggttg
     4801 catctgaaga aagcttgaaa tgactattaa aattattctg atagacgacc ataccctctt
     4861 ccgcagcggc attaaagccc ttttgtcgcg ccaacacggt tttgaagtca tcggcgaagc
     4921 cgcagacggc ctctcgggta tcaaaatgat cagtcggctg caacccgatg tcgtcctgct
     4981 tgaccttgat atgcccggta tgaacggacg cgaagcactc tcccaaatca tcagcatcaa
     5041 tccgcagcag gcagtgatta tgctgaccgt ttccgaagac agcgacgatt tgaccgaatg
     5101 tatgcgcatc ggcgcgcgcg gctacctgct gaaaaacatc aacgccgact ttctgctcga
     5161 aagcatacgc aaagccgctg aaggcgataa tgtattctcg cccgagatga ccgccaaact
     5221 cgtcaaaagc ctgatttccc cccaacctgc ccaagggacg caggcactct cctcacttac
     5281 ccctcgtgaa ctggaaatct tgggctatct cgccgcagga cacagcaaca aaatcatcgc
     5341 ccgccacctc gatcttgccg aatccaccgt caaagtccac gttcaaaacc tgctccgcaa
     5401 actcaacctc agcagccggg tacaggccgc cgtttacgcc atccggcaca acgtccccca
     5461 acctgtgccg gaataggcgt tcagacggca tattaggggt tttaatcccc gtacggtcat
     5521 tcggataaca gaccaagcat gtaagtttat gcccccataa gtacgcttgg catagcagta
     5581 atattgttcg gtttagtgtt ttccgtttgc ccctatctga tactgcaata tcagctatgc
     5641 cgtctgaaaa cgtatcatca tgatattttc agacggcata ataaaaagcg gaaatactaa
     5701 tgcggggtaa aatgttccat tccgaatccc ataaatatac aatggcttat atcgtttaag
     5761 catgtgtaac ccaccctcat atcaatcaat atatagtgga ttaaca
//
LOCUS       DAWKQJ010000123         5707 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000123 DAWKQJ010000000
VERSION     DAWKQJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.123"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(43..810)
                     /locus_tag="WJL20_001586"
     CDS             complement(43..810)
                     /locus_tag="WJL20_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7683722.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGILP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WJL20_001587"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WJL20_001587"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ7683723.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="WJL20_001588"
     CDS             complement(1777..2442)
                     /locus_tag="WJL20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7683724.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFLHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2621..4597
                     /locus_tag="WJL20_001589"
     CDS             2621..4597
                     /locus_tag="WJL20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7683725.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4665..5357
                     /locus_tag="WJL20_001590"
     CDS             4665..5357
                     /locus_tag="WJL20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ7683726.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETICETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1525 a         1805 c         1224 g         1153 t
ORIGIN      
        1 tccgcaaaag cgaatcatac tgccacattc cgacaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact tttatcgtta attttttcat
      181 agacacaatg cgccacagac aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 gatggcggat tggaacagcg cggctttgaa cggcagaatc cccaagcccg aagaagtccg
      361 tgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaaac
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcaaccgc cgtcaatacc
      721 gcgcaggttt ttcccggttt tgaacagcca gaccgccaaa cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gagcaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttcagtccta
      901 tttctgacag ttcgggcaat aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac cgggggcacg gctgattgtg ccgcccgtac actgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa atccctcaag gtactgccgc ccgtttcaat
     1081 ggcgcgctgc aacaccgctt tgacggtttc aaccaaaagc gcgcactctt tctttttcag
     1141 gcggttggca ggacggtggg gcgaaatgcc cgctctgaac aggctctcgt tggcataaat
     1201 gttgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc cttgcataca gataatccgc acaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc aacagcggat gatgttcttc gattccctca taccaaagta tcgcgccgaa
     1381 ctttctcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca aaatccccgt ttggaaacgg acaatcagat atttggcacg
     1561 cctgccgcag gacaaaacct gccgaccgga caaaatctcc cccaaatcgg gattgatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattcc ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaatcc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1861 cgcccttttg accaaaacca aaccgtacac tgcggcacac tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaacgg gcggcagata
     1981 caccataaat ttcccaaatt caatatcgaa accgacatcc gcaagctgcc ttttcaactc
     2041 gggcagcggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaaa cgtccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaagg aacaccgcct tccagagtat gcaggaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattttcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatccagata ccgcccgaag aaatcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgaatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2521 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg aatataaatc atgtccaaac tcaaaaccat
     2641 cgctctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatacgccc aaaacacctc
     2701 atcacaccaa atcggtttgg cgattatgcg cttaaactct tcaatactcg acctgccccc
     2761 gacaaaacaa tatttccaat ccggcagcct gtggagcgag ctgcgccaag gcttccggat
     2821 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccacag
     2881 ctatttcaac agggtcatca accggagtag accctatatg taccatatcg ccaacgaagt
     2941 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3001 caccaaagcc aaatcacacg tcggcgcatc gggcctgtgg cagttcatgc ccgctaccgg
     3061 caggcattac ggcctggaaa aaacaccggt ttacgacggc aggcacgaca tttacgccgc
     3121 caccgatgcc gcactcaact atctgcaata cctctatgga ctgttcggcg actggccgct
     3181 cgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccatca accgcgcccg
     3241 cgcccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3301 tgttcccaag ctgctcgccg tgcgcaacat cattgccgcc ccccaatctt tcggcatgaa
     3361 tatcagcgac atagacaaca aaccgtattt tcaggcagtc gaaccggacc gtccgctcga
     3421 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctaaaccc
     3481 cgcattcaac gtccccgcgt tcatccccaa aagcaaacgc aaactgctgc ttcctgtcgc
     3541 gtccgtacaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3601 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3661 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3721 cagcatcctt gtcgccaaga acggcaaaac ccttcagacg gcatcggaat ccgtcgtttc
     3781 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3841 cgtcggcatt gcccgaatcc gacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3901 tttgccgcag aaaaccgtcc gtacggaacc cgatcccctt gtccgtattg ccgaacctgc
     3961 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag accgccatgc cgtctgaaac
     4021 ccaaaccgca acactcgcgc agatcatccc ccaaaacgac atgcaggcgg cagacgaact
     4081 catgcagctt gttgcccgaa acaacctgcg ccgacaggct gaagaaacca tctccgccgt
     4141 catcggcacg cctgacacag ttgccgaaca caacatttcc tcatctccgc aacataccgt
     4201 tgccgccgac ggcaaacgcc gggcacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4261 cgaagccgaa atctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4321 gttcaacatt gccaaacgct acaacgtcag cgtagccgac ctgattgtcg ccaacaacat
     4381 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4441 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4501 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4561 caatccagga cagagggtta aactgattgg aagctgattc ggatacggca catgacagga
     4621 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa ctcatcaaat
     4681 acctgcaata tcaaggcata ggaagccgaa agcagtgcca atggctgatt aggggcggct
     4741 acgtctccat caacggaacc tgtatggacg acaccgatgc agacatcgat tcctcatccg
     4801 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4861 tcatgctcaa caagcctgaa gattacgaaa cttcgcacaa acccaagcac taccgcagcg
     4921 tattcagcct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     4981 atgcggatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacaacctga
     5041 cttcgccgag cagaaaaatt cccaagctat acgaagtaac gctcaaacac cccacaggag
     5101 aaacgatctg cgaaaccttg aaaaacggcg tgctgctcca cgacgaaaac gaaaccgttt
     5161 gtgccgccga tgccgttttg gaaaacccga ccaccctgct gctgaccatt accgaaggga
     5221 aataccacca agtcaaacgc atgatcgccg ccgccggcaa ccgcgtgcaa caccttcatc
     5281 gccggcgatt cgcacatctg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5341 aatgtccaaa attctgaaat aacatccaag aattccattt atattcatcc acatactcat
     5401 taaatatatg gtttaaccca atttaacgca aaaaatcaat taatgagtta tcaaagatta
     5461 ttgatgaatt tcaccatcaa catccagagc taaatgagag gattgcttta tttgatttaa
     5521 aaagaccaaa atttgaaaag gtgtgcctaa atcgtgttcg cttttttaca agaggttatc
     5581 aagataacgc caaccgtcca gaacccgtgg tttgcgaacc aatttgcaat ccaatatcac
     5641 cacagtttct aaggtgtgtt gaacattgat aacattattt gtaaaaatga tgaaaatttt
     5701 aacgctt
//
LOCUS       DAWKQJ010000124         5694 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000124 DAWKQJ010000000
VERSION     DAWKQJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.124"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(146..358)
                     /locus_tag="WJL20_001591"
     CDS             complement(146..358)
                     /locus_tag="WJL20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683727.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(370..651)
                     /locus_tag="WJL20_001592"
     CDS             complement(370..651)
                     /locus_tag="WJL20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683728.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(688..951)
                     /locus_tag="WJL20_001593"
     CDS             complement(688..951)
                     /locus_tag="WJL20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683729.1"
                     /translation="MADILPSDKRRQPPKTKGNPMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            complement(961..1595)
                     /locus_tag="WJL20_001594"
                     /pseudo
     CDS             complement(961..1595)
                     /locus_tag="WJL20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1570..1914)
                     /locus_tag="WJL20_001595"
                     /pseudo
     CDS             complement(1570..1914)
                     /locus_tag="WJL20_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1907..2782)
                     /locus_tag="WJL20_001596"
     CDS             complement(1907..2782)
                     /locus_tag="WJL20_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ7683730.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2827..3117)
                     /locus_tag="WJL20_001597"
     CDS             complement(2827..3117)
                     /locus_tag="WJL20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7683731.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3129..3308)
                     /locus_tag="WJL20_001598"
     CDS             complement(3129..3308)
                     /locus_tag="WJL20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7683732.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVIGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3422..3799
                     /locus_tag="WJL20_001599"
     CDS             3422..3799
                     /locus_tag="WJL20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683733.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3828..4073
                     /locus_tag="WJL20_001600"
     CDS             3828..4073
                     /locus_tag="WJL20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683734.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4307..5275
                     /locus_tag="WJL20_001601"
     CDS             4307..5275
                     /locus_tag="WJL20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683735.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5272..>5694
                     /locus_tag="WJL20_001602"
     CDS             5272..>5694
                     /locus_tag="WJL20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683736.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNL"
BASE COUNT         1332 a         1280 c         1430 g         1652 t
ORIGIN      
        1 gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt ctcagagtga gtttattttg
       61 gggcggcggc aggtcggggg caagcggcgt ggggcttggt tgtggttttt aggtttttgg
      121 gggtaaaaaa tgccgtctga acttttcaga cggcgtttgt tttttctatc caatcgagga
      181 actgccgcca tttttccagc ggcatatcgg cccgccgcgt ttcggtatcg ggttcggctt
      241 cccagcggcc tacctgtatg tagtgcttca cgccgacgat ttgcgccaac tcggcctgcg
      301 tcagtttgca gcggttgcgc agggtgcgaa ggttgtaagg cgtgtagccg agttccatgt
      361 cgttgcggtt tattttgttt cgcatatttt tttgactgcc cggcggcagg tttcggtaag
      421 gacggcggca aatcgggctt cgtctgccgg tttgccgtcg aaaaaaataa aatcgtaaag
      481 ggtgatgccg ttttcggaat ggcggtaatg cccgcccggg cattcgccgt ctgtctgcct
      541 gtggattttg gcaatcaggc ggggatagcg gcaatgggta atgaaacggc ttgcgccgtc
      601 ttgcccgata atccattcgg ggtagcggtt gaatagtgcg gctctgttca ttttgttgag
      661 ggataaagcc cctcgcgggg cttgtggtca ggcaaatttg aatatcagtg ccaacacggc
      721 ggcgatggcg gtaaccagcc cggtggccgc tatcatggga taccagcggg actcttgggc
      781 tatttttacg gattcggcgt tgattttgtg cgcgtctgca atgattttgg cgatttcggc
      841 atctactttt ctcagactgg agtgtttcat ttcttgttcg attatgttca ttggatttcc
      901 tttcgttttt ggcggttgcc gccgcttgtc ggatggcagg atgtctgcca tgtgtgtaca
      961 ttaatacctt ttaggtttta ttgcaagtgt tttgggcagg atgccgtctg aatttttttc
     1021 aggcggtttt ttgttttgcg gggttttggc ggcggcttcg tatgcttggc ggtggcggcg
     1081 gctgtaccat tcggcgagtt cgcgccgctc ttggaggcgg tagctgtcgg cggtcaggta
     1141 gtggtgcagg cttgagaagt cggcgcgttg gagggcggcg cggctgatgt ggccgagggc
     1201 gaggtctgtt gtgagggtgt cttttccggc ggtcaggctg atgttggtga agaggtggcg
     1261 gaatccgtgc attgtgtgtt tggatttgcc gggggtgctg ccgtcatagc ccagtcgtcg
     1321 gatggcgttg tgggcgaatt tgatgctgat gtggtcggga tgggggcggg tttgcgccgt
     1381 gggcggatgc ctgggaaaag gtggatgttg tcgccggtct gtgtgtgcag ctctcggagt
     1441 atttctactg cccagtccga cagtgggacg gtaaaggggt gtttggtctt catgtcggcg
     1501 gcggggatgt gccataaccg ggcggtgagg tcgatgtctt gccagcgggc ggaaagcagt
     1561 gcggagggac gggatgactt ccccggtttc cccatcaatc cgcgccgcct cgatttttcc
     1621 gttggcgcgc agcatttcca ccgcctgccg caccgacatc aaccgcttgc cgttgcgcct
     1681 catcgcctcc accaaaaccg ccgaaatcaa tttggcttct tcctgtttaa gctccgtctt
     1741 gcccgcatcg ctgcgccgtt tgcgcgtcgg cttgacgctg accgcctcca gcttgcggta
     1801 tagcgtggca aggctgatgc ccaattcctg cgcctgctgc ttaagatatg cagagcgtgc
     1861 gccgcgttcc attgcttccg cctgattctc gactgcctta agacgctcaa tcattgccgg
     1921 attcatcgcc ttctcccgtt tcaccgccca accattccgg cacattgtct gtcggtgctt
     1981 cagtcggcag ggcatagctt tcgcgcagtt gctcgcagtc caaaataatt tgattgagcg
     2041 tgccgaccat ctttgcctga tggctgatcc cgtgtgcctc actgtgcgca ttaagttggt
     2101 cgaacaaatc tttcagacgg ctcacttgac tgcggatacc gacctcaagg cttgttaact
     2161 gcatcgccaa ctcgctgcct acgtcttccg ccttcggctc tctgacaacg gtttgcttct
     2221 tagccagctt ctcggccagc tcatcaattt tggctgtttt ggttttcatc acttcgtctt
     2281 tggcggcgag gttttcgcgg ctttcgcgca gggcgacgcg cagctcgcgc accgtcattc
     2341 ggtccacatc gtcaaaggtc atgccgttga cttcttcccc ttcggccaaa cccaccagcg
     2401 taacgtcttc ttcgaccagc agctcaagca gcttcgactt gcccaaatcc atcagcttcg
     2461 gcgcggcttt ctgcatttgc ggggtcgcaa agcggcgagt ggctgacatc agacgtgatg
     2521 tttctgcgat acccagccca aattggcttt tcacaatttc cataaaccgt ccgtgttccg
     2581 tatgctcttt taaaataatc agcgcacgtc ccagttcgaa catgccttcc atcgtctgcc
     2641 gtactgcttg acgaccgcgt tcaacccaac gctcttcgct ataagtctcg ccgttacccc
     2701 actgctccat caccagcaca ctgcgcatcg ctgcctgatt gcttactttg tcggttgcga
     2761 taatttcaat ttctgtattc attttttatc tccaaagttt ccgacgtcgg aaactttcaa
     2821 aatccgttaa tccacatcga cccgcttgcc gatttcggca atcttgcttt gcagccgttc
     2881 atgctgctgc ctgaaccgct ctgcgatttg cagggttttg atgccgtagg cgtagttgcc
     2941 gttttcaagt ttgatgacca atcccgaggc aaccaaatca tcaatatccc tgctgacttg
     3001 cgatggcgtc agccccagtc cgaccgataa atccttattg ctcagaccga taatcggatg
     3061 ctcgtcaagc gcgataaaga ccctcaatag ccgttgtacc cttttacttt ctgccatccg
     3121 catcctcctt atttcagtcc cagcttcttg gcaatttcat gccccttgcc gtaattgcct
     3181 ttgcgctgtc cgccgatcac tagatacaca tcgcgcggct taaagccgtt ctctcttgcc
     3241 cactgcgcca gcgtctggcc gttttgggca aaattttctt ttagtttttc aacagtatag
     3301 ccatagatag ccctttccca ttgtgataaa atagtgaact atttatgcac tagaaattaa
     3361 ttagtgcatt accaatgcgt gaattatgag attttaaaat ctcttttgta aggattattt
     3421 tgtgatttct aaatctcttt ttggtaatcg tttgaaagaa aaaagaaagt ttttagggct
     3481 tacccaagca caagctgctg aaaaagcagg tattgagcgc gaaacatggg gcaaatacga
     3541 acgtggcgta tttatgccta gtggagatgt attgctctct tttttgaata tgggaataga
     3601 tgtgagcagc ttatttgctg ctgaacaggg aattaggccg tctgaaaatt ctgaaattag
     3661 taaggaagag tcagagttat tagggtatta ccgccaagcc agcgataacg gcaaatttgt
     3721 cattctcagt gtggcaaaag gcgcagagaa aaaagcagcc gaaaatactc aagtttcagc
     3781 cacgcataaa acagcgtgat ttttaaatcc aaaatggaga cagtcttatg tttgactttg
     3841 aacaacaaat caaatggggt gagcgagcgg aagaaatcgt taaagaggca gccacccaga
     3901 acaatatcga aattcccgaa cctttggctt cggcattagc taaggcggta aaagtgcatt
     3961 atctaagcca agctggtgtg ttttctttgg tagaagccta tgcagacacg gtaaatccaa
     4021 ctgaaaaaga agtagattac caagcaatcg gcaaagagct atttgaaaaa taaaaaatgc
     4081 cgtctgaaat tttcagacgg catttaggac actccgacag tcgggctgct ccggtgttct
     4141 gaaacggata ttaagcaatt gaagcggccg tgcattttaa aacgcattaa aaactacccc
     4201 gacatacagg tcatcgggca ggtggttcag gtgttgaaag atttgaattg atgccgtctg
     4261 aatttatttt ttcgacacag atattaaaag agaaaggact gtacaaatgg caaaaaaaac
     4321 tactaaaact attcattatt tacgtgcgca gcaaaaccat agcttgttcg atttagaggt
     4381 agcattaaga agggttttgt cagctgctcc tactgttcaa gatacacaaa ttgaacaata
     4441 caatcagctt acccaaatca tgcatagaac tttggatcct aaagatgtaa aaggcaattc
     4501 aattggtgga ttactgatac acattggcag cggtacaaaa gatgaacata tccgcactat
     4561 gagcaataga cctgtacagc aagatgatca tggtggaaca caagctcctc caagtggata
     4621 ttcctttttg cgtaaagagg cttttttgta tattgttgga caccatgtaa ttttttgcgg
     4681 acatggtttt ttgtcagcat caacggttgc ttcttattta agtttactta gcaataaatt
     4741 gagagagaac aatccggata ttgttcaatt taatattgaa ttcaaagcag taggcaattg
     4801 cgacaagttg tcattgatac aacaacatgg ggtaaaaagt attcttttag atgcttcggc
     4861 atatcaatta tccagaaaca gactgtatca agatagccgt tcaaccattg ctaaagcatt
     4921 agggaaagtt ggtagtgtat ttacaagcga attgagtgat gaggaattgg aagcacaatc
     4981 cgaaattcat atcaatttag aagtcttgct aaacggtaat tcgagagcca gtattgaagc
     5041 gcaatcgctg atgcaggagc aggcagagga aattattgat gatgaaacgg ttaatcaagg
     5101 attttccatt accacgcaac aaggtgaagt gattcagccc tctgatgtaa aactgtctaa
     5161 atctgttaga ataggacgat acgatgaagc caactcgtta ctccctgact cggcatttac
     5221 agcaatcagt gaatatttcc tagaactcca aagcagaaac ttaactgaac aatgaaaaaa
     5281 tatctgccct tactacgttt tttgttgatt gcttgtatat cagcattttt agcatggaaa
     5341 gggcaacctt ttgttcatgg gaacgagaag gccgttgatt taattattaa cgtatttgca
     5401 atcttagccg gttttttaat tgccattatg acattgttta gtgatatgag gtttgatgaa
     5461 gatgcaaatt ggcgacaaat tcaaattcgt gaaggcgtac aagaacaacg gtatataaaa
     5521 cattccttac ttttttacac atatcttgcc gtattagttt gtgtttttat tgtcatcttg
     5581 ttggctcata aagaagaata caaaaatgga ccagccattt tttggcttga acggagctat
     5641 ttatttttag cctgtatttc cattttttat tctgtattct tacctgggaa ttta
//
LOCUS       DAWKQJ010000125         5651 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000125 DAWKQJ010000000
VERSION     DAWKQJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.125"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..889)
                     /locus_tag="WJL20_001603"
     CDS             complement(128..889)
                     /locus_tag="WJL20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683737.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            989..1240
                     /locus_tag="WJL20_001604"
     CDS             989..1240
                     /locus_tag="WJL20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683738.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1292..2098
                     /locus_tag="WJL20_001605"
     CDS             1292..2098
                     /locus_tag="WJL20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ7683739.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2405..3928
                     /locus_tag="WJL20_001606"
     CDS             2405..3928
                     /locus_tag="WJL20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ7683740.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4026..5438
                     /gene="trkA"
                     /locus_tag="WJL20_001607"
     CDS             4026..5438
                     /gene="trkA"
                     /locus_tag="WJL20_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ7683741.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1412 a         1704 c         1370 g         1165 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat attaatattt tgcgcctgtt
       61 ctatgatgct ttcaagtcgg gtgagaatgc aaatgccgtc tgaaacggct ttcagacggc
      121 atggcaatca gcgtttgtat tttaactcgt acttgatgtc gttgaggatt ttgcggacat
      181 cgtgttccaa cacgtcttcg actaccgcac ccgcctgctc gtgcagcatc tgctggagct
      241 gataggtaaa aaccgccatc tgcttttgca ccgccgtacg gatgatgccg ttgacggtat
      301 cggtcagatg cgggcgcagg cgtttgatca gccgttcggt cagctcctgt tcggacaggc
      361 agaacacttc gcgccggttg acggctttcg gattcaggat attgatttgg acgggcatca
      421 acgcctcttc cgcaccgcct tcgccgtttt ccgcaacccc gtctgccgcc gtacccgatt
      481 ctgccgcatc ggcgttttcc ccgccttcaa tctgtccggt ttcaaattcg gcactgtctt
      541 ttttggcatc gaacaggatt ctccgccgcg attcgatgtg tttttccgaa accgacattt
      601 gcagggaagc ctgcgcgttg agccagtttt cctgaaggac aatcatcggg tcggtttcga
      661 cttcctcgcc gcaatcggca acggcggcat tgtgttcctc ctgccatttt ttcagatacg
      721 ccttcaacac acgggctcgg ctctcatcgt ccagtttcgg cacaggcgcg tccgttccgg
      781 tttcagaggg gcgggacagc ggcgcgtaag tcggcactgc cttcatacgg cgcgtctgac
      841 gcaggttttc caaacgtttt tcccaattcg gctctttatt cgcatccatt ttcggcttcc
      901 ggtccttaat ctttgcaagc agacaaaccc gcgcccaaag cgcggtttga tataatggcg
      961 cattttaaca gattcgcgag gatacatcat gggcagcatc gaacagcgtt tggaatatct
     1021 ggaagaggcg aacgacgtgc tgcgtatgca gaaccacgtc ctgtccaccg ccttcaaagc
     1081 cttaatccgc gcccttcccg cagacaccgc cgaagtcgcg gtcgagtcga ttcagcttgc
     1141 atttgaagac gcactggcag aattgagcta tgaggacagc ccgcatacgg atttgttcca
     1201 cgacgttact tatgcgtttt tccgtgaaaa ggaacgttaa ttttatgtta aactgatttt
     1261 ttaggctttt tgattaccga aaggaatttt gatgaatatg aaaaaatgga ttgccgccgc
     1321 ccttgcctgt tccgcgctcg cgctgtctgc ctgcggcggt cagggcaaag atgccgccgc
     1381 acctgccgca aaccccgaca aagtgtaccg cgtggcttcc aacgccgagt ttgccccctt
     1441 tgaatcttta gactcgaaag gcaatgtcga aggtttcgat gtggatttga tgaacgcgat
     1501 ggcgaaggcg ggcaatttta aaatcgaatt caaacaccag ccgtgggaca gccttttccc
     1561 cgccttgaac aacggcgatg cggacgttgt gatgtcgggc gtaaccatta ccgacgaccg
     1621 caaacagtct atggacttca gcgacccgta ttttgaaatc acccaagtcg tcctcgttcc
     1681 gaaaggcaaa aaaatatctt cttccgacga tttgaaaaac atgaacaaag tcggcgtggt
     1741 aaccggctac acgggcgatt tctccgtatc caaactcttg ggcaacgaca acccaaaaat
     1801 cgcgcgcttt gaaaacgttc ccctgattat caaagaactg gaaaacggcg gcttagattc
     1861 cgtagtcagc gacagcgcgg tcatcgccaa ttatgtgaaa aacaatccga ccaaagggat
     1921 ggacttcgtt accctgcccg acttcaccac cgaacactac ggcatcgcgg tacgcaaagg
     1981 cgacgaagca accgtcaaaa tgctgaacga tgcgttggaa aaagtacgcg aaagcggcga
     2041 atacgacaag atttacgcca aatattttgc aaaagaagac ggacaggccg caaaataagc
     2101 ccgcccgtcc gaacacaatg ccgtctgaag ccctttcaga cggcattgtt catcaatcgg
     2161 cctacaatga actgcctgct gatttctccc taccgcaaag caacaggcaa agattacaaa
     2221 tatcaaaatc cgagtaaaac agtattttat taaaacaaat tgataatcaa gagattagaa
     2281 ttatgtattg tctttaccgt acaaacgctg gcactatttc aacctgataa aaaacagcct
     2341 tcaaaaaggt tgtttaaaac agcagcagac acttaccgcc acaaccttga aaaggaacac
     2401 aatcatgacc gtcatcaaac aggaagactt tatccaaagc atttgcgatg ccttccaatt
     2461 catcagctac taccatccca aagactacat cgacgcgctc tataaagcgt ggcagaagga
     2521 agaaaacccc gccgccaaag acgcgatgac gcagattttg gtcaacagcc gtatgtgtgc
     2581 ggaaaacaac cgccccatct gccaagatac cggtatcgca accgtgtttt tgaaagtcgg
     2641 tatggatgtg cagtgggatg cggatatgag cgtggaagaa atggttaacg aaggcgtgcg
     2701 ccgcgcctac acttgggaag gcaatacgct gcgcgcttcc gttctcgccg accccgccgg
     2761 caaacgccaa aataccaaag acaacacgcc cgccgtcatc catatgagca tcgtgccggg
     2821 cgacaaagtc gaagtaacct gcgcggcaaa aggcggcggc tctgaaaaca aatccaaact
     2881 cgccatgctc aatccttccg acaacatcgt cgattgggta ttgaaaacca tcccgaccat
     2941 gggcgcgggc tggtgtcctc ccggcatctt gggcatcggc atcggcggca cgcccgaaaa
     3001 agccgtgttg atggcaaaag aatccttgat gagccacatc gacattcaag agttgcagga
     3061 aaaagcagcg tcaggtgcag aattgtccac caccgaagcc ctgcgcctcg aactctttga
     3121 aaaagtcaac gcgctgggca tcggcgcgca aggcttgggc ggtctgacca ccgtgttgga
     3181 cgtgaaaatc ctcgattacc cgacccacgc cgcctccaaa ccgattgcca tgattccgaa
     3241 ctgcgccgcc acccgccacg tcgaatttga attggacggc tcaggccctg tcgaactcac
     3301 gccgccgcgc gtcgaagact ggcccgattt gacttacagc cccgacaacg gcaaacgcgt
     3361 cgatgtcgac aagctgacca aagaagaagt ggcaagctgg aaaaccggcg acgtattgct
     3421 gttgaacggc aaaatcctca ccggccgcga tgccgcacac aaacgcctcg tcgatatgct
     3481 caacaaaggc gaagaattgc ccgtcgattt caccaaccgc ctgatttact acgtcggccc
     3541 cgtcgatccg gtcggcgatg aagtcgtcgg tccggcaggt ccgaccacag ccacccgcat
     3601 ggacaaattc acccgccaaa tgctcgaaca aaccgacctc ttgggcatga tcggcaaatc
     3661 cgagcgcggc gtggccacct gcgaagccat cgccgacaac aaagccgtgt acctcatggc
     3721 agtcggcggc gcggcgtatc tcgtggcaaa agccatcaaa tcttccaaag tcttggcgtt
     3781 ccccgaattg ggcatggaag ccatttacga atttgaagtc aaagacatgc ccgtaaccgt
     3841 cgccgtagac agcaaaggcg aatccatcca cgccaccgcc ccgccccaat ggcaggcgaa
     3901 aatcggcatc atccccgtca aatcttgagg cgtcatgccg tctgaacaca aaatctgctt
     3961 tcagacggca tttccgcccc tggttgcggt acaatccacc atttcatcac tcggcgaccc
     4021 acaccgtgaa aatcctcatt ttaggcaacg gacaggtagg ttctaccgtc gcacaaaacc
     4081 ttgccgccat acccaacaac gacgtaaccg ttatcgacat cgacgaaaaa gcattgcagg
     4141 aaacaggcag ccgcctcgat gtccaaaccg ttttcggcaa cggcgcatcc cccttcacat
     4201 tagaacgcgc cggcgcggaa gatgccgact tgctgctcgc gctctcccgc agcgacgaaa
     4261 ccaacatcgt cgcctgcaaa gttgccgccg acctgttcaa catccccggc cgcatcgcgc
     4321 gcgtccgttc cagcgaatac ctcgaatacc tcagccccaa gctcgaaaac aacgaaaacg
     4381 gcagcctttc catattcggc ataaccgaaa ccatcagccc cgaacagctc gttaccgaac
     4441 agcttgccgg cctgatagac tgcccgggcg cattgcaggt tttacgtttt gcagacgacc
     4501 gcgtgcggat ggtcatcata caggcgcggc gcggcggact gcttgtcgga cgcagcattg
     4561 ccgacatcgc ccaagatttg cccgacgggg ccgactgcca aatctgcgcc gtttaccgca
     4621 acaaccgcct catcgtcccc gcgccgcaaa ccgtcatcat cgaaggcgac gaaatcctat
     4681 ttgccgccgc cgccgaaaac atcggcgcgg tcatacccga attgcgcccc aaagaaacca
     4741 gcacccgccg catcatgatt gccggcggcg gcaacatcgg ctaccgtctc gccaagcagc
     4801 tcgaacacgc atacaacgtc aaaatcatcg aatgccggcc gcgccgtgcc gaatggatag
     4861 ccgaaaacct cgacaacacc ctcgtcctgc aaggttcggc aaccgacgaa accctgctcg
     4921 acaacgaata catcgacgaa atcgacgtat tctgcgccct gaccaacgac gacgaaagca
     4981 acattatgtc cgcccttttg gcgaaaaacc tcggcgcgaa gcgcgtcatc ggcatcgtca
     5041 accgctcaag ctacgtcgat ttgctcgaag gcaacaaaat cgacatcgtc gtctcccccc
     5101 acctcatcac catcggctcg atactcgccc acatccggcg cggcgacatc gttgccgtcc
     5161 accccatccg gcgcggcacg gcggaagcca tcgaagtcgt cgcacacggc gacaaaaaaa
     5221 cttccgccat catcggcagg cgcatcagcg gcatcaaatg gcccgaaggc tgccacattg
     5281 ccgccgtcgt ccgcgccgga accggcgaaa ccattatggg acaccatacc gaaaccgtca
     5341 tccaagacgg cgaccacatc atctttttcg tctcgcgccg gcgcatcctg aacgaactgg
     5401 aaaaactcat ccaagtcaaa atgggctttt tcggataaac cgccccattc cggacacatt
     5461 gccgccaagc ggtatggaag cggaataatg gtaggtgggc ttcagacggc atcgtccccc
     5521 accgttattc ccgcgcaggc gggaatccgg tctgttcggt ttcagttgtt tttgggtttc
     5581 gggtaatttc caaatcgtca ttcccgcaca ggcgggaatc caaaccattg gacagcggca
     5641 atattcaaag g
//
LOCUS       DAWKQJ010000126         5620 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000126 DAWKQJ010000000
VERSION     DAWKQJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.126"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..148)
                     /locus_tag="WJL20_001608"
     CDS             complement(<1..148)
                     /locus_tag="WJL20_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ7683742.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDH"
     gene            complement(372..2504)
                     /locus_tag="WJL20_001609"
     CDS             complement(372..2504)
                     /locus_tag="WJL20_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ7683743.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3286..3939)
                     /locus_tag="WJL20_001610"
     CDS             complement(3286..3939)
                     /locus_tag="WJL20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7683744.1"
                     /translation="MKTTHCKLKKKVQKELLRFFVLEVTARSAADILGIQPDSAILFY
                     RTIRMVISHYPALAADEVFEGSVELDESYFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNARSETLLPVIKRKIMPDSIVYTDSLSSYDKLHVSGFIHHRINHSKEFADRQ
                     NHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            4276..4710
                     /locus_tag="WJL20_001611"
     CDS             4276..4710
                     /locus_tag="WJL20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683745.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQPYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4751..>5620
                     /locus_tag="WJL20_001612"
     CDS             4751..>5620
                     /locus_tag="WJL20_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7683746.1"
                     /translation="MIGSGDTKQCKKFSADEFAKTIQSNFQSGIKYDGPTLATAGVSN
                     IIDEYLKDAYGGNGLKEAQRNLVAKEIADRIEAELHRQRANYQAQEAAKYSPAHGGVE
                     KSMYKHHADLQKTNTNIANRYSASANEYAAQAKSLGSLPKSVGYAALKNLGNALAALQ
                     MGEALYSNKANNIAKTGSSILGGMLAVAAAGAIASLAGVTLPFWASIAVGASIGLGIS
                     AAWDAFGVGEKLGLDPNVPAFDPFFGWLYDKTGGWEGGIPDWAKEWLGLNRDGKYHQY
                     DPLTLDLDGDGIET"
BASE COUNT         1663 a         1266 c         1251 g         1440 t
ORIGIN      
        1 ggtggtctcg aaggtaacgg gttttttgac ggttcaggta ttgttcgatc ggctgccaat
       61 caatcacttg atccaacttc aatagcggga agcggttgat gtgtttggca atcatggctt
      121 gtgcggtttg ccggaagaag gtgctcatgg aaaatcccct aaatggcttg gtgggaattt
      181 aggggatttt ggggaatttt gcaaaggtct ctagatgagt gaaaaagaag tgcaggctgc
      241 ctaaaaagac agaaaaagtc tttccggcag cctgcacttt ggtttcattt cagtcagtaa
      301 acccagtaaa cgacggtcta aaaacgcaga acgttacgaa aaaagcagcc tacacgccca
      361 tcccccgcct tctacccgtt ctgtaaatca tacagatagc ggtaatatcc gttcggcttc
      421 gccagcaatt cctgctgtgt tcccgcttcc acaatcctgc ctttatccat ggcaatgatc
      481 cggtgtgccg ttttaacggt ggacagacgg tgggcgataa tcagcaccgt ccggttggcg
      541 caaatggcct gcatgttctg cataatcgct cgttcacttt cataatccag cgcgctggtg
      601 gcttcgtcaa aaatcagaat gcgcggattg gtgattaacg cgcgggcaat cgcaatacgc
      661 tgccgctgtc cgcccgacaa gccggcccct tgttcgccca ccacggtgcc gtagccttcc
      721 ggcagctcca taataaactc gtgtgcgccc gccagtttgg ctgcttcgat aatgcgttcc
      781 agcggcatac ccgtatccgt cagcgcgata ttgtcgcgta tgctgcggtt gagcagcaca
      841 ttctcctgca agaccacgcc gacctgccgc cgcagccagg caggagcggc caaagccaaa
      901 tcgttgccgt ccaccaacac ccgtccctgc tccggtacat acagacgctg caccaatttg
      961 gtgagtgtgg atttgcccga ccccgaacgt cccacaatcc ccagcacttc ccccgcccga
     1021 atccgcaggt tcaaatcctg caaaatcagc ctgccgtccg ccttatagcg gaaatcgaca
     1081 tgttcgaacg taatctcccc ccggatatcg ggcaaagcca aatgcgaaga cgcattctcg
     1141 gtcggcgcat tcagaatatc ccccaaacgc gccaccgaaa tccccacctg ctggaaatcc
     1201 tgccacaact gcgccaaacg gataacaggc gccgccacct gtcccgagag catattaaac
     1261 gcaatcagct gccccaccgt cagcttgccc tcaattacca gccgtgcgcc aatccacaac
     1321 gtcgccaccg tcaccagctt ctgaatcagc tgcaccccct gctggccgac caccgccaac
     1381 ttcgttaccc gaaatcccga agccacataa gccgccaact gattgtccca acgctgcgtc
     1441 atctgcggct ccaccgccat cgcctttacc gtacccaccg cagtgatgct ttctactaaa
     1501 aacgactggt tgtctgcatt gcgcgcgaac ttatcgttca gacgcgtccg cagtatcgga
     1561 ctgataaatg ccgaccaaaa cgcataggca ggcaacgaag ccaataccac ccaagtcaga
     1621 gtggagctgt aataccacat caccgccaga aagataaacg aaaacgccaa atccaacacc
     1681 gaagtcagcg cctgaccggt caagaaattg cgaatctgct ccaattcccg cacccgagcc
     1741 accgtatcac ccactcgtct gtgctcgaaa taggataaag gcagggaaag cagatgccgg
     1801 aacaaacgcg cgcccaattc cacatcaata cgtgaagtcg tatgtgcaaa cagatacgtc
     1861 cgcaaaccgc ccaacacaat ctcaaacagc gacaccacca acaaagccac cgacaccaca
     1921 tccaaagtag agaatccccg atgtaccagc accttgtcca tcaccacttg gaaaaacaga
     1981 ggcgtaatca gcgcaaacag ctgcaacacc accgacacca ccaatacttc aaaaaacaac
     2041 cggcggtatt tgattaccgc cggaataaac caggtaaagt caaactttgc caaactgccc
     2101 aataccgaag cgcgggaagc aaccaatatc agtttgcccg aatatctgtt agaaaattcg
     2161 gcaaaagaca ataccgcaga cttattcgta accaaatcct gtatcaaaaa ttgggcatgc
     2221 tcaccctcac cgtctgtttt ggccaaaatg aaatggttgc cgtcatcaca ccataccaat
     2281 gcgggtaaag tcgccatagc caaacgttta ataggctggc ggactacctt tgccttcaat
     2341 cccaaagatt tggcggctaa cagccattgc gtttcattta aatcgctctg tgcggaagta
     2401 caaaattcat gctgtatatc ggcaggattg gcggcaatgc cgtggtaatg ggcgaggatg
     2461 atgagggcgg aaagggcggg gagcggtgcg gatacgatag acataaataa aatatagtta
     2521 gattggatgt ggataacggc tggctggaaa aggaatatat taagtagaaa gaaatatata
     2581 aataaaacag cagaacgcat tgtaaggata tatatgggaa ttgtaaagag aaagtatgga
     2641 aaagttctcg tttcaggaag gtaaaacggc ttaggaatcg agttagatga ggatgcctcg
     2701 cacctctcgt gcctcctgca taccgttaag gaacagggtt aaggtgcagg ctgctccgaa
     2761 ctctgttgcg gtcggataat gttatttttt gtgtttcagt cagcctgaaa tatctgtata
     2821 tttttgtttt aaatagattt taaagattga taactgttct tgacgatttt tcaagaaagg
     2881 agtaaagtga cttattatca atgacaagca acgcgcgaag tgacaaggaa aactatctac
     2941 ttaaattcta aggaggcttc gaatatcata aaccaatcag aaacatagag ataaaaatta
     3001 tgtacaaata taatcctctt atacaattta ttgcacagtt gattatgtct tatggagcaa
     3061 gcatagggtg ggcacttgct gcccacgcgt ttcatatttc aaggcagcct gaaactgtgt
     3121 gggcataatg cctaccctac atcccaaaaa aacaagcgca gcctgcgtgt gtagggtgcg
     3181 aactttcggc aggcagacac gcggttttat agtttcaagc tgagttggga tgcttaagat
     3241 aaagtacaaa accttaaaaa ataagggggc tgtactagat tagccctaaa ttccacacca
     3301 atcccgcaaa atttttagct gtcgggacgg tgtgccgaag ttaaaccgaa atttgcattc
     3361 tttcaagaac agcgggaaag atttacgatc aatttcgttg tatttgcgca aggcgcgttt
     3421 cgcctgattc caaaagtttt caatgccgtt aatgtggttc tgacggtctg caaactcctt
     3481 agaatggttg atgcggtgat ggataaaacc gcttacgtgc agcttgtcat agctgctcag
     3541 actatcggta taaacaatac tgtccggcat gattttcctt ttgatgacag ggagtaacgt
     3601 ttcagacctg gcattatcta caacaacggt atagacccgt ccgttgcgtt tcagaatacc
     3661 gaagacaacc acttttcctg ccgcaccgcg accgcgtctg cctttacgcc gtccgccgaa
     3721 atagctttcg tccaactcga cagagccctc aaaaacctca tcggcagcca aggccggata
     3781 atggctgatg accatacgga ttgtgcggta gaacagaata gcggagtcgg gctggatgcc
     3841 caaaatatcg gcagctgaac gggcggtaac ttcaagtaca aaaaagcgga gcagttcttt
     3901 ctgtactttt ttctttaatt tgcagtgtgt tgtcttcata tttcgagggt aacatatctg
     3961 ctaatctagt acagcctcaa acaaaaaaga gaaattttaa tttcgctaaa tcgcataaag
     4021 attaatcaag agtatcatta aatgatatga gtgagcatct ataatgccaa gaagagttgt
     4081 taagacataa tgattattga aatagattgt aaaatagata cttagatagt ctgaaaaacg
     4141 gatttgtgaa actttttatt acgcgccatc atttgaaaat gaaacttaaa aaacacttat
     4201 cataataaat attttcttta cgttgtttgc taataaactc agtgcaatat catcgcaata
     4261 ttttatggaa attttatgga taacaaaaaa gaatttatta ataatttaac aaataggtat
     4321 atgtggatct atccattggt cttaaatatt ctatttctac ctttttacca gccctaccaa
     4381 tcttttttta ttgcgcttgg ttgtttgttt gcactggtta gaaaaatgca aagcttagat
     4441 tttaaattac aaaatcatat tgtattgtta aatataaaaa gtgcttgggc agataaaaaa
     4501 gtatttttga ttaggatagt agtgtcatgg ttggcggtaa tggaaatatg gatgtgtttt
     4561 atttcggaat catcaacgtg ggtatgcggt gctttttgtt taaatagtga aatattggaa
     4621 aaaatttttc gtggctttgg ttattctggt agtttatatt ttttatttat attgatgatt
     4681 gatctcaata agttaagaga gagtatttga atttcatttt ttgtttgact taaactcaag
     4741 gagaataaca atgattggta gtggtgatac taaacaatgc aaaaaatttt ctgcggatga
     4801 gtttgcaaaa acaatccaaa gcaactttca atcaggtatt aaatatgatg ggccgacttt
     4861 ggcaacggca ggcgtatcta atataataga tgaatactta aaggatgctt atggcggaaa
     4921 tgggttaaaa gaagcgcaac gcaatcttgt agctaaagag attgcggata gaatagaggc
     4981 agaattgcat aggcagcgtg ctaattatca agcacaagaa gctgcaaaat attctcctgc
     5041 gcatggcgga gtggagaaaa gtatgtacaa gcatcatgct gatcttcaaa aaacaaatac
     5101 taatattgct aatcgttatt ctgcaagtgc taatgaatat gcggctcaag caaaatcatt
     5161 aggaagtttg ccaaaatcag ttggctatgc tgcactaaaa aatttgggaa atgctttggc
     5221 ggcgttgcaa atgggagaag ctctatactc aaataaagct aataatatag ccaaaacagg
     5281 ctcttctatt ttaggtggta tgttagcggt tgcagcagca ggtgctatag ctagtttagc
     5341 aggagttaca ttgccttttt gggcttctat tgcagttggt gcatctattg gtttaggtat
     5401 tagtgcagca tgggatgctt ttggtgttgg ggaaaaatta ggattagacc ctaatgtgcc
     5461 agcttttgat ccattcttcg gttggctata cgataaaaca ggcggctggg aaggaggtat
     5521 tcctgattgg gctaaagaat ggttgggatt aaaccgcgat ggcaaatacc accaatacga
     5581 cccactaacc ctagacttag acggagacgg catagaaacc
//
LOCUS       DAWKQJ010000127         5423 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000127 DAWKQJ010000000
VERSION     DAWKQJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.127"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            211..2385
                     /locus_tag="WJL20_001613"
     CDS             211..2385
                     /locus_tag="WJL20_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7683747.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRNLHPTAMHKENEVKLNESDWEATGLPGDPKNL
                     PERQKSVIEKVKTDDGSNIYSSPYLTQSNHQNGSAGNGANQPKNEVEDYKDFKYVYSG
                     WFYKHAAKEIDSDKKKFKLGDDGYIFYHGKEPSRQLPASGKVTYKGVWHFATDVKKSQ
                     KFRDIIQSSKGQGDKYSGFSGDEGEEYSNKNEATLKSGHEGYGFTSNLEVDFGNKKLT
                     GKLIRNNASLNNNTNNDKHTTQYYSLEAQVTGNRFSGTATATDKKENEAQHPFVSDSS
                     SLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKAANGNTAAASGGTDAAASNGAA
                     GTSSENSKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPETSESGSNQA
                     DKGKKGKNGKNGGTDFTYKTTYTPKSDEKDTQAQTGAGGAQAASGTESVNGGQAGTKT
                     YEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKE
                     IPNEQNVVYRGSWYGHIANNTSTSWSGNASNATSGNRAEFTVNFGDKKITGTLTANDR
                     TQPTFTITANIKDNGFEGTAKTADLGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEE
                     LGGWFAYPGDKQTEKATDASGNGNSASSATVVFGAKRQKPVQ"
     gene            2471..5221
                     /gene="tbpA"
                     /locus_tag="WJL20_001614"
     CDS             2471..5221
                     /gene="tbpA"
                     /locus_tag="WJL20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ7683748.1"
                     /translation="MQQQHLFRLNILCLSLMTALPAYAENVQAGQSQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDSKYAYFIVEEECESKNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYYQHANRAYSSNTPPKNGNGGSNPNPNGDKDKPYWVSIGGGNVVTGQICLFG
                     NNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRT
                     LSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAG
                     IVFKGDFGNLEASWFNNAYRDLIVRGYEAQIENGKETAKGDPAYLNAQSARITGINVL
                     GKIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGY
                     DQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYT
                     IKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEM
                     KF"
BASE COUNT         1586 a         1340 c         1376 g         1121 t
ORIGIN      
        1 aacgcactgg attcccgcct gcgcgggaat gacgggatat acgtgggaat gacgggatat
       61 aggcttccgt gcggacgcgt tcggattcac gactgcgcgg gaatgacggg attttggtgt
      121 attccctaaa aaaataaaaa agtatttgca aatttgttaa aaataaataa aataataatc
      181 cttatcattc tttaattgaa ttgggtttat atgaacaatc cattggtgaa tcaggctgct
      241 atggtgctgc ctgtgttttt gttgagtgct tgtttgggcg gaggcggcag tttcgatctt
      301 gattctgtcg ataccgaagc cccgcgtccc gcgccaaagt atcaagatgt ttcttccgaa
      361 aagccgaaag cccaaaaaga ccaaggcgga tacggttttg caatgcgctt caagcggaga
      421 aatttgcatc ctacggcaat gcataaagaa aatgaggtta aactgaacga gagtgattgg
      481 gaggcgacag gattgccagg cgatcccaaa aacttacccg agcgacagaa atcggttatt
      541 gaaaaagtaa aaacagacga cggcagcaat atttattctt ccccctatct cacgcaatca
      601 aaccatcaaa acggcagcgc cggcaacggt gcaaaccaac caaaaaatga agtagaagat
      661 tataaagatt tcaaatatgt ttattccggc tggttttata aacatgcagc gaaagaaata
      721 gattccgata aaaaaaaatt taagttaggc gacgacggtt atatctttta tcacggcaaa
      781 gaaccttccc gacaacttcc cgcttctgga aaagttacct ataaaggtgt gtggcatttt
      841 gcgaccgatg tcaaaaaatc ccaaaaattt cgcgatatta tccagtcttc aaaaggacaa
      901 ggcgacaagt atagcggatt ttcgggcgat gaaggcgaag aatattccaa taaaaatgaa
      961 gcgactttaa aaagcggtca tgaaggttat ggttttacct cgaatttaga agtggatttc
     1021 ggcaataaga aattgacggg taaattaata cgcaataatg cgagcctaaa taataatact
     1081 aataatgaca agcacaccac ccaatactac agccttgagg ctcaagtaac aggcaaccgc
     1141 ttcagcggca cggcaacggc aactgacaaa aaagagaatg aagcccaaca tccttttgtt
     1201 tccgactcgt cttctttgag cggcggcttt ttcggcccgc agggtgagga attgggtttc
     1261 cgctttttga gcaacgatca aaaagttgcc gttgtcggca gcgcgaaaac ccaagacaaa
     1321 gccgcaaatg gcaatactgc ggcggcttca ggcggcacag atgcggcagc atcaaacggt
     1381 gcggcaggca cgtcgtctga aaacagtaag ctgaccacgg ttttggatgc ggttgaattg
     1441 acactaaacg acaagaaaat caaaaatctc gacaacttca gcaatgccgc ccaactggtt
     1501 gtcgacggca ttatgattcc gctcctgccc gagacttccg aaagtgggag caatcaggca
     1561 gataaaggta aaaaaggtaa aaacggtaaa aacggcggaa cagactttac ctacaaaaca
     1621 acctacacgc cgaaaagcga tgaaaaagac acccaagccc aaacaggcgc gggcggcgcg
     1681 caagctgctt cgggtactga gagcgttaac ggcgggcagg caggaacgaa aacctatgaa
     1741 gttgaagtct gctgttccaa cctcaattat ctgaaatacg gaatgttgac gcgtaaaaac
     1801 agcaagtccg cgatgcaggc aggaggaaac agtagtcaag ctgatgctaa aacggaacaa
     1861 gttgaacaaa gtatgttcct ccaaggcgag cgcaccgatg aaaaagagat tccaaacgag
     1921 caaaacgtcg tttatcgggg gtcttggtac gggcatattg ccaacaacac aagcacaagc
     1981 tggagcggca atgcttccaa tgcaacgagt ggcaacaggg cggaatttac tgtgaatttt
     2041 ggcgataaaa aaattaccgg cacgttaacc gctaatgata ggactcagcc aacctttact
     2101 atcacggcta atattaagga caacggcttt gaaggtacgg caaaaactgc tgacttaggt
     2161 tttgatctcg atcaaagcaa taccaccggc acgcctaagg catatatcac agatgccaag
     2221 gtgcagggcg gtttttacgg gcccaaagcc gaagagttgg gcggatggtt tgcctatccg
     2281 ggcgataaac aaacggaaaa ggcaacagat gcatccggca atggaaattc agcaagcagt
     2341 gcaactgtcg tattcggtgc gaaacgccaa aagcctgtgc aataagcacg gttgccgaac
     2401 aatcaagaat aaggcttcga cggcatcgct ccttccgatg ccgtctgaaa gcgaagatta
     2461 gggaaacact atgcaacagc aacatttgtt ccgattaaat attttatgcc tgtctttaat
     2521 gaccgcgctg cctgcttatg cagaaaatgt gcaagccgga caatcacagg aaaaacagtt
     2581 ggataccata caggtaaaag ccaaaaaaca gaaaacccgc cgcgataacg aagtaaccgg
     2641 tttgggcaaa ttggtcaaaa ccgccgacac cctcagcaag gaacaggtac tcgatatccg
     2701 cgacctgacg cgttacgacc ccggcatcgc cgtggtcgaa caggggcgcg gcgcaagctc
     2761 tggctactcg atacgcggta tggacaaaaa ccgcgtttcc ttgacggtgg acggcttggc
     2821 gcaaatacag tcctacaccg cgcaggcggc attgggcggg acgaggacgg cgggcagcag
     2881 cggcgcaatc aatgaaatcg agtatgaaaa cgtcaaagct gtcgaaatca gcaaaggctc
     2941 aaactcggtc gaacaaggca gcggcgcatt ggcgggctcg gtcgcatttc aaaccaaaac
     3001 cgccgacgat gttatcgggg aaggcaggca gtggggcatt cagagtaaaa ccgcctattc
     3061 cggcaaaaac cgggggctta cccaatccat cgcgctggcg gggcgcatcg gcggtgcgga
     3121 ggctttgctg atccacacgg gtcggcgcgc gggggaaatc cgcgcacacg aagatgccgg
     3181 acgcggcgtt cagagcttta acaggctggt gccggttgaa gacgacagca aatacgccta
     3241 tttcatcgtt gaagaagaat gcgaaagcaa aaattacgaa acgtgtaaag cgaatccgaa
     3301 aaaagatgtt gtcggcaaag acgaacgtca aacggtttcc accagagact acacgggtcc
     3361 caaccgcttc ctcgccgatc cgctttcata cgaaagccgg tcgtggctgt tccgcccggg
     3421 ttttcgtttt gagaataagc ggcactacat cggcggcata ctcgaacaca cgcaacaaac
     3481 tttcgacacg cgcgatatga cggttccggc attcctgacc aaggcggttt ttgatgaaaa
     3541 tagaaaatac ggaccgataa gaggcaacgg caaatacgcg ggcaaccaca aatacggcgg
     3601 actgtttacc aacggcgaaa atggtgcgct ggtgggcgcg gaatacggta cgggcgtgtt
     3661 ttacgacgag acgcacacca aaagccgcta cggtttggaa tatgtctata ccaatgccga
     3721 taaagacact tgggcggatt atgcccgcct ctcttacgac cggcagggca tcggtttgga
     3781 caaccatttt cagcagacgc actgttctgc cgacggttcg gacaaatatt gccgtccgag
     3841 tgccgacaag ccgttttcct attacaaatc cgatcgcgtg atttacgggg aaagccacag
     3901 gctcttgcag gcggcattca aaaaatcctt cgataccgcc aaaatccgcc acaacctgag
     3961 cgtgaatctc gggtttgacc gctttggctc taatctccgc catcaggatt attattatca
     4021 acatgccaac cgcgcctatt cgtcgaacac gccccctaaa aacggcaacg gcggctcaaa
     4081 ccccaacccc aacggcgaca aggacaaacc ctattgggtc agcataggcg ggggaaatgt
     4141 ggttacgggg caaatctgcc tctttggcaa caatacttat acggactgca cgccgcgcag
     4201 catcaacggc aaaagctatt acgcggcagt tcgggacaat gtccgtttgg gcaggtgggc
     4261 ggatgtcggc gcggggttgc gctacgacta ccgcagcacg cattcggacg acagcagcgt
     4321 ttccaccggc acgcaccgca ccctgtcctg gaacgcaggc atcgtcctca aacctgccga
     4381 ctggctggat ttgacttacc gcacttcaac cggcttccgc ctgccctcgt ttgcggaaat
     4441 gtacggctgg cgggcgggtg ttcaaagcaa ggcggtcaaa atcgatccgg aaaaatcgtt
     4501 caacaaagaa gccggcatcg tgtttaaagg cgatttcggc aacttggagg caagttggtt
     4561 caacaatgcc taccgcgatt tgattgtccg gggttatgaa gcgcaaattg aaaacggcaa
     4621 agaaacagcc aaaggcgacc cggcttacct caatgcccaa agcgcgcgga ttaccggcat
     4681 caatgttttg ggcaaaatcg attggaacgg cgtatgggat aaattgcccg aaggttggta
     4741 ttctacattt gcctataatc gtgtccatgt ccgcgacatc aaaaaacgcg cagaccgcac
     4801 cgatattcaa tcacatctgt ttgatgccat ccaaccctcg cgctatgtcg tcggcttggg
     4861 ctatgaccaa ccggaaggca aatggggtgt gaacggtatg ctgacttatt ccaaagccaa
     4921 ggaaatcaca gagttgttgg gcagccgggc tttgctcaac ggcaacagcc gcaatacaaa
     4981 agccaccgcg cgccgtaccc gcccttggta tattgtggat gtgtccggtt attacacgat
     5041 taaaaaacac ttcaccctcc gtgcgggcgt gtacaacctc ctcaactacc gctatgttac
     5101 ttgggaaaat gtgcggcaaa ctgccggcgg cgcagtcaac caacacaaaa atgtcggcgt
     5161 ttacaaccga tatgccgccc ccggtcgcaa ctacacattt agcttggaaa tgaagtttta
     5221 aacgtccaaa cgccgcaaat gccgtctgaa aggcttcaga cggcattttt tacataattc
     5281 ccaccgtttc ccatcatccc cgatacaccg taatcctgaa atccgtcatt cccgagcagg
     5341 cgggaatcca gtccgttcgg tttaggtttt tttgaggttt cgggtaactc taaaccgtca
     5401 ttcctacgaa aacagaaaat caa
//
LOCUS       DAWKQJ010000128         5321 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000128 DAWKQJ010000000
VERSION     DAWKQJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.128"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            295..1728
                     /locus_tag="WJL20_001615"
     CDS             295..1728
                     /locus_tag="WJL20_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ7683749.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGETLAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLIKENKEKLARIMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALLKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1837..2625
                     /locus_tag="WJL20_001616"
     CDS             1837..2625
                     /locus_tag="WJL20_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ7683750.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="WJL20_001617"
     CDS             2993..4549
                     /locus_tag="WJL20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7683751.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGDFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="WJL20_001618"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="WJL20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ7683752.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKTPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1238 a         1341 c         1522 g         1220 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tattaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cgctgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattacc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacggcatc
      361 ggggtcggca atccggcgac gggcgagact ttggcgtatg tccgcaatac gggttcggac
      421 aaactgaaaa acctgattca aaaagcagct gcggcacaaa aattatgggc ggcaaaaacc
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tgattaaaga aaacaaagaa
      541 aaactggcgc ggatcatgac gatggagcag ggtaaaagcc tgactgagtc gcgtggtgaa
      601 atcgattatg cggcttcgtt tgtgcgctgg tttgccgaag aagcgcggcg gattgacggc
      661 gatgtgctga cgagtgtgaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgctg cgattacgcc gtggaacttc ccatctgcaa tgattgcgcg caaggctgcg
      781 cctgctttgg cggtgggctg cgcgatgatc gtcaaacccg catcgctcac gcctttaagc
      841 gcgtatgcgt tggtcgtgct ggcttatgaa gcgggcgtgc cgcaggattt gctgcccgtc
      901 gtcagcggca gtgcatcgga aatcggccgt gaatttgcca cgaacccgat tatccgcaaa
      961 atcagcttca ccggctctac cgaagtcggt gcgaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga gctgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggtgcgctc gccagcaagt tccgcaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgcccaa tcgggcattt acgacgcatt ttgccgcaaa
     1201 ttgagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg cgtaaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagttgagc agcacatcgc cgacgcgctc
     1321 ttaaaaggcg cggtctgtct gaccggcggc aaacgcagcg cgttgggtgg aacgtttttt
     1381 gagccgaccg tcttaagcgg cgtaacggcg caaatggcgg tagctcgcga ggaaaccttc
     1441 gggcctttgt gtccggtatt ccgttttgaa accgaagccg aggtcatcga ggctgcgaac
     1501 aatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag ccttggaata cggcatggtt ggcatcaata cgggcttaat cagtaatgaa
     1621 gcggcaccgt ttggcggcgt gaaacgcagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctggaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg gtttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcatc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt ttctttggcg
     2161 cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc cgccgaagtg
     2221 gcggttgaag atttgcacaa agcgccggaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag
     2401 gaagcagaaa aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcgt tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 cgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggcggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttactgttg ccgacaacca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaac tgtttaacca gatttcccag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaaacggac tgctgaaaat gtcttgggta tgatgggcat
     3781 acgcgaaagc gtgcttgccg acgcgggcgg cgaatccggg gacgacgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagta
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggaaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc gctcgtccta cccgacggca cgaccgcctt
     4141 gggcgcgctc gaactcttcc gccaaagcag cgcggattac gccctcgtcg tggacgagtt
     4201 cggcgcggtg ttgggtatgg tgacgatgaa agacctgttg gaagccattg ccggcgactt
     4261 tcccgaagaa ttcgaacgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga gctggcatcc caactcaatc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggtgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag tattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc atcgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt cgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acgctggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 ggttctacta tgtcaacggc acgccgaccg actgcatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatacccg
     4921 ccgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcgaccgca gaacaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa cccccccgca gtcccctatt ttatggaaca
     5041 tcaatatccc cgccgtcgcg ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggagacacca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggatagggcc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacagc ggcgttctgg catgcggact gaccgtttca t
//
LOCUS       DAWKQJ010000129         5297 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000129 DAWKQJ010000000
VERSION     DAWKQJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.129"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            157..2361
                     /locus_tag="WJL20_001619"
     CDS             157..2361
                     /locus_tag="WJL20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7683753.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGSSVETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVKYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKTLTGQLIKNQYVQKKTD
                     EKKPLTIYNITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKKTETANAADTKPALPSGKHTKILDSLKISVDEATDGH
                     ARKFAISSMPDFGHPDKLLVEGREIPLVNKEQIIKLADGRKMTVSACCDFLTYVKLGR
                     IKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDEDEE
                     EEPEETEETEEKSPTEGNGGSGSILPALEAPKGRDIDLFLKGIRTAEADIPQTGKARY
                     TGTWEARIGEPIQWDNQADKKEAKAEFDVDFGEKSISGKLTEKNGVQPAFHIENGKIE
                     GNGFHATARTRDNGINLSGTGSTNPKTFQANDLRVEGGFYGPQAAELGGIIFNNDGKS
                     LDITEDTENKVDVEAEVDVDAEADVGKQLESEVKHQFGVVFGAKKDNKEVEK"
     gene            2358..5195
                     /gene="lbpA"
                     /locus_tag="WJL20_001620"
                     /gene_synonym="tbpA"
     CDS             2358..5195
                     /gene="lbpA"
                     /locus_tag="WJL20_001620"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ7683754.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSKDKTGQFFSVEGERES
                     KPVAKLAGYGKYLNNQLNRWVKERIEKKQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEHT
                     GEYTADDKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKY
                     FDFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSF
                     QELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVA
                     DHKTKLPDSAGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYN
                     RIKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDEL
                     AYLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTA
                     ESTANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1512 a         1236 c         1414 g         1135 t
ORIGIN      
        1 atgtaggttc gtgggaatga cggaatgtat ctgtactgtc tgcggctcgt cgccttgtcc
       61 tgatttttgt taattcacta taaaaacggg ttgatattat ctgtacatat taatataatg
      121 ataattatta ttaatcaaat aggaggaaaa gtagggatgt gtaaaccgaa ttatggcggc
      181 attgtcttgt tgcccttact tttggcatct tgtatcggcg gcaatttcgg cgtgcagcct
      241 gttgtcgaat caacgccgac cgcgtacccc gtcactttca agtctaagga cgttcccact
      301 tcgccccctg ccgggtcttc ggtagaaacc acgccggtca atcagcccgc cgtcggtgcg
      361 gcaatgcggc tgccaaggcg gaatatcgca gcttttgata aaaatggtaa tgaaattccc
      421 gatagtaagc aggcagagga gtatctgccg ctcaaagagg aggatatcct gtttttagac
      481 ggtacgccga aagaacaggc tgacaaactt aaaaaggaaa tcaacggacg gtatcctaat
      541 gtaacaatct acacgtccga tttaaaagat gctgtgtata aatatgaaca tgtccgggcc
      601 ggatatgttt atactagatt tggaacagat gaaatcgaac agaactcagg tggtaagcag
      661 gttacccacc gcttaggtta tgatggtttt gtatattatt ctggagaacg tccttcccaa
      721 tctttaccga gcgcgggaac ggtgaaatat tccggaaact ggcaatatat gaccgatgtc
      781 aaacgtcatc gagcaggtaa ggcggttggc attgacaatt tgggttatat cacattttat
      841 ggtaacgatg ttggtgcaac ttcttatgcg gctaaggatg tcgacgaaag ggaaaaacat
      901 cctgctaaat atacggttga tttcgataag aaaactttga caggtcaatt aattaaaaat
      961 cagtatgtgc aaaagaaaac cgatgaaaag aaaccactga ccatttacaa cattactgca
     1021 aaattggacg gcaaccgctt taccggcagt gccaaggtca atcctgattt agcgaaaaac
     1081 cctgccggta atgagcgttt gtttttccat gccgatgccg atcagcggct tgagggcggt
     1141 tttttcggcg ataacggaga agagcttgcc ggacggttta tcagcaacga caacagcgta
     1201 ttcggcgtat tcgcaggcaa aaaaacagag acagcaaacg cagcagatac aaaacctgcc
     1261 ctgccgtctg gaaaacacac caaaatcttg gattctctaa aaatttccgt tgacgaggcg
     1321 actgatggcc atgcccgtaa gtttgccatt tcctctatgc ccgattttgg tcatcccgac
     1381 aaacttcttg tcgaagggcg tgaaattcct ttggtaaaca aagaacaaat catcaagctt
     1441 gccgacggca ggaaaatgac cgtcagtgct tgttgcgatt ttctgaccta tgtgaaactc
     1501 ggacggataa aaaccgaacg ccccgccgtc aaaccgaagg cgcaggatga agaggattcg
     1561 gacattgata atggcgaaga aagcgaagac gaaatttccg aagatgataa cggcgaagat
     1621 gaagtcaccg aagaagagga agctgaagaa accgaagaag aaactgatga agacgaagag
     1681 gaagaacccg aagaaactga agaaactgaa gaaaaatcgc cgacagaagg caacggcggt
     1741 tcaggcagca tcctgcctgc cctagaagcc cctaaaggca gggacatcga ccttttcctg
     1801 aaaggtatcc gcacggcgga agccgacatt ccgcaaaccg gaaaagcacg ctataccggc
     1861 acttgggaag cgcgtatcgg cgaacccatt caatgggaca atcaggcgga taaaaaagag
     1921 gcaaaagcag aatttgacgt tgatttcggc gagaaatcga tttccggaaa gctgacggag
     1981 aaaaacggtg tacaacctgc tttccatatt gaaaacggca agattgaggg caacggtttc
     2041 cacgcgacag cgcgcactcg ggataacggc atcaatcttt cgggaactgg ttcgaccaac
     2101 cccaaaacct tccaagctaa tgatcttcgt gtagaaggag gattttacgg cccgcaggcg
     2161 gcggaattgg gcggtattat tttcaataat gatgggaaat ctcttgatat aactgaagat
     2221 actgaaaata aagttgatgt tgaagctgaa gttgatgttg atgctgaagc tgatgttggc
     2281 aaacagttag aatctgaagt taaacaccaa ttcggcgtgg tattcggtgc gaagaaagat
     2341 aataaagagg tggaaaaatg aataagaaac acggttttca gcttaccctg actgccttgg
     2401 ctgttgccgc cgccttcccg tcatatgcgg caaaccctga aacggcggca cctgatgcgg
     2461 cgcagaccca atccctgaaa gaggttaccg tccgtgccgc caaagtggga cggcgatcga
     2521 aagaggcgac cggtttgggc aaaatcgtca aaacgtcgga aacgttgaac aaagaacagg
     2581 tactcggtat ccgcgacctg acgcgctacg atccgggcgt ggcggttgtc gaacagggca
     2641 acggcgcgag cggcggctac tcgatacgcg gcgtggataa aaaccgtgtg gcggtttcgg
     2701 ttgacggcgt tgcccaaata caggcgttta ccgtgcaggg atcgttgagc ggatacggcg
     2761 gacgcggcgg cagcggtgca atcaacgaaa tcgaatatga aaacatcagc acggtggaaa
     2821 tcgacaaagg tgccggttcg tccgatcacg gcagcggcgc actcggcggc gcggtcgcct
     2881 tccgcaccaa agaggcggca gacctgattt cagacggcaa aagctggggg atacaggcaa
     2941 aaaccgccta cggcagtaaa aaccgccaat ttatgaagtc gctcggcgcg gggttcagca
     3001 aagacggttg ggaagggctg ctaatccgaa ccgaacgcca agggcgggaa acgcgcccgc
     3061 acggcgatat tgcggacggg gtggcatacg gcataaaccg tttggatgcg ttccgccaga
     3121 cacacgatat tcaaagcaaa gataaaacag ggcaattttt ctcagtagag ggcgagcgtg
     3181 aatccaagcc cgtggcaaaa ttggcgggct acgggaaata tttgaacaac cagctcaacc
     3241 gctgggtaaa agaacgtatt gaaaaaaaac agcctttaag tgctgaagaa gaagcgatgg
     3301 tgcgggaggc gcaggcgcgc cacgaaaacc tgtccgccca agcttacacg ggcggcggca
     3361 ggatattgcc cgatccgatg gattaccgca gcggctcttg gcttgccaag ctgggctacc
     3421 gcttcggcgg caggcattat gtcggcggcg tgtttgagga taccaaacag cgttacgaca
     3481 tccgcgatat gacggaaaaa cagtattacg gtacggacga ggcggacaag tttagagaca
     3541 agagtggggt gtacgacggc gacgatttcc gcgacggctt gtattttgtg ccgaatatag
     3601 aagagtggaa gggcgataaa aagttggtca agggcatagg tttgaaatat tcccgcacca
     3661 aatttattga cgaacatcac cgccgccgcc gtatgggttt gctgtaccgt tatgaaaacg
     3721 aagcgtattc tgacaattgg gcggacaagg cggtgttgtc gtttgacaaa cagggcgtgg
     3781 caaccgacaa caacacgctg aagctgaatt gcgccgtgta tcctgccgtg gacaaatcct
     3841 gccgcgcgtc ggcggacaaa ccgtattcct acgacagcag cgaccgtttc cactaccgcg
     3901 aacagcacaa tgttttgaat gcctcgtttg agaaatcgct gaaaaacaaa tggacgaaac
     3961 accatctgac tttgggcttc ggttacgatg cttccaacgc gatctcccgc cctgaacagc
     4021 tttcccacaa tgcggcaagg atttcggaac acacggggga gtacaccgcc gacgacaaag
     4081 acaagtaccg tttgggtaag cccgaagtcg tcgaagggtc ggtctgcggc tacatcgaaa
     4141 ccctgcgttc ccgcaaatgc gtgccaagaa aaatcaatgg cagcaatatc cacatttctt
     4201 tgaacgaccg tttttcaatc ggcaaatatt ttgatttcag cttgggtggc aggtacgacc
     4261 ggaaaaactt caccacgtcg gaagaactcg tccgcagcgg acggtatgtt gaccgttcgt
     4321 ggaacagcgg catcgtgttc aaaccgaacc ggcatttttc tttgtcttac cgcgcctcca
     4381 gcggcttcag aacgccctca ttccaagaac ttttcgggat agacatttat cacgattatc
     4441 cgaaaggctg gcagcgtccc gccctgaaat cggaaaaggc agccaaccgc gaaatcggtt
     4501 tgcagtggaa gggcgatttc ggctttttgg aaatcagcag cttccgcaac cgttataccg
     4561 atatgattgc cgttgccgat cacaaaacca aattgccgga ttcagcagga cgattgacag
     4621 agattgatat acgcgattat tacaatgccc aaaatatgtc gcttcaaggc gttaatatat
     4681 tgggaaaaat cgactggaac ggcgtgtatg gcaaactgcc cgaaggtttg tacacgactt
     4741 tggcgtacaa ccgcatcaaa ccgaaatcgg tatccaaccg gccgggactg tctctccgca
     4801 gctatgcttt ggatgcggta cagccgtcgc gttatgtttt ggggttcgga tacgaccagc
     4861 cagaggggaa atggggcgca aacattatgc tgacctattc caaagggaaa aaccctgacg
     4921 agcttgctta tctggcaggc gatcaaaaac gatattcgac aaaaagagcg tcgtcttctt
     4981 ggtcgacggc agacgtttcc gcctatctga atctgaaaaa acggctgacc ttgagggcgg
     5041 ctatctacaa tatcggcaac taccgctacg ttacttggga atccttgcgc cagactgcgg
     5101 aaagcacggc aaaccggcac ggcggcgaca gcaactatgg aaggtatgcc gcaccgggca
     5161 ggaacttcag cctcgcgctc gaaatgaagt tttaaaggaa ataccgtctg aaagcttgat
     5221 ctgcacccca aaagtcggac taaaccgcca actgattaag gtgcaggttt ttttgattcg
     5281 atataaacaa gatttcc
//
LOCUS       DAWKQJ010000130         5282 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000130 DAWKQJ010000000
VERSION     DAWKQJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.130"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..807
                     /gene="pgl"
                     /locus_tag="WJL20_001621"
     CDS             112..807
                     /gene="pgl"
                     /locus_tag="WJL20_001621"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ7683755.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            788..1774
                     /locus_tag="WJL20_001622"
     CDS             788..1774
                     /locus_tag="WJL20_001622"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ7683756.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1824..2672
                     /gene="hexR"
                     /locus_tag="WJL20_001623"
     CDS             1824..2672
                     /gene="hexR"
                     /locus_tag="WJL20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ7683757.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2793..4439
                     /gene="pgi"
                     /locus_tag="WJL20_001624"
     CDS             2793..4439
                     /gene="pgi"
                     /locus_tag="WJL20_001624"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7683758.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIVMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4453..5004
                     /locus_tag="WJL20_001625"
     CDS             4453..5004
                     /locus_tag="WJL20_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7683759.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1340 a         1475 c         1398 g         1069 t
ORIGIN      
        1 aagcagtccc gatatttggt atcaaaagca taaacaactg ttcggccgac atattgctca
       61 acccgtttcc ataacggaat atgccgtctg aaataaaaaa aggacacaaa tatgtttgtt
      121 tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt tggctgacgc agtggcggat
      181 gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt tggcagtttc cggcggacgt
      241 tcgccgattg catttttcaa cgccctgtcg caaaaagatt tggattggaa aaacgtcggc
      301 atcaccttgg cagatgaacg catcgtgccg accgtccacg ccgacagcaa taccggtttg
      361 gtgcgcgaat acctgttgaa gaacaaagcg gaagcggcaa tgtggattcc tatggtggaa
      421 gacggaaaaa ctgaaaccga attacatccc gatgctgttg tcgattatgc actgaaacat
      481 tacaaacagc ccgatgtttt ggttttgggt atgggaaacg acgggcatac ggcttcgatt
      541 ttcccgaaag ctccgcagtt tcagacggca atcgacggtt cggcaggtgt cgcgctggtg
      601 cataccacgc ccgttaccgc gccgcacgag cgcgtcagta tgaccttgga tgcgattgcc
      661 catacggggc atgtgttttt ggcgatacgg ggcgaagaga aaaaagccgt gttcgaccaa
      721 gccgcacaag gcgaaaaccg cgaatatccg atcaacctcg ttttgaacca tcaaggagtg
      781 aactgccatg tcttctacgc cgaataaaca agccggatat ccccgactgg tcgccgatat
      841 cggcgggacg aatgcacgct ttgcgctgga aaccgcgccg cgcgtcattg aaaaagccgc
      901 cgtgcttccg tgtaaagact acgatacggt taccgatgcg gtgcgtgcct atctgaatca
      961 aagcggtgca acagccgtac ggcacgcggc atttgccatc gccaacccga ttttgggcga
     1021 ctgggtgcag atgaccaacc accattgggc gttttccatc gaaaccaccc gtcagacttt
     1081 ggggctggac accctcatcc ttttgaacga ctttaccgcg caggcattgg cggtaacgca
     1141 gacttcaagc aaagacctga tgcaggtagg cgggcaaaag cctgtcgaat ttgcccccaa
     1201 agccgttatc ggccccggta ccggcctggg cgtgagcgga ttggtgcaca gccacgcagg
     1261 ctgggtggct ttggcgggcg agggcgggca taccagtttc ccgccgtttg acgatatgga
     1321 agtgctgatt tggcagtacg ccaaaaacaa atacggccat gtttccgccg aacgcttttt
     1381 gagcggcgcg ggcttgagct tggtttacga ggctttggct gcaaaacaga aagccaaacc
     1441 cgccaaactg atgccgtctg aaatcacgga aaaggctttg agcggcgcgt cgcctttgtg
     1501 ccgtcagact ttggacatct tctgcgccat gctcggcacg gttgcttcca acctcgccct
     1561 gacgctgggc gcgcgcggcg gcgtgtacct gtgcggcggc attattcccc gcgtgttgga
     1621 atatttcaaa acttccccgt tccgcagccg tttcgagaac aagggcaggt ttgaagcata
     1681 tcttgccgcg attcccgtgt atgtcgtctt gagcgagttt cccggaattt ccggtgcggc
     1741 tgcggctctc gacaaccatt tgagaaacgt ttaaccacag cggctccttg cagcggggct
     1801 gcattatcga agggcatatc attatgttaa gcaaaatcag cgaatcactg gcaaaccttt
     1861 ccggtgcgga acgcaaagtc gccgaatgtg cattggcgga acccaaatgg ttcgtccatg
     1921 ccgccgttgc cgaaattgcc gaacgtgcgt ccgtcagcca gccgaccgtc atccgattct
     1981 gccgcagctt gggttataaa gggctgcccg agttcaagct cgccttgtcc gccagcatcg
     2041 gtcatgaggg tatgccctat gtccacgaag aactcaacgc cgacgacgat atggcaagcg
     2101 tggtcgagaa agtgttgggc aatgccgccg cctcgctgtt gggcgaacgc cgcttcctga
     2161 aagagtcgga gctggaaaac gccattgcca cgctgatgca cgcccgtcgc gtcgagtttt
     2221 acggtgtcgg caattccggc attgtggcac aggacgcgca gcataaattt ttccgtttcg
     2281 gcatgtccac cgtcgcctat gtcgatacgc acacgcagct gatggcggca tctgttttga
     2341 gcgatcagga tgttttggtt gccatttcca acacgggttc gtctatcgaa cttttggatg
     2401 cggtcagcat cgccaaagaa aacggcgcgt ctgtcatcgc actgacccgc aacgattcgc
     2461 ctctggcgca acttgccgac tgcgtgttga gcgttgccac acaggaaaat gccgaactct
     2521 acacgcccat ggtttcccgc ctcttgcagc ttgccgtcat cgacattctc gccatcggac
     2581 ttgccctgcg cttgggcgat gctgccagcc tgcaattgca gaaaagcaaa aaaagcatac
     2641 acaacaagca catcgattac gacaaagatt gaccttcaga cggcatccca caaatgccgt
     2701 ctgaaatgcc gaacaacggt cgtcggcggc ttgcggcagt ttccggcagc cttttcatcc
     2761 cacaacaaaa aacctcattc aggagcatat agatgaaaca ccttcacgac ttacccgcat
     2821 ggtcgaaatt gtggaaccac tttgacgaca gcaaaacatt gcatatgcgc gaaatgttcg
     2881 agcaagaccc gcagcgtgcg gaacgctact ggctgcaagt cggcggactg acgctggact
     2941 actccaaaaa ccgcatcaac gacgaaacca tgtcgctttt gttcgagctt gcccgagaag
     3001 caggcgtgcc ggagcggatg cggcagatgt tccacggcga aaaaatcaat accaccgaaa
     3061 accgcgccgt cctgcatgtc gcccttcgca accgcaccaa ttcgccgatt gtggttgacg
     3121 gtgaagatgt gatgcccaaa gtcaaccgcg ttttgcaacg tatgggcgaa tttgcacacg
     3181 aagtccgcag cggaagctgg ctgggctata ccaaccaagt cattaccgac gttgtcaaca
     3241 tcggcatcgg cggatcggat ttgggcccgc tgatgatgtg taccgcgctc aaacctttcg
     3301 gtcatccgcg cctcaatatg cacttcgtct ccaacgtgga cggctcgcaa ctgcgcgacg
     3361 tattgtccaa agtccacccc gaaaccacgc tgttcatcat cgcctccaaa acatttacca
     3421 cgcaggaaac gctgaccaac gcgctgaccg cgcgcgaatg gtttttgaat catgcgggcg
     3481 acgaagaagc cgttgccaaa cacttcgccg ccgtttccac caatcaaaaa gccgtcgccg
     3541 aattcggcat cgacaccgcc aatatgtttg aattttggga ttgggtcggc ggtcggtaca
     3601 gcctgtggtc cgccatcgga ttgccgatta tgctgtatct cggcgaagaa aacttcatcg
     3661 aaatgctcaa cggcgcgcac ctgatggacc aacacttcat caacacaccg ctcgagcgca
     3721 acctgcccgt cattctcgcc ctcatcggca tctggtatat caactactac ggcggcggca
     3781 gccacgtcat cgcgccttac gaccaacatt tgcaccgcct gcccaaattc atccagcagc
     3841 tcgatatgga aagcaacggc aaacaggtta cgttggacgg caaagcagtc ggacacgaaa
     3901 cctcgccgat tatctggggc gaaacgggca ttaacggcca gcacgccttc ttccaactgc
     3961 tgcaccaagg cacgcacatt acccccatcg acctgattgc ctcgcttgaa aaacgcagca
     4021 acctgcccgg acaccacgaa atcctgcttg ccaacgtctt cgcccaagca gaagccttta
     4081 tgcgcggcaa aacccccgac gaagtccgcg ccgaactcaa agcgcagggt atggatgagg
     4141 cgcgcatcga agagctggtc ccgcacaaaa ccttctccgg caaccgcccg accaacctca
     4201 ttctcatgga caaggtcaac ccgcgcaata tgggcagcct gattgtcatg tacgaacaca
     4261 aaaccttcgt acaaggcatc atttggggca tcaacagctt cgaccagtgg ggcgtggaac
     4321 tcggcaaaca actggctaaa accattttgg gcgaactgac cggcgaaacc gggccgcaaa
     4381 agcacgacag ttcgaccgaa cgcctgatca acctctacct gcagaccaac cgcaaataaa
     4441 acctgcggaa aaatgccgtc tgaacgccga ccgtttcaaa cggcattttt atcgaacagg
     4501 aaaaccgtcg gtaaacttgc agagcgtgtg caatcccgat atgatggttt gcataaattt
     4561 aaacatatat gttccgcagc tatggcactg attaaagagc cgttggacaa agcgaaacaa
     4621 aggaacgaag aacttgaagc ggcagaagaa gcggcggcgc aggaggcatt gggtcgggag
     4681 caggaagtcg accgcgtatc cgaatgggaa gaacgctaca agctgtcgcg cagcgagttc
     4741 gagcaattct ggaaaggatt gcctcaaacc gtacagaata agctgcaagc ctcacagaaa
     4801 acatggaaaa gcgggatgga taaaatctgt gccaacaatg cgaaagctga aggtgaaacg
     4861 ccaaacggca taaaattcag cgaactggca tgcaaaacgg cggaaaccga agcacgcttg
     4921 gaagagctgc acaaccgtaa aaaagccctt atcgacgaaa tggccaggga agcggacaag
     4981 aaagaactgc caaagcggct ctgaacagcg cggttcaggc actgcccgcc gatattgccg
     5041 aaaccgtcat gcccgagtac cgcaactgac aaaacggttt gaatgccaaa tgcgccgacg
     5101 gcgacgaata cggcgtggca caatctgact gccgtaccag agaaatcaat gcgaaaacca
     5161 aagaaatcca aggttatctg attgactgaa acttggatgc gggaatgtcg gcggcttttg
     5221 cgtttttgtc gtttttatag tgaattaaat ttaaaccgat acagcgttgg ctcgccttag
     5281 ct
//
LOCUS       DAWKQJ010000131         5198 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000131 DAWKQJ010000000
VERSION     DAWKQJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.131"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(232..2508)
                     /locus_tag="WJL20_001626"
     CDS             complement(232..2508)
                     /locus_tag="WJL20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7683760.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVGLETVTVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2930..3520
                     /locus_tag="WJL20_001627"
     CDS             2930..3520
                     /locus_tag="WJL20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ7683761.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3583..4641
                     /gene="trpD"
                     /locus_tag="WJL20_001628"
     CDS             3583..4641
                     /gene="trpD"
                     /locus_tag="WJL20_001628"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ7683762.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4793..5008)
                     /locus_tag="WJL20_001629"
                     /pseudo
     CDS             complement(4793..5008)
                     /locus_tag="WJL20_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1195 a         1190 c         1472 g         1341 t
ORIGIN      
        1 gaattggatt cccgcctgcg cgggaatgac gggatcttgg gtttctgctt ttgatttttc
       61 tgcttttgcg agaatgacgg cgtgaaagta agaatgatga aacaaaaaaa atgggaatga
      121 tggcatagtg gtttgttctt tgtctttgcc atatttccta acaaattgat taaaaagaaa
      181 aaaggttttc aggatgccgt ctgaaaacct tttttgtttg cctgtccgat tttaaaactt
      241 cacgttcacg ccgccggtaa agctgcggcc catttgcggc gtatcagaga gaaagctgct
      301 gtgggcgtaa acggattggt tgagcaggtt gtcggctttg acgtaccaat tccactcgcc
      361 atagcgcgta ttgcggcggt agtttgcgcc gaggttgagc atatggtgtc cgggcgtgcg
      421 cgtttcgtag cgggcgagtt tgttttgggc gaacacgcgg tagtagtcca aattggcatc
      481 gatacggtcg gtcagcgagg ctttcaggtg gaagccgagg cgcgcagccg gaacacgggg
      541 ggcattttgg tcgtcctgtg cgatgaaagg acggttgccg taggcatctt ctctgccggg
      601 tagggaaggc aggtttttca gacggcctcg tacatagtcg ccggaaacgc cgatgcggta
      661 gcgcggtgtc ggtttgaagt agatttcgcc ttccgcgccg tagaagtcgg cgccggattg
      721 gttgtagcgc acgagcttca tttcgctgtc gtcttcgatg gatttggggc cgcgtccgtc
      781 gtttaaggtt tgggcgtaaa tgtagttacc gaagcggttg cggtagagtg ccagattgta
      841 ttgccagcgg tcgccttcgt agcccagcgc gagttcgata ttgttggaac gctctttgtt
      901 gaggtgtttg ttgccgactt caaaggtgtt ggtggcgacg tgtttgccgt gtgcgtacag
      961 ctcttgcgtt gacggcaggc gttcctgatg ggaggcggtc aggctgagtt tgtgttgtgg
     1021 cgtgaaatac cagttgcccg aaagtgcgaa tgagcgggcg gtttggcggt gcgcgccgag
     1081 gtcgggcagg ggatggttgt agtagttttc ccgatcaatc aatgctttat cgtagcggat
     1141 ggaggctttt tgtttttcca cgcgtacgcc gccttcaagc gtgaagttgt cccagtttgc
     1201 ctgttctaca ccgaaaaagc tgtaatgttg cactttattg tcaagcagca tcggttgttt
     1261 gaccgcttcg gatgtggcag ataaagcact ggatttttgt tgtaaatatt gcacgcccca
     1321 gctgcctttc agacggccta tgggttggtg gcgcaactcg atgcgggcgt tttgcgtttg
     1381 gttgttaaaa aagttttcga ccgcatcgcc tgctttttcg tcgtggcggt agtcgttgcg
     1441 gttcaagtat acgcgcaggg cttcaaaacc ggggaacggt tgcttccatt cggcacggag
     1501 ttcgtagcgt ttgttgcgca ggtctatcca cggtctgccg ctgtgggtgt gtgcgtgtgc
     1561 attatcgtcg tcgtggaagc cgcagctcaa gcccggattg tcgtaatcga tgtcttcttc
     1621 ggtcaacagg tgcggataaa gctgtaaata gcgtttgtta atcaagctct tttgccagat
     1681 gatgtcggcg tggcaatcat cgtattcgtg gctgtgggca ggcagaccat attggtcgcg
     1741 acggtcgctg tacgctacgc cgataaaacc tttttcgcca acccaagaca gcccgatgct
     1801 gcccgtttgc gaatcggcgt ggctgtcggg caggcgtttc agattgcggt aacgcggtac
     1861 ggcgtaatcc cccgatttgc ggtacagccc ttccgtgtgc aatacaaagt ttttgcccaa
     1921 accgatattg atgccgccgg acgtgagttt ttccagattg ccgctgctca aacgcaatcc
     1981 gagttcgccc gatacgccgt tttcaggcat tttttcgggg attttgccat cggcaacatc
     2041 gaccagcccc gccacattgc ccgagctgta caagagcgta accggcccgc gcaggatttc
     2101 gacctgttgc gacaaggcgg tatctaccat aatggcgtga tcgggcgaaa aatccgccat
     2161 atcgcctgtt tcgccgtgat ggttcaacac tttaatccgc ctgcctgttt gaccgcgaat
     2221 aacgggagcg gacgcgccgc cgccgtattg cgaggcgtga atgcccggca cgccgtccaa
     2281 agcatcgccc aagttgacgg ctttttgtcg caaggtgtcg ccgctgatga ttttgtcgga
     2341 ggcggtcgaa gtgtgcagca gcccggatgt ggcgcgcgga cggcttttgc cgacgacggt
     2401 aaccgtttcc aagcccaccg attgctcagt ttcatgcgct tgggcgagga ggggtgtgtt
     2461 gattaaaaga attgataaaa caatgggttt gagtgtagtt tgtgccattt tggcttctcg
     2521 tcgcatttca aaagtttgtt attatataac attacatttt ttatatcata agattttgag
     2581 aacactcaga gggtataggc aaaagttttt caaatgaaac ggttgcggca tcgggcggtg
     2641 tccatttgta tccgccgtca ttcgggggcg cggttgatgt tgacgcagat tccgctgtgt
     2701 ttgcccatta tgttcggctg cgggataacc aaaatttatg agtgcataaa aacggtacgt
     2761 tcccgaacgg tcgaaaaggt ggcaaaatgg cgtacttgtc agaacgcgag gctctgcgcc
     2821 aggttcacga gggtgcatcg ggcacgctaa gatataagag ggtatggatg ggggtatcgg
     2881 aaatgcagtt aataacaaac aaattataaa tcaataggtt aataacaaaa tgcttttgtt
     2941 tatcgacaat tacgacagtt ttacttacaa catcgtccag tatttcgctg aattggggca
     3001 ggaagtcgcc gtgcgccgca acgatgatat tacgttggaa gaaatcgagg cattgaatcc
     3061 gcaatatctc gttatcggac ccggtccgtg ttcgcccaaa gaagcgggga tttccgtggc
     3121 ggcgatgcgc cattttgccg gccggctgcc gattatgggc gtgtgcctcg ggcatcagac
     3181 gataggcgag gcgttcggcg gcaggatagt ccgcgccaaa acgctgatgc acggtaaggt
     3241 gtcgcccgtg tcccattcgg gcaagggtat gtttaaaggt ttgcccaatc cggttacctg
     3301 tacgcgttat cacagcctcg ttatcgatcg ggataatttg cccgattgtt tggaaatcac
     3361 ggcttggact gacgacggcg agattatggg cgtgcgccat aaggaatatg ccgtcgaggg
     3421 cgtgcagttc caccccgaag ccctcttgac cgaacacgga catgatatgt tgaacaattt
     3481 tttaatcgaa tttcaaaact tcaaaccgca aaaaatctga cgcgatgccg tctgaaggct
     3541 tcagacggca ttttcgtccg aatattgaac ggaggacaaa aaatgattac accgcaacag
     3601 gctatcgaac gattaatcag caataacgag ttgttttacg atgaaatgac cgacttgatg
     3661 cgtcagatta tgagaggaca ggttctgccg gagcagatag cggccatttt gacaggattg
     3721 cgtatcaagg ttgaaaccgt ttccgaaatt accgcagctg cagccgtcat gcgcgagttt
     3781 gcgacaaaag tgccgctgga gaatgcagag gggctggtcg atatcgtcgg tacgggcggg
     3841 gatggtgcca aaaccttcaa tatttcgacg acctcgatgt ttgttgccgc agcggcaggc
     3901 gcgaaggttg ccaaacacgg cgggcgttcc gtttcatctt ccagcggcgc ggcggacgtg
     3961 atggagcaga tgggcgcaaa cctcaacctg actcccgaac aggttgccca aagtatcagg
     4021 cagaccggca tcgggtttat gttcgcgccc aatcaccaca gtgccatgcg ccatgtcgcc
     4081 cctgtacgcc gttcgctcgg tttccgaagt attttcaaca tattgggtcc gttaacgaat
     4141 cctgcgggcg cgccgaacca gcttttgggc gtgttccaca ccgatttgtg cggcattttg
     4201 tcgcgggttt tgcaacaact tggttccaaa cacgttttgg tcgtttgcgg ggagggcggt
     4261 ttggatgaaa ttacactgac gggcaaaacg cgcgttgccg aactcaaaga cggaaaaatc
     4321 agcgaatacg acatccgccc agaagatttc ggcatcgaaa cccgccgcaa tttggatgaa
     4381 atcaaagttg ccaatactca ggaatctttg ttgaaaatga atgaggtgct ggaaggaaga
     4441 gaaggggcgg cacgcgatat cgtattgctc aacaccgccg ccgccctgta tgccggaaat
     4501 gtcgctgctt cgctttcaga cggcatatct gccgcacggg aagccatcga ttcaggcagg
     4561 gccaaatcga aaaaagagga gtttgtcggt tttcaaccac aacaaagatg ccattttctt
     4621 ggaaagatgg agcttgggtg atgccgccat gattatggaa cttttgtggc aaaacataag
     4681 cacttcacga agagaactta ccaaactgtt tttatataaa aacttggggc tgtactagat
     4741 aaccagacca aattcccatt aactaattgt cttaaaatct gaatttgaga ttctatttaa
     4801 aatgccattg gcatttcttt aaatgcagcc ccaaatgctc tttgggaatg ccgttaaact
     4861 tacgtaaatg gctaaattca ctaatatcaa gcacatcata actacgaaag gtatccgtgt
     4921 atacaatgcc atcaggctta actttctttc ggataattgg caacaatgtc gctgattgcg
     4981 cattaggaac aacgacggta taaaccttat atattgcgtc cctaagaagg gacgattaac
     5041 aaaaattaac gtcctttact ttctacaagt aacagggctt ttttttgccc gtttttgagg
     5101 attcgcacca tggaagataa gcaagggatg acaaaggcgg ttgccggcgt gatgacggac
     5161 gcgccgacgg acggcaggaa gccggcaacc gcttcaaa
//
LOCUS       DAWKQJ010000132         5179 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000132 DAWKQJ010000000
VERSION     DAWKQJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.132"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="WJL20_001630"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="WJL20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ7683763.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="WJL20_001631"
     CDS             complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="WJL20_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ7683764.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="WJL20_001632"
     CDS             complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="WJL20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ7683765.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2313..3839)
                     /gene="farB"
                     /locus_tag="WJL20_001633"
     CDS             complement(2313..3839)
                     /gene="farB"
                     /locus_tag="WJL20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ7683766.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3863..5017)
                     /gene="farA"
                     /locus_tag="WJL20_001634"
     CDS             complement(3863..5017)
                     /gene="farA"
                     /locus_tag="WJL20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ7683767.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1338 a         1456 c         1227 g         1158 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggtccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtctgg gtatgtggga caaaattccc gaaaatgaaa tttacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgtttc tcacaaaccc gccgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac aactcaaggg
      961 taggcttggc gacatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttaca gcacacggtt tcaacttggg gctttccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac ggaaccaata tgatgctgaa
     1261 actgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc acttggtgct
     1501 tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag attcgcatac
     1561 ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc gaacagtcgg
     1621 aggctttgct ttcaaactaa aattatttca gtttggtttc tttgtacact acgtgtttgc
     1681 gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt ttgtttttgg
     1741 tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta tcgcgcattg
     1801 cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg caaatcagcc
     1861 aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga aacgcgcagg
     1921 cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt gggcaaaaaa
     1981 cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg gcgcttaccg
     2041 gtcactttgc aaactcgtgc catggtcagt ctccaaactt ctaaaatagt aaaacgcgat
     2101 ttataccacg aaaaacagtg ttcgttcaag tgttttagag aaaaacggct gttttacgcg
     2161 aattatcctg ccgattgttg cattcgggaa acgtttgccg atacgaaaca gacggaatgc
     2221 gattatagcg ggtttcacag atgcctgcat tctaaatact gtccgaacat atttccagta
     2281 ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc gccgttgtgg
     2341 aacggcggtt ttgccagcca tatgacgggt atcatgataa tgaataacaa gctgcccgcc
     2401 ataaagattt cgttcgagcc gataataaaa ccctgctggg taatggtatt gttgatgatg
     2461 cctagggttt gaatgtcgga aacgccgtgc tgggacaaat gagcggccgt ttcgtgcaat
     2521 gttgcggaat agggcgtgat gtgttcggca aagcgtgtat ggtgcaacgc ttcacgccgt
     2581 tcccacaggg tactgacgaa tgatacgccg acaccgccca tcagcacgcg caagaaattc
     2641 gacaggctgc cggcggcggc aatctgcccg cccttcatat gcgacagtgt gatggtggtc
     2701 agcggcagga agaacatggc aacgcctgcc ccctgccaaa actgcggcca gatgacgttg
     2761 ccgatatcca tatcggcata aaaatccgta cgccaataga aagtaaaggc aaaggtcagg
     2821 aagctggcag ttacgaacag gcgcatatcg attttattgc cgaacctgcc gattaacgga
     2881 gacaggaaaa caggcaggat gccgacaggt gcggcggcaa gccctgccca cgtggaggta
     2941 tagcccaggt tggtctgcaa cactaacggc agcagggtca gcgtccccat atacaccata
     3001 aaacccaatg acgtggcaat gacgccgacg gtaaaattcc gatctttaaa cagcgataaa
     3061 tcgacaatcg gatatttttc tcccaattcc caaacaataa aatacgacaa gcacaccagt
     3121 gcgactacgc ccaaggtaat gatttctcca gaggcgaacc agtcgagttc cttacccctg
     3181 tccaacatca tctgtaacgc gccgataccg actaccatca atgtaagccc gacatagtcg
     3241 gtcggcattt taacggtttc cgtttcccga catttcaaat gtttccatgt aatccatgcc
     3301 gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca gtttccggaa
     3361 atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat tgcccacaat
     3421 gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct ttgcgacaag
     3481 ggaatcagcg gcccggcgat aaagccctgc aagatgcgga aaacaaccag cgactgaagg
     3541 ttgggggcaa taccgcacag ccacgatgtg atgacgaaac cgacagcggc ggcggtaaac
     3601 aatttgacct cgccgatacg ttttgccaaa aagcccgtta acggcacgga aacggcgttt
     3661 gccacagaaa aggaagtgat gacccacgtc ccctgcgtgg ttgccgcacc gaggttgccg
     3721 gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat aaatacggca
     3781 agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg ataatccatt
     3841 gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca tcgaccgtcc
     3901 gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc ggcgtttttg
     3961 aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa ccgatacgca
     4021 acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg cgctgcacca
     4081 ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg ctgcccgtac
     4141 ctgccgaaaa acctgccacc ctgccgcgat aaacaatttg tttgccgtac aaatcggaca
     4201 ccagctcggc aggctgtccg attttcatat gccgcaactg cgtctcttta aaattagcat
     4261 ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc gccacctgct
     4321 gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg atttgcgtcc
     4381 gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc gccggctgtt
     4441 cgcgcaaaga aacctgaccg cccaaagccg cacgcgccga agattcttcc gccaaagccg
     4501 ccttaactgc cgcctgagcc tgagacactg ccgcacgggc atgtgccagc tcttcggcgg
     4561 acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgc gcgcgcgcca
     4621 aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca ttttgccggc
     4681 ggttttgccg caccgcctga atcagctcgt tttttgcccg ctcgtaagcc agcacatcat
     4741 tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta tcgtcgtgca
     4801 aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt ccggcaacat
     4861 aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaacgccgac ccggcggctg
     4921 cggcggcaag cgcgaacagc agcgtcaatg ccgtcaggcg gcgtttgcgt ttggcttgcg
     4981 tgttttgaag ttttgtttcg tccgcgtgcg tatccataag attgggttcc gaatttcctg
     5041 atttcatata aacaatgttc tttcaatagg aagcagattc tatatccgcg caataacgca
     5101 atcaagtcag tattttagat gcaatattca aaaaacaacg cttcaggcgg caaaactgaa
     5161 aatatatttc gtttatata
//
LOCUS       DAWKQJ010000133         5101 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000133 DAWKQJ010000000
VERSION     DAWKQJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.133"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..768)
                     /locus_tag="WJL20_001635"
     CDS             complement(85..768)
                     /locus_tag="WJL20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ7683768.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVALLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(925..1191)
                     /locus_tag="WJL20_001636"
     CDS             complement(925..1191)
                     /locus_tag="WJL20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ7683769.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(1264..1734)
                     /gene="rlmH"
                     /locus_tag="WJL20_001637"
     CDS             complement(1264..1734)
                     /gene="rlmH"
                     /locus_tag="WJL20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ7683770.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            complement(1788..2174)
                     /gene="rsfS"
                     /locus_tag="WJL20_001638"
     CDS             complement(1788..2174)
                     /gene="rsfS"
                     /locus_tag="WJL20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ7683771.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(2233..2826)
                     /gene="nadD"
                     /locus_tag="WJL20_001639"
     CDS             complement(2233..2826)
                     /gene="nadD"
                     /locus_tag="WJL20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ7683772.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSIRIL
                     SAPMHNASSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            complement(2823..3422)
                     /locus_tag="WJL20_001640"
     CDS             complement(2823..3422)
                     /locus_tag="WJL20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ7683773.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVELIRRHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(3451..4992)
                     /locus_tag="WJL20_001641"
     CDS             complement(3451..4992)
                     /locus_tag="WJL20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ7683774.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGGQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAGSDKAVAPVMPSPPQSVEEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
BASE COUNT         1184 a         1553 c         1361 g         1003 t
ORIGIN      
        1 tgcgtgcatc cgtattgtat gcccaaagta aaatgccgtc tgaagcattt tcagacggca
       61 tagtcggacg gcgttttacc ggcatcaatc ctcgccgttt aaagacaaca ggatgttgag
      121 caggctgctg aagatgttgt aaagcgagat aaacagtgtc agtgccgcgc tgatgtggct
      181 gtcttcgccg ccgtcgatga cggtgcgtac ctgccacata atcattaagg aactgaacaa
      241 gacaaaaccg gcggaaatgg tcagggcgag tgcgggaata cccaaaaaca gattggcaac
      301 cacggcgacc atcagaatga ccgcacctac ggtcaggaag cgtccgagcg cgttcatatc
      361 gagccgggtt cggcgcgcca aagcggacat cgttaaaaag acggcggcgg tcatcgcggc
      421 ggcaatgccg acgattttcg caccgtcggc aatatggagc gcgtattgca gcacggggcc
      481 gatcaatacg cccataccga atgtgaatac catcagcagg gcaacgccgg tattgctgta
      541 acggtttttc tcgatgaagt ggatcatacc gtagaaaaac gccaacacga cggcaaaccc
      601 tatccagcgc gaaccgaagg cggcgtaaaa attgaaaccg gcattggcgg caagtgccgc
      661 gcctgcggca gccggaatga atgaaaatcc gagcaggcgg taggttttct gcaggacggt
      721 gtttttagaa accgtatgcg cggtgtagtc gtaaacgtcg tgttgcatat catctgctcc
      781 tgaaagcgcg gttgggaata atgggggatt ttaacattgc ccaatgtcaa aatttgtccg
      841 gttgcgtgaa gataaagttg tccggcgtat tttaaaggcc gtctgaagca gtttcggaca
      901 gcctgtgttc aaaacggaaa accgttattg cggaacgtat ccctgcacgg catccgcgcc
      961 gtcgccgaag aaatactgct ccatctgctg agccaggtat tcgcgcgcgc gcggatcggc
     1021 gaggcttaaa cggttttcgt taatcagcat cgtttggtgg cgcgtccacg ccgcccacgc
     1081 ttcttgcgat acgttttcaa aaatgcgctt gcccaattcg ttgggaagcg gcggaaattt
     1141 catgccttcg gcttctttgt tgagcttgac gcagaatacc atgcgtgcca tagcggattc
     1201 ctttgctgtg ttcagaaata acggggtaat tttaaccgat tagggatacg gacaaaagcc
     1261 ttcttattcc cgatgatagg gatggttgtg caggatggag gcggcgcggt agagctgctc
     1321 ggtcagaagg acgcgcacca tcccgtgcgg cagggtcagg ctggacaggc gcatcatcat
     1381 gcgtgcctgc tgtttgaggc ggtcggtcat accgtccgcg ccgccgatga cgaagcagac
     1441 gtgttcgccg ttttgctgcc agcttttgag gtgttccgcc agctcgacgg aggtcggtgc
     1501 tttgccgcgt tcgtcaagaa cgacgaggaa cgcgccttgc ggaatggctt caaggatgcg
     1561 tttttcttcc gccgccatac cttgggcggc attcacgccc gcgccgcgtt tttcgggttt
     1621 gatttctttg agtgcgtagg cgacgtcgcg tccgaagcgt ttggcgtatt cggcgacggc
     1681 ctcatcaacc cagcgcggca ttttggtgcc gactgccaaa acggtgatgt tcaatgcttt
     1741 ctcccttaca ggaaaatgcc gtctgaacat tcagacggca tcgggaatca gtctgccgcg
     1801 tgccacggct tctgcattcc ggcgtggaaa ctcggtttct cgccgcccca aagggtgtcg
     1861 atatcataga agtcgcgcac ggcggggagc ataacgtgga cgacgaggtc tcctgcatca
     1921 accaacgtcc attcgccgct gtcgccttcg gtactgagga tttcaaaacc ggcttctttc
     1981 aaatcgacgg caacgttgtt ggccagtgct ttgacttggc gcgtactgtc gccgctggcg
     2041 ataatcattc tggcaaacag cgaagttttg tcttgcgttt cgagaacgga aatgtctttg
     2101 gctttgatgt cttcgagggc gttgacggcg accccgacca ttttttgcag gtcttgcagt
     2161 tcttgttcgt tcattatttt cctaacggga tgttttcaga cggcattata gccgtttctt
     2221 actgatttga ctttattttt catacaaacc gtgttcgcgg atgtagcgtg cggcggcagg
     2281 tgggatgccg tctgaaacac cggcgcggcg gatttccgtt gacgacgcgt tatgcatcgg
     2341 ggcggacaag atgcggatgc tgccgtcctg aagggcattg cccagccatg cgtgcagttc
     2401 gcgcggggtt tggtgcaggc tgtcgccctg ccgcatggcg acggcgatat tggtttcgcg
     2461 cacgagcatc tgccattttt tccaagtatg cagcttcatc aggctgtcgc tgcccatcag
     2521 ccaccagagt tgcgcggacg ggaactgctg gcggaagatt tggacggtgt cgaaagtata
     2581 ggttgcacct tctcggacga tgtcgcagtc gctgacggca aaacgcgcgt cttctgccgt
     2641 cgccagttcg accatggcaa ggcggtcggc ggcggaagcg gaggcggcgt ctttgtgata
     2701 cgggccgcct gtcggcagga aaacaaccgc gtccaagccg atttcgtcgg caaaggcgcg
     2761 ggcgatgtgg agatgcccgt tgtgtatcgg gtcgaaagtg ccgccgaaca gtccgatttt
     2821 cttcatgatg tttccattcc ttccgataag tccatgccgt ctgaaacact gccgtccaca
     2881 accagcgtca gtttgccggt aacgggatgg atgaccaatc cgtgaacggc gatatggcgc
     2941 ggcatcagcg gatggcggcg gatgagttcg acagtatggc gcacgctgtc ttcgacgttg
     3001 tcgaaaccgg tcagccagcc gtcgaggtcg acacctgcat aacgcagggt ttcgatacgg
     3061 tcttcgggaa tccggctttc ccggacgcgc ccgaggaatt cttcggcatt cagcccctgc
     3121 ataccgcaat cgtgatgggc gatgaccata atctctctga ccttcaattc aaacacggca
     3181 accaaaaggc tccgcatcac cgaaccccac gggtgcgtaa ccagcgcgcc ggcattttta
     3241 atcagcttgg catcgccgtt tttcaaaccc aacgcgtcgg gcagcagccc gataatccgc
     3301 gcatccatac aggacaaaac tgccagcccg cgttcggggt atttgtcggt aaagtatttt
     3361 tcatattcgc ccgactcgac aaactgccgg ttatgggcaa ggatgttatc caactcgctc
     3421 attttgccgt cctctgaaaa agggttcaca ttataacgtt tccgtctgtt ttccgccttc
     3481 gccgttgtcc aacagcagga aaatgcccag cgcgaacgcc gacaacagca atgtcaacac
     3541 cattgcccgc gcataattat cctcacccgc acgccccaaa taggcataaa tcaaagtcgt
     3601 cagcgtctgc cactcgggac gcgacaggaa caaggttgcc gcaaattcgc ccacacacgt
     3661 cgccgccgcc aaagtcagac cgcgccgcaa cgccggtttc aagaggggga acgtgatgcg
     3721 gcatgccgtc tgaaagccgt ttgcacccaa acccgccgcc gccctgccgt aatccggcgg
     3781 cagtgcatcc caggctgata aaacatcttt tgccacaaac ggatacgcca gcagcgcata
     3841 catcgccagc agcagcggca acgaagccgt ccactgcgga taaagcagca gcacgcccgc
     3901 cgaaacacaa accggcgaca ccataaacgg caaaaacatc agcccgcgca tccacgccga
     3961 ccgccgcgcc gccgccgcat acaccacacc caaaaccgcc gccgcataca ccgccgctgc
     4021 cgagaagcgc aaagtattcc acaccgcctg ccacgtttca ctttccatta acacacgcca
     4081 cgattcgccg gccgaccacg ctttcacaac aattgccaac aaaggaaaca ggcagcacac
     4141 agacaacacc gccgccgcaa acgccagcag cacatattcc tccaccgatt gcggcggcga
     4201 cggcatcaca ggggcaaccg ccttatccga acccgcgcgt ctgccgaacc acgcatacag
     4261 caatcccgcc gccgccgtta cccccaacac cagccacacc agcaccgagg caaccgccat
     4321 atcgagttcg aacatgacca actggtaaat ttccacttcg accgtggcat aacggctgcc
     4381 gcccagcagc agcgccagcc cgaacccgga aaaacaatac agaaagacaa ggcacacgcc
     4441 gccagcaagc cacgggcgca aaacgggcat ttcaatgtcc caaaaccgcc gccacgcccc
     4501 cgcacccaac gtccgtgccg tctgaagccg tgccgcaggc acttgcacaa acccctgata
     4561 cgccgccctg accaacacag gaaggttaaa aaacacattg ccgtacaaca acagatacgg
     4621 cgtatcctgc ccgccgcgcc acaacaggcc gtccgccccg aacagagcca gcacgcccac
     4681 gcccgccacc aacgtgggca tcacaaaagg cagcatcagc aggcgcagca ccaaagcccg
     4741 ccccggaaac gccagccgcg ccagcaccca cgcgacaggc acgcccaaag gcagcaccag
     4801 cacacaggtt gccgctgcct gaaataccgt ccacgccaaa cgtttgagca tataggcatc
     4861 cgacagcacc gcgcgccacg ccaaaccgtc atacgccgcc accgcccaca aaggcgcaac
     4921 gaccattacc gccaaaaaag ccgaaggcag cagggcaaaa gcaccccata ccgcccaacg
     4981 ccgtccatcc atcgccttcc ccacttgaaa cactgatgtt gcgattgtac ccaaaagccc
     5041 ccacataccg tatatttcaa tccgactaca taccgtatcc gccttcctcc cgccgtctga
     5101 a
//
LOCUS       DAWKQJ010000134         5019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000134 DAWKQJ010000000
VERSION     DAWKQJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.134"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(61..994)
                     /locus_tag="WJL20_001642"
                     /pseudo
     CDS             complement(61..994)
                     /locus_tag="WJL20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            complement(1006..1407)
                     /locus_tag="WJL20_001643"
     CDS             complement(1006..1407)
                     /locus_tag="WJL20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7683775.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAHDRLLNRKQQRRDSPANTFKHR
                     LIRALTKISREREKRRQRREQLIGKIIVPFQ"
     gene            complement(1424..3487)
                     /locus_tag="WJL20_001644"
     CDS             complement(1424..3487)
                     /locus_tag="WJL20_001644"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ7683776.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMADDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(3508..3918)
                     /locus_tag="WJL20_001645"
     CDS             complement(3508..3918)
                     /locus_tag="WJL20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ7683777.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(3995..4900)
                     /gene="glyQ"
                     /locus_tag="WJL20_001646"
     CDS             complement(3995..4900)
                     /gene="glyQ"
                     /locus_tag="WJL20_001646"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7683778.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEEASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
BASE COUNT         1024 a         1318 c         1405 g         1272 t
ORIGIN      
        1 ctgcaattta ttctcccgat aggcacgatg ccgtctgaag gcttcagacg gcctgccccg
       61 tcaataaatc ttgcgtaaga atctggcaga cagctttctg cgccatcttt gaagcatacg
      121 ctttgtcgga aacacacggt gcgggacggc aagtttgccg cgccattctt cgggaacgct
      181 cgtcaggctg cccgccaatt ctgtgaaacg ttccgcaccc cacgcggtgc agtccctgtg
      241 ccacggcttt gccgggccgc aataatggct gacggcgaca ggcatcgccg tataagtccg
      301 gtcgcggtaa agcgggtcgg tatggcggga cgcaaacctg ttcgccataa aggcatcatt
      361 ggtcggcata aagttgaaac ggctgttcgc ataacacacc ccgcctttaa acagcccgtt
      421 caaaatatcc tgatcctgat attgcatcac gtccttgtat tgttccaccc attcgcaggc
      481 cattttgaaa atatcgtgct gccgccactt tttcaggttg atcagcaata cgccggcatt
      541 gaaataatat tctccgtccg ccataccgat tttttgtttg taagcattct gcctttcgac
      601 aaacaaatcg atgcacgcgc caagccagtt atcgcccaaa tcggtatccc ataagggctt
      661 caggctgtcc ctgaccaata cgtctatatc cagatacagg actttatcgc aatcggcaat
      721 gtattcgccc aatttcaagc gggcataagt cgtgatggaa atgtgcctga tgtttaaggg
      781 gaagccggcg aaatcttcgg ggtttacgtc tataaagcgg atattacccc cccccccgca
      841 aattggcagc aaccgccgcc cggtttgcct cactgatgcc ggcatcgagg acgtggaacc
      901 tgatttccgt atcgggatgg gccgcttcca cgctttttgc cgcaacgcaa agataggcgg
      961 catagttgtc gtctgccgca aatacgatgt ccatcttttc tccttttatt ggaaaggcac
     1021 aatgatcttg ccgattaact gttcgcgcct ttgccgacgt ttttcccttt ccctgctgat
     1081 tttggtcaag gcgcggatca ggcggtgttt gaatgtgttg gcgggggaat cgcgcctttg
     1141 ctgtttgcgg ttcaggaggc ggtcgtgtgc aaggattttg atacggctgt cccgcttttg
     1201 aaaatccttg gcaatggcaa gcgtaccgtc tgtcgagccg tcatcgacaa tcaaaatctc
     1261 caagttgcgc caagtctgat tcacgacggc ggctaatgat tgggcgaaat atttttctac
     1321 gttgtaggcg caaatcaata cgctgactaa aggctgcaat ttattctccc gataggcacg
     1381 atgccgtctg aaggcttcag acggcatttg gactgtacaa cggttactcg cccaaaagcg
     1441 caatgtcggc taccgcgttc atctgctctg ccaaacggtt cagcagattc aaacggtttt
     1501 gcttcacggc ggcatcgtcc gccatcacca tcacgccgtc aaagaaggca tcgacttgcg
     1561 gcttgacgga agccagttcg gacaaggcgg tttggaaatt gccttcggca acggcggcgg
     1621 cgattttcgg ctgcaaacct tgcgcggcgg caaagagggc tttttcttcg tcctgttgca
     1681 gcaggctttc gttaaccgcg cccaactcgg catcggcttt tttcagcaga ttttgcacgc
     1741 gtttgttggc ggcggcgagc gcggcggctt cgggcagttg tttgaacgcg gcaacggctt
     1801 gcagtttcgc cactacgtcg tccaaacggc gcggctgttt cgccaatacg gcggcaacga
     1861 tgtcttgcgg ataatcgttt tgcagcaaca cggcaaggcg cgcctgcata aagtcggcgg
     1921 tttcagacgg cgtttgctcg ttgagcaagc ctttggggaa gctgtcgaat gccgtctgaa
     1981 tcagttcgtt cacgtccaaa ccgtactgca tcagcatccg caaaataccc aaggcggcgc
     2041 ggcgcagggc gtaggggtct ttgtcgccgg tcggaatcag accgatgccc caaatgccga
     2101 ccagggtttc cagcttgtcg gcaagtgcaa cggcagtggc aattttgctt tcgggcagct
     2161 tgtcgccggc aaaacgcggt tggtaatgtt gctcgacggc ttcggcgatt tcttcggttt
     2221 cgccgtccaa gcgggcatag tatttgccca tcgtgccttg cagttcggga aactcgccga
     2281 ccatttcggt taccaagtcg gctttggcaa gacgcgcggc gcgttcggca gcggcggcat
     2341 ccgcgcccaa tgctttggcg atgtgggcgg caatgctttg caggcgttcg atgcgttcgg
     2401 cttgcgagcc gattttgttg tggtacacca cgctggacaa tttcggcagg cggctttcca
     2461 aagtcgcttt ttggtcttgt ttgtagaaga actcggcatc agacaggcgc gcgcgcaaaa
     2521 cgcgttcgtt gcctcggatg atgtgcgacg ggtcttcggt ttgcaggttg gaaaccagca
     2581 ggaagcggtt catcagcttg ccgttttggt cgagcagcgg aaagtatttt tggttttgct
     2641 gcatcgttaa aatcaagcat tcctgcggca cggcgaggaa atgttcctca aaaccggctt
     2701 ccaaaacaac aggccattcg accagcgcgg ttacttcgtc caacaatgct tcatcggcgg
     2761 cgacggcggc gttcagactg cgcgcctgcc cttccaatgc cgtctgaatc gcggctttgc
     2821 gctcggcaaa cgaagcgacg actttgcctt gcccgcgcat ttgttcggcg tagctgtcgg
     2881 cgttttcaat ggtgatttcg ccgttggaca agaagcggtg tcccaaggtt tgattgctgc
     2941 tttgcagacc caaaacgctg acgtttacga tgtcgccgcc gtgcagcaca atcagcccgt
     3001 gaacagggcg cacgaaggta aatgtgctgc tgccccaacg catcactttg ggaatcggca
     3061 gtttcttaac cacttgattg ataatgtctt ccaaaagtcc gcccagcggc ttgccggttt
     3121 ggacgtattc gtaggcgtac acatcctgct tgccgtcgtg gacgatggtc aagtcttcga
     3181 ttttcgcgcc cgcaccgcgt gcgaaacctt ccaaagcctt ggttggcgca ccgtctttca
     3241 tggcattcgc cacggcaggg ccttttttca cgattttttg gtcggcctga acggctttga
     3301 cgtttttgac ttgaaccgcc aaacggcgcg gcgaggcata agccgtaaat tcggctgcac
     3361 cgtcaaccag ttgcgctttt tccaagcctt cggcaacggc ggcggcaaaa tggttgccca
     3421 gattattcag ggctttgggc gggagttctt cggtaaggag ttcgattaaa agggtttggg
     3481 tcatcattcg gctttctttg aatttggtta atctgcctgt ttataggttt cgctgtaatt
     3541 ttcccagccg tcatccccgt aaaaaccatc aaccagcggg gtggcgtaca aagtggcgac
     3601 atcttcgcga cctgccagcc aagagataat ggcttttttc tcggtttcgc ccaagcttcg
     3661 ggcgccggat ttttgaaaca ggcacgaaaa atcgccgcaa tcgcccccac ccgccatttc
     3721 caagccgttt gccgcaagat acgcaatcag ctcgtccata aagcggccga acgcttcggc
     3781 atcgtccgca gcttggtgca aactgccttg aacgccgaaa atcaatgttt gaaactcgcc
     3841 caaatgcagc tttttatgct ggcgacggtt cattttgtgc aggcgtttcc tgcttggggt
     3901 gcggaaatag acaggcatga ttttcctaaa atatataatg gtttacggac ggctgcctta
     3961 tcgtgccgcc cgaacgtaaa aaatcgtcgc ccccttaggc ggcatttgcc ttcattaagg
     4021 gaaagcccag tttttcgcgg ctttcgacat atttttgcgc cacggcgcgg ctcaacgcgc
     4081 ggatgcgccc gatgtaggtg gcgcgttcgg ttacggaaat cgcgccgcgt gcgtctaaaa
     4141 ggttgaacgt atgccctgct ttgagaacca gctcgtaggc gggcagcgca aggcttgctt
     4201 cttcaacggc gagcaggcgt ttggcttgcg cttcgtagtc gttgaactgg cgcagcagcc
     4261 aatcggcatc gctgtattcg aagttgtagg tggattgttc gacttcgttt tggtggtaca
     4321 cgtcgccgta agttactgta ttaccgtcca gcgtttttgc ccaaacgagg tcatagacgt
     4381 tttccacgcc ttgcaggtac atcgccaagc gttcgatgcc gtaggtgatt tcgccgagta
     4441 cgggggtgca gtcgatgccg ccgacttgtt ggaaataggt gaactgggtt acttccatac
     4501 cgttgagcca gacttcccag cccaaacccc acgcaccgag ggtggggttt tcccagtcgt
     4561 cttcgacaaa gcggatgtcg tgcactttgg ggtcgatgcc caattcgcgc agggagtcga
     4621 gataaaggtc ttggatattg gcgggggcgg gtttgagggc gacttggaat tggtaatagt
     4681 gttgcaggcg gttggggttg tcgccgtagc ggccgtcttt ggggcggcgg ctgggttgga
     4741 cgtaggcggc aaaccaaggc tcgggcccga gcgcgcgcag gcaggtggcg ggatgggaag
     4801 tacctgcacc gacttccatg tcgaagggtt ggatgacggt gcagcctttg tctgcccaga
     4861 atgtttgcag tttgaagatg atttgttgga aggtaagcat ggcttatgat tcgataaaat
     4921 aaagggttta ttttactgtt tccattgctg tttggatagg tttatctcga agacagactg
     4981 atttgaaaac acggcataca tgatatagtg gattaaatt
//
LOCUS       DAWKQJ010000135         4955 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000135 DAWKQJ010000000
VERSION     DAWKQJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.135"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            244..4908
                     /gene="iga"
                     /locus_tag="WJL20_001647"
     CDS             244..4908
                     /gene="iga"
                     /locus_tag="WJL20_001647"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ7683779.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITDPKTISIHYIQNNDDDDDGYYYYRPRKP
                     IPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFDATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAEKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAA
                     EIAEQKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREA
                     AELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEI
                     PLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQ
                     ADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDY
                     ASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYG
                     KYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRASIQTGLTLGNTLKINQFEIVPSAG
                     IRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYG
                     KGGVNVGGNSFAYKADNQQKYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQI
                     RF"
BASE COUNT         1684 a         1154 c         1063 g         1054 t
ORIGIN      
        1 cacaagcggg aatccaaaaa caaaaagccg cagaaattta acagaagaac cgaaccccga
       61 aaatcggctt cccaccttcg cctgaataac agaacaaagg aagcggaaat aaaaacatcg
      121 ggattctttt tttaaattgg caataaaaca ccaaaatgaa taattcacac aacaaaaaat
      181 agtcaacacc gccaaaccgg cgtaaaatac aaaacattat cacccttaaa acggtaaaac
      241 cttatgaaaa ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
      301 gcccttacac catactcaga agcggcattg gtcagagacg atgtcgatta tcaaatattc
      361 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
      421 aacaaacaag gtcaaaacat cggcaacgca ttatccaacg taccgatgat tgattttagc
      481 gttgcagatg tcaacagacg tacattaacc gtcatcgatc cccagtatgc cgtcagcgtc
      541 aaacatgtaa aaggagacga aatctcttat tacgggcatc acaatgggca cttagatgtt
      601 agcaatgatg aaaacgaata tcgttcagtt gcacaaaatg actacgaacc aaataaaaat
      661 tggcatcacg gtaatcaagg tcgtcttgaa gactacaaca tggcacgcct taataaattc
      721 gtaacagaag tcgcacctat tgcaccaacc agtgcaggcg gcggcgttga aacctataaa
      781 gataaaaacc gtttttctga gtttgtgcga gttggagcag gtacgcaatt tgaatataac
      841 agccgctaca atatgacaga gctttcacga gcttatcgtt atgccattgc aggtacgcct
      901 tatcaagacg tgaatgtcac atcgaatcta aatcaagaag gcttgattgg cttcggtgat
      961 aattcaaaac atcattcccc agaaaaactc aaagaagttt tatctcaaaa cgcattgact
     1021 aactatgctg tgttaggcga tagcggttca ccattatttg cttatgataa acaagaaaaa
     1081 cgctgggtct ttttaggtgc ttacgattat tgggcaggct atcaaaaaaa ctcttggcaa
     1141 gaatggaata tctataaaaa agaatttgca gataaaatca aacaacgcga taacgccggc
     1201 accatcaaag gttatggcga acatcattgg aaaaccacgg gcacaaacag ccatatcggt
     1261 tcgacagcgg taaggcttgc caacaatgaa agggatgcaa acaacggaca gaatgttacc
     1321 tttgaagaca acggcacttt ggtattggat caaaacatca accaaggcgc gggcggtctg
     1381 tttttcaaag gcgattacac agtcaaaggt gcaaataatg acatcacttg gctaggtgcg
     1441 gggattgatg ttgccgacgg caaaaaagtc gtttggcaag tcaaaaatcc gaatggcgac
     1501 agattggcaa aaatcggcaa aggcacatta gaaataaacg gcacaggcgt taaccaaggg
     1561 caattaaaag tcggcgacgg tacggttatt ctgaatcaac aagccgatgc cgacaaaaaa
     1621 gtccaggctt tctcccaagt cggcattgtg agcggacgcg gtacattggt attaaatagt
     1681 tcaaatcaga ttaatcccga taacttatat ttcggtttcc gtggcggtcg tttggatgcc
     1741 aatggcaatg acttgacttt tgaacacatc cgcaacgtgg atgaaggcgc gcgcattgtc
     1801 aaccacaaca caagccacgc ctccacaatc acgctaacgg gtaaatcttt gattaccgat
     1861 cccaaaacca tctctattca ttatattcaa aataatgatg atgacgatga tggttattac
     1921 tattaccgcc ctaggaaacc gattccacaa ggcaaagatc tttatttcaa aaactaccgt
     1981 tattacgccc taaaatccgg cggcagcgtg aacgcaccga tgcccgaaaa cggacaaacg
     2041 gaaaataacg actggatttt aatgggcagc acacaagaag aagccaagaa aaacgcgatg
     2101 aaccacaaaa acaatcagcg tattagcggt tttagcggtt tctttggcga agaaaacgga
     2161 aaaggacata acggtgcatt aaaccttaat ttcaacggca aaagcgcgca aaaccgtttc
     2221 ttgttaacag gcggcactaa tttaaacgga aaaataagcg taactcaagg caatgtcttg
     2281 ttatcaggtc gtccaacacc acacgcaaga gattttgtga acaaatcttc agcccgaaaa
     2341 gacgcacatt tctccaaaaa caatgaagtc gtatttgaag acgactggat aaaccgcaca
     2401 ttcaaagcca cagaaattgc ggttaatcaa tccgcatcat tctcttccgg tagaaatgta
     2461 tccaacatca ccgccaacat caccgcaaca gacaacgcca aagtaaattt gggttacaaa
     2521 aacggcgatg aagtttgcgt gcgctcggac tataccggtt acgttacctg caacacagac
     2581 aacttatccg ataaggcttt aaacagcttt gatgcgacac agattaacgg caatgtgaat
     2641 ttgaatcaga atgcggcatt ggtcttaggc aaggctgcat tatgggggca aattcaaggg
     2701 caaggaaaca gcagcgtcag cctaaatcaa catagcaaat ggcatttgac cggcgacagc
     2761 caagtacaca atctgtcatt ggcggatagc catattcatt tgaacaacgc ttccgatgcg
     2821 caaagtgcaa ataaatacca cacgctcaaa atcaatcatt tatccggtaa cgggcatttt
     2881 cattatctga cgcacctggc gaaaaatctt ggggataaag tgcttgtgaa ggaatccgca
     2941 tccggtcatt atcagctcca tgttcaagat aaaacaggcg aacccaatca ggaagggctg
     3001 aatctctttg atgcatcatc cgtacaagac cgttcgcacc tttctgtttc cttggcgaat
     3061 aaccatgtag atttaggcgc attgcgttat acaatcaaaa cagaaaacgg tattacccga
     3121 ttgtacaatc cttatgccga gaaccgccgc cgagtcaaac cggccccgtc tcctgccaca
     3181 aacacggctt ctcaagcaca aaaggcaaca caaacggacg gtgcacaaat tgccaagcct
     3241 caaaatatcg tcgtcgcacc gcctagcccg caggcaaatc aagccgaaga agcaaaacgc
     3301 caacaagcaa aagcggagca agtgaagcgt cagcaagcag aggcagaaaa agtcgcacat
     3361 caaaaggctg aagaagcgaa acgtcagcaa gatgcccttg cccgccaaca agcagagcaa
     3421 gaacgccaac gattggaagc cgaacgccaa gctgccgaaa ttgccaaaca aaaagcagaa
     3481 gccgaagaag cgaaacgccg agccgctgaa attgccgagc aaaaagcagc tgccgaagaa
     3541 gcaaaacgcc aagctgccga acttgcacgc caacaagaag aagcacgaaa agctgcagag
     3601 ttggctgcta agcaaaaagc ggaaactgaa cgcaaagcag cagaaattgc cgagcaaaaa
     3661 gcggaagccg aacgtgaagc cgccgaactt gccaagcaaa aggcggaaga agaaggtcgc
     3721 caagcagcac aatctcaacc caaacgtcgt aaccgcaggg ctataccacc ggaactgtca
     3781 tctgatgcaa caacccgagc tttgcctagg atagcgagaa attcaaatcc tgatgccagc
     3841 gattatgaag aaatcccctt ggatgcgttg gaagatgaag atgtatccga atcggttgac
     3901 acatcagaca aacagcctca agacaatacg gaacttcatg aaaaagttga gacagtgagt
     3961 ttgcaaccaa gagccgctca gccgcgagcc caagccgccg cgcagccgca agcccaagcc
     4021 gtcgcgcaag ccgatgcagt cagcaccaat accaactcgg ctttatctga cgcaatggca
     4081 agcacgcaat ctatcttgtt ggatacaggt gcttcattaa cacggcacat tgcacaaaaa
     4141 tcacgtgctg atgccgaaaa aaacagcgtt tggatgtcga acatcggtta tggccgtgat
     4201 tatgcttccg cacaatatcg ccggtttagt tcgaaacgca cgcaaacaca aatcggcatt
     4261 gaccgcagct tgtccgaaaa tatgcagata ggcggcgtat tgacttactc tgacagtcag
     4321 catacttttg atcaggcgag cggcaaaaat acttttgtgc aagccaacct ttatggtaag
     4381 tattatttaa atgatgcttg gtatgtggcc ggcgatattg gtgcaggcag cttgagaagc
     4441 cggttacaaa cgcagcaaaa agcaaacttt aaccgagcaa gcatccaaac cggccttact
     4501 ttgggcaata cgctgaaaat caatcaattc gagattgtcc ccagtgcggg gatccgttac
     4561 agccgcctgt catctgcaga ttacaagttg ggtaacgaca gtgttaaagt aagttctatg
     4621 tcagtgaaaa cactgacggc cggactggat tttgcttatc ggtttaaagt cggcaacctt
     4681 accgtaaaac ccttgttatc tgcagcttac tttgccaatt atggcaaagg cggcgtgaat
     4741 gtgggcggta attccttcgc ctataaagca gataatcaac agaaatattc agcaggtgcc
     4801 gcgttactgt accgtaatgt tacattaaac gtaaatggca gtattacaaa aggaaaacaa
     4861 ttggaaaaac aaaaatccgg acaaattaaa atacagattc gtttctaaaa tactaaattc
     4921 atagcaaaat aaaatgccgt ctgaactcag gcttc
//
LOCUS       DAWKQJ010000136         4928 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000136 DAWKQJ010000000
VERSION     DAWKQJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.136"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            18..289
                     /locus_tag="WJL20_001648"
                     /pseudo
     CDS             18..289
                     /locus_tag="WJL20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            329..1363
                     /locus_tag="WJL20_001649"
     CDS             329..1363
                     /locus_tag="WJL20_001649"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ7683780.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1569..2150
                     /locus_tag="WJL20_001650"
     CDS             1569..2150
                     /locus_tag="WJL20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ7683781.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPAGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQLGKRHQK"
     gene            2219..2401
                     /locus_tag="WJL20_001651"
     CDS             2219..2401
                     /locus_tag="WJL20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ7683782.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALGEEELKV"
     gene            2398..3159
                     /gene="kdsB"
                     /locus_tag="WJL20_001652"
     CDS             2398..3159
                     /gene="kdsB"
                     /locus_tag="WJL20_001652"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7683783.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAAFEVV"
     gene            3297..3956
                     /locus_tag="WJL20_001653"
     CDS             3297..3956
                     /locus_tag="WJL20_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683784.1"
                     /translation="MKRNILIFLSCIALPVCAADFSAYVPKGWKIIKSVEGDLNGDNQ
                     ADAVLVLQKQDKANIIPNDSMGLPELDTNPRMLKVLFKQAGGYKTAVENTTLIPPESD
                     KDNGCLDDPFSDVSIAKGRLKVSLQWFYSCGSWWTGRSDYLFRYQNDRFLLIGYTNFQ
                     YNRASGEIEEYSDNYLTGKRKRMTGGNVFDSSEDRPKVKWERFKKKPYHLDEPYQHPT
                     D"
     gene            4054..4557
                     /locus_tag="WJL20_001654"
     CDS             4054..4557
                     /locus_tag="WJL20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7683785.1"
                     /translation="MTDLHHLSREEQKLLAEVWGLVQNDDQEVHYEMLKLNAPDEASG
                     EFWFRMAETLSTLPPNRSLDLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKITALNY
                     LAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAALARFPEDAWFQEIKQ
                     DAQKHFA"
     gene            4714..4806
                     /locus_tag="WJL20_001655"
     tRNA            4714..4806
                     /locus_tag="WJL20_001655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4748..4750,aa:Ser,seq:gct)
BASE COUNT         1269 a         1184 c         1322 g         1153 t
ORIGIN      
        1 atgaaagata ctggaaaatg aaagagatga aacctgtccg ttatcatatt ggcgatatgc
       61 ccgagacttc aaaacaaacc gcctcccgtc atgacgacag ggcagtgggt gttgacgatg
      121 attgttttca tgattccttt ggtcaatttt tgtttgggtg ttcggcagag gcaacccgaa
      181 ccgcgccaat ttctgtaaag cgcagttgct tatttacctg attggttcgc ttatcggttt
      241 ggtcttcgcg ttgtttatag gtgggtctgt atcgggtacg catgattaat gccccgggct
      301 gattttgctt cgaggatttg tatcgaatat gccgaattgt ttcaaatttc ataccgttat
      361 cgaacggcat tggcaaaaac cttatccggt tttgtctttt ctgcttaagc cgctctccgg
      421 gctgtttgcc aaaattgcgg caaaacggcg gacggatttt ttatcgggaa aacggcaaag
      481 cgaaaagctg cccgtgcctg tggtcgtggt cggcaatatt cacgcgggtg ggacggggaa
      541 aacgccgatt gttgccgcgc tggtgtcggg tttgcaggaa aagggcgtca aggtcggcat
      601 catcagccgg ggctacgggc gcaagagcaa ggcggttcat gtattgaatg ctgagagccg
      661 agcggaagat gcgggcgatg agcctttgct gctgttccgc aaaaccggtg cgccgacggc
      721 ggtgggcagc agccgtgcag aggcaggcag ggcgttgctg gcggcgcatc ccgacatcgg
      781 actgattgtg gcggacgacg gtttgcagca ttacgccctg cggcgagatg tggaaatcgc
      841 ggtgtttccg gcggcggata cggggcgcac ggatttggat ttactgccca acggcagttt
      901 gcgcgaacct ttgttgcggc tggattcggt ggatgcggtc gtcgtcagcg gcggcaaggc
      961 ggatgcgctg tttaggccgt ctgaaaatat gtttcacagc cgtatcgaag cgggacggat
     1021 ttaccgtttg aacaatccgt ccgaaatact ggacacaggc cgtctgaaaa atcaaaccgt
     1081 cgtcgccgtg gcaggtattg ccaagccggc gcgttttttc gattcgttgc ggaatatggg
     1141 cattaccgtg aagcgaaccg tcgcgctgcc cgaccacgcc gacatttcgg cggcagattt
     1201 gcccgatgcg gacgcggtca ttattacgga gaaagatgcg gtcaaatttt cagacggcat
     1261 ttgcaccgat aatgtttggg tgttgcccgt ttgtgcgata atcgaacctg atttggcggc
     1321 gtttgtgttg gagcggttgg aagatgtacc gaaggccgtc tgaaagcacg gtttgggcgg
     1381 agtgattacg gatttgaata agaacgcctc gcgccgtcat tcccgcgcag gcgggaatct
     1441 aagtctcgaa ttttcaggaa tgcctaggag gcttcagaaa tcccaaatct ccggatttcc
     1501 acttggacag gaatgagaaa atcggtcgta ttttttatct gcattaatca ttcgttaaag
     1561 tattgaatat taaactgaaa accttgttat tgcccttcgc cacgctggca ttgtgcacca
     1621 acgcttttgc cgccccgccc agcgacgcgt cgttggcgcg ttggctggat acgcagaatt
     1681 ttgaccggga tatagaaaaa aatatgattg agggctttaa tgccggattt aaaccgtatg
     1741 cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca aaagccttta
     1801 accgctatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa caggctgtcc
     1861 gcaatacttt attgaagaat gcccgtgaga tatacacgca agaagaaatt gacggcatga
     1921 ttgcctttta cggttcgccc gccggtcagt ccgtcgttgc caaaaatccg cgcttaatca
     1981 agaaatcgat gagtgaaata gcggtatctt ggactgcatt gtcagggaaa atcgcgcgac
     2041 atcatctgcc cgagtttacg gaagagttgc gacgcatcat ctgcggcggt aaaaatcccg
     2101 atgcgggctg caagcaggcc ggacagcttg ggaaaaggca tcagaaataa atgatagccg
     2161 tctgaaatat tgcagagggc atccgattga ttaaaccatc aaacccgaaa gcaaccttat
     2221 ggaaaaaaaa ttcttagaca tcctcgtctg ccccgttacc aaaggcaggc tggaatatca
     2281 tcaggacaaa caggaattgt ggagccgtca ggcgaagctg gcgtatccga ttaaagacgg
     2341 catcccctat atgctggaaa acgaagcgcg agcgttgggc gaagaggagc tcaaagtatg
     2401 accgaattcg tcgtattgat tccggcgcgg ctggattcgt cgcgcctgcc cggaaaagcc
     2461 ttggcggaca tccacggcaa accgatggtc gtgcgcgttg ccgaacaggc ggcaaaaagt
     2521 aaagctgtgc gcgtcgtcgt tgccaccgac catcccgata ttcagacggc ctgtcaggcg
     2581 cacggcgtcg aagtcgtcat gacttcaaac cggcacgaaa gcggcacgac gcgccttgcc
     2641 gaagcctctg ccgcgctgaa gctgccgccg catctggtgg ttgtcaacgt acagggcgac
     2701 gagccgctga ttgcacctga actcatcgac cgcaccgccg aagtactcgt cgaaaacaac
     2761 gtccaaatgg cgaccgccgc ccacgaattg cacgatttcg acgaattgat gaatcccaat
     2821 gccgtcaaag ttgtcctcga caaaaaccgc aacgccatct acttcagccg cgccccgatt
     2881 ccctatccgc gcgatgcgat gcgtgcagga aaacgcgaaa tgccgtttga aaccgccgtc
     2941 ctgcgccata tcggcatcta cgcctaccgc gtcggcttcc tgcaacgcta caccgaaatg
     3001 agcgtttcgc cgctggaaac cattgaatcg ctggagcaac tgcgcgtcct gtggcacggc
     3061 tatccgatcg ccgtcgaaac cgccaaagaa gcccccgccg ccggcgtgga tacgcaggaa
     3121 gatttggaca gggtcagagc cgcgttcgag gtcgtctgaa aacgagctca gcgagtttcg
     3181 ctaaaacaag cgaagcagat ttcgccaaaa tccgaaatca gtcggcttcg gcattagatt
     3241 tcacgagcca agcctacgct acggactatt tgaattttct accatcagga attgctatga
     3301 aacgtaacat attgatattt ttatcctgta tcgccctgcc tgtttgcgcc gccgattttt
     3361 ccgcgtacgt tcccaaaggc tggaaaatca tcaaaagcgt ggagggggat ttaaacggcg
     3421 acaatcaagc cgatgccgtt ttggtgctgc aaaagcaaga caaggcaaac atcatcccaa
     3481 acgactctat gggactacct gaattggata ctaatccgag gatgttgaaa gtattgttca
     3541 agcaggcagg cggttacaaa acggctgtcg aaaacacaac gctgattccg cctgaaagtg
     3601 ataaagataa tggctgtctt gacgatccgt tttctgatgt atccatcgct aaaggtcgtc
     3661 tgaaagtgag tttgcagtgg ttttacagtt gcggaagctg gtggacgggt cgcagtgact
     3721 acctattccg ttaccaaaac gaccgctttc ttctgattgg gtataccaat ttccaatata
     3781 acagagcttc aggcgaaatt gaggagtaca gcgacaatta tctgactgga aagcggaaga
     3841 gaatgactgg cggcaatgtg ttcgattcgt cagaagaccg tccgaaagtc aaatgggaaa
     3901 gatttaaaaa gaaaccttat catttggacg agccttatca acatccaaca gattgagata
     3961 ggtcgtctga aaatgatgag ccgtaaaaag ctgttttcag taaacaccaa atccgataat
     4021 ccgttctacc caaacaccga cagaaagccc tttatgaccg atttacacca tttgagccgc
     4081 gaggagcaaa aactgcttgc cgaagtatgg ggtctcgtgc aaaacgacga tcaggaggtt
     4141 cattatgaaa tgctcaaatt gaacgcaccc gacgaagcta gcggcgaatt ttggttcaga
     4201 atggcagaaa cactcagcac cctgccgccc aaccgttccc tcgaccttag aatgaacggc
     4261 ggcaggctgg cgaccgccgt atccatccta tcggtgatga ttgaggacaa tcccgacata
     4321 ccgcagcttt gggcgcagaa aattaccgcc ctcaactatt tggcacacgg acacaaagcc
     4381 cgtgccgacg gtttggcaca acagcccgac aaagcggcag aagccaacga ggaggaatac
     4441 ctgaccaaag ccctgtcgca aaacctgctg tcaacattgg atgccgcgct tgcacgtttt
     4501 cctgaagacg cgtggtttca ggaaatcaaa caggatgcac aaaagcattt tgcttgagga
     4561 tgtggcagtc aggaatattt ccattcagga agaaaagaag tgcctgattg ggtataatca
     4621 gggtaaatct tattttattt caaaagatta atatttgctt tctgtttttc cttgacggta
     4681 tcggaaaagt tgattatagt tacagcttcc ttaggagtaa tggctgagag gctgaaggca
     4741 cttccctgct aaggaagcat gtggggtcaa cctgcatcga gggttcgaat ccctcttact
     4801 ccgccagata aaaaatagac gctgtgtttt acagcgtcta ttttttatgc aattttatag
     4861 cgggttggtg caaaaccagt atggtattgc cctgtcttga ttctgaattt tgttatagtg
     4921 gattaaca
//
LOCUS       DAWKQJ010000137         4922 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000137 DAWKQJ010000000
VERSION     DAWKQJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.137"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(651..1187)
                     /locus_tag="WJL20_001656"
     CDS             complement(651..1187)
                     /locus_tag="WJL20_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ7683786.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVLLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1221..1577)
                     /gene="folB"
                     /locus_tag="WJL20_001657"
     CDS             complement(1221..1577)
                     /gene="folB"
                     /locus_tag="WJL20_001657"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ7683787.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1633..2235
                     /gene="plsY"
                     /locus_tag="WJL20_001658"
     CDS             1633..2235
                     /gene="plsY"
                     /locus_tag="WJL20_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ7683788.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIWATLLIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            2312..3157
                     /locus_tag="WJL20_001659"
     CDS             2312..3157
                     /locus_tag="WJL20_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ7683789.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3403..4377
                     /locus_tag="WJL20_001660"
     CDS             3403..4377
                     /locus_tag="WJL20_001660"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ7683790.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4520..4738)
                     /locus_tag="WJL20_001661"
     CDS             complement(4520..4738)
                     /locus_tag="WJL20_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ7683791.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1284 a         1466 c         1005 g         1167 t
ORIGIN      
        1 aagtatccat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
       61 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
      121 atagatatca tatcatttcc ttatcttcca taaatcggct tcggttgttt aaataccgcc
      181 cgtgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
      241 cttggcagga taaacctacc aaggcttttt attaaaaaaa acgctctatt actgtgtcta
      301 aaaatgcaaa aaaagtaacc atcataaccc ttttgccaaa atcaatatca tcatccgatg
      361 aatgaattga cggtatataa gaaagaatta ataagctcat aaaccggtaa gaaaaatcag
      421 taacaaagat attcattatt aactattctt aaaaatgatc acgttcgttt gggtaattat
      481 cagcccatac cataccctac tcctattact aaagcacgaa tgactttaaa gggcgcaccc
      541 gacggcaagc cgaacccttg cctccccgaa gccgaaatca gaaaaaccat accccatccg
      601 ccaaacaaac tgcccgccga tctactccgg cgggcagttc caacatcctg tcaatccttc
      661 atcaaccagt attgcaaacc gattaatgtt ttaccgtctt taatttcatc gtttgccaat
      721 gcctgacgga cttcctcctt cgacatcaat acggtttccg taatctcgtc ttcgtcattg
      781 gcaagcgtac tgcccaaacg cacgccttcc gcttcgaaca gatacatttt ttcgttgcaa
      841 aaaccgaccg ccgtataaaa actgtaaagc aggagtacgc tgtcggcggt ataaggtgtt
      901 tcctccgcca attctcgcag cgcacacgct gccatatcct cgcccgccac atccagcttg
      961 cccgcaggaa gttccaatgt cgcctgattt gccgcataac gccactgccg caccaaaacg
     1021 accttttcat catccgttac cgccaacacg cacgccgcac ccggatgacg gatgacgata
     1081 cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatact gacgaaaccg
     1141 ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc atatctcctt
     1201 tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc cacgccaacc
     1261 tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca caccgcgccg
     1321 aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag caggaaatcc
     1381 tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac cgtattggca
     1441 atatcgtcgt ccgaacccgc tttctcggga acaccgatgt ccaaatcgac aatcagggtc
     1501 tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt cataccgtgc
     1561 aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac cattctaagt
     1621 aaagtaccgc aaatgttcaa catatcggct gttgccgttt cctatctgat cggctcactt
     1681 tctttcgccg tcatcgtgtc caaatattac ggcatggacg acccgcgcac ctacggatcg
     1741 ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc cgcgctgacg
     1801 ctcttgggcg atgccgccaa aggtttagtt gccgttttgc ttgcacgcgt gcttcaagaa
     1861 ccgctcggtt tatccgacag cgcaatcgcg gccgtcgcac tcgccgcgct ggtcgggcat
     1921 atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc attgggcgtg
     1981 cttctggcac tctctcctgc aactgccttg gtctgcgcgt tgatttggct tgtgatggca
     2041 ttcggcttca aagtatcctc ccttgccgca ttaaccgcca caatcgccgc cccccttgcc
     2101 gcactgtttt ttatgccgca tacttcttgg atttgggcga cgctccttat cgccctgctg
     2161 gtgttgttcc gccacaaaag taatatcgtc aagctgctcg aaggcagaga aagcaaaatc
     2221 ggcggcagcc gctgatttcc catccatccc tgtttatgcc gtctgaaccc cgcttcagac
     2281 ggcatccgaa tatcccgccg ttttaccgac tatgctcagt tacagacacg cattccacgc
     2341 cggcaaccac gccgatatgc tcaaacactt caccttgttt ttagtgctgc aatatttcaa
     2401 ccgcaaagac aagccctact ggtacatcga cacgcacggc ggcgcgggtg tgtacaatct
     2461 cgaaggcagc gaggcacaaa aagtcggcga ataccggcaa ggcatcgccc tacttcgaca
     2521 agcaaaaaac ctgccaaccg agttaaacgg ctttatcgcg cacatacaaa aaatcctgcc
     2581 ctcgcccgaa ctttactgcg gttctccgtg gctggcgcaa tcgctgaccc gcgtcggcga
     2641 caaattacgc ctgtttgagc tgcaccccac cgactttgtc catctgcaaa acaatatggg
     2701 cgaagcagga ttgggcaaac gcggacaagt gttgcgcgaa gacggttaca aagggctgat
     2761 ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc atcgacccgc cctatgagga
     2821 aaaacaggac taccggcgtg taacggaaac gctgaaagcc gctttaaaac ggtttgagtc
     2881 cggctgttat ctcatttggt atccctgcct cagccgcgaa gaaagccgca agctgcccga
     2941 agaattgaaa aaactcctgc ccgacaacta ccttcacgcc gaacttcacg tccacgcgcc
     3001 gaaagccgac ggtttcggta tgcacggcag cggaatgttc gtcatcaatc cgccctatct
     3061 gttggcggaa caattggcgg caaacctccc cgccctgacg cggctgctgg cgcaagacga
     3121 aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc cgatgccgtc tgaaaccctt
     3181 cagacaacat ctcccgtcaa ataaacaaaa ccggacgata taccggcagt cggcactttt
     3241 aagaataaat atcccctcat ccgcctgccg cgttccgact tgccccatgc cgttttttta
     3301 cactacaatc ccctagacta tcctgcaaaa cgcaccaaag gaatacgcca caataacctg
     3361 ccgcctttca gacggcacaa acctttccga aacgagcaaa ctatggacac actgacccga
     3421 ttcctccccg aacacctgca acaaaaccag ctgcccgaag cactcggcgg cgttttactg
     3481 tccgttgttt ccgcctgcac cgaaatcaac gccaaagtcc gcctcggcgc actggccggc
     3541 gtattgggta tggcgggcac gggcaatatt cagggcgaag accaaaaaaa actggatgtt
     3601 atcgccaaca acatcatgat tgacacactc aaagccaacc ctgccgttgc cggtttggca
     3661 agcgaggaag aagactcttt cgtaagtgcc ggtgaaaacg gacgctatct cgtcctattc
     3721 gaccctttag acggatcgtc caatattgat gtcaacattt ccgtcggtac aattttctcc
     3781 atccttgaaa aacccgaagg cgcattggca accgaatcat tccttcaaac gggcagacag
     3841 cagcttgccg caggctatat cctctacggg cctcaaaccc agctcgtatt cacattcgga
     3901 cacggcgtat atatgttcac gctcaatgcc gaaaacgaat ttgtgctgac caaagaaaaa
     3961 ccgaaagtac ccgaaagtac caaagaattt gccatcaata tgtccaaccg ccgccactgg
     4021 ctgccccccg tccaacaata catcgacgag ctcttggcgg gcgaaaccgg cacgcgcggc
     4081 aaaaactaca atatgcgctg ggtagccagc atggttgccg aaatccaccg catcctgatg
     4141 cgcggcggcg tgttcatgta tccgcaagac aaacgcgacc cgtccaaacc cggcaaactg
     4201 cgcctgatgt acgaagccaa ccctatgagc ctgatactcg aacaggcggg cgcatcggca
     4261 agcaacgcat atcaagccat gctcgacata cagcccgaaa acctgcacca acgcgtcgcc
     4321 gtcttcatgg gcagcagcga agaagtcgcc tatctcgacc gcctgcacgc aaaatagaca
     4381 gacagccgtt cacacaagta cacggcggcg gcaatccgat accgccgccg ttatttcaaa
     4441 cagcacaagc cttctggtca aatgacaaaa atgccgtccg aacacatttc agacggcatt
     4501 tcaaaaactc aaacccgaat tattttcccg ctgctttttg caggatggcg tacaactcgt
     4561 ctttcagttt caatttggct tttttcagct catcgatggt ttccgcaccg ctggtaaccg
     4621 gattgttgac cagaccggta attttatcgt ccagctcgtt gtgttcgtcg aacagacggg
     4681 cgaagcggga attttcctgt ttcaatttgg aaatcaaatc acggtattct gggaacatat
     4741 cgctttcctc tcttggttta cggttaaaaa gtcaaactga atctttacat taattataac
     4801 aaagataata tttttatcca gtgttttatc ggatacggca aaattttctt ttaacaccgc
     4861 cgtccggttg ataaaaacgt tgacaatatc acgctttacg cataaatttt cagcctttcc
     4921 ta
//
LOCUS       DAWKQJ010000138         4921 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000138 DAWKQJ010000000
VERSION     DAWKQJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.138"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1161..1598)
                     /gene="rnhA"
                     /locus_tag="WJL20_001662"
     CDS             complement(1161..1598)
                     /gene="rnhA"
                     /locus_tag="WJL20_001662"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ7683792.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1711..2580
                     /gene="tehB"
                     /locus_tag="WJL20_001663"
     CDS             1711..2580
                     /gene="tehB"
                     /locus_tag="WJL20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ7683793.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEILLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVSAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2630..3436)
                     /gene="thiD"
                     /locus_tag="WJL20_001664"
     CDS             complement(2630..3436)
                     /gene="thiD"
                     /locus_tag="WJL20_001664"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ7683794.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            3497..3694
                     /locus_tag="WJL20_001665"
     CDS             3497..3694
                     /locus_tag="WJL20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683795.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(4738..4813)
                     /locus_tag="WJL20_001666"
     tRNA            complement(4738..4813)
                     /locus_tag="WJL20_001666"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4777..4779),aa:Glu,seq:ttc)
BASE COUNT         1094 a         1214 c         1372 g         1241 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccactcggac atctgtcctt cggggcggca ggatcagact ttatttggga
      121 gggggtgtaa ccccttccaa atcaggacga cacataggga ggtgctttat gtgtcgtcct
      181 gtgtgttgaa atatatcgga cgcgtttgaa cagttgtgcg gcaaactgtt ttagcggcag
      241 gcaagagatg ccgtctgatg ccgtctgaag cccattgtcc gtcattccca cacaggcagt
      301 tgctacggtt gctgaagccc cttagccgtc gcgcaggcgg ttgctacggt tactaaaatc
      361 ccgtcattcc cgcgcaggcg ggaatccagt ccgttcggtt tcagttgttt ctgataaatc
      421 ttgcagcgtt gggtttttag attcccgctt tcgcgggaat gacggggcgg tcggatgccg
      481 cctgaaactc cgtcattctt gcaacttttc gtcattcccg tgaaaacggg aatctagaac
      541 ttcaaacttt cagataatct ttgaatattg ccgttgcccg acggtctaga ttcccgcttg
      601 cgcgggaatg acggtagagc ggtttcagtt gctcccgata aatgccgcaa tctcaaatcc
      661 cgtcattccc tcaaaaacag aaaaacaaaa tcagaaacct aaaatcccgt cattcccgca
      721 gctcttcgtc attcccgtga aagcgggaat ctagaccttt taaattttca cacggctttt
      781 gaatattgcc gttacccgaa ggtctggatt cccgcctgca cgggaatgac ggcagagcgg
      841 tttcagttgt ttctaaataa atttttgcag cgttggattt ttagattccc gctttcgcgg
      901 gaatgacggg gcggtcggat gccgcctgaa gcccgacaaa gcccccaatg ccgtctgaaa
      961 cctcgtcatt cctctgctgt ccgcaacttt tcgtcattcc cgcaaaagcg ggaatctaga
     1021 gcctctaaat tttcagacgg cttttgaata ttgccgttgc ccgacggttt ggattcccgc
     1081 ctgcgcggga atgacggtgg aggacgatgc cgtctaaagc ccattagcgg tttcagacgg
     1141 catttttgcc ggagcggcag tcaggaaaac tgcgctgcgc cacggtttgc caaatcgtcg
     1201 gcgcgttcgt tttccgcgtg tcccgcgtgt cctttaaccc aagtccagct gacttggtgc
     1261 tgcccaacca gaacgtcgag ttctttccac aagtcgtcgt ttttgacggg ctgtttggcg
     1321 gcggttttcc agccgttgcg cttccaaccg tgtatccagt tttccatgcc gtttttgacg
     1381 tattgcgagt cggtgcagat gatgacggtg cagcggcgtt tgagcgattt cagtccttcg
     1441 atgacggcgg tcagctccat gcggttgttg gtggtttgcg cctcgccgcc gaaaagttct
     1501 ttttcgtggc tgccgtagcg cattaatacg ccccagccgc ccgcgccggg attgcctttg
     1561 cacgcgccgt cggtgtaaag gtaaacgggt gtgtccatat tgtgcctttg tgtggtgtcc
     1621 ggcgtgatta taagcggttt gcgcgccgtg ccgtctgaaa gattgcggcg gattgaaata
     1681 ggcatataat gtgcaacttt atttttgaat atgaaagagc ggatagtggg tcaaagcggc
     1741 gagttgtttt gtttcggaca aatgcctgta tggaaagtgg aaaacctgcc ggaaattttg
     1801 ttgtcgggct attcgtctga ggaaggggaa tgggtctgcc tgaatgtgtt gcagggcgat
     1861 gtcgaagtcc gtgcaccgga cgggtcggca gaggtttggt cggcagacgg cggcgattgt
     1921 gtgtttgcgc cgcagcaggt gttttcggtc aaaccgaaaa cggacgatgc cgaaatccgc
     1981 ttgtcgatgt attgcacggc ggcagactat tttcacaaaa aatacgggat gagtgccacg
     2041 cattctgcag tgtcggcggc tcaggatacc gtacctgcgg gcagggcgct cgatatgggc
     2101 tgcggacagg gacgcaacgc gctgtttctc ggtttgaagg ggtttgaagt tactgccgcc
     2161 gattgcaatc ccgccgcctt ggcaaacgtg gcggagctgg cagaggcgga gggtttgaac
     2221 gtccgcacgc tggaatatga tttgaacgcc gccgccttgc agggtgagtt tgattatatt
     2281 gtggcaacag tggtgctgat gttcctgatg ccgcagcgcg tgcctgacgt gattgccgat
     2341 atgcaggcgc atacggcggc gggcgggtac aatttgattg tatcggcaat ggatacggcg
     2401 gatttcccct gcccgatgcc gttccctttt aaatttaaag agggagagct gaaggattat
     2461 tatcgggatt gggagctggt cgaatataaa gaagaattgg gcgcgatgca cgccaaagac
     2521 gaaaacggca atccgatacg gtttaaattt gtaacgatgc tggcgaaaaa gcctgaataa
     2581 tcctcaaaaa tgccgtctga acattttgtt tcagacggca tttttacggt tagtcccgat
     2641 aggcccaatg attgaccggg ccgtgtcccg cgccgatttc caaagggctt gagattgccg
     2701 ccgtgatgta ggacttggca gtccgtacgg ctttgcaaac gtctaagcct tttgccaact
     2761 cggcggtaat gcaggcagaa aacgtgcagc ccgtgccgtg cgtgtgggcg gtcggaaagc
     2821 gcgggctgtc caattccagc gtttcgtttt gcgtaaacag ccaatccgtg cagcgtccgc
     2881 tgctgctgcc attcaaatgt ccgcctttga tgacgacatt tttgacaccg taatcaagca
     2941 ggatttttgc cgcacgttcc gcatctttac ggttttcgat atgcacgccg gtcagggctt
     3001 ccgcctcggg caggttgggg gtcaatacat ccgtatcggg aagcagcagg cgcgtcagtg
     3061 ccgcaacggc ggaatcctgc aacagcggcg caccgccttt ggcaatcatc acagggtcga
     3121 gtacgcgcct gccgaagtcg cagtgtttca gcttgtcggc aacgcattcg atgatttccg
     3181 ccgtgccgag cattcctatt ttgtaggcgc ggatgtcaaa gtcttccctg attgcttgga
     3241 tttgtgcggt gatggtttcg gtcgggacga gatgaaccgc cgacacgccc aaggtatttt
     3301 gcgcggtaac ggcggtgatg acgcacgttc cgaacacgcc gcgcatctga aacgttttca
     3361 aatccgcctg aatgcccgca ccgccgcccg aatccgaacc ggcgatggta agcgtttgca
     3421 caaaagagct tttcatttgt tcctccttaa tattgtgtcc gataaaaagg gctaatgcct
     3481 gtaaaaaagc acggcgatgc cgtctgaaaa ccgcagcgcg ggggtttgtg gtgcattata
     3541 tgccgtattg caggacaagg cacagggcag ggcggcgggg tgcatcgtag caactgtatt
     3601 tttgcccgac ggggtgaaaa atacagttgc tacacccact gataatgaaa aaagagagca
     3661 atgccgtctg aaccgcgtgt tgcggcgagc ataaaccggc acgctttatt cgtcatttcc
     3721 acaaaaacag aaacctaaaa ttccgtcatt cctacgaaag cgggaatcca gtgtgttcgg
     3781 tttcagttgt ttttgggttt cgggtgattt ccaaatcatc attcccgcgc aggcgggaat
     3841 ctagcccttg gggcagcggc aatatttata aagccgtctg aaaattcaga agttctagat
     3901 tcccgttttc acgggaatga cgaagggttg tgggaatgac gaagtttcag acggcatcgc
     3961 cccgccccgt cattcccgca taagcgggaa tccaaaccat tgggcagagc agccttaaag
     4021 gttataaaag actcgtcatt cccacgaaag tgggaatcca gacctgtcgg cacggaaact
     4081 tatcggataa aacggtttct tcaaccctac gctctagatt cccgcttttg cgggaatgac
     4141 gaagtttcag acggcatcaa atgctttgtc aggcttcaga cagcatcgtc caccctctgc
     4201 cgtccttccc gcgcaggcgg gaatccaatg tgttcggttt cggttgtttt tgggtttcgg
     4261 gtaatttcca aatcgtcatt cccacgaaag tgggaatcca gaccattggg cggcggaaat
     4321 attcaaaagt cgtctgaaaa ttcagaggtt ctagattccc gttttcacgg gaatgacgaa
     4381 gggttgtggg aatgacgaag ttttagacgg cattgaatac tttgatgctt tggtgggctg
     4441 aagcccaccc tacaatatag agaaaatgcc gccaaacggt atggaagcgg aaacaatggt
     4501 ggggctggct tcagaaacaa tggtagggtg ggcttcagcc caccgcttta gacggcatcc
     4561 gcgcaccgtc attcccgcgc aggcgggaat ccagccttgt cggcacggaa acttatcgga
     4621 taaaacggtt tcttcaaccc tacgctctag attcccgttt tcacgggaat gacgaagagt
     4681 gtgagaatga cggttcataa ataaaaaaac ctgcttaaaa caagcaggtt tttgaattgg
     4741 cacgcccacg gggattcgaa ccccggttgc cgccgtgaaa gggcagtgtc ctaggcctct
     4801 agacgatggg cgcgtgggaa tgggcattat acatacttct agaaatttag caatacgatg
     4861 ccgtctgaaa cttcgtcatt cccgcgaaag cgggaatcta gaatctccaa gttttcagac
     4921 a
//
LOCUS       DAWKQJ010000139         4900 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000139 DAWKQJ010000000
VERSION     DAWKQJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.139"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(247..975)
                     /gene="rmpM"
                     /locus_tag="WJL20_001667"
     CDS             complement(247..975)
                     /gene="rmpM"
                     /locus_tag="WJL20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ7683796.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1339..2289
                     /locus_tag="WJL20_001668"
     CDS             1339..2289
                     /locus_tag="WJL20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ7683797.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2402..3136)
                     /gene="fnr"
                     /locus_tag="WJL20_001669"
     CDS             complement(2402..3136)
                     /gene="fnr"
                     /locus_tag="WJL20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ7683798.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3341..4762
                     /gene="hemN"
                     /locus_tag="WJL20_001670"
     CDS             3341..4762
                     /gene="hemN"
                     /locus_tag="WJL20_001670"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ7683799.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1253 a         1340 c         1184 g         1123 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc cgaatccgaa
       61 tccgaatccg aacctgcacc ccgattctga cgatagccgt ttcagaaaac cacccaaaaa
      121 aaacaaaccc cgcaaaagcg ggggtttgtt tcacatcatc gatgccgacc gacgtttcac
      181 gaatattgca acagtacctg gtacaaaatc cactaacctc atgcatcggc aagatattgc
      241 ctagccttag tgttggtgat gattgtgtgc cggcacaacc tgacgggtta cgatgctgcg
      301 gattttcaca tccacacggc ggtcaggttc gatacatgca atcagagcct cacgtttttt
      361 ggctttagag actttcgcac ccagtttggc aacttcggct tcacaaactt gagtcatttg
      421 cgcttgagat tcgcccaagc cgacagcaga aattctagaa acaggtacgc cgttgctgac
      481 caggttgttt gccactacgt atgcgcggcg ttcggacagg gcctgattgt atttgtcaga
      541 acccataaag tcggtatggc cttcgacgcg gacagattgg atattggttt gacccaggcg
      601 ttgcgccaat actttcaggt tgtcttgagc ttcggcgcgc aatgaatcct tatcgaaacc
      661 gaacagggtt ttggcagaca gggaaatggt ttcatcaaca tattgcggag cctgctccac
      721 aacgacgaca ggagcgggtg cgggttcggg ttctggctcg ggttcggggg cagcaaccgc
      781 atcgccgcat tctacgcgac cttggcttgc tttatcaaag taggcgtttt tccagcattc
      841 gccatagttg ttgcgtacga tttcgttcga ctggccgctt acggtgtaac cctgaacgga
      901 cgcctcgccc gcaacagcag tgccggaagc gagcaatgca acgaataatg cgcttaattt
      961 cagctgtttg gtcattttat tccctcatta aatttgtaca gcagatacag aagcaactgc
     1021 tgaattgcca acatacaggc cgctgtagaa acatggcggc aaatcggata tttcagtctc
     1081 cactataaag aagcaggggg aggactgtca atactcaacg cctgaaaaca accgtaaaac
     1141 agcctgtttc ctgatagtct atcatgccga aaaatcaatt ccgtgtcatt actttagggg
     1201 gattttttcc atatcgcacg attgccgttg cacaaaaaca acaaacacaa cggcaaagcc
     1261 ccataccgta ccggcaagaa aatatgataa attataaaca atgttcagcc acccgacaca
     1321 gacccacacc gacccgccat gaaattacaa caattgaaat acgccttaga agtttaccag
     1381 cacaacctga acgtttccga agcggccgaa gccttgttta cttcgcaacc cggcatctcc
     1441 aaacaaatca aattgctgga agaagaaatc ggcattcaga tttttatccg cagcggcaag
     1501 cgcgtggttt cggtctcgca gccgggcaag gtggttttgg atattgcgga acgtatttta
     1561 cgcgatgtac agaacattaa aaatatcggc agcgagttta ccggacagga cagcggttcg
     1621 ctgacggttg ccacgacgca tactcaggca cgctatgccc tacccttgat tgttgccgat
     1681 tttgtgaaac gctatccgaa agtcaacctg accatcaaac aggggagccc tgccgccatc
     1741 gcacgtatgg ttacctcagg cgaggcggac ttggcgattg ttacggaacg gatagacgac
     1801 catcccgaac tgggaaaact tccctgctat gactggactc atgcggttat cgtaccgaac
     1861 gaccacccct tgctcgaatg cagaaacccc ctccgcattg aagatttggc gaggtttccg
     1921 ctgattactt atgaatttgc attcaatgcg ggcagcagca tcgcgcgggc attttccaaa
     1981 gcccgtttgg aacagccgga tgtcgcattg gcggcggcgg atacggacgt attgaagact
     2041 tatgtgcgct tgggtttggg cgtgggactg atggcgaaaa tggcgtacaa cccggatacg
     2101 gacggcgatt tgcagcttgt ggatgcggca cacctgttcg agccgtcgcc gacgtggatt
     2161 gctttgcgca gcgatactta tttgcgcgga tatgcctacg actttatcca agcgtttgcg
     2221 ccgcacctga cacgcgagaa ggtggatagg attctgtaca cgcccatcag cgaggatttt
     2281 tcgatttagg cggctgccgg ttttcagacg gcactttgcg gcagatacaa caaacaggac
     2341 agatgttttc gtctgccctg tgtttattga gaatgccgtc tgaaatgttc gtacgggtta
     2401 atcaaatggc gtgcgaacaa ccggatacca tcttcttcaa cacctgcaga ttgaggatct
     2461 tgatgtgttt gtgttcgacg gaaatcaatc cttcctgatg aaatttagat aatgtgcggc
     2521 tgacggtttc aagtttcagc ccgagataac tgccgatttc ttcgcgggac attcttaaga
     2581 tgaagtcgtt ggcagcaaaa cctcgggaat aaaggcgttg ggaaaggttc aacagaaagg
     2641 cggcaatccg ctcttcggcg cgcatattgc ccaacagcag cataacacct tggtcgcgca
     2701 cgatttcacg actcatcatg cggaaaaagt gggtacgcag gctggggata ttctgcccca
     2761 gttcttcgat gtgggtaaac ggcagttcgc acacttcgct gtcttccaag gcgaccgcgt
     2821 cgcaactgtg cacatgggaa cagatgccgt ccatgccgat gagttcgccc gacataaaga
     2881 aacccgttac ctgatcgcgg ccgtcctgac tggcgacggt tgttttgaag aagcccgaac
     2941 ggatggcaaa gagcgaggta aaggcttcgc cgacacagaa caggtattcg ccctttttca
     3001 ggcggcggct ttgacggatg acggcatcga gttggctgag ctcgttgggc agcagcccga
     3061 caggcaggca gagttcccgc aaagaacagg aagaacacag cgttttcatc tgatgtgtag
     3121 tattatgcga agccataccg tacctttttg tgcgctttgc cccatcatga ttatagtgga
     3181 ttaaatttaa accagtacgg cgttgcctcg ccttgtcctt atttaaattt aatccactat
     3241 atgtgcttat tgacacatat caagacaggt ttatcatact gtggcattct accaaactat
     3301 ccaaaatttg actaacatca taaaaccgca cacacacacc atgaaaatca ttcagataca
     3361 gaacaatcac aatgtaaacg atgaccgccc cgagtttgac cgcgcgctga ttgccagcct
     3421 gcccgccagc ggcccgcgct acacttccta ccctaccgcc gaccgtttcc atgacggttt
     3481 ccgcgaaggc gaatatatca aagctttaca tttgcgcggt atgggcgcgt taaacaaacc
     3541 gctttccctt tacattcaca ttccgttctg caacaccatc tgctactact gcggctgcaa
     3601 caaaatcatc accaaagaca aaagccgcgc cgatgcctac atcgaatatc ttgaaaaaga
     3661 aatggaactg ctcgctccac atctgaacgg acggcaccag cttgcccaac tgcacttcgg
     3721 cggcggcacg ccgacctttt tgagcgacga acagatcgaa cgtgtcttcc gcatgatacg
     3781 caaacatttc gagttaatcc ccaccggcga atactccatc gaaatcgacc cgcgcaaagt
     3841 cagccgcgac accgtcctca tgctcggcag actcggcttc aaccgcatga gcatcggcat
     3901 tcaggatttc gaccccaaag tgcaggcagc ggtcaaccgc atccaaagtt acgaagaaac
     3961 caaagaagtc atcgatgcgg cgcgcgaagc ggggttcaaa tccgtcagcg tcgatttgat
     4021 ttacggcctg ccgcaccaga cttcggaaag catcaaaacc accatcgata ccgttttgtc
     4081 gctcgatccc gaccgcctcg ccctttatca ctacgcccac ctgccgcacg tgttcaaacc
     4141 gcaacgccgc atcgataccg cctccgtccc cgacagcgaa gagaagctcg atatgctgca
     4201 atactgcgtc caaaccctaa ccgaacgcgg ctacgtcttc atcggcatgg atcatttcgc
     4261 caaacctgac gacgaactct ccatcgccct caaagaaggc ttcctccagc gcaacttcca
     4321 aggctattcg acctacgcgg attgcgattt ggtcgccatc ggcgtgtcgt ccatcggcaa
     4381 aatcggcagc acctattccc aaaacgaacg cgacatcgat gcctactatg ccgccatcga
     4441 cgaaggcaga ctgcccatca tgcgcggtta tcagctcaat caggacgaca tcctgcgccg
     4501 caatatcatt caggatttga tgtgccgctt cgcgctcgac taccggattt acgaaagtat
     4561 gttcggcatc ccgttcgacc gctacttcaa agacgaactg gcggatttgg aaaaactcgc
     4621 cggtttggga ttggtgcgcc tgaacagcca cgggctgacc gttaccccga aaggacgctt
     4681 cctcatccgc aacatcgcca tggtgttcga ctaccacctg cgccacaagg aaaccaaggc
     4741 gaaatattcg caaacggtgt gattgcgtct aacgtacaaa tgccgtctga aaggcttttt
     4801 cagacggcat tttgctgccg gcaggataag tgttttcaag aacaggcggc ggcatatcat
     4861 aacgttccgc acctttgtgt ccgaccgttc cgaaaccaag
//
LOCUS       DAWKQJ010000140         4897 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000140 DAWKQJ010000000
VERSION     DAWKQJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.140"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..382)
                     /locus_tag="WJL20_001671"
                     /pseudo
     CDS             complement(1..382)
                     /locus_tag="WJL20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1390..2160)
                     /gene="xth"
                     /locus_tag="WJL20_001672"
     CDS             complement(1390..2160)
                     /gene="xth"
                     /locus_tag="WJL20_001672"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7683800.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMVEALKDVRVDLETRALERPSDHAPVAAEFDW"
     gene            complement(2214..2501)
                     /locus_tag="WJL20_001673"
     CDS             complement(2214..2501)
                     /locus_tag="WJL20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ7683801.1"
                     /translation="MNTILLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2558..2836
                     /locus_tag="WJL20_001674"
     CDS             2558..2836
                     /locus_tag="WJL20_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683802.1"
                     /translation="MIVIIFINHIINQIFMDDCTGLYVPFPTFFANTRKGGLSDGIKL
                     PPLPCLTLLPNGIIRIVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2969..3850)
                     /gene="nadC"
                     /locus_tag="WJL20_001675"
     CDS             complement(2969..3850)
                     /gene="nadC"
                     /locus_tag="WJL20_001675"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ7683803.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3926..4864)
                     /locus_tag="WJL20_001676"
     CDS             complement(3926..4864)
                     /locus_tag="WJL20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683804.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1114 a         1239 c         1304 g         1240 t
ORIGIN      
        1 ggcgttggta gaaatcccga ataagcggtt caaaacgtgt cgctgccacg gtagccatat
       61 acagcgcctt acgtaccgca gactttccgc caaagcagcg gcttttgaat ttggtttccc
      121 cgctctccct cgggtgcggg gcaatgccgg ccaaactcgc tatccgtttg tgcgacagcc
      181 gccccaattc gggcagcatc gccatcagcg tagccgtcgt tatcgaaccg atgcctttga
      241 tttgctccgc cacttgggct ttgccgtcaa aatgcgtgtg ggtgtggtcg tcgatttgtt
      301 tgtccaattc gtcaatcagc cggtcaaaat gggcaatcag ttgtttgacg cttccgactt
      361 gcgtttcatg aaactgatgc agatatagtg gattaacaaa aatcaggaca aggcgacgaa
      421 gccgcagaca gtacaaatag tacggcaacg ctgtactggt ttaaatttaa tccactatat
      481 tttgtaatag ctgccattat gcctactagg atatatacct tctatataat ccttatttaa
      541 ataaaaaccc ttataaaaat ctgtgttatc tgtcgggatt gaaaaaaaga ttttatcttt
      601 ctcatcaggt ttatatttgc tatcaatatc aaaaagagaa taaggattac aatgagttcg
      661 acatactagg taaaaattta tatcttggat catgaaatat tcgatattta tcaggatcga
      721 aagatgctaa aaattcatca atattggtac tggatatttt gtcatcacta ataaacatcg
      781 ctagagatgg gcgactttga ttttccattt gttgattagt aaaggatata aatactaact
      841 tgccattgtc aaaccacata gatttatata gtggattaaa tttaaaccag tacgtcgttg
      901 cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
      961 taatccacta tagttattgc ctttgggaga atcaattctg acagcacata acataggttc
     1021 atctttacag tcgttatatt gggtttcatc aaactcaact gcatagcaca ataaaggcaa
     1081 taggaaaaaa aggagattaa tttattacta tacataaggc agtctgacta ttcattctaa
     1141 aataaatccg gtttttaata tttgattctg ctccggatta tatacataat aaattttatt
     1201 gctccaaaat ctgatattaa ccctatggat ttcataaatt tttcagaaaa ataggctgtg
     1261 cttgaacaat ccgccgactc cggctgtttg tcagggaaag gcaattattt ttcggtcagg
     1321 cagaaagtta tcctattgaa tcacttaaag atatggatgt ttgtcaacgc catatcaaaa
     1381 cacggtcttt taccaatcga attctgccgc cacgggcgcg tggtcgctcg gacgttccag
     1441 cgcgcgcgtc tccaaatcga cgcggacatc cttcaacgcc tccaccatcg caggcgacac
     1501 caaaatatgg tcgatacgca ggcccagttt gcgttggaac atcgcgccgc gatagtcgaa
     1561 ccaggtatag aacgcgcctt cgggatggac ttggcgcagg ctgtcggtca gtcccaaatc
     1621 cagcaggttt tgaaaccact gccgttcgac ggacgaacag tggatttttt cgtgccattt
     1681 ttcagggtcg taacagtccg catcggcagg cgcgatattg aaatcgccca gcagcaccag
     1741 tttgccgtag cgggtcattt catcgcggac aaactccgtc agtgcggcaa accattgttc
     1801 tttatatttg aatttcgggc tgtcgagggc ttcgccgttg acgcaataga cattgataac
     1861 gcgcacaccg ccgacggttg ccgcaatcac gcgccgttgc ggatcgtccg gcagcgcggg
     1921 cagcccgata tgcacgtctt ccggcgcgtt gcggctgacg attgccacgc cgttgtaggt
     1981 tttctgcccg ctccaaacac agtgccagcc catcatttgc aaagcggcgg ccggaaattt
     2041 gtcctgatcg agtttgagtt cctgcaaaac caaaatatcg ggcggattgt cggcaagcag
     2101 gttttgcacc tgcggcagcc gcacattgag cgaattgacg ttccaagtgg tgattttcat
     2161 aacatttccg aaaaatgccg tctgaaacgt tcagacggca aaaggaaagg attttaacac
     2221 cataccgcgc gtttgttttc caaatcgcgg attgtccgca gaatcgttgc cgcgtcttcg
     2281 gaaatcaggc ggtattcgat aatgcggtgg taacgcgtaa agtccaccag accgcccgcc
     2341 ctcaggcggt tcaaatggcc ggaaacggcg gtggccggca gggataagga ttttgccagt
     2401 tcggcgatat tgcgttcgct gtccaacagt tggatcagta tcgccatacg ttcgggatgc
     2461 gccataagtt tgagtatggt gtggagcagt attgtgttca tcggtattcc taaaaccttt
     2521 gccgcccgcc aagcggattg acgggcgaca gatatggatg attgtgatta tctttataaa
     2581 tcatattata aatcaaatat ttatggatga ttgtacagga ttgtatgttc cttttcccac
     2641 tttttttgca aacacgcgta aaggcgggct ttcagacggc ataaaactgc cgcccctacc
     2701 ctgcctcacc ctgttgccga acggaatcat ccgcattgtt caaaagatgc cttacaaccg
     2761 ccgcacgtct tgccctgccg atgcctccgt gccttcgggc aaacaaaata cctgcctgaa
     2821 acgctgtttt ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca
     2881 gacggcattt cctccttgag gcaataaccg gattacggtt aggcggttcc ttgtctgaca
     2941 tatcagacaa ctgccgacac cctaaaactc atgccaagaa atccaaacct atgtccaacg
     3001 agcggctgct gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc
     3061 gcttcaggcg gtcgaagccg atgccgcccg atgcttcgca atagacggtg tgggggtggg
     3121 cggtttgcgt gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca
     3181 gcaaaatccg ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaac gtgtccactt
     3241 cgatttccac gcaggtcaat gctccgaccg cctgttttgc ctgccgaacg gcttgggcga
     3301 tgctgccgca ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aagcccatgc
     3361 ggtggttcac gccgccgcct gccctgacgg cgtatttttg caggacgcgc agcaggggga
     3421 tggttttgcg gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcacgcg
     3481 cggtggcggt ggcgatgccg cttaagtgcg tgaggtagtt gagtgcggtg cgttcggcgg
     3541 cgagcagcgc gcaggcgttg ccttcgacga cggccagcgt ctgacctgcg cggacggctt
     3601 gtccgtcttg gatttcggct tggaaacgga cggacggatc catcgtctga aaggcgagac
     3661 acgccaagtc cataccggcg ataacaccgt cttcgcggct gacgaggaag agtttggcgg
     3721 ttttgtcggg ggcgataacg gcggcggacg taatgtcgcc gcgcctgccc aagtcttcgc
     3781 tcaaggcttg ttcgactatg gggcgcaaca gggtgtcggg cagggaaaag agggtgtttt
     3841 cagacggcat gaggtttcct ttgtcgaaaa agattgatgt tgcaccgatg gggcaaaact
     3901 tcatttgatg ccgtctgaaa acgggctaac ggctgcccag cggcagtccg atgtccgaaa
     3961 tcagcctgtc gggcaggacg tcgtcgcgga agcggcaaag ctgcgcggga cggcctttgc
     4021 tgcccgatac gccggtatcc gacggctcga tgaggttttg ctgctgaatc tggcggcgga
     4081 agttttgctt gtgcagcaat cttccgctga tggcttcgac actgttttgc agttgcagca
     4141 gcgtgaattc gggcggcatc agctcaaaaa tcacggggcg gtatttgatt ttggcgcgca
     4201 ggcgggacag ggcggtcgcc aatacgcggc ggtggtcgtg gcgcatgggc tgccctgtga
     4261 gcgcgaaatc gaagtttgcc tgcggctcgg cggcttccac tatcaggccg ctttcataca
     4321 gcatttcata gcgttgcaaa acgtattctt ccgaccagtt ttccggttcg accccccaac
     4381 acaaatgaat gcgcttgagc cgcatttgac gcacttcctc cgtgtccgcc gagtttgccc
     4441 aaatgcgcag tcggctgacg acggcgtcgc gctgtccgcc gtcggtgcgc aagtcttccc
     4501 acgggaaata gccgtagcag tcctgccatt tcgcatcggg atgcaggatg ctgtcggctg
     4561 cctcgcgcac cagccccaaa tagctgacgt acaagacggg catgccgtgt tcgttgcggc
     4621 ggtgggtatc gacaaaggtg taaagctgtt ccacatagcc cataggctgc gaagtctgct
     4681 tggcaaccca cagcttcacg cccgcctgca aggaattgcg cagaggggag agcgggccgt
     4741 tgggcaggag tgtgccttgg gcgacggtta atacccgcag gccgccgtcg gtaacggcaa
     4801 tcaatacggg aaccagctcg acgatgcttt gcggcggggc ttcggcttcg gggtaggcat
     4861 ccatgctcgg ctttctgcgg gtgggaaagg ctttatt
//
LOCUS       DAWKQJ010000141         4877 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000141 DAWKQJ010000000
VERSION     DAWKQJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.141"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            158..964
                     /locus_tag="WJL20_001677"
     CDS             158..964
                     /locus_tag="WJL20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ7683805.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKTDFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(1022..2287)
                     /locus_tag="WJL20_001678"
     CDS             complement(1022..2287)
                     /locus_tag="WJL20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ7683806.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITSE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2694..3404
                     /locus_tag="WJL20_001679"
     CDS             2694..3404
                     /locus_tag="WJL20_001679"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ7683807.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3477..3938
                     /gene="recX"
                     /locus_tag="WJL20_001680"
     CDS             3477..3938
                     /gene="recX"
                     /locus_tag="WJL20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ7683808.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            4012..4173
                     /locus_tag="WJL20_001681"
     CDS             4012..4173
                     /locus_tag="WJL20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683809.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            4326..4808
                     /locus_tag="WJL20_001682"
     CDS             4326..4808
                     /locus_tag="WJL20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7683810.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1273 a         1435 c         1113 g         1056 t
ORIGIN      
        1 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactataca
       61 aaaagacagt tttcagacag caaatccgtc ttcacacgat acctattttg ttataacata
      121 acaaaatctt taacccacac gagacaaagg ctgcaccatg aagaaaacat tgacactgct
      181 cgccgtttcc gccctatttg ccacatccgc ccacgcccac cgcgtctggg tcgaaaccgc
      241 ccacacgcac ggcggcgaat accttaaagc cgacttgggc tacggcgaat ttcccgaact
      301 cgaacccatc gccaaagacc gcctgcacat cttcagcaaa ccgatgcagc tggttaccga
      361 aaaaggcaag gaaaacatga ttcaacgcgg cacatacaac taccagtacc gaagcaaccg
      421 tcccgttaag gacggcagtt acctcgtcat cgccgaatat cagcctactt tctggtcaaa
      481 aaacaaagca ggctggaaac aggcgggcat caaagaaatg cctgacgcaa gctattgcga
      541 acaaacccga atgttcggca aaaacatcgt caacgtcgga cacgaaagcg cggacaccgc
      601 catcatcacc aaaccggtcg gacaaaactt ggaaatcgtc ccgctggaca atcccgccaa
      661 cattcacgta ggcgaacgct tcaaagtccg cgttctgttc cgtggcgaac cgctgcccaa
      721 tgccaccgtt accgccacct ttgacggctt cgacaccagc gaccgcagca aaacgcacaa
      781 aaccgaagca caggctttct ccgacagcac agacgacaaa ggcgaagtgg acatcatccc
      841 cttgcgccaa ggcttctgga aagccaatgt cgaacacaaa accgacttcc ccgatcaaag
      901 cgtgtgccaa aaacaggcga actactcgac tttaaccttc caaatcggtc attcgcacca
      961 ttaatcccgc ccgcacaaaa atgccgtctg aaggcttcag acggcatttt ttgttcaaac
     1021 atcaatacca accgcgcagt ttcatcgctt tttcaacacg gcggatactc atcatgtaag
     1081 acgcggttcg caaatcgaca tcatactctt gcgccaagtt ccatatatcg cggaacgcgc
     1141 gtcgcaggac gacggtttct ttctcttgaa cttcgtcaaa ctcccaataa tagccttgca
     1201 ggttttgcac ccactcgaaa taggaaacga ccacgccgcc gcagttcgcc agaatatcag
     1261 gcacgaccaa tacgccgttt tgacgcagga tcacgtcggc ttcgggcgta gtcgggccgt
     1321 tcgcgccttc gactacgatt ttcgcgcgga ctttaccggc gttttcggaa gtcagttggt
     1381 tttccagcgc gcaaggggcg agtacgtcca catccaaagc caaaagttcg gcgttggtaa
     1441 tttccttgcc gtaaccggct tcgctggtga tgaagccttt ttcttggaac tctttaaaca
     1501 aagcttccat atccaagccg ttttcgttgt aaatggcaac gtcaacagta gaaaccgcaa
     1561 caactttcgc gccggattga tgcgcgtaat aacctgtgtg gtaaccgaca ttaccgaaac
     1621 cttgaatggc gtaagtggca cccttcacgt ccttgcccag tttttccaag gcttggacgg
     1681 cggcgaggtt cacgccgtaa cccgtggctt cggtacgcgc caaagagccg ccgaactcga
     1741 ccggtttgcc ggtaaatacg cccggtgcgg aatgtttcac cacgttttca taagcatcca
     1801 ccatccacga cataattttg ccgttggtgt tcacatcggg ggcgggaata tcgatttttt
     1861 cgccgatcag cggagaaatg gcttcggaat aagcgcgggc gatgcgttcc agttccgcct
     1921 cggaataatc gcgcggatcc aaggtaacgc cgcctttgcc gccgccgtaa ggaatgccgg
     1981 ccacgcagca tttgatggtc atccaaatcg acagggcttt gacttcgtcc agattcacac
     2041 cgggatggaa gcgcacgccg cctttatagg ggccgacggc gttgttgtgt tgcgaacggt
     2101 agcccgtgaa ggttttgacc gtgtcgtcgt cgagtttgac gggaaaattg acttccaaca
     2161 cgcgggtcgg actcttcagg atttcataaa cggccggatc ggttttcagc cggtcgcagg
     2221 cggttttgac ctgtttgcgc gcgatttcaa acggattgag ggtttctttt gcaagggctt
     2281 cagacatttt gcttcctttt cacaaaaaga ttttcagggg cgtgcatttc gctcattact
     2341 ataaccaatt tcccgacaaa atgtaatagc gtgtagtcgg cataagcccg atttgattaa
     2401 tctatatatg attttatttt tcaagccgca cggaatcagc ccggactccg cctgaaaaag
     2461 cagcgcgcgc atccgaaaaa taaatgtcaa attaaataaa gatataggca tccttttatt
     2521 ttttaaaaat ttaattggaa catcgctggg atttcaaacc cttgcccgat tccgccccga
     2581 aatccgaaaa caccgcctgc aaaacctgtt gcgattgtta acaatctata cattagaagc
     2641 cctgtgcaaa cgatgttaaa ataaaccctt tcaacccgac aggaaatcag attatgaatg
     2701 cagccatcga acacgtccaa gccgtcgcct tcgatttgga cggcacactg tgcgattccg
     2761 tccccgacct tgccgccgcc gcagaagcga tgttggaaca actcggtatg aaaccgctgc
     2821 ccgccaaagt ggtcgaaagc tatgtgggcg acggcatcgg caaactggtt caccgcgtcc
     2881 tctccaacga ccgcgaccgc gaagccgatt ccgaactgtg ggaaaaaggt ttcgtatcct
     2941 atatgaaata ctaccgcgac catttgagtg tcttcacccg cccctatccc gaaaccgaag
     3001 ccgggctggc attgctcaaa tctttgggca tcccgctggc agtcattacc aataaaaacg
     3061 aaatccttgc cgccgaactg ttgaaacagc tcggacttgc cgactacttc agcctgatac
     3121 tcggcggcga cagcctgccc gaaaaaaaac ccagccccct gccgctgcgg cacgccgccg
     3181 aagttttggg tatcgatgtt gcaaacatgg ttatggtcgg cgactcgcgc aacgacatca
     3241 tcgccgccaa agccgccggt tgcctgagtg tcggcgttac cttcggttac ggcgatatga
     3301 cgctgctctc gcaagacgat gcgacccgcc ccgactggat tatcggctcg ctgcccgaaa
     3361 tttacgaaaa cctgcaaccg cagaaaaata aagacgaaga ataaaacaat ctgttcagac
     3421 ggcatcggaa tccgtttccc atgccgtctg aatctttccg tgccgaaacc gccgccatga
     3481 aaccgcaaaa atccctacgc gcccgcgcga tggacatcct ctcgcgccaa gaagtcagcc
     3541 gcatcggtct gaaacgcaaa cttgcaccgc acgccgaaag cgaagaggag ttggaaaacg
     3601 tgttaaacga atttgccgaa cgcaactggc agtcggattt gcgctatgcc gaagcctata
     3661 tccgcagcaa aagccgcaaa cacggttcat tgaggctgaa acaggctttg gcgcaacagg
     3721 gcatagatga agaaaccagc cgcaacctgc ttcccgaccg ctcaagcgaa aaacaggccg
     3781 ccatagccgt gttgcgtaaa aaattcaaac accccgccgc cgacctcaaa gaaaaacaaa
     3841 aacaggcgcg tttcctcgcc tatcgcggat tcgatgccga taccgttcag acggcattga
     3901 aacacgcttg ggacgaaaat tgggaagacg gctgctgacc ttccttcttg aatctttttg
     3961 cacggcaaca taaccttacg cccgtttcca acttttccga ttgagaacaa aatgtccgga
     4021 caacccgaaa aaaatcataa ccccctcctt gaggacgaac gcaagaaccc cgtgtaccgc
     4081 atggggcagg cagttgccgg attcatgctc gtcgtttggg caggcgtatt ggcactcgtg
     4141 tttttcctgg tcttccgttt ttggctttcc taaacaaaat gccgtctgaa ggcttcagac
     4201 ggcatcggcg gcccatttct gcagactatc ccgccatagc tttttttagc ttgaattcca
     4261 cttccccatt ccctaaaatt ttcccacacc catttcaaaa caccctttct taaaacaggt
     4321 acactatgac acaacaacgc caactgcctt cccacgaact cattatgtcc gaactgatga
     4381 tgccggacac cgccaatttc agcggcaacg tacacggcgg cgaactcctg ctcctgctcg
     4441 accaagtcgc ctattcctgc gccagccgtt acagcggcaa ttattgcgtt accctgtcgg
     4501 ttgacaaagt cctgtttaaa gaacccatcc acatcggcga tttggttacc ttttacgccg
     4561 ctgtcaacta cacgggacgc acctcgatgg aaatcggcat ccgtgtcgaa gcacaaaaca
     4621 tccgcaccgg cgaaatccgc cataccaaca gctgctactt cacgatggtt gcggtcaaag
     4681 acggcaaacc cgtccctgtc cctccgctgg aaatcctgac cgaccgccaa cgctgccgct
     4741 acgaaaaagc caaaaaacgc agagacatca gcctgcaagc ctctgaagac atgtcctgcg
     4801 gctgctgacg gcggactatg ccgtctgaaa gacaggcaca tcgcgccatc cgtttccatt
     4861 gcaaacggat gaaatca
//
LOCUS       DAWKQJ010000142         4707 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000142 DAWKQJ010000000
VERSION     DAWKQJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.142"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            48..452
                     /locus_tag="WJL20_001683"
     CDS             48..452
                     /locus_tag="WJL20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ7683811.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            492..1676
                     /locus_tag="WJL20_001684"
     CDS             492..1676
                     /locus_tag="WJL20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ7683812.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDIYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(1739..2272)
                     /locus_tag="WJL20_001685"
     CDS             complement(1739..2272)
                     /locus_tag="WJL20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ7683813.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(2451..4706)
                     /locus_tag="WJL20_001686"
     CDS             complement(2451..4706)
                     /locus_tag="WJL20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ7683814.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDLKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1163 a         1320 c         1195 g         1029 t
ORIGIN      
        1 cagaaattat cctaccgttt tttaaacact ttcaggaata aggaaaaatg accgcccaac
       61 cctgccccat ctgcacggca caagatgaag atattttgct gcaaaccccc aacctccgcg
      121 tcatcgccat ccataacgac agcggttcgc ctgcattctg ccgcgtcatt tggcgtaagc
      181 acatcgccga aatgaccgac ctttcggcag cggaacgcgg cgaattgatg gaaatggtgt
      241 acaaagtcga agccgctatg cgccaagtgt tccggccggc aaaaatcaac ctcgccagct
      301 tgggcaatgt cgtgccgcac ctgcattggc atattatcgc ccgctttgaa aacgatgcgt
      361 ctttccccgc gccgatttgg gcaaaccccg tccggaaaca cggtatgacc ctgccgcaag
      421 attggacgga acagcttaaa aagctgcttt aagcccgcca atgccgtctg aaaccgtatg
      481 aaagggaaat tatgaccgaa ccgacctccc gccgccgttt tctgaaaacc tgcaccgccg
      541 ctgccggcgc ggggctgctt caggcttgcg gcacatccgc cacatccgtt ccgccccttc
      601 cctcttccca ttccgttgtg aaagcccgaa ccgtgcctct ccaaacgcca cgccgtcaaa
      661 gttcggacgg caaccttctg cgcgttgtcg cttcgtcagg atttgccgaa gacaccaacc
      721 gcgtcaacac agccttaacc cgcctttaca atgtcggttt taccgtaacc aaccaacagg
      781 cgggcagccg ccgtttccaa cggtttgccg gcacggacac gcaacgtgcc gccgatttcc
      841 aagaggtcgc ttccggccgc gtcgccacgc ctaaagtgct gatgggcttg cgcggcggtt
      901 acggtgcggc gcggattctg ccgcatatcg attttgcttc gctcggcgca aggatgcgcg
      961 aacacggcac gctgtttttc ggattcagcg acgtatgcgc cgtccagctg gcattgttgg
     1021 caaaaggcaa tatgatgagt tttgccggcc cgatggctta tagcgatttt ggcaaacccg
     1081 cccccggtgc gtttacgatg gatgccttta tcaagggtgc aacccaaaac cgcctgaccg
     1141 ttgatgttcc ttatatccaa cgcgccgatg tcgaaaccga aggcatattg tggggcggca
     1201 acttaagcgt cctcgcctcg ctcgccggca cgccttatat gcccgacatc gacggcggca
     1261 ttttgttcct cgaagatgtc ggcgaacagc cctaccgcat cgaacgtatg ctcaatacgc
     1321 tgtatctttc gggtattttg aagaaacagc gcgccatcgt gttcggcaat ttccgtatgg
     1381 aaaaaattcg agatatctat gatccgtctt atgatttttc tgccgttgcc aaccatgttt
     1441 cgcgcacggc gaaaatcccc gtgctgacgg gcttcccgtt cggacacatt gccgacaaaa
     1501 tcactttccc tctaggcgcg cacgcccgaa tccgtatgaa cggaaacagc ggttattcgg
     1561 tcgcgtttga aggctacccg acactcgatg cgtccgccct gactttggat accctgctcc
     1621 caccgccgga tttgcccatc ttccccgaaa gcggtgttgc cgatatttcg gaataaaccc
     1681 gcaaacggac aaatgccgtc tgaagccttc agacggcatt tcccaagacg gcggcagatt
     1741 acagcaatgc ccgaatatcg gcttcgattt cttcgggcgt aacactaggc gcaaaacgct
     1801 cgaccacttc gccgtcgcgg ttgacgagga atttggtaaa gttccatttg atgtcgcctt
     1861 cgtcgcgctt ctctcccaaa gctgcgagct tcaacacgaa atctttaaac agatgattgc
     1921 ctttatcttg cggtttgacg gatttcaggt aggcatacaa gggcgcggta tttgctccat
     1981 tgacttcgat tttgtcgaaa atcttaaact tcgtgccaaa cttcatcata cacacttggg
     2041 caatttctcc gctgctttcg ggagcctgtt cgcggaactg gttgcacgga aaatccaaaa
     2101 tctccaagcc ttctgcggta tattgtgcat acagcttctg caaagcctcg tattgcgggg
     2161 tcagaccgca acgcgttgcc gtgttgacaa tcagcagaac cttgccgcga tagcctgaca
     2221 aatcaaccgc attgccttcc gcatctttca tttgaaaatc gtaaataccc atttttatcc
     2281 ttatctgatg taaaccgatg ccatctgaaa cgtgcttcag acggcatgaa agcagcaatt
     2341 gtatagccga ttaaaataaa aaatccacat ccttttccat tcccgtccca atccgcaata
     2401 aaaaactgca cccgaaaacg ggtgcagttg ctcatttcat accgcaaaac ttatttgtcg
     2461 cggccgaata cgattttagt agcttggatg gcaacgcaga ttgcaccgcc gataaagacc
     2521 aagtcagctg ccgtacgtac ccaacgcaag gtatcgagga tttccatttg caggaactct
     2581 tcgctgcggg cataccacag accgtgcgtg atggaggcgt atgcctgaat cgcgccgaca
     2641 ggcagcaggc tgatggcaat cataccgacc aagccgccgt tgagcagcca gaagccccaa
     2701 gtcatcagtt tgtcgtcaaa ctgcgcgttc ggtttcaaat aacgggcaac cagcaatacg
     2761 aagcccaatg ccaagaaacc gtacacacca aacaaggcgg cgtgcgcgtg aacggcggaa
     2821 gtgttcaaac cttggatata gaacagggaa atcggcggat tgatcaggaa gccgaatacg
     2881 ccggcaccga tcatattcca gaaggcgact gcaacgaagc acatcagcgg ccaacgcagg
     2941 cgtttcgccc agtcggacaa gtgttggtaa gaccaatgct cgtatgcttc acggcccagc
     3001 aacaccagcg gcacgacttc caaagcggag aagcaggcac cgattgccat agaggcggag
     3061 gtagagccgg agaagtacag gtggtgcagc gtacccggaa cgccgcccaa cataaagatg
     3121 gcggcagcgg ccaaagtgga ggcagtagcg gtactgcggc ggacaaagcc catattgtag
     3181 aagacaaagg caaaagcggc agtggcaaat acttcaaaga agccttccac ccacaggtga
     3241 accacccacc aacgccagta ttccattacg gcaatcgggg atttttcgcc atagaacagg
     3301 cccggtgcgt agaatacgcc cacaccgacc atagaagcga caaagattgc cagcaggttt
     3361 ttgtccacgc ctttttcttt aaaggcggag atggtgcaac gcagcatcag gaacagccac
     3421 aacagcagac cgaccatcag caggagttgc cagaaacggc ccaaatcgag gtattcgtaa
     3481 ccttggtgtc cgaaccagaa attcaattcg gggggaagga tgtgcgtcaa cgcgaagaag
     3541 ttgcccgcgt aagaaccgcc gaccacgatg aagagggcga tatagaggaa gttcacgcct
     3601 gcacgttgga acttgagatc tttgccgccg ttgacaatcg gcgcgaggaa caaacctgcc
     3661 gtcaaaaagc cggttgcaat ccagaagatg gcggattgga tgtgccaagt acgggtcagg
     3721 gcgtagggga accagtcgga catttcaaag cccaacgcct cgtcaatgcc gtagaaaccc
     3781 tggccttcga cggtgtagtg cgcggtcagt ccgcccagca atacttgtac cacaaacagg
     3841 gcgaccgtca ggaagacgta tttgcccaat gctttttgcg aaggggtcag ttggattttg
     3901 gaaatcgggt cttcagacgg cacttccact tcctcgtgtt tggtcaagaa ggaataaccc
     3961 cacatcagca aaccgatgcc catcagcaga agaacaacgc tggtgaatga ccacatatag
     4021 ttttcagtgg tcggtacgtt gttgatcaaa ggctcgtgcg gccagttgtt ggtataagtg
     4081 aacgtctcgt caggacggtt ggtcgaagca gaccaagaag tccagaagaa gaagtcgaac
     4141 agtttttcac gcgcttcttg gcttggcaat gtgttgtttt tcattgcaaa gtgttcgcgg
     4201 gtggtttgca gcgcgggatc atcgccgtaa acaccgtggt aataaggcag gatgctttcg
     4261 atggctttca cgcgcgtatc gctgatgacg acgctgccgt cttctttaat acggctttgg
     4321 ttgcggtatt cgtcagccag tcgggttttc agaacggctt gttcttcagg ggaaacttca
     4381 tcgaattttt tgccgtaagt ctgttgcgcg gtcaaatcca accaagcgga caactcacga
     4441 tgcagccagt cggccgtcca gtccggagcc tgatatgcgc cgtgacccag aatcgaaccg
     4501 acttccatac cgccggtact ctgccatgca gactgacctg ccaaaatatc gtctttcgtc
     4561 atcaagactt tgccggatgc ggaaacgacc tgttcggggt aaggcggggc ttttttgtaa
     4621 acctcgctgc ccatatagcc aagaatggta aagcataccg ccagaacggc aaacagcaag
     4681 taccacagct tcttgtactg tcccatt
//
LOCUS       DAWKQJ010000143         4542 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000143 DAWKQJ010000000
VERSION     DAWKQJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.143"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            67..327
                     /locus_tag="WJL20_001687"
     CDS             67..327
                     /locus_tag="WJL20_001687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683815.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            434..958
                     /gene="nspA"
                     /locus_tag="WJL20_001688"
     CDS             434..958
                     /gene="nspA"
                     /locus_tag="WJL20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ7683816.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1094..1483
                     /locus_tag="WJL20_001689"
     CDS             1094..1483
                     /locus_tag="WJL20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7683817.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1561..2391
                     /locus_tag="WJL20_001690"
     CDS             1561..2391
                     /locus_tag="WJL20_001690"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ7683818.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2406..3863
                     /locus_tag="WJL20_001691"
     CDS             2406..3863
                     /locus_tag="WJL20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ7683819.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3904..4293)
                     /locus_tag="WJL20_001692"
     CDS             complement(3904..4293)
                     /locus_tag="WJL20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683820.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKHPEIYQRFQHFFPFVELPLL"
BASE COUNT         1084 a         1446 c         1008 g         1004 t
ORIGIN      
        1 gcgcaaacaa atcggcggcc gcccgggaaa acccgccccg aacgcgtccg gaaaatatgc
       61 ttatcgatgg aaaacgcagc cgcatccccc gccgggcgtt tcagacggca cagccgccgc
      121 cggaaatgtc cgacgcttaa ggcacagacg cacacaaaaa accgtatgcc tgcacctgca
      181 acaatccgac agataccgct gttttttcca aaccgtttgc aagtttcacc catccgccgc
      241 gtgatgccgc caccaccatt taaaggcaac gcgcgggtta acggctttgc cgtcggcaaa
      301 gcagccggat accgctacgt atcttgaagt attgaaaata ttacgatgca aaaagaaaat
      361 ttaagtataa tacagcagga ttctttaacg gattcttaac aatttttcta actgaccata
      421 aaggaaccaa aatatgaaaa aagcacttgc cacactgatt gccctcgcta tcccggccgc
      481 cgcactggcg gaaggcgcat ccggctttta cgtccaagcc gatgccgcac acgcaaaagc
      541 ctcaagctct ttaggttctg ccaaaggctt cagcccgcgc atctccgcag gctaccgcat
      601 caacgacctc cgcttcgccg tcgattacac gcgctacaaa aactataaag ccccatccac
      661 cgatttcaaa ctttacagca tcggcgcgtc cgccatttat gacttcgaca cccaatcgcc
      721 cgtcaaaccg tatctcggcg cgcgcttgag cctcaaccgc gcctccgtcg acttgggcgg
      781 cagcgacagc ttcagccaaa cctccaccgg cctcggcgta ttggcgggcg taagctatgc
      841 cgttaccccg aatgtcgatt tggatgccgg ctaccgctac aactacatcg gcaaagtcaa
      901 cactgtcaaa aacgtccgtt ccggcgaact gtccgccggt gtgcgcgtca aattctgata
      961 tgcgccttat tctgcaaacc gccgagcctt cggcggtttt gttttctgcc accgcaacta
     1021 cacaagccgg cggttttgta cgataatccc gaatgctgcg gcttctgccg ccctattttt
     1081 tgaggaatcc gaaatgtcca aaaccatcat ccacaccgac aaagcccccg ccgccatcgg
     1141 cgcgtacagc caagccgtcc gagcaggcga caccgtttac atgagcggtc aaatccccct
     1201 cgatcccgcc acgatgaccg tcgtcggcaa cggcgatttc cgcgccgaag cgcgccaagt
     1261 gttccaaaac ctgcaagccg tcgccgaagc ggcaggcggc acgctggccg acatcgtcaa
     1321 actcaacgcc tacctgaccg acttgggcaa ttttgccgtc ttcaacgaag tgatggcgga
     1381 atttattgcc gagcccttcc ccgcccgcgc cgccgtcggc gttgcctccc tgccaaaagg
     1441 cgtgcaggtc gaagccgaag ccgtcctcgt tttgaacgca taattccacg ttcggactgc
     1501 atgatatttc aaaaccatgc cgtccgaacc gcaacacttt cccacttcac aacatctgca
     1561 atggcacatt acgcaatcgg cgacatacaa ggctgtttcg acgaactgac cgcgctgctc
     1621 ggcaaaatcg gtttcaacca cggcacggac accctctggc tgacgggcga catcgtcaac
     1681 cgcggcccga aatccctcga aacgctgcaa ttctgcatcc ggcacgaaaa cagtgtgcaa
     1741 atcgtcctcg gcaaccacga cctgcacctg ctcgctgtcg gctgcggcga aggcgcgccc
     1801 aaacgcagcg acacaatcga acccatactc aaacaccccg acggaaaaaa aatgctcgac
     1861 tggctgcgtg cgcaaccgct gttgatacgc gagggcagcc gcgtgatggt acacgccggc
     1921 atcctgccgc aatggcgcat cgccaaagcc gaatcgctcg ctagagaagc cgaagccgaa
     1981 ctgcgcggca aaaaatacgt caaattcttc tccaaaatgt acggcaacaa accggccgcg
     2041 tgggacgaag gtttggaagg ctatgcccgc ctgcgcttca tcgtcaacgc cttcacgcgg
     2101 atgcgcgccc tgacctttaa aaacgaactg gatttcgact acaaatccac agtgaaaaaa
     2161 atgccgcctt acctgcgccc gtggttcaaa gcccccgacc ggcaaaacct cgaccgcacc
     2221 atcatcttcg gacactggtc ctcgctgggc tacacgaatg ccgacaacgt catctcgctg
     2281 gacaccggcg cgctgtgggg cggacagctg accgccgtca acctcgaaac ggaagaaatt
     2341 acccaagtcc aagccgccgg cggcatagac tggaaaagct tcgcaaaata acggaccgcc
     2401 ccactatgca caaaatcctg cccatcgccc acgtcctctc ccgactgggt atgctgtttt
     2461 cctttatcct gctgataccc gccgccctct cctacgcctt ttcggacggc gcgtacaccg
     2521 ccttcgccac caccgcgacc gttacccttt ccggctcgta catcgtccgg ctcgccaccc
     2581 tccggttcag gcgcgaactg cgcccgcgcg acggcttcac cctcgtcctg atgttgtggc
     2641 tggcgtttgc cgctatggcg gcgatgccga tgtacctgta tttcccgaat atgggcttta
     2701 ccgacgcatt ttttgaatcg atgtcgggac tgaccaccac cggcgcgacc gtcatccccc
     2761 acgtcgacgg gctcgccccc tccgtcaact tttggcggca tatgctcaac tggctgggcg
     2821 ggatgggcat catcgtcctt gccgtcgcca tcctgcctat gctcggcgta ggcggcacgc
     2881 agttattcaa agccgaaatc cccggcattg acaaagaaag caaaatgtcg ccgcgcattt
     2941 cccaagtggc gaaaaaactc tggttcggct acaccctgat caccatcctc gcggcagcct
     3001 gcctgcattt tgccgggatg ggctggttcg atgccgtctg tcacgcgatg gcgaccctct
     3061 cgctgggcgg gttttccacc cacgatgcca gcatagctta ttacaactcc cccctcatcg
     3121 aagcggtcat catcgttttc accattgtcg gcggcatcaa ttttgccaac catttcgccg
     3181 ccctcaacag ccgcagcctc aaaacctatt ggaaagacga agaatgccgg acgatgctgc
     3241 tgctgctctc cggcagcatc cttgccgccg ccctgtacct gtggcacacc ggctattacg
     3301 ccggttttac cgaatccctg cgctacaccg ccttcaactt cgtctccatc ggactggcaa
     3361 acgggctggc caacaccgac ttcgcacaat ggccgctcct gatttccctg tggatgtttt
     3421 tcctcgccaa catcctcgcc aactccggct cgaccggcgg cggcatcaaa accatacgcg
     3481 ccctcgtcct gttcaaattc agcctgcgcg aaatgatggt gctgctgcac cccaaagccg
     3541 tccgcaccgt caaaatcagc ggcaaggcca tacccgaccg cctcgcgctg accgttatgt
     3601 ccttcatctt catctacttt atgaccgtcg tcctcttcag cttcctgctg atggcgagcg
     3661 gtatggaatt taccaccgcc ttcaccgccg tcatcgcctg catcaccaac gccggccccg
     3721 gcctgggcga agtcgggccc gccggcaatt acgccggttt ggacgtgatg cagaaatgga
     3781 tctgcgttac cgccatgctc ttgggcagac tggaaatctt caccgtcttc atcctcttca
     3841 ctcccgccta ctggaagaaa taaaaaatgc cgtctgaaaa cttttcagac ggcatcttgt
     3901 cctttaaaga agcggaagct ctacaaaagg aaagaaatgc tgaaacctct gataaatttc
     3961 aggatgtttc tctaaggctt ttaatgcttt ttcttttgaa ataggcggat catagacatc
     4021 tatccccctt aagaaggcaa tgcccgttaa tgcatttttt tgatcatttg aaatgtaacc
     4081 ataattaacg actccaatta catcataaat ttcccctata ctcttatcaa aatcaatcaa
     4141 atcaaaaata tacaatggaa ttgtatcaat aggagtatct ctgacaacta tatccaacca
     4201 taatttaaat tcagaaagat taatagcatc atagaacata cagactattg caaatcctaa
     4261 atcttcacaa ttttctttac taattttcca cataattatt tacctttatc cattgcttcc
     4321 caaccgatac atccggtatt ttagcttctc cttttccgtc aaacactcct ttggttacct
     4381 gctttgtgtc aaaaataccg tctgaaagcc gaatatcgtt tcagacggta ttttgattgt
     4441 tgggttttta atcagagctt tgtttgataa tttgttattt tgaataaaat acaggttctg
     4501 tcgagctgat aacttctatt acagaatcgc taaaaaagaa aa
//
LOCUS       DAWKQJ010000144         4529 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000144 DAWKQJ010000000
VERSION     DAWKQJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.144"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..1851)
                     /locus_tag="WJL20_001693"
     CDS             complement(58..1851)
                     /locus_tag="WJL20_001693"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ7683821.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="WJL20_001694"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="WJL20_001694"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ7683822.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="WJL20_001695"
     CDS             3218..4480
                     /locus_tag="WJL20_001695"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ7683823.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1025 a         1249 c         1267 g          988 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagct tgctcaaagc
      121 ggaaaggtag gctttggcgg tggcgaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatc gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcacccca tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 agtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgt gcgccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc attgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgcttc
     1681 cgccaacgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacccg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcgc aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat taatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacg ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggatcgggt tcggcaaacg gcacgggcgg tatgctgacc aaaatcaaag cggcaaccat
     2761 cgccgccgaa tccggcgtac cggtgtatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagctgcc gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg gaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca
     3061 gcccctgggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagtctgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtcaagac ttggaagccg cggcaggcaa aattcccgaa agcatgaccg accgcctttt
     3421 gttggacggc aaacgcattt gcgcgatggc ggacggcatt agggcggttg ccgcactgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa
     3541 aaagcgtgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgcactcaaa gccggtttgg cgcaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacacaggg cgtgagggca gttacgaaat
     3781 gatgagggcg aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gaaaatgccg tcgtgccggt cattgaaacg gggacgggca tcgtccacat
     3901 ttatatcgac aaggacgcgg attgggacaa ggcgctccgt atcgtgtaca acgccaaaac
     3961 cagccgtccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacggctgtt ggttcgcgac cgcatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gctgtgaaaa ccgtcgcatc
     4201 ggtcgaagag gcggtcgggc acatcgaagc ccacggcaca caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg tccgatgggt ttgaaagagc tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt tagggaataa tccgaattca aacgtctgat
     4501 atagtggatt aactttaaac cgatacggc
//
LOCUS       DAWKQJ010000145         4523 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000145 DAWKQJ010000000
VERSION     DAWKQJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.145"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..1065)
                     /locus_tag="WJL20_001696"
     CDS             complement(127..1065)
                     /locus_tag="WJL20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683824.1"
                     /translation="MLELNGLCKRFGNKTVADDICLTVGRSKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVAHLMGLPNTDDDRHIPQHAVRFDQNGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1074..1949)
                     /locus_tag="WJL20_001697"
     CDS             complement(1074..1949)
                     /locus_tag="WJL20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7683825.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWTDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2138..2214)
                     /locus_tag="WJL20_001698"
     tRNA            complement(2138..2214)
                     /locus_tag="WJL20_001698"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2178..2180),aa:Met,seq:cat)
     regulatory      2364..2463
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2556..4457
                     /gene="thiC"
                     /locus_tag="WJL20_001699"
     CDS             2556..4457
                     /gene="thiC"
                     /locus_tag="WJL20_001699"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ7683826.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1086 a         1288 c         1203 g          946 t
ORIGIN      
        1 gggaatgacg gcagggtggt ttctgttgtt tcggataaat tttgcagccc tgataaaaaa
       61 tatggctgct ttggtaaaaa aatgccgtct gaaaggtttt cagacggcat ttgtttttaa
      121 gaagcatcag cggaagcgga cgatttcccg ttcttcgata tggatgcgta ccgtatcctt
      181 gcccgatacc gccccggcgt gccgcatatc gaggttcagc cataggatgc cgtgttccgg
      241 atggaggacg gacaggctga acgattcggg caaacaggta cgggataata cgcggcactc
      301 catgccgttt tggtcgaaac gcaccgcatg ttgcggaata tggcggtcat cgtcggtatt
      361 gggcagcccc atcagatggg cgacctgcac gccggcaggc gtttgaacca aggtttcggg
      421 cgtaccgcat tgaaggattt tcccctcgtg catgacggcg atttcgtctg ccgccgtgca
      481 ggcctcttcg ggcgaatgcg ttaccaaaac ggcagggatg ccgcccttgc ggatacgttc
      541 ggcagtcatg cggcgcagcc ggtcgcgcaa atgcgtgtcc aaactggaaa acgattcgtc
      601 caacagcagc agggaagggc ggacaaccaa agcgcgcgcc agtgccaacc gttgcttttc
      661 gcctccggaa agtttttcag gcttgcggtg cgcctcgttt tccagtccga cttcggcaag
      721 tgccgccatg gcgaggcttt cggcttcggc tttcggcatt ttttgcattt tcaaaccgaa
      781 tgccgcattt tccagtgcac tcatatgggg aaacagcgcg taatcttgaa acatcagcga
      841 aatacggcgt ttttcgggcg gcatacgggt aatgttttcc ccgttcagcc gaatttcgcc
      901 gccgtccgga cggataatcc ccgcaatcat attcagcagg gtggattttc cgcagcccga
      961 ccgtcccaaa acggcaagta ttttgctgcg gccgacagtc aggcagatgt cgtcggcgac
     1021 ggttttattg ccgaagcgtt tgcagagtcc gttcagttca agcatggcgt atcctataaa
     1081 cgtatgccgt ccagccactc ggacagcgga tgggcggcat caatcagacc gtaacggcaa
     1141 aacgcgtcca gcgtaaccag ttgcgcgtcg tgcatcatgt tttccgacaa catggcatcc
     1201 aacagaccgc cgatgtccat cttttcaaaa cacgcaacct cgccgtcctg attttcaggc
     1261 aggaaggttt cgggcaggat ggcatcgaat acatacagga tttcattgtg cacaccccgg
     1321 ctgacggggc gcaggctgtg cagccgagat accggacgga taaacgggaa cagcgtttta
     1381 tccaaaccgg cttcttcgct gctttcgcga cacacggttt cagacggcaa ttcaccgctg
     1441 gaaacaccgc cggcggcagt attgtcgagt ttgtcgggat cgactgcttt gtgcggactg
     1501 cgcctgccta tccagaaatg ccatcggccg tccgattcgg tcagaccgtt gagatggacg
     1561 gcgcggctga gtagtccgaa cgggcggaaa gcggcgcgtt cgagcgtgaa caaggggttg
     1621 ccgccgccgt cggtcaggtc gaaacactcg ttgcgccagc cgtgaagcag ccccgccttg
     1681 ttccatgtgc gggcgaggtg ctgcaagcgt ccgcccatat ccgtccagcc gtccgcattc
     1741 agaaaaatgc cgtctgaaga ctccgagcag cctgcctccc agtctttttt gacgcgctcc
     1801 gcccattccg gcgacagatt gcccaaaggc agacggttca gatacagcgt tttccagcaa
     1861 ctttttgcac cgtaacttgc ttttgcccac tcgaacaggg catcaaggtc ttgtttgctg
     1921 acggattcgg taaaacggac ggtcggcata gtcggctttc ttgaacataa tgcggaagat
     1981 tgtagccaaa ccgcctgaat ttgcaaccgc aaacagacgg cgggctgccg ttccgatatt
     2041 gcggcaggaa ggaaaatccg gcaaaaaacg gaggcggcag gaaacaggaa acaggcaaaa
     2101 caaaacccct cggcattgaa ccgaggggtt taatatttgg ttgcgggagc aggattcgaa
     2161 cctacgacct tcgggttatg agcccgacga gctaccatgc tgctccatcc cgcgtcagaa
     2221 gatggaacta tacggcagat tttttatgtt gtcaaacatt atttccgcga aaaatcataa
     2281 aattttgccg tccggcggtt tatgcctgat ttgaaatatc attttcttta caaaagttca
     2341 tgttcgtgat ttaattttgg ttaacattga aacaggggtg ctgcatgatg ttcaggcggc
     2401 tgagaaatac cctttacacc cgatctggat aatacctgcg tggggagttt tcacggattc
     2461 tgcctttcag acggcatttt ttgatactcg ggccgtttga aaaagcaaag tgctcctgtt
     2521 tctttattct aaacgagaaa acaggagcat tttttatgac tacgccaaaa aaaactgcca
     2581 aaacttccgg caacgaagcg cgcgaacttg ccgacttgag cgaagacatc ggcatccgct
     2641 tcaaatatcc gaactcggag cgcgtgtatc tgcaaggcag ccgcgacgac atccgcgtgc
     2701 ctttgcgcga aatccgtcag gacgacacct acacggcgca aggcacggaa gccaacccgc
     2761 cgattcccgt ctatgacacc agcggcgtgt acggcgatcc ggcggcgcac atcgacctga
     2821 aacaaggtct gccgcacatc cgcacggcgt ggctggacga acgcggtgat accgaaatcc
     2881 tgcccaagct ttccagcgaa tacggcatcg aacgcgcaca cgatccgaaa accgcccatc
     2941 tgcgcttcaa ccaaatcacc cgcccgcgcc gcgccaaagc cggccgcaac gtaacccagc
     3001 tccattacgc ccggcaaggc attatcacgc cggaaatgga atttgtcgcc atacgcgaac
     3061 gtttgaaatt agacgaattg tcccaaaaac cggaatacgc caaactcttg aagcaacacg
     3121 cggggcaaag tttcggcgcg aacatcccga cccgtcccga tcaaatcaca cccgaattcg
     3181 tgcgccggga aatcgccgcc ggacgtgcga ttattcccgc caacatcaac catcccgaac
     3241 tcgaaccgat gattatcggc cgcaacttcc gcgtcaaaat caacggcaac ttgggcaatt
     3301 ccgccgtcac ttccagcctg accgaagaag tcgaaaaaat ggtgtggtcg ctgcgttggg
     3361 gcgcggacac aattatggac ttatccacag gcgcgcacat ccatgaaacg cgcgaatgga
     3421 ttatccgcaa cgcgcccgtc cccatcggca ccgtgcctat ttatcaggct ttggaaaaaa
     3481 ccggcggcat tgccgaagat ttgacttggg atttgttccg cgacacctta atcgagcagg
     3541 cggaacaagg cgtggactat ttcaccatac acgcgggcgt gttgctgcgt tatgtaccga
     3601 tgaccgccaa ccgcctcacc ggcatcgtct cgcgcggcgg ttcgattatg gcgaaatggt
     3661 gtttggcaca tcatcgggaa aacttcctct acacgcattt cgacgaaatc tgcgaaatca
     3721 tgaaagcgta tgatgtgtcg ttcagcctcg gcgacggcct gcgccccggc tgcattgccg
     3781 atgccaacga cgaatcccaa ttcgccgagc tgcacacctt gggcgaattg accgataaag
     3841 cgtggaaaca tgacgtacaa gtcatgatcg aaggccccgg ccatgtgccg ctgcaacgcg
     3901 tcaaagaaaa catgaccgaa gagctgcaac actgctttga agcacctttt tacacactcg
     3961 gcccgctcgt taccgacatc gcacccggct acgaccacat cacctcgggc ataggcgcgg
     4021 ccaatatcgg ctggtacggc acggcgatgc tttgttacgt tacccccaaa gaacatttgg
     4081 ggctgcccga caaagaagac gtgcgcaccg gcatcatcac ctacaaactc gccgcccacg
     4141 ccgccgatct cgccaaaggc tggccgggcg cacaattacg tgacaacgcc ctgagcaagg
     4201 cgcgtttcga gttccgttgg cgcgaccaat ttcgcttaag ccttgaccct gaacgtgccg
     4261 agagcttcca cgacgaaacc ctgcctgccg aaggcgcgaa aatcgcccac ttctgctcaa
     4321 tgtgcggccc caaattctgc tcgatgaaaa tcacgcagga agtgcgcgac tacgccgaca
     4381 agcaaaaagc ccaacggcag ggcatggagg aaaaagcggt cgagttcgtc aaaaaaggcg
     4441 cgaagattta cagttgaaac gtcaagcaaa aaatgccgtc tgaaacccgg aaaaaaggct
     4501 tcagacggca ttctttcgct tgt
//
LOCUS       DAWKQJ010000146         4516 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000146 DAWKQJ010000000
VERSION     DAWKQJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.146"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            90..890
                     /locus_tag="WJL20_001700"
     CDS             90..890
                     /locus_tag="WJL20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ7683827.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1342..1980)
                     /locus_tag="WJL20_001701"
     CDS             complement(1342..1980)
                     /locus_tag="WJL20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ7683828.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            2235..3077
                     /locus_tag="WJL20_001702"
     CDS             2235..3077
                     /locus_tag="WJL20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ7683829.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            3143..3805
                     /gene="exbB"
                     /locus_tag="WJL20_001703"
     CDS             3143..3805
                     /gene="exbB"
                     /locus_tag="WJL20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ7683830.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            3808..4242
                     /locus_tag="WJL20_001704"
     CDS             3808..4242
                     /locus_tag="WJL20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ7683831.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT         1241 a          986 c         1175 g         1114 t
ORIGIN      
        1 aatttactca aaccatccaa aaattaccaa gcagcaatat aaacacagat ttatttgagc
       61 caacctgatc aaaacaagga actaaataga tgaaaaagac catttttaac attaccaaaa
      121 tggactgccc gtccgaagaa cagctcattc gcatgcgttt aaaagatgtg tctgatattt
      181 atgaattaca gtttgatatt gctgggcggt gcttaaccgt ctatcacgat aatcaagata
      241 caacgatttt acaagtactt gaaccgctta attttgatag tcatatcatt tccaccgaag
      301 tgattgttga taagatagtg tttaacaagc ctgatgagca cttagaaaaa agattattat
      361 atcaagtgct catgattaac tttgtgtttt ttatcataga gtgttcggtt ggtatttttg
      421 ctaattctat ggggctgatt gctgatagtc tagatatgct ggcggacagc tttgtctata
      481 tattggcgct atcagccatt ggtatgacgc tggcgtataa aaaacgtgtg gcatttttgg
      541 cggggataac gcagataata ttggcgttat ttggggtgat agaagtgatt aggcgtttta
      601 tcggtgctga gcagctgccc aattatcaac taatgattgg cacggcattt ttggcattga
      661 ttgcaaactg gctgtgctta tatctactga gcaaaaatca aaataaagaa atccatatca
      721 aagccagcat gatttttaca tccaatgaca ttatcatcaa tatcggtgtg atagcggcgg
      781 gagcattgac gttattgact cattctagct atcctgattt aatcattggc atgattgtct
      841 ttgtcattgt actatttggt gccagaaata ttttaaaatt ggcaaaataa ttcctttcac
      901 cagcaaagga gtttttatgc aaatatcggt caaagctacc tacatacgtg gcagtacttc
      961 caaaaacttg attttttaaa atgtggttaa tgcgagtttg tttatctggt atttgattgg
     1021 tcaagccttc atccaagcgt ttgacgattt cacgcaaaaa tacccctgat ttacacacag
     1081 ggtctaagaa tgtcgccttt ggatttgacc aaatctcaga cggcaataaa tccagcattt
     1141 gattggcaag ttttggcgat gtgaaaacct catcattaga cagactggag tttcttaatc
     1201 agtgtttcag ttaatgaaag gtattatcat gcaacttggt tctaataatg atgaaaaaaa
     1261 taagattaca ctacaggaaa acgcggcagg gcaatatgtc ctgccgcgtt ttctggagcg
     1321 tttatgccgt atgaaagcca gtcagacgaa catctgcaca aacgcaatca ccagccgcac
     1381 aatcggtgca ataaacgcac ccaaaacccc ggtcagcata agcagcagga taatccacgt
     1441 cccataaggt tcgattttgc ggaacgcttg cgaatatttc gccgacagga aggtgtcgat
     1501 gaaaatgccg ccgtcccaag gcaggatggg gatgatgttg agcgcgaaca gaatcgcatt
     1561 gatcagaata ccgtagtttg ccatttgagc caacggcatc tgatacgccc cgccgacata
     1621 cggagtcagc accaaaacca cgccccacag aacagccatc gctagattcg acagcgggcc
     1681 ggacgcggca acgcaacgcc aggcaaggcg cgggttgcgg aagttgcgcg aatcgatagg
     1741 aatcggacgc gcccagccga acaggaaggg cgtgaacatc aaagtaagca gcggtacgat
     1801 gattgtgccg accaaatcga tatggggcag ggggttcagt gtcagcctgc cgtattgttc
     1861 ggcagtgttg tctccccagt agcgcgccgt atagccgcgc gccacctccc tgacggtaat
     1921 ggcgagcagc acgggcagga cggcaagcag aaacacgccc aaatcaaaat tttgaaacat
     1981 ggttttcctt tgaacagatg tggtgtgcag cccgaaacgg ctgacagaca gaagcggcaa
     2041 tatatcatct aacctgattt tattggaata agaaatcaga cggaatcatt ttttctgccg
     2101 gcccgttatc tgatgatttg ctgtaataca gcgtttaaac acggtttgcc tgtgaacgga
     2161 acagacattc atatgaccaa aaattgtaaa aataggttgc aaataggaat tgttgctatt
     2221 attatgcccg aattatggat aaagaacgaa ttttaacccc cgcagtcgtg ttttccgtcg
     2281 cactgctgca tttggcaatg gtggcattgc tctggcaggc gcacaagctg cccgtgatag
     2341 agtcaggaaa tgttatcgaa tttgtcgatt tgggtgattt tggcggaggg gacggtgcac
     2401 ccgaaggcgc aggtgcgcct gctgcgcctg agccgcaacc tgagcctgag ccgcccaaac
     2461 ctgtcgagcc gcccaagccg gttttgaagc cggtggttac gaaaaaggcg gatgcggata
     2521 ttcagcagcc taaggaagag ccgaaacctg aagaaaagcc gaaacccgaa gaaaaaccga
     2581 aaccagagcc taagccggaa gcgaagcctg tcccgaaacc ggcggaaaaa ccggtcgaga
     2641 agccgtctga aaaacctgcc gaacatccca gcaatgcttc tgccaaagca gacagcgagc
     2701 agggtaatgg ggaagataag ggaaccggta ccaaaggaga cggaacgggg cgcggagaag
     2761 gcagcggtaa aggtagcggc ggtgtcaaag gcgaacacgg ggaaggagcc ggcagcagca
     2821 aaggcaatcc tttacgcgcc aacggcagca ttccgcgccc ggcttatccc acgctttcta
     2881 tggagaatga cgagcagggt acggttgttt tgagcgtttt ggtttctccg ggcggtcatg
     2941 tcgagtccgt taaaatcgtg aaaagcagtg gtttttctcg gttggacaat gcggcacgca
     3001 aggcggcgca aaacgggcat tttcaagcca atgcctggac ggagtttaaa gtccccgtca
     3061 agtttgaatt gaattaggcg gttgccgcag cggtttgcgg cagcttggat gtttgattgg
     3121 ttttttcagt gttggaatca ttatgaattt gaaattagta tttgaatcgg gcgatcccgt
     3181 cctgatcggt gtgtttgtgt tgatgctgtt gatgagtatc gtaacgtggt gtttggttgt
     3241 cttgcgctgc atcaagctgt atcgggcgcg caaagggaat gccgccgtca aacggcatat
     3301 gcgcgatact ttgtcgctga acgacgcggt cgaaaaagtg cgcgccgtcg atgcgccttt
     3361 gtccaaactg gcgcaagagg cattgcagtc ttaccgcaac taccgccgaa acgaagcgtc
     3421 cgaactggcg caggctttgc cgttgaacga gtatttggtc attcaaatcc gcaacagtat
     3481 ggcgcagatt atgcgccggt ttgattacgg gatgaccgcg cttgcctcca tcggcgcgac
     3541 cgcgccgttt atcgggctgt tcggcacggt ttgggggatt taccacgccc tgatcaatat
     3601 cgggcaaagc gggcagatga gtattgcggc ggttgccggc ccgattggcg aggcactggt
     3661 ggcgacggcg gcgggtttgt tcgtggcgat tccggcggtg ttggcataca acttcctcaa
     3721 tcgcggcaca aaaatactga cccaggattt ggatgcgatg gcgcacgatt tgcacgtccg
     3781 cctgcttaat caaaaggata gctgattatg gcatttggtt cgatgaattc cggcgacgat
     3841 tctccgatgt ccgacatcaa cgttacgccg ttggtggacg tgatgctggt gttgctgatt
     3901 gtgtttatga ttactatgcc ggtgctgacg cattccatcc ctttggaact gccgaccgcg
     3961 tccgagcaga caaacaagca ggacaaacag cctaaagacc ccctgcgcct gacgattgat
     4021 gcgaacggcg gctattatgt cggcggggat tctgcaagca aagtggaaat cggggaagtg
     4081 gaaagccgtc tgaaagccgc caaggagcag aatgaaaacg tgattgtggc gattgcggca
     4141 gacaaggcgg tggaatacga ttatgtaaac aaagctttag aagccgcccg tcaggcagga
     4201 atcaccaaaa tcggttttgt aaccgaaacc aaggcgcaat aaagttcgac gcttgaacga
     4261 atgccgtctg aaagtctttc agacggcatt tcttatgttt aatcagctat atatagttga
     4321 ttaaaacaaa aatgagacga cagagcaccg tctccatttc tgcgcaggcg ggaatacaaa
     4381 catctgattt ttcttttatt ttcaaacatt actgaaatgg gtaagtctgg agtcctgcct
     4441 gcgcgagaat aatgaaaatg tgcatttcta atttttatag tggattaaca aaaatcagga
     4501 caaggcaacg aagccg
//
LOCUS       DAWKQJ010000147         4462 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000147 DAWKQJ010000000
VERSION     DAWKQJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.147"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(15..90)
                     /locus_tag="WJL20_001705"
     tRNA            complement(15..90)
                     /locus_tag="WJL20_001705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Val,seq:tac)
     gene            complement(160..2136)
                     /locus_tag="WJL20_001706"
     CDS             complement(160..2136)
                     /locus_tag="WJL20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ7683832.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2285..3124
                     /locus_tag="WJL20_001707"
     CDS             2285..3124
                     /locus_tag="WJL20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ7683833.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPTFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3323..4354
                     /gene="gap"
                     /locus_tag="WJL20_001708"
     CDS             3323..4354
                     /gene="gap"
                     /locus_tag="WJL20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7683834.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1128 a         1492 c         1043 g          799 t
ORIGIN      
        1 gaaacaagga aacttggtgg gtgatgacgg agtcgaaccg ccgacattct gcttgtaagg
       61 cagacgctct accaactgag ctaatcaccc atgaattttg cgaaagcgtt attaaaccaa
      121 ataaccggaa atggcgcaag ccttatttcg gcatttcatt tatattccct gcaaactcca
      181 gttttcaaaa gagataattt cttctttttt gcctgcgacc accaacacgt caccgccttc
      241 caatgtaaac gaggcatccg ggttttcaat ccggccggtg cggcggcgga cgaacaagag
      301 tttgatgccg taagccgcca tcggaagcgt gccgactgtt ttgccgacgg cgtatgcttc
      361 tgcagccaaa ggaaaggcgt gacggacggt ttcgccgttt tcgccgaatc ctgcctcatc
      421 atcactaccg acaaacagtc cctccaacgc ggcataacgg ctgtggcgga tatttgccat
      481 cgtctgatag acgtgccggt acgacgcgcc gttgcccaac atcgcatagc ctgccagcac
      541 gagtccggtt tctttggtgt ccgacacggc ttcttccgcg cccatatcgg taaacgtttt
      601 cacataatcg tcgttggtgg cgcgcacata tacgggcata ttgggataca tggacagcac
      661 attgtctaaa acgtgttgcg tttcgtgcat attgttgagc gtaaccacca ccattttcgc
      721 ccgtcccaga ccggcggctt ccaatacttc cctgcgtttc gcatcgccga acgacaccgg
      781 ttcgcccgca cttctggcaa cctgcacccg cgcaatgtcc aagtcgagcg cgaaatacgg
      841 aatatcctct tgggcaagga cgcgtccgac cgtctgcccg cccctgccga agccgacaat
      901 cagcacatgg tcggacttgc tcatggtttc taccagcata ctgtgcagat cgagcgactt
      961 catgtcccag cttgacttga ccaaacgccc gaccagtgca tcgctgccgc ccaagaggaa
     1021 gggcgcgata atcatcgaca gcagaaccgc cgccgtcgcc gcctgttccc attctggcga
     1081 aaccatatca agctgcccgg caatggccag catcacgaag ccgaactcgc cgccctgcgc
     1141 gagatacaaa gccgttttga ggctgtcgcc gaccgaatgt ttcattttga aggcaatggc
     1201 aaacacaacc aatgccttca acaccagcag cattgccaac agcatcaata cctgccgcca
     1261 gccgccgatc aatgcctgaa tgtccagctt catgccgacc gtgataaaga aaaagccgag
     1321 caagatatcg cggaacgggc ggatgtcgtc ttcgacttgg aaacggtatt ccgtttccga
     1381 aagcagcatc cccgccacaa acgcgcccaa cgccatagac aaaccttcca gctcagtcag
     1441 ataagccaca cccaaggtta ccagcagcac attgatcata aagagttcgg acgatttgcg
     1501 ttttgccacc atcctgaacc atcgcgacat aattttgctg ccgacgaaaa acagcagccc
     1561 cagcgtcagc agcatttttg caaacgccaa acccaaggcc gcccaaatat ttccgtcccc
     1621 tccgcccgcc agcgcgggaa tcagaatcat cagcggcacg acggcgatgt cctgcatcag
     1681 cagcacgccc atcgccatct gaccgtgcgg ctgccccaat tccgtctttt ccgacaaaat
     1741 ccggctcaca atcgccgtgg acgacatcgc caacgcgccc gacacggcaa acgcccaatt
     1801 gaacggcacg cccgtcagca tcagtatgcc cattaccgac agcatcgtaa cgccgacctg
     1861 caaaccgccc agcccgaaca ccagccgcct catcgcccgc aacttgggca gcgagaactc
     1921 caaaccgatg ctgaacatca ggaacacaat cccgatttcg cccaaataat ccgtcgcatg
     1981 gcttttcgga atcaggctga gcataccggg ccccgccaaa aagcccacca gcaggtagcc
     2041 cagcatggag ggaatgttga acttgcggca caggatcacc gtaatgaccg acaccagcaa
     2101 aacaaccaca ataggggcaa gcgaaaattc gttcataggc cgtccgaaca ggaaaataca
     2161 gaaaaatgcc gtctgaaacg gcatacgccg ccgtattata acaaaacacc gcgcaccatc
     2221 cgaaacgggc gcggcataca attctgctaa aatacgccct ttcgattttg agccgcacac
     2281 cgacatgacc gccacagccg ccgccataga ccgcctgctt ccccaaaccc aatgccgcga
     2341 atgcggctac gacggctgcc tgccctacgc acaggcagtc gcaacaggcg aagcgtacaa
     2401 cctctgcgcc ccgggcggag aagccgtcgt ccgcgacctt gccgccctgc tcggcaaacc
     2461 ccttgtcgca cctgccaaaa cccaagccaa agcactcgcc cggatagacg aaaccgcctg
     2521 tatcggctgc accgcctgca tccgcgcctg ccctgccgat gccattatgg gcgcgggcaa
     2581 acttatgcac accgtcatcg ccgacgaatg caccggctgc ggactctgcg tcgccccctg
     2641 ccccgtcgac tgcatccata tgcagcccgt tgccgacacc gtcctgcccc gcgcgcgccg
     2701 cttcagcctg tccgccgaca gccgttttgc cgccgccgaa cacgcgcgcg cgcgctacct
     2761 caaacgcaac gaacgcaaac agcgcgaagc cgacgaacgc aaggccatgc ttgccgaacg
     2821 cgaagccgcc gtccgcaacg cgcgtccgca aacgcccgac acaccgaaaa aaccgacgtt
     2881 taaccctgcc gacctcatcg ccaaagccat ggcaaaagcg caaacccaac aagaccgcct
     2941 cgccgccgcc gacaaccgca aagactatca ggcgaaacag atagccgaag cccgcgaacg
     3001 cgccgagttg cgccgcgccc aacgcgatat gaaatacggc agcgacagcg aaaaagccgc
     3061 cgccctcgaa tatctcaaac aatacaaagc caaacaggaa gccgcacaga ataccgcctc
     3121 ctgacccttc cctgatatgc cgtctgaagc cgcttcagac ggcattttat caagctctcc
     3181 gtccgccgca cccgtgccgt ccgcatctta ccgcccaccc ttccagtcgc gccgttttca
     3241 ataaaatatt aattacacgc cactacaaat ttgctataat ccgccccgaa aatctaccca
     3301 accctcaaca aaggaacgaa ccatgggcat caaagtcgcc atcaacggct acggacgcat
     3361 cggccgccag gttttgcgcg ccatctacga ttatcagatt caagaccaac tccaaatcgt
     3421 cgccgtcaac gccagcggca gccttgaaac caacgcccat ctgaccaaat tcgacaccgt
     3481 acacggacgc tttgaagccg acgtatccca cgacggcggc aacctcatcg tcaacggcga
     3541 caaaatcccc ttcttctcga cccgcaaccc tgccgaactg ccgtggaaag aactcggtgt
     3601 cgatttggtc atggaatgca ccggcgcgtt caccagcaaa gaaaaagcca aagttcatct
     3661 ggaaagcggc gcaaaaaaag tcctcatttc cgcaccgggc ggcgacgatg tcgatgcaac
     3721 cgtcgtgtac ggcgtgaacg acagcgtcct gaccgccgac atgaccgtcg tttccaacgc
     3781 ttcctgcacc accaactgcc tctcgccggt tgccaaagtg ttgagcgaaa gcgtcggcat
     3841 cgtcaaaggc gcgatgacca ccatccacgc gctgaccaac gaccaaaccg ttaccgacgt
     3901 gcgccacaaa gacctgcgcc gcgcccgcag cggcgtggaa aacatgattc cgaccaaaac
     3961 cggcgcggca aaagccgtcg gtttggtact gcccgaattg aaaggcaggc tcgacgggct
     4021 tgccatccgc gtgccgaccg tcaacgtatc attggtagat ttgagcttcc aagccgcgcg
     4081 cgacacgaca gtcgaagaaa tcaacgcact gatgaaagcc gcctcggaag caggcgcact
     4141 caaaggcgtt ttgggctaca acaccctgcc cttggtttcc atggacttca accacaccac
     4201 cgaagccagc cacttcgacg caacgctgac caaagttgtt gacggcaaca tggtcaaagt
     4261 gttcgcttgg tatgacaacg aatggggctt cagctgccaa atgctgaaca ccgcacgccg
     4321 tatgttcgga cttgaagtgc gcccgctcaa ataagcaata aaccgtcaaa caaaatgccg
     4381 tctgaaaccc gatgttttca agttccagac ggcatttttc atttttcacc gtgattttac
     4441 ccggctgtcg tcatttctaa tt
//
LOCUS       DAWKQJ010000148         4461 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000148 DAWKQJ010000000
VERSION     DAWKQJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.148"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(46..3078)
                     /locus_tag="WJL20_001709"
     CDS             complement(46..3078)
                     /locus_tag="WJL20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ7683835.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3243..3923
                     /locus_tag="WJL20_001710"
     CDS             3243..3923
                     /locus_tag="WJL20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ7683836.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3952..4311
                     /locus_tag="WJL20_001711"
     CDS             3952..4311
                     /locus_tag="WJL20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ7683837.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALSNIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          912 a         1299 c         1130 g         1120 t
ORIGIN      
        1 tgccgtctga aacagtcttc agacggcatt tccatttgca aaaaatcaag cattgtccaa
       61 ggggacgaga atcgtacggt tgccgttgtg ttccggtgcg gacacaatgc cttccgcctc
      121 catctggtca atcagacgcg cggcgcggtt gtagccgata cgcaaggcgc gctgtacgcc
      181 cgaaatgctg gctttgcgcg ttttcaggac aacggatacg gcctcgtcgt acatcggatc
      241 ggtttcgtcg tcgccgctgc gcccgatgcc gggcagctct tcgctgccgc cgccgctcaa
      301 aatatcgtca acatagtccg gctcgccaaa ctgtttcaaa tattcgacca cgcggtgcac
      361 ctcttcatcc gaggcaaacg cgccgtgaac gcgctgcgga taggcagtac ccggcggcag
      421 gaacagcata tcgccctgac cgagcaggtt ttccgcgccc atttggtcga gaatcgtgcg
      481 gctgtcgatt ttgctggaca cttggaacgc gatacgcgtc gggatgttcg ccttaatcag
      541 acccgtgatg acatcgacac tggggcgttg tgtggcaagg ataagatgga tgcctgccgc
      601 gcgggctttt tgggcgaggc gggcaatcag ttcctcgatt ttcttgcctg ccgtcatcat
      661 caaatcggca aactcatcga ccacgaccac gataaacggc agtttttcca aaggttcggg
      721 atcgtcgggc gtgaggctga acggattgcc gattttttct ccccttgctg cggcttcggc
      781 gattttttga ttgaagcccg caagattacg cacgcccata aagctcatca ggcggtagcg
      841 tttttccatt tcgttaacac accagttcag cgcgtttgcc gccagcttca tatcggtaac
      901 gacaggggcg agcaggtgcg ggatgccttc gtaaatgctc aattccagca ttttcggatc
      961 gatcataatc atacgcacgt cttccggcgc ggctttgaaa agcatagaca gaatcatcgc
     1021 gttgacaccc accgatttgc ccgaaccggt cgtgccggca accagcaaat gcggtgcttt
     1081 gcccaagtcg gttacaacgg gctgtccggt gatgtcctga ccgagcgcga gcgtcagctt
     1141 ggatttggat tcggcaaact cgggcgaatt gaagatttcg ctcaggcgta tcatttggcg
     1201 tttcgggttc ggaagttcca aacccataca ggttttgccg aggatggttt cgacaacgcg
     1261 gatggaagcc acgccgagcg aacgcgccaa gtctttttcc agatttagaa cggaattgcc
     1321 gcgcacgccg acatcgggtt cgatttcata acgcgtaatc acggggccgg aataagaatc
     1381 gacaaccttg accttgactt tgaactccgc caatttttct tcgatggtga tgctgttttc
     1441 caacagttct tcttcggttt gcgtcgcctc gggattgaac agcggcggca gaagcaggtc
     1501 ggttgtcggc aggtgttcgt ataccgcatc ggtttcttca gattggaacg ccccttcatc
     1561 cgcttccgta tccgataccc ggcaggacgg gcgttcagac ggcacatctt caaacggaca
     1621 aatcgcctga ctgtcatgcc cgaacgcttc ggtttccgac agatattgcc cgtttgaccg
     1681 ctctgccgca ccttcactgc cgtcatctgc aatagcggcg gtttcctcct gccaacctcc
     1741 attcaaaaca tcatcggcaa gatggtcggt ttcagcaatg cggctgcgtt gcgcctgctc
     1801 gaatcctgtc ggcggctcat aggtacggtt gtagatttcc gatacgggag gcggcggcgg
     1861 aatatcgatt gcgggcatgg gaactttcgg cacttcgggt ggttcgacca cccaagcatc
     1921 gggcgcagca ggttcttcaa tatggatatc ggcagcttca tcataaaccg gaccgccgta
     1981 tcccacagac gaaacggttt ccgtgaaaac attttcggtt tcagacggca tttcgacatc
     2041 ccgtttcccg acaaccgtcc gactttccgg aatcaggcgc gaaatccgcg cctccgcaga
     2101 aaccgttgcc ttgttcggtt ctttcaaatt gacggaaacg cggcggcagg catcgcggac
     2161 ggctgtgccg tctgaaacgg actgtccttg ggaaacatcc ggggattttg cctccgcctg
     2221 ccctgcatga cggcggaacg gcgcattccg tgcattttcg gctttgactg cagaaaaggc
     2281 agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact cggaaaacgc
     2341 ttctttgtcc gcatcgaaca tacgggaata cgtgcgttga agcacagggt cgtccaaacc
     2401 gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga ccgctgtttc
     2461 gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg cgctgtttaa
     2521 agcacgcgtt gcttcttcca aagtgatttc cttaaaaacc ggacggacgg gcgaaatttc
     2581 gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc ggcggttgtc
     2641 gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag cttctgcttc
     2701 ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg atgcggtcgg
     2761 aacatccgca cgatttgcag aagcaggcgg aacgggcctg tccggttcgg attcgtgcgc
     2821 cagatagttt cggcagaaac ggacgacacc gacaaacagc caatacactg ccgttttgac
     2881 ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt tgatttcagg
     2941 cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc gcatccactc
     3001 gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca aaataacgat
     3061 caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg ggtttatttt
     3121 aacgtgtttc catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac cgattcggct
     3181 ttcggtttgc ccccgccgac agtataatag ccgtcctttc ccttaatctt tgcggcattg
     3241 ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg tggattgtcc
     3301 ttatcctgat actggcagct tccgcgccgt ccgcattccg ctcgcttgcc cgctaccgtt
     3361 ccgcccttcc cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac gcctctgccg
     3421 gcggcggaca gcttgcccag atgaactacc acctgctcgc cgtcaatctg gttgcactga
     3481 tggtgggcac ttccgccgcc ttttggcttg ccgcgctgct gatgctgccc tactgcctgc
     3541 tgtttgccga ttctgccggc gcatatccgc cgaacgcttt ggtgctgatt ctgcccgcgc
     3601 tggtcgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat attttcatct
     3661 ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgttgacc gggctggtgc
     3721 tgaccggtat tttggatgct gcaaacgctt ttccgtccga aatattgtgg acgaccgccc
     3781 tgcccgtctt tattctgctg gcgtgggcgg aagccttcct cagcggcatt tcaactgcca
     3841 tatttgttgc actgaagccc cattggatta acactttcga cgacaaccgc tatcttaagt
     3901 ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac catgctttcc
     3961 aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg gctgctcaac
     4021 ctcgccgttc ccgcatcgtt gtctcccgcc acgggcaacc tgtttgccaa ctggacgggc
     4081 gcgctgctca tcggcatctt tgccgaaacc gtcaaccatc cgcaatggaa gctgctgctg
     4141 attaccggtt ttctcggcag cctgaccacg ctttccggat tttcactgga aaccgtaacc
     4201 ctgctccaat cgaatcgtcc cgcttcagca ctttccaata ttttcctaca cacggcaggt
     4261 tcgctgctgc tgacttggct cgggctgaag ataggggcgg cagtcaaata atcgcaaaaa
     4321 attgcttcct ttggttttga cacaaactat acaagtccta acatcttaat catggcgcag
     4381 catataagcg tggatttgca gcccaatcta tactttctga ttctattcat taagccatta
     4441 tgcttattcc tcaatcacgg t
//
LOCUS       DAWKQJ010000149         4445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000149 DAWKQJ010000000
VERSION     DAWKQJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.149"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..1205)
                     /locus_tag="WJL20_001712"
     CDS             complement(63..1205)
                     /locus_tag="WJL20_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ7683838.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTVSRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAHSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WJL20_001713"
     CDS             complement(1254..1676)
                     /locus_tag="WJL20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683839.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WJL20_001714"
     CDS             complement(1669..2478)
                     /locus_tag="WJL20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7683840.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQAWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WJL20_001715"
     CDS             complement(2560..2937)
                     /locus_tag="WJL20_001715"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ7683841.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WJL20_001716"
     CDS             complement(3137..3904)
                     /locus_tag="WJL20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7683842.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="WJL20_001717"
     CDS             complement(3914..>4445)
                     /locus_tag="WJL20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7683843.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1108 a         1113 c         1073 g         1151 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttcccgatct
      181 ccggtaaaca ttgccgccaa ccagcctgcg taatatttct tcaaccacgt ccccaactgc
      241 ggataaagcg cctgcaaggc gcggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggacgg acggagtgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccaccccggc actgcgtgcg ttggccaatg ccgtctgaat gatgcggcgg
      421 tcgctgtcgc tgcccgaaat cggtgcaaaa gccggtttaa cctgcgcttc ggcacggcgg
      481 cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcg ggcggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcaatccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcga gacggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gatgaagatt tcgacattgc gttcgtccaa aaaggcgtgg
      781 atgcgaacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgtaaag taaaccaatt aaaccagttg
      961 atatttttgg cgagtttgta gatgtcgcgc tcattgcggt atgtcccttt ggtcaggcgc
     1021 agcaggatac ggctgacagt acgcgaatgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccgcggcagg aaacgccgcc gtcaaacact tgaacatcgg tacagccgag gcttccgagt
     1141 tcttgagata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgctccg tgccggcggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacagata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaga cgaccacatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 ttcctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgttct ccccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcaggc agcatggggg caacggtaaa atcacccgcc ttctgccacg accatgcctg
     2161 tccttcgcgc gattgcggtt cgcccgtcca ttggccgggg tttacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc aaggtagttg acgggtttgc ccgcctgagg
     2821 aaattcaagc agctcttccg gagtgaggat acgcacggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaaa ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAWKQJ010000150         4439 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000150 DAWKQJ010000000
VERSION     DAWKQJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.150"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..2022
                     /locus_tag="WJL20_001718"
     CDS             175..2022
                     /locus_tag="WJL20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7683844.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAAMGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADIPI
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGTVPAR"
     gene            2311..2523
                     /locus_tag="WJL20_001719"
     CDS             2311..2523
                     /locus_tag="WJL20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ7683845.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2655..3101
                     /locus_tag="WJL20_001720"
     CDS             2655..3101
                     /locus_tag="WJL20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ7683846.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            3122..3271
                     /locus_tag="WJL20_001721"
     CDS             3122..3271
                     /locus_tag="WJL20_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683847.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3300..3692)
                     /locus_tag="WJL20_001722"
     CDS             complement(3300..3692)
                     /locus_tag="WJL20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ7683848.1"
                     /translation="MTTSTKPYILRALCEWCSDNSLTPHILVRVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            complement(3761..4366)
                     /locus_tag="WJL20_001723"
     CDS             complement(3761..4366)
                     /locus_tag="WJL20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7683849.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIDALTPAEKAMRK"
BASE COUNT         1100 a         1245 c         1211 g          883 t
ORIGIN      
        1 ggcaaaggca gcggttctca ccaaaacggc aaatgattga aaaatataaa ttatgtcaga
       61 aacattccaa agccggtgca gtttcggcat ataattcggg caaacgcctg ttcagacggc
      121 attttgtctt ttccaaccct gaccgttcag ggttccgatt cttaaggaaa tccgatgtac
      181 ctaccctcta tgaagcattc cctgccgctg ctggcggccc tggtgcttgc cgcgtgttct
      241 tcgacacacg cgcccagcag caagaccctg gcagacaata tagaaactgc cggtctttcg
      301 gcaagcgttc ccacccgccc tgccgaaccc gaaggaaaaa cgctggcaga ttacagcagc
      361 tacccgtccg cactggatgc agtgaaacag aaaaacgatg ccgccgtcgc cgcctatttg
      421 gaaaacgccg gcgacagcgc gatggcggaa aatgtccgca acgagtggct gaagtctttg
      481 ggcgcacgca gacagtggac gctgtttgca caggaatatg ccaaactcga accggcaggg
      541 cgcgcccaag aagtcgaatg ctacgccgat tcgagccgca acgactatac gcgtgccgcc
      601 gaactggtca aaaatacggg caaactgcct tcgggctgca ccaaactgtt ggaacaggca
      661 gccgcatccg gcttgttgga cggcaacgac gcctggaggc gcgtgcgcgg actgctggcc
      721 ggccgccaaa ccacagacgc acgcaacctt gccgccgcat tgggcagccc gtttgacggc
      781 ggtacacaag gttcgcgcga atatgccctg ttgaacgtca tcggcaaaga agcacgcaaa
      841 tcgccgaatg ccgccgccct gctgtccgaa atggaaagcg gtttaagcct cgaacaacgc
      901 agtttcgcgt ggggcgtatt ggggcattat cagtcgcaaa gcctcaatgt gcctgccgcc
      961 ttggactatt acggcaaggt tgccgaccgc cgccaactga ccgacgacca aatcgagtgg
     1021 tacgcccgcg ccgccttgcg cgcccgacgt tgggacgagc tggcttccgt aatctcgcat
     1081 atgcccgaaa aactgcaaaa aagcccgacc tggctctact ggctggcacg cagtcgcgcc
     1141 gcaatgggca acacgcaaga ggcggaaaaa ctttacaaac aagcggcagc gacgggcagg
     1201 aatttttatg cggtgctggc gggggaagag ttggggcgca aaatcgatac gcgcaacaat
     1261 gtgcccgatg ccggcaaaaa cagcgtcctc cgtatggcgg aagacggtgc agtcaaacgc
     1321 gcactggtat tgttccaaaa cagccgaacc gccggcgatg cgaaaatgcg tcgtcaggct
     1381 caggcggaat ggcgttttgc cacacgcggc tttgacgaag acaagctgct gaccgccgcg
     1441 caaaccgcgt tcgaccacgg tttttacgat atggcggtca acagcgcgga acgcaccgac
     1501 cgcaaactca actacacctt gcgctatatt tcgccgttta aagacacggt aatccgccac
     1561 gcgcaaaatg ttaatgtcga tccggcttgg gtttatgggc tgattcgtca ggaaagccgc
     1621 ttcgttatag gcgcgcaatc ccgcgtaggc gcgcaggggc tgatgcaggt tatgcctgcc
     1681 accgcgcgcg aaatcgctgg caaaatcggt atggatgccg cccagctcta caccgccgac
     1741 ggcaatatcc gtatggggac gtggtatatg gcggacacca aacgccgcct gcaaaacaac
     1801 gaagtcctcg ccaccgcagg ctataacgcc ggtccaggca gggcgcgccg atggcaggcg
     1861 gatatcccca ttgagggcgc ggtatatgcc gaaaccatcc cgttttccga aacgcgagac
     1921 tatgtcaaaa aagtgatgac caatgccgcc tactacgccg ccctcttcgg cgcgccgcac
     1981 atcccgctca aacagcgtat gggcaccgtt cccgcccgct gacgtaccga tgccgtctga
     2041 aacccgcccg gtcttttaga cggcattttt atcccgaacg gcattgacgg cgaaccataa
     2101 atataagaca atccgaaaat tgtttttcct gctttttcaa gcagcttgac acggcacaag
     2161 ccgacccgtt aggaggtgat gtttccgtca cggcgcgtat cccgccgccg aaaggcacag
     2221 cgatacggaa aacctacaac accgtctgcc ctaccctttc caccgatatg atgggcagat
     2281 gcaacaacag aatttattaa aggaaataaa atgcctgcaa tccgcgtaaa agaaaacgaa
     2341 ccctttgaag tcgctatgcg ccgtttcaaa cgcgccgtag aaaaaaccgg cctgctgacc
     2401 gagctgcgcg cccgcgaagc ctacgaaaaa ccgactaccg aacgcaaacg caaaaaagcg
     2461 gcagccgtaa aacgcctgca aaaacgcctg cgcagccaac agctgccgcc caaaatgtac
     2521 taaacgttca agtacagatt acaggtcagc cctgtgatat gaggacacac cgcaagacct
     2581 gctctgcggt gtgttttgct tttcagacgg catcgaaacc cgccgtctcc atccgacatc
     2641 ccagcgagga cattatgagc ctgaaaatcc gccttaccga agacatgaaa accgcgatgc
     2701 gcgccaaaga ccaagtttcc ctcggcacca tccgcctcat caacgccgcc gtcaaacagt
     2761 ttgaagtgga cgaacgcacc gaagccgacg atgccaaaat caccgccatc ctgaccaaaa
     2821 tggtcaaaca gcgcaaagac agcgcgaaaa tctacactga agccggccgt caggatttgg
     2881 cagacaaaga aaacgccgaa atcgaggtac tgcaccgcta cctgccgcaa atgctctccg
     2941 ccggcgaaat ccgtactgcg gtcgatgccg ccgttgccga aaccggcgcg gcaggtatgg
     3001 cggatatggg caaagtgatg ggcgtattga aaacccgcct cgccggcaaa gccgatatgg
     3061 gcgaagtcaa caaaattctg aaagccgtac tgaccgcctg attgtatgaa tataaggaaa
     3121 aatgccgtct gaagcccgta tcgcaggttc agacggcatt ttcaatgtcc caatatcgaa
     3181 tcggcagggg caacacggtt tcgatacacc gaaacgggtt ttgccgataa acggattccg
     3241 tttgcgcccc atcggacaaa atgccgtctg aaacacgatt ccgttcagac ggcatagatt
     3301 tatttgacca atttcaagcc ttttttggcg ggtcggggcg cggtttcggc agaggtgttt
     3361 tcagacggag tgtcggggcg gtactcctcc aattcaaacc ccataccttc tccggtctcc
     3421 cgtgcgaaaa ggctgaggac gtgtccgaca ggtatccata tgtcgtgcgc ctgtccgccg
     3481 aagcgggcgg aaaaactgat ccaatcgttg tcgatttgaa ggttttgcgt ggcggtcgcg
     3541 ccgatgttga gcataatttc gttgtcgcgg acatactgca taggaacgcg cgtgtgttcg
     3601 ttgacccgga caaggatgtg cggtgtgagg ctgttgtcgc tacaccattc gcagagggcg
     3661 cggaggatgt agggtttggt ggaagtagtc ataatgctcc ccataatgtg cctgaatata
     3721 gaaatgcctg aaaccgtcag gttttacagg catttcaaac ttatttgcgc atcgcctttt
     3781 ccgcaggtgt cagcgcgtcg ataaaggctt cgcgttggaa gatgcgctcg gcgtatttga
     3841 gcagcggcgc ggcacttttg cccagtttga catcgtagtg gtcaagccgc cacagcagcg
     3901 gggccagggc gacatcaatc atagaaaaat cttcgccgag aatgtatttg cttttgctga
     3961 acgaaggggc gagcatggtc agaccgttgc cgatggcttc gcgcgctttt gcctgttcct
     4021 tgttagtggc ggcggggttt tccaacactt ggacatggtt gaacaattct ttttccatac
     4081 ggtacagcac cagccggccc ctgccgcgca taacgggatc gccgggcata agctgcggat
     4141 gggggaagcg ttcgtcaatg tattcgttga tgatgttgga ctcgtgcagt accaaatcgc
     4201 gctcgaccag cacagggact tggttgtacg gattcatgac ggcgaggtct tcgggtttgt
     4261 tgtaaatatc gacgtctttg atttcaaaat ccataccttt ttcgtacaaa acgaagcggc
     4321 agcggtggct gaaggggcag gtaatgccgg aatagagggt catcataata attgtcgctc
     4381 ctgtgtgatg cctgcaaaac ggctgattta tagtggatta acaaaaacca gtacggcgt
//
LOCUS       DAWKQJ010000151         4418 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000151 DAWKQJ010000000
VERSION     DAWKQJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.151"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            38..461
                     /locus_tag="WJL20_001724"
                     /pseudo
     CDS             38..461
                     /locus_tag="WJL20_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WJL20_001725"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WJL20_001725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ7683850.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WJL20_001726"
     tRNA            1210..1285
                     /locus_tag="WJL20_001726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WJL20_001727"
     tRNA            1311..1386
                     /locus_tag="WJL20_001727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WJL20_001728"
     tRNA            1410..1485
                     /locus_tag="WJL20_001728"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WJL20_001729"
     tRNA            1507..1582
                     /locus_tag="WJL20_001729"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="WJL20_001730"
     tRNA            1608..1681
                     /locus_tag="WJL20_001730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="WJL20_001731"
     tRNA            1784..1873
                     /locus_tag="WJL20_001731"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="WJL20_001732"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="WJL20_001732"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ7683851.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3943)
                     /locus_tag="WJL20_001733"
     CDS             complement(3080..3943)
                     /locus_tag="WJL20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ7683852.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDRRQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(3956..4387)
                     /locus_tag="WJL20_001734"
     CDS             complement(3956..4387)
                     /locus_tag="WJL20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ7683853.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1091 a         1052 c         1154 g         1121 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgccctcaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tacggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaatg ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatcttcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgtttcgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggtc cagctgccgc cggtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gccgctgccg ctgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca catcaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac gcctgccccg caatcacggc attaatcgcc cagccgccaa
     3841 acaccggata caccagcatc aaaaggtttt ccagtccgac cagaaaaaat gtgccaatca
     3901 atcgcttgcg gtggttttgg ctatgtgttt caacattttc cacatatttg cccccctatt
     3961 ttttattttc cgagattgtt ttttccatca cttcagccag tgcatccaaa tcggcaaaca
     4021 gccgagttgt gcgcttgtcg ccgaatgtgg caaatacttt gtcgctgaat tcctgcgcgc
     4081 tttctgttaa aggtgcggca taggctttgc ctgtttcggt caacgacagc aaccgtttgc
     4141 gccggtcctg ttcgccttcc tgccattcaa tcaacccttg tccggcaagg gttttgcata
     4201 cgcctgaaac ggtctgtttg ggcaggctcc acttttcgcc gatatgcttt tgcgtgcggc
     4261 tgccttcggt tgccagggta taaagtacgg caaagaggtt gtaattcaga tcctgctgcc
     4321 cgatccattt gtcgaagaca ttgcaaatca ggttgatacg ggtgccaagt tggtcgagtt
     4381 ggttcataat tggtcttgat attgactaaa tttgtgcg
//
LOCUS       DAWKQJ010000152         4386 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000152 DAWKQJ010000000
VERSION     DAWKQJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.152"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(229..2157)
                     /gene="dnaK"
                     /locus_tag="WJL20_001735"
     CDS             complement(229..2157)
                     /gene="dnaK"
                     /locus_tag="WJL20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ7683854.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2365..2637)
                     /locus_tag="WJL20_001736"
     CDS             complement(2365..2637)
                     /locus_tag="WJL20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683855.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2823..3509
                     /locus_tag="WJL20_001737"
     CDS             2823..3509
                     /locus_tag="WJL20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7683856.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3641..3979
                     /gene="erpA"
                     /locus_tag="WJL20_001738"
     CDS             3641..3979
                     /gene="erpA"
                     /locus_tag="WJL20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ7683857.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3996..4142
                     /locus_tag="WJL20_001739"
     CDS             3996..4142
                     /locus_tag="WJL20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683858.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4139..>4386
                     /locus_tag="WJL20_001740"
     CDS             4139..>4386
                     /locus_tag="WJL20_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683859.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
BASE COUNT         1007 a          882 c         1157 g         1340 t
ORIGIN      
        1 tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggtttaa
       61 atttaatcca ctatataaaa aatttccaga gaaccgatac aacagttgga acttgggttt
      121 gggaatatta cggtagatga acttggaacc tctgttatgc tatggtcttt tatctcaatt
      181 gaaaaaagcg cgaatcaaac ggttcgcgct tttttcagac ggtattaatt attttttgtc
      241 gtcttttact tcttcaaagt cggcatctac gacatcatcg tctttctttg cagaagcatt
      301 ggcttgttcg ctttcgcctg cttgggcttc agcttgtgct tgagcgtaaa ccatttcccc
      361 cagtttttgg ctggctgcgc ccagcgcctc ggttttggca tcgatagcgg ctttgtcgtc
      421 gcctttaact gcttcttcgg cttctttcag cgcggcttcg attttttctt tctcggctgc
      481 gtcgagtttg tcgccgtagt cggccaaaga ttttttcaca gagtgaatca gggcttcggc
      541 ttggttgcgg gaagcgacca attcagtcag ttttttatct tcctcggcat tggcttcggc
      601 atctttcacc atgcgttcga tttcttcttc gctcaaacct gaagaacctt ggatggtgat
      661 gttggctgct ttgccagtgc ctttgtcttt ggcggaaacg tgcaggatac cgttggcgtc
      721 aatatcgaag gtgacttcga tttgcggcat accgcgcggt gcgggtgcga tgtcgcccaa
      781 gttgaactga cccaaagatt tgttggcaga agcgcgttcg cgttcgcctt gcagtacgtg
      841 gatggttact gcgctttggt tgtcttcggc ggtagagaac acttgcgacg ctttggtcgg
      901 gatggtggtg ttcttttgaa tcagcttggt catcacgccg cccatggttt cgatacccaa
      961 ggacagaggg gttacgtcca gcagcaatac gtcgctgcgg ccgccgctta acacttcgcc
     1021 ttgaatcgct gcgcctacgg cgacagcttc gtcagggttc acgtctttgc gcggctcttt
     1081 gccgaagaag tctttaacgg cttcttgtac tttcggcata cggctttgac caccgaccaa
     1141 aatcacgtcg tcgatgtcgc cagtgctcag accggcatct ttcaatgcgg tgcggcaagg
     1201 ctcgatagag cgggcaatca ggtcttcaac caggctttcg aatttggcgc gggtaatttt
     1261 catcgccaag tgtttcgggc cggttgcgtc catggtgatg tacggcaggt tgatttcggt
     1321 ttgctggccg ctggacaatt cgattttggc tttttcggca gcttctttca gacgttgcag
     1381 agccatgacg tcttgtttca aatcaatgcc ttgttctttt ttaaactcgt caatgatgta
     1441 gtcgatcaag cgttggtcga agtcttcacc gcccaagaaa gtatcgccgt tggtagccaa
     1501 tacttcaaat tgtttgtcgc cgtcgaggtt ggcgatttcg atgatggaaa tatcgaaagt
     1561 accgccgccc aagtcatata cggctacttt gcggtctttg ttgtcgcctt tgtccatacc
     1621 gaatgccaaa gcggctgcgg tcggctcgtt gatgatgcgt ttcacgtcca aaccggcgat
     1681 acggcctgcg tctttggtag cttgacgttg gctgtcgttg aagtaggcag ggacggtaat
     1741 cacggcttcg gttacttttt cgcccaagta agcttcggcg gcttctttca ttttacgcag
     1801 gacttctgcg gaaatttgag gaggagacag ctctttgcct tgtgctttta cccatgcgtc
     1861 gccgttgttg gctttgatga tttcgaaagg catagattcg atgtcgcgtt ggacttcttt
     1921 gtcttcaaat ttgtggccga tcaggcgttt ggcggcgtaa atggtgtttt tagcgttggt
     1981 tacggcttgg cgtttggcag gcgcaccgac gaggatttcg ccgccgtcca aataagcgat
     2041 aacggacggc gtggtgcgtg cgccttctgc gttttcgatc actttggttt gaccgttttc
     2101 ggaaatggcc aaacaagagt tggttgtacc taagtcgata ccgattactt ttgccatgtg
     2161 gataatccta tttgattttg cttattttga gaaatatgtt ggaacatttt gtcccgatgg
     2221 gctgtaaata gggcgggcgg cgggctgttt caagctacag catggctata agtatataac
     2281 tttatgaata tattggtttt atatttgatt taatacattt ggctccaatg catttaagca
     2341 taatatttca aatggcaggc aggtttattc atagacgatg ccggcgagca tttcctgttc
     2401 gttcaagttg ccgtactctt tttcccagtc gtgtgaagac tcgatgatgt cgcattcttt
     2461 ggaaagggag acttgttctg catccatatc tttggcgttc agtatgttga attgttcgca
     2521 cagggatgcg gataaagtga tgtcaggctg tttggcttca gaacggtttt cttggaaggc
     2581 aaagcagaat gcggtaaatg ccgcagcata gataagatat ttgccggttt tcttcatttt
     2641 ctatcctttt tctgtcaact caggattaaa cctatggaaa atcttaaaaa ttatgtattg
     2701 agtaagaaaa atcataattt aaatttagtt tatcatagtt gttccgtttt tgggatagct
     2761 aaggtaaaat atatttcatg tttactttag atgattgaat gaaggggagc ggaaggatat
     2821 ttatggaaac ctttaaagac agactggttt ttttatggaa aagcgaagcg aagcaggcaa
     2881 aaatcgcatc cgatattgaa atgacgattg cgggcttcag caggatatgg aatgaaggcg
     2941 gtctgccgaa gtctgaaaca ttaaaaaaaa tcaagcagtt gaaggggtgt agtatcgatt
     3001 ggctgctgac cggggagggt aatccgtttc cggatgaagc cccaaaaaaa tcccttgctt
     3061 acgatacctt gggaaatgaa gtcgatacgg acgagtttgt ctttgtgccg agatatgata
     3121 ttcgggcggc tgcgggatac gggcagtttg tcgatcatga ggaaccggta tttacaatgg
     3181 cgttcagacg gcattggatt gagaattatg ttacccgcga tacgaaaaac ctgtctgtaa
     3241 tttccgtcaa gggggattcg atggaggggg ttttgaatga cggcgattcg attttggtca
     3301 atcatggtga aaatacgccg agggacggtc tgtatgtgtt gcggattaat gaaaatctgc
     3361 tggttaaacg tttacagatt gtaccgggcg ggattatcaa tgtgatttct gcaaacgagg
     3421 cttatcctgc ttttgaaatc aatttgaacg atttgaccga tgatgtggag attatcgggc
     3481 gtgtcgagtg gttcggcagg acgatttgag tttggggctt gaaattgcag gcggtcaaac
     3541 ttatctattg gaacaattcc tttttcaaag gcgaagcctg cttgccttta aagggggttt
     3601 gagagagaat gcagaaaata ttatattaag gaataacacc atgtcggatg aaagccctat
     3661 tatttttact gacagctgct gtgccaaagt tgccgatttg attgccgaag aaaacaatcc
     3721 cgatttgaaa ttgcgggttt ttgtcaatgg cggcggctgt tcgggtttcc agtacggatt
     3781 tacttttgac gaaatcaaaa acgacgacga ttttgaaatt gagaaaaacg gtttggtctt
     3841 tttggtcgat ccgatgagct atcaatatct ggtcggtgcg gaaatcgact atacggaaag
     3901 tttgcagggt tcgcaattcg tcatccgcaa tccgaatgcg gaaacaacct gcggttgcgg
     3961 atcgtcgttt tccgtatgac cgcttggttt gtgtgatgcc gtctgaacgt tcagacggca
     4021 tttttacttt tggaaaatat attatcggga tgaattcaca tataatccga ttgtttgaag
     4081 atgaatcggg tttcccgaaa ggaacgggcg gaacggtatc aggcgtattt gttcccttat
     4141 gattgagatg agtaaagatt accgaaatga tttgtacgat gtatatgttt cctacccgcc
     4201 ccaagtggat cgcgggctta tccgggagtg cctcagggag aatctcggcg aggaaaaggc
     4261 ggaaggattg atcgaatcgc tcgattccaa acctcaagtg ctggttgagg aaaaatgcac
     4321 ttgggcgaaa cgggaagagt tgcacgatta tttcagctat ttgggtttgg atattattac
     4381 ccggag
//
LOCUS       DAWKQJ010000153         4360 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000153 DAWKQJ010000000
VERSION     DAWKQJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.153"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            266..1186
                     /gene="ribF"
                     /locus_tag="WJL20_001741"
     CDS             266..1186
                     /gene="ribF"
                     /locus_tag="WJL20_001741"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ7683860.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            1327..4116
                     /gene="ileS"
                     /locus_tag="WJL20_001742"
     CDS             1327..4116
                     /gene="ileS"
                     /locus_tag="WJL20_001742"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ7683861.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVACQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLASIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVADMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1064 a         1201 c         1168 g          927 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gggttgcttg tctcggttta cctggttaaa aaagaacgat
       61 tttcactgat gttgcatcag gtttggggcg atgtttcaac acatagcacc gcgcctgctg
      121 cgcgctttat gcgtttggcg cgttcggctg cgggaaattt gcctactttt cccgcgtcgg
      181 gcgggcgtaa cgggcggcac actgtctata aaccgcaata ccgtttacaa tgaccgcctg
      241 tttcaccaca tacccgaaca taacaatgag aatcaggctg gggcggcaca acgcgcccga
      301 ctttccgcac ggggccgccg taaccatagg caattttgac ggcgtacacc tcggacacaa
      361 acacatcctc caaaaactcc gcctcgaagc cgatacgcgc ggattacccg tcgtgaccgt
      421 cgttttcgaa ccccaaccca aagaattttt cgcactccgc accggcagga tgccaccgtg
      481 tcggatcagc cccctgcgca ccaagctcga attattggaa ggcacaggct gtgtcgatgc
      541 cgtctgggtt ttgcgtttcg atcaaaattt ttccgaaata tccgcgcaag ggtttatcga
      601 ccgcctgctg cgtcaaacct tgaatacgcg ttatttgctc gtcggcgacg atttccgttt
      661 cggtgcgggg cgggaaggct gttttgaact tttggcacaa cagcccgata tgcagaccga
      721 gcgtacgcct tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg tgcgacaagc
      781 cctttcagac ggcaaccttg cctatgcgaa aaaacttttg ggacacgact acgtcttgag
      841 cggcagggtg gtgcacggca gaaaactcgg acgcacctta aacgccccga ctgccaacat
      901 ccgcctgccc cgccaccgtt atgcactcgg cggcgtgttc gtcgtcgaag cggacggcgc
      961 gttcggcacg cggcgcggcg tggcgagctt cggcttcaat cccaccgttg atagcgactg
     1021 ttttcaaaag ctggaagtcc acctgttcga ctttcaaggc gatttgtacg gacaacggtt
     1081 gaacgtccgc ttcctgcaca aactgcgcga tgaggaaaag tttgacggta tggaagaact
     1141 gaaaaggcag attgaagccg atatggaagc cgcaaagtgt tggtagaaaa accttataca
     1201 aaccatccga ttgggctaca atcaaccttt taactgttca gacggcatag gggtttcccg
     1261 ttgtgaaata ctgtttgagg cgcaatgccg tctgaaccga aatattgtaa caatagagat
     1321 taaaaaatga ccgattacag caaaaccgta aacctgctcg aaagcccgtt tccgatgcgc
     1381 ggcaatcttg ccaagcgcga gcctgcatgg ctgaaaagct ggtacgagca aaaacgctac
     1441 caaaaactgc gcgaaatcgc caaaggtcgt ccgaagttca tcctgcacga cggcccgccg
     1501 tatgccaacg gcgacatcca catcggtcac gccgtcaaca aaattctcaa agacatcatt
     1561 atccgcagca aaacccaagc cggttttgac gcgccttatg tgccgggttg ggactgtcac
     1621 ggtttgccta tcgaagtgat ggtagaaaag ctgcacggca aagacatgcc caaagcgcgt
     1681 ttccgcgaat tgtgccgcga atatgccgcc gaacaagttg cctgccagaa aaaagacttc
     1741 atccgtctgg gcgtgttggg cgactgggac aagccctacc tgaccatgga ttttaaaacc
     1801 gaagccgata ccgtgcgtat gctcggcgaa atctacaaat ccggctatct ctaccgcggc
     1861 gcgaaaccgg ttcagttctg cttggactgc ggctcttcat tggccgaagc cgaagtggaa
     1921 tacaaagaca aagtatcgcc tgcgattgac gtgggctatc cgtttaaaga caccgctgcg
     1981 cttgccgctg cattcggttt ggcaagtatc gaaggcaaag cgtttgccgt catctggacg
     2041 actacgcctt ggacgctgcc tgcgagccaa gccgtatctg ccggcgcgga cgtggtgtat
     2101 caactgattg atacgcccaa aggcaaattg gtattggcga aagatttggc ggaagacgcg
     2161 ctcaaacgtt acggcttttc agacggcatc gccatccttg ccgaaaccac cggcgacaag
     2221 ctggaaaacc tgcacatgaa ccatccgttc ctcgaacgcg atattcccat gcttaacggc
     2281 gaacacgtta ccaccgatgc cggtaccggc ttggtgcata ctgcgcccgc gcacggtttg
     2341 gaagactacg ccgtctgcaa caaatacggc atcgagcttt acaatcctgt caacgccgaa
     2401 ggcaaataca tcggcgaaac gccgcgcgtt gccgatatgc gcgtttggga ggcgaacccc
     2461 gtcatcctgc aatggttgga agaaaccggc aaccttttgg caagcagtaa aatcgaacac
     2521 agctacgccc actgctggcg gcacaaaacg ccgctgattt accgcgcgac aggtcaatgg
     2581 tttgtcggta tggacaaagc cggtgccgac ggcaaaaccc tgcgcgacaa agccatcaag
     2641 gccgtggacg ataccgaatt cttcccgtct tggggtcgcg cgcgtttgga agccatgatt
     2701 gaaggtcgtc ctgactgggt ggtttcacgc caacgctatt ggggcacgcc gatgactttc
     2761 tttgttcaca aagaaacggg cgagctgcat ccgaactctg ccgaactttt ggaaaaagtc
     2821 gcgcaacgca ttgaagaaaa aggcatcgag gcttggttct ccctcgataa aagcgaatta
     2881 ttaagcgcgg aagattgcga acattacgac aaactccccg atacgatgga cgtatggttc
     2941 gattccggtt cgacccatta ttccgttgtg aaacaacgcg aagaattgga atggccggcg
     3001 gatttgtatc tcgaaggcag cgaccagcac cggggctggt ttcaatcgtc catgctgacc
     3061 ggctgcgcct catcaatggg acgcgcaccg tataaacagc tgctgaccca cggttttgtt
     3121 gtagacggcg aaggcaaaaa aatgtcgaaa tccatcggca acgtcgtcgc gccgcaagaa
     3181 gtctataacg aattcggcgc ggacatcctg cgtctgtggg cggcatctac cgattacagc
     3241 ggcgaattgg cgatttctaa agaaatcctt aagcgcgtaa ccgaaagcta ccgccgtatc
     3301 cgcaatacct tgagcttttt gtttgccaac ttgagcgact ttaatccgat tgaagatgcc
     3361 gtgcaacagg cagacatggt ggaaatcgac cgctacgccg tggtattggc acgtcagctg
     3421 caagagcgtc tggcaggcga ttactatccg cgctatgctt tccactttgc cgtaaaagac
     3481 attgtttctt tctgctcgga agacttgggc gcgttctacc tcgacatcct gaaagaccgc
     3541 ctctacacca ccaaagccga cagccgcgcc cgccgcagcg cgcaaaccgc cctgtaccac
     3601 atcacgcgca gcctggttct cttgattgca ccgattttgt gctttaccgg cgaagaagcg
     3661 tgggacatca tcggcggcgg cgaagaagac agcgtcctct tccatacttg gcacgagttc
     3721 ccgaccatca acgaaaaaac cgaagccgaa ctggtgaaaa aatggacggc aatccgcgaa
     3781 gcccgcgaag cggtaaccgc cgccatcgag cctttgcgcg ccgacaaaac cgtcggttcg
     3841 tccttgcaag ccgaagccga aattaccgcg ccggaagaaa tggccggcta tctgaatgct
     3901 ttgggcgaag aattgcgctt tgctttgctg gtgtctaaag cagaagtgaa agtaggcagc
     3961 gaacttgccg ttgccgctaa agccagtgat ggtgaaaaat gcgaacgctg ctggcactac
     4021 acccgcgatg tgggcgcggt tgcaggctat gaaaccgtct gcaaacgctg tgcagagaat
     4081 gtcggcggag aaggcgaaac gcgccattac gcctgataaa gtttgagcaa atgccgtctg
     4141 aaaccgccga acagcatttc agacggcatt ttttgtgccg cgatttgtct ttataacggc
     4201 ggaggggttt caagattgcg gtgttgtcgg aatgcaactg aaccgtcatt cccacgaaag
     4261 tgggaatcta gaatctcgag gtttcagtca tttccgatag attcccgccg cgtcaggggt
     4321 ctggattccc gcctgtgcgg gaatgacgga tttcgagatt
//
LOCUS       DAWKQJ010000154         4360 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000154 DAWKQJ010000000
VERSION     DAWKQJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.154"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(161..406)
                     /locus_tag="WJL20_001743"
     CDS             complement(161..406)
                     /locus_tag="WJL20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683862.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(407..703)
                     /locus_tag="WJL20_001744"
     CDS             complement(407..703)
                     /locus_tag="WJL20_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683863.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(694..1047)
                     /locus_tag="WJL20_001745"
     CDS             complement(694..1047)
                     /locus_tag="WJL20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683864.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1283..2710
                     /locus_tag="WJL20_001746"
     CDS             1283..2710
                     /locus_tag="WJL20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ7683865.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2779..4182
                     /locus_tag="WJL20_001747"
     CDS             2779..4182
                     /locus_tag="WJL20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ7683866.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
BASE COUNT         1108 a         1073 c         1144 g         1035 t
ORIGIN      
        1 tttgaagcgg ttgccggctt cctgccgtcc gctaacgcgt ccgtcatcac gccgacaacc
       61 gcctttgtca tcccttgctt atcttccatg gtgcgaatcc tcaaaaacag gcaaaaaaaa
      121 agccctgtta cttgtagaaa gtaaaggacg ttaatttttg ttaatcgtcc cttcttaggg
      181 acgcaatata taagggacgc aatatataag gttttaaaac gctaagaaca aaatccgata
      241 acggctctga agaagtaagt ctttctttat tgcacaagat agcaaacttc cacggcattc
      301 tcgccccacc cgtgccgtcc aagccttttt caaagacata gatggttttc ttggcaatca
      361 cttcgtcagt ttggcggtcg ataatccgta tggtcgcacc tgccactcag tgcctgcgca
      421 gcttggaatc gacattgttt tcgaacgtta ccgcataacg ggcgaggtgt atatgattaa
      481 gtatttgatt tatcggaaaa tctttacctg aataccgaat aatatcggaa tggttgggtt
      541 gcaggacatc cacatagcgg tattccccat cgctaaaaaa tcctaggaaa cgagcaataa
      601 aatttacgcc ttcgctggtc tgtaaagccg cattgtccca catcgggtct ctggtttttg
      661 catctgccga aacggtacgc tcaggtacta ccttcaacag cataatccct tccacattgt
      721 ccgccgtctg gtaaatcttt cccccgccgt tttgcattgt tcgttaaaca cggcttcggc
      781 ttctttgtat tttctgtccc actcttcttg tgcctgtatt tcttctttga tcgggccgaa
      841 ttgtttggga ataatccaaa caaacagcat caggatagcg gcggcggtca ggctgcctga
      901 aaggattttg ccggggttcc gtttgggctt tttataggca aagcggacga gaaaccaaag
      961 caacagcagc atggtgcccc aatagccgat tgagaatagg atggccaaac cttctaggaa
     1021 atggcgtaaa tcgtttgtgg taaacatggg ttgttcctgt ggttaaatgt gcaggctgct
     1081 ttttgccgaa tcttgccgca tctcaaaagc agcctgcgct tcagcgttgc gttacgcagt
     1141 aaaataatga atatttgtaa cggcttgggt attttttgtc aatattcccg cctttccctt
     1201 aacagctgcc gcgctttccg ttaaaattcc tttacatatt tatattgttt cctgtttcta
     1261 tattgccaag gttatactcg ttatgttttt ctccgccctg aaatcctttc tttcccgcta
     1321 cattaccgta tggcgcaatg tttgggcggt gcgcgaccag ttggaaccgc ccaaacgcac
     1381 ggcggaagaa caggcgtttt tgcccgcgca tttggaactg accgatacgc cggtctctgc
     1441 cgctccgaaa tgggcggcgc gttttattat ggcgtttgcg cttttggctt tgttgtggtc
     1501 ctggttcggc aaaatcgata ttgtggcggc ggcttcgggc aaaacggtgt cgggcgggcg
     1561 cagcaaaacc atccagccgc tggaaacggc ggtggttaag gcggtacatg tgcgcgacgg
     1621 gcagcatgtg aaacagggag aaacgctggc ggaactggag gctgtgggaa cagacagcga
     1681 tgtggtgcag tcggagcagg ctttgcaggc tgcccaattg tccaaactgc gttatgaagc
     1741 ggtattggcg gcattggaaa gccgtaccgt gccgcatatc gatatggcgc aagcacggtc
     1801 tttaggtctc tccgatgccg atgtgcaatc ggcgcaggtg ttggcgcagc accagtatca
     1861 ggcatgggcg gcgcaggatg cgcaattgca gtcggctttg cgcggccatc aggcggaatt
     1921 gcagtcggcc aaggcgcagg agcagaagct ggtttcggtg ggggcgatcg agcagcagaa
     1981 aacagcagac taccgccgtt tgcgggccga caattttatt tcggaacatg cgtttttgga
     2041 gcagcagagc aaatcggtca gcaattggaa cgatttggaa agtacgcgcg gtcagatgag
     2101 gcagattcag gcggccattg cacaggcgga gcagaatcgg gtgctgaata cgcagaacct
     2161 gaaacgcgat acgctggatg cgctgcgcca ggcaaacgaa cagattgacc aataccgcgg
     2221 ccaaacggat aaggcaaagc agcggcagca gctgatgaca atacagtcgc ctgcggacgg
     2281 cacggtgcag gaattggcca cctatacggt gggcggtgtg gtgcaggctg cccaaaaaat
     2341 gatggtgatt gcgcccgatg acgacaaaat ggacgtggaa gttttggtat tgaacaaaga
     2401 catcggtttt gtggaacagg gacaggatgc ggtggtgaag attgagagtt ttccctatac
     2461 gcgctacggt tatctgacgg gcaaggtgaa aagtgtcagc catgatgcgg taagccacga
     2521 acagttgggc ttggtttata cggcggtggt gtcgctggac aaacatacct tgaatattga
     2581 cggcaaagca gtgaatctga cggcgggcat gaatgtcacg gcggagatta aaacgggtaa
     2641 acggcgggtg ctggattatc tgttaagccc gctgcaaacc aaattggacg aaagctttag
     2701 ggagcgatag gcggatccgt actgggcatt tgttatccgc cggttcggac atgcagactg
     2761 cctgaaacca ttgcccggat gacattgctc aatctaatga taatgcaaga ttacggtatt
     2821 tccgtttgcc tgacactgac gccctatttg caacatgaac tattttcggc tatgaaatcc
     2881 tatttttcca aatatatcct acccgtttca ctttttacct tgccactatc cctttcccca
     2941 tccgtttcgg cttttacgct gcctgaagca tggcgggcgg cgcagcaaca ttcggctgat
     3001 tttcaagcgt cccattacca gcgtgatgca gtgcgcgcac ggcaacaaca agccaaggcc
     3061 gcattccttc cccatgtatc cgccaatgcc agctaccagc gccagccgcc atcgatttct
     3121 tccacccgcg aaacacaggg atggagcgtg caggtgggac aaaccttatt tgacgctgcc
     3181 aaatttgcac aataccgcca aagcaggttc gatacgcagg ctgcagaaca gcgtttcgat
     3241 gcggcacgcg aagaattgct gttgaaagtt gccgaaagtt atttcaacgt tttactcagc
     3301 cgagacaccg ttgccgccca tgcggcggaa aaagaggctt atgcccagca ggtaaggcag
     3361 gcgcaggctt tattcaataa aggtgctgcc accgcgctgg atattcacga agccaaagcc
     3421 ggttacgaca atgccctggc ccaagaaatc gccgtattgg ctgagaaaca aacctatgaa
     3481 aaccagttga acgactacac cgacctggat agcaaacaaa tcgaggccat agataccgcc
     3541 aacctgttgg cacgctatct gcccaagctg gaacgttaca gtctggatga atggcagcgc
     3601 attgccttat ccaacaatca tgaataccgg atgcagcagc ttgccctgca aagcagcgga
     3661 caggcgcttc gggcagcaca gaacagccgc tatcccaccg tttctgccca tgtcggctat
     3721 cagaataacc tctacacttc atctgcgcag aataatgact accactatcg gggcaaaggg
     3781 atgagcgtcg gcgtacagtt gaatttgccg ctttataccg gcggagaatt gtcgggcaaa
     3841 atccatgaag ccgaagcgca atacggggcc gccgaagcac agctgaccgc aaccgagcgg
     3901 cacatcaaac tcgccgtacg ccaggcttat accgaaagcg gtgcggcgcg ttaccaaatc
     3961 atggcgcaag aacgggtttt ggaaagcagc cgtttgaaac tgaaatcgac cgaaaccggc
     4021 caacaatacg gcatccgcaa ccggctggaa gtaatacggg cgcggcagga agtcgcccaa
     4081 gcagaacaga aactggctca agcacggtat aaattcatgc tggcttattt gcgcttggtg
     4141 aaagagagcg ggttagggtt ggaaacggta tttgcggaat aaagcaggct gaaacggtta
     4201 tgaaattccc aaagcagcct gcaccccgtt tcgaaagtgc aggctgcttt gggattgatc
     4261 cgatactttc acattctcat tgcctttaaa taaagcacag ttaaaaacac atttaaatta
     4321 agtgcgaatt aattcaaatg tatattcaat taaaatcaaa
//
LOCUS       DAWKQJ010000155         4276 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000155 DAWKQJ010000000
VERSION     DAWKQJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.155"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            539..2266
                     /gene="ilvB"
                     /locus_tag="WJL20_001748"
     CDS             539..2266
                     /gene="ilvB"
                     /locus_tag="WJL20_001748"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ7683867.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDSLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2277..2768
                     /gene="ilvN"
                     /locus_tag="WJL20_001749"
     CDS             2277..2768
                     /gene="ilvN"
                     /locus_tag="WJL20_001749"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ7683868.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2835..3128
                     /locus_tag="WJL20_001750"
     CDS             2835..3128
                     /locus_tag="WJL20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ7683869.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3223..4236
                     /gene="ilvC"
                     /locus_tag="WJL20_001751"
     CDS             3223..4236
                     /gene="ilvC"
                     /locus_tag="WJL20_001751"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ7683870.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1117 a         1022 c         1072 g         1065 t
ORIGIN      
        1 aatcaatata ttaagtcgtt gttagccctc ttggttctgt caatgccgtc caatacagtc
       61 cggtgcaatg agccattttc aggaagtatg gttgggaata tttttgatat atcaaaaata
      121 aaatcaagtt caatcaatat cccgagtctt tattgggatg aaaccctgta aattttacaa
      181 ttcttgatat cgaagaaggg tttttcacat ctgcccctcc tacacaaact gtcaacaatt
      241 tccatctttc aaaaaaggta ctcctgattt tattctttta aaatcaatca ataatttctc
      301 tgctttcaaa aaatattaaa agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt
      361 atcgtttcta ctctttttca caaactctgt gttcctttcc aattgattgg ataatccgtc
      421 tgttgccgtg ttcccccaat gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca
      481 ggtgctgtgg caaagcggtt tgtccctatt ggtttgaaac cgtataaaag aggtcgttat
      541 gcagttatca ggcgcgcaaa tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt
      601 tttcggttat cccggcggtg cggttatcga aatctacgat gcccttttcc aactcaataa
      661 attcaagcac attctgaccc gtcacgagca ggcggcagta cacgcggcag atgcgtatgc
      721 gcgcgtcagc ggtaaggtgg gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc
      781 actgaccggt attgctactg cctatacgga ttcgattccg atggtggtca tcagcgggca
      841 ggtaggcaat tccctgattg gtacggatgc gttccaagaa gttgatacgg tgggtattac
      901 ccgtccgtgc gtcaaacaca atttcctggt tacggacatc aatgagttgg cggaaaccat
      961 taaaaaggcg ttccaaattg ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc
     1021 gaaagatgtt acgcaggcga tggcgaaatt cagctatcct caggaagaca tttttatccg
     1081 ttcgtatcaa ccggttgttc aagggcatat cggtcagatt aaaaaggccg tgcagatgtt
     1141 ggcatctgcc aaacgtccgg tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc
     1201 tgaagagctg acccgatttg tccgaatgac gggtgctccg tgcacgggta cgctgatggg
     1261 cttgggcgct tatccttccg gcgaccgcca attcctcggt atgctcggta tgcacggtac
     1321 ttacgaggca aaccttgcca tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt
     1381 tgacgaccgt gtggtttccg taccgtccaa atttttcgag aaggcgaaga aggttatcca
     1441 tatcgatgtc gatccttcca gcatcgccaa acgcgtgaag gcggacattc cgattgtcgg
     1501 cgacgtgaaa aacgttttgt ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc
     1561 gtctgaagat gctttgggca aatggtggaa aaccatagag gaatggcgtt cccgcgattg
     1621 cttgtggttt gacaacggca gcgaaattat caagccacaa tatgtgattc agaagcttgc
     1681 cgagattacc ggcaattcgg caatcatcac atcggatgta gggcagcatc aaatgtttgc
     1741 ggctcaatat tatcccttcg aacgtccgcg ccaatggctc aactccggcg gtttgggtac
     1801 gatgggcgtg ggtctgcctt atgcgattgg tgcaaaactt gccgccccgg atcaagacgt
     1861 attctgtatc accggcgacg gctcgatcca gatgaacatc caagagctgt ccacctgttt
     1921 ccaatatcgg attccggtta acgtcattac actgaacaac ggttatctcg gtatggtacg
     1981 ccagtggcag gaaatatatt acggcggacg agagtcggaa acctatttcg attctttgcc
     2041 cgatttcgtc aaacttgccg aggcatacgg ccatatcggt atccgcgtgg acaagaagtc
     2101 tgatgtggaa ggtgccttgt tggaagcatt gaaccaaaaa gacagtctgg tgtttatcga
     2161 cttcctgacc gaccagaaac agaatgtgat gcctatggtt ggcaacggca aaggtttgga
     2221 cgaaatggta cttccgccgc atatgcgtgc ggacggaaag gcgtaaggag agacaaatgc
     2281 gacatatctt atctgttctg atcgaaaacg aatcaggtgc gatgagccgc gtggtcggtt
     2341 tgttctctgc acgcgattac aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa
     2401 ccctgtcccg tatgaccatc gttacacacg gcgacgagca ggttatcgaa caaatcacca
     2461 agcaactcaa taaattgatt gaagtgatta aagtggtcga tttgaatgaa agccgttttg
     2521 tcgaacgcga actgatgttg gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt
     2581 tacgcttgac cgaaatctac cggggcagca tcatcgacgt aaccgaccgc agctatacga
     2641 ttgaaattac cggctcgacc gacaagctgg attccttttt ggaaacggtc ggacgcgccc
     2701 aaattttgga aaccgtacgt acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga
     2761 aaatttaacg ccgtaacctt tcagacggca cggtatttga atgccgtctg aaaaacgaac
     2821 ggcaggagag atttatgtcg aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat
     2881 acacagaaac actggtagca cagtttaaag aactggtcaa agccagccgt gcagaagagg
     2941 gcaacatcag ctacgatctc catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg
     3001 aaaattggaa atcccaagca gctattgacg aacataacgc cagcgcgcat ttccaagcct
     3061 tcgtccagtc cgtcgacggc aaaaccgaag cgttggaaat cgtattgatg aatgaagttg
     3121 ccgattaagc ggtacactct gtttaacccg tccgaagccg ctcaacattt taaggcttcg
     3181 acaactattt accttacaat catttacctt aaaggaaatc aaatgcaagt ctattacgat
     3241 aaagatgccg atctgtccct aatcaaaggc aaaaccgttg ccatcatcgg ttacggttcg
     3301 caaggtcatg cccatgccgc caacctgaaa gattcgggtg taaacgtggt gattggtctg
     3361 cgccaaggtt cttcttggaa aaaagccgaa gcagccggtc atgtcgtcaa aaccgttgct
     3421 gaagcgacca aagaagccga tgtcgttatg ctgctgctgc ctgacgaaac catgcctgcc
     3481 gtctatcacg ccgaagttac agccaatttg aaagaaggcg caacgctggc atttgcacac
     3541 ggcttcaacg tgcactacaa ccaaattgtt ccgcgtaccg acttggacgt gattatggtt
     3601 gctcccaaag gtccgggtca taccgtacgc agtgaataca aacgcggcgg cggcgtgcct
     3661 tctctgattg ccgtttacca agacaattcc ggcaaagcca aagacatcgc cctgtcttat
     3721 gcggctgcca acggcggcac caaaggcggt gtgattgaaa ccactttccg cgaagaaacc
     3781 gaaaccgatc tgttcggcga acaagccgta ttgtgcggcg gcgtggtcga gttgattaaa
     3841 gcaggttttg aaaccctgac cgaagccggt tacgcgcctg aaatggctta cttcgaatgt
     3901 ctgcacgaaa tgaaactgat tgttgacctg attttcgaag gcggtattgc caatatgaac
     3961 tactccattt ccaacaatgc ggagtacggc gaatacgtta ccggccctga agtggtcaat
     4021 gcttccagca aagaagccat gcgcaatgcc ctgaaacgca tccaaaccgg cgaatacgca
     4081 aaaatgttta tccaagaggg taatgtcaac tatgcgtcta tgactgcccg ccgccgtctg
     4141 aatgccgacc accaagttga aaaagtcggc gcacaactgc gtgccatgat gccttggatt
     4201 actgccaaca aattggttga ccaagacaaa aactgattgt tttcaaacgg gactgcctac
     4261 acatcgtgta ggcagt
//
LOCUS       DAWKQJ010000156         4252 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000156 DAWKQJ010000000
VERSION     DAWKQJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.156"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..191)
                     /locus_tag="WJL20_001752"
     CDS             complement(<1..191)
                     /locus_tag="WJL20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ7683871.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAV"
     gene            complement(319..1569)
                     /locus_tag="WJL20_001753"
     CDS             complement(319..1569)
                     /locus_tag="WJL20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ7683872.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1636..1831)
                     /locus_tag="WJL20_001754"
                     /pseudo
     CDS             complement(1636..1831)
                     /locus_tag="WJL20_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2050..3825)
                     /gene="ggt"
                     /locus_tag="WJL20_001755"
     CDS             complement(2050..3825)
                     /gene="ggt"
                     /locus_tag="WJL20_001755"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ7683873.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3993..4229)
                     /locus_tag="WJL20_001756"
     CDS             complement(3993..4229)
                     /locus_tag="WJL20_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ7683874.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENLEIRSTLPLIANRPTFPQ
                     LWVNGKLIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1058 a          998 c         1024 g         1172 t
ORIGIN      
        1 acggctttga acatggacag caggggatag gcaggacgac cgcggtggtc tctgaggtaa
       61 cgggttcttt gacggttcag gtattgttcg atcggctgcc aatcaatcac ctggtccaac
      121 ttcaatagtg ggaagcggtc gatgtgtttg gcaatcatgg cttgtgcggt ttgccggaag
      181 aaggtgctca tgagaaatct cctaaatgtc ttggtgggaa tttaggagat tttgggggat
      241 tttgcaaagg tttccgcctg aaacattatg agatttcagg cggcattgga ttgcttggcg
      301 gaatattttt aaaaaggctt acgcgccgta aacggggtat ttattgcaca aagcagttac
      361 ttgttcgcgg actttggcga ggttggcttc gtcttcgggg ttagacaata cgtcggcaac
      421 caagttcgcc aatacgcgcg cgtcggcttc gttaaaaccg cgtgtggtca tggcggcgga
      481 accgatgcgg atgccggagg taacgaaggg tttttccgga tcgttcggaa tggcgttttt
      541 gttgacggtg atgtgcgctt tgcccaaagc ggcttcggcg gctttgccgg tgattttcat
      601 cggttgcagg tcgacgagga aaacgtggct ttcggtgcgg ccggaaacga tgcgcaaacc
      661 gcgtttgacc agttcttccg ccatggcggc agcattgatt ttcacttgtt ttgcgtattg
      721 tttgaactcg ggttgcaacg cttctttaaa cgctacggct ttggcggcga tgacgtgcat
      781 cagcggaccg ccttgcaggc ttgggaagat ggaagagttc aacgcttttt cgtgggtatt
      841 gtcgcggcac aaaattacgc cgccgcgagg accgcgcagg gttttgtggg tggtggtggt
      901 aacgaagtcg cagaacggca cgggattggg atattcgccg ccggcaacca gaccggcata
      961 gtgcgccata tcgacaaaga ggtatgcgcc gactttatcg gcgatttcgc ggaattttgc
     1021 ccagtcgatt tgcaacgcgt aggcagacgc accggccaca atcattttag gtttgtgttc
     1081 gagagccaag cgttccactt cggcataatc gagcacttcg ttttcatcca aaccataagt
     1141 aacggcattg tagagtttgc cggaaatatt aacgctcgcg ccgtgggtca ggtggccgcc
     1201 gtgcgccaaa gacataccca aaatggtgtc gcccggtttt aaaacggaag cgtacacggc
     1261 ttggttggct tgcgagccgg agtgcggttg aacgttggcg tattgcgcgc cgaacagttt
     1321 tttgacgcgg tcgattgcca attgttcgac aatatcgacg tattcgcagc cgccgtagta
     1381 gcgtttgccg gggtagcctt cggcgtattt gttggtcagc tgggaacctt gcgcttccat
     1441 tacggcgcag ctgacgtagt tttcagaggc aatcagctcg acgtggtctt gctggcgttg
     1501 gtcttcttgg gcaatggctg ctgccaaatc ggggtcatat tgtgcgaggg taacgctttt
     1561 tgaaaacatg ttctcggctc ctttgtgtaa tcagggtatc atgagtgttt tttgtataaa
     1621 aaaatacttc aaaacctaag gcagatagtc cataatgcgt aaattttctt tggcattatc
     1681 aggtaattta tttaacatgc tggtttttag cgtctcatta cctttatgtt agtgcaaggc
     1741 tacccaaagg caatgcatca aatggtaaat tttcggcagt ttgtgcaaaa tcaatcaaat
     1801 aaccagcacc aaccacatct ttatcagcca taacccgctg atacaccact tgccaagaca
     1861 actctcctgc cccaccatgt cttaaaatca gatgaatggc ttgtaatgct gtgtttggcg
     1921 tctcacaaat tgcgaagatc tgctcagaag cttgcttgat ggcttgtatt tttgtttgat
     1981 tactcacaat ttccccctat tttaatatta acttaatgcg gtcaaattca caaaatacaa
     2041 gcttcacctc taatcaccca tcactcgacc ttctcggcgt ggatcggcac caccaaccag
     2101 cctgcttggc tcgataataa tggcttgaac acctgaattt agctcacgca cgtcagtctt
     2161 atagcccaaa tcatttaatg cttgttgcca ctggagggcg gttgtacccg tttctagttc
     2221 atagctacca aagcgattta ataaattggg tgcactgatg gcatcttgga tatccatatt
     2281 ccagtcacta tgtgccacaa tcgtcttagc gacatagcca atgatacggc taccacctgg
     2341 ggagccgatt gccatataag gcttgcctgc tttaaatacg atggttggtg ccattgagga
     2401 gcgtggtctc ttgccgggct cgacacgatt ggcgacctgt ttgccctgct ttattggctc
     2461 aaaactaaag tctgtcagct cattattcag caggtagcca tttgccatca aagttgagcc
     2521 aaacgcattt tcaatggaag tcgtcattga tagcacattg cccgccttat ccacaattga
     2581 tatatgactg gtagaaggta actcaatcgc ttgtgaggac acccactcat gaataaaatc
     2641 gcctgcagat acgctaggca atgccttatc cgactgctca agcagctggc tgcgatgttt
     2701 taggtagtct ttagaaatca actggcgaat gggtactggt acaaaatcag ggtcgcccaa
     2761 atatacatca cgatccgcaa acgcaagcct agaagcgtcg cccaagagac gtaaaccttc
     2821 agcatcatac cccacctgat tgggtgaaaa ttcatttaaa atccccaaaa tctgacccac
     2881 agcaatccca cctgagcttg gtgcacccat accgcatact tcataaatac gataagtcac
     2941 acaaacaggc gggcgttcca ccacttgata atcagataaa tcttgtaagg ataattgacc
     3001 ggggttatcc ttagcatttt ggacaactga aacgatattt tgggcatatt taccagtatg
     3061 cagagctttt gcaccttgag ctgctaacgc ctgaacactg tcagcaaatt ctaaattttt
     3121 cagcaagctg cctgcttgta gcggcacacc attcggcaaa aaataagcgg ctgtttttgg
     3181 atagcgtgcc aaatgctgct gattttgctc aaccgagatg gcaagccttg gcgacacctc
     3241 aaagccttgt tttgccaagc ggatcggtgt atcaaataat tttccccaag gcaatacacc
     3301 gtatcgctga tgtattgtct ccatcagttt agggatagca ggcgtaccca ccgagcgacc
     3361 accgaccacc gcttccataa atttcaatgg ttgaccatct ttatccaaaa ataattccgg
     3421 cgtcgcacgc atcggtgccg tctcacgccc atcaaatgtg gtcaatgttt tggcggtatt
     3481 atcccaatac aacacaaatg caccaccgcc caagcctgac gactgtggct ctaccaagct
     3541 tagtgtcgtc tgcaccgcca ccatcgcatc tgcagcgcta ccgccttgct ttaagatatc
     3601 atagccagct tgtgttgcta atggattggc tgacgctacc ataaaatcac ttgcaatcac
     3661 ctgcttttgt tcggtcagtc ccgttgcatg ttcaggcgtg tgagcgtctg cacctgtaat
     3721 gacagcagaa tgagtattaa ccttaccttg attggcatgg atgacttgac atccagagat
     3781 tgtcatagac attatcaatg cagtcaataa atatgtttta gccacaagca ctccttcgcc
     3841 tgagtttgat tgatgattca tacaaggcat gctgattatt gtatttaata tggctaaata
     3901 attcaatcca aactatcaat cttgaccatc aaaaaaagac cgctaatgtc atcagcagtc
     3961 ttttttgata tttattttaa gatattaagt aatcagacct ttgggctatg ctcttcaatg
     4021 agtggtttta gctcacctga ttggtacatt tgtaggataa tatcactacc accgattaac
     4081 ttaccattaa cccaaagctg tggaaaggtt ggccgattgg cgatgagtgg tagagtactg
     4141 cgaatttcta ggttttctag gatattaaca aaagcaaagg gtctgccaat tgggtcagca
     4201 cctctactgc acgcgctgaa aatccacatt ggggaaactg gggcgtgcct tt
//
LOCUS       DAWKQJ010000157         4151 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000157 DAWKQJ010000000
VERSION     DAWKQJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.157"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..142
                     /locus_tag="WJL20_001757"
     CDS             <1..142
                     /locus_tag="WJL20_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ7683875.1"
                     /translation="VGIGGGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRHI
                     QT"
     gene            272..1708
                     /locus_tag="WJL20_001758"
     CDS             272..1708
                     /locus_tag="WJL20_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ7683876.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAISLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1719..2702
                     /locus_tag="WJL20_001759"
     CDS             1719..2702
                     /locus_tag="WJL20_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ7683877.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2699..3892
                     /locus_tag="WJL20_001760"
     CDS             2699..3892
                     /locus_tag="WJL20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ7683878.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSAKGYQRSAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT          888 a         1348 c         1064 g          851 t
ORIGIN      
        1 cgtcggtatc ggcggcggcg cgctgctggg gcattgggtt acgcaatatg tcggcatttc
       61 ccgtatcggc atcgccggta tgcttgcggc ggcggccggg ctgtggctgt gcctgaactt
      121 gaaccgccat atccaaacct gaccgcgcga tgccgtctga agcccctccc gcccttcaga
      181 cggcattttg attgaagaaa tatccgcccc tgcttaaaat aacgggcttt gccgtgtttt
      241 aacgcctatt ttcttattcc aaggatggtt tatgccgctg ctgtctgtcg agttcgcact
      301 gttctttctc gccttcctgc cgatttactg gggcttggcg aaatacccgt ccgtccaaaa
      361 cctgctgctt ttggctgccg gtatgggctg gctctaccat atcggccccg tatttgcggc
      421 aatcgtcgtc ctttattcct cctgcgtgta ccttttgggc gaactgctcc gttccgatcg
      481 cgaaaatacg cgccgctttt ggctggggtg cggcattgcc atctcgctga ccgtcttggg
      541 ctttttcaaa tatttcgatt ttttccgccc gctgattgca caatatgccg gacaaggcgg
      601 cgcaatcgac atcctgatgc cgctggggct ttcgtattac accttccagt caatcgccta
      661 tttggtttac tgcttccgtg cgccgcacgc cgcgcgtttc gagtggcacg aactgctgct
      721 gcacctgagt tttttcccca ccgttacctc cggcccgatt atccgcgccg ccgcattcaa
      781 aagcgcggac ggcgagcagg cgggcgcatt ggcgcaaatc cgtacccacc aaccacgctc
      841 acccgtccgc cccgcactcg ccgtttccct gattctgctg ggtattgcca aaaaatggtg
      901 gctggcgggg atgctcgcag aaaactgggt atcgcccgta tttgaaaatc ccgcccaatt
      961 cgacggctgg ggcgtattgg cgggcgtgta cggctatacc ttccaactct ttttagactt
     1021 ttccggatat tccgacttgg ttatcggaat ggcaatgctg ctgggcttcc ggctgcccaa
     1081 aaacttctcc gcaccgcttc gtgccgcgaa tatccgcgca ttttgggaca aatggcacat
     1141 cagcctttcc acctggatac gcgactacat ctacatcccc ttgggcggca gcaaaaaagg
     1201 ctttttgcgg acacagttca acctgatggc ggcaatggtg ctctcaggca tctggcacgg
     1261 ctacggctgg aacttcctca tttggggtgc actacacggc acggcgctgg tgctgctcaa
     1321 cacgggcgac cgctatttcg gacgcaacgc actatgccgt ctgaaatacc ttgcgccgct
     1381 ctcgtggttt gttaccttcc atttcgtctg cctgaccttc gtcgtcttca ataccgcaaa
     1441 ccccgacgat gccggcgcgg ttttcaatgc cctctttgcc aatgccaacg gctggaatgc
     1501 gccgcaacag gcggatatgc tgctgcttgc ctcgtttgca tccgtgatgc tgctctaccc
     1561 ttacctgcaa cgcgctttcg acggcgcggt caaaggtttg gaaaaaatcc cgatgtggct
     1621 gtggtttatc cccgtttccg ccgtcctgct gctgattatc gtattggccc cctcggggat
     1681 acccggcttt atttatgcca atttttaagg gtttggacat gaaaaacttt ctttcccttt
     1741 tcgcctccat actgatgtct gccctgattg ccgtgtggtt cagccaaaac cccatcaacg
     1801 cctactggca gcagacctac caccgcaaca gtccgctcga accgcttgcc gcctacggat
     1861 ggtggcggag cggtgtggcg ttgcaagaaa acgcctacgc cctttcagac ggcatcaaag
     1921 ccttcctgtc cggcgaaacg ccgccgacgg ctcaagacgg cggttcggca gatatgccgt
     1981 ctgaagccgc cgcatcagaa gccgtccctc aaaccggtga aacagaatgg aaacaagaca
     2041 ccgaagccgc cgccgtccgc agcggcgaca aagtcttttt tgccggcgac tcgctgatgc
     2101 agggcgttgc acccttcgtg caaaaaagcc tgaaacagca atacggcatc gaatccgtca
     2161 acctcagcaa acaaagcacg gggctgtcct acccctcatt cttcgactgg ccgaaaacga
     2221 ttgaagaaac cctgaaaaaa catcccgaaa tcagcgtgct cgccgtcttc ctcggcccga
     2281 acgacccgtg ggatttcccc gtcggcaaac gatacctcaa attcgcttcc gacgaatggg
     2341 cgcaagaata cctgaaacgc gtcgaccgca tccttgaagc cgcacacacg caccgcgtcc
     2401 aagtcgtctg gctcggcatc ccctatatga aaaaagccaa gctcgacggg cagatgcgct
     2461 acctcgacaa actgctttcg gaacacctgg aaggcaaaat catcctgatt cccaccgcgc
     2521 acaccctgag cggcggggaa gaccgctaca ccgactccgt caacgtcaac ggcaaacccg
     2581 tccgctaccg cagcaaggac ggcatacact ttaccgccga aggacaaaaa ctgctggcgg
     2641 caaaaataat ggaaaaaatc gtttttgaac caagtacgca accatcaagt acacagccat
     2701 gaaccccaaa cacctcatcg cattttccgc cctgttcgcc gccacgcagg cagaagccct
     2761 gcccgtcgcc tccgtcagcc tcgacaccgt taccgtttcc ccgtccgccc cctacaccga
     2821 tacaaacggg ctcttgaccg actacggcaa cgccgccgcc tcgccttgga tgaaaaaact
     2881 ccgatccgtc gcacaaggca gcggcgagac cttccgcatc ctgcaaatcg gcgactcgca
     2941 taccgccggc gacttcttta ccgacagcct gcgcaaacgc ctgcaaaaaa cttggggcga
     3001 cggcggcata ggctgggttt accccgccaa cgtcaaaggg cagcgcatgg cggccgtccg
     3061 gcacaacggt aactggcaaa gcctcaccag caggaacaat agcggagact tcccgctcgg
     3121 cggcatcctc gcccacaccg gcagcggcgg cagcatgacc ctgaccgcat cggacggcaa
     3181 gaccggcaaa cagcgcatct ccctgtttgc caaacccctg ctcgccgaac aaaccctgac
     3241 cgtcaacggc aacaccgtct ccgccaacgg cggcggctgg caggtactgg atacccgcgc
     3301 ggcactgccc ttgaccatac agaccgaaat gccatgggac atcggcttca tcaacatcga
     3361 aaatcccgcc ggcggcatta ccgtttccgc gatgggcatc aacggcgcac aattgaccca
     3421 gtggtcgaaa tggcgtgccg accgtatgaa cgaccttgcc caaaccggcg ccgatctagt
     3481 catccttgcc tacggtacca acgaagcctt cggcgacaac atcgacattg ccgataccga
     3541 acagaaatgg ctggataccg tccgccaaat ccgcgacagc ctgcctgccg ccggcatcct
     3601 catcatcggc gcacccgaat ccctgaaaaa cacgctcggc gtatgcggca cacgccccgt
     3661 ccgcctgacc gaagtccaac agatgcagcg gcgcgtcgca cggcaggggc agacgatgtt
     3721 ctggtcttgg caaaacgcga tgggcggcgt ttgcagtatg aaaaactggc tcaaccacgg
     3781 atgggccgcc aaagacggcg tacacttctc cgccaaaggc taccaacggt cggcggaaat
     3841 gctcgccgac agcctcgaag aactcgtccg cgccgctgca atcaggcaat aatcggacag
     3901 gaggcggacg gtatttccgc aacaggggga tgccgtctga aacgcatacc ttcatattgc
     3961 ttcagacggc atagccaccc cgcgcacggt ttgccggacg caaccggcat tcgcctcaag
     4021 catcggaagg acgggggcga acctccggca tacggcgcaa aggcggcgtt tgatatgccg
     4081 tctgaaggca aagatgataa actgccgcct tccgttttca gacggccttg gattcggatt
     4141 tcaagtgcaa c
//
LOCUS       DAWKQJ010000158         4021 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000158 DAWKQJ010000000
VERSION     DAWKQJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.158"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..939)
                     /locus_tag="WJL20_001761"
     CDS             complement(142..939)
                     /locus_tag="WJL20_001761"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ7683879.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1094..3943
                     /gene="uvrA"
                     /locus_tag="WJL20_001762"
     CDS             1094..3943
                     /gene="uvrA"
                     /locus_tag="WJL20_001762"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ7683880.1"
                     /translation="MCNHHPKHSHGNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYSFDVQAAWEILPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITDRLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSITGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDSGGRIIAQGSPEEVAKVKGSYTGKYLKVVLG"
BASE COUNT         1010 a         1204 c          986 g          821 t
ORIGIN      
        1 tgataaatca ccgtaacgtt aaatttccag attcccattt tcgtgaacat aacggtgtga
       61 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
      121 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
      181 agccgccgct gcctcgattt gccgttgttc ggcagatggg tttgccgctg tttcaaccgg
      241 agcagtttgt aaagcaggct cagattcagg ctcggattcg atttgcggcg cagtcagcgg
      301 caaacgcgcc aatacagtgc tgacaccgtt tactttatcg ccaatcgcca cttgcgcctg
      361 cgcatcgaca ggcagataca tatccacgcg cgagccaaaa cggataaagc catagcgttc
      421 gccgcgagac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg ccaccaaacc
      481 ggccacttga acaaaagtaa tttcacgacc ggaagccgta gtcgccaaca ccgcattacg
      541 ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt tgttatagac
      601 cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga acacgttcat
      661 aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc gctcgaccac
      721 cacgatacgg ccgtcaaccg gactcaacac tgcttcagga ttttgcggaa tctcacgcgc
      781 agggtcgcgg aaaaactgca gggcaaatac ggtaaacacc caaaacggca aagaccacca
      841 gccgcaacac atcgacacca gcaggctcaa agccaaaccg ccgccaataa tcggccagcc
      901 ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt ttgttcaagc
      961 gggttaaaat tgccgacatt atagcggaat ctgccgtact ccgccgacaa atgccgtctg
     1021 aacgcggctt cagacggcat ccgagaaagt gctacaatat cggttttcct ttatttttca
     1081 ggcggcatcc attatgtgca accatcatcc caaacattcg cacggcaacg acaccatccg
     1141 catccgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttggacattc cgcgccacaa
     1201 gctcgtggtg gtaacaggat tgtcgggcag cggcaaatcg tcgctagcgt ttgatacttt
     1261 atatgccgaa ggccaacgcc gttatgtcga gagcctgtcc gcctatgcgc ggcagttttt
     1321 gcagatgatg gacaaacccg atgtcgattt gatcgaaggc ctgtcgcccg cgatttccat
     1381 cgagcagaaa tccaccagcc acaatccgcg ttccaccgtc ggcacggtta cggaaatcca
     1441 cgactatctg cgccttttgt acgcacgcgt cggcacaccc tactgccccg aacacaagct
     1501 gccgctatcc agccaaaccg tatctcaaat ggttgatgcc gtcttaaaac tgccggaaga
     1561 cacgcgcgtg atgattctgg cgccggcggt acgagagcgt aagggcgagt ttgtcgattt
     1621 ctttgccgac ttgcaggcgc agggttttgc gcgcgtgcgc gtggacggcg aagtctatca
     1681 gttggacgaa gtgccgaagc tggaaaaaaa catcaagcac aatatcgacg tggtcatcga
     1741 ccgtgtaaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg aaaccgcgtt
     1801 acgccacggc aacgagcgcg cgctggcgat ggagatggac agcggcgaag aacattggtt
     1861 ttccgcgcgt ttcgcctgcc ccgtatgttc gtacagcctg ccagaattag agccgcgcct
     1921 gttttccttc aacaacccga tgggttcctg cccgacttgc gacggcttgg gcaacaccaa
     1981 tttcttcgac cccgaaaaag tggtcgccca ccccgaattg tcgttggcaa cgggcgcgat
     2041 tgacggctgg gacaagcgca accagttcta tttccaaatg attcagtcgc tggcacggca
     2101 ttacagtttt gatgtgcagg ctgcttggga aatcctacct gcaaaaatca aaaaagtcgt
     2161 gttgcacggc tcgggcaaag aagtcattga tttcacttac ctgtctgaac gcggcaccac
     2221 cttcaaccgc agccacgcct ttgaaggcat catccccaat cttgaacgcc gctaccgcga
     2281 aaccgacagc gaaaccgtgc gcgaaaaact gcgtgaatac cagaaccacc gcgcctgccc
     2341 aagctgcggc ggcgcacgtt tgcgcaaaga agcccgctac gtttacgtca gtggcgaacc
     2401 cttgcacgaa gtttccgcct ggccgctcac caaaacccac cgattctttg aaacgctgga
     2461 tttggacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatta ccgatcggct
     2521 cggcttcctg attaacgtcg ggctggatta cctgaatcta agccgctccg ccgaaaccct
     2581 ttccggcggc gaagcccagc gcatccgcct tgccagtcaa atcggtagcg gcctgaccgg
     2641 cgtgatgtac gttttggacg aaccctccat cggcctgcac cagcgcgaca acgaccgcct
     2701 gctcgccacc ctcaaacgcc tgcgcgattt gggcaacagc gtgattgtgg tcgaacacga
     2761 cgaagacgcc atccgcgaag ccgatttcgt cgtcgatatg ggcccgggcg caggcgaaca
     2821 cggcggcaac gtactgattg ccgacacgcc tgaaaacgtc gccaaatgcg aaaaatccat
     2881 taccggacaa tacctcagcg gcaaaaaatc cattgccgtg ccgtctgaac gcacgcctgt
     2941 caatcccgac cggatgctcg tcctcaaagg cgcgcgcggc aacaacctca aaaacgttac
     3001 ccttgagctg ccgctcggtt tgattacctg catcaccggc gtatccggca gcggcaagtc
     3061 caccctgatt aacgacaccc tcgccaaaat caccgcccgc gaactcaacc gcgcccaaga
     3121 agaacccgcc ccatacgacg acatccgcgg tctcgaacat ctcgacaaag tcatcaacgt
     3181 cgaccaatcc cccatcggcc gcaccccgcg ctccaacccc gccacctaca ccggcctgtt
     3241 cacccccatc cgcgaactct tcgccggcgt ccccctctcg cgcgaacgcg gctacaacgt
     3301 cggcagattc tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag gcgacggcgt
     3361 catcaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag tctgccacgg
     3421 caaacgctac aaccgcgaaa cgctcgaaat ccaatacaaa ggcaaaaaca tcagccaagt
     3481 cctcgacatg accgtcgaag aagcccgcga atttttcgac gccgtcccca ccgtatcgcg
     3541 caaactgcaa accctgatgg acgtaggcct cggctacatc cgcctcggcc agtccgccac
     3601 caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ctcgaactct ccaaacgcga
     3661 caccggcaga acgctctata tcctcgacga acccaccact ggcctgcact tcgccgacat
     3721 cgccctgctg ctggaagtca taggccgtct gaaaggcaaa ggcaactcga ttgtgattat
     3781 tgagcataat ctggatgtga ttaaaactgc ggattggatt gtagatttag gaccggaagg
     3841 tggcgatagc ggggggagga ttattgccca aggtagccct gaggaagtgg cgaaggttaa
     3901 ggggagttat actgggaagt atttgaaggt ggttttagga taattttcag attactgaaa
     3961 aatgatattt catagaggtt aaaaatgatt gaatggcgtt ttgatcaagg tagattagat
     4021 t
//
LOCUS       DAWKQJ010000159         3988 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000159 DAWKQJ010000000
VERSION     DAWKQJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.159"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..694
                     /locus_tag="WJL20_001763"
     CDS             32..694
                     /locus_tag="WJL20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ7683881.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGTIRLQFT
                     EGELAELESELPYSLADIYRYWQAVINKPQTVRREGKKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(777..1628)
                     /locus_tag="WJL20_001764"
     CDS             complement(777..1628)
                     /locus_tag="WJL20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ7683882.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     LSMKKQH"
     gene            complement(1699..2829)
                     /locus_tag="WJL20_001765"
     CDS             complement(1699..2829)
                     /locus_tag="WJL20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ7683883.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRIAALEAQFKNALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2999..3895
                     /gene="argB"
                     /locus_tag="WJL20_001766"
     CDS             2999..3895
                     /gene="argB"
                     /locus_tag="WJL20_001766"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ7683884.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
BASE COUNT          973 a          983 c         1163 g          869 t
ORIGIN      
        1 agggcgcaaa acccatttga aaaggaaaat gatggattcc cgaaaattta ccgaagcatc
       61 caaacggcgg ttgagcgaat tgttggatgc caaaagcgaa caaggcaaca cgatgcgttg
      121 cgacgaggtt caaggtttta tgacggcgct gttgagcggg ccggacaaat tgacaccgct
      181 cgactggctg cccgaagtgt tgggcgacga atcgcaattt accgccgccg aacgttccga
      241 aatcgaacgg ctggttttgg caatggcgat ggaaacaacc gccgcgatgt cggataaaaa
      301 actgcccgat ttgtggctgt atgaaaacga agacggcggc agcgattttt acacatggtg
      361 caatgcttat ctttacggtt tggatattgt gccgaccgat tggtttgaag ccgtcgatga
      421 tgaagcgttt gaagagttgt tttatcccat catggcattg ggcggtattt acgacgaaga
      481 ggaaaacggc actatccgtc tgcaattcac agaaggcgag ctggcggaac tggaatccga
      541 gttgccttat tcattggcgg atatctaccg ctactggcag gcagtcatca acaaaccgca
      601 aaccgtccgc agggaaggca aaaaaacagg caggaacgat ccctgtccgt gcggcagcgg
      661 cagaaaatac aaggcgtgtt gcggtaagaa ttgaagcgtt tgtttccatg aaccaaacgt
      721 aaaaatgccg tctgaaaccg gatttccatg tttcagacgg tatttttcac aggcggtcag
      781 tgctgttttt tcatgctgag ccggacaaag ccgacgatac ccaaaacaat catagggacg
      841 ctcaaccatt gccccatcga cagccccaag gtcagcagcc cgagatagtc gtcgggttgg
      901 cgtgcgaatt cggcaatgaa gcggaatatg ccgtagccgc cgaggaagag cgaggcgact
      961 tgtccggtcg gccgctgttt tttagagaac agccaaacga cggcgaacag gcagatgcct
     1021 tcaagtgcaa actgataaag ctgcgaggga tgacgcggca gcataccgta ttgttgcagc
     1081 cattctgccc aaagcggatt gtgcgcggcg gcttcggcat cttcgtaacg cgcctgcggg
     1141 aagcccattg cccaaaatgc gttgatgtcg gtaacgcgtc cccaaagttc gccgttgatg
     1201 aagttgccga tacgtcccga agcgagaccc agcggaacga gcggtgcgac cgtgtccatc
     1261 agtttgagga agccgatgcc gtgtttgcgg ccgaacaacc atatggcaat aactacaccc
     1321 aaaaagccgc cgtggaacga cattccgcct tcccatacct tgaaaatatc aagcggatgg
     1381 gcgaggtagt cggaaaactt gtaaaacagg acgtaaccca aacgcccacc caaaattacg
     1441 cccaaaatgc cccaagtgag gaagtcgtca agcgattctt tggtaaaaac ggacaagcct
     1501 tgcgcgatgc gccttctgcc gagaaaggta aaaagaataa atccgaggat gtagcttagg
     1561 gcataccagc ggacggcaag cgggccgata ctgatgagga cgggatcgaa ttggggatgg
     1621 ataatcataa caggctttcg ttttcaaatg ccgtctgaaa ggcatgatgc ttcagacggc
     1681 atttctgcaa taagggtttc agcgcaaatc gccgatgacg ttgaggatcg cggacaacgc
     1741 ggcttcgccc aggcgcaacg aacgttgacc gttccacccg aagtcgtcgt cgggcaggtt
     1801 ggcattgtct ttgaacggca tttccaacgt ataggcaagg cagttgaaac ggttgccgac
     1861 ccagtttgtt gccaaggtca cgtttgcctg accgggcgca tctttttcgt aaccgtattc
     1921 gtcttggaaa tcggggctgg cgtttaaaag ggcattttta aactgcgctt ccaacgcggc
     1981 gatgcgcgga ttgtagttcg gcacaccttc cgtacctgcg acaaagacaa agggcaaccc
     2041 ttcgtcgccg tggatatcca aaaacaaatc cacgccggtt tccagcattc tttcacgcac
     2101 gaagaacact tcggggcttt tttccaaagt cggattttcc cattcgcggt tgaggttcgc
     2161 accggcggcg ttggtacgaa ggttgcccag tgccgaaccg tcggggttca tattggggac
     2221 gatatagaac gtggcgcggt cgagcagggt gcgtgctgtc gggtcttgcg gatcgagcag
     2281 cctgccgagc agtccttcga caaaccattc cgccatggtt tcgcccggat gctggcgggc
     2341 ggtaatccag attttcaaat cgctttcgac ctgattgcct atggtcagca gattgatgtc
     2401 gcgcccttgc acggtgctgc ccaagtcgtc gatgcggcac aggccgctgc cttgcgcgtc
     2461 gccgaggagg tttaaatgct gttcttcgga gtaaggttcg aaataggcgt aatacacgct
     2521 gttggacagc ggcgtatgat tgacggtcag tacgccgttt tcgtaggaag tcggtacgcg
     2581 gaaccagttg cggcggtcgt atgaggcaca cgcctgatag ccttcccagc ctttcgggta
     2641 ggcggcttct gccgcattgt caaaatgcat gatgcagttt tgatatgccg cgccttgcag
     2701 ccggaagtag aaccattgtg caaaatcgga ggcgttgtcg ggacgcaggg cgaggcggat
     2761 gttggaagga tcggtcaggt ctttgacgac gaccgagccg gcatcgaagc gggtgctgat
     2821 tttaatcatg ggaaagtcct tgctgtcgcc ggtttctcga accggataaa ccgcgatttt
     2881 accgcccgta tcgcaaggct tcaacctgcc cgaaagtctg ccggatgccg tctgaagatt
     2941 gtttcagacg gcgtttggcg ttaacataag ccgaaattgt caacaatagg gagccgttat
     3001 ggagtctgaa aacattattt ccgccgccga caaggcgcgt atccttgccg aagcgctgcc
     3061 ttacatccgc cggttttccg gttcggtcgc cgtcatcaaa tacggcggca acgcgatgac
     3121 cgaacctgcc ttgaaagaag ggtttgcccg cgatgtcgtg ctgctgaagc tggtcggcat
     3181 tcatcccgtc atcgttcatg gcggcgggcc gcagatcaat gcgatgcttg aaaaagtcgg
     3241 caaaaagggc gagtttgtcc aaggaatgcg cgttaccgac aaagaggcga tggatattgt
     3301 cgaaatggtg ttgggcgggc acgtcaataa agaaatcgtg tcgatgatta acacatatgg
     3361 cgggcacgcg gtcggcgtga gcggacgcga cgaccatttc attaaggcga agaaactttt
     3421 gatcgatacg cccgaacaga atggcgtaga catcggacag gtcggtacgg tggaaagcat
     3481 cgatacaggt ttggttaaag ggctgataga acgtggctgc attcccgtcg tcgcccccgt
     3541 cggcgtaggt gaaaaaggcg aagcgttcaa catcaacgcc gatttggtag caggcaaatt
     3601 ggcggaagaa ttgaacgccg aaaaactctt gatgatgacg aatatcgccg gtgtgatgga
     3661 caaaacgggc aatctgctga ccaaactcac gccgaaacgg attgatgaac tgattgccga
     3721 cggcacgctg tatggcggta tgctgccgaa aatcgcttct gcggtcgaag ccgccgtcaa
     3781 cggcgtgaaa gccacgcaca tcatcgacgg cagggtgccc aacgcgcttt tgctggaaat
     3841 ctttaccgat gccggtatcg gttcgatgat tttgggcggt ggggaagatg cctgaagcaa
     3901 agtcggaaaa tgccggcttt ggcggaaaac ccgtttgtcc ggtttctgtt tttggggttt
     3961 cgggcaattt ccaaaccgtc attcctga
//
LOCUS       DAWKQJ010000160         3981 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000160 DAWKQJ010000000
VERSION     DAWKQJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.160"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            152..3265
                     /locus_tag="WJL20_001767"
     CDS             152..3265
                     /locus_tag="WJL20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7683885.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFGYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
     gene            3346..3981
                     /locus_tag="WJL20_001768"
     CDS             3346..3981
                     /locus_tag="WJL20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7683886.1"
                     /translation="MYSEQGINNTINISTTSLTNATQLTVIGNNNSVYIGNNCKIVSS
                     NIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKSKISIGKD
                     CMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVV
                     GSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNEFYK"
BASE COUNT         1449 a          607 c          617 g         1308 t
ORIGIN      
        1 taataaaata gattaagcta ttcttaaatt cagaatattg cttatctata ttaaaaattt
       61 ctaattttta aggttctgat tgaaatcaga accttatttc aactattact ttttactcat
      121 aatcgaatta tatactttag gactttataa tatggctgtt attatatttg ttaacggaat
      181 tcgggctgta aatggccttg ttaaatcatc tatcaatact gcaaacgctt ttgctgaaga
      241 aggactggat gttcatttaa ttaattttgt tggcaatatt actggagcag agcatttatc
      301 ccccccattc cacttacatc ccaatgtcaa aacctccagc atcatagatt tatttaatga
      361 cattccagaa aatgttagct gccgaaatat tcctttttat tctatccatc aacaattctt
      421 caaagccgaa tacagtgccc actataagca tgttttgatg aaaattgaat ctttattatc
      481 tgaagaagat agcattatct tcactcatcc tcttcaactg gaaatgtatc gtttagcgaa
      541 taataatatt aagtcaaaag ccaagctaat tgtacaaatt catggtaact atatggaaga
      601 aatccataac tatgaaattt tggcacgaaa tatcgattat gttgattatc ttcaaacggt
      661 atctgatgaa atgctggaag aaatgcattc ccatttcaaa atcaaaaaag acaaattagt
      721 ttttattcca aacatcactt atcccatttc attagaaaaa aaagaagctg atttctttat
      781 taaggataat gaagacattg ataatgctca gaaatttaaa cgtatctcta ttgttggcag
      841 tattcagcca agaaaaaacc aattggatgc cattaaaatc atcaataaaa ttaaaaatga
      901 aaattacatt ttacagatat atggcaaatc tattaataaa gattactttg aattaattaa
      961 aaaatatatt aaagacaata agttacaaaa ccgtatctta ttcaaaggtg aatcttccga
     1021 gcaggaaatt tatgagaata cagatatcct aatcatgaca tctcaaagcg aaggctttgg
     1081 ttatatattt ctagagggta tggtgtacga tatccctatc cttgcctata attttaaata
     1141 tggagcgaat gattttagca attataatga aaacgcttca gtttttaaaa ctggtgatat
     1201 ttctggaatg gcaaaaaaaa taattgagct attaaataac ccagaaaaat ataaagaatt
     1261 agttcaatat aatcacaatc gcttcttaaa agaatatgca aaagatgtgg ttatggctaa
     1321 atatttcact attcttccgc gcagctttaa taacgtatca ttatcgtctg ctttcagccg
     1381 aaaagaattg gacgaattcc aaaatattac tttttctatt gaagattcta atgatttagc
     1441 tcatatttgg aatttcgagc taaccaatcc tgcacaaaat atgaattttt ttgctttagt
     1501 tggcaagcga aaatttccaa tggatgctca tatccaagga acacagtgta cgattaagat
     1561 agctcataaa aagacaggga atttattgtc gcttttacta aaaaaacgaa atcagttgaa
     1621 tttatcaagg ggatatacct taattgcaga agataatagc tatgaaaaat atattggagc
     1681 aatatctaat aaaggtaact ttgaaattat tgcaaataaa aagaactcat tagttactat
     1741 aaacaaaagt accttagagt tgcatgagat tccccatgaa ctacatcaga ataaattact
     1801 gattgcttta cccaacatgc aaacgcctct aaaaattact gatgataatt taatacctat
     1861 ccaagcctcc ataaaattag aaaagattgg aaatacttat tacccatgtt tcttgccatc
     1921 tggcatattt aataatatct gcttagatta cggtgaagaa tccaaaatta ttaattttag
     1981 taaatattct tataaatata tctatgactc aattcgtcat attgagcaac atacagatat
     2041 atcggatatt atcgtttgca atgtttattc ttgggaactt attcgtgcct cagttattga
     2101 gagccttatg gaatttaccg gaaaatggga aaaacacttt cagacttctc ctaaaattga
     2161 ttatcgattt gatcatgaag gtaagcgttc gatggatgat gtcttttcag aagaaacatt
     2221 tattatggaa tttccgcgta aaaatggtat agataagaaa acagcagcct tccaaaatat
     2281 accaaacagt attgtaatgg agtatccgca gaccaatggt tacagtatgc gcagtcattc
     2341 actgaaaagt aatgtagttg cggcaaaaca ttttcttgaa aaattaaata aaattaaggt
     2401 agatattaaa tttaaaaagc atgaccttgc aaacatcaaa aaaatgaatc gaattattta
     2461 tgagcattta ggcattaaca taaatatcga agcatttcta aaaccacgat tagaaaaatt
     2521 taagcgtgaa gaaaaatatt ttcatgattt cttcaaaaga aataatttta aagaggtaat
     2581 ttttccaagc acttattgga atccaggtat tatttgtgct gcacataaac aaggtattaa
     2641 ggtatctgat attcaatatg ctgccattac tccttatcat cctgcgtatt ttaaatcacc
     2701 aaaatcacat tacgttgctg ataaattgtt cttatggtct gaatattgga atcatgagct
     2761 tttaccaaat ccaacacgag agattggttc tggtgccgca tattggtatg cattagatga
     2821 tgtgagattt tcagaaaaac tgaattatga ctatatcttt ctatctcaaa gtaggatttc
     2881 ttcgcgcttg cttagttttg caattgagtt tgcattaaaa aatcctcaac tacagctttt
     2941 attttctaag catccagatg aaaatataga tttaaagaac agaattattc ctgataatct
     3001 tataatctcc acggaatctt ctatacaagg catcaatgaa tctcgcgttg ctgtaggtgt
     3061 ttattcaact agcttatttg aggcattagc atgcggcaaa caaacttttg ttgttaaata
     3121 tccgggatat gaaattatgt caaatgaaat agattcaggg ttattctttg cagtagaaac
     3181 acctgaagaa atgcttgaga aaacaagccc gaattgggtg gctgtggcag atattgaaaa
     3241 ccagtttttt ggccaagaaa aataattttt aagttaatct ttaaaagtgc aatatttttc
     3301 ttattttaaa tattgcactt tattctttta atcataggaa ctcacatgta ttctgaacag
     3361 ggaattaata atactattaa catatctact acatctttaa ccaatgccac gcaactaact
     3421 gtgatcggga ataataactc tgtatatata ggtaataatt gtaaaatcgt cagctccaat
     3481 attagattaa aaggaaataa tataacctta tttatagctg atgatgtaga aatcatggga
     3541 ctagtatgtt cccttcattc ggactgctct ttacaaattc aagccaagac cactatgggc
     3601 aatggagaaa ttaccattgc tgaaaaaagt aagattagta ttggcaaaga ttgtatgctg
     3661 gcacatggat atgaaattag aaatactgat atgcatccaa tttattcttt agaaaatggc
     3721 gaacgaataa accatggtaa agacgttatt atcggtaatc acgtgtggct tggccgcaat
     3781 gttactatat taaaaggtgt atgtattcca aataatgtag ttgtaggttc tcatactgtg
     3841 ctatataaaa gttttaaaga acctaattgt gtaatagcag gttcacctgc aaaaatagta
     3901 aaagaaaata ttgtatgggg aagaaaaatg tatcattcaa ctatgtatga tgaccctact
     3961 ttaaatgaat tttataaata a
//
LOCUS       DAWKQJ010000161         3967 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000161 DAWKQJ010000000
VERSION     DAWKQJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.161"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..592)
                     /locus_tag="WJL20_001769"
     CDS             complement(<1..592)
                     /locus_tag="WJL20_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7683887.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADGEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDNRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLCRDIHALTGGAFDPTVQALWKLYADYFAAHPDAETPPPEN
                     SVKEALKRVGFGKVSFDDREIRFTEKGMGLSLNGIAQ"
     gene            complement(601..1395)
                     /locus_tag="WJL20_001770"
     CDS             complement(601..1395)
                     /locus_tag="WJL20_001770"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ7683888.1"
                     /translation="MKAYLDLMRHVLDNGTNKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYSNHFEQAAL
                     QLEREPCALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1817..3151
                     /gene="gdhA"
                     /locus_tag="WJL20_001771"
     CDS             1817..3151
                     /gene="gdhA"
                     /locus_tag="WJL20_001771"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ7683889.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3293..3667
                     /locus_tag="WJL20_001772"
     CDS             3293..3667
                     /locus_tag="WJL20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683890.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3711..>3967
                     /locus_tag="WJL20_001773"
     CDS             3711..>3967
                     /locus_tag="WJL20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ7683891.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1080 a         1035 c          925 g          927 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggtaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gcttcagggc ttccttcacg ctattctccg
      121 gcggcggtgt ttccgcatca ggatgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgcctcccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc ggcggcgaag tcaaataacc gtcacggttc agacggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca ttttttccaa acgcgccact tccgccaaaa
      361 ccttgttgac caaatccgcc gcctgtctgt tatccacgcc gaacaggcgc agctccgcac
      421 cggaacccag cgcgacacct ttccagaaaa acacattttc attgcgtttt tcatccccgt
      481 ttcgtttttc accgtcggcg gcaaaaggat tcggcaggaa agaaaccgcc gcgcccgccg
      541 ccgcaacggc ggcaaccgtc aaaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc
      601 ctaaaccgac actgccgcct tgatatgcgg atgagggtcg taaccttcca actcgaaatc
      661 ttcaaatttg aaggcaaaca aatctttgac ttcaggattg attttcatca ctggcaaggc
      721 gcacggttcg cgttccaact gcaatgcggc ctgctcgaaa tggttactgt acaaatgcgc
      781 atcgccaaac gtatggataa actcaccggc ttccaatccg catacttgcg ccatcatcat
      841 ggtcaacaat gcgtagctgg cgatgttgaa cggtacgcca aggaaaatat cggcgctgcg
      901 ttggtaaagc tggcaggaca gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca
      961 aggcggcaat gccatttcat cgaccaaagc cggattccac gccgacacaa tcaggcggcg
     1021 cgaatcggga tttttcttga tttgttccac cacattggcg atttgatcga tatgcctgcc
     1081 gtcgggtgcg ggccagctgc gccattggta gccgtacacc gggcccaaat cgccgttttc
     1141 gtccgcccac tcgtcccaaa tggaaacatt gttgtccttt aggtatttga tattggtatc
     1201 gcctttgaga aaccaaagca gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt
     1261 cagcagcgga aaacctttgc ccaagtcaaa acgcatctga taaccgaata cggagcgcgt
     1321 acccgtaccg gtgcggtctg atttgttcgt accgttgtcg aggacgtggc gcatcaagtc
     1381 caaataggcc ttcatcatca tctttcatca aattaaacgg cgcatattgt aacatttccg
     1441 gccaaagccc gagtccggac aaacgcacac gccgcccccc cggacgataa aacgcagata
     1501 cgggcaggca ggattgttgg caatttcagt ccttttccac agtaaaaccc ggtgggaaaa
     1561 caaaattacc ttgattggaa tcaaaaaatc tagtttaatt acttagaata aaatttcaat
     1621 aatattttta tttacgaaat taatttatga ttattgattg attatcggca acaaacatca
     1681 aacatcgaaa atatggaaaa aataatgtca acaatttttg ccaaatcggg cttggcatca
     1741 gaaaaaaata ggtttatatt cccacctaca aatttgtttt cccattagta cactatcaac
     1801 caaaaggagt atccgaatga ctgacctgaa cacccttttt gccaacctca aacaacgcaa
     1861 tcccaatcag gagccgttcc atcaggcggt tgaagaagtc ttcatgagtc tcgatccgtt
     1921 tttggcaaaa aatccgaaat acacccagca aagcctgctg gaacgcatcg tcgaacccga
     1981 acgcgtcgtc atgttccgcg taacctggca ggacgataaa gggcaagtcc aagtcaaccg
     2041 gggctaccgc gtgcaaatga gttccgccat cggtccttac aaaggcggcc tgcgcttcca
     2101 tccgaccgtc gatttaggcg tattgaaatt cctcgctttt gaacaagtgt tcaaaaacgc
     2161 cttgaccacc ctgcctatgg gcggcggcaa aggcggttcc gacttcgacc ccaaaggcaa
     2221 atccgatgcc gaagtaatgc gcttctgcca agcctttatg accgaactct accgccacat
     2281 cggcgcggac accgatgttc cggccggcga catcggtgta ggcgggcgcg aaatcggcta
     2341 cctgttcgga caatacaaaa aaatccgcaa cgagttttct tccgtcctga ccggcaaagg
     2401 tttggaatgg ggcggcagcc tcatccgtcc cgaagcgacc ggctacggct gcgtctattt
     2461 cgcccaagcg atgctgcaaa cccgcaacga tagttttgaa ggcaaacgcg tcctgatttc
     2521 cggctccggc aacgtggcgc aatacgccgc cgaaaaagcc atccaactgg gcgcgaaagt
     2581 gctgaccgtt tccgactcca acggcttcgt cctcttcccc gacagcggta tgaccgaagc
     2641 gcaactcgcc gccttgatcg aattgaaaga agtccgccgc gaacgcgttg ccacctacgc
     2701 caaagagcaa ggtctgcaat actttgaaaa acaaaaaccg tggggcgtcg ccgccgaaat
     2761 cgccctgccc tgcgcgaccc agaacgaatt ggacgaagaa gccgccaaaa ccctgttggc
     2821 aaacggctgc tacgtcgttg ccgaaggtgc gaatatgccg tcgactttgg gcgcggtcga
     2881 gcaatttatc aaagccggca tcctctacgc cccgggaaaa gcctccaatg ccggcggcgt
     2941 agcaacttca ggtttggaaa tgagccaaaa cgccatccgc ctgtcttgga ctcgtgaaga
     3001 agtcgaccaa cgcctgttcg gcatcatgca aagcatccac gaatcctgtc tgaaatacgg
     3061 caaagtcggc gacacagtaa actacgtcaa tggtgcgaac attgccggtt tcgtcaaagt
     3121 tgccgatgcc atgctggcgc aaggcttcta agcaaacacc gccgctccgc aaacaaaatg
     3181 ccgtccgaac cgcaaatgct gttcagacgg catttcatta tccgcccgtt cgaatcgggt
     3241 gatactattt gtatccagaa ttgttggata gtttgggagt ggagaggaag aaatgattat
     3301 ggaccaatac tatatggagt taaagaataa gttatctaac agaccaatcc tactggataa
     3361 tactaatgat tttctttttg ttttagtaaa tacagtgaaa gctatgatag aaaatactga
     3421 taaaagtcag ctttcagagt tagataaaat tttagacggt gtcacaagtc aagaattgaa
     3481 acttgcctat gatttttgtc aagggaaatt cggtcaagca ggtttttctt atcgtaggca
     3541 cccgaattat ttttatttgt ccagtttaat tgcgactttt ccagaatttg agttgtctaa
     3601 agcagaccga gattacctca aaggaatcat aaattttgat aactaccttt tatatgagtt
     3661 agattagtct ttaatcacct caaaacccta tttattcaaa aggagaaaaa atgattgagc
     3721 tgaaattagt agatgagagc agttttcagg cagtgctgga tttgaaaata tctgaagctg
     3781 atgaacgagc acgttttgtg gctccaaatg tacgctcttt agctgacgca tggctctacc
     3841 gggaaaatga agatgtgttt ccgagggcaa tctattggga taagcaagtg gttggctttc
     3901 tcctgctgga aatagacaag gatgaagcag aatactttat ctggcggata atgattggtc
     3961 agcagtg
//
LOCUS       DAWKQJ010000162         3963 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000162 DAWKQJ010000000
VERSION     DAWKQJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.162"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            489..1445
                     /gene="thiL"
                     /locus_tag="WJL20_001774"
     CDS             489..1445
                     /gene="thiL"
                     /locus_tag="WJL20_001774"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ7683892.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLRDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGITLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTASESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1438..1923
                     /locus_tag="WJL20_001775"
     CDS             1438..1923
                     /locus_tag="WJL20_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ7683893.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILAGVDGWLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1856..2044)
                     /locus_tag="WJL20_001776"
                     /pseudo
     CDS             complement(1856..2044)
                     /locus_tag="WJL20_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2088..3764)
                     /gene="ettA"
                     /locus_tag="WJL20_001777"
     CDS             complement(2088..3764)
                     /gene="ettA"
                     /locus_tag="WJL20_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ7683894.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          807 a          960 c         1101 g         1095 t
ORIGIN      
        1 aggcaacgct gtactggttt aaatttaatc cactatatcg gttgaaactc tgattttaag
       61 gcggtaggat gtgggtttgc ccatagaaag ggaatccttt ctgtatcaag ccctgaaagg
      121 gataattcat acaaattcac gcctttcccc ttcattggga aatggatgga atcgtgccag
      181 atgtgtgcgg cactgtatgc cggatatggt tttatcatca gcccttttcg gttgaaaccc
      241 cgtcagttgc agcgattgag cctaatcggt ggcggaagtt gccgctttgc attcggggtg
      301 gcgtgcagtg cggtgctttg atatgccgtt tgtgtgttga aacagggtgg tcggtgcata
      361 cgggtacggt atggctaagg ctaaaagtga aatacgctga aacactgaat gagccgcttt
      421 attgtttgta cgacctttgc tgccttgcta tgatttaaat tggctttgct cgccggatat
      481 tttgggatat gaaagaattc gacttcatca aacggtattt gcaaacaggc acggataagg
      541 atgtcgtatt gggcataggc gacgatgcgg cgattgtccg cccacgtgag ggtttcgatt
      601 tgtgtttcag tgcggatatg ctgctgaggg acaggcattt ttttgcagat gtcaaacctg
      661 aagacttggc ttggaaggtt ttggccgtca atatttcaga tatggcggcg atgggtgcga
      721 taccgcgttg ggtgctgctg agcgcggcgt tgcccgaatt ggatgaagta tggctggaac
      781 ggttttgcgg tagctttttc ggtttggcaa aaaagtttgg catcacctta atcggcggcg
      841 atacgaccaa gggcgatatg gcgttcaatg taaccattat cggcgaattg ccgaagggta
      901 gggcgttgcg gcgtgatgcg gcggttgcgg gcgacgatat ttgggtgtcg gggcgtgtcg
      961 gtatggcggc ggcggctttg aactgccgtc tgaaacggtg tgtgttgccg gatgatgtgt
     1021 ttgccgaatg cgaacaaaag ctgctccgcc ccgagccaag ggttgggctg gggcttgcgc
     1081 tgttgccgtt tgcccgggcg gcgcaggatg tttcagacgg cctcgcgcaa gatttggggc
     1141 atatcctgac cgcttccggc gtgggggcgg aaatttgggc cgattcgctg ccgtctttat
     1201 ctgtattgaa agatattttg ccccgagcgc aatggctgtc ttatactttg gcgggaggtg
     1261 atgattacga gctggttttt accgcatcgg aaagttgccg cagccgcgtg cttgatgcgg
     1321 cggaacggtg cggcgtgccg gtaacgcgca tcggcaaaat caacggagga tgccgtctga
     1381 aggttttaga tgccgacggc agggaattgg aactacattc tttaggattc gatcattttg
     1441 gctgatttta aacctgactt tgcgtggctg ttgaaacggc cgttgtgttt tttggctttc
     1501 ggtttcggta gcgggctggc tccgttcgcg ccgggcacat tcggcacttt ggcggcactg
     1561 cctttggcat ttgtgttgat attggcgggt gtggacggat ggctgctggc ttttttgtgt
     1621 atcgtgctgt ttatgtgggg catacgcatt tgcgcttatg cggaacgtga aacgggtgtc
     1681 agcgaccacg gtgggattgt ttgggacgag attgttgcca tgctgtttgt gttggcgttt
     1741 gtgccgttca ggtggacgtg gtggctggcg gcatttgttc tgttccgtct gtttgacgcg
     1801 ctcaaaccgt ctcccgtcgg ttggtttgac aagaatctgc acggcggttt gggcattatg
     1861 gcggacgata tggcggctgc ggtgatgacc ttgattgtct tgaggattgc aatgctgttt
     1921 taaacggtgc tgccttgtat agtggattaa attcaaacca gtacggcgtt acctcgcctt
     1981 gccgtactat ttgtactgtc tgcggcttcg ttgccttgtc ctgatttaaa tttaatccac
     2041 tataaaaatg ccgtctgaaa gcctttcaga cggcattgtt tcggaggtta acgcgttacc
     2101 ggtttgtatt tgatgcgttt cggtttcgtg ccttcttcgc cgagtcggcg tttcttgtcg
     2161 gcttcgtatt cctgatagtt gccgtcaaag aacacccatt tggagtcgcc ttcgcaagcc
     2221 aagatatgcg tagcaatacg gtcgaggaac caacggtcgt gcgaaatcac catcacgctg
     2281 ccggcaaatt ccaacaatgc gtcttccaac gcgcgcaggg tttccacgtc aaggtcgtta
     2341 gacggttcat ccagcagcaa tacattgccg ccgctcaaca aggtttttgc caagtgcaga
     2401 cgaccgcgtt cgccgccaga caattgacct gcaattttgc tttggtcgct gcctttgaag
     2461 ttgaaacgcc ccaaatattg gcgggcggga atttcaaact gaccaacctg caaaatatcg
     2521 cggccttcgg caatgttgtc gaacacggtt ttgtcgtttt gcaaaccttc gcggctttgg
     2581 tcaatcaggc tcattttcac ggtttgcccg atttttactt cgccggaatc gggctgctct
     2641 ttgcccgaaa tcattttaaa cagcgtcgat ttacccgcgc cgttcgggcc gatgatgccg
     2701 acaatcgcgc cagcaggcac tttgaagctc aaatcgtcaa tcagcacttt atcgccgaac
     2761 gatttggaaa cattcacaaa ttcaatcact tcgttaccca aacgctcggc aacgggaatg
     2821 aaaatttcct gagtttcgtt gcgtttttgg tattcgtagt tgctcatttc ctcgaaacgc
     2881 gccaaacgcg ctttggactt ggcttgacgg cctttggcat tttggcgcac ccattccaat
     2941 tcctgcttca tcgccttcac gcgcgcggct tcggattttg cctcgttttc caagcgtttt
     3001 tctttctgct ccagccaaga cgagtaattg cctttccacg gaataccatg gccgcggtcg
     3061 agttccaaaa tccattcggc ggcgttgtcg aggaagtagc ggtcgtgcgt taccgcaacg
     3121 actgtgccgg ggaagcgcac gagaaattgc tccagccatt cgaccgattc cgcatccaag
     3181 tggttggtcg gctcgtccag caaaagcata tcgggcttgc tcaacaagag tttgcacaag
     3241 gcaacgcggc gtttttcacc gccggacaaa ttatcgattt tggcatccca ttccggcagg
     3301 cgcagcgcgt cggcggcgat ttccaattcg tgttccgcac cgccgcccgt ggacgaacct
     3361 gccgcaataa tcgcttccaa gcggccctgc tcttctgcca acgcgtcaaa atccgcatca
     3421 ggattggcgt actcggcata cacttcctcc aaacgtttct gcgcggcagc cacttcgccc
     3481 aaaccgcttt ccacttcctc gcgcacggtt ttttccggat caagctcagg ctcttgcggc
     3541 aggtagccga ttttgatgcc gcccatcggc acggcttcgc cctcaaattc cttatccacg
     3601 cccgccataa tccgcagcac ggtggacttg cccgcgccgt tcaaaccgag caggccgatt
     3661 ttcgcgccgg ggaagaaaga aagggaaata tctttaatga tggttttctg cggcggcaca
     3721 accttgctca cgcgcagcat agaatagacg tattgttggg acatggtttt ctcgttttca
     3781 tcaaacaaat ttcagacggc cattttaacc gataatttga tttaagccag tttatccgcg
     3841 aaccggtatt gccaaaatcg ggcaggattc ataaaatccg cttatccctt tgaaattata
     3901 tagacaaaaa aataataatg ataggggatc gccgccccgg caaccatttc ggattttcca
     3961 aag
//
LOCUS       DAWKQJ010000163         3945 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000163 DAWKQJ010000000
VERSION     DAWKQJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.163"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..155)
                     /locus_tag="WJL20_001778"
     CDS             complement(<1..155)
                     /locus_tag="WJL20_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7683895.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVI"
     gene            762..1577
                     /locus_tag="WJL20_001779"
     CDS             762..1577
                     /locus_tag="WJL20_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ7683896.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEED
                     NYSTGVGSCINGFPVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1594..1869
                     /locus_tag="WJL20_001780"
     CDS             1594..1869
                     /locus_tag="WJL20_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683897.1"
                     /translation="MNEGEVVLTPEQIQTLYGYAFRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(1956..3089)
                     /locus_tag="WJL20_001781"
     CDS             complement(1956..3089)
                     /locus_tag="WJL20_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683898.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(3263..3712)
                     /locus_tag="WJL20_001782"
     CDS             complement(3263..3712)
                     /locus_tag="WJL20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683899.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
BASE COUNT         1158 a          862 c          763 g         1162 t
ORIGIN      
        1 ttgataaccg tgcggatttt acggtaaaac agtgctgccg aattgggatg gatacccaaa
       61 atatcggcgg cagaacgggc ggtaacttcc agtacaaaaa aatggagcag ttctttctgt
      121 acttttttct ttaatttgca gtgcgttatc ttcatatttc gagggtaaca tatctgctaa
      181 tgtagtacag ccccaaaaat ataccaaaaa cagcaaaaca aattgtaagg atacgtatag
      241 gctttgtaaa ggtaaattgt gaaaaaagca gttttttaaa cgaatgaaac ggcttcgggc
      301 tgaaatatat gctgatgccc tgttcttccc gtatttctcg tgtgttgtca aagtgcaggc
      361 tgctttgaaa tcggtattgc catctatgaa ccaccacttt gctttatttc agcgggcttg
      421 agatgtgtat aagaatattg ttttgaataa atttaaagaa aatgataatc gttattgacg
      481 atttttaaag gaaagcgtag agtgccaatt ctatgaagca atacggtaag taacaatgaa
      541 aatatctact gcttgggtat agagcatatt tcacaacccg taactattct tgcggaaaca
      601 gagaaaaaag tttctcttct atcttggata aatatattta ccctcagttt agttaagtat
      661 tggaatttat acctaagtag taaaagttag taaattattt ttaactaaag agttagtatc
      721 taccataata tattctttaa ctaatttcta ggcttgaaat tatgagacca tatgctacta
      781 ccatttatca actttttatt ttgtttattg ggagtgtttt tactatgacc tcatgtgaac
      841 ctgtgaatga aaagacagat caaaaagcag taagtgcgca acaggctaaa gaacaaacca
      901 gtttcaacaa tcccgagcca atgacaggat ttgaacatac ggttacattt gattttcagg
      961 gcaccaaaat ggttatccct tatggctatc ttgcacggta tacgcaagac aatgccacaa
     1021 aatggctttc cgacacgccc gggcaggatg cttactccat taatttgata gagattagcg
     1081 tctattacaa aaaaaccgac caaggctggg ttcttgagcc atacaaccag caaaacaaag
     1141 cacactttat ccaatttcta cgcgacggtt tggatagcgt ggacgatatt gttatccgaa
     1201 aagatgcgtg tagtttaagc acgactatgg gagaaagatt gcttacttac ggggttaaaa
     1261 aaatgccatc tgcctatcct gaatatgaag cttatgaaga taaaagacat attcctgaaa
     1321 atccatattt tcataaactt tactatatta aaaaaggaga aaatccggcg attattactc
     1381 atcggaataa tcgaataaac caaactgaag aagataatta cagcactggc gtaggttcct
     1441 gtattaacgg tttcccggta cggtattacc cgtttattcg ggaaaagcag cagctcacac
     1501 agcaggagtt ggtaggttat caccaacaag tagagcaatt ggtacagagt tttgtaaaca
     1561 atccaagtaa aaaataattt aaaggatctt attatgaatg agggtgaagt tgttttaaca
     1621 ccagaacaaa tccaaacctt gtatggttat gctttccgtg gcgataccta tggcggttgg
     1681 cgttatttgg ctaatttggg tgaccgttat gcggataatg ctgccgcaat tgtcggtaag
     1741 gatgcaaact taaatggttt gaatttatgg atgaaaaaag gtgtggaaaa cctatgggat
     1801 gatacggtcg gtaaaaagat ccgtttaatg tgtatttccg ttttttggat tgtggttttc
     1861 aatttgtagc gaatcggatt cggcatatac ggcattgcaa aaagcgtttg actctccaat
     1921 gccgtctgaa aaccggtttc agacggcatt tgcgttcagt gagaaaggtc gcgcctgccg
     1981 cccgaacgtc tcgccgcagc ctctgcataa cggctcacct ctttttccaa attttccaag
     2041 ttcaaaggaa aatcaggcag tttgtctccc tgtttctctt cgcggataat ccgcccgcca
     2101 tccaaatacc acgtctgttg cgcatgatag gtctgtatat ccgccgttac gccatccgct
     2161 ttcaatgcta ccgtcgaaga ttgtgcaata aaaagatttc cgtttttcaa ataatattcg
     2221 aaactctggc gtttttttcc attgtcgaaa ctccaataga ctttttgcgg cagaccgtcc
     2281 gcatcatagc cgaccacaag actgttcgcc ttcatccccc ggggcatcaa ttcccgcata
     2341 ttctgataaa acacagaatc gcgcgagtcc gatgcaatcc ggttgctctc ttcgcggaag
     2401 tcccaaacct tctgctcatc attcgcgaca tcccggtatt tcgccaaata tacctgggcc
     2461 atctgataac acccgaggca atgctcataa acatcttccc cgattttccc gcgccccgac
     2521 gcatcaaata ccgaaccgtc cggttgccaa acaacccgat attctcctgt cgtttcataa
     2581 ttttctccgt gagccgttcc gccgtacaca tttacagaaa acggacgatc gttccgatac
     2641 agatattcgg cattaacaaa cgcttccggc gagcgttgcg aaagcgaaac cgcaaccaaa
     2701 ccgccctcgc cgatatggta atccagccaa acctctttcc catgttcctg ctccgttacg
     2761 tgaaaccatt tcgccttttc tttcaaacga ctgagccgga tagcgagcgc gagataatcc
     2821 ttctccgact gcaacggacc gtcatccaca gctccggcaa gactttcctc cgtcctcatc
     2881 gattccttca cgatgacaac cgccctgtcg gcatttcgga acaggcgggc aagtttcgcc
     2941 acaaaagcat tcggattttc aggtatttca gttgccgtat cgctcaaaaa ccaacgcgga
     3001 ttaatctcat aggcaatacc cgtccccagc caaaaggcaa atacaagggc aaaaaatgac
     3061 aacaatacca gtttgaattt tttaaacata tttatttttc gtttaattcg tttaacagga
     3121 tatatcgatt atatcagacg agctttgatt gtttgatttt taatctaatc tacgcttgct
     3181 gccgtacttt aaaaaaacct atgcaggctg cggcttgtta ctcttgtttg ctttgattca
     3241 aattttcaac taattcaaat gcttaagatc gatattgcct ttgccatttt tcccaattta
     3301 acggtcttcc cgagcgttcc caatcttcta aaaccgacat tttgaacatt tccatttccc
     3361 accaatattc ggcatatgaa cgtgattgat tgggaatgtc ggcttctgcc cgatcggcaa
     3421 aatgcaaaaa atgagaaata ttgcaaacta tattattttc aggaacttct tcggcaaata
     3481 gatgcttgac tgactgtaac gcttcggtct gatatttttc tgtccatttc tcccattttt
     3541 caggctttcc cgatatttcc caatcttcca atgcggaagc attcaagatt tccaattccc
     3601 accaacagtc gtaataacgg gaggattggt taggaatctc attttctgct tcggaaacaa
     3661 attgggtaaa actaaaaata tccttaacga ttttttcaaa tctattttcc ataggttgcc
     3721 gcccctccca attgcgtgcc aacaatttcg gcaaaatcaa gccggcaagc cccggcatcc
     3781 tgtgctaatc ggagaaaatg ccgtctgaaa gccgaatacc gcttcagacg gcattttgat
     3841 tgttgggttt ttaatcagag ctttgtttga taatttgtta ttttgaataa aatacaggtt
     3901 ctgtcgagct gataacttct attacagaat cgctaaaaaa gaaaa
//
LOCUS       DAWKQJ010000164         3881 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000164 DAWKQJ010000000
VERSION     DAWKQJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.164"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..906)
                     /locus_tag="WJL20_001783"
     CDS             complement(88..906)
                     /locus_tag="WJL20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7683900.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WJL20_001784"
     CDS             complement(986..2236)
                     /locus_tag="WJL20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7683901.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPSRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJL20_001785"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJL20_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ7683902.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHLGAVKSPTYAIVESYPLKPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WJL20_001786"
     CDS             2896..3708
                     /locus_tag="WJL20_001786"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ7683903.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          859 a          950 c         1137 g          935 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggcgtg tccattttgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgtcggcg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcagggttg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gtgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaga tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagt gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacgt tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attgttttgc gtctgttcca agaatttggc ggcagagctg gtcgcgcctt
     1381 tggtgtttaa catataaacc cccgtgccgc gcgcggaggg gctggtgaag gcatcggcgt
     1441 ggatggagac aaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaaag
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttaagca gcgccatcat cggatcgtcg ggatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg gtcggcgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatgtcgac gaccagcctg cttgggttgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg ttgtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt acggcggtaa actgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggcgtaaa
     2341 ttccccgccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 aagccccgcg tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcag
     2461 ggtgaagggt ttcagcggat aggattcgac gatggcgtaa gtcgggcttt tgactgcgcc
     2521 cagatgacct aatccgcgca ggatgccgcg tgtcagcgtg gttttacccg cacccaaatc
     2581 cccctcgaga taaatgacca gcggcgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cggattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtacgcgcg ctgatggagc ggctgccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc taccatcgaa
     3061 aatttctcga tgcagattgt cgatttttta ttggaacacg atgttaaggc gatggttatc
     3121 gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac gggcaatatg
     3181 cctgtgttgg acgtgatttc cgccggcgcg aaagccgcgc tggcaacgac gcgcaacaat
     3241 aaaatcggca ttatcgccac caataccaca gttaacagca atgcttatgc gcgcgccatc
     3301 cataggaaca accctgatac gctcgtccgc acgcaggctg caccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacacactt tcccttgctc
     3481 aagcccttaa tcggcaggga ggcgcacaat gtcgcgttgg ttgattctgc aattacgact
     3541 gccgaagaaa ccgcacgcgt ccttgctcag gaaggattgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tggggtagcg gcaggtacgg
     3721 acggaaaccg tccccgtccg cgccatccgc atacgcacct gcaatgccct gccgtattaa
     3781 acgccccgtc tccgcaaatg ccgtctgaaa ggcttcagac ggcatttttt acacaactcc
     3841 caccgtttcc catccttctc gatacactgt aatctcgaaa c
//
LOCUS       DAWKQJ010000165         3878 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000165 DAWKQJ010000000
VERSION     DAWKQJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.165"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            151..654
                     /locus_tag="WJL20_001787"
     CDS             151..654
                     /locus_tag="WJL20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ7683904.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            763..1095
                     /locus_tag="WJL20_001788"
     CDS             763..1095
                     /locus_tag="WJL20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683905.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            1092..1502
                     /locus_tag="WJL20_001789"
     CDS             1092..1502
                     /locus_tag="WJL20_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ7683906.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            1517..2239
                     /locus_tag="WJL20_001790"
     CDS             1517..2239
                     /locus_tag="WJL20_001790"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ7683907.1"
                     /translation="MNCCWGLSFYYQLAVQSEEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPG
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(2558..2920)
                     /gene="ssrA"
                     /locus_tag="WJL20_001791"
     tmRNA           complement(2558..2920)
                     /gene="ssrA"
                     /locus_tag="WJL20_001791"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2983..3588)
                     /locus_tag="WJL20_001792"
     CDS             complement(2983..3588)
                     /locus_tag="WJL20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ7683908.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT          984 a          898 c         1015 g          981 t
ORIGIN      
        1 aattaaaagc cgaaaagaag agtttaaaaa attaatggaa aagaagaaac ccaaaatcta
       61 gaacgagttt taaccccctt taaaatccca ttcagacgac cttttctaaa atccctgtat
      121 tgatttttaa ttcaatacag ggatttttcc atgtcagacg aattcaaccg atttatcgaa
      181 cgcgtcctct cccacgaggg cggttacgtc aatcatccca aagaccccgg cggcgaaacc
      241 aattggggca tcactaagcg caccgcacag gcaaacggct acaacggctc catgcgcgcc
      301 atgacgcgtg aacaggcaat cggcatttac cgtaaagcgt tttgggagcg ttaccgcgcc
      361 gaccaaatgc ccgaagcggt cgcttttcaa ttttttgatg cctgcgtcaa ccacggttac
      421 ggcaatgccg cccgtatgct ccaacgcgcc gcaggcgtgc cggacgatgg aatcatcggc
      481 gaaatcagcc tcaaagccat caattccctt cccgaaaacg accttttatt gcggttcaac
      541 gccgagcgtc tggtctttta taccaagctg ggtacgttca cctctttcgg caagggctgg
      601 gtacgccgtg tggcgcaaaa cctgattcac gccgccgcag acaatgccga ttgaggagca
      661 aaccatgtca aaaaagtcca ttctcgccct gatggccgca gccatgcagc ccgatttcag
      721 ccacagcgac ctaggcattc gctacgccat gccgactcag ggatgttgga cgcaagccca
      781 ccgcaagagc ggggtagccg ccgcgaaacg cgcagccaaa aaaaagcgtc acaaataacc
      841 gcctttttcc gatggctggg cggcttggtc tctaatccgg ccacagggaa aatcagccat
      901 accaaacttt gggcaaacgt cgccgcagcc gccatgactt ggaagttcgt gcaggcggcg
      961 gacgcgcccg aatggctttg gtgggcatac ggcgcgatgg tcggcgggta tgcattaatc
     1021 aaacgcggca tcgccgtcgt accgcagttg gcggaagtca aaaaatccgc aaatcaggaa
     1081 ggggggcggc aatgattgaa tttgtccgag ccaaaaaacg gctgctttgg gcatttgtgc
     1141 ttttgcttgt gtggacgtgc ggttaccgat acgccgccga caaggccgaa gcggaacaaa
     1201 ccgccctgat tgccacctat cggcattctt ctatggttgc ggcggaacaa tatgccttgc
     1261 agcttaaaaa agcgcaggac gaaaggcagc ggtggtacga cttttcccaa aaacaaggaa
     1321 gaaagcccgt gaaaaaacag tatccgccgc aaacgaaaaa agccggctat ctgaaaacca
     1381 aggaagaact gcttgcggaa ttggcttgcc ttaaagcgga aatggttgcc ctaaaaaagc
     1441 ccgatgcctt aatccatggg aaagaagtgc ggcagaaaga acgcaactcg tcgcagggtt
     1501 aaggcaatgc catccgttga actgctgttg gggattgtcc ttctattacc aattggccgt
     1561 ccaatcggaa gaagacaaat atgccgattt gaaacggcat atccatgata tttatcgacg
     1621 acataaggga agatacggct accggaggat tgcggcagcc atccgtcacg caggaacacc
     1681 ggtcaatcac aagaaagtca gccgtctgat ggcgaagacg gggctgaagg cagtgatacg
     1741 gcggcgcaaa taccgctcgt tcaaaggaga agtcggcaaa attgcgccga atatcctgcg
     1801 acgctgtttc catgcagaaa agccgaatga gaaatgggta acggacgtta ccgagttcaa
     1861 tgtaggcgga gaaaagatat acctttctcc gattatggat ttgtttaacg gggaaatcgt
     1921 cagttaccgt attcaaatcc gcccgacttt cgatttggcc ggcgagatac tgaaaggtgc
     1981 gccggagaaa ccgggatcgt ctgaaaagcc gatactgcat tcggatcaag gttggcaata
     2041 tcagatgttt ttttatagtg gattaaattt aaatcaggac aaggcgatga agccgcagac
     2101 agtacaaata gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata
     2161 caacaacgat agaatcaagt tgaaattaaa agggctgagc cttgttcagt acagaattca
     2221 gtccctgaaa gccgcttgat taaactgtcc gactttttgg ggtcagttcg gcttcggcat
     2281 ttttttatcc gtttgggggt aacttgtttg gaaagctgca agcttataaa taaaggatta
     2341 catttaagtt ttgggtaacc tttttaaaaa aatgcgtgat gacttttgca tttttaaggc
     2401 gttttttggg gtaattcgtg aaaagttacc ccaaaagtta ccccataaat ggcgaaaact
     2461 caagcatacg ccagcatatt caaggcaaca aaaaagcctt gaaactgttg aagttcaagg
     2521 cttttttgtg ttgcaggatg ctgctgaaaa tagggtatgg tggaggcggg gggaatcgaa
     2581 cccccgtccg aaagtcctct acaaagcgtt ctacatactt agttgtgtct atttgaaaat
     2641 cttatttcca tcatgccgac caacaggcct tttggaaacc agttacctta aatctcattt
     2701 cctgccaagt aacccggcag gaaaccagtc aatgtaagat gacgttgcgg tggctttcgc
     2761 cacacagccc attgaccgac tgctgcaacg gctagcctta agcggctaaa gcgtaagttt
     2821 cgtcgtttgc gactatttga attcagtgtt ttacgggaat ctgagacccc ggtatgcccg
     2881 catctgcttc gcaacccccg tcgaaaccaa ggtcgccccc agaaatggtt tgcaaattat
     2941 acggatattg tgcggtgctg ccaagtctgt cggagaaatt tgtcagtctt gctgccttaa
     3001 tttgcgtttg agcaggatgc ggacgcagcc gtcgttgcct tcccggggtt cggcgtaggc
     3061 gagtacatcg gggtgttgca tcagccagtt tcgggtcata tttttcagaa cgggtttgta
     3121 gcctttggaa cctaatccgc tgccgtggat gatttcgccg catacgccgc gtttttgggt
     3181 gaatgcgatg aattcgttga ggactttttg ggcttcttcc tgtgtgtagc cgtgcaggtc
     3241 gacatcggta acgacgggat agtatccgtt tttcaggcgt tggatgtcgt tttttccctg
     3301 tccgtttttg ctgaagctgg cgggcgggtc gttgtatgtg ctgccgatgt agaagtagtt
     3361 ttcttcgtcg gcgcggttgt ctttgggacg gactttgatg ggggttttgt cgggcggcgc
     3421 ataatattgc tgccggtttt ttaatgggga gacttgtccg actgcttgtg aaaaatcgaa
     3481 atcctgttct tgtttttgct tgtttttttc ggctgcctgt ttttctgccg cttctttttg
     3541 ggcttgtttg cccagttgtt tgaggatgtt ttggaagtcg gtattcatat tttttcctgt
     3601 tatttgtccg atggctgttt cgggcggggt tttaatttgc cggaatgttt gccaatcggg
     3661 ggaggatgat tttgttgcct gcgtatgttt tttgaaagtg tgattgtata tcaaaaagaa
     3721 atgcggcaac cgtcggcagt gttgattgcc ggaaatgcgg accggtcgaa ccgatatgcc
     3781 cgaacgcctg ataaagtttt aaaaacctgc cttgcgaagc aggctgacgt gttttgccaa
     3841 tcttgaattg ccggaaacgc gaaacacgga aatctgat
//
LOCUS       DAWKQJ010000166         3815 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000166 DAWKQJ010000000
VERSION     DAWKQJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.166"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            180..1697
                     /locus_tag="WJL20_001793"
     CDS             180..1697
                     /locus_tag="WJL20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7683909.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSLAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1756..3537)
                     /locus_tag="WJL20_001794"
     CDS             complement(1756..3537)
                     /locus_tag="WJL20_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ7683910.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          797 a          849 c         1259 g          910 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactataa atgtgaaatc cgccctttga aaatcgggcg
       61 gcggtttttt gcctgcccgc tttcaggcgg caaagccggt tttcacgggt ttctgcctgt
      121 ttttcggatg gtttgacgtg cttcggcggc gtgtttgcca gaaaggtaaa tgacagggta
      181 tgttgtattt cagatacggc tttttggttg tttggtgtgc ggcaggtgtt tctgccgcct
      241 atggggcgga tgcgcccgcg attttggatg acaaggcatt gttgcaggtg cagcggtcgg
      301 tgtcggataa gtgggcggaa tcagattgga aagttgaaaa tgatgccccg cgcgtggttg
      361 acggggattt tttgttggcg catccgaaaa tgttggaaca tagtttgcgc gacgcgctca
      421 acggcaatca ggcggattta atcgcttcgt tggcggattt gtatgccaag ctgccggatt
      481 atgacgcggt tttgtacggc agggcgcggg ctttgctggc gaaattggcg ggaaggccgg
      541 cggaggcggt ggcgcggtat cgggaactgc acggggaaaa tgcggcagac gagcggattt
      601 tgctggattt ggcggcggcg gagtttgacg atttccgact gaagtcggca gaaaggcatt
      661 ttgcggaggc ggcaaaattg gatttgccgg cgccggtttt ggaaaatgtg gggcgttttc
      721 ggaaaaaaac ggaggggctg acgggctggc gtttttcggg cggcatcagc ccggcggtca
      781 atagaaatgc caataatgcc gcgccgcagt attgccggca aaacggaggc cggcagatat
      841 gcagtgtcag ccgggcggag cgggcggcag ggttgaatta tgaaatcgag gcggaaaaac
      901 tgacggcgtt ggcagataat cattatttgt tgttccgttc caatatcggc ggcacgagct
      961 attatttcag taaaaaatca gcttatgatg acgggttcgg cagggcgtat ttgggttggc
     1021 agtataaaaa tgcgcggcag acggcgggga ttttgccgtt ttatcaggtg cagttgtcgg
     1081 gcagcgacgg ctttgatgcg aaaacaaaac gggtaaacaa ccgccgcctg ccgccgtata
     1141 tgctggcgca cggagtcggc gtgcagctgt cccatactta ccgcccaaac ccgggatggc
     1201 aattttcggt cgcgctggaa cattaccgcc aacgctaccg cgaacaggat agggcggaat
     1261 acaataacgg ccggcaggac gggttttatg tttcgttggc aaaacgtttg ggtgaatcgg
     1321 caactgtgtt cggcggctgg cagtttgtgc ggtttgtgcc gaaacgtgaa acggtgggcg
     1381 gcgcggtcaa taatgccgcc taccggcgca acggtgttta tgccggttgg gcgcaggagt
     1441 ggcggcagtt gggcggtttg aacagtcggg tttctgcgtc ttatgcccgc cgcaactata
     1501 agggcattgc ggctttctcg acagaggcgc aacgcaaccg cgaatggaat gtctcgctgg
     1561 ctttgagcca cgacaagttg tcgtacaaag gtatcgtgcc ggcgttgaat tatcgtttcg
     1621 gcaggacgga aagtaatgtg ccgtatgcga aacgccgcaa cagcgaggtg tttgtgtcgg
     1681 cggattggcg gttttgaatg gtgggataat gccgtccgaa ctttggaaac aggttcggac
     1741 ggcatttttg cgcgttcagg caagggcggc ggcaaatacg ccgcgcaggg cgttggagag
     1801 gcggatttct tcggcttctt gcagtgtttt ttgtgtgatg tgtgtttcga ttacttgatt
     1861 tgtttgcaaa tatttttgcg gttcgtccaa tacggcttgg cgcattatgc cgtttaaaat
     1921 gtctaaatct aaagagggcg taagccattg tccgcgatgt ttgacgaaca cgttgcttct
     1981 gccgccttcg agcaggatgc cgtccgaatt gaaaaacagg ctgtcgaacg cgccttgtgt
     2041 ttcggcggtt tgccacgctt ggtcgaagag ggcgcggcag gtggttttga agcggcgcag
     2101 gtagttttgt gcgggcagga tggtcggcga aatgatgacg cgttgtttgt cggtcagacg
     2161 gtttaaaacg gcgcgggaca ggctgatgcc gtctgaagcg agcagggctt tgatgcggaa
     2221 cgcgccgtcg ggcaagcgtg cgatgtattg tttgatttga ttttcgcaac cgtcgggcag
     2281 gggcaggttg agggcttggg cggaggtttt cagacggcat aggtggcggt cgagcagggc
     2341 gcagcgtccg ttttccacgc gcagggtttc aaaaatgccg aagtcggggc gcaattcgtt
     2401 gaggaaacgg gctttccagc cgcattcccg atattcggcg gcagggtcgc tgtcgatgac
     2461 gatgccggaa ccgacaccgt acacgccttg ataaatgccg tctgaaagcg gcgtgagcga
     2521 caaggtgcgg ataacgacgt tgaacgtgcc ttcaaacccc aagccgccgg aacacgggtt
     2581 caaatagccg atgctgcccg tataaagtcc gcgcgcttcg gcttcgagcg attcgataat
     2641 ctgcatactc atttttttgg gcgcgccggt gatgctgccg caggggaagg cggcgcggag
     2701 gatgtcggcg aacgaggtgt gcggcaaggc ttgggcttgg atggtgctgg tcatctgcca
     2761 aacgctgccg aaacgcgata ctttaaacgg ttcgggtacg catactgtgc cggtttgggc
     2821 gattttgccg aggtcgttac gcagcaaatc gacaatcatc acgttttcgg cgcggttttt
     2881 cgggtctgct tgcaactcgg cggcgcggcg ttcgtcttgt ccgtcgccca aaatcggcgc
     2941 ggtgcctttc atcggttcgg tgctgatggt gccgtccgaa ccgattttga ggaagagttc
     3001 gggcgagaaa cacagcgtcc acgcggattg cccttgtgca tcgggcaggt gggacaaaac
     3061 ggcatagggg acgggctggc gcaggcgttg gtaaagtttg acgggattgc cgtaggcttg
     3121 caggtgcagg cgggtagtgt agttgatttg ataggtgtcg ccgcgtcgga tggcttcgtg
     3181 gatttggcgg atatggtcga ggtaatcggc ttcggatacg gaggattgcg gcgtggaaat
     3241 gccggcgggg aggccgtctg agtgtcgggc aagccagctt gcggcatcgg tgtcggcgca
     3301 gtcggcaaac cagtgcaggg caaggttgcc gccgcgttcg gactcgaccc ccgtcagcgg
     3361 caaaccgaat ccgtagtctg caaacaacac ggcatgcagc cctttttgcc agcccgattg
     3421 cagcgcgccg tccaaagcat cgagttcttc gggacggaaa aaacggcttt ccacatgatt
     3481 ttgatagcgt tttgcgcggc cgcttacggc atcgtcaaac agggcgaaat aaggcatggc
     3541 aattcggggc aaatgttttg attatacgct ctttttacac atattttcag acgattcggc
     3601 aaatatcggc aaaatgtaat tttatgtaga gaaagtgggg ggcgaaggtg taaaattgtg
     3661 aaaacaaatt ccgatttcca acctgaaata caataaggag acctttatga cagaccacca
     3721 gctgcaaccg tttgaaaacg tagaattagg cgaaaagcaa gatcagctcc aagtatagtg
     3781 gattaacaaa aaccagtaca gcgttgcctc gcctt
//
LOCUS       DAWKQJ010000167         3796 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000167 DAWKQJ010000000
VERSION     DAWKQJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.167"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..413)
                     /locus_tag="WJL20_001795"
     CDS             complement(<1..413)
                     /locus_tag="WJL20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ7683911.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILIGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            558..1010
                     /gene="dut"
                     /locus_tag="WJL20_001796"
     CDS             558..1010
                     /gene="dut"
                     /locus_tag="WJL20_001796"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ7683912.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            1088..2275
                     /gene="dapC"
                     /locus_tag="WJL20_001797"
     CDS             1088..2275
                     /gene="dapC"
                     /locus_tag="WJL20_001797"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ7683913.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLDPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2537..3316
                     /gene="yaaA"
                     /locus_tag="WJL20_001798"
     CDS             2537..3316
                     /gene="yaaA"
                     /locus_tag="WJL20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ7683914.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3399..3489
                     /locus_tag="WJL20_001799"
     tRNA            3399..3489
                     /locus_tag="WJL20_001799"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3433..3435,aa:Ser,seq:tga)
     gene            3567..3656
                     /locus_tag="WJL20_001800"
     tRNA            3567..3656
                     /locus_tag="WJL20_001800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3601..3603,aa:Ser,seq:cga)
BASE COUNT          985 a          895 c          928 g          988 t
ORIGIN      
        1 ggcatattga atgcgggcaa accggctgct ttccgttttt ggatttcgga gaatgccatc
       61 gcccagcttt catcacacat aaaaaacagt gcgggcacgg cttttttcag cggtatttct
      121 ttcaggtgcg gggcaagtgc cgccccaatc aggatatgcc gcgaattaat cataaaggtt
      181 acggtggcga taagcagtat cggcagagat tccgcccaca ggttgaccgt ggcaaactcg
      241 gagccgccgg cgaagttcat actggtcatc aacaacattt ccagccagct catgcctttt
      301 tgtccgccct gcataccgag tattaatgcc caaggcagaa gtccgataag tatgggcgaa
      361 ctttctttga tgccgcgtat aaattcgtta cggggcgagc ttatgtgtgt catattataa
      421 tattgatatg taagaaggtc gggcattata cacagacgga gtatttcaaa acaaaaccga
      481 tttgccgtgt ttcagcgtaa acacggcttg tgtataatct cccatcttta aaaccggccg
      541 tatgcaggag caagatgatg aatattgaag tagaaatgaa agtgttggac gagaggatgg
      601 cggattttat ccctgcctat gcgacggagg gttctgctgg tttagatttg cgtgcctgtt
      661 tggatgagga agttgtcttg cagccgggtg aaacgtttct tgtaccgaca ggtttggcaa
      721 tttatttggc gaatcccgca tatgccgccg ttttgctgcc ccgttccggc ttggggcata
      781 aacacggcat tgttttgggc aatttggtcg gtttgattga ctccgattat caaggggaat
      841 tgaaggtgtc gttatggaac agaagcagcg aaccgtttgc cgtcaagccg tttgagcgta
      901 tcgcgcagat ggttatcgtt ccaatcgtac aggcgggctt caaacgtgtc gaggagtttg
      961 tcggaagcag ccggggtgag ggcggcttcg gcagtacggg ttctcactaa aaatatagaa
     1021 tgccgtctga aagacacgtc aggttcagac ggcatatctt ccgatatacc gataaccgga
     1081 gaaaatcatg aataccttac tcaagcagct caaaccctat ccctttgccc gactgcgcga
     1141 ggcgatgcag ggcatttccg cgcctgaagg catggaagcc gtccccctgc atatcggcga
     1201 accgaaacat ccaacaccga aagtcattac ggatgcgctg accgcctcat tgcacgagtt
     1261 ggaaaaatat cctctgactg cgggtctgcc tgaactgcgt caggcgtgtg caaactggtt
     1321 aaaacgccgt tacgatggct tgacagtgga tgcggataat gaaattctgc cggttttagg
     1381 cagtagggag gcgttgtttt cttttgttca aaccgtgttg gaccctgttt cagacggcat
     1441 caaacccgca attgtcagcc cgaatccctt ttatcagatt tacgaaggtg cgacactttt
     1501 gggcggcggt gaaatccatt ttgccaattg ccccgcgccg tctttcaacc ccgattggcg
     1561 tagtatttcc gaagaggttt ggaaacgcac caaactggtg ttcgtctgct cgcccaacaa
     1621 ccccagcggc agcgtgctgg atttggatgg atggaaagaa gtttttgatt tacaggataa
     1681 atatggtttc attattgcct cggatgaatg ctattccgaa atctatttcg acggcaacaa
     1741 acctttaggc tgcctgcagg ccgctgcaca gttggggcga agcaggcaaa aactgcttat
     1801 gttcaccagt ttgtccaagc ggtccaatgt tccgggtttg cgttccggtt ttgtcgccgg
     1861 cgatgccgaa ctgcttaaaa actttctgct ttacagaacc tatcacggca gtgcaatgag
     1921 cattcccgtg cagcgcgcaa gtattgccgc ttgggatgac gaacagcacg ttatcgacaa
     1981 ccgccgcctg tatcaggaaa aatttgagcg cgttattccc attttgcaac aggtatttga
     2041 tgttaaatta ccggatgcct cgttttacat ttggttgaaa gtccccgatg gcgacgattt
     2101 ggcatttgca cgcaatttat ggcaaaaagc cgctatccaa gtattgcccg gacgcttttt
     2161 ggcacgggat accgaacagg gcaatcccgg ggaaggttat gtccgtatcg ctttggttgc
     2221 cgatgtcgca acttgtgtca aagctgcgga agacatagtt tcactgtatc ggtaaggaat
     2281 cggaaatgcc gtctgaactt ttgttcagac ggcatttttc aatattctac ggttgaattt
     2341 gctgtaacgg tatttatagt ggattaacaa aaatcaggac aaggcgacga agccgaagac
     2401 agtacagata gtacggcaag gcgaggcaac gctgtactgg tttaaattta atccactata
     2461 cttcactttt aatcggcttg cccgcaaaca cgtttaaact taaaatcccc gtgtttgaca
     2521 caataccgag cagattatgt tttttgtcct ttcccctgcg aaaaacctta acgaaaaaga
     2581 ccctgcccct gtcagcgagt ttacccaacc cgacctgctg gcagagtccg acattctaat
     2641 gcagcagttg cgcgagcttg cgccgcaaca gattgccgaa ctgatgcacg tttccgacaa
     2701 aattgccctc ttaaacgcgc agcgcaatgc agaatggaac acgccgttta cgccggaaaa
     2761 cgccaaacag gcggtcttta tgttcaacgg cgatgtttac gaaggtatgg atgcaaacac
     2821 attggatatt ggacagatac gctatctgca aaaccatgtc cgcctgctgt ccggtctgta
     2881 cggtcttctt cgcccgttag acctgataca gccctatcgt ttggaaatgg ggacggcatt
     2941 tgccaatttg cgcggcaaga atttgtatga gttttggggc gacatcatta ccaacctttt
     3001 aaatgacacg cttgcccaag caggcagcaa tacgcttgtc aaccttgcct cacaggaata
     3061 tttcaagtcc gtcaacacga aaacacttcg ggcgcagctg attaccccga tatttaaaga
     3121 cgaaaaaaac ggtaaatata aaatcatcag tttctatgcc aagcgtgcgc gcggattaat
     3181 ggtgcgctat gcggcagaac atcatattac cgaccctgaa atgctgaaaa attttaatta
     3241 cgaaggctac gcattcaatg acgcggcttc aaatgaaagc gaatgggttt ttatgcgttc
     3301 ggaacaaata aagtgaaaac aataaattaa gtattttccg aaaaaagtgc ttggcaaaat
     3361 gtataaactt cattattatt cttcttcttc aagaagacgg aagcgtggca gagcggttta
     3421 atgcaacggt cttgaaaacc gtcgagggtt gatagccctc cgtgagttcg aatctcaccg
     3481 cttccgccaa tccttggggt caaaaaccaa ataaaaatga agtagcataa agcatcggca
     3541 tttttatttg gttttacttc attatcggaa acgtggcaga gaggctgaat gcagcggact
     3601 cgaaatccgc tgagggtgca aatcctccgt gggttcgaat cccaccgttt ccgccacaaa
     3661 acaaaaccgc cctgattcgg ggcggttctt ttttgttcaa gttgagacct ttgcaaaatt
     3721 cctttccttc ccgacagccg aaacccaaac acaggttttc agctatttcc gccccaaata
     3781 cctcctaatt ttaccc
//
LOCUS       DAWKQJ010000168         3744 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000168 DAWKQJ010000000
VERSION     DAWKQJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.168"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WJL20_001801"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WJL20_001801"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ7683915.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WJL20_001802"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WJL20_001802"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ7683916.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFITPADLARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1965..3206
                     /locus_tag="WJL20_001803"
     CDS             1965..3206
                     /locus_tag="WJL20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ7683917.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLIVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTGILIGGMVLTF
                     IWATGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            3264..>3744
                     /locus_tag="WJL20_001804"
     CDS             3264..>3744
                     /locus_tag="WJL20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7683918.1"
                     /translation="MELKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     "
BASE COUNT          887 a         1189 c          863 g          805 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatacc cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccaccgataa acgccttttt caacgcctca tccaacttat tgtccgctgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtcttg cgacaggacg ggcagcagcc gggtgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctccgccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttcgta tccgccacat ccaagcctgc
     1201 ctgacggcac atccgcgcca aatcggcagg cgtgatgaat tttttccaat cgtgcgtgcc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtggaaa aaaacaccat accgtccggt ttgaccagct tggcacaggc
     1381 acgaacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc agcgactgct ccgccatatc
     1561 gatgcctttg acaaacgccg cgccgcgccg cgccatactt tccgccaaaa ttccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgcg caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgccccact caaaaaccca agattatact atggaacgcg
     1861 atgccgtctg aaagcctttt cgggcggcgc ggcgcaaata ccttacaaac ccttacactt
     1921 tacggcataa tggcggcacg ctttttttgg cagaaagaca aaatatgccc aacaaaaccc
     1981 cttccctgtt cggcggcgcg atgattatcg ccggcacggt catcggcgcc ggtatgctcg
     2041 ccaacccgac cgccacctcc ggcgtatggt ttgccggctc gctgattgtg ctgctgtaca
     2101 cctggttttc tatgctctcc agcggcctga tgattttgga agtcaacacc cactatcccc
     2161 acggcgcaag tttcgacacg atggtcaaag acctgctcgg acgcggctgg aacatcatca
     2221 acggcatcgc cgtcgccttc gtcttatacc tgctcaccta cgcctacatc ttcgtcggcg
     2281 gcgatttgac cgccaaaggc atcggcaacg ccttgggcgg caatgtttcg ctcaccgtcg
     2341 gacaactcgt cttcttcggc atcctcgcct tttgcgtgtg ggcatccgca cgcttggtcg
     2401 accgcttcac cggcatcctc atcggcggca tggtattgac ctttatttgg gcaaccggcg
     2461 ggctgattgc cgatgccaaa ccgtccgtcc tgttcgacac ccaagcccct gccggcacaa
     2521 actactggat ttacgccgcc accgccctgc ccgtctgcct cgcctccttc ggcttccacg
     2581 gcaacgtctc cagcctgctc aaatacttca aaggcgacgc gcccaaagtg gctaaatcca
     2641 tttggacggg cacactgatt gcgctggtaa tttacgtcct ctggcaaacc gccatccaag
     2701 gcaacctgcc gcgcaacgag ttcgcccccg tgattgccgc cgaagggcaa gtctccgtcc
     2761 tgattgaaac cctgtccaaa ttcgcccaaa ccggcaatat ggacaaaata ttgtccctgt
     2821 tttcctatat ggcgatcgcc acctcgtttt taggcgtaac gctcggactc ttcgactaca
     2881 tcgccgacat cttcaaatgg aacgacagcg tgtccggccg caccaaaacc gccgcgctga
     2941 ccttcctgcc gcccctgatt tcctgcctgc tcttccccac cggcttcgtt accgccatcg
     3001 gctacgtcgg cctggcggca accgtctgga caggcatcat ccccgccatg ctgctctacc
     3061 gttcgcgcca aaaattcggc gcaggcaaaa cctataaagt ttacggcggc ttgtggctga
     3121 tggtttgggt cttccttttc ggcatcatca acatcgccgc acaggtattg agccaaatgg
     3181 aactcgtccc cgtatttaaa ggataaaggc aaaatgccgt ctgaagccct gttcagacag
     3241 ccttacgaca ttcggaaaat catatggaac tcaaaataga cattgcaacc aacaacttca
     3301 aacacggcgg cggcacggaa cgctacacat tggatttggt aaagggtctg aacagacaaa
     3361 acatcacacc ggccgtttat gcgacgaaat ttgatcacgg cattcctgaa tacgccatga
     3421 tcgaacccca tcttgtcgat caacaccgga cgctgaaaaa actacgctca ttcctctttt
     3481 caagtcggct cgctcaaacc agaaaaaaca gtgccgccaa actgattgcc tgccaccacg
     3541 ccgattacgc cgacctcctc atctgcggcg gcacacactt gggctacctg caccatatgg
     3601 cgcaaaaacc gaacctgctc gaccgcctcg ccatacgccg caaccgcagc aactacgcca
     3661 ccgccaaact gattatggcg cattcccata tgatgcggcg cgaactggtc ggactgtacg
     3721 gcgttccccc tgaaagaatc caag
//
LOCUS       DAWKQJ010000169         3641 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000169 DAWKQJ010000000
VERSION     DAWKQJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.169"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            275..622
                     /locus_tag="WJL20_001805"
     CDS             275..622
                     /locus_tag="WJL20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ7683919.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICI
                     PETLQTQMTHTRI"
     gene            619..3480
                     /locus_tag="WJL20_001806"
     CDS             619..3480
                     /locus_tag="WJL20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ7683920.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSAGGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          987 a          994 c          973 g          687 t
ORIGIN      
        1 atccactata actagggaaa ttcaaattaa gttagaatta tccctataag aaaaagccgt
       61 ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgaaagttc aaatttccat
      121 tttaaaacaa ttagtaaaat cgagtttatc ctagttgtcc aagacaaccc ctataataat
      181 ataattcaaa atataaaaac gggttacatc taaacattac ggaattttta ttccctcgcc
      241 tgaattctat tgtcagattc aaggagacct catcatgcga acgaccccaa ccttccctac
      301 aaaaactttc aaaccggctg ccatggcgtt agctgttgca acaacacttt ctgcctgctt
      361 aggcggcggc ggaggcggca cttctgcgcc cgacttcaat gcaggcggta ccggtatcgg
      421 cagcaacagc agggcaacaa cagcgaaatc agcagcagta tcttacgccg gtatcaagaa
      481 cgaaatgtgc aaagacagaa gcatgctctg tgccggtcgg gatgacgttg cggttacaga
      541 cagggatgcc aaaatcaatg cccccccccg aatctgcata ccggagactt tacaaaccca
      601 aatgacgcat acaagaattt gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      661 cgcggggtag aggtaggtat cgtcgacaca ggcgaatccg tcggcagcat atcctttccc
      721 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacta tacggcgtat
      781 atgcggaagg aagcgcctga agacggaggc ggtaaagaca ttgaagcttc tttcgacgat
      841 gaggccgtta tagagactga agcaaagccg acggatatcc gccacgtaaa agaaatcgga
      901 cacatcgatg tggtctccca tattattggc gggcgttccg tggacggcag acctgcaggc
      961 ggtattgcgc ccgatgcgac gctacacata atgaatacgc atgatggaac caagaacgaa
     1021 ataatgtctg cagccatccg caatgcatgg gtcaagctgg gcgaacgtgg cgtgcgcatc
     1081 gtcaataaca gttttggaac aacatcgagg gcaggcactg ccgaccattt ccaaatagcc
     1141 aattcggagg agcagtaccg ccaagcgttg ctcgcctatt ccggcggtga taaaacagac
     1201 gagggtatcc gcctgatgca acagagcgat tacggcaact tgtcctacca catccgtaat
     1261 aaaaacatgc ttttcatttt ttcggcaagc aatgacgcac aagctcagcc caacacactg
     1321 accctattgc cattttatga aaaagacgct caaaaaggca ttatcacagt tgcaggcgta
     1381 gaccgcagtg gagaaaagtt caatggctcc aaccattgcg gaattactgc catgtggtgc
     1441 ctatcggcac cctatgaagc aagcgtccgt ttcacccgta caaacccgat tcaaattgcc
     1501 ggaacatcct tttccgcacc catcgtaacc ggcacggcgg ctctgctgct gcagaaatac
     1561 ccgtggatga gcaacgacaa cctgcgtacc acgctgctga caacggctca ggacatcggt
     1621 gcagtcggcg tggacagcaa gttcggctgg ggactgctgg atgcgggtaa ggccatgaac
     1681 ggacccgcgt cctttccgtt cggcgacttt accgccgata cgaaaggtac atccgatatt
     1741 gcctactcct tccgtaacga catttcaggc acgggcggcc tgatcaaaaa aggcggcagc
     1801 caactgcaac tgcacggcaa caacacctat acgggcaaaa ccattatcga aggcggttcg
     1861 ctggtgttgt acggcaacaa caaatcggat atgcgcgtcg aaaccaaagg tgcgctgatt
     1921 tataacgggg cggcatccgg cggcagcctg aacagcgacg gcattgtcta tctggcagat
     1981 accgaccgat ccggcgcaaa cgaaaccgtg cacatcaaag gcgatctgca gctgggcggc
     2041 gaaggtacgc tgtacacacg tttgggcaaa ctgctgaaag tggacggtac ggcgatgacc
     2101 ggcggcaagc tgtacatgtc ggcaggcggt aagggggcag gctatctcaa ccgtaccgga
     2161 caacgtgttc ccttcctgag tgccgccaaa atcgggcggg attattcttt cttcacaaac
     2221 atcgaaaccg acggcggtct gctggcttcc ctcgacagcg tcgaaaaaac agcgggcagt
     2281 gaaggcgaca cgctgtccta ttatgtccgt cgcggcaatg cggcacggac tgcttcggca
     2341 gcggcacatt ccgcgcccgc cggtctgaaa cacgccgtag aacagggcgg cagcaatctg
     2401 gaaaacctga tggtcgaact ggatgcctcc gaatcatccg caacacccga gacggttgaa
     2461 actgcggccg ccgaccgcac agatatgccg ggcatccgcc cctacggcgc aactttccgc
     2521 gcagcggcag ccgtacagca tgcgaatgcc gccgacggtg tacgcatctt caacagtctc
     2581 gccgctaccg tctatgccga cagtaccgcc gcccatgccg atatgcaggg acgccggctg
     2641 aaagccgtat cggacgggtt ggaccacaac gctacgggtc tgcgcgtcat cgcgcaaacc
     2701 caacaggacg gtggaacgtg ggaacagggc ggtgttgaag gcaaaatgcg cggcagtacc
     2761 caaaccgtcg gcattgccgc gaaaaccggc gaaaatacga cagcagccgc cacactgggc
     2821 atgggacaca gcacatggag cgaaaacagt gcaaatgcaa aaaccgacag cattagtctg
     2881 tttgcaggca tacggcacga tgcgggcgat atcggctatc tcaaaggcct gttctcctac
     2941 ggacgctaca aaaacagcat cagccgcagc accggtgcgg acgaacatgc ggaaggcagc
     3001 gtcaacggca cgctgatgca gctgggcgca ctgggcggtg tcaacgttcc gtttgccgca
     3061 acgggagatt tgacggtcga aggcggtctg cgctacgacc tgctcaaaca ggatgcattc
     3121 gccgaaaaag gcagtgcttt gggctggagc ggcaacagca tcactgaagg cacactggtc
     3181 ggactcgcgg gtctgaagct gtcgcaaccc ttgagcgata aagccgtcct gtttgcaacg
     3241 gcgggcgtgg aacgcgacct gaacggacgc gactacacgg taacgggcgg ctttaccggc
     3301 gcgactgcag caaccggcaa gacgggggca cgcaatatgc cgcacacccg cctggttgcc
     3361 ggtctgggcg cggatgtcga attcggcaac ggctggaacg gcttggcacg ttacagctac
     3421 gccggttcca aacagtacgg caaccacagc ggacgagtcg gcgtaggcta ccggttctga
     3481 cggacaggaa gcagacagcc gcaaagatca cggtctttgc ggctgtttct tatgaaaaga
     3541 aaaccctatt ccaattgcct gcttctattg tttcaagact tcttccaaag attcggcatc
     3601 aatcagatgt atagcggatt aacaaaaatc aggacaaggc g
//
LOCUS       DAWKQJ010000170         3560 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000170 DAWKQJ010000000
VERSION     DAWKQJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.170"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..974)
                     /locus_tag="WJL20_001807"
     CDS             complement(120..974)
                     /locus_tag="WJL20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ7683921.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1103..1831)
                     /gene="rpsB"
                     /locus_tag="WJL20_001808"
     CDS             complement(1103..1831)
                     /gene="rpsB"
                     /locus_tag="WJL20_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ7683922.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2004..2237)
                     /locus_tag="WJL20_001809"
     CDS             complement(2004..2237)
                     /locus_tag="WJL20_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683923.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAEFEQQRAQRQKDSGTKP"
     gene            2315..2896
                     /locus_tag="WJL20_001810"
     CDS             2315..2896
                     /locus_tag="WJL20_001810"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ7683924.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            2957..3547
                     /locus_tag="WJL20_001811"
     CDS             2957..3547
                     /locus_tag="WJL20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7683925.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          777 a          823 c         1030 g          930 t
ORIGIN      
        1 ttttgttttc agacggcctt tatattcggt cgtcttaaaa taggtaccgc aaacggtttt
       61 ctcaaaaaaa agcaccctga ttcgtttgga atccaggtgc tttctttttc ataagtgcct
      121 tacactttgg cggctgcggc tacttcggct gcgtagtcga caacggcttt ctcgataccg
      181 tcgcctactt tgtagcgtac gaagctaacc acttcagtgc cgttttcttt agcgaattgg
      241 gcaacagttt ggtcagggtt catcacgaat gcttggccgt tcagagtgat ttcagccaag
      301 aatttacgga tacggccttc aaccatttta gcggcgatgt cggcaggttt gccggaagcg
      361 atggcttgtt cggtgtagat gtggcgttct ttttcaacgg tttcagcatc tacttcagct
      421 tcgcttacgc attgtggttt agcggcaacg atgtgcatac cgattttgcg tgctacgtct
      481 tcagagcctt tgtactcaac caatacgcct tcggtagcca atgcaccgtg gatgtaggca
      541 accagttggt tggcagtgtc gatcacttgg aagcgacgga cagacatgtt ctcgcccaat
      601 ttggcgatga tggctttgcg ttctgcttca accagttcgc tcagttcttc aacagaagcc
      661 ggttttttct cggcggcggt tttcgcaacg aagttggcaa attctacgaa gcccgcgtct
      721 ttagcaacga agtcggtttc gcagtttact tcaaccaatg cgccgacatt gccgttaatc
      781 gcgtaagcca atacgccttc ggcagcagta cggccagcca gtttaccggc tttcgcaccg
      841 gatttaatac gcaggatttc ttcggctttg tcgaagttgc cttcagcttc aaccaaggct
      901 tttttgcatt ccatcatgcc caggccggta gcggcgcgca gatcggcaac catttttgca
      961 gtaatttctg ccattttgaa tctcctagat ttttgggaaa tacggaaagg attttccgca
     1021 ttcgggaatc gtgtgccgcg aacggacgtt cagacggcat atttgagaaa aggggcataa
     1081 cgcccctctt cggtttgccg gattactcgg cagcggcttc ttgggcagcg gctacggttt
     1141 cttgcagcgc ttggtttttg ccttccaaaa ctgcgtcagc gatgccgcgg cagtacaggc
     1201 ggatggcttt ggcggagtcg tcgttgccgg ggatgacgta tttcacgccg tcagggctgt
     1261 tgttggtatc gactacggcg ataacgggga tgcccagttt ttcagcctcg accagagtac
     1321 ctttttggta gccggtatcg ataacgaaaa tcgcgtcagg caggcctttc atgtttttga
     1381 taccgcccaa agaacgttcc agtttttcaa cgtcgcgttg catttccaga atttcttttt
     1441 tgttgaaacc gccttcagca gcgttttcca gggcagcagt tttttcttcc aggcgtttga
     1501 tggattgctt aacggttttg tagttagtca gcataccacc caaccaacgg tgatcaacga
     1561 aaggcatacc ggcacgggtc gcttcttcgc ggatgatgtc gcgggcttgg cgtttggtac
     1621 ctacgaacaa tactgtacct ttgttggcaa ccagacgacg tacggcttct tgcgcctctt
     1681 ggaacatcgg cagggttttt tccaagttta cgatatggat tttgttgcgc gcaccgaaaa
     1741 tgtattgggc cattttcggg ttccagaaac gggtttggtg accgaagtga acaccggctt
     1801 caatcatctg acgcatagta atttgagaca ttttatttcc ttgaaagggt taagagcaca
     1861 cattcaatcg catagaactt gataggtcac gcctattgtc aagcaccctt cggcgaaagt
     1921 gggcataagt tttaaaaaat agtgtgtttc cgaaatggaa aactgacgga ttatagcgga
     1981 taagaggccg tctgaaaagg gattcagggt tttgtgccgc tgtctttttg ccgttgtgcg
     2041 cgctgctgtt cgaattcagc ctgttcctgc tgcctcatca ggtggttgcg gcggcggacg
     2101 gtaatggtga aaaccgtgaa cacggtgggc agcaccgccc aaaaaaccaa ataaatcaag
     2161 gcgcgcgcaa tactcggctg cgcggcggaa aacatcacgg caacaaaaag atagccgatg
     2221 gcgacgatgt gccacattgt gtgcgtcctg tttgaatgtt aacaaaatcg ttataatcgc
     2281 aacattctac cgcaatcctg aaaggccgtc tgaaatgagg aatgaggaaa aacgcgccct
     2341 gcgccgcgaa ttgcgcgggc ggcgttcgca aatggggcgg gacgtgcggg cggcggcaac
     2401 ggtaaaaatt aaccgcctgc tcaaacgtta tatcaagcac ggtcggaaaa tcggcgtgta
     2461 ttggccgatg ggcaaggaat tgcgtttgga cggctttgtc cgcgcggcgc aaaaacgcgg
     2521 cgcaaaactt tatctgcctt atatcgaacc gcgttcgcgg cggatgtggt ttacgccgta
     2581 tcctgaaagc ggaatggaac gggagcgcat acggggcagg gcgaggttga acgtgccgca
     2641 gtttgcaggg cgcaaaatcc gcgtgcacgg tttgtcggta ttgctcgttc cgcttgtcgg
     2701 catagaccgc gaaggctacc gtttggggca ggcaggcggc tattacgatg cgacgcttgc
     2761 agcgatgaaa taccgtttgc aggcaaaaac cgtgggcgtg ggctttgcct gccagttggt
     2821 ggacagactg tcggtcgagg cgcacgacct gccgctggac ggttttgtgt cggaggcggg
     2881 gatattgtgt ttttagccgt tcagacggca ttttgaacgg aaatgccgtc tgaaggtgga
     2941 atattgggag aaaaggatgt cggaaaagat aatactgccg gtcttgtcgt tgggtggcgt
     3001 acggctcgaa ccgttggatg tgcatcatga aacgggtttg cgcgaggcgg tttgcgatgg
     3061 ggaagtttgg aagctgggag taacgtccgc gccccatccg gatagggtgg cagactatat
     3121 cgggacagca ttggcaacgc gtttggcgtt tgcggttgtc gatgaagagg cgggcagggt
     3181 ggtcgggaca acggcgtatt atcattttga accgcagatt ccgcgtttgg atattggatt
     3241 cacatggtat gcggcatcgg cacggcggac acggataaac acctgttgca aaatcatgct
     3301 gttggattac gcatttgatg ttttggcttg ccgttgcgtg ggatggcgca cggatattct
     3361 gaaccttgcc tcgcaacgtg ccatcgaacg gctgggtgcg gaaaaagacg gcgtgctgcg
     3421 tatgcatatg ctccgtaaag acggcagtgt gcgcgatacg gtcgtgtaca gtatgctgag
     3481 ggaagattgg tgcaaaaaca gggaaatcct gaccggcagg ttggcagggt atggcgtgca
     3541 ggtgtgaggc agtattttca
//
LOCUS       DAWKQJ010000171         3534 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000171 DAWKQJ010000000
VERSION     DAWKQJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.171"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            458..3223
                     /locus_tag="WJL20_001812"
     CDS             458..3223
                     /locus_tag="WJL20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7683926.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYKPYKNYNNQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            3253..3495
                     /locus_tag="WJL20_001813"
     CDS             3253..3495
                     /locus_tag="WJL20_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683927.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVVYESSFPHRRESRHSTLRQFIGND"
BASE COUNT         1016 a          867 c          848 g          803 t
ORIGIN      
        1 tcataagttt cccgaaattc caacataacc gaaacctgac agtaaccgta gtaactgaac
       61 cgtcattccc acgaaagtgg gaatctagaa atgaaaagca acaggcattt atcggaaata
      121 actggaaccg aacagactag attcccgcct gcgcgggaat gacggctgca gatgcccgac
      181 ggtctttata gcggattaat aaaaatcagg acaaggcggc gagccacaga cagtacaaac
      241 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
      301 agcgagacaa cgctgtactg gtttttgtta atccactata aatatccaat tgaaatcttc
      361 agacggtata tcgaatttac actttttaat gtttatgccg cctaaaaaaa tgctaatata
      421 tttcttaatt gtctgactgt ttattgttga ggaaaatatg agatcttctt tccggttgaa
      481 gccgatttgt ttttatctta tgggtgttat gctatatcat catagttatg ccgaagatgc
      541 agggcgcgcg ggcagcgagg cgcagataca ggttttggaa gatgtgcacg tcaaggcgaa
      601 gcgcgtaccg aaagacaaaa aagtgtttac cgatgcgcgt gccgtatcga ctcgtcagga
      661 tatattcaaa tccagcgaaa acctcgacaa catcgtacgc agcatccccg gtgcgtttac
      721 acagcaagat aaaagctcgg gcattgtgtc tttgaatatt cgcggcgaca gcgggttcgg
      781 gcgggtcaat acgatggtgg acggtatcac gcagaccttt tattcaactt ctaccgatgc
      841 gggcagggca ggcggttcat ctcaattcgg tgcatctgtc gacagcaatt ttattgccgg
      901 actggatgtc gtcaaaggca gcttcagcgg ctcggcaggc atcaacagcc ttgccggttc
      961 ggcgaatctg cggactttag gcgtggatga cgtcgttcag ggcaataata cctacggcct
     1021 gctgctaaaa ggtctgaccg gcaccaattc aaccaaaggt aatgcgatgg cggcgatagg
     1081 tgcgcgcaaa tggctggaaa gcggagcatc tgtcggtgtg ctttacgggc acagcaggcg
     1141 cagcgtggcg caaaattacc gcgtgggcgg cggcgggcag cacatcggaa attttggcgc
     1201 ggaatatttg gaacggcgca agcagcgata ttttgtacaa gagggtgctt tgaaattcaa
     1261 ttccgacagc ggaaaatggg agcgggattt acaaaggcaa cagtggaaat acaagccgta
     1321 taaaaattac aacaaccaag aactacaaaa atacatcgaa gagcatgaca aaagctggcg
     1381 ggaaaacctg gcaccgcaat acgacattac ccccatcgat ccgtccagcc tgaagcagca
     1441 gtcggcaggc aatctgttta aattggaata cgacggcgta ttcaataaat acacggcgca
     1501 atttcgcgat ttaaacacca aaatcggcag ccgcaaaatc atcaaccgca attatcagtt
     1561 caattacggt ttgtctttga acccgtatac caacctcaat ctgaccgcag cctacaattc
     1621 gggcaggcag aaatatccga aagggtcgaa gtttacaggc tgggggcttt taaaggattt
     1681 tgaaacctac aacaacgcga aaatcctcga cctcaacaac accgccacct tccggctgcc
     1741 ccgcgaaacc gagttgcaaa ccactttggg cttcaattat ttccacaacg aatacggcaa
     1801 aaaccgcttt cctgaagaat tggggctgtt tttcgacggt cctgatcagg acaacgggct
     1861 ttattcctat ttggggcggt ttaagggcga taaagggctg ctgccccaaa aatcaaccat
     1921 tgtccaaccg gccggcagcc aatatttcaa cacgttctac ttcgatgccg cgctcaaaaa
     1981 agacatttac cgcttaaact acagcaccaa taccgtcggc taccgtttcg gcggcgaata
     2041 tacgggctat tacggctcgg atgacgaatt taagcgggca ttcggagaaa actcgccgac
     2101 atacaagaaa cattgcaacc ggagctgcgg gatttatgaa cccgtattga aaaaatacgg
     2161 caaaaagcgc gccaacaacc attcggtcag cattagtgcg gacttcggcg attatttcat
     2221 gccgttcgcc agctattcgc gcacacaccg tatgcccaac atccaagaaa tgtatttttc
     2281 ccaaatcggc gactccggcg ttcacaccgc cttaaaacca gagcgcgcaa acacttggca
     2341 atttggcttc aatacctata aaaaaggatt gttaaaacaa gatgatacat taggattaaa
     2401 actggtcggc taccgcagcc gcatcgacaa ctacatccac aacgtttacg ggaaatggtg
     2461 ggatttgaac ggggatattc cgagctgggt cagcagcacc gggcttgcct acaccatcca
     2521 acaccgcaat ttcaaagaca aagtgcacaa acacggtttt gagttggagc tgaattacga
     2581 ttatgggcgt tttttcacca acctttctta cgcctatcaa aaaagcacgc aaccgaccaa
     2641 cttcagcgat gcgagcgaat cgcccaacaa tgcgtccaaa gaagaccaac tcaaacaagg
     2701 ttatgggttg agcagggttt ccgccctgcc gcgagattac ggacgtttgg aagtcggtac
     2761 gcgctggttg ggcaacaaac tgactttggg cggcgcgatg cgctatttcg gcaagagcat
     2821 ccgcgcgacg gcagaagaac gctatatcga cggcaccaac gggggaaata ccagcaatgt
     2881 ccggcaactg ggcaagcgtt ccatcaaaca aaccgaaact cttgcccgcc agcctttgat
     2941 ttttgatttt tacgccgctt acgagccgaa gaaaaacctt attttccgcg ccgaagtcaa
     3001 aaatctgttc gacaggcgtt atatcgatcc gctcgatgcg ggcaatgatg cggcaacgca
     3061 gcgttattac agctcgttcg acccgaaaga caaggacgaa gacgtaacgt gtaatgctga
     3121 taaaacgttg tgcaacggca aatacggcgg cacaagcaaa agcgtattga ccaattttgc
     3181 acgcggacgc acctttttga tgacgatgag ctacaagttt taaaggcagc ccgcattttg
     3241 tagaaaaccg caatgccgtc tgaaagccct tcagacggca tttgtttccc caaacgcatc
     3301 atcctgccgc aagcctatgc caatccgttt tatcgcatcg gcaactcaaa gaaaaatcca
     3361 tttcattccc acgcagggaa gccggttttt gatttcggtt atttttggtt gtttcgggta
     3421 gtttatgagt cgtcattccc gcacaggcgg gaatctagac attcaacgct aaggcaattt
     3481 atcggaaatg actgaaactc aaaaaactgg attccctctt tcgtgggaat gacg
//
LOCUS       DAWKQJ010000172         3473 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000172 DAWKQJ010000000
VERSION     DAWKQJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.172"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..711
                     /locus_tag="WJL20_001814"
     CDS             43..711
                     /locus_tag="WJL20_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ7683928.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTANNKCDSGKKEQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGKTQPTADRVVWTFNQNGTFGYTN
                     DQHLTSQSSFSYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTV
                     CQYK"
     gene            741..1355
                     /gene="pilV"
                     /locus_tag="WJL20_001815"
     CDS             741..1355
                     /gene="pilV"
                     /locus_tag="WJL20_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ7683929.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFQVGV
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGAAPTLSDSGAFSRNCDDKA
                     NGDTLIKVLWVNDSAGDSDIARTNLEANGNNIVYTYQARVGGRE"
     gene            1352..2317
                     /locus_tag="WJL20_001816"
     CDS             1352..2317
                     /locus_tag="WJL20_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ7683930.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKNVVSDVAQKNRL
                     FSLKGSSANKLIPNGTDNKLIPITESLDIGYQGFTQRLNALVFQYGIDDLDASAETVV
                     VSSCSKIAKPGQKISTLQEAKSALQITNDDKQNGNITRQRYVVNAYAVGRIAGEEGLF
                     RFQLDDKGKWGNPQLLVKKINKMDIRYIYVSDCPEDDDAGKEETFKYTDKFDSAQNAV
                     TPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2296..2889
                     /locus_tag="WJL20_001817"
     CDS             2296..2889
                     /locus_tag="WJL20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ7683931.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGMKYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2894..3367
                     /locus_tag="WJL20_001818"
     CDS             2894..3367
                     /locus_tag="WJL20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ7683932.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQNIKSKLERFVSGYKMNPKIAEKYNVSVHFVNQEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDAGCEAFSNRKK"
BASE COUNT         1006 a          686 c          876 g          905 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggcca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggcgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtaagactc agccgaccgc tgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga acgaccagca tcttacaagc
      541 caatccagct tttcttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gttgaggttt gtcctagaaa cgataggcgt accgtatgcc aatataaatg attgttttag
      721 tcggtttggg aaataaaaaa atgaagaata atgattgctt ccgcctgaaa aacccccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg agggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatcataca ctatcagttg
     1021 tggatggcga ttttcaggtt ggtgtcataa aaactaagac gcagttggca gaggcacaat
     1081 tgaagagatt tagttatgag ctgaaaaatg ccttgccgga tgcggcagcc atccattacg
     1141 ccgtctgcaa ggattcgtcg ggtgctgcgc cgacattgtc cgacagcggt gctttttctc
     1201 gaaattgcga tgataaggca aacggggata ctttaattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatt gcccgtacga atcttgaggc gaacggcaac aatatcgtat
     1321 atacctatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acgtcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ctattcaaca ggatttgcgg aatgcggcaa cattgattgt
     1561 ccgcgatgca agaatggcgg ggagcttcgg ttgtttcaat atgtctgagc ataccgaaaa
     1621 gaatgttgtt tccgatgtgg cgcaaaaaaa ccgtcttttt tccttaaaag ggagcagtgc
     1681 aaataaactt atccccaacg gtacagataa taaacttatc cccataacgg aatctttaga
     1741 tatcggatat cagggtttta cccagcgcct taacgcattg gttttccaat acggtatcga
     1801 tgatcttgat gcgagtgctg agactgttgt agtcagcagc tgttccaaaa tagcaaaacc
     1861 gggtcagaaa atatctacct tgcaagaagc aaagagtgca ttacagatta ctaatgatga
     1921 taaacaaaat ggaaatatca cccgtcaaag gtatgtggtc aatgcctatg cggtcggcag
     1981 gattgccggt gaggaaggtt tgttccgctt ccaattggat gataagggca agtggggtaa
     2041 tcctcagttg ttggttaaaa agattaataa aatggatata cggtatattt atgtttccga
     2101 ttgtcctgaa gatgacgatg ccggcaaaga ggaaacattc aaatatacgg ataaattcga
     2161 cagcgcccaa aatgctgtta cgcccgccgg ggtggaggtt ttattgagta gcggtactga
     2221 taccaagatt gccgcttctt cagacaatca tatttatgct taccgtatcg atgcgacaat
     2281 acgcggggga aatgtatgcg caaacagaac gctttgacgg gaatcccgac ttctgacgga
     2341 cagagggggt tcgcactgtt tatcgtgctg atggtgatga tcgtcgtggc atttttggtt
     2401 gtaactgccg cgcagtctta caataccgag cagcggatca gtgccaacga atcagacagg
     2461 aaattggctt tgtctttggc cgaggcggct ttgcgggaag gcgaacttca ggttttggat
     2521 ttggaatatg atacggacag taaggttaca tttagcgaaa actgtggaaa aggtctgtgt
     2581 accgcagtga atgtgcggac aaataatgat aatgaagagg cttttgacaa tatcgtggtg
     2641 caaggcaagc ccaccgttga ggcggtgaag cgttcttgca ctgcaaattc taccaacctg
     2701 tgcattgaca ataaagggat gaaatataag aaaggcacgc aaagcgtcag caaaatgcca
     2761 cgttatatta tcgaatattt gggcgtgaag aacggagaaa atgtttatcg ggttactgcc
     2821 aaggcttggg gtaagaatgc caataccgtg gtcgtccttc aatcttatgt aagcaataat
     2881 gatgagtaat aaaatggaac aaaaagggtt tacattgatt gagatgatga tagtcgtcgc
     2941 gatactcggc atcatcagcg tcattgccat accttcttat caaagttata ttgaaaaagg
     3001 ctatcagtcc cagctttata cggagatggt cggtatcaac aatatttcca aacagtttat
     3061 tttgaaaaat cccctggacg ataatcagaa catcaagagc aaactggaaa gatttgtctc
     3121 aggctataag atgaatccga aaattgccga aaaatataat gtttcggtgc attttgtcaa
     3181 tcaggaaaaa ccaagggcat acagcttggt cggcgttcca aagacgggga cgggttatac
     3241 tttgtcggta tggatgaaca gcgtgggcga cggatacaaa tgccgtgatg ccgcttctgc
     3301 ccaagcccat ttggagacct tgtcctcaga tgccggctgt gaagccttct ctaatcgtaa
     3361 aaaataaggt tgttttgcca ataccgtctg aaaatcaatg ttcagacggt atttttatgg
     3421 gtatagtgga ttaacaaaaa tcgggacaag gcgacgaagc cgcagacagt acg
//
LOCUS       DAWKQJ010000173         3403 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000173 DAWKQJ010000000
VERSION     DAWKQJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.173"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..179
                     /locus_tag="WJL20_001819"
                     /pseudo
     CDS             96..179
                     /locus_tag="WJL20_001819"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            176..418
                     /locus_tag="WJL20_001820"
     CDS             176..418
                     /locus_tag="WJL20_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ7683933.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            496..741
                     /locus_tag="WJL20_001821"
     CDS             496..741
                     /locus_tag="WJL20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7683934.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            744..1133
                     /locus_tag="WJL20_001822"
     CDS             744..1133
                     /locus_tag="WJL20_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683935.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            1351..1623
                     /locus_tag="WJL20_001823"
     CDS             1351..1623
                     /locus_tag="WJL20_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683936.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
     gene            1745..2686
                     /gene="mafA"
                     /locus_tag="WJL20_001824"
     CDS             1745..2686
                     /gene="mafA"
                     /locus_tag="WJL20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7683937.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            2690..>3403
                     /gene="mafB"
                     /locus_tag="WJL20_001825"
     CDS             2690..>3403
                     /gene="mafB"
                     /locus_tag="WJL20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7683938.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPKGGNYPKPTGA
                     RDEYTYNVNGVARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT         1037 a          889 c          710 g          767 t
ORIGIN      
        1 actgtgattg aggaataagc ataattagct taatgaatag aatcagcgat atagattgga
       61 ctgcaaatcc acgcttatac gctgtgccat gattaacagg aagagagcaa cttggatggg
      121 ctagtaggga tggtaagcat ttaaatatat caattgatgg aaagattaca cacaaatgac
      181 taatttaaaa ttagatttct actctgaagt tattataaaa gattcttgcc ctaatgattt
      241 gttagaaaat ggtgagacta ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga
      301 agatggtata atctatggct atactatttt actttttgat ataaaatatt gtatatatat
      361 agataagaaa tatattatac cgactggaaa aaaattctct cgcgatgatt tttattagct
      421 aatttaagta atttaagcaa gttttttaga tactgaaact ggtaattatg tttatgtaag
      481 cataaggagt tcagattgaa ggattctaca gggcaaatga ggctggcaac caaggatttg
      541 gcggaagcca ttaaacgagg agaagtacgt agttctgctt ttacaacaaa gcaactaaag
      601 gcaattgaaa aaggcaaaga caaaatccct agctacactt ggcatcatca tcaagacact
      661 ggaaggatgc aattgattaa tgaagacttg catcatgata ccggccatat cggttggaga
      721 gcgatgagta aaggaaagta actatgtgga aaatcataaa agaggatagt gatgatttag
      781 gatttgcaat taaatgctta ttctctcagt ctattgattt aaatgaattc aagttatgga
      841 ttgaacaagt aatacgcgat atgcccatcg aggacatccc tttttatatt tttgacttgg
      901 cggattttga tgggggaatt gccgatattg acaatattgt aggttttgtt tcgagttaca
      961 gcctatcaaa atcgaaaaaa aatgccttga caggcattgc cttcttaagg gggatagatg
     1021 tctatgatcc gcctatttca aaagaaaaag cattaaaagc cttaaagaaa catcctgaaa
     1081 tttatcagaa gtttcagcat ttctttccgt ttgtagaact gcccccgctt taaacagtca
     1141 aaatgccgtc tgaaatgata ttcggctttc agacggcatt tttgacacaa agcaggtaac
     1201 caaaagagcg tttgacggca aaggaagata attatgtgaa aaattacgaa agagcaaggt
     1261 caagatttaa gttttgcaac atattgctta ttatcggcag caattaattt gcaagaattt
     1321 aaattatggt tggagaaggt tgtcttagat atgcctattg ataatatacc tttttatatt
     1381 tttgatttaa ttgatcttaa agaaggagta ggagatattt ataatatttt ggattttgtt
     1441 ccgaattcag acttatctaa agaccaagat gacgcattaa caggcattgc cttcttaagg
     1501 gggatagatg tctatgaccc tcccgtttca aaagaaaaag cattaaaagc cttaaagaaa
     1561 catcctgaaa tttatcagag gtttcagcat ttctttccgt ttgtagaact gcccccgctt
     1621 taaacagtca aaataccgtc tgaaagccat ttccgccgct cagacggcat tttcgcccct
     1681 tttgtttaca aacccttaaa atccatttac actcaaactc gttcaaaaca aaacaaaccc
     1741 tgccatgaaa accctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac
     1801 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
     1861 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
     1921 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
     1981 tatcgacgca ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag
     2041 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
     2101 ttccacatcg cttttgaacg cccccgccgc cgccctgact agaaacagcg gacgcaaagg
     2161 cgaacgctcc gccggactgt ccgtcaacgg cacgggcgac taccgcaacg aaaccctgct
     2221 cgccaacccc cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg
     2281 cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt
     2341 attcggcacc gtccgcagcc gcaccgaact gcacctctac aacgccgaaa cccttaaagc
     2401 ccaaaccaag ctcgaatatt tcgccgttga ccgcgacagc cggaaactgc tgattgcccc
     2461 taaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta
     2521 cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacatcac
     2581 cccctacggt gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa
     2641 ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct
     2701 gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc
     2761 cgccctcgcg gcggacttgg cgcaagatcc gttcattacc gataacgccc aacggcagca
     2821 ctacgaaccc ggaggcaaat accacctctt cggcgacccg cgcggcagcg tttccgaccg
     2881 caccggtcaa atcaacgtca tccaagacta tacccaccag atgggcaacc tgctcatcca
     2941 acaggcaaac atcaacggca caatcggcta ccacacccgc ttttccggac acggacacga
     3001 agaacacgcc cccttcgaca accacgccgc cgacagcgca agcaaagaac aaggcaacgt
     3061 tgacgaaggc tttaccgtgt accggctcaa ctgggaagga cacgaacatc atcccgccga
     3121 cggctatgac ggcccgaagg gcggcaatta ccccaaacct acgggagcac gcgacgaata
     3181 cacctataac gttaacggcg tagcccgcag catcaaactc aatccgaccg acacccgcga
     3241 cgtcagacaa cgcatatccg acaattacag caacctcggc agcaatttct ccgaccgcgc
     3301 cgatgaagcc aacagaaaaa tgttcgagca caatgccaag ctcgaccgct ggggcaacag
     3361 catggagttt atcaacggcg tcgccgccgg cgcgctcaac ccc
//
LOCUS       DAWKQJ010000174         3378 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000174 DAWKQJ010000000
VERSION     DAWKQJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.174"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(214..1278)
                     /locus_tag="WJL20_001826"
     CDS             complement(214..1278)
                     /locus_tag="WJL20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7683939.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTAGNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYHQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WJL20_001827"
     CDS             complement(1279..2037)
                     /locus_tag="WJL20_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7683940.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WJL20_001828"
     tRNA            complement(2112..2187)
                     /locus_tag="WJL20_001828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..>3378)
                     /locus_tag="WJL20_001829"
     CDS             complement(2241..>3378)
                     /locus_tag="WJL20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ7683941.1"
                     /translation="VDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVR
                     MMPHTTAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     SSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGSIEAAFAWGIADRQSNPEGKLPPQLW
                     DGQNDPDLPAINLTGGNSFWTTEKRIAASSSFAFGGSNCVLLIG"
BASE COUNT          769 a          822 c          899 g          888 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgac gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta ttttgaagga
      121 tgaattcggg gtgtttcttt ccaatgaacg aatataattt agtggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 tttcgtttca gggttcaaga cgaggcgttc gatttcgtcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggtgg taaaacgaag caagtttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaatcag gttttccaat cctttttctg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgccggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tcggtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattgtgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc gaataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ttcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc attgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagga tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 gtatttgttg aatttgttga aatattgttc ccagttgtcg tatgtgtaat ggtacataaa
     1561 atgcttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct tttttcggca tcagacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccattcaaa tgccgtctga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcctgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtaaggttga caaacttatt tcatccgatg agtaaaacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcc gttgtccaga agctgttgcc gccggtaagg
     2341 ttgatggcgg gaaggtcagg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgagcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttgcgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgagaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggtggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacgtgcc ggtcagcaac
     3121 aattcgccca catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atccgtccgt cggtaatgct ttcgtccccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccact
//
LOCUS       DAWKQJ010000175         3369 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000175 DAWKQJ010000000
VERSION     DAWKQJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.175"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(199..579)
                     /locus_tag="WJL20_001830"
     CDS             complement(199..579)
                     /locus_tag="WJL20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ7683942.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPSVQNQARPADF
                     REFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1055..3136
                     /locus_tag="WJL20_001831"
     CDS             1055..3136
                     /locus_tag="WJL20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ7683943.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQGEA"
     gene            3126..3320
                     /locus_tag="WJL20_001832"
     CDS             3126..3320
                     /locus_tag="WJL20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ7683944.1"
                     /translation="MKRKLAAWWETAKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          735 a         1000 c          856 g          778 t
ORIGIN      
        1 tcattggata acagccctga agaaatcgga gcaggtttaa agttggcatt gagccgctgc
       61 cgataggata aacccggaac gaaacgaaaa ataatccgcc acgcccgaaa accgccgaaa
      121 ccagccgaca gccaaaacaa atccgacaca aaaaaccgcc cgaaaaatcc ggacggcggt
      181 tcaaacaggc tgccccgttt agcgggcgcg gcaggaagtt tcgaccgaat tgccgtaggc
      241 atcggtaaag ccgaaaaagg cttcgccgcc tttctggtgc cactcggttg cgtttccgaa
      301 caaaccgtgt tcggcggtat agcgttcgcc ggatgcggca acgtcggaag agaggacggc
      361 acgcctgccg tccagccgca acgcgacttt gccgctgtcc aaatggcgga cgcgcacaga
      421 caaaccgttc tcgcatgaaa actcccgaaa atctgccggg cgggcttggt tttgaacgga
      481 cggcatatgc ccgcgtccgc cgtcatcata cgcctccggc acggcacagg ccgccaaaga
      541 caaaaccggt acggtcagcg cgaaaaacct gatattcata aaagctcccc aataaaaata
      601 agatatgaaa caaccgccct gattccaagc tgcggcaacg ccatactata aacggacgcg
      661 caaacacaca agcccgataa ccggaattta cccgcgatga atcaataatc cggattgcgc
      721 gccccttctt tacccgtctt ccggcgccgc cttttgcctg acgatgccgt ccaaacccgc
      781 ctgtccgccc gaaggaatgt aaattttttc caaattccaa gtaaaaaccg ctatcagtgt
      841 gctaatttgc gttaaaatcc tattcggcgt ttaacgtttt gtgcgcccgc atccctgcac
      901 tgtttgatgc gggcataagg cacaaatccc gacaagcgca ctgtttcata cttcgtcaat
      961 cattcagact ccggtttgtg cccgtgccgg caggcggtca tccgtttccc gccgttcagg
     1021 catattccga cagtgtgaga taaggattta ttcgatgaaa tcactcaaaa ccttcctcat
     1081 ttggggcata gtggtactgg tcggcttagc atcctttacc actctggctc tcagccgagg
     1141 cgaacaggtc agcgcggtat ggatggttac cgccgccata tccgtttact gcatcgccta
     1201 ccgtttttac agcctctaca tcgccaaccg cgtgatgcgg ctcgatccta accgcctgac
     1261 tccggcagaa cgccacaacg acggcttgga ctacgttccg acgcacaagg gcgtattgtt
     1321 cggacaccac tttgccgcga ttgccggcgc gggccctttg gtcggcccgg ttttggcggc
     1381 gcaaatgggt tacctgcccg gtactttgtg gattatcttc ggcgtggtgt ttgccggcgc
     1441 ggtacaggat atgatggtct tgttcgtctc tatgcgccgc gacggtaagt ctttgggcga
     1501 tattgtgaaa caggaactcg gcactgtccc cggcgtgatt gcctccatcg gtattttgat
     1561 gattatggtc atcattatgg cggtgttggc gttgattgtc gtaaaagcac tggttcacag
     1621 cccttggggt acgttcacca ttgccgccac tatgccgatt gccctgttta tgggtattta
     1681 cacgcgctat atccgtccgg gcaaaatcgg cgagatttcc atcgtcggct ttattttgct
     1741 gatgctggcg gtaatttacg gcgaagatgt ggcgaaaagt tccatcggac attggttcga
     1801 ccttgacggc atccagctca cttgggcgat tatgatttac ggctttgtcg cctccgtatt
     1861 gcccgtatgg ttgctgctca ctccgcgcga ctatctctcc accttcctga aaatcggtac
     1921 gattgcggcc ttggctttgg gcatcgtcat cgtcaatccc actttgcaaa tgcctgccgt
     1981 aacccacttt atcgacggtt cgggtccggt attctcaggc gcattgttcc cgttcctctt
     2041 catcaccatc gcctgcggtg cggtttcagg cttccacgcg ctgatttctt ccggcaccac
     2101 gccgaaaatg ctggaaaacg aaacccacgt ccgtatgatt ggttacggcg gtatgttgat
     2161 ggaaagtttc gtagccatta tggcgcttgc cgccgccgca tcgcttgatc ccggcgtgta
     2221 cttcgccatg aacagccctg ccgccctgat cggtacggat gccaacaccg ccgccgaagt
     2281 gattaccacc aagctgcaat tccctgtcga tgccgcaacg ctgttgcaca ccgctaaaga
     2341 agttggagaa aacaccatcc tgtcccgtgc cggcggtgcg cccaccctcg cagtcggtat
     2401 ggcgcacatt atgagccgcc tgattccggg cgaggcgatg atggcgttct ggtatcactt
     2461 cgccctgttg tttgaagcct tgttcatcct gaccgccgtc gatgccggta cgcgcgtcgc
     2521 acgttttatg attcaagact tgggcagcat cttctacaaa cctttcggca acaccgactc
     2581 catccccgcc aacctgattg cgaccttctt cgccgtggca ttgtggggct actttctcta
     2641 caccggcgta accgacccgc tgggcggcat caactcgctc tggcctttgt tcggcatcgc
     2701 caaccaaatg ctggcgggcg tggccttgat tatgtgtgcc gtggtgctga ttaagatgaa
     2761 acgcgaccgt tatgtttggg tggtactcgt tcccgccgtc ggcgtactgt tcgtaacctg
     2821 ctacgccggc ctgcaaaaac tgttccacag cgatccgcgc atcagcttcc ttgcccacgc
     2881 cggcaaatac agcgacgcat tggctaaaaa cgaaatcctt gcgcctgcca aagacatcgg
     2941 cgaaatggcg caaatcatct tcaacgacaa gattaatgcc ggtctgacca tcctcttctt
     3001 gtcggttgtc gtgattgtcg ccgcgtacgg tttgcgtacc gccctcaaag cacgcaaagt
     3061 cggctggcca accgccaaag aaatccctgc agtgtaccgt gacggcaaac aaccggaggc
     3121 acaaggtgaa gcgtaaactc gctgcttggt gggaaaccgc caaactgacc gccaacctca
     3181 tggcaggcgt gcccgattat gaaaactacg ttgcacagca gcgcaaacat aatcccaacg
     3241 cccctgtgat gaccaagctg cagtttcaag actattgccg caaacgccgc tgcggcgcaa
     3301 acggcggacg ctgctgttaa gcctgcttga aacaaattcc gtctgaacgc cgcttcagac
     3361 ggaattttt
//
LOCUS       DAWKQJ010000176         3283 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000176 DAWKQJ010000000
VERSION     DAWKQJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.176"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..2222)
                     /locus_tag="WJL20_001833"
     CDS             complement(63..2222)
                     /locus_tag="WJL20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7683945.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAGNANVALDTITVKGDRQGSKI
                     RTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNAY
                     SDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLDK
                     NWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKTV
                     PYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDRQ
                     APAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNHT
                     QITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEEKNGQKVDKP
                     MEQQMKDRADEATVHAYKLSNPTKTDTGAYIEAIHEINGFTLTGGLRYDRFKVKTHDG
                     KTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKA
                     ERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGY
                     ELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNP
                     NLEIGWRGRYVQKAVGSILAAGQKDRKGNLENVVRQGFGVNDVFANWKPLGKDTLNVN
                     LSVNNVFNTFYYPHSQRWNNTLPGVGRDVRLGVNYKF"
BASE COUNT          726 a          754 c          933 g          870 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatatgcg
       61 ttttagaact tgtagttcac gcccaggcgc acatcacggc ccacgcccgg cagggtattg
      121 ttccagcgtt ggctgtgcgg atagtagaac gtgttgaaca cgttgttgac tgaaagattg
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga atacatcgtt cacaccgaaa
      241 ccttggcgta caacgttttc caagttgcct ttgcggtctt tttgacctgc cgccaatatc
      301 gaacccacgg ctttttgaac ataacggccg cgccagccga tttctagatt cgggtttttg
      361 aagcggtagg caagggaggc tgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gcttacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtagccgg cgttgacggc ttcacggacg gcggcagaga cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ttgccagaag tagctgccgt tggcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg ctttcgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttgcc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcgt gggtttttac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagggtaaag ccgttaattt cgtgaatggc ttcgatatac
      961 gcgccggtat cggttttggt cgggttggaa agtttgtagg cgtgaacagt ggcttcatct
     1021 gcacggtctt tcatttgttg ttccatcggt ttatcgactt tttgaccgtt tttctcttct
     1081 gtcgtcggga tggagaattt cgaattcaaa aacgcttgcg gtttgatttc ctgatggcgg
     1141 tagttgatac cgtatttcag cagggtttgt tcggcaaggc ggctgtcgaa gttgaaattc
     1201 ataccccgag tggtgatttg ggtatggttg gggcctttta cattgcctgc gtagccggtg
     1261 ccgctgtcat cggcggaata gcgttctttt tccaacacat aggcgttggc atccagtttt
     1321 tcgacaaagc ccaggttttt acccgtgtac gccaaattgg tgttggattg tgtggtttcg
     1381 cggtaagcag gggcttggcg gtcaatattt atccgtgaac ttttgtcgcc gacggtaaat
     1441 tcttcacgca cagtgcggat gcctcggtgc tggtctttca tatggctcaa tacgatgcgg
     1501 tggtcgccgt cgccgaagct tgttccgatt ttggcgaggt agctgcgttt gtccagtgcg
     1561 ctgtacggta cggttttgcc gccgttgaca ttgcggaaac ctttgccggc ttcgtaatct
     1621 ttttcatcgt tgcggttgta agagaacaaa ccgtcgaagt tgccctcttt tccgaatacg
     1681 cttgcgccgt agcttacgcc ttcgttgctg gcaaagccgc tgttgaggcg cacgccccag
     1741 tttttatcca agcctttgag caggtcttgg gcatcgacgg ttttggcgat aatcgcgccg
     1801 ttggttgcgc cgataccggc agaggcagaa cccgcgcctt tttgtacgga aacgacttta
     1861 accaaagcgg gatcgacaat aaatctgcct tggtggtaaa ggatttggct gtcggaatag
     1921 gcgttgtcca ccttgatgtc gacagagttc tgacccatgc cgcgcagcgt caggaattgg
     1981 gacgtgccgt tgccgccgcc gaaatcgatg gagggctctt cttttaagag ttcgcgcata
     2041 tcggttgcgg tgctttcgtc tttttgttga agcgtaacga tgttggtacg gattttgctg
     2101 ccttggcggt cgccttttac ggtaatggta tccaatgcga cattggcgtt gcctgccgcg
     2161 tgggcgaaac ctgccgcaag tgtgagcgag agcaggctga gacggaacaa tggggtattc
     2221 attcaatcgt cctcttgagt gtatgaaagg aaggaaatcc aaaccgttaa gatttggcag
     2281 gataagaaaa ataattaata attattttta tttatattaa cagggggggg gatttttgca
     2341 aagctgatta ttgtttttat ttgcattgtg ttgttttttg ttgacagatt ttgtcggaaa
     2401 tgtatagtgg actaacaaaa atcaggataa ggcgacgaag ccgcagacag tacagatagt
     2461 acggcaaggc gaggcaacgc tgtactggtt tttgttagtc cactataaaa aacggcggaa
     2521 atatagtgga ttaacaaatg cgggaatgac gaaaagcaac ctttccgcgt cattcccgcg
     2581 aaagcgggaa tctagaaccc ctaacgcggc aggaatctat cggaaatgac tgaaaccgaa
     2641 cagactggat tcccgcctgc gcgggaatga cgaagcctgt gcgggaatga cgaggcctgc
     2701 gcgggaatga cgaaaagcaa cctttccgcg tccttcccgc gaaagcggga atctagaacc
     2761 cctaacgcgg caggaatcta tcggaaaaaa ccgaaaccga acagactgga ttcccgcctg
     2821 cgcgggaatg acgaagcctg cgcgggaatg acgaaaagca acctttccgc gtccttcccg
     2881 caaaagtggg aatctaggaa ctcaaagctg caagaattta tcagaaacaa ctggaactca
     2941 aaaaaactag attcccgcct gcgcgggaat gacgaagggt taaagaaatg atgagaaacg
     3001 gcggtaattt atcgggaata accgaaaacg aacggaaatc aggacaaggc ggcgaagccg
     3061 cagacagtac ggataggacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
     3121 tctctttgag ctaaggcgag gcaacgacgt actggttttt gttcatccac tataacagca
     3181 accctgtcgc cgtcattccc gcgaaagcgg gaatccagtc cgttcagttt cggtcatttc
     3241 cgataaattc ctgctgcttt tcatttctag attcccactt ttg
//
LOCUS       DAWKQJ010000177         3230 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000177 DAWKQJ010000000
VERSION     DAWKQJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.177"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            124..2142
                     /gene="uvrB"
                     /locus_tag="WJL20_001834"
     CDS             124..2142
                     /gene="uvrB"
                     /locus_tag="WJL20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ7683946.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNTIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKENLLF"
     gene            2153..2944
                     /locus_tag="WJL20_001835"
     CDS             2153..2944
                     /locus_tag="WJL20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683947.1"
                     /translation="MIKMKDIYESICCKSIRKIMYISDSFVAISGTGFIIKFQEKFYF
                     CCLYHCMSNEGKFEKNKLFGLSILYKTYLEIDPKTISESNVANYVLFPNPEQITKEFC
                     SSKIDDDLMLDEEFIPIEIIGENELIPCGYLDFDIEYWSNYLFSEKKPIENEILLVCG
                     FPFSDNEIDYTDDGEIKKVSHQVHIYAGKCKKDDIGFFVEKEDKNVSFDCNGVSGGIV
                     MRMIDGHSEWVGIAVRANNDIIRFIPYYLVAKSIIQNILNRNQQA"
     gene            3063..3134
                     /locus_tag="WJL20_001836"
                     /pseudo
     CDS             3063..3134
                     /locus_tag="WJL20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          946 a          827 c          697 g          760 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcattcgata ccccgattcc cccttcaaac tccaccaacc cttcccgccc
      181 gccggcgacc agcccaccgc cattgccggc ctgctcgaag ggctttcaga cggccttgcc
      241 tatcaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctc
      361 tatgccgaaa tgcgcgagtt tttccccgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc agcccgaagc ctatgtgccc agccgcgact tgttcatcga aaaagacagc
      481 gcgatcaatg aacacatcga acaaatgcgc ctttccgcca ccaaaaacct gatgacgcgc
      541 gacgacgtga ttatcgtcgc caccgtgtcc gccatttacg gtatcggcga cccgactgag
      601 tatcaacaaa tggtgttgtc cgtcaaagaa ggcgacacca tcgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg cggcagcttc
      721 cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgt
      781 atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttatccaac gcgtcggccg ctacaccgtc ttcccgtcca gccactacgt taccccgcgc
      901 gacaccgtat tgcgcgcctg tgactccatc aaagaagaat tgcgcgaacg cattgatttc
      961 tttaccaaag aaaaccgtcc cgtcgaacaa caacgcatcg aacagcgcac ccgtttcgac
     1021 ctcgaaatgc tctacgaaat gggcttctgt aaaggaatcg aaaactactc ccgccacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc aagcaacacc
     1141 atcatgttca tcgacgaaag ccacgttacc gttacccaaa tcggcggcat gtacaaaggc
     1201 gacgcctcgc gcaaacaaaa cctcgtagat tacggcttcc gcctgccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaaccgt cttcgtttcc
     1321 gccactcccg ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgt
     1381 cccacagggc tggtcgaccc ccaaatcatc atccgccccg tcgccaccca agttgatgac
     1441 ttaatgagcg aaatcaacga ccgtattcaa aaaggcgaac gtgtgctcgt taccaccctc
     1501 accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat caaagtacgc
     1561 tacctgcaca gcgacatcga caccgtagag cgcgttgaaa tcattagaga tttgcggctc
     1621 ggcctgtttg acgtactcgt cggcatcaac ctcttgcgcg aaggcctcga catccccgaa
     1681 gtctccctcg tcgccatcct cgacgccgac aaagaaggct tcctgcgctc ccaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcacgc aacgtgaacg gcgtcgccat cctgtacgcc
     1801 gataaaatca ccgactccat gaaagccgcc atcgacgaaa ccgaacgccg ccgcgaaaaa
     1861 cagattaaat tcaacgaaga acacggcatc gtaccgcagc agattaaaaa acaggtaaaa
     1921 gacattatcg acggcgtgta ccacgaagaa gacggcggaa aaggccgtct gaaaggtaaa
     1981 aacaaggtta aagtgggcga gattcacaac gaagaagatg cgattaaaga aatcgccaaa
     2041 ctcgaaaaag ccatgcagca ggcggctagg gatttgcagt ttgaagaggc ggctgtgttg
     2101 cgggatagga ttcgggggat taaggagaat ttattatttt aggatgtaaa aaatgattaa
     2161 gatgaaagat atttatgagt caatttgttg taaatctatt agaaagataa tgtatatatc
     2221 agatagtttt gtagctattt caggaacggg gtttattatt aaatttcagg aaaaatttta
     2281 tttttgctgt ttatatcatt gcatgtctaa tgaaggaaaa tttgaaaaaa ataaattgtt
     2341 tgggttaagt attttatata aaacatatct tgaaatagat cccaaaacta tatctgaatc
     2401 taatgtagct aattatgtac tttttcctaa tcctgagcaa attacaaaag agttttgtag
     2461 ttcaaaaatt gatgatgatt taatgcttga tgaggaattt atccctattg agattattgg
     2521 agaaaatgaa cttataccat gtgggtattt ggattttgat atcgaatatt ggagtaatta
     2581 tttgttttca gaaaaaaaac caatagaaaa tgaaattctt ttagtatgcg ggttcccttt
     2641 ttcagataat gaaatagatt atactgatga tggtgagatt aaaaaagtat cgcatcaagt
     2701 gcatatatat gctggtaaat gtaaaaaaga cgatataggc ttttttgttg aaaaagaaga
     2761 taaaaatgtt tcatttgatt gtaatggtgt tagtggtgga attgtgatga gaatgataga
     2821 tggacacagt gagtgggttg gaattgctgt tagggcaaat aatgacatca ttcgttttat
     2881 tccttattat ctcgtagcga aatcaataat ccagaatatc ttaaatagaa atcaacaagc
     2941 gtaggtcgga tacttgtatc cgacaaaacc ttcaacattc ccgtcgttgc aatccattgc
     3001 agctatgtcc gaaatgtcgg attcgaaaat ccgacctaca aaatcattcc gagcataaca
     3061 ctatgaaata acgtcgtttt taccgcaatg acggcactta cttttttacg gttgcaacca
     3121 ataaacggca gcaagcgtag ggtgggcact tgttgcccac gcgtttatct ccgaaaggca
     3181 gcaactgtag gtcggatact tgtatccaac aaacctttaa cattctcgtt
//
LOCUS       DAWKQJ010000178         3191 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000178 DAWKQJ010000000
VERSION     DAWKQJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.178"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1227
                     /locus_tag="WJL20_001837"
     CDS             52..1227
                     /locus_tag="WJL20_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003682122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7683948.1"
                     /translation="MYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVR
                     VSLLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQ
                     NNTILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQARED
                     RNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGD
                     KTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVIS
                     ADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKA
                     QAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            1272..1562
                     /locus_tag="WJL20_001838"
     CDS             1272..1562
                     /locus_tag="WJL20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683949.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1567..1764
                     /locus_tag="WJL20_001839"
     CDS             1567..1764
                     /locus_tag="WJL20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683950.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1772..2056
                     /locus_tag="WJL20_001840"
     CDS             1772..2056
                     /locus_tag="WJL20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683951.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            2063..2341
                     /locus_tag="WJL20_001841"
     CDS             2063..2341
                     /locus_tag="WJL20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683952.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            2469..2786
                     /locus_tag="WJL20_001842"
     CDS             2469..2786
                     /locus_tag="WJL20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7683953.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            2770..3165
                     /locus_tag="WJL20_001843"
     CDS             2770..3165
                     /locus_tag="WJL20_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683954.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFAKVSG"
BASE COUNT          973 a          707 c          715 g          796 t
ORIGIN      
        1 acagaaaccg aaacggcagg cagggttcag gaagtcttcg aatgttacga aatgtacata
       61 acggacggta aaggaaacct gttaggcgtt cctcttcggc gcggtgtatc agattcggct
      121 ttcattgatc aaattagctt ttcatttcat gaaaaaacct ttttcgataa atacggcgtt
      181 cgtgtaagtc ttttggaaga cgaagatttc attcgcgccg cgtccatgct cgccgaagaa
      241 gttttcggtt tcggtatcta caaagagtcc aaaggctcgg gcggtcgttt ttatgagcgc
      301 tgttggttga tgggttcgga agacgtccta tacggtcgcg tccattttgg cggacaaaac
      361 aataccatcc ttttcgaact gaccggcacc ggttgcggcg tcgcaaaaga aggctgggaa
      421 tcacgacttt tcgcattcct gactaatgca atccgcccaa aaatcacacg cgttgacatc
      481 gcaaaagact ttttcaacgg cgaatacagc ccgaaccaag cccgtgaaga ccgaaataaa
      541 ggtatgttta cctgtcatca cgtcaaacca aaaggcgaat gtttggggtc agattgggaa
      601 gaagacgatg aagccaaaat gaccaaaggc aagacctatg gtatcggctc ccgtgaatcg
      661 tccaaatatg tccgcgtcta tgaaaaaggc aagcagttgg gcgataaaac aagcacatgg
      721 acgcgctttg aaattgaatt caaagcaaaa gacatcgtta tccctttcga agttttgcag
      781 aatccgggcg aatatttcgg cggcgcatat ccgatttgcg aacgattcgc ccaaaaggca
      841 acgcgcatac acgcggttaa ggaagataag gtcatttcag ccgaccgcta ccttgaatgg
      901 gtaaaaaaac agttcggacg tgcggcaaac ggtctgaaat tcatttttcc cgaattggac
      961 aaagccaaac tgtttgaact gattgagccg agtcatcaca agctgcccaa gtctttggct
     1021 cccgaagcct acgactgcgc ctttttgaaa gctcaagcca ttcatgaaca gcccgcattc
     1081 aaaccgtaca aagaccctta ctatatgtac gaatattacg agaatcttga aaaacagctt
     1141 gaacagcaaa aacacgtcaa caatgaagaa agctataaca acttcattta cgacaaattc
     1201 gcaagactac cgatttcatg ggcttaaagt gtctgcccga aagacgttta atcacacaag
     1261 gaaaccaaaa aatgaacatc caacttcaag gccacatcgt cggcgttaaa aaattcaacg
     1321 gacaaatcga aggcaagagc ttcgactatt gccgcctgat tgtcgccaca cccttagaca
     1381 gctcccaagg caacgcattg ggcagctcta ctactgaata cgatttcggc ggctctgcca
     1441 atttcgagca gttccgaaac gcccaatttc ccatcgaagc aaacctgaac gtagaaatcg
     1501 ttactacggg caaaacccaa aaactgaaag tcatcggttt tcaactcgtg aagaaaggct
     1561 gattgaatgc agaaagtcta tgttgtccag tccgtatcaa caggggactt tctgtatctc
     1621 tctcctgaaa cgggcgacat cggacatacc aaattaatca ccaatgccga ttatttctac
     1681 gacttcgaag aagcgattaa cgcaggtttg gaagaaatcg gcaaccaata cgaatttgtc
     1741 gtattcggat ttttgaaaga ctgattttcg gatgttcggc ggtcgtctga aaaacgctcc
     1801 atccattacc gccaaacact ttttgaagga aaatatcatg aaatttatta acacctgccg
     1861 taaatacggc gcaaaactgg ctgttgtaac agctgctccc ctggctttgg ccgcacatgc
     1921 aaatgcaacg ttgcccgata cagcaaaaaa cgctttggaa gccgcaaaag ccgacggtat
     1981 ggaagccggt tggattgtcg ttggcgtttt cgccgcgctt tttgtctttt ccatcgttaa
     2041 gagagtgatg aagtaagacg gcatgtacta ccaagtcgga aataaatgtc ttgagaagca
     2101 ccaggctgaa aacctttatt tcagcttggt agtaccaaga atcaaagaaa acggacagat
     2161 tgtcaggccg gaatataacg gcagcctgtg gaagatgtcg gacggtcagc cgctaaggct
     2221 tttattggcg gaatgcagtc cgaaagacaa cctgcaaagc ggtcttgaaa caggctggat
     2281 agtattcggc atcctcgcgt ccgtttactt tgtttccctg ctgaaaaagg ttttgaaatg
     2341 atggattttt atttttatct cggcgtttcc gtacccgtat taatcggggc ggttctgttt
     2401 aagaattgag cgcatgaagt tatggtgtca aaatcaggct ttcaaaacaa tcattgaaag
     2461 gcagaaccat gaacaagccg tttatcactc aggcgcagtt ggcactttat aaatatcagc
     2521 cgtcaagcaa gtattttggg caatcgatgg cggttatagc gcaatctgaa tttgttgaat
     2581 ttgcgaagat taataagtct gaaaatgtta ttgattgttt ctcttttttc tggaatagaa
     2641 gaattaaaca tgatatttgg ctaatctcat tttctgataa ttcagaaatg gtaattaaag
     2701 aatccctgaa agatggtcat aaaatataca aatttgaatt ttgcgaaatt gtcgataatt
     2761 gcaattttga tgatgtattc gtttgaagcg aatgcaaatg cagtaaaaat atctgaaact
     2821 gtttcagttg ataccggaca aggtgcgaaa attcataagt ttgtacctaa aaatagtaaa
     2881 acttattcat ctgatttaat aaaaacggta gatttaacac acatccctac gggcgcaaaa
     2941 gcccgaatca acgccaaaat aaccgccagc atttcccgcg ccggcgtatt ggcggggggc
     3001 ggcaaacttg cccgcttagg cgcgaaattc agcacaaggg cggttcccta tgtcggaaca
     3061 gcccttttag cccacgacgt atacgaaact ttcaaagaag acatacaggc acgaggctac
     3121 caatacgaca ccgaaaccga caaatttgca aaggtctcag gctaagtgcg cctgttgccg
     3181 cctaaaaggt a
//
LOCUS       DAWKQJ010000179         3169 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000179 DAWKQJ010000000
VERSION     DAWKQJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.179"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            72..497
                     /locus_tag="WJL20_001844"
                     /pseudo
     CDS             72..497
                     /locus_tag="WJL20_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            503..1732
                     /locus_tag="WJL20_001845"
                     /pseudo
     CDS             503..1732
                     /locus_tag="WJL20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1790..2410
                     /locus_tag="WJL20_001846"
     CDS             1790..2410
                     /locus_tag="WJL20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7683955.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2499..2630
                     /locus_tag="WJL20_001847"
                     /pseudo
     CDS             2499..2630
                     /locus_tag="WJL20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2627..3121
                     /locus_tag="WJL20_001848"
     CDS             2627..3121
                     /locus_tag="WJL20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ7683956.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
BASE COUNT          801 a          834 c          818 g          716 t
ORIGIN      
        1 actatattat cccgtatgcg gattggtttt aagatttgta aatttaattt gcatcaaaaa
       61 atcgccgata gatgattcat ataatatcaa tattaaagag tatcggtata tcggggatag
      121 tcatgtcctg tttttcaatc aaacgtatgt ccgcgtttcg ggcgcggata acagtgtttt
      181 ttaccgcctt tgtctttttg acggcggcac tgcccgctta tgcggagcgt ctgcctgatt
      241 ttctggcgaa aatacagcct tcggaaattt ttccgggtgc ggaccgttac ggcaagccgg
      301 aaggcaagcc tatggttgcc cgcgtttaca aaggcgatga gcagttgggc ttggtctata
      361 tcacgaccga tgcggtcaat acgcgcggtt attcgagcaa accgattgat acgctgatgg
      421 cgttggcaaa cgacggaacg atagccggag cgaaattgat tgatcaccat gagtcgatta
      481 tgctgatcgg tatcccgcat ttgcccgcgc ccgggcgggc gatacgctca aactggcttc
      541 cggcgtatat caaaccaaac tttatatcga caaaccgatt acgattgaag gacctgccga
      601 ccgttccgca accatcgaag gggacaggag cggtcgtacg atagctgtgc atgcgccgga
      661 cgtaacgctc cgcaacctga ccgttacacg ttccggtatg agcctgccag caatggatgc
      721 cggtatttat ctcgaagaaa ccgccccacg cgccctgatt gaacacaaca atatttttga
      781 taattcggtc ggcgtatatc tgcatggttc tgccgaagcg atggtgcgcg agaataaaat
      841 cgtcggcgac gcgactttgc gcgtgaacga gcgcggcaac ggcgttaccg tttggaacgc
      901 acccggtgcg caggtcgtcg gcaacgacat ttccaaaggg cgggacggca ttttttccaa
      961 caccagcacg cacaacacct ataaaaacaa ccgcttcagc gatttgcgtt tcgccgtcca
     1021 ctatatgtac accaacgaca gcgaagtcag cggcaatatt tccgtgggca acaatatggg
     1081 ctatgtgctg atgttttccg aacggctcaa agtattcgac aatattgccg tcggtagtcg
     1141 cgactagggc atcatgctca actatgtcaa ctattccgat attcacgaca atattatcaa
     1201 caaagcgggc aagtgcgttt ttgcctacaa tgccaactac gataaactgt ccgccaatca
     1261 ttttgaaaac tgccaaatcg gcatacactt taccgccgcc atcgaaggca cgtctctgca
     1321 cgacaattcc tttatcaaca acgaaagcca ggtcaaatac gtcagcacgc gctttctcga
     1381 ctggagcgag ggcggacacg gcaactattg gagcgacaac agtgcgttcg atttgaacgg
     1441 cgacggcttc ggcgacagcg cgtaccggcc caacggcatc atcgaccaaa tcatctagcg
     1501 cgcgcccgta ttgcgcctct tgatgaacag tctcgcaatc agcatcgtca aatgggcgca
     1561 ggcgcagttt cccgccgttc tgcccggcgg cgtggtggac agcaaaccgc tgatgaagcc
     1621 ttatgtcccc aaaattcaaa cccgctatca ggcaatgaag gacgagttgc tcaaagaagc
     1681 cgaaacacgg cagtcggaat ggggcagggc ggaaaacggt tctttgaact agtctgcttc
     1741 agacggcatc cggatccaaa tgccgtctga aaacacaaaa ggaacaacca tgaccacaca
     1801 tcatgtcgaa ttgaggaatg tcaccaaacg gttcggggcg caaaaagccg tcaaccaagt
     1861 cgatttggtt ttgaaggcag gagaaagcgt cgggcttgcc ggatacaacg gcgcgggcaa
     1921 gtccaccatt atgaagctga tactcgggct gattaccccg accgaaggcg aagtgatgct
     1981 tttgggcgaa cgtaccggca gcaaagcggg ggcgcggctt cgcagccaaa tcggctacct
     2041 gcccgaaact gttgcgttgc acccttcgct gaccggcatc gaaacgctgg atttttatgc
     2101 caaacttaaa aaacagccgc tcacgcagaa tcgggggctg ctcgagcgcg tcggcatttc
     2161 acaggcggca caccgccgcg tcggcactta ttctaaaggg atgcgccaac gccttgcctt
     2221 ggcacaagcc ctgctgggcg agcccaaagt cctgctgttt gacgaaccga caaccggcct
     2281 cgaccctgca tcaaggcaaa tgttttacga agtcgtgcgc gaactcaacg ggcgcggcgc
     2341 gaccgtattg ctcagtaccc acgcccttgc cgagttggac ggacacgccg accgcattat
     2401 cgtggattaa atttaaacca gtacggcgtt acctcgcctt accgtactat ttgtactgtc
     2461 tgcggcttcg ttgccttgtc ctgatttttg ttaatccact atatgcgggt atggcgggtt
     2521 tgagcggaca aatcagcctg accgtccccg ttttgctgac cgctcaggtt ttatgggtta
     2581 tcattccgct tgttttggca gccggaattt ttagaaagcg acaaatatga aaaaaaccct
     2641 gttggcaatt gttgccgttt ccgccttaag tgcctgccgg caggcggaag aggggccgcc
     2701 gcctttaccc cggcagatta gcgaccgttc ggtcggacac tattgcagta tgaacctgac
     2761 cgaacacaac ggccccaaag cccagatttt cttgaacggc aaacctgacc agccagtttg
     2821 gttctccacc atcaagcaga tgttcggcta taccaagctg cccgaagaac ccaaaggtat
     2881 acgcgtgatt tacgttaccg atatgggtaa tgttaccgat tggacaaatc ccaatgccga
     2941 cacgaagtgg atggatgcga aaaaagcctt ttatgtcatt gacagcggtt ttatcggcgg
     3001 tatgggtgcg gaagacgcac tgccgttcgg caacaaagag caggctgaga aatttgcaaa
     3061 ggataaaggc ggtaaggttg tcagtttcga cgatatgcct gatacctata ttttcaaata
     3121 aatccaaaca aaaatgccgt ctgaacgttg aagccaaggt tcagacggc
//
LOCUS       DAWKQJ010000180         3165 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000180 DAWKQJ010000000
VERSION     DAWKQJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.180"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(52..1152)
                     /locus_tag="WJL20_001849"
     CDS             complement(52..1152)
                     /locus_tag="WJL20_001849"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ7683957.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
     gene            1477..3051
                     /locus_tag="WJL20_001850"
     CDS             1477..3051
                     /locus_tag="WJL20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ7683958.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          660 a          808 c          916 g          781 t
ORIGIN      
        1 ggaaaaaatg ccgtctgagc ggcggaacgg gctttcagac ggcatttgcg ctcaataata
       61 atatcccgcg cccagaatac acggtttgga tgcgccggtt gctttgtgcg aactaccggg
      121 aatgcgatta atccaacacg ccgccaacca cgcaaatgcg gctgcttcca cccattgcgg
      181 atcgagcttc agttcggcgg tgctgcgtaa ggaaacgcgt gtgccgaaat attccgccaa
      241 atccgccatt aaaacaggat tgcggatgcc gccgccgcaa atgtacattt gacgggcatc
      301 tgccgctgcg tgtgagacgg cgtcgcaaac ggtttgcgcg gtaaaacggg aaagcgtccg
      361 caatacgtcg tatcggtttt caccgccgtc aaggtaggtt tcgagccaat ttagggcaaa
      421 cagttcgcgc cccgtgcttt tagggtgggg ttgtgcgaaa tacgggtggg cgagcagcct
      481 gtcgagcagt tgcggcaata tgttgccttg tgccgccttt gcaccgtttt tgtcgtaagg
      541 aagctgccag tgtgcctgcg tccacgcgtc catcagcata ttgcccggcc ctgtgtcgaa
      601 gccgaaggcg ggtgcgtcgg aggggagtac gctgatgttg gcaatcccgc cgatgttcag
      661 taccacgcgt gtttctttgt catcgcggaa cagggctttg tgaaaggcgg ggacgagcgg
      721 cgcaccttgt ccgccggccg caaggtcgcg gctgcggaag tcgccgacgg taaaaatctg
      781 agtcagttcc gccagcagcg gcaaatcggc aagctgtatg ctgtaaccgt gttccggcgc
      841 gtgtcggacg gtttgcccgt ggcagccgag ggcggtaatg tcggacggcg cgaggttttg
      901 actgcacagc agttcggcgg cggtttgcgc gtacaggcgg ctgagttctt gcgacaaaat
      961 cctgctgcgg tgcagttcgt ccgcccccgt gtcctgcaaa tccagcaatt ggcggcgcag
     1021 cctgtcggga tagggggtaa aggcgtgccc ttccgcgccc agccatttgc cgccgtccat
     1081 ccgtaccagc acggcatccg ccccatccat actggttccc gacatgatgc cgatgtaaag
     1141 ctgtgtttcc atcatcactc ccaaactggt gcaaaacgcc attttaacgt gtattgacgc
     1201 tcgtataccg atttgccgcc gcagtgtaaa taaagtgtaa ataaatgttt caagacggat
     1261 ggaaaaatat tataatgcgc ccgcaacatc cagtagtaga agtgtcatac aaaccgtttc
     1321 cggcagcagt tttgcattcg gtcaggtttg ggggtattcg gatgcggttc agaaggaagg
     1381 atgcgtctgc catatcccga aacggcagtt cgaccggagg cagcagtaca gtgtcggcaa
     1441 cactcatgat ttccaccaca ttaaaggaag attgccatgg ctcaaatcca aatgagcgca
     1501 aatgttaaaa ccatcaacgc cgtctttgcc gccatgctgg taggtacagt cggctatttt
     1561 atttattggg gcttgggtta tacccattac aattacgccg ccttattcat tattgccacg
     1621 atgttcggcg tgtttatggc gttcaacatc ggcggcaacg atgttgccaa ttctttcggc
     1681 accagcgtcg gtgcgggtac gctgaccatc ccgcaggctt tgctgattgc ggcggtattt
     1741 gaggtcagcg gcgcggtcat cgcgggcggc gaggtaacca ataccatacg caaaggcatc
     1801 gtcgatttga agggtgttga tttcgaaccc atacagtttg tgtttattat gatgtccgcg
     1861 cttttggcgg cggcgttgtg gctgttgttt gcctcgaaaa aagggcttcc ggtatctacc
     1921 acccattcca ttatcggcgg cattgtcggc agcgcggtat gtatggcggt aatgaacgat
     1981 gccgcatcgg gcgatttgat acgttggggc aagctgggcg gtattggtgt ttcttgggta
     2041 ttgtcgcccg tgttgggcgg cgcggtgtcc tatttcctgt tttcgcgcgt caagaaaaac
     2101 gtcttagatt acaacgcttg ggcggaaggc acgctcaagg gcatcaagca ggaaaaaaag
     2161 gcctataaag aacggcaccg cctgtttttc gagggtttgt ccgaagccga aaaagtcgag
     2221 tacgccacca aaatggcgca cgacgcgcaa atttacgacg aacccgaatt cgatccgcaa
     2281 gagctgcaat cggagtatta ccgcggtctt tatgcgttcg acaaccgtaa aaacaatgtc
     2341 gattcctaca aagcactgca ttcttggatt ccctttatcg cttcgttcgg cgcgatgatg
     2401 atttccgcta tgctgatttt caagggcttg aaaaacctgc atttggggat gagcaacgtc
     2461 aacagcttcc tgaccatctt tatgataggc gcggcggtgt ggatggggac gtttgtgttt
     2521 gccaaaagcc tcaagcgtaa agacttgggc aaatcgacct ttcagatgtt ttcatggatg
     2581 caggtcttta ccgcctgcgg cttcgcattc agccacgggg cgaacgatat cgccaacgcc
     2641 atcggtccgt ttgccgcgat tatggatgtt ttgcgtacca acagcgttgc cgcgcaaaat
     2701 gtcgtccccc cgattgcgat gctgactttc ggcatcgcgc tgattgtcgg tttgtggttt
     2761 gtcggtaaag aggtgattaa aaccgtcggt acgagtttgg cggaaatgca ccccgcctcg
     2821 ggttttaccg ccgaactgtc cgccgcatcc gtcgtgatgg gcgcgtcgct gatggggctg
     2881 cccgtgtcca gtacgcatat cttggtcggc gcggtactcg gcatcggtct ggtcaaccgc
     2941 aatgccaact ggaaactgat gaagcccatc ggtttggcgt gggtcattac cctgcctgcc
     3001 gccgccgtat tgtcggttgt ctgctacttg gttttacagg cagtattctg attgtaaaat
     3061 actgatgccg tctgaacccg tgttcagacg gcattttgtt gatggaatgt gcgggcttgt
     3121 gccttatgca caatctgttc tgtcgggata tgccgtttgg tatag
//
LOCUS       DAWKQJ010000181         3124 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000181 DAWKQJ010000000
VERSION     DAWKQJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.181"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..982)
                     /locus_tag="WJL20_001851"
     CDS             complement(131..982)
                     /locus_tag="WJL20_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7683959.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTVAKYRESFEIPAA
                     HKRKTAE"
     gene            1330..2844
                     /gene="katA"
                     /locus_tag="WJL20_001852"
     CDS             1330..2844
                     /gene="katA"
                     /locus_tag="WJL20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7683960.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDVQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
BASE COUNT          746 a          884 c          811 g          683 t
ORIGIN      
        1 ctttgcgttt ctagattccc tctttcgcgg gaatgacggg atgcaggttt ccgcgcggac
       61 ggattcggca ttcccgcttt cgcagaaata ccggcttttg gtttgttgtc tttataagat
      121 tattcggcaa ttattctgcg gttttgcgtt tgtgtgctgc cggaatctca aaggattctc
      181 tgtatttggc aacggtgcgg cgggaaacct ccatgccgcg gaaagccagc aggttggcga
      241 gtgcctcgtc agaatacggc gtgggcgtgg acgaggcaaa accggcggcg ggaaagggat
      301 tgagcgaagc aatgaggtcg agtgcggctt cgagtgtgcc gctgtcggtt tggggcaggc
      361 gtttttttat tcgcgcgagg gtttggctgc ggttgccgtc aatgctgtcg agggcgtttc
      421 ggacgatatg cagggcggag ggtttggcag cacattcgcc cgatctttct atctgcagta
      481 tcagcgattc gttcaaatcg gcggcggcca cgcctgccgg gtcgaatttt ttcaatgcgg
      541 tcagggcttg tttcagcatt gcttcatcca acatccactc caagggcgta tggtcgagga
      601 tgtcttcgat gctgtcggtc agataacctt gctcgtcaag gaaatcgatg aggatgtgga
      661 cacaggcgga ttcttggtcg gaaagcgggt gttcgcatac ttgcgcgtgc aggtattgct
      721 tgaaatcctg ctcgccggcg atgttggaca gcatatcttc gccttcgtct ccgccgattt
      781 gacgggcagg cgcagtgtaa tggctgaact cggcatcgga aaattcatcc gtgtctttgc
      841 gttcgagcag ggggttgtcc gacagccagt tttcgacctc gcgttcaagt tcgatacccg
      901 acatctgcaa tacgcgcaaa gattgttgca gccgctggtt gagctgctgg gtctgtttga
      961 gctttattcc gagtaaggtc atgataatgt ggggaaaatt gttattttca gcctgtcggc
     1021 gcaaaaaatg ccgcaaagcg tcattgcatt ataaatggtt ttaatgagcg ggttcggatt
     1081 ccgttcgata acaaaaaaca aacgaaaatc aagaaccgat tgcttataat aatattaaat
     1141 cgatttcata gttttaatag cgaaaatctt ggcgtatagt cgcatccata gtttttacaa
     1201 aagggaaata aaatgtcgat tcaagaaatt tattgcaatc aagaaaccgg ttacgaatac
     1261 gctttccgcc aaatcgtact gtaagccgtt tacccccatt tgaccaacct gagaaaagga
     1321 acaagagcga tgactacctc caaatgccct gtaacccatc tgaccatgaa caacggcgcg
     1381 cctgttgccg acaatcaaaa cagcctgacc gccggtcctc gcggccctct gctggcgcag
     1441 gatttgtggc tgaatgaaaa actcgccgac ttcgtgcgcg aagtcatccc cgaacgccgt
     1501 atgcacgcca aaggttcggg cgcgttcggc acgtttaccg taacgcacga catcaccaaa
     1561 tacacccgcg ccaaaatctt cagcgaagtc ggcaaaaaaa ccgagatgtt tgcccgtttc
     1621 accaccgtgg caggcgaacg cggcgcagcc gatgcggaac gcgacatccg cggttttgcc
     1681 ttgaaatttt ataccgaaga aggcaactgg gatgtggtcg gcaacaacac gcccgtgttc
     1741 ttcctgcgcg acccgcgtaa gttccccgac ctgaacaaag ccgtcaaacg cgacccgcgc
     1801 accaatatgc gctctgccac aaacaactgg gacttctgga cgctgctgcc cgaagcactg
     1861 caccaagtga ccatcgtgat gagcgaccgc ggcatccccg ccggctaccg ccatatgcac
     1921 ggcttcggtt cgcataccta cagcttctgg aacgaagcag gcgagcgttt ttgggtgaaa
     1981 ttccatttcc gcacccaaca aggcattaaa aacctgacca acgaagaagc cgccaaaatc
     2041 atcgccgacg accgcgaaag ccatcagcgc gacttatacg aagccatcga acgcggcgag
     2101 tttccgaaat ggacgatgta catccaagtc atgcctgaag cagacgcgga aaaagtacct
     2161 tatcatccgt ttgacttgac caaagtttgg ccgaaaaaag actatccgct gattgaagtg
     2221 ggcgaattcg agttgaaccg caatcccgaa aacttcttcg ccgatgtgga acaatccgcc
     2281 ttcgcaccga gcaacctcgt tcccggtatc ggcgcgagcc cagataaaat gttgcaagcc
     2341 cgtttgttca attacgccga cgcgcaacgc taccgcttgg gcgtgaactt ccgccaaatc
     2401 cctgtcaacc gtccgcgttg ccctgttcac agcaaccagc gcgacggaca aggacgcgcc
     2461 gacggcaact acggcagcct gccgcactac gaacccaata gcttcggcca atggcagcaa
     2521 caacccgact tcgccgaacc gcctttgaaa atcaacggcg acgcggcgca ctgggactac
     2581 cgccaagacg atgacgacta tttcagccaa ccgcgcgccc tgttcaacct gatgaacgac
     2641 gtgcagaaac aggcattgtt cggcaacacc gccgccgcaa tgggcgacgc gcccgacttc
     2701 atcaaatacc gccatatccg caactgctac cgttgcgatc cggcatacgg cgaaggcgtg
     2761 gcaaaagccc tcggactgac tgtcgaagac gcacaagccg cccgcgcgac cgaccccgca
     2821 ctcggccagc ccggtttgct gtaagggggc attatgtgga tggaaattga agaaatcctg
     2881 tcccgcgccg tccgctaccg gaaataaccg ggcataaaaa tgccgtctga aacgtcgttt
     2941 gaccgcttca gacggcattt ccccatcccg cccccgccgt ttcagtgggc gttttttatt
     3001 aaacgcaaaa tatcccgtca ttcccgcccc acagcccact tttccgccgt cattcccgca
     3061 ccatccccac cggttcaaac cggcacgaaa acttttccgc gtcattcccg cgaaagcggg
     3121 aatc
//
LOCUS       DAWKQJ010000182         3100 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000182 DAWKQJ010000000
VERSION     DAWKQJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.182"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            271..894
                     /locus_tag="WJL20_001853"
     CDS             271..894
                     /locus_tag="WJL20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7683961.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WJL20_001854"
     CDS             1005..2387
                     /locus_tag="WJL20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7683962.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2390..2914
                     /locus_tag="WJL20_001855"
     CDS             2390..2914
                     /locus_tag="WJL20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ7683963.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          626 a          839 c          935 g          700 t
ORIGIN      
        1 ctgctgccgc ccgaaaagcg tgatgcgctg atggtgctgc tgcataatcc ggacgacgga
       61 taaccggtga aaacggaaaa tgcccgggtg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtaaaatcaa ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgtgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgaacgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagataacc
     1021 gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgtcgg gctgattgtg tttgcagcgg gcagcttcct tgccgccgcc gccgatacgc
     1321 tgcccatgct ggtcgccgca cgcgccatac agggtgcggg agcggtcagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcgatg gcgatgatcg
     1441 gtttgagtat cggtctgacg ttttcggtca gcctcgtcgt tgcccctgtg attgccgacg
     1501 cggtgggcgt acgcggcttg tttctgctga ccggtatttt gaccgttgcc agtatcggcg
     1561 tggtggcgtg gatgactccc gacccggagg tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctc gatttcggca
     1681 ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccctcgacc attacgggct
     1801 tggtggtgat ggttccactg attatcgtcg gagagacgcg caacaagctc aaacaggttt
     1861 ttgttttggg catcgtctgc atcgcggcgg cacagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgtggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagcatattg atgctgctgt
     2161 ggctggtgat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcaggtc aatcgggacg gattgcaacg cgccctgttg caactcgaag
     2281 gcgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcattacagg agtttaaaaa tgtcattgaa
     2401 caaagttatc cttatcggcc gcctcggacg cgatcccgaa gtgcgctata tgcccaacgg
     2461 cgaggcggtg tgtaatttca gcgtcgccac cagcgaaact tggaacgacc gcaacggcca
     2521 acgtgtagag cgtactgagt ggcacaacat cacgatgtac cgcaaactgg cggaaattgc
     2581 cgggcaatac ctcaaaaaag gcgggctggt ttatctggaa ggcagaatcc aaagccgcaa
     2641 ataccaaggc aaagacggca tcgaacgcac cgcttacgat attgtcgcca acgaaatgaa
     2701 aatgttgggc gggcgcaatg aaaacagcgg cggtgcgcct tacgaggaag gttacggtca
     2761 gagtcaggag gcttaccaac gccccgcaca gcaaagccgg cagcccgcct ccgacgcgcc
     2821 gtcccatccc caagaagcac cagccgcgcc gcgccgccaa cccgtgcctg ccgccgcccc
     2881 ggtcgaggac attgacgacg atatcccgtt ctgaatttta cggccggaca tcctcgcgga
     2941 gggaaatcat aaaggacgga gaaaccttaa accttacggg gcagggtttc tcttttttgc
     3001 atttgggccg gcttgttgcc gccctttgcg ttgggcggaa cgttgcgatt gcgccgttcg
     3061 ggcgttttga ggcagtgatg cctgccgcac gtccccgctg
//
LOCUS       DAWKQJ010000183         2998 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000183 DAWKQJ010000000
VERSION     DAWKQJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.183"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(38..763)
                     /locus_tag="WJL20_001856"
     CDS             complement(38..763)
                     /locus_tag="WJL20_001856"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ7683964.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVVFPDRLHIPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="WJL20_001857"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="WJL20_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ7683965.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="WJL20_001858"
     CDS             1486..1863
                     /locus_tag="WJL20_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7683966.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="WJL20_001859"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="WJL20_001859"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ7683967.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2679..2924)
                     /locus_tag="WJL20_001860"
     CDS             complement(2679..2924)
                     /locus_tag="WJL20_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683968.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
BASE COUNT          755 a          988 c          676 g          579 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacaccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgtaat gcgaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt caccgctttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaatatgcag cctgtcggga aacaccaccg cacggggccc gaccgcatac caaaaaaacc
      601 acccgtcccg ggaaaaccac ggaaacacgc cgttccgata cgcctcaagc agccgccccg
      661 catccaaatc gccgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catgacagtt ctccaaaaac
      781 acaggcggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcttccgcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tgtggtcatg gtggtcgcct ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatgat ggcgttgcaa aatgcccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gtgccgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaaccgcg cgccgttgcc
     1621 gccctccgcc ccggcatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagaac gcagcaatct gaccgtctat tttgaaaacg gcgtactcgt ccgcaccgaa
     1801 ggcgacgtcc tgcaaaacgc tgccgaagcc ctcaaagacc gccaaaacac agacaaacca
     1861 taaggaacac acatgacacc gctcaaaatc gccatcgccg gcgcaaacgg ccgtatggga
     1921 cgcgtattgg ttgaagccgt caacaaccat cccgacaccg tcctttccgg tgcgcttgaa
     1981 cactcaggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacgccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agcccaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ctatccacac tgccgccgaa
     2221 caaacaggca tcgttttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacatc
     2281 ctcgacaccg tcgcccgcgt gttaaacgaa ggctacgaca tcgaaatcat tgaaggccac
     2341 caccgccaca aagtcgatgc ccccagcggc accgcgttac gcatgggcga agtcatcgcc
     2401 ggcgcgctcg gcagagacct caaacaatgc gccgtttacg gccgcgaagg ccacaccggt
     2461 ccgcgcgatc cgtcgaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgag cgcgtggaaa tcacccacaa agccggcagc
     2581 cgcatgacct ttgccgccgg cgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggacgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatgtttc
     2761 aacacacggg acgatacata aagcgtcgcc ctatgtgtcg tcctgatttg gaagggatta
     2821 cgcccctccc aaataaagtc tgattctacc gccccgaagg acagatgtcc gagtggcggg
     2881 gtttcaaccg aaaaggaaat acgataaagt tgcttgcgcg gcatgacaag ttacggctcg
     2941 tttaattaaa actctcctga tctaaaaatt ctaactctat ttcccggcaa actatatc
//
LOCUS       DAWKQJ010000184         2980 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000184 DAWKQJ010000000
VERSION     DAWKQJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.184"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..691
                     /locus_tag="WJL20_001861"
     CDS             <1..691
                     /locus_tag="WJL20_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7683969.1"
                     /translation="TADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETV
                     YIEISDGIGNNQTLISNRIHPFYSDSKWIKAEDLKAGSRLLSESGKTQTVRNIVVKPT
                     PLKAYNLTVADWHTYFVKSSQAETEGVWVHNDCPPYKRPNNATTKAQRESVQGKPCVE
                     YGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQYSKE
                     QKRKLSNGNKK"
     gene            675..1028
                     /locus_tag="WJL20_001862"
     CDS             675..1028
                     /locus_tag="WJL20_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683970.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1018..1341
                     /locus_tag="WJL20_001863"
     CDS             1018..1341
                     /locus_tag="WJL20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ7683971.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1400..1627
                     /locus_tag="WJL20_001864"
     CDS             1400..1627
                     /locus_tag="WJL20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683972.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1755..2297
                     /locus_tag="WJL20_001865"
     CDS             1755..2297
                     /locus_tag="WJL20_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7683973.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRSEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            2378..2914
                     /locus_tag="WJL20_001866"
     CDS             2378..2914
                     /locus_tag="WJL20_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7683974.1"
                     /translation="MDYNQTVLSHLQNFWKHHDIKGFTWALGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDRFQLGKTVN
                     IGRPWVEQSSFQHFLISLPYPYGQELEYMDNVRFFWLLPITQPERLFLNTHSVEDLET
                     RFDELGIDYLDINRASVI"
BASE COUNT          972 a          540 c          605 g          863 t
ORIGIN      
        1 aacggcagac ggctacaaag ccattgccca tattcaagcc ggcgaccgcg tcttcgccaa
       61 agatgaaacc agtggaaaaa ctggatacaa acctgttacc gcccgatacg gcaatccgta
      121 ccaggaaacc gtttacattg aaatttcaga cggcatcggc aacaaccaaa ccctgatttc
      181 caaccgcatc catccgtttt attcggacag caaatggatt aaagcggaag atttgaaagc
      241 gggaagcagg ctgttatccg aaagcggcaa aacccaaacc gtccgcaaca tcgttgtcaa
      301 accaacaccg ctcaaagcct acaatctgac cgttgccgac tggcatacct acttcgtcaa
      361 aagcagtcag gcggaaacgg aaggggtttg ggttcataat gattgtccgc cctataagag
      421 accaaataac gcaacgacaa aagcacaacg agaaagtgta caaggaaaac cctgcgttga
      481 atacggtaaa ttagccgaaa aaatgattgc cgaccataaa aaaccattag ttgtcgagta
      541 ttatgaaaca gggacaattg acaaatctaa gatgagagca attgaatcag tacaaccgca
      601 atgtccgact tgttctgcaa aacaaggggg gagattaagc caatattcaa aagaacagaa
      661 aaggaaactg agtaatggga ataaaaaatg aacaaataat aatttgtaaa aaatataata
      721 cagaaatata tcctatttcc gatgtttcaa aaatcggtgt tgctgaaaat gtcaaacagg
      781 caaacctgta tccgattaac gggttaaggc accgtcccaa aggtgatact aacggttggt
      841 atatctgggc cggtgaaaac ttctcctatg ataaaaattt tttcctgccc ttgcatactt
      901 tccatttgca aatatggcga cccgaaatca tccccttttt gacacttccg cccggttacc
      961 gttttttaat tggggaaaac ggttatgaag acgtatggtt tgatgagttg ctattaaatg
     1021 ataattgacg aaaaatctac ttcagatgta ctgaaacaat atcaagaact agggtttgat
     1081 ttgacaaaac ctatgataat agagtttttt ataggaggtt ctcaaaagaa tctgcaatca
     1141 atagaaagcc ggctcatttc ttatcggcaa gattttcaaa tcagtattga acaagatgaa
     1201 ttcggagaaa tgtggacttg ctattgttcc gtcaatatcg tcccttccct tgaaaacatt
     1261 ttagctattg aaactacgtt atttggtatt gcacaaaaaa atgattgtaa ttatgaaggt
     1321 tttggttcgt atgggaacta acaaaaagtg taggttgggt tgaaaaaacc atattcccat
     1381 taaagattag ggtgtgtcta tggacataaa attcaatact ttgggggtta tcctgaatgg
     1441 ggtcaatcct gaagaaaaat tcataaaaat tattgatgat caggaaaata ccgggggatt
     1501 tctgatactg ttatcttcaa atgacaaatt ctcatcattt gacagttacg atgactgggt
     1561 cgaaaactta gaaatattaa aagaatacct gcaagaatct cattggataa taaagtgggt
     1621 cggatagtcg gcaaacgtag tccgtctgaa acgcttttac ttttcagacg gactttctat
     1681 caatgggaac aaggcgtttt gtctcaatct ggtctatgat tatttcgata cagtgatggg
     1741 aagctaagaa aaatatgatt aatcatattg aaaaatatac aggcactatt tctcatggct
     1801 cgaaaagtga ttcgggaagt cagtacaaat taaatatcgc agctattccc tcttccccta
     1861 acagggattt aaaaacttat attactttag ggttaagcaa acatgatctg aattataaaa
     1921 gcagatctga aatacttttt gtatgttctt taaaatatga tgaaaatcag atttttccat
     1981 ttttaagatg gttggcagaa accatcattg agaataaaaa aatccttctt cgggggcagg
     2041 ttgtttattt gcccagaagc attgttgatt caacaaaaat ggatgcactg tatgtttccg
     2101 ccccatttta tttcgatgat gattttcagg tctgttatgg cgaacactac aatatcgttt
     2161 tccctttgct tgtccccttg tataaacagg aagcagaatt ggtggaaaag aaaggttgga
     2221 atgcttttga gcagttcttg ttggataatg aagttggcaa cctttcggat atgaatcgga
     2281 aaccgtttac ttggtaaata ccttcttcaa acggtcaatg ccgtctgaaa aaccttgttt
     2341 caggtttcag gcgacctttg aaaacaggct aaaaagcatg gactataacc aaactgtttt
     2401 atctcatttg caaaattttt ggaaacatca tgatattaaa ggatttactt gggctttagg
     2461 aaggattgtt gaagaacttc ccgatttcca agttttccaa gtgattccta atcatgaaga
     2521 tgagccttgg gtttatgtca gctctggaat aggtcaattt ttagggcagg agttttttat
     2581 catcagccca tttgaaacac ctgaacatat tgagaccttg gcaatgctgg catctgcaag
     2641 tatgcattat cccgatcgat ttcaattagg gaaaacggtc aatatcggca gaccgtgggt
     2701 tgagcaatca tccttccagc attttctgat ttctctgcct tatccttatg ggcaagaatt
     2761 ggaatatatg gataatgtcc gatttttctg gttattgcca attacccagc cagaaagact
     2821 tttcttgaat actcattcag tagaagactt ggaaacgaga tttgatgagc taggtattga
     2881 ttatttggat ataaatcgtg caagcgttat ttgatattta tgttgtttga aatctttcag
     2941 caagcgtagg ttggattgga aatccaacaa tcaaaatgcc
//
LOCUS       DAWKQJ010000185         2930 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000185 DAWKQJ010000000
VERSION     DAWKQJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.185"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1269
                     /locus_tag="WJL20_001867"
     CDS             <1..1269
                     /locus_tag="WJL20_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ7683975.1"
                     /translation="KCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            1274..1657
                     /locus_tag="WJL20_001868"
     CDS             1274..1657
                     /locus_tag="WJL20_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683976.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            1907..>2930
                     /locus_tag="WJL20_001869"
     CDS             1907..>2930
                     /locus_tag="WJL20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7683977.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALLSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAA
                     IIALAVT"
BASE COUNT          980 a          630 c          614 g          706 t
ORIGIN      
        1 aagtgtcagg atggtgcgat cggtgcggct gtgggcgaga tagtcgggga ggctttgaca
       61 aacggcaaaa atcctgccac tttgacagct aaagaacgcg aacagatttt ggcatacagc
      121 aaactggttg ccggtacggt aagcggtgtg gtcggcggcg atgtgaatac agcggcgaat
      181 gcggctaaag tcgcgattga aaataaccta ttatctcaag aagagtatgc tcttagagaa
      241 aaattgatca aaaaagccaa agggaaaggc ctattatctt tagattgggg cagcctgacc
      301 gaacaagagg caaggcagtt tatctatttg attgagaaag atcgatattc taatcaattg
      361 cttgaccgat atcaaaaaaa tccaagtagt ttaaataatc aagaaaaaaa tattcttgca
      421 tattttatta accaaacctc tggaggtaac acagcttggg cagcttcgat actgaaaacg
      481 ccccagtcaa tgggtaatct cactattcct tccaaagata ttaataacac cttatcgaaa
      541 gcctatcaaa cattgagtcg ttatgattct tttgattaca aatcagctgt tgccgcacaa
      601 cctgcacttt acttattaaa cggaccgctt ggcttcagtg tcaaagcagc tactgtggca
      661 gcaggaggat ataacattgg acagggagcg aaagcaatct ctaatggaga atatctgcat
      721 ggtacagttc aggttgttaa tggcacattg atggttgcag gatctgtatc tgcacaggct
      781 gcaatatcgg ccaagcctgc acctgttacc cgttatctga gcaatgacag tgctcctgct
      841 ttaagacaag ctttaactgc tgaaagccag agaatccgca tgaaactgcc ggaagagtat
      901 cgacaaatag ggaatcttgc gatagcaaaa attgatgtta aaggattacc gcaaaggatg
      961 gaagcattta gttctttcca aaaaggggaa catggattta tttcgttacc tgaaacaaaa
     1021 atttttaaac ctatatctgt tgataaatat cataatattg cctctcctcc tagaggaaca
     1081 ttaagaaata tagatggaga atataaatta cttgaaacta tagcacagca actcggaaat
     1141 aatcgtaatg tatcaggtag aattgatcta tttacagaat taaaggcctg tcaatcttgc
     1201 agcaatgtta ttttagagtt tagaaatcgc tatccaaata ttcaattaaa tatttttaca
     1261 ggaaaataga atcatggaaa aaataaaaaa ttatatagaa ttcaagaaat tatttaaaaa
     1321 tataacggaa aaatttgtca gagatttaga tataacatta attgaagcag ctgaatattc
     1381 tataattgaa ttcgaaattt atttatctga atatccggaa gatagtacat ttatttttac
     1441 agcatgggca atctatctgt tagaaaataa ctgtctcaat gaaagaggaa aagataattt
     1501 tttcatcgcg gaaatgaaaa aatgttatca acaaactaat ttggatagaa taaaatctat
     1561 ctattcttcg agtgactatc atatttttgc taaggatgta aacaagattc aacaaacatt
     1621 taaattaaat cttgccaata tcatgcagca agagtagcaa ctgtattttt aaccccgtcg
     1681 ggcaaaatta ccaaaaacgg taaatcacta aaaatacttt tataccgccg ccgcagcaag
     1741 ctgtagcctg catgaaacct aaaatccatg cgtaaggtgt gtgctccagc acgcacgcgt
     1801 tccatgattt acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt
     1861 atgcaagtaa atattcagat tccctatata ctgcccagat gcgtgcgtgc tgaagacacc
     1921 ccctatgctt gctgcagaac tttcgggtaa aaccggtgtg agcattagcg caccgtatgc
     1981 caatgagaac agccgcatca ttttgagtgc cgccgatata gccgcagaaa acggcaaaat
     2041 caaactgcaa tcctacggcg accaatccta ttacgccgga cagggcgaac tctatacctt
     2101 agaacgccgc agctacaaaa ccggcaaatg gtacaaccgc aaacacatta ccgaagtcaa
     2161 agaacacaaa aacgccaagc ccgacgcagt aaacctcagc gcatcccaag gtatcgacat
     2221 caaatctggt ggcagcatcg acgcctacgc caccgtattc gatgccccca aaggcagcat
     2281 taacatcgaa gccgggcgga aattgacact ctatgccgta gaagagctca actacgacaa
     2341 actagacagt cataagaaac gcaaatttct cggcattact tatgataagg tacatgatac
     2401 ctctacccaa cagatgcaaa ctgccttact aagccgtgta gtggctgaat cagctaatct
     2461 gcaatcaggt tgggatacca agttacaagg tacgcagttt gaaaccacgc tgggcggcgc
     2521 ggtcatccgt gcaggcgtag gcgaccaggc acgggccgat gccaagatta tcctcgaagg
     2581 gatcaaaagc agcatccaca cagaaaccgt aagcagcagc aaatccgccc tctggcaaaa
     2641 acaggcagga cgcggcagca acatcgaaac cctgcaactg ccaagtttca ccggtcccgt
     2701 tgcgcccgta ctgtccgcac ccggcggcta catcgtcgac attccgaaag gcaatctgaa
     2761 aaccgaaatc gaaaaactcg ccaagcagcc cgagtatgct tatttgaaac aacttcaagt
     2821 agcgaaaaac atcaactgga atcaggtgca gcttgcttac gacagatggg actacaaaca
     2881 ggagggctta accgaagcag gtgcggcgat tatcgcactg gccgttaccg
//
LOCUS       DAWKQJ010000186         2911 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000186 DAWKQJ010000000
VERSION     DAWKQJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.186"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1039)
                     /gene="siaC"
                     /locus_tag="WJL20_001870"
     CDS             complement(<1..1039)
                     /gene="siaC"
                     /locus_tag="WJL20_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ7683978.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKT"
     gene            complement(1040..1726)
                     /gene="neuA"
                     /locus_tag="WJL20_001871"
     CDS             complement(1040..1726)
                     /gene="neuA"
                     /locus_tag="WJL20_001871"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ7683979.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            complement(1730..2863)
                     /gene="neuC"
                     /locus_tag="WJL20_001872"
     CDS             complement(1730..2863)
                     /gene="neuC"
                     /locus_tag="WJL20_001872"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ7683980.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
BASE COUNT          836 a          547 c          599 g          929 t
ORIGIN      
        1 cagttttttt gatttgagca cctttgcgaa tattgcaagc agcgacctta ccaaataatg
       61 tttcatattc gttgacgctg aagtctccat tgcctgggcg tttaacccat aggttatctc
      121 cggacaacag ttctcctttt ttaatgtctt tatctgctac gacagatgca aaggcgaaat
      181 ctttagttgg cttttctccc gcgataatcg tgtctttttt gccgccgcgt gccaatttta
      241 aagcatgagc gccttgcttg agctctttaa aagtatccgg attcatagag catacaatat
      301 ccggacctgg gcgatccatg cggtcagtaa agtgacgctc taaaatcgaa ccgcctaaag
      361 ctactgctcc taagcaagca tagttatcta aggtatggtc agacaggcca atgattgcgt
      421 ctggaaaggc ttcagataaa tcgttcatac cacccaatcg aacatcttcg taaggggttg
      481 ggtagatgtt ggtacagtga agcaaagcat aaggtacccc tgcttctcga ataatttcta
      541 ccgacttttt gatgctttca atagaattca tgccggtaga gagaataata ggcttaccaa
      601 aagaggccac cagtttaatt aatgggtagt tattacattc gccagagccg attttatatg
      661 ctggaatatc catacgttgt aatcgtaaag cagctgcacg agagaaagga gtactgataa
      721 aaatcatacc cttactctct acgtattctt ttaatttaat ctcatcttct tcattcaggg
      781 cgcaacgttc cataatttca taaatagaga catctgcatt gcctggaatg acttgtttgg
      841 cctcatcaga catttcgtct tcaacgatgt gtgtttgatg tttaacaact tcagcgcctg
      901 cattataggc agcatcaacc atttcaaaag ctgtttttaa agagccttca tgattgatgc
      961 cgatttcaca gataatcaat ggttcgtggt tgtaacctac tgaacgatta ccaattttaa
     1021 attcgttgtt gttttgcatt tagctttcct tgtgattaag aatgttttct gcctgttgta
     1081 aatcaagctc agtatcaata tcgatagagt cttgatgaga cataatataa agtttggttg
     1141 gggcgataaa aaaacaatta tttgcaatta gtgaagcagt atcattaatg taaattgcac
     1201 cattaggcct aaatgcctga ggtaattgtt ggcgaggctg ctccaaatcg cttagatggc
     1261 gcatgggggc atattcgcca ttattgattt gaagcagggt ttttagtgga tgatgctcca
     1321 ttgggcatgc agagacaacg gatcctttta ttttctcatc aaatagagaa aaagcttcac
     1381 gaatatgagc ccctgtgcgt aatggactgg ttggttgtaa tagggttact gtgccggaat
     1441 tactgccaat tgtttctaaa gcatgtatta cacctgaaat agagctggct gtatcggagg
     1501 ccagctctgc agggcgtagg acgacttcga caccgaaatt tttagcttct tctgcaatta
     1561 acccgccatc agtcgaaaca attatgcggt caaaacactt tgatgatata gcagcattaa
     1621 ttgtatgacc aagtaatgat atgccattca ttttccggag attttttaat ggcaatcctt
     1681 tggagttttg gcgcgcaagt ataaccgcaa tattttgttt ttccataatt taaagattca
     1741 aatcgataaa acgtttttga gcagaaacat tccacgtttc aggattgttg attacttcag
     1801 caaatctttc tgtgctggtg cgagtatctc cgccattaaa ggtatcatct gcttcaaatt
     1861 tgcctaaact gcatgcttgt tgaatcgcat caaagatatt tttagtttca taatctgtat
     1921 gaataataga ttttcccata tggcggttac tttggcgtgt accaacatca attgaaggga
     1981 caccgtagag aggagcttct ctaatacctg cacttgagtt gccgaccata aatttagcat
     2041 gtttcaataa gactaaaaaa tattcaaatc gaatggaagg aaatgcaata aatttatcag
     2101 attgatattt taataattct tgcagaatac tttcagtgcc agtgtcatta ttagggtaga
     2161 tgctaatgat attttggcca cttaattcta atgctttgaa atattgggcc gcatattgtg
     2221 gcattaaatg tgcttctgta gtcacggggt gaaacataga aataccataa ttttcgtatg
     2281 gtaaaccgta atattctttg acttcttcta aggatgggag ggtggaagag gccataacat
     2341 ctaaatcggg ggagccgatg atgtgaatat gctttctttt ttctcccatt tgcactaggc
     2401 gagtgacagc ttgttcattt gctaccaagt ggatatgaga aagtttacta atagaatgac
     2461 gaatggagtc atctactgta ccagatagtt caccaccttc gatatggcaa actaaacggc
     2521 tgcttaatgc acctacagct gcgcctgcta gtgcttctaa acggtcgccg tgaatcatga
     2581 ccatatcagg ttcaatttca tcagatagac gagagataaa cgtaatggta ttgcctaaaa
     2641 cggcacccat tggttcacct tggatttgat ttgaaaacag atatgtatgt tgatagtttt
     2701 ctcgagttac ttccttgtag gttctgccat atgttttcat catatgcata ccagttacaa
     2761 tcaaatgcaa ttcaaggtct gggtgatttt caatataggc taataaaggt tttagcttgc
     2821 cgaagtcggc tctggtacct gtaatgcaaa gaattctttt catgatttta gaatctataa
     2881 gtatataagt ataaggaagt tggaaagaag a
//
LOCUS       DAWKQJ010000187         2892 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000187 DAWKQJ010000000
VERSION     DAWKQJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.187"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1599
                     /locus_tag="WJL20_001873"
     CDS             <1..1599
                     /locus_tag="WJL20_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7683981.1"
                     /translation="QVQLAYDKWDYKQEGLTEAGAAIIALAVTVVTAGAGAGAALGLN
                     GAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMVAVATAGVADKI
                     GASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGE
                     AASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFS
                     RMSATEIEKSKAKITAYSKLVAGTASAVVGGDVNTAANAAQIAVENNTLYPRCVGAKC
                     DEFQKEQQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADHLFALLGVVPGI
                     GESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQTELRVTAATDKQL
                     LKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKYGGNNGFDHVWQAADGSVVLIVE
                     SKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPDGSPAKAVVLKANQNGKLKTAIA
                     GVDRQTGKAVILSVKVPSKTNIRR"
     gene            1602..2537
                     /locus_tag="WJL20_001874"
     CDS             1602..2537
                     /locus_tag="WJL20_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ7683982.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          841 a          637 c          727 g          687 t
ORIGIN      
        1 caggtgcagc ttgcttacga taaatgggac tacaaacagg agggcttaac cgaagcaggt
       61 gcggcgatta ttgcgctggc tgttaccgtg gttactgcgg gcgcgggagc cggagccgca
      121 ctgggcttaa acggcgcggc cgcagcggca accgatgccg cattcgcctc gctggccagc
      181 caggcttccg tatcgctcat caacaacaaa ggcaatatcg gtaacaccct gaaagagctg
      241 ggcagaagca gcacggtgaa aaatctgatg gttgccgtcg ctaccgcagg cgtagccgac
      301 aaaatcggtg cttcggcact gaacaatgtc agcgataagc agtggatcaa caacctgacc
      361 gtcaacctgg ccaatgcggg cagtgccgca ctgattaata ccgctgtcaa cggcggcagc
      421 ctgaaagaca atctggaagc gaatatcctt gcggctttgg tgaatactgc gcatggagag
      481 gcagcaagta aaatcaaaca attggatcag cactacatag tccacaagat tgcccatgcc
      541 atagcgggct gtgcggcagc ggcggcgaat aagggcaagt gtcaggatgg cgcgataggc
      601 gctgcagtcg gtgagattgt tggtgaggct ttggttaaga atactgattt cagtcgtatg
      661 agtgcgaccg aaatcgaaaa atctaaagcg aagattactg cctattcaaa actggttgcc
      721 ggcactgcgt ctgccgttgt aggcggggat gtgaatacag cggcgaatgc ggcacagata
      781 gcggtggaga ataatacttt gtatcctaga tgcgttggtg caaagtgtga tgaatttcaa
      841 aaggaacaac aaaaatggat acgtgaaaat cctgaagaat atcgagaagt tttgcttctt
      901 cagacaggat ttattccaat tatcggtgat atacagagtt ttgtacaagc acagaccgct
      961 gccgatcacc tgtttgcttt gctgggtgtg gttccgggta tcggtgaatc gatacaggcc
     1021 tataaagtag cgaaagcggc aaaaaattta caaggcatga aaaaagcctt ggacaaggca
     1081 gcaaccgttg ccactgcaca gggctatgtc agtaaaacca aaatcaaaat cggtcaaact
     1141 gaattaaggg ttactgcagc aactgacaaa caattgctga aagctattgg cgaaggaagg
     1201 gacacgacag gtaaaatgac cgagcagtta tttgactctt tagctaaaca aaatggcttc
     1261 agagtgcttt cgggcggcaa atacggcgga aataacggtt ttgatcatgt atggcaggct
     1321 gccgatggta gtgttgtttt gattgtagaa agtaagcaga ttaggaacgg tacggtacag
     1381 ctgaatccga atggtgcggg tggatatacg cagatgagtc gtgaatggat taaacaagtt
     1441 gtaaaaagtt tacctgatgg tagtcctgct aaggcagttg tcttaaaagc aaatcagaac
     1501 ggcaaattaa aaacggcaat agcaggcgtt gatcgtcaaa caggtaaggc cgttattctt
     1561 tctgtcaaag ttccttctaa aaccaatata aggagataac aatggggcac aatatgatga
     1621 ccacccaaaa atggtatgaa catattacta atgtaatcat aggcaatact gctaatttca
     1681 atagcggttg ccccgaatct atagattatg tagatgaaaa aaaaggcgtg ccgcttgcag
     1741 cgatgaaata cattttaatg tacactgaag ctgcggcttc ccatgcctat ctatttgaac
     1801 atgatcttaa gaaattcaag caatatgctt atgttgcagg aaagttgggt attttgcaga
     1861 gtgtagatga tgaagacccc gaacccttct tctttccctg cgacatgctc aacattcaag
     1921 atccgatgtt tctgatgctg atgagcgaca gcccgcagct gcgcgagttt ttggtgcgca
     1981 atatcgacaa catcgccaac gatacagaag ccttcgtaaa ccgatacgac ctcaaccgtc
     2041 atatgattta caatactctg ctgatggtgg agggtaagca gcttgatcgg ttgaaacaac
     2101 gtagcgagaa agtcttggcg catcccaccc ctagcaaatg gctgcaaaag cggttgtacg
     2161 attaccgctt cttcctcgct ttcgccgaac aggatgccga ggcgatgaag gccgccttag
     2221 agccgctttt tgataaaaaa accgcgcgta tggctgccaa agaaacattg tcctatttcg
     2281 atttctacct gcagccgcaa atcgttacct atgccaaaat cgcatccatg cacggtttcg
     2341 atttgggcat agaccacgaa atcgcaccga gggatttgat tgtttacgat ccgctgccag
     2401 cagacgaata tcaagacatc ttcgatttta tgaaacagta tgacttgtct tacccgtatg
     2461 aatatctgca ggattggata gattactata cgttcaaaac cgataagctg gtatttggta
     2521 acgcgaagcg agagtgagcc gtaaaactct gagctcctgt tttatagatt acaactttag
     2581 gccgtcttaa agctgaaaga ttttcgaaag ctataaattg aagcccttcc acagtacata
     2641 gatctgtgtt gtggcggggc tttaccacgc tgattgccgg agaagaactc aacctgctgg
     2701 caaaacaagg catgagatct ttgcaataac atgagttgag acctttgcaa aaaagccctt
     2761 ccccgacatc cgaaacccaa acacaggatt tcggctgttt tcgtaccaaa tatccccttt
     2821 tccaccggac accccataat agagcatccg ctccgccttt taggcggcag cgggcgcact
     2881 taacctgttg gc
//
LOCUS       DAWKQJ010000188         2882 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000188 DAWKQJ010000000
VERSION     DAWKQJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.188"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(97..573)
                     /gene="greA"
                     /locus_tag="WJL20_001875"
     CDS             complement(97..573)
                     /gene="greA"
                     /locus_tag="WJL20_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ7683983.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            968..2269
                     /gene="aroA"
                     /locus_tag="WJL20_001876"
     CDS             968..2269
                     /gene="aroA"
                     /locus_tag="WJL20_001876"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ7683984.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2312..2845)
                     /locus_tag="WJL20_001877"
     CDS             complement(2312..2845)
                     /locus_tag="WJL20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7683985.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPGSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          603 a          796 c          773 g          710 t
ORIGIN      
        1 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataatt
       61 tcctgcgtgt gtcgggtgta tcgaaatcaa gccgaatcaa atatatcgga cttcgataat
      121 gtcgtattcg cgcacgccgc ccggggcttg gacttccgcc gtatcgccct cttccttgcc
      181 aattaaggcg cgggcaatcg gtgagccgac atagattttg ccctgtttga tatccgcttc
      241 gtcttcgccg acaatttggt aggtaacgtg ttcttccgtt tccaaatctt ccagcgtaac
      301 cgtcgtaccg aacacgattt tgccttcggc gtggatttcg gtcggattga tgatgtgggc
      361 aacggaaagt ttgtgttcca actcggaaat gcggccctcg ataaaacctt ggcgttcttt
      421 ggcggcttca tattcggcgt tttcggacaa atcgccgtgc gatctggctt cagcaatcgc
      481 ttcgatcact tcggggcgcg ccacgctttt aagctgctgc aattcctgtt tcagcaattc
      541 cgcaccgcgt acggtcaggg ggattttttg catcggtctt ttttctccat attccggcac
      601 accggtttgc ggcagcaaac ataccgcata ccgtcttgtt ttgtgcgtcc ggatattaaa
      661 ataaaaacac aagccgcccg gaaaatcggc ggcttgtctg tcgttgaaca gcggctattc
      721 taccaaattc tatgaaattg gcaatcgtac cgtaccgtcc gcaaacgtca tctgtccgca
      781 acaaaagccg aaaatatgcc ggcaaaaaaa ttttagaaac aaaaaattta aaaataatca
      841 attttcggca taaaaaacca catttacgga ctttaaaacc gaaaatgcca aacctgagat
      901 ttttcataca gcatttgcac cagtataatg caggctgttt ttatctttat aatattgacg
      961 ttttgccatg accgaatccc tccgcctccc tgccgcctcg ctcaaacctt ccactgtcgc
     1021 cctgcccggc tccaaaagca tcagcaaccg caccctgctg cttgccgcct tgtccgacaa
     1081 tgcttgcgaa atccattccc tgctcaaatc cgacgatacc gaccgtatgc tcgaagcact
     1141 cgataaactc ggcgttgaaa tcgaatatct tgccgaagac cgtctgaaag tgcacggcac
     1201 aggcggacgc ttccccaacc gcactgccga tttgtttttg ggcaacgcgg gcacggcgtt
     1261 ccgcccgctg accgccgcgc ttgccgtttt gggcggcgat tatcgcctgc acggcgttgc
     1321 gcgtatgcac gaacgcccca tcggcgattt ggccgatgca ctgcggattg ccggcgccga
     1381 tgtcgaatat ctcggcaagg aacactatcc gccgcttcat atcggcgaac gccaagacaa
     1441 cggcgagcgc gtgattccga ttaaaggcaa tgtgtccagc cagtttctga ccgccctttt
     1501 aatggcgttg ccgctgaccg ggcaggcgtt tgaaatccgt atggtcggcg agttaatttc
     1561 caagccctat atcgacatta ctttaaaact gatggcgcaa ttcggcgtac aggttatcaa
     1621 tgaaggctac cgcgtcttca aaattcccgc cgatgcgcac taccacgcgc ccgaacactt
     1681 gcacgtcgaa ggcgatgcct ccagcgcgtc ctacttcctc gcagccggtt tgattgccgc
     1741 cacgcccgtc cgcgttaccg gtatcggcgc aaacagcata cagggcgatg tcgcctttgc
     1801 ctgcgagctg gaaaaaatcg gtgcggacgt ggtttggggc gaaaacttcg tcgaagtctc
     1861 gcgcccgaaa gaacgtgccg tccaagcctt tgatttggat gcaaaccata tccccgatgc
     1921 cgccatgacc ctcgccgtcg tcgcgcttgc tacagggcaa acctgcacgc ttcgcaacat
     1981 cggttcgtgg cgcgtcaaag agaccgaccg cattgccgca atggcgaacg agttgcgcaa
     2041 actcggtgca aaagtcgccg aagaagccga agcaattcac atcaccccgc ccgaaacgcc
     2101 gacacccgac gccgtcatcg acacttacga cgaccaccgc atggcgatgt gtttctcgct
     2161 ggtttcgctg ttggacgtac ccgtcgtcat caacgatccg aaatgcaccc acaaaacctt
     2221 cccgacttat ttcgacgtgt tctcatcgct gaccgaaaca gcggaataag gcggcatttt
     2281 gccgcgattc cggcgcggcg cggcgggcgg ctcattctgt aaagaaagta tgtgcgccga
     2341 ggtagttttt ggcgtaaaac ggtgtggaga gtttttcggt tttgatggtt ttgccgctgc
     2401 tgggggcatg gatgaattcg ccgttgccga tatagagtcc gacgtgtgag tagcggtgtg
     2461 tgccgccggt gttgaagaat acgaggtcgc cggccttaag gcggctgccg gggattttgc
     2521 ggcttgccgc cgccatgtcg cgggcggtgc gcggcagctt gacgttgagg gcgtttttgt
     2581 aaacgaattg aatcatgccg ctgcaatcga agccggttgc ggtgctgctg ccgccccatt
     2641 tgtagggcgt gccgatgagt ccgaggctgt ggagcatgag ttcctgcgag ccttgtgtgc
     2701 ggtcgatgtg gctgatgcgg acggcttgga tttgccggac tgtctgtttg ggtttcggtt
     2761 ggcggtgttt gccggaggtt gtgccgcatg aggcgaggag cagtgcgctg agacagagga
     2821 aaagggtttt gtcgggggga aacatggttt ttcctttgcg ggttcggata tccgtctgaa
     2881 gg
//
LOCUS       DAWKQJ010000189         2820 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000189 DAWKQJ010000000
VERSION     DAWKQJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.189"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..1247
                     /locus_tag="WJL20_001878"
     CDS             30..1247
                     /locus_tag="WJL20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7683986.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1450..1590)
                     /locus_tag="WJL20_001879"
     CDS             complement(1450..1590)
                     /locus_tag="WJL20_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683987.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1603..2731
                     /locus_tag="WJL20_001880"
                     /pseudo
     CDS             1603..2731
                     /locus_tag="WJL20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          664 a          845 c          671 g          640 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac catcaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggcatatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgcagtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcacaggc tcaggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgat tctgcccgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcctcga catccatcgc catcatcgga ctggcaaaca
      841 tcgccggctg cattttttcc ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgacgatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttc gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcccgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg ataatgaatt ggatattatc
     1741 agggaaatgg gggttacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatcttt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgccgcaaa actggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcagccggaa
     1981 ggcataccgt taaaaacaaa aatcataaag gggattttga ttgccttgta ccacattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggatttgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa atttctcttt
     2221 atcggcagat ttctgaaaga aaaagggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aagggcaaat accccgatac ggtttttacc gctttgggcg caatcgacaa atcacgcggg
     2341 gggggggggc gatttggaac ggcttgccgc ccgcgatatt atccgtttcc ccggttttgt
     2401 gaacaatgtt tccgaagtga taaaggcgca tcatatattc gtattgccgt cttataggga
     2461 aggcgttccc cgaagcactc aggaggcaat ggccgtcggc agggcggtga ttacgacgga
     2521 tgtccccgga tgcagggaaa cggtcgccga caaggtcaac ggcttcctga tcgaaccttg
     2581 gaatccccgc atcttggccg aaaaaatgat ttattttatc gaaaacaggg aagccgtccg
     2641 cctgatgggg aatgcaagtt atgcgattgc caaagataaa ttcgatgccg aaaaagtcga
     2701 tttgaaattg ctcgatattt tgaaggcgta aacaaggctg cccgcttttg agtttcggtc
     2761 atttctgata aacccccgtc attcccgcca cacccccgtc attccctcga aagcgggaat
//
LOCUS       DAWKQJ010000190         2757 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000190 DAWKQJ010000000
VERSION     DAWKQJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.190"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..437
                     /locus_tag="WJL20_001881"
     CDS             114..437
                     /locus_tag="WJL20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7683988.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            551..2728
                     /locus_tag="WJL20_001882"
     CDS             551..2728
                     /locus_tag="WJL20_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7683989.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          667 a          984 c          690 g          416 t
ORIGIN      
        1 gtaagccgca ggcttacatc aaagccgata acgcttccgt tacaactccg cccactgaac
       61 gaagccaaaa cgacaaaccg catcgtaaac cacccaaccc ataggagaac cccatgcaaa
      121 acgaaaccat caacctgaaa cagcaccttg ccgccatcaa agaatactgg cagcccgaaa
      181 tcatcaaccg ccacgggttc caattccgtt tggtcaaact tttgggcgat tacggctggc
      241 atacgcacgg atacagcgac aaagtgctgt ttgccgtgga gggcgacatg gcggtggact
      301 tcgccgacgg cggcagcatg acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg
      361 tgtcgcaccg cccgcgttcg gaaaacggct gctcgttggt gctgattgag ttgtccgacc
      421 cgtccgaggc cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa
      481 gcagcctgca ccttcaatca atatgccgaa aatacaaccc caccgcacac caacacacaa
      541 aggaaatccc atgacacgct tcaaatattc cctgctgttt gccgccctgt tgcccgtgta
      601 cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca ctgaattgcc
      661 gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca ctgtttccgg
      721 cacgcacacc ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt
      781 catcacatcg caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca
      841 ggcgaccggc accagccgcc agatttacgg ctccgaccgc gcgggctaca actacctgtt
      901 cgcgcgcggc agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct
      961 ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt
     1021 ggcggggctg ctggacggca cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg
     1081 cccgacccgc aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg
     1141 gctggacgcg gacgtatcgg gcagcctgaa caccgaaggc acgctgcgcg gccgcctggt
     1201 ttccaccttc ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact
     1261 ctacggcatt ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta
     1321 ccagcaggcg aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg acagccaagg
     1381 ttatgccacc gccttcggcc cgaaagacaa ccccgccaca aattgggcga acagccgcca
     1441 ccgtgcgctc aacctgtttg ccggcatcga acaccgcttc aaccaagact ggaaactcaa
     1501 agccgaatac gactacaccc gcagccgctt ccgccagccc tacggcgtag caggcgtgct
     1561 ttccatcgac cacaacaccg ccgccaccga cctgattccc ggttattggc acgccgaccc
     1621 gcgcacccac agcgccagcg tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca
     1681 cgatttaatc gcgggtatca acggttacaa atacgccagc aacaaatacg gcgaacgcag
     1741 catcatcccc aacgccattc ccaacgccta cgaattttcc cgcacgggtg cctacccgca
     1801 gcctgcatcg tttgcccaaa ccatcccgca atacggcacc aggcggcaaa tcggcggcta
     1861 tctcgccacc cgtttccgcg ccgccgacaa cctttcgctg atactcggcg gcagatacag
     1921 ccgttaccgc accggcagct acgacagccg cacacaaggc atgacctatg tgtccgccaa
     1981 ccgtttcacc ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt ctctttacgg
     2041 ctcgtacagc agcctgttcg tcccgcaatc gcaaaaagac gaacacggca gctacctgaa
     2101 acccgtaacc ggcaacaatc tggaagccgg catcaaaggc gaatggcttg aaggccgtct
     2161 gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg
     2221 cgacctgagc ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga
     2281 aatcgaagtc ggcggccgca tcactcccga atggcagata caggcaggtt acagccaaag
     2341 caaaacccgc gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt
     2401 caaactcttc actgcctacc actttgcccc cgaagccccc agcggctgga ccatcggcgc
     2461 aggcgtgcgc tggcagagcg aaacccacac cgaccctgcc acgctccgca tccccaaccc
     2521 cgccgccaaa gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat
     2581 catggcgcgt taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg acaacctgtt
     2641 caacaaacac taccgcaccc agcctgaccg ccacagttac ggcgcactgc ggacagtgaa
     2701 cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaag
//
LOCUS       DAWKQJ010000191         2671 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000191 DAWKQJ010000000
VERSION     DAWKQJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.191"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            237..1196
                     /locus_tag="WJL20_001883"
     CDS             237..1196
                     /locus_tag="WJL20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683990.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1244..2107
                     /gene="galU"
                     /locus_tag="WJL20_001884"
     CDS             1244..2107
                     /gene="galU"
                     /locus_tag="WJL20_001884"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7683991.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2190..>2671
                     /locus_tag="WJL20_001885"
     CDS             2190..>2671
                     /locus_tag="WJL20_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7683992.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     T"
BASE COUNT          832 a          471 c          536 g          832 t
ORIGIN      
        1 acttaatatc taatatttaa aatacaatga ataaaaaacg agtcctatta attgctagtt
       61 atgattcttt tttgaatact ggatatgctg ttgcaaaaga aattaactat tcaacaatcg
      121 atatttatat ccaccaaaca aaaaaaaata ttctttcgga atcacaatta agtttaaatg
      181 ggctttcttt aaaagaaatc aatttttttc atattgatga ttatttcgac aaattaatgt
      241 accagaatta tgatattgtt attctatcat taggtaatgg tttactgacg cgtttttttc
      301 ggaatagtag caaacttaat ttaacattac ctatagtaat tactctattt ccaggggtaa
      361 tttttggtga ttcagaaagt attataacta ggttaggtgc taatatttta ttgttcaata
      421 attattatga ttaccaaata ggcaataagt tcaaagaacg ttacaactta tcgtgtacaa
      481 atattttata tggttatcct atcctacgaa aaaaacacca ttgctctgat ggcaataaag
      541 tttatttcat tgatcaagta aaaattccag atggaaaaaa agaaagatta ttaattctaa
      601 aaaaattaat agcactggct aatcgctatc cacataaaga tttagtacta ttattacgtg
      661 tccatcaaaa tgatatcaca gtacatgaag ataaatactc ttatgctgaa ttagcaaaaa
      721 atatgaaatt gcctgataat tttaaaatag agagaaaacg gacacaagat gccttacaag
      781 aaatgggata ttgtttatca ttctcttcaa caatgttatt tgaagctgaa agctgcggaa
      841 ttcctattgg tgtcattagt gatttagggg ttgacaagaa atatgccaat acacatttca
      901 aagatagtgg tgttttggtt aatttagata cggtagactt ggactctcca aataccatta
      961 atccaacttg ggctgaacta tatactaatc cagatagcct ctcttcaaaa gagtttaacc
     1021 aattattaga ggttacttct actcaaccac ctagttttga taattatttt attgcagtta
     1081 atgacgttca gcctgtatct atggttctgt tacgtaaatt taaaaagctt atatatcatc
     1141 ctaagcaatt ctttgttgat tcaaaactat ataaattaat taaatttact aaataatttt
     1201 tactatgggt tgccttattt atttcaaagg agtgacataa aaaatgcaaa aaattcgtaa
     1261 agctgtattc cctgtagctg gcatgggaac ccgattctta cctgccacga aagccagcgc
     1321 taaagaaatg ctgccaatcg tggacaaacc gttaattcaa tatgctgcgg aagaagccgt
     1381 tgccgcgggt tgtactgaat tgatttttat caccggccgt aataagcgta atattgaaga
     1441 ccacttcgat aaagcctatg agttggaaac tgaactggaa atgcgtgaaa agcataaatt
     1501 gcttggctat gtgcaaaaaa ttctgcctga tcatgtcagc tgtattttca ttcgtcaggc
     1561 agaagctttg ggcttgggtc atgcggtatt atgcgcccgt cctgctgtcg gtaatgagcc
     1621 ttttgccgtg attttggccg atgacttgat tgatgcacca caaggcgcac tcaagcagat
     1681 ggtagatatt tacaatcaaa gcggcaacag cattttaggg gtagaaacag tcgatccttc
     1741 gcaaaccggt tcatacggca ttgtcgaagt ggaacagctg aaaagctatc agcgtgtaac
     1801 caatattgtg gaaaaaccaa agcctgaaga tgcgccgtca aatttagcgg tagtcggccg
     1861 ttatattttg acaccgcgta tttttgattt gctgactaat ctacctcgtg gtgcaggtgg
     1921 tgaaatccag ctaactgatg gtattgcccg cctacttgac catgaatttg tcttggcgca
     1981 tgcgtttgaa ggtaagcgtt atgattgtgg cagtaagttg ggctatctgg aagccacggt
     2041 agcttatggc ttgaagcatc ccgaattaaa agatgatttc aaagaattat tgaagcaatt
     2101 tgtataattt taaggccgtc tgaaaaagaa aaggcatcat gttttttaca ctttcaggcg
     2161 gccttttaaa cataaagaag gaaaacgtga tgaacattct cgttaccggc ggcaccggtt
     2221 ttatcggctc gcacaccgtt gtttctttgc tgaaaagcgg tcatcaagtc gtgattttgg
     2281 ataacctatg caattccagc atcaatatcc tgccacgctt gaaaacgatt accggccaag
     2341 agattccgtt ttatcaaggc gatatccgcg atcgtgagat tttgcgccgt atttttgcgg
     2401 aaaaccgcat tgattcggtg attcattttg ccggcttgaa agcggtgggt gaaagtgtgg
     2461 ccgagccgat aaaatattat gataataatg tttccggcag cttggtgttg gcggaagaaa
     2521 tggcgcgtgc gggcgtgttt agcattgtgt tcagttcttc ggcgacggtt tatggcgatc
     2581 cgggcaaagt gccttatacc gaggatatgc caccgggcga caccaccagc ccttacggcg
     2641 catcgaaatc gatggttgag cgcattctca c
//
LOCUS       DAWKQJ010000192         2664 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000192 DAWKQJ010000000
VERSION     DAWKQJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.192"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            78..641
                     /gene="gmhB"
                     /locus_tag="WJL20_001886"
     CDS             78..641
                     /gene="gmhB"
                     /locus_tag="WJL20_001886"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ7683993.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            670..1413
                     /locus_tag="WJL20_001887"
     CDS             670..1413
                     /locus_tag="WJL20_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7683994.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1410..2102
                     /locus_tag="WJL20_001888"
     CDS             1410..2102
                     /locus_tag="WJL20_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7683995.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLAPTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYADEAKQW
                     LKDRGRELFALG"
     gene            2141..2326
                     /locus_tag="WJL20_001889"
     CDS             2141..2326
                     /locus_tag="WJL20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7683996.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT          644 a          883 c          645 g          492 t
ORIGIN      
        1 cacgcggcac aggcgcatca ctgccgatat ttcagtataa tgccatcccc gacctgcccc
       61 aacccaaagg aaccgcgatg aaactcatca ttctcgaccg cgacggcgtc atcaatcagg
      121 accgcgatga cttcgtcaaa tccgttgacg agtggatacc tgtcgaaggc agcatggatg
      181 cggtggcatt tctgacgcag gcaggctaca ccgtcgccgt tgccaccaac caatccggca
      241 tcgggcgcaa atattttacc gttcaaaacc ttaccgaaat gcacgccaaa atgcaccgcc
      301 tcgtccgtca ggcaggcggc gaaatcaacg gcatctggtt ctgcccgcac accgatgccg
      361 acaactgcaa ctgccgcaag cccaaaccgg gtatgattga agacatcatc ggacgcttca
      421 acgcccaagc ctcggaaacc tggctggtcg gcgacagcct gcgcgatttg caggcaatcg
      481 atgccgtcgg cggaaaaccc gcgctggttc tgaccggaaa aggcaaaaaa acgctctccc
      541 aacacggaca cgaattaccc gaacacacac aggttttcga taccttgctc gatttctcac
      601 aatacatcat gcaggaaaac gccgcaccgc aagccgactg aacataccgc attccgacaa
      661 ggcaaaacca tgctcatcat ccgcaacctg atttactggc tgatactctg ttccaccctg
      721 attttcctct ttccctttat gctgctcgcc tcgcctttcc gagacggggc gcacaagatg
      781 gcgcgggtct gggtcaaaat cctcaacctc tcgctcaaac acatcgtcgg gctcaaatac
      841 cgcatcatcg gcgcggaaaa catccccgac cgccccgccg tcatctgcgc caaacaccaa
      901 agcggctggg aaacgctcgc ccttcaggac atttttccgc cgcaggttta cgttgccaaa
      961 cgcgagttgt tcaaaatccc ctttttcggc tggggcttga aactggtcaa aaccataggc
     1021 atagaccgca acaaccgccg cgaagccaac gagcagctca taaaacaggg gttggcgcgc
     1081 aaaaacgaag gctattggat taccattttc cccgaaggca cacgccttgc gcccggaaaa
     1141 cgcggtaaat acaaactcgg cggcgcgcgc atggcgaaaa tgtttgagat ggacatcgtc
     1201 cccgtcgccc tcaacagcgg cgaattttgg ccgaaaaact cctttctgaa atatccgggg
     1261 gaaatcaccg tcgtcatctg tccgaccatc ccgcacgcaa gcggcagcga agccgaattg
     1321 atgggaaaat gcgaacacct catcgaaacg cagcagccgc tcatttccgg cgcaggcccg
     1381 tttgccgcca aaatgccgtc tgaaaccgca tgaccgcctt tgtccacacc ctttcagacg
     1441 gcatggaact gaccgtcgaa atcaagcgcc gtgccaagaa aaacctgatt atccgccccg
     1501 ccggcacaca taccgtccgc atcagcgtcc caccctgctt ctccgtctcc gctctaaacc
     1561 gctggctgta tgaaaacgaa gccgtcctgc ggcaaacact ggcgaaaaca ccgccgccgc
     1621 aaactgccga aaaccggctg cccgaatcca tcctcttcca cggcagacag cttgccccta
     1681 ccgcccatca agacacgcaa atcctgctga tgccgtctga aatccgtgtt cccgaaggcg
     1741 cacccgaaaa acagcttgcg ctgctgcggg actttttgga acggcaggcg cacagttacc
     1801 tgattccccg cctcgaacgc cacgcccgca ccacacaact gttccccgcc tcctcctcgc
     1861 tgacctctgc caaaaccttc tggggcgtgt gccgcaaaac cacaggcata cgcctcaact
     1921 ggcggctggt cggcgcaccg gaatacgttg ccgattatgt ctgcatacac gaactctgcc
     1981 acctcgccca tcccgaccac agccccgcct tttgggaact gacccgccgc ttcgccccct
     2041 acgcggacga agccaaacaa tggctcaaag accgcggcag ggaacttttc gccttaggct
     2101 gacgcggacc ggaccgaccc gccgctttca gacggcatcc gtgccggaac aggcacgcgc
     2161 ccgcccgatt caaaccgcga tgacgctttg ccgccggttc ggggcaggat ggcggcacac
     2221 gcgccgtctg ccgcgtttca tttcacaccg ctcttccgaa acccgaaacc cgcccggtcc
     2281 gacgtgcggt atgaaacgct taagctggcg cgaagtcttt tactgatttg cccgcgaaaa
     2341 tgccgtctga aaggttttcg gacggcattt ttttgcgttt cccaggatgg cggcggattc
     2401 gtaaaaggcg gtcagggtgg attgtaggat gggttgagac ctgccgaatc cgccgcatct
     2461 gccaaatcta ccgccgtcat tcctacgaaa gtgggaatct agaacgcggg gttaagaaaa
     2521 cctacatccc gtcattccca cgaaagtggg gatctagaac gcggggttaa gaaaacctgc
     2581 atcccgtcat tcccacgaaa gtgggaatcc aggacgaaaa atctccagaa accgttttac
     2641 ccgataagtt tccgcaccga caga
//
LOCUS       DAWKQJ010000193         2613 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000193 DAWKQJ010000000
VERSION     DAWKQJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.193"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            74..703
                     /locus_tag="WJL20_001890"
     CDS             74..703
                     /locus_tag="WJL20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ7683997.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            849..2522
                     /locus_tag="WJL20_001891"
     CDS             849..2522
                     /locus_tag="WJL20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ7683998.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGNADFGIPPVYLWVAAAFKHLLSPWVADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDTDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGLHTRIVWTQYGTLPHRVGDVQ
                     CRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKTGENILKTTD"
BASE COUNT          516 a          660 c          686 g          751 t
ORIGIN      
        1 tggggttgaa gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg
       61 aatgagatgt tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc
      121 cgtagactta cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat
      181 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      241 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      301 tccaattttc ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc
      361 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      421 tgcaagttgt ggtgctgaca atattggtgt ctttgttcca tattttacta ccttaaattt
      481 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
      541 tgcccaaaaa ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg
      601 gtttgttgct gttgtttatt tgggattggg ggtatatatc ctgattgaaa acaacagttt
      661 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
      721 aagactgctc cgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
      781 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
      841 tcacgcttat gctgacctat accccgcccg atgcccgccc gcccgccaaa acccacgaaa
      901 aaccgtggct gctgctgttg atggcgtttg cctggttgtg gcccggcgtg ttttcccacg
      961 atttgtggaa tcctgacgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
     1021 ccaccccctt ggttgcccat ctgttcggga atgccgattt cggcataccg cccgtgtatc
     1081 tttgggttgc cgccgcgttc aaacatttgc tgtcgccgtg ggttgccgac ccgtatgatg
     1141 ccgcacgctt tgccggcgtg tttttcgccg ttgtcgggct gacttcctgc ggctttgccg
     1201 gtttcaactt tttgggcaga caccacgggc gcagcgttgt tttaatcctt atcggctgta
     1261 tcgggctgat tccggttgcc catttcctca accccgccgc tgccgccttt gccgccgccg
     1321 gactggtgct gcacggctac tcgctggcac gccggcgcgt gattgccgcc tctttcctgc
     1381 tcggtacggg ttggacgctg atgtcgctgg cggcagctta tccggcggca tttgcgctga
     1441 tgctgcccct gcccgtgctg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     1501 cggcagtcgc ctcgcttgcc tttgccctgc cgctgatgac cgtttatccc ctgctgttgg
     1561 caaaaacgca gcccgcgctg ttcgcgcaat ggctcgacta tcacgttttc ggtacgttcg
     1621 gcggcgtgcg gcacgttcag acggcattca gcttatttta ctatctgaaa aacctgcttt
     1681 ggtttgcatt gcctgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt
     1741 cgaccgactg ggggattttg ggcgttgtct ggatgcttgc cgttttggtg ctgctcgccg
     1801 ccaatccgca gcgttttcag gacaatcttg tttggctgct gccgccgctt gccctgttcg
     1861 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca
     1921 ttatggcgtt cggactgttt gccgtgttcc tgtggacggg cttttttgcg atgaattacg
     1981 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcctgaca
     2041 ccgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2101 cccggaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2161 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2221 ccgtcgtccg gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg
     2281 gcatcgagtg tatcggcata ggcggcggcc tgcacacgcg gattgtttgg acgcagtacg
     2341 gtacattgcc gcaccgcgtc ggcgatgtac aatgccgcta ccgcatcgtc cgcctgcccc
     2401 aaaacgcaga tgcgccgcaa ggctggcaga cggtctggca gggtgcgcgc ccgcgcaaca
     2461 aagacagcaa attcgcactg atacggaaaa ctggggaaaa tatattaaaa acaacagatt
     2521 gagccgaatt tctggattaa gtgccggaaa ttgcgtataa ttgcgcaatt aaacctttat
     2581 atagtggatt aacaaaaatc aggacaaggc gac
//
LOCUS       DAWKQJ010000194         2579 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000194 DAWKQJ010000000
VERSION     DAWKQJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.194"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(195..266)
                     /locus_tag="WJL20_001892"
     tRNA            complement(195..266)
                     /locus_tag="WJL20_001892"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Arg,seq:cct)
     gene            complement(286..732)
                     /locus_tag="WJL20_001893"
     CDS             complement(286..732)
                     /locus_tag="WJL20_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ7683999.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(737..1711)
                     /gene="ispB"
                     /locus_tag="WJL20_001894"
     CDS             complement(737..1711)
                     /gene="ispB"
                     /locus_tag="WJL20_001894"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ7684000.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQIANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1727..1915)
                     /locus_tag="WJL20_001895"
     CDS             complement(1727..1915)
                     /locus_tag="WJL20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684001.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1935..2243
                     /gene="rplU"
                     /locus_tag="WJL20_001896"
     CDS             1935..2243
                     /gene="rplU"
                     /locus_tag="WJL20_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ7684002.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2268..2540
                     /gene="rpmA"
                     /locus_tag="WJL20_001897"
     CDS             2268..2540
                     /gene="rpmA"
                     /locus_tag="WJL20_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ7684003.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          755 a          692 c          576 g          556 t
ORIGIN      
        1 taaaacaaca gatttccaag ccggaacatc ttttacggag cctgaaaatc atttcatgat
       61 tctaccgtcc taaaggtcgg tttttcaagc aggaagaaaa agttttcaga tggcaaaaaa
      121 gccctccaac actgaaaggc cttatatcgg aaacttcccg caacacggga aacagacaaa
      181 tgcaaatcgt caaacctcgc caacaggaat cgaacctgta ttttacgctt aggaggcata
      241 cgttctatcc gttgaactat ggcgagccga aatgaaaagg agattttaac cctttccgac
      301 gacaaaagac aagatgccgg ccgcaatcag cggcccgaca gggataccgc ccataaatgc
      361 caccccgata accgtcccga ttaacagccc tgtaattaaa acaggctgct gtcccatcaa
      421 aggcacgccg cgtcccgcca gccaagccac gaaaataccg ataaaaacgg cggatatcat
      481 tttaaaattc aaaaattcgg caacaggagg aacctgcgcc tttcctgaaa ccaacggact
      541 caaaacccct atggtcaaaa gaatgatacc gagattcaac ccgtgcttct cgaccaacgg
      601 gacaaactgt accaatgccg tctgctgcat cagcagcaat atggttgccg agatggtaat
      661 cgaattgttg ttgctgacca cccccaacag aatcagcgta accagaaaca ggggaacaaa
      721 actgaaattc atcgcctcaa gacaccctga ccaaagattc cttcgccagc tgaatcatgg
      781 catccttcac ttcgctgtcg ggcagggcat ccaaggcggt aacggcacaa tcgactgctt
      841 tgcgcgcctc gcctatcgaa tatgccaacg catccgaacg gacgacataa tcgtggattt
      901 tctcaaaata gctgcgatct gcattttcca aagcagtacg cacatcgttc gcaatctgtt
      961 cggaaccctg acgcatcaga taaatcaaag gcaaagtcgg ttttccttcc gccaaatcgt
     1021 cgccgacgtt tttgccggtt tcttcggttt cgccagaata gtccagcaca tcgtcaataa
     1081 tttggaatgc cgtgccgacg tacataccgt agtctttcaa cgcctgttcg tgttcggggg
     1141 aagccttgcc caaaattgcg ccgacttgag cggcagcttc aaacaatttt gccgttttat
     1201 attggataac ttggatatat tgttcttcgg taatgtccgt attgccgatg ttcatcagct
     1261 gcatgacttc gccctcggca ataatgttgg ttgcatccgc catcacttcc aaaacgcgca
     1321 tactgcccga ggcaaccatc agttgaaagg cgcgcgtgta taaaaagtcg ccaaccaaca
     1381 cagccgccgc attgccgaac agattgtttg ccgttgcccg cccacggcgc aaatcgcttt
     1441 catcgacgac atcgtcgtgc aggagggtgg aagtgtggat aaactcgacc atcgccgcca
     1501 gcgaatacag tttctcgtca tcataaccga ccgccttacc cgccaaaatc gtcataatcg
     1561 gacgcaggcg tttgccgccc gcgctgatga tatatgtacc gatttgcgaa atcagtgcga
     1621 catcggattg caccgcacgg ttgatgactt cattgacttt ggcaaggtct tcaggcagat
     1681 gtcgctggaa atagggcaga ttctcgagca tggcagactc tcaggtttac gtttccgtca
     1741 aacggcagat accgcttgaa agctgattca aatagcgggc aattatatca gcaaagccat
     1801 cagggtacat cttttcagac ggcatatggt tatccgaacc gcgcaaactt tgacaaaaca
     1861 ggcaaataac aatacaatga cgagttccgc acaatggtgt gcggatatta accataaccc
     1921 ttatggagtt gagtatgtac gcggtcgtaa aaaccggcgg caaacagtat aaagtttccg
     1981 tcggcgaaaa attgaaagta gaacagatac cagccgaact cgacagccaa atcgaactga
     2041 ccgaagtttt gatgattgct gacggcgaat ctgtaaaagt tggcgcaccc tttatcgaag
     2101 gtgcaaaagt aacggctaaa gtagtggcac acggtcgtgg cgaaaaagtc cgcatcttca
     2161 aaatgcgccg ccgcaaacac taccaaaaac gccaaggcca ccgccaaaat ttcacccaaa
     2221 tcgaaatcgt ggcaatcgcc taaccttaaa acttaggagc attacaaatg gcaagtaaaa
     2281 aagcaggcgg cagcacccgc aacggtcgcg attcagaagc caaacgcttg ggcgttaaag
     2341 cctacggcaa cgagctgatt cccgcaggtt ccatcatcgt acgccaacgc ggtaccaaat
     2401 tccacgcagg cgacaacgta ggtatgggca aagaccacac tttgttcgcc aaagttgacg
     2461 gttatgtcga attcaaaacc aaaggcgcgc tgaaccgtaa aactgtcagc atccgtcctt
     2521 acaccggttc tgaagaataa tccaaccgat acctgaagcc gcatcttttc acgatgcgg
//
LOCUS       DAWKQJ010000195         2506 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000195 DAWKQJ010000000
VERSION     DAWKQJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.195"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..819)
                     /locus_tag="WJL20_001898"
     CDS             complement(13..819)
                     /locus_tag="WJL20_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ7684004.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="WJL20_001899"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="WJL20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ7684005.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          699 a          627 c          649 g          531 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taagtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattctga tttatagtgg attaacaaaa atcaggacaa ggcggc
//
LOCUS       DAWKQJ010000196         2490 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000196 DAWKQJ010000000
VERSION     DAWKQJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.196"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(37..993)
                     /locus_tag="WJL20_001900"
     CDS             complement(37..993)
                     /locus_tag="WJL20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7684006.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAARPLLQRPFESDGFGLLADEAATLELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     TLIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1229..2194
                     /locus_tag="WJL20_001901"
     CDS             1229..2194
                     /locus_tag="WJL20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7684007.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          529 a          668 c          784 g          509 t
ORIGIN      
        1 ttgtttcaaa tcaaaatatc tatgccgccg gccccatcag cacttcttca catccgaagg
       61 caaaaatccg taatgccgtc tgaacgcttc gttgaaccgt cccgcgtggc ggtagccgca
      121 aaagtgcatg gcggcttgga cggtgctgcc ggattcgatg agggtgagcg cgtgttccag
      181 ccgcaggcgg cgcaggcatc cggcgacggt ttcgccggtt tgcgctttga aatagcgttt
      241 caggtagcat tcgttcagtc cgacgcggcg ggcgatttcg gcgatggtca gcggacgggc
      301 gaattcgtgt tgcaggatgt cggcggcttc gtctatgcgc cgacggcggt aaccgttgtc
      361 gtggcggcgg aaggtgaagc gcaataatcg ggcggagagt tccagcgtgg cggcttcgtc
      421 agcaagcagg ccgaagccgt ccgattcgaa cgggcgttgc agcagtgggc gcgccgccgc
      481 cgtcagtgcc gccgcgtttt gcgccagccg ttgcagggcg aatcggccta tcgtttgcgg
      541 cgaaaacagg cgttcgtcca gcaagccttc gtcgtgccag cggcgcagtt tttccagcga
      601 aaaatccaaa tgcagcgcgc acatgccgct gttgtcgggc agcagggttt cggatacgtc
      661 cgccaactcg ccgcgtacca gccagatttc gccggcagac gggcggtatt ccctgccgcc
      721 catttgtaac cggttctgcc ccgacaccat gacgaacaag gcgcagttgt ggctgaaatt
      781 gtggatttcg gtgggaaacg cgcccgttcc gccgccgcgc atccgcgaca aggtgatgcc
      841 cgagtcgaag cggttgatgc acatttccag atgcaaaccg ggctgttttg cctgcgcaat
      901 gagcgcgctg tcggaacagc cgtccaacgc ccagccggat ttatcggagc ggacataggt
      961 ttggtactgg cggtagatgg cggcggtgtt catgattgga taggaacgag ttgtctaaca
     1021 aatgaattaa ataggaatta ttaccaataa tcaagcgcag ggattggttg aaacggaaaa
     1081 ggtcgtctga aagggtgttt cagacgacct tttccgtatc gggaatttgt tttgccgtat
     1141 cgggaatttt gcgttttgcg gcgtggtttc tgcaggttgt ttgcttaata ataaacattc
     1201 ttattcgtat gcaaaggaac cgcacaccgt gaaaccgcgt ttttattggg cagcctgcgc
     1261 cgtcctgctg accgcctgtt cgcccgaacc tgccgccgaa aaaactgtat ccgccgcatc
     1321 cgcatctgcc gccacactga ccgtgccgac cgcgcggggc gatgccgttg tgccgaagaa
     1381 tcccgaacgc gtcgccgtgt acgactgggc ggcgttggat acgctgaccg aattgggcgt
     1441 gaatgtgggc gcaaccaccg cgccggtgcg cgtggattat ttgcagcctg catttgacaa
     1501 ggcggcaacg gtggggacgc tgttcgagcc cgattacgaa gccctgcacc gctacaatcc
     1561 tcagcttgtc attaccggcg ggccgggcgc ggaagcgtat gaacagttgg cgaaaaacgc
     1621 gaccaccata gatctgacgg tggacaacgg caatatccgc accagcggcg aaaagcagat
     1681 ggagaccttg gcgcggattt tcggcaagga agcgcgcgcg gcggaattga aggcgcagat
     1741 tgacgcgctg ttcgcccaaa cgcgcgaagc cgccaaaggc aaaggacgcg ggctggtgct
     1801 gtcggttacg ggcaacaagg tgtccgcctt cggcacgcag tcgcggttgg caagttggat
     1861 acacggcgac atcggcctac cgcctgtaga cgaatcttta cgcaacgagg ggcacgggca
     1921 gcctgtttcc ttcgaataca tcaaagagaa aaaccccgat tggattttca tcatcgaccg
     1981 taccgccgcc atcgggcagg aagggccggc ggctgtcgaa gtattggata acgcgctggt
     2041 acgcggcacg aacgcttgga agcgcaagca aatcatcgtc atgcctgccg cgaactacat
     2101 tgtcgcgggc ggcgcgcggc agttgattca ggcggcggag cagttgaagg cggcgtttga
     2161 aaaggcagaa cccgttgcgg cggggaaaga gtagggagtc gtctgaaaac ggagcttccg
     2221 aaggaagcgg ggggggtttc tgcgaagcta aagtacggtt tcaacgaatt gaaaagcagc
     2281 ctgtatgttg aaaataccgc tcaagcaaac ctgcggtttg ccgccctctc cctagccctc
     2341 tcccacaggg agaggggatt ggggtgcagg ctgtctttaa ggtttaggca aatttttaac
     2401 ttcgttgaag ctccgttttc agacgacctt ttggagtacc gcaggcacac gcatcgaacg
     2461 gctgaatcaa agttcagacc gatggcagcc
//
LOCUS       DAWKQJ010000197         2455 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000197 DAWKQJ010000000
VERSION     DAWKQJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.197"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1571)
                     /locus_tag="WJL20_001902"
     CDS             complement(120..1571)
                     /locus_tag="WJL20_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ7684008.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1594..2214)
                     /locus_tag="WJL20_001903"
     CDS             complement(1594..2214)
                     /locus_tag="WJL20_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ7684009.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          770 a          580 c          572 g          533 t
ORIGIN      
        1 aggaataaga attcgggaca aaatgtttaa accattttgt cccgactgct gtgatgcggt
       61 ttttttgaac tagcaggacg ttacctaaaa cctgcgttgc ctgaaaccct ttaagacggt
      121 taaatccctc tgccgtattt gtattctaca atcatattat cgtcggtaat gacttccgcc
      181 ccagccgaag gttccgtcat ctgaatcagc atacgagaga caaccttttg tgctgcagca
      241 tccacggtgc tgctgtcaaa tacgtgcctg ccgctttccg gccaaatcaa ccgggagaga
      301 cgttgcttga gcagttcttt attagggaaa actaccgggg ttgccgagcc gactaccata
      361 tgcccatagc ggtatgcata gggaatactg tgtacggcgg tagcaaaagc atgcgggctg
      421 tgcgtggtat taaacattac aataccatcc ggggtaaggt ggctttgcac ctgttttaaa
      481 aattccgcac tcaacaggtt ggtggaatag gcacgccagt accaagtcgt attcatcaaa
      541 atcaggtcga atttttcatc aggatggcga cgcagccatt tcctaccgtc atccaataca
      601 atttcaacac gtttgtcctg caaaagcggg gcgatttgcg gctcgtccgc gataaggcta
      661 cggtatgccg gattgatttc cgcaacaatc atcgactgca tttccggaat ggcagacaag
      721 acgcgcgccc acgaacctgt actcaatcca acgacgaaaa tgcggcgtat gccagacttc
      781 agggagggta gcagataggc acgttcgatg ccgttgacac tattgaatac atcggtattg
      841 tatgcgccgt cgtatacatt cgccccataa acaaccttat caccatctct atggtaaacc
      901 gcaacaatgc cgtgtttgtt ttcaatcagc ctatccggac ggtcagcaat attttgaaag
      961 acagaatccg gcagtaggaa catgaggatg ccgaacatta gggaaactgc taccgacact
     1021 gcattcagtc ggagactttt ttggaacagt gtacaaaaca aagggacagc agcagaaatc
     1081 aaacagatga gcaggtaaat ctgttgggtg gacaagaaat caagtatcac aaagccgata
     1141 aggaccggac ccaatgcact gccggcaacg ttggcgaaat aaacattgga aacctgtcgt
     1201 ccggatttgt tgccatccgt acccacatgg tgtacgagcg ggaaaatcaa ccctctgacg
     1261 acggcagaca gggtaatgaa gataccggcg tggtggacga agccggaaaa acccgtcaac
     1321 aaccacgcag cacccaaaat caaaaagtcg gcaatacccg cccacaagaa gcactgcccg
     1381 ataaagggaa tatcaacaaa gcggctgcgg caaatccgtt tgccaaaata cgcgccgacg
     1441 gcgataccgg tcagaaaaca ggcaagagta aatgaaaatg cctgaggcac ggactgtgct
     1501 gcgaacgaaa acatccttac ccacaagact tctataccca agctcaataa gccgctaagg
     1561 aaagaaagca tataaatcaa actagtattc ggtttagtgt tcactatatc catcctttct
     1621 gtaacgcagc catactgaag cagcaatcag aaggttaaag caggctgtca gcgcaatggt
     1681 ttgggagagg gtaaaaaaga cgtagaaaaa ttcggcggcg gcaagcgatc cgagtgccgc
     1741 acccaaagtg ttgaaaaaat ataaggtacc gatagactcg ccaacattat gtattttccg
     1801 gttaaaaaaa caggtcagca agggcaaggt cgcgcccatc ataaaggtag gaagcagcaa
     1861 taagaggaaa ttggcagcag cgatgatggg caaatcagcc tcaactaaaa gatgccccaa
     1921 gccggaaatc agacccctgc ttaccaaacc gaacagaccg atggatactt cagcgatgca
     1981 aaacaggggg atgatacttg aaggaaaacg gtcagcaatg cgtccaccga aatacgcacc
     2041 tacacccaag ccgaccataa atacagaaat aatgacagta atcgaactca aatcgatacc
     2101 tatgtgactg aatagaagcc tctgccagct gacctggtaa atcagggcgc agaatccaga
     2161 ggcgaaaaac accagcgaca atcctttcag ggttttactt gcggtcgaat tcatattttc
     2221 tattttcaaa taggcggaac tataaatgaa atgtatcaat gtgtaaacat ttgtattaac
     2281 cgtaggaaat caccgtattg ctcaaactgc aggccgggaa aataaactcg taagcccgca
     2341 aacggcgtat cactgttcaa actgctgtgc ggcttcacgg tgttataaaa ataatggggc
     2401 tgtcctggac aactaagata aactcgattt tactaattgt tttaaaatgg aaatt
//
LOCUS       DAWKQJ010000198         2449 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000198 DAWKQJ010000000
VERSION     DAWKQJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.198"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            25..567
                     /locus_tag="WJL20_001904"
     CDS             25..567
                     /locus_tag="WJL20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7684010.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            697..2358
                     /locus_tag="WJL20_001905"
     CDS             697..2358
                     /locus_tag="WJL20_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ7684011.1"
                     /translation="MTESITRDNMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     VTPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT          620 a          600 c          679 g          550 t
ORIGIN      
        1 cctgttgctt atggaaaaat caaagtgcag agttggaaag agaggcgcga tttcaatatt
       61 gtaaagcagg atttggattt ttcctgcggg gcggcttcgg tggcgacgct tttgaacaat
      121 ttttacgggc aaaagctgac ggaagaggac gtgttaaaaa aactggataa ggagcagatg
      181 cccgcgtcgt ttgaggatat gcggcgcatt atgcctgatt tgggttttga agcgaaaggc
      241 tatgccctgt ctttcgagca gcttgcacag ttgcaaatcc ccgtcatcgt gtatctgaaa
      301 taccgcaagg atgatcattt ctcggtattg cgcggtatag acggcaatac ggttttgctt
      361 gccgacccgt cgctgggtca tgtttcgatg agcagggcgc agtttttgga tgcttggcaa
      421 acccgtgagg gaaatttggc aggtaagatt ttggcggttg tgccgaaagg gagggatgcc
      481 ggcggggata aggcgttttt cacacgcagt ccgaaacgtc agacggagtt tgcggtcgga
      541 caggtaaaat ggtggcgtgc ttattgaaat ttcgacaaag gtcgcctgaa aaccgaaaat
      601 atggttttca gacgaccttt gttgtatttg gtaactatat gttcccgttg tataattacg
      661 gatttgcaat tcaataataa atacacagga accgccatga cagaatccat cacccgcgac
      721 aatatgcaat acgatgtcgt gattgtcggc gcaggcccgt cgggtttgtc cgccgccatc
      781 aaactcaagc agcttgccga acaaaatggg cgcgaaatca gcgtttgcgt ggtggagaaa
      841 ggttcggagg cgggtgcaca ttcgctggcg ggcgcggtta tcgatccgat tgcgctgaat
      901 gagttgattc ccgactggaa agaaaaaggc gcgccgctga cacgcacagt aacgcaggac
      961 aaggtgttgt tcctgaccga gaaaaaagcg ttcaacctgc ccgttacccc caatttcgac
     1021 aatcatggca actacatcgt cagcttgggc gaagtcgtac gctggttggc ggagcaggcg
     1081 gaaaatttgg gcgtggaaat ttatccgggc tttgccgctg ccgaagtgct gtatcacaaa
     1141 gacggttcgg tcaaaggcat tgcaaccggc aatatgggcg tgggcaaaga cggcgaaccg
     1201 accgattcat tccagcccgg catggagctt tgggcacagc aaaccctgtt tgccgaaggt
     1261 tgccgaggtt cgctttccaa gcaaatcatc gaacgcttca aactcgacca aaacagccag
     1321 ccgcaaactt acggcttggg cattaaagaa gtttgggaag tgccgtccga acagcatcag
     1381 cccggtttgg tgatgcacag cgcaggctgg ccgctggaca gcaaaaccta cggcggcgcg
     1441 tttgtttacc atttcgacga caacaaagtt gccgtcggct tcgtggtcgg tttggactat
     1501 caaaatcctt atctgtcgcc gtttgaagag ttccaacgtt tcaaaaccca tcccgaaatc
     1561 cgcaaaacct ttgaaggcgg ccgccgcatt gcttacggcg cgcgttcgct gattgaaggc
     1621 ggtttgcaaa gtttgccgaa gctctcgttc aaaggcggca ttttggtcgg cgatgcggcg
     1681 ggtttcctca atatgccgcg catcaaaggc attcataccg cgatgaaatc cgccatgctc
     1741 gccgccgaag ccgtgttccc tttgttggaa aaccttgaag aagtggaggg tttcgacagc
     1801 ggcaaagagg cggcggatta tcagcaacgt tttgaacaaa gctggctgta tcaagagctt
     1861 tacgccgcgc gcaacgtccg tccgtcattc aaatggggcg tttacctcgg ctcgctttat
     1921 accggcatcg accagatgat tttcagaggc aaagccccgt ggaccttgaa acatcacggt
     1981 aaagacaacg agcagctcaa aaaagccgcc gcgtgcaagc cgattgctta tccgaaaccc
     2041 gatggcgtgt tgaccttcga ccgcttgagc agcgtcttcc ttgccaatct cgcgcacgaa
     2101 gaaaaccagc ccgaccattt ggtgctgaac aatccgcaaa cgatgataaa cgtgaactac
     2161 aaagaatacg cctcgcccga aacgcgctat tgtccggcgg gtgtgtacga aattattgaa
     2221 gaaaacggca atccgcgcct gcaaatcaac gcggcaaact gcgtgcactg caaaacctgc
     2281 gacatcaaag acccgacgca aaacatcact tggatttgcc ccgaaggcgc gagcgggccg
     2341 aattacggcg ggatgtaaac cttattccgc cgttacgccc ggtcaggaat aaggaatggg
     2401 gcagttgttc aaacctgccc cgtttgaacc cataatatag tggattaaa
//
LOCUS       DAWKQJ010000199         2402 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000199 DAWKQJ010000000
VERSION     DAWKQJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.199"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(16..771)
                     /locus_tag="WJL20_001906"
     CDS             complement(16..771)
                     /locus_tag="WJL20_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ7684012.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(896..2052)
                     /gene="nirK"
                     /locus_tag="WJL20_001907"
                     /pseudo
     CDS             complement(896..2052)
                     /gene="nirK"
                     /locus_tag="WJL20_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
BASE COUNT          541 a          544 c          673 g          644 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aggttgtgca agacatattt
       61 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacatttgt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      781 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccactgcc
      841 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      901 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
      961 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1021 tttctgcacc ttctactttc aattgaccca gtgcgccttt gttgaatgcg cggaagatag
     1081 agtggtcaac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcagagc
     1141 cgccggcagg aacgatggtg ctttgtacgt tttcgttaat cagcttgtcg ccttcaacat
     1201 aaactttgtc gaagatttcg ccgataacgt ggaaggaaga cactaggttc ggaccgccgt
     1261 taccgacgta catacgcacg gtttcgcctg ctttggcttt cagcgcgtta tcgccggcga
     1321 tagaacctac atgaccgttg aatacgacgt attcaggctg ttcggcaacg gctttgtcca
     1381 tatcgaacgg caggccttgc gcgccttttt tgcctttggt gtagaagtcg ccttggatga
     1441 tgtagaactc tttatccact ttcggcagac cttctttagg ctcgaccaaa atcagaccgt
     1501 acataccgtt ggcgatgtgc ataccgaccg gcgcgacggt gcagtggtag atgtacagac
     1561 ccggttgcag ggctttgaag ctgaatgtgg aagtgcgacc cggggcggta aaggttgcgg
     1621 ctgcaccgcc gccctgaccg gttgcggcgt ggaagtcgac gttatgggga acggtagaag
     1681 acgggttgtt ggaaaattcc acttcaaccg tatcgctttc gcgtacgcgg atcatgcggc
     1741 ccggaacgtc gccgtcaaat gtccagtagc ggtattccac accgtcttcc atggtcatgg
     1801 tttttcgaca gtttccattt ttacgcggac tttggcagga tagtcgcggt cgattgcggg
     1861 aggtacttca ggagcgtggg tggtaactgc atcgataacg ggcagttcgc ctgtcggcgt
     1921 ttcggcggcg gtttgtgcgg cggaacttgc ggcttctgcg gaagcggcag gggcttgcgc
     1981 ggcaggttcg cctccgcagg cggctaatgc gaataaggaa gcaatcattg cagctaaggc
     2041 ttggcgtttc ataatgtttt ccttttgtaa gaaaagtaga ggaattggtt aagaacttgg
     2101 tgtgattatg cgtttgcccc attgggggca ttttgattta agtcaaataa ttcataaaat
     2161 atgaacttaa aatgatactt ctaatacttt atagctacta aaaattacaa tgaataggct
     2221 gatttgctta agggcttcgg caaatcaggc aaaggcaggg tagttgccga ctaaagaata
     2281 atgatagtta ttatcatata tgtatttgtt ttatatgtga gtttctttga acaaatgttt
     2341 atttgtgcgg taaaccgtgc tacaatcttt aacattcata ttctgtgaat tttaaacagc
     2401 tg
//
LOCUS       DAWKQJ010000200         2339 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000200 DAWKQJ010000000
VERSION     DAWKQJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.200"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..2257
                     /locus_tag="WJL20_001908"
     CDS             44..2257
                     /locus_tag="WJL20_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7684013.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          603 a          758 c          564 g          414 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgattg gaacctattg gtttccgaac gctgcaacag
      361 taccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacgc cggaagaacg cgcccagcag gcagaaacta tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt tgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga gcccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgtaccg aacgcctcga atacatcgaa aacttcctta atatcctgcg
      781 tacggaactc aaaaaataca atatccactt tgaagtcgcc ggccgtccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacgggttgt tcgacatccg
      901 cgccgtgcgg attctggttg ataccgtccc cgagtgttac accacactgg gcattgtcca
      961 cagcctctgg cagcccattc ccggcgagtt cgacgactac atcgccaacc cgaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgcgc actggcgtta
     1141 caaagagggc ggcaaaggcg attccgccta cgaacaaaaa atcgcctggt tacgccaact
     1201 tttggactgg cgcgaaaaca tggcggaaag cggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt tttgaccccg cacggcaaag tcctctccct
     1321 gcccacaggc gcgaccccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgtgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctttacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgcccg tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacaagcacc
     1981 tgaaaaagtg ctggacgcaa gctgggcggc gttgcaggaa ggacaagtgt tcgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcactcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagcat
     2161 gaggttcacg ctcgaagtca aacaagtcaa cgacctcccg cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgtataacgc ttcagacggc attttgattg acggggtttg ctattttttg ttgcataat
//
LOCUS       DAWKQJ010000201         2328 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000201 DAWKQJ010000000
VERSION     DAWKQJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.201"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(64..1473)
                     /gene="dacB"
                     /locus_tag="WJL20_001909"
     CDS             complement(64..1473)
                     /gene="dacB"
                     /locus_tag="WJL20_001909"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ7684014.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1638..2204
                     /locus_tag="WJL20_001910"
     CDS             1638..2204
                     /locus_tag="WJL20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7684015.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          533 a          570 c          670 g          555 t
ORIGIN      
        1 aatcgagttt atcctagtta tctaggacag ccccttaaat ttaatccact atattttcct
       61 gtttcaggct cggcgttctt tgcacatcag tttcgcatcc agccagccat cgccgccgga
      121 gatgatgttg ttggcaacga agttgtccaa gtcgggcagc agggaaacgg cgcggccgct
      181 gttgatgatg acgaccaccg ccatcggttt gtcgcccagc caataacctg caagggcgcg
      241 gacattgttg agcgtgccgg tttttaagcg caacagcccg ccgctttgtt tgaagcggtt
      301 gcgtaaagtc ccgtctgtgc cggcgatggg cagcgtgtcg atgaaatctt gtgcaaacgg
      361 gctgaaataa gccgtttcca acatttgcgc catcattctc gccgttaccc tttcttttct
      421 agacagaccc gaaccgtttt ccaaaaccaa atccgcaaca tcgatgcccg acacggcaag
      481 ttcgcgccgg acggcagacg ctgcctgttc ggaaacggcg ggcagtttgc cgtcgccgcc
      541 gagtttgagg aagacggaac gcgcaattag attgtccgaa cgcttgttca tgtccgtcaa
      601 aatttccttc atcggtttgg cgtgtgcaac ggcaagcgtc tgcgcgcctt ccggcgtgtc
      661 ggctatgccg ataccgtctg aaatccgtcc gccgccgagc agccagtggt tggtaaaact
      721 ttgccggatc agttcgtcaa gcgcgaacat ccggacaccg acaggcttgc ccaaacagct
      781 ctcgggaata ttgccgcgca atttcagcgt attgtccgaa aaagatgcac gcatcagttt
      841 tttgatcgaa gggcaggcag cttgggaggc ggtaatttgc aagttgtttt gggcgaaaat
      901 atgcggcaaa ggcggatcgg tgaggatgtc ggtactgtcg gcggcattgc gttcggcgcg
      961 caccataacc ataccggcag acagcatagt tggattgggg ggcgtcataa acggcgaacc
     1021 gctgtcggct tcgaaatcgt cggggctgcc gacttcgccc cacaggctgt ggtcgagcat
     1081 caggtgtccc gtgatattgc gtatgccttg ttcgcgcaac tggcgttgga cggcaagcag
     1141 gttttcctga ttgaaaacgg ggtcgccgct gcccgcccaa tacaggtttc cgtcaagcgt
     1201 gccgtcgttt accgtaccgt tgcttttaaa ctcggtcgcc cagcggtaat tgctgccgaa
     1261 ggttttgaag gcggcaaacg cggtaacgag tttcattgtg gaggcggggt tgacggggac
     1321 atccgagcgg tggtcaatga tgacttttcc gctgtcaagc tcttggacat atacggcgat
     1381 ttcgttttgc ggaatgcggc cggtatcgag cgcgtgtgcg gcgagggagg cgagaagcag
     1441 cagcagggag gccgctgttt tggggaaatt cataggtaaa tctgttacat aaaaaaagca
     1501 attataaggc aaagccggat aagtgggaac gctgtggcgg cgggccggct tgtttgcagg
     1561 ggaaatcaca tatataataa tcgctatcat tatgaaatga ttgagacgca caaacttaat
     1621 attcaggagg aatgattatg gctaagaaaa tcagtatttt ggtgggcagc ctgcgccgag
     1681 cttcttttgc gcgcaaagtg gcattgaatg cggcggaaat gttccccgaa ggctggcagg
     1741 cggaaatcgt cgaaatcgga catctgccgc tttacaattt cgattatgac gaccctgcgg
     1801 tggaagatgt gccgctgccc gaaagctaca cggctttccg cgaaacgatt aaggcttcgg
     1861 acggcatttt gttcgttacg tccgaaaaca accgcaccat tcccgcctgt ttgaaaaatg
     1921 cggtggacat cggctcgaaa ccgaatgccg acgtggcgtg gaaaaacaaa ccggccggta
     1981 tcatcagcca ttccgtcggc aagatgggcg gttacagttc gcaaaaaaac ctgcgccttg
     2041 cgctgtctta tttcgatatg cccgtaaccg gacagccgga agtgtttttg ggcaattcgc
     2101 cgacactgtt tgatgaaaac ggcaaattga tcgactcggc aagggatttt gttcagtcat
     2161 atattaatca gtttgtcggt ttgattgaaa gaaatgccaa ataaacaaac caagtcgaaa
     2221 ggccgcaaaa ccggttcggg ttttgcggct tttttatatg ggctgggcag cgtcaaggtt
     2281 tgccgtttgc cggataatgc gcggcaaatg accggcgtgt ttatagcg
//
LOCUS       DAWKQJ010000202         2326 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000202 DAWKQJ010000000
VERSION     DAWKQJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.202"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..512
                     /locus_tag="WJL20_001911"
     CDS             33..512
                     /locus_tag="WJL20_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ7684016.1"
                     /translation="MPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGFDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(599..1567)
                     /gene="ispH"
                     /locus_tag="WJL20_001912"
                     /gene_synonym="lytB"
     CDS             complement(599..1567)
                     /gene="ispH"
                     /locus_tag="WJL20_001912"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ7684017.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAEERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1596..2093)
                     /locus_tag="WJL20_001913"
     CDS             complement(1596..2093)
                     /locus_tag="WJL20_001913"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ7684018.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKH"
BASE COUNT          529 a          720 c          569 g          508 t
ORIGIN      
        1 gcctgcccga aatcatccgc gacctgctcg aaatgccgtc tgaaaccgcc gttgccgaca
       61 tcgcgcgcac ttattccgcg cattacctca atcccaacaa ccgcaatatg acgctctttc
      121 ccgatgccct gccctgtctg gacaagctca aagcacaagg atactggctt gccgtcgcca
      181 cgggcaaagg gcgggcgggt ttggacaacg ccatcagcca aaccgccacc ggcggctatt
      241 ggctcgccac cgcctgcgcg ggggaatatc cgtccaaacc ctcgcccgaa atggtattcg
      301 gactctgcgg cgaactgggg ttcgacccga aagaggcatt ggtcgtcggc gatacggcgc
      361 acgacctgca tatggcggca aacgcaggcg cggcggcagt cggcgtggcc accggcgcac
      421 attcgcgcga acagctccgc accgcaccgc atctcgccgt attggacggt ttgtccgaac
      481 tgcccggttt tcttgcacag cattacgcct gattggtttc cgcatcagac actcaaaaca
      541 aatgccgtct gaagcctgtt cagacggcat ttgtgttgcc caaacattca acgcttgctc
      601 aacgtttgca caaatcgggt ttggtttcgc cctcgcggcg cagctctttg ggcaggacga
      661 acacaatgct ttcttccgca ccctcgcctt cgcgtacggt ttcgtgcccc catccgcgta
      721 tggttgccag tacttcccgc accaacactt cgggcgcgga cgcgcctgcc gttacgccga
      781 ctttgttttt gccctcaaac catgcacgtt gcaggtagcc tgcattatcc accatatacg
      841 catcgattcc gcgcgatgcc gccacttcgc gcaagcggtt gctgttggac gaattgggcg
      901 aaccgaccac aatcacgatg tcgcactgtt ccgccaactc tttgacggcg gtttgccggt
      961 tggtcgtcgc atagcagata tcttccttgt gcggattgcg gatattgggg aaacgcgcgt
     1021 tcagcgcggc gatgatgtct ttggtttcat cgaccgagag cgtggtttgg ctgacatagg
     1081 cgagtttgtc ggggtttctg acttcgagtt ttgccacatc tccgaccgtt tcgaccaaaa
     1141 gcattttgcc cggcgcaagc tgccccatcg tgccttcgac ctcgacgtgc cccttatgcc
     1201 cgatcatgat gatttcacag tcttgggcat ccagtcgggc gacttcctta tgcactttcg
     1261 tcaccagcgg gcaagtcgca tcaaacacgc ggaaaccacg ttcctccgct tcttgctgca
     1321 ccgccttcga tacgccgtgt gccgaataaa ccagtgtcgc gcccggcggc acttccgcca
     1381 agtcttcaat aaacaccgca cctttttcac gcaggttgtc cacgacgaat ttgttgtgaa
     1441 cgacttcgtg gcgcacataa atcggcgcgc cgaactcttc caaagcacgt tcgacaatac
     1501 tgattgcccg atccacacca gcgcagaagc cgcgcggatt ggcaaggatg atggttttct
     1561 cgttcataag cccggtattt cgttttcaga cggcatcaat gtttttcttc ttgggtttta
     1621 cggtggacga tgttgtccaa cactgccaac accgcaccga cgcagataaa gctgtcggca
     1681 atattaaagg cgggataaaa ccaattttgc caataaaaca ataagaaatc gacgacatga
     1741 ccgtgtatca ggcggtcgat gacattgcct aacgcaccgc cgataatcat cgccgcaccc
     1801 gttttgccga gggttgcaaa ctcatcgcgc aagatggcgc gtaccaaata cgcgctcacc
     1861 gccaccgcca gcaccaaaaa aaagtatttt tgccagccgc cctgatcggc aaggaagctg
     1921 aacgccgcgc ccgggttgta caccaaggtc agatcgaaaa acgaaggaat gacattgacg
     1981 cgttcccgat actgaaacga cgacagcacc gcccacttcg accactggtc cagcacgatg
     2041 gcggcaagtg ccaataccca atagcgcgtt ttacttgaaa cagatgaaga catatttttc
     2101 aacagccggt aaaagagtac cattttaccc gaaaaccccc tttcctgtac ccgaaacggc
     2161 aaatgccgta atcttaaaac ccgtcattcc cgacaacacc gtaatctcga aacccgtcat
     2221 tcccgcgtag gcgggaatcc agacctgtcc gcacagaaac ttatcggata aaaacagttg
     2281 cccaaacccc gcgttctata gtggattaaa ttcaaaccag tacggc
//
LOCUS       DAWKQJ010000203         2326 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000203 DAWKQJ010000000
VERSION     DAWKQJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.203"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..329
                     /locus_tag="WJL20_001914"
     CDS             <1..329
                     /locus_tag="WJL20_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ7684019.1"
                     /translation="IATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSSFVNIGGQWYFIDPTVPLPTMKQPCICGSEKKFKACCGK
                     YLQPVA"
     gene            complement(546..1340)
                     /locus_tag="WJL20_001915"
     CDS             complement(546..1340)
                     /locus_tag="WJL20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ7684020.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTVRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1444..2169
                     /locus_tag="WJL20_001916"
     CDS             1444..2169
                     /locus_tag="WJL20_001916"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ7684021.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFNGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          625 a          749 c          504 g          448 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaaggg ctgggcttga acgtcatctc gcacaagata ctcggcaaag
      121 accacgccca agtcgaattt gaagcctact tccgagacgg acaacaccga tccgcacatc
      181 acgaactgtc cagcttcgtc aacatcggcg gacaatggta tttcatcgat ccaactgttc
      241 cgctgcctac catgaaacag ccctgcattt gcggttctga aaagaaattc aaagcctgct
      301 gcggcaaata tctgcaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 caacgcgcaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg acagcgttaa ccagcaaaag
      481 cattgtacct ttattaatgt taaaaaacat taattttgaa aatatattaa caaacctgtt
      541 gaaacctatt ggttttcccg tatccacccg acccagcgtt caaacagctt cggttcgagc
      601 gcggcaacga ccgagcgttt gaacacgtgt tcaccactcc aaaatccgtc gccttccaaa
      661 gtcgtcagcc tgccgcccgc ctcctcaaaa atcagcgcac cggcggcata atcccatagc
      721 ttctgcccgc cgtggacata aacatcataa cgcccgcacg ccagataaca ccagtccagc
      781 gtgctgctgc ccatactccg caccgtaccg aaaggcgcga gcgtgctcat ccggctggac
      841 agcttgccgg aacgcagata tttgatttcc acacccgcga ttgcctcatt caacttctta
      901 tccacgaggc gcaaaggcag gcgggttcca ttaagaaacg caccctgccc ccgttcggca
      961 taaaaacatt cgccgctgac cggattgtaa atcacaccca attcggcgcg cccgttgcgg
     1021 acaaacgcca ccgacacggc aaaatgcggc agcccgttga caaaattgtt cgtcccgtct
     1081 atcggatcga caatccacag ccccttttcc cccgaatatt gttcccacaa agccgactgt
     1141 tcctgccgcg acatttcctc gcccaacatc ggactgtcga ttaaaagcgg caacgcggcg
     1201 gcaaaagccg tctgcgcggc aatgtccgcc tcgctcaaca tcgaaccgtc ttccttgcgg
     1261 caagacggcg tattcaaaaa acgcggcata atttcggttt gcgcgatatg gcgcacgact
     1321 ttctgcaaac ggtgcaacac ttcctactgt cctcatattt tgaacttgcg gcgcgcaaac
     1381 gtataatgtc cgtttccatc acgccgctgc gacggattat aaccgtccga accgccaaaa
     1441 actatgcccc gattctacct gcccgaaaac ctttccgtcg gacaaaccgt cgccctgccc
     1501 gacaacatcg tccgccacct caacgtcctg cgcgtccgcc ccaacgaaaa catcaccctc
     1561 ttcaacggca aaggcaaggc acacgccgca cagctgaccg ttttggaaaa acgccgcgcc
     1621 gaagccgaaa tcctgcacga agacacaacc gacaacgagt ccccgctcaa catcacgctc
     1681 atccaatcca tatcttccgg cgaacgtatg gactttaccc tgcaaaaaag cgtcgaactc
     1741 ggcgtaaccg ccatacagcc cgtcatcagc gaacgctgca tcgtccgcct cgacggagaa
     1801 cgcgccgcca aacgcctcgc acgctggcag gaaatcgtca tctccgcgtg cgaacaaagc
     1861 ggcaggaaca ccgttccccc cgtgctgccc atcatcggct atcgtgaagc actcgacaaa
     1921 atgccgtctg aaagcaccaa gctgattatg agcatcaacc gcgcccgcaa actcggcgac
     1981 atccgccacc cgtccggcgc aatcgtcttt atggtcgggc ccgaaggcgg ctggacagaa
     2041 caggaagaac aacaggcatt tgaagccggc tttcaggcgg ttacactcgg caaacggatt
     2101 ttacgcacag aaaccgcccc actcgccgcc ctcgccgcca tgcagacgct ttggggcgat
     2161 ttcgcataaa cagaaatgcc gtctgaaacc cgttcagacg gcattttgca gccgattaag
     2221 atagtaggtt caaataagat ttcccccgcc gtcattcccg cgaaagcaag aatctagaaa
     2281 cgaaaaacta cagggattta tccgaaacaa caaaccctct ccgccg
//
LOCUS       DAWKQJ010000204         2262 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000204 DAWKQJ010000000
VERSION     DAWKQJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.204"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..567)
                     /locus_tag="WJL20_001917"
     CDS             complement(<1..567)
                     /locus_tag="WJL20_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ7684022.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            731..2137
                     /gene="dnaB"
                     /locus_tag="WJL20_001918"
     CDS             731..2137
                     /gene="dnaB"
                     /locus_tag="WJL20_001918"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ7684023.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          543 a          572 c          617 g          530 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccaaaaacct ttcaggtagt tgggacggct
       61 gttgcggtag tcgatgtaat aggcgtgttc ccatacgtcg caagtcaaga gcggcgtgtt
      121 ttcagtggtc agcggcgtag cggcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggtttttacc agccacgccc aaccggagcc gaaagtaccg gcagcgcaag cggagaatgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgata gcggcggcca gttcgccggc
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaagtgtggt tccaagtttg
      361 ggctgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggtaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tttctttgct cagatgcggg gacaatgcgt ccggttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tgttgttttt ggatgttgtg
      601 ttttggcagt gttgctgcaa acgaatcggc agcacacgca ttctacctgt tttgagggat
      661 ggaaaccaat tgaacctgct ttacgctata atagaagatt gcaatttcgg cacgacagat
      721 aggatgtacc atgaacgatt acgccgctat gccgtctgaa gaccgtgagg tcggcgcatt
      781 gtcgctgcct ccacattcga tggaggcaga acaatccgtt ttgggcgggt tgatgctgga
      841 aaatccggct tgggacagga ttgccgatgt ggtttcggga gaggatttct accgccacga
      901 acaccgcctg attttccgat ccattgccaa attaatcaat gagagccgtc ccgccgatgt
      961 gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag gcggattcga
     1021 atatctgatt acgctggcgc aaaacacccc gtctgccgcc aacatccgcc gctacgccga
     1081 aatcgtgcgc gagcgttcca ttatgcgcca actcgccgaa gtggggacgg aaatcgcccg
     1141 cagcgcctac aatccgcaag gcagggacgc ggggcagctt ttggacgagg cggaaaacaa
     1201 agtattccaa atcgccgaaa gcacggccaa atccaagcag ggctttttgg agatgcccga
     1261 tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca atcccgatga
     1321 agttaccggc gtgccgacag ggtttatcga cctcgacaaa aaaacctcgg gtctgcagcc
     1381 cggcgacctg attatcgttg ccggccgtcc gtctatgggt aagaccgcct tttcgatcaa
     1441 tattgcggaa cacgttgccg tagaaggcaa gctgcccgtt gccgtctttt cgatggagat
     1501 gggcggtgcg caactggtca tgcgtatgct cggctcggtc ggacggttgg atcaaagcgt
     1561 cctgaaaaca ggcaggttgg aagacgaaca ttggggtcgt ctgaatgaag cagtcgtcaa
     1621 actctcagac gcgcccgtgt acatcgacga gaccccgggt ctgactgcgc tcgaactgcg
     1681 cgcccgcgcc cgtcgtctcg cccgtcaatt taacaataag ctgggattaa tcgtcatcga
     1741 ctacctgcaa ctgatggcgg gatccggccg ttccgacaac cgagcttcag agctgggcga
     1801 aatttcacgt tcgctcaaag cgttggcgaa agaattgcaa gtccccatca tcgccctgtc
     1861 gcaactgagc cgcacggtcg aatcgcgtac cgacaaacgc ccgatgatgt ccgaccttcg
     1921 cgaatccggt gctatcgagc aggatgccga cctgattatg tttatgtacc gcgacgaata
     1981 ctacaaccaa gactcgccca tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa
     2041 cggtcccgtc ggtaaaatct tcctcacatg gacggggcaa ttcaccaaat tcgacaatgc
     2101 tgcctatatt cccgaggagg caaagataga agattaaatg gctatataaa aatttattag
     2161 gcgaaatcag gcaaaatcgt ttaaaatcat gctgagagat tgccctaaaa aataaaacgc
     2221 ggtcttgagg catttttgca ttcagcccgc atataattga aa
//
LOCUS       DAWKQJ010000205         2216 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000205 DAWKQJ010000000
VERSION     DAWKQJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.205"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WJL20_001919"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WJL20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ7684024.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          564 a          631 c          578 g          443 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttatgct tccggtctgt
      601 ccggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgaatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccatgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggtcgta
     1621 tgttcgtatc acccaacgac aaaatctacg aaggtatgat tatcggcatc cacagccgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgcgccagcg
     1741 gcaccgacga agccgtgcgc ctgaccacgc cgatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccttttga aggaatacgc cttttgagtc cttacaagct
     2161 taggaagagg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcaga
//
LOCUS       DAWKQJ010000206         2164 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000206 DAWKQJ010000000
VERSION     DAWKQJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.206"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            164..1549
                     /locus_tag="WJL20_001920"
     CDS             164..1549
                     /locus_tag="WJL20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ7684025.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTTAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1569..2087
                     /locus_tag="WJL20_001921"
     CDS             1569..2087
                     /locus_tag="WJL20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ7684026.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          458 a          655 c          580 g          471 t
ORIGIN      
        1 aggcaacgcc gtactggttt ttgttaatcc actataaatc gttcaaataa acaggaatat
       61 aacttcagac aaacaactta ccgcccgatt tgtgctatcg ttttcgcaca acttaaaaaa
      121 acctgacaat tttgtacttt tattacagag aaaggcttta caaatggacg gctggacaca
      181 gacgctgtcc gcgcaaaccc tgttgggcat ttcggcggcg gcaatcatcc tcattctgat
      241 tttaatcgtc aaattccgca tccacgcgct gctgacactg gtcatcgtca gcctgctgac
      301 ggctttggca accggtttgc ccacaggcag cattgtcaac gacgtactgg tcaaaaactt
      361 cggcggcacg ctcggcggcg tggcgcttct ggtcggcctg ggcgcgatgc tcggacgtct
      421 ggtcgaaaca tccggcggcg cacagtcgct ggcggacgcg ctgatccgga tgttcggcga
      481 aaaacgcgca ccgttcgcgc tgggcgttgc ctcgctgatt ttcggcttcc cgattttctt
      541 cgatgccgga ctaatcgtca tgctgcccat cgtgttcgcc accgcacggc gcatgaaaca
      601 ggacgtactg cccttcgcgc ttgcctccat cggcgcattt tccgtcatgc acgtcttcct
      661 gccgccccat ccgggcccga ttgccgcttc cgaattttac ggcgcgaaca tcggccaagt
      721 tttgattttg ggtctgccga ccgccttcat cacatggtat ttcagcggct atatgctcgg
      781 caaagtgttg gggcgcacca tccatgttcc cgttcccgaa ctgctcagcg gcggcacgca
      841 agacaacgac ctgccgaaag aacctgccaa agcaggaacg gtcgtcgcca tcatgctgat
      901 tcccatgctg ctgattttcc tgaataccgg cgtatcggcc ctcatcagcg aaaaactcgt
      961 aagtgcggac gaaacctggg ttcagacggc aaaaataatc ggttcgacac cgatcgccct
     1021 tctgatttcc gtattggtcg cactgtttgt cttgggacgc aaacgcggcg aaagcggcag
     1081 cgcgttggaa aaaaccgtgg acggcgcact cgcccccgtc tgttccgtga ttctgattac
     1141 cggcgcgggc ggtatgttcg gcggcgtttt gcgcgcttcc ggcatcggca aggcactcgc
     1201 cgacagcatg gcggatttgg gcattcccgt ccttttgggc tgtttccttg tcgccttggc
     1261 actgcgtatc gcgcaaggtt cggcaaccgt cgccctgacc accaccgccg cgctgatggc
     1321 tcctgccgtt gccgccgccg gctttaccga ctggcagctc gcctgtatcg tattggcaac
     1381 ggcggcaggt tcggtcggtt gcagccactt caacgactcc ggcttctggc tggtcggccg
     1441 cctcttggac atggacgtac cgaccacgct gaaaacctgg acggtcaacc aaaccctcat
     1501 cgcactcatc ggctttgcct tgtccgcact gctgttcgcc atcgtctgac agacggaaag
     1561 gatagtaaat gactacgcat tttgtcgtta tgggcgtatg cggctgcggc aagaccaccg
     1621 ccgcgctgtc cctgcagaaa cacctcggtc aatgtcccta tgccgaaggc gacgagttcc
     1681 acacccaagc caaccgcgac aagatgggcg cgggtattcc gctgaccgat gaagaccgct
     1741 atccgtggtt gggcaatctg cgcgactgga tgacgaaaca ggcgcaaaac ggtgcgaacc
     1801 acaccatcgt aacctgttcc gccctcaaac gcggctaccg cgacattctg cgcggagccg
     1861 aaggcaaagc tgccttcatc cacctcagtc cgccgcaaga catcaacctc gagcgcatga
     1921 tgtcgcgcaa aggacattat atgaaagcag ggatgctcga ttcgcaactg gaaatcctcg
     1981 aggaactggg cgaaggcgaa tacggggtca aaatcgccaa ccccggcacg cccgaagcgg
     2041 tcgaagccga tattctgaac tgggttgcct cggaaaacct gctttgaagc aatatgccgt
     2101 ctgaagcccg acacaggatg ggtttcagac ggcataaaca tcgggaacag aatggattac
     2161 attg
//
LOCUS       DAWKQJ010000207         2148 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000207 DAWKQJ010000000
VERSION     DAWKQJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.207"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            373..885
                     /locus_tag="WJL20_001922"
     CDS             373..885
                     /locus_tag="WJL20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ7684027.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="WJL20_001923"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="WJL20_001923"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ7684028.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          526 a          672 c          523 g          427 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgcagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaaaccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cattctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgt tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgccggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcggttt cggtcgtt
//
LOCUS       DAWKQJ010000208         2078 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000208 DAWKQJ010000000
VERSION     DAWKQJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.208"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            234..2012
                     /locus_tag="WJL20_001924"
     CDS             234..2012
                     /locus_tag="WJL20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ7684029.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDMLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDDKGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          637 a          507 c          526 g          408 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgccgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggcg aatgctaccg atgaagatga agaagaagag ttagaatccg
      421 tacaacgctc tgtcgtaggg agcattcaag ccagtatgga aggcagcggc gaattggaaa
      481 cgatatcatt atcaatgact aacgacagca aggaatttgt agacccatac atagtagtta
      541 ccctcaaagc cggcgacaac ctgaaaatca aacaaaacac caatgaaaac accaatgcca
      601 gtagcttcac ctactcgctg aaaaaagacc tcacaggcct gatcaatgtt gaaactgaaa
      661 aattatcgtt tggcgcaaac ggcaagaaag tcaacatcat aagcgacacc aaaggcttga
      721 atttcgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtatcg
      781 gttcgacttt gaccgatatg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgcaggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag gcaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgacaa gggcgcgttg aatgtcggca gcaaggatgc caacaaaccc gtccgcatta
     1681 ccaatgtcgc cccgggcgtt aaagaggggg atgttacaaa cgtcgcacaa cttaaaggcg
     1741 tggcgcaaaa cttgaacaac cgcatcgaca atgtggacgg caacgcgcgt gcgggcatcg
     1801 cccaagcgat tgcaaccgca ggtctggttc aggcgtatct gcccggcaag agtatgatgg
     1861 cgatcggcgg cggcacttat cgcggcgaag ccggttacgc catcggctac tccagtattt
     1921 ccgacggcgg aaattggatt atcaaaggca cggcttccgg caattcgcgc ggccatttcg
     1981 gtgcttccgc atctgtcggt tatcagtggt aagggctttt tcgcctgtct gctgttggga
     2041 caggcggaag gtttgaaggg aagggtggcg atttgccg
//
LOCUS       DAWKQJ010000209         1999 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000209 DAWKQJ010000000
VERSION     DAWKQJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.209"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            299..1756
                     /locus_tag="WJL20_001925"
     CDS             299..1756
                     /locus_tag="WJL20_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7684030.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          453 a          594 c          457 g          495 t
ORIGIN      
        1 tgcgcccttc gacgtggcgg atatataagg aagtgatttt ccatctaagt aaaaaccgcc
       61 ctatcggata agcccttaac agaaaaggct ttacccgcgc cgtatcggaa cacatcctct
      121 aaaatacaat ccgttgaatt gaaaaaaata taaaaacatc cgcccgcgaa aaacggcagc
      181 gcgtcgtttg acaaagattg aaaatatcgg ttaaaaaccg attttcatac aaaaaacacc
      241 gctgccgtcc gcatccgttt cagacggtat tgagagaaaa tcttttagga gaacctttat
      301 gtcccggcat cccgccccca ccggagaaaa aacattcttc ggccacccct tccagctttc
      361 caccctcttc catatcgaat tgtgggaacg tttttcattt tacggaatgc agggcatcct
      421 gctgatttac ctctactaca ccgccgacaa aggcggcttg ggcatagaca aaaccctcgc
      481 cggcggcatt gtcggcgcat acagcggcag cgtgtacctg tccaccattt tgggggcgtg
      541 gtttgccgac cgagtatggg gtgcggaaaa aaccctcttc ctctcgggca tcgtcgtgat
      601 gctcggacac atcgtccttg ccgccgcccc gggcctgtac ggccttttaa tcgggctgat
      661 attcatcgca ttgggcagcg gcggcgtgaa atctacggcc agttctatgg tgggcgcatt
      721 atacgaacaa gacgaaatgc gcccgctgcg cgatgcggga ttttccattt tctacatcgc
      781 catcaatatc ggcggcttcc taggcccgct gctgaccggc ctgcttcaag aaaacatcgg
      841 tttccattat ggtttcggcg cggcagcggt cggtatggca ttcggcttgt ggcgttattc
      901 cttgggacgt aaaaacctgc cccaccccac cgtcccccat ccgctttcaa aaggacaggg
      961 caaaactgcg gccgccgtcg gcattgctct catcgccgca cttgcaaccg ccatcaaaac
     1021 ggggcttgtc aacctcgaca atttctccgg catcctctta tctaccgtca tccttgccgt
     1081 cattgcctat ttcgcccgcc tgctgaccaa cccccgcgtc agttccgaca acaagcggca
     1141 catcatcgcc tacatcccgc ttttcctgac catctgtatg ttttgggccg tctggtttca
     1201 gatttacacc gtagcaaccg tctatttcga cgaaaccgtc aaccgcacca tcggttcgtt
     1261 taccgtgccc gtcgcctgga aagattccat gcaaagcctg tgggtcatcc tgttttccgg
     1321 actgatggcg gcaatgtgga caaaaatggg gcgcaaacag cccaaaaccc cgctgaaatt
     1381 cgctatggcg gtatttgtta ccggcgcgtc gtttttggga ttcgtcccct ttatttccgc
     1441 cggcacgccg atgcctattg cggttttcgc actgattgtc cttgccatca cgataggcga
     1501 actgatgatt tccccgattg cgctgtccat ctccaccaaa atcgcaccgc ctttattcaa
     1561 aacccaaatg gtcgccctta atttccttgc cttttcatta ggcttcactt tgggcggcgt
     1621 attgtttgaa aaaggctatc aggcgggcga cgaaatcggc ttctatcggc tgctgttcta
     1681 catcggcgca gccacaggct tcctgctgct cctgctcgtc cccaaattga acaaaatgct
     1741 cgaaggcaca gactaagtcc cgccccgatg ccgtctgaac ccttcagacg gcatttttcc
     1801 gcataatgaa accaaaccgt ttccacccga caggacaggc tccccccaac cggaaggcag
     1861 cctgccgatt gtcatttaaa tatctgactg attccgtctg tttgcaaggg aaatagccgt
     1921 ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat ccgactaaat
     1981 tagtgctgca tcaacggaa
//
LOCUS       DAWKQJ010000210         1894 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000210 DAWKQJ010000000
VERSION     DAWKQJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.210"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(205..1023)
                     /gene="cysE"
                     /locus_tag="WJL20_001926"
     CDS             complement(205..1023)
                     /gene="cysE"
                     /locus_tag="WJL20_001926"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ7684031.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1207..1785
                     /gene="grpE"
                     /locus_tag="WJL20_001927"
     CDS             1207..1785
                     /gene="grpE"
                     /locus_tag="WJL20_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ7684032.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
BASE COUNT          434 a          432 c          579 g          449 t
ORIGIN      
        1 cgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tatttccggt
       61 tgcgtgggaa tcgggtgtat tgaataaaag gcattttgtc cgactggcaa gtgccgacat
      121 cggcggcata tcaaggcgca ggcttgaagc gggcaatgtc gtctgaagcc cgtttggcgt
      181 ttcagacggc attggtgcgg atattcaaat cataaagtcg atttcggtaa actggatatt
      241 ttgatccata tccgccgacg gtgttttgag cgatcgcgcc acgggtttgg cgggtacgcc
      301 gacaaccgtg atggacggcg gcacgtctga aaccacgacg ctgcccgccc cgattttggc
      361 attgctgccg atgcggatat tgcccaatat cgaggcgttt gcgccgatca tcacgccgtc
      421 gccgatttta gggtggcggt cgccgccttc tttgcccgaa ccgccgagcg ttacgccgtg
      481 caaaatcgaa atattgttgc ccaacacggc ggtttcgccg gcaacaaagc cggtggcgtg
      541 gtcgagcatc agcccgtatc cgaaacgggc ggcgggatgg atgtccacgc cgaatacttc
      601 ggacatacgg ttttgcagga aatacgccag cgttttgcgc ccgtcgagat acagccgatg
      661 gttgatgcgg tgtgcctgaa tcgcgtggaa gcctttgaaa tataaaagcg gcagcgaata
      721 ttcgtcgcag gcgggatcgc gttcgtagat ggcttttaag tctgcttcga cgcatttgcc
      781 gatttgggtg tcgctgccca acgcctgctg gtagatttca aacagcgcgc gcacgtccat
      841 aatcgggctg ccgagtttgc tggaaaggtg gtaggcaagg acggagccga gggactcgtg
      901 gcgcaacacg gtttggtgca aaaaacttgc cagcatcggt tcggcggaga ccgcggccgc
      961 ggtttcttcg cggatggtgt gccagaggtc gaaaccggtt gtgtttaaat ggtctttttt
     1021 catgagtgat gacgtttgaa aatcgatatg gtcggcagta tcttaccgtc tatattattt
     1081 tttcggtagg ggatttgaaa atgaatttga aattctctgc ttttgcttga agtttcttga
     1141 aaatgtcctt atcttgcgcg ggtaataact ggattttgat ttccaatttg ttttaaggga
     1201 tacgatatga gcgaacagac acagcagcaa aacagtgaag aagcggttga aaatgtggag
     1261 gcggtggaaa ccgtcgagac agtaggaaat gcggacggtg tgcaggaaca ggctgccgca
     1321 gagccggctt atgaggattt gcaggcgcgg attgccgagc tggaagcgca gttgaaagac
     1381 gagcagctgc gcgctttggc aaacgagcaa aacctgcgcc gccgccacca gcaggaaatt
     1441 gcggatacgc acaagttcgc cggacagaag tttgccgtgg aaatgctgcc ggtcaaggat
     1501 tatctggaaa tggcgctttt ggatcagagc ggcaatttcg atgcgctgaa aatgggcgtg
     1561 cagatgactt tgaacgagtt gcagaaagca tttgatgcta cgcaaatcaa ggaaatcaac
     1621 cctaaagcgg gcgataagct cgatccgaat atccatcagg cgatgcaggc ggtggcaagc
     1681 gaacaggagc cgaataccgt ggtgggtgtg atgaagaagg gttatacgct gtccgaccgc
     1741 gtgttgcgcc cggctatggt tacggtggcg cagaaggaag cctgaaggcg tctggggaat
     1801 aatctgattt atttcctgaa gcgcgttttg cgtataaacc gatcgaagta aagcggcaat
     1861 gccgtctgaa cccgcctgtc gggcttcaga cggc
//
LOCUS       DAWKQJ010000211         1848 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000211 DAWKQJ010000000
VERSION     DAWKQJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.211"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            79..1248
                     /gene="metZ"
                     /locus_tag="WJL20_001928"
     CDS             79..1248
                     /gene="metZ"
                     /locus_tag="WJL20_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ7684033.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1343..1549
                     /locus_tag="WJL20_001929"
     CDS             1343..1549
                     /locus_tag="WJL20_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ7684034.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(1648..1760)
                     /locus_tag="WJL20_001930"
                     /pseudo
     CDS             complement(1648..1760)
                     /locus_tag="WJL20_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          451 a          481 c          471 g          445 t
ORIGIN      
        1 ggtttaaatt taatccacta taaattaaac aacgggcgca cgctccgttg ccgaacggat
       61 aaaaccgagg aatcaccgat gagcaaaaaa ctccatccgc aaacgctcgc catacgcggc
      121 ggcaaagaac aaaccgaata ccgcgaacac aatcaggcat tgttcctgac cagcagcttc
      181 atgtgggaca acgcccaaca cgctgccgat ttgttttcaa aaaaaatcaa aggcttcact
      241 tatacccgta ccgccaaccc gaccacagcc gcctttgaaa aacgcatcgc cgctttggaa
      301 ggtgcggaac gcgcggtcgc cacttcgacc ggcatgtccg cgattcaggc ggcgtttttc
      361 acctttttgc aggcgggcga ccatgtgatt tccagccgca gcctgttcgg cacgaccgtc
      421 ggctttatca ataacatcgt taccaaattc ggcatcgaag tgagtcacgt gtcgccgact
      481 gatataaacg aatggaaagc cgccgtcaaa gccaacacca aactgctgtt tttggaaacg
      541 ccgtccaatc ccttgggaga agtggccgac ttggaagcct tggcggaatt ggcgcacggc
      601 atcggcgcgc tcttggtggt ggacaacagc ctgctgtcgc ctgtcggctc tcagcctttg
      661 aaacacggcg cggatatttc cgtttcctcc gcgaccaaag ccatagacgg acacgggcgc
      721 gtgatgggcg gcgtgttggc gggttcggaa gaactgatgg cgcaggtcgc cgtttactgc
      781 aactcttgcg ggctggcaat gtcgccgttt aacgcatggc agttgttgag cggcgtggaa
      841 accctgtctc tgcgtatgga aaaacagttc gacaacgcct tgaaaatcgc gcaatggctg
      901 caagcgcagc cgcaggttca agccgtgtat tacaccggat tacccgacca tcctcaggcg
      961 gagcttatcc gcaaacagca aaacggcggc ggcatcgtca tcggctttga agtcgccgac
     1021 caagcagccg cgtggaaagt cgtggacggc gtcgaacttt tctcccgtac cgccaacctc
     1081 ggcgacgtgc gctcgaccat tacccatccg tggacaacca cgcacggcag gatgcagccc
     1141 gaagaaaaac tcgccgccaa catccgcccc ggattggtgc gcctgtcggt cggtttggaa
     1201 tatgttggcg atttgattga cgatttgaaa caggcactcg cgcgctgatt gcgtgttcag
     1261 acggcatcgg cggaaaaaca ccgcaaatgc cgtctgaaac ccttatctta tatgacatac
     1321 acgtcaacaa taggaaaaaa atatggatga agcggttttt gccgattggg cgttgaaaat
     1381 ctgcctgacc ggcttgatta tttttttggg ttttatcgtt tggaatttgg gcaaggagtc
     1441 taaagcgggc aaattcggca tcgccatttt gtttttggtt ttgggcttgg gcgtgtttgg
     1501 ttttgtgttc aaagagttat tgattaagtt tttggtattg ccgaaatagg tttgcggtct
     1561 gtcaaaatgc cgtctgaaat cataaaaagt aatctgcacc ctaaaagttt gatttaaccg
     1621 ccaactgatt aaggtgcgga tttttatgac aaaatatagt ggattaacaa aaaccagtac
     1681 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcttga
     1741 tttaaattta atccactata aaaatcatct ttccgcagtc attcccacgg atgcgaaagc
     1801 ctgtcttttt gaatcccgga taattttaca tcgtcattcc cgcgcagg
//
LOCUS       DAWKQJ010000212         1813 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000212 DAWKQJ010000000
VERSION     DAWKQJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.212"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            9..221
                     /locus_tag="WJL20_001931"
     CDS             9..221
                     /locus_tag="WJL20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ7684035.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            256..1767
                     /gene="ubiB"
                     /locus_tag="WJL20_001932"
     CDS             256..1767
                     /gene="ubiB"
                     /locus_tag="WJL20_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ7684036.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     FVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT          400 a          494 c          492 g          427 t
ORIGIN      
        1 cactacatat ggagttagaa acggtcgtgc cgccggagga aggggagggc gaaggagaag
       61 gcgcggatgg ggaaatgccc gaatatcttg aacttcatgg cgggcgggaa gatgatattt
      121 ccgcaccttc gcaacccgaa ccgccgaccc gcaatatcaa accgctgctt ttcgggctgc
      181 tgattgcctt tttgggctat ctgctcggta agattttttg attgtccgat aaatgctgta
      241 ttcgggattt tatatatgaa atggttgaaa cgcctgacgg tcattgtcgg gactttttac
      301 cgctatcggc tggcaggtct gtgtgcttcg ctgatgggta gcggttggat atgcgctctg
      361 ctgaaaatga tgccgcagtc gtccaaattg aaaaacgaac cgcctgccgt ccgtctgcgc
      421 cttgccttgg aaagcctggg gccgattttc atcaagttcg ggcaggtttt gtccacacgc
      481 cccgatttga ttccgcacga ttacgccgtc gaactggcaa agctgcaaga caaagtgccg
      541 ccttttgacg cgcggctttc gcgtgaacaa atcgaaaaat cgttgggtca gtccatcgaa
      601 aagctgtatg cggaatttga aaccgagccc atcgccagcg cgtccatcgc ccaagtacac
      661 aaagcccgcc tgcattcggg cgaacgagtg gcggttaaag ttttgcgccc caaccttttg
      721 ccccttatcg aacaggattt gtcgctgatg cgtttcggcg cggcctgggt cgaacgcttg
      781 ttttcagacg gcaggcgtct gaagccgctc gaagtggtgg cggagttcga caaatacctg
      841 cacgacgagt tggatttgat gcgcgaagcc gccaatgccg gacagctcgg gcgcaatttc
      901 caaaacagca atatgctgat tgtgcccaag gtgttttacg actactgcac cagcgacgtg
      961 ctgacaatcg aatggatgga cggcacgccg gttgccgaca tcgccaaact caaagcagac
     1021 ggcatcgatt tgcacaaact cgccgattac ggcgtggaaa tctttttcac gcaggtcttc
     1081 cgcgacggct ttttccacgc ggatatgcac cccggcaata ttttggttgc cgccgacaac
     1141 cgctacatcg ccctcgattt cggcatcgtc ggcacgctga ccgattacga caaacgttat
     1201 ctcgccatca atttcctcgc ctttttcaac cgcgattacc ggcgcgtcgc caccgcccac
     1261 atcgaatcgg gctgggtgcc tgccgacacg cgtgcggaag aattggaagc cgccgtccgc
     1321 gccgtgtgcg aaccggtgtt caacaaaccg atttcgcaga tttccttcgg cttggtgctg
     1381 atgcgcctgt ttgaagtcag ccgccgcttc aatgtcgaaa tccaaccgca gtttgtgctg
     1441 ctgcaaaaaa cgctgctcaa catcgaaggc ttgggccgcc agctcgatcc cgatttggac
     1501 ttgtggaaaa ccgccaaacc gtttttggtg aaatggatga acgggcaggt cggccctaaa
     1561 gccctttggc gcaacctcaa aaacgaagcc cccgactggg cgcaaatcat cccttcattg
     1621 ccgcgcaaaa tcagtgcgtt gattgatgaa aaccgccagc aggaaatgcg tgatgcctat
     1681 atccatttgg tcaaagtgca gcagcggcaa agcctgtggc tggctgtgat tgcggttgtt
     1741 ttgctgctga ttttgctttt gaaataggct ttgtccgaat catcgcccga ctccgcccgt
     1801 ttataaggaa atc
//
LOCUS       DAWKQJ010000213         1792 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000213 DAWKQJ010000000
VERSION     DAWKQJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.213"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            225..1697
                     /gene="pyk"
                     /locus_tag="WJL20_001933"
     CDS             225..1697
                     /gene="pyk"
                     /locus_tag="WJL20_001933"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ7684037.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          462 a          519 c          487 g          324 t
ORIGIN      
        1 atctaggaca gctccaaaaa taaatttagc ctaagtcata aaaccgtgta attttgcacc
       61 aaaaaagacg gtttgcaggt aaaatatcaa actattaatt taaatcatat ttttacagaa
      121 tattccgccg cattcatcaa atggacatca aaccggttcc aaatttctgt aattttgcaa
      181 caaaataccg caaaacaccc gattgagacc gaaaggactt tcatatgaac cagacaagcc
      241 gcgatctgac ccgcatcagc cacaacacca aaatcgtcgc caccttgggg ccgggcagca
      301 acaacgtcga actgttggaa gacatgatcc gcgtcggcgg tctgaacgtc gtccgcttca
      361 acttcagcca cggcacgccc gaattccatc aggaaaacgc ccgcattgtg cgcgaggcgg
      421 caaaacgcgc cggacaggaa atcgccatca ttgccgacct gcaagggccg aaaatccgcg
      481 tgggcaaaat cgccggcggc ggcatcgaat tgaacaaagg cgaaacgctg gtactggatg
      541 ccgcgctcga aggcgaaggc acgcgcgagg cggtcggttt ggactaccgc gacctgcccg
      601 acgacgttac cgcaggcgac gtattgtggc tggacgacgg cctgctgacc ctgaccgtgg
      661 aatccgtcga aggcagcagg attatcacaa gggtggaaaa cagccatgtc ctgaaaagca
      721 acaaaggcat caacaaacgc ggcggcgggc tgtccgcagg cgcgttgacc gaaaaagact
      781 tccgcgacct gaaaaccgcg attgccatcg gttgcgacta cctcgccatc agctttgtga
      841 aatctgccga agatttgcac atcgcgcgcg ccaaagtcga agaagaaatg aagggcagca
      901 ctgccgtgcg ccccggtttg gtttccaaaa tcgaacgcgt agaagcgatt gaaaaccttg
      961 atgaaatcat cctcgccggc gacggcatta tggttgcgcg cggcgacttg gcggtcgaag
     1021 tcggacacgc cgccgtcccc gccctgcaaa aacggatgat ccgccgcgcc cgcgagttgc
     1081 gccgcttcag cattacggcg acgcaaatga tggaatcgat gattaccaac cccgtgccga
     1141 cccgcgcgga agtcagcgat gtggcaaacg cggtattgga cggtaccgat gcggtgatgt
     1201 gttccgccga aaccgccgtc ggcgcgtatc cgtttgaaac cgtcagccaa atggcaatta
     1261 tctgcgcggc tgcggaaaaa gagcaggatt cgctcaacgg cgttgccgaa caggcggagt
     1321 atcccgaagc ggtcagcacc aacctggcgg ttgccggcgg cgcggtcagc gtggcgcgcg
     1381 cggttcacgc caaagcgatt gtcgccctga ccgaaagcgg ttcgaccgcc tttgaaatca
     1441 gccgccacaa catcaccctg ccgattttcg cgctgacccc gagcgtttcc gcccaacgcc
     1501 gtatggcgat gtaccgcggc gtgcgcccgc tgattttggc aaccagtacc gaccacgaca
     1561 cggcgttgaa cgaagtcgaa accatgctgg tggaacacaa catcctgcat tccggcgacc
     1621 aatacatcat caccagcggc tcgcaaatgc gcgaatccgg ttcgaccaat acgctggaag
     1681 tgctgcgcgt caaataatcc gccctgagtg gaaaatgccg tctgaagtcg atgcccgagg
     1741 cttcagacgg cattgtttta ggcgcgattt atagtggatt aacaaaaatc ag
//
LOCUS       DAWKQJ010000214         1756 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000214 DAWKQJ010000000
VERSION     DAWKQJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.214"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(114..770)
                     /locus_tag="WJL20_001934"
     CDS             complement(114..770)
                     /locus_tag="WJL20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ7684038.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            835..1725
                     /locus_tag="WJL20_001935"
     CDS             835..1725
                     /locus_tag="WJL20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ7684039.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMAGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          396 a          419 c          581 g          360 t
ORIGIN      
        1 ggtttgggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt tgcgcggaga
       61 ggggatgccg tccgaagggc gaaaagggct tcagacggca ttgatgtcgg gtttcaggac
      121 aggagcagga tggcggctgc gacaagcgcg gcaaccgata atgcggcggc aagcgcggtt
      181 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
      241 aggacgaggt ttttcttttt gaacacgcgg tatgcggcga cggcggcgat atgaatggcg
      301 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgcataag gctgcccgta
      361 tgctcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
      421 aaaagccctg tgccgacttg aaatgagacg gcggccaaaa gcgcgacgac catcagtgcg
      481 cccaaggggt tgtgtccggg ttggacgtgt tcgggaatac cgtttttcat atactctctg
      541 atacccgacc atccgcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
      601 cagcagaggc ggaatacgag caggaaaagg ataaacagcc cgacgcgcgt gtgccattgc
      661 agcatatcgc cgccggtttt cgcgctatac cacataaagg gtagggatgc ggcaagcagc
      721 cagtggaaaa ggcgggtggg gaagtcccag actttggttt tgtttttcat aatcggtttc
      781 cggcggtaga atcggtttgt tttcgagcct taattttaac cgattggagg ggaaatgttt
      841 cccgttttcc atctttcagg cgaaagccgc cgccggatgc ttcaggcggc atcgcgtttt
      901 ccctatgttt tcaaagcccg tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc
      961 gtagtcggcg gatcggcagg gatgagcggc gcgcccgtat tggcggcatc ggcggcgatg
     1021 tatctcggct gcggcaaggt gtgggcgggt ttcaatcagg aaacgctgcc gtttgccgtc
     1081 attgccggtt ttcccgaaat tatgttggac acggcagacg gtttgaccaa gcgtcaaggc
     1141 ataaacgctt ggatggcagg ttgcggcctg ggtacggata aagcggcggt cggaacgctt
     1201 gccggaattt tggcggaaca cacggacaag cccgtccttt tggatgcgga cgcgctgaac
     1261 atattatcaa ccgatgccga aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta
     1321 acgccacacc ccgccgaagc cgcgcgcctg cttggaacga cggttgcaca ggttcaggcg
     1381 gatcggacgg cggcagtgag gaagataagg gcaattttcg gcgcaaccgt ggttttaaag
     1441 gggcacaaaa cattggttgc ctcacccgat acggaaatct atgtcaacga aagcggcaac
     1501 gcgggattgg caacggcggg cagtggcgac gtattgggcg gcatcatcgg cagtctgctc
     1561 gcacagggcg tgccggtttt tgaagccgcc tgcgcgggcg cgtggctgca cggcgcggcg
     1621 gcggatgtca taaaagaatc ggcaggcatt gcggcagggc tgttggctgg cgaaatcgcc
     1681 ccggcggcaa ggtggctgcg caaccggata actgaaagta tgtaagaaga tatagtggat
     1741 taacaaaaac cagtac
//
LOCUS       DAWKQJ010000215         1733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000215 DAWKQJ010000000
VERSION     DAWKQJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.215"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            356..1660
                     /locus_tag="WJL20_001936"
     CDS             356..1660
                     /locus_tag="WJL20_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ7684040.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          432 c          511 g          434 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggagcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taa
//
LOCUS       DAWKQJ010000216         1712 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000216 DAWKQJ010000000
VERSION     DAWKQJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.216"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(26..349)
                     /locus_tag="WJL20_001937"
     CDS             complement(26..349)
                     /locus_tag="WJL20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7684041.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WJL20_001938"
     CDS             complement(337..576)
                     /locus_tag="WJL20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ7684042.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJL20_001939"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJL20_001939"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ7684043.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          376 a          407 c          499 g          430 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatttc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaagccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatgg gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cccctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtccactg cccctatttg acccaataat gatttgggca gaatcacgcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatac gtaccatatt cacctgttta
      601 tgtaataaca atgttagtac cttgatgagg tagtgtcaac attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 ataaaggtcg gtggggcttt gaggcggctg cgttcttgaa tcagtgcggc gaagccttcg
      841 acgctgagtc tgccgctgcc gacatagacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgagctttg ggtaaggtcg
      961 gcgcggtaga tggttttgac gggggatttt gcttggcatt cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccgatcaa ataggcggca gcccgacgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg cacccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac ggtggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgggggg gtggatgcag ttgctacgaa
     1621 tcgctatcct gtgaattcac cctgtcagga gtgcccgaat cgtcattccc gcgcagtcgg
     1681 gaatctagga cgtaaaatct aaagaaacca tt
//
LOCUS       DAWKQJ010000217         1656 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000217 DAWKQJ010000000
VERSION     DAWKQJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.217"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            293..1462
                     /locus_tag="WJL20_001940"
     CDS             293..1462
                     /locus_tag="WJL20_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7684044.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQW
                     GNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNKQNQPPTLVPAVVGKPGSDVY
                     YAGLNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTG
                     GYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVSRISYAHGFDFIERG
                     KKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          421 a          391 c          422 g          422 t
ORIGIN      
        1 gccgcgtgtg tttttttatg gcgttttaaa aagccgagac tgcatccggg cagcagcgca
       61 tcggctcgca cgaggtctgc gcttgaattg tgttgtagaa acacaacgtt tttgaaaaaa
      121 taagctattg ttttatatca aaatataatc atttttaaaa taaaggttgc ggcatttatc
      181 agatatttgt tctgaaaaat ggttttttgc gggggggggg tataattgaa gacgtatcgg
      241 gtgtttgccc gatgttttta ggtttttatc aaatttacaa aaggaagccg atatgcgaaa
      301 aaaacttacc gcgctcgtat tgtccgcact gccgcttgcg gccgttgccg atgttagcct
      361 gtacggcgaa atcaaagccg gcgtggaagg caggaacatc cagctgcagt tgaccgaacc
      421 gctccaaaat attcaacaac ctcaggttac taagcgcaaa agccgcatca ggacgaaaat
      481 cagcgatttc ggctcgttta tcggctttaa ggggagcgag gatttgggcg aagggctgaa
      541 ggctgtttgg cagcttgagc aagacgtatc cgttgccggc ggcggcgcgt cccagtgggg
      601 caacagggaa tcctttatcg gcttggcagg cgaattcggc acgctgcgcg ccggtcgcgt
      661 tgcaaatcag tttgacgatg ccagccaagc cattgatcct tgggacagca ataatgatgt
      721 ggcttcgcaa ttgggtattt tcaaacgcca cgacgatatg tcggtttccg tacgctacga
      781 ttcccccgaa ttttccggtt ttagcggcag cgtccaattc gttccggccc aaaacagcaa
      841 gtccgcctat acgccggctc attttgttca gaataagcaa aataagcaaa atcagccgcc
      901 tactctcgtt ccggctgttg tcggcaagcc ggggtcggat gtgtattatg ccggtctgaa
      961 ttacaaaaat ggcggttttg ccgggaacta tgcctttaaa tacgcgaaac acgccaatgt
     1021 gggccgtgat gcttttgagt tgttcttgct cggcagcggg agtgatgaag ccaaaggtac
     1081 cgatcccttg aaaaaccatc aggtacaccg cctgacgggc ggctatgagg aaggcggctt
     1141 gaatctcgcc ttggcggctc agttggattt gtctgaaaat gccgacaaaa ccaaaaacag
     1201 tacgaccgaa attgccgcga ctgcttccta ccgcttcggt aatgcagttt cacgcatcag
     1261 ctatgcccat ggtttcgact ttatcgaacg cggtaaaaaa ggcgaaaata ccagctacga
     1321 tcaaatcatc gccggcgttg attatgattt ttccaaacgc acttccgcca tcgtgtctgg
     1381 cgcttggctg aaacgcaata ccggcatcgg caactacact caaattaatg ccgcctccgt
     1441 cggtttgcgc cacaaattct aaatatcggg gcggtgaagc ggatagcttt gtttttgacg
     1501 gctcgccttc attctttgat tgcaatctga ctgccaatct gcttcagccc caaacaaaaa
     1561 tccggatacg gaagaaaaac ggcaataaag acagcaaata ccgtctgaaa gattttcaga
     1621 cggtatttcg cattttcggc ttggtttgca catata
//
LOCUS       DAWKQJ010000218         1608 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000218 DAWKQJ010000000
VERSION     DAWKQJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.218"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(154..1239)
                     /locus_tag="WJL20_001941"
     CDS             complement(154..1239)
                     /locus_tag="WJL20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7684045.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVEVSRVKDAGTYKA
                     QGGKSKTATQIADFGSKIGFKGQEDLGNGMKAIWQLEQKASIAGTNSGWGNRQSFIGL
                     KGDFGTVRAGNLNTVLKDSGDNVNAWESGSNTEDVLGLGTIGRVESREISVRYDSPVF
                     AGFSGSVQYVPRDNANDVDKYKHTKSSRESYHAGLKYENAGFFGQYAGSFAKYADLNT
                     DAERVAVGATDANPVKDYQVHRVVAGYDANDLYVSVAGQYEAAKNNTVSVGEQGKKHE
                     QTQVAATAAYRFGNVTPRVSYAHGFKAKVNGKKAARYQYDQVIVGADYDFSKRTSALV
                     SVGWLKEGKGVNKTEKTASMVGLSHKF"
BASE COUNT          429 a          367 c          372 g          440 t
ORIGIN      
        1 aaaagccaaa cagaaaaaag ccaaacagaa aacagccaaa cagaaaacag ccaaacagaa
       61 aacagccaaa cagaaaacag ccaaacagaa aacagccaaa cagaaaaaag cctgtctggc
      121 gacaggcttt ttgttgatac caatcttttc agattagaat ttgtgactca agccaaccat
      181 gctggcagtt ttttcagttt tgtttacgcc tttgccttct ttcaaccaac cgacagaaac
      241 cagagcggaa gtgcgtttgg agaagtcgta gtcggcacca acgataactt ggtcgtattg
      301 ataacgtgct gctttcttgc cattcacttt agctttgaag ccgtgagcgt aagaaacgcg
      361 aggcgttacg ttgccgaaac ggtaagcggc agtagcggca acttgagttt gctcgtgttt
      421 tttaccttgt tcgccgacac taacagtgtt atttttagca gcttcatact gaccggcaac
      481 agaaacgtac aggtcattgg catcgtaacc ggcaactacg cggtgtactt ggtaatcctt
      541 aacaggattg gcatctgtag cacctactgc aacacgttct gcatcagtgt tcaaatcagc
      601 atatttggca aaagagcctg cgtattgacc gaagaaaccg gcattttcgt atttcagacc
      661 ggcgtggtaa gactcacggc tggacttcgt atgtttgtat ttatccacat cattcgcatt
      721 atcgcgcgga acgtattgta cgctgccgct gaagcctgca aatacgggag agtcgtagcg
      781 tacggagatt tcacggcttt ctacacgacc gatagtaccc agtcccagta catcttcggt
      841 gttagaacca gattcccatg cattgacgtt gtcgccgctg tctttcaata cggtgttcag
      901 attaccggcg cggacggtac cgaagtcgcc tttcaagccg atgaaggatt ggcggttacc
      961 ccagccgctg ttagtgccgg cgatggaggc tttttgttcc aactgccaaa tggctttcat
     1021 gccgttgccg aggtcttctt gacctttgaa accgatttta gaaccgaagt cggcaatttg
     1081 ggttgcagtt ttagattttc cgccttgagc tttatatgta ccagcatctt ttacgcgaga
     1141 aacttctacg cctgctttaa ttgtaccgta caaggtaacg tcagccattg ctgcaacagg
     1201 cagggcagcc agtgtcaggg caatcaggga ttttttcatt gctgtattcc ttttcatagt
     1261 taagaaattt aagcagacca aactttccaa tccgcttagg ctttacttct accgccgatg
     1321 tcggcgattc catgtctgaa actataaatg ttcctgtctg aaaaaacaaa cttctgcctg
     1381 tttttgtgtg gaatccgatg tgcattttga aggacggata gagatattat gggtattttt
     1441 tgttttataa catatggtta tttaaaattt ttaagatttg catttttaca acacttactc
     1501 gggagggtat tggagggcat tgcaaaccgg gggttataaa gacggcaaaa aacgcgcacg
     1561 cggtttcttt cggaacgggc ggatgcaaaa cccgcccctg cggcagga
//
LOCUS       DAWKQJ010000219         1597 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000219 DAWKQJ010000000
VERSION     DAWKQJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.219"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(40..521)
                     /locus_tag="WJL20_001942"
                     /pseudo
     CDS             complement(40..521)
                     /locus_tag="WJL20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(858..1499)
                     /locus_tag="WJL20_001943"
     CDS             complement(858..1499)
                     /locus_tag="WJL20_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ7684046.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT          511 a          361 c          278 g          447 t
ORIGIN      
        1 ttaaaattta cctttggaag taccgccacg cataatagtt tagatatgtt tataatctct
       61 ggataaaaaa acgtaataag tgcttactgg ataacaaagt ccaaaccaat agcaggcaaa
      121 ataaggcatc cacccccctt cttcattaag gatatatatt gagaaacaaa tcgcaactaa
      181 acagaaaaaa cttgggagat aaagccattt cattccccta ttcaagaatc tagccaagat
      241 aggtattttg tattctacaa aaaagaaagg catttccaag ggaaacatgt cagataaaaa
      301 cttttgttta ttttttacta tagatagaac cttgcttctc aagagaaagc cattaatata
      361 tagagaatag tataagtatg aataatcttc attagacaaa aagaagaaat ggcagataaa
      421 ttacatacga tatattggaa tataaaatat ttacggtcta aaccttgttc agttgcaatt
      481 tttttatact taccttgcgc aaaaaaatca aagaaatcca ttaaactatc tttcacatta
      541 gaaatttaaa agctaaataa tacgacaaac aatgtgaagt actattcatg gtttatttaa
      601 aaaataatac tattctgaac attatttaga tacagaaatt aacaaattag aactaaacaa
      661 gcttttaaat actttaattt tattggaaag ctataaaagg aactataact ttacacacta
      721 gtcacttctt tttaagaggc aaaagggatt gggaaggtcg tcttggagat aagcactggt
      781 atttcggcca atggtaaata gagtttacct caaatagggt agaacctcct tcatctgtca
      841 gttaataaca gccactttta caatgccctg tcgaaataaa gtggcacgcc cgatttttca
      901 ctcataccga ttaaataatc catcgtcttc cgagggaacg cgatgccgcg caccacggtc
      961 agattcctca aaacggggaa aaccaaaata tcctccatac cgattccgcc gttgatgccg
     1021 tctgaagcac cgtccatcaa attttccaac tcttgcaaat ctgcgtttat ccgttcgagg
     1081 tattgggcgg ttttattcaa attggcggaa aagctgccga tgcttttctc ttttttgtct
     1141 gtaaaatatt tcaccgcttc cggcgttgca aattcaggca gcccgatttt gatcacgcgc
     1201 ggctgcacca gtttgtcgtt gtatccgccc accttgtcca gccacgcccg tatctcgggg
     1261 cggacttcgt ctttcagacg gtcttcgcgg tcgaaatgcc gcacaatgtc caaactctcg
     1321 cccataaacg aaccgtcttc tttttgcagg acgggcactt gtttcgcacc gatcataccg
     1381 atcggcgttg cctcgtcgtc gtttgccagc acggcttctt caacgtccgc gccaaacagc
     1441 ccggcagcca tccgcgcacg cacgcaaaac gggcaatggt cgtaaatata cagtttcatc
     1501 aaaatattcc tcgtcaacct gtcggtaccg gctaccttaa caccccgcgc cacccgaaac
     1561 aagtttatct tcccgcctat gcaccgtaaa taaataa
//
LOCUS       DAWKQJ010000220         1591 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000220 DAWKQJ010000000
VERSION     DAWKQJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.220"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            59..1432
                     /locus_tag="WJL20_001944"
     CDS             59..1432
                     /locus_tag="WJL20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7684047.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          414 a          522 c          384 g          271 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtc aaacaaaaaa acatcagttt gaacaaagta gaaattgtcg ttttggatga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccttc caaaagcgca acctgctcga
      781 acggctgatt gtcgatctgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatt gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaac
     1321 cacggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacctgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 tgggcgcaac cgcccgcacc ggataaattt ctgccgcaaa cagtttcaga cggcatttgc
     1561 tgcctgtaca atatagtgga ttaacaaaaa t
//
LOCUS       DAWKQJ010000221         1588 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000221 DAWKQJ010000000
VERSION     DAWKQJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.221"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..139
                     /locus_tag="WJL20_001945"
     CDS             41..139
                     /locus_tag="WJL20_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ7684048.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(210..1586)
                     /locus_tag="WJL20_001946"
                     /pseudo
     CDS             complement(210..1586)
                     /locus_tag="WJL20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT          293 a          430 c          512 g          353 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttccaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgggggt tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatcagc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcgggcaagc
     1081 agttcggcgc aactggtata gggataggga acggcttttt ccgtttccgc ctgccggtca
     1141 aaatcttctt cggtaacgac aaagccgccg ccgaccgaat aataaacctg ttcattcaat
     1201 accgtgccgt ctgaagcata ggcggtaaaa cgcaggccgt tggggtgttt gggcagcact
     1261 tgattgccga gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaaaatata gtggatta
//
LOCUS       DAWKQJ010000222         1542 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000222 DAWKQJ010000000
VERSION     DAWKQJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.222"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..404
                     /locus_tag="WJL20_001947"
     CDS             96..404
                     /locus_tag="WJL20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684049.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WJL20_001948"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WJL20_001948"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ7684050.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAILHKFSGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
BASE COUNT          383 a          524 c          355 g          280 t
ORIGIN      
        1 agttttgagg acgataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccattccgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaagcc gtcatgcagc aaatccgctt cgaccccaac gcctaccaca
      301 tcgacagcga cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacgcgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcgga cggcataccc gataaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg
      601 actacatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagaattgg gcgtaaattt gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacc ctcgtcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcgtaatcga tgccgccgtc gccgatgccg
     1261 ctttaagcat gctggatgtg gacgcgcaag gtttggatgt tatggacaga aaattcctcg
     1321 aagccatcct gcataaattc agcggcggcc ccgtcggttt ggacaacgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctatctcatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc ta
//
LOCUS       DAWKQJ010000223         1520 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000223 DAWKQJ010000000
VERSION     DAWKQJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.223"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      79..123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            128..601
                     /gene="queF"
                     /locus_tag="WJL20_001949"
     CDS             128..601
                     /gene="queF"
                     /locus_tag="WJL20_001949"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ7684051.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTSRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            676..1362
                     /locus_tag="WJL20_001950"
     CDS             676..1362
                     /locus_tag="WJL20_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ7684052.1"
                     /translation="MYAFTAAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARQIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT          359 a          466 c          312 g          383 t
ORIGIN      
        1 atgacacgga aaacgccgcc gtccaaacca tgccgtctga agaaaactac acagataccg
       61 ccgcttatat tacaatcgcc gcccgtggtt cgaaaacctc ccacattaaa aaactaagga
      121 aaccctcatg tcccgcaaca acgaagagct gcaaggcatc tctcttttgg gcaatcaaaa
      181 aacccaatat ccgaccggct acgcgcctga aattctcgaa gcattcgaca acaaacatcc
      241 cgacaacgac tatttcgtca aattcgtctg tcccgagttc accagcctct gccccatgac
      301 cgggcagccc gacttcgcca ccatctacat ccgctacatt ccgcacatca aaatggtgga
      361 aagcaaatcc ctgaaactct acctcttcag cttccgcaac cacggcgatt ttcatgaaga
      421 ctgcgtcaac atcatcatga aagacctcat tgccctgatg gatccgaaat acatcgaagt
      481 attcggcgag ttcacatcgc gcggcggtat cgccattcat ccgtttgcca attacggcaa
      541 agcaggcacc gagtttgaag cattggcgcg taaacgcctg tttgagcacg acgcacaata
      601 aaccatcctt cccatatttc agacggcatt ccttcgatac ggtatgccgt ctgaacgcct
      661 ttccgaaaga tttttatgta cgcatttacc gccgcacagc aacagaaggc actcttccgg
      721 ctggtgcttt tccatatcct tatcatcgcc gccagcaact atctggtgca gttccccttc
      781 caaattttcg gcatccacac cacttggggc gcattttcct ttcccttcat cttcctcgcc
      841 accgacctga ccgtccgcat tttcggttcg cacttggcac ggcagattat cttttgggtc
      901 atgttccccg cccttttgct ttcctacatc ttttccgttt tgttccacaa cggcagttgg
      961 acaggcttgg gcgcactgtc cgaattcaat acctttgtcg gacgcatcgc cttagccagt
     1021 tttgccgcct acgcgctcgg acaaatcctt gatatttttg tattcaacaa attacgccgt
     1081 ctgaaagcgt ggtggattgc cccaaccgca tcaaccgtca tcggcaacgc cttggatacg
     1141 ttggtatttt tcgccgttgc cttctatgca agcagcgatg aatttatggc ggcaaactgg
     1201 cagggcatcg cttttgtcga ttacctgttc aaactcaccg tctgcggtct gtttttcctg
     1261 cccgcctacg gcgtgattct gaatctgctg acgaaaaaac tgacgaccct gcaaaccaaa
     1321 caggcgcaag accgccccgc gccctcgctg caaaatccgt aaaacagccc tgccgggaat
     1381 cgggacggtt gggttttctt ttatttgcaa acactgtcgg aacggataaa tccggattcc
     1441 tacctacttc gtcattcccg cgaaagtggg aatccggtgc gttgggtttc catcatttcc
     1501 gataaactcc tgccgcgttg
//
LOCUS       DAWKQJ010000224         1513 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000224 DAWKQJ010000000
VERSION     DAWKQJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.224"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(138..515)
                     /locus_tag="WJL20_001951"
     CDS             complement(138..515)
                     /locus_tag="WJL20_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7684053.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(546..944)
                     /locus_tag="WJL20_001952"
     CDS             complement(546..944)
                     /locus_tag="WJL20_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7684054.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            complement(950..1513)
                     /locus_tag="WJL20_001953"
                     /pseudo
     CDS             complement(950..1513)
                     /locus_tag="WJL20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
BASE COUNT          421 a          325 c          249 g          518 t
ORIGIN      
        1 tgaccaaggt gctgccgtgg aaggagcagg tgtaggattt ttgaagaact tagttcccaa
       61 tatcctgttt caaataaggg gaaggtcgcc tgaaaattaa acacagtttc agacggcatt
      121 ttgattgccg gggtttgcta ttttttgttg taataatcaa atcgcacgtt gactatgtct
      181 ttctcggtaa aaatataacg gagcattgtt gtgaatcttt cataacgttc atgaattccc
      241 acgctatcag gtagccaagg ggaagcttta atttcgaaaa gctcccaatt tggaaccatt
      301 aaaaaatcaa taatggtact gattccaatc acaatatccc ttggtatatc catcggataa
      361 ggatattttt ttctaacctc gattaaatca ttctccaatt tccaatattc ttcatcatcc
      421 cacaccccgt catcatacca tttgccaata aatgaatttt cgtcataccc ctcaaaataa
      481 ggaacgtttc ttataatatc cttgaactca cacataataa tgtatctcca atataattaa
      541 acttttcatc tcaatctacc tttactatgt tgtattggaa agtaaaaaaa tttccagtcc
      601 tctacatcta gatcagtaaa aatataacgg agcattactc tgaacctttc ataacgctca
      661 ttaattttga cacttttagg caaccaagga gaagctttaa tttcaaaaag tttccaattt
      721 tgaaccatta aaaaatcaat aatggtaccg attccaatca cgatgtccct tggtatatcc
      781 atcggataag gatatttttt tctaacctcg attaaagcat tctccaattt ccaatattct
      841 tcatcatccc aaaccccgtc atcataccat ttgccaataa atgaattttc gtcatactcc
      901 ttaaaacaag ggatgtttct tctaaaatcc ttgaactcgc acatcataat taatctccaa
      961 tacgatttag gtttttatca aatgtaccgt ttcttgtttc ttttctgttc agcttcttgg
     1021 gtgaagatgc ttctttccaa gcccctccat tatgtgaatc tacatcgcgt gatatataac
     1081 tctttccttt tttaaaaata gcagcatcat ttctcgttct ttcttttatt tttctatatc
     1141 ccaatttctt tgctgctgca tatgcttctg aatcattccc atatacgggg gtagatggtg
     1201 tttttcttgg cggacacgca ttatgaaccc aaaccccttc cgttccgcct gattaccctt
     1261 gacgaagtag gtatgccagt cggcaacggt cagattgtag gctttgagcg gctgctgttt
     1321 gagggtaatg ttttgaaccg tctgttttgc accgctttcg gaaagcaggg tgtcgccttt
     1381 tttcagacga cctgcctgta tccattttcc ttgactgtaa aacgggtgga ttttattgga
     1441 aatcagggtt tggttgttgc cgatgccgtc tgaaatttca atgtaaacgg tttcccgata
     1501 cggattgccg tat
//
LOCUS       DAWKQJ010000225         1488 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000225 DAWKQJ010000000
VERSION     DAWKQJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.225"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..405)
                     /locus_tag="WJL20_001954"
     CDS             complement(<1..405)
                     /locus_tag="WJL20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684055.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQ"
     gene            complement(421..>1488)
                     /locus_tag="WJL20_001955"
     CDS             complement(421..>1488)
                     /locus_tag="WJL20_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7684056.1"
                     /translation="QILAYSKLVAGTVSGVVGGDVNAAANAAEVAVKNNQLSDKEGRE
                     FDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTDISRSTECRTIRKQHLI
                     DSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNTAAKSWLQSGNTKPLSE
                     WMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISFDTNLKRHLANADGFSQ
                     KQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEIPTLDRTGKPDGGFKEI
                     SSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTKSISERKNVIQFSETFD
                     GIKFRSYFDVNTGRITNIHPE"
BASE COUNT          425 a          261 c          238 g          564 t
ORIGIN      
        1 ttgagaatat gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa
       61 gaaatcagat attgcaatat taaagtaatg atatgctaaa ttaataggta taattaattc
      121 ttcaggattg aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc
      181 aaatccattt acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg
      241 agcacctatt actccaaaat gtccggtagt tgtaatattt ttaaaacact ctaaaagcgt
      301 atctttagtt tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt
      361 atttatagat tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa
      421 ttattctggg tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt
      481 gattccgtca aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt
      541 tctttcattt tgagcaattt tagaggcttt tgaatatcct tgtgaagcag cattttgagc
      601 catttgaagt attttatcat cagaaaattt tttaggatta taaacagttt ttatacttga
      661 aatttcctta aatccaccat caggtttacc tgtcctgtct agtgtaggaa tctcatattt
      721 aattcgggta atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga
      781 atttagttct gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact
      841 aaaaccatca gcatttgcca gatgtctttt taggtttgta tcgaaactga tgccttccgg
      901 gtatttgaca ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa
      961 tctaggatta actcctgaaa taagtgtata accttggtcg gacatccatt cggataaagg
     1021 ctttgtattg cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa
     1081 agccaaatct gcttgggtgt aagatttgct gaataattta taccattcat catctttacc
     1141 aattagacct gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct
     1201 gattgttcta cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag
     1261 tcttttatca gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt
     1321 attctgtttg gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag
     1381 ctgattattt ttcaccgcta cctcagccgc attcgccgcc gcatttacat cgccgccgac
     1441 cacaccgctt accgtaccgg caaccagttt gctgtatgcc aaaatctg
//
LOCUS       DAWKQJ010000226         1474 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000226 DAWKQJ010000000
VERSION     DAWKQJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.226"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..594
                     /locus_tag="WJL20_001956"
     CDS             <1..594
                     /locus_tag="WJL20_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7684057.1"
                     /translation="GKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPV
                     GALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVS
                     AGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVG
                     KVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            601..1110
                     /locus_tag="WJL20_001957"
     CDS             601..1110
                     /locus_tag="WJL20_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684058.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1179..>1474
                     /locus_tag="WJL20_001958"
     CDS             1179..>1474
                     /locus_tag="WJL20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ7684059.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNV"
BASE COUNT          478 a          293 c          290 g          413 t
ORIGIN      
        1 ggtaaattag ctatcagcac ctgcatggct aatccttctg gttgtactgt catggtcact
       61 caggctgccg aagcgggcgc gggaatcgcc acgggtgcgg taacggtagg caacgcttgg
      121 gaagcgcctg tgggggcgtt gtcgaaagcg aaggcggcta agcaggctat accaacccag
      181 acagttaaag aacttgatgg cttactacaa gaatcaaaaa atataggtgc tgtaaataca
      241 cgaattaata tagcgaatag tactactcga tatacaccaa tgagacaaac gggacaaccg
      301 gtatctgctg gctttgagca tgttcttgag gggcacttcc ataggcctat tgcgaataac
      361 cgttcagttt ttaccatctc cccaaatgaa ttgaaggtta tacttcaaag taataaagta
      421 gtttcttctc ccgtatcgat gactcctgat ggccaatata tgcggactgt cgatgtagga
      481 aaagttattg gtactacttc tattaaagaa ggtggacaac ccacaactac aattaaagta
      541 tttacagata agtcaggaaa tttgattact acatacccag taaaaggaaa ctaactaaat
      601 atgagtaact ttgaaaaaaa atatatttta gaattaaatg atgctttaag ccatttaaat
      661 cataactcta cctcatttga tttattgaaa gttttgattt catggttatc aaacgatatt
      721 gtcattgata aatttaaaat tttaggttat gactttagta aatatatcga aatgaatccc
      781 gatgactatc cggttgaaaa atctatattg aatagagagg aaattattta tctcaaaaac
      841 aatatttatc gtaaaatatc atcaggaaat tttaaatttc aatactttgt acaatatatt
      901 agagatattt tagaatattt atttattgaa catattgaaa gagtctgtcc ttactgcgaa
      961 tggggtgaaa tgcaaaaatt agaagaacaa aatacgcatg aaacggtgta tctctgtact
     1021 caatgtggat gtgcttttta taacgataat tcacaatttt tattaaaaac ccctttaacc
     1081 attccaatga aacgtgatga atttaaataa acaagccgta gcctgcatga accctaaaat
     1141 ccacgtgtag cgtgtgtgcg ccagcacgca tgcgttccat gatttacggc tcaatgccgt
     1201 ctgaaaagct cacaattttt cagacggcat ttgttatgca agtaaatatt cagattccct
     1261 gtatgctgta cagacgcggg agtgttaagc cccccttgtt tgaagctccg cggctcctgc
     1321 cgagcttcac cgaccccgtt gtgcccaagc tctctgctcc cggcggctac attgtcgaca
     1381 tccccaaagg caatctgaaa accgaaatcg aaaagctggc caaacagccc gaatacgcct
     1441 acctgaaaca gcttcagacg gccaagaacg tcga
//
LOCUS       DAWKQJ010000227         1451 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000227 DAWKQJ010000000
VERSION     DAWKQJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.227"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(73..603)
                     /locus_tag="WJL20_001959"
     CDS             complement(73..603)
                     /locus_tag="WJL20_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ7684060.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(644..>1451)
                     /locus_tag="WJL20_001960"
     CDS             complement(644..>1451)
                     /locus_tag="WJL20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7684061.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILKQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
BASE COUNT          392 a          350 c          268 g          441 t
ORIGIN      
        1 gcatcggata ccgttatcgg atgcgtcccg tccgcgtgaa tttggggctg acgtaggaac
       61 gccgatgtga tttcacatcc cgtactgttt cgacagcttc acataatgcg cggcggaata
      121 tttcaaaaag gctttttcct catcggtcag cacgcgcacc tgtctgaccg gcgaaccgac
      181 ataaagatag ccaccctcca aacgtttacg cggaggcacg aggctgcccg cgccgatcat
      241 cacttcgtcc tcaatcacag catcgtccaa aacggtgctg cccataccga ccaagacgcg
      301 gttgccgatt cggcagccgt gcagcatcac tttgtgcccc acggtaacgt cttcgccgat
      361 aaccagcggc gatccttcgg gtttggcggc ggttttgtgg gaaacgtgca ggacgctgcc
      421 gtcctgtata ttgctgcgcg cgccgacggt gatgctgttc acatcgccgc gcaacacggc
      481 gcacggccac acggaaacgt cttcggcaag cgacacttcg ccgatgacga cgcacgcttc
      541 gtctatcata cagctttcgt ggatttcggg cgtgcggttt tggaaagttc ggattgcgtt
      601 cattttttct ccttcggtaa gatgtatagt gttaaaggat ttattaaata ttccctctga
      661 ttgcttttaa aatcctgcct gtaatatcaa ccccgaataa tgtgattatc gggaatatca
      721 gcttatatat caatttattg gactttaaca gcataaacct taaatgatac gcccttcttt
      781 ttatatcagc atcacactct atatttttac tcgtcatgat aaaaagcaaa acgagatatt
      841 cgtaggaaag aaaagaataa agataacttg atatatccct atcaaattcc atttccgcat
      901 ttttctccaa aatatataat aatgacttta tacttttttc cgaaacagtt cctgttttag
      961 aatcttttct tccctgccga taatagtaat aacaaccgtc caaataagaa aaagttgttg
     1021 ccgcattaaa taatctcatt gaccattcga tatcttcggc ataaacctct ttttcaaaaa
     1081 acagtttttc tttgataatc aattcccttt ttataatctt attacacgcc gaacccggaa
     1141 attttctaaa tcggcataat cctttcaaaa cttcgacttt ggattgcttg agtatttttt
     1201 caggctgata atcttcgcca aaatgtgaaa cacttccctt atcatattta accgcattta
     1261 aaaaaaccac atccggcata tcaatatcat ctttactaag aaaatccagc aagacctgac
     1321 aattaataaa atcatccgaa tcaataaaga ctatatattt tccgtttgaa ttttttattc
     1381 cggtattcct agcttctgcc gtcccttggt tatcctgata tatatatttg atgtttggtg
     1441 ttcggtatct g
//
LOCUS       DAWKQJ010000228         1441 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000228 DAWKQJ010000000
VERSION     DAWKQJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.228"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            25..1362
                     /locus_tag="WJL20_001961"
     CDS             25..1362
                     /locus_tag="WJL20_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7684062.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKNAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          261 a          350 c          384 g          446 t
ORIGIN      
        1 ccttcacatt tcttaataaa ttttatgagt aaccatactt cttggtcgtc caaaatcggt
       61 ttcgtgcttg ctgcggcagg ttcggccatc ggtttgggcg cgatttggaa atttccttat
      121 acggcgggca ccaacggcgg cgcggtgttt ttcctgctgt ttttgatatt tactatcttg
      181 gtcgccctgc ccgtccagct tgccgagttt tatatcgggc gcacaggcgg taaaaatgcc
      241 gtcgattcct tcagggctct gcgtccgagt acgcaatggc tttgggtcgg gcgcatgggc
      301 gtttccgcct gctttatttt gctgtcgttt tacagcgtgg tcggcggatg ggtattaaat
      361 tatgtcgtcc acagtttcac gggggcggtt cataccggcg cggactttga agccttgttc
      421 ggcgcgacga tttccaatcc ggcaggttcg ctgtcctatc aggcactgtt tatgctgatt
      481 acggtttggg tggtcaaagg cggcatttca gacggcattg aaaaggcaaa ccgttatctg
      541 atgccggggc tgtttatcct ctttattgca ctggctgtcc gttcgctgac gctgccgggt
      601 gcaatggagg gcgtgtcttt cctgctcaaa ccgaattggt cgtactttaa agccgatacg
      661 atgattacgg ctttgggaca ggcgtttttt gccttgagca tcggcgtttc cgctatgatt
      721 acctatgctt cgtatttggg aaaagatcag gatatgttcc gttccggcca tacgattatg
      781 tggatgaacc tcttggtttc gctgcttgcc ggcctagtga tttttccggc ggtgttcgcc
      841 ttcggttttg aaccgagcca ggggccggga ttgattttta tcgtattgcc cgcagtgttc
      901 atgaaaatgc cgttcggtac ggttttgttt gcggtattta tgctgctggt cgttttcgcc
      961 acgctgactt cggcattttc gatgttggaa acggtcatcg ccgcaaccat ccgccaagac
     1021 gagcgcaaac gcaaaaaaca cacttggctt atcggcacgg ccattttcat tgtcggcatc
     1081 ccatccgcgc tgtctttcgg cgtatggggt gagtttaagg ttttcggcaa aaccattttt
     1141 gatttgtggg actatgttat ttccgccgtc attatgcctg tcggcgcgtt gggcgttgcc
     1201 gtatttaccg cctggattca ggacaagcag tctgtgttaa aaaatgccgg cgcgggcagc
     1261 accgtaccac gggcagtgct gctgctgtgg ctgaatacct tgcgctacct tgccccgatt
     1321 gccattatta ttgttttcat caattctttg gacatccttt aaaagccatc caaacagcaa
     1381 aaatgccgtc tgaaagcctt tcagacggca tttttgcttc aggttcagcc tatttcgttc
     1441 a
//
LOCUS       DAWKQJ010000229         1394 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000229 DAWKQJ010000000
VERSION     DAWKQJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.229"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..456)
                     /locus_tag="WJL20_001962"
     CDS             complement(10..456)
                     /locus_tag="WJL20_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684063.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNI"
     gene            complement(1020..1223)
                     /locus_tag="WJL20_001963"
     CDS             complement(1020..1223)
                     /locus_tag="WJL20_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684064.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          484 a          256 c          166 g          488 t
ORIGIN      
        1 gtctagagtc tatatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaattcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa gtagaaaatc
      901 aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta taaaattaaa
      961 taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtggg aatgcattat
     1021 tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc aatgcttgca
     1081 tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg ttcttttctt
     1141 gcaagatgtg cacctgcaca accacctaat actaaaccac cacctattgt aaatccttcc
     1201 tgttgtatct ctaatgataa cattttcttc ttgtgccgcc cttaatacag attgtggaat
     1261 tggattgacc tgagcaggaa cttctaaaat aagttttcct tcaagctgtc ttcctcttag
     1321 cgaagcaggt gtacttggaa cattggaaat gacaaagtga gtgcgcaaag ccacctgaag
     1381 gcgatgtgtt tgaa
//
LOCUS       DAWKQJ010000230         1339 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000230 DAWKQJ010000000
VERSION     DAWKQJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.230"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            328..>1339
                     /locus_tag="WJL20_001964"
     CDS             328..>1339
                     /locus_tag="WJL20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7684065.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAQAQTHNYAIVMNEQ
                     NQPEVKQEGSYSTLREKDRGRKFIYNKGRSGGGSVSFNNSDELVSRQSGTAVFGTATY
                     LPPYGKVSGFDADGLEKRNNAAGWIRTTRIALAGYSYASVVCKSSTGCPKLVYKTRFS
                     FDNPDLEKIGGRLDRHTDPSRDNSPIYKLKDHPWLGVSFNLGSENTVKNGKSSSKLIS
                     SFNENNNNQTIVSTTEGDPISLGDQQREHTAVVYYLNAKLHLLDKKGIKDITDKTVQL
                     GVLRPSIDVRLQRNTGLAGLLNFWASWDIKDNGQIPVKLGLPEVKAGRCINANNPNKS
                     TKA"
BASE COUNT          368 a          340 c          353 g          278 t
ORIGIN      
        1 tgtcgggcat ctgcagcggt ttgctaaaaa acgctttacc gtgatcagtg tgcaaagtta
       61 aaatggggag gtaagctttt caatcagcaa ttcggcgggc gcggaatcgg gcggtttgcc
      121 gaatcccgcc gtttgcggcg cgcctgccgc cgacggtatc ccgcgaagca agatttaagg
      181 gataaaatat gttccaacac gcagggcggc acataaggcg ccgccctgat tcggaagggg
      241 ctgcacccct cccggacaaa gcctgatcct gccgccccga aggacggatg cccgaagggc
      301 ggggggtttg accgaaaagg aaatacgatg aataaaactt taaaaaggcg ggttttccgc
      361 cataccgcgc tttatgccgc catcttgatg ttttcccata ccggcggggg gggggcgcag
      421 gcgcaaaccc ataactacgc tattgtcatg aacgaacaaa atcagccgga ggtaaagcag
      481 gagggttcat attcaacctt aagggaaaaa gacaggggac gcaaatttat ctataataaa
      541 ggccgatcgg gtggaggctc tgtctcattc aacaatagcg atgagcttgt ttctcgacaa
      601 agcggtactg ccgtttttgg cacagccacc tacctgccgc cctacggcaa ggtttccggt
      661 tttgatgccg acgggctgga aaagcgcaac aatgccgccg gttggattcg taccacccgc
      721 atcgcgctgg caggctacag ctacgccagt gtcgtatgca aaagcagcac aggctgtccc
      781 aaacttgtct ataaaacccg attttccttc gataatcccg acttggaaaa aataggaggc
      841 aggctggata ggcacacaga cccaagccgc gacaattcgc ccatttacaa attgaaggat
      901 catccatggt tgggcgtgtc tttcaatttg ggcagcgaga ataccgtcaa aaatggcaaa
      961 tcatccagca aattgatatc ttcttttaat gaaaacaata ataatcaaac catcgtctct
     1021 acgacagaag gtgaccctat ttcccttggc gaccagcagc gcgaacatac cgccgtggtc
     1081 tattatctga acgccaaact gcacctgctg gacaaaaaag ggattaaaga tatcaccgac
     1141 aaaacagtgc agttgggtgt cttgaggccg agcatcgatg tgcggctgca aagaaatacg
     1201 gggcttgccg gcttgctaaa tttttgggct agttgggaca ttaaagataa cgggcagatt
     1261 ccggtcaagc tcggcctgcc ggaagtcaaa gcaggccgct gcatcaacgc aaataacccc
     1321 aataagagta ccaaagccc
//
LOCUS       DAWKQJ010000231         1311 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000231 DAWKQJ010000000
VERSION     DAWKQJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.231"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(101..1024)
                     /locus_tag="WJL20_001965"
     CDS             complement(101..1024)
                     /locus_tag="WJL20_001965"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ7684066.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          348 a          313 c          330 g          320 t
ORIGIN      
        1 cgtgggaatg acgaatttta ggttgctgtt ttggtttttt gtccttgtag gaatgatgaa
       61 attttaagtt ttaggaattt accggaaaaa acagaaagcg ttatccacaa gttctgatgt
      121 tcagctcgtg aaatgcgtcg ggcaaatcat cgctgtcggc aaattccacc cggtcgtaag
      181 ccgtttcgtc tgccaaaacc gcgcgcaaga gtgcgttgtt gatggcgtgt cccgatttgt
      241 agccttcaaa cgcgccgaca atcggatgtc cgacgatata caaatcaccg atggcatcaa
      301 ggattttgtg gcgcacaaac tcatcgggat agcgcaagcc ttcaggattc aggacatccg
      361 tgtcgtcaat cacgatggcg ttgttcaaat tgccgcccaa acccagattg tgggcgcgca
      421 tcatttccac ttcgtgcata aagccgaaag tgcgcgcgcg cgcgatttcg tcgatgtagg
      481 atttgccggc gaaatcaatt tcaaaagtgg gcgagctgcg gttgaaaacc ggatggtcga
      541 attcgatggt cagcgttacc ttaaagccgt catacggcgt aaagcgcacc catttgcccg
      601 cttctttgat ttcgacaggc ttgaggattt tcaaaaaacg cttttgcgcc ttttgatcga
      661 ccacgcccgc atcttgcaaa aggtaaataa acggcaggct ggagccgtcc ataatcggga
      721 tttcgggcgc gttcagctca atcagcgcgt tgtcgatacc gtaggcggac agcgcggaca
      781 taatgtgttc gattgtgccg acgcgcacgc ctttgtcggt aacgatggtg gaggaaaggc
      841 gggtatcgtt gatcaaataa ggggtcagtt tgatttgttc gcccatctcg ccgtccaaat
      901 cggtgcgtcg gaaggaaatc ccgctgtttt caggcgcggg gtgcagggtc agcgcgacgc
      961 gttcgcctga atgcaacccg actccggtaa cgctgatgga tttcgccaaa gttctttgca
     1021 gcataaaccg ctcccttatc aatggggtaa gttttggaat aatacgataa aaccggaaaa
     1081 acaagctatg tttttccata gtatttgcca atgtatccgt tttcaatacg taagccgcat
     1141 aaaaatgtat agtggattaa caaaaatcaa gacaaggcga cgaacccgcc tccctcctga
     1201 aaaacgcaaa aaatgccgtc cgaaaacctt tcggacggca ttttcgcgtg aaccgtcatt
     1261 ctcacaagga cataaaacca aaacaggaaa ccaaaaacag caacctaaaa t
//
LOCUS       DAWKQJ010000232         1299 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000232 DAWKQJ010000000
VERSION     DAWKQJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.232"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(205..468)
                     /locus_tag="WJL20_001966"
     CDS             complement(205..468)
                     /locus_tag="WJL20_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684067.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(487..1218)
                     /locus_tag="WJL20_001967"
     CDS             complement(487..1218)
                     /locus_tag="WJL20_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ7684068.1"
                     /translation="MSATEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVK
                     NNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKI
                     SRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNR
                     IKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIG
                     TKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          358 a          321 c          205 g          415 t
ORIGIN      
        1 ctgtaattgc tcttacctac cttgatgccg ataaagcggc ggctttttta gacatccaac
       61 tcgtgcttgt gaatgccttc ggctgccagc agttgcattg aggccgtctg aaaaacggat
      121 attcagacgg cctattgcta ctttaaataa tatttgggtt taaaatatca tcgtatttcc
      181 caaattcggg gaacagaaaa tcaattattt aaatttttca tcctcataat atgggtgatc
      241 aaaaagctct ccttcccaca tatttatttc ttctatgcct ttatctttca taaactcatg
      301 ataatcataa aaatcttttc catattgtcc gtaaaaaatt ttcatatccc caagccacat
      361 agcataacat tcatcgcata aaatgacctc cccagagaaa tatttaggct ttgcatgata
      421 aaggtagcct tgctcacatc ttgggcaaat tttaatttct ttatccataa gagattcctt
      481 tcttaattat ttctgaggat atgcagaaat gattttattt gttccttttt gcacaacaat
      541 ccgcactttt gtttctcctt ttgttccaat aggtttcttc atatctacaa gatatacgga
      601 tgaatcattt ggcaaaggat ttcctttcat tctccaagct tcatcaacca aaggcaaaac
      661 ttctttacgg gacacattaa aaacagaatg aacaggtttg tttggatttg gaattgtatg
      721 ggctaaaaca tgtttaatcc tattaccatg cttagcatct aacccataat ctaaccccgc
      781 cggtgttgtc caaaccttat taaccttatc ataagtaagc ttgattccgg tctgcttctg
      841 aatttcctgt atggctttag ccgtatcaac tgcttcgtct cccgccttca gaacataagc
      901 cccgcctcgg ctgattttag tcgcacctgc cggaacaaag ggaacggcag ctgcggcggc
      961 atccacacca aaatcaatcg ccgcttctcg agccatctgc ttatcgccaa tagatttggc
     1021 gtaaatccat ttaccgccgt cccatacgag gttgccgata tcccaaataa tatctaatgc
     1081 attattttta acagctgttt cggaagcatt tgccgccaca tcaacattgc ctcctgtaac
     1141 acctacagtc gctccggcta ccaattttgc atacgcagta atattcgctt tagctttttc
     1201 gatttcggtc gcactcatac gactgaaatc agtattctta accaaagcct caccaacaat
     1261 ctcaccgact gcagcgccta tcgcaccatc ctgacactt
//
LOCUS       DAWKQJ010000233         1272 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000233 DAWKQJ010000000
VERSION     DAWKQJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.233"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..890
                     /locus_tag="WJL20_001968"
     CDS             <1..890
                     /locus_tag="WJL20_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7684069.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          351 a          373 c          328 g          220 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagagg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaattccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctagcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggagtttaaa ggactggatt cccgccgtcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cc
//
LOCUS       DAWKQJ010000234         1260 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000234 DAWKQJ010000000
VERSION     DAWKQJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.234"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            236..1189
                     /locus_tag="WJL20_001969"
     CDS             236..1189
                     /locus_tag="WJL20_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ7684070.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          275 c          433 g          352 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg tgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gccccgcctt
//
LOCUS       DAWKQJ010000235         1238 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000235 DAWKQJ010000000
VERSION     DAWKQJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.235"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            311..>1238
                     /locus_tag="WJL20_001970"
     CDS             311..>1238
                     /locus_tag="WJL20_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7684071.1"
                     /translation="MNAQNLPEVKWGNDYNKLVQKINEREVTHTSFFRIKQSVSFSFN
                     NKDEVVAEKKDAVVFGAATYLPPYGKVSGFDEKRLKERGNALDWIGTTKPGLVGYSYE
                     GSTCSSGGCPTVAYRTQFTFGNSSLAKKTNGGGLDIYEDKSRDNSPIYKLKDHPWLGV
                     SFNLGGESSFKPKRNGSLVSSFSEDVTQQNGAGSQHKDKNLVYTTDDYKSQNNKNHQD
                     KHHAVAFYLNAKLHLLDKKHIKNIVQGKTVNLGILKTRIEPTEAWKRRNSNFFNGSWT
                     YEEKGTVSVKLKLPEVKAARCINAAHPNQKARD"
BASE COUNT          382 a          276 c          314 g          266 t
ORIGIN      
        1 tgtcgggcat ctgcagcggt ttgctaaaaa acgctttacc gtgatcagtg tgcaaagtta
       61 aaatggggag gtaagctttt caaccagcaa ttcggcgggc gcggaatcgg gcggtttacc
      121 gaaccccggc gttcgcggcg cgccgccccg tcccgtgaag gcaaactcaa ggaataaaag
      181 atgaataaaa cttggaaacg gcaggttttc cgccataccg cgctttatac cgccatattg
      241 atgttttccc ataccggcgg ggggggggca ggcgcaagcg caaacgcaaa cgcataaata
      301 cgctattgta atgaacgcgc aaaatctgcc tgaggtaaag tgggggaatg actataacaa
      361 attggtgcaa aaaatcaatg aacgcgaagt tacccatacg agttttttca gaatcaaaca
      421 aagcgttagt ttctcattca ataataaaga tgaagttgtt gctgaaaaaa aagatgctgt
      481 cgttttcggc gcggcgacct acctgccgcc ctacggcaag gtttccggtt ttgatgaaaa
      541 gaggctgaaa gagcgcggca atgcccttga ttggattggt acgaccaaac cggggctggt
      601 aggctacagc tacgaaggta gcacatgctc cagcggaggt tgtcctacag ttgcctatag
      661 aacccaattt accttcggca attccagttt ggcaaaaaag acaaacggcg gcgggctgga
      721 tatatacgaa gacaaaagcc gcgacaattc gcccatttac aaattgaagg atcatccttg
      781 gttgggcgtg tctttcaatt tgggcggaga gagctctttc aaaccaaaga gaaacggttc
      841 tttggtatct tcttttagcg aggacgtgac gcagcaaaat ggtgcgggca gccaacacaa
      901 agacaaaaac ctcgtttata cgacagacga ttacaagagt cagaataata aaaaccatca
      961 ggacaaacac cacgccgtcg ccttttatct gaacgccaag ctgcacctgc tggataaaaa
     1021 acacattaaa aatatcgtgc aaggtaaaac agttaatttg ggtatcttga aaacacgcat
     1081 cgagccgacg gaagcatgga aaagacggaa tagtaacttt tttaacggta gttggacgta
     1141 tgaagagaaa ggaacagtca gcgtcaaact caaattgccg gaagtcaaag ccgcgcgttg
     1201 catcaacgcg gcgcatccga atcaaaaagc ccgtgatt
//
LOCUS       DAWKQJ010000236         1233 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000236 DAWKQJ010000000
VERSION     DAWKQJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.236"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..373
                     /locus_tag="WJL20_001971"
     CDS             41..373
                     /locus_tag="WJL20_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ7684072.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGTESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            705..1214
                     /locus_tag="WJL20_001972"
     CDS             705..1214
                     /locus_tag="WJL20_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ7684073.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          332 a          231 c          291 g          379 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggca cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg aagcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggt gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggaaattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tag
//
LOCUS       DAWKQJ010000237         1226 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000237 DAWKQJ010000000
VERSION     DAWKQJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.237"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..645
                     /locus_tag="WJL20_001973"
     CDS             <1..645
                     /locus_tag="WJL20_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7684074.1"
                     /translation="GKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPV
                     GALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAG
                     LLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDG
                     RLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            664..1038
                     /locus_tag="WJL20_001974"
     CDS             664..1038
                     /locus_tag="WJL20_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ7684075.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          399 a          229 c          271 g          327 t
ORIGIN      
        1 ggtaaagcag ctatcagcac ctgtatggct aatccttctg gttgtactgt catggttacg
       61 caggctgctg aagcgggcgc gggaatcgcc acgggtgcgg taacggtagg caacgcttgg
      121 gaagctccag taggagcgtt gtcgaaagcg aaggcggcta agcaagctgc tcctaaagaa
      181 acaataaaca atttggcaaa tttagccaaa gcagaacagc agattttatt ccgtattgcc
      241 caacgcgata cgcaactgga tgcatggaag acgggattta acaatagagt aaggaaagga
      301 gcaggcttgc ttgatgcaag taatattccg ataaccatta acggaaaaac catcaaacct
      361 gtacaagcca taagcttaaa gggagcaccc gtttacagcg gcgtaagcga acaggagatt
      421 tttgcgcttt atcggcagat gactggccag aatccgaatt ttagagtttt gcctgacgga
      481 agattagcaa atggcattat cagtactgga gaatgggcag gaacaaaaat tgcattaaga
      541 aatttttcaa aaacagagaa ttcaactcaa gcacgatgga cattagattt gcagaatcct
      601 ccatcattta ttaaaggtac taaattggag cttaaattcc aataatttac aaaggatttt
      661 accgtggatg agaaacaaaa aattaagatt cttgattttc aaatcgattt atcctcaatt
      721 tttaactctt ataaaaatca aatgggtatt aatattcaag ataaaaactt aaaaaacaat
      781 ttctgttctt ttatggaaga actgttaaat gacggttcaa tccgtttaca tgattatacc
      841 gacggtatcg gaattcctct aactggaact tcaaaagaac aagtgcagaa attgaaagac
      901 atatggccta ctttggaaga tgcccaagca atatggcctg aagacccttg gtattactta
      961 gaatggcttt ggtgggatat tgcgtgtcca atagatttgg ccgatttgcc gaatattgat
     1021 atttatgagc aagcgtaggt atggttagcc gcctttagcg gcgtaaccgt acgcatatca
     1081 gcaaacttta taaaataaca aggccgtctg aaatctgttt ttcaactttt tcagacggcc
     1141 ttgcaacttg gcatttcatt cgtacggtta cgccgctaaa ggcggctaac cgtacctacg
     1201 agctctgata aaaatgattt atggaa
//
LOCUS       DAWKQJ010000238         1152 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000238 DAWKQJ010000000
VERSION     DAWKQJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.238"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..236
                     /locus_tag="WJL20_001975"
     CDS             <1..236
                     /locus_tag="WJL20_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684076.1"
                     /translation="FYYIKKGENPAIITHRNYHRYGENDYSTSVGSCINGFTVRYYPF
                     IREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            253..>1152
                     /locus_tag="WJL20_001976"
     CDS             253..>1152
                     /locus_tag="WJL20_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684077.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIET"
BASE COUNT          380 a          171 c          271 g          330 t
ORIGIN      
        1 aattttacta tattaaaaaa ggagaaaatc cggcgattat tactcatcgg aactatcata
       61 ggtatggaga gaacgattac agcactagcg taggttcctg tattaacggt ttcacggtac
      121 ggtattaccc gtttattcgg gaaaagcagc agctcacaca gcaggagttg gtaggttatc
      181 accaacaagt agagcaattg gtacagagtt ttgtaaacaa tccaagtaaa aaataattta
      241 aaggatctta ttatgaatga gggtgaagtt gttttaacac cagaacaaat ccaaaccttg
      301 cgtggttatg ctttccgtgg cgatacctat ggcggttggc gttatttggc taatttgggt
      361 gaccgttatg cggatgatgc tgctgcaatt gtcggtaagg atgcaaactt aaatggtttg
      421 aatttatgga tgaaaaaagg tgtggaaaac ctatgggatg atacggtcgg taaaaagacc
      481 cgtttagaga aatttgatcg ggttgcattg caacatttca gccaatatgt agatctaatt
      541 aatgaaaata atggtagatt acctaacact agtgaaattg agagaagtta ctataaagcc
      601 gttaccgaaa atggtgtttc ttctagtgca gctattgatt tagttattaa tcgctcactt
      661 ccggatatgg cagatggtta ttgggcatta ggtttgggga tagaagccga acgtatccac
      721 aatgcgcaag cagtaaataa tccgaacggt agcgaatggg ataatagaaa gcagttaata
      781 tctgctttag ataaaggatt tgatggatct tttaaagaga agcattttac ttttttacaa
      841 tctgtgataa tggatgtaac aaagttaggt gttgaatata caatagatgg ttggcaaaaa
      901 attggaggtt ggggtaatgg gataatcaat gatttatata aaagtgttgc aaaaagagag
      961 tggactggaa tatttgagat cgttaataat aacatcaagc aatttagaga tctgttccca
     1021 aatccggaag gctggatcga tgatggtcac caatgtttcg ctccttgggt taaagaaact
     1081 aaaaaacgca atggcaaata tcatgtctac gacccccttg ccctagacct agatggcgac
     1141 ggtatagaaa cc
//
LOCUS       DAWKQJ010000239         1112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000239 DAWKQJ010000000
VERSION     DAWKQJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.239"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1112
                     /locus_tag="WJL20_001977"
     CDS             <1..>1112
                     /locus_tag="WJL20_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7684078.1"
                     /translation="QVQLAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVT
                     AAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGT
                     SDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNA
                     GISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAG
                     CVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAAL
                     NGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGA
                     MTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWT"
BASE COUNT          321 a          262 c          300 g          229 t
ORIGIN      
        1 caggtgcagc tggcctacga caaatgggac tataaacagg aaggcttgac cggagccggt
       61 gcagcgattg tggtgattat tgtaaccgct ctaacttatg gatacggagc ggctgcagcg
      121 ggtagcgtaa ctgccgcagg aagtagtaca gccgcagctg caacaacgac agcggcagca
      181 actaccactg tttctactgc agctgccatg caaaccgcag ctttagcctc cttgtatagc
      241 caagcagctg tagccatcat caataataaa ggcgatgtag gcaaagcatt aaaagatctc
      301 ggcaccagtg atacggtcaa gcagattgtt acttccgcac tgacggcggg tgcattaaac
      361 cagatgggcg cagatattgc ccaattgaac agcaaggtaa gaaccgaact gttcagcagt
      421 acgggcaatc aaaccattgc caaccttgga ggcagattgg ccaccaacct cagcaatgca
      481 ggtatctcag ctggtatcaa taccgccgtt aacggcggca gcttgaaaga caacttaggc
      541 aatgccgcat tgggagcatt ggttaatagc ttccaagggg aagccgccag caaaatcaaa
      601 acaaccttca gcgacgatta tgttgccaaa cagttcgccc acgctttggc gggttgtgtt
      661 agcggactgg tacagggaaa atgtaaagat ggggcaattg gcgcagcagt tggggaaatc
      721 gtagctgaat ccatacttgg cggcagaaac cctgctacac tcagcgatgc agaaaagcat
      781 aaagttatca gttactcgaa gattattgcg ggcagcgtgg cggcactcaa cggcggcgat
      841 gtgaatactg cggcgaatgc ggctgaggta gctgtggtga ataatgcttt gaattttgac
      901 agtaccccta ccaatgcgaa aaagcatcaa ccgcagaagc ccgacaaaac cgcactggaa
      961 aaaattatcc aaggtattat gcctgcacat gcagcaggtg cgatgactaa tccgcaggat
     1021 aaggatgctg ccatttggat aagcaatatc cgtaatggca tcacaggccc gattgtgatt
     1081 accagctatg gggtttatgc agcaggttgg ac
//
LOCUS       DAWKQJ010000240         1112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000240 DAWKQJ010000000
VERSION     DAWKQJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.240"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            153..1052
                     /locus_tag="WJL20_001978"
     CDS             153..1052
                     /locus_tag="WJL20_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7684079.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          188 a          255 c          355 g          314 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagccgacc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggt agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWKQJ010000241         1063 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000241 DAWKQJ010000000
VERSION     DAWKQJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.241"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..551)
                     /locus_tag="WJL20_001979"
     CDS             complement(123..551)
                     /locus_tag="WJL20_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ7684080.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYLPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="WJL20_001980"
     CDS             complement(532..966)
                     /locus_tag="WJL20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ7684081.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLCRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
BASE COUNT          273 a          316 c          192 g          282 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcatccc
       61 ctgcgaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggagatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac tcgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc aacattgccg ctgatgatgt ccgacacaga tgcggtttgc cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 agaaacatcc atatccgcac acgaccgctt atccgtcaca cgctccaaca ccgcgcgata
      901 ggtaccgtca tccaagccca gctccttttg agcaatctta attttcgcaa tcaacgcccg
      961 gcgcattatc tctccaatac aacatatagt ataaattagc gcattttata caataaaata
     1021 caatatatag tattaagccg atgttttttt gcgaaacgga cag
//
LOCUS       DAWKQJ010000242         1058 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000242 DAWKQJ010000000
VERSION     DAWKQJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.242"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..513
                     /locus_tag="WJL20_001981"
     CDS             <1..513
                     /locus_tag="WJL20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7684082.1"
                     /translation="RWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVASGRL
                     PQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVL
                     EIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQETGWETKRSLAQMMEDSWRW
                     VSRNPNGYGD"
     gene            514..>1058
                     /locus_tag="WJL20_001982"
     CDS             514..>1058
                     /locus_tag="WJL20_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7684083.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKREAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRT"
BASE COUNT          236 a          316 c          274 g          232 t
ORIGIN      
        1 cgttggagcg tgattttgtt gcgttatttc aacccgatcg gcgcgcatga aagcggcttg
       61 atcggcgaac agcccaacgg cattcccaac aaccttttgc cctatatctg ccaagtggct
      121 tcgggcaggc tgccgcaact gtcggtattc ggcggcgatt atccgacccc ggacggtacg
      181 ggtatgcgcg actatattca tgtgatggat ttggcagaag ggcatatcgc ggcaatgaag
      241 gcgaaaggcg gtgttgccgg cgtacatttg ttcaacttgg gttcggggcg cgcctattcg
      301 gttttggaaa tcatccgtgc ctttgaagcc gcatccggct taaatattcc ctatcaaatc
      361 caaccccgac gcgccggaga cttggcgtgt tcctttgccg acccgtccca taccaaacaa
      421 gagacaggtt gggaaaccaa acgcagcctt gcgcaaatga tggaggattc gtggcgttgg
      481 gtcagccgca accccaacgg atatggggat taaatgcaaa ccgcaaacaa aaaaaccatc
      541 ctcgttaccg gcggcgcggg ctttatcggc tctgccgttg tccgccatat cattcaaaac
      601 acccaagatt ccgtcgtcaa cctcgacaaa ctgacctacg cgggcaatct cgaatcgctg
      661 accgacattg ccgataatcc ccgctatgct ttcgagcaag tggacatttg cgaccgcgcc
      721 gaactcgacc gcgtattcgc acaacaccgg cccgatgccg tgatgcactt ggcggcggaa
      781 agccatgtcg accgctctat cggttcggca ggcgagttta tccaaaccaa tatcgtcggc
      841 acattcaacc tacttgaagc tgcgcgtgcc taccggcagc aaatgccgtc tgaaaagcgc
      901 gaagctttcc gtttccacca tatttccacc gatgaagttt atggcgattt aggcggcacg
      961 gacgatttgt ttaccgaaac cgcgccctac gcgccgtcca gcccctactc tgcctctaaa
     1021 gcgtccagcg accacctcgt ccgcgcgtgg ttgcgtac
//
LOCUS       DAWKQJ010000243         1017 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000243 DAWKQJ010000000
VERSION     DAWKQJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.243"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..696
                     /locus_tag="WJL20_001983"
     CDS             <1..696
                     /locus_tag="WJL20_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7684084.1"
                     /translation="PGLSAHPYLSGVEKACFMSHTVLWKQALDESLPYIAVFEDDVLL
                     GEGAEKFLAEDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHW
                     GTAGYIISREAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQEL
                     HYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAE
                     LEKAYGRRVISFK"
BASE COUNT          276 a          194 c          270 g          277 t
ORIGIN      
        1 cccggtttgt cggcgcaccc ctatttgagc ggagtggaaa aagcctgctt tatgagccac
       61 accgtattgt ggaagcaggc gctggacgaa agcttaccgt atattgccgt atttgaggac
      121 gacgttttac tcggcgaagg cgcggagaaa ttccttgccg aagatacttg gttggaagag
      181 cgttttgaca aggattccgc ctttatcgtc cgcttggaaa cgatgtttgc gaaagttatt
      241 gtcagaccgg ataaagtcct gaattatgaa aaccggtcat ttcctttgct ggagagcgaa
      301 cattggggga cggctggcta tatcatttcg cgtgaggcga tgcggttttt cttggaaagg
      361 tttgccgttt tgccggcaga gtggattaaa gcggtagatt ggatgatgtt tacttatttc
      421 tttgataagg aggggatgcc tgtttatcag gttaatcccg ccttatgtac ccaagaattg
      481 cattatgcca agttcctcag taaaaacagt atgttgggta gcgatttgga aaaagatagg
      541 gaacaaggaa gaagacaccg ccgttcgttg aaggtgatgt ttgacttgaa gcgtgctttg
      601 ggtaaattcg gtagagaaaa gaagaaaaga atggagcgtc aaaggcaggc ggagcttgag
      661 aaagcttacg gcaggcgggt catatcgttc aaatagtttg tgtaaaatat agcggattaa
      721 aattagaaat ggatacactg tcattcccgc gcaggcggga atctagtccg ttgagtttca
      781 gtcatttccg ataaatcacc gcaacgttaa atttctagat tcccgctttt gcgggaatga
      841 cggcggggga tttctgtttt tccgacaaat tcctaaaact taaagttccg ttattcccgc
      901 gaaaacagaa aaccaaaaac agaaacctga aaacagaaac ctaaaattcg tcattcccgc
      961 gcaggcggga atctagtccg ttcggtttcg cttgttttaa gtttcgggta acttcca
//
LOCUS       DAWKQJ010000244         1007 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000244 DAWKQJ010000000
VERSION     DAWKQJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.244"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1007
                     /locus_tag="WJL20_001984"
     CDS             <1..>1007
                     /locus_tag="WJL20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684085.1"
                     /translation="ERNGKKHVFMFGAMGFGGRGAYALDLSKIDSGNGNLADVSLFDV
                     KHDKNGNNGVKLGYTVGTPQIGKTHNDKYAAFLASGYATKTIDDQQNKTALYVYDLES
                     SGTLIKKIDVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGSMYRFDLSNQDPNQWSV
                     RAIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEEDVDKKDEQYIYGIFDDDTVANN
                     VNVKLSGLGGGLLEQELKQEGKTLFLTDYKRSDGSGNKGWVVKLKDGQRVTVKPTVVL
                     RTAFVTIHKYTGTDKCGAETAILGINTADGGKLTKKSARPIVPADNTAVAQYSGHKKG
                     T"
BASE COUNT          283 a          246 c          276 g          202 t
ORIGIN      
        1 gaacggaacg ggaaaaaaca cgtgtttatg ttcggcgcga tgggttttgg cggcagaggc
       61 gcgtatgcct tggatttaag caaaatcgac agcggcaacg gcaacctggc agacgtttcc
      121 ctgtttgatg tcaaacatga caagaatggc aataacggcg tgaaattagg ctacaccgtc
      181 ggcacgccgc aaatcggcaa aacccacaac gacaaatacg ccgccttcct cgcctccggt
      241 tatgcgacta aaaccattga cgaccaacaa aataaaaccg cgctgtatgt gtatgatttg
      301 gaaagcagcg gtacgctgat taaaaaaatc gatgtacccg gcggcaaggg cgggctttcg
      361 tcccccacgc tggtggataa agatttggac ggcacggtcg atatcgccta tgctggcgat
      421 cgcggcggca gtatgtaccg ctttgatttg agcaatcaag atcctaatca atggtctgta
      481 cgcgccattt ttgaaggcac aaaaccgatt acttccgcgc ccgccatttc ccaactgaaa
      541 gacaaacgcg tggttatctt cggcacgggc agcgatttga gtgaagagga tgtggacaaa
      601 aaggacgaac aatatattta cggtatcttt gacgacgata cggtggcgaa taacgtaaat
      661 gtaaaactca gcggtttggg aggcgggctg ctcgagcaag agcttaagca ggagggtaaa
      721 accttattcc tgaccgatta taagcgatcc gacggctcgg gcaataaggg ctgggtagtg
      781 aaattgaagg acggacagcg cgttaccgtc aaaccgaccg tggtattgcg taccgccttt
      841 gtaaccatcc ataaatatac gggtacggac aaatgcggcg cggaaaccgc cattttgggc
      901 atcaataccg ccgacggcgg caagctgacc aagaaaagcg cgcgcccgat tgtgccggcc
      961 gacaatacgg ctgtcgcgca atattccggc cataagaaag gcaccaa
//
LOCUS       DAWKQJ010000245          998 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000245 DAWKQJ010000000
VERSION     DAWKQJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.245"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>998
                     /locus_tag="WJL20_001985"
     CDS             <1..>998
                     /locus_tag="WJL20_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684086.1"
                     /translation="NLNGKDHVFMFGAMGFGGRGAYALDLTKADGSDPTAVSLFDVKH
                     DNNGNNSNNSVQLGYTVGTPQIGKTHDGKYAAFLASGYATKTIDDQQNKTALYVYDLE
                     SSGTLIKKIDVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGKMYRFDLSGQDPQQWS
                     VRTIFSGNKPITSAPAVSQLKDKRVVIFGTGSDLSEEDVDNNDIQSIYGIFDNDTDTG
                     TAQDGQGKGLLEQKLSEENKTLFLTDYKRSDGSGDKGWVVKLQPGQRVTVKPTVVLRT
                     AFVTIRKYNDGGCGAETAILGINTADGGKLTKKSARPIVPEANTAVAQYSGHKQTT"
BASE COUNT          274 a          257 c          265 g          202 t
ORIGIN      
        1 aacttaaacg gtaaagacca tgtgtttatg ttcggcgcga tgggctttgg cggcagaggc
       61 gcatacgcct tggatttgac caaagccgac ggcagtgacc cgaccgccgt ttccctgttt
      121 gatgtcaaac atgacaataa tggcaataat agcaataata gcgtgcaatt aggctacacc
      181 gtcggcacgc cgcaaatcgg caaaacccac gacggcaaat acgccgcttt cctcgcctcc
      241 ggttatgcga ctaaaaccat tgacgaccaa caaaataaaa ccgcgctgta tgtgtatgat
      301 ttggaaagca gcggtacgct gattaaaaaa atcgatgtac ccggcggcaa gggcgggctt
      361 tcgtccccca cgttggtgga taaagatttg gacggcacgg tcgatatcgc ctatgccggc
      421 gatcgcggcg ggaaaatgta ccgctttgat ttaagcggtc aagatcctca acaatggtct
      481 gtacgcacca ttttcagcgg caacaaaccg attacttccg cgcccgccgt ttcccaactg
      541 aaagacaaac gcgtggtcat cttcggcacg ggcagtgatt tgagtgagga agatgttgat
      601 aataatgata tacaatctat ttacggtatt tttgacaatg acacagacac gggtacggcg
      661 caagacgggc agggcaaagg gctgctcgag caaaagctta gtgaggaaaa taaaacctta
      721 ttcctgaccg attataagcg atccgacggc tcgggcgaca agggctgggt agtgaaattg
      781 cagcccggac agcgcgttac cgtcaaaccg accgtggtat tgcgtaccgc ctttgtaacc
      841 atccgcaaat ataacgacgg cggctgcggc gcggaaaccg ccattttggg catcaatacc
      901 gccgacggcg gcaagctgac gaagaaaagc gcgcgcccga ttgtgccgga agccaatacg
      961 gctgtcgcgc aatattccgg ccataagcaa accaccaa
//
LOCUS       DAWKQJ010000246          979 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000246 DAWKQJ010000000
VERSION     DAWKQJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.246"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..411)
                     /locus_tag="WJL20_001986"
     CDS             complement(<1..411)
                     /locus_tag="WJL20_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684087.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDS"
     gene            complement(408..599)
                     /locus_tag="WJL20_001987"
     CDS             complement(408..599)
                     /locus_tag="WJL20_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684088.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
BASE COUNT          259 a          238 c          194 g          288 t
ORIGIN      
        1 actgtctttt tcgcgccgtt gcttttccaa ggtttgcgct ttacctctgt tgattcctgc
       61 aaaatatccc aaagctaccg ccccgccgat tttgacaatt tcaatagcga cgctgtcctt
      121 atcgcgccat aacgcagtaa tcagcacggc ggcaatcaga aaagaaatag caatcacggc
      181 attcagacgg cgtttgtgta cctctttgaa aatctcacca tgttgctgtt caccctgttt
      241 ttgcgcttcg atggtagcca gtgcaatgcg ttcgtgagat tggatttcgt ccttacggct
      301 ttccaactcc ttctgtttga ctgcaatatc ctgtttttga agttctaaaa actcctgaag
      361 cattttctgc tgctcatcgg ccggcagatt ctcggatttt tccccgctca taaatcaacc
      421 gccactaagg ttttgtaacg gactttgaat ttgccgtaac cccgagcccc tattttcggc
      481 ggcacaaatt ccacggacgc aatattgccc gtcttacggt ctttacggac ttcttccaaa
      541 taatcggcag cattgagcag ccgttcgcgg actgccgtat attctttgac ggtattcatt
      601 tttcaacctc gcccaaatag tgcaaatatt agcatatggg atttattttt caagaaaatc
      661 aaatccatat aaaacaaccg tataatattt taatactata tatggtaaat ttcttctaaa
      721 aatacacact aaaataccat atcttgataa ccgcaacgcc gcacaaatgg cagggcattg
      781 atgcccgaca acaaaaaatg ccgtctgaaa cagcattcga gctgttttca gacggcattt
      841 ttctttgatt ttgttgggga aagggttgac ttttaacgtg tccacgttta taattcaatt
      901 catcggcgta gtgagggccg atacgggaaa agccccaccg aagcggggct caactgggag
      961 aaagaagatg aataaacgc
//
LOCUS       DAWKQJ010000247          978 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000247 DAWKQJ010000000
VERSION     DAWKQJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.247"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            9..>978
                     /locus_tag="WJL20_001988"
     CDS             9..>978
                     /locus_tag="WJL20_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ7684089.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          253 g          205 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWKQJ010000248          970 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000248 DAWKQJ010000000
VERSION     DAWKQJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.248"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..619)
                     /locus_tag="WJL20_001989"
     CDS             complement(53..619)
                     /locus_tag="WJL20_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ7684090.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          257 a          295 c          219 g          199 t
ORIGIN      
        1 aaggcgaggc aacgacgtac tggtttttgt taatccacta tattttccga catcattgaa
       61 tcaaacccaa gtgcgacaag agcgtccatg taccgatggc aatcaacacc aaacctccgg
      121 caaactcggc gcgtttgccg aacaatacgc ccaaagccct tcccgccgtc agcccgaccg
      181 taaccatcac cgtcgtcgcc atgccgatga cggcggcggc aaaggcgatg tttacctcca
      241 taaacgccaa gcccaccccg acgatcatag agtcgatgct ggttccaaaa gcagtcaaaa
      301 ccgtcatcca taggctttcc cgtttgcttt cgcgtacatc ttccgccttg ccggacagcc
      361 cttcgcgcat cattttcaga cccagcccgc ccagcaggac gaaagccgcc caatggtccc
      421 attcgctgat aaacggtttg gcataaaaac cgcctaccca gcctgccagc ggcgtgagtg
      481 cctcaaccgt gccgaacacc aaggctgttg ctgcaatttt gcgcggaggc atcctgaccg
      541 ccgcaccctt tgccagcgcg acggcaaacg catccatcga catccccagc gcaatcaaga
      601 gcaaagcgta aagactcata ccgcacccgt ccttaaaaag ggcggattat agcaaaacag
      661 caaaacgcga aaaacgccgc acgaaaaccc acatcccgtc attcccgcaa aaacaaaaaa
      721 tcaaaaacag aaatcccgtc attcccgcgc aggcgggaat ccagagttgt cggtgcggaa
      781 acttatcggg aaaaacggtt tctttagatt ttacgttcta gattcccact ttcgtgggaa
      841 tgacgggatg taggtttccg tgcggatgga ttcgtcattc ccgcgcaggc gggaatccaa
      901 agttgtcggt gcagaaactt attggataaa acggtttctt tagattttac gttctagatt
      961 cccgctttcg
//
LOCUS       DAWKQJ010000249          954 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000249 DAWKQJ010000000
VERSION     DAWKQJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.249"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WJL20_001990"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WJL20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ7684091.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          220 a          206 c          245 g          283 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 tatcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata atggcgtttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa caggcataac
      901 ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta tatc
//
LOCUS       DAWKQJ010000250          947 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000250 DAWKQJ010000000
VERSION     DAWKQJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.250"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            3..>947
                     /locus_tag="WJL20_001991"
     CDS             3..>947
                     /locus_tag="WJL20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ7684092.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQGQQYSAEKAQRQSQTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHH
                     QITLHHSTIYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVVIENRPA
                     IVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRALK
                     AHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPK
                     QTDFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNL"
BASE COUNT          305 a          292 c          196 g          154 t
ORIGIN      
        1 ccatgagcta cacgcaactg acccaaggcg aacgatacca catccaatac ctgtcccgcc
       61 actgcaccgt caccgaaatc gccaaacagc tgaaccgcca caaaagcacc atcagccgcg
      121 aaatcagacg gcaccgcacc caagggcagc aatacagcgc cgaaaaagcc cagcggcaaa
      181 gccagactat caaacagcgt aagcgacaac cctataagct cgattcgcag ctgattcagc
      241 acatcgacac ccttatccgc cgcaaactca gtcccgaaca agtatgcgcc tacctgtgca
      301 aacaccacca gatcacgctc caccacagca ccatttaccg ctaccttcgc caagacaaaa
      361 gcaacggcag cacgttgtgg caacatctca gaatatgcag caaaccctac cgcaaacgct
      421 acggcagcac atggaccaga ggcaaagtcc ccaaccgtgt cgtcatagaa aaccgacccg
      481 ctatcgtcga ccagaaatcc cgtatcggcg attgggaagc cgacaccatt gtcggcaaag
      541 gacagaaaag cgcattattg accttggtcg aacgcgttac ccgctacacc atcatctgca
      601 aattggatag cctcaaagcc gaagacactg cccgggcagc tgttagggca ttaaaggcac
      661 ataaagacag ggtgcacacc attaccatgg ataacggcaa agagttctac caacacacca
      721 aaataaccaa agcattgaaa gcggagactt atttttgtcg cccttaccat tcttgggaga
      781 aagggctgaa tgagaacacc aacggactca tccggcaata cttccccaaa caaaccgatt
      841 tccgtaacat cagtgatcgg gagatacgca gggttcaaga tgagttgaac caccgaccaa
      901 gaaaaacact tggctacgaa acgccaagtg ttttattctt gaatctg
//
LOCUS       DAWKQJ010000251          933 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000251 DAWKQJ010000000
VERSION     DAWKQJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.251"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(9..200)
                     /locus_tag="WJL20_001992"
     CDS             complement(9..200)
                     /locus_tag="WJL20_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684093.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            329..889
                     /locus_tag="WJL20_001993"
     CDS             329..889
                     /locus_tag="WJL20_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ7684094.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          260 a          262 c          186 g          225 t
ORIGIN      
        1 ggtttttgtt aatccgctat accacgatga atccttcgca atatctgttt atcgacctca
       61 attttgacaa aataccggat acgcgccttt gttgcttttc catcttccaa ccaactgtaa
      121 atctcaaaca gccggtacac gccatgcttc agtttctttt cctgtcggcg gattgtttcg
      181 acaaagaatt gaaaatccat ttcatgcacc ttaaaattta atctgcattc aaaccttttc
      241 actttggaag caccatttat cggatgtccc ttcgcaataa acaaattttc ccgataccgc
      301 cgcccatttc aacccaaacc caaaacctat gaaaaacctc atcgccttca acaaacccta
      361 tggcgttatc tgccaatttt caccgcacga aaaacacaaa agcctcaaag actttatcaa
      421 tcttcccggc ttctaccccg ccggacggct cgacaccgac agcgaggggc tgctgctgct
      481 gaccgacgac ggcaggcttc aggcacaaat taccgacccc aaattcaaac accctaaaac
      541 ctactgggcg caactggagg gcgtacccga cgaaagccga ttggaaagcc taagaaaagg
      601 gatagactta ggcggtttcg ttacccgtcc ggcaagcatc cgcatcttga aacacggaga
      661 agcagattcg ttatgggagc gcatcccgcc gatacgcgtc cgcaaaaccg ttcccgattt
      721 ttggattgaa attaccattt ctgagggcaa aaaccgccaa gtcaggcgaa tgaccgccaa
      781 ggcgggctat ccctgcctgc gtctgatcag agtggcaagc ggcaggctga aactgtttga
      841 tttggattta aaacccgggg aatgggcata cgccccgttt aaaccataat cacgtttatc
      901 tcatcatttc cacaaaagtg ggaatccgga att
//
LOCUS       DAWKQJ010000252          906 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000252 DAWKQJ010000000
VERSION     DAWKQJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.252"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(61..840)
                     /locus_tag="WJL20_001994"
     CDS             complement(61..840)
                     /locus_tag="WJL20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ7684095.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          220 a          288 c          179 g          219 t
ORIGIN      
        1 ggtttaaatt tagttcacta taccgataca tccgaatatg ccagaccgtc taaacggtat
       61 tcatacctcc caatcctgca cgcgcttcaa atcgttttgc gccaaagtat ccgcgtggcg
      121 gttacggctc tgatattccc tgtctttcat gatgctgctt gccacataat tcaaatgtgc
      181 ctttgccgcc tccgaagcct cgcccggccg acggttcgat atcgcctcat acagcacgcg
      241 gtgctgcgcc atcagtttcg gacgcggatc ttcttcctga ttcagataaa taaggctgct
      301 gcgcgtctgc cggtacagca ttttcaacaa accgcccgac aaatggctga acaacaaatt
      361 gtgcgccgca tccgcaatcg tctgatggaa gctgacatcg gcttcgctct gatgttccag
      421 attgccgctt tcgcacgcct cctcaaactt ttcaagccaa aacccaatcc gcttcaaatc
      481 ggcatccgtc cggcgttctg ccgccaatgc cgccatacag ccctcgatgt ggcagctgaa
      541 atcaaaaaca tcctgttccc aattggaatg cttgcccaaa agctcctgcc aactttgcaa
      601 aaaatcctgc tgcggcttga ccgaaacata ataaccgtct ccctgcctcg cttccaaaac
      661 ctgacgggca accaaaacat tcaatgccga cctgaccgac gggcgcgaaa cgccgaactc
      721 ttccgccaaa acgcgttcgg gcggaatctt gcccccttcc gcgtaaaccc cttccacgat
      781 gcggccttcc aataccgaca atacctgatc gctgattttc tgaggtctta ccagtttcat
      841 cactcctcct ttataaagat tccctgcaga acccttccga aatatagtgg attaacaaaa
      901 atcagg
//
LOCUS       DAWKQJ010000253          888 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000253 DAWKQJ010000000
VERSION     DAWKQJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.253"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            220..>888
                     /locus_tag="WJL20_001995"
     CDS             220..>888
                     /locus_tag="WJL20_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7684096.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          253 a          196 c          203 g          236 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttgg aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcatagac
//
LOCUS       DAWKQJ010000254          844 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000254 DAWKQJ010000000
VERSION     DAWKQJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.254"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            652..>844
                     /gene="pilE"
                     /locus_tag="WJL20_001996"
     CDS             652..>844
                     /gene="pilE"
                     /locus_tag="WJL20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ7684097.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGEW"
BASE COUNT          283 a          231 c          144 g          186 t
ORIGIN      
        1 gggaatgacg aagctatcca tacggaaacc tgcaccgcgt cattcccacg aaagtgggaa
       61 tccaggacgc aaaatctcaa gaaaccgttt tcccgataag tttccgtacc gacagaccta
      121 gattcccgcc tgcgcgggaa tgacgaagcc atccgcacgg aaacctgccg cgatgattca
      181 actgccgttt gcacaggaac accacgcgcc gatttcaatc actttccaag aaaacggaaa
      241 tttttaaaaa ttaaatttaa aatttcccac ccaacccacc ctatgctacc gcgcaaattc
      301 aaaaatctca aattccgacc caatcaacac acccgatgcc ccatgccaat agagataccc
      361 cacgccaata aaaaagtgcc gaaaatcggc actaaaactg acaattttca acactgatga
      421 caaaaaacgg taatcccctg cttcctcaaa ccacacccaa acgaaagaaa atgtaaaata
      481 aaaacaacca tataaagata aacgcataaa ttttcacctc aaaacacaaa cttgatacca
      541 atcttgcttt ataatacaca ggtatcgcaa caaaataccg atggtcaaat acattgcata
      601 atgccgatgg cgtaagcttg aggcatttcc tttccaatta ggagtaattt tatgaacacc
      661 cttcaaaaag gttttaccct tatcgagctg atgattgtga ttgccatcgt cggcattttg
      721 gcggcagtcg cccttcctgc ttatcaagac tacacagccc gcgcgcaagt ttccgaagcc
      781 attcttttgg ctgaaggtca aaaatcagcc gtcaccgagt attacctgaa tcacggcgaa
      841 tggc
//
LOCUS       DAWKQJ010000255          831 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000255 DAWKQJ010000000
VERSION     DAWKQJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.255"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..498
                     /gene="mafB"
                     /locus_tag="WJL20_001997"
     CDS             <1..498
                     /gene="mafB"
                     /locus_tag="WJL20_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7684098.1"
                     /translation="AAVSGDFSAAYNTRITRKVTTETEGLNRIRQNQKNSNIHEKNYG
                     RDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTS
                     PKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPK
                     YNPVK"
     gene            513..743
                     /locus_tag="WJL20_001998"
     CDS             513..743
                     /locus_tag="WJL20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7684099.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          317 a          137 c          156 g          221 t
ORIGIN      
        1 gctgcggtta gcggggattt ttctgctgca tacaatacaa gaataactag aaaagttact
       61 acagaaacag aggggttaaa tagaatcaga cagaaccaga aaaatagtaa tatacatgag
      121 aaaaattatg gaagagataa tcctaatcat attaatgttt tatctggaaa ttctatacaa
      181 catatactgt atggagatga agcaggaggt gggcatcttt ttcctggcaa acctggtaag
      241 acaacattcc cccaacattg gtcagccagt aaaataactc atgaaattag tgatatcgtt
      301 acatccccaa aaacgcaatg gtatgcacag actggaacag gcggcaaata tattgctaaa
      361 ggaagaccag ccaggtgggt atcatatgaa acgagagatg gaattcgtat cagaacagtt
      421 tatgaacctg caacaggaaa agtggtaact gcattccccg atagaacctc taatcccaaa
      481 tataaccctg taaaataatg agtatttgaa agatgaaaac attagacgaa cgaataaaaa
      541 atttaggaaa atctcttgaa gatagaattg acgctaatct aattgatgcc gcactagagt
      601 acattacttt ttcagaacgt ttgctagctt ttgaaacgct atgtgattat atagaagatt
      661 tcaatatcca gcttacggaa aaagaatccc aagaaatctc ttttattaat aaggaatttg
      721 gaattgaaag tacatcagat taataaatat taggtcggtt taaaaaccca acaatcaaaa
      781 agtcgtctga aactgtgttt aatttacaga cgacctttcc ttcatttgaa g
//
LOCUS       DAWKQJ010000256          813 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000256 DAWKQJ010000000
VERSION     DAWKQJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.256"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..420
                     /gene="mafB"
                     /locus_tag="WJL20_001999"
     CDS             <1..420
                     /gene="mafB"
                     /locus_tag="WJL20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7684100.1"
                     /translation="AAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKI
                     HDGAQGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYP
                     IGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            423..740
                     /locus_tag="WJL20_002000"
     CDS             423..740
                     /locus_tag="WJL20_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684101.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEERRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
BASE COUNT          283 a          123 c          166 g          241 t
ORIGIN      
        1 gctgcggtta gtggggattt ctcagactcc tacaagcata acactgcttc aagattatct
       61 cagtctgtag atggagaaat gtttcaaacc cgcaatgttg attttaaagc aaaatctatt
      121 gggactaaaa ttcatgatgg agctcaaggg aaacatattt caggacatag aaactacatt
      181 gaaggtaaga gtactttaaa tcaaaacatt aatcctcaag aattgttgaa cggaatacat
      241 tcaggtgctt atccagttat ttctaaagga gcaagaggaa atcctgttgt tgattttggg
      301 tatcctatag gcagcgatgg gaaatcagga ttaagtacca attttggtac gattcattca
      361 ggtaaaaatg gagttcacat tgttccggct aaccctaaaa ccattaaaaa ggtgcaatag
      421 ttatgaatat attaccaagc tggctgcgag tcggtatgaa tatagcaatg ctgggcatga
      481 tacactcaga tatcaggtta attaccgtag attacgagga aagaagaagg tttttaaaaa
      541 tcaaaaatta tttatcaaga gaagccatca cagaagacca tgaagatatg gaatatttga
      601 ttacagagtt atggtctatg tgtggagaat attttgatga agctgacttt gaatgtattt
      661 attctaatca ttcttctatg gagttaaacc aaataaatgg tgcagtattc aggagaaagg
      721 aattaatttc gcaagcgtag gttaaaaaac caacaatcac aatgtcttct gaaaccgtgt
      781 ttaattttca gacggcattt ccttcatttg aaa
//
LOCUS       DAWKQJ010000257          807 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000257 DAWKQJ010000000
VERSION     DAWKQJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.257"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..807
                     /locus_tag="WJL20_002001"
                     /pseudo
     CDS             1..807
                     /locus_tag="WJL20_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT          232 a          210 c          191 g          174 t
ORIGIN      
        1 gtgcattgtt cgaccaccgc aatcaaggca tccgcaccgc caccggttgg gttgctgccg
       61 atgacggtct gcttgtgcgc gatttgaacg gcaacggcat catcgacaac ggcgcggaac
      121 tcttcggcga caacaccaaa ctggcagacg gttcttttgc caaacacggc tacgcggctt
      181 tggccgaatt ggattctcaa acggcgacaa catcatcaac gcggcagacg ccgcattcca
      241 aaccctgcgt gtatggcagg atctcaatca ggacggcatt tcccaagcta atgaattgcg
      301 tacccttgaa gaattgggta tccaatcttt ggatctcgcc tataaagatg taaataaaaa
      361 tctcggtaac ggtaacactt tggctcagca aggcagctac accaaaacaa acggtacaac
      421 cgcaaaaatg ggggatttac ttttagcagc cgacaatctg cacagccgct tcaaagacaa
      481 agtggaactc actgccgaac aggcaaaagc cgccaatctt gcgggcattg gtcgtctgcg
      541 cgatttgcgc gaagctgccg cattgtccgg cgatttggcc aatatgctga aagcttattc
      601 tgccgccgaa actaaagaag cacagttggc attgttagat aatttgattc acaaatgggc
      661 ggaaaccgat tcgaactggg gcaaaaaatc gccaatgcga ctttcaaccg attggacgca
      721 aacggctaat gaaggtattg cactgacacc atcccaagta gcacaactaa aaaagaacgc
      781 tttagtttcc ctttctgata aagctaa
//
LOCUS       DAWKQJ010000258          805 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000258 DAWKQJ010000000
VERSION     DAWKQJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.258"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..743)
                     /locus_tag="WJL20_002002"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WJL20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7684102.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWKQJ010000259          798 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000259 DAWKQJ010000000
VERSION     DAWKQJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.259"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            337..636
                     /locus_tag="WJL20_002003"
     CDS             337..636
                     /locus_tag="WJL20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7684103.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            615..798
                     /locus_tag="WJL20_002004"
                     /pseudo
     CDS             615..798
                     /locus_tag="WJL20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          218 a          218 c          174 g          188 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacagga aaaaactaca ccaaactaca
      121 atttttgttt atgcaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 actctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttatttgc cgtgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgcattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggaaaat ccacacgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc accatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaac
//
LOCUS       DAWKQJ010000260          768 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000260 DAWKQJ010000000
VERSION     DAWKQJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.260"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(171..>768)
                     /locus_tag="WJL20_002005"
     CDS             complement(171..>768)
                     /locus_tag="WJL20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7684104.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          170 a          221 c          201 g          176 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acccaaacac agtcaaacaa
       61 atttatatgc cccatccctt ccgaataatt tgaaaacaca gccgccaaaa acaaaaatgc
      121 cgtctgaaaa cctttcagac ggcatttcca acttgatttc aggcagaaag tcagaacgcg
      181 atatagctgt tcggattaac cggtttgccg ttttgccgca cctcgaaatg aagctgcgtt
      241 ctggaagcgt cggtattgcc catcaaagcg acctgctgcc cgcgtttgac ctgctggcct
      301 tcgccgacca gcaatttttg gttgtgcccg tatgcggtca ggaaggaaga attatgctgg
      361 atgatgacca aattgccgta tcccctcaaa ccggaacctg cataaaccac tttgccgtca
      421 gccgccgcca aaacgggctg tcccgcattt cctgcaatat cgacaccctt attgttgccg
      481 ccgaaatcgg caaccacttt accttgcgtc ggacgctgcc aaacaatgcc gccgaccgaa
      541 cgcgtgccgg aaggcgaagc ggcaggagat tgcggggcgg gcgcgggaac cgctttattt
      601 tccgcagcgg gcgcggcagg ttgcggcgcg gactgcacag gcggttgcgc ggcgggtttc
      661 acaggggttt gcacggcagc cggtacggcg ggcctgcttt ctacggctga ggttttcggt
      721 gcggcatatc ctgccggttt gactttaaca atctgaccga tgctcaac
//
LOCUS       DAWKQJ010000261          757 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000261 DAWKQJ010000000
VERSION     DAWKQJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.261"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..>757
                     /gene="rpe"
                     /locus_tag="WJL20_002006"
     CDS             44..>757
                     /gene="rpe"
                     /locus_tag="WJL20_002006"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ7684105.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEM"
BASE COUNT          190 a          174 c          214 g          179 t
ORIGIN      
        1 atttttgtta atccactata aaaggatatt gaaggaaaaa aacatgacta cttaccgtat
       61 cgcacccagc attttatcgg cagattttgc acggcttggc gaagaggtgg aaagcgtcat
      121 tgcggcaggt gcagatctga tccattttga cgtgatggac aaccattatg tgccgaacct
      181 gaccttcggg cctatggttt gcgcggcgtt gaagccttat gcaagcgtgc cgatcgatgt
      241 gcatctgatg gtcgaacccg tggatgacct gattcagtcg tttgccaaag caggagcatc
      301 aatcatcacg ttccatcccg aagcgagccg ccatattgac cgcagcttga gcctgattcg
      361 ggacatgggc tgtcaggcgg ggctggtgtt gaatccggca acgcccgtat atctgttgga
      421 aaacgtattg gacaggctgg atatggtttt gctgatgtcg gtaaaccccg ggttcggcgg
      481 tcagagcttc attccgtata ctttggaaaa aatcagacag gttcgtgcca tgcttgaccg
      541 ctatgaagag aaaagcggca gacgtattgc cattgaagtg gacggcggca tcaaaaccga
      601 caatatcgcc gccgccgccc aagccggcgc ggataccttt gttgccggtt ctgcgatttt
      661 cggcaaacca gactataagg cggtgattga tgcgatgagg gcggaactgg aaaaaagcgg
      721 caggctgaac cttgcgccaa tgattgaaat agaaatg
//
LOCUS       DAWKQJ010000262          748 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000262 DAWKQJ010000000
VERSION     DAWKQJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.262"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>748
                     /locus_tag="WJL20_002007"
     CDS             <1..>748
                     /locus_tag="WJL20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7684106.1"
                     /translation="QSYGEQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAK
                     PDAVNLSASQGIDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQ
                     KRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATI
                     RAGVGEQARADAKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPV
                     APVLSAPGGYIVDIPQGNLKTQIETLTKQPE"
BASE COUNT          242 a          223 c          166 g          117 t
ORIGIN      
        1 tcaatcttac ggtgaacaat attactatgc gagacagagc gaactctata cctttgaacg
       61 ccgcagctac aaaaccggca aatggtacaa ccgcaaacac attaccgaag tcaaagaaca
      121 caaaaacgcc aagcccgacg cagtaaacct cagcgcatcc caaggcatcg acatcaaatc
      181 tggtggcagc atcgacgcct acgccaccgc attcgatgcc cccaaaggca gcattaacat
      241 cgaagccggg cggaaattga cactctatgc cgtagaagag ctcaactacg acaaactaga
      301 cagccaaaaa aggcgcagat ttctcggcat cagctacagc aaagcacacg acaccaccac
      361 ccaagtcatg aaaaccgcgc tgccctcaag ggtagttgca gaatctgcca atctgcaatc
      421 aggttgggat accaaactgc aaggcacaca gtttgaaacc acactgggtg gcgcaaccat
      481 acgcgcaggc gtaggtgagc aggcacgggc agatgccaag attatcctcg aagggatcaa
      541 aagcagcatc cacacagaaa ccgtgagcag cagcaaatct actctatggc aaaaacaggc
      601 aggacggggc agtaacatcg aaaccttgca attgccgagt ttcaccggtc ccgttgcgcc
      661 cgtactgtcc gcacccggcg gttacattgt cgatattccc caaggcaatc tgaaaaccca
      721 aatcgaaacc ctcaccaaac agcccgag
//
LOCUS       DAWKQJ010000263          733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000263 DAWKQJ010000000
VERSION     DAWKQJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.263"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>733
                     /locus_tag="WJL20_002008"
     CDS             <1..>733
                     /locus_tag="WJL20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7684107.1"
                     /translation="AQNSGLIPSLNIGLDELAKSGMGEYIARTDADDIAAPDWIEKIV
                     GEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHGKIWKKPTRPEDIADFFPFGNPIH
                     NNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSS
                     KYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLPEEDFER
                     ARRFLYQCFKRTDTLPAGAWLDFAAD"
BASE COUNT          200 a          187 c          195 g          151 t
ORIGIN      
        1 gctcaaaatt ccggcctgat tccctcttta aacatcgggc tggacgaatt ggcaaagtca
       61 ggaatggggg aatatattgc acgcaccgat gccgacgata ttgccgcccc cgactggatt
      121 gagaaaatcg tgggcgagat ggaaaaagac cgcagcatca tcgcgatggg cgcgtggctg
      181 gaagttttgt cggaagaaaa ggacggcaac cggctggcgc ggcatcacag gcacggcaaa
      241 atttggaaaa agccgacccg acccgaagat attgccgact ttttcccttt cggcaacccc
      301 atacacaaca acacgatgat tatgaggcgc agcgtcattg acggcggttt gcgttacaac
      361 accgagcggg attgggcgga agattaccaa ttttggtacg atgtcagcaa attgggcagg
      421 ctggcttatt atcccgaagc cttggtcaaa taccgccttc acgccaatca ggtttcatcc
      481 aaatacagca tccgccaaca cgaaatcgcg caaggcatcc aaaaaaccgc cagaaacgat
      541 tttttgcagt ctatgggttt taaaacccgg ttcgacagcc ttgaataccg ccaaataaaa
      601 gcagtagcgt atgaattgct ggagaaacat ttgccggaag aagattttga acgcgcccgc
      661 cggtttttgt accaatgctt caaacggacg gacacgctgc ccgccggcgc gtggctggat
      721 tttgcggcag acg
//
LOCUS       DAWKQJ010000264          732 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000264 DAWKQJ010000000
VERSION     DAWKQJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.264"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>732
                     /locus_tag="WJL20_002009"
     CDS             <1..>732
                     /locus_tag="WJL20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7684108.1"
                     /translation="ALWFGPVQNGKAQMYSASVSTYPDSSSSRIYLQNLKRKTDPNKP
                     GRYSLADLTKSDIESRQPGFTGRQTVIRLDGGVRHIQLDRSNEATGLNGNDGKNDTFG
                     IVSEGSFMPDASEWKKVLLPWTVRASNDDGRFNTFNKEENNGKPKYSQKYRSRDNNNR
                     DLGDIVNSPIVAVGGYLATSANDGMVHIFKQSGGGDDRNYSLKLSYIPGTMPRKDIES
                     KESTLAKELRAFAEKGYVGDRYGVDG"
BASE COUNT          207 a          191 c          192 g          142 t
ORIGIN      
        1 cgcgctgtgg ttcggacctg tgcaaaatgg taaggcgcag atgtattccg cttcggtttc
       61 tacctacccc gacagttcga gcagccgcat ctaccttcaa aatctgaaaa gaaaaaccga
      121 ccccaacaaa cccggtcgct attctctcgc agacttgact aagtcggata ttgaaagtcg
      181 acagccgggt ttcacagggc ggcaaaccgt catccgattg gatggcggcg tacggcatat
      241 ccaactggat agaagcaatg aggccaccgg tttaaatgga aatgacggca aaaacgacac
      301 tttcggcatt gttagtgaag ggagcttcat gcctgatgcc agcgagtgga aaaaagtatt
      361 gctgccttgg acggttcgtg cttccaatga tgacggtcga tttaacacat tcaacaaaga
      421 agaaaacaac ggcaagccaa aatacagtca aaaataccgc agccgcgaca acaacaatcg
      481 cgatttgggc gacatcgtca acagccccat cgtggcggtc ggcgggtatt tggctacttc
      541 cgccaacgac gggatggtgc atatcttcaa acaaagcggc ggcggggatg accgcaacta
      601 tagtctgaag ctcagctaca tccccggcac gatgccgcgc aaagatattg aaagcaaaga
      661 atccaccctt gccaaagagc tgcgcgcctt tgccgaaaaa ggctatgtgg gcgaccgcta
      721 cggcgtggac gg
//
LOCUS       DAWKQJ010000265          720 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000265 DAWKQJ010000000
VERSION     DAWKQJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.265"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            180..509
                     /locus_tag="WJL20_002010"
     CDS             180..509
                     /locus_tag="WJL20_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7684109.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..720
                     /locus_tag="WJL20_002011"
                     /pseudo
     CDS             538..720
                     /locus_tag="WJL20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          220 a          193 c          183 g          124 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaggcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggtgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggaaggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gcgggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcccggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaccga caaagccacc
      661 ggcgccaaag ccgacgacgt caaagccgcc accgccaacg gcaagaccgc cgacaacatc
//
LOCUS       DAWKQJ010000266          706 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000266 DAWKQJ010000000
VERSION     DAWKQJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.266"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            28..705
                     /locus_tag="WJL20_002012"
     CDS             28..705
                     /locus_tag="WJL20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ7684110.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          218 a          137 c          156 g          195 t
ORIGIN      
        1 catcaaaaaa cctgataagc tcctaatatg aaactaaaaa ataagtatca aaagttcagc
       61 aaaatttcag agcaaaagtt tagagaaata atccgctgtt tcgctcttga tttgaccgct
      121 tccgatactg ctaaaatgac cggcattagt gtacgcggta tcaatcctat tttcctgaaa
      181 atcagacaca ggattgctgc tctgtgcgaa caaagctcac cactgtccgg tgtggtcgaa
      241 ttagatgaat cttactttgg tgcgcgacgt atcaggggta agcgcggtcg cggagcgtca
      301 ggtaaaacca tcgtatttgg catactgaaa cgcaatggcg tggtctatac ggaaatcgtt
      361 cccgacgctt cgaaagcctc actaatcaag gtcatacgtg gtcacatatc tgctgacagt
      421 gaaatcaata ctgacggctg gaaagcatat gacggtcttg tcgatatggg ctatgagaag
      481 cattaccgtg tccatcatgg ttccaatgag tttgctcgag gaaagcaaca tatcaatggt
      541 attgaatctt tttggagtta tactaagggg cgtttggcta aatttcatgg tatttccaaa
      601 gagatgtttt atctgcatct caaagaaacg gagtttaggt ttaaccaccg gcatgaagat
      661 ttaggtaaaa tattaatgaa gatgcttcga aataacccaa tttaat
//
LOCUS       DAWKQJ010000267          693 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.267, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000267 DAWKQJ010000000
VERSION     DAWKQJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.267"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(91..642)
                     /locus_tag="WJL20_002013"
     CDS             complement(91..642)
                     /locus_tag="WJL20_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684111.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNILQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
BASE COUNT          191 a          275 c          133 g           94 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc ccaagctgcg gctgccaaac acatcggctg
       61 cttctcgacg cacccgctga aaaatgcgcc ctatccgccc gccttgaccg caccccctgc
      121 ctttgccgcc ctgcccgatg ccgtctgaag cctgaaccgc cccaccagcc cgatgacgca
      181 cgacaccgcc ggaaagggca tacacaaagc cgtcagcaca taacgcgcat tccccgtatt
      241 gaccacgtcc gcgcccttca gcagcagcgg cagcgcgttc gcccactccc acaccgacgg
      301 cgcactgaac gccaacaccg ccaccagccc caacatccgg aacaccccga tttccaacac
      361 ctgccgccgc cgcaccgaac ggttgagcag aaacacaacc gccaacccga aacagaccac
      421 cgccgccata ccgccgttgg cgacaatctg caaactgttg aatgcaatat cgggcgtaac
      481 cgccaacacg ccttccgaat ccggcacggc acgctgcaaa tccaaaccct gcaagatatt
      541 caacacaaaa ccgtagatca aatcaaaaaa caccacgcct atcggcaaag cactcaaaac
      601 ccgccacatc ttcctgtccg accaaccgtt caaaactttc attttacccg accacgcaag
      661 ccgccgaaca aaaaataagg ggctgtccta gat
//
LOCUS       DAWKQJ010000268          686 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.268, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000268 DAWKQJ010000000
VERSION     DAWKQJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.268"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..>686)
                     /locus_tag="WJL20_002014"
     CDS             complement(<1..>686)
                     /locus_tag="WJL20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7684112.1"
                     /translation="AMAELVPGLSAHPYLSSVEKACFMSHVVLWKQALDEGVPYVAVF
                     EDDVLLGEGAEKFLDEDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPL
                     LESEHWGTAGYIISRKAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPA
                     LCAQELHYAKFHDQNSALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISRERE
                     KRRQRREQLIG"
BASE COUNT          150 a          198 c          186 g          152 t
ORIGIN      
        1 ccgattaact gttcgcgcct ttgccgacgt ttttcccttt ccctgctgat tttggtcaag
       61 gcgcggatca ggcggcgttt gaatgtgttg gcgggggaat cgcgccattg ctgtttgcgg
      121 ttcaggaggc ggtcgtgttc gatcaggctg cccaatgcgc tgttttggtc gtgaaacttg
      181 gcataatgca gctcttgggc gcacaaggcg ggattgagct ggcaaaccgg cactccttcc
      241 ctgtcgaaaa aatcgctgaa catcatcaga tcgacggggt gcagcccttc gggcggcagg
      301 gcggcaaacc tgtccaggaa aaaccgcatc gcttttcggg aaatgatata gcccgccgtc
      361 ccccagtgtt cgctttccaa cagcggaaag gcacgcccgc agtagtccgc cacaccggag
      421 ggcgaggtca ggacgtgcat aaacatcgtt tccaagcgga cgataaaggc ggaatccttg
      481 tcaaaacgct cttgcagcca agcgtcttcg tcaaggaatt tctccgcccc ttcgccgagt
      541 aagacatcat cctcaaatac ggcaacatac ggtacgcctt cgtccaacgc ctgcttccac
      601 aatacgacgt ggctcataaa gcaggctttt tccacactac tcaaataagg atgcgccgac
      661 aagccgggta cgagttccgc cattgc
//
LOCUS       DAWKQJ010000269          653 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.269, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000269 DAWKQJ010000000
VERSION     DAWKQJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.269"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WJL20_002015"
     CDS             complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WJL20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ7684113.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          131 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attccccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaacac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaacctgc cgccatcaaa aacaacgtcc agccgccgcc caaccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaggactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cac
//
LOCUS       DAWKQJ010000270          641 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.270, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000270 DAWKQJ010000000
VERSION     DAWKQJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.270"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(92..376)
                     /locus_tag="WJL20_002016"
     CDS             complement(92..376)
                     /locus_tag="WJL20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7684114.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          184 a          162 c          108 g          187 t
ORIGIN      
        1 cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaaaaccga aaccgaacaa
       61 gccggattcc cgcctgcgcg ggaatgacgg gctaaataat atcaaaccat aaatcctgcc
      121 aagaaacatt attttcttca atcagttgca atttccaagc cctgttccat ttcttcaact
      181 gtttttcccg agtaattgcg ctctccatcg taggatgcag ttcataccaa accagcatag
      241 taacgttgta ccgtgatgta aatccctcaa tcaaatgttc cctatgttgg taaatacgtt
      301 gcaccaaatc agatgtaacg ccaatgtata acgtgccatt acgttggctt gctaaaatat
      361 aaaccgcagg ctgcatataa tacccttttg aattatttaa atttatattc ccgcgaacac
      421 catcccgtga ttactttaac ccttcgttat tcccatagct ttccatcatt cccgcaactc
      481 ttcgtcattc ccacgaaagt gggaatctag aatctctaaa gcttcagcta acctttgaat
      541 attactgttg ttctaaggtc tagattcccg cctgcgcggg aataacgggt cttttataaa
      601 ctttgaatat tgccgttatc ccaaggtcta gattcccgcc t
//
LOCUS       DAWKQJ010000271          626 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.271, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000271 DAWKQJ010000000
VERSION     DAWKQJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.271"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(81..260)
                     /locus_tag="WJL20_002017"
     CDS             complement(81..260)
                     /locus_tag="WJL20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684115.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
     gene            447..>626
                     /locus_tag="WJL20_002018"
     CDS             447..>626
                     /locus_tag="WJL20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7684116.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSM"
BASE COUNT          180 a          180 c          114 g          152 t
ORIGIN      
        1 tgtcctgatt tttgttaatc cactatatcc gctccaaagc aaatgccgtc tgaaaacctt
       61 tcagacggca tttgttgtct ttattgccgt ttttcgtccg tatccggatt tttgtttttc
      121 agcttcgcac ccaagcccaa acgcctttca taatccgatt gcggagtatc gtcttcctgc
      181 ataccgaacg cgccggcatt gacccacagc gacagcagcg cgacgacaaa ggcgcaaaag
      241 ccaatcacat accaaaacat tgcccctccc gatttgttaa aatcatatca aatacagtgc
      301 cgaatttatc acaaacgcac gggcaaatat agtgaattaa atttaaataa ggacaaggcg
      361 gcgagccgaa gacagtacaa atagagacct ttgcaaaatt ccccaaaatc ccctaaattc
      421 ccaccaagac atttaggaga tttctcatga gcaccttctt ccaacaaacc gcccaagcca
      481 tgattgctaa acacatcgac cgtttcccgc tattgaagtt ggaccaggtg attgattggc
      541 agccgatcga acagtacctg aaccgtcaaa aaacccgtta ccttagagac caccgcggcc
      601 gtcccgccta ccctctgctg tccatg
//
LOCUS       DAWKQJ010000272          600 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.272, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000272 DAWKQJ010000000
VERSION     DAWKQJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.272"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..151
                     /locus_tag="WJL20_002019"
     CDS             <1..151
                     /locus_tag="WJL20_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684117.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            173..466
                     /locus_tag="WJL20_002020"
     CDS             173..466
                     /locus_tag="WJL20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684118.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          159 a          151 c          160 g          130 t
ORIGIN      
        1 gtccattcgg cagggaaagc ctgaagaaga attttcccgc aagatggccg gctgcctaac
       61 cgacttggaa aactgtttgg aaaaatacct cgaacagttc gggccggtat cggaaagtat
      121 cgaagcctgt accgtcaaac tgcaggagta accgtcgttc ttcaacaggc tgatgaaaac
      181 caacgacaag ctcaataggc agattgacgt gttgcagaaa cagtcggcag ccatccacaa
      241 cgaagcctat atcgaaatga acacgctgct ttaccgccac cgcgaagtgg tttccgtcca
      301 caaccgcaag gcggattatg cggaaaaggg caaagagcgg attgcgctgt tcccgcgcgg
      361 cttgaacggc atcaccaagc tgcctgccgc cgtcctgttg cccgagcgtc ccgattgttt
      421 tgatatgaag gcagtttggc aaatgtttaa tagaataccg aggtaggcgt atcggctgcc
      481 ggataaaaat gccgtctgaa acggaagcgt tcagacggca ttgttgcagt ggaacaaatc
      541 aaaatgcacc ccgtcattcc tgtctgtacg gtgatagcgg cagtgttttc ctgcttcgtt
//
LOCUS       DAWKQJ010000273          599 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.273, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000273 DAWKQJ010000000
VERSION     DAWKQJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.273"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..497)
                     /locus_tag="WJL20_002021"
                     /pseudo
     CDS             complement(1..497)
                     /locus_tag="WJL20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          180 a          126 c          121 g          172 t
ORIGIN      
        1 ggttccaaaa gttctcaatt ccattaatat ggttttgtcg ttcggcaaaa tgtgtgctgt
       61 gattgatacg aaaacgaagt ttcagcgaag ctaaaatggc taaattcgcg cacatctaat
      121 acatcatagc tacgataaca atccgtataa aaaatgctgt caggtttcac ttgttcacgg
      181 ataataggaa ataaagtagc ggtttgagta ttcggtactg taaccgtata aaccttacca
      241 tttcgcttca aaagaccgaa tacggcgact ttaccggcag caccgcgacc acgtttgcct
      301 ttgcgttgtc cgccaaaata actttcatct gcttctactt cgccatcaaa catttctaaa
      361 tgtgggctgt tttgataaat aagtaatcgt aaacgatgaa aataataggc tgcggtattt
      421 ttattaacgc ctactaactc tgctgccttt cttgcagtta cgcctgcgac aaacagttca
      481 atgagtttat agtggattaa atttaaatca ggacaaggcg acgaagccgc aggcagtaca
      541 aatagcacgg aaccgattca cttggtgctt gagcaccaag tgaatcggtt ccgtgctat
//
LOCUS       DAWKQJ010000274          598 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.274, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000274 DAWKQJ010000000
VERSION     DAWKQJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.274"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..558
                     /locus_tag="WJL20_002022"
     CDS             <1..558
                     /locus_tag="WJL20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7684119.1"
                     /translation="DTERFFPQPGETADLRAKYGFADHETVFLFPSTGHTRKGLELLA
                     DFFEHTGLPVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVG
                     VESVLCGTRVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSD
                     PMRALNYNPSLSHHIDRLTDMLASV"
BASE COUNT          132 a          206 c          139 g          121 t
ORIGIN      
        1 gatacggaac gcttctttcc acaacccgga gaaactgccg acctgcgcgc caaatacggc
       61 tttgccgacc atgaaaccgt tttcctgttc ccatcgaccg gccacacgcg caaaggtctg
      121 gaactgcttg ccgacttttt cgaacatacc ggcctgcccg tcaagcttgc cgttgtcggc
      181 tccccgctcc cccgccctat gaaaaacgtc gtcggactgg gcttctgcac cgatatgccc
      241 gaactctacc gcgccgccga ctttaccatt atggcatccc tgtacgaacc cttcgggctg
      301 gtcggtgtcg aatccgtcct atgcggcaca cgcgtcgtcc tctccgaaaa catggcatgt
      361 acagaggtca tgaacgaaga agccggattc tttttctcac gccaaaaccc ggaaaccctg
      421 gcgcaagccg ttgcccaagc cgtcagcctt aaaaaacagg gcggacaccg cctgtccgac
      481 ccgatgcgtg cactgaacta caacccgtcc ctgtcgcacc acatcgaccg actgaccgac
      541 atgctggcat ccgtataaca aaatgccgtc tgaacgagtt ttcagacggc attgctgc
//
LOCUS       DAWKQJ010000275          596 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.275, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000275 DAWKQJ010000000
VERSION     DAWKQJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.275"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..107)
                     /locus_tag="WJL20_002023"
                     /pseudo
     CDS             complement(1..107)
                     /locus_tag="WJL20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            397..>596
                     /locus_tag="WJL20_002024"
     CDS             397..>596
                     /locus_tag="WJL20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684120.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIV"
BASE COUNT          162 a          103 c          182 g          149 t
ORIGIN      
        1 gcaaaaccgg ctctgcccgc agctaaaaaa taggggaacc tgtaaaggaa ggggcatcgg
       61 ccgccgtcgg tgcttttttg tttggaaggg aaatggctgg aaaaagacct gcgctatatc
      121 ggggaatgaa tcgacgcaaa gaaaatgccg tctgaacatg cgttcgggcg gcgttttgtt
      181 ggggggtatc ggagcggaac gtctgaaaaa gggtttcagg cggtctttgg gcgtgtggta
      241 acagtcgaaa acgtgataag gctacctgaa aagtttggga gattttcagg tagtctttgg
      301 tattgggcgc aacagacgca ggtacagatt aggcggtgtg ccgtaatcgt acgaatgccg
      361 attcaaccta agcagacatc agtatttagg aagtggatgt ttgatggagc aaaggttgta
      421 cgaagggtgg aaggcaacct gtgggtgttt ggtatggtcg cgcttgaaaa aacgtgtttt
      481 aagggacaaa tgccgtctga aaatcggttt cagacggcat tttctgttta tttaaagcaa
      541 acaggaaaag gcagcaatat tctgcagtct tcctattcac acaagcgttt tatagt
//
LOCUS       DAWKQJ010000276          591 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.276, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000276 DAWKQJ010000000
VERSION     DAWKQJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.276"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..306
                     /locus_tag="WJL20_002025"
     CDS             <1..306
                     /locus_tag="WJL20_002025"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ7684121.1"
                     /translation="EQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVK
                     SDRQKPAQYIGQHQDAYLHEIAKHFDCTAATVCYALKQMGITRKKRPPLTKNKTRPK"
     gene            210..>591
                     /locus_tag="WJL20_002026"
     CDS             210..>591
                     /locus_tag="WJL20_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684122.1"
                     /translation="MYGSHRLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQSAEFSD
                     YQRVYLDETGFDRYLFRPYTRSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSV"
BASE COUNT          192 a          146 c          123 g          130 t
ORIGIN      
        1 gaacaatgca aaaacatcag ccaaaccgca gcaacgttta acttgtcaag aaacacgctt
       61 tacctgtgga ttcgccttaa aaaacaaaca ggcagcctaa aacatcaagt taccggtcta
      121 aatgccgtca aatcggatag gcaaaaaccg gctcaatata ttgggcaaca ccaggatgcc
      181 tatctgcatg aaatcgccaa acattttgat tgtacggcag ccaccgtttg ctatgcactc
      241 aaacagatgg ggataacgcg caaaaaaaga ccaccactta caaagaacaa gacccggcca
      301 aagtaacgca ttatttgaca cagtcggccg aattttccga ctaccaacgt gtttatttgg
      361 atgaaacagg atttgaccgc tacctgttcc gtccctatac ccgcagcctg aaagggcaaa
      421 tagtgaaagc gcagataagt ggaaaaagat accaacgctt atctttggtg tccgcacaag
      481 tcggcaaccg gctgattgct ccgatggttt atcaaaatac gatgaccgga gtcttttttg
      541 aagcgtggtt tcagcaatgc ctactgcccg cattgactca aaaatcggtg a
//
LOCUS       DAWKQJ010000277          587 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.277, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000277 DAWKQJ010000000
VERSION     DAWKQJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.277"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            2..196
                     /locus_tag="WJL20_002027"
     CDS             2..196
                     /locus_tag="WJL20_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684123.1"
                     /translation="MSHSKLWANVACAAGTVKFVMLPDPSAEIWAVYLGIVGGYAVAR
                     SLVSVKRQEVENESRETAGE"
     gene            168..572
                     /locus_tag="WJL20_002028"
     CDS             168..572
                     /locus_tag="WJL20_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7684124.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTERDL
                     CKIPFPPDSRNPNTGFRLFSPQIPPNSTQIPP"
BASE COUNT          143 a          156 c          166 g          122 t
ORIGIN      
        1 tgtgtcgcac tcgaaactgt gggcaaacgt tgcctgcgcg gcggggacgg ttaagtttgt
       61 gatgctgccc gacccgtcgg cggagatttg ggcggtgtat ttgggcattg tcggcggcta
      121 tgcggtggcg cgttcgttgg tcagcgtcaa acgtcaggag gtcgagaatg aatctcgtga
      181 aactgctggc gaataactgg caaccaattg ccatcatcgc gcttgtcggc acgctcttgg
      241 cggtgtcgca ccatcaaggc tacaagtcgg cttttgcgaa gcagcaggcg gtcattgaca
      301 agatggaaaa agacaaagcg caagccctgc tgttgtcggc tcaaaactat gcacgcgagc
      361 tggaacaggc gcgtgcggaa gctaaaaaat atgaagtcaa ggcgcacgcc gtcggcatgg
      421 ctttggcgaa aaaacaggcg gaagtcagcc gtctgaaaac ggaaagagac ctttgcaaaa
      481 ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcggctgttt tcgccccaaa
      541 taccgcctaa ttctacccaa ataccccctt aatcctccac ggatacc
//
LOCUS       DAWKQJ010000278          572 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.278, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000278 DAWKQJ010000000
VERSION     DAWKQJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.278"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            212..571
                     /locus_tag="WJL20_002029"
     CDS             212..571
                     /locus_tag="WJL20_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7684125.1"
                     /translation="MTGFNDAAGVASASDIKGKYVEKVEVAKGVVTAEMKSTGVNNEI
                     KGRKLSLWAKRQDGSVKWFCGQPVTRDANNTADDAVKADTGTNGKIDTKHLPSTCRDA
                     SSVVCIETPPTAFYKNT"
BASE COUNT          165 a          159 c          132 g          116 t
ORIGIN      
        1 gcttcatctg ccaaccgtac aaaaacaccc ggtttcgctt tcaaatactt ccaaaaaatc
       61 agccgttacc gagtattgcc cgaatcacgg caaatgacca aaaaacttcg tcattcccgc
      121 gcaggcggga atctaggttt gtcggtgcgg aaacttatcg ggtaaaaagg tttcttgaga
      181 ttttgcgttc tggattccca ctttcgtggg aatgacggga tttaatgatg ccgccggcgt
      241 ggcatccgcc tccgacatca aaggcaaata tgttgagaaa gttgaagtcg caaaaggcgt
      301 cgttaccgcc gaaatgaaat caaccggcgt aaacaacgaa atcaaaggca gaaaactctc
      361 cctgtgggcc aagcgtcaag acggttcggt aaaatggttc tgcggacagc cggttacgcg
      421 cgacgccaac aacaccgccg acgacgccgt caaagccgac accggcacca acggcaagat
      481 cgacaccaag cacctgccgt caacctgccg cgacgcttca tctgtcgttt gcatagaaac
      541 accacctacg gctttctata aaaataccta aa
//
LOCUS       DAWKQJ010000279          549 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.279, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000279 DAWKQJ010000000
VERSION     DAWKQJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.279"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(16..507)
                     /locus_tag="WJL20_002030"
     CDS             complement(16..507)
                     /locus_tag="WJL20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7684126.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          161 a          138 c          115 g          135 t
ORIGIN      
        1 gaatccgaca ttacttcaat cgtatccaat agaaaagtcc gcattgccgc caccccaatt
       61 atgcggataa ataccctgtt tgacataacg gtgaaacgta gaaaaccccc aatcggaaat
      121 ttgtcctaca tagccatgtt tgaccggatt gaaatgcaga taatcaaaat gccaggcaaa
      181 atcggcctca tcgcggatag tatattccca aaagcgtttt tgccaaagcc tgagattgcc
      241 gccgattaaa tattggctgt gccgcttgat ttgccgccag cgttccgaat aagcagaatc
      301 attgtccggc agccgccata tggtatgaag atggtcgggc atcaacaccc atgccaaaat
      361 ttcaaacgga taccgttcgc gcaccgccat taccgcctgc cgcaaagcca aacgcaccgc
      421 atcatcggtc aaaatcttct gccgtttatt ggttacaacc gtaaaaaagt aagtgccgcc
      481 attgcggtaa aaacgacggt atttcatagt attatgctcg gaatgatttt gtaggtcgga
      541 ttcttgaat
//
LOCUS       DAWKQJ010000280          524 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.280, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000280 DAWKQJ010000000
VERSION     DAWKQJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.280"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          156 a          150 c          112 g          106 t
ORIGIN      
        1 atccactata tccgtttgcg cccgccaagc cgtgttgcac agatgcgaca cggctttttt
       61 ctttctgttg tcagaatgta aattttcgtc aaaaaacatc aaaaatcatc cccccccgaa
      121 tttgcgtaaa ataaagtgta atccctttga tcataaggga atgttgacaa ttagatcgat
      181 tctcctaggc tgactttcgc aataaatggg gttgtcaata attgtgtatg gtcgtttgcc
      241 gtccggcggc cgtcattccc gcgcaggcgg gaatccagaa acgcaacgca gcaggacttt
      301 atcggaaaaa accgaaatcc caccgccgtc attcccgcgc aggcgggaat ccagcaacac
      361 aacgcagcag gactttatcg gaaaaaaccg aaaccccacc gaccgtcatt cccgcaaaag
      421 cgggaatcca gcaaccgaaa aaccacagga atctatcagc aaaaacagaa accccaccgc
      481 cgtcattccc gcgaaagcgg gaatccagac ccgtcggcac ggaa
//
LOCUS       DAWKQJ010000281          511 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.281, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000281 DAWKQJ010000000
VERSION     DAWKQJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.281"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..101
                     /locus_tag="WJL20_002031"
     CDS             <1..101
                     /locus_tag="WJL20_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7684127.1"
                     /translation="KSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT          106 a          117 c          139 g          149 t
ORIGIN      
        1 gcaaatcttt cccgctgttc ttgaaagaat gcgaatttcg atttaacttc ggcacaccgt
       61 ctcaacagct taaaatcctg tgggatttag ggctaatcta gtacagcccc ttagatttaa
      121 ttcactatac ctgcgtaatg gtatcgggta ctcatcatca acctgccttg cattcggttg
      181 tgttgtccgg caactgtccg gttgtgtcga tgggttgccc tgccgctccc tgtgctgcgc
      241 aacggttgtg tgccttgggt gggcgcgggt ggcggccggg cggtttgttg ctatgatacg
      301 ggagttccga tatacaaggg tgggcttcag cccaccaatc aacgaccgaa aaagaaaaaa
      361 cgttgggctg aagcccactc tacgcaatta cgcaactgtt atcgtgggga tgacgggatt
      421 ttaggttttt gtttttctgt ttttgcggga aggatgggtt ttaggttgcg gtcatttatc
      481 ggaaaagcag aaatcgctcc gccgtcattc c
//
LOCUS       DAWKQJ010000282          511 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28919_Neisseria
            meningitidis SAMN10275975-rid8980983.denovo.282, whole genome
            shotgun sequence.
ACCESSION   DAWKQJ010000282 DAWKQJ010000000
VERSION     DAWKQJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275975
            Sequence Read Archive: SRR8200276
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676268.1
            Genome Coverage       :: 155x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 18:17:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,031
            CDSs (total)                      :: 1,979
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 39 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 10 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275975-rid8980983.denovo.282"
                     /isolate="M28919_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          161 a          110 c           80 g          160 t
ORIGIN      
        1 gttactaaaa ttttatgctc aatctcattt tcaaaatgca aaacttttct gatttttcct
       61 actttttgct caatattagg aaggttttag gcaattgaaa attttttggc gcatttttat
      121 gcgtcaaatt tcgttaacag actatttttg caaaggtctc atgcagatat tcgaatatcg
      181 aatttgcctt atgattacgg ttttctgtct tatctgcctt aaaacgccgg tattccgttt
      241 gaaccaaatt atatttgccg aaacgctcaa aaatctccct tacctgcccg acagacatca
      301 agccttcgtt gttgtagctg agaaaatata ttgaaagtcc gcatccctaa tcaaagcctc
      361 aaataccgga atcacatgcg attttgagca agtccgcgct aaaacacatc gtacagcgca
      421 aaaccggatg ggaatggcaa aatccggaaa caaacatttt tcaaagcata caaacaaaaa
      481 aataccaacc tccggttggt attccttaac t
//
